LOCUS       DAPIDM010000001        76152 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000001 DAPIDM010000000
VERSION     DAPIDM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 76152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.001"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(223..687)
                     /gene="bstA"
                     /locus_tag="RJE42_000001"
     CDS             complement(223..687)
                     /gene="bstA"
                     /locus_tag="RJE42_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDV5875465.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIGAILEGLQTLPER
                     ARKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            854..1192
                     /locus_tag="RJE42_000002"
     CDS             854..1192
                     /locus_tag="RJE42_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDV5875466.1"
                     /translation="MSVSDQIIKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKIFKSKWQALNKGMI
                     NLFGGENDVK"
     gene            1182..1949
                     /locus_tag="RJE42_000003"
     CDS             1182..1949
                     /locus_tag="RJE42_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875467.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEEEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFTLAVIIIPMFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIIGITFLILVIGTCILIKQKIYALIVLVISLPSLIARIASIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(2068..2706)
                     /gene="pcp"
                     /locus_tag="RJE42_000004"
     CDS             complement(2068..2706)
                     /gene="pcp"
                     /locus_tag="RJE42_000004"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDV5875468.1"
                     /translation="MHILVTGFAPFDNQDINSSWEAVTQLENIIGTHTIDKLKLLTSF
                     KKVDTIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHILYHLGYLQDTHYPHLR
                     FGFIHVPYIPEQVVGKPDTPSMPLEQIVAGLTAAIEAISDDDDLRIALGTTQ"
     gene            complement(2860..3816)
                     /locus_tag="RJE42_000005"
     CDS             complement(2860..3816)
                     /locus_tag="RJE42_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV5875469.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEED
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENEAK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            4064..5035
                     /locus_tag="RJE42_000006"
     CDS             4064..5035
                     /locus_tag="RJE42_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDV5875470.1"
                     /translation="MTQQDLPTLVYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKRPFVSHKANPAAIKIHKDGRLFICY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTTYCIDDMVFDSKGGFYFTDFRGYSTNSLG
                     GVYYVAPDFKTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMDGGSMLYTVNGFAKGHKSYQFQ"
     gene            complement(5181..5696)
                     /locus_tag="RJE42_000007"
     CDS             complement(5181..5696)
                     /locus_tag="RJE42_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDV5875471.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSITESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGIINREKYGINFNQTLETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5884..5991
                     /locus_tag="RJE42_000008"
                     /pseudo
     CDS             5884..5991
                     /locus_tag="RJE42_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            6109..7233
                     /locus_tag="RJE42_000009"
     CDS             6109..7233
                     /locus_tag="RJE42_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV5875472.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSANPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIQRDTKRWKDE
                     VESIVGKTDMFVFPHGAQDRHTLAYDYLVDKAGFKFIAGVGPNNFTDISTTNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            7538..8356
                     /locus_tag="RJE42_000010"
     CDS             7538..8356
                     /locus_tag="RJE42_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDV5875473.1"
                     /translation="MNNSEQLIALKEAETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            8374..8988
                     /locus_tag="RJE42_000011"
     CDS             8374..8988
                     /locus_tag="RJE42_000011"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV5875474.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            8981..10231
                     /locus_tag="RJE42_000012"
     CDS             8981..10231
                     /locus_tag="RJE42_000012"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV5875475.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKVYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFDQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            10247..11260
                     /locus_tag="RJE42_000013"
     CDS             10247..11260
                     /locus_tag="RJE42_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDV5875476.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSSEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTVFLTAIADKMKAINGYFVID
                     EAYLDYGKAYDVEMAPHILRMRTLSKAFGIAGLRLGVLMSTAETIQRIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            11229..11807
                     /gene="hisB"
                     /locus_tag="RJE42_000014"
     CDS             11229..11807
                     /gene="hisB"
                     /locus_tag="RJE42_000014"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDV5875477.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            11804..12382
                     /gene="hisH"
                     /locus_tag="RJE42_000015"
     CDS             11804..12382
                     /gene="hisH"
                     /locus_tag="RJE42_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV5875478.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKKNIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            12375..13079
                     /locus_tag="RJE42_000016"
     CDS             12375..13079
                     /locus_tag="RJE42_000016"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV5875479.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTEWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQKDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            13076..13834
                     /gene="hisF"
                     /locus_tag="RJE42_000017"
     CDS             13076..13834
                     /gene="hisF"
                     /locus_tag="RJE42_000017"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV5875480.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            13831..14463
                     /locus_tag="RJE42_000018"
     CDS             13831..14463
                     /locus_tag="RJE42_000018"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV5875481.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            15554..17596
                     /gene="lip1"
                     /locus_tag="RJE42_000019"
     CDS             15554..17596
                     /gene="lip1"
                     /locus_tag="RJE42_000019"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDV5875482.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPRDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRTKVAPSLNKSDQTSQREIVNETEIEKVQPQQKNQSNDKMTSH
                     HFNNEQEVNPQKDEKTLSVSDLKNNQKSPVEPTKNNDKKKGLNLLKSSAVATLPNKGT
                     KELTAKATGDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGSREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
     gene            complement(18044..19096)
                     /gene="icaC"
                     /locus_tag="RJE42_000020"
     CDS             complement(18044..19096)
                     /gene="icaC"
                     /locus_tag="RJE42_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDV5875483.1"
                     /translation="MKKIRLELVYLRAIICAIIIVTHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDTSFQKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLLILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(19083..19955)
                     /gene="icaB"
                     /locus_tag="RJE42_000021"
     CDS             complement(19083..19955)
                     /gene="icaB"
                     /locus_tag="RJE42_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDV5875484.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDPPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKSSEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(19952..20257)
                     /gene="icaD"
                     /locus_tag="RJE42_000022"
     CDS             complement(19952..20257)
                     /gene="icaD"
                     /locus_tag="RJE42_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDV5875485.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            complement(20221..21459)
                     /gene="pgaC"
                     /locus_tag="RJE42_000023"
     CDS             complement(20221..21459)
                     /gene="pgaC"
                     /locus_tag="RJE42_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV5875486.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHNPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            21623..22183
                     /gene="icaR"
                     /locus_tag="RJE42_000024"
     CDS             21623..22183
                     /gene="icaR"
                     /locus_tag="RJE42_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDV5875487.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            23030..23692
                     /locus_tag="RJE42_000025"
     CDS             23030..23692
                     /locus_tag="RJE42_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDV5875488.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            23709..24401
                     /locus_tag="RJE42_000026"
     CDS             23709..24401
                     /locus_tag="RJE42_000026"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDV5875489.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIEDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            24398..25165
                     /locus_tag="RJE42_000027"
     CDS             24398..25165
                     /locus_tag="RJE42_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDV5875490.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            25400..25900
                     /locus_tag="RJE42_000028"
     CDS             25400..25900
                     /locus_tag="RJE42_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5875491.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATPEALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVPANNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            25903..26379
                     /locus_tag="RJE42_000029"
     CDS             25903..26379
                     /locus_tag="RJE42_000029"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDV5875492.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDSPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNRQGNDIGEKYRTGLYSCVDDHLIE
                     AHQFIERRKDRDKIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISIDLLNKYK"
     gene            26458..27069
                     /locus_tag="RJE42_000030"
     CDS             26458..27069
                     /locus_tag="RJE42_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV5875493.1"
                     /translation="MHTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDTSVNAAPF
                     SFFNIVNNHPPMIAIAVQRVGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGLFTIDRPEK"
     gene            complement(27154..27513)
                     /locus_tag="RJE42_000031"
     CDS             complement(27154..27513)
                     /locus_tag="RJE42_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875494.1"
                     /translation="MILRYVTNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(27663..29755)
                     /locus_tag="RJE42_000032"
                     /pseudo
     CDS             complement(27663..29755)
                     /locus_tag="RJE42_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621844.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29742..30686)
                     /locus_tag="RJE42_000033"
     CDS             complement(29742..30686)
                     /locus_tag="RJE42_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875495.1"
                     /translation="MRKVTGFVLCTSLILGGCSFMHKDHTKDIPKTVSVKDYDGKYIG
                     EHKKRNEVFLKKHKDEAIKKYKDYVKDTFGYDCKVNLVEAYTNKSGFSEKSKTDGLVV
                     VGTVNYDIPFQFRLIFVESDNGITITTFTPGHKNETSAAVAAMMYKHYEHDIEQARLK
                     FKSEVEKNGYYAMNEKLQKKQEFNGVTKQYLNFNTVSIDDLDKFKKEFKPVMHLKGDA
                     FNQQLQNLINKYPQIQKNMKSEFIAYYDKDANKETVADYAWSLKKPTNEIMKTYPGEK
                     RMRFYKDKVSPYELDQYGRLNPDADEIYVIGGNYNEKK"
     gene            complement(30873..31808)
                     /locus_tag="RJE42_000034"
     CDS             complement(30873..31808)
                     /locus_tag="RJE42_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875496.1"
                     /translation="MRKIMQILLCTSIIVSGCSFIPKYQTKDIPKTVSVKDYDGQYIG
                     EHKKRNEVFLKKHKDEAIKKYKDYVKDTFGYDCKINLVEAYTNKSGFSEKSKIDGLVV
                     VGTVNYDVPFQFRLIFVESGNGIAITTFTPGHINETSAAVSAMMYKRYEHEIEQARLK
                     FKSEVEKNGYYAMNEKLQKKQEFNGVTKQFLNFNTDSIDDLKKFKQEFKSVMHLKGDA
                     FNQQLQSLINKYPQIQKNMESEFIAYYDKGENRETVANYVWNLQKTTNDTMKLYPGNK
                     SIIFYKDKVSATQLDEHKRLQSDSQEISISGGENN"
     gene            32122..32814
                     /locus_tag="RJE42_000035"
     CDS             32122..32814
                     /locus_tag="RJE42_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV5875497.1"
                     /translation="MNYSPKQGVKSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGFRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIRPIARLGYYDYTVVNEIFEMKAPSASKEELAGLE
                     GRNFDNQSDDKE"
     gene            33361..40200
                     /gene="sasA"
                     /locus_tag="RJE42_000036"
     CDS             33361..40200
                     /gene="sasA"
                     /locus_tag="RJE42_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDV5875498.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSIVSQDNQSISKKMTGYGLKTTAVIGGAFTINMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSENVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQRTNISTNQSTASNTTSQSTAPAPTH
                     LKQTSTTSTSTAPVKLRTFSRLAMSTFASTATTSAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSTPNVSAKAKADPPSVAGGGAF
                     GAFVTTDSTGVASTYTSSSIADNAAKLNIQPTNNAFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQTNNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     SSTEKSESQSTSTSDSLRTSTSLSDSVSMSTSGSLSKSQSLSTSTSDSASTSQSVSDS
                     TSNSISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIATSQSVSTSTSDSMSTSEIVSDSISTSGSLAASDSKSMS
                     VSSSMSTSQSGSTSESLSDSISTSDSDSKSLSLSTSQSGSTSTSTSTSSSVRMSESQS
                     TSGSMSTSQSDSTSISTSFSDSTSDSKSASTASSESISQSVSTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSTSDSTSTSDSISEAISGSESTSISLSESNSTSDSES
                     KSASAFLSESLSESTSESTSESLSSSTSASTSLSDSNSESGSTSTSLSNSTSSSTSIS
                     TSTSGSASTSAYKSESVSTSLSTSTSTSLSDSTSLSTSLSDSASGSKSNSLSASMSTS
                     DSISTRKSESLSTSTSLSGSTSESESGSTSSSESKSDSTSMSLSMSQSTSGSTSVSTS
                     ESLSDSTSTSLSLSASMNQSGIDSNSASQSVSASTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSMSLSDSTSMSRSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESTSTS
                     LSDSESTSNSTSESTSNAISTSASASESDSSSTSLSDSTSASMQSSESDSQSTSTSLS
                     NSQSTSTSNRMSTITSESVSESTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTLDSQSMSLSTSTSTSVSDSTSLSDSVSDSTSDS
                     TSTSTSGSMSASISLSDSTSTSTSASEVMSVSISDSQSMSESVNYSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESSSLSDSISMSDSTS
                     TSESDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSVSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     ISVSDSSSTSTSESLSDSMAQSQSTSTSASGSLSTSISTSMSMSASTSTSQSTSVSTS
                     LSTSDSISDSTSISISGSQSAVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSTSESLSMSGSDSTSVSDSTSMSESDSTSVSMSLSTSDSTSISDSESVSTSTSMS
                     LSTSESLSTSMSGSQSISDSTSTSMSNSTSMSNSTSTSMSGSTSTSESNSMHPSDSMS
                     MHHTHSTSTSRSSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNG
                     LLGGVMTLLVGLGLMKRKKKKDENDQDDSQA"
     gene            40329..41540
                     /gene="secY2"
                     /locus_tag="RJE42_000037"
     CDS             40329..41540
                     /gene="secY2"
                     /locus_tag="RJE42_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDV5875499.1"
                     /translation="MLKLLQQYEYKIIYKRILYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLITLVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMRSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKDVSDDMPMMTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            41551..43104
                     /gene="asp1"
                     /locus_tag="RJE42_000038"
     CDS             41551..43104
                     /gene="asp1"
                     /locus_tag="RJE42_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDV5875500.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNNTEFDDMISLMGMHLEN
                     DLDYRLIVLNHAPNLRTFLHRYDLYETKYWSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLFENFKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDVD
                     LQQMMQRLVDYIAQEDNYRLILLTRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            43094..44662
                     /gene="asp2"
                     /locus_tag="RJE42_000039"
     CDS             43094..44662
                     /gene="asp2"
                     /locus_tag="RJE42_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDV5875501.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYSFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIEETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNCSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            44640..45599
                     /gene="asp3"
                     /locus_tag="RJE42_000040"
     CDS             44640..45599
                     /gene="asp3"
                     /locus_tag="RJE42_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDV5875502.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDNLEYKSMRVTKIG
                     DDQYGNDRINVIIAEPSDAYPTISNDFLKSFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWFTEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            45589..47979
                     /gene="secA2"
                     /locus_tag="RJE42_000041"
     CDS             45589..47979
                     /gene="secA2"
                     /locus_tag="RJE42_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDV5875503.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDVLKQKTL
                     EFKERIASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVFFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSKTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKVLAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQIALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAN
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            47997..49505
                     /gene="gtfA"
                     /locus_tag="RJE42_000042"
     CDS             47997..49505
                     /gene="gtfA"
                     /locus_tag="RJE42_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDV5875504.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFEDQEVIWLYQYFSDISIAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNENGSIAYNEYIDGDNSIYVFADAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYTD
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVVAKLAHAIVMYFNNGPQTPHYISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            49498..50856
                     /gene="gtfB"
                     /locus_tag="RJE42_000043"
     CDS             49498..50856
                     /gene="gtfB"
                     /locus_tag="RJE42_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDV5875505.1"
                     /translation="MIKLFDYFNGHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVL
                     VWQEQCDGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVKSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAF
                     ES"
     gene            51098..52993
                     /gene="sasF"
                     /locus_tag="RJE42_000044"
     CDS             51098..52993
                     /gene="sasF"
                     /locus_tag="RJE42_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDV5875506.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASENDTE
                     ISKEILSKQDLLDKVDKANRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKGSQIEMNGQSDIDSKLDQLLKDLNEVSSKVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVDKALEDLDHLNQQIRKSKDASKDPVVSTTDNNHEVAKTPNN
                     DGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLN
                     TLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYATQRIL
                     ESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSL
                     LISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNN
                     AKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNDKADDLLNLQK
                     RLNQTKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPDIPTP
                     KPEKTLTLGKGNGLLSGLLNADGNVSLPKAGEAIKEHWLPISVIVGAMGVLMIWLSRR
                     NKLKNKA"
     gene            53161..53721
                     /locus_tag="RJE42_000045"
     CDS             53161..53721
                     /locus_tag="RJE42_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDV5875507.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(53988..55847)
                     /locus_tag="RJE42_000046"
     CDS             complement(53988..55847)
                     /locus_tag="RJE42_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDV5875508.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSTDDETSKDTTSKDTDKADNNNTSNQDNNDKKVKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQSNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFKGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINASAAEELSYITGK"
     gene            56058..59039
                     /locus_tag="RJE42_000047"
     CDS             56058..59039
                     /locus_tag="RJE42_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDV5875509.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPVAGNGVASTPPSAPNGNTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     SILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLEPVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(59151..60089)
                     /gene="manA"
                     /locus_tag="RJE42_000048"
     CDS             complement(59151..60089)
                     /gene="manA"
                     /locus_tag="RJE42_000048"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDV5875510.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDYYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLAV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(60103..62055)
                     /locus_tag="RJE42_000049"
     CDS             complement(60103..62055)
                     /locus_tag="RJE42_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDV5875511.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQSIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVIVGLIFIFL
                     IGAPISSIFEALTTWLKGMQGANIIILALIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANMIGAMIASVLAAIGGVGDRVAHGGPIVAVLGGIDHILWFIFAVIIGSL
                     VTMITVLLLKRRTPVIAVDAPAQHTQLHATDIIQHDTEVDNVDNTAKHSHLNKPSHVF
                     NQQTIIIADQNMSRNEAIDTLIHQLKVCRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(62132..64006)
                     /locus_tag="RJE42_000050"
     CDS             complement(62132..64006)
                     /locus_tag="RJE42_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDV5875512.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIIVDGTDNDITNAIIHLN
                     QLSTGHVNVEDLILNELPDAHRRMMSHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIQQINTRLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVSPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKPRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(64246..64689)
                     /locus_tag="RJE42_000051"
     CDS             complement(64246..64689)
                     /locus_tag="RJE42_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875513.1"
                     /translation="MKKFLITIVIILLIGALGFAIYAAINHSTSSNHNNEQDNKATHQ
                     KKDTEVNHQKDENTNNTNENQNNNGVTNGNQSSTPSTVQNNGSTAPANTQPSNPNQSN
                     SNHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            64944..65471
                     /gene="isaB"
                     /locus_tag="RJE42_000052"
     CDS             64944..65471
                     /gene="isaB"
                     /locus_tag="RJE42_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDV5875514.1"
                     /translation="MNQTTKLCAATTLALGTLIGATVVGTASPIHQEAQAATTPYYTY
                     HGYIGHNANFIMDKHFINAIKYDNVTFNGIKLAKTNATKKVEKYDQTFKGVTAKGNEA
                     NQLQFIVKNNISLKDIQKAYGKDLKKENNKTKESDSGIFYYQHSKKSLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            65885..67414
                     /gene="aur"
                     /locus_tag="RJE42_000053"
     CDS             65885..67414
                     /gene="aur"
                     /locus_tag="RJE42_000053"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDV5875515.1"
                     /translation="MRKFSRYAFTSIATVTLLSSLTPAALASDTNHKPATSDINFEIT
                     QKSDAVKALKELPKSENVKNHYQDYSVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDEAADKAFNAVKIDKNKAKNLQDDVIKE
                     NKVEIDGDSNKYIYNIELITVTPEISHWKVKIDADTGAVVEKTNLVKEAAATGTGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAYTFNDQTGQATLITNEDENFVKDDQRAGV
                     DANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            67782..68231
                     /locus_tag="RJE42_000054"
     CDS             67782..68231
                     /locus_tag="RJE42_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDV5875516.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFKHYCEHEVLSSIINGAYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNEMAEYVYAKLFR"
     gene            68579..69814
                     /gene="arcA"
                     /locus_tag="RJE42_000055"
     CDS             68579..69814
                     /gene="arcA"
                     /locus_tag="RJE42_000055"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDV5875517.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKEVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            69847..70857
                     /gene="argF"
                     /locus_tag="RJE42_000056"
     CDS             69847..70857
                     /gene="argF"
                     /locus_tag="RJE42_000056"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV5875518.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            70948..72378
                     /gene="arcD"
                     /locus_tag="RJE42_000057"
     CDS             70948..72378
                     /gene="arcD"
                     /locus_tag="RJE42_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDV5875519.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLASIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            72395..73336
                     /gene="arcC"
                     /locus_tag="RJE42_000058"
     CDS             72395..73336
                     /gene="arcC"
                     /locus_tag="RJE42_000058"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV5875520.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            73435..74139
                     /locus_tag="RJE42_000059"
     CDS             73435..74139
                     /locus_tag="RJE42_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDV5875521.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            74489..>76152
                     /gene="clfB"
                     /locus_tag="RJE42_000060"
     CDS             74489..>76152
                     /gene="clfB"
                     /locus_tag="RJE42_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDV5875522.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTAKPMST
                     QTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTKDANSIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLTVAEPVVNAADAKGTNVNDK
                     VTASNLQLQKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTGEFNNRIFYEHPNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPVDPTPGPPVDP"
BASE COUNT        26874 a        11589 c        13819 g        23870 t
ORIGIN      
        1 catgggtatt tataggtata ttgggactgt atttaatttt aagaaaaaga tttaactcat
       61 aaaccattat aattattttt atagataagg ctattcttag ttctatgtat aatacatgat
      121 attaataggt cacttttaat ctgtatgtaa gcagactaag agtggccttt taaaaaataa
      181 aaaaatccgt taacgtgttg ttaacggtta ataagcacat atttatttta aattttttaa
      241 tatacgagac atctcttcaa ttttaccagc atgaagtggg atgtgaatgg catttaatac
      301 taataactct tctaagttat tacaaccgac aattggctgt ttcaattcaa ttgctaaatc
      361 atcttcagtt agttttcgtg cacgttcagg taatgtttga agaccttcta aaatcgctcc
      421 aatactaggt acttcgtcct tccattcatc tggagacgaa ccatttccga ataaaggtct
      481 atatatattt aaatcaatat tctctttacc agcaacagct aaggccgatt caaaaattgt
      541 taatacatgc ccaaattgcc aatgaatggt attagaaaaa ggcttatgat aatcatcaag
      601 taccttttca actttccaag tatcatacac atttactaaa tatcctaaac ccatatcaat
      661 gacatcaaat accgtttttg tagtcattat taattcctcc cccataaata aaacatactt
      721 ccgtaagtat taccctatta acaaagttgt tagtcattta aataatgtgt acaacaacta
      781 acttataata taagatagtt gacgaaaagt tctcaatgta ctatcttatt atgtaagata
      841 gtaggtgatt aaaatgtcag tgtcagatca gattataaaa ggtctcttag atggggcgat
      901 attaggtctc attgcccaag gcgaaacgta tggttatgaa attatgacga aacttaagaa
      961 tcaagagttt ccagagataa gtgagggtag tatttatcca gttttaatgc gtttaaacaa
     1021 aaaaggatac gttgatacga caataaaaaa atctagtaat ggtggcccaa ggcggaaata
     1081 ctatacgatt accgactcag gtttaaagga actaaaaata tttaaatcga aatggcaagc
     1141 tttgaataaa gggatgatta acttgtttgg aggggaaaac gatgttaagt aaacaatctc
     1201 aagatttttt agaaaaatta cgtgttgaat tgttatttag aggtaaaact gaagaagaag
     1261 tagaagaact attggaagag ctcgaagatc atttaactat ggctgaaaaa aataacgaag
     1321 atacaagttc aattattaat acaccgatta agtcaatggc tgatcagtta tcacctgaga
     1381 taagtcttac tactggttta tataaatata ttacgttgta cttcgctttt actttagcag
     1441 tcattataat tcctatgttt ttagatttgg gtacattcga tgtgacaatt gcatttttat
     1501 tgtatatagc tagtatcgtt attttaggtg ttgtcattgg tatgctgatt ctacgcaata
     1561 cattagtccg atttggtgac caaaaaataa catatgttat aaatgtgact tatggaatta
     1621 tcgtattttt atggatgatt ttaggtggct tgattattaa aaaatatcct atctatagtt
     1681 tctttgagtt aagtgacaaa cagagtttca ttattgggat aacattttta atattagtta
     1741 ttggaacttg tattttaatt aaacagaaaa tttatgcgct catcgtgttg gtaatttcat
     1801 tgccaagttt gatagcaagg atagcatcta tctttagtaa agaaccagac acttttaatt
     1861 tagtttcagt aagtatactc atcattctaa atatagcatt tattatttat acatttgtaa
     1921 attttaggaa gttaaaaaaa gaagactaaa caatgtcgtt tagctaaaat acatcattat
     1981 agtaaaactg tataccaatc atccaaaagc accctaaagt tagacttaaa ctaacaaatt
     2041 agggtgcttt tgtttgtgca tttataacta ttgcgttgtg cctagagcta tacgtaaatc
     2101 atcgtcatca gaaatagctt caatggctgc agtcaaacct gcaactatct gttctaatgg
     2161 catagatggt gtatcgggtt taccaacgac ttgctctggt atatatggca catgaataaa
     2221 tccgaatcga aggtgagggt aatgcgtatc ttgtaaataa cctaagtgat aaagtatgtg
     2281 attacaaaca aatgtacctg cactatttga aagtgctcca ggaagtcctt ggttaataat
     2341 actttgagtc attgctttaa ctggtaaatt tgaaaaataa gctggcgcac cgtctaaatg
     2401 aatggcttga tcaataggtt gaaaatcatc attatctgga atacgtgcat catcaatatt
     2461 aatggcgaca cgttctgggg taatggcatt tctaccacca gcttgtccta tagctagtac
     2521 aacatcataa tgattagatg ccaacgtttt atttataata gtatctactt tcttaaaaga
     2581 ggttagtagt tttaatttat cgattgtatg tgtgccaata atattttcta gttgagtcac
     2641 agcttcccaa gaagaattaa tatcttgatt gtcaaaaggc gcaaaccctg ttactaaaat
     2701 gtgcatttta agacaccccg cagtattaaa atttaattta gtataacatg tgcataatta
     2761 aaaacatatc aggttatgca tgcgcataaa aaaaacagcc ttgaaaaacc tttataaatc
     2821 ggattttcaa agcgtgtttt gaaaggtatt taattaatac taagcatgtt gatgtaaatc
     2881 atcaaagttt gttaaacgtt gttgccattc gttgtcacta atattgtttg cctgaacata
     2941 acgattacgc tcatgtttag cacattcata agagcatgca cctaaatatt tggcttcatt
     3001 ttcttctgaa actaatattt gcttattaca ttcaggattg gcgcaattaa tatagcgttc
     3061 acatggtttg ccatcaaacc aatccttacc aataatcgtt ttttcaactt ggttgatatc
     3121 aacactgata cgatcatcaa atacatacat tttaccgtcc caatattgac cttttgtttc
     3181 agggtcttta ccatatgtag ctataccgcc gtgaagctgc gctacatctt cgaaaccttc
     3241 ttttaaaagc catccagaaa atttttcaca tcgaatgcca ccagtacagt acgtaaccac
     3301 ttttttatct gcaaataacg ctttattctc tttaatccag tcaggcaaat ctctaaagcg
     3361 cgtgatattt ggacgaattg cacctcggaa atgacctaaa tcaaattcat aatcattacg
     3421 tgcatcaata atgactgtgt catcatcttc aagagctttt ctaaattcta caggtgataa
     3481 atattggcca gttgtgtgtc ttggatcgac gtcttcttct aaatctaaag caacaatctc
     3541 ttttcgagga cgtacatgca ttttcttaaa agcatgtcct tcagcttcat caattttaaa
     3601 caccatatct ttgaatcgtt catcggcatg catatgtgcc atatattgtt cggtttcttc
     3661 ttttgtacca gataatgtgc cgttaatacc ttctgtagaa acaagaattc taccttttaa
     3721 atggtgtgct ttacaaaagg ctaagtgatc ctgagcaaac tgttcagggt catcaatcgt
     3781 cgtatattta taatataaaa gaacttgata gttcataggt taatctcctt ctaaaaacat
     3841 gttataagca tgttaatttt taaaataaag cgtcaatacg catatttgtc tacgtccatc
     3901 attttaacag agttgtattg tcattcaata tttctagctt gaaatcagcc actagaccga
     3961 taaaatagat aaatgaaaac gttatcattt gatttgatag ttaccttgtt taaaatgaaa
     4021 atgatagtta aaacgaatga atgaaatgaa aggaatgatt atgatgacac aacaagattt
     4081 acctacatta gtttatagcg ggaagtctaa cagtgctgtt ccaattatat ctgaaagtga
     4141 attacaaaca attacagctg aaccatggct tgaaatttcc aagaaaggat tgcaactaga
     4201 aggattgaac tttgatcggc agggacaact ctttttattg gatgtattcg aaggaaatat
     4261 ttttaaagta aatccagaaa ctaaagaaat taagcggcct tttgtaagcc ataaagcaaa
     4321 tcctgcagca atcaaaatac ataaagatgg ccgattattt atttgttatt taggagattt
     4381 taaatctaca ggtggtattt ttgcagcgac agaaaatggt gacaacttac aagatattat
     4441 tgaagatctt tcaacaacat attgtattga tgacatggta tttgattcta aaggtggatt
     4501 ttattttaca gattttagag gatactctac caattcacta ggaggcgttt attatgttgc
     4561 gccggacttt aaaacagtga cgccgattat tcaaaatatt agcgtagcaa atggtattgc
     4621 tttaagtaca gatgaaaaag tactatgggt aacagaaaca acagccaatc gattacatcg
     4681 cattgcactt gaagatgatg gtgtgacgat acaaccattt ggagctacca taccgtacta
     4741 ttttacaggt catgaaggac cagactcatg ttgtattgat agtgacgata atttatatgt
     4801 ggcaatgtat ggtcaaggcc gagtattagt atttaataaa aggggttatc caataggaca
     4861 aatattgata ccagggcgtg atgaaggtca tatgttacgt tctacgcatc cgcaatttat
     4921 acctggcaca aatcaactca tcatttgttc caatgatata gaaatggacg gaggatctat
     4981 gctttatacg gttaatggct ttgcaaaagg gcataaaagt tatcagtttc aataagacct
     5041 gcctgaatga agggcgtctt aatataagtt taaatgttaa aatagtgaat cataaattaa
     5101 agaaaaatgc caactatacc tagagatgat atagttggca tttcgtatat attaaaatca
     5161 atgaggatta tgagatgagg ttattctgag attgagaatt cagctgatgc ttcgaattta
     5221 acatctttac ctagcattac tccaccagtt tcaagtgttt ggttaaagtt aatgccatat
     5281 ttttctctgt tgattatacc agtaacaata atacctgtta cttgagaacc atccatagga
     5341 tttttactta cgccgttaaa ctcaacgtcg aatgtttctt cgttagtgat acctttaatt
     5401 gttaaatcac caacaacttt gctttctgta attgattttg tcacaaaagt gattttatca
     5461 aattcatcag taccaaagaa atcaccagat tttaagtggt tatcacgtgc ttcgttttta
     5521 gtgttaattg agcttggaat aattgttgca gtagctttta aagtgctgaa gtcattaata
     5581 tctccttcaa cagcgacatc aaattgatcg aatgaacctt tcactttaga aaccattaaa
     5641 tgtttaattt ggaactctaa actactgtgt gcaccatcaa aagtaaagtt tgtcataata
     5701 taaaacctcc atatttttat ctcttagata tattttttat acttgatata catacaatac
     5761 cacccataaa acaaagatgc aagcatttta tttcgaattc gaatattttt ctgtgctgcg
     5821 tttaaagaat gacctataaa acaccacact attgttatac cctagtcaat taattcaaac
     5881 ataaataagt gtaaccaacg ttcaataaat ttagcagaaa ggcttaattt caagcgagac
     5941 gatacaatgg acgatattat caatcaagga gatattgtgt tttataaata aaatactatg
     6001 acattatcta aaaaataaaa ttaaaatccc aacagtttat taaaaagtta tgagccacat
     6061 aataaaaatg ttataattgt gcataataaa acatagggag aatgtttaat gcataaaaaa
     6121 tatttattgc tattttttct gttattatca gttgcattgt tatatagctg ttccaagcaa
     6181 aatgagaaag ctcaaaataa atctgctaat ccaaatgagt atgcaaagca taaatcgaaa
     6241 atggatgaac attgggaaac atatcacggc caagtttatc atgtgtttta tcatccatta
     6301 attacagacc caaaagttgc gtttacagga gatacacacc aagctaaggg caacaatgat
     6361 tggatgatta ctgtaagtga atttaaaaaa tcattgaacg aattatataa aaataattac
     6421 atcatcgtta atccacatga tgtatacgac ttatcatctc atcatgtaaa agccaaaaaa
     6481 ataaaattgc cgaaaggtaa aaagccacta atcatctcaa ttgatgatat gaattactat
     6541 agttacatga gacaccatgg ttatgctgac cgcttagtac ttgataaaaa taagcatgtt
     6601 gtatctgaaa cgacagactc aaaaggccat gctacttatt ctgatgacaa tgatatcgtg
     6661 ccaattttaa ataaatttgt aaaagagcat ccagattttt ctttaaatgg tgaaaaaggt
     6721 gttgttgctt taaccggtta tgagggcgta ttaggttatc ggacgaatga attaacgagt
     6781 aaagattata caaagaataa aaaagcagct gaagaagttg ttcgcgcaat gaaacgggat
     6841 ggatggtcat ttgcgagtca ttcatatggt cacattaatt ttgaaaaaac atctttggag
     6901 ggtattcaac gtgatactaa aaggtggaaa gatgaagttg aatcaattgt agggaaaaca
     6961 gacatgtttg tatttccaca cggtgcacaa gataggcata cattagcgta tgattatttg
     7021 gtcgataaag caggctttaa atttattgca ggtgttggtc caaataattt tacagacata
     7081 tcaacaacga atgtttatca agatagggtt gcgattgatg gtctaaactt atttgaattt
     7141 aaatataaac tcaaaccgtt ttttaatcct gaaaatgtat atagtaaaca agatagacaa
     7201 ttctttaaag gtaatcggga ttatgaagaa tagttaaatt taataaaaag atagaaagaa
     7261 aataacttta cataataagg caaggacaat atgtgcaaga agtacaactt caatataaaa
     7321 atcttggtta agcattttta taggcacaaa tgtcttgcct tttttatttt aaaaaatttt
     7381 aaaatcaata caccgaattt tctgaaaaag gttgtaatgt attgctaaaa atgataagtt
     7441 attaatcaca ttacacttta gcgtggtaga gagataaagt atatgacgga aataccaagt
     7501 gattttaaat atagtattga aagtaaaggg tggtcgagtg aataattcag aacaattaat
     7561 tgcgttaaaa gaagcggaaa cagcattttt aaaatatttt aataaagcgg attatgagtt
     7621 agttgatttt agtgtggttg aaaagttgga ctggaaacaa ttgaaccatg aggatttgca
     7681 gcagatgggt gagcgaaatt tttggcaaca tgagcatcaa atttatgcac ttcgaaatga
     7741 ttttacagat caattattgc gttattactc aatgtatcca actgcagcaa caaaagtggc
     7801 atacacaggt ttaatcattc ggaataatga agcggctgta caagttggtt tggaaaacta
     7861 tgcaccatca ttagcgaatg tacaacagag cttaaagcta tttattcagt ttattcaaca
     7921 gcagttgaga gacaacgtgc attttgttgt attgggacat tatcaattgc ttgatgcctt
     7981 actcgataaa tcgttacaaa caccagacat actatcaatg attgaggaac gtaatttatc
     8041 gggactggtt acatatttgt cgactgaaca tccaatcgtg caaatcttga aagagaatac
     8101 gcaacaacaa ttaaatgtat tggaacacta tattccaaat gaccatccag cactcgttga
     8161 attaaaaata tgggaacgtt ggttacatac acaaggttac aaagacatcc atttagatat
     8221 tactgcgcag ccacctagat cctattacac aggtttattt attcaatgtc attttgctga
     8281 aaatgaatct cgagttttaa caggtggtta ttacaaagga agcatcgaag ggtttggatt
     8341 gggtttaaca ctttaagtaa gggagtatgc acaatgttaa gaattgccat agccaaagga
     8401 cgtctaatgg atagtttaat taactattta gatgtaattg aatatacgac attatcagaa
     8461 acattaaaaa atagagaacg ccaattatta ttaagtgtag ataatataga atgcatttta
     8521 gtaaaaggaa gtgacgtgcc aatctatgtg gaacaaggaa tggccgacat aggcattgtt
     8581 ggtagcgaca tattagatga gcgccaatat aatgttaata atttgttgaa tatgcctttt
     8641 ggagcatgtc attttgcggt tgcagcgaaa cctgaaacga ccaattatcg taaaatcgca
     8701 acgagttatg ttcatactgc tgaaacatat tttaaatcaa aaggtattga tgtcgaattg
     8761 attaaattga atggctctgt tgaattggcc tgtgttgtag atatggtaga cggaattgtc
     8821 gacatcgttc aaacaggtac tacgctaaaa gcgaacggac tggttgaaaa gcaacatatt
     8881 agtgatatca atgcaaggtt aataactaat aaagcagctt attttaaaaa atcacaatta
     8941 atagagcaat ttattcgctc tttggaggtg tctattgcca atgcttaatg cacaacaatt
     9001 tttaaatcaa ttttcattag aagcaccatt agatgagtct ttgtatccaa ttattcgcga
     9061 tatttgtcag gaagttaagg ttcatggaga taaagctttg aaaatgtata atctaacatt
     9121 cgatcatacg aaaacagatc atttagaaat tagtcatgaa caaattaaag cagcatttga
     9181 cacattagat gaaaaaacaa aacaagcatt acaacaaagt tatgaaagaa ttaaagtata
     9241 tcaagaaagt attaagcaga cgaatcaaca gttagaagaa tcagtggagt gttatgaaat
     9301 ataccatcca ctagaaagtg tgggtattta tgtgcctggt ggcaaagcaa gttatccatc
     9361 aacggttcta atgacagcga ctttagcaca agtagcgggt gtagaaaata ttgttgttgt
     9421 gacaccacct caacctaacg gagtatccca agaggtatta gctgcatgtt atattacgca
     9481 agttaatcaa gtgtttcaag ttggtggcgc tcaaagtatt gctgcattga cttatggaac
     9541 agaaacaata cctaaagttg ataagattgt aggtccagga aaccaatttg ttgcatatgc
     9601 caaaaaatat ttatttggac aggtaggtat tgatcaaatt gcaggaccaa cagaaatagc
     9661 actgattatt gatgacaccg cagatttaga tgccatcgta tatgatgtat ttgcgcaagc
     9721 agaacatgat gaattagcac gtacatatgt cattagtgaa gatgcgcaag tccttaaaga
     9781 tttagaatca cgtattgcta aagcattgcc taatgtggac agatacgaca ttgtttctaa
     9841 aagtatcgct aatcaacact accttatcca tgctagtaat tttgatgaag catgccatgt
     9901 catgaataca atcgcgcctg aacatgcgtc gattcaaaca gtaaatcctc aaccatatat
     9961 tgaaaaagtg aaatatgtgg gcgcattgtt tattggacat tattcgccag aggtcatagg
    10021 agattacgtt gcaggtccaa gtcatgtatt acctacaaat agaacagcta gatttaccaa
    10081 tgggttatcg gtcaatgatt tcttaacacg gaacacggtc attcatttat caaaagatac
    10141 atttgatcaa attgctgatt cagcacaaca tattgctcat gttgaagcat tatacaatca
    10201 ccaacagtct attttaatac gtcagtctta ggggagtgta aatgaaatga tttatattga
    10261 taaaaatgaa agcccagtta cgccgttgga tgaaaaaaca atgacgtcta ttattagtgc
    10321 aacgccatat aatttatatc cggatgcagc atatgaacaa ttcaaggaag cttatgctaa
    10381 attttacgga ttatcgtctg aacagattat tgcaggaaat ggctctgatg aattgattca
    10441 aaagttaatg ctgatcatgc cagaaggtcc ggcgttaacg ctaaatccag atttttttat
    10501 gtatcaagca tatgcggcac aagtaaatcg tgaaattgca tttgtagatg caggatcaga
    10561 tttaacgttt gatttggaaa ccattttaac gaaaatcgat gaagtacaac catcattttt
    10621 tattatgagt aatccacata acccttcagg caagcaattt gatacggtat ttttaacagc
    10681 tattgcagat aagatgaaag caataaacgg atactttgtc attgatgaag catatttaga
    10741 ttatggcaag gcatatgatg ttgaaatggc accacacatt ttaagaatgc gcacattatc
    10801 aaaggcgttt ggaattgccg gcttaaggtt gggtgtatta atgagtactg ctgaaacgat
    10861 acagcgtatt caaaaaatag aacatccata tccattaaat gtatttacgc taaatattgc
    10921 gacttatatt tttagacata gagaagagac aagacaattt ttaacaatgc aacgacagtt
    10981 agctgagcag ttaaaacaaa tatttgatac acatgttgca gacaaaatgt cagtattccc
    11041 atcaaatgct aattttgtac ttactaaagg ttcagcggcg caacaattag gacaatacgt
    11101 atatgaacaa ggatttaaac ctcgttttta tgatgagccg gtgatgaagg gctatgtaag
    11161 atattcaatt gcaacagcat cacagttaaa gcaattagaa gaaattgtta aagaatggag
    11221 tgcaaaatat gatttatcaa aaacaacgaa acacagctga aacacaacta aatatttcaa
    11281 tatcagatga ccagtcacca tcgcatatta atacaggtgt gggcttttta aatcatatgt
    11341 taactttgtt tacgtttcat agtggactgt cgttaaacat cgaggcacaa ggtgatatcg
    11401 acgtagatga tcaccatgta actgaagata tcggcattgt tattggccaa ttgttacttg
    11461 aaatgattaa agataaaaag catttcgttc gttatggaac aatgtatatt ccaatggatg
    11521 aaacattagc acgtgttgtt gtggatataa gtgggcgtcc atacttatca ttcaatgcat
    11581 cattaagtaa agaaaaagta ggcacgtttg atacggagtt agtagaagaa ttttttagag
    11641 cggtcgtaat caatgcaaga ttaacaacgc atattgattt aattcgtggt ggtaatacac
    11701 accatgaaat tgaagctata ttcaaagcat tttcccgtgc attaggcata gcgctaactg
    11761 caactgatga tcagcgtgtg ccgtcatcga aaggtgtgat tgaatgattg tcatcgttga
    11821 ttatggatta gggaatatta gtaatgtaaa acgcgctatt gaacatttgg ggtatgaggt
    11881 ggttgtctca aataagaaaa atataatcga tcaagcagaa acaatcatat taccgggtgt
    11941 cggtcatttt aaagatgcga tgtcagagat aaagcgatta aatctcgatg caatattggc
    12001 taagaatact gataagaaaa tgatcggtat ttgtctagga atgcagttaa tgtatgagca
    12061 tagtgatgaa ggcgatgcat ctggattagg gtttatccca ggaaatattt cgcgtatcca
    12121 aacagaatac ccagtgccgc acttaggttg gaataattta gtgagtaagc atcctatgtt
    12181 aaatcaagat gtttacttcg tacattctta ccaagcgccg atgtcagaaa atgtcattgc
    12241 atatgcgcag tatggtactg atattccagc aattgtccaa tttaacaatt atattggtat
    12301 tcaattccat cctgaaaaaa gcggtacata tgggttacaa attttgcgtc aggcgataca
    12361 agggggattt ataaatgatt gaattatggc cagcgattga tttgattggg tcaacaagtg
    12421 tgaggttaac agagggtaaa tatgatagtg aagaaaaaat gtcgcgttcg gctgaagaaa
    12481 gtattgctta ctatagtcaa tttgaatgtg tgaatcgtat tcatatcgtc gacttgatag
    12541 gtgctaaggc acagcatgcc cgagagtttg attatattaa gtcattgagg agattaacaa
    12601 ccaaagatat tgaagtaggt ggtggcattc gtacgaagtc acaaatcatg gactactttg
    12661 ccgcggggat taattattgc atagttggaa cgaaaggcat tcaagataca gaatggctta
    12721 aagagatggc acatacattt ccaggtcgca tttatttatc tgttgatgct tatggagaag
    12781 atattaaagt gaacgggtgg gaagaggaca cagagttaaa tttatttagt tttgtgagac
    12841 agttatcgga tatacccctt ggaggcatta tatatactga tattgctaaa gatggcaaaa
    12901 tgtccggacc taactttgaa ttaactggtc aattagtaaa ggcaacgacg attcctgtca
    12961 tcgcgtctgg tggtattaga catcagaaag atattcaacg attagcgtcg ctaaatgttc
    13021 acgctgctat tataggaaag gctgcacatc aagcatcatt ttgggagggg ctaaaatgat
    13081 taaaaaacgt atcattccat gtttagatgt caaagatggt cgtgtcgtta aagggattca
    13141 atttaaagga ttaagggata ttgggaatcc tgttgattta gcaatgtatt acaatgaagc
    13201 gggtgctgat gagttagtat ttttagacat ctctaagacg gaagagggtc atagcttaat
    13261 gctagaagtg attgaacaga cagcgtcacg cttgtttatc cctcttactg tagggggtgg
    13321 gattcaaagc cttgatgata ttacccaatt gttaaatcat ggtgcagata aagtatcatt
    13381 aaattcaagt gctttaaaaa atccacagct aattaaacaa gcgagtgata aattcggtag
    13441 acaatgcatc tgcatagcaa ttgatagcta ttatgatcct gaaagaaaag cacattattg
    13501 ttgtacgcat ggtggtaaaa aaatgacaaa tattaaagta tatgactggg tacagcaagt
    13561 agaacagtta ggtgcaggtg agctcctcgt tacaagtatg gggcatgatg gtatgaaaca
    13621 aggatttgat attgagcatc ttgcaaaaat aaaatccctt gtaaatattc caatcattgc
    13681 ttctggtggt ggtggcaatg cacaacactt tgtagaatta tttgatcaga cggatgtttc
    13741 tgcaggttta gctgcaagta tattacatga ccgagaaacg acggttcaat ctattaaaga
    13801 agtgatacgg caagggggta tagcagtaag atgaccaatt acaaaattga ttttagcaaa
    13861 ggtttagtgc cagcaatttt acaagataat caaacaaaac aagtattgat gttaggttat
    13921 atgaatcaag aagcttttga taaaacgata gaagatggtg tggtatgttt ctattcgcgg
    13981 tcgaaacaac ggctatggac aaaaggtgaa acatctggtc atacgcaacg tgttaaagat
    14041 atccatgtag attgcgacaa tgacactatt ttaattgatg tcataccaaa tggaccaaca
    14101 tgtcatacag gcagtcaaag ttgtttcaac acagaagttc cattttcagt gcaaacatta
    14161 gcgcagacag ttcaagatag tgcccaatcc aataatgaaa agtcatatac aaaatattta
    14221 ttaacagaag gtatagaaaa gattacaaaa aaatacggtg aagaagcttt tgaagtcgta
    14281 atcgaagcaa ttaaaggtga caaaaaagca tttgtaagtg aagtagcaga tgaactttat
    14341 catttatttg tcttgatgca tgcgctaggc gttgattttt cagaaattga ggcagaatta
    14401 gcacgtagac atcataagcg caataacttt aaaggcgaac gacaaaatat cgaacagtgg
    14461 taaagcaagt attaactgag atataaggaa aaggatcatg gcatatacac ttacaaatat
    14521 tgtggaaaac gtgacatttt caagtttaaa atacgacacc aacatatttt aactatgaat
    14581 gctgtgatgg tactaaagtt gcgaactcgt tataaataag tagtggataa tcacaatacg
    14641 aaaccaaaaa taatttgaaa agtgaattga gtaactcagg aatagatgtc actgttaaag
    14701 atgttgaaaa gtatatgaat cgatataatg aagttatgaa gggaaaaaat ggcgaaaaag
    14761 ctaaaaagtt atgtttgtcg ttactaccta ttaatatcat agttgtcttt atattctttg
    14821 tatttatact gtaaataaaa atatatctag cctgaaatag aaatgtcata gcctatttaa
    14881 aagacaatct ccattagaac taagatgtgc atcccgaaag ttggactaaa aaactaactt
    14941 tatggggtgt atttttatgc taatcatcat aaattcgaga ttaagttaaa ggtagttcaa
    15001 gagtaattaa acaataaatt aaaaatagta ggatacttat tttgagggaa gaaaattaac
    15061 tgtatatata atgtaggaac aagcgtaatg gcttatccta atacaatcat tgtggatggg
    15121 atgatatttt aaggtttaaa atacgacacc agcaaacatt ataactgtga tagatcataa
    15181 atccccccat acagctaatc taaaaaaata atacatcatt ggaatcaagc ctcaagcatg
    15241 taaatattaa aaatcacaaa agatatatgt aaaaaagtta caatttgcat aattaaattg
    15301 tgtctaatta ttgactaatt aaattttgcc aaatataata ttaattaata atttgaaatg
    15361 attagcgtat acactttaaa ttctctttgg agaatatatt ttttaaatac aaatgtaaac
    15421 gctttctaat caaattaaac aatagaaagg atggtcatta tgagtgcttg gttaagtaaa
    15481 ttatttgagt ttattcctcg aataattatc aatttgttta tctaaaataa aaaaaataga
    15541 ggtgctgaca atgatgaaaa gtcaaaataa gtatagtatt cgtaaattta gtgtaggtgc
    15601 atcttccatt ttaatagcta cattactatt tttaagtggt ggacaagcac aagcagctga
    15661 gaagcaagtg aatatgggaa attcacagga ggatacagtt acagcacaat ctattgggga
    15721 tcaacaaact agggaaaatg ctaattatca acgtgaaaac ggtgttgacg aacagcaaca
    15781 tactgaaaat ttaactaaga acttgcataa tgataaaaca atatcagaag aaaatcatcg
    15841 taaaacagat gatttgaata aagatcaact aaaggatgat aaaaaatcat cgcttaataa
    15901 taaaaatatt caacgtgata caacaaaaaa taacaatgct aatcctaggg atgtaaatca
    15961 agggttagaa caggctatta atgatggcaa acaaagtaaa gtggcgtcac agcaacagtc
    16021 aaaagaggca gataatagtc aagactcaaa cgctaataac aatctacctt cacaaagtcg
    16081 aacaaaggta gcaccatcat taaataagtc agatcaaaca agtcaacgag aaattgttaa
    16141 tgagacagaa atagagaaag tacaaccgca acaaaagaat caatcgaatg ataaaatgac
    16201 cagccatcat tttaacaatg aacaagaagt gaaccctcaa aaagatgaaa aaacactttc
    16261 tgtttcagat ttaaaaaaca atcaaaaatc accagttgaa ccaacaaaga acaatgacaa
    16321 gaaaaaagga ttgaatttat taaaaagtag tgcagtagca acgttaccta acaaaggaac
    16381 aaaagaactt actgcaaaag caacaggtga tcaaacaaat aaagttgcca aacaagggca
    16441 gtataaaaat caagacccta tcgttttagt tcatggtttc aatggtttta cagatgatat
    16501 taatccttca gtgttagctc attattgggg cggtaataaa atgaacattc gccaagattt
    16561 agaagaaaat ggttacaaag cttatgaagc aagtataagt gcttttggaa gtaactatga
    16621 ccgcgcagtt gaactttatt attatatcaa aggcggtcgt gtagattatg gtgcagcaca
    16681 tgcagcaaaa tatggtcatg aacgttatgg aaaaacatac gaaggaattt acaaggactg
    16741 gaaaccagga cagaaggtac accttgttgg acatagtatg ggtggtcaaa cgatacgtca
    16801 actagaagaa ttactgcgta atggtagtcg tgaagaaata gagtatcaaa agaaacatgg
    16861 tggcgaaatt tctccactat tcaaaggtaa taatgacaat atgatttcat caattactac
    16921 tttaggaacg ccacataatg gaacgcatgc ttcagattta gctggtaatg aagctttagt
    16981 gagacaaatt gtatttgata tcggtaaaat gtttggtaat aaaaactcta gagtagactt
    17041 cgggttggct caatggggtc taaaacagaa gccaaatgaa tcatacattg attatgtcaa
    17101 acgcgttaaa caatctaatt tatggaaatc aaaagataat ggattttacg atttgacgcg
    17161 tgagggtgca acagatttaa atcgtaaaac gtcgttgaac cctaacattg tgtataaaac
    17221 atacactggt gaagcaacgc acaaagcatt aaatagtgat agacaaaaag cagacttaaa
    17281 tatgtttttc ccatttgtga ttactggtaa cttaatcggt aaagctactg aaaaagaatg
    17341 gcgagaaaac gatggtttag tatccgttat ttcttctcaa catccattta atcaagctta
    17401 tacaaatgcg acggataaaa ttcaaaaagg catttggcaa gtaacgccta caaaacatga
    17461 ttgggatcat gttgattttg tcggacaaga tagttctgat acagtgcgca caagagaaga
    17521 attacaagat ttttggcatc atttagcaga cgatttagtg aaaactgaaa aggtgactga
    17581 tactaagcaa gcataattta taaagtaaag ggaggaatta ataatgactg cagacttctt
    17641 tcaattaatc ggatcattat ttagaattct aaaagagtta ttcaagtaat accttggcgg
    17701 ggcccccaat acagagaatt tcgaaaagaa attctacagg caatgcaagt tggcggggcc
    17761 ccaacaaaga gaaattggat tcccaatttc tacagacaat gtaagttggc ggggccccaa
    17821 caaagagaaa ttggattctc aatttctaca gacaatgcaa gttggggtgg gacgacgaaa
    17881 taaattttgc gaaaatatca tttctgtccc actcctattg gcatttacgt agtttaaatg
    17941 tgcaataaga atatatgtat aacaatatta aacacgcggt aaaacgaagt cagtcaattc
    18001 aaactgattt cgcccaccgc gtgtttttaa catagcttaa taattaataa gcattaatgt
    18061 tcaatttata tggttgtttt ccaataataa acctaaagat atagaattcg cgcaatatca
    18121 taccgacacc gatacataat cctaaaatga gtaatagaga tattgctaga aagaccatgg
    18181 tattatcttc gaaaatatta gtatatgcaa acaatgagtc tagaatgatt ggatgtaata
    18241 aataaataaa gaatgagaaa gcactaatca tttgaatcgt attaaataac attgttttaa
    18301 aatgcgtgca aatacccaag ataacaataa acataatact attatatggt gttaatgaat
    18361 atgaaaagct ggtaacgttc caatagtctc catttgctaa cgcaataaac acaaaataag
    18421 tagctacagc taatacaatc ataataacta aataacgttc taagaaattt aaaacacgtt
    18481 cgtagttata acccatatat gcacctaaaa agaaataaaa aatccatccg aatattatag
    18541 tgttttcact taatggataa tagtgtagca cggtatcgtg aaacgctgtg ttgttcgtaa
    18601 agtaatataa aaatgattgc tgtaaaataa aagataacaa taataatatt ttactgttga
    18661 ataggttata gttaatttta aaaatgatat aactcaaaat aaagaattgc atgataacaa
    18721 cgataaaata gccataccat tgacctaata ggacattttc aataaattgt ttttgaaaac
    18781 ttgtgtcagt taataatgat tcactataac tgtaaaacaa acccattaat atgtaaggaa
    18841 tcagtatata ttttacgcgt gtagttaagt atctataggt gactttttgg taattcaagg
    18901 ttgtcagtaa ctgtgacaag ataataaagc aaggtgtacc aaaaatgaca atattacgaa
    18961 tatagaactg tagtactaaa gatcctcctt ccatattctc atgttttaaa gtaatttgtg
    19021 ttaataaatg tgtaacaata ataattgcac atataatggc acgtaaatat acgagctcaa
    19081 gtctaatctt tttcatggaa tccgtcccat ctcttaatta aatgctcaaa agcatcatca
    19141 ctaattaata ttctagggat gtaataatca ttggagttcg gagtgactgc tttttcctct
    19201 aatgaaaaac cgtattttaa cccagctttt ttgattaccg gtaatttatc gtcattcatc
    19261 aagccataag gataggctat agttttctgc gactttttaa agtttttagt tagatatttt
    19321 tcacttttgt ttaaatcttt tatgattgta gcttcagaag atttcattaa ttttgactta
    19381 ttatttttag ataagttatg caaatcgtgg gtatgtgttt caaattccca taacccagtt
    19441 ttatacattt cttttagttc ttttttacta atcatatcga gattgtgaaa gttttcttcc
    19501 ccaacatgac ctgtgataat aaacccagtt gccggtattt tatatttttt taagattgga
    19561 taagcatttt cataaatagt ttcatccata tcatcaaagt taatccatac acttcgtttt
    19621 ggaaacttac cttttttctt gtaatataaa aattctttca aggttaaaaa tttagcatca
    19681 tgtgatttta gccattttat ttgagattca aattgtgatt gactaacact ataattttta
    19741 atttctttac tactagaaaa gaagtaaatg aaattattca gaaaattcgc ttttcttaca
    19801 cggtgataat ttaatgccag agcactattt tctttatatt tcagtttttt aggtggatcg
    19861 tcatctgcat ttgcaatatg atgaccatcc agtgtgctta caggcaatat gatcaagata
    19921 ctcaacacta aaattattaa ttttctatac ttcacgattc tcttcctctc tgccattttt
    19981 gaatcaatat gctaattgta aaaaatacaa aaatgataat cgcgaaaatg cccatagttt
    20041 caaatatatc taaaatttca gtattttcaa tgtttaaagc aacacgtatt gtattgatac
    20101 tttcgtcatg aatttcaaat atagtaccaa tataaacgag tagaacaact aaacaatata
    20161 tccaaaagac acacgatata gcgataagtg ctgtttctct tacaatgttt agcgatgatt
    20221 ttagcgttgg gtattccctc tgtctgggct tgaccatgtt gcgtaaccac ctttcttacg
    20281 ttttaatgct tttggaaatg cgacaagaac tactgctgcg ttaataatcc agtatactgt
    20341 cggataccag cttacaaata tgagtccagc catattcttt ttctcgtagc gactatcaat
    20401 aaagagtgcg actgtaaatt gaataacgtt tataaaagtc atagtaaatg atgatagtag
    20461 aaatattgaa aaactatatg tcataaatgt atagtctaag aagtttgctg ttatgaacaa
    20521 atagcctaaa tatagaagca ctatatatac ccataaaatt gagatgattt gctcaaacat
    20581 caaaatatat aaaggaaacc tttttgtttt cattgtgcta aaaaagtctc gtagtaatac
    20641 ttcgtgtccc ccttgagccc atctcacgcg ttgcttccaa agacctccca acgtttctgg
    20701 aaccaacatc caacacatgg caagcggttc atacttaata cgatatccac gtaaatgcaa
    20761 tttccaagaa actgcaatat cttcggtaat catatcagta tcccagtagc caacgtcgac
    20821 aactgcactt tttttaaata gagtgaagac acccgaaata gtattgactg cgccagcaag
    20881 tgtctgactt cgcttaatac agccaattaa acttgcatat tctatcgttt gaattttacc
    20941 taaaatagaa ctcttatttc gaattctagg attacctgta actgcaccaa gttttggatt
    21001 atgtttgaaa ttttcaatca tataatatgg tgcatcttga tcaacgatag tatctgcatc
    21061 caagcacatt acataatcat atgaagcctg tttaatgcct tgattgagtg cgttggcttt
    21121 acctctgttt tcttgtaaat cgacaaaaat aaagtcatta ttttctttaa ttttatagat
    21181 gagttctgct gtattatctg aacttccatc attaataata ataatttctt ttttctcgta
    21241 tttgagtgca agaacattag acaacgtatc ttcaatcgtt tcactttcgt tataacaggc
    21301 aagtaaaaat gtaatgcctt ctaattcatc cacatttatg tcaggcttct tgttcaatga
    21361 atatctaatt tctctagtaa aatagaaata aattgaaccg acaatccagt aaatagacat
    21421 aaatacagga taaaaaagca aaaagttaaa aaattgcaat tcctttacct acctttcgtt
    21481 agttaggttg taagccatat ggtaattgat agtattttaa tttgcaatag attgttgtta
    21541 taattaaacg gaaatatttg taattgcaac ttaattttcc tgtaacatag tgtgattaat
    21601 tttcagtagg gggttttaaa aattgaagga taagattatt gataacgcaa taaccttatt
    21661 ttcagagaag gggtatgacg gtacaacact tgatgatata gctaaaagtg taaatataaa
    21721 aaaagcgagt ttatattacc attttgactc gaaaaaaagt atttacgaac aaagtgttaa
    21781 atgctgtttt gattacctta ataatattat tatgatgaat caaaataaat cgaactattc
    21841 aattgatgct ttatatcaat tcttatttga gtttattttc gacatcgaag aaaggtatat
    21901 tagaatgtac gttcaattat ctaatacgcc tgaggaattt tctggaaata tttacggaca
    21961 aatacaagat ttaaatcaat cgttaagtaa agagatagca aaattctatg atgaatcaaa
    22021 gataaagatg acaaaagaag actttcagaa tttaatattg ctgtttcttg aaagttggta
    22081 tttgaaagca tccttttcgc aaaaatttgg agcagtggaa gaaagcaaaa gtcaattcaa
    22141 agatgaagtg tattcgctac taaatatatt tttgaagaaa taatttttgt tactagtttg
    22201 taataattaa cttacttttg taaaaaaaga catgagatta tttttttaaa tctatataaa
    22261 gttgacaata caactcgaaa ttgagaatat taagatgtat atgaatttta taaattaaat
    22321 acaatacatt aatataaata tcaattgttg caaaatacga tttgttcaat gatttgataa
    22381 tattattctt tatatttgtg aatggttaag tttgtctttg aacatattat aaaagtgtaa
    22441 tgttcccctg aaaagaataa gttgtcatct aattacagga aatccgcata aattagatga
    22501 aatggaaagt aataattaat aatttattga taagcgccta tgtgatggta aatcatgaca
    22561 taggcgcttt tttttataag tttaaaatgt aaataaaaat tatataaatt acccacatct
    22621 ttttaaaaga tgtgggcttt atcatcatta acccaactca cagtgacggg ttacgcaaag
    22681 tattgaatta ccgagtacgg gcacgctcgg tgttgtaaag agcaaataat caagtaatga
    22741 tgatgcttct actcgattat aagaaagcca tgatagagac gatggtatct aggtttatta
    22801 ctgataggtt tggatatttg aagttggaca atattatatc tcgaacgaaa atataaataa
    22861 attatacata acgaccaaga atcatgatat aattttaaac gataaaatat ttatataaaa
    22921 aattagagaa aatgtagttg tgtatttttg tggtcgttaa actagatata attgtccgat
    22981 ttataaaaca tacataatga atacaatgat tgattatgtg gaggaaacca tgaaagaaaa
    23041 gtttgattta gtaaaactat taaatattct aaagaagaat attaaattat tgcttatttt
    23101 accggcaata tgtcttgtag taagtgcggc attaaccttt tttgttatgc ctgacaaata
    23161 tactgcttct actcaaatat tagtaaacat gaaaaagtcc tcaagtgatt tagctttcca
    23221 aaatgttcaa agtagtttgc agtctgttaa tacatatacg gaaattatca aaagtccaag
    23281 aattctagat aaagtatcta gagagtttga tggtcagtat tcaacagctg agttgaattc
    23341 gtttttaaaa gtaaccaatc aaacaaactc tcaaattatt actgtatcag ttacaactgg
    23401 gaataaatct gaatctgaca aaattgttaa tagaatatct aaagtttttg cacatgacat
    23461 gcctaaaatt atgagtgttg ataatgttac aatactttcc tcagcacatg ataatgctgt
    23521 aaaagtatct ccaatagtat ctgttaactt agtaatcagt atcattgttg gtattgtttt
    23581 agcaatattg attattttct taaaagaatt attagataag cgtattaaga cggaagaaga
    23641 tgttgaatcg caactaggat tacctatttt aggttcaata caaaaatttt aatttacgag
    23701 gaattaccat gacgaataca cgaagaagta catcaagttt aattgtccat gaacaaccaa
    23761 agtcacctat tagcgagaaa tttcgaggca taagatcaaa tattatgttt gcaaatcctg
    23821 acagtgcagt tcaaagcatt gtaatcactt cagaggcacc aggcgcaggt aagtctacaa
    23881 ttgcagcaaa tttagcagtt gcatatgcac aagcaggata taaaacatta atcgtagacg
    23941 gggatatgcg taaacctacg cagcactata tttttaattt gccaaataat gaaggcctat
    24001 caagtttatt gctaaattgg tcaacttatc aagatagtat tatctcaact gaaattgaag
    24061 atttagacgt cctgacgtct ggaccgattc cgccaaaccc atcagagtta atcacatcaa
    24121 gagcatttgc gaatttgtat gacacattat tgatgaatta taactttgta attatcgata
    24181 cgccaccagt gaacacagtt acagatgcgc aattattttc aaagtttacc ggcaatgttg
    24241 tctacgtagt taattcggaa aataataata aagatgaagt taaaaaagga aaagaactta
    24301 ttgaagcaac aggtgctaaa ttattaggtg tagtcttaaa tagaatgcct aaagataaaa
    24361 gtgctagtta ctatgcatat tatgggactg atgaatcatg attgatattc ataaccatat
    24421 attagttgat atagatgatg gtcctaagac aattgaaaag agtattgcac tattaaagca
    24481 agctaaggat gaaggtgtaa caagtattgt agcgacacct catcatttgc atccgagata
    24541 tgataataca tttcaacaag tgcttgtgaa attggcggag ttaagaacgc atccagaagt
    24601 tcaagcattg gatattaaat tatttcccgg gcaagaaatt agaattacag attcgattct
    24661 gcaaggctta gataatggta gtattcaagg tattaatcgt tcgaaatatt tactaattga
    24721 atttccaacg ggcgaagtac cacattatac aaaacaattg ttttttgaaa tacaatcgag
    24781 aggctacata ccaatcattg cacatcctga aagaaataga agtatcgcca aaaatccaga
    24841 aatattatat gaacttgttg caaatggggc attgagtcaa ttaacatcga gttctttagt
    24901 tggtggattt ggaaaaaaca ttcaaaagtt atcattacaa tttatagaat gtaatttggc
    24961 tcattttgta gcttcagatg cacattcatg tgaccaaaga ccatttttga tgcaagaatt
    25021 atttcacaat cataaattga agaaatattc gaatgacatt gaagcattac ttcgaaatgc
    25081 cagttcagta atcaatgata attttgttta tctagacaga ccgactaagc caggaaaagt
    25141 gaagtcgttt ttgaaatggt tttaactaaa tatatttttt cgaattagac tggtatacat
    25201 gtaccagtct ttttgagtat gcttaaaatg aattataatt atgattattt gctaattcgt
    25261 ataatacaac ttgtctaatt gattaaggcg aatggctttt aataaaaaga ctcgtttttt
    25321 atagtgaagg tagtttgaaa ataatgaata tatagatgaa ggtattactt ttgatatata
    25381 gggctaagga agtgttttta tggtattaag aaaagtaatc atgcaggact tagatcaaat
    25441 tattgcactt gaaaatattg gtttttcacc ggaagaagct gcaacaccag aggcattgaa
    25501 attgagaatt gaacaaattc aagaaacttt tattgtagct gaaaagaatg atgaagtgat
    25561 tggatatatc aacggtcctg tcataaaaga gcgttacatt agcgatgatt tatttaaaaa
    25621 tgtccccgca aataatagtg aaggtggtta tattagtgtg ttaggtcttg ttgtagcacc
    25681 aaattatcaa ggtcagggta ttgcaggtcg cttactaaac tattttgaaa atcttgctaa
    25741 gaatcaacat cgacatggtg taacgttaac atgtcgagaa tcattaattt ctttttatga
    25801 aaaatatggt tatcgtaatg aaggtgtatc agaatcatgt catggcggta ttaaatggta
    25861 taaccttgtt aaaaatataa aaaaggatga tgaggtgtaa atatggcagt tgtttatgta
    25921 gcaggagggt gtttatgggg tgttgaagca tttttcgcaa caatacctgg aattatacat
    25981 acagaagcag gaagagcaaa tggaagaagc tctaaattag acagtccgta tgatggttat
    26041 gctgaatgtg tcaaacttca ttttgatgat cgtatgttaa caattacaga catcatgaat
    26101 tatttatttg aaatcattga tccgtacagt gtgaatcgac aaggaaatga tattggagaa
    26161 aagtatcgaa cgggtttata tagttgtgta gatgaccact taattgaagc gcatcagttc
    26221 attgaacgac gtaaagacag agataaaatt gcagtggaag tcttaccgct gtctaactac
    26281 attaaaagtg ctgaagaaca tcagcaacat ttagaaaaat atccagaaga tatgcatatg
    26341 tgtcatattt caatagattt gctaaataaa tacaagtgat tttcagagga gaatagatgt
    26401 ttaaattaac caaaatagga gaaataccta gatatactaa gaaaagaggt aggtattatg
    26461 catacgtttg atgcacaatc attaactgct agagaaaact ataaattatt aattggttca
    26521 attattccac gacctatagc ttttgttaca acattaaatc aagatacatc agtaaatgca
    26581 gcaccattta gcttttttaa tattgttaat aatcatccgc caatgattgc tattgcagtt
    26641 caacgtgtag gaggtaagag aaaagatact gccttgaata tagagcggac tggagacttt
    26701 gttgtgcata ttacagacga agataatgtt caagatatta atgagactgc agcgccgtta
    26761 gcatatggtg acagtgagtt gagccgtaca gaattatcac ttttaacatc tacaacgatt
    26821 aagacgccag gaattaaaga cgcgaaaatg agattcgaat gtaaactatc gcaaatgata
    26881 ttgcttggag atgtattaga tggggctgac ttaataatag gtgaaattgt aacttatcat
    26941 attgatgaca gtatttatga gggtgatttt aaaatcaatc cacacgcatt acaggctgtt
    27001 tctcgtttag ctggaaatga ttatgctaaa ttaggaggat tatttacaat agatagacct
    27061 gaaaaataac aaaacacatg ctatagcggt gtaccgttta tggattgaat caaaagtaca
    27121 caggtgctaa agtatgtgct tctaaaatga agactatttt ttattacaag aaaatgtatc
    27181 tagtaaactt aaagtagcaa gacctaataa atctaatgca tgttgtgcac cttttttacc
    27241 ttggccagct tcgaaatgtt tataagcagc tacacttaaa atacctattg ttgacagtga
    27301 agcaaggcga gaaatgtttt tattgataaa gctagctgag tataaagcag cagtagttgc
    27361 ttctgcaatg ccgacgtatt ttacaagttc tttttgcaag ccaaaagtat gttcaaacag
    27421 ttcaatcata cccttatctt cttgcaattt aggtttactg gcttgatata gctctttcgc
    27481 aagttttaaa ttggtcacgt aacgtaaaat catattttta ttcctcccaa tatttgattt
    27541 tttgaaaaac ggttgtttta tcattttacc cgtttcttca aaaatgaatc tcttatgtaa
    27601 catgtgtagt tcaggaatgt ttgctatgga aatataattc tattcactca aaatatatga
    27661 atttaatgtg tggttttatc gagttgctct tttaatttgg ttagattgtt ttttagagaa
    27721 gcggtactat tttaaagtgc atcaacagat ttaccttcgt tttgagacat tgcgtttata
    27781 acagcatgaa gttccgtttc taacatgtcg gcgtcacctt tagcattaga acttaatact
    27841 ttaatatctc ttaaaaggct atctgatagt agggcatttt tgaaaaagtc ttgcatctca
    27901 ataataacaa attctgtaaa attagttcac caaggattaa tcgcatatca gttaattgtt
    27961 gatgtaattt atcaacaaga tttttgaaag atttaagtgt ttccgctgtt gtcttggcaa
    28021 atggaacttt ggaggcaatc cattcaatct taccaacaaa attagataat gttggcgtgc
    28081 atgcatcaac aaacgtaatg atataaaaga gttttgatgt aattggtact aataaaatat
    28141 ttatacttga cgcaagtgaa gccaaaccag cttccatcac tttatttcga atttctaaat
    28201 ttaataataa attcttagat tctgcaagtg gttttgatgt ttgcttcgta gcatttttat
    28261 tgatgggtac gttgttaacc attacattgt catccaaatg ggtcatttgg gtcaatatat
    28321 cattaatgcc atcaccgaaa ttagtgactt gacttttgat aatttgaata tttggtatga
    28381 caacattcaa atgggctaaa acctcttctc taacaccatc ctcaaatcct tctgataagt
    28441 tgacaaacat tttgacaata gtgttgctaa ctatatcaaa agcagcttcc atgaaatcat
    28501 caccatattt aatgaatttt gaaattttat cggtaatttt atcaataaaa tcaataattg
    28561 aattaataat atgatcggca cctactaaat ctaaaatatt atcttggtct ctaagtgttc
    28621 tgaggtcacc ttttaattca tccgctttac tttcaatttt agtcctaatc agattggata
    28681 tgttgtgaat ccaaccttct aattcgttaa gttttattac ttttttaaaa tgatctttcg
    28741 tttcatttac atgttgacta aaattattat tttcacgttc aacggatgtt cgaatgtctt
    28801 tcattttaat atcaatatta tttacatatt cggttaatct accatgcaca tagtcattca
    28861 gatccgccag atgtcctttg tctaatttta tttttgatgt accagcacca atagcatcac
    28921 cactccatac aaatattgga gtgtgttcat ccatgtcagc ataaattttc aatccattgc
    28981 gactcgtgta gacttccttg ttaataatat tgttttgatc aaaatggtct aacttctctt
    29041 tttcttcaaa gttgtatcca gtatgagcag gtaccaatgc tccaaacgta gacacaccat
    29101 tttctaggaa aatgtgcttt ccgaccatgg aatgatacat aagtccacct atgttaattc
    29161 tagttaaggt atcacttcta ctgtggtaat tatgtatatt atttgcatct cgacgagcca
    29221 tacgggtaag agattttggt ataggtgcag aatttgtagt agttacgcta agacttggat
    29281 ctaacacacc taaatactgg gctatggctc cagagagtga attcccccct gcatttgtga
    29341 ttgattttcc tttgtaatca atagactctt ctttatattt attataaaat tcaatatcac
    29401 ttaaagaact tgcatcactg tatttttgtt ttaaatcaga atacactttg tgtgcttctt
    29461 ccaattgatt tggaatggag tctccagcga gtcttaaatc tgtacctaca tcgtttaggt
    29521 cactaaagtc agtaccatca aatgatatga ttaactttcc tgtacttcta tcattatcat
    29581 aacgttcata taacttataa ccgagacctg tattagtcgg tttttgatct ttaataactt
    29641 tatatttatt tccattaaca tgaatctcgt ctgacaaggt tattttgtca ttgtaattcc
    29701 acccactact taattcaata gcatctaaat cggttatatg atcatttttt ctcattataa
    29761 ttgcccccga taacatatat ttcatctgca tcaggattta agcgcccata ttgatcaagt
    29821 tcataaggtg atactttatc tttgtaaaat ctcatacgtt tttcacctgg gtatgttttc
    29881 ataatttcgt tagtgggttt ttttagactc caagcataat cagctacagt ttctttattt
    29941 gcatctttat cgtaataggc aataaactcg gacttcatat ttttttgtat ttgtggatat
    30001 ttattaatta aattttgtaa ttgttgattg aatgcatctc cttttaagtg catgacaggc
    30061 ttgaattcct ttttaaactt atctaggtca tctatactaa cggtattaaa atttaaatat
    30121 tgtttagtaa caccattgaa ttcttgtttc ttttgaagtt tttcattcat ggcataataa
    30181 ccgtttttct caacttctga tttaaatttt aaccgagctt gttctatatc gtgttcataa
    30241 tgtttataca tcatggctgc aactgcggct gacgtttcgt ttttatggcc aggtgtaaat
    30301 gttgtgatag ttatgccatt gtcagattca acaaaaatca gtctaaactg aaaaggaata
    30361 tcataattga ccgtaccaac gactactaag ccatctgttt tacttttctc actaaaccca
    30421 gatttattag tataggcctc aactaaatta actttacaat cataaccaaa ggtgtctttc
    30481 acatagtctt tgtatttttt gattgcttca tctttatgtt ttttcagaaa tacttcattt
    30541 ctttttttat gttcaccaat atatttacca tcataatctt tgacgctaac cgtctttggg
    30601 atatcttttg tgtgatcttt atgcataaaa gaacagcctc ctaaaataag tgatgtacac
    30661 aatacaaagc cagttacttt tctcattttt actatctcct ctatattgag tatttttata
    30721 acgaaaataa aaatataaat aatagttaaa gtgttccaat ccaatataat tataacttta
    30781 ttattatagc taataatttt atatttaatt gttaaagtta ttgtaattat ttggataagg
    30841 aagtatatta cactaaagtg ttttcaaggt ttttaattgt tttcccctcc agaaatggag
    30901 atttcttgag aatctgattg taaacgttta tgctcatcta attgtgtagc agaaacttta
    30961 tctttataaa atatgatgct tttatttcct ggataaagtt tcattgtgtc attagttgtt
    31021 ttttgtaggt tccaaacata gttagcaaca gtctctctgt tctcaccttt atcgtaataa
    31081 gcaataaatt ctgactccat atttttttgt atttgaggat atttattaat caaagattgt
    31141 aattgttgat tgaatgcatc tccttttaag tgcataacag acttaaattc ttgtttaaac
    31201 ttttttaagt catcaatact gtctgtgttg aaattcaaaa attgtttagt aacaccattg
    31261 aattcttgtt tcttttgaag tttttcattc atggcataat aaccattttt ctcaacttct
    31321 gatttgaatt ttaatcgagc ttgttcaatt tcatgttcat aacgtttgta catcatggct
    31381 gaaacggccg ctgatgtttc atttatatga ccaggtgtaa atgttgtgat agctatgcca
    31441 tttccagact caacaaatat cagtctaaac tgaaaaggaa cgtcataatt gacagtgccg
    31501 acgactacta aaccatctat cttacttttc tcgctaaacc cagatttatt agtataggcc
    31561 tcaactaaat taattttaca atcataacca aaggtgtctt tcacgtagtc tttgtatttt
    31621 ttgattgctt catctttatg ttttttcaga aatacttcat ttcttttttt atgttcacca
    31681 atatattgac catcataatc tttgacgcta accgtctttg gaatatcttt tgtttggtac
    31741 tttggtatga aagagcaacc gcttactatg attgatgtgc ataataagat ttgcataatt
    31801 tttctcatat cttcattagc gcctttgtat tagctgtttt tcagttgata ggcaaattat
    31861 acaaaaacta aaataatgtt attcaatatt attataagat aatcttgatt atattaacta
    31921 atattaatta aattaacaaa aaacatcatt aaatattaat tgcataaatc cttatctaaa
    31981 attttcgaat caagaacaca aattataagc aaccacgtca tatatagttt ctacttataa
    32041 ataaattgat tatttatatt agacgcttat gtcatgagag cgtaacctta aatgtaatta
    32101 ctaataaggg gtgaggatag aatgaattat tcgccgaagc aaggtgtaaa gagtcatggc
    32161 ttaccacacg atccatttaa aagtagtaca gtaccacgtc caataggttg gatctctact
    32221 gtatcgaaag atgggaaaga taatttagca ccttatagtc agtatcaaaa cttaacttgg
    32281 gatccgccta tggttatgtt cgcagcaaat caatctgtgc ttggtgatca tgagcgtaaa
    32341 gatacagtaa aaaatgcaga agagacaggg tggtttgtat ggaacatggc cacgtatgat
    32401 ttaagagaag ctgttaattt atcgtcgaag gcactaccac ctgaagagga tgaatttgaa
    32461 tatgcaggtg tgacgaaaga agcatgtata gaagcaccag gttttcgagt caaagaatcg
    32521 ccagtacatt ttgaatgtga gtacgttcaa acgatacgta ttccgacagg agatcctgta
    32581 tcaacagttg atattgttat aggaagagtt gctcaggttc atattgacga taaggtcatt
    32641 cttgataatg gtaaattaga cattaagtcg attagaccta ttgctaggtt aggatattat
    32701 gattacacgg ttgtgaatga aatatttgaa atgaaagcac catcagcttc taaagaagag
    32761 cttgcaggtt tagaaggaag aaattttgat aatcaatcag atgacaagga ataataaatt
    32821 caatcaattt caatacatcg aaactacaca ttgaaattag ttttgatgta tttttatatt
    32881 tcaaaaccgg gaagcgatat gtcgctatat ttataatttt aaagaagaat tttttagaaa
    32941 ttcagatata taacactagg tttattgaaa atgtattgat attactttaa tattaaagat
    33001 gcattataaa tataatattt atcttttagt tgtaaattga ctattaatga ataaaaggtg
    33061 gatggtaaat acattttaat aaaaagacat ttttactaaa taagtaaaag ttatctaaat
    33121 tgataaacat tgatttcaaa attatataaa cataataaga catgttattc agaatatagt
    33181 taaaaaatta tactaaatcc cacacaataa agaaagcgat ttcatcaaag aatatacttt
    33241 tgagaaaaaa tggattttga caaattaagt attttttatt tgaaaaaaga gttgtattaa
    33301 attgaatagt ggcatatatt ataatctaat gaatgtaaag ataatacaag gagttattac
    33361 atgagcaaaa gacagaaaga atttcatgac agcttagcaa acgaaaaaac aagagtaaga
    33421 ctttataaat ctggaaaaaa ttgggtgaaa tccggtatta aagaaataga aatgttcaaa
    33481 ataatgggac taccatttat tagtcatagt atagtgagtc aagataatca aagcattagt
    33541 aaaaaaatga ctggatacgg actgaaaacg acggcagtga tcggcggggc atttacaata
    33601 aatatgttac atgatcagca agcttttgcg gcttctgatg cacctttaac atctgaatta
    33661 aacacgcaaa gtgaaaccgt aggcaatcaa aactcaacga caatcgaagc atcaacatca
    33721 acagccgatt ccacaagcgt aacgaaaaat agtagttcgg tacaaacatc aaatagtgac
    33781 acagtctcaa gtgaaaagtc tgaaaatgtc acttcgacaa ctaatagtac aagcaatcaa
    33841 caagaaaaat taacatctac atcagaatca acatcctcaa agaatactac atcaagttct
    33901 gatactaaat ctgtagcttc aacttcaagt acagaacaac gaactaatat atcaacaaat
    33961 caaagcactg catcaaatac tacttcacaa agcacagcgc cagctccaac ccatttaaaa
    34021 caaactagca caacgtcaac cagcacagca cctgtcaaac ttcgaacttt cagtcgatta
    34081 gctatgtcaa cttttgcatc aactgcaacg acaagtgcag taaccgctaa tacgattacg
    34141 gttaataaag ataacctcaa acaatatatg acaacgtcag gtaatgctac ttatgatcaa
    34201 agtaccggta ttgtgacgtt aacgcaggat gcatacagcc aaaaaggtgc tattacatta
    34261 ggaacacgta ttgactctaa taaaagtttt catttttctg gaaaagtaaa tttaggtaac
    34321 aaatatgaag ggcatggaaa tggtggagat ggtataggtt ttgccttttc acctggtgta
    34381 ttgggagaaa cagggttaaa cggtgccgca gtaggtatcg gtggcttaag taacgcattt
    34441 ggcttcaaat tggatacgta tcacaataca tctacaccga atgtatcagc aaaggcgaaa
    34501 gctgacccac ctagtgttgc tggtggaggt gcgttcggtg cctttgtaac aacagatagt
    34561 actggtgttg catcaacata tacatcaagt tcaatagctg ataatgctgc aaagttaaat
    34621 attcaaccaa caaataacgc atttcaagat tttgatatta attataatgg tgatacaaaa
    34681 gttatgactg tcacgtatgc aggtcaaaca tggacacgta atatttcaga ttggattgca
    34741 aaaagtggta cgaccaactt ttcattatca atgacagcct caacaggtgg cgcgacaaat
    34801 ttacaacaag tacaatttgg aacattcgaa tatacagagt ctgctgttac acaagtgaga
    34861 tacgttgatg taacaacagg taaagatatt attccaccaa aaacatattc aggaaatgtt
    34921 gatcaagtcg tgacaatcga taatcaacaa tctgcattga ctgctaaagg atataattac
    34981 acatctgtcg atagttcata tgcatcaact tataatgata caaataaaac tgtaaaaatg
    35041 acgaatgctg gacaatcagt gacatattat tttactgatg taaaagcacc aactgtaact
    35101 gtaggcaatc aaaccattga agtgggtaaa acaatgaatc ctatagtgtt gactacaacg
    35161 gataatggta ctgggactgt gacaaataca gttacaggat tgccaagcgg attaagttat
    35221 gatagtgcaa cgaattcaat cattgggaca ccaacaaaaa ttggtcaatc aacagtgaca
    35281 gtagtatcta ctgaccaaac aaataacaaa tcgacgacaa cttttacaat aaatgttgtg
    35341 gatacgacag caccaacagt gacaccaata ggagatcaat catcagaagt gtattcacca
    35401 atatccccga ttaaaattgc tacgcaagat aatagtggga atgcagtaac aaatacagtc
    35461 acaggattgc cgtctggatt aacattcgat agcacaaata atactattag tggtacacca
    35521 acaaacattg gtacaagtac tataacaatc gtttctacag atgcgagcgg taacaaaacg
    35581 acgacaactt ttaaatatga agtaacaaga aatagcatga gtgattccgt atcaacatca
    35641 ggaagtacac aacaatccca aagtgtgtca acaagtaaag ctgactcaca aagtgcatca
    35701 acgagcacat caggatcgat tgtggtatct acatcagcta gtacctcgaa atcgacaagt
    35761 gtaagcctat ctgattctgt gagtgcatct aagtcattaa gcacatctga aagtaatagt
    35821 gtatcaagct caacaagcac aagtttagtg aattcacaaa gtgtatcatc aagcatgtca
    35881 ggttcagtta gtaaatcaac atcattaagc gattctattt cgaactctag cagtactgaa
    35941 aaatccgaaa gtcaatcaac aagcacatct gattcattgc gcacatcaac atcgttaagt
    36001 gattcagtaa gcatgagtac atcaggaagc ttgtctaagt cacaaagctt atcaacaagt
    36061 acatctgatt cggctagtac atcacaatcg gtaagtgata gtacatcaaa ttcaattagt
    36121 acatcagaat cattgagcga gtcagctagc acctcggact ctatcagtat ttcaaatagc
    36181 atagccaact ctcaaagtgc gtcaacaagc aaatcagatt cacaaagtac atcaatatca
    36241 ttaagtacaa gtgattcaaa atcgatgagt acatcagaat cattgagcga ttcgacgagc
    36301 acaagtggtt ctgtttctgg atcactaagc atagcaacat cacaaagtgt ctcaacaagt
    36361 acatcagact caatgagcac ttcagagata gtcagtgact ctatcagtac aagtgggtca
    36421 ttagctgcat cagacagtaa atcaatgtct gtaagtagtt caatgagcac gtctcagtca
    36481 ggtagtacat cagaatcatt aagtgattcg ataagtacct cggattctga cagtaagtca
    36541 ttatcattga gtacaagtca gtctggctca acaagcacat caacatcaac aagtagttca
    36601 gtacgtatgt cggaatcaca aagtacgtct ggttcaatga gcacaagtca atccgattca
    36661 acgagcatat caacgtcgtt tagtgattca acaagtgata gcaaatcagc atcaactgca
    36721 tcgagtgaat caatatcaca aagtgtctca acaagtacat ctggttcagt gagtacttcg
    36781 acatcgttaa gtacaagtaa ttcagagcgt acatcaacat ctatgagtga ttccacaagc
    36841 ttaagtacat cagagtctga ttcaacgagc gactcaacgt cgacgagcga ttctatcagt
    36901 gaagcgatat ctggatcaga gagtacgtct atatcattaa gtgaatcaaa tagtacaagt
    36961 gattcagagt cgaaaagtgc atctgctttt ttaagtgaat cattgagtga aagcacatcc
    37021 gaatcaacat cagagtcatt aagcagttcg acaagtgcga gtacgtcatt atcagacagt
    37081 aattctgaat ctggtagcac atcaacatca ttaagcaatt caacaagtag cagtacatct
    37141 atttcaacgt caacaagtgg tagcgcatca acatcagcgt ataagagcga gagtgtctca
    37201 acatcattga gcacatcaac gagtacaagt ttgtctgact ctacaagttt gtcaacatca
    37261 ttaagtgatt cagcaagtgg tagcaagtcc aattcactaa gtgcatcaat gtcgacaagt
    37321 gattcaatca gtacaagaaa atcagagtca ttaagtacat cgacatcatt aagcggttca
    37381 acgagtgaga gtgaatccgg ctcaacatca tcaagtgaaa gtaaatccga ttcaacatca
    37441 atgagcctga gcatgtccca atcaacatca ggaagtacaa gtgtgtcaac atcagaaagt
    37501 ttgtctgact caacgagtac atcattgtca ctgagtgcct caatgaatca aagtggtata
    37561 gactcaaact cagcaagcca aagtgtctca gcatcaacaa gtacaagcac gagcgaatcc
    37621 gattcacaaa gcacatcatc atatacaagt cagtcaacaa gccaaagtga atcaacatca
    37681 acatcaatgt cactaagcga ttcaacaagt atgtctagaa gtacgagcca atcaggttca
    37741 acaagcacat cagcatcatt aagtggttca gagagtgaat ctgattcaca aagtatatca
    37801 acaagtgcaa gtgaatcaac atcagaaagt acatcaacat cgctcagtga ctcagaaagt
    37861 acaagcaact caacatcaga aagcacatcg aatgcaatta gtacgtcagc aagcgcaagt
    37921 gaatccgatt cgtcgtcaac atcattaagt gattcaacgt cagcgtcaat gcaaagcagt
    37981 gaatccgatt cacaaagcac atcaacatca ttaagtaatt cacaaagcac atcaacatca
    38041 aaccgaatgt cgactattac aagtgagtca gtatcggaaa gtacatcaga atctggctca
    38101 acatcagaaa gtacaagtga atccgattca acatcaacat cattaagcga ttcacaaagc
    38161 acatcaagaa gtacaagtgc atctggatcg gcaagtacat caacatcaac aagtgactct
    38221 cgtagtacat cagcttcaac tagtacttcg atgcgtacaa gtacgctgga ttcacaaagt
    38281 atgtcgcttt caacaagtac atcaacaagt gtgagtgatt caacgtcatt atctgatagt
    38341 gtgagtgatt caacatcaga ctcaacaagt acgagtacat ctggttcgat gagtgcgtct
    38401 atatcgttaa gtgattcgac aagcacatca acatcggcta gtgaagtaat gagcgtaagc
    38461 atatctgatt cacaaagtat gtcagaatct gtaaattatt cagaaagtgt aagtgaatct
    38521 aattctgaaa gtgactcaaa atcgatgagt ggttcaacca gcgtcagtga ttctggctca
    38581 ttgagcgtct caacgtcatt aagaaaatca gaaagtgtaa gcgagtctag ttcattgagt
    38641 ggctcacaat cgatgagtga ttcagtaagc acaagcgatt cgtcatcatt aagtgtatcg
    38701 acgtcactaa gaagttcgga aagcgtgagt gaatctgatt cattaagtga ttcaaaatcg
    38761 acaagtggtt cgacttcaac aagtacatct ggttcattga gcacttcgac atcattaagt
    38821 ggttcagaaa gtgtaagcga gtctagctcg ctaagtgatt caatatcaat gagtgattct
    38881 actagtacaa gtgaatccga ctcattaagt ggatcaatat ctttaagtgg ttccacaagt
    38941 cttagcactt cggattcatt aagtgattca aaatcattaa gtagctcgca aagtatgagt
    39001 ggatcagaat caacgtcaac aagcgtgagt gattctcagt caagctcaac tagtaatagt
    39061 caatttgact ctatgagcat cagtgcatct gaaagtgact cagtgtctac aagtgattca
    39121 tctagcatca gtggatcaaa ttcaacgagt acatcacttt caacatctga ttcaatgagc
    39181 ggaagcgtat cagtttcaac atcgacaagt ttaagtgact caatatcagg ttcaataagt
    39241 gtaagtgact cgagctcaac gagtacatca gagtcgctaa gtgattctat ggcacaaagc
    39301 cagtcaacaa gtacaagtgc atctggttcc ttaagtacat cgatatcaac atcaatgtca
    39361 atgagtgcaa gtacatcgac atcacaaagc acgtcggtgt cgacatcatt atcaacatca
    39421 gacagtatca gtgattccac ttcaataagt atcagtggtt cacaaagtgc tgtagaatca
    39481 gaatctacaa gtgattcaac ttctatcagt gactcagaat cattaagtac atcagattca
    39541 gactcaacat caacaagtac atcggactca acaagtggtt caacttcaac aagcacatct
    39601 gaatcgctaa gtatgtctgg ttcagattca acgagcgtat ctgactcaac atcaatgagt
    39661 gaatccgatt caacaagtgt ttcaatgtca ttgagcacat ccgattcaac atcaattagc
    39721 gactcagaat cagtgtcaac aagcacatca atgtcattga gcacatccga atcgctgagt
    39781 acatctatgt ctggttcaca aagcatttct gactcaacat caacaagtat gtctaactcg
    39841 acttcaatga gcaactcaac atcaacaagt atgtccggct caacaagtac atctgaatct
    39901 aactcaatgc atccgtcaga ctcaatgagt atgcatcata cacacagcac gagcacatct
    39961 cgctcatcaa gtgaagcaac aacgagcacg agtgaatctc agtctacatt aagtgcaaca
    40021 tctgaagtga ctaaacataa tggcacgcca gcacaaagtg aaaaaagatt gccagataca
    40081 ggtgactcaa taaaacaaaa tggattacta ggtggcgtta tgacattatt agttggttta
    40141 ggtttaatga agagaaagaa aaagaaagat gaaaatgatc aagatgattc tcaagcataa
    40201 attgaattta tagttggcta aaattaaaaa gactggggcg ttgttcacta aaaaatctat
    40261 atcagatttt aataattagt gaaataacct tagtcttttt aatgtttttg atataaaaga
    40321 ggtgcaacat gttaaaactt ttacaacaat acgaatataa aattatatat aaacgtatac
    40381 tttacacatg ttttatcttg tttatttata tattaggtac aaacatttcc atcgtttcct
    40441 ataacgatat gcaagtaaaa catgagtcat tttttaaaat agctatttct aatatgggtg
    40501 gagacgttaa tactttaaac attttcactt taggattagg tccgtggtta acatcaatga
    40561 ttatcttgat gctgatttct tatcggaata tggataaata catgaaacaa acaagtcttg
    40621 agaagcatta taaagagcgc atcttaacat taattttaag tgttattcaa agttattttg
    40681 tgattcatga atatgtttcg aaagaacgtg ttcatcaaga caatatttac ttaacaatct
    40741 tgattttggt tactggaacg atgttattag tttggttagc tgacaaaaat agtcgttacg
    40801 gcatcgcagg accaatgccg attgtaatgg ttagcattat taaatcaatg atgcatcaaa
    40861 aaatggaata tatagatgca agtcatattg tgattgcgtt gttaatcact cttgtgataa
    40921 tcacattgtt tatcttatta ttcattgaat tagtagaagt gagaatacca tatatcgatt
    40981 taatgaacgt ttccgcaaca aatatgagat cttatttatc ttggaaagtt aaccctgcag
    41041 gcagtattac tttaatgatg agtatttcag catttgtttt cttgaaaagt ggtattcatt
    41101 ttattttatc tatgtttaat aaagacgttt cagatgatat gccaatgatg acatttgata
    41161 gtccaattgg tatttcggtg tatttagtga ttcaaatgtt attgggttat tttttatcga
    41221 gatttttaat caatactaaa caaaaatcca aagatttctt aaagagtggc aattattttt
    41281 caggagttaa acctggtaag gatacagaac gttatttaaa ttatcaagca cgacgcgtat
    41341 gttggtttgg atcggcatta gttacagtca ttattggtat accactttat ttcacattgt
    41401 ttgtaccgca tttatctact gaaatttatt tctcagtaca actgattgta ttagtttaca
    41461 tcagtattaa tattgcagaa acaattcgta catatttata ttttgataaa tataagccat
    41521 ttttaaacca gtattggtaa ggaggtaatt atgaaatact ttattccagc ttggtacgat
    41581 gaccaacgat ggtggcaaga cacgactgtg ccgtattatc aactacaaaa taatacggaa
    41641 tttgacgata tgattagttt gatggggatg caccttgaaa atgacttaga ttatcgtctg
    41701 attgttctca atcatgcacc aaatttaaga acatttttac atcgatatga cttatatgag
    41761 acaaagtatt ggtctgtgtt tgatgaaatt caaggattca gtcaccatgc gccacaagcg
    41821 attaattatc atcacttaaa atggccggat gatgttgagt ttgtgtacac gccgtattta
    41881 ttaaaatgtg tgacgagtga acagacctat acaaatattt attttagtca agaagggtat
    41941 tcaatttggt ttgaagaatt tgaaagagat cagttacaac ggcgttatat ttttgatgat
    42001 agaggttatt tatcagctat acgttatttt gatgatcagg gagaggcttc ttaccaagaa
    42061 tatttaacga ttaatggaga ttgtgtgctt tttgaaaact ttaaaaatgg acgagtcact
    42121 gtttcaaaaa gatatcaaca tcactatcaa caaacagaat ataacaatat ggctcaacta
    42181 attgaagaaa aatttcaagc aatgattgca caacaaatac atgaagatga tcatgtgatt
    42241 gtggcttcag atgctaggca caatcgacaa atagccaatc atattccggc gaaatcatta
    42301 agttattcat tttttaaaaa tagaaatgaa actgtgtcag atgaggaata tcaatctatc
    42361 gtaaagaatg cccatttaat tgttgatagt gtgcaactag aacgtgattt aattagtcat
    42421 caagagaagt atcagcgaga gaatacaatg attcgaatca cgccatttga aacgagacaa
    42481 tcacctaata taagtagtca attgatggaa acatttatag gtgtatggat agacggtatg
    42541 agtgacgttg atttgcaaca aatgatgcaa cgacttgtgg attatatcgc acaagaagat
    42601 aattaccgtt taattttatt aacgcgccat caaaatgata taccgatgtg gcttcgtgaa
    42661 tgtattacgt cggtaaatga ggaataccaa gctaaacaga atgccgatgt taatgtttca
    42721 gcattaatga cacctgaaga tcaagatgac atcattgctg ttaagacgat acatgctgaa
    42781 catgatgttg tagaagcatt gcggacgttg cgacttgtga tagatatgtc aaaagaacct
    42841 gatttgtatt tacaaattag tgcaattagc gctgggattc cacaaattaa tggtcaacaa
    42901 acagattacg tctctgatta tgacaatggc cgtattataa atacagttga tgaattagat
    42961 gatgcgttaa attattattt attttatttg aaaaattgga attatgcgta cgcctattct
    43021 ttaaaattaa tagatgcata tgcttctaag aatattatta atcagctcga tgagttaata
    43081 gaaggtgaaa atgatgccac gtaaatttag agttttgcaa attggaggag acgatttaga
    43141 acctattttc caacacaaaa aaggtgtgag ttgggattac ttcgatattg gattgtttga
    43201 atttgatagt ggttatgtag aggctattga agcgattgtt gaagcagaag gacgctttga
    43261 ttttatctat attcaagcac catactcgga gacattaacg aatttattac aaatgataag
    43321 cgaaccatac aatacgtatg ttgatgaatc attttggtct gttgaatatg aacaagacga
    43381 aaatgtccaa aaatacgttg ttcaaccatt acattaccgg aacattgaag aacgtaataa
    43441 taaattagag gcagttagct tctcaggaca gtatggagat aaggtttctc ctaagttagc
    43501 acttgttcat ccgaatttta aaggagatgt cgtttaccaa ggtaattcag agctcacgtt
    43561 gagtggagaa tttggaaaag aatttaaacc tatcgcatct tggcaaaata atctcgttta
    43621 cgataaagat aaagtcattc aaatatggcc agaatttgat attgatggtg cggtcgagtt
    43681 gcaatattca tttagattga ttcaaactgg cgctgatggt gcattaattg aacaaatcat
    43741 attgactgat gatatgttag acagtccttt agagatacct acgaaaccat ttgatgctta
    43801 tataagtgta actgttaagg cgcgagggaa cgggacggta catttaggac ctatacacaa
    43861 acgatggtct agattagaca tgggtcaatt tttactcggt ggtagtcgtt ttgtagatag
    43921 tcaacgacag gaatttattt attatttcca ccctggtgat atgaaaccac cactaaacgt
    43981 atattttagt ggttatcgaa cagcggaagg tttcgaagga tattatatga tgaaacgtat
    44041 gaatgcaccc tttttactaa tcggcgatcc tcgtgttgaa ggtggtagct tttatatcgg
    44101 ttcatctgaa tatgaacaag gtattatcaa tgttattgaa gagacattag aaaagctgaa
    44161 ttttaaatca catgaattaa tattatctgg tttatctatg gggtcatttg gtgccttgta
    44221 ttatggtgct cagttaaatc ctcaggcaat tattgttggt aaaccacttg ttaatattgg
    44281 aacgattgct gaacatatgc gattacttag accagaagag ttcggtacag cattagacgt
    44341 cctggtgtct aatgaaggtg atacatctca agcgtcgata caggcattaa atcaaaaatt
    44401 ttggcaaaca tttcaaaaga aatcattgtc acaaactgtt tttgccatag catatatgca
    44461 acatgatgat tatgatccac atgcatttca agagctacta cctgtattga cagcacacca
    44521 agcaagagtg atgaattgca gtattcctgg gcgacataat gatgattcac ctaccattgc
    44581 aagttggttt gttaattttt acaacattat tttggaagac aagtttggga gagttcaaca
    44641 tgctgaaaaa caaaacattt aaagttactt ggaattatat ttcacaaact acatttatgt
    44701 atggctcaaa agtatcattt tcaaatggtg aagtaacttt tataaatcct ttgatgccct
    44761 ctggattacc gattcatgag tggttgatgt taaaacaatt ttcaaaatat aaaagtgcac
    44821 caagtttacc aattttgcgt agaggacagc attataaatt gcattttgat tttgatgcaa
    44881 cgcctgctgg ttcagtgtat tttatcatca ttttttataa taaaaatggc acaaaactaa
    44941 gtactgagat tgtaaagtca aatagtatta cgattcaata tcctgatgaa gcctatgctt
    45001 ataaaataaa aatgatgaat gcagcatcga cgagtcttat atttagatgt ttaacaatta
    45061 cagagatgac gcatcaagat aatttagaat ataaaagcat gcgggtgacg aagataggcg
    45121 atgaccaata tggaaatgat aggatcaatg tcattattgc agaaccatct gacgcctatc
    45181 caactatttc gaatgatttt ttgaaatcgt ttggtcatgt ttggctcgtt gaacgttgga
    45241 tggacgacaa tatcgaggga aatattaagc aactaaagaa tgatttgcaa agtcaagata
    45301 ccctaaaggc aattaatttg attagttatg ggtcaaagtc aaatgttttt gcaacttatg
    45361 tcgctcagca tttggattgc aaagtgtatc gtacaagcca tgaagatgat gatttaaaag
    45421 aatggtttac agaacatgtc ccaggaaatc atgaactaaa agatacaaat gtcgaggttt
    45481 attttaaaga agagcaagac aaccatctaa attatatgtc acgtttaatg aatccggtac
    45541 gatttttaga ttacttagat gtatcgaaac tgaatggtgg ggaagtaaat gaaacataaa
    45601 ctagatgtta cgataaacga attaagacta aagtcgatta gaaaaatagt gaaacgcatc
    45661 aatacatgga gcgatgaagt taaatcatat tctgatgatg tgttaaaaca aaagacacta
    45721 gaatttaaag aacgtatagc atcaggagtt gatacactag acacattgtt acctgaagct
    45781 tatgcagtgg cacgtgaagc gagctggaga gtattaggta tgtatcctaa agaagttcag
    45841 ttaatcggag cgattgtatt gcatgaaggt aacattgcgg agatgcaaac aggcgaaggt
    45901 aaaacattaa cagcaacgat gcctttatat ttaaatgcac tttcgggaaa aggaacatat
    45961 ttaattacaa cgaatgatta tttagcgaag cgtgattttg aagagatgca accattatat
    46021 gaatggttag gtttaactgc ttcattaggt tttgttgata ttgtggatta tgagtatcaa
    46081 aaaggggaaa aacgtaatat atatgaacat gatattatat atacaactaa tggacgtctc
    46141 ggttttgatt atttaattga taatttggca gatagtgcgg aaggaaaatt tttaccacaa
    46201 ttaaattacg gtattattga tgaagtggat tcaatcattt tagatgctgc gcaaacacca
    46261 ttagttattt cgggcgcacc aagattacaa tcaaatctat ttcatattgt gaaagagttt
    46321 gtagatacat tgattgaaga cgtgcatttt aaaatgaaga agaccaaaaa agaaatatgg
    46381 ctgttaaatc aaggtattga agcggcacaa tcatacttta atgttgaaga tttatatagc
    46441 gaacaagcga tggtcttagt gcgtaatatt aatctagcac tgcgcgcgca atatttgttt
    46501 gaatctaatg tcgattattt cgtatataat ggtgatattg ttttaattga ccgtattaca
    46561 ggtcgtatgt taccgggaac taagttgcaa gctggactcc accaagctat tgaagcgaaa
    46621 gaaggtatgg aggtttcaac agataaaagt gttatggcaa caattacctt tcagaattta
    46681 tttaaacttt ttgaatcatt ttcaggtatg acagctacag gaaaattagg cgaatcagag
    46741 ttctttgatt tgtattcaaa aatagtcgta caagtaccaa ctgataaagc gattcaacgt
    46801 atcgatgaac cagataaagt gtttcgttca gttgatgaga aaaacatcgc aatgattcat
    46861 gatatagttg aacttcatga aacggggcga ccggttttac tcataacgag aactgctgaa
    46921 gcggctgaat acttttcaaa agtatttttc caaatggata ttcctaataa tttactcatt
    46981 gcgcaaaatg ttgcaaaaga agcgcagatg atagctgaag cagggcaaat tggttccaag
    47041 actgttgcga ctagtatggc aggtcgaggc acagatatta aacttggtga aggtgtcgaa
    47101 gcattaggtg gattagctgt tattattcat gaacatatgg aaaatagccg tgtagacagg
    47161 caattacgtg gtcgttctgg tagacaaggg gatccaggat catcttgtat atatatttca
    47221 ctagatgatt atttagttaa gcgatggagc gatagtaatt tagcggaaaa taatcaatta
    47281 tattcattag atgcacaacg attatcgcaa agtagtttgt ttaatcgcaa agttaagcaa
    47341 attgtagtta aagcgcagcg tatctcggaa gaacaagggg ttaaagctcg tgaaatggct
    47401 aatgaatttg aaaaaagcat tagtatacag cgagatcttg tatacgagga acgcaatcga
    47461 gttttagaaa tagatgatgc tgagaatcga gattttaaag tgttagctaa agatgtgttt
    47521 gaaatgtttg taaatgagga aaaggtgcta acaaaatcgc gtgttgtcga gtatatttat
    47581 caaaatttaa gtttccaatt taataaagat gtggcttgtg ttaattttaa agataagcaa
    47641 gctgtagtaa catttttatt agagcaattt gaaaagcaaa tagctttgaa tcgtaaaaac
    47701 atgcaaagtg catattatta taatattttc gttcaaaaag tctttttaaa agcaattgat
    47761 tcatgttggt tagaacaagt cgactattta caacaattaa aagcaaacgt taatcaacga
    47821 caaaatgggc aacgtaacgc tatttttgaa taccatagag ttgcattaga ttcatttgaa
    47881 gttatgacac gaaatatcaa aaaaagaatg gtgaaaaata tttgtcaaag catgattaca
    47941 tttgataaag aaggtatgcc agttatacat tttccataaa cgaaaagggg tagcgcatga
    48001 caatatataa tataaacttt ggtataggct gggcgagtag cggtgttgaa tatgcacaag
    48061 cttaccgtgc taaattattg agacgcacaa atcaagacac taaatttgtg tttttagatt
    48121 ttatacagtc agagaacata cagacattaa cgcaaaacat tgggtttgaa gatcaagaag
    48181 ttatttggtt gtatcagtat tttagtgata tttctatagc accaacaacg tacacgttag
    48241 atgacattga acgtgagctt ggtcatgata ttagttttag agaaagaaat ggtaagatag
    48301 tccgcctata ttttaatggg aaatcatcat ttgtaacatg ttatctgcaa aatgaacaaa
    48361 aagacattgt ggatcgtgca gaatttgtaa ttaatagtat gttagtacgt aaagattttt
    48421 atagttatac acgcatattt tctgagtatt atgcaccagc tgataataaa gcgaaattat
    48481 atatgcgaca attctataat gaaaatggct ccatcgctta taacgaatat attgatggag
    48541 acaactctat atatgtgttt gcagatgcgg tgttgtataa caaaacggaa tttattgcat
    48601 actttttaca acgattgaat ttaacccgag acgatattgt catattagac cgcgcttctg
    48661 atattggtca ggctgttttg cagcataaag gtgatagtaa agttggtgtt gtgattcatg
    48721 ctgatcatta tagtaataat atgatgagcg agcagcatat actatggaac aattattatg
    48781 aatatcaatt ttcaaaagct aaatacattg atttctttat tacagcgaca gatattcaaa
    48841 atcatatggt ttgtcgacaa tttgagcaat atcaaggtta tcgcccacgt gtttatacta
    48901 ttccggtggg aagcattgat gcattatcat atccaacatt atcaagaaag ccatacgcga
    48961 tgattagtgc atctcgttta gcaaatgaga aacatattga ttggcttgtc aaagcagtta
    49021 tcgtggctaa acgacaagtg ccagaattaa catttgatat ttatggagaa ggtagtgaga
    49081 agacgcgact gcgtaaaatt attgatacac accgcgcgca agattatatt cgtttactag
    49141 gtcatgttaa acttgatgaa atttatactg attatgagtt atttctttct gcatcaacta
    49201 gcgaaggttt tggattaaca ctaatggaag cggtaggctc aggactaggt atgattggat
    49261 taaatgtgaa ttatggtaac ccaaccttta ttcgagatgg ggaaaatggc tacttggtac
    49321 cttttgatac cgatgaagat agtgtggatg atgttgtcgc aaaactagct catgccattg
    49381 taatgtattt taacaatgga ccccagacac cacattacat ctcttatgaa gtggcacagc
    49441 aatttatgac tcaagatatt attttaaaat gggaaacatt agttcaagag gtgctgcatg
    49501 attaaattat ttgattactt taacggtcat tcacgaaagt tatatgagtc ttttaaagcc
    49561 agtaagttgg aggaagattt aacaattgta ctcaatgaca atggtttttt accggacgat
    49621 gttatatcac cgtatcaatt ttttgcagat aatcataata cagaaaatat gaaaccacga
    49681 ttttttaatc aggttactgt accagctttt tgggaaatta aaggtaacaa caattcggct
    49741 acaattaatg atatgggacg tctacgaggc aaaatatttt atcaaagtgg agagcgccct
    49801 agaattgtta gtcgtgtcga atggtttgat gaccaacaac gtgtccgatt tgtcgattat
    49861 tattctaaga atggtattaa atttgcacaa actgtttacg atttaaatcg taaagcgatc
    49921 ttgaagaaat atatgacagc agaaggtaaa gaagtgattt atgaaaattt tgtaacatct
    49981 gatgtcatat tggattggca aggaaagtct tactttttcc cttcaaagct cgcattcgta
    50041 ttatttttta tcaagcaact tgaaattacg gaacatcatt tcgttattaa ctcactagcc
    50101 ctaccatttt cagtgttata taatttgcca tcaaatggta gtgatgtttt ggtatggcaa
    50161 gaacagtgcg atggtaatgt cccgggaaat atgcaattga tgtgtaaagg tgatatgaaa
    50221 cgacattgta atattattat tccagataaa aacgaatatg aaacgatgct caatatagca
    50281 gacgcaaagg ttaaatctcg tattttacaa ggcggatatc tatataatta tcgtagtcgt
    50341 aatagatata ctaaagaaat tgtaatatta actaactcgg atcaacttcg taacatcaag
    50401 gtattggttg aaactttacc agattttaag tttcacatcg ctgctattac agagatgtct
    50461 gataagctaa tgcaacttga tcaatatgct aatgtgcact tatacccatc tattaatata
    50521 gacagggtaa atgaactata ccagttatgt gatatttatc ttgatattaa tgagggtaat
    50581 gaaattttaa atgcggtgga acaagcgttt gactatgagt tgttgatttt aggttatcgt
    50641 caaacagcac accatgctaa agtaacacta tcagagcact tatttgagca taatgatgag
    50701 ataacaatgg aaagtaaaga ccaattaata caaatgttag aaagtttgaa agatcaacaa
    50761 cagtttcgag atgctttatt agcacaaaaa gcacatgcac acgaaatctc gcgtgaacaa
    50821 ttcgagcaag tttttaaaca ggcgttcgaa agctaaaaag aaaaagcagt ctaaaataaa
    50881 acatcattta atataaaaga tttgacgtaa tatgatgtta gcgacatgga caatatgata
    50941 ttacgtcatg cttgttttgt ttacttaaat ataagctaag taataagtag ataattacta
    51001 acaacaataa ctagatagat aagtgtaaat ttcttgtaaa caggtatata atagtatgta
    51061 attcttttaa ggaagtaagt gagaaaggag tatgttgatg gctaaatatc gagggaaacc
    51121 gtttcaatta tatgtaaagt tatcgtgttc gacaatgatg gcgactagta tcattttaac
    51181 gaatatcttg ccgtacgatg cccaagctgc atctgaaaat gatactgaaa tttcaaaaga
    51241 gatattatct aagcaagatt tattagacaa agttgacaaa gcaaatcgtc aaattgagca
    51301 attaaaacag ttatcggctt catctaaagc acattataaa gcacaactaa atgaagcgaa
    51361 aacagcatcg caaatagatg aaatcattaa acgagctaat gagttggata gcaaagacaa
    51421 taaaggctct caaattgaaa tgaacggtca aagtgatata gacagtaaat tagatcaatt
    51481 gcttaaagat ttaaatgagg tttcttcaaa ggttgatagg ggtcaacaaa gtggcgagga
    51541 cgatcttaat gcaatgaaaa atgatatgtc acaaacggct acaacaaaac atggagaaaa
    51601 agatgataaa aatgatgaag caatggtaga taaggcgtta gaagacttag accatttgaa
    51661 tcagcaaata cgcaaatcga aagatgcatc gaaagatcca gtagtgtcta caacagataa
    51721 taatcatgaa gtagctaaaa cgccaaataa tgatggttct ggacatgttg tgttaaataa
    51781 atttctttca aatgaagaga atcaaagcca tagtaatcga ctcactgata aattacaagg
    51841 aagcgataaa attaatcatg ctatgattga aaaattggct aaaagtaatg cctcaacgca
    51901 acattacaca tatcataaac tgaatacgtt acaatcttta gatcaacgta ttgcaaatac
    51961 gcaacttcct aaaaatcaaa aatcagactt aatgagcgaa gtaaataaga cgaaagagcg
    52021 tataaaaagt caacgaaata ttattttgga agaacttgca cgtactgatg ataaaaagta
    52081 tgctacacaa cgcattttag aaagtatatt taacaaagac gaggcagata aaattctaaa
    52141 agatatacgt gttgatggta aaacagatca acaaattgca gatcaaatta ctcgtcatat
    52201 tgatcaacta tctctgacaa cgagtgatga tttattaacg tcattgattg atcaatcaca
    52261 agataagtcg ttgttgattt ctcaaatttt acaaacgaaa ttaggaaaag ctgaagcaga
    52321 taaattggct aaagattgga cgaataaagg attatcaaat cgccaaatcg ttgaccaatt
    52381 gaagaaacat tttgcatcaa ctggcgacac gtcttcagat gatatattaa aagcaatttt
    52441 gaataatgcc aaagataaaa aacaagcaat tgaaacgatt ttagcaacac gtatagaaag
    52501 acaaaaggca aaattactgg cagatttaat tactaaaata gaaacagatc aaaataaaat
    52561 ttttaattta gttaaatcgg cattgaatga taaagcggat gatttattga atttacaaaa
    52621 gagactcaat caaacgaaaa aagatataga ctatgtttta tcaccaatag taaatcgtcc
    52681 aagtttacta gatcgattga ataaaaatgg gaaaacgaca gatttaaata agttagcaaa
    52741 tttaatgaat caaggatcaa atttattaga cagtattcca gatataccta caccaaagcc
    52801 agaaaagacg ttaacacttg gtaaaggtaa tggattgtta agcggattat taaatgctga
    52861 tggtaatgta tctttgccta aagcggggga agcgataaaa gaacattggt tgccgatatc
    52921 tgtaattgtt ggtgcaatgg gtgtactaat gatttggtta tcacgacgca ataagttgaa
    52981 aaataaagca taattatatt gggggaagtg catctatata ttttttaagt atataagacg
    53041 tcttatttcc cccttaaatt attgtgaagt atatgcaaaa tgcaatgaat agattgtcca
    53101 tcattttaac gttataatga atttaacgac ttagaactac acaagtaaag gagaataaag
    53161 atgtctcgaa aaacggcgct attagttttg gatatgcaag aaggtatagc gagtagtgta
    53221 cctagaataa agaatattat taaagcgaat cagagagcaa ttgaagcagc aagacaacat
    53281 cgaataccag tcattttcat acgtttagtg ttagataagc attttaatga tgtctcctcg
    53341 agtaataaag tgttttcaac aattaaagct caaggatatg cgattactga agcagatgca
    53401 tctacacgaa tacttgaaga cttagcacca ctagaagatg agccgattat ttctaagcga
    53461 cgctttagcg catttacagg tagttacttg gaagtttatt tacgtgcaaa tgatattaat
    53521 catttagtat taacgggtgt ctctacaagt ggagctgtat tgagcacggc attagaaagt
    53581 gtagataaag actattatat tactgtttta gaagatgctg ttggtgatag atcagatgat
    53641 aaacatgact ttattattga acaaatttta tcacgctcat gtgacattga atccgtagag
    53701 tcatggaaaa gtagtttata gtaaatataa cgtcaattaa agctcggcag taatgtttga
    53761 gaataagtac atttgctcat atttataaaa tgtgtgagat gacaattgaa acggatatgt
    53821 tgagaaacat tagaacataa aataatatat ttatataaaa tgacccgagg cgttcgaact
    53881 gaatgcctcg ggtttaattg aataagaaat cggacttatg aacagaaata tgtttaagtc
    53941 cgaactcctt gtttatactt ataaatttta cgggtttaat ataatactta tttacctgta
    54001 atatatgata attcttcagc ggcagatgcg ttgatagttc tatgagaaat gatacctaat
    54061 cctttaacat tggattctga aataacgata gaaccatcac tgttaacttt ttcaacaaat
    54121 gctacatgac cgtaatgttg atctgcacca aattgtccag cctcaaatac aacagcagca
    54181 tgacgttttg gtgtatgact tacttgataa tcacggtatt gagctcgatt attccaatta
    54241 tgtgcatcac ctaaatcacc tgagatagat gtaccaaatt gtttcatacg gttatatacg
    54301 taccaagtac attggccatg tggatatggc atactatcag atacctcacg gaaaggtttg
    54361 aattcatctg atgaatcatc ataatccttg atagaacgtt catatttatc taaatctggc
    54421 atgcgttcat cgtcaaactg agttaattga tagtgtttaa taatactgtt taatttctta
    54481 gcatagtttg gatctgtagc atatgtttta gataagtgtg atgttgcatc tttataagaa
    54541 tcggcttccg atttccatgt tggtttataa attgttcgat tgccatcaat accattttta
    54601 ataaggtcag agtaatcttt tagtgattct ttcgtgcttg gatattttcg gaatccagca
    54661 ttaatactat acaattgatt accatcagct tctaatgtgt taaaaggaac agaattccct
    54721 ttaaaagcac ctttgatacc gaataaattg tggtttggtg acttagctaa agcactacga
    54781 cctgagtcag attctaagat tgcttgggca atcatgacag acgcataaat atcgttatct
    54841 tgaccaatgc gatgtgcatc tttagcaatt gatttgacaa attgacgtgt atcttttgag
    54901 tcaacaacgt taaattgtcc actatcatca ttgttagata tactaggatc tgtttcgaat
    54961 aatgatgttg cacgtgtatc cttttgatta acatcgttat tgaatgattg agcaggttta
    55021 gatttatgtt tcaattcatc ttgtgttggt aactgtggat tctttgtatt agatttttca
    55081 tttttgtctt ttttagattg agatgcgtaa tctttttgtg ttttctttgc atcttcactg
    55141 tattgatcca aaatagagtc taaagccgaa tctgacattg attgattatc tttcgatgaa
    55201 gatttttgat ttgctttatc gtcacttgct ggttgactat ttgattgatt tggttgtgtt
    55261 ggctttggcg aatttgattg cttattagat gtacttggtt ttgtattgtt tgatttaggt
    55321 gctttttgat tgtctgcttt atcttgttta gatgattgcg tatccgtatc atttttgatg
    55381 ctattatcac tgtttttatt cgaatcattt gttgactttt caccattacg aggttgttcg
    55441 taatcagaaa tatccgaatt taaattgaat aagttttgga ttaaagttgt taatgagtaa
    55501 ttatcatcgt atttattttt ggttagcaat tggtttatat tggtttgtgg taaattctta
    55561 taaataaaat caatgatatt gttagagtct gaagtgctgt cgtctatagt tttaactttt
    55621 ttgtcgttat tgtcttggtt actagtatta ttgttgtctg ctttatcagt atctttactt
    55681 gtagtatcct tagaagtttc atcatcagta gattttttgg aatcatgaga tgttgtctta
    55741 gctgtagtat ctttttgagg tgtgtcagca taagcggtag gtgaaactaa agtaggtaat
    55801 acgagcgtag ttgatagcaa ataaattaaa attttatttt taggcatatt tcgtattctc
    55861 ccttgaaaaa tataataatt aagtgtgata ataaactatg atttgttata atttatcgta
    55921 tgctgaaaat agttgatagg tatcaatcga ctaaatatct tccagtaaat tgattatact
    55981 aattcacaac gcaaaaataa attaatttac aaaaaatata taaaaaatat gaataattcc
    56041 tacataggag tgtgacaatg aagaacgcat ttaaattatt taaaatggat ctgaagaaag
    56101 tagctaagac gccagctgtg tggattatct tagcaggctt agctattttg ccatcgttct
    56161 acgcttggtt taacttatgg gcaatgtggg atccatatgg caacacggga cacatcaagg
    56221 tcgcagtcgt taatgaagat aaaggcgaca caatcagagg gaaaaaagtt aatgtcggta
    56281 atacgatggt taatacactc aagaaaaata aaagttttga ttggcagttt gtaagtagag
    56341 agaaagctga tcatgagata aaaatgggta aatattttgc aggtatttac atcccatcta
    56401 agtttacaca tgaaattacc gggacactac gtaagcagcc tcaaaaagca gatgtagaat
    56461 ttaaggtgaa tcagaagatt aacgctgttg cgtctaagct aacagatact ggttcgtcag
    56521 ttgtcgtcga aaaagcgaat gaacaattta ataaaacagt aactcgagca ttattagaag
    56581 aagctaacaa agcaggttta actattgaag aaaatgtgcc gacaattaat aagataaaaa
    56641 atgcggtata ttcagcagat aaagctttac ctaagattaa tgactttgcg aataaaattg
    56701 tatatttgaa taaccaccaa gcggatttag ataaatatgc gaatgatttt agaaaactag
    56761 gaaattataa aggtgatatt ttagatgctc agaaaaaatt aaacgaagtc aatggtgcta
    56821 ttccgcaact taatgaaaag gctaagttga tcttagcttt aaataattat atgccgaaaa
    56881 ttgaaaaagc gttaaatttt gcagctgatg acgtgccagc gcagttccct aaaattaatc
    56941 aaggacttaa cattgcgagt caaggtattg atcaagctaa tggacagtta aatgatgcca
    57001 aaggcttcgt cacacaagtt agaagtagag tcggtgatta tcaagatgca attcgacgcg
    57061 cgcaagattt aaatcgaaga aaccagcaac agattcctca aaatagcgcg gcgaacaacg
    57121 aaacatcaaa tagtgcacct gtagctggta atggtgtagc atcaacgcca ccaagtgcac
    57181 caaatggcaa tactgcacca aataataatg ttacgcaaaa taccgcacca aatagtaata
    57241 atgcgcctgt atcgactaca ccacaaagta caagcgggaa aaaagatgga caaagttttg
    57301 cggatataac aacaacacaa gtcagcacag caaacgagaa cacacaaaac attacagata
    57361 aagatgttaa atcaatggaa gcggcattaa cgggctcttt attatcatta tcaaataatt
    57421 tagataccca agcgaaagcc gcacaaaaag atagtcaggc attacgtaat atttcgtatg
    57481 gcattttagc atcggacaag ccttctgatt ttagagagtc tttagataat gttaagtccg
    57541 gtttagaata cacaactcaa tacaatcaac aatttatcga tacattaaaa gagattgaga
    57601 agaatgaaaa tgttgattta tcaaaagaaa ttgataaggt aaaggcagct aataatcgaa
    57661 ttaatgaatc attaaggtta gttaatcaat taagcaatgc attaaagaat ggtagttcag
    57721 gaactgctga agctactaaa ttactagatc aactgtcaaa actagattca tcattatcat
    57781 catttagaga ttatgttaaa aaagatctta acagctcttt agtatcaata tcacaacgta
    57841 ttatggatga attgaataaa gggcaaacgg cattgtctaa tgttcagtct aagctaaata
    57901 caattgatca agtcatcaac agtggtcaat ctattttaaa aaatggtaaa acacgtatcg
    57961 atcgtttaca aacagtatta ccaagtattg aacaacaata cattagtgct gttaaaaatg
    58021 ctcaagcaaa cttccctaaa gtgaaaagtg atgtagcgaa agcagctaac tttgtacgta
    58081 acgatttacc acagttggag caacgtttaa ccaatgcgac tgcaagtgta aataaaaatt
    58141 taccaacgtt attaaatggt tatgatcaag cggtaggatt actaaataaa aatcagccac
    58201 aagcgaaaaa ggctttatca gatttagctg atttttctca aaataaattg ccagatgttg
    58261 aaaaagatct gaaaaaagcg aataagattt tcaagaagtt agacaaagac gatgcagtcg
    58321 ataaattaat cgacacactt aagaatgatt tgaaaaagca agcgggtatt attgcaaatc
    58381 ctattaataa gaagactgtt gatgttttcc cagttaagga ttatggatca gggatgacac
    58441 cattctatac tgcattatcg gtatgggtag gcgcactctt aatggtaagc ctattaactg
    58501 ttgataataa acataagagc ttagagccag tgttaacgac aagacaagtt ttcttaggta
    58561 aggcaggatt ctttataatg cttggtatgt tgcaagcact cattgtatcg gttggagatt
    58621 tgttaatcct aaaagcagga gttgagtcac ctgtattatt cgtacttata acgattttct
    58681 gttcgattat tttcaactca atcgtatata cgtgcgtatc attacttggt aacccaggta
    58741 aagccattgc aatcgtattg cttgtattac aaattgcagg tggtggagga acattcccaa
    58801 ttcaaactac gccacaattt ttccaaaaca tttcaccata cttaccattt acgtatgcca
    58861 ttgattcatt acgtgaaaca gtaggcggta ttgttccgga aatcttaatt acaaaattaa
    58921 ttatattaac gttatttggc ataggattct tcgttgtagg tttaatttta aaacctgtaa
    58981 cagatccatt gatgaagcgc gtatctgaaa aagttgacca aagtaacgtt acagaataaa
    59041 aatgaaatcc acacactagg gttctatcac cttaatgtgt ggatttttat gtttttagac
    59101 agaagagata gtaatttctg tcttttatgg gaagggtgtt atcattgcta ttatccagga
    59161 tgacttacta tagcactaat attaccgaca aagtgaatat cctcgtcttc cgtagttaaa
    59221 ataaagctag aacctttttg gatgtcatag tgcttatcgt ttactgctaa agtaccagta
    59281 ccatcgataa ttgtaactaa gcaataagca tgtggtttat tgaattttaa atctccatga
    59341 atatcccatt tatatactgc aaaatattga ttgtctacaa attgagttac agtgtgtgtg
    59401 tcgatgtgag ttgttatagg agtagtattt ggttcatgat tgcctaattc aatcacatct
    59461 ttactttgct ctaagtgcaa atcacgcaat tgaccatttt gatctcgtct atcatagtca
    59521 taaatacggt atgtcgtatc ggacgattgt tgtgtctcta aaattaaaat acccgaacca
    59581 atggcatgga cagtgccagc aggaacataa taaaagtcac cgggcttaac aggtatacgt
    59641 ttgaaaagac tgtcaaattc atgattatca atcatgtcga ttaacgtctg tttattatgt
    59701 gcatgtacgc catatataat ttcagcacct ggggctgcat ctaaaatata ccaacattct
    59761 gttttaccta gttcgccttc gtgttttaaa gcgtaataat catccggatg aacttgaaca
    59821 gataatttat cattggcatc taatacttta gttagcagag ggaaactatc tcgtgaatca
    59881 ttatcgaata attcacgatg ttgtgaccaa agttgatcta gggtcatatc cttgtatgga
    59941 ccattgataa ttgtattagg gccatttgga tgtgcagaaa ttgcccagca ttcaccagtt
    60001 gtttcattag ggatatcata gttaaatgct tttaatgcat gaccgcccca aattctgtct
    60061 ttaaaaacgg gttgtaaaaa taatgccata gttaaaactc ctctatattt tcattaataa
    60121 gttataaatt tctgtagtac tgtttgcatt aattagtgat tggcgcgtct catcattcat
    60181 taacgcttta gataagcgct gaagtatttt taaatgtgta tcctgactgt tgtttggtac
    60241 ggcaattaag aaaatcaatt gaggtagact accatctaga ctttcccatt taacaccatg
    60301 attatttttc ataacagcta caatcggttg ttttacaaca tcagactttg catgtggaat
    60361 ggccacgttc atgccaatag ctgtcgtaga ctccatttca cgttctagga ttgcattttt
    60421 taaatgcgat gtgtgctcta cataacggca cactttaagt tgatgaatca acgtatcgat
    60481 tgcttcgttt cgagacatgt tctgatcagc aataatgata gtttgttgat taaacacatg
    60541 agaaggttta ttgagatgtg aatgtttcgc ggtgttatct acattgtcaa cctctgtatc
    60601 atgttgtatt atatctgtag catgaagttg cgtgtgttgc gctggtgcat ctactgctat
    60661 aactggtgtg cgacgtttta ataataagac agtgatcatt gtgacaagac tacctattat
    60721 cactgcaaag ataaaccata atatatgatc aataccacct aatacggcta caattgggcc
    60781 gccatgtgcc actctatctc caacaccacc gatggctgca aggactgatg caatcatcgc
    60841 tccaatcatg ttagcaggta taatgcgtag tgggtcttga gctgcgaaag gaatagcacc
    60901 ttcagtaata ccaaatagtc ccatagtgaa ggaagcctta cccatttctc tttcggaatg
    60961 attgaattta tacttttgaa caaacgttgc caaaccaaga ccgatgggtg gtgtacatac
    61021 agctacggct accattccca taacagcata atttccttca gcaatcagtg ctgaaccaaa
    61081 taaaaatgct actttgttta ctggaccgcc catatcgaag gcgatcattg ccccaataat
    61141 cagcgcaagt ataataatat tagcaccttg catacctttt aaccaggttg ttaatgcctc
    61201 aaaaatactc gaaatcggtg cgcctattaa aaagatgaaa attaacccca caataacgga
    61261 tgaaataatg ggaataataa tgataggcat aattggtgcc atcgcttttg gaactttaat
    61321 atctttaatc cactttgcga tataacctgc taagaaacca gcaacaatac cacctaaaaa
    61381 tcctgcacct gcatcactgc cataaaaact accgtcagca gcgatagcgc cgccaatcat
    61441 accaggaaca agaccgggct tgtcagcgat actaacagcg atatatccag ctagtattgg
    61501 gaccataaat ttaaaggcta aactaccaat gttttcaatg gatttccaaa atgaatcatc
    61561 cgggatgact aatccttttg atgtcgtttc accgcctaga gtcagcgcga tggcgataag
    61621 gagtccacca actacgataa aaggaaccat aaacgataca ccgttcatta aatgttgata
    61681 caccatttga ataccacttt tagacttacc gcgatctttc gaatgataat ttgtttcaga
    61741 ttgataaata ggcgcatctt gattaatgat actttgaatt agacctctcg gattatgaat
    61801 cccttcgcga acattttcat taatcaaccg tttaccaaca aatctggaca gatcaacttg
    61861 tttatcagct gcaattatga cgccgtcagc ttcttcgatg tcttgcgtag ttaaaacatt
    61921 ttcagcacca acaccgccct gtgtctctac tttaatatcc acacccattt cttttgctac
    61981 ctgctcaagc ttttcttgag ccatatatgt atgtgcaatg ccatttgggc atgaggtaat
    62041 agctacaatt ttcataaaat catctccttt tctatattgt aagcgtattc ttgatactaa
    62101 aaaaaagaat aattaccgtt actagtggca attattcttg taagtattca aataactgtt
    62161 gctttaaact atgatcatct aaactacata aatgattcac tgaatcatca tccaagttag
    62221 caattaattg catcatttgt tttgtaaaag ctttgtcttt atgtgaaatc gctaagaaaa
    62281 agacaagttt gacatcgtgt tgtcgccaag gaaaaacatc ttttgtgcga aaaataagca
    62341 catgtgattg taaaactttt tcaggatctc catgaggaat cgccataaaa ttacctatat
    62401 atgtagaaga tgatttctca cgctctaaag ctgattcgat atatccttct acaatcgcat
    62461 gatgtgcttg taatattttt tgagcttctt caaaaatttg cacagtatgt cgtggttttt
    62521 gttcagtatt tacgacaagg aaattgacag tgtccatatg atgatgtgct tgaaccggat
    62581 tttgcttttg cttcacaacg tgtctgattt tgtggcggtc atcttcagaa aataatggtg
    62641 aaaccttgat agtcgttagg tgcttaggaa gtatgtttag cgtttgtttt ggaatatcat
    62701 gagtcgttat taataaatct acattatcaa agtgatagtg tgttatattt tctagtttaa
    62761 tcgtatttat cactgacaac tcttcggata agttgtttat tttagtttct aaaaaattcg
    62821 acacacctag accataataa caagcaatga ctacatttaa ttgtgttttg gtacgacgct
    62881 cgatggcagc ttgaaaatga attgttaaaa aggcaatttc atcttcgctc atctctatat
    62941 cagtatcaat tgctaattta tcaatcgctt caaaaagtgt gttgaacaca aagggataaa
    63001 gttttttaat ctctataact aaaggattgt ttaaataaat gttttgagtg atacgtaaat
    63061 atgctttact aaaatgatta tataaatttt gttgtaaaat cgaatcttca ttgaaaggta
    63121 catgaatacg ttgctgcatc aattcgatta agcgatcaat ataactttgt ataaatatac
    63181 gttctatgcc aatatcgagt ttgttaaaat gataagcaat aaagaatgaa aacatattga
    63241 ttactttttc gtttaaatca taacctaatc ttgtattgat ttgctgaatg caagattgag
    63301 atatcaattt tgcatcgtgg tttaacgtgt ctagctcact taaagattgc ttacgtttaa
    63361 tgattaaaat gagatgaatt aaaagctgtt gtatttgaat atcagttgtt tcaatactat
    63421 gttgttgaag tgtctcttgt ataatatgcg acatcatccg tcggtgggca tcgggtaatt
    63481 catttaaaat aagatcttca acgttaacgt gtccggttga taattgattt aaatgaataa
    63541 tggcatttgt tatatcatta tcagttccgt cgacaataat gccttgtttt ttcttgattg
    63601 ttaaagcaat attaaatttt tgacaccatt gttgaattcg aacgaaatag tcgttgagca
    63661 cagttttaga taatagataa tcttgctcta gttgttgcaa tgtctgcgat ttattgtgca
    63721 ttaacagttg atacgccata gataataata gtttttcttt atatgattga atatgcgtgt
    63781 aacgttcagt tatcgtttct aaggtatact gcgatgtatt taattgatat cctttagatt
    63841 taatacttac gatgatatca tccataaaat tactattgat aacatggata tcattgcgaa
    63901 ctgtgcggtt tgaaacattg acatgttcag caatttcatt agaactaatg tgctttgatg
    63961 ggtttttaat aaaaaactgg agtagtttta agtgtctatc aagcatttaa catgtacctc
    64021 ctttctaaat ttttcgtgta agcgttttta aaggtgtatt aatattatta aacatgagag
    64081 cttatacata cgtcaatgac attaaagcga acttttatat gaatttaaca gagtgagaat
    64141 tatgcaaata aagaacagca gtaagatatt tcaaatagaa aaatatctca ctgctgtttt
    64201 tctgaattta tgtacctgat acttgtggac gtatcaggta aatgattaat ttttaggtga
    64261 ttgtgcttga ggtgtttgtt tagaaggcgt attggtatta tttgatttat tagcaggtgg
    64321 tgtgttctgt tgttgattat tttggttgtt tgttgacgat tgactgccac cgccgtgatt
    64381 tgaatttgat tgattcggat tagatggttg tgtattcgct ggtgctgttg aaccgttatt
    64441 ttggacagtt gatggcgtag atgattgatt gccattagta acaccgttgt tgttttgatt
    64501 ttcatttgtg ttgtttgtat tttcgtcttt ttgatgattt acttcagtat ctttcttttg
    64561 atgggtagct ttattatctt gctcattatt atgatttgac gatgtagaat gatttattgc
    64621 agcataaatg gcgaatccaa gtgcaccaat taacaagatg ataacaatag taataagaaa
    64681 ttttttcaaa tgtaatgcct ccgtttataa taaaaatagt catacattgc atggcatcat
    64741 gctttcaatg tcgacaaaat gatgttaact ataaaacttg aaatgcggac gatattaatg
    64801 tttgtgtcag tttttatgta cttcaaataa gtataacaaa actgttatat atttataaat
    64861 ataatcaata gtattttatt gtacaagcaa gttataataa tagtaataag attgaaaaat
    64921 ttgtatggaa aagagagata aatatgaatc aaacaactaa actttgtgca gcaacgacat
    64981 tagcattagg tactttaatt ggcgcaacag ttgtagggac tgcatcacca atacatcaag
    65041 aagcacaggc ggcaacaaca ccttactata cttatcatgg atatatcggt cataacgcta
    65101 attttataat ggataagcat tttattaacg ctattaaata tgacaacgtg acatttaatg
    65161 gcattaaatt agcgaagaca aatgcgacaa aaaaagtaga gaaatacgat caaactttta
    65221 aaggtgtaac tgcaaaagga aatgaagcga atcaattaca atttatcgtt aaaaataata
    65281 tatcactaaa agatattcaa aaagcttatg gtaaagattt gaaaaaagag aataataaaa
    65341 cgaaagaatc tgatagcggt attttttatt atcaacattc taaaaagtca ttaggcattt
    65401 ggtttgttgt tgatcataat agagtggttg aagttacagt aggacatacg ccatacaaaa
    65461 cgagtaaata ataaagtaaa tataggggct agtatgtgag gaatgatgtc acatactagt
    65521 cttttttgat tatatgatgg tagttttcgt gtctccatga tattgaagta tatagattta
    65581 atcatgcgca gttaagtagt cagcatatat atttcacgtg ttgattaagt atgtgttgta
    65641 ctaatttttt gattgttaat aatttcacaa aaataaagcc ttgataaatt ttaaaagata
    65701 attaagtgca attttcaaaa ttttatttag tcacagataa catttttaaa aaagaaaaga
    65761 atcaataaat aaaatcaacg aacaaaaagt atagaaataa atagaaataa tcgtttactt
    65821 ttctgaaaaa ttaaattaat attttattta taagctgttt ttaaaatttc aggaggaatg
    65881 aaatgtgagg aaattttcaa gatatgcatt tacaagtatt gcaacagtaa cgttgctgag
    65941 ctctttgaca cctgcagcac tagcgagtga tacgaatcac aaaccagcaa cttcagatat
    66001 taattttgaa atcacacaaa agagtgatgc agttaaagca ttaaaagagt tacctaaatc
    66061 tgaaaatgta aaaaatcatt atcaagatta ctctgttaca gatgtaaaaa cagataagaa
    66121 aggattcacg cattacacgt tacaaccgag tgtggatggt gtgcatgcgc ctgacaaaga
    66181 agtgaaagtg catgcggaca aatcgggtaa agtcgtttta atcaacggtg atactgacgc
    66241 aaagaaagta aagccgacaa ataaagtgac attaagcaag gatgaagcgg ctgacaaagc
    66301 atttaacgca gttaagattg ataaaaataa agctaaaaac ctccaagatg acgttatcaa
    66361 agaaaataaa gtcgaaatcg atggtgacag taataaatac atttacaata ttgaattaat
    66421 tacagtaaca ccagaaattt cacattggaa agttaaaatt gatgccgaca caggagcagt
    66481 tgttgaaaaa acgaacttag ttaaagaagc agcagcaact ggcacaggta aaggtgtgct
    66541 tggagataca aaagatatca atatcaatag tattgatggt ggatttagtt tagaggattt
    66601 gacgcatcaa ggtaaattat cagcatacac ttttaacgat caaacaggtc aagcgacatt
    66661 aattactaat gaagatgaaa acttcgtcaa agatgatcaa cgtgctggtg tagatgcgaa
    66721 ttattatgct aaacaaacat atgattacta caaaaataca tttggtcgtg agtcttacga
    66781 taaccatggt agtccaatag tctcattaac acatgtaaat cattatggtg gacaagataa
    66841 cagaaataac gctgcatgga ttggagacaa aatgatttat ggtgatggcg atggccgcac
    66901 gtttacaaat ttatcaggtg caaatgacgt agtagcacat gagttaacac atggcgtgac
    66961 acaagaaacg gcgaatttag agtataaaga tcaatctggt gcgttaaatg aaagcttttc
    67021 agatgttttt ggatactttg tagatgatga ggatttcttg atgggtgaag atgtttacac
    67081 accaggaaaa gagggagatg ctttacgaag catgtcaaac ccagaacaat ttggtcaacc
    67141 atctcatatg aaagactatg tatacactga aaaagataac ggtggtgtgc atacgaattc
    67201 tggcattcca aataaagcag cttataacgt aattcaagca atagggaaat ctaaatcaga
    67261 acaaatttac taccgagcat taacggaata cttaacaagt aactcaaact tcaaagattg
    67321 taaagatgca ttataccaag cggctaaaga tttatatgac gagcaaacag ctgaacaggt
    67381 gtatgaagca tggaatgaag taggcgtgga gtaaaaatat ataaacaaga agaagtaatg
    67441 ttaaacactt ataaataatt aaattttaaa atacagctta tatatggggt atacctacat
    67501 tgataattga ctttgtaggt gtaccctctt tttgtaatta gatgagagga gtgtgagggt
    67561 tgtctgccga aagactactc ggcagtctaa aattattact agtagtaaat atgtgataat
    67621 taaatgctga cttagaatac aaaaatcatt ttaaaagttg tcacaaaaaa tttacatgta
    67681 tttttattat cttttgcaaa acaaagtgtt aaattataaa tgaaacatgc atgaatttat
    67741 tttttaatac aagaaacgta actaccaaag gagtttacaa tatgaagaaa agtaaacgat
    67801 tagaaattgt ttctacaata gttaaaaagc ataagattta taaaaaagaa caaatcattt
    67861 catatattga agaatatttt ggtgtaagat atagtgcaac aacaattgct aaagacttga
    67921 aagaacttaa tatatatcgt gtccctatcg attgtgagac atggatttat aaagctatta
    67981 ataatcaaac agaacaagag atgagagaaa agtttaaaca ttattgtgaa catgaagtac
    68041 ttagctcgat tatcaatggc gcatatatta tagtcaaaac ctcaccaggt ttcgctcaag
    68101 gcattaacta ctttatcgat caattaaata ttgaagaaat attaggtacg gtgagcggaa
    68161 atgatactac attaatttta acagcttcga atgaaatggc agaatatgta tatgcaaagt
    68221 tatttagata gtcctgtttt aaatgtatat taacaaacct taataatgtc gtgcgtacac
    68281 aagttagtta ctacatatta acttgtgtac gcgttttttt atggaaagaa agaattcata
    68341 gtcattcatt tgactgtata aaaaacttta tacaacatgt ctttgtgggt gtttttgaat
    68401 aaaaaatgta tattttaacc caaaatacct ttatttatgt ataaaaatcc attattatgt
    68461 attgtataac aaaaagatat gaaattttcg actttcttta tgtgaatata atcacatgta
    68521 agcgtttgaa gattgtctat actctaaatg aattcaaaga taaaaggagg aaatagacat
    68581 gacagatggt ccaattaaag taaatagcga aattggagct ttgaaaactg tgttacttaa
    68641 gcgtcctgga aaagaattag aaaatttagt acctgattat ttagatggat tactatttga
    68701 tgatattcca tatttagaag tagctcaaaa agagcatgac cattttgcac aggtgctaag
    68761 agaagagggt gttgaagtac tttaccttga gaagttagca gctgaaagta ttgaaaatcc
    68821 tcaagtaaga agtgaattta ttgatgatgt attagcagag tctaaaaaaa caatattagg
    68881 tcatgaagaa gaaattaagg cattatttgc gacactttct aatcaagaac ttgtagataa
    68941 aataatgtca ggagtacgta aggaagaaat taatccgaaa tgtacacatc tagtagagta
    69001 tatggatgat aagtatccat tctatttaga tccaatgcca aacctttatt ttactagaga
    69061 tccacaagcc tcaataggac acggtataac aatcaatcgg atgttctgga gagcacgacg
    69121 acgagaatca atatttattc aatatattgt aaagcatcat cctagattta aagatgcgaa
    69181 tattccaatc tggttagatc gagattgccc attcaatatt gaaggcggcg atgaacttgt
    69241 tttatctaaa gaagtcttgg ctataggcgt ttcagaacgt acatctgcac aagctattga
    69301 aaagttagcg cgacgtattt ttgaaaatcc gcaggcgacg tttaaaaaag tagtagcaat
    69361 tgaaattcca actagtcgaa catttatgca cttagataca gtatttacaa tgatagacta
    69421 tgacaaattt acaatgcatt cagccatttt aaaggcagaa ggcaatatga atatatttat
    69481 tattgaatat gatgatgtaa ataaagatat tgccatcaaa caatctagtc atttaaaaga
    69541 tactttagaa gacgttctag gtatagatga tatccaattc attccaacag gaaatggtga
    69601 tgtcattgat ggtgctagag agcaatggaa tgatggctca aatacattat gtataagacc
    69661 aggtgttgta gtgacttacg atagaaacta tgtatcgaat gatttattga gacaaaaagg
    69721 tataaaagtc attgaaatat ctggtagcga gttggtacgt ggacgtgggg gtcctagatg
    69781 tatgagtcaa ccactattca gagaagacat ttaacgtaat aaattataga agcgagggtg
    69841 aaaataatga cagaaattca aaaaccgtat gatttaaaag gcagatcatt attaaaagaa
    69901 agtgatttta ccaaagcaga attcgaagga cttattgatt ttgcaattac attaaaagag
    69961 tataagaaaa acggtattaa gcatcactac ttatctggaa aaaatattgc actactattc
    70021 gaaaagaatt cgacgagaac gcgtgcagcg tttacagttg cgtctattga tttaggtgcg
    70081 catccagaat ttttagggaa aaatgatatt caattaggca aaaaagaatc tgtagaggat
    70141 actgcgaaag tattaggtag aatgttcgat ggtattgaat tccgtggttt ttcacaacaa
    70201 gctgttgaag atttagcgaa gttctctggt gtaccggtgt ggaatggatt aacagacgat
    70261 tggcatccta cacaaatgtt agctgatttt atgacaataa aagagaattt tggatatcta
    70321 gaaggaataa atttaactta cgttggagat ggacgtaata atattgcgca ttcattaatg
    70381 gtagcaggtg ctatgttagg tgttaatgta agaatttgta cacctaaatc attaaatcca
    70441 aaagaggcat atgttgatat tgcaaaagaa aaagcgagtc aatatggtgg ttcagtcatg
    70501 attacggata atattgcaga agcagttgaa aatacagatg ctatatatac agatgtttgg
    70561 gtatcgatgg gtgaagaaag tgaatttgaa caacgtatta atttattaaa agactatcaa
    70621 gtgaatcaac agatgtttga tttaacaggt aaagattcaa cgatattctt acattgttta
    70681 ccagcattcc atgatacaaa tacactttat ggacaagaaa tttatgaaaa atatggatta
    70741 gctgaaatgg aagttacaga ccaaattttt agaagtgaac attcaaaagt gtttgatcaa
    70801 gctgaaaata gaatgcatac aattaaagca gtaatggcag caacattggg gagttaatca
    70861 ctaaatggaa cgatatgaat atgatgtgtc tgatgatata agtgtcatgt acagacacct
    70921 catattgata ttaaaggaga aatgaatatg aacgaatcag gagataacaa actcagtaaa
    70981 tcttctttaa ttggactagt tataggatcc atgattggtg gcggtgcgtt caatataatg
    71041 tctgatatgg gcggtaaagc cggtggatta gccattatta ttggttggat tattacagct
    71101 ataggaatga tttcattagc gttcgtattt caaaatttaa ccaatgaacg gccggagcta
    71161 gacggtggta tttatagtta tgctcaagca ggatttggcg attttgtagg ctttatcagt
    71221 gcttggggat attggttctc agcattttta ggcaatgttg cctatgcaac actattgatg
    71281 tcagcagttg gtaacttttt cccgattttt aaaggaggca acacattacc aagtattatt
    71341 gtcgcctcgt tactactctg gggtgtgcat ttcctaattt taaaaggtgt tgaaacagca
    71401 gcctttatca atagtattgt tactgttgca aagttaatac cgattttact tgtaatcata
    71461 tgcatgataa ttgcattcaa ttttgacact tttaaaacag gctttttcgg tatgacgtca
    71521 gagggtgtat tgccatttag ttgggcgagc acaatgagcc aagttaaaag tacgatgcta
    71581 gtgacagttt gggtgtttat cggtatcgaa ggtgcagtaa ttttttctag cagagctaaa
    71641 aataaaaaag atgtaggtag tgccacggtt ataggactta tatcagtttt aattatctat
    71701 ttcttattaa ctgtattagc tcaaggcgtg attttgcaaa atcatatttc tcaattagat
    71761 tcgccaagta tggcacaggt gcttgcatct attgtaggtg gttggggatc tacacttgta
    71821 aatattggtt taattatttc ggtactaggt gcatggttag gatggacatt gcttgctggt
    71881 gaattacctt tcattgttgc aaaagatgga ttatttccaa aatggtttgc taaagaaaat
    71941 aaaaatggag cacctgtaaa tgcactgctt attaccaata tattagtaca attattttta
    72001 ataagtatgc tatttacaca gagtgcgtat caatttgcat tttcactagc atcaagtgct
    72061 attttatacc cttacatgtt cagtgcattt tatcaagtta aatacacgtt agagcaccga
    72121 cagcaagcaa ctactaaaca atggacgatt ggtatcatag cctcaattta tgctatatgg
    72181 cttatatatg cagcaggtat caattactta ttattgacga tgttacttta tattccagct
    72241 cttcttgttt attcaatcgt tcaaaagaat aatcagacac gtttgattaa atcagactat
    72301 attcttttta tgattattat cgtacttgca gttatcgggt taattaagtt attgatggga
    72361 acgataaatg ttttttaaaa ggagcgacaa aaatatgaaa gagaaaattg tcattgcatt
    72421 aggcggtaat gcgatacaga caaaagaagc aacagctgaa gcacaacaaa cagctattag
    72481 acgtgcgatg caaaacctta aacctttatt tgattcacca gcgcgtattg tcatttcaca
    72541 tggtaatggc ccacaaattg gaagtttatt aatccaacaa gctaaatcga acagtgacac
    72601 aacgccggca atgccattgg atacttgtgg tgcaatgtca cagggtatga taggctattg
    72661 gttggaaact gaaatcaatc gcattttaac tgaaatgaat agtgatagaa ctgtaggcac
    72721 aatcgttaca cgtgtggaag tagataaaga tgatccacga tttgataacc caactaaacc
    72781 aattggtcct ttttatacga aagaagaagt tgaagaatta caaaaagaac agccagactc
    72841 agtctttaaa gaagatgcag gacgtggtta tagaaaagta gttgcgtcac cactacctca
    72901 atctatacta gaacaccagt taattcgaac tttagcagac ggtaaaaata ttgtcattgc
    72961 atgcggtggt ggcggtattc cagttataaa aaaagaaaat acctatgaag gtgttgaagc
    73021 ggttatagat aaagattttg ctagtgagaa attagcaacg ctgattgaag cagatacctt
    73081 aatgattctt acgaatgtag aaaatgtatt tattaacttt aatgaaccta atcaacaaca
    73141 aatcgatgat attgatgtag caacactgaa aaaatacgcg gcacaaggta agtttgcgga
    73201 aggatcgatg ttgccaaaaa tagaagctgc gatacgattt gttgaaagtg gggaaaacaa
    73261 aaaagttatc attaccaatt tagagcaggc atacgaagct ttgattggta ataaaggtac
    73321 acacattcac atgtagcact ttatcacgcg acaaaacatt aaatatgttt ctccgttgat
    73381 tcaaatgaaa aagttgtctg ctgacacttt gcaaggtttg aaggagttta acttatgaca
    73441 gaaaacttta ttttgggtag aaataataaa ttagaacatg aactaaaggc attagcagat
    73501 tacattaata tcccatatag tatattacaa ccatatcaaa gtgaatgttt tgtcagacat
    73561 tatacaaaag gccaagttat ttatttttcg ccgcaagaaa gtagcaatat ttacttttta
    73621 attgaaggta acattattag agaacattac aatcaaaatg gagatgtata tcgttatttt
    73681 aataaagagc aagtattatt tccaatcagt aacttatttc atccgaaaga ggttaacgaa
    73741 ttgtgcacag cattaaccga ttgtacagtt cttggattgc ctagagaatt gatggccttt
    73801 ttgtgcaaag ctaatgatga tatatttttg acactttttg cattaataaa tgataatgag
    73861 cagcaacaca tgaactataa catggcatta acaagtaaat ttgctaaaga tcgaattatc
    73921 aaattgttat gccatctatg tcagacagta ggatacgatc aagatgaatt ttatgaaatc
    73981 aaacagtttt taactattca actcatgagt gatatggctg ggatttcccg ggaaacagct
    74041 ggtcatatta ttcatgaact taaagatgaa aaacttgttg ttaaagatca taaaaattgg
    74101 ttagtaagca aacatttatt caatgatgta tgtgtttaat atacaatgta aaatgaataa
    74161 gttgaacatg aggtctaacg tacatttata cgttaggcct tttttgctag catgatgatt
    74221 aatttaatat gttagttaaa tttgattgtt gaaattacag taaaatttaa ggtaatgaaa
    74281 aatttagaac ttctaagttt ttgaaaagta aaacatttgc aatagtgtaa aaatagtata
    74341 ttgatttttg ctagttaaca gaaaatttta agttatataa ataggaagaa aacaaatttt
    74401 acgtaatttt tttcgaaaag caattgatat aattcttatt tcattataca atttagacta
    74461 atctagaaat tgaaatggag taatattttt gaaaaaaaga attgattatt tgtcgaataa
    74521 gcagaataag tattcgatta gacgttttac agtaggtacc acatcagtaa tagtaggggc
    74581 aactatacta tttgggatag gcaatcatca agcacaagct tcagaacaat cgaacgatac
    74641 aacgcaatct tcgaaaaata atgcaagtgc agattccgaa aaaaacaata tgatagaaac
    74701 acctcaatta aatacaacgg ctaatgatac atctgatatt agtgcaaaca caaacagtgc
    74761 gaatgtagat agcacagcaa aaccaatgtc tacacaaacg agcaatacca ctacaacaga
    74821 gccagcttca acaaatgaaa cacctcaacc gacggcaatt aaagatcaag caactgctgc
    74881 aaaaatgcaa gatcaaactg ttcctcaaga agcaaattct caagtagata ataaaacaac
    74941 gaaagatgct aatagcatag caacaaacag tgagcttaaa aatcctcaaa cattagattt
    75001 accacaatca tcaccacaaa caatttccaa tgcgcaagga actagtaaac caagtgttag
    75061 aacgagagct gtacgtagtc ttacagttgc tgaacctgta gtaaatgctg ctgatgctaa
    75121 aggtacaaat gtaaatgata aagttacggc aagtaatcta cagttgcaaa agactacatt
    75181 tgatcctaac caaagtggta acacatttat ggcggcaaat tttacagtga cagataaagt
    75241 gaaatcaggg gattatttta cagcgaagtt accagatagt ttaactggta atggagatgt
    75301 ggattactct aactcgaata atacgatgcc aattgcagat attaaaagta cgaatggcga
    75361 tgttgtagct aaagcaacat atgatatctt gactaagacg tatacatttg tctttacaga
    75421 ttatgtaaat gataaagaaa atattaacgg acaattttca ttaccattat ttacagaccg
    75481 agcaaaggca cctaaatcag gaacatatga tgcaaatatt aatattgcgg atgaaatgtt
    75541 tgataataaa attacttata actatagttc accaattgca ggaattgata agccaaatgg
    75601 cgcgaacatt tcttctcaaa ttattggtgt agatacagct tcaggccaaa acacatacaa
    75661 gcaaacggtc tttgttaacc ctaagcaacg agttttaggt aatacgtggg tgtatattaa
    75721 aggttaccaa gataaaatcg aagaaagtag cggtaaagta agtgctacag atacaaaact
    75781 gagaattttt gaagtgaatg atacatctaa attatcagat agctactatg cagacccaaa
    75841 tgactctaac cttaaagaag taactggtga gtttaataat agaatttttt atgaacatcc
    75901 aaacgtagca agtattaatt ttggtgatat aaataaaacg tatgtcgttt tagttgaagg
    75961 tcattatgat aatactggta aaaacttgaa aacacaggtt attcaagaaa atattgaccc
    76021 agcgacaggt aaagactaca gtattttcgg ttggaataat gagaatgttg tacgttatgg
    76081 aggcggaagt gctgatggtg attcagcagt aaatccagtt gatccaacac cgggaccacc
    76141 agttgaccca ga
//
LOCUS       DAPIDM010000002        72651 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000002 DAPIDM010000000
VERSION     DAPIDM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 72651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.002"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..534)
                     /gene="srtB"
                     /locus_tag="RJE42_000061"
     CDS             complement(<1..534)
                     /gene="srtB"
                     /locus_tag="RJE42_000061"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDV5875523.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTK"
     gene            complement(596..1561)
                     /gene="isdF"
                     /locus_tag="RJE42_000062"
     CDS             complement(596..1561)
                     /gene="isdF"
                     /locus_tag="RJE42_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDV5875524.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTTKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDVQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(1574..2452)
                     /gene="isdE"
                     /locus_tag="RJE42_000063"
     CDS             complement(1574..2452)
                     /gene="isdE"
                     /locus_tag="RJE42_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDV5875525.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(2449..3525)
                     /gene="isdD"
                     /locus_tag="RJE42_000064"
     CDS             complement(2449..3525)
                     /gene="isdD"
                     /locus_tag="RJE42_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDV5875526.1"
                     /translation="MRNVKQIATKSIIAIISLGMLTYTTMIGSVLADEINYPSAKFNQ
                     PEAKDKTELTSSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDYTIVRILIEHHMDKINMHVKTLTEK
                     KDLDNKEMVYSIHFKEKKVQHNDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSDKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(3522..4208)
                     /gene="isdC"
                     /locus_tag="RJE42_000065"
     CDS             complement(3522..4208)
                     /gene="isdC"
                     /locus_tag="RJE42_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDV5875527.1"
                     /translation="MKNILKVFNTMILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKEKIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGATTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVEQ"
     gene            4417..5481
                     /gene="isdA"
                     /locus_tag="RJE42_000066"
     CDS             4417..5481
                     /gene="isdA"
                     /locus_tag="RJE42_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDV5875528.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQAVLNNASFWKEYKFYNANNQELATTVVNDDKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPGQPKTPTEQTKPVQPKVEK
                     VKPAVTAPSKAENTQTTKVVSTEATKDQSQTQSARTVKTTQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKQNEVHKQGPSKDSKAKELPKTGLTSVDNFISTVAF
                     ATLALLGSLSLLLFKRKESK"
     gene            5684..7603
                     /gene="isdB"
                     /locus_tag="RJE42_000067"
     CDS             5684..7603
                     /gene="isdB"
                     /locus_tag="RJE42_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDV5875529.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAKAAEET
                     GGTNTEAQPKTEAVASPTTTTTEKAPEAKPVANAVSVANKEVVAPTTETKEVKAPKEA
                     KEEKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDGTQQFYH
                     YASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIR
                     FSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLAPY
                     KKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEETKKALDEQVKSAITEFQNVQPTNE
                     KMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVE
                     GQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTK
                     ANADKSNKKEQQDNSAKKETTPATPSKPTTPPVEKESQKQDSQKDDNKQSASVEKEND
                     ASSESAKDKTPATKPAKAEVESSSTTPTKVVSTTQNVAKPTTASSETTKDVVQTSAGS
                     SEAKDSAPLQKANIKNTNDGDTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLA
                     LSSIVAFVLPRKRKN"
     gene            complement(7757..7930)
                     /gene="rpmF"
                     /locus_tag="RJE42_000068"
     CDS             complement(7757..7930)
                     /gene="rpmF"
                     /locus_tag="RJE42_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV5875530.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(8010..8567)
                     /locus_tag="RJE42_000069"
     CDS             complement(8010..8567)
                     /locus_tag="RJE42_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDV5875531.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDGQDDVDE
                     HYHIINDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            8694..9833
                     /locus_tag="RJE42_000070"
     CDS             8694..9833
                     /locus_tag="RJE42_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDV5875532.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHQYASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSNINEATDFHHFV
                     KSLKTKRYTYTHIQRLLMNILLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYC"
     gene            complement(9895..10377)
                     /gene="coaD"
                     /locus_tag="RJE42_000071"
     CDS             complement(9895..10377)
                     /gene="coaD"
                     /locus_tag="RJE42_000071"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV5875533.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(10379..10921)
                     /gene="rsmD"
                     /locus_tag="RJE42_000072"
     CDS             complement(10379..10921)
                     /gene="rsmD"
                     /locus_tag="RJE42_000072"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDV5875534.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLNLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            10991..11380
                     /locus_tag="RJE42_000073"
     CDS             10991..11380
                     /locus_tag="RJE42_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875535.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(11383..11637)
                     /locus_tag="RJE42_000074"
     CDS             complement(11383..11637)
                     /locus_tag="RJE42_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDV5875536.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            11876..12805
                     /locus_tag="RJE42_000075"
     CDS             11876..12805
                     /locus_tag="RJE42_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV5875537.1"
                     /translation="MKKLNRNILNAVLVGAGFVSSLLMITKNKVITKKQTIPDFFKGN
                     APYIFAHRGGMTLRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAGYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGINLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIKNKQ"
     gene            complement(12872..13306)
                     /locus_tag="RJE42_000076"
     CDS             complement(12872..13306)
                     /locus_tag="RJE42_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDV5875538.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(13322..14359)
                     /locus_tag="RJE42_000077"
     CDS             complement(13322..14359)
                     /locus_tag="RJE42_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDV5875539.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGNIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYRLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(14686..15147)
                     /locus_tag="RJE42_000078"
     CDS             complement(14686..15147)
                     /locus_tag="RJE42_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDV5875540.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(15172..16083)
                     /locus_tag="RJE42_000079"
     CDS             complement(15172..16083)
                     /locus_tag="RJE42_000079"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV5875541.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            16534..17445
                     /locus_tag="RJE42_000080"
     CDS             16534..17445
                     /locus_tag="RJE42_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDV5875542.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(17592..21044)
                     /locus_tag="RJE42_000081"
     CDS             complement(17592..21044)
                     /locus_tag="RJE42_000081"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDV5875543.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSDLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRTAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKAAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQSPFDGMIKQVTVNNGDTIATGDLLIEIE
                     KASD"
     gene            complement(21597..22823)
                     /gene="ftsW"
                     /locus_tag="RJE42_000082"
     CDS             complement(21597..22823)
                     /gene="ftsW"
                     /locus_tag="RJE42_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV5875544.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(23137..23412)
                     /locus_tag="RJE42_000083"
     CDS             complement(23137..23412)
                     /locus_tag="RJE42_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDV5875545.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            23553..24035
                     /locus_tag="RJE42_000084"
     CDS             23553..24035
                     /locus_tag="RJE42_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875546.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            24037..24204
                     /locus_tag="RJE42_000085"
     CDS             24037..24204
                     /locus_tag="RJE42_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDV5875547.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(24343..26190)
                     /gene="typA"
                     /locus_tag="RJE42_000086"
     CDS             complement(24343..26190)
                     /gene="typA"
                     /locus_tag="RJE42_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDV5875548.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            26292..26483
                     /locus_tag="RJE42_000087"
     CDS             26292..26483
                     /locus_tag="RJE42_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDV5875549.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(26637..27464)
                     /locus_tag="RJE42_000088"
     CDS             complement(26637..27464)
                     /locus_tag="RJE42_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDV5875550.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHRDALIQLHEQRFKRKSK"
     gene            27621..28235
                     /locus_tag="RJE42_000089"
     CDS             27621..28235
                     /locus_tag="RJE42_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDV5875551.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            28417..29769
                     /locus_tag="RJE42_000090"
     CDS             28417..29769
                     /locus_tag="RJE42_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV5875552.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQG
                     "
     gene            complement(29851..30270)
                     /locus_tag="RJE42_000091"
     CDS             complement(29851..30270)
                     /locus_tag="RJE42_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDV5875553.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(30553..31572)
                     /locus_tag="RJE42_000092"
     CDS             complement(30553..31572)
                     /locus_tag="RJE42_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDV5875554.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNDYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     VEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(31646..32719)
                     /locus_tag="RJE42_000093"
     CDS             complement(31646..32719)
                     /locus_tag="RJE42_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV5875555.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDSELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLEAKNNKQNTEFVGYATPNKAARQLLPKEVKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(32719..33528)
                     /locus_tag="RJE42_000094"
     CDS             complement(32719..33528)
                     /locus_tag="RJE42_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875556.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(33534..34331)
                     /locus_tag="RJE42_000095"
     CDS             complement(33534..34331)
                     /locus_tag="RJE42_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875557.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(34324..35418)
                     /locus_tag="RJE42_000096"
     CDS             complement(34324..35418)
                     /locus_tag="RJE42_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875558.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGYLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDHA"
     gene            complement(35431..35970)
                     /locus_tag="RJE42_000097"
     CDS             complement(35431..35970)
                     /locus_tag="RJE42_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5875559.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEVYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(36114..36389)
                     /locus_tag="RJE42_000098"
     CDS             complement(36114..36389)
                     /locus_tag="RJE42_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDV5875560.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMDAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKNHSEEQRVTAKK"
     gene            complement(36558..37964)
                     /gene="lpdA"
                     /locus_tag="RJE42_000099"
     CDS             complement(36558..37964)
                     /gene="lpdA"
                     /locus_tag="RJE42_000099"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV5875561.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(37968..39260)
                     /locus_tag="RJE42_000100"
     CDS             complement(37968..39260)
                     /locus_tag="RJE42_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDV5875562.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASASNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(39351..40328)
                     /locus_tag="RJE42_000101"
     CDS             complement(39351..40328)
                     /locus_tag="RJE42_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDV5875563.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(40332..41444)
                     /gene="pdhA"
                     /locus_tag="RJE42_000102"
     CDS             complement(40332..41444)
                     /gene="pdhA"
                     /locus_tag="RJE42_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDV5875564.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQTAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(41615..42241)
                     /locus_tag="RJE42_000103"
     CDS             complement(41615..42241)
                     /locus_tag="RJE42_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDV5875565.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            42606..43157
                     /locus_tag="RJE42_000104"
     CDS             42606..43157
                     /locus_tag="RJE42_000104"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV5875566.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPFTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            43488..43610
                     /locus_tag="RJE42_000105"
     CDS             43488..43610
                     /locus_tag="RJE42_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875567.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            43639..43857
                     /locus_tag="RJE42_000106"
     CDS             43639..43857
                     /locus_tag="RJE42_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDV5875568.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            43857..45554
                     /locus_tag="RJE42_000107"
     CDS             43857..45554
                     /locus_tag="RJE42_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDV5875569.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESKNK"
     gene            complement(45903..46565)
                     /locus_tag="RJE42_000108"
     CDS             complement(45903..46565)
                     /locus_tag="RJE42_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDV5875570.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(46698..47717)
                     /locus_tag="RJE42_000109"
     CDS             complement(46698..47717)
                     /locus_tag="RJE42_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV5875571.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMFMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWLFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            complement(47714..49075)
                     /locus_tag="RJE42_000110"
     CDS             complement(47714..49075)
                     /locus_tag="RJE42_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV5875572.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            49273..49506
                     /gene="nrdH"
                     /locus_tag="RJE42_000111"
     CDS             49273..49506
                     /gene="nrdH"
                     /locus_tag="RJE42_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV5875573.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNERQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            complement(49744..51462)
                     /gene="ptsP"
                     /locus_tag="RJE42_000112"
     CDS             complement(49744..51462)
                     /gene="ptsP"
                     /locus_tag="RJE42_000112"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV5875574.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNNAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ASALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(51465..51731)
                     /locus_tag="RJE42_000113"
     CDS             complement(51465..51731)
                     /locus_tag="RJE42_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDV5875575.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(51885..52427)
                     /locus_tag="RJE42_000114"
     CDS             complement(51885..52427)
                     /locus_tag="RJE42_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDV5875576.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRER
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(52481..53653)
                     /locus_tag="RJE42_000115"
     CDS             complement(52481..53653)
                     /locus_tag="RJE42_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDV5875577.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEVNTAFFVKLFNTALAERDYYFNIDRTNAFRL
                     FNAEGDGIGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYATRHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLITGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            complement(54078..55373)
                     /locus_tag="RJE42_000116"
     CDS             complement(54078..55373)
                     /locus_tag="RJE42_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875578.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHY
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTCKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            55525..55659
                     /gene="graF"
                     /locus_tag="RJE42_000117"
     CDS             55525..55659
                     /gene="graF"
                     /locus_tag="RJE42_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDV5875579.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     regulatory      56003..56103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            56180..56755
                     /locus_tag="RJE42_000118"
     CDS             56180..56755
                     /locus_tag="RJE42_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV5875580.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     gene            56770..58170
                     /locus_tag="RJE42_000119"
     CDS             56770..58170
                     /locus_tag="RJE42_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875581.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            58163..58969
                     /locus_tag="RJE42_000120"
     CDS             58163..58969
                     /locus_tag="RJE42_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV5875582.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMFYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(59235..60482)
                     /locus_tag="RJE42_000121"
     CDS             complement(59235..60482)
                     /locus_tag="RJE42_000121"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV5875583.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCEFPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNSELAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDDGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRYDIANKALQLK"
     gene            complement(60504..61982)
                     /gene="purH"
                     /locus_tag="RJE42_000122"
     CDS             complement(60504..61982)
                     /gene="purH"
                     /locus_tag="RJE42_000122"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV5875584.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALNILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            complement(61997..62563)
                     /gene="purN"
                     /locus_tag="RJE42_000123"
     CDS             complement(61997..62563)
                     /gene="purN"
                     /locus_tag="RJE42_000123"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV5875585.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLDSF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            complement(62566..63594)
                     /locus_tag="RJE42_000124"
     CDS             complement(62566..63594)
                     /locus_tag="RJE42_000124"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV5875586.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIETNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            complement(63587..65071)
                     /locus_tag="RJE42_000125"
     CDS             complement(63587..65071)
                     /locus_tag="RJE42_000125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV5875587.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKIKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKM
                     LAQESPVKADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            complement(65050..67239)
                     /gene="purL"
                     /locus_tag="RJE42_000126"
     CDS             complement(65050..67239)
                     /gene="purL"
                     /locus_tag="RJE42_000126"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDV5875588.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYEDEVYADIPVEPLADEAPVYILEGEEKNYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLADSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPIVGMVGLIENVDYLNDF
                     QPQVGDKLYVIGDTKDDFGGSQLEKLIYGKVNHEFESIDLSSEVKKGESIKTAIRQGV
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSLIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            complement(67232..67903)
                     /gene="purQ"
                     /locus_tag="RJE42_000127"
     CDS             complement(67232..67903)
                     /gene="purQ"
                     /locus_tag="RJE42_000127"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDV5875589.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            complement(67905..68168)
                     /gene="purS"
                     /locus_tag="RJE42_000128"
     CDS             complement(67905..68168)
                     /gene="purS"
                     /locus_tag="RJE42_000128"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDV5875590.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLEDEKENA"
     gene            complement(68168..68872)
                     /locus_tag="RJE42_000129"
     CDS             complement(68168..68872)
                     /locus_tag="RJE42_000129"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV5875591.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIATDKDIETLKTKALEINQVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            complement(68876..70000)
                     /gene="purK"
                     /locus_tag="RJE42_000130"
     CDS             complement(68876..70000)
                     /gene="purK"
                     /locus_tag="RJE42_000130"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV5875592.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(69987..70469)
                     /gene="purE"
                     /locus_tag="RJE42_000131"
     CDS             complement(69987..70469)
                     /gene="purE"
                     /locus_tag="RJE42_000131"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV5875593.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILATRMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            70670..71530
                     /gene="folD"
                     /locus_tag="RJE42_000132"
     CDS             70670..71530
                     /gene="folD"
                     /locus_tag="RJE42_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV5875594.1"
                     /translation="MVAKILDGKQIAKDYRQGLQNQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            72339..>72651
                     /locus_tag="RJE42_000133"
     CDS             72339..>72651
                     /locus_tag="RJE42_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDV5875595.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EV"
BASE COUNT        23944 a        13219 c        10397 g        25091 t
ORIGIN      
        1 tttagtagta gttttatatg cactaaatac ttgcaattga tatttaccat atttattgtc
       61 aaattcaatt atcttgtgtt tttcataaaa cgattgcttt aaataatctt ctaacacatc
      121 aaacatcgta ttatcaccga catggtgccc gtataaaata gtattatgat ttaaattctt
      181 caattcattt ctaaaatcca taaaaatact acctttacgt cgatgttctc gctcaaaatc
      241 taaatttaaa taatcgtgat ttgtcttacc ttgtagtact ggataattta atgatgttcc
      301 tgataatttt atccatccaa caatgtcttt atttattttt tcaagtgatt caaattgtgg
      361 tctcacatgt gcttgatgtt tgctcatcag catttgaaat ttttgttgta atttctcata
      421 atttgcgcgt tcttgcttgt cttcaatata tgtttgaaca attttgtaac caaaaatgat
      481 aataattaca accaataaaa tttgtacaat agttaaaaat cgtttcattc tcataaaaat
      541 cctcttttat taacgacgtt tcttcagtca tcactaaacc agttgttgta ccgttttaga
      601 ttcgatttcg ttgactttga caaattaagt aaattagcat tggaccaccg acaatcatta
      661 aaatagcatt ggctggaatt tctaaaggag gctgtatcac tcgtcctaat aaatcagcca
      721 ctaacaatag ccatgcacca ataactgtag aaaacggaat aagtactctg taattgcccc
      781 caactagctt tctaaccaca tgtggcacaa taatacctaa aaaggctagt tgtccaacaa
      841 tcgcaacagt tgcacttgct aaaaatacag ctaataaacc tgttaaccat ctgtaacgat
      901 caatattaaa accgatgctt cgcgcttgta cgtcatctaa atttagtaaa ttcaatttag
      961 gggacaatag taatgttaat attaatccca ataatgctga tactgctaat atgtatacgt
     1021 cgctccatat tttcattgtt aagccttgag gaattttcat taaagggttt tgagttaaaa
     1081 tttctaaaac accatttaat aatacgaata acgcaacacc tactaatatc atactaacag
     1141 cattgaatct aaatttagaa tgcaacaata taattattaa aaatggtatt aaacctccaa
     1201 taaaacttaa taatggtaag taaaagtaca attgtggaat aaacaacata caaagtgctc
     1261 tcattataag tgcacctgat gaaacgccaa tgatattcgc ctctgccaaa ggattttgta
     1321 gtgctgcttg taataatgct ccagaaactg ctaacattgc gccaaccatc aatgcaatta
     1381 atatacgtgg caatcgcaaa tcaataattg aatccactgc ttcattgcta ccagttgtaa
     1441 attttgtaaa taggtcatta aatgacaatt tagttgtacc ggttacaaac gaaatataag
     1501 ccgttgcgat taaaatgact aacaaacata aaaatagtag tttcttttta ttttttatca
     1561 tcatatcaac tcactatttt ttatccttat aaaataaatc atataattga gtcattgcct
     1621 tatcagcatc aacatttgct gtaataccga atggcacttc ctctaagtca taaacatgat
     1681 tatttttcac agctttaaaa tgtttccaaa tatcattttg tttaaattct ttttgaaaca
     1741 ttttcttaac ttcttcaggc attccgtgag gtaatcgtaa aataatatct ggattgatat
     1801 tcaacaaatt ttcagtatta gacgaaatat attgacgatc tttcacttta ataacatttt
     1861 ctccacctgc tattttaact aaatcaccaa tatatgattt atcagttgct actaaatagc
     1921 taccaggtac acccattaat attaatactt tgggatgttt cttttgttta gctgctttat
     1981 tttcaatttt ttgctttaca gaatttaaat ggtcatttaa ttcttttgct tgtgctttac
     2041 gattaaattg atcacctaat tgtgtaatcg acttttgcat cccttttaaa ctatcaaaat
     2101 cataaaagta acctttcata tttaattgtt tgtaaaatgg ttgcatttca tctttaatcg
     2161 ttgacacact caaaacatgt gttggtttta actttttaac agcttcaaca ttcggctcca
     2221 ttggttgacc aatttccggt acatctttat aacgattagg caatgtctta tatgacgtgg
     2281 gtttgccgac aattggtaaa tccaatttgt ccaatgtcat tgtcaatgca acagttgttg
     2341 gtacgattct gaattcgccg gatttagttg attcttgaga actggaagat tgacagctag
     2401 ttaagataac gacgcttatc actaaaatgg ttaaatactt tatgattctc aaacttggtt
     2461 acctctcttt ctgctgtata aatgtgccga aataagtaaa atggttgcta acacaatgcc
     2521 agctgcaaat aacatatatt gctttttatg atttggttca tcaagttctt taatatcact
     2581 agataattga ctactattct tgctatcatc atctctatta aaagctggta acgctttttc
     2641 tttttcaact acttttttct tttccttttt tgatttttca tctttatgtt tcggttgctc
     2701 tttgtgatta ttttcaacct tttttactgt tggagggcta ttttcaactt ttaatgtacc
     2761 tgatgcttta ggtcgctcat tttcaatctt tttatcactg tcttgttgca ctttatcttt
     2821 ttgtacagga atatcattta atcctttttc agtaaaaagg ctagtacgtc tttccttaat
     2881 gtctgatttt tgactatctt ttttgtcatc aatatctttt ttaagctgat cttgattatt
     2941 ttcttgattt tgatgttttg aaggcacttc ttttgcatca ttatgttgta cttttttctc
     3001 tttaaaatga atagaataca ccatttcttt gttatctaga tctttctttt cagtcaacgt
     3061 tttaacatgc atattgattt tatccatatg atgttcaatt aaaattctta ctatcgtata
     3121 atccttttca tcttttataa caaaaggaaa ttcttcattt ttgtcatttt gaaaaacaag
     3181 ttgctcttta tcaatttgac cttttagttt tacttgcaag taaattttac cagtcatatc
     3241 tgaaatcaat tgcgcctttt catcaactaa ttgttgttcc tcagttacat ttttattttc
     3301 ttgattaaaa attagtaatt ctaacgcttt attctctttt atcttttcat caaaaattga
     3361 tgaagttaat tctgttttat cttttgcttc aggttgatta aatttggctg atggatagtt
     3421 tatctcatca gccaacacgc taccaatcat tgttgtatat gtaagcatac ctaagctaat
     3481 aatagctata atagattttg tagcaatttg tttaacattt ctcattgttc cacattgcct
     3541 ttagattttt ttctaacaaa caatgtgatt gcgattaata atgctaagga tgcaactggt
     3601 attgcatata tatatgcagg tgtaccagca tttgtttgtg gattttctac tttgtcttta
     3661 ttcgaactgt tagattcact ttgaccagta gtcgctccat cagatccttt gtcactacct
     3721 gaagcagaat ttttatcatc tttacctggt gcattagcac ctgctacatc agttggtcca
     3781 ttaaatttat atgtaatatt gtaatgatgg tcatatttga aaggttttcc gttcactttt
     3841 tcatcgatat aaacatcaat tttcccatct attttaccgt ttagcttact tacttcaaat
     3901 tcagaagttc gttcatcttt ggcagtattt ttactaataa ttttttcttt atgtccttct
     3961 atactcattc ctgtaatcca atgactgtgg tttacagtta tttgaacata caatttacca
     4021 tttttcttaa tgtactttgc cggtttatta aaatagtcat tagcaatcga cgtgtcattg
     4081 gtattgtatt tgtaaacctc ataattcaaa gtaccgctat ctgcggcatt tgcagaatta
     4141 ctgaatgtcg cgatgatgat aattaacgct aaaatcattg tattaaaaac ttttaaaata
     4201 tttttcacaa tataatcctc ctttttatga ttgcttttaa gtctttacta aaatcataaa
     4261 taataatgat tatcattgtc aatatttatt ttataatcaa tttattattg ttatacgaaa
     4321 atagatgtgc tagtataatt gataaccatt atcaattgca atggttaatc atctcatata
     4381 acaacacata atttgtatcc ttaggaggaa aacaacatga caaaacatta tttaaacagt
     4441 aagtatcaat cagaacaacg ttcatcagct atgaaaaaga ttacaatggg tacagcatct
     4501 atcattttag gttcccttgt atacataggc gcagacagcc aacaagttaa tgcggcaaca
     4561 gaagctacga acgcaactaa taatcaaagc acacaagttt ctcaagcaac atcacaacca
     4621 attaatttcc aagtgcagaa agatggctct tcagagaagt cacacatgga tgactatatg
     4681 caacaccctg gtaaagtaat taaacaaaat aataaatatt atttccaagc tgtattgaac
     4741 aacgcatcat tctggaaaga atacaaattt tacaatgcaa acaatcaaga attagcaaca
     4801 actgttgtta acgatgataa aaaagctgac actagaacaa tcaacgttgc tgttgaacct
     4861 gggtataaga gtttaactac aaaagtacat attgtcgtac ctcaaattaa ttacaatcat
     4921 agatatacta cgcatttaga atttgaaaaa gcaattccta cattagctga cgcagcaaaa
     4981 ccaaacaatg ttaaaccggt tcaaccaaaa cctggtcaac ctaaaacacc tactgagcaa
     5041 acgaaaccag ttcaacctaa agttgaaaaa gttaagcctg ctgtaactgc accaagcaaa
     5101 gctgaaaaca cacaaactac aaaagttgta agtactgaag ctacaaaaga tcaaagtcaa
     5161 acacaaagtg ctcgtacagt gaaaacaaca caaacagctc aagaacaaaa taaagttcaa
     5221 acacctgtta aagatgttgc aacagcgaaa tctgaaagca acaatcaagc tgtaagtgac
     5281 aataaatcac aacaaactaa caaagttaca aaacaaaacg aagttcataa acaaggacct
     5341 tcaaaagatt ctaaagctaa agaattacca aaaactggtt taacttcagt tgataacttt
     5401 attagcacag ttgccttcgc aacacttgcc cttttaggtt cattatcttt gttacttttc
     5461 aaaagaaaag aatctaaata aatcttcgtc acactcataa cttaatatat tttttatttt
     5521 aaattttatt taacctatgt tatagatatt ccataatcta taacataggt tattttttta
     5581 taaaataacg ttgcaattaa ctaccatttc aatgtacaat acaagtaatc aattgataat
     5641 gattatcagt tgataatata caattaggag ttgtttctac aacatgaaca aacagcaaaa
     5701 agaatttaaa tcattttatt caattagaaa gtcatcacta ggcgttgcat ctgtagcgat
     5761 tagtacactt ttattattaa tgtcaaatgg cgaagcaaaa gcagctgaag aaacaggtgg
     5821 tacaaataca gaagcacaac caaaaactga agcagttgca agtccaacaa caacaacaac
     5881 tgaaaaagct ccagaagcta aaccagtagc taatgctgtc tcagtagcta ataaagaagt
     5941 tgtggcccct actactgaaa caaaagaagt taaagcccct aaggaagcaa aagaggaaaa
     6001 acctgcagca aaagctgaca acaatacata tcctattttg aatcaggaac taagagaagc
     6061 gattaaaaac ccagcaataa aagataaaga tcatagcgca ccaaactctc gtccaattga
     6121 ttttgaaatg aaaaagaaag atggcactca acaattttat cattatgcaa gctctgttaa
     6181 accagctaga gttattttca ctgattcaaa accagaaata gaattaggtt tacaatcagg
     6241 gcaattttgg agaaaatttg aagtttatga aggtgacaaa aagttgccaa ttaaattagt
     6301 atcatacgat actgttaaag attatgctta cattcgcttc tctgtttcaa atggaacaaa
     6361 agcagttaaa atcgtaagtt caactcactt caataacaaa gaagaaaaat acgattacac
     6421 attaatggaa ttcgcacaac caatttataa cagtgcagat aaattcaaaa ctgaagaaga
     6481 ttataaagct gaaaaattat tagcgccata taaaaaagcg aaaacactag aaagacaagt
     6541 ttatgaatta aataaaattc aagataaact tcctgaaaaa ttgaaagctg aatacaagaa
     6601 gaaattagaa gagacgaaaa aagctttaga tgaacaagtg aaatccgcga taactgaatt
     6661 ccaaaatgta caaccaacaa atgaaaaaat gaccgattta caagatacaa aatatgttgt
     6721 ttatgaaagt gttgagaata acgaatctat gatggatact tttgttaaac accctattaa
     6781 aacaggtatg cttaacggca aaaaatatat ggtaatggaa actactaatg acgattactg
     6841 gaaagatttc atggttgaag gtcaacgtgt tagaacaatt agcaaagatg ctaaaaataa
     6901 cactagaacg attattttcc catatgttga aggtaaaact ctatatgatg ctatcgttaa
     6961 agttcacgta aaaacgattg attatgatgg acaataccat gtcagaatcg ttgataaaga
     7021 agcatttaca aaagccaatg ccgataaatc taataaaaag gaacaacaag acaactcagc
     7081 taagaaggaa actactccag ctacgcctag caaaccaaca acaccacctg ttgaaaaaga
     7141 atcacaaaaa caagacagcc aaaaagatga caataaacaa tcagcaagtg ttgaaaaaga
     7201 aaatgacgca tctagtgagt cagctaaaga caaaacgcct gctacaaaac cagctaaagc
     7261 tgaagtagaa tcaagtagta caactccaac taaggtagta tctacgactc aaaatgttgc
     7321 aaaaccaaca actgcttcat cagaaacaac aaaagatgtt gttcaaactt cagcaggttc
     7381 tagcgaagca aaagatagtg ctccattaca aaaagcaaac attaaaaaca caaatgatgg
     7441 agacactcaa agccaaaaca ataaaaatac acaagaaaat aaagcaaaat cattaccaca
     7501 aactggtgaa gaatcaaata aagatatgac attaccatta atggcattat tagctttaag
     7561 tagtatcgtt gcattcgtat tacctagaaa acgtaaaaac taattaatcg tctttatatt
     7621 taattattaa attaacaaat tttaattggc agatgaggta ttccgatacc tcgtccgcca
     7681 attatttttt gcaatataaa aagtcccact taaaacaatt attttaagcg ggacttttta
     7741 tattgagtaa ctaaaattat ttagctgcta cttcttcacc attgtaagaa ccacagtttt
     7801 tacatacacg gtgtgataat ttgtattcgc cacagtttgg gcattcagtc atacctggta
     7861 ctgaaatttt gaaatgcgta cgacgtttgt tttttctagt tttagaagtt cttctttttg
     7921 gtactgccat gatatatcct ccttagatta taaacgaaaa atactaaatg ttagtttaat
     7981 taacaacatt atatcattaa ttaaactact tattgctctt tatcatataa ttgttgtaat
     8041 ttttgaagcc ttggatcaac ttgtcgtgat tctgaatcat cttgttcttg ctgtttagca
     8101 agctcatcta attgatcctc atcgattact tcccaaccat tacctactgt caacatttgg
     8161 tcactttgct ctgaataagc tctcattggt ttctcaataa taactatatc ctcgacaata
     8221 tcctgaagat taaccatacc atcgttaata atgtgatagt gttcatctac atcatcttga
     8281 ccatcgttat actgattgta cccttctaaa tcaaatactt ctgtagtagt tacatctagt
     8341 gggactttaa ctggtacaag agtacgtgca caaggcattg tatacgttcc agtaatgtga
     8401 atatccgcaa cgacttctgt tgacttaatg gttaactgac cttggattgt aattggagat
     8461 aaatcaatta aatctaatga ttcttttaaa ttgtcaaaac tcaccgtttg atcaaattca
     8521 aatggcttac cttgatattt ccttaattgc gtaattgacc atttcatatg gcttcacctc
     8581 taacaagcac aaaatttatt ttaactttct aagaacaagt tgtcaagatt ttttcttaac
     8641 atatcatgtt ctgttacaat attaataatg aataccgaaa ggacgaattg caaatgaaaa
     8701 gcgttggctt aatcacagaa tataatccct ttcataatgg gcatcaatat catattaatc
     8761 aatctaaaaa acttacaaat gctgacgtta ctattgcaat aatgagtggt aactttgtca
     8821 tgcgtggcga accagcaatc tataataagt ttactcgtgc aaaaatggca ttatcaacag
     8881 ctgatttagt tatagaacta ccagcaactg ccagtttatc atctggcgat cattttgccg
     8941 aactagcagt taaagtcgca gattatatga gtgtcgatac aattgcattt ggtagtgaaa
     9001 ataatgatat caaaacatta aaacaattag ctcacagcat taatgaaatt gaacaatccg
     9061 aatccttttc acaaaaagta aaagaaggta aaagctaccc tcgtatcatt agcgaattat
     9121 tagagcatca tgaagcacta gcaagtccta acaacatact tggtattagt tatctgaaag
     9181 caattgctaa acatgctaaa aacatcaatg caatttctat taaacgagaa aatgctcaac
     9241 atcatgattc attaattcaa caccatcagt atgcaagtgg tacatctatt agaacatcaa
     9301 tcattagtca agatgatcat tggcatcatg tagtacctaa agatattcaa cacctgtacg
     9361 taacacctca tataacatta aatcaaatat tcccatattt gaaatatcaa atcatagcta
     9421 tgactacaga gtcattaaag aatatttata ctgttactga aggttttgaa aatcgtttaa
     9481 aatctaacat taatgaagca actgattttc atcattttgt aaaatcacta aaaacaaagc
     9541 gttatacata tacgcatatt caaagactgt taatgaatat attattaaac attaaaccta
     9601 cagatgttac gagaaacatt cacgcagtta aagtattggc gatgaacgac cgtggcagac
     9661 aatatttaaa gcatttaaaa acagcgttcc cagaaagaca atatataacc aatattaaca
     9721 aaagtaatgc acattacttc acaaatgaaa tcaaagcaac acacatatac aatgcaataa
     9781 gcgggcaaca acaaacagat ttcaacacgc ctgtcataca gcaatattgt taacaaatga
     9841 aaacagtatt ttaggattgt aaaccatgat aaacctaaaa tactgttatt tttattactt
     9901 aaatttcttc ttcaatgcct tttcaacata aggtggaacg aattcagaaa tatctgctcg
     9961 ataagctgca acttctttaa caatacttga acttataaat gaataattag tactagacat
    10021 catatataac gtttcaattt cattgttcaa ctttttattc attgaagtta agcgtaattc
    10081 atattcaaaa tcactgactg ctcttaaacc acgtatgatt gttttagctc ctacttgttc
    10141 acaataatcg actagtaaac cactaaattg atgaaccttg acattaggta aatgtttaac
    10201 agattgttca attaaatcca tacgctcttc taaactaaac gtaccttctt ttttactatt
    10261 tttaagaaca cagacatgaa tttcatcaaa tctatctgta cttctctcaa ttatgtctaa
    10321 atgaccataa gtaatggggt caaaactacc cggaatgacc gctattgtat gttccatgct
    10381 attctccctt ttctaataac aatgtgtctg tcaacccata atggtaacgt ttaatcatat
    10441 taaacggttg atagtctatt tcttcatgat tgctaaattc acaaacgatg ataccatttt
    10501 ctttcaataa attaaactct gaaattagtt ttaaagcttt atcaatgaga cctttattat
    10561 atggcggatc taagaaaatg acatcaaatt gaatatcacg ttttgacaat gcttttaaag
    10621 ctctatctgc attattttta taaacttcag attgtgcctc taaattcaaa ttcgcaagat
    10681 ttgatttaat aacttttaca gctttaaaat tttgatcaac aaagataacc ttatccatac
    10741 ctcgagagag tgcttctatt ccaagcgccc cgcttcctgc aaataaatct aaacctatac
    10801 ctgacacatc atataaacta ttaaagatac cttctttaac tttatccata gttggtctcg
    10861 tattacggcc ttccatactt tctaaagctt tacttttatg tttacctgca atgacgcgca
    10921 tgttgttcac acttccaatt catttagtta tttaatataa tttattgaga aaaaggagaa
    10981 tgataaacca atgaaacaaa catttattac acttggtgaa ggtctaacag atttgttcga
    11041 attcatgacg atgattgaat ataaccatca acgtattgat aaaattatct attttcattc
    11101 accacaagct gaaaataaaa agtcatctgt agcaatcatt atgaacccta caactggcaa
    11161 tcatttccaa gcattttata tcatgattaa cgctattaaa tatccatatc cagattcaaa
    11221 taaaaagttt caaatgatta atgattgtgc tgaaaaattc gacataccta ttttaggtat
    11281 cgatgtacag cctcctcaag catttcatga tttatcgtta tattataatt atttaattag
    11341 tgtgttaagg ctccaaaaat ggataccaga acttcaataa tattaattat atatttcgtg
    11401 tttctctttt tcgtaagttt tctttaagta tttatatggt gagccatcaa tgtgtctaac
    11461 gtatttaagt tgcattagtt tatgtacaat ttgatcaaca tcttgctcat tcacatacat
    11521 aattacaaat ttacgatctc tatttgaatg aacgatatgt ccatattttc ggatttgtcg
    11581 ttcatgtttc atatgtttta aataaactac aatactagtt cttgggatta aattcattta
    11641 aacctcactc catttatttt cagcattaat catatcatgt ctttattaat tttaaaatat
    11701 taagctacct taattatgtc ttatgtaatt acacgataaa tgtatttaga ttatattaag
    11761 catcattata ttaattcaat tctcaagagc aacactatat gattaaccac acattttgct
    11821 gtagtcagaa ctatgaattt gttaagctat aaaagtaaac gaggaggaat tttttatgaa
    11881 aaaattaaac agaaacattt taaatgcagt actagtcggt gctggatttg taagtagttt
    11941 gttaatgata actaaaaata aagtgattac taaaaagcaa accatccctg attttttcaa
    12001 aggcaatgca ccatatatat ttgcacatcg cggtggtatg actttaagac cagagcaaac
    12061 gcaacttgca tttgactatg ctaaacaatt aggcgttgat ggctttgaaa cagatgtgag
    12121 actcacaaaa gatcaacagc ttatcgtttt tcatgacgca actgttgata gaacgacgaa
    12181 cggctctgga aaagttagtg cccatacatt agcagaatta aaaaaattag acgcaggtta
    12241 tcacttcaaa gatatcaatg gactaacacc atatcgtggt catgcacaca cagctatact
    12301 aaccttcgat gagttgctga agcaatatcc tgatatgtat ataaacgtag acttaaaaga
    12361 tgctcctgaa tcctatgaag gctcaatcgc accacaaatt atgtttgata caattgcaga
    12421 gaatcaagca tttgaccgtg ttcttgtgac aagtttttat aaagaacaaa tcgtacgttt
    12481 taataaaatt gcacaaggat ctgttgcaat tggtgctagc caacaggaag ttacagaggc
    12541 atttttaaaa tatcatttat taggtggtag gtactaccaa ccattagcac aaacgttcca
    12601 aatgcctact cattttaaag gtattaattt gacttcatct cgttttatca aatggttaaa
    12661 tgatatgaat atcattccag gctattatgg cgtaaatagt ataaatctga tgaatgattt
    12721 ataccaaaaa ggtgcacata cgattgtgac agatcgacca gatttagcac aacaattcaa
    12781 acaaacaata aagaacaaac aataaacaaa aacatcgagt cagatgctag ttagtggttc
    12841 caataaccaa ctttttctga ctcgatgttt tttataatga acaattacag gatccgcccg
    12901 tcgcacaacc actatgcgat gtttgaaaga atggattacc tgtttctatt ttgacatgtt
    12961 ctgaaacagc gtacgcaatt ttcgtaacaa cttcatcaat taaatgttga agcgccattt
    13021 ctctagcttt gtaatgcatc acaacgtcaa gcatctcata tgctcttttg cgttgtcttg
    13081 tctctaacat gactttctta taatcaggat gatatttccc gaaacgcatt acttcatcat
    13141 atttctcttt tgattttaaa aatgattgat aaagtaaatg ggcttcgtca ttgttttcga
    13201 gcgcctgttt agcttgctca aatgatgcat aaatatctga tgcaactatc atatcagcta
    13261 gttcatcaat atcatctaat atcgctagtg aagcttcatt aatcataaat acccctcatt
    13321 gttatttttc taagaaaatt agtgaataat aatctctcat tacatctcca cccatctcat
    13381 cgtattttga atttaatagg cgagatcgat gtatatctga attcatccaa ctatggatta
    13441 atgttggtac atcattaaac gcataaccaa cattttgagc agttgtttta taagtaacgt
    13501 gatttttatc taattgccct cttaatgcgt cctctgtaaa ttcaacacta tcagaaccat
    13561 tagaggtcgc ttcatataag ttattagatg caatatgtgc taaatcgcta ttgattttca
    13621 atggttttaa tccttttaat tttctcattt cattcgttac ttcataaaga gaaattaatt
    13681 gatttggatt ttgctcaact ggacgcttat tatgctcttc tgacgtagaa ttagaattta
    13741 atcgataagg ttcaatatct gctaacattt cttttgttaa aaatcgtaca cttagtacct
    13801 ttttcgattg ttgatcagaa tacacttgtg catatatgtt gccatattta atcagtgttt
    13861 gtgtttttaa atcttcatct gaaagttcaa attcatattt tttaccatca actttaaagg
    13921 acggttctgg attaatactt gtatgattaa aaatttctgc agaatgttgt cctattttta
    13981 acggactaac atttactttt tcacctgtag catacactga aacgatttct tcacgtttag
    14041 ttgaaacaat gtaataactg tttttgtctt taaacacata atttttgtaa ccatctctaa
    14101 aaggatagac acgatctgct tgtccaaatt tagaagtaag cactttaata tctttaccta
    14161 cccatgtacc aacaccttct ttaggctttg gattttcagc atggttattt gatttattca
    14221 cctgttcaga tctatttact ttattacctt tattcgggtt ctctaataca tcaaatttta
    14281 atctcggtga ataaaaaaga tatattaaaa atccaactaa aaataataca ccgacgactc
    14341 ttataattaa ctttttcatc aatcaaccac ctaaaaagta ttaatactat tgtaaaaaac
    14401 aacacattaa ttagcaaatt ttcaacactg acataactgt gtcatttcga taaaacaaaa
    14461 tcatcttcag gcataatgtt aacatcgtaa agattttccc taccctctat atcataacct
    14521 atctttatgt tttcaggttg aatttcatta ttaggattaa aatatgtata aaaaaacgga
    14581 cagttaaggt atcaaatgaa taccatcaac tgtccgacta ttcttcatca aaaaacctga
    14641 taaaacaaat tgccttatca gattagtatc atttgtataa gcgtattaat gaccaaatgt
    14701 tgctttaatc agtgatgtcg tttctccacc tggatataat acgtataata ataagtaaac
    14761 tgctacacct gtaattgcag tacagaacca tataactgaa gcgaatggcc cgaatttgcg
    14821 gtgtacatta tatttatctt taaatgctgt aataatttga actagaccca gaataccacc
    14881 aattgttgct aaattaatgt ggaaaaataa gaaaatcgtg taatatttct taattgatgc
    14941 tggtccgcca aatgctgtat taccgataaa tatcgttcta cttgcataga ttaagaaaaa
    15001 agttaaagca aaaacagctg cagctaacat aacatttttg tgtttattta tttccctttt
    15061 ccaaataagt ctccaaccaa tggcaattaa aattgcacta atgacaatac atgtcgtact
    15121 aatcgttggt aaaattggaa cgcccatatt tttcatccta acttaattaa tctagatcaa
    15181 agtaagtaat gaaacaatca cagctaacac gaaaaagatc actaaataat ttagtgaata
    15241 tataaacatt tgtgttgccc attttgtttg atctgaattt ttcttaaatg ttgttaaacc
    15301 taatgcaatc catcctaaat ttaataatgt agctaacact acgaatacga cacctaaatt
    15361 tattagtaat aaaggtactg gcaataaaat aatcaaccag ataaacatac tgacacgtgt
    15421 acgtttaaag cccttaactg atggtaacat tggaatattt gcaagtgcat attcatcttt
    15481 acgtttaata gctaaggcat aaaaatgaat tggttgccaa caaaatacaa ctaaaaacag
    15541 cgcaatcgct gttaaactaa tttgtccttc aattgcaacc catccaatta gtggtggtac
    15601 tgctccagga aaactcccaa tcactgtgtt ccatgttgta tgtcttttag accatattga
    15661 gtaataagac acataaccta caatccccat aagaccaagt acgcctgatg gtatattcaa
    15721 taaaaacaaa caaatttctc caactaacat cataccaaaa cttaatagta ataaattttg
    15781 atctgtaatt ctattgttta cagttggtct attttgttta ctaggcataa tacgatcaat
    15841 atcttggtcg taataattat ttaacgcaca tgcgccaccc ataattaaag tagatcctaa
    15901 tagcattaat aaaatttgag gtattgatga taagaaggaa tgatttgtca ttacaactgc
    15961 tagccatgcg cccgcaaaag ccggaattaa gttaccttga acaagtccca ttttaattat
    16021 ctgttgcaat tctttgaagt taactctgct aatattttgt gacaaagtat gctctttgct
    16081 cataatcccc ctccttaaat ttgttcatat aagattatga tatcttagat tgcataaaaa
    16141 gactaggttt aataaaatta aattgtgaca aattaacgac aagagaaaat gtcaattttg
    16201 tgacacaaat aacatttaat ttattgctat aatgtatatg ttagaaaatt ttaataagta
    16261 gaaacatgca tctaaaagag attaatattt aagcttcaaa tttgagtaac tgtgactgaa
    16321 attttatagc agtgaacggc acatcattgt cgtgttattc atatcattga tgatagtcga
    16381 tcacttctaa tactagaaac aattaaatta tataaactgt gttggatccc atctcttcaa
    16441 atccaactgt taggttcatt catagtatta actcaagttc agctattaaa tcaaaatctc
    16501 agtgatgcgt ttttttatga tggggtgtat aaattgtttg gcaaaaagaa tttaaaatgg
    16561 ttaggtgtcg tagcaacgtt aatgatgaca tttgtacaac ttggtggagc cttagttacc
    16621 aaaaccggat cagctgatgg ttgtggttct tcttggccac tatgtcatgg tgcgttgatt
    16681 ccagaattct ttcctattga tacgattatt gaattaagtc atagagccgt ttcagctttg
    16741 tctttattaa tggtcttatg gttagttatc actgcatgga aacatatagg ctatattaaa
    16801 gaaattaaac ctttatcaat cattagtgtt ggattcttat tattgcaagc attaatcgga
    16861 gctgctgctg ttatttggca acaaaacgat tacgttttag cattgcactt tggtatatca
    16921 ttaatcagtt tctcatctgt atttttaata acattgatta ttttctctat agatcaaaaa
    16981 tatgaagctg acgaattata tatcaaaaag ccattaagac gtttaacatg gttaatggca
    17041 atcatcattt attgtggtgt ttacactggt gcactagtgc gacatgcgga tgcaagttta
    17101 gcatatggtg gttggccatt gccattccac gatcttgtac cacattcaga acaagattgg
    17161 gtccaactca cgcatcgtat catggctttt atagtgttta cgattattat gattacttat
    17221 attcacgctg ttaaaaatta tccaaataac cgtacggttc attatggtta tacagctgca
    17281 tttatacttg ttatattaca agttatcaca ggtgcattgt ctattatgac aaatgttaac
    17341 ttgataatcg cactattcca tgcattattt atcacttatt tatttggtat gacaacatac
    17401 tttatcatgc taatgttacg atcagtaaga agtgacaagc aataacaaaa aagcaaaccg
    17461 taattttaat ggcacgccca ttaaaattac ggttttttta tatcaatatt taaaattaaa
    17521 cctaagccta agacaagtaa aaacgagatt acacgtcaat cgttgtgtaa tctcgtttta
    17581 ttttaatcat tttagtcaga tgctttttca atttcgatta ataaatcgcc tgtcgctatt
    17641 gtgtcaccat tatttacagt tacttgttta atcataccgt caaatggtga ttgaattgtt
    17701 gtttccattt tcatagcttc agtaattagc aacggctgat tagctttcac agtttcacct
    17761 acactaacct tgacttcagt tactgaacct ggcatttgcg caccaatatg acttggatta
    17821 ctcttatctg cttttggctt aacgttcgca tttgtatgca cattttcatc tttaatataa
    17881 atacgtctcg cttgaccatt catcgcataa taaatcgttc tattaccatt ttcatctggt
    17941 tcactaatcg tttctagttt aataattaat cgtttacctt tatcgatttc aatttctact
    18001 gtttcaccat tacgcattcc aaagaagaat gtaggcgtat caagtaacga taagtttccg
    18061 tattgatttc tagtttgaat atattgttca tatacttttg gatataatac ataactaata
    18121 atatcttgct ccgtaacagg accttgttgc tcttcttcaa gcaactcacg gactttttca
    18181 aaatcaactg gctctagata ttcacctgga cgagctgtta atgcttcttg gccttttaaa
    18241 ataaccgctt gtaaatcttt attaaaacca tttacaggtt gtcctatttc acctttgaag
    18301 aacgacacta ctgattctgg gaaatctaat ttataaccat ctgtaatcac ggattgttca
    18361 tcaagatcat tttgtaccat ataaagtgcc atatcaccaa ctactttaga cgatggcgtt
    18421 acttttacga tatcaccaaa taagaagttc actctgcgat acatatcttt gacttcatca
    18481 aatctttcgc ctaaacctaa acttttagct tgttgactta aattcgaata ctgtccacca
    18541 ggcatttcat gttgataaat ttcagtattc ggtgatttga tatcactttc aaagtctgaa
    18601 taataagtac gtacagttga ccaataatga ctaagtgact ccataccttc aatatcagtt
    18661 ctaaggtgac gtgggaagcc atttaatgca taatataacg aattggcgct tggctgactt
    18721 gttaaaccac tcattgaagc aacagcagta tcaatgatat cgacaccagc atctattgct
    18781 tgtttgtacg ttaataaacc attaccactt gtatcatgag tgtgaagatg gattggtaaa
    18841 tctacagctg cttttaactc accaatcaat tcgtaagcgg ctttaggttt taataagcct
    18901 gccatatctt ttatcgctaa aatatggaaa ccttcacgtt ctaactcttt agctagtttg
    18961 acataatact ctaaagtata aatgtttgat cgttcaggat ttaaaatgtc acctgtataa
    19021 caaatagtac cttctgagat tttgcccgct tcttgtactg cttcattggc aactttcatt
    19081 tgatctaccc agtttaatga atcgaaaatt ctaaagacat ctatgcctgc tttagcactt
    19141 tcttgtacga atttatgaat aacattatca ggatagtttt tataaccaac tgcgtttgaa
    19201 gcacgtaaca acatttggaa taatacattt ggaatagctg tacgtagacg ttcaagtcgt
    19261 tcccatgggt tttccttcaa gaaattatat gccacatcaa atgtagcacc gccccacatt
    19321 tctagtgaga aaccatcttt aaatacgtcc gctgttttgg atgcgatatt aatcatatcc
    19381 ttagttctaa ctcgtgtagc taacaatgat tggtgtgcat ctctaaaagt tgtatctgtt
    19441 aataagacat catcctgctt tttaacccat tcagctacac cttttggacc tacttcatca
    19501 agtaattgtt ttgtaccact aaatgaagcg attttacttg aagatacagt tggaattgat
    19561 gctaattcat agtctggttt cggacgtttc tcaacatttg ggaaaccatt aattgttaca
    19621 ttacctatat attctaatgt tttagtacct ctatctagag acggctgaat gtcgaaaagt
    19681 tctggtgttt cttcaataaa tttagttgtg taatcaccac ttgtgaactt tttattcttc
    19741 attacattaa ttaagaatgg aatattagtt ttaacaccac gaatacgcat ttctcgtaat
    19801 gaacgtacca ttttttcttc tgcttgttta aatgatatcg cgtgtgtaga taattttacg
    19861 agtaatgaat cataataagg tgatatctca gcaccctgga aaccatctcc agcatcaaga
    19921 cgtacaccaa agccaccgct tgaacgataa gcaatgattg ttccagtatc cggcatgaaa
    19981 tcatttaacg gatcttctgt tgtaatacga cattggatgg catagcccaa tgttgtaata
    20041 tctttttgtt gtggcatatt aatttcttca ccaaataaat cggcacctgc tgcaactaaa
    20101 atttgtgtct taacaatatc aattcctgtt accatctctg taattgtatg ctctacttgt
    20161 acacgagggt taacttctat aaagaagaat tcgtcaccag atactagaaa ttcaacggta
    20221 cctgcattga catatttaat attttccatc aattgaattg cagcatcaca aatacgttga
    20281 cgtagatctg atgataagcc tacagatgga gcaacctcaa caactttttg atgacgacgt
    20341 tgtactgaac aatcacgttc aaataggtgt acgatatttc catgttcgtc acctatgact
    20401 tgtacttcaa tatgctttgg attatcaatg tatctttcta tgtaaacttc actattacca
    20461 aatgattttt ctgcttctga ttttgctcta tggaaagcat cttctaattc actttcttca
    20521 cgaacaattc tcataccttt accgccgcca ccacttgtgg ctttaatcat tagcgggaaa
    20581 ccagcttctt ctgcaaattc ttttgctaat tcatatgatt taattggacc gtctgtacca
    20641 ggaataactg gtaaatctgc cttgatagcc gttgtacgag ctttaacttt atctccaaac
    20701 atatctaaat gttctaaatg aggaccaata aatttaatcc cttcttctgc acaacgacgc
    20761 gcaaattgtt cattttcact taaaaatcca tagccaggat gaatcgcatc cacattcgct
    20821 tgttttgcta catcaatgat acgctcaata tttaaataac tttcagcagg acctaaatca
    20881 cttccaacta aataggattc atctgcttta tatctatgta atgaactttt gtcttcattc
    20941 gaataaattg caactgtgct gatgtctaat tctgctgccg ctctgaatat acgaattgca
    21001 atttctccac ggttagcaac aagtaacttt tttatttgtt tcaatagcga tacactcctc
    21061 aaactattag aattttctaa ctaattagat aataaaattt tatcttaaag cgctctgttt
    21121 tgctatagtt atgtttcaaa ttttcaaaat ttaacattct tgagacaatt aaaacctccg
    21181 cttcaaaaat aataatttca aaaatgatta tgcaacaaca ggtagttcac gtttttgttg
    21241 tgaaacattt tcgatttcta caactctaaa aaattaaaaa taaaattgta aaacatcaac
    21301 atttattatc aatagcgata actttatctt atcatcatga ttctaatttc gccaccacat
    21361 ttagtgattt ttagtcataa aatttagtta taattatacg ttgttttgtt tataaaattt
    21421 gataatcatg agtaatattg taatatcaaa acaaaaagga agttaagcct tgtttggttg
    21481 cctaacttcc gttattgaac tcatcagtcc aatttgacaa agagtatata aatagccaat
    21541 gtttagcatt gcttaatgac agaagtcatc tcgcgctaaa tattggctag tattttttaa
    21601 ttaaattgtc ttcttatatc aactttttgt tgttgtttct ttcgttgctg gtctactttg
    21661 atttgtttac ctacaatcag aagtaatccc atagcaatac ttaaactaat cattgatgat
    21721 ccaccaaagc tgataaatgg caatggcaca ccagttaatg gaattgttgc cgaaataccg
    21781 ccaatgttta caaacgtttg gcttccaaag tatgtggcaa tcccaacaca cacaagttta
    21841 taaaaatatg atgatgtttt atttgcaaac tggaaggcac gatatacaat aaagaactct
    21901 aaagtaataa ctagcaatcc tccgattaaa cctaattctt cgcaaataat tgcaaaaata
    21961 aaatctgtat gtggttctgg taaatagccc aatttcattg cactatttcc taatcctttt
    22021 ccaaatacgc caccgttacc tatcgcaagc aatgaattgg aaatatggta tccagttcct
    22081 gattcgaatt ggaatggatc tgttagcgta ctaaatctgg cagttaaata acttggtaac
    22141 caaccagcca ttaatgcaat gacaaatact actaagaatc ctagtactgc tggtattcca
    22201 aatcttagga ctttgtttac cccaattcct gaataaaaaa taatcgcaac taaaataatt
    22261 aatattagta atgtttgccc tacgtctttt tgtaagaaaa ctaaaaacgt acaacctaat
    22321 gctaatacaa taggacttaa aattaatttt ggtttactta atactctagg cattttttta
    22381 ctaatcataa atgggatata taatatgatt gcaattttta ataactcaga tgcctgtaag
    22441 ttcataaatc ctaagtttat ccaactttta gaaccattaa tatctttacc gattactaac
    22501 gtcagtaata atagcgaaac gatagttata atcatacctt tttgcacttt aatattactc
    22561 agtaatttaa cattcattaa aaatgcaata aaaaatacaa ttataaaact cattatgaca
    22621 tatgctaatt gtcggttgta aaaatacgtt cctggaacat cgataccacc agtcaatgta
    22681 cctttagttg ctggaaccat actggcacta tataccatca ctaatccaat taaactcaat
    22741 acaatatatg taactaataa cgggtaatca ataaactttg aggttttacc aatataccgt
    22801 aaaatacttc taaaattctt catatattca gtcatccaat tctacaattt atacttcaaa
    22861 tgatttcgtt ttatacttta attttaaaaa tccatgttac ttattatata catgacacag
    22921 tcataatcac aaatattaac aggtatcttg aggtaattta tagaatattt gtttacttaa
    22981 tatcgtgaat tgctaaaacc caataaaagt gactgacatg tcatcaagaa gacaacataa
    23041 cagccacttt aatcagtaat taaattataa atataaaatc ggcgctgctc aattgtcgat
    23101 atctcacgcc gttttaatat gcttatatat taaaacttaa acaagtgtaa aagcttcatg
    23161 taattttgaa agttctttct caagacgtaa cataatttgt ttgccatctt cgcggtcaac
    23221 taaacctaat ttaacagcaa aatcaacttc tttttgaagt ccaaacattt gtgtatctag
    23281 tacttcttca tataatgggc atgaaggtaa tgttaaatta tctagttgaa ctttaatcag
    23341 atgcaagatt tcatcagcat ctttagtcaa ttgtttcaaa gctgcatttt tcattgttgc
    23401 ttgtttcgcc atgacacgct ccccctctat ataaattctc aagtcttact aaaagtttat
    23461 cttactaaat attaaaaagc aagtagattt aaaatgtgtc gttagcaatt tgtgttaaaa
    23521 taatgctata tacattgata gggagtggga aaatgataca aattaaagga gctgtaaagt
    23581 ttcctataac attagatagt acgacttgga ttttcgatga tagaaaagtt agcatcgaag
    23641 atttagaaaa aggtgtattt gatggttcaa aaccaatcga ctttgaagat aatcatgaat
    23701 ggaatcgtgc tatcctagaa ggacaaacta atcctcctac tttaaattca gaaataaaat
    23761 ataaaaaacg tgccatgtta gaaggttcat ttgtcattaa tatgacacca tttttcaaac
    23821 attccgaacc acatgactct gcaactacta ttcgattatc aaatgagcat gatcatttag
    23881 atataccgat agatctattg ccctatttat ttttccaatt tgcaaaagac ggtaaacgac
    23941 tttacagcga taatggtgta gatagtttta tttacaatca agaagatggt tattcatatc
    24001 gaatcaacca tgtaactcat attgaggtga tttaatttga gacaagtgca atgcattatc
    24061 tgtgatgcaa aagtatttat agatgagcgc acaactgagt ctaaacgact taaaaacaat
    24121 cctattcgaa catttatgtg cgatgattgt aaaagtcgac tagacacccc taaacaacgt
    24181 gcgcaacact atccgctcga ttaatatctg aattaacaag ttagaaattt gaaaatgtga
    24241 aagtaacagg aactgatagc attaaaacac tttttcaaat attatatgcc aaaagagaag
    24301 ctaagcaact ttcatcgctc agcttctctt ttattatgtt aattattcat tttcttgcat
    24361 catttgtttg atacgctttg cttctttttc acgaacattt ttgtttaaaa ttttctttct
    24421 taaacgtatg ctttctggtg taacctcaac aagttcatca tcattaatga attgtaacgc
    24481 ttcttccaat gttagaatac gcggtctatt cattgtttgt gtttggtctt tcgttgcaga
    24541 acgtacgtta gtttgatgtt ttgttttagt gatgttaaca gttaaatcat tttcacgatt
    24601 atgttcacca acaatcatac cttcataaac ttcagtacca ggttccatga agtttacacc
    24661 tctatcttca agtcctaaaa tggcataagt acttgcagaa ccttgatcca ttgaaattaa
    24721 tgcaccatta cgacgaccgc caatttgtgc tttaatacgt ggtctaaatt cttcaaatgt
    24781 atggttaata ataccgtaac ctcttgtcat tgacataaat tcagtcgtat aaccaatcat
    24841 accacgagcc ggtacattaa agattaaacg tgtaagtcca ttatcagttg tagtcatatc
    24901 aaccatttca cctttacgtg cacctaatga ttcaataacc gcacctgcat tttcttgtgg
    24961 cacttcacat tgcacacgtt caaatggttc acacattaca ccatcaattt ctttaataat
    25021 tacttgtggt tttgaaactt gtaattcata accttcacga cgcatatttt caataaggat
    25081 tgataaatgc aattcacctc gaccagcaac tacccatgta tctggagaat ctgtgttaga
    25141 aactttcaaa gatacatctg tttctaattg ttgatttaaa cgttcttgaa tttgacgcgc
    25201 tgttacaaag tcaccttcac ggccagcaaa tggtgaattg ttaactttaa atgtcatttc
    25261 aagagtaggc tcatcaatac gtagaactgg caatgcttct tgatggtcat gtggtgttac
    25321 agtttcacca acgttaatgt cttccatacc tgaaacagca attaaatctc cagcctgcgc
    25381 ttcttcaatt tctaaacgtt ttaatccaaa gtaaccaaag attttagtta cacggaagtt
    25441 tttcactgta ccgtctaatt taattagtga tacattatct ccgacacgca ttttacctct
    25501 gaatacacga ccaataccga tacgtccaac ataatcattg tagtccaaca atgctacttg
    25561 gaattgtaat ggctcatcac tgttatcaat tggagctggt acataatcaa taattgtttc
    25621 atataatgat tgtaaattat catcttgttt ttcaggatct aagctagctg taccatttac
    25681 tgctgaagca taaacaacag ggaattctaa ttgttcatcg tttgcttcta attcaataaa
    25741 taaatctaaa acttcatcta caacaccctc tggacgtgct gatggtttat caattttatt
    25801 aacaacaaca acaggtttca ggttttgttc tagcgctttt ttaagtacaa aacgtgtttg
    25861 aggcattgta ccttcatacg catctactac taagacaacc ccatcaacca ttttcataat
    25921 acgttctact tctccaccaa agtctgcatg tcctggtgta tccaaaatat taatacgtgt
    25981 acctttataa tcaacagccg tatttttggc tagaatcgta attccacgct ctctttcgat
    26041 atcgttagag tccattgcac gttcatcgac atgttcattt tctctgaata taccagattg
    26101 ttttaacaac tcatctacta aagttgtttt accatggtca acgtgagcaa taattgctat
    26161 attgcggaca tcttctcttt tattagtcat ataaatagtt tccttctttc ttaagtaaaa
    26221 tcccactttt aattacaact tttttattat atcatataat tgagaaaaga taagcaaaag
    26281 gtggggatag gatgaaacaa aaaaaatcta aaaatatctt ttgggtattt tctatattag
    26341 ctgttatttt cttagtatta tttagttttg ctgttggtgc atcaaatgta ccaatgatga
    26401 ttttaacatt tatattactc gttgcaacct ttggaattgg atttactaca aagaaaaaat
    26461 atcgagaaaa cgattggcta taagtcatat cgacaataac acgcgttgat taaagaccac
    26521 acatgttcat gatgaacatt taaatggttt tatattggcg cgttttttta ttatttaaga
    26581 tataaaaacg taatatgtca cctaattgtt aggactacat attacgcttt tggtaattat
    26641 tttgattttc ttttaaatcg ttgttcatgt aattgtatta acgcatcgcg atgtggctct
    26701 aaataatcat tgctaatttc ttgatggaga ccacgatttc caactaagat tgaatttgga
    26761 ccactaattg ttaatggttc tcctagtaaa tttgaagctt gtccatttac ttcatataaa
    26821 ataaccaatc cgccagcaaa atcccatggt tgaagtcttg gcgtcatata tgcttctaaa
    26881 ttacctgtag caactgaaac gatttcaagc gctgcactac catatgctct tgcacttcta
    26941 gaatcattaa caatttcttt aaagatttct cctaaaattg gtttagttaa ccagttggga
    27001 ttgatcccaa taatgctttg tcttagatta gaatcattca atggtttcaa gggttggcta
    27061 cctcgatatg caccttcccc tacttttgca tgatataaga catcagccat aacatcatat
    27121 acaaaacctg cataaggttt accatcgata taaataccaa ttgaaatcgc gaaattttct
    27181 tgttgatgaa caaaattcaa tgtaccgtct attgggtcaa caacccatac cgtacctttg
    27241 gaagtatcga tgtcatgacc atgcccttct tcacctaata cttgatgatt gggatatgtt
    27301 tctaaaattg tatcaaaaat aaaatcttct gttgctttat ctacatttgt aacaaggtca
    27361 ttcggatttg actttgtttc aattgttaaa ttttgctcca tcaattgttt aattctaatt
    27421 cctgcttctt gaataagtcc ttgggcaaat ccataaagtg ccataacatc atctcctaac
    27481 atctttatta tacatcaaca ttttataaaa ataacttctt aagataaaaa tgaaaatact
    27541 aattttaatg agagcatctt aagctgctat tgataaaaca tatgctacaa tatttttaaa
    27601 cactatacag gaggtactct atgacaaaat atacatttaa acctaaagat ttcaaagcgt
    27661 tcaacgtaga aggcttagac gcacgaatgg aagctttaaa cgaatacata cgaccacaac
    27721 tccatgaatt aggagaatat tttagtgact tctttacaag tcaaactggt gaaacatttt
    27781 atcctcacgt agcaaagcat gctagaagaa gtgtgaatcc tcctaaagat acatgggttg
    27841 cttttgcaac aaacaaaaga ggctataaaa tgttacctca tttccaaatt ggtatgtttg
    27901 aagatcaact gtttgttatg tttggaatca tgcatgaagc aaaagataaa gcaacacgtg
    27961 caaaagtttt tgaaagaaaa tttaaagcta ttcaacaatt acctgatgat tatcgtgttt
    28021 gcttagatca tatgaaacct gataaaccat ttattaaaga tttaacagat gatgatttaa
    28081 aagaagcgat acaaagagcc atcaatgtga aaaaaggtga attctttata gcgcgtgcaa
    28141 tcacaccaca agataaaaga ttaaaaagtg acaaagcatt tattgcattt ttagaagaaa
    28201 ccttcgatca gttcttacca ttttattctg cataaataac tttgtttaaa taatagagca
    28261 cgtaatcaca tccatgattt cgtgcttttt ttcttaccat taaatcgaac gttcaacata
    28321 ataattcata cttttaaaaa attaaaataa atttaggttg acctaaacat tttattaggt
    28381 tattatattg tccataagaa gtagaggtga gtcaaaatga ataataaaag acattcaaca
    28441 aatgaacaat taagtttaga cgaaataaac aatacaatta aattcgatca tcgcagttca
    28501 aataaacaga aatttttatc atttcttgga cctgggttat tagtcgctgt tggttacatg
    28561 gatcccggaa actggataac atcaatgcaa ggtggcgcgc aatatggcta tactttgcta
    28621 ttcgtaattc ttatttcaag tttatcagca atgttacttc aaagcatgac agtgagatta
    28681 ggaatagcaa caggtatgga cttagctcaa atgacacgac attatttatc aagacctatt
    28741 gctataatct tttggatcat tgcagaacta gcaattatcg ctacagatat tgctgaggtt
    28801 attggtagtg ctattgctct taatctccta tttaacatac ctttaatcgt cggtgcacta
    28861 ataactgtac ttgatgtatt tttactgcta tttataatga aatatggttt tagaaaaatt
    28921 gaagctattg ttggtacatt aattttcacg gtgttattca tctttatatt tgaagtctat
    28981 atttcatcac cacagttgaa cgctgtgtta aatggattta taccacatag tgaaatcatt
    29041 acaaataacg gcattctcta tattgcatta ggtattattg gcgctacaat tatgcctcat
    29101 aatttgtact tacattcatc aattgtacaa tctagaacat actcaagaca taacaatgaa
    29161 gaaaaggcgc aagcgattaa atttgctacg atagattcga acattcagtt atcaatcgca
    29221 tttgtagtca attgcttatt attagtgtta ggagcatcac tatttttcaa ctcaaacgct
    29281 gacgatttag gtggtttcta tgatttatat cacgccttaa aaactgaacc tgtactaggt
    29341 gcaacaatgg gtgcaatcat gagtacatta tttgcagttg cattattagc gtcaggtcaa
    29401 aattcaacga ttactggtac tttagcagga caaattgtaa tggaaggatt tttaagatta
    29461 cacataccaa attggttaag acgtttaatt acacgttccc ttgctgtcat tcctgttatc
    29521 gtatgtttaa tcatttttaa aggtaatgca gctaaaattg agcaactact tgtattctct
    29581 caagtatttt taagtattgc tctgccattc tgtttaatac cattacaatt agctactagt
    29641 aataaagact tgatgggacc tttctataat aaaacatggg ttaatatcat ttcatggaca
    29701 cttattatta ttctaagtat tttaaatgtc tatttaatcg ttcaaacatt ccaagaacta
    29761 caaggttaaa aataaaacca ttaaaatgtt ggttgtatct aataattaac aacttaacat
    29821 tttaatggtt attcttttgc tatgaacata ttaatgataa atatactcat cgtccttatg
    29881 tgtagtttct tttgattctt ttttagaaat caacataatc atcacgataa tccataagac
    29941 aattgcaatt aaattaagag ccatcaaact tacaattatc gcgataacaa aaagtataat
    30001 cgataatttt ctacgtcctt taatacaaat tgtcgcaaat ataccataaa gtgttgaaat
    30061 taataaaata attgctaatg cacccattgt tgtctgaagc atatctaaag aaaccttgcc
    30121 accgttctga gataattgct tcgctaattc ttcacgttgt gatgcattac cactttttaa
    30181 agaaaaatag gatagcccca taactaataa ataaataata ctaatactat ttgcaatcca
    30241 agccatgatt aattcaggtt ttctattcat cttaaaatcc tttctttgtt taatatttta
    30301 ataagtatac tacgagccta tagatatacg cacattaatt tacttgtata ttaacatatt
    30361 tttcatctaa tttttataat aaacatctga attcagattg atggtaattt aggtgtatgg
    30421 taatgagaaa tcgtcattta aaattttgac acctaaacaa tttaattaaa cgttagcgat
    30481 aattaacctc atataaaaac ccagattaat aagcaataga cctctactta ctaatctggg
    30541 tatcatcaat atttattctt gctctttttt gtccttaact tcacttgaat tgcgttcttc
    30601 agaaatatca ttgttgtaaa tttgacgtcg ctcttcaaaa ttcattcgac gttgactaac
    30661 tgcgctaggt tgatttttcc tacgttcttt cattcgttgg ttataagctt tacgtttttc
    30721 ttttttctta gctttaattt caactttttc tcgcttttgt tgtgctttca attcttttgg
    30781 atcaacttca ggttctgcat aataaccatc atcttcaata cctagttctt cagtactttt
    30841 ttgtttgtat ttagcctgtc tggataatac tgatggttct tcctcagtgc gctcatcatg
    30901 attatattca ctttcaactg cacgtttagg aaattgatca tatttatctc tttcctgttc
    30961 ttgatcatat acagtgtttg atttatccaa ttctttttca tatttaggca catcataata
    31021 atcatctttc ggttgtgggc gctcatacac aggttcacga taatcataaa tcggcccatt
    31081 gtactcttca taatattgtg gcgcaaccat ttcaacttta tcttttctta taaatagcat
    31141 catcgcaata ataaagaata atatcggaat gattaaagta acgataagta aaactaaagg
    31201 aatcgttaca attgctgata ttaagaataa gaaacctgac aagattctta tattcataga
    31261 tatgagagct aaaaatgaaa tcaacaaaca aacaataaag taaacgataa tagcccatat
    31321 accattttgt aaccatatta caaattgtgt tgtattatag tcatttccag ctaataattg
    31381 ctggataaat gcattattgt ttaatgtatt ttcaagatta gcaatcgatg tgttattact
    31441 gaatgaaaca agtgctataa acatcgtaat gacagtaagc actaataaca tcacccaaca
    31501 taaccaacct agaacttttt cagttaatct acttactgga cgtttaattt gagtaaattg
    31561 ttctccagtc attcgttaca actccttata gtacttatcc cgttattata actaaatata
    31621 cagtaaataa ctactattta tgattttatt ttaatgacat tttgaaattc aaaaagtttt
    31681 cattgtattc acttaaaact tcaggtccta aatctttata aacttcaagg cgttcttgct
    31741 ctttcttagt cggatagaaa cgatggtcgt ctttaacctc tttaggcaac aattgtcgag
    31801 cagccttgtt tggcgttgca tagcctacga attctgtatt ttgcttgtta tttttagctt
    31861 ctaataaaaa attcataaat ttatatgcac cctctttatt ttgtgccgtt tttggaatta
    31921 ccatattgtc gaaccataaa ttcgatcctt ctttaggaat aacatagtta tatttatccc
    31981 cttcttgcac tagaggtgct gcaacaccac tccaaacaac cgctatgtta ccttcatttt
    32041 gttgaagcat catggtaatt tcatcaccta cgacacctct tacttgtggt gctagtttgg
    32101 ttaaatctcg ctctgcttct tttaaatggt gcgaattacg gtcattaaga ttatacccaa
    32161 gtttattcaa actcatgcct ataatctctc tagcaccgtc aactagtaaa atttggtttt
    32221 taaatttagg attatacaac gacttccaac tatcaaatga ttcatttgga tacttttctt
    32281 tattatataa aatacctaca gttccaaaaa aataaggtaa agaatattta ttgcctttat
    32341 caaatgacat attcatataa tctgaatcca aatttttaat attaggtacc ttattatgat
    32401 ctattggtaa caataaatga tctcttttca atttttgaac tgtatattca ctaggaaaag
    32461 caacatcata atgtgtaccg ccattgcgaa ttttggcttc catcgcttca tttgaatcga
    32521 aagtttcata aacgacttga atgccagttt ctttttcaaa tttcttaatt aactctgaat
    32581 caatatattc gccccaattg tatacgtaaa ttttttgatt tgtatgcact tgttctttag
    32641 atttaaacca atgacttaaa gtaagacaaa gcatacccac aactaatgca cctataatga
    32701 gttgtaaaaa acgtttcatt attttacacc tcgcttgatt agttttttct tatttatcac
    32761 gtattgaatc aaataatatc ctagtattcc taatacaata acagcaaaca ataatgttga
    32821 aatcgcatta atttccatac taattccttt tctcgccata gcataaactt caactgataa
    32881 cacactaaag ccattaccag taacgaagaa acttactgtg aaatcgtcta gtgaataagt
    32941 taaagccata aagaaccctc ctataataga aggtaaaata ttaggaataa taatgttgct
    33001 taataattgt ggttcagtcg ctcctaaatc tcttgcagca tttaacatat tattattcat
    33061 ttcatacagt tgtggtaaga cgataatcac aactataggt atgcaaaatg caatatgaga
    33121 tattagaact gtccaaaatc ctaaaccaag accagtaaaa tggccaatcg ttgtaaacat
    33181 aattaagaat gatgcaccaa tgacaacgtc ggatgatacc atcaagacat tattcaatgt
    33241 tagtaaagtt actttaaact ttttatttct taaataataa atagcaatgg caccaaatgt
    33301 accaataact gtagaaattg aagcagctaa aagtgctaca gctatcgtat taaaaatgac
    33361 cgacattaaa tgatcatttt gaaataatga ttggtaatgc tctagtgtaa aatgttcaaa
    33421 gtgaatcata ttaccagccg aattgaaaga atagaacatt aaaaagaata ttgggatgta
    33481 taaaatcgct aaaagtatcc cgatatacag ctttccatac catttcatat gactcaccct
    33541 ctcccattag atgattttgt aatgattaaa atgaatgcca taaatacaat taagaatata
    33601 gctatagttg atcccatacc ataattttga attgttaaaa attgttcctc tattgccgta
    33661 cctatattta tgactttatt acctgcaatt aatcttgtaa tcataaataa tgaaagtgat
    33721 ggaataaagg taacttgaat cccagtcata acaccttctt ttgttaacgg catgattact
    33781 tttctaaaag tatagaatgg actagcacct aaatcacttg aggcctgcaa taaattatta
    33841 ggaattgctt tcatgctatt aaatatcggt aaaatcataa atggtatata aatgtaactt
    33901 gccactacta aaaacgcacc agttgtaaat aacaaattga atgatggtaa attaaataag
    33961 tggaaaaatt gattaatcac accatcgtga cttaataaac ctataaaagc atatgtcttt
    34021 aacaataaat ttatccatgt tggaataatc attatcatta ataagatatt ttgaaatttc
    34081 gagcgagtaa tataataggc agctggataa ctgatagcca acgtaataat tgttattaaa
    34141 gcggcatata aaattgaata tgcaaacatt tttaaatatt ttgtagtaaa aatttgttga
    34201 taattcgtga aactaaaatg tccattgata tctaaaaatg aaaaataaat gagtaataca
    34261 actggtacaa taataaatat aaccatccat aataaatacg ggattaagag aaatttatta
    34321 gtattacgca tggtctactt cctcatagct ttcaatacgt ttatcaaact cttcttctgt
    34381 ttctccagga accataatat gaatggcttc aggatcaaaa taaagaccaa cttcactgcc
    34441 tacttcggct tttttagtcg tttgtattac ccattcataa cctttattgt ctatacaaca
    34501 tatttcatag tggacccctc taaataacat agaatcaaca gttgctttaa ataatccttc
    34561 ttcagctttg attaatgata tatcttctgg tcgaataacg acttctactt ttttattttc
    34621 aggaataccc atatcgacac attcgaaatc ttgcccataa atattcacga cataatctct
    34681 aaccatgcgc ccttcaacaa tattagattc tccaataaaa tcagctacaa atcgattcac
    34741 tggttcgtca tatatatccg ttggtgtacc aaattgttga attttcccat cctttaatac
    34801 aaaaagaaag tcacttagtg ctaacgcttc ttcttgatca tgtgttacaa atataaatgt
    34861 aatacctaat ctagattgca attctcgtaa ttcatattgc atttcagtac gcaatttcaa
    34921 atctaatgcg gataaagatt catctaacaa taatatttct ggttcattta cgatagcacg
    34981 tgcaattgca acacgttgct tttgtccgcc actcatttca ttaatattcc ttttttcata
    35041 acctgaaagt tttactaatt ttaatgcctc agttactttt tgatcaattt cagtttttga
    35101 taattttttt aatttcaaac cgaaagcgat gttatcatag acgtttaagt gtggaaataa
    35161 tgcataatct tgaaagactg tattcacttt acgtttattt ggcggtaaat aaccaattgg
    35221 tttgttttga taaatcactt caccactgtc aggatattca aaccctgcaa ttaattttaa
    35281 aattgttgtt ttaccacaac ctgaaggacc taataatgta taaaagtatc ctgattcaat
    35341 atcaatatct atgtcatcta agatattaag atcatcatag cttttactaa ctgattttaa
    35401 tgataataac ggttccaaat aagatccccc ctataaatat gaagctgtcg ctacaataag
    35461 tattcgtgtt tcattgtttg attcatttga caaacgatga ttatctcgtg ctttaaaata
    35521 caaaacgtct tcttcttgtg cttgatatac ctctttgcct aaattaagtg ttatctgacc
    35581 tgacatacaa taaataaacg tatcagactc tgatggattg aaatttttat atgatgcgcc
    35641 aggctttaaa gttaatatta atggctccat atcatattca tttgactttg aaactaacca
    35701 atttaatata taaccttcat catactcatc ataaataact tgttcttcct tcttgtataa
    35761 tactttttca ttttcactgt ctttaaaaaa ttcacttggc gtcgttccta acacctctat
    35821 aatatttaag aaagtttcca tacttggtga ggcatgttca ctttctattt gtgaaatgta
    35881 gcctttcgat aagtctgtac gttcagcaag ttcttcttgc gttaaatttt taattcttct
    35941 aagattttta attttattac ctatgttcat ttatttttcc acctaaaaaa gctatattaa
    36001 gtttactttt atcaaacatt ttgtttaaca cttgataaag gtatcaaaat atagctttat
    36061 tagcaatagt tttaaagaaa aaatcctatt aaattgtgtt aatttcgaac gaattatttt
    36121 ttagctgtta ctctttgttc ttcagagtga ttttttacat cttgaactgc tttgtaacta
    36181 ttatagccac tacttttttc gaaagtatta aaaatttgtt tttcctctgc tttagcaggc
    36241 acaatttctt taaatctttt atatgcatcc ataaagtcgc ctgccgtcac gccggattca
    36301 taatacttct ctacatgatt aaagaaattt atcactgaaa tcatctcttc attactccag
    36361 tctaaatcaa ttggatactc atattccatt gttgaacgcc tccatttcaa ttcttttgtt
    36421 agcatcgcta gtataacatg catgtcacat gttaacgata tctcaaatca atttccttac
    36481 actactttac ctaaaacaaa aaagccgtag catatgctac agcttttaat gactgaatct
    36541 ttatagataa tcagttatta cattgtatgg attgggtatc cgatagcttt ttccgctgct
    36601 tccatagtca tctcacctaa tgttggatgt gcatggattg ttaatgcgat atcttcagca
    36661 ttcataccag cttcaattgc taaacctaat tcagagataa tatctgatgc accagtacca
    36721 actacttgag caccgattaa agtatcatct tctttaagtg taataagttt aacaaatccg
    36781 ttagtatcat ctaatgataa tgcacgacca tttgctgcat atgggaattt agaagcttta
    36841 attgctaaac cttcttcttt agcttgcgct tctgaataac caactgtagc taattctggt
    36901 tcagtaaagc atactgctgg cataccaatg taatcaactt cagcagcttg accatcaatt
    36961 gcttcagcag caactttagc ttcatagcta gctttgtgag caagtggtaa acctggaacg
    37021 atatcaccaa ttgcatagat attgctgata gacgtacggc tttgtttatc aacttctaat
    37081 aatccacggt cagcgaattt aacacctaat tcttctaggc ctaattcgtc tgtgtttgga
    37141 cgacgaccta cagttactaa tacataatca gcttcgattg ttttctcttc gcctttagct
    37201 tcataagtaa ctttaactcc gttatctgtt tcttcagctg atttagccat agcttcagta
    37261 acgatttcaa cacctttttc tttcatacct tttttaacag gttgtgtcat ttgtttttcg
    37321 aagccaccta agatatcttt agcaccttca aggattgtta cttctgaacc aaagttagca
    37381 aatgctgtac ctaattctga tccaatgtat cctccaccaa ctacaactaa tttacctggt
    37441 acttcttgta agtttaaagc acctgttgag tcgataacac gtttaccgaa tttgaaatta
    37501 ggaatttcaa ttggtcttga acctgttgca atgattgcat ttttaaagtt gtatgtttgt
    37561 gcgctctttt cgtccataac acgtaagcta ttgttatcta cgaaatatgc ttcaccttta
    37621 acgatgttaa ctttgttacc tttaagtaag ccttcaacac caccagttaa tttattaaca
    37681 actgatgatt tgaattcttg aactttttgg aagtttaaag aaacactttc agcaataaca
    37741 cctaagtttt cagaatgttg tgcttcaaca aaacggtgag aagcatgtag taaagctttt
    37801 gaaggaatac atcctacgtt taagcaaaca ccaccaagat tacctttctc aacgattgtt
    37861 actttttgtc ctaattgagc tgcacgaatt gctgcaacgt atccaccagg acctgctccg
    37921 attactatag tatctgtttc aattgggaaa tctccaacta ccatgtttta cccctccatt
    37981 aataataatt ctggattatt taataaacgt ttaatgtgat tcattgcatt ttggccagtt
    38041 gcaccatcaa tttgtctgtg gtcaaagctt aatgataatg ctaatactgg tgctgcaaca
    38101 atttctccat ctttaacgat aggtttttga gcaatacggc caattcctaa gattgctact
    38161 tctgggtgat tgataactgg agtgaaccat tgtccaccag ctgaaccgat attactgatt
    38221 gtgcatgttg cacctttcat ttcatcggct gttaatttac catcacgtgc tttaacagct
    38281 aattcgttaa tttcatcaga aatttggaaa atagatttac ggtcagcatg tttcactaca
    38341 ggtactaata aacctctatc agtgtctgct gcaataccga tattccagta atgtttatgc
    38401 acaatttcac cagcttcttc attgaatgaa gtattaagtg ctgggtattt tttcaatgca
    38461 gaaacaagtg ctttaacaac atatggtaag aatgttaact tagtaccttg ttcagctgcg
    38521 atttctttaa atttcttacg gtgatcccat aatgcttgaa catcaatttc atccattaat
    38581 gttacatgag gtgcagtatg cttagagtta accattgctt tcgcaattgc tctacgcata
    38641 gcagggattt tttcagttgt ttctgggaag tcgccttcta atgatactgc tgcaggtgct
    38701 gcaggagttt cagcaacttc ttcatttgaa gctgaagcag ctgattcatt tgaagctgtt
    38761 ggtgcaccac catttaagta tgcatctaca tcttcttttg taatacgacc atttttacca
    38821 gaccctgaaa ctgctttaat gttaacacct ttttcacgtg cgtatttacg tactgaaggc
    38881 attgctttaa cagttctgtt ttcatctact tcttcagttt gagtagctac aggtgcttgc
    38941 tctgctggcg cttcctcttt cgcaggttct tctttagatg atgaatcatc atcatgacct
    39001 ttaaattgca tatcttctgc atcaggtgca tcgattttaa caataacgtc accaactaca
    39061 gctactgtac cttcttctac cataacttct tcaacagtac cagatactgg tgatgggatt
    39121 tctactactg atttatcgtt ttgtacctca gctaaaacat cgtcttcttc aatagtatct
    39181 ccagctttaa caaaccattt tacaatttca ccttcgtgga taccttcacc gatatcgggt
    39241 aatctaaatt caaatgccac gtttttgccc tcctaagatt tcgtaaatta aacatttcat
    39301 tttaatcaat gagactaaaa tatgctaact tcgttaactt ttaaaatgta ttaaaattct
    39361 aaagtttctt ttgctttttc gatgatgtca tttttgtttg gtaaccaaac attttcagct
    39421 tgagtgaatg gataaattgt atctgctgct gcaactcttc caataggtgc ttctaatgaa
    39481 aggattgcac gttcacttaa ttcagctaca actgctgcac caacacctgc ttgacgttga
    39541 gcttcttgaa ctacaactgc acgaccagtt ttttcaactg aagctacaat tgtgtcaaca
    39601 tcgattggtt gaacagtgcg caagtcaatt acttcaacag aataaccatc tttttcaagt
    39661 tcttctgcag ctttcattga ttcttgaacc attgcaccgt atgtgatgat tgaaatatca
    39721 ttaccttctt ttttcacatt agccttacca atgtcaattg tatattcttc ttcaggtact
    39781 tcttcacgga atgaacgata caatttcata tgctctaagt atacgactgg gtcattactt
    39841 ctaatagaag aaattaataa acctttcgca tcgtatgggc ctgaaggaat aacaaccttt
    39901 agaccaggag attgagctaa aataccttct aagttatctg cgtgcaattc aggtgtgtgt
    39961 acgccaccac caaatgggct acgaattgtt acaggtgcag ttttagtacc acctgaacgg
    40021 aaacgagtac gtgcaatttg tccagcaatc gcatcaaata cttcgaatac gaaacctaag
    40081 aattgtactt ccataaccgg acggaatcct tcaactgcaa gacccattgc taaaccacca
    40141 atacctgatt cagctaaagg tgtatcgaat actctatctt caccaaattc tttttgtagt
    40201 ccttcagtaa cacggaaaac accgccgtta acaccaacgt cttcaccaaa aattaaaaca
    40261 tcttggtcat ttttaagttc agttttaagc gcatcattaa tcgcttgaac cattgtcatt
    40321 tgtgccatgg cttacttcga ctccttctct ttgtaaattt catattgttc tgctaagttt
    40381 tgaggcatat cttcatacat aatttccatt agagaagtaa cagtttgttt ttcagtgtta
    40441 tcagcctctt taattgctgc tttaatatca gcttttgcac gttcaataac ttcattttct
    40501 ttgtcttcat tccataaacc tttgttttca aggaatttac ggaaacgtac taatgggtct
    40561 tttttctccc attcagcatc ttcgtctgaa gttctgtaac gagttggatc gtcaccagcc
    40621 attgtatgag gaccgtaacg atatgtcata gtttcaatta atgttggacc ttcacctgca
    40681 actgcgcggt cacgtgcttc tttagttgct tgatatacag ctaacgcatc cataccatca
    40741 acttggatac caggaatacc tacagcaatt gctttttgag ctaatgtttc agcagcagtt
    40801 tgcttgcttc ttggtgttga aattgcatag ttattgtttt gaataacgaa aattgcaggt
    40861 gctttataag ctgctgcaaa gttaatacct tcgtagaaat caccttgtga agaaccaccg
    40921 tcaccagtgt aagtgattgc aactgcattt ttaccacgtt ttttaagtgc aaatgcaacg
    40981 ccagcagttt gaatgtattg tgcaccgata ataatttgtg ggcttaatgc attaacgcct
    41041 tcagggaatt gatttccttt gaagtgacct cttgagaata agaaagcttc agttaatggt
    41101 aaaccatgcc aaataatttg aggaacatct ctgtatcctg gtaaaatgta atcttctttt
    41161 tctaaagcgt attgtgacgc taattgtgat gcttcttgac cagcagttgg tgcatagaaa
    41221 cctaaacgtc cttgtctgtt taatgagata gaacgttgat caaggatacg agtccatacc
    41281 attctttcca ttaattctac taattgttca tccgtaagat caggtactaa gtcttcattt
    41341 acgacgttac cattctcatc caaaatttga accatttcaa atttcgattg agtatcattt
    41401 aaaactttta ctgcatcgaa ttgggcttgt aacttaggag ccattcaatt caccatacct
    41461 ttcccataat tgtatagaaa ttcattttat ccttactaat tgtaccacaa ataaatggga
    41521 ctgttaaacc atttgaaaaa gttctgtttt aataaaaaaa gatacagata ataggaactg
    41581 tattagttgt ttcctgccat ctgtatcata ttaattattt aaattgatca acgtcttgct
    41641 tttctttacc aaccttattt agtactttcg tatacttatc tgatacttct tttaactttt
    41701 tatagttctg ttctattgct tttgattttt cgttaacacc ttgttgtgtc gcgtcatttt
    41761 gatttaaata tttaaataat gttttctctg agtttacagc ctttttatat gctttcgcgt
    41821 aatcactgtg taacttatat ttttctttta atacatcatc taattgtttt acttctttac
    41881 gtttaacatc attatcaatg ttatcaacgt gactttttgc tttcttaaag tcttgttcag
    41941 acttcttaat tgcgtcttct tctttttcaa attcctttag acgatcatcg gcatttttaa
    42001 ttaagtcttt tactgcttta ccgcgaactg ctgtatcttt actattgaca tcttcagtta
    42061 attttttctt tttctcatct aattcagcaa ttttcttacc aacagtttta attggttctt
    42121 catcgtcttt aattttatcc acttgcttta aatatgcctt aatttctttt ttatccgtag
    42181 tacatcctgc aagcaagaca ctagatgcta atactactgc gattgttttt ccaaatttca
    42241 ttacttgtac ctccacttgc tatttaaatt aaatgataaa taaaatctgc ttggaaatca
    42301 attttaaaca attacattac acatcttcat tactaagtat atctgttgac agttacgtca
    42361 tttataatat aatttttaaa gtcaagatga taaataaatt tctatagtat cattgcttga
    42421 aatcaacact ttaatcattt tttaggcaat tagcatatct gaaaatttac atatactatc
    42481 taaaataaac cttggcagta gttggttaac tttgtacata cttacacata aaaatgtatg
    42541 agtatcttaa caaccttttt actgtcattc aactaaaaca aattaaaata aggaaggtgc
    42601 aatatatgtt aacaatgaaa gacatcatta gagatggtca tccaactttg cgtcaaaaag
    42661 cagccgagct agaattacca ttcactaaag aagaaaaaga aacattaatc gccatgagag
    42721 agtttttagt aaatagtcaa gatgaggaaa tcgcgaaacg atacggttta cgttcaggcg
    42781 ttggtttggc tgcacctcaa attaatattt ctaaacgtat gattgctgtt ttaataccag
    42841 atgatggcag tggcaaatct tatgactata tgcttgtgaa ccctaaaatt gtaagtcata
    42901 gcgttcaaga agcttattta cctactggtg aaggttgcct tagtgtcgat gataatgttg
    42961 ctggtctagt tcaccgtcat aatagaatta caattaaagc caaagacatc gaaggtaatg
    43021 atatacaatt acgtctaaaa ggatatccag caattgtttt ccaacatgaa attgatcatt
    43081 taaatggtgt aatgttctat gatcacattg acaaagacca cccattacaa ccacatacag
    43141 atgcagtaga agtttaaaac acactattac taattatcaa agcttaggat aaatatgatg
    43201 tcctaagctt tttcttgtac aactttttcg aataaacaac agttaaatat attcaccttt
    43261 ctaccaaact ttttatcccc tcatttaaat tttaccgttt catataaaat cctttaattc
    43321 tttcttaaca ttaatttata atctctacat ttattttaat aaatagaact gcacatttat
    43381 tcgaaatact tagatttcta gtgagataaa ctgctttatt tattatcatt catcatgtaa
    43441 aataagattt aactgaaatt ttagtgttat ttcactaatt ttttaaaatg aacgacatga
    43501 tgaacctagt tattaaccaa atcgttatta agttacatta tagagatgat tggaatgaat
    43561 ttatcgatat atactccaat acgattttac tagggttaac aataaattaa acaaacattc
    43621 ttaggaggaa tttttaacat ggcagtattt aaagtttttt atcaacataa cagagacgag
    43681 gtaattgtgc gtgaaaatac acaatcactt tatgttgaag ctcaaacaga agaacaagta
    43741 cgtcgttact tgaaagatcg taattttaat atcgaattta tcactaaatt agagggcgca
    43801 catttagatt acgaaaaaga aaactcagaa cactttaatg tggagattgc taaataatga
    43861 aacaattaca tccaaatgaa gtaggtgtat atgcacttgg aggtctaggc gaaatcggta
    43921 aaaatactta tgcagttgag tataaagacg aaattgtcat tatcgatgca ggtatcaaat
    43981 tccctgatga taacttatta gggattgatt atgttatacc tgactacaca tatctagttc
    44041 aaaaccaaga taaaattgtt ggcctattta taacacatgg tcacgaagac catataggcg
    44101 gtgtgccctt cctattaaaa caacttaata tacctattta tggtggtcct ttagcattag
    44161 gtttaatccg taataaactt gaagaacatc atttattacg tactgctaaa ctaaatgaaa
    44221 tcaatgagga cagtgtgatt aaatctaagc actttacgat ttctttctac ttaactacgc
    44281 atagtattcc tgaaacttat ggcgtcatcg tagatacacc tgaaggaaaa gtagttcata
    44341 ccggtgactt taaatttgat tttacacctg taggcaaacc agcaaacatt gctaaaatgg
    44401 ctcaattagg cgaagaaggc gttctatgtt tactttcaga ctcaacaaat tcacttgtgc
    44461 ctgattttac tttaagcgaa cgtgaagttg gtcaaaacgt agataagatc ttccgtaatt
    44521 gtaaaggtcg tattatattt gctaccttcg cttctaatat ttaccgagtt caacaagcag
    44581 ttgaagctgc tatcaaaaat aaccgtaaaa ttgttacgtt cggtcgttcg atggaaaaca
    44641 atattaaaat cggtatggaa cttggttata ttaaagcacc acctgaaaca tttattgaac
    44701 ctaataaaat taataccgta ccgaaacatg agttattgat actatgtact ggttctcaag
    44761 gcgaaccaat ggcagcatta tcaagaattg ctaatggtac tcataagcaa attaaaatta
    44821 tacctgaaga taccgttgta tttagttcat cacctatccc aggtaataca aaaagtatta
    44881 acagaacgat taattccttg tataaagctg gtgcagatgt tatccacagc aagatttcta
    44941 acatccatac ttcagggcat ggttctcaag gtgatcaaca attaatgctt cgattaatca
    45001 agccgaaata tttcttacct attcatggtg aataccgtat gttaaaagca catggtgaga
    45061 ctggtgttga atgcggcgtt gaagaagata atgtcttcat ctttgatatc ggagatgtct
    45121 tagctttaac acacgattca gcacgtaaag ctggtcgcat tccatctggt aatgtacttg
    45181 ttgatggtag tggtatcggt gatatcggta atgttgtaat aagagaccgt aagctattat
    45241 ctgaagaagg tttagttatc gttgttgtta gcattgattt taatacaaat aaattacttt
    45301 ctggtccaga tattatttct cgaggatttg tatatatgag agaatcaggt caattaattt
    45361 atgatgcaca acgtaaaatc aaaactgatg ttattagtaa gttaaatcaa aataaagata
    45421 ttcaatggca tcagattaaa tcttctatca ttgaaacatt acaaccttat ttatttgaaa
    45481 aaacagctag aaaaccaatg attttaccag tcattatgaa ggtaaatgaa caaaaagaat
    45541 caaaaaataa ataattaaaa agctactaac tttgaagtga agttttaatt aaactcacca
    45601 cacattttta gtagcttttt ctttatatat gatgagcttg aaacataaat caatgttcaa
    45661 tgctctacaa agttatattg gcagtagttg actgaacgaa aatgcgcttg taacaagctt
    45721 ttttcaattc tagtcagggg ccccaacaaa gaagctgacg aaaagtcagc ttacaataat
    45781 gtgcaagttg gggatgggcc ccaacaaaga gaaattggat tcccaatttc tacagacaat
    45841 gcaagttggg gtgggacgac gaaataaatt ttgagaaaat atcatttctg tcccactccc
    45901 gattatctcg tcgcaatatt tttttcaaag cgatttaaat cattatcatg tccaatcata
    45961 attaaaatat cacctatttc taaattaata tttggatttg gtgaaataat gaactctttg
    46021 cctcgtttaa ttgcaataat gttaattcca tattgtgctc ttatatctaa atcaatgata
    46081 gactgccccg ccatcttttc agttgctttc aattctacaa tagaatgctc gtctgccaac
    46141 tcaagataat caagtacact tgcactcgca acattatgcg caatacgtct acccatatca
    46201 cgctcagggt gcacaaccgt atctgcccca attttattta aaatctttgc atgataatca
    46261 ttttgtgctt tagcagttac tttttttaca cctaactctt ttaaaattaa agtcgtcaac
    46321 gtacttgatt gaatattttc accaattgcc acaatgacat gatcaaagtt acggatacct
    46381 aaacttttca taactgcttc atctgtagtg tctgcaacaa ccgcatgagt agcgatatca
    46441 ctatattcat tcactctatt ttcatcatgg tcgatggcca ttacatccat gtctaatgca
    46501 ttcaactcac gaacgatact acctccaaaa cgacctagac cgatgactac atattcttta
    46561 cccatactcg ccctccatta aatgattttc atcaattcat tgaaaatata aatttaaaat
    46621 tattataaat gagtacccca actaaattat ctaaatgcag taatgcaagt aaatgaaagt
    46681 tggggtatct tctcaactta tgatttcttt ccttcaacat attctttgtc gaaaacaaat
    46741 aatcttaata ataatattaa cgatggaagt aataaaagta aacctaaaat aaagacaatc
    46801 actaatgtcc agcccatttc tggattaaca taagcatctg taatttttac aaacggatat
    46861 aaaaggtatg gcaatttact aattccatag ccaaagaaag cgaacatcat ttgtaaaata
    46921 acaaatacaa aagccaaacc atgctttttc ttaaagaatg ttaacaatga agctaatgca
    46981 aagaataaga aacttatagc aaacaaccac cagtagtcaa aaacagctga ataaaaatgt
    47041 tcagaatttt gaatgcgtaa tgatagaaat acgaataaac aaatgataat catcggtggc
    47101 cctaaaaata tgtgccattg tcttgttaaa ttatatgctg gttcgtcatt tgctttttta
    47161 gcataatatg tcaaaaatcc tgatgaaata tataaaactg aaataattgc caagaatact
    47221 acagaccaag caaatgggct taatagtaac tgcacccaat ctagatcgat aacattgttt
    47281 cgaacattaa tatagccacc ttctgtaata gttaaagcag ttgataatga agctggaatt
    47341 aataatccac ttaccccata catgaacatc catgctaatt ttgtatcttg accataattt
    47401 tcaaatgcat aaaaactgtt tctcaacgat atcataatca gtgctattga acctggtatt
    47461 aacaataccg tgcctaaata tttgattgac tctggaaaga aacctacgaa tcctacgaag
    47521 aagaaaacaa agaatacatt cgtaacttcc caaactgggt tcaaataacg tgaaattaag
    47581 tgattaattt tcttttcatc accagttaac tttgaatgca atgcgaagaa acctgcccca
    47641 aaatctatag aagcaataat gatatagcaa aataaaaaca accataacac tgttatacct
    47701 ataaatgcat aaatcatttt tctatttctc ctccttgctt cttggctaag cgatttacat
    47761 cttcatacgc cggtttattt ttaaacatac gaattaatac gtatgcacat gtatacatta
    47821 aaatgatgta caatatgcca aataaaattg taacgaaggt tattccgcct gcttgtgttg
    47881 ctgcttctgc cacgcgcata taaccacgaa caatccaagg ctgtctaccc atctctgtta
    47941 agaaccatcc aaattctata gctagcattg aagctggtcc ggttaataat attccgtaaa
    48001 gcatccattt atgagtagaa aactttctaa gctttttaaa cattaaagtt aagacataaa
    48061 cacctgaaat gacaaaacat aaaattccca tcgttaccat taaatcaaag aaataatgga
    48121 caatcatagg cggatgtaaa cttttcggaa aatcatttaa cccttgcact ttagttttga
    48181 cactattatc tgctaagaaa ctcaatagtc caggtaattc aatcgcacct ttaacttgct
    48241 gagtcttttc atctaacaca ccaaataata ataatttggc atgggaagat gtatcgaaat
    48301 gccattcata agctgctaat ttttcaggtt ggaatttatg caaaaatttt gcagataaat
    48361 cccctgccaa catagaaagt aatgttgaaa agaatccaac tatcatagac attttcaaag
    48421 ctttcttatg gtagacagta tctttaggtt gacgattacg caataattta aaagctgcta
    48481 ttgatgcaat aacaaatgcc atcgtcatac cggctgtagt aattacgtga aatgatcgaa
    48541 ctataaacga tgggttaaac atcgcttcta taggttgaac attgaccatc tttccattct
    48601 tcaactcaaa acctgcaggc gtattcataa atgaattcac tgaagtaatg aagaatgctg
    48661 agaaagagcc accaataatt actggtatac taattaagaa atgtgtccat ttatttttaa
    48721 aacgatccca agtatataaa tatatactta agaaaatagc ttcaaagaag aacgcaaatg
    48781 tttccataaa taatggaagt gcaataacgt gtccacccat ttccataaat gtaggccaaa
    48841 ttaatgataa ttgaagtcct ataattgtac ctgtaacaac tcccactgct acagtaattg
    48901 tataagcttt agcccatctt ttggccatag ctatatattg aagatcattt ttgcgaatac
    48961 ctaaaaattc tgcaattgcg aacattaaag gcataccaac accaatcgtt gcaaaaatga
    49021 tatgaactgc taaagtcata gctgtcaaaa accgactgat ttcaactgta tccatttaaa
    49081 aacatcacct ttttcttttt ttgatgacaa cacaatgaac ttaattataa ttgctataat
    49141 gtgtattttt aaaacattat gcgctatgaa attgtgtata atttatgtca gttcacaatg
    49201 tgttcacaaa tttgaatttc aatgtataat tgtgtatatt acattagaat aaagcacgaa
    49261 ggagcatgat acatgtcaga aataatcgtt tatacacaga atgattgtcc accttgtaca
    49321 tttgtaaaaa attatctaaa tgagcgtcaa attgattttg aagagagaaa tatcaacaat
    49381 caacaatatc gaaacgaaat gatagatttt gatgcttttt caactccgtt tattttgttg
    49441 aatggcaatc caatgtacca tgttgatctt gatgaaatca acaaagtatt aaatatccaa
    49501 gattaatttt catctaaatc aacttcatca tttttagcta tcataaatta aatatcgatt
    49561 tttaaattaa ctaacattaa ttaaagaaca atatttatgt caatggcacc tagttgttta
    49621 attagttagc aacaacattc cattgaaatt aacataaatc aaaaaaaaga acccgaagta
    49681 ttcataatta ctcacaatga atacttcggg tttatttata attctgtctt tcgaaagaat
    49741 taattatttt acgtagttgt taactaattc aataacttct tcttgcgttg cacagtcgac
    49801 tgcacggtta gcaagttcag tcatttcatt tttacttaaa ccattaattt gtcttcttgc
    49861 tttcagaata gacgttgcac tcatagagaa ctcatctaaa cctaaaccaa gcaataatgg
    49921 aatagctgtt tcatctccag ccatttcacc acacatacct gtccatttac cttctttatg
    49981 tgacgcttca ataacttgtt taactaaacg taagattgaa ggattatatg gttggtatag
    50041 atatgataca cgctctgaca tacggtcagc agctaatgta tattgaatta aatcatttgt
    50101 accgatactg aagaaatcta cttctttagc aaagacatca gctaatgctg ctgttgcagg
    50161 tatctctacc atgattccta attctatatc atctgaaatg tcatgacctt catttttaag
    50221 gttttctttt tcttctaata atatagcttt agcttctcta aattcgttaa ttgttgcaac
    50281 cattgggaac atgatattta acttaccata aactgatgca cgtaataatg cacgtagctg
    50341 tggtctgaaa atatcttgtt gcgcaaggca taaacgaatc gcacggtaac ctaagaatgg
    50401 attcatttct tcaggcaagt ttaagtatga taattcttta tctccaccta tatctaaagt
    50461 acgtacaaca acacgtttac cgtccattgc ttctaatact tctttataag cttcaaattg
    50521 ttcttcttct gtaggcattt ggtcacgacc catatataaa aactcagttc tatataagcc
    50581 gataccttgt gcaccatttt caataacacc tggcaaatca ttaggtgtac caatatttgc
    50641 agcaagctct gcgtgaacac catcaactgt aacagtatca gcatcacgta gtttttgtaa
    50701 ttctttcttg tcagcaaaat aacgctcacg tttatcttga taagcgatta actcatcttc
    50761 agttggatta acgattacat caccatttaa tccatctacg ataatcatgt cgccttgttt
    50821 aacttcttga gtaattgatt ttgtaccaac aattgctgga atttctaaag aacgactcat
    50881 aatcgcagag tgacttgttc ttccgccaat gtttgtagca aaaccttgta cgaattcttt
    50941 atttaattga gcagtatctg atggcgttaa gtcattccct acaataacaa cgctttcatc
    51001 aatcatactc ggattcggta attctacacc taaaatatgt gataacacac gtttagaaac
    51061 gtcgcgaata tcagccgcac gttctttcat gtattcatta tccatagatt caaaaatcgt
    51121 aacaaattgt gttgttacat ctgttaatgc tgaagcagca ttagcgtttt catttttaat
    51181 cttatcttga attggttgaa ttaattcagg gtcatctaaa actaataaat gtgcatcaaa
    51241 gatagcagct ttatcagcac ctaattgaac ctctgcatta tttctaattt tagttaactc
    51301 aactttagaa gcttcgatag cgttattgaa cttagcaact tctccttcaa catcagtgac
    51361 tttttcattt ttgtcgaatg ttaagtctgg ctcaactaac aaataagctt tagcaattgc
    51421 aacaccatca gatgcagcaa ttcctttaat taatttagac atgattattt agtcaatcct
    51481 tcttttgata agacgtcact gattgcttga atggcgtcag attcatcact accgtcagca
    51541 taaattgtaa tttcagcatc tttaccaaca ccaaggctca taacacccat gattgatttt
    51601 aagttaacct ttttaccgtt atattctaat tggatatcag aatcgaattt tgaagctgtt
    51661 tgaactaaca ttgttgctgg tctagcgtga ataccagtct cgtcgatgat tacatatgaa
    51721 ttttgttcca taattacatt tctccttcgt atagttagaa tcttcaatat tattacaata
    51781 ctaacttagt tttatacctt aactttacca aaaccatgct ttaaattcaa ttcgtttaaa
    51841 accggtttca gtaaaatttg caaattgcaa aactattata gcacctacat gacattttta
    51901 ataacatttt cgcatttttg aatgtgatct tttccaattt tattcataaa atagtaacta
    51961 atagatgcag acacagcttg tcctacaaca ggaaaccatt ttgtttgttt agctgcagtt
    52021 cgttttgcta catctctaat tacaattttt aaaaatgcac ttgaaattct tttaccaata
    52081 aattgactac cttgaattgc cgctgcagac ataattcttt ctttcacatc atccccaagg
    52141 ctattaactt gcttatgatc taatccgtat attttattta catcttcgat aatatctttc
    52201 attaatttta aatcaacacc aaagtctaat ccaggaattg gaacaacgct cattgttgat
    52261 gacattaaag actttctctt tacaattgct tcagcacgtt cgcgtctttc tctcaattct
    52321 tgaatcgtcg ttggcattgc accttttccg tcaacatttt ctattttaaa aactgaatta
    52381 ccaattttat ttgttaaatt tgatgttaaa ttatttttga atcccattgt atcatcaccc
    52441 ttctttttct caataagata ctatacccct ttttatcaaa ttaatgtcta atatttacaa
    52501 aaacagcttt taaatattta gatggcttat aatgtggatg tgttttaaaa tcttttggta
    52561 atcccataac ttcagttaat tcataatcaa cgccactctc ttcaagcgtc tttttaatag
    52621 tatttttaaa ttgctttaat ggatatacac ttgcgtttgt acataataat aatgttcctt
    52681 cagatgataa gatatttaag gcgccagtaa ttaatttgtc ataatctttt tgcactgaaa
    52741 atgtacgttt tttgttgcgt gcaaagctag gtggatcaat cacgatcgtg tcataactat
    52801 gtccatggcg tgtagcatat ttatagaaat caaaagtgtc catgacataa atatattgag
    52861 atttaggatc aatagcattt aatccaaaat tttcttcagt taaactacga gaacgattag
    52921 ccaaatctac acttgttgtt gaagatgcct cacttgctgc tattacagaa aaagcacctg
    52981 tataactaaa taagtttaaa acatggcgtt ctttggcata ttgatccctt aatttcttgc
    53041 gcacttcttt ttgatctaaa aaaatacctg tcatcaaacc atcttcaagg tctacattat
    53101 aaaatgtgaa gttttcttcg ataacaattg gaaactcagg tgcatcgcct tcaacaaaac
    53161 caccactata ttcgctgtct ttaaacctta ctttttcgta tatagattta taatcaaata
    53221 cttttctaac cgcttcaaga atggcatatt taaatttata aatacctttt gagtaccatt
    53281 gaatcaacaa atgaccatcg taattgtcga tcgttaatcc cccaattcca tcaccttcgg
    53341 cattaaataa tctaaaagca tttgttctat ctatattaaa ataataatca cgttctgcta
    53401 atgcagtatt aaacaatttc acaaagaaag ctgtgttgac ttcttgagct ttatcgtatg
    53461 ttagaaccca acctaatcct ttatgttgac gaccaacata agccgttgca acatattgtg
    53521 atttatcagt cacaatttga aaaatatctc cttcttttaa atgatcttgt gaatagatat
    53581 cctcttcttc aattaaagga tatccattaa aatattttgt ttctttgcct ttgttcagag
    53641 ttgctatttt catctgacaa cttcctttct gtgtttcgtt gtagttttat atagattaaa
    53701 ttgtttgcca aatttcaata tcatctactt ctaatcttac tgttgatttt aacaaattag
    53761 ttattcgaat gatatatgtt aaatttctat aaatgagata ctatatatcg tttcgcaaat
    53821 tccacttgtc acttcaacta tttactagtt ttcatgattt tgcaaatgga taacttttca
    53881 actattttat ttgcactaaa actttgtata caattttcaa tacttaattc acattacccg
    53941 aatcaatcaa atctagttac aaaaaaagta ctatccataa acttcattga cctgtatcta
    54001 agtaagttag agatatatct aacttatagc gtacacatca aaatgtttaa tagatagtac
    54061 ttttataatg cttttaatta tggtcgctct cgtaattgta acgaataaga aatgatgatt
    54121 gccaataaca ttaatagtaa tgttggcgta atgaacactg aaagtagaag tccatgaatc
    54181 attatattta taaattccgt taacaattta aagaataaaa tgctccccat aaataattgt
    54241 aaataataaa acttaccttt caagtggaaa gttaacgttg tagcgatata tgcgataata
    54301 atgaatagca gaattagtaa tgtaacccat tcaataacat attgtgtctg tgataatttc
    54361 caatcaccag atacaaataa tgcattacga ttattaaatt ctaaaaatgt gaataaaaag
    54421 atgacgacac cacaaaatag ttcgacatac atcggtttaa cccatttcgg ttttctcatc
    54481 caatcaggaa tatcgtcctc ttgaatatcg ataatatcat taactttctt tttgaattta
    54541 tctcttttag ctctaatttt atctaaatcg acacttcttc ttgtcttacg tgcatattct
    54601 gtagtcttat cttggagttg cttcaagtga attttagttt gattcgtgac attcccttta
    54661 catgttcttg ataattctgg ctcctcttca cctaattctt gctttgttaa aggtaaagct
    54721 cggcgcaaga acaagaaatt ccaaatagcc atttgaccaa gtaaccacat aattataaag
    54781 aaagcattga cacttaagat atcaattgac tctatttcat tagcatcaat tctgccatac
    54841 aatgcaattt gtgtaattaa ataacttata ccataactta caataatcca catgtaatga
    54901 tttttaacat tttcctgatt caattcatct ttataaacga tatatccaat atgaattgta
    54961 aatggaatga tgaaaattac cgcaaagaat ccatatactt gcgtcattaa tataagcgta
    55021 ataataaagt acatgatacc gataactaag aaaaagatag aaatatcata gtcatactta
    55081 atagttctaa gtaatgtatg accaatcaac ataactaaaa taccgaatat aattatcaca
    55141 attgcactaa ctatagggaa attacctata aatcgactca ttactttaat aatttcactg
    55201 aaaaatgcat tcagaaattc ccacacatta tgaacatgtg tatcaacttt tttaccacca
    55261 tttaattgtt taattaaagg caagttaatt aaaataatga gaccagtgaa gagtgaaacg
    55321 ataccgatta aatagctaaa aataatttcg gcgtgtttcc taagaaaaga cattatgttc
    55381 acctcaaact cattatatat taatctaatg ttgttgtctt tagataaatg aataaatagt
    55441 gctatagact tagaaaagtt taacgaaagt acaaccgggt aagatttata tataaataat
    55501 tatccaattt gggaggaatt aatcatgtca aatgaaaatc aaaataaaaa agcagcagaa
    55561 aaagctaaag aagttgaaga aaaactaaaa gataaaaaag aagaaaaaac agaagatatt
    55621 aatcaaacaa aacaagatat ccaagataca ctaaattaat ttaaatcccc ttacaacttt
    55681 tcgttgttag gggattttat tcatgcgctc ggcgcaagta tccctctttt accttacaaa
    55741 caataattcg taagccatta tgcacgggtt gttcactgtt gttaaagagg tatgattgat
    55801 aagtatccct cttcacttat caacttaata ttattacatg tcacatattt ttatatcttt
    55861 aattactaac atttctacta catctttaat ttaaatctaa tgctaatatt ataaatttta
    55921 aatcgaatta aacgaaattt catgaagaca acctattgac ataagtttat aaacgtctta
    55981 cagtataatt aattaaataa atcgcacaca ctaggggtgt tttatactga gatgaggctt
    56041 gccctcaaac cctttgaacc tgatctagct tgaactagcg taggaaagtg ttactataca
    56101 tatgttttac taatatatat tgtgaacgca taactttcct atggatggtt gtgcgttttt
    56161 ttattaggag gatgtaaaaa tgtcaaaagg tttaaagcta tctgaaattc tagtaaccgt
    56221 acttatttca gtagtattcg cagttattta caatttatgg tggtttgttt acaatggtgt
    56281 gcaagctaca ggccttcacc tcgaacaatt aacaaacggg gtttggttca tggcagcgat
    56341 tgtctgctat ctaattattc caaaaccagg tattgcttta ttggctgaat ttgctgcagg
    56401 tgccggcgaa actatcatta tgggtcgttt cgatattccg acaatcgttt acgctttcat
    56461 acaaggttta gcgtgtgaac ttgtctttgc aatttttaaa tatcaatctc gttcagttat
    56521 ggtagctatg ttagcaggtt tttgtacagc tatcgcggct ttcccgatcg attatttcta
    56581 tggttattta aatgaagttg caggttggaa cttaacatta ttcatcgtat ttagattaat
    56641 tagtggtgca gtagtcgccg gcgtcctatc ttacctttta gtaaaagctt tagataagac
    56701 aggcgtaaca aaattattta gaccagctgc taaagaagat tacgacaact tataagagga
    56761 gcattcagag tgttaaaagt aagtgattta cgattaaaat atccaagtgg tcagcgtaaa
    56821 attttcgatc atttaaatat cactattcaa gacaaagaaa aagtactttt actcggtcct
    56881 tctggttgcg gtaaaagtac acttctgaat gtattaagtg gtattgttcc taatttaatt
    56941 gaattaccta tgaaatatga tgaactaatc gttgacccat taagtggcgt tattttccaa
    57001 gaccctgata gccagttttg tatgccaaaa gtatacgaag aacttgcatt cgttttagaa
    57061 aatagacaag taccacgtga agacatggat gcgttaatta tcaatgcttt aaatatggtc
    57121 aatttaaatg ttacccctga aacgtatatc aaagatttaa gtggcgggat gaaacagaaa
    57181 ttggcaattg ttgaaaccat tcttcaacaa tcaaaaacat tgtttttaga tgaaccgaca
    57241 gcaatgttag atgttcaagc aacggaagat ttatggacta aactaattga actttgggaa
    57301 gatcaaactg ttgtaatcgt tgaacataaa gttaaacaca tctggaatca tgtcgaccgc
    57361 gtcattttga tggattataa cggaaatatc attgccgatg agtgtcctga aatcatatta
    57421 cagaagtatg ttcatttact cagtgaatat ggtgtgtggc atccacgtgc atgggaattc
    57481 gcaccaagtc gtgttgactt tccaacaaca aactcacact tattacaatt taaaaatgga
    57541 cgtattattc gcggtaaatc aacgttgctc tcattctcag atttagaaat tggtctaggt
    57601 gagtggatta caattacagg ggcaaatggt agtggtaaaa caaccttgct tgaatcaatt
    57661 atgcaattga ttaaatatca aggtgatgtt tattttgaaa atcagcgttt aacaaaaatt
    57721 aaacatgcag caaaacacat gtatctagtt tatcaaaacc cagaattaca atttataaca
    57781 aattcggttt atgatgaaat taacattcat tttaatcacc tttctaaaga tcaaagtgat
    57841 gatgaaacga tacaactttt aaaactttta gatttagaaa atgtaaaaga tcaacatcct
    57901 tatgagttgt ctattggtca aaaacgacgc cttagcgtag ctaccgcact aagttctaaa
    57961 gctgatatca tctttttaga tgaaccgaca tttggacttg atagccataa cacattccag
    58021 ttgatcaaac ttttccaaaa acgaattaat ttgggccaaa gtattgtcat ggttacgcat
    58081 gacgatgaaa taattgaacg ttacccatca agaagactta aaatttcaga tggtgcgtta
    58141 ctagattgtg atggtgatac gaatgtatga gcaatggaaa gttcggcaat catttgttga
    58201 tgatgtcaac attataacga aactatttct tggaattata ctatttttct ttattatatt
    58261 tgtgcataat ttcgatttta tgttttatat cactatctta atgttagttt tcttattgat
    58321 gtttaatggt actcagttta aaattacagc aatttttata actataagta ttggctttgc
    58381 attactctct tccctgttca tgattttata cgggaatggt agccatacaa ttttgaaatt
    58441 cggatttatc catattacaa cagaaagttt agttcgtggt ttacatgtat cattaagaac
    58501 cattgctatt tcatttttcg gtatcttaat cgcactcaca tctcaggttg tcatgatttt
    58561 ttatagttta atgcaacatt taaaggttaa atcaaaagtt gcttatgcat tcatggctgc
    58621 cattcgtatg atacctttaa tgattagttc tttaatacaa cttcgcagat ctttaaaaat
    58681 gcgctatcaa atgatagacg cagcaaatta tagaggatta aagcgattta agcatttaat
    58741 catacctcta ttaagccaaa acattagaaa ggctcatcaa ttatcagttg cgatggagtc
    58801 gaaaggattt agagatggtc ctcgtactta ctattacaac gtacctttct catataaaga
    58861 tgtaatcttt attattgttt taattgccat tattactttg gcatattatt gttcaatcac
    58921 cttacccatc acgggcatag atgatgttcg tcttggtcgt gtaggttaaa ttttatccat
    58981 aattgtatag atactaaatt agaaattcat tgtatagaaa aagaaaacca tttattccat
    59041 actaaacaaa agaggtgcca caaacatgtt attttgttca tccaaacatg ttgtggcgcc
    59101 tcttttgtat tacatatgca taatcacaaa tttggtacag aaatcaatgt cctagactct
    59161 acaactaaaa aagctatgaa aaaatctata gttatagatt cgttcatagc taatcttagt
    59221 atttttaaat atacttattt aagttgtagt gctttattcg caatgtcata acgatagaat
    59281 aaatggtcac tttgtatttg agatactttt tcgtatgcgt ctcgctgtgc atcttgtaca
    59341 ttgtcacctt ttccgatggc aagtataact ctaccacctg aagtaacaaa ggtatcacct
    59401 tgcttcttta atccactaac aaaatagttt tcatttaaat caaagccact tactttatgc
    59461 cctttttcat atgcatcagg atatcctttt gatgccaaca tgacccctac aatagattca
    59521 tttttccatt tgaattcagt acgttttcca tcatctaaat caataatatg ctgcattaaa
    59581 tcactttcca tgcgacttaa taatacttga gcttcaggat caccaaaacg ggcattaaat
    59641 tcaattactt ttggaccatc tttagttaga atagcaccaa tgtataatac accgaagaat
    59701 tgataacctt cattaagcat tgcctttgca atgggttgtg caattgtttc atttgtaagt
    59761 tttaaaacat cgtcactaat atgtggtact ggacaataag cccccatacc accagtattt
    59821 ggtccttcat catgatcaaa tgcgcgttta tgatcttgtg caatacagtc gaaaggaact
    59881 gctaattcac tattaacaaa tgtcattagc gagaactctt caccttctaa aaacgtttcg
    59941 aacacaacag taccttcttc ttcatcacca tacataatct caatagcact tctggctgct
    60001 tcaatagtat ctgcaataat aacgcctttc ccagcagcta acccatcttt cttgacaaca
    60061 acagggaatt cacagttttc aatatatgtt aatgcatcct tttttcgctc tacttcctta
    60121 taatcagcag ttggaatatt atatttttcc attatctttt tagcaaataa ctttgagcct
    60181 tcgatttgag ctgcttgctt atttggacca aacactttga aaccattcgc tcgtaaaatg
    60241 tctgctaatc catcaattag cggttgttct ggacctataa ctacccaatc aacattttgc
    60301 tgtttagcaa aatctagtat cccttgatga tcagattctg aaatttcagt gtgtacttca
    60361 gctataggtg tcattgcctc attacctgga atgacaaaca cttgtttaac tagattcgat
    60421 tgattaagtt tatatgcaag tgcatgttct cgtccaccag caccaattac taatacattc
    60481 atttatgtta cgccccttta aaattagtgt ttaaaatgtc gagtgcctgt gactaccatt
    60541 gcaataccat gtttattagc catatcaatt gaatcttgat ctttaatcga accacccggt
    60601 tggataattg cctttatacc atgttgtgct gcaagttcaa ctgtatctcc catagggaaa
    60661 aatccatcag atactaacgc cacatgatca ttgatttcaa tagctctttc taatgcaatt
    60721 ttagcagcac cgacacgatt catttgtcca gcacctattc ctacagtttg tttattatta
    60781 cttaaaataa ttgcattact ttttactgat ggtacaactt tccatcctaa taacattgct
    60841 tcccattgtt catcagtagg tgcaacttct gtaacaactt tcatttcttc ttttggcacg
    60901 acataattgt ctttatcttg aactaaatat cctccagata ctgaaacaaa ctcttcttcg
    60961 ttactgtcta tagtcatatc aatttctaat aatcttacat tcttcttttg ttttaaaata
    61021 tttaatgctt catctgtaaa ttttggtgca ataatgactt ccaaaaagat actatgcaat
    61081 tgctctgcta actcaggtgt tacagctcgg tttaatgcaa caattccacc aaatattgat
    61141 tgactatccg cttcatacgc atgttgaaat gcttgttcta tcgtgtcacc gataccaaca
    61201 ccacatggat tcatgtgttt aaccgcaact gcagcaggtg tatcaaactt tttaactaaa
    61261 gctagtgtag catctgcatc tttaatattg ttatagctta attgtttccc atgtaattgt
    61321 ttagcgcctg caatcgtgtg cttagcattc gaagttctca caaaatacgc tgattgttgt
    61381 ggattttctc catatcttaa agtttcttta tcccctttaa agaaacgtac aatcgcttca
    61441 tcatattctg cagtatgctc aaaaacttta atcattaatg attgtctata tgactcatct
    61501 aacgaatcgt ttcttaatcg cgtcaatact tcatgataat ctgccggatg tacaattgtt
    61561 gttacatgtt tatagttttt agctgcagca cgtaacattg ttggaccacc aatatcaata
    61621 ttttcaattg cttcgtccat cgtcacatca gggtttgcaa cagtttgttg gaatggatat
    61681 aaattaacta ctaccatatc aattaaatct atatgttgtt ctgataattc atttaaatgt
    61741 tgcggtttat ttcgatcagc taaaatgcca ccatgaacag ccggatgtaa tgttttaaca
    61801 cggccatcca ttatttctgg aaaatgtgtt aagtctgaaa cagaacgaac tggtacattt
    61861 gcttcatcta atatacgttt agtaccacct gttgaatata attcataatt taattgcgtt
    61921 aacgctttag caaactctac aatacctgtt ttatttgata cgctcaaaat agctttcttc
    61981 atcacataca tactccttat tttacaattt tagcaataac acttggatat aactcatatt
    62041 ccaatttttt tactttctct tctaattgtt cttttgaatc gtccggtcta atatcacatt
    62101 gtctctgttc aataatttct cccgtatcca taccactatc aacataatgt actgtcgaac
    62161 cagtaatagt atcgccacta tgatatgctt ggcctattgc gtcaatcccc ttatatttcg
    62221 gcaatagaga tggatgtata ttcaatattt taccttcaaa tgaatctaat aagtctggac
    62281 caattagacg catgtagcca gctaaaataa tccactctac cttatcttca tttaataatg
    62341 ttactaaatg ttgttcatat gctgcttttg aatcaaattg ttttggttca ttaatataaa
    62401 caggaatatc gtgctttttt gctctatcta tacaaaacgc attttgatga tccgtatata
    62461 gcgccgtaac ttcaatattt tcaagttttc ctgattcaac atgctcaact atattttcaa
    62521 agttacttcc tgaacctgat gcaaaaatcg caattttaac cattgttata cccccaacaa
    62581 ttcaattgca gttgactcat ttttcacaat atgaccaatt tgataggctt ccacattttg
    62641 ttctgctaaa atcttcaaag cgcgtgatac atctttttca tcaacgataa ccgtatagcc
    62701 aatacccatg ttaaaaatgt tatacatttc atttgtctct atattgcctt gttgttgtaa
    62761 ccaatcaaat atttttggcg ttggaaatga tgtagtatca attctagcag catatccggc
    62821 tggcaatgca cgtggaatat tttcataaaa acctccacca gtaatatgat tcattgcctt
    62881 aatagaaact tcttttttca aagcaagtac aggtttaaca tataatttag ttggttctaa
    62941 aaagacatct ataaatggac gattatcaaa gtttgatgcc aaatcaatgc ctgattcatt
    63001 aattaatttg cgcactaaac tgtatccatt tgaatgaatg ccacttgacg caagccctat
    63061 aacaacttgt ccctctttca cttctgaacc atctacatag tcatcctttt caactgctcc
    63121 aacagcaaat ccagctacat catattcgcc ttcgtgatac atttcaccca tttcagcagt
    63181 ctctccaccg ataagtgcag tattcgtttc aacacatgca tcactaatac ctttaacaat
    63241 ttgttcaata acttcaggaa caactttatt tgtagcgatg taatctaaaa aatataatgg
    63301 ttctgcaccc gtcgttaaaa tatcattaac acacattgca actgcatcga ttcctatcga
    63361 atcatgttta ccataatcga tagctagttt taatttcgta cctactccgt ctgttccaga
    63421 aactaaaact ggcgctgtca tatttaattg tgataaatca aatgtagcac cgaatcctcc
    63481 taaaccaccg ataacttctt tacgcatcgt acgtttaaca tgactagaca ttctttctac
    63541 agcttcataa ccagcatgaa tatttacacc agattgttca tatgctttag acatttaaat
    63601 ttccctcgct atcaaaaaag tgtttgtttt tagaaatata ttgcttttgt cgatgactta
    63661 aatgtgcttt ataatttgct tcataatcat ataaccctgc aggataatct ccagtgaaac
    63721 tttctacaca taagccacta taaggcgcgt cataatctaa accaattgat tcaattaacc
    63781 catctacaga tagatatgct aacgaatcag cgccaatata atctttaatt tcttcaggtg
    63841 atttgcttgc agaaattaat tctgccgtag ttgaaacatc gattccataa aaacttggga
    63901 acataaattc cggtgatgct atacgcacat gcactttatt tgcaccagaa tcttttaaca
    63961 ttttcacaat acgtcgaatt gtcgtacctc gaacaatgga atcatcaaca agaataatat
    64021 ttttaccgtc tacaatatct ttcactgcag atagcttaac tcttacacct tgttcacgta
    64081 actcttgcgt tggttgaata aatgttcttg caacatattg atttttaact agtcccattt
    64141 catatggcaa accgatttct tcagcataac cgctcgctgc agataatgat gaattaggta
    64201 caccgatgac catatcagct tttaccggac tttcttgtgc taacatttta cctgaagcct
    64261 tacgcactgc atgaacattt ttaccagcaa tcgttgaatc tggtctcgca aaataaatat
    64321 attccatcgc agaaattgca gttgtcgtat gatgtgtata agatttaact gtaatacctt
    64381 tatcattaat cacgacatat tcacccgcat gaatatcttg aacgaattct gcacctaaca
    64441 catcaattgc acatgtttca cttgcaagga tgtatgtccc atctttcatt ttacctacaa
    64501 caagtggtct gatagcattt ggatctactg cgccatataa cgcatccttt gttaaaattg
    64561 caaatgtaaa accaccttta atttttcgaa gactttcttt taatgcttct tcaaaagttg
    64621 gcgccttact gcgtcgaatc aaatgcataa taacttctgt atctgaagac gaatggaaga
    64681 tagcaccttg tttttctaaa ttttgacgta atgatttagc attaatgagg ttaccattat
    64741 gacaaatacc tacactcata tcataaaagt gatacagaaa cggttgaata ttttcaatac
    64801 ctttattacc tgaagtagca taacggacgt gaccaattgc atgttgatat ccttttaatc
    64861 gttccatttg gtcatcttta atcgcttcag ttagtaagcc taatcctcgc tcgcctttta
    64921 attcattttg atcagaaaca actatacctg caccttcttg accacgatgt tgcaaactat
    64981 gaagtcccat atatgttagt tgcgctgctt caggatgatt ccaaatacca aacacgccac
    65041 attcttcgtt taatcctgag tagttaaaca ttgagcaatt gccccttccc atatttgttt
    65101 aatatctgaa acattttcac taatctctgt atatggtgtt gttactttga aattatcact
    65161 atctgttaaa agaccaattt ctattgcatt atcaatattt aaagttttac ctgatttaac
    65221 agaaacaaca tatcggcctt gtgtctcact aaacaattgt gcatttgtta tatctattaa
    65281 agactttaat cctaaaccgt aatgcgcact tagtttagct aaggtaatca gtaagccacc
    65341 tttaccaacc gtttgaacat gtgataatac cccttgacga atagcggtct tgattgattc
    65401 acctttttta acttctgaac tcaaatctat agattcaaat tcgtgattta ctttgccata
    65461 aatcaatttt tcaagttgac taccaccaaa gtcgtcctta gtatcaccaa ttacatataa
    65521 cttatctcca acttgaggtt gaaaatcatt taaatagtct acgttttcaa tcagacctac
    65581 cataccaaca attggtgtag ggaaaataga agtgcctttc gtttcgttgt ataaagaaac
    65641 gtttccagaa acaacaggtg tcttaagtat gtcgcaagct tctgccatac ctttcgttga
    65701 atctgctaat tgttgataaa tttctttctt ttcaggagaa ccataattta agcagtcagt
    65761 cattgctaat ggtgttgcac ccacggcaat taaattacga taagcttcag ctactaccat
    65821 ctttccacct tcatatggat tgttatatac ataacgcgct tcaccatcaa ttgttgaagc
    65881 aattgcctta tttgtgcctt ccacacgtac taccgatgct tgaagtcctg gcttaattat
    65941 cgtattggca ccaacttgtt gatcgtattg atcatataaa tagtgtttag atgctatagt
    66001 cggatgctta agtaatttaa agaaagtatc tttaacatcg atgtgtgtat aatcattttt
    66061 agaagtatta taattttttt cttctccttc taaaatatat acaggtgctt catcagctag
    66121 tggttcaact ggaatgtcag cataaacttc gtcttcatat gttaaaacaa aacgatttgt
    66181 atctgttact tcacctataa cagcactatc caattcgtgc ttatcaaata aatctaagaa
    66241 tttttgttca gtaccttttt caacaactag taacatacgt tcttgagttt ctgaaagcat
    66301 catttcgtaa ggagaaatac ctggctcacg tgttggcact tgttctaatc tcaaatgtaa
    66361 accactacca ccttttgccg ccatttcaga agatgaagat gttaaaccag cagcacccat
    66421 atcttgaata ccaactaatt catcaaatgt aattgcttca agtgttgctt ccattaattt
    66481 tttacctaca aatggatcac cgatttgtac agaaggtcgt ttactttcgc tttcttccgt
    66541 caattcttca gatgcaaaag tagcaccatg aataccatct cgaccagttt tcaaaccaac
    66601 ataaatgacc gaattaccta caccttttgc tgtgcctttt tgaatcatgt cgtgattgat
    66661 aacaccaaca cacattgcat taacaagtgg attaccatca taacgttcat caaattcgat
    66721 ttctccagca gttgttggaa taccaatgca gttaccataa cctccgatac cctttacaac
    66781 acctttaagt aatctttggt tttgtttatt atctaattct ccaaatctaa gactgtttaa
    66841 caaattaata ggtctagccc caatagagac aatgtcacga atgattccac caacgcctgt
    66901 agcagcacct tgatatggct caattgcaga tggatgattg tgagactcta ctttaaatac
    66961 tacggcttga ttatcaccga tatcgactac ccctgcacct tcacctggac ccataagcac
    67021 atgctcacct gacgtaggaa attgctttaa aaacggttta gaatgtttat aagagcaatg
    67081 ttcactccac ataacagaaa agatacctgt ttctgtaaag ttaggttgtc tgcctaaaat
    67141 atcgcaaact ttttcatatt cttgatcact taatcccata tcttgatata ctttttcaag
    67201 tttaatttct tcaacgcttg gttcgataaa tttagacatg ttgttccctc caacttttta
    67261 ccatagcttc aaataatttc acaccactat cagtacctaa caacgtttct aaagctcttt
    67321 caggatgtgg catcatgcca catacattgc ctttttcgtt aacaattcct gcaatatcat
    67381 catatgaacc gttcggatta ttcacatatt tcagaataat ttgattgtta gcttttaatt
    67441 gttgatatat ttcatcagta caataataat gaccttcgcc gtgagctaca ggatatataa
    67501 ctttttcacc ttgttcataa agatttgtaa aagccgtttg attattcact atttctaact
    67561 cttcatttct actaataaat aaatgtgaat cgttatgcaa taatgcacca ggtaataagc
    67621 ctatttcagt taaaatttga aacccattac aaacacctaa tactggctta ccttcagctg
    67681 caagacgttt aacttcagaa ataatcggcg ctacactagc cattgcccca gatcttaagt
    67741 aatccccgaa tgaaaatcca ccaggaataa gtacgccatc aaatccactt agtgatgttt
    67801 ctctataatc tacatattcc gcttcaacac cacttttaat agcagcatta aacatatctc
    67861 tatcacaatt cgaacctgga aaaacaagaa ccgcaaattt cattttatgc attctccttt
    67921 tcatcttcta aaactttata gctatattct tcaatcactg tatttgcaaa caatttttca
    67981 cttagagttg taataatgtt gtgtaccttt tcatcactaa cctcatccac tgtcatatat
    68041 aatacttttc ctacacgaat atcattcact tgtgcataac ctaagtcatg tacagctcga
    68101 gtaagcgttt gtccttgcgt atctaatact tgtggttgta atgtgatatg tagttcaatt
    68161 gttttcatta ttttaaatcc tccaatttgt ttaaaaatat ttgatatgtt tcaatcagtg
    68221 atccagtgtt atttctatat acatctttat caaagtttgc attggtagct ttatcccaaa
    68281 ttcgacatgt atctggagat atttcatccg ctaacaaaat ttgaccagtc tcagtctttc
    68341 caaattcgat tttaaaatct actaatttta aattcatcgc atccattaat tgttttaata
    68401 cttgattaat ttctagcgct ttcgttttta atgtttcaat atctttatca gtcgcaatat
    68461 tcagtaattt gacgtgatca tctgtaatca aaggatcatt taaagcatca tttttataga
    68521 aaaattccac aagtggttct ctaaaaactt caccattttc aaaacctaaa cgctttgtaa
    68581 tagatccgct agcaatatta cgaacaacta cttctaatgg aattattttc acaggcttaa
    68641 ctaattgttc tgtttcagat aattgcttaa taaagtgact ttctattcca ttttcttgta
    68701 aatatttaaa tataatagaa gtaatttgat tatttaatcg ccccttacct gccattgtgt
    68761 ctttcttagc cccgtttcca gcagtaactt catctttata ttcaactctt aattcatttt
    68821 cttgatttgt tgagaaaatg cgcttcgctt ttccttcata tagtaatgtc atgctttaat
    68881 tactcccctc aaatttagcg tacatatctt gttcagtttg gtttacatca ttcgttagta
    68941 cagtcatatg ccccattttt ctgctatctt tacgctcaga cttaccataa atatgtaagt
    69001 gccactctgg atgttcatta aattcatttt ccaataaatc taagtcttta cctagtaagt
    69061 tcatcatgac tgcaggcttt aataattcaa ttgaatttgg taatgattgt ccggtaactg
    69121 ctaaaatatg agtatcaaat tgtgaataat cacatgcttc aattgaataa tgtccggaat
    69181 tgtgaggcct tggtgcaatc tcgttcacat acaattggtt gttactatct ataaaaaatt
    69241 caactgtaaa tgttccaatg aaatgaatcg attggataat tttatttact tgctctttcg
    69301 cctcagctgt tttatctatt ctcgctggaa caattgtttt gaaaagtatt tgatttctat
    69361 gctcattttc ttgtaatggg aaataagtga tttgattgtt gtttcctctt gtaacagtaa
    69421 gtgatacttc tttcttgata ttcaaatatt tttcagctac gcattcacta gtttcaatta
    69481 atttaaaacc ttcttgtaag tctttttcgt tgttaattaa aacttgacct ttgccatcgt
    69541 agccaccaaa tctagttttt acaataaaag gatatcctaa tgtttcaatt gctttgtcaa
    69601 tatctgtaga ttcttttact gaaatgaatg ggacaacctt ggtaccagca ctttttaatg
    69661 tttctttttc agttaagcgg tcttgtaata actgtatagc ttggtaacct tgcggaatat
    69721 tgtacttttc acataatagt tttaattgtt gggctgaaat gttttcaaat tcataagtaa
    69781 tcacatcaca tttttgtcct aattgattga gtgccttttc atcgtcatac ttggcttgta
    69841 taaattcgtg tgcaacgtat ctacatggac aatcttcagc aggatccaat acaaccactt
    69901 tataacccat tttttgagct gattgtgcca tcatctttcc aagctgacca ccaccaataa
    69961 tgccaatagt cgcaccaaac tttaatttat tgaagttcat tttgcatgtc ctccactttt
    70021 tgaattaacg aagattcata ctgatttagt ttttcaacta aagaaggatt ttgaatactc
    70081 aacattcttg ttgcaagtat acctgcattt ttagcacctg ctgcaccaat tgcagtcgtt
    70141 gcaaccggaa tacctcctgg catttgaaca atagataata aagaatctat accctttaaa
    70201 ctttttgttt caatcggcac tccaataact ggtagcgtcg ttaatgatgc aaccatacct
    70261 ggtaaatgtg ccgcaccgcc agcgcctgca atgataatgt ctatacctct ttctctcgct
    70321 tcagaagcaa attgaaccat catttttggc gtacgatgtg cggatactac ttgtttttcg
    70381 tacggaattt caaaataatc caacatgtta caactctctt gcataatttt ccaatcggaa
    70441 gaactgccca taatgactgc tactttcact ttgtacaccc tttcaaaagt ttgaattgtg
    70501 aattacttta gttgtatatt atagatatag cataacaagc aatttctgct ttttcaatca
    70561 aaaatcgaac tttattttga ttttttgttt gaatttacgt cttttgctat gtaaattagt
    70621 tttataaact aacaaagtta ggatattgac aataggagga gaagttttta tggttgctaa
    70681 aattttagat ggtaaacaaa ttgccaaaga ctacagacag gggttacaaa atcaagttga
    70741 agcgctaaaa gaaaagggtt ttacacctaa attatccgtt atattagttg gtaatgatgg
    70801 cgctagtcaa agttatgtta gatcaaaaaa gaaagcagct gaaaaaattg gtatgatttc
    70861 agaaatcgta catttggaag aaacagctac tgaagaagaa gtattaaacg aactaaacag
    70921 actaaataat gatgattctg taagtggtat tttggtacaa gtaccactac caaaacaagt
    70981 tagcgaacag aaaatattag aagcaatcaa tcctgataaa gatgtggacg gtttccatcc
    71041 aataaatata gggaaattat atatcgacga acaaactttt gtaccttgca caccgctcgg
    71101 catcatggaa atattaaaac atgctgatat tgatttagaa ggtaaaaatg cagttgtaat
    71161 cggacgaagc catattgtcg gacaaccagt ttctaagtta ctacttcaaa aaaatgcatc
    71221 agtaacaatc ttacattctc gttcaaagga tatggcatca tatttaaaag atgctgatgt
    71281 cattgtcagt gcagttggta agcctggttt agtaacaaaa gatgttgtca aagaaggagc
    71341 agtaattata gatgttggca atacgccaga tgaaaatggc aaattaaaag gtgacgttga
    71401 ttatgatgcg gttaaagaaa ttgctggagc tattacacca gttcctggtg gcgttggtcc
    71461 attaacaatt actatggtat taaataatac tttgcttgca gaaaaaatgc gtcgaggtat
    71521 tgattcgtaa agagcctgag acataaatca atgttctatg ctctacaaag ttataatggc
    71581 agtagttgac tgaacgaaaa ttcgcttgta acaagctttt ttcaattcta gtcaaccttg
    71641 ccgaggtggg acgacgaaat aaattttacg aaaatatcat ttctgtccca ctccctaata
    71701 actgagtttt aatgaactct tttaacccaa attaaatatt attttgcaat tgcaatgaat
    71761 aacaagaaaa atctgggaca ttaatcgatc aaatgctccc ttcaaagtag acattgaata
    71821 aatgaaaact ttgaagggag catttcacat tgatctaggc tcaacaattt tatatagaca
    71881 gtagttaatt gaatgaaaat aagcttgtaa caagcatttt ccaattatag tcaggggccc
    71941 caacaaagag aatttcgaaa agaaattcta caggcaatgc aagttgggtg tgggccccaa
    72001 cacagaagct gtgactaatg ataaaatact actacataat atattattag tggttcttta
    72061 tcatttttgc ctcccttttc ttattgttat gatgcacaac aatttaagtt caaactgtcg
    72121 aataaagtta tatttgattt caaattatcc ctgaattatt aattttacaa ttgtggcaga
    72181 ttttcaaaat aataattatt tcctcattat ttataaattt atatttaaat ttcattcttt
    72241 atagggtaat attaggacta tagtatgatg tgtaaataat ataaattaag gtatagtaaa
    72301 gctaactcag aaatgactta tcattcggag gttacattat gaataaacta ttacagtcat
    72361 tatcagccct cggtgtttct gctacactag taacaccaaa tttaaatgca gatgcaacga
    72421 cgaatactac accacaaatt aaaggcgcta atgatatcgt tattaagaaa ggtcaagatt
    72481 ataaccttct aaacggcata agtgcatttg ataaagaaga tggagattta accgataaaa
    72541 ttaaagtcga tggccaaatt gatacatcta aatctggtaa atatcaaatt aaatatcatg
    72601 tcactgattc agatggtgca attaaaattt ccactaggta tattgaggtt a
//
LOCUS       DAPIDM010000003        65412 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000003 DAPIDM010000000
VERSION     DAPIDM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 65412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.003"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..98
                     /locus_tag="RJE42_000134"
     CDS             <1..98
                     /locus_tag="RJE42_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875596.1"
                     /translation="TPTIACTYLTNSKNIVVFLEKIKHDNKNVKV"
     gene            409..1362
                     /locus_tag="RJE42_000135"
     CDS             409..1362
                     /locus_tag="RJE42_000135"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDV5875597.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(1683..3212)
                     /locus_tag="RJE42_000136"
     CDS             complement(1683..3212)
                     /locus_tag="RJE42_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV5875598.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTIKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMYFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMSDIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWLCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGEDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            3574..4509
                     /locus_tag="RJE42_000137"
     CDS             3574..4509
                     /locus_tag="RJE42_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDV5875599.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADEHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            4619..4722
                     /locus_tag="RJE42_000138"
                     /pseudo
     CDS             4619..4722
                     /locus_tag="RJE42_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4847..6943
                     /locus_tag="RJE42_000139"
     CDS             4847..6943
                     /locus_tag="RJE42_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDV5875600.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLEDHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVPCVIRRRIQSGHVINPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTTAIDQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QEENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKDVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQAFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            6928..7395
                     /locus_tag="RJE42_000140"
     CDS             6928..7395
                     /locus_tag="RJE42_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV5875601.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKLDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            7418..7696
                     /locus_tag="RJE42_000141"
     CDS             7418..7696
                     /locus_tag="RJE42_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV5875602.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            7923..9182
                     /locus_tag="RJE42_000142"
     CDS             7923..9182
                     /locus_tag="RJE42_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV5875603.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQRVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            9200..10255
                     /locus_tag="RJE42_000143"
     CDS             9200..10255
                     /locus_tag="RJE42_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV5875604.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            10257..10403
                     /locus_tag="RJE42_000144"
     CDS             10257..10403
                     /locus_tag="RJE42_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875605.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            10427..11470
                     /locus_tag="RJE42_000145"
     CDS             10427..11470
                     /locus_tag="RJE42_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV5875606.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESTNDVIVKVRATGICGSDTSR
                     YKKMGPYIQGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            11998..12714
                     /locus_tag="RJE42_000146"
     CDS             11998..12714
                     /locus_tag="RJE42_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV5875607.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNTQGIN
                     DDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            12707..13732
                     /locus_tag="RJE42_000147"
     CDS             12707..13732
                     /locus_tag="RJE42_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV5875608.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LPSSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            13754..15448
                     /locus_tag="RJE42_000148"
     CDS             13754..15448
                     /locus_tag="RJE42_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDV5875609.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFKCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDYREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            16176..17345
                     /locus_tag="RJE42_000149"
     CDS             16176..17345
                     /locus_tag="RJE42_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDV5875610.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NSYSTSIFQHAFHVSRNKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIEETANEYIEARTVFYQKFCSLEDGHVSQRICQTIFK"
     gene            17621..18337
                     /locus_tag="RJE42_000150"
     CDS             17621..18337
                     /locus_tag="RJE42_000150"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDV5875611.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            18330..19355
                     /locus_tag="RJE42_000151"
     CDS             18330..19355
                     /locus_tag="RJE42_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV5875612.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            19377..21065
                     /locus_tag="RJE42_000152"
     CDS             19377..21065
                     /locus_tag="RJE42_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDV5875613.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            21098..22819
                     /gene="tarS"
                     /locus_tag="RJE42_000153"
     CDS             21098..22819
                     /gene="tarS"
                     /locus_tag="RJE42_000153"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV5875614.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKVEDQPQWI
                     NALGDFIQAVPVRVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGQYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            22963..23637
                     /gene="scdA"
                     /locus_tag="RJE42_000154"
     CDS             22963..23637
                     /gene="scdA"
                     /locus_tag="RJE42_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDV5875615.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNEVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            23884..25638
                     /locus_tag="RJE42_000155"
     CDS             23884..25638
                     /locus_tag="RJE42_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDV5875616.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            25641..26381
                     /locus_tag="RJE42_000156"
     CDS             25641..26381
                     /locus_tag="RJE42_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDV5875617.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSASTIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            26494..26937
                     /gene="lrgA"
                     /locus_tag="RJE42_000157"
     CDS             26494..26937
                     /gene="lrgA"
                     /locus_tag="RJE42_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDV5875618.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            26930..27631
                     /gene="lrgB"
                     /locus_tag="RJE42_000158"
     CDS             26930..27631
                     /gene="lrgB"
                     /locus_tag="RJE42_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDV5875619.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            27972..30263
                     /locus_tag="RJE42_000159"
     CDS             27972..30263
                     /locus_tag="RJE42_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HDV5875620.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHVEIAVVKDKVMKEIRINRYSEKEDQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKIEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYEFDMKPTYESERELPK
                     IEVDSKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIV
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGNEPKKQGVLVDILFWALIIVVMGSMIGEW
                     SHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQVN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKKSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNVGLAGMVFVTLIPVGYIQLKDGLEDGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
     gene            complement(30489..31193)
                     /locus_tag="RJE42_000160"
     CDS             complement(30489..31193)
                     /locus_tag="RJE42_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5875621.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            31342..32133
                     /locus_tag="RJE42_000161"
     CDS             31342..32133
                     /locus_tag="RJE42_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV5875622.1"
                     /translation="MSNKYKDQAQDILTAVGGVENIVDVSYDTVRMTIHMHHAIPSTA
                     NEVTQIDGVASVDENETQLVIVFNEEVKYVYQQLQLLMDDAKHQEDTNHDAVDTQETE
                     QKAQAKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHKEFKVIAPFSGK
                     VSMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQEQGYNSDVIVVISNSSDFNKVELTMNEIITTEDVIFKIFKN"
     gene            32149..33585
                     /locus_tag="RJE42_000162"
     CDS             32149..33585
                     /locus_tag="RJE42_000162"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV5875623.1"
                     /translation="MTKLPQSFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVNTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            33704..33901
                     /locus_tag="RJE42_000163"
     CDS             33704..33901
                     /locus_tag="RJE42_000163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875624.1"
                     /translation="MCELAGLQHREIGTAISTDNASWRGPNTEASEKSAYNNVQVGGG
                     PNTEAGEKSAYNNVQVGAGQK"
     gene            complement(34161..34922)
                     /locus_tag="RJE42_000164"
     CDS             complement(34161..34922)
                     /locus_tag="RJE42_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV5875625.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKAATDWLIQQGAFSQDKHVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTRLRKDMNYITFVAKKEH"
     gene            34942..35076
                     /locus_tag="RJE42_000165"
                     /pseudo
     CDS             34942..35076
                     /locus_tag="RJE42_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35174..36088)
                     /gene="rbsK"
                     /locus_tag="RJE42_000166"
     CDS             complement(35174..36088)
                     /gene="rbsK"
                     /locus_tag="RJE42_000166"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV5875626.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEEKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEIPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(36116..36520)
                     /gene="rbsD"
                     /locus_tag="RJE42_000167"
     CDS             complement(36116..36520)
                     /gene="rbsD"
                     /locus_tag="RJE42_000167"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV5875627.1"
                     /translation="MKKTAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            complement(36535..37416)
                     /gene="rbsU"
                     /locus_tag="RJE42_000168"
     CDS             complement(36535..37416)
                     /gene="rbsU"
                     /locus_tag="RJE42_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDV5875628.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHRIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            complement(37648..38646)
                     /locus_tag="RJE42_000169"
     CDS             complement(37648..38646)
                     /locus_tag="RJE42_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV5875629.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     CLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVTGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            38797..38977
                     /locus_tag="RJE42_000170"
                     /pseudo
     CDS             38797..38977
                     /locus_tag="RJE42_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            39027..39419
                     /locus_tag="RJE42_000171"
     CDS             39027..39419
                     /locus_tag="RJE42_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875630.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
     gene            complement(39548..40924)
                     /locus_tag="RJE42_000172"
     CDS             complement(39548..40924)
                     /locus_tag="RJE42_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5875631.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGDHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIIIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATITFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            41158..42150
                     /locus_tag="RJE42_000173"
     CDS             41158..42150
                     /locus_tag="RJE42_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDV5875632.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSIHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPAPANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            42476..43426
                     /gene="lytM"
                     /locus_tag="RJE42_000174"
     CDS             42476..43426
                     /gene="lytM"
                     /locus_tag="RJE42_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDV5875633.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(43478..44137)
                     /locus_tag="RJE42_000175"
     CDS             complement(43478..44137)
                     /locus_tag="RJE42_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875634.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     SINDTTINEYLQQLNFNDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(44151..45071)
                     /locus_tag="RJE42_000176"
     CDS             complement(44151..45071)
                     /locus_tag="RJE42_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875635.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVTALVLIGMGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLTIFM"
     gene            complement(45068..46234)
                     /locus_tag="RJE42_000177"
     CDS             complement(45068..46234)
                     /locus_tag="RJE42_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875636.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSTVGVVWIIFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLIIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLLINLAVLVWRYRQ"
     gene            complement(46302..47825)
                     /locus_tag="RJE42_000178"
     CDS             complement(46302..47825)
                     /locus_tag="RJE42_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875637.1"
                     /translation="MSKKLKIIIPIIVVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            complement(48156..49049)
                     /locus_tag="RJE42_000179"
     CDS             complement(48156..49049)
                     /locus_tag="RJE42_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5875638.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNAPNNNHQNQSRTTDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            49297..49590
                     /gene="esxA"
                     /locus_tag="RJE42_000180"
     CDS             49297..49590
                     /gene="esxA"
                     /locus_tag="RJE42_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDV5875639.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            49673..52702
                     /gene="esaA"
                     /locus_tag="RJE42_000181"
     CDS             49673..52702
                     /gene="esaA"
                     /locus_tag="RJE42_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDV5875640.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKYITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKTTSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIVTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            52702..53160
                     /gene="essA"
                     /locus_tag="RJE42_000182"
     CDS             52702..53160
                     /gene="essA"
                     /locus_tag="RJE42_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDV5875641.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQHEEEKRINNDLNQYDTTL
                     FNKDSKEVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            53132..53374
                     /gene="esaB"
                     /locus_tag="RJE42_000183"
     CDS             53132..53374
                     /gene="esaB"
                     /locus_tag="RJE42_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDV5875642.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            53387..54721
                     /gene="essB"
                     /locus_tag="RJE42_000184"
     CDS             53387..54721
                     /gene="essB"
                     /locus_tag="RJE42_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDV5875643.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKEDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            54743..59188
                     /gene="essC"
                     /locus_tag="RJE42_000185"
     CDS             54743..59188
                     /gene="essC"
                     /locus_tag="RJE42_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDV5875644.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNVFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIVLSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVLMEDIQET
                     YDLEKIIHEEHKLPLGLDFEDVELVSLDLTLPSILTARTPNDLEIVNNRVLDGMSKLK
                     ESVTTILVDADENMSDKASIVNSYYASSEDLQLIKQGFIVEIKKRLNSERSKESVKIV
                     FINNLKAFISITGLNEKDIRYILSEGPKVNVYTIISSMYNDILGTFDRESKLARQIIN
                     QAVIVTRLYDQEFLQAKITNREPLLKPYEMYYFNKNEHIKIKLIQ"
     gene            59200..59496
                     /locus_tag="RJE42_000186"
     CDS             59200..59496
                     /locus_tag="RJE42_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDV5875645.1"
                     /translation="MGEIKVETSSTIGKIESISNAGSNVSFDNVSDSLEYTNISPFTG
                     FAAASSTLTTAISNYHSIITQDTEAMKTAVNDFRDKDADIAGQIDGNNNLGAGL"
     gene            59514..59909
                     /locus_tag="RJE42_000187"
     CDS             59514..59909
                     /locus_tag="RJE42_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HDV5875646.1"
                     /translation="MTNKEELKKRKARCIEQKHSKESELKSLQGDKKRLQDAVNSAQT
                     IIEDFNSEKKTYDGIEIEKNQWSGQTRNKSDDNRTNLNTSIDKYKEKFDKALNQMKKD
                     LENLESKIEGVETDISNIAAEIQNIDSQL"
     gene            59935..61488
                     /locus_tag="RJE42_000188"
     CDS             59935..61488
                     /locus_tag="RJE42_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5875647.1"
                     /translation="MGNKIKMSEVNNFNASLQKSLSSLSTNTKTLKNNINKLKDDSEF
                     KGKTASNINSYNQSFHIETISRIEKIKEEFEKKLKDAIEDFQNSVDNDHNAILDEDAI
                     EKYKGEVKKSIEQIESIKTRMNSTIGGVHDLTTAKTISGKDVKEKGETFNKQITDTLD
                     NFNNFQSLHFLDDSELLSMITPVSTMASKVKNMPSNRSTISGNAGYIKSEYKLHSENN
                     QFRELKKELEKYESAIYGGNKYRKTVKSMIEIKNALIVAYIAGDGNIFKGNELLAKNQ
                     LGKLGNKKLKLINATMQTNVENIEGKRLVKAAKFILDKNNKMKFSEKLKAGFKMTRNY
                     SDTEFKQLEKVAGRYNGKSAMKRGAKAAWESMSQKQLREFLKNPKNLKNICLDEVKDF
                     LGKNKLGKFLKSMRYAGKLLGPLGAVAAVANNVVNEKSGQKKIVGSIVDLGAIGGSAA
                     TGTAIGAGIGGPFGAAIGAAIGTMVGISTDIKIVGGKSLTDLAKDKLNNTVSSIRSNG
                     FGKTLSNVF"
     gene            61502..62188
                     /locus_tag="RJE42_000189"
     CDS             61502..62188
                     /locus_tag="RJE42_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDV5875648.1"
                     /translation="MNNTKENIDTLRKPGAQALSLASLFMILFSCLTFFYGLDYERFP
                     NYLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITAVFAT
                     CNLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTH
                     FGLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIMQYSRFY
                     IFELNTADDD"
     gene            62309..62758
                     /locus_tag="RJE42_000190"
     CDS             62309..62758
                     /locus_tag="RJE42_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002475708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HDV5875649.1"
                     /translation="MELDALVMPNLAKMEFSINPEYWKDEMSRIKSFFIDKRIYTVGP
                     IVFTREVVGLDEMKFVTYISLNSEIDDIPELNIKFIPSIALLPTISHKCFDEDEFENV
                     YMKIKEFAVENNITLSNSPFYHVMVDYFGGQVYEIHAEINLDEDELE"
     gene            62755..63204
                     /locus_tag="RJE42_000191"
     CDS             62755..63204
                     /locus_tag="RJE42_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HDV5875650.1"
                     /translation="MNELSYSTIMFRNVISKEYLDIDLKNLNEVTTHFLNEVKHYDLN
                     ENGPLILTINQLMKNEKVNITIYSSINNISSLHDSFEFQSYLYIDQMLHNQLRPLNYE
                     EDIKKGLEDIERFAKENQLQRISPYYFILNDVNGFKWIDIKVKVMEY"
     gene            63214..63825
                     /locus_tag="RJE42_000192"
     CDS             63214..63825
                     /locus_tag="RJE42_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDV5875651.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVVIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAFRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLIIRVRKGTFAFVVIRPILFSCWLFILNMILSRLL"
     gene            64022..64522
                     /locus_tag="RJE42_000193"
     CDS             64022..64522
                     /locus_tag="RJE42_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDV5875652.1"
                     /translation="MTFEEKLSQMYNEIANEINGMIPVDWEKVYAMAYIDDGGGEVFY
                     YYTEPGRNELYYYTSVLNKYDISESEFMDSEYELYKQFQKLRNIFKEEGHEPWTSCEF
                     DFTREGKLKVSFDYIDWINSEFGQIGRQNYYKYRKFGILPETEYEINKVKEIEQYVKE
                     QEEAEI"
     gene            64533..65024
                     /locus_tag="RJE42_000194"
     CDS             64533..65024
                     /locus_tag="RJE42_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDV5875653.1"
                     /translation="MNFEEKLSQKYNEIANKISSMIPVEWEKVYAMAYINERNGEVFY
                     NYTEPSSDELFYYTSVLNKYNIPRSEFMDSVYELYKQFDNLRELFIEEGLEPWTSCEF
                     DFTREGKLNVSFDYIDWTKLEFGQIAKENYYMYKKFGVMPEMKYEINKVKEVEKCIKE
                     QEE"
     gene            65233..>65412
                     /locus_tag="RJE42_000195"
     CDS             65233..>65412
                     /locus_tag="RJE42_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDV5875654.1"
                     /translation="MTTKENIDILRKPGAQALSLISLFLILFSCLTFFFGLDYESFPN
                     YLKITTIIELIIIVIS"
BASE COUNT        23202 a         9480 c        12138 g        20592 t
ORIGIN      
        1 gaaccccaac gattgcgtgc acttatttga ctaattcaaa aaacattgtt gttttcttag
       61 aaaaaattaa acatgataat aaaaatgtga aagtataaat aattactggt gaagtacgaa
      121 gactaaagac atctaagatg taatcgtata caaattaaaa aggtgtaaaa attaaaataa
      181 aatgtgaaat aaatcacaat ttaatattga cccggtactt aatgcatgtt acattttata
      241 tgtgaaataa atcacaaact taaaagcgga tgacacatga ccttttaagt tatgcgttga
      301 aaataaaaga gatgtttatt tgcttttgta tcgtcaataa gcagcattaa actaacatat
      361 ttgaaactac acgtatgtta gtgaattaat cataagggag tttttgtaat gaacaaattt
      421 aaagggaaca aagttgtatt aataggtaat ggtgcagtag gttcaagcta cgcattttca
      481 ttagtgaacc aaagcattgt tgatgaatta gtcatcattg atttagacac tgaaaaagtt
      541 cgaggagatg ttatggattt aaaacatgcc acaccatatt ctccaacaac agttcgtgtg
      601 aaagctggcg aatacagtga ttgtcatgat gcggatctag ttgtcatctg tgctggtgct
      661 gcacaaaaac ctggagaaac acgtttagat ttagtatcta aaaacttgaa aatattcaaa
      721 tcaattgttg gtgaagtaat ggcatcaaaa tttgatggta ttttcttggt agctacaaat
      781 cctgttgata ttttagcgta tgcaacatgg aaattctctg gtttacctaa agaacgtgtt
      841 ataggttctg gtacaatttt agactctgca cgctttagat tattgttaag cgaagcgttc
      901 gatgttgcgc cacgtagtgt cgatgctcaa attattggtg aacatggtga cactgaatta
      961 ccagtatggt cacacgctaa tattgcgggt caacctttga agacattact tgaacaacgt
     1021 cctgagggca aagcgcaaat tgaacaaatt tttgttcaaa cacgtgatgc agcatatgac
     1081 attattcaag ctaaaggtgc cacttattat ggtgttgcaa tgggattagc tagaattact
     1141 gaggcgattt tcagaaatga agatgccgta ttgactgtat cagcattatt agaaggcgaa
     1201 tatgatgaag aagatgttta tattggtgtt ccagcagtca tcaatagaaa cggtattcgc
     1261 aacgtcgtag aaatcccatt aaacgacgaa gaacaaagca agttcgcaca ttcagctaaa
     1321 acattaaaag atattatggc tgaagcagaa gaacttaaat aactttttat aaaatctata
     1381 ccattccaaa aattgtaaaa ccttacccca aaaaattgta taaagggcta tttgatacaa
     1441 ctatacgtgt cgagtagccc tatttttaat gtagtgaaag tcgttgttga aattaaattt
     1501 aaatctggca cttaagcttt aatcatagca ttaagatgat tgtctagcag aggcgatttg
     1561 cggactcact acagtgcatg atgaacttaa tgcttcaaat gtaacattaa aaataaaagc
     1621 aacgatgtca cttcttactt cgtacatcgt tgccattagt tctgttgata tttcggttag
     1681 tcttaatccc cgagcaattc ttcaatttca tttttgataa ctgtaacgtg aggcccataa
     1741 attacttgca caccagtgcc ttgctggatt acacctttgg caccagtact ttcgagtaat
     1801 actttatcga ctttgtcatt ttgatgaagt gtgacgcgta gtctcgttgc acaacagtca
     1861 acgatttcaa tgttatcttc gcctcccaaa ccagcaacaa tagtttgtgc tctttcagta
     1921 gcctcaactt gttgtgctgc agctttatct tcacgaccag gtgttttgaa attaaatttc
     1981 gtaattaaga atctgaaaac gatgtaatac aaacagagcc acacaattcc aataggtatg
     2041 acgtataggt agtttgtttt actattacct tgtagcacac caaaaagtaa gaaatcgata
     2101 aagcctccac tgaaggtttg accaattgta atgttgaaaa tgtctgacat cataaatgct
     2161 aatccatcaa agaaggcatg gattacataa agaataggtg cgacaaacaa gaaactaaac
     2221 tctaaaggtt cggtaatgcc tgttaaaaat gaagtgagtg cagcggataa cattaaaccg
     2281 ccgacaactt ttttatgttc aggtttagct gtgtgataaa ttgcaagtgc ggcaccacat
     2341 aagccgaaca tcatcgtaat aaaacggcct gacataaagc gtgacacacc tgaataatac
     2401 ttcgtcacat ctggatcacc aagttgagca aagaagatgt tctgcgtacc ttgaactaag
     2461 tgccctttga cttctaaagt accaccaagt gccgtctgcc aaaacggtaa gtaaaaaata
     2521 tgatgtaaac cgagtggacc taacaatctt aagatgaagc cataaacaaa agtaccgatg
     2581 gcacctgttt tcgttacaaa tccaccaaca tgataaatgc cggcttgtat gcttggccaa
     2641 atgaaataca tcaatacacc taaaaagatt gcggcaaatg ctgtgacaat agggacaaat
     2701 ctagagccac caaagaaacc taaatacggt ggtaatacca ctttgtgata tttgttgtga
     2761 agtattgcgg tcataatacc tgtgataatc ccgccaaaaa caccagtttc aaccgtttgt
     2821 atgccgagca ccatgccttg tccattttgt gcaagctgat cttttgctaa tgtgcccgtg
     2881 atagttaata agccattcat agttgcgttc ataattaaga aaccgagcag tgcagctaaa
     2941 cctgcagtac ctttatcgct tctagataat ccgattgcga caccaattgc aaagatgact
     3001 ggtaaatttt ggaaaacaat actacctgca gctgacatta atgtaaaaat attttgtaat
     3061 aaggtaatat ctaaaatagg gtatgcttta atggtgtttg gattacttaa tgcaccaccg
     3121 atacccaaca atagacctgc agctggtaag attgcgatag gtaacataaa ggacttgccg
     3181 aactgctgtg ctttttcaaa taaagatttc atcaacatcc ctcctaatta ttctcaatat
     3241 agcttttgag aaatttaata tcaatatata ttctgtgtat gaaaatattt ttcataaaaa
     3301 ttgtttgaat catgtaacaa tcatataaat tgctgtttat attgttgtga aaatgagttg
     3361 acaaaagtcg gtatagatat gtagacatcc tatttttagc gaggtaggtt gacaaggcat
     3421 atcggataaa tttataagcc ataaaatagg taaatagtgt ttgtatccaa aaatatgaga
     3481 aagttaaaac tatttttcaa aataaacatg attcaccaca taaataaata tacataagct
     3541 atcgataaca acttaagaaa ggtggatata taaatgaaaa gaaagattat tatggattgt
     3601 gatccaggac acgatgatgc aatagcatta attttagcgg gggcaattga cagtccacta
     3661 gagatattag ctgtaaccac agtcgcaggt aatcaatcag ttgacaagaa tacgacaaac
     3721 gccttgaacg tattggatat tatgggacgc caagatatag cagtagcgaa aggtgcggat
     3781 aggccgttaa ttaaaccagc tgcctttgct tctgaaatac atggggaatc tggattagat
     3841 ggtccgaaac taccgtcgac accatcacgt caagcagttg caatgccagc atcagatgtg
     3901 attataaaca aagtgatgac gagtgataca cctgtaacaa ttgtagcgac aggtcctctt
     3961 acgaatgtag caacggcatt gattcgtgag ccaagaatcg ctgagcatat tgaatctatt
     4021 actttgatgg gtggtggtac atttggaaat tggacgccta cagcagaatt caatatttgg
     4081 gtagatgctg aagcagcgaa gcgtgttttt gaaagtggga ttactataaa tgtgtttggt
     4141 ttagatgtaa cacatcaagt tttagccgac gaacacgtga ttgaacgctt tgaaagtatc
     4201 aataatcctg ttgcacagtt cgtcgtagaa ttattgcaat tctttaagaa gacatacaag
     4261 actcacttta atatggacgg tggtccaata catgatgctt gtacaatttt gtatttgtta
     4321 caaccagaat tgtttacaat ggtacccgtt aatatcgaca ttgaacatca aagtccacta
     4381 acttatggca ctatggctgt agatttaaat catgttacag gtaagcctgc caatgcctat
     4441 tttgctacag cagttgatgt tgaagaagtg tggaacttga tagatcataa gttacgtaca
     4501 tacgaataat aatacttaat taaatagata cagttaaccc taaggtgcct gatttaagcg
     4561 tccttagggt ttttgtattg gattacaatg gtcatggcaa tttgataatg attgagatat
     4621 gtatgttaaa aatacttcaa aaaacacaaa atacaaaaat atatgcaaaa atttcaattg
     4681 ctttatttta aaagattaaa attacaaaaa ataccgatat aattggagtg ttgtcgaaac
     4741 atcacaatga tgtacatact cattttgaaa atatgtgttc aacgagtgta catcggtgtg
     4801 acgattgatg ataaattgag ctcagatgct caaaggagga tcgaacatga acggggataa
     4861 tcagcaaata ctcagagaaa ttgtattgaa tcctactatt catggtaaag aacttgaatc
     4921 gatatttggc ttatctcgta gacaactagg atatcgcatt caaaaaatca atttgtggct
     4981 tgaacaagag ggttatccaa aacttgaaag aacaagccaa ggaaatttta ttgtaagttc
     5041 tgaaatcatg acgttattca aacgagatgt atcggagcag caaatgttaa acggcaacaa
     5101 tgtcattttt agcatagaaa cacgtcgtta ttatttaatg ctcatgcttt ttagtaagga
     5161 aaacgcaatg tctctaaacc atttttcaat tgatttacaa gtcagtaaaa atactgtcat
     5221 tcacgatata aatcatgtga aagagcaatt ggaagatcat ggtttgtcac ttaagtattc
     5281 tcgaaaacat ggttatgaaa ttgttggtga tgaatttgaa gttcgccgtt tcttcattaa
     5341 gttgattgat caaaggttga atcatgatat tactaaaagt gaagttttaa aggcgctcaa
     5401 cttaacattc gaagatatcg catatcaaaa agacaagatt aaacaggtag aacaattttt
     5461 gaagagtcgc tttatagaca aatcactgag ttcattgcct tacgtccctt gtgtgattcg
     5521 tagacgaatt caaagtggtc atgtgattaa tccattaaat attaattatc agtatttgag
     5581 ggatacgaaa gaatatcaag caacggagat tatgacgcaa catgagccgg atttgccaga
     5641 agcggaaaag ttatatttga cattacactt actttcaaca agtgtgcaat ggactgattt
     5701 gcaggaatca gataacatat cgaatttaac aacggccatt gatcaaatga ttcaccattt
     5761 tgaacaaatt acttttatta acattgaaga taaggagaaa ttatcacagc aactcttgtt
     5821 acatttaacg cctgcttttt accggattaa atataaccta acggatcgtg atgaattaat
     5881 aaatccttta caaggaaatt atcaatcctt atttcatatg gtgaaacaat catgtcaatc
     5941 gttaactgaa tatttcggaa aatcattacc tgataatgaa atagcatatt taaccatgtt
     6001 gttcggaggt agtttgagac gtcaagagga gaactttgat ggcaagatta aagctattat
     6061 cgtgtgtaca caaggtacat cagtatcgca aatgatgcta tatgagttgc gaaacttatt
     6121 tccagaaatt attttcttag atgcgatttc acttagaaca tttgaaaatt acacattaga
     6181 ttatgacatc gtcttttcac caatgtttgt cctaacacat aaaaaattat ttatcacaaa
     6241 agtagcttta tctgaaaatg agcaacgaaa gttacgtaaa gatgtgatga agtacattaa
     6301 taaggaatcg gctgacattg ataaggaaat aaacaagtta atggcattaa ttgaacgcac
     6361 tacgacagtt aatgacatta cagaactacg tgatggttta gaagatttta ttgcgaatta
     6421 taattcaatt tcaaccatta atggatcgat tgtcacacaa aataagacat tagatttagc
     6481 tgacttgata ccagcaaggc acgtgaaaag aatacatcat gttgaaaata ttgatgaagc
     6541 tattgctaaa gcaagtgatg tgttagttgc taatcatttt attgatatta aatatattca
     6601 tgagatgcaa caggcatttg atgattcgta tatggttatc atgcaaaata ttgctattcc
     6661 acatgcatac tctgaaaagc atgtacataa aacggcgatg agtatgttga tattacaaga
     6721 accaatatac atgtcagatg gcacagcaat ccatattatt gtacctattg ctgctgttga
     6781 taaagtgaca cacttaagag cgttactaca attgagagat gtggcgcaag acaatgacgc
     6841 aattaagcgc atcatacaaa gtcgcaaaaa ttctgatgta aatgagattt taaaaaatta
     6901 ttcaaataaa gaagcgaggg aaaatggatg ggacagcaat tagtgcataa agaaaatata
     6961 atgctcaatt tgtcggcaac tgataaagaa tccgtattgt cacaaatgtc agatgtgtta
     7021 tttcaaaatg ggttcgtaaa gtcaacgttt aaagatgcag tcatcgacag agaaaaagag
     7081 tttgctactg gtttaccaac gcatctatgt tcggttgcta taccgcatac agatgtcgaa
     7141 catattaacc atagaacgat aggtgtggct gttctagaaa aagaagtgcc gtttattgaa
     7201 atgggaacac ttgatcaaca gacagaagtg aaaatcgttt ttatgttagc aatggataaa
     7261 ttagatgatc aacttaagct attacaacag ttgatgcaaa tttttcaaag tgaagaaaaa
     7321 ttggagcaga ttctgcgaac gaaagatgaa acgattttag caacactaat caatgattat
     7381 ttggaatata actaaaaatt aattggagga attgaaaatg aaacaagtat tagtagcgtg
     7441 tggtgcaggt attgcaacgt caacagtagt aaataatgca attgaggaaa tggcaaagga
     7501 acacaatatt aaagtagata ttaaacaaat caaaattaca gaagttggac cttatgaaga
     7561 cactgcagat ttattagtta caactgcaat gacaaagaaa gaatataaat tcccagttat
     7621 caacgcacgt aatttcttaa ctggtattgg tattgaagaa acaaaacaac aaatcttaac
     7681 agagttacaa aaataactga attgatatgt aacgtgggtc aataacatat aaatatatgc
     7741 aaattgcata taaatgacta tcacgacaaa tgattgttga tgacatagaa acgcagtgac
     7801 tgtaattcaa agggattgca gtcgaatgta gcaagggttg tcgttgaaat gactgtaacg
     7861 tcaaatgtaa tcacaatcgg aaagtatcgt gacaatgcat atataacagg gagggtttaa
     7921 atatgagtta cttcactgat tttgtaaggg gatttttaga tttaggtgca actgttattt
     7981 taccggttgt catattcttg cttggcctat tctttaggca gaaaattggg gcggcattta
     8041 ggtctggttt aacaataggt gtggcttttg tagggatttt cttagtcatc gatttattag
     8101 ttaaaaattt agggccagca gcacaagcga tggttaaaaa tttaggcgtc agtctgaatg
     8161 tgattgatgt aggttggcca gcaacatcat ctatcgcttg ggcatcatct gtcgcagcat
     8221 ttattattcc actcggaatc atagttaacg ttgtattgct agtaactaaa gtgacaaaga
     8281 cgatgaatgt agatatttgg aatttttggc attatacgtt tacagcagca atggtttatg
     8341 ccgtatcagg tagtatttgg caagcgttat tagcagcagt tattttccaa gttatctgtt
     8401 tgaaagtagc agattggaca gcaccgatga tgagtgagtt ctttgattta ccaggtgtat
     8461 cgattgctac aggaagtaca atttcttatg caccaggtat ttacttagtt aaattgttgc
     8521 aaagagtacc cggtctgaat aagttagatg ctgatcctga aacaattcaa aaacgttttg
     8581 gcgcatttgg agagtctatc tttgtcggat taattttagg tttaggtatt ggtgtgttag
     8641 caggttacaa acctggagac atcattaatt taggaatgtc aatggctgca gtaatggtat
     8701 taatgcctag aatggtaaaa atcttaatgg aaggtttaat gccagtttca gagtctgcaa
     8761 gaacatggct aaataaacgt tttggcgaac gtgaaattta tattggattg gatgcggctg
     8821 tagcattagg tcatccagcg gttatttcga cagcattaat tttagtacct atcactgttt
     8881 tattagccgt tattttacca ggaaaccaag tactaccttt tggtgactta gcaactatac
     8941 catttgttgt cgcgtttatt gttggtgcag caagaggaaa cattattcat tctgtcattg
     9001 tgggtacgat tatgattgca atttcactat atattgcaac agacgtagca cctattttca
     9061 cagatatggc gaaaggtacg aatgtacaaa tgccaaaagg ttcatctgaa atttcaagta
     9121 ttgatcaagg gggtaatatc gttaactatc ttatctttaa actatttagt ctattcaatt
     9181 aaaaatcgag gtgtttgtgg tgaaagcttt agtaaaaaca agagaaggac atggcaactt
     9241 agaacttctt gacaaagaag ttgcaacacc gctagatgat aaagtaaaga ttaaagtaca
     9301 ttatgcagga atttgtggca cagatattca tacttatgaa ggtcattata aagttaattt
     9361 tccagtgaca ttaggtcatg aattttctgg tgaaatcgtt gaagttggag cagacgttaa
     9421 agattttaaa gttggtgacc gtgtcacttc tgaaacgaca ttctatgttt gtaatgagtg
     9481 tgaatactgt gaatcaaaag actataattt atgcaaccat cgaaaaggta ttggaacaca
     9541 agttgatggc gcatttacta attatgtcat tgcacgtgaa gaaagtttgc atcatattcc
     9601 agacgaagta tcgtatcagt ctgcagctat gacagaacca ttagcatgtg cacatcatgg
     9661 cgtttctaag attcaagtca attcaggcga tgtagcagtt gtaatgggac ctgggccaat
     9721 cggattactt gtagcacaag tgttgaaaag taaaggtgca actgttgtgg taactggatt
     9781 ggacaatgac aaagtcagat tagataaagc agaagcattg cacatggatt atgtagtcaa
     9841 tttacaacaa acagacttaa aaacgtatat caatggaatt acagacggtt acggtgcaga
     9901 tgttgttgtt gaatgttcag gtgcagttcc agcagcacga caaggtttgg atattttacg
     9961 caaaaaaggt ttctacagtc aaataggtat ttttaaggat gctgaaattc catttgatat
    10021 ggaaaaagtg attcaaaaag aaataacagt tgttggtagt agaagtcaaa agccagcaga
    10081 ttgggaacct tcattgcaac ttatggcgga tggtttagta aatgctgaag ctttggtgac
    10141 aaaaatatat gatatttcga aatgggacga ggcgtatcaa catttaaaat ctggcgaagg
    10201 tattaaagca ttacttaagc cgctcgattt agatgaaaat gaaggagaga attaatatgg
    10261 tagaatcaat gctaactttt atgcttgggc cattgagaca aatcactgat ttttatatgg
    10321 aacatctact tgtaagtaat tctatcgtca ttgcaggtta ttttgcgaca ggtattttta
    10381 aaaagaaaaa agttgtgaat taaatcaaag ttgaggtgat ttacaagtga aagctttgaa
    10441 attatatggc gtggaagatt tacggtatga ggataatgaa aagccagtca ttgaaagtac
    10501 gaatgacgtt attgttaaag tacgagcgac tggaatatgt ggttcagaca cgtcacgata
    10561 caaaaaaatg gggccataca ttcaaggtat gccatttggt catgaatttt caggtgttgt
    10621 agatgccatt ggaagtgatg ttacgcatgt taatgtaggc gacaaagtga caggttgccc
    10681 agcaatacct tgttatcaat gcgagtattg tttaaagggt gaatatgcac gatgtgaaaa
    10741 gttatttgtc attggctcat atgaacctgg atcgttcgcg gaatatgtca aattgccagc
    10801 gcaaaatgtt ttaaaggttc cagacaacgt tgattacatt gaagcagcaa tggttgaacc
    10861 atcagccgtt gttgcgcatg ggttttataa atcgaatata caacctggta tgactgttgc
    10921 agtaatgggg tgtggcagta taggtttgtt agctattcaa tgggcacgaa tatttggtgc
    10981 tgcacatatc atcgctatag atatagatgc acataaacta gatattgcta catcattggg
    11041 cgcacatcaa acaatcaatt caaaagaaga aaatcttgag aaattcatcg aaaatcatta
    11101 cgccaatcaa atcgatttag ctatagaatc atcaggtgct aaagttacga ttggtcaaat
    11161 attgacgcta cctaaaaaag gtggcgaggt ggtattactc ggaataccat atgatgatat
    11221 tgagattgat cgcgttcatt ttgaaaaaat tctgcgtaac gagttgacag tatgtggctc
    11281 ttggaactgt ttgtccagta attttccggg gaaagagtgg acggcaactt tacattatat
    11341 gaagacgaaa gatattaatg taaagcctat tatttctcat tttttaccgt tagaaaaagg
    11401 cccggagaca tttgataaat tagttaacaa gaaagaacga tttgataaag tcatgtttac
    11461 gatttattag tatgcacctt tgaggacgaa aaggctggta tagttatagc tatgaaagtg
    11521 cgaatgccgt ctggtctaca gatactatcg aaataattca tcatcgaata tacgttgcta
    11581 aatagccggt ttacttgtgt gaaatatgct tgtgaatcgg ttgttttgca ttttgtatac
    11641 ttaaaatgag atggcaatat ttgataattt ttagagtgaa aatcaagtac agccacttaa
    11701 taagataaat ttattataat atatggtaaa atgatggcag taataatgaa tttgaaaaag
    11761 agtaaacatt aataccttta acaatttaat atcgtcagtg ttaatgatta actgcatggc
    11821 aaaacaactt agaatggtca gttacaaaaa tacattttta taaaaaatta tcacactatt
    11881 gtgacaactg cctttaactt aataaaagag gcaaatgagc aataggttag gcttatgtgc
    11941 gggcataggt cagtaatgta taaatggaaa tgatgtaatg acagaatgga ggacaacatg
    12001 atttatgcag gtattttagc aggaggtatt ggttcgagaa tgggaaacgt gccattacca
    12061 aaacaatttt tagatattga taataaaccg attttaattc atacaattga gaagttcatt
    12121 ttagtgagtg aatttaatga gattattatc gcaacgccag cacagtggat ttcgcatacg
    12181 caagatattt taaaaaaata taatattaca gatcagcgcg ttaaagtagt tgcaggtggt
    12241 acggatcgaa acgaaacaat catgaacatt atcgaccaca ttcgcaatac gcagggtatc
    12301 aacgatgatg atgtgattgt aactcatgat gccgtaagac catttttaac tcaacgtatt
    12361 ataaaagaga acattgaagt agcagcaaaa tatggtgcag tagatacagt cattgaagca
    12421 attgatacga ttgtaatgtc taaagataaa cagaacatac acagtatccc tgtaaggaat
    12481 gaaatgtatc aaggccaaac accacaatca tttaatatta aattattaca agatagttat
    12541 cgcgccttaa gtagtgcaca aaaagaaatc ttatcagatg catgtaaaat cattgtcgaa
    12601 tctggacatg cagttaaatt ggtacgtgga gaactataca acattaaagt gacaacaccg
    12661 tatgatttaa aagtagcaaa tgccattatt caaggtgata ttgccgatga ttaatcaagt
    12721 atatcaactc gttgcaccga gacagttcga cgtcacatat aataatgttg atatttatgg
    12781 taatcatgtc atcgtaagac ctttatactt gtctatttgt gcagctgatc aaaggtatta
    12841 cacaggtcga agagatgaaa atgtactgcg taaaaaatta ccaatgtcac tagttcatga
    12901 agctgttggt gaagttgtat tcgatagtaa aggcgtattt gaaaaaggaa cgaaagtagt
    12961 aatggtgccg aatacaccta cagaaaaaca cgacgtgatt gccgagaatt atctgccttc
    13021 tagttatttt agatctagtg gttatgatgg ttttatgcaa gactacgttg tgatggcaca
    13081 tgatcgtatc gttccgctgc ctaatgacat tgatttgagt acgatttcat acacagagtt
    13141 agtgtcagta agttatcatg ctatacaacg atttgaacgt aaatctatac ctttgaaaac
    13201 cagttttggt atttggggtg atggtaactt aggttatatt acagctattt tgctacgtaa
    13261 gttgtaccca gaagctaaaa tttatgtatt tggtaagaca gactataaat taagtcattt
    13321 ttcatttgta gatgacatct ttacagtaaa tcaaatacca gatgatctta aaattgatca
    13381 tgcatttgaa tgtgttggag gtaaaggaag tcaagttgca cttcaacaaa tagttgaaca
    13441 tatttcacca gaaggcagta ttgctttgtt aggcgtaagt gaattacccg tggaagtgaa
    13501 tacacgatta gtacttgaaa aaggattaac gttgattggt agtagtcgaa gcggctctaa
    13561 agattttgag caagttgttg atttatatcg taagtaccca gacattgttg aaaagttagc
    13621 cttattaaaa ggacatgaaa ttaatgtatg tacgatgcaa gatatcgtcc aagcgtttga
    13681 aatggattta tcgacatctt ggggaaaaac agtattgaaa tggacgattt aataaaccga
    13741 tgggggaata acaatgacaa aaacgaaaca agcaatacat attgataaca tatactggga
    13801 acgtgttcag ttatatattg aaggacatag tgaaggtgtc gatttaacat caggacaatt
    13861 tgttctgagg aatttaaccg aaacaaaaac attagaagca aatgaaatga aaatagacgg
    13921 taatacattt aaatgtagat tcaacgtcgc aatattagac gatgggtatt atttaccaat
    13981 ggataaatat ttatttgttt atcatgacca gttagagtat attggacaac ttaatccaaa
    14041 tattattgat caagcttatg cggcattaaa tgaagagcaa attgaagaat acaatgagct
    14101 gactacacaa aatggaaaag caaactatgt gttagccaac gaagctaaag ttttccgcaa
    14161 aggtggcgta tcacaacata cggtttatac cattactcca gaaatagcaa gtgacgttaa
    14221 cgaatttgta tttgatattg aaatcacctt acctcaagag aaatcagggg tcattgcgac
    14281 aagcgcacac tggcttcata aacaaggcca taaagcttcg tttgaaagta gaagtttctt
    14341 atttaaagct atttttaata ttacaaagtt actacatatt aaaagaagca aaacaatatt
    14401 attcacatca gattcgcgtc cgaatttatc agggaatttc aagtatgtat atgatgagtt
    14461 actacgccaa aaagtagatt ttgattatga tattaaaacg gtatttaagg cgaatattac
    14521 ggatagacgt aaatggagag acaagtttag attgccatat ttacttggta aggctgatta
    14581 tatttttgtc gatgatttcc atccattaat ttatacggtt cgctttagac catcacaaga
    14641 aattattcaa gtgtggcatg ccgttggtgc ctttaaaaca gttggcttta gtcgtacagg
    14701 taaaaaaggt ggtccgttta tcgattcgtt aaatcatcgt agttacacga aagcatatgt
    14761 atcatcagaa accgatattc cattttatgc tgaagcattt ggaattagag aagaaaatgt
    14821 tgtaccaaca ggtgtaccac gtactgatgt actatttgat gaagcttatg caacacaaat
    14881 taaacaagag atggaagatg aattgccaat tataaaaggt aagaaagtta ttctattcgc
    14941 accgacattt agaggtaatg gtcacggtac ggcacattat ccatttttta aaattgattt
    15001 cgaacgttta gcaagatact gtgaaaaaaa taatgcggtt gtattattta aaatgcatcc
    15061 atttgtaaaa aatagactca atatttcacg tgaacataga caatacttta tcgatgtatc
    15121 agattatcgt gaagtcaatg atattctctt tgttacagac ttattgatta gtgattattc
    15181 atcattaata tatgaatacg cagtatttaa aaagccaatg attttctatg catttgactt
    15241 agaagattat attacgacgc gtgatttcta tgaaccatat gaatcatttg ttccaggtaa
    15301 gattgtgcaa tcatttgacg cattaatgga tgctttggac aatgaagatt atgaggttga
    15361 aaaagttgtg ccattcttag ataaacattt taaatatcaa gatggtcgct caagtgaacg
    15421 tttagtcaaa gatttgttta gacgctaatg ttggcatata ttacttgcca tgcaaatgag
    15481 tcataaggca tagttttcaa gaagggttct gacaatgaag tgagcaacat gtcatgatga
    15541 gaagcaggac tatacaatga gaataacctt ttgatttttc atgcataagg gagtgggaca
    15601 gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc caacttgcac attattgtaa
    15661 gctgactttc cgccagcttc tatgttgggg cccacacccc aacttgcatt gcctgtagaa
    15721 tttcttttcg aaattctctt tgttggggcc cacaccccaa cttgcattgc ctgtagaatt
    15781 tcttttcgaa attctctttg ttggggcccc tgactagaat tgaaaaaagc ttgttacaag
    15841 cgcattttcg ttcagtcaac tactgccaat ataacttcgt agagcataga acattgattt
    15901 atgtcccagc ctgttccaac tgaattgtca ttttgtaaaa taaaataatc aatccaatcg
    15961 ttatctaatt cataaatgtg taaacacata cgttatgaat ggataacgat tttttgttat
    16021 gttaaagtgg tacattaatc atgtatttcg tatgataatt aacgacaagt gtaatggtta
    16081 aatgcatttt atgatgaaat gctataatag gcatggttac aatgagcttg ctcatacata
    16141 ttaatataat tacaaaaaca cgtcggaggt acgacatgat taaaactaca attaaaaaat
    16201 tgatagaaca cagtatatat acgactttta aattactatc aaaattgcca aacaagaatc
    16261 taatttattt tgaaagcttt catggtaaac aatacagcga caatcccaaa gcattatatg
    16321 aatacttaac tgaacatagc gatgcccaat taatatgggg tgtgaaaaaa ggatatgaac
    16381 acatattcca acagcacaat gtaccatatg ttacaaagtt ttcaatgaaa tggtttttag
    16441 cgatgccaag agcgaaagcg tggatgatta acacacgtac accagattgg ttatataaat
    16501 caccgcgaac gacgtactta caaacatggc atggcacgcc attgaaaaag attggtttgg
    16561 atattagtaa cgttaaaatg ctaggaacaa atactcaaaa ttaccaagat ggctttaaaa
    16621 aagaaagcca acgttgggat tatctagtgt cacctaattc atattcgaca tcgatatttc
    16681 aacatgcatt tcatgttagt cgaaataaaa ttttggaaac aggttatcca agaaatgata
    16741 aattatcaca taaacgcaat gatactgaat atattaacgg tattaagaca agattaaata
    16801 ttccattaga taaaaaagtg attatgtatg cgccaacttg gcgtgacgat gaagcgattc
    16861 gagaaggttc atatcaattt aatgttaact ttgatataga agctttgcgt caagcgctgg
    16921 atgatgatta tgtcatttta ttacgcatgc attatttagt tgtgacacgt attgatgaac
    16981 atgatgattt tgtgaaagac gtttcagatt atgaagacat ttcggattta tacttaatca
    17041 gcgatgcgtt agttaccgac tactcatctg tcatgttcga cttcggtgta ttaaagcgtc
    17101 cgcaaatttt ctatgcgtat gacttagata aatatggcga tgagcttaga ggtttttaca
    17161 tggattataa aaaagagttg ccaggtccaa ttgttgaaaa tcaaacagca ctcattgatg
    17221 cattaaaaca aatcgaagag actgcaaatg agtatattga agcacgaacg gtattttatc
    17281 aaaaattctg ttcattagaa gatggacacg tgtcacaacg aatttgccaa acgattttta
    17341 agtgataact taaaaacaat aaaaaattat aaattaatta gttaagtgat ataaataata
    17401 aacgaaatgt ttgcttgtat gttattattt gtgtatgaaa acgtctgata taatgtaata
    17461 taggtttaaa caaaatatgc taaaatataa gcaaaatatg tataatttta agtaatacac
    17521 agacaattaa agttttgatt ttaaaatgag ttttcaattg aaaaatgcaa cgaaatttaa
    17581 ataattaaat atagatatag ttgaatggag gaagtatttt atgaaatacg ctggtattct
    17641 agctggaggt ataggctcaa gaatgggtaa cgtaccttta cctaaacaat ttttagattt
    17701 agacaacaaa ccgattttaa tccatacatt agaaaaattt attttaatta atgattttga
    17761 aaaaattatt atcgcgacgc cacaacaatg gatgacgcat acgaaagata cacttagaaa
    17821 attcaaaatt tctgatgaaa gaattgaagt cattcaaggt ggtagtgatc gtaacgatac
    17881 aattatgaat atcgttaaac atattgaatc aacaaatggt attaacgatg acgatgtcat
    17941 tgtgacacat gatgcagtta gaccattttt aacgcatcgt attattaaag aaaatattca
    18001 agctgcttta gagtacggtg cagtagatac agtgattgat gctatagata cgattgttac
    18061 atctaaagat aatcaaacga ttgatgcaat tccagtgcgt aatgaaatgt accaaggtca
    18121 aacacctcaa tcgtttaata ttaatttatt aaaagaaagt tatgcacagt tgagtgatga
    18181 gcaaaagagt attttatctg atgcttgtaa gattattgta gaaacaaaca aaccggttcg
    18241 acttgtaaaa ggtgagttat ataacattaa agtaacaaca ccttacgatt taaaagtagc
    18301 gaatgctatt attcgaggtg gtattgccga tgattaatca agtatatcaa ttagttgcac
    18361 ctagacaatt tgaagttacg tataacaacg tagatattta cagtgactat gtcattgtac
    18421 gtcctttata tatgtcaatt tgtgctgccg atcaaagata ttatactggt agccgtgatg
    18481 agaatgtctt atctcagaaa ttgccaatgt ctttaattca tgaaggtgtt ggtgaggtcg
    18541 tatttgacag taaaggtgtg tttaataaag gtacaaaagt agttatggta ccgaatacgc
    18601 cgacagaaaa agacgatgtc attgctgaaa actatttaaa atcaagttac ttcagatcaa
    18661 gtggacatga tgggtttatg caagattttg tgttgctaaa tcatgataga gctgtaccac
    18721 tacctgatga tattgattta agtattattt catatacaga gcttgtaaca gtaagtttgc
    18781 atgctattcg tcgttttgaa aagaaatcta tttcaaataa aaatacattt ggtatttggg
    18841 gtgatggtaa cttaggttac attacagcca ttttattacg taaattatat ccagagtcta
    18901 aaatatatgt ctttggtaaa acagattata aattgagtca cttctcattt gttgatgatg
    18961 tcttctttat taataaaata cctgaaggct taacatttga tcatgcattt gagtgcgttg
    19021 gtgggcgtgg tagccaatca gcaataaatc aaatgattga ttatatttca ccagaaggaa
    19081 gcattgcact gttaggtgta agtgagttcc cagtagaagt taatacacgt ctagtattgg
    19141 aaaaaggact aacgttgatt ggtagtagtc gaagtggttc aaaagatttc caagatgttg
    19201 tagacttata cattcaatac ccagatattg tagataaatt agcgttgtta aaaggtcaag
    19261 aatttgaaat tgcaacaatt aatgatctta cagaagcttt tgaagcagac ctgtctacat
    19321 cttggggtaa aacagtatta aaatggatta tgtaaataga aaaggatgaa ttaacgttgg
    19381 ttaaaagtaa gatatatata gataaaatct attgggaacg tgttcagtta ttcgttgaag
    19441 gacatagtga aaacctagat ttagaagata gtaattttgt attaagaaat ttaactgaga
    19501 cacgtacaat gaaggcgaat gatgtcaaaa tagatgggaa tcaattcgtt tgtcgtttca
    19561 atgtagctat cttagataat ggttattact tacctgaaga taagtactta ttagtgaatg
    19621 agcaagaact tgattatatt gcacagttaa acccagatgt gattaatgat gcatatcaaa
    19681 atctaaagcc agaacaagaa gaagaataca acgaattaga aacacaaaat ggtaaaatca
    19741 atttcttatt gcagacttac ctaaaagaat ttagaaaagg cggcatttca aagaaaacgg
    19801 tttatactgt tacacctgaa atttctagcg atgttaatga atttgtcctt gatgttgttg
    19861 taacgactcc ggaagttaaa agtatttata tcgttcgtaa atataaagaa ttacgaaagt
    19921 atttccgcaa acaatcattt aatacaagac aatttatttt taaagcgata tttaatacga
    19981 cgaaattttt ccacttgaaa aaagggaata cggtgttgtt cacatcagac tctagaccaa
    20041 cgatgtctgg aaactttgaa tacatctata acgaaatgtt acgtcaaaat ttagataaaa
    20101 agtatgatat tcacactgtt tttaaagcga atattacaga tagacgtggc atcatcgaca
    20161 agtttagatt gccatattta cttgggaagg cagactacat ctttgttgat gactttcacc
    20221 cattgattta tacagtgcgt tttagacgtt ctcaagaagt tattcaagtg tggcatgccg
    20281 ttggtgcctt taaaacagtt ggctttagtc gtactggtaa aaagggcgga ccatttattg
    20341 attcattaaa tcatcgtagc tatacaaaag cttatgtatc atctgaaacc gatattccat
    20401 tctacgctga agcatttggt attaaagaga aaaatgtagt gcctacaggt gttccacgta
    20461 ctgatgtact atttgatgaa gcttatgcga cacagatcaa acaagagatg gaagatgaat
    20521 tgccaattat taaaggtaag aaagtcattc ttttcgcacc aacatttaga ggtagtggtc
    20581 atggtacagc acattatcca tttttcaaaa ttgatttcga acgtttagca agatattgcg
    20641 aaaaaaataa cgcggttgta ttatttaaaa tgcatccatt tgtgaaaaat agacttaata
    20701 ttgcagacaa acataaacaa tattttgttg acgtttctga ctttagagaa gttaatgata
    20761 tactgttcat aacagattta ttaattagtg actattcatc tttaatatat gaatatgcag
    20821 tatttaaaaa gccaatgatt ttctatgcat ttgatttaga agattatatt acgacgcgtg
    20881 atttttatga gccatatgaa tcatttgttc caggtaaaat tgtgcaatca tttgacgcat
    20941 taatggacgc cttggacaat gaagattatg aaggagaaaa agtcattcca ttcttagata
    21001 aacattttaa atatcaagat ggccgatcaa gtgagcgttt agtcagaaat ttatttggta
    21061 gctaagttta tatagtagtc aaagtgggag aggtataatg atgaaatttt cagtaatagt
    21121 tccaacatac aattcagaaa agtatataac agaattactc aatagccttg cgaaacaaga
    21181 ttttccgaaa actgaatttg aagtggttgt agttgatgac tgttcaacag atcaaacgtt
    21241 acaaatagtt gaaaagtatc gtaataaatt gaacttgaaa gtaagtcaac tcgaaacaaa
    21301 ttctggtggt ccaggtaaac ctagaaatgt ggcgttaaaa caagcagaag gtgaatttgt
    21361 attatttgtg gactccgatg actatataaa caaagagact ttaaaggatg cagcagcatt
    21421 tattgatgaa catcactcag atgtcttatt gattaaaatg aaaggtgtta atggtcgtgg
    21481 tgtaccacaa tctatgttta aagaaacagc acctgaagtt actttgttga attcaagaat
    21541 tatctatact ttaagcccga ctaaaatcta tagaacagca ttactaaaag ataatgacat
    21601 ttattttcca gaagaattaa agagtgcaga agatcaatta tttacaatga aagcatattt
    21661 gaatgcaaat cgaatcagtg tgttaagtga taaagcgtat tattatgcta caaagcgtga
    21721 aggggaacat atgagtagtg cgtatgtttc acctgaagac ttttacgaag tcatgagatt
    21781 gattgctgta gaaatattaa atgcagattt agaagaagcc cataaagatc aaatcttagc
    21841 agaattttta aatcgtcatt ttagtttttc tcgtacgaat ggcttctcac ttaaagttaa
    21901 agtagaagat caaccacaat ggattaatgc tctaggagac tttatacaag cagttccagt
    21961 acgtgtagat gcattggtga tgagtaaatt acgaccattg ttgcactacg cgagagcgaa
    22021 agatatagac aactatagaa ctgtagaaga aagttatcgt caaggtcaat attaccgttt
    22081 tgatattgta gatggtaaat taaacattca attcaatgaa ggcgaaccat actttgaagg
    22141 cattgatatc gctaagccaa aagtgaaaat gacagcattc aaattcgaca accataaaat
    22201 tgttacagag ctaacgttaa atgaatttat gattggcgaa gggcaatatg atgtcagact
    22261 taaattacat tcacgaaaca aaaagcatac aatgtacgta cctttaagtg tcaatgcgaa
    22321 taaacaatat cgttttaaca ttatgttaga agatattaaa gcgtatttac ctaaagaaaa
    22381 aatttgggat gttttcttag aagtccaaat aggtacggaa gtatttgaag tgcgtgttgg
    22441 taatcaacgt aataaatatg cgtacactgc agaaactagt gcattaattc atttgaataa
    22501 tgatttttat agattaacac cgtatttcac aaaagacttt aataacattt cgttatactt
    22561 tacagctatt acattaacgg attcaatatc aatgaagtta aaaggtaaaa ataaaatcat
    22621 tttaactggt ctggatcgtg gttatgtatt tgaagaaggt atggctagtg tcgtgttaaa
    22681 agacgacatg gtgatgggaa tgttaagcca aacatcagaa aacgaagtgc aaatcttact
    22741 tagcaaagat attaaaaagc gagacttcaa aaatattgtt aagttaaata ctgcacatat
    22801 gacttactcg ctaaaataaa taataaatgc cctcaaatct atgtgaacta acatgatttg
    22861 agggctttat tatgctgttt atgacatgat tatgacattt cctcgtttat cattttcaaa
    22921 tacattaaat tgtatacact ggaaatgagg aggttatcta taatgataaa taaaaatgac
    22981 atagtagcag atgtagtaac tgattatccg aaagcagcgg atatttttag aagtgtggga
    23041 atagattttt gttgcggcgg acaagtaagt atagaagcag catccttaga aaagaaaaat
    23101 gtagatttga acgaattatt acagcgtctc aatgaagttg aacaaacgaa tacaccaggt
    23161 tcgcttaatc ctaaattttt aaatgtttcg tcacttattc aatatattca agcagcatat
    23221 catgaacctc ttagagaaga atttaaaaat ttaacacctt atgtgacgaa attatcgaaa
    23281 gtacatggac ctaatcatcc atatttagtc gagttaaaag aaacatatga tacatttaaa
    23341 aatggcatgt tagagcatat gcaaaaagaa gatgatgttg attttccaaa actaattaaa
    23401 tatgaacaag gtgaagttgt agacgatatt aatacagtga ttgatgattt agtatctgat
    23461 cacattgcaa ctggacaatt gttagtgaaa atgagcgatt taacatcaag ctatgaacca
    23521 ccgatagagg catgtggtac ttggcgactc gtttatcaga gattaaaagc acttgaagtg
    23581 ttaacacatg agcatgttca tttagagaat catgttttat ttaaaaaagt atcataaata
    23641 acgcgattag aaactgttgg caaaaataag tccagcagtt tttcgctatg tataaaagtc
    23701 ataatagtga catgaacatc attatttgaa aagaaaaatg atcaatatag cataagaaat
    23761 gatatgaaat tttaatgctt tagataatta aatcgtagcg tgatttttta ataatttatt
    23821 catgaatttt acatgcacta ttatgataaa ataaacataa ttataattca ctgaggtgct
    23881 atcgtgctat cgctaacaat gttattactt gagcgtgtag gtttaattat tattttggcc
    23941 tatgtgttga tgaatattcc ttattttaaa aatttaatga atcgtcgacg tacatggaaa
    24001 gcacgttggc aattatgtat tattttcagt ttgtttgcct taatgtctaa tttaactggt
    24061 atcgtcatcg atcatcaaca tagtttgtca ggaagtgtgt acttccgttt agatgatgat
    24121 gtatctttag ctaacacacg tgtattaacg ataggtgtcg caggattagt tggtggtcct
    24181 tttgtaggtc tatttgttgg cgttatttca ggtattttca gagtgtatat gggtggggcg
    24241 gatgcacaag tttatcttat ctcatctata tttatcggta taattgctgg ttattttggc
    24301 ttacaagctc aaagacgcaa gcgttacccg agtattgcga aaagtgccat gattggaatt
    24361 gttatggaaa tgattcaaat gttgagcatt ttaacatttt cccacgacaa agcatatgcg
    24421 gttgacctca tatcattaat tgcactacca atgattattg ttaatagcgt tggtacggcg
    24481 atttttatgt ctattatcat ttcaacatta aagcaagagg agcaaatgaa ggctgttcaa
    24541 acacatgatg tactgcaatt gatgaaccag acattgccgt attttaaaga aggattgaat
    24601 agagaatcgg cacagcaaat tgcgatgatt attaaaaatt taatgaaagt atctgcagta
    24661 gcaattacaa gcaaaaatga aatcttatcg catgtaggtg caggtagtga tcatcacata
    24721 ccaacaaatg aaatattaac aagtctgtct aaagatgtat tgaaatcagg aaagttgaaa
    24781 gaagttcata ctaaagaaga gattggttgt agtcatccga attgcccgct tagagcagct
    24841 atcgtgatac cacttgagat gcatggttct atcgtcggta cattgaagat gtattttaca
    24901 aaccctaatg atttaacttt tgtggaacgt caacttgcag aaggattggc aaatattttt
    24961 agtagccaaa ttgaacttgg tgaagccgaa acgcaaagta agttattgaa agatgctgag
    25021 attaagtcat tacaggcaca agtgagtcca cattttttct tcaattcaat taacacgatt
    25081 tcagctttag ttagaataaa tagcgaaaag gcacgagagt tactattaga attgagttat
    25141 tttttcagag cgaatttaca aggctcaaag caacatacga ttactttaga taaagagtta
    25201 agtcaagtgc gtgcatactt atcactcgaa caagcacgtt atccaggaag atttaatatc
    25261 aatattaatg ttgaagacaa atatcgagat gtgcttgtac caccattttt aattcaaatt
    25321 ttagttgaaa atgccatcaa acatgcgttt acgaatcgaa agcaaggtaa cgatattgac
    25381 gtgtcagtga ttaaagaaac tgcaacacat gtacgtatta ttgtacaaga taatggtcag
    25441 ggtatttcta aagataaaat gcatttgttg ggagaaacat ctgtagaatc agaatctgga
    25501 actggtagtg ctttagaaaa tttaaactta cgcctaaaag gattatttgg aaaatccgca
    25561 gcattacaat ttgaatcgac atcgagcggt accacttttt ggtgtgtact tccttatgaa
    25621 agacaagagg aggaataaat atgaaagcat taatcataga tgatgagcca ttagcacgta
    25681 atgaattaac atatttatta aatgaaattg gtggttttga agaaattaat gaggcagaaa
    25741 atgtaaaaga aacattggaa gcactactga taaatcaata tgacattata tttttagatg
    25801 tcaatttgat ggatgaaaat gggatcgaat taggagctaa aattcaaaag atgaaagagc
    25861 cacctgcgat tatttttgca actgcacatg accaatacgc agtacaggca tttgaattaa
    25921 atgcgacaga ctatattttg aaaccgtttg gtcaaaaacg tattgaacaa gcagtcaata
    25981 aagttcgtgc gactaaagcc aaagatgata atagcgcaag tacaattgcg aatgatatgt
    26041 cggcgaattt tgatcaaagt ttacctgttg aaattgacga taaaattcac atgttaaagc
    26101 aacaaaatat tattgggatt ggcacacata atggtattac aaccatacat acaacgaatc
    26161 ataaatacga aacaacagag ccattgaatc gttatgaaaa acgattgaat cccgcttatt
    26221 ttatacgtat tcatcgttca tatattatta atacgaaaca cattaaagaa gtgcaacaat
    26281 ggtttaacta cacttatatg gtaatattga caaatggtgt caagatgcaa gttggacgtt
    26341 catttatgaa agattttaaa gcgtcgatag gattacttta acagtaatcc ttttttttat
    26401 gcattttacc tatgatattt tgtatttcgg actaaaattc acgcaaatcg aagtgagcca
    26461 tctatacttt agttaaatca aacgtaggag gcaatggtcg tgaaacaaca aaaagacgca
    26521 tcaaaaccag cacacttttt tcaccaagtc attgtaattg ctttagtact ctttgtatcg
    26581 aaaataattg aatcatttat gccaattcct atgcctgcat cagtaatcgg tttagtatta
    26641 ttatttgtat tattatgtac tggtgctgtt aagttaggcg aagtcgaaaa agtaggaacg
    26701 acactaacaa ataacattgg cttactcttc gtaccagccg gtatctcagt tgttaactct
    26761 ttaggtgtca ttagccaagc accattttta atcattggac taataatcgt ctcaacaata
    26821 ctattactta tttgtactgg ctatgtcaca caaattatta tgaaagttac ttcgagatct
    26881 aaaggtgaca aagtcacaaa aaagatcaaa atagaggagg cacaagctca tgattaacca
    26941 cttagcacta aacacacctt acttcggaat actgttatcc gttataccat ttttcttagc
    27001 gaccatatta tttgaaaaaa caaatcgttt cttcttattc gcaccgctat ttgtcagtat
    27061 ggtatttggt gtggccttcc tctatttaac aggcattccg tataagactt acaaaatagg
    27121 tggagacatt atttacttct tcttagaacc agcaacaatc tgttttgcga ttccgttata
    27181 taaaaagcgt gaagtgcttg ttaaacattg gcatcgtatc atcggaggta ttggtatcgg
    27241 tacagttgta gcgttattaa ttattttaac ttttgcgaag ttagcacaat ttgccaatga
    27301 tgttatttta tcaatgttac ctcaagcagc aactacagcg attgcgttac cagtatcagc
    27361 tggtatcggt ggtataaaag aattaacatc attagcagtt attttaaatg gtgtcattat
    27421 ttatgcccta ggtaataaat tcttgaagct tttccgaatt actaacccta ttgcccgagg
    27481 attagcactt ggaacaagtg gtcacacatt aggtgtagca ccagccaaag aattaggacc
    27541 tgtagaagaa tcaatggcaa gtatagcttt agtgttagtt ggtgtagttg ttgtagcagt
    27601 tgtgcctgtc tttgtagcaa tattcttcta aaacgaaaaa cctaagcaag ataatagcag
    27661 tttgagccat tgttattatc gtgaaaaaac gtctatactc cgttttataa ctgggatata
    27721 gacgttttta tgtatgtact actttttact aggaattatg gtatttaggc gttcgtttta
    27781 tgtattgcca cttttgaaaa atggtagaac gaattaattt aactaggaat taaaatgtaa
    27841 gttatatcat ttgtgaaatt tcaattaaat atttttcgac aaattcttga aattacttaa
    27901 tgtcatcaac actttataaa aaaacggcgt atgcttatat gtaagaatgc aattaagagg
    27961 agattttata aatgagtatg caaagttcat ataaacgttt agttaaagtg ctactaacga
    28021 ttttagtgat tgtgttttca attttactag taggtggttg gtatattttt aaaaatgaag
    28081 cacctcgtcc aacaaagata gtagatcaac aaggtcatac gcttgttaca aaaggcgagc
    28141 tgatcagtgg gcaagcgata tatgagaaat atgggttaac agattatggc tcctacctag
    28201 gcaatggttc ttacttaggc ccagattata ctgcagaggc attacatcat tatctgattg
    28261 gtatgcgtaa atactatgca caagatattt atcataagca acttcataaa ttaaatcatg
    28321 tagagatagc cgtagttaaa gataaagtga tgaaagaaat acggattaat cgttattctg
    28381 aaaaagaaga tcaacttgta ttaacgccag gacaagtata cggtctaaaa tatttgcaag
    28441 attattataa aaaagagttt gttaataacc ctaaacaagt agggttaaat gaaaatatga
    28501 ttaaacagtt tcaaaatgat gattatatgg tcgcaggaaa taaaattgaa catttaagtc
    28561 aattcttttt ctggggtgct tggctatcat caacagatag acctggaaaa acattttcat
    28621 atacaaataa ttggccatac gatgtggatg caggaaatac gttgcctagt gcaggaattt
    28681 tatggactgc tatatctgta acattactga ttgcaggctt ggcattcatt atatatattc
    28741 aaaagcgtta tgaatttgac atgaagccga cgtatgagtc ggaacgggaa ctaccaaaga
    28801 ttgaagtaga tagtaaaatc actgatagtc aacgaaaagt tgggaaatat ttagtcgttg
    28861 tcatgttact atttttggtt caaatattat taggcgaatt actggcacat tattatgtag
    28921 agaataaatt ttttggtata gaaatacaaa ggctctttcc atttaatatt gtgaagacat
    28981 ggcatgttca attagttatt ttctgggttg ctacgacttg gttagcagca ggaatataca
    29041 ttgtacctaa agtattgggc aacgaaccaa agaaacaagg cgtattagta gatatcttgt
    29101 tttgggcctt aatcatagtt gtgatgggca gcatgattgg agaatggagt catattttag
    29161 gttggattga ttctcattgg tggctattcg ggcactttgg ttgggaatat atagaattag
    29221 gtaaattttg gcaaatatta tttatagtag gtatggtctt atgggttatc attctttgtc
    29281 gagggttttt accagctatt cgcaacaaag tacaagtgaa tcatgataaa cgtcacttat
    29341 tgacgctatt gtttgttgga gcaattgcaa ttccgttatt ttatcttgca tcattattta
    29401 taatgccgaa ttcacatgtg acgtttgcag attactggcg atggtggatt gttcacttat
    29461 gggttgaagg tatatttgaa gcatttgccg tagtgttaat tggtttctta atggttaata
    29521 tgaaattaac taccattgaa acgacgattc gtgcattata ttttcaatta atcctattac
    29581 taggtacagg tattgttggt atgggtcatc attactattg gcaaggtgat cattccatat
    29641 ggctagcact gggttcttgt ttttcagcat tagaagtcgt gccactttgt ttattaattt
    29701 gggaagcata cacacactat cgcttatata agaaaagtga catcgctttt ccttacaaag
    29761 gtacatttat ttttctagct tcaacaggat tgtggaatgc aattggtgct ggagcattag
    29821 gctttttaat taacacacca gcagttaact actttgaaca tggtacgcaa tggactgcag
    29881 cacatgccca tggttctatg gctggcgtgt atggtatgtt ctcaattgca attgcgttgt
    29941 atgtttttaa aaatattact aaaaaagaat tttggacacc gaaagttgaa aaatggatta
    30001 aaatatcatg ttgggcttta aatgttggat tggcaggtat ggtgttcgtt acattaatac
    30061 cagtaggtta tatccaactt aaagatggat tagaagatgg ttattggcat tcaagactaa
    30121 cgtcatttta tgaacaacca cttgtgaaag caatcatgtg gggtagaatg ccttgggaca
    30181 ttatattcac agtaggtgtc attatattag tagttatttt tataagggga tacagacact
    30241 taaaaccaaa atacagagta tagatcatca tattactgag tgatcttagt gtgttttaac
    30301 gtttgagacg aagagatatt atacaatagg attcaaacta taagttctta gattatgtaa
    30361 atataaagaa cagtgaaaag taattttctg attgaaaatg tacttctcac tgtttttaaa
    30421 gaaattatgt cctataaaac gtctatactc cggtttataa ctggaatata gacgttttta
    30481 tgtatttatt actttttact aggaatataa aactgtgcat gacgataatg aaatacgatg
    30541 tcagatgaat caaaaggttt gccagtcatt gtataaaacg tttggtgata acgtaaacat
    30601 ggttcacctg tagacaattg tagtaatgaa gcttcacttg aagtgagttg atctacatta
    30661 aagaaaatat ctgaaaaacc aatacgaagt ttcatgtttg attctaaata gtcgaagata
    30721 gagcccttag caatatcatc atttaaatat ttcacgattt ctttatgata ataagaatat
    30781 tcgatacata aaacatcatc gtccacgaat cttaatcgct ctaaatagta gacggtatca
    30841 tctgcattta attggagttc atcttgtaca gatttaggtg gcgttgcaat ctccttaaaa
    30901 acaagtacct tacttgtcat tcgatgttca cctaaacttt tagagaaacc attagtctta
    30961 aagacgttga tacgattggc atctgcaata tttctcacat aaataccact gccttgtgct
    31021 tgatagatca aaccatcttg ttccaataag cctaatgctt taatgatagt actcttactt
    31081 acttgataac gttcttttaa ttgcgtcacg cttggcaatt tatcaccggg tttgaaatta
    31141 gattgatgta taaacgcatt aagttgctta gcaatatgtt catactttaa caatattctg
    31201 tcctcgtttc actttgtctg taattattat agcacaaaat attataattg tatcggcgtt
    31261 tacaaattga accggtacaa ttataattag aggtaagtaa atacattttc attctactat
    31321 caagttgagg gggtaatatt tatgagtaat aaatataaag accaagccca agacattctt
    31381 acagctgtgg gtggtgtcga aaatattgtt gatgtttcgt atgatacggt acgcatgacc
    31441 attcatatgc atcatgcaat tccttctact gctaatgaag taacacaaat agatggcgtg
    31501 gcgtcagttg atgaaaatga aacacagtta gttatcgtat ttaatgaaga agtaaaatat
    31561 gtatatcaac aattacaatt gttaatggac gacgccaagc atcaagaaga tacgaatcat
    31621 gatgctgtgg atactcaaga aacagaacag aaagcgcaag caaaagtgac aacaccgatt
    31681 ttagtgaaag cacccattgc tggtcgtcgt atcttgctta aagaagttag agattcaatt
    31741 tttagagaga aaatggtagg cgaaggtttg gcgattaaag cgcataaaga atttaaagtg
    31801 attgcaccat ttagtggtaa agtctcaatg attgtaccta ctaaacatgc agttggtatt
    31861 caatcagaag acggtgtgga catagtcatt catattggtg tgaatacagt tgacttagaa
    31921 ggtaaaggat tcaagtgctt tgtaaagcaa aatgatcgtg ttgaagctgg gcaaacactg
    31981 ttacaattcg atcaacaata tattcaggaa caaggttaca actcagacgt tattgtcgtt
    32041 attagcaatt cttcggactt taacaaagta gaactgacaa tgaatgaaat cattacgact
    32101 gaagatgtta tttttaaaat atttaaaaac taggagtgtg ttgtaataat gacaaaatta
    32161 ccgcaaagtt ttatgtgggg tggcgcactt gccgcaaatc aatttgaagg tggatatgat
    32221 aaaggtggca aaggattaag tgtaattgat gttatgacga gtggtgcaca tggcaaagca
    32281 cgtcagatta cagaatctat agatccaaat cactattatc caaatcatga aggtattgat
    32341 ttttatcatc gttataagga agatattgcc ttgtttaaag aaatgggatt gaaatgttta
    32401 cgtacgtcga ttgcgtggac acgtatcttt ccgaatgggg atgaagatgt gccaaatgaa
    32461 gaaggacttg ccttttatga tcgtatcttt gatgaattaa ttgcacaagg tattgaacct
    32521 gttgtgacgt tatcacattt tgagatgcca cttcatttag cgaaacatta tggtggattt
    32581 agaaatagag aagttgtcga ttattttgtg cattttgcgc gtgttgtatt tgaaagatat
    32641 aaagataaag tgacatattg gatgacgttt aatgaaatta ataatcagat ggacacatca
    32701 aatcctatct ttttatggac gaattctggg gtagcattga cagaaaatga taatcctgaa
    32761 gaagtcttgt atcaagtagc acatcatgaa cttttagcca gtgctttagc agttcgtctt
    32821 ggtaaagaga ttaatccgaa gtttaagatt ggaacaatga tttcacatgt acccatttat
    32881 ccatattcgt gtcatccgaa agatatgatg gaagcacaaa ttgcgaatcg cttacgtttc
    32941 tttttcccag atgttcaagt gagaggttat tatccaagct atgctaaaaa aatgctggca
    33001 cgaaaaggat atgatgttgg atggcaagaa ggggacgaca gtattttaca gcagggcacg
    33061 gttgattata ttggctttag ttattacatg tctacggctg taaaacatga tgttaatact
    33121 acagttgaaa acaacatcgt caacggtggt ttgaatcatt ctgtggagaa tccgcatatc
    33181 gcaacgagtg attggggttg ggcgattgat ccagatggct taagatatac attgaatgtg
    33241 ttatatgatc gttatcagtt gccacttttt attgtggaaa atggttttgg tgcagttgat
    33301 gaagtggtag atggacatat tcatgatgat tatcgcattg aatatttaaa agcacatatt
    33361 acagcagcga tagaagcagt tgatcaagat ggtgtagatt taatcggtta tacaccatgg
    33421 ggaatcattg atattgtttc atttacaact ggtgaaatga aaaaacgcta tggtttaata
    33481 tatgttgatc gagataatga tggtcatggc acgatggaac gcttgaaaaa agattcgttc
    33541 tattggtatc aacaagtgat agcatcaaat ggagataaat tataaaggta tattataagt
    33601 attttagggt tagagtccga ggcgtaaatt aatatagtag gccctagagt gttatattgg
    33661 cggatcccca acacagaagc tggcgaaaat acagcttaca ataatgtgcg agttggcagg
    33721 cctccaacac agagaaattg gaacagcaat ttctacagac aatgcaagtt ggcggggccc
    33781 caacacagaa gctagcgaaa agtcagctta caataatgtg caagttggcg ggggccccaa
    33841 cacagaagct ggcgaaaagt cagcttacaa taatgtgcaa gttggggcag gacagaaata
    33901 aattttgcga aaatatcatt tctgttctac tccctttaac atttctgttc taataccttg
    33961 atgttaaaat tttaagaaaa aaataatgcc actaactaag tatgtatagt gttaatgttt
    34021 gagtgtttat aaaagtcttt aaccaaaatt gatgacttac taatcaacga taacgatatt
    34081 gttaagtaga tgattagtgt cacaaagaaa ttaaattgac aatcaaacat agatacaaag
    34141 tacagttagt ggcattatat ttagtgctct tttttagcga caaaagtaat ataattcata
    34201 tctttacgca gtctagtcat cgttttaaac attttacaaa acattggtcg attttctttt
    34261 tttaaagcat tgttgataat ctttatagtt ccaacaatac cttcgtcata aattaaacct
    34321 tttggtgtca ttaaactcat tggaccagta tgataatgca catgattaaa accagcttga
    34381 tgatataaat ctaaccagcc aagtttcgtc tgcggtgaga cattgacatt aattgctgca
    34441 gataatgatt taactacatg tgtggcatgt gattcattaa cgatgacaat atcatgtgtt
    34501 aacaagatac ctccaggctt taagactcgg tagtactcgc gtaatgcttt ttcctttatg
    34561 gcgatgggta acattgttaa cattgcttca tttaaaacga tatcgaattg attgtcatca
    34621 aagggcaatt taacagcatt cgcttgttga acttgaatat atgattcaag acctgctgct
    34681 gagatatttt cctgtgcttt ttctaaagct ttcttattta tatcaacgcc ttgaatgtga
    34741 cagccgtatg tatgagctag ataaatagat gtcgtacaca tattacatgc cacttctaac
    34801 acatgcttat cttgtgaaaa tgccccttgt tgtattaacc aatccgtggc tgctttacca
    34861 ccggggcgta gacgagtttt tcctaattta gctaaaaatg tatgaccagc ttctttagac
    34921 ataacgtaac ctccttatta attgataatc attatcatta atatagcata tttatatgcg
    34981 ataatttgaa agttaaacag atgacaaaaa attgtcactt catatataac taaacatgag
    35041 ttatcaagca taggtttagt ggaaaattat ttctaataga tgatgtttta taaaggtaaa
    35101 aaagcacagc catgatatac gaatgttgca tgttatacgc taaaccttca tatcatagct
    35161 gtgtttgatt catttaaact tgatttactt cttctagtag aggaatagat gcttgcgcgc
    35221 cgtgtttttg tacagtgagt gagctcgctt tattaccaaa atcaatagca tctgctaagt
    35281 tatcttgcga cttgtttaag cgactgacaa atgcaccaat aaatgtgtcg cccgcagcag
    35341 ttgtatcaat cgcatttact ttataagctt cgatgtgttg gctttgattt ttagtagcaa
    35401 aatatgtacc ttgcttacct aacgtaatca aaacagtctt aatgcctaaa gataaaaagt
    35461 aattagcatt gtctttcata gattgttcat tagttacttt aatcccagat aacaattcag
    35521 cttctgtttc gtttggcaca ataatatcga ttagtgacaa taattcatta ggtaatgctt
    35581 tcgctggtgc aggatttaat actgtcgtca caccatgtgc cttggcaatt tcaaatgcag
    35641 atataatagc cgggatgggt atttctaatt gtgcaacgac aaagtctgca ttgattatag
    35701 cgtcttttgc gttaataaca tcttcaggtg tcattgtcat attcgcacca ccataaacat
    35761 agatggtgtt ttgtccttct gcattcacag tgataaaggc ttgtcccgtt ttttcttcag
    35821 ttgttttgat aatatatgat gtatcaatat gagctgcttt aaaatcttct aagatgaaat
    35881 cagcaacgcc atcagtgcca attttagtaa taaatgttgt gtctgcttgc atgcgtgcag
    35941 tagcaatagc ctggttggca cctttacctc cgccgaatgc tttttgtgct tcttcaacat
    36001 gtaatgtttc gcctggttgt gcatatcttt caactgttaa aaattgatcg acattcgttg
    36061 aacctaaaat aacaactttg ttggtcatgt gtatgctcct ttcaagttat aatttttaaa
    36121 aagtaacatt cgattctaat gcaatattag agtagggcgt tgtttcacca gtacgaatat
    36181 tacctttatt taatgggtga gctaagttac ttttcatttc ttcgtgagga atgaaaatga
    36241 tttcgatttc cgatgaaatc aattgtttaa tttgttgcaa ttgtgtaggg ttatgttctt
    36301 ttatttcttc tgctaagtat attttttgga tttccatttc ttctaacact gtagctaaga
    36361 catcaataaa gcgtggtaag tttttagtta cagctaggtc gatacgacga tgatcatttg
    36421 gaattggcat gccagcgtca ttaatcgtta atagatcaaa atgaccaatt gtcgcgattg
    36481 cttttgaaat atgttcattt aaaacagctg tttttttcat gacatctaca ctccttattt
    36541 tataaatact gtaacagaag cggctactaa aatgagtact aagccgataa ttgtaataac
    36601 catttctttt gacgttttat gttgttttaa gaaataaata ccagttaatg tagcaagcac
    36661 aacggatgtt tgagaaagaa taaatccagt tgctaaacca ttcatattag gttgtgctga
    36721 aataagatat gttaaggcac caaatgcaaa gaagaaacct gaaataattt gtaaccacgt
    36781 aattttatta cggaatggat tctctgcttt catattcata aagccataaa tgactgcaac
    36841 aattaccata cccattgctt gaggtaaaaa ggcagttagg ccatcaatag aagttgcttg
    36901 cggtgcagct gaatataacc agtatccaaa ttcaccaatt aacagaagta ccactgcacg
    36961 acgtaaattt ttggcgttac ttgcttcttt gcgttcactc caaactgtca tacgcgcacc
    37021 aattagaata acgactaaag ctgtaaatcc aatgattcta tgaccaatgc ctggccaatt
    37081 tcctaatgca aagacacccc ataaagatgc gcctaataat tggaatgctg ttgtgactgg
    37141 catggcacga gatgaaccga ctaattcgaa cgctttaaat gtaatgattt gtccgaatcc
    37201 ccaaccagca cctgataata aggcgaatag caaattggtt ccagtaggga agccgcttga
    37261 tgtgactacg gctaatataa tagcgaagat taacgtacct acagtagcac cgataatttg
    37321 atgtacaggt ttaccaccaa actttgaagc gactgttggg aagaatcccc agccaattaa
    37381 ggggcctaac ccgataagta atgcaacaat gctcatgtta tacacctcac gatttgtttt
    37441 tagtaaaacg ttttaccagt gccatcgtat catttttatt tgtaaaatgc atgcgttttc
    37501 ataaaaactg aaaacgttta aatcatattt aaaaattcat acattattat tttaatttct
    37561 tagtgaaata attatgatta aaaaccctca gcattaaagc aacgctaagg gcataacaat
    37621 gatgagtata tttcggaaga tacgtagtta gtttgaaaga tgatagccag ttgttgcacg
    37681 gatttttaaa gtcgttggta attcaatcat atcaatggat ttatctaagt gctgtaatcg
    37741 ttgaagtaat aaggttaaag atgttttgcc aatatcagtt ataggttgtg ccacagtagt
    37801 taaaggtggc gagacgtacg ctgcataatc aatgtcgtca taacctatta atgagatgtc
    37861 tttcgggatg ctgatgccat gttcaattag tcctcgtaaa atgccgatag cgagttcatc
    37921 gttaatagcg aagattgcag tggcagattg aaccatgatg tcatcaacaa tggttagccc
    37981 accacgctta gataattcag tatggacgat ttgtggttct ggcaattgat tcgcgcgcaa
    38041 agtatcaaca aatccagtga cacgggtcga catattcgcc atcatgtcat atggtgcaac
    38101 aatcatcata ttgttgtgac cgagttctat taaatgttgt gctgcaagtt gtccgccttg
    38161 atattcattt gtccgaacaa aatctgtata gccttgatgg tcattttgat ccagtacgac
    38221 ataaggtaca tgatgtttct ttagatagtt atttagggcg tccggggatg aaatgtactg
    38281 tgcaataatt aatccgtcaa tacctcgatc aattaaatgt ttaatattgt catacaaatc
    38341 agttgcagta gatgttaaaa agcataaatc aacatcagat ggtttgtggt catgaatact
    38401 ttgcatcagt gctgagaaaa acggatttgt taagctaggc aaaatgacgc caatagtttg
    38461 aattttactg ccgcgcaatt gttttgcatg tttattaggg gcatagccta aacattctga
    38521 aacggcatgt acgtttttta tcgttgttgc ggaaaaacga ctatcattat gatttaaaat
    38581 atgtgacaca gttgtaactg atacaccagc ttctctagca acatctttaa ttgacacttt
    38641 tttcatatta tattccttct ctttattgtg taattaatca tacagttata tacccgatta
    38701 ttatattcat catatcatga taagcatttg atgacatatg aagaggtgca tgacttttat
    38761 tgtctacaaa cagacgtcta tagtatgcga ttaaggttga gacgagagaa tatattatta
    38821 aggttaaata acttttaatg attatgataa caagaatttt aaaatgttta ttgtgatgaa
    38881 aaatacaaaa gtcattattt taaagaataa aatatttgtt tttgaaaaaa gtattaaaaa
    38941 acataacaaa atgatgtata ttgaactatg tgattgaaaa aaattttaat taaggcttgt
    39001 taaaacttaa gagaggatgt ttttaaatgc aattcaaatt aaaagaagaa gagattatta
    39061 gttttttaga attgaaatat ccagaaaaag agttcgaata tggtcgtttg ttagttggac
    39121 aacataaacg tgatgattta gatgtttatt actttggtga tacgttttta atgtgcacga
    39181 ttatttcatt caagacattt gaaattaaag aaacagtaga attatcatat gatgctgtta
    39241 atcgtattgt gttaaaagat ggatggttat ttagaaaaat gagaatagaa acaatgcaaa
    39301 aagtgttaaa atacggtaca tctaaattaa tgttaactga ttttcaaaag gacaattata
    39361 ataaatatat tcaaggtcag aaacaacgcg tgatatttga aaatggccat tttgtctaat
    39421 tgatagtgaa taataattag agtaagcgaa gaacaataaa aaatgttctt cgcttatttt
    39481 tatagaaatt gatgcatatc attttaaagt gataaagata tttcgaaaca taaatcttct
    39541 attatattta agatttaact gtttttggaa tgatcatgta tgcagacaat gagccgagta
    39601 tcattaatac aatgctgacg ataaatgtta tagttgcagc tacacttggt gcatagttta
    39661 gttgtaacat actgaaaact gtagtactta gtgctatacc aaaggcgcca cctagtgtgc
    39721 cactcatttt atataatcct gtagctaaac caactttttc attaggcata ctgaaaattg
    39781 caattgtaag gccgggtgtt gcgactaaac catttccaat tgcgcaaatg acaaacccaa
    39841 taacaacagc aatgatatat tgtgatggtt gaagtgatgt catgctaata atagtgatgc
    39901 cgatgacagg gaacaacgga ccaatgatga gcatcaattt gccaccgaaa cgtaatgttg
    39961 ctttttcacc taaacgaatc atcgcaactg ccacaatggc atatggcaat gtaacaagtc
    40021 cagattgcgc agctgataaa ccaaggtgtg tttgagcata tatgaaaaag accactgtta
    40081 cgcctagacc actatttaaa acaaagttat ttaaaaatgc accaatgaac ggacggttgc
    40141 gtaatactga gaaatcaata aaaggtactt catgtcgacg ttcgatgatg atgaatatca
    40201 acgtagtgat gataataatg ctcagacaaa tgaatgaaaa tgtactaaac caaccttgtt
    40261 cgaatccttg tgttaacaat aatgtaaagc taccaatcat aacagcgaaa atagacatac
    40321 ctttgtaatc gaatggatga cgatggctat gttgacttac tttttcaggt gtgcctttta
    40381 gaagtaatat ggcaatgaaa gcaatgacta tactaatgat gaaattcgtt tgccatccaa
    40441 aatttgtggc gattaaaccg ccgataacac cagctaggcc gatgccacca acagtactaa
    40501 tcattagata actaatcgct cgtcttaaat gatctccttt aaattgatta tttaacacgc
    40561 caactgttga aggtaacaag atagctgctg atagaccttg taaaattcta ccgatgatga
    40621 gcagtgcagt gatgtccgat ataattaata gaagagatgc aaacatactg attatgagac
    40681 ccatgtatgt cattctcagt tgtcctattt tatcagcaat atcacctgca gccaccatga
    40741 agatacctgt ggcgaaggaa gttaaactaa tagataaatt taacacggca ggagaggttt
    40801 gatacgtttg accaacgaga gggcctatat taataaatga ttgtgcaaac aaccaatatg
    40861 ttaatgcaga caacataatc gcaataataa tattactgcg cggtgaagat tgtgtgttat
    40921 tcacagatgt cactccttgt aaaaattaaa tatagaaagt catgagatga agtgaacgaa
    40981 tgtatagatg atcgtacact cacacataag tcatgatttc cttacatatc ataccacagt
    41041 tattgagaat gaatatcaat taattgcttg tatgattgat tttttatagt atgtttaagt
    41101 gaaacagctt tatttgaaag tgattaaagt aaatgaaggt acagaggagt gaggacaatg
    41161 tgcacaggat tcacaataca aactttaaat aatcaagtac ttcttgggcg cacaatggat
    41221 tatgattatc cattagatgg ttcgccagca gtgacgccta gaaattatcg ttggacatct
    41281 cgcactggca cgacaggcca aacacaatat ggctttattg gtacaggaac agatatggaa
    41341 ggttttattt atggtgatgg tgttaatgaa catggcgttg ccatttcaac acaatatttc
    41401 cgaggttata gttcatatgg atcaatacac aaagcggacg cgatgaatat tacgcaaaat
    41461 gaaattgtga catggatttt gggatataca acaagcattg aagatatgaa acaacaagca
    41521 tcccaaatac atgttgtagc tgtatattta aatgatatcg gtgaagttcc gccattgcat
    41581 tatcatgttt ccgatgcaac tggacattca gtcgaagttt catttaaaga gggggaagtg
    41641 gttataaaag ataaccctat tggtgtctta acaaatcatc cagacttaga ttggcattat
    41701 agtaatttaa gacaatatat caatatttct ccttatccag caccagcaaa tttattggaa
    41761 ggtgtaacga ttgaaccttt aggcaatgaa gcaggtacat ttggattgcc aggtggattt
    41821 acttcaactg agcgctttgt gagaatggca tttatgaaag caaacattgc tcaaaacaat
    41881 gataaagaaa tggatttaat gaatgcattt tatttattag atgcggtaaa tataccgatt
    41941 ggaattgtac gtccgcatga tgctgacaat cactatacga tgtatcagac cgtaataaat
    42001 ttaactacaa gaacgttata tattaagtat tatggcagca atgaaatagt agcattaaag
    42061 ctcacagatg atttaattaa tagaaaagat atgacgattt ttaagcctga gaagcatatc
    42121 actattagaa agttgaatga caatcaatag cgattgataa tggaatttgg ttgatatgat
    42181 ttgatggatg attactatca tacattagcg ttgaatcgtg aacatggacg aatgattagt
    42241 tgttaatttt gaaaatttat aaaaggttaa gcgaacgcag tgagagatgt cattttaata
    42301 tcaatgtatg gctattttgt aatgacaatg taatgagttt agtaaaacat ttcgggaata
    42361 ttaaatagtt ggaaaatgag aattaaatcc tttactcagt tgtctaattc ttttagtatg
    42421 tgcagtacag tttaattgaa aactaacttt aactttaatg gaggatgttt tatacatgaa
    42481 aaaattaaca gcagcagcga ttgcaacgat gggcttcgct acatttacaa tggcgcatca
    42541 agcagatgca gcagaaacga caaacaccca acaagcacat acacaaatgt caacacaatc
    42601 acaagacgta tcttatggta cttattatac aattgattct aatggggatt accatcacac
    42661 acctgatggt aactggaatc aagcaatgtt tgataataaa gaatatagct atacattcgt
    42721 agatgcacaa ggacatacgc attattttta taactgttat ccaaaaaatg caaatgccaa
    42781 tggaagcggc caaacatatg tgaatccagc aacagcagga gataacaatg actacacagc
    42841 gagtcaaagc caacagcata ttaatcaata tggttatcaa tcaaatgtag gtccagacgc
    42901 gagctattat tcacatagta acaacaacca agcgtataac agccatgatg gtaatggaaa
    42961 ggtcaattac cctaatggca catctaatca aaatggcgga tcagcaagta aagcgacagc
    43021 tagtggtcat gcgaaagacg caagctggtt aacaagtcgt aaacaactac aaccatatgg
    43081 acaatatcac ggtggtggtg cgcattacgg tgtcgactat gcaatgcctg aaaattcacc
    43141 agtttactca ttaactgatg gtacagtagt acaagcaggt tggagtaact atggtggcgg
    43201 taatcaagta acgattaaag aagcgaacag taataactac caatggtata tgcataataa
    43261 tcgtttaact gtttcagctg gtgataaagt caaagctggt gaccaaattg catattcagg
    43321 tagtacgggt aattcaacag cacctcacgt acacttccaa cgtatgtctg gtggcatcgg
    43381 taatcaatat gcagtagacc caacgtcata cttacaaagt agataataca gtaaatccca
    43441 agttacgata tcatacgcaa cttgggattt tttcgtttta ataataggta taagcccatt
    43501 gcttgtcctc taaaacatgt tgataaaaag gatcttggcc aattaacttg atatcatctg
    43561 caagtgcttc aacttcatct aaatgatggg tagttaatat aattaaacat ttagatttca
    43621 taatgttaag tagttggtgg atgtcatgtc tagatttcaa atcaatacca actgtcggtt
    43681 catctaaaat gagaattcga ggttgaccta gtaaacctac taatatatta attttacgtt
    43741 tatttccacc ggacaatgta gatactttgg cagacgtatc attaaagttt aattgctgta
    43801 aatattcgtt gatagttgta tcgttaattg aatttttaca aagtgattta aaaaatttaa
    43861 tgttttcagc cactgtcatg tgttcaaata acgcaatgtc ttgtggcaca taaccgatgt
    43921 gattttgtat ttgtctttga ttccattttt cgccgaaata gttgatagtt ccatcattag
    43981 ctttttcaat accagcaatc atacgaagta atgttgattt tccagcgcca ttatcaccaa
    44041 gtaatacggt taaacgatta ctatcaaagg acatggttaa atgattgaaa atctgtttgt
    44101 tacggtaacg ctttgaaagg ttattaattt ctatcatcag ttacactcct ttacatgaaa
    44161 atggtcaagt atacgatacc catagcaagt gcatatataa atgtcatgaa taatcgatga
    44221 cttattgttt gaatatggaa taagataaag acgataccta tctcataaat caatataagt
    44281 aacagtgatt ttaagtaaaa tatcaagctg agtggttgag acaaatatag actaactgcc
    44341 aatagtacca acaataataa gatcgtatgt gtcattacat aagtactata tagtttgaaa
    44401 cggcttaaat gatattgtga taatcgttgc aatgctgctt gttggtttaa acgataatga
    44461 agtactactt gaacagcgct aacaaataaa atcaccgcaa agattaagct aattgaaata
    44521 gagtgttgtg cttgtttagt aagcgacaca aatttgattt tagattcagg tgtatgttta
    44581 tgataggact tgttgatagc atcgatggat tgatgctgtt tcatatcctc aaggtgttca
    44641 taaataatgt taggaatttg ctgttcatat aatgagctac tgacaatttc tacagcaata
    44701 ccacctataa agtcatctct accatataac tgtatcgttt cttttaaacg gttctctttt
    44761 aatttttgag agaaaccttt aggaatttgc atacttaaaa tagcttcctt tttagtaaca
    44821 tcatcttcaa tatagctttc atcttcatcg acttttttaa tagttacata gtcagattgt
    44881 ttaattttat tgacgaatga ttttgatgca gtggtttggt ctaaatcttg aatggtaatc
    44941 ggtattttga agttgtcatg tgctacacgg taacccatac caataagtac gagtgcggtg
    45001 acaatggttg ttacgagcaa gatgtattgt aaccattgct tgaacacaac aagttgtata
    45061 taaggcttca ttgacgatac ctccaaacca atacagctaa attaattaac aaaagtgcga
    45121 tgaagctaag atagaagcta gggtgcagtt ctaaaatgta gttgtttaaa ataatttcta
    45181 acaattgatt tgttacaact gcgaacggtt gaatattgaa aacgccattt gctatatgtt
    45241 gtaaaaaaat cgtaggtatt gttaaaccag ataacaccag gatgacaata gctaatatga
    45301 ctttactaat actattcaac aagcctgttg ttaaaagttc gatgattaat aaccacagta
    45361 ttaaaaaggt aacataataa cttaaatgaa tggctaacgt tggccaatta tataattcaa
    45421 aggtattcgg aatactgaat ataatccaaa ctacaccaac ggtactccat aacatagtat
    45481 aaaaccatgt aatcaacgca cgaatgatta ataaacgctc tttagaaaaa tgaaacattt
    45541 tcaatcgcgc tttcaataca gtatcttgat tcattttcaa aactgtaaat aaagatagtg
    45601 caaagatgaa taccgttgct aaaaatcctg taattgcata ataactgccc gtatcgtata
    45661 aatgaatcgg ttctaagtta aatgcacctg aacggtttaa tcctgtaatc agcaaatcag
    45721 tcataacatt gatactgtca gaatgtgatg ctttcggtgc taagtcttga aaagctaaga
    45781 tgccacccat tgatcgcata agacgttggt aaacagaatc tgttagctga gatagcacga
    45841 cacttttcat ggattgttga tcatatgtat atactgaaat tggtagttcg ccttgtttat
    45901 aaaatgcctt ggtcatacct ttatcaaaaa caaaatagcc ttgaagttta tgttttttta
    45961 acaaagtatg tgcttgctta tcatcatatg ctttaatgct tacatttttt cctaggttac
    46021 tccctttacc aatagagttt aagattaatt tcgtttcact tgattgatct ttatctacga
    46081 cacctatatt aaaatgattg tcatcttctg ttacatgttg gatcgtcgtt aatgtaataa
    46141 ggagtgccgc taatataaat agtaaataga taatcaaata ccactttttc aataaaaaag
    46201 agtggtagat gcgaaacaaa tgtattgttt tcatttaacc actcctccaa tgataagatt
    46261 gaaaggcaag atgaccttcc aatcttattt ttatatttta gttatttaga tgcctttttt
    46321 aaaattgatt caaacatttt gccgccattt ttttcgattt ctttttcaag tttttcacgg
    46381 tcttttgaag atagactatt gaaatctttc gcaccactat catcaaaatc aatatctgct
    46441 ttcaattttg tgctagattt taaaatgaaa ttaattggtt cttcagcata tttgatgccg
    46501 atatttaacg tagatttctg agtgttattt tttacgtcag aatctatatt attttcaaaa
    46561 gtgaattcat tttcgtcgct atatttatct aatgcgacag tgattttacc tttatcttga
    46621 cgttttgtgc catctacttt ttcttggtta tctaatttga tttttgattc atcatattct
    46681 gtctttttac caaattcgta tttatcatta tatttattat ctttttcttt agaagatacg
    46741 cctttaattg tatatttcgc ttcagcatac gtgtatttat cttgatcgaa atcaagtgcg
    46801 taatctagtt ttaacttatc gtcttctaaa gtattagtac ctttgatttt agttttgtta
    46861 ttttctttgt ctgtaatagt aatttctcgt tttacaatcg tatgtttttc ggtataaatt
    46921 ttagattgaa ttttagcaaa ttcatccttt ttagtttctt tgacatcgtc aattgctttt
    46981 ttgatgtctt tttcaaagtc ttttgtagca ccttgttctt ccattaattt tttaaggtct
    47041 ttatcctttt tagcttcttc taatacagct aatgtaattt ttttagtgtc agctctgcta
    47101 agtgttaacg tgacaggtct aactttgtac ttttcaccat taaccttaat ttcttctttt
    47161 ttacctttat caaaattatc gtcatctaat ttgtcgacaa taagttcgga atatttttcg
    47221 gcaattttgc tgtagtcact ttgttgtgct tgagcattac taaaaagagt atttaaattt
    47281 agttgttggt ttgtaatacc attttctttt gctgtttctt catcttcacc tgtaagtttt
    47341 gaataagttg ataataaatc agaattatta acactatatt tccctttaaa taatggtgat
    47401 tcgaaataat gcttatcttt atctgcagct aactggaatt tccctaatgc agagtctgct
    47461 attgttggtt caagattaat cattgatttc tcttttttag gatcatgtcc atatgacatt
    47521 ttaattttcg aagcattaac aacagattta ggaataccaa gccccttaac aatttcatct
    47581 gatgcatctg cgcttaattc taatgaagat aaaaatgaat tatctttcat cttttcttgg
    47641 aacttcactt cattttcaaa acggtcatta aaataatctt tatacatttt tgctgtttgt
    47701 tgttcacttt ttaggtatgt atttttcggt gtattagcaa aaaatgcata aactccccat
    47761 gcgattccac ctattaataa taagacaaca ataataggaa ttataatttt tagtttttta
    47821 gacattttgt ttcctcccaa ttatttatga gttaatcata tcagaacaat acattttatt
    47881 aaatggttta ttttaaattt tgtttaaatt aataggattt tagttataat aatgtttaat
    47941 aagttattgg aaatctaata aactacaaaa aatattttga ttacataatg attatttaaa
    48001 aatgtcggtt aactttaagg tgacaagtta ttgcaatttt gaaggtagac atcatatttt
    48061 ataatcgaat aacatacaaa ataaagcgac caattttgca gtgttacgaa ttgtaaactg
    48121 cgaactggtc gctttttatt gatgtgatga ttaatttaat ggatgtaatt atatgatgaa
    48181 acttctgaag cagagatggt tcttgatgaa acgatatatt caccaatcca attcatttct
    48241 gaaattagaa tacttccatc aatattaact ttttcaacgt aggctacatg accaaatgga
    48301 ccatttactg tttgtaaaat tgatcctcgt gttgggtgtc tatctacttt gaagccattg
    48361 cttgaagctt ggcctgccca gtttttagca tctccccaaa atgtactaat cgtgtgtcca
    48421 tctttggcac gtttatcaaa gacataccat gtacattgtc cagcagtata taagttgttc
    48481 ttacttgtga taagaggctg atcaatgatt ttaccgttac ctaatgctaa aggtttaccg
    48541 tcagcagtct ttgctttgtc attaaattcg gcgatttgta attcgtcata caattcgtct
    48601 aaatcgatgc ctgtaataag ccctttgtta tcttttgaaa aagcgttatt taattcatcg
    48661 tcattgtctt cgacattcgg tattgctggt gtcaaaggat tgcttggtga cgtttgaggc
    48721 ggtgtgtgtg aatcaattgc gtcattaatg tgcgtatact gaccacttaa tgaagaatgg
    48781 tactgattgt tgttaagatc acgttgattt gcgtgttgat gattgtcggt tgtacgtgac
    48841 tgattttgat gattgttgtt tggcgcgttg tttttgtcat aagtataagt atacgcgccg
    48901 gtgtctttat tcactttgaa ctgtgcgttt gggtgtgctt tctttgcttc ttctaatgtt
    48961 ttgctatcat tcgtgtatgc ttgagccgag ttaggcgaca tactaaataa agtaagagtt
    49021 gtcatcgtca gtaaaattgt tttcttcata ataaccattt aatcctttat gtatttaatt
    49081 taattttagt atacacattt atattacaaa aatgaatggt taattaaaaa tatatgggta
    49141 tattcaatat atttatttaa aaaaagctaa aaatacttaa aaaactatat acatatacta
    49201 ataatttata tattatttga gtaaggagca ctttttcaaa aaatagtgtc cctaaaaagt
    49261 tttgataaac ttaaaatatt caggaggttt ctagttatgg caatgattaa gatgagtcca
    49321 gaggaaatca gagcaaaatc gcaatcttac gggcaaggtt cagaccaaat ccgtcaaatt
    49381 ttatctgatt taacacgtgc acaaggtgaa attgcagcga actgggaagg tcaagctttc
    49441 agccgtttcg aagagcaatt ccaacaactt agtcctaaag tagaaaaatt tgcacaatta
    49501 ttagaagaaa ttaaacaaca attgaatagc actgctgatg ccgttcaaga gcaagaccaa
    49561 caactttcta ataatttcgg tttgcaataa gcattctgaa attggcaaag tcacattttc
    49621 taatgtggct ttgcttatca ttttttaaga aaacaactga aaggaaataa gcatgaaaaa
    49681 gaaaaattgg atttatgcat taattgtcac tttaattatt ataattgcca tagttagtat
    49741 gatatttttt gttcaaacaa aatatggaga tcaatcagaa aaaggatccc aaagtgtaag
    49801 taataaaaat aataaaatac atatcgcaat tgttaacgag gatcaaccaa cgacatataa
    49861 cggtaaaaag gttgagctgg gtcaagcttt tattaaaagg ttagcaaatg agaaaaacta
    49921 taaatttgaa acagtaacaa gaaacgttgc tgagtctggt ttgaaaaatg gcggatacca
    49981 agtcatgatt gttatcccag aaaacttttc aaaattggca atgcaattag acgctaaaac
    50041 accatcgaaa atatcactac agtataaaac agctgtagga caaaaagaag aagtagctaa
    50101 aaacacagaa aaagttgtaa gtaatgtact taacgacttt aacaaaaact tagtcgaaat
    50161 ttatttaaca agcatcattg ataatttaca taatgcacaa aaaaatgttg gcgctattat
    50221 gacgcgtgaa catggtgtga atagtaaatt ctcgaattac ttattaaatc caattaacga
    50281 cttcccggaa ttatttacag atacgcttgt aaattcgatt tctgcaaaca aatatattac
    50341 aaaatggttc caaacataca ataaatcatt attgagtgcg aattcagata cgttcagagt
    50401 gaacacagat tataatgttt cgactttaat tgaaaaacaa aattcattat ttgacgagca
    50461 caatacagcg atggataaaa tgttacaaga ttataaatcg caaaaagata gcgtggaact
    50521 tgataactat atcaatgcat taaaacagat ggacagccaa attgatcaac aatcaagtat
    50581 gcaagataca ggtaaagaag aatataaaca aactgttaaa gaaaacttag ataaattaag
    50641 agaaattatt caatcacaag agtcaccatt ttcaaaaggt atgattgaag actatcgtaa
    50701 gcaattaaca gaatcactgc aagatgagct tgcaaataac aaagacttac aagatgcgct
    50761 aaatagcatt aaaatgaaca atgctcaatt cgctgaaaac ttagagaaac aacttcatga
    50821 tgatattgtc aaagaacctg atacagatac aacatttatc tataacatgt ctaaacaaga
    50881 ctttatagct gcaggtttaa atgaggatga agctaataaa tacgaagcaa ttgtcaaaga
    50941 agcaaaacgt tataaaaacg aatataattt gaaaaaaccg ttagcagaac acattaattt
    51001 aacagattac gataaccaag ttgcgcaaga cacaagtagt ttgattaatg atggtgtgaa
    51061 agtgcaacgt actgaaacga ttaaaagtaa tgatattaat caattaactg ttgcaacaga
    51121 tcctcatttt aattttgaag gcgacattaa aattaatggt aaaaaatatg acattaagga
    51181 tcaaagtgtt caactcgata catctaacaa ggaatataaa gttgaagtca atggcgttgc
    51241 taaattgaaa aaggatgctg agaaagattt cttaaaagat aaaacaatgc atttacaatt
    51301 gttatttgga caagcaaatc gtcaagatga accaaatgat aagaaaacaa cgagtgttgt
    51361 ggatgtaaca ttgaatcata accttgatgg tcgcttatcg aaagatgcat taagccagca
    51421 attgagtgca ttatctaggt ttgatgcaca ttataaaatg tacacagata caaaaggcag
    51481 agaagataaa ccattcgaca acaaacgttt aattgatatg atggttgacc aagttatcaa
    51541 tgacatggaa agtttcaaag acgataaagt agctgtgtta catcaaattg attcaatgga
    51601 agaaaactca gacaaactga ttgatgacat tttaaataac aaaaagaata caacaaaaaa
    51661 taaagaagat atttctaagt tgattgatca gttagaaaac gttaaaaaga catttgctga
    51721 agagccacaa gaaccaaaaa ttgataaagg caaaaatgat gaatttaata cgatgtcttc
    51781 aaatttagat aaagaaatta gtagaatttc tgaaaagagt acacaattgc tatcagatac
    51841 acaagaatca aaaacaattg cagattcagt tagtggacaa ttaaatcaat tagataataa
    51901 tgtgaataaa ctacatgcga caggtcgagc attaggcgta agagcgaatg atttgaaccg
    51961 tcaaatggct aaaaacgata aagataatga gttgttcgct aaagagttta aaaaagtatt
    52021 acaaaattct aaagatggcg acagacaaaa ccaagcatta aaagcattta tgagtaatcc
    52081 ggttcaaaag aaaaacttag aaaatgtttt agctaataat ggtaatacag acgtgatttc
    52141 accgacatta ttcgtattat tgatgtattt actatcaatg attacagcat atattttcta
    52201 tagttatgaa cgtgccaaag gacaaatgaa tttcattaaa gatgattata gtagtaaaaa
    52261 ccatctttgg aataatgtca ttacgtcagg tgttattgtt acaactggtt tggtagaagg
    52321 attaattgtc ggtttaattg caatgaataa gttccatgta ttagctggct atagagcgaa
    52381 attcatctta atggtgattt taactatgat ggtcttcgta cttattaata cgtatttact
    52441 aagacaggta aaatctatcg gtatgttctt aatgattgct gcattgggtc tatactttgt
    52501 agctatgaat aatttgaaag cggctggaca aggtgtgact aataaaattt caccattgtc
    52561 ttatatcgat aacatgttct tcaattattt aaatgcagag catcctatag gcttggcact
    52621 agtaatatta acagtacttg tgattattgg ctttgtactg aacatgttta taaaacactt
    52681 taagaaagag agattaatct aatgttgatg aatagcgtga ttgctttaac ttttttaaca
    52741 gcatctagca ataatggcgg acttaatatt gatgtgcaac atgaagagga aaagcgaatc
    52801 aataatgatt taaatcaata tgatacaacg ctatttaata aagatagtaa agaagtcaac
    52861 gacgcgattg ctaagcagaa aaaagaacga caacaacaaa taaaaaatga tatgtttcaa
    52921 aatcaagcga gtcactcgac tcgcttgaat gaaactaaaa aagtgttatt ttccaaatct
    52981 aacttagaaa agacttcgga gagtgataaa agcccctata ttcaaaacaa gcaggagaaa
    53041 aaaatattcc cgtacatttt gatgtctgta ggggcttttt tgactttagg atttgtcatt
    53101 ttttcaattc ataaagggag acgaacgaaa aatgaatcag cacgtaaaag taacatttga
    53161 ttttactaat tataattacg gcacatatga cttagcagta ccagcatatt taccgataaa
    53221 aaatttaata gctttagtat tggatagttt ggacatttca atatttgatg tcaatacaca
    53281 aattaaagtg atgacgaaag gtcaattact tgttgaaaat gatcgactca ttgattatca
    53341 aatcgctgat ggagatattt tgaagttact ataggaggaa aaatagatgg ttaaaaatca
    53401 taaccctaaa aatgaaatgc aagatatgtt aacaccttta gatgccgaag aagcagctaa
    53461 aacaaaatta cgtttagata tgagagagat tcctaagtct tcaattaaac cagaacattt
    53521 tcatttaatg tacttattag aacaacattc tccatatttt atagatgctg aattaactga
    53581 actacgtgac agtttccaaa tacattatga cattaatgac aatcatacac cttttgataa
    53641 tattaaatca tttactaaaa atgaaaaatt acgttactta ctcaatatca aaaatttaga
    53701 agaagtaaat cgtacacgct acacatttgt gttggcacca gatgaattat ttttcacaag
    53761 agatggatta ccaattgcta aaacaagagg tttacaaaat gttgttgatc cattaccggt
    53821 gtcagaagct gaatttttaa caagatataa agcactggtt atctgtgcat tcaatgagaa
    53881 acaatcattt gatgctttag ttgaaggaaa cttagaacta cataaaggaa cgccatttga
    53941 aactaaagtt attgaagctg caacgttaga tttactaacg gcatttttag atgaacagta
    54001 tcagaaacaa gaacaagatt atagtcaaaa ttatgcatat gtacgcaaag taggacatac
    54061 cgttttcaaa tgggttgcta tcggtatgac aacgttaagt gtgttattaa ttgcattctt
    54121 agccttttta tatttttcag taatgaagca taatgagcgc attgaaaaag gataccaagc
    54181 atttgtgaag gaagattata cgcaagtact aaatacgtat gatgatttag atggaaaaaa
    54241 attagataaa gaagcgcttt acatttatgc caaaagctat attcaaacaa ataaacaagg
    54301 tttagaaaaa gataagaaag aaaacttgct taataacgtg acaccaaatt caaacaaaga
    54361 ctacttatta tattggatgg aattaggaca aggacatctt gatgaagcga ttaatatagc
    54421 cacttattta gatgataacg atattacaaa gttagcgttg attaataaat taaatgagat
    54481 taaaaataac ggagatttat cgaatgataa acgttctgaa gaaacgaaaa agtataacga
    54541 taaattgcaa gatattttag acaaagaaaa acaagttaaa gatgaaaaag cgaaatctga
    54601 agaagagaaa gcaaaagcaa aagatgagaa attaaagcaa caagaagaga acgaaaagaa
    54661 acaaaaagaa caagcacaaa aagataaaga aaaacgtcaa gaagctgaaa gaaaaaaata
    54721 gtataggact gaggcaaaga caatgcataa attgattata aaatataaca aacaattgaa
    54781 gatgctcaat ttgcgagatg gtaagacata tactattagc gaagacgagc gtgcagatat
    54841 tacgttgaaa tcgttaggcg aagtcattca tttagaacaa aataatcaag gtacttggca
    54901 agcgaatcat acttctatta ataaggtgct tgttagaaaa ggtgaccttg atgacattac
    54961 attacaactt tatacagaag ctgattatgc atcatttgct tatccttcaa ttcaagatac
    55021 gatgacaatt gggccaaatg cgtatgatga tatggttatt caaagcttga tgaatgccat
    55081 cattattaaa gattttcaat caatacaaga atcacaatac gtacgcattg tgcacgataa
    55141 aaatacagat gtgtatatta actatgaact acaagagcaa ttaacgaaca aagcttacat
    55201 tggtgatcat atttatgttg aagggatatg gctcgaagta caagctgatg gtttaaatgt
    55261 attgagtcag aatacagtgg catcgtcatt aattcgctta acacaagaga tgccacatgc
    55321 acaggcagat gattacaata cgtaccatcg ttcgccaagg attattcacc gtgaaccgac
    55381 cgatgatatt aagattgaaa gaccgccaca gccaatacag aagaacaata cggtgatatg
    55441 gcgttccatt ataccgccat tagtgatgat tgctttaact gttgtcatct ttttagtgag
    55501 accaattggt atttatattt taatgatgat tggtatgagt acagtaacga tagtatttgg
    55561 tattacaacg tatttctctg aaaagaaaaa gtataacaaa gatgttgaaa aacgagagaa
    55621 agattacaaa gcttatttgg ataataaatc taaagaaatt aataaagcga ttaaagcaca
    55681 acgttttagt ttgaattacc attatccaac ggttgctgaa attaaagata tcgttgaaac
    55741 gaaagcacca agaatatatg aaaaaacatc gcatcatcac gatttcttac attataagtt
    55801 aggtattgcg aatgtagaaa agtcattcaa attagattac caagaagaag aatttaacca
    55861 acgtcgtgat gaactattcg acgatgctaa agaattgtat gaattttaca cagatgtaga
    55921 acaagcacca ttaatcaatg atttaaatca tggaccaatt gcatatattg gtgcacgaca
    55981 tctcatttta gaagaattgg agaaaatgct aatccaattg tcaacgttcc atagttatca
    56041 tgatttagag tttctatttg tgacacgtga agatgaagtt gaaacattga aatgggcacg
    56101 ttggttgcca catatgacat tgagagggca aaacattaga ggatttgttt acaatcaacg
    56161 aacgcgtgac caaattttaa cgtcaattta tagcatgatt aaagaacgta tccaagctgt
    56221 gcgtgaacgc agcagaagta atgagcaaat tattttcaca ccgcaattag tgtttgtcat
    56281 tacagatatg tcattaatta ttgatcatgt cattttagaa tatgtaaacc aagatttatc
    56341 agaatatggt atttcattaa tctttgttga agatgtgatt gaaagtttgc cagagcatgt
    56401 agataccatt attgatatca agtctcgtac tgaaggcgaa ctgattacga aagaaaaaga
    56461 attagttcaa ttgaaattta cacctgaaaa tattgataac gtcgataaag aatatatcgc
    56521 gcgacgtttg gcgaatttga tacacgtcga acatttgaaa aatgcaattc ctgatagtat
    56581 tacattttta gagatgtata acgtgaaaga agtagatcag cttgatgtag ttaatcgatg
    56641 gagacaaaac gaaacataca aaacgatggc agtaccttta ggtgtaagag gtaaagatga
    56701 tattttatca ttgaacttac atgaaaaagc acacgggcca catggtttag ttgctggtac
    56761 cactggttca gggaaatctg agattatcca atcatacatt ttatctttag ctattaattt
    56821 tcaccctcat gaagttgcat tcctattgat tgactataaa ggtgggggta tggcgaactt
    56881 atttaaagat ttagtccatt tagttggtac gattacaaac ttagatggtg atgaagcgat
    56941 gcgtgcctta acatcaatca aagccgaatt gagaaaacgt caacgtttat tcggagagca
    57001 tgacgttaac catattaatc aataccataa gttatttaaa gaaggtgttg cgacagaacc
    57061 aatgccacat ttattcatta tttccgatga gtttgccgaa ttaaaatcag aacaacctga
    57121 ttttatgaaa gaacttgtat caacggcacg tattggacgt tcgttaggta ttcatttaat
    57181 acttgcgaca caaaaaccat cgggtgttgt tgatgaccaa atttggtcta actctaaatt
    57241 taaattggca ttaaaagtac aagatagaca agacagtaat gaaattttaa aaacaccaga
    57301 tgcagcagac attacattac caggtcgtgc gtatttacaa gttggtaata atgaaattta
    57361 tgaattgttc caatctgcat ggagtggtgc aacatatgac atcgaaggcg ataaattaga
    57421 agttgaagat aagacgattt acatgattaa tgactatggt caacttcaag caatcaacaa
    57481 agacttgagt ggacttgaag atgaagaaac aaaagaaaat caaactgagt tagaagcggt
    57541 tatcgatcat atcgaatcta ttacaacacg attagaaatt gaagaagtta agcgaccatg
    57601 gctaccacca ttgccagaaa atgtatatca agaagatttg gtagaaacag atttcagaaa
    57661 attatggtca gatgatgcaa aagaagtgga attaacatta ggacttaaag acgtaccaga
    57721 agaacaatat caaggaccga tggtattgca attgaaaaaa gctgggcaca tcgcgttaat
    57781 cggaagtcca ggatatggta gaacaacgtt cttacacaac attattttcg atgttgcaag
    57841 acaccatcgt cctgatcaag cacatatgta cttgttcgat ttcggtacca atggtttgat
    57901 gccagtcaca gacataccac atgtcgctga ttactttaca gtagatcaag aagacaagat
    57961 tgctaaggcg atacgtaaga tacatgatat tatttctgaa cgaaaaagac tattaagtca
    58021 agagagagta gttaatatag agcaatataa taaagaaact ggaaattcaa ttccaaatgt
    58081 tttcttaatt atcgataact acgacacggt gaaagaatca ccatttatgg aagaatatga
    58141 agaaatgatg tctaaggtga cacgtgaagg tttagcatta ggggtataca tcgttctttc
    58201 aggatcaaga tcaagtgcaa taaaatcagc tatatttact aacattaaaa caagagttgc
    58261 gctgtacctc tttgaaaata atgaattaac aaatattatt ggttcttata aaaaaggtgt
    58321 gaaagatgtt aaaggtagag ctgcaataaa tgatgataac ttcacacagt tccaaattgc
    58381 gcaaccattt gaattagcag aaggacaaac atataacgag cgcattaaaa atgaagtagc
    58441 acaaatgaaa gaattctatg taggagatta tccaaaacat atcccaatga tgccagataa
    58501 ggtattaatg gaggatatac aagaaacata tgatttggaa aaaatcattc atgaagaaca
    58561 caaattacca ttaggattag actttgagga tgtggaacta gttagcttag atctgacatt
    58621 accaagtata ttaacagcaa gaacaccaaa tgatttagaa atagttaata atcgagtttt
    58681 agatggtatg agtaaactta aggaaagtgt aacaacaata ctagttgatg cagatgaaaa
    58741 tatgagtgat aaggcaagta tagttaattc atattatgca tcgtcagagg atttgcaatt
    58801 aataaaacaa ggttttatcg ttgaaatcaa aaaaaggttg aatagcgaac gaagtaaaga
    58861 aagtgtgaag attgtattta tcaacaatct taaggcgttt atttctatca caggacttaa
    58921 tgaaaaggat ataaggtata tattatctga aggaccaaag gtaaatgtct atacaataat
    58981 tagtagtatg tacaatgaca tcttaggaac atttgataga gaatcaaaat tagcacgtca
    59041 aattattaat caagcagtaa ttgtaactag gctatatgat caagagtttt tacaagctaa
    59101 aatcacgaat agagagccgt tattaaaacc atatgaaatg tattatttta ataaaaatga
    59161 acatattaaa ataaaattaa ttcaataggg aggacaatta tgggggaaat aaaagttgaa
    59221 acgagtagta cgataggaaa aattgaaagt atttctaatg ctggatcaaa tgtttctttt
    59281 gataatgtat cagactcgtt agaatacaca aacatttcac catttacagg atttgctgct
    59341 gctagtagta ctttaactac agctatttct aattatcatt ctattatcac tcaagacaca
    59401 gaagcaatga aaactgctgt aaatgatttt agagataagg atgctgatat agctggacaa
    59461 attgatggaa ataataattt aggagcagga ttataacata gagggagata aaaatgacta
    59521 ataaggaaga gctcaaaaaa agaaaggcac gatgtataga acaaaaacat agcaaggaaa
    59581 gtgaattgaa aagcttacaa ggagataaga aaaggctgca agatgcagtg aattctgcac
    59641 agaccataat agaagatttc aactctgaaa agaagactta tgatggtata gaaattgaaa
    59701 agaatcaatg gtctggacaa acacgtaaca aatcggatga taatcgcacc aatttaaata
    59761 cgtcaataga taaatataaa gaaaagtttg ataaggcctt aaatcaaatg aagaaggatt
    59821 tggaaaatct tgaaagtaaa atagaaggcg ttgaaacgga tatttcaaac attgctgctg
    59881 aaattcaaaa tatagacagt caattataaa tataaaagag taaaggagta taaaatgggg
    59941 aataaaataa aaatgtcaga agtgaataat tttaatgctt cattacagaa atctctcagt
    60001 agcttatcga ccaacacaaa aaccttaaag aataatatta ataaattgaa agatgatagt
    60061 gaatttaaag ggaagacagc atcgaatatt aatagttata atcaaagttt tcatattgaa
    60121 acaataagta gaatcgaaaa aataaaggag gaatttgaaa aaaaattaaa agatgcaatt
    60181 gaagattttc aaaatagtgt tgataatgac cataatgcaa ttttagatga agatgcaata
    60241 gaaaaataca aaggggaagt caaaaaatct attgaacaaa tagagagtat taagacaaga
    60301 atgaattcta ctataggagg ggttcatgat ttaacaacag caaaaacaat aagtggtaag
    60361 gatgttaaag aaaagggaga aacatttaat aagcaaatta ctgatacatt agataatttt
    60421 aataattttc aaagtttaca ctttttagat gattccgaat tattatctat gattacacct
    60481 gtttctacaa tggcctctaa agttaaaaat atgccaagta atcgatcaac tatatctgga
    60541 aatgctggat atataaaaag tgagtataaa cttcattcag aaaacaacca atttcgtgaa
    60601 ttgaaaaaag aattggaaaa atatgaaagc gcaatatatg gtggtaataa atatcgaaag
    60661 actgttaaat ctatgattga aataaaaaat gcattaattg ttgcatatat agctggtgac
    60721 ggtaatatct ttaaaggtaa tgaattatta gctaaaaacc agttggggaa attgggaaat
    60781 aaaaaattaa aattaataaa tgcaactatg caaacaaatg ttgaaaatat tgaaggaaaa
    60841 agattagtta aagctgctaa atttatactt gataaaaata ataaaatgaa attcagtgag
    60901 aaattaaaag ctggttttaa aatgaccaga aattatagcg atactgaatt caagcagcta
    60961 gaaaaagttg caggaagata caatggtaaa tctgcaatga aaagaggtgc taaagccgca
    61021 tgggaaagta tgagtcaaaa acagttacgc gaatttttga aaaatcctaa aaatttaaaa
    61081 aatatatgtt tggatgaagt taaggatttt ctaggcaaaa acaaactagg aaaatttttg
    61141 aaaagtatga gatatgctgg taagctttta ggaccactag gagcagtagc agcagtagca
    61201 aataatgttg taaatgaaaa atcaggacag aaaaaaattg ttggatctat tgttgattta
    61261 ggggcaattg gtggttcagc tgcaactggt acggcaatcg gtgctggaat tggaggacct
    61321 tttggtgcag caataggtgc tgcaattgga acaatggtag gtatttcaac agacattaag
    61381 attgttggtg gcaagtcatt gactgatctt gctaaagata aacttaataa tacggttagt
    61441 tctattagaa gtaatggttt tggtaaaacg ttaagcaatg tattttaaaa ggagatttaa
    61501 aatgaataat actaaggaaa atattgatac tcttcgaaaa ccaggtgcac aagcattgag
    61561 tttagcatca ttatttatga tacttttttc atgtctaact ttcttttatg gattagatta
    61621 tgaaaggttt ccaaattatt taaagataac gacaattata gaattaataa ttatcataat
    61681 tagtttactt caatggatta gatttataga tttcgaaaag gaaagcgcac agaaatataa
    61741 aaaaatatat gcccgatttt tagttgttat aaatgtgcta actactatca ccgcagtatt
    61801 tgcaacatgt aatctttact attttgttgc tgtacaaaat cattatgacc tattcaatta
    61861 ttggttgatg ggtacgattt caatcataat tagttattta ttattagtaa ttggcggaat
    61921 gttcacgtta ttaaaattac ctaaagtaac aaaacgatgg ggtggtaaaa ctaaaacaca
    61981 ttttggttta ttattaactg cgttgagcgc atttatatat attgaaagaa ttatcgaata
    62041 tatattagtt cctaatgtcg tagaatcgaa gtttgtcata atggtaagta tcattattat
    62101 tgcttgtaca caatttgtgg catttcaatt tattatgcaa tacagtagat tctatatttt
    62161 tgaattaaac acagcagatg atgactaaat gatatagatt tacaaaacac acttttaaaa
    62221 agtgaattga tctgaaaaag tgatttgaaa atcggaaatt ctatatagtt tttcactgct
    62281 tttatatttt aaattggagg aataataaat ggaacttgat gcattagtaa tgcctaactt
    62341 agctaaaatg gaattttcta ttaatccaga atattggaaa gacgaaatga gcagaattaa
    62401 atcatttttt atagacaaac gtatctatac agttggacca atagtattta cacgagaagt
    62461 tgtcgggcta gatgaaatga agtttgtaac ctatatttca ttaaattctg aaattgacga
    62521 tatacctgaa ttgaatatta aatttatacc ttcaattgcc cttttaccaa ctatttctca
    62581 taaatgcttt gatgaagatg aatttgaaaa tgtttatatg aaaatcaaag agtttgcagt
    62641 agaaaataac ataacgctat caaattctcc attctaccat gttatggttg attattttgg
    62701 tggtcaagta tatgaaattc acgctgaaat caatttagat gaggatgaat tggaatgaat
    62761 gaattaagct attctactat aatgtttaga aatgttatat caaaagaata tttagatata
    62821 gacttaaaaa atttaaatga agtgacaaca cattttttaa atgaagtaaa gcattatgat
    62881 ttaaatgaaa atggaccatt aattctcact attaaccagt tgatgaaaaa tgagaaagta
    62941 aatattacaa tttattcatc tattaataat ataagtagtt tacatgattc ttttgaattt
    63001 caatcttacc tttatattga tcaaatgtta cataatcaac ttagaccact aaattatgaa
    63061 gaagatatta agaagggttt agaagatata gaaagatttg caaaagaaaa tcaacttcaa
    63121 cgtatatcac catattattt tatactcaat gatgtgaatg gatttaaatg gatcgatatt
    63181 aaagtaaaag ttatggaata ttgaggtgcg aatatggagt tcttattatt aattgtcgta
    63241 gccggactgt attatattat atatttaact gctgtgatgt attctgaaaa aatagtagta
    63301 ttgcctataa tcatctatgc cattgtgttt gtagtaattg gtatcactta tatttttata
    63361 ggtgacagct atgatcagtt aacaaatttc aatgtgattt tgtatatggg gagtttgttt
    63421 tatgcatgga tggcttttag aaatctttgg aacagaccat tattattaaa atataagaac
    63481 attacagata gttcaagtgg aatagttaat aaatctgaat ataattcagt tgaaagctta
    63541 cgtataaaca ttgaaatagc taagtataaa gggattattt ctttgatagt agctatagta
    63601 ctaacggtat taatgacatt aaagtcaaca cctcaaatca ctgcagaaac acgtgactta
    63661 agtatctcat ttttcatact cagcttattt atcattataa tatttgcagt ttgggatttg
    63721 attattagag ttagaaaggg aacgtttgct tttgttgtaa taaggccaat attatttagt
    63781 tgctggttat ttattttgaa tatgatttta tcaagattat tataatataa gaagttgaat
    63841 aaattgtaat gtgaaaaatc attgagaatt tcaattattg taacaatagt ttcagttatt
    63901 ttagtttcat tagtttattt aggtttaaaa tgagtaatat tattttgaag aagcattaat
    63961 ccacgtagaa gtgttgttta atgcttttta tttaacggta atgaaaatat agggggataa
    64021 catgactttc gaagaaaaac taagtcaaat gtacaacgag attgcgaatg agatcaatgg
    64081 aatgatacca gtagactggg aaaaggtata tgcaatggca tatatagatg atggaggagg
    64141 agaagtgttc tactattaca cagaacctgg aaggaatgaa ttatactact atactagtgt
    64201 attaaataaa tatgatatat cagaatcaga atttatggac tcagagtatg agttgtataa
    64261 acaatttcaa aagttaagaa atatatttaa agaagaagga catgaaccat ggacatcatg
    64321 cgaatttgat tttacaagag aaggtaaatt aaaagtttca tttgattata tagattggat
    64381 aaattcagaa tttggtcaaa taggtcgaca aaattactat aagtatagaa aatttggaat
    64441 tttaccagaa acggaatatg aaattaataa agttaaagaa atcgagcaat atgttaaaga
    64501 acaagaagaa gctgaaatat aggggagata acatgaattt cgaagaaaaa ctaagtcaaa
    64561 agtacaacga gattgcgaat aaaattagta gcatgatacc agtagagtgg gaaaaggtat
    64621 atgcaatggc ttatataaat gaaagaaatg gagaagtttt ctacaattat actgagccaa
    64681 gcagtgatga attgttttac tatacgagcg tgttaaataa atataatata ccaagatcag
    64741 aatttatgga ctcagtatat gaattatata agcaatttga taatttaaga gaattgttta
    64801 tagaagaagg actcgagcca tggacatcat gcgaatttga ctttacaaga gagggtaaat
    64861 taaacgtatc ttttgattat attgattgga ctaaattaga atttggtcaa atagcaaaag
    64921 aaaattatta tatgtataaa aaatttggag ttatgccaga aatgaaatat gaaattaata
    64981 aagttaaaga agtagagaag tgtattaaag agcaagaaga atagactatc ttcatgtgag
    65041 actaaacaat aaagctttgt ttagtctttt ttggcggtta agtaaaaagc aatagatacc
    65101 gtaaagttga tgctcatcaa ataataatat aaagataatt ttaggttttt aaacttttaa
    65161 tcgaaaagaa tctaagtatt tagctttaaa tacgtaatat gctatttaaa aaataatgta
    65221 taggagatag atatgactac taaggaaaat atagacattc ttcgaaagcc aggtgcacaa
    65281 gccttaagtt taatatcatt gtttttgata cttttttcat gtctgacttt cttttttggt
    65341 ttagattatg aaagttttcc taattattta aagataacga caattataga attaataatt
    65401 attgtaatta gt
//
LOCUS       DAPIDM010000004        57435 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000004 DAPIDM010000000
VERSION     DAPIDM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 57435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.004"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            79..231
                     /locus_tag="RJE42_000196"
     CDS             79..231
                     /locus_tag="RJE42_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV5875655.1"
                     /translation="MVKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAASDFEKI
                     RAEVSW"
     gene            299..499
                     /locus_tag="RJE42_000197"
     CDS             299..499
                     /locus_tag="RJE42_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDV5875656.1"
                     /translation="MWIVISIVLSIFLLILLSSISHKMKTIEALEYMNAYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKL"
     gene            483..803
                     /locus_tag="RJE42_000198"
                     /pseudo
     CDS             483..803
                     /locus_tag="RJE42_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            816..1253
                     /locus_tag="RJE42_000199"
     CDS             816..1253
                     /locus_tag="RJE42_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV5875657.1"
                     /translation="MGKASYDIKPGTFKYIESEIYNLQENKKEINRLRMEILNPTKEL
                     DTNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKDKKLKIEQIGDACHMHRNTVTTIRKNFVKAIAYHAGIK"
     gene            1410..1724
                     /locus_tag="RJE42_000200"
     CDS             1410..1724
                     /locus_tag="RJE42_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV5875658.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNYLCQMC
                     LREDVVTDANLVHHIIYVDEDFNKALDLDNLMSVCYSCHNKIHANDNDKSNIKRIRVL
                     KI"
     gene            1852..2157
                     /locus_tag="RJE42_000201"
     CDS             1852..2157
                     /locus_tag="RJE42_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDV5875659.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            2147..3838
                     /locus_tag="RJE42_000202"
     CDS             2147..3838
                     /locus_tag="RJE42_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDV5875660.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDSGEGNIEFISIKDIMR"
     gene            3843..5081
                     /locus_tag="RJE42_000203"
     CDS             3843..5081
                     /locus_tag="RJE42_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV5875661.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSTSKLYDFSPWKNRSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIIKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            5065..5838
                     /locus_tag="RJE42_000204"
     CDS             5065..5838
                     /locus_tag="RJE42_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV5875662.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLDKTDAVSNSAYLDKAKNLDQEQLKQ
                     MLDAETWLTAEEALSFGLIDEVLGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMSY"
     gene            5850..7013
                     /locus_tag="RJE42_000205"
     CDS             5850..7013
                     /locus_tag="RJE42_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDV5875663.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDTGEAYQSLNDHEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKLKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGSLPS"
     gene            7082..7360
                     /locus_tag="RJE42_000206"
     CDS             7082..7360
                     /locus_tag="RJE42_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDV5875664.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            7372..7704
                     /locus_tag="RJE42_000207"
     CDS             7372..7704
                     /locus_tag="RJE42_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875665.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            7701..8102
                     /locus_tag="RJE42_000208"
     CDS             7701..8102
                     /locus_tag="RJE42_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875666.1"
                     /translation="MSVEIKGIPEVLKKLESVYGKQAMQAKSDRALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            8103..8498
                     /locus_tag="RJE42_000209"
     CDS             8103..8498
                     /locus_tag="RJE42_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDV5875667.1"
                     /translation="MNILNTIKGILLSDAELQTYINSRIYYYKVTENAETSKPFVVIT
                     PIYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            8533..9174
                     /locus_tag="RJE42_000210"
     CDS             8533..9174
                     /locus_tag="RJE42_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV5875668.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSEDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            9265..9720
                     /locus_tag="RJE42_000211"
     CDS             9265..9720
                     /locus_tag="RJE42_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDV5875669.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITATSTDGSDKSGQISVTVTNG"
     gene            9778..10128
                     /locus_tag="RJE42_000212"
     CDS             9778..10128
                     /locus_tag="RJE42_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875670.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALKDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            10170..10328
                     /locus_tag="RJE42_000213"
     CDS             10170..10328
                     /locus_tag="RJE42_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875671.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            10342..16542
                     /locus_tag="RJE42_000214"
     CDS             10342..16542
                     /locus_tag="RJE42_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV5875672.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLSLDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLAIFGASIGPAVLAGGLLIRAVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMLFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALKFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENNRIMEKVRLNSGQITEDKAKKLLKIEADLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKNDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKTKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSSWSSAKS
                     SVGYHTKALANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRSKWDNISSTAWSNAKSVWKGTSKWFGNSYKSLKGWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNSFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKRDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFDHPIWQRFGSYTGGLNFNGGRHYGIDFQMPTGTNIYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLVRQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSSLRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            16542..17366
                     /locus_tag="RJE42_000215"
     CDS             16542..17366
                     /locus_tag="RJE42_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDV5875673.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            17375..18958
                     /locus_tag="RJE42_000216"
     CDS             17375..18958
                     /locus_tag="RJE42_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV5875674.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKMPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSQTIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            18958..19248
                     /locus_tag="RJE42_000217"
     CDS             18958..19248
                     /locus_tag="RJE42_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875675.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDNLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            19264..21174
                     /locus_tag="RJE42_000218"
     CDS             19264..21174
                     /locus_tag="RJE42_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875676.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTIQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFTTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            21174..22640
                     /locus_tag="RJE42_000219"
     CDS             21174..22640
                     /locus_tag="RJE42_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDV5875677.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESESSVLLFNITK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSRRGSGANANRGTINKIIGVRK"
     gene            22640..23029
                     /locus_tag="RJE42_000220"
     CDS             22640..23029
                     /locus_tag="RJE42_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDV5875678.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQIVAFNKKLEEVKGETENA"
     gene            23022..23186
                     /locus_tag="RJE42_000221"
     CDS             23022..23186
                     /locus_tag="RJE42_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDV5875679.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYTKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENIES"
     gene            23232..23531
                     /locus_tag="RJE42_000222"
     CDS             23232..23531
                     /locus_tag="RJE42_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HDV5875680.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            23667..23969
                     /locus_tag="RJE42_000223"
     CDS             23667..23969
                     /locus_tag="RJE42_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDV5875681.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDDETISSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAENKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            23981..25435
                     /locus_tag="RJE42_000224"
     CDS             23981..25435
                     /locus_tag="RJE42_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDV5875682.1"
                     /translation="MLMTKNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGDK
                     AKNVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     SSGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEAGNYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI"
     gene            complement(26571..27398)
                     /locus_tag="RJE42_000225"
     CDS             complement(26571..27398)
                     /locus_tag="RJE42_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5875683.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSGINTEPFKMANSYYAKIAQSVNETVLAQLTS
                     PTPIIQDQIAQRFLIYGYARGTGEWINMINIADYTDSKYNISPNELKTLPPVFVAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMA"
     gene            complement(27456..28670)
                     /locus_tag="RJE42_000226"
     CDS             complement(27456..28670)
                     /locus_tag="RJE42_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDV5875684.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADTLRNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPQDIDLEFSMEDLENLAIPRAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDTSSKDNFS"
     gene            28766..28858
                     /locus_tag="RJE42_000227"
     CDS             28766..28858
                     /locus_tag="RJE42_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875685.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNTSEKI"
     gene            28972..29973
                     /locus_tag="RJE42_000228"
     CDS             28972..29973
                     /locus_tag="RJE42_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV5875686.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKITAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            30007..30339
                     /locus_tag="RJE42_000229"
     CDS             30007..30339
                     /locus_tag="RJE42_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDV5875687.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            30340..31140
                     /locus_tag="RJE42_000230"
     CDS             30340..31140
                     /locus_tag="RJE42_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDV5875688.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFKRDAE"
     gene            31130..32074
                     /locus_tag="RJE42_000231"
                     /pseudo
     CDS             31130..32074
                     /locus_tag="RJE42_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
     gene            32052..33074
                     /locus_tag="RJE42_000232"
     CDS             32052..33074
                     /locus_tag="RJE42_000232"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDV5875689.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLLNNRIYGGYSLLLDVN
                     YEAMDEVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKDLMVTQILGID
                     DIKEAKRYELSDADWEAIQELARKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            33388..34413
                     /locus_tag="RJE42_000233"
     CDS             33388..34413
                     /locus_tag="RJE42_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDV5875690.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGIPTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMKNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLSDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(34510..35853)
                     /locus_tag="RJE42_000234"
     CDS             complement(34510..35853)
                     /locus_tag="RJE42_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV5875691.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQ"
     gene            complement(35868..36152)
                     /locus_tag="RJE42_000235"
     CDS             complement(35868..36152)
                     /locus_tag="RJE42_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV5875692.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLAEKLQQLNMI"
     gene            complement(36154..36597)
                     /locus_tag="RJE42_000236"
     CDS             complement(36154..36597)
                     /locus_tag="RJE42_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV5875693.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(36602..38557)
                     /locus_tag="RJE42_000237"
     CDS             complement(36602..38557)
                     /locus_tag="RJE42_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDV5875694.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTANTTILNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDAREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAITVAVQPLINDGIVNSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEV"
     gene            38769..39188
                     /gene="mepR"
                     /locus_tag="RJE42_000238"
     CDS             38769..39188
                     /gene="mepR"
                     /locus_tag="RJE42_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDV5875695.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLMKMLSSLQ"
     gene            39295..40650
                     /gene="mepA"
                     /locus_tag="RJE42_000239"
     CDS             39295..40650
                     /gene="mepA"
                     /locus_tag="RJE42_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDV5875696.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVALPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGVVIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            40753..41193
                     /locus_tag="RJE42_000240"
     CDS             40753..41193
                     /locus_tag="RJE42_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDV5875697.1"
                     /translation="MYKSKILLKYIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(41276..42634)
                     /gene="glpT"
                     /locus_tag="RJE42_000241"
     CDS             complement(41276..42634)
                     /gene="glpT"
                     /locus_tag="RJE42_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV5875698.1"
                     /translation="MNILKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLVLT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            42946..43872
                     /locus_tag="RJE42_000242"
     CDS             42946..43872
                     /locus_tag="RJE42_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5875699.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLRREGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPKMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            43886..44947
                     /locus_tag="RJE42_000243"
     CDS             43886..44947
                     /locus_tag="RJE42_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV5875700.1"
                     /translation="MAKLEMDKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQVATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSPDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            44961..45527
                     /locus_tag="RJE42_000244"
     CDS             44961..45527
                     /locus_tag="RJE42_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDV5875701.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVLEEENNQYDF"
     gene            complement(45587..46582)
                     /locus_tag="RJE42_000245"
     CDS             complement(45587..46582)
                     /locus_tag="RJE42_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV5875702.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            47002..47547
                     /locus_tag="RJE42_000246"
     CDS             47002..47547
                     /locus_tag="RJE42_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5875703.1"
                     /translation="MFGMKVNEQITLKILEVHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQSSDTRAFIKRGLLQFADGNGFQCGIWYGGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     YEGHGIMTQAVEALIKYCFDEIDLNRIEISVAVNNEKSQAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEFNEG"
     gene            47812..48666
                     /locus_tag="RJE42_000247"
     CDS             47812..48666
                     /locus_tag="RJE42_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDV5875704.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKASKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVNAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNKLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            48663..49892
                     /gene="efeB"
                     /locus_tag="RJE42_000248"
     CDS             48663..49892
                     /gene="efeB"
                     /locus_tag="RJE42_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV5875705.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCKFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKASNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLLDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATQQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            49873..51585
                     /locus_tag="RJE42_000249"
     CDS             49873..51585
                     /locus_tag="RJE42_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDV5875706.1"
                     /translation="MKHYLTKIIVMLITAAMVCNFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDDQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTTKVSHAWTTFKSNIDHVDK
                     KSGTSANDQYHVSQLNDELEKAIKAIDNNQLSDADTALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVGLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDNKGTASVIGGAIAGLVLSIILAITFVETLGNSGMLRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNAMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFVIGIALAIVILIIFALLFRFIVRLIPIFYIFRVLSNFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIIVVAILIF
                     KFKK"
     gene            complement(51853..52553)
                     /gene="tatC"
                     /locus_tag="RJE42_000250"
                     /pseudo
     CDS             complement(51853..52553)
                     /gene="tatC"
                     /locus_tag="RJE42_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
     gene            complement(52570..52794)
                     /locus_tag="RJE42_000251"
     CDS             complement(52570..52794)
                     /locus_tag="RJE42_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDV5875707.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(52894..53283)
                     /locus_tag="RJE42_000252"
     CDS             complement(52894..53283)
                     /locus_tag="RJE42_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV5875708.1"
                     /translation="MAFTLSEIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAPSSNQSIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            53524..53727
                     /locus_tag="RJE42_000253"
     CDS             53524..53727
                     /locus_tag="RJE42_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5875709.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNEKVETIFIIEEDEA"
     gene            53724..54437
                     /locus_tag="RJE42_000254"
     CDS             53724..54437
                     /locus_tag="RJE42_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDV5875710.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     IIQTIIALLVLLIFVVGQAEANGILFFLIPFFVSGIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            54462..55304
                     /locus_tag="RJE42_000255"
     CDS             54462..55304
                     /locus_tag="RJE42_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875711.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTIATNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSILFFGEEHAMDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTSWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEEKDVEIPKHLIIASRLSKGKYQILVKDYEEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            55304..55933
                     /locus_tag="RJE42_000256"
     CDS             55304..55933
                     /locus_tag="RJE42_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDV5875712.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIFASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTGGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(56302..57435)
                     /locus_tag="RJE42_000257"
     CDS             complement(56302..57435)
                     /locus_tag="RJE42_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDV5875713.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTIQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIGTGLDYGDKRTCQAFATVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHK"
BASE COUNT        20601 a         8207 c        11443 g        17184 t
ORIGIN      
        1 agttaagacg attggatata gaatacactt tttaaattta tatccggtct tatacgaagc
       61 gaagaaggta ggtaaatgat ggttaaacaa atattaagac tattattctt actagcgatg
      121 tatgagttag gtaagtatgt aactgagcaa gtatatatta tgatgacggc taatgatgat
      181 gtagaggcag caagtgactt tgaaaaaatc agagctgaag tttcatggta atagctatta
      241 tcatttttga attaattata ttaatgtgtt tagcaatagc actggaggtg ttgtaaatat
      301 gtggattgtc atttcaattg ttttatctat atttttattg atcttgttaa gtagcatttc
      361 tcataagatg aaaaccatag aagcattgga gtatatgaat gcttatcttt tcaagcagtt
      421 agtaaaaaat aatggtgttg aaggtttaga agattatgaa aatgaagttg aacgaattag
      481 aaaaagattc aaactataaa gaacgttgga atagtggaga cattaagctg cttatagcac
      541 atccagcaag tgcagggcat ggattaaact tacaacaagg tgggcacatt attgtttggt
      601 ttggacttac atggtcattg gaattatacc aacaagcaaa tgcaagatta tatagacaag
      661 gacaaaatca tacgactatt attcatcaca tcatgaccga taacacaata gatcaaagag
      721 tatataaagc tttacaaaat aaagaactaa cgcaagaaga attgatgaaa gctattaaag
      781 caagaatagc taagcataag taatggaggt ataagatggg aaaggcatca tacgatatta
      841 agccaggtac atttaaatat attgagtcag agatatataa cctacaagag aacaagaaag
      901 agataaatag attgagaatg gagatactta acccaacgaa agaactagac accaacattg
      961 tgtatggacc gttacaaaaa ggagagccag ttagaacaac tgagttaatg gcgacaaggt
     1021 tattgactaa taagatgtta cgtaacttag aagagatggt tgaagcagtt gaaagtgagt
     1081 acttaaagtt acctgaagat cataagaaag taataaggtt aaagtattgg aataaagata
     1141 agaagctaaa gatagaacaa ataggggatg cttgtcacat gcatcgcaat acagttacta
     1201 caatacgaaa gaactttgtt aaagcgatag cgtatcatgc aggtatcaaa taacattgtg
     1261 caaagattgt gcaaaaggcc tacaaatctg tagtaatatg atagtatcgg aaagatgtat
     1321 aaagttatct gaaagttata cgacataaat acatgaggcg catcgctaag cggtgtgtct
     1381 tttgttatgc aatcaaagag gtgtaagaga tgaccaagca taataacatt tataagcatg
     1441 gtcgtaagtc atatcaatac gattggttct atcattcaaa agcatggaag aagttaagag
     1501 agattgcatt agatagagat aattatcttt gtcaaatgtg tttacgtgaa gatgttgtaa
     1561 cagatgcaaa cctagtgcat cacattattt atgttgatga agattttaac aaagctttag
     1621 acttagataa tctaatgtca gtttgttata gctgtcataa caaaattcat gcaaatgata
     1681 atgacaaaag taatattaag agaattagag ttctaaaaat ttaaacaaaa aaattattta
     1741 aataaaattt tatagccccc tgcccatcgg cttaaaatgt tttttcgccg ggtaccggcg
     1801 ggggcccttc gcttgcaacg cggataaact tttatgaaag ggggtcttta tatgaaatta
     1861 acaaaaaaac agctgaaaga atatatagag gattataaaa aatctgatga catattaatt
     1921 aatttgtata tagaaacgta tgaattttat tgtcggttaa gagatgaact taaaaatagt
     1981 gatttgatga tagagcatac aaacaaggct ggtgcgagca atattgttaa gaatccatta
     2041 agcatagaac tgacaaaaac agttcaaaca ctaaataact tactcaagtc tatgggttta
     2101 actgcagcac aaagaaaaaa gatagttcaa gaagaaggtg gattcggtga ctattaaagt
     2161 tttaaatgaa ccttcaccaa aactattaac aacatggtat gcagagcaag tcactcaagg
     2221 gaaaataaaa acaagcaaat atgttaaaaa agaatgtgag agacacctta gatatctaga
     2281 aaatggaggt aaatgggtat ttgatgaaga attagcgcac cgtcctattc gattcataga
     2341 aaagttttgt aaaccttcca aaggatctaa acgtcaactt gtattacaac catggcaaca
     2401 ttttattatt ggcagtttgt ttggttgggt tcataaagaa acaaaactgc gcaggtttaa
     2461 agaagctttg atatttatgg ggcgaaaaaa tggtaaaaca actactatat ctggtgttgc
     2521 taactatgct gtttctcaag atggagaaaa cggcgctgaa atccatcttt tagcaaacgt
     2581 aatgaaacaa gctagaatat tattcgatga atctaaggcg atgataaaag ctagcccaaa
     2641 gcttgataaa aatttcagaa cattaagaga tgaaatccat tatgacgcaa cgatatcaaa
     2701 aattatgccc caagcatcag atagcgataa gttagatgga ttgaatacac acatggggat
     2761 ttttgatgaa attcatgaat ttaaagacta taaattgatt tcagttataa aaaactcaag
     2821 agctgcaagg ttacaacctc ttctcatcta cattacgaca gcagggtatc aattagatgg
     2881 tccacttgtt gatatggtag aagcgggaag agacacctta gatcaaatca tagaagacga
     2941 aagaactttt tattatttag catctttgga tgatgacgat gatattaatg attcgtcgaa
     3001 ctggataaaa gcaaatccca acttaggtgt ctctataaat ttagatgaga tgaaagaaga
     3061 gtgggaaaaa gctaagagaa caccagctga acgtggagat tttataacca aaaggtttaa
     3121 tatctttgct aataatgacg agatgagttt tattgattac ccaacactcc aaaaaaataa
     3181 tgaaattgtt tctttagaag agctggaagg cagaccgtgc acgattggtt atgatttatc
     3241 agaaacagag gactttacag ccgcgtgtgc tacttttgcg ttagataatg gtaaagttgc
     3301 agttttatcg cattcatgga ttcctaagca caaagttgaa tattctaacg aaaaaatacc
     3361 ctatagagaa tgggaagaag atggcttatt aacagtgcaa gataagcctt atattgacta
     3421 ccaagatgtt ttaaattgga taattaagat gaatgagcat tatgtagtag aaaaaatcac
     3481 ttatgataga gcgaacgcat tcaaactaaa tcaagagtta aaaaattacg ggtttgaaac
     3541 ggaagaaaca agacaaggag ctttgacctt gagccctgca ttgaaggatt taaaagaaat
     3601 gtttttagat gggaaaataa tatttaataa taatccttta atgaaatggt atatcaataa
     3661 tgttcagttg aaactagaca gaaacggaaa ctggttgccg tctaagcaaa gcagatatcg
     3721 taaaatagat ggctttgcag catttttaaa cacatataca gatattatga ataaagttgt
     3781 ttctgatagt ggtgaaggaa acatagagtt tattagtatt aaagacataa tgcgttaagg
     3841 aggtgaatgt tatcgcaaaa gagaatattg tcacacgcat aaagaaaaaa ttgatagaca
     3901 attggattga tcagtcaact tctaagcttt atgactttag cccatggaaa aatagatctt
     3961 tttggggtgt aattaataat acgcttgaaa ctaatgaaac gatattttca gctattacaa
     4021 agttatctaa ttcgatggct agtttgccct tgaaaatgta tgaagattat aaagtagtta
     4081 atacagaagt atctgattta cttacagtgt caccgaataa ttctctgagc agttttgatt
     4141 ttattaatca aattgaaaca atcagaaatg aaaaaggtaa tgcatatgtg ctaattgaac
     4201 gagacatcta tcatcaacca tcaaagcttt tcttattaaa tccagatgtt gttgaaatgt
     4261 taattgaaaa ccaatcacgt gaactttatt attccattca tgctgcaact ggaaataaat
     4321 tgattgttca taatatggac atgttgcatt ttaaacacat cgtggcatct aatatggtgc
     4381 aaggcattag tccgattgat gtgttgaaga atacaactga ttttgataat gcagtaagaa
     4441 cctttaatct tacagaaatg caaaaacctg attctttcat gcttaaatat ggttccaatg
     4501 taggtaaaga aaaaaggcag caagtgttag aagatttcaa acagtactat gaagaaaacg
     4561 gtggaatatt attccaagag cctggtgttg aaatcgaacc gttacctaaa aaatatgtct
     4621 ctgaagatat agtggcaagc gagaatttaa caagagaaag agtagctaac gtttttcaat
     4681 tgccctcagt attcttaaat gcaagatcaa atacaaattt cgcgaaaaat gaagagttaa
     4741 acagatttta cttgcagcat accttattgc cgattatcaa acagtacgaa gaagaattta
     4801 atcggaaact acttactaaa acagacagag aaaaaaatag gtattttaaa tttaacgtta
     4861 aatcttattt aagggctgat agtgcaacac aagcagaagt gtactttaaa gcagttcgta
     4921 gtggttacta cactataaat gacattagag agtgggaaga tttaccacca gttgaaggtg
     4981 gagataagcc gctaataagc ggtgatttat acccaattga cacgccactt gaattaagaa
     5041 aatctttgaa aggtggtgat aaaaatgtca atgaaagcta agtattttca aatgaaaaga
     5101 aaatcaaaaa gtaaaggtga aatatttatt tatggtgata ttgtaagtga taaatggttt
     5161 gaaagtgatg taactgctac agatttcaaa aataaactag atgaactagg agacatcagt
     5221 gaaatagatg ttcatataaa ttcatctgga ggcagtgtat ttgaagggca tgcaatatac
     5281 aatatgctaa aaatgcatcc tgcaaaaatt aatatctatg tcgatgcctt agcggcatca
     5341 attgctagtg ttatcgctat gagtggtgac actattttta tgcacaaaaa tagtttttta
     5401 atgattcata attcatgggt tatgactgta ggtaatgcag aagaattaag aaagacagcg
     5461 gatttacttg ataaaacaga tgctgttagt aattcagcgt atttggataa agcaaaaaac
     5521 ttagatcaag aacaattaaa gcagatgtta gatgcagaaa catggcttac tgctgaagaa
     5581 gccttatctt ttggcttaat agatgaagtt ttaggagcta atgaaatagc tgctagtatc
     5641 tctaaagagc agtataagcg tttcgagaac gtcccggaag atttaaagaa agatgtagac
     5701 aaaatcacaa aaattgatga tgtagataca tctgaattgg ttgaaacacc taaagaaagt
     5761 atgtcactag aagaaaaaga aaaaagagaa aaaattaaac gcgaatgcga aattttaaaa
     5821 atgacaatga gttattagga ggaaatgaaa tgccgacatt atatgaatta aaacaatcat
     5881 taggtatgat tggacaacaa ttaaaaaata aaaatgatga gttgagtcag aaagcaacag
     5941 atccaaatat tgatatggaa gacatcaaac aactagaaac agaaaaagca ggcttacaac
     6001 aaagatttaa cattgttgaa agacaagtac aagacattga agaaaaagaa aaagcgaaag
     6061 ttaaagacac aggagaagct tatcaatctt taaatgatca tgagaagatg gttaaagcta
     6121 aggcagagtt ttatcgtcac gcgattttac caaatgaatt tgaaaaacct tcaatggagg
     6181 cacaacgttt attacacgct ttaccaacag gtaatgattc aggtggagat aagctcttac
     6241 caaaaacact ttctaaagaa attgtttcag aaccatttgc taaaaaccaa ttacgtgaaa
     6301 aagctcgtct aactaacatt aaaggtttag agattccaag agtttcatac actttagacg
     6361 atgatgattt cattacagac gtagaaacag caaaagaatt aaaattaaaa ggtgatacag
     6421 tcaagttcac tactaataaa ttcaaagtat ttgctgcaat ttcagatact gtaattcatg
     6481 gatcagatgt agatttagta aactgggttg aaaacgcact acaatcagga ttagcagcta
     6541 aagagcgtaa agatgcctta gcagtaagtc ctaaatctgg attagaacac atgtcatttt
     6601 ataatggatc tgttaaagaa gttgagggag cagacatgta tgatgctatt attaacgctt
     6661 tagcagattt acatgaagat taccgtgata acgcaacaat ttatatgcga tatgcagatt
     6721 atgtcaaaat tattagtgtt ctttcaaatg gaacaacaaa tttctttgac acaccagcag
     6781 aaaaagtatt tggcaaacca gtagtattta cagatgcagc agttaaacct attgtgggag
     6841 atttcaatta ttttggaatt aactatgatg gaacaactta tgacactgat aaagatgtta
     6901 aaaaaggcga atatttgttt gtattaacag catggtatga tcagcaacgt acattagaca
     6961 gtgcattcag aattgcaaaa gcaaaagaaa atacaggttc attacccagc taaaccccaa
     7021 aaggttaatg taacagctaa ggctaaatca gctgtaatat cagccgaata ggggtgatga
     7081 aatgagtttg gaagaaatta aattgtggtt gagaattgac tataatttcg aaaatgattt
     7141 aattgaaggt ctcattcaat cggctaagtc tgaattatta ttaagtgggg ttccagatta
     7201 tgacaaagat gacttggaat acccgctttt ttgtacagcg attaaatata tcattgcaag
     7261 agattatgaa agtcgtggat actcaaatga ccaatctaga agcaaggtgt ttaatgaaaa
     7321 aggattgcaa aaaatgattt tgaaattaaa aaagtggtag gtgattttta aatggaattt
     7381 aatgaattta aagatcgcgc gtattttttt caatatataa acaaaggacc atatccagat
     7441 gaagaggaaa aaatgaaatt gtatagttgc ttttgtaaaa tttataatcc ttctatgaaa
     7501 gatagagaaa ttttaaaagc gactgaatca aaatcaggac taaccataat tgtcaggtct
     7561 tctaaaactg aatatctacc acaaacaaat cacttagtta aaattgacag tgcattatat
     7621 tccgataaat tattcaacat tgtagaaata agaattgata caccagatat aggctataat
     7681 acagtggttt tatcagaaaa atgagtgtag aaattaaagg aatacctgaa gtgttgaaga
     7741 aattagaatc ggtatacgga aagcaagcaa tgcaagctaa gagtgataga gctttaaatg
     7801 aagcatccga attttttata aaggctttaa agaaagagtt cgagagcttt aaagatacgg
     7861 gtgccagtat agaagaaatg actaaatcta agccttatac aaaagttggc agtcaagaaa
     7921 gggctgtttt aattgaatgg gtaggcccta tgaatcgcaa aaacattatt cacttgaatg
     7981 aacatggtta tacaagagat ggaaaaaaat atacaccaag aggttttgga gttattgcaa
     8041 aaacattagc tgctagcgaa cgtaagtata gagaaattat aaaaaaggag ttggccagat
     8101 aaatgaatat attaaacacc ataaaaggaa ttttattatc tgatgcagag ctccaaacat
     8161 atataaattc tagaatatac tattataaag tcactgaaaa tgctgaaact tccaaacctt
     8221 ttgttgttat tacacctatt tatgatttac cttcagactt tatgtctgat aaatatctta
     8281 gtgaagaata cttaattcaa atagatgtag aatcttcaaa taatcagaaa acaattgata
     8341 taacaaaacg aataagatac ctgttatatc aacaaaattt aattcaagca tctagtcagt
     8401 tagatgctta ttttgaagaa actaaacgtt atgtgatgtc gagacgttat caaggcatac
     8461 caaaaaatat atattataaa aatcagcgca tcgaataggt gtgcttttta atttttaagg
     8521 aggaaataag caatggcaga aggacaaggt tcttataaag taggttttaa aagattatac
     8581 gttggagttt ttaacccaga agcaacaaaa gtagttaaac gcatgacatg ggaagatgaa
     8641 aaaggtggta cagttgatct aaatatcaca ggtttagcac cagatttagt agatatgttt
     8701 gcatctaaca aacgtgtttg gatgaaaaaa caaggtacta atgaagttaa gtctgacatg
     8761 agtattttta acatcccaag tgaagattta aatacagtta ttggtcgtac taaagataaa
     8821 aatggtacat cttgggtagg agagaataca agagcaccat acgtaacagt tattggagaa
     8881 tctgaagatg gtttaacagg tcaaccagtg tacgttgcgc tacttaaagg tacttttagc
     8941 ttggattcaa ttgaatttaa aacacgaggt gaaaaagcag aagccccaga acctacaaaa
     9001 ttaacaggtg actggatgaa tagaaaagtt gatgttgatg gaacgtcaca aggtattgta
     9061 tacggttatc atgaaggtaa agaaggagaa gcagaattct tcaaaaaagt attcgttgga
     9121 tacacggaca gtgaagatca ttcagaggat tctgcaggtt cgttacccag ctaaccccca
     9181 aaatgttgaa gtagcagtta attcaaaatc tgcaacagtt tcagcagaat aggggctttc
     9241 aaataaatca aaggagaata atttatgact aaaactttaa aggtttataa aggagacgac
     9301 gtcgtagctt ctgaacaagg tgaaggcaaa gtgtcagtaa ctttatctaa tttagaagcg
     9361 gatacaactt atccaaaagg tacttaccaa gtggcatggg aagaaaatgg taaagaatct
     9421 agtaaagttg atgtacctca attcaaaacc aatccaattc tagtctcagg cgtatcattt
     9481 acaccagaaa ctaaatcaat tatggtaaat accgatgaca atgttgagcc aaacattgca
     9541 ccaagcacag caacgaataa aatattgaaa tatacaagtg aacatccaga atttgttact
     9601 gtagatgaaa atacaggagc aattcacggt gtagctgaag gtacttcagt aatcactgct
     9661 acgtctactg atggaagcga taagtcagga caaatttcag tgacagtaac aaacggatag
     9721 gaatttaagg cgcagtatat ctgcgtcttt tttatttgaa taaaaggagc taatacaatg
     9781 attaaatttg aaattaaaga tcgtaaaaca ggaaaaacag agagctatac aaaagaagat
     9841 gtaacaatgg gcgaagcaga aaaatgctat gagtatttag aattagtaaa tcaagagaat
     9901 aaaaaagaag cacctaacgc aacaaaaatg agacaaaaag agcgacagtt attagtagat
     9961 ttatttaaag atgaaggatt gactgaagaa gatgttttga acaagatgag cactaaaact
    10021 tatacaaaag ccttgaaaga tatatttcga gaaatcaatg gtgaagatga agaagattca
    10081 gaaactgaac cagaagagat gggaaagaca gaagaacaat ctcaataaaa gacattttat
    10141 cgaacattaa gaaaatacaa cgtttctgta tggagcagta tgggtggaca ttaactgaag
    10201 tcagaaaaca gccgtatgta aaacttttag aaatacttaa tgaagaaaat aaagaagaga
    10261 ctgaagaaaa acaaagtgaa caaaaagtca ttacaggtac ggatttaaga aaactttttg
    10321 gaagctagaa aggaggttaa tatgaatgaa aaagtagaag gcatgacctt ggagctgaaa
    10381 ttagaccatt taggtgtcca agaaggcatg aaaggtttaa agcgacaatt aggtgttgtt
    10441 aatagtgaaa tgaaagctaa tctgtcagca tttgataagt ctgaaaaatc aatggaaaaa
    10501 tatcaggcga gaattaaggg gttaaatgat aggcttaaag ttcaaaaaaa gatgtattct
    10561 caagtagaag atgagcttaa acaagttaac gctaattacc aaaaagctaa atccagtgta
    10621 aaagatgttg agaaagcata tttaaagtta gtagaagcta ataaaaaaga aaaattatct
    10681 cttgataaat ctaaagaagc cttaaaatca tcaaatacag aactcaaaaa agctgaaaat
    10741 caatataaac gtacaaatca acgtaaacaa gatgcgtatc aaaaacttaa acagttgaga
    10801 gatgcagaac aaaagcttaa gaatagtaac caagctacta ctgcacaact aaaaagagca
    10861 agtgacgcag tacagaagca gtccgctaag cataaagcac ttgttgaaca atataaacaa
    10921 gaaggcaatc aagttcaaaa actaaaagtg caaaatgaca atctttcaaa atcaaatgat
    10981 aaaattgaaa gttcttacgc taaaactaat actaaattaa agcaaacaga aaaagaattt
    11041 aatgatttaa acaatactat taagaatcat agcgctaatg tcgcaaaagc tgaaacagct
    11101 gttaataaag aaaaagctgc tttaaataat ttggagcgtt caatagataa agcttcatcc
    11161 gaaatgaaga cttttaacaa agaacaaatg atagctcaaa gtcatttcgg taaacttgca
    11221 agtcaagcgg atgtcatgtc aaagaaattt agttctattg gagacaaaat gacttccctg
    11281 ggacgtacaa tgacgatggg cgtatctaca ccaattactt tagggttagg tgcagcatta
    11341 aaaacaagtg cagactttga aggccaaatg tctcgagttg gagcgattgc gcaagcaagc
    11401 agtaaagact tgaaaagcat gtctaatcaa gcagttgact taggagctaa aacaagtaaa
    11461 agtgctaacg aagttgctaa aggtatggaa gaattggcag ctttaggctt taatgccaaa
    11521 caaacaatgg aggctatgcc aggtgttatc agtgcagcag aagcaagtgg tgcagaaatg
    11581 gctacaactg caactgtaat ggcttcagcg attaactctt tcggtttaaa agcatctgat
    11641 gcaaatcatg ttgctgattt acttgcgaga tcagcaaatg atagtgctgc agatattcag
    11701 tacatgggag atgcattgaa gtatgctggt actccagcaa aagcattagg agtttcaata
    11761 gaggacactt ccgcagcaat tgaagtttta tctaactcag gtttagaggg ttctcaagca
    11821 ggtactgccc taagagcttc atttatcagg ctagctaatc caagtaaaaa tacagctaag
    11881 gaaatgaaaa aattaggtat tcatttgtct gatgctaaag gtcaatttgt tggcatgggt
    11941 gaattgatta gacagttcca agataatatg aaaggcatga cgagagaaca aaaactagct
    12001 acagtggcta caatagttgg tactgaagca gcaagtggat ttttagcctt gattgaagcg
    12061 ggaccagata aaattaatag ctatagtaaa tccttaaaga attccaatgg cgaaagtaaa
    12121 aaagcagctg atttgatgaa agacaacctc aaaggtgctc tggaacaatt aggtggcgct
    12181 tttgaatcgt tagcaattga agttggtaaa gatttaacgc ctatgattag agcaggtgcg
    12241 gaaggattaa caaaattagt tgatggattt acacatcttc ctggttgggt tagaaaggct
    12301 tcggtaggtt tagcgatttt tggtgcatct attggccctg ctgttcttgc tggtggctta
    12361 ttaatacgtg cagttggaag cgcggctaaa ggctatgcat cattaaatag acgcattgct
    12421 gaaaatacaa tactgtctaa taccaattca aaagcaatga aatctttagg tcttcaaacc
    12481 ttatttcttg gttctacaac aggaaaaacg tcaaaaggct ttaaaggatt agccggagct
    12541 atgttgttta atttaaaacc tataaatgtt ttgaaaaatt ctgcaaagct agcaatttta
    12601 ccgttcaaac ttttgaaaaa cggtttagga ttagccgcaa aatccttatt tgcagtaagt
    12661 ggaggcgcaa gatttgctgg tgtagcctta aagtttttaa caggacctat aggtgctaca
    12721 ataactgcta ttacaattgc atataaagtt tttaaaactg catatgatcg tgtggaatgg
    12781 ttcagaaacg gtattaacgg tttaggagaa actataaagt tttttggtgg caaaattatt
    12841 ggcggtgctg ttaggaagct aggagagttt aaaaattatc ttggaagtat aggcaaaagc
    12901 ttcaaagaaa agttttcaaa ggatatgaaa gatggttata aatctttgag tgacgatgac
    12961 cttctgaaag taggagtcaa caagtttaaa ggatttatgc aaaccatggg cacagcttct
    13021 aaaaaagcat ctgatactgt aaaagtgttg gggaaaggtg tttcaaaaga aacagaaaaa
    13081 gctttagaaa aatacgtaca ctattctgaa gagaacaaca gaatcatgga aaaagtacgt
    13141 ttaaactcgg gtcaaataac agaagacaaa gcaaaaaaac ttttgaaaat tgaagcggat
    13201 ttatctaata accttatagc tgaaatagaa aaaagaaata aaaaggaact cgaaaaaact
    13261 caagaactta ttgataagta tagtgcgttc gatgaacaag aaaagcaaaa cattttaact
    13321 agaactaaag aaaaaaatga cttgcgaatt aaaaaagagc aagaactcaa tcagaaaatc
    13381 aaagaattaa aagaaaaagc tttaagtgat ggtcagattt cagaaaatga aagaaaagaa
    13441 attgaaaagc ttgaaaatca aagacgtgac atcactgtta aagaactgag taagactgaa
    13501 aaagagcaag agcgtatttt agtaagaatg caaagaaaca gaaatgctta ttcaatagac
    13561 gaagcgagca aagcaattaa agaagcagaa aaagcaagaa aagcaagaaa aaaagaagtg
    13621 gacaagcaat atgaagatga tgtcattgct ataaaaaata acgtcaacct ttctaagtct
    13681 gaaaaagata aattattagc tattgctgat caaagacata aggatgaagt aagaaaggca
    13741 aaatctaaaa aagatgctgt agtagacgtt gttaaaaagc aaaataaaga tattgataaa
    13801 gagatggatt tatccagtgg tcgtgtatat aaaaatactg aaaagtggtg gaatggcctt
    13861 aaaagttggt ggtctaactt tagagaagac caaaagaaga aaaatgataa gtacgctaaa
    13921 gaacaagaag aaacagctcg tagaaacaga gaaaatataa agaaatggtt tggaaatgct
    13981 tgggacggcg taaaaactaa aactggcgaa gcttttagta aaatgggcag aaatgctaat
    14041 cattttggcg gcgaaatgaa aaaaatgtgg agtggaatca aaggaattcc aagcaaatta
    14101 agttcaagtt ggagctcagc caaaagttct gtaggatatc acactaaggc tttagctaat
    14161 agtactggta aatggtttgg aaaagcttgg caatctgtta aatcgacaac aggaagtatt
    14221 tacaatcaaa ctaagcaaaa gtattcagat gcctcagata aagcttgggc gcattcaaaa
    14281 tctatttgga gaggcacatc aaaatggttt agcaatgcat ataaaagtgc aaagggctgg
    14341 ctaacggata tggctaataa atcacgctcg aaatgggata atatttctag tacagcatgg
    14401 tcgaatgcaa aatccgtttg gaaaggcaca tcgaaatggt ttggtaactc atacaaatct
    14461 ttaaaaggtt ggactgggga tatgtattca agagcccacg atcgttttga tgcaatttca
    14521 agttcggcat ggtctaacgc taaatcagta tttaatagtt ttagaaaatg gctatcaaaa
    14581 acatatgatt ggattagaga tattggtaaa gacatgggaa gagctgcggc tgatttaggt
    14641 aaaaatgttg ctaataaagc tattggcggt ttgaatagca tgattggcgg tattaataaa
    14701 atatctaaag ccattactga taaaaatctc atcaagccaa tacctacatt gtctactggt
    14761 actttagcag gaaagggtgt agctaccgat aattcgggag cattaacgca accgacattt
    14821 gctgtattaa atgatagagg ttctggaaac gccccaggtg gtggagttca agaagtaatt
    14881 cacagggctg acggaacatt ccatgcaccc caaggacgag atgtggttgt tccactagga
    14941 gttggggata gcgtaataaa tgctaatgac actctgaagt tacagcgtat gggtgttttg
    15001 ccaaaattcc atggtggtac gaaaaagaga gattggctag accaactaaa aggtaatata
    15061 ggtaaaaaag cgggagaatt tggagctaca gctaaaaaca cagcgcataa tatcaaaaaa
    15121 ggtgcagaag aaatggttga agcagcaggc gataaaatca aagatggtgc atcttggtta
    15181 ggcgataaaa tcggcgatgt gtgggattat gtacaacatc cagggaaact agtaaataaa
    15241 gtaatgtcag gtttaaatat taattttgga ggcggagcta acgctacagt aaaaattgct
    15301 aaaggcgcgt actcattgct caaaaagaaa ttagtagaca aagtaaaatc gtggtttgaa
    15361 gattttggtg gtggaggcga tggaagctat ctatttgacc atccaatttg gcaaaggttt
    15421 gggagctaca caggtggact taactttaat ggcggtcgtc actatggtat cgactttcaa
    15481 atgcctactg gaacgaacat ttatgctgtt aaaggtggta tagctgataa agtatggact
    15541 gattacggtg gcggtaattc tatacaaatt aagaccggtg ctaacgaatg gaactggtat
    15601 atgcatttat ctaagcaatt agtaagacaa ggccaacgta ttaaagctgg tcaactgata
    15661 gggaaatcag gtgctacagg taatttcgtt agaggagcac acttacattt ccaattgatg
    15721 caagggtcgc atccagggaa tgatacagct aaagatccag aaaaatggtt gaagtcactt
    15781 aaaggtagtg gcgttcgaag tggttcaggt gttaataagg ctgcatctgc ttgggcaggc
    15841 gatatacgtc gtgcagcaaa acgaatgggt gttaatgtta cttcgggtga tgtaggaaat
    15901 atcattagct tgattcaaca cgaatcagga ggaaatgcag gtataactca atctagttcg
    15961 cttagagaca tcaacgtttt acagggcaat ccagcaaaag gattgcttca atatatccca
    16021 caaacattta gacattatgc tgttagaggt cacaacaata tatatagtgg ttacgatcag
    16081 ttattagcgt tctttaacaa cagatattgg cgctcacagt ttaacccaag aggtggttgg
    16141 tctccaagcg gtccaagaag atatgcgaat ggtggtttga ttacaaagca tcaacttgct
    16201 gaagtgggtg aaggagataa acaggagatg gttatccctt taactagacg taaacgagca
    16261 attcaattaa ctgaacaggt tatgcgcatc atcggtatgg atggcaagcc aaataacatc
    16321 actgtaaata atgatacttc tacagttgag aaattgttga aacaaattgt tatgttaagt
    16381 gataaaggaa ataaattaac agatgcattg attcaaactg tttcttctca ggataataac
    16441 ttaggttcta atgatgcaat tagaggttta gaaaaaatat tgtcaaaaca aagtggacat
    16501 agagcaaatg caaataatta tatgggaggt ttgactaatt aatgcaatct tttgtaaaaa
    16561 tcatagatgg ttacaaggaa gaagtaataa cagattttaa tcagcttata tttttagatg
    16621 caagggctga aagtccaaac accaatgata atagtgtaac tattaacgga gtagatggta
    16681 ttttaccggg cgcaattagt tttgcgcctt tttcattagt attaaggttt ggctatgatg
    16741 gtatagatgt tatagattta aatttatttg agcattggtt tagatctgtg tttaatcgca
    16801 gacatcctta ttatgttatt acttctcaaa tgcctggtgt taaatatgca gtgaatacag
    16861 ctaatgttac atctaattta aaagatggtt cttcaactga aattgaagta agtttaaatg
    16921 tttataaagg gtattctgaa tcagttaatt ggaccgatag cgagttctta ttcgactcta
    16981 attggatgtt tgaaaatgga attcctcttg atttcacacc taaatatact catacatcaa
    17041 atcaatttac tatttggaac ggttctactg atacgataaa tccacgattc aagcacgatt
    17101 tgaaaatatt aattaattta aatgcgagtg gaggatttga actggttaac tatacaacag
    17161 gtgatatttt taagtacaac aaaagtatag ataaaaacac tgattttgtt ttagatggtg
    17221 tgtatgcata tcgagatata aatagagtgg gaattgatac aaataggggc attataacat
    17281 tagcgccagg taaaaatgaa tttaagatta aaggagacgt cagtgatatt aaaactacat
    17341 ttaagtttcc ttttatttat aggtaggtga tttaatggat tatcatgatc atttatcagt
    17401 aatggatttt aatgaattga tttgtgaaaa tttactagat gtagattatg gttcttttaa
    17461 agaatattat gaactgaatg aagctaggta catcaccttt acagtttata gaactactca
    17521 taatagtttt gtttttgatt tattgatttg tgaaaacttc ataatttatc atggtgaaaa
    17581 atatacaatt aagcagacag cgccaaaggt tgaaggtgat aaagttttta ttgaagttac
    17641 ggcatatcac ataatgtatg aatttcaaaa tcactcagtg gaatcaaata agcttgatga
    17701 cgacagtagc gaaactggta aaatgccaga atactcttta gatgagtact taagatatgg
    17761 atttgcaaat caaaaaactt cggtcaaaat gacctataaa ataattggag attttaagcg
    17821 aaaagtaccg attgacgaat taggtaacaa aaacggctta gaatactgta aagaagcggt
    17881 agacctgttt ggctgtataa tttacccaaa tgatacagag attggttttt attctcctga
    17941 aacattttat caaagaagcg agaaagtgat tcgatatcaa tataatactg atactgtatc
    18001 tgcaactgtc agtacattgg aattaagaac agctataaaa gtttttggaa aaaagtatac
    18061 agctgaggaa aagaaaaatt ataatcctat tagaacaact gacattaaat attcaaatgg
    18121 ttttataaaa gaaggtactt atcgtaccga aacaattggg tctaaagcta ctattaactt
    18181 tgattgcaag tatggtaatg aaacagttag atttacaata aaaaagggct ctcaaggtgg
    18241 aatatataag ttgattttag acggcaagca aattaagcaa atttcttgtt ttgctaagtc
    18301 ggttcagtct caaacaatag atttaataaa aaatattgat aaaggcaagc acgttttaga
    18361 aatgatattt ttaggagaag accccaaaaa cagaattgat atatcttcaa ataaaaaagc
    18421 taagccttgt atgtacgttg gaactgaaaa atcaacagtc ttaaatttaa ttgctgataa
    18481 ttcaggtcgc aatcaataca aagcaattgt tgactacgtc gcagatagtg caaagcagtt
    18541 tgggattcga tatgctaata cgcaaacaaa tgaagatatc gaaacacagg ataagctgtt
    18601 agaatttgca aaaaagcaaa taaatgatac tcctaagact gaattagatg ttaattatat
    18661 aggttatgaa aaaatagagc caagagatag cgtattcttt gttcatgaat taatgggata
    18721 taacactgaa ttaaaggttg ttaaacttga taggtcacat ccatttgtaa acgcaataga
    18781 tgaagtgtct ttcagcaatg aaataaagga tatggtacaa attcaacaag cgcttaacag
    18841 acgagttatt gcacaagata atagatataa ctatcaagca aatcgtataa atcatttata
    18901 cactagtact ttgaattctc ctttcgagac aatggatata gggagtgtat taatataatg
    18961 gcaacagaag aagttaaaat caaagcgcta cttgaaaacg ataaacagta ctttccagct
    19021 acacattgga aagctataaa tgggatacct tatgcaggca gtagtgatat tgatggattg
    19081 cctcaagacg gtatcatttc ggtagatgat aaaaataaat tagataattt aaaaataggc
    19141 gaagcaggaa ttattcaaaa tagcattgta cagaaatctc caaacggtaa attgtggaaa
    19201 ataacagttg acgatagtgg gaaacttggt acagtgctat tttattagaa aggaaggtgc
    19261 attatggaaa atttgtattt aataaaggat ttgggagctt tagcaggtcg agattataga
    19321 gctaaggaaa tacaaaactt acaaagaata gagcaatttg cgcttggctt gacaacagag
    19381 tttaagttgc atcagaaagc taaaacaatt caacacttcg ctgagcaaat ttattataat
    19441 ggtagatcgc aagcagcagt aaacaaatct ttacaaagtc aaattaacgc acttgttgtg
    19501 gcaccacgta ataacagtgc taatgagatt gttcaagctc gagttaatgt aaacggcgaa
    19561 acctttgaca cattaaaaga acatttagac gattgggaaa ccaaaactca aattaataaa
    19621 gaggaaacta taagagaatt aaataagacc aaacaagaaa ttcttgatat cgagtatcgt
    19681 tttgaacctg ataagcaaga atttttattt gtgacagaac ttgcacctct tacaaatgca
    19741 gtaatgcaat ccttctggtt tgataataga acaggcatag tatacatgac acaagctaga
    19801 aataatggct atatgctaag tcgtttaaga cctaatggtc aatttataga cagctcattg
    19861 attgtaggtg ggggtcatgg tacacataac ggttatagat atattgatga tgagttatgg
    19921 atttatagtt ttatcttaaa tggtaataat gagaatacat tagttcgttt caagtatacg
    19981 cctaatgtgg aaattagcta tggcaagtat ggtatgcaag atgtatttac aggacaccca
    20041 gaaaaaccct acatcacccc tgtcataaat gaaaaagaaa ataaaattct atacagaatt
    20101 gagagaccta gaagtcagtg ggaacttgaa aactcaatga attatataga gataagaagt
    20161 ttagacgatg ttgataaaaa tattgataaa gttttgcata aaatcagtat ccctatgaga
    20221 ctaacaaacg aaacccaacc aatgcagggt gtgacttttg atgaaaaata cttgtattgg
    20281 tatacaggag acagtaatcc aaataataga aactatttaa cggctttcga tttagaaaca
    20341 ggagaagaag cgtatcaggt taatgctgac tatggtggaa cactagattc atttcctggc
    20401 gaatttgcgg aagcagaagg tttgcaaata tactatgaca aagatagtgg taaaaaagct
    20461 ttgatgctag gtgttactgt cggtggtgat ggaaatagaa cacatcgtat tttcatgatt
    20521 gggcaaagag gtattttaga aatacttcac tcaagaggcg ttccttttat catgagtgac
    20581 acaggtggta gagttaaacc tttaccaatg aggcctgata aacttaagaa tcttgggatg
    20641 ttaacagagc caggtcttta ctatttatac actgatcata cagttcaaat tgacgacttc
    20701 ccactaccaa gagaatggcg cgatgcaggt tggttcttag aagtaaaacc accacaaact
    20761 ggcggtgatg tgattcagat attgacgcgt aatagttatg caagaaatat gatgactttt
    20821 gaaagggtgc tttctggaag aactggagac atttcggact ggaattatgt gcctaaaaat
    20881 agtggtaaat gggagagagt accttcattc atcacaaaaa tgtcagatat taacatagta
    20941 ggcatgtcgt tttatttaac tacggatgat acaaaacgtt ttacagattt tacaactgaa
    21001 cgtaaagggg tagctggttg gaacttatat gtagaagctt caaacacagg tggctttgtt
    21061 cataggctag ttcgtaatag tgttacagca tctgctgaga tactattgaa gaactatgat
    21121 agtaaaacaa gttcagggcc atggacttta cacgaaggga gaattataag ttaatgagta
    21181 atttagagaa atctgtagct ataaatttag aaaacacagc gcattatgaa aatatttcaa
    21241 atctagatat aacttttaga acaggagaga gtgaatcttc tgttcttctt tttaatatca
    21301 ctaaaaataa tcaaccgtta ttattgagtg aagaaaatat caaagcacga atagcgattc
    21361 gaggtaaagg agtcatggta gttgctccac tagaaatatt agatccattt aaaggtattt
    21421 taaaatttca attacctaat gatgtaatta aacgagatgg aagttatcaa gctcaagttt
    21481 cggttgcaga attaggtaat tcagacgtgg tagttgtaga gagaactatc acatttaacg
    21541 ttgaaaaaag tttgtttagc aagattccct ctgaaacaaa actacactat attgttgagt
    21601 ttcaagaatt agaaaaaact attatggatc gcgcgaaagc aatggacgag gctataaaaa
    21661 atggtgagga ttatgcgagt ctgattgaaa aagctaaaga aaaaggtcta tcagatattc
    21721 aaatagcaaa atcttcaagt attgatgaat taaagcaact tgctaatagc cgtatatctg
    21781 atttggaaaa taaagcgcaa gcatattcaa gaacattcga tgagcaaaag cgatatatgg
    21841 atgagaaaca tgaagccttc aagcagtcag tgaatagtgg tggtttagtc acaagtggtt
    21901 ctacttcaaa ttggcaaaaa gctaagatta ctaaagatga tggtaagata atgcagatta
    21961 ctggatttga ttttaataat ccagaacaaa gaataggtga ttcaacccaa tttatttatg
    22021 tttcgcaagc tataaattat ccaagagatg ttagtactaa cggtactgtc gaatatttag
    22081 tagtaacttc agattacaag cgtatgactt atcgaccgaa cggtaccaat aaagtgtttg
    22141 ttaaaagaaa agaagcgggt tcatggtctg agtggtcaga attagctatt aatgattaca
    22201 atacaccttt tgaaactgtt caaagtgccc aatcaaaagc taatatggcc gaaagtaacg
    22261 ctaaattata cgcagatgac aagtttaata aaaggtattc ggttattttt gatggaacag
    22321 caaatggtgt gggctctaca ttgtacttaa atgagagttt agaccaattt attttattaa
    22381 ttttttatgg gacttttcca ggtggtgact ttacagagtt tggcagtcct tttggaggag
    22441 gaaagatttc attgaatccc tcaaatcttc cagatggtga tggaaatggt ggaggtgttt
    22501 atgagtttgg attaactaaa tctagtcgta catctttaac tatatcaaac gatgtctatt
    22561 tcgacttagg aagtcgaaga ggttctggtg caaatgcaaa tagagggaca attaacaaaa
    22621 ttataggagt gagaaaataa tgcaaatatt agttaacaag cgtaatgaga taatttcata
    22681 cgctatcatt ggtggctttg aagaaggtat tgatattgaa aatttaccag aaaatttctc
    22741 tcaagttttt agacctaaag cctttaaata ttcaaatggg gaaatagttt ttaacgaaga
    22801 ttattcagaa gaaaaagatg acttgcatca acagattgac agtgaagaac aaaacacagt
    22861 cgcttctgat gacatcttac gaaaaatggt tgctagtatg cagaaacaag ttgttcaaag
    22921 tacaaagtta tcgatgcaag ttaataaaca aaacgctttg atggcaaaac agattgtagc
    22981 attcaataaa aaattagaag aggttaaagg agagacggaa aatgcttaaa ttgatttcac
    23041 caactttcga agatattaaa acatggtatc aattgaaaga atatactaaa gaagatatag
    23101 cgtggtatgt agacatggaa gttatagata aagaggaata cgcaattatt acaggagaaa
    23161 agtatccaga aaatatagag tcataggcca agagtctatg gctttttaat ttgaataaag
    23221 tgggtggcag aatgtttgga tttaccaaac gacatgaaca agattggcgt ttaacgcgtt
    23281 tagaagaaaa tgataagact atgtttgaaa aattcgacag aatagaagat agtctgagag
    23341 cgcaagaaaa aatttatgac aagttagata gaaatttcga agaactaagg cgtgacaaag
    23401 aagaagatga aaaaaataaa gagaaaaatg ctaaaaatat tagagacatc aagatgtgga
    23461 ttctaggatt aatagggacg attctaagta catttgttat agccttgtta aaaactattt
    23521 ttggcattta aaggaggtga ttaccatgct taagggaatt ttaggatata gcttttggtc
    23581 gtgtttctgg tttagtaagt gtaagtaata gttaagagtc agtgctttgg cactggcttt
    23641 ttattttgga taaaaggagc aaacaaatgg atgcaaaagt aataacaaga tacatcgtat
    23701 tgatcttagc attagtaaat caattcttag cgaacaaagg tattagccca attccagtag
    23761 acgatgaaac tatatcatca ataatactta ctgtagtcgc tttatataca acgtataaag
    23821 acaatccaac atctcaagaa ggtaaatggg caaatcaaaa attaaagaaa tataaagctg
    23881 aaaataagta tagaaaagca acaggacaag cacctattaa agaagtaatg acacctacga
    23941 atatgaacga cacaaatgat ttagggtagg tggttgatat atgttaatga caaaaaatca
    24001 agcagaaaaa tggtttgaca attcattagg gaaacaattc aacccagatg gttggtatgg
    24061 atttcagtgt tatgattacg ccaatatgtt ctttatgtta gcgacaggcg aaaggctgca
    24121 aggtttatat gcttataata tcccgtttga taataaagca aagattgaaa aatatggtca
    24181 aataattaaa aactatgaca gctttttacc gcaaaagttg gatattgtcg ttttcccgtc
    24241 aaagtatggt ggcggagctg gacacgttga aattgttgag agcgcaaatt taaacacttt
    24301 cacatcattt ggtcaaaact ggaacggtaa aggttggact aatggcgttg cgcaacctgg
    24361 ttggggtcct gaaactgtga caagacatgt tcattattat gacaatccaa tgtattttat
    24421 taggttaaac ttccctaaca acttaagtgt tggagataaa gctaaaaacg ttattaagca
    24481 agcaactgcc aaaaagcaag cagtaattaa acctaaaaaa attatgcttg tagccggtca
    24541 tggttataac gatcctggag cagtaggaaa cggaacaaac gaacgcgatt ttatccgtaa
    24601 atatataacg ccaaatatcg ctaagtattt aagacatgca ggtcatgaag ttgcattata
    24661 tggtggctca agtcaatcac aagacatgta tcaagatact gcatacggtg ttaatgtagg
    24721 caataaaaaa gattatggct tatattgggt taaatcacag gggtatgaca ttgttctaga
    24781 aatacattta gacgcagcag gagaaagctc aagtggtggg catgttatta tctcaagtca
    24841 attcaatgca gatactattg ataaaagtat acaagatgtt attaaaaata atttaggaca
    24901 aataagaggt gtaacacctc gtaacgattt actaaatgtt aatgtatcag cagaaataaa
    24961 tatcaattat cgcttatctg aattaggttt tattactaat aaaaatgata tggattggat
    25021 taagaaaaat tacgacttgt actctaaact aatagctggt gcgattcatg gtaagccaat
    25081 tggtggagtg gtagctagtg aggtcaaagc gccagttaaa aacgaaaaga atccgccagt
    25141 gccagcaggt tatacactcg ataagaataa tgtcccttat aaaaaagagg ctggtaatta
    25201 cacagttgcc aatgttaaag gtaataacgt aagggacggc tattcaacta attcaagaat
    25261 tacaggtgta ttacctaata acgcaacaat caaatatgac ggcgcatatt gcatcaatgg
    25321 ctatagatgg attacttata ttgctaatag tggacaacgt cgttatatag cgacaggaga
    25381 ggtagacaag gcaggtaata gaataagtag ttttggtaag tttagcacga tttagtattt
    25441 acttagaata aaaattttgc tacattaatt atagggaatc ttacagttat taaataacta
    25501 tttggatgga tgttaatatt cctatacact ttttaacatt actctcaaga tttaaatgtg
    25561 cgtaactggc aggtacttcg gtacttgcct atttttttat gttatagcta gccttcgggc
    25621 tagttttttg ttatgatgtg ttacacatgc atcaactatt tacatctatc cttgttcacc
    25681 caagcatgtc actgggtgtt ttttccttgc gatagagagc atagttttca tactactccc
    25741 cgtagtatat atgactttag cattcccgta taacagttta cggggtgctt tttatgttat
    25801 acttactttt atatagtagg agtgaactat atagccgggc agaggccatg tatctgactg
    25861 ttggtcccac aggagacatc ttccttgtca tcactcgata catatatctt aacaacatag
    25921 aaatgttaca ttcgctacaa ccgtatctta atcgatacgg ttatatttat tcccctacaa
    25981 ccaacaaaac cacagatcct attaatttag gattgtggtt attttttgcg ttttttcggg
    26041 gcgaaaaaag ggcagattat ttgaaaaagg gcaaacgctt gtggaaaatc taaaaggtta
    26101 aaaatgacaa aaaccttgat acaacagtgt ttttggacgc tcgtgtacgt tagagaatga
    26161 ccggtttacc atcttacaag ggtgggatca tgtcgatttt atcggtgtgg atttcctaga
    26221 tttcaagcgt aaaggtgcag aactcgccaa cttctataca ggtattataa atgacttgtt
    26281 gcgtgttgaa gcaactgaag gtaaaggtac acaattgaaa gcaagttaaa ttcattttcg
    26341 taatttaata tgctatgtat ttcgtgctat taacatggca cagcagatat aagtaacatc
    26401 atagtgttga atttcaaaat agttaagtga aataaagcgc ctgtctcatt agcgaaaact
    26461 aaagggacag gcgtatctgt ttatgagctt aataaattgt atgaataata tggttgattg
    26521 aataactgtt tatcatgatg ataaattgag tttttaaaaa taatggtata ttacgccatt
    26581 gttatagcat ttaagaaatc aacaacttta cgataaatag tgattgcttc gtcattaggt
    26641 ctacgatcaa aatcatgctc gtttttattc acgcgttcaa atgttgaatg tggaacatgg
    26701 ttcatgatat gttcgctttc ctcaacggga acatcataat cgccattaca atgcgcaacg
    26761 aacaccgggg gaagtgtttt aagttcattt ggtgagatgt tgtattttga atcagtataa
    26821 tcagcaatgt taatcatatt gatccactca cctgtaccac gtgcataacc gtagattaaa
    26881 aaacgttggg cgatttgatc ttgaataatc ggtgttggtg aagtaagttg agcaagtact
    26941 gtttcattca cactttgagc tattttagcg taatagctat ttgccatttt aaacggttca
    27001 gtattgatgc cactataacc ataaaaatca ataacaccat caatatctct gtctcgtgca
    27061 attaatagac ttaaatatgc acctgatgat ctgccaaagg taaaaatagg gcaattagaa
    27121 tattgtgatt gaatcgcatc gaatgatgcg tagacatcct caataataca atcgagactt
    27181 acttctggta ataaacgata acttagttga attaaatcgt aatgttccgt aaggatatcg
    27241 atatactgtg gcgataaatc attggctttt ccaaacatta aaccgccacc atgaatgtag
    27301 acaatggcac cttttgttgg ttgatgcttc gctttaataa ttgtgtaggg taattcaaat
    27361 gcatctttag taattacttt atctttaatt tcagtcacga ctatacagac tccttatttt
    27421 tgaaattgat gtcattataa cattgtctta aatttctatg aaaaattatc ttttgatgat
    27481 gtgtcatgat aattctgttc taattgacgt aaagcgtcac gggtatgctt ctttagacct
    27541 tccccataat ccatcatttt aacaatatct ttaaaagcgg cacgcggaat ggctaaattt
    27601 tctaaatctt ccatagaaaa ttcaaggtca atatcttgtg gtcgctgttc agcaagttta
    27661 tgcacaaagt caggttctgt gacaaaaggc gaagacatgc cgaccatatc tgcatgttgt
    27721 aaagcatcta aagcagactc tggagaattg atcccgccac ttgcaattaa agggatgcga
    27781 ccagctaaat gttcatagac aatttggtta actggccgac cgaaatgttc gcctggtgta
    27841 cgcgacgtat tttgataaat atgtcgaccc cagctagcga ttgctaaata ttggatgttt
    27901 gaaacgtcca tgacccaatc gattaattga ttgaactcgt caatggtata tcctaaatca
    27961 ctacctctgg tttcttctgg cgttgctcga aatcctaaaa taaaattgtc aggtgcttct
    28021 ttatcaatca cttcttgtac cgcacgcata acttctaaac ataatcttgc acggtttctt
    28081 aatgtgtcgg caccgtaatg gtctgtacgt ttattcgaaa aagttgagaa aaatgtttga
    28141 atcagcaaac gttgtgcaat cgaaatttcc acaccatcaa aacctgcttt aatcgcgcgt
    28201 aatgtagcat cgcgatactg ctgaatgatg ctattgattt tctcatgaga catggcgata
    28261 acatcgtgtt caatcggtga atgcaacgtc atagggcttg gtccatacac ctttccaaaa
    28321 tttaaaattg cttgatttga aaaacgacca gcatgcgcta gctgaataat agcgaggcta
    28381 ccatgttgtt tcatcgtaga cgccatgtta gttaatccag ggatacaagc atcatgatca
    28441 atattaaagc cgtattcaaa caattgacca taaggttcaa tgtaagcagc gccggtgact
    28501 tgcataccag ctgaattaga gcgacgtgca gcataagcca agtctgcttt tgtaatatag
    28561 ccttcttttg ttgatgcgtt tacagtcatt ggtgataata caaagcgatt cgaaattttg
    28621 atgccattag gtaagtggat tgattgtaaa agtggtttgt atcggtacat actatgattc
    28681 cttttctatt caatattgtt ttcaaagtac catggaaaga atgaataatc aatgatgaac
    28741 agtcttgata gaatagaatt ggtacatgga aaacattttt aaaattgaac taatgaatgg
    28801 catttgtagg tctgaaaata tgaatatgaa aaagaaaaat acaagcgaaa agatataaaa
    28861 gttaattgaa aaacgttatc atatacgtgg gtatatgaag agggaatggt attaagaacg
    28921 ctaaaatgtt atgtcggttt gacatgacag gataagtttg gagatgacgg attggttaaa
    28981 ttaagcgtat tagactatgc cttaatagat gaaggtaagg atgcacaaaa ggcattgcag
    29041 gattcagtga cacttgcaaa attagcagat cgacttggct ttaagcgaat ttggtttacg
    29101 gaacatcata atgtaccagc gtttgcgtgt agtagtccag aacttttgat gatgcataca
    29161 ttggcgcaga caaatcacat acgagttggt tctggtggtg tgatgctacc gcactatcga
    29221 ccttataaaa ttgctgagca ttttagaatg atggcagcgt tatatccaaa tcgtattgat
    29281 ttaggtattg gtaataatcc aggtactact atggtaaagc aagctttaga tggaataaat
    29341 cctacatatg atagttacga tgaatcgatt tcgttattac gtgattatct tacaataaag
    29401 gataagccaa gtgcgcatac gttaggtgtc caaccacaca ttgatcattt tccagaaatg
    29461 tggttattaa gtagtagcgc aacatctgct aaaataactg ccgaactagg tatagggctt
    29521 tctgttggaa catttttgct accagatata aatgcgatac atgcagcgaa ggataacatt
    29581 gatatttaca aaaaacattt ccaagcatca acgattaaaa tggacgcaaa ggtgatggca
    29641 tctgtatttg tcattgtagc tgataacgaa gcggaagtag cagcattaca acatgcctta
    29701 gatgtttggt tattaggtaa attacaattt gcagaatttg aacattttcc atcggtagac
    29761 acagcgcaaa agtataagct taatgatcga gacaaagaga tgattcaagc acatcaagca
    29821 cgtatcattg caggtacaca agaacaggtt aaagcgcaat tagatgattt cattgctacg
    29881 tttgaagttg atgaggtgtt agtagcacca cttattccag gcattgaaca gcgttgtaaa
    29941 acattaaaat tactcgcgga aatctatttg tagcatttta aatagaagag aaaggatgaa
    30001 gataagatga aaaagttagc caattattta tgggtagaaa aagtaggaga tttgtatgtg
    30061 tttagtatga cacctgaatt gcaagatgat attgggacag taggttatgt tgaattcgtg
    30121 agtccagatg aagttaaagt ggatgatgaa attgtgagta tcgaagcatc gaaaacggtc
    30181 attgatgtgc aaacgccatt gtcaggaacg attattgagc gaaatacaaa agcggaagaa
    30241 gaaccgacaa ttttaaactc tgaaaaacca gaagaaaatt ggttgttcaa attggatgat
    30301 gtcgataaag aagcattcct ggcattaccg gaggcttaaa tggaaacgtt aaaatcaaat
    30361 aaagcgagac ttgaatattt aatcaatgat atgcgtcgag agagaaatga caatgacgta
    30421 ttggtaatgc catcttcatt tgaagatttg tgggaattat atcgaggttt agcaaatgtc
    30481 agaccggcat tacctgtaag tgatgaatat ttagctgtac aagatgctat gttaagtgat
    30541 ttgaatcgtc aacatgttac ggatttgaag gatttgaagc caataaaagg tgacaatatc
    30601 tttgtttggc aaggtgatat cacgacgtta aaaatcgatg ctattgttaa tgctgcaaat
    30661 agtcgttttc taggatgtat gcaagctaat catgactgca ttgataatat tattcataca
    30721 aaagcgggtg ttcaagttcg acttgattgt gcagaaatca ttcgacaaca agggcgcaat
    30781 gaaggtgtag gtaaagccaa aataacacgt ggatataatt tgccagcaaa gtatataatt
    30841 catacggttg gtccgcaaat acgtcgattg cctgtttcaa agatgaatca ggacttgtta
    30901 gctaaatgct atttgagttg tcttaagctt gctgatcaac atagtttaaa tcatgtcgct
    30961 ttttgctgta tatctacagg tgtatttgct tttcctcaag atgaagcagc agaaattgct
    31021 gttcgaacag tagaaagcta tctcaaagaa acaaattcaa cattgaaagt cgtgttcaat
    31081 gtatttacag ataaggattt acaactgtat aaggaggcat ttaagcgtga tgcagagtag
    31141 taaatggaat gcaatgtctc ttttaatgga tgacaagaca aagcaggctg aagtattgcg
    31201 tactgcgatt gatgaagcag atgcgatagt gattggaatt ggtgcaggca tgtctgcatc
    31261 tgacggattt acatatgtag gagagcgttt tacggaaaat ttcccagatt ttattgaaaa
    31321 atatcgcttc tttgatatgt tgcaagcgag tttacatcct tatggcagtt ggcaagagta
    31381 ttgggcgttt gagagtcgtt ttattacatt aaactattta gatcaacctg taggtcagtc
    31441 ttacctcgct ttaaaatcct taatggaagg taaacattac catatcataa ctacgaatgc
    31501 agacaatgct ttcgatgctg ctgaatatga tatgacgcat gtatttcata tataagggga
    31561 gtatatactg caacagtgta gtcagcattg tcatgctcaa acgtatcgca atgatgattt
    31621 aattagtaaa atggttgttg cgcaacaaga tatgcttata ccttgggaga tgattccaag
    31681 atgtccaaaa tgtgatgccc caatggaagt gaataaacgt aaagcggaag ttggcatggt
    31741 tgaagattca gcatttcatg cgcaactaca acgttataat gcttttctag agcaacatca
    31801 agatgataaa gtgttgtatt tggaaattgg aattggttat actacaccac aatttgtgaa
    31861 gcatcctttt cagcgtatga cacgtaaaaa tgaaaatgca ctttatatga caatgaataa
    31921 aaaggcatat cgcattccga attcaattca agaacgtacc atacatttaa ctgaggatat
    31981 ctcaacattg attacagcag cactccggaa cgacagcaca acgaaaaata acaacattgg
    32041 agagacagaa gatgtactta atagaaccga ttagaaatgg agaatatatt actgatggtg
    32101 cgattgcact cgctatgcaa gtttatgtta accagcatat ctttttagat gaagatattt
    32161 tattccctta ttattgtgat ccaaaagtgg aaattggacg ttttcaaaat actgctatag
    32221 aagtgaatca agattatata gataaacaca gtattcaagt agttcgccga gatactggtg
    32281 gtggcgctgt gtatgttgat aaaggtgccg ttaatatgtg ttgtatttta gagcaagaca
    32341 cttcaattta tggtgatttt caacgatttt atcagccagc tataaaggcg ttgcatacat
    32401 taggtgcaac agatgtgata caaagcggta gaaatgattt aacattgaat ggtaaaaaag
    32461 tgtcaggcgc cgcaatgaca ttattgaaca atcgtattta tggcggttat tcgctattac
    32521 ttgatgttaa ttatgaagca atggatgaag tgttaaagcc taatcgcaaa aagattgcat
    32581 cgaaagggat taaatctgtg cgcgcacgtg ttggtcatct tagagaagca ctggatgaaa
    32641 agtatcgtga tataaccatt gaagaattta aagatttaat ggtgacgcag attttgggaa
    32701 tcgatgacat taaagaggca aaacgatacg aattatctga tgcagattgg gaagcgattc
    32761 aagaattggc gcgcaaaaag tataaaaatt gggattggaa ttatgggaag tcacctaaat
    32821 atgaatataa tcgaagtgaa agattatcat caggtactgt agatattacc atttctgttg
    32881 aacaaaatcg tattgcagat tgtagaattt ttggtgattt ctttggacaa ggtgatataa
    32941 aagatgtgga acaagcatta caaggaacaa aaatgacaag agaagattta acgcatcagt
    33001 taaagcaatt agacatcgtt tattattttg gtaatgttac ggtagaatca ttggttgaga
    33061 tgattttaag ttaatattgg tattttatgt atgctgaatc attggaagtg tttgcttgct
    33121 cttgaaaagg tgacaatagt gtttgatgaa ggttgaacat atgagtggaa attattgcct
    33181 ttaactattc aaagtatgat atatatggtt tttgtttcta aatgattggg tatttgaaaa
    33241 tagatgagtt taatatttta aggaatataa tgatgtttgc ttttataatt catatagaat
    33301 attaagcaat ataagtctgt tgatatatac aaaaatataa tgactgctat aatgaataat
    33361 caatagacac aaagaggaga ttatgtgatg aataataaag tattagtgac cggtggtaca
    33421 gggtttgttg gtatgcgaat aatttcacga ttattagaac aaggttatga ggtacaaacg
    33481 acgatacgtg atttaagtaa agctgataaa gtaattaaaa cgatgcaaga caatggcatt
    33541 cccacagagc gattaatgtt tgtcgaagca gatttatcac aagatgaaca ttgggatgaa
    33601 gcaatgaaag attgtaaata tgtgttgagt gtggcatctc cggtgttttt cggtaaaaca
    33661 gacgatgcag aagtgatggc gaagcctgcc attgaaggca tacaacgtat tttaagagct
    33721 gcagaacatg ctggcgtgaa gcgtgtggtg atgactgcaa actttggtgc agttggtttt
    33781 agtaataaag ataaaaattc aatcacaaat gaaagtcatt ggacaaatga agatgaacca
    33841 ggcttatcag tatatgaaaa atcaaaattg ttagctgaaa aagcagcgtg ggattttgtt
    33901 gagaatgaaa atacgacagt agaatttgcc acaatcaatc cagttgcaat ttttggacca
    33961 tcattagatg cacatgtttc aggaagcttt catttattag aaaatttatt gaatggttca
    34021 atgaaacgtg tacctcaaat tccattaaat gttgttgatg tgagagatgt agcagaacta
    34081 cacattttgg caatgaaaaa tgaacaggct aatggcaagc gatttattgc gacggctgat
    34141 ggacaaatta atttgttgga aattgcaaaa ttaattaaag aaaagagacc tgaaatagct
    34201 caaaaagttt ctactaaaaa attatcagac tttattttga gtctaggtgc taaatttaat
    34261 catcaagcta aagaaggtaa acttttatta gatatgaatc gaaatgtaag taacgaacgt
    34321 gcaaaaacac ttcttggttg ggaaccgatt gcgacacaag aagaagcaat tttagcagca
    34381 attgatagta tggctaaatt tcatttaata taatacaaaa acaccgtcta taagtaagtg
    34441 gctttcctct tgataaaagg ggcattcact caatatgtga cggtgtttta tatttttacg
    34501 ttaacactat tactgtggct tttgacgacc ttttaataaa attgctgtcg cacctacgat
    34561 aataataaat agaatcgcac caaataatcc catatatttt actgcgttac cgaacacgat
    34621 accgacagct aaaaagtctg tatctgagaa tgttgttgca gcaccaccta attcgcctaa
    34681 aaatggcaag aataataatg gtaaaaacgt gattaggata ccatttagag cggcgccagc
    34741 aacagcacct ttaataccgc ctcttgcatt accgaataca gcagccgttg cacctaagaa
    34801 gaagtgtgca actacgccag gtaaaatgac gacgccacca aataagaata agataaacat
    34861 accgatgaca cctgtaataa agctgacaaa gaatccaatt agtactgcat tttgtgcata
    34921 agggaacaca atagggcagt ctaatgcagg tttagaattt ggtacaagct tttcagaaat
    34981 ccctttaaat gctgggacga tttcagctaa gattaagcga acgcccgtta aaataataaa
    35041 tacaccagca gcaaatgtca caccttgaat taatgaaaag acaataaagt tttgaccatc
    35101 actaatagat tcgtgtacat aactaacacc cgcaaataag catgcgatga agtaaagtaa
    35161 tgccatcgta atcgagatac taattgtact ttctcgtaag aaacttaagc cttttggaaa
    35221 tttaatctct tccgttgatt tagacttccc tttgaataat tgacctacag cacctgcggc
    35281 aaagtaactg attgagccaa aatgacctaa cgctacttga tcattccctg taatttttcg
    35341 catcgtaggt tggagtaatg caggtaagac tgccatgatt aatcctaata cgagtgcgcc
    35401 gataacaatc gttagccagc ctttaatatg actgactgtt aaaatgattg ctaaaaacgc
    35461 agccatgtaa aatgtatgat gacctgttaa aaagatatat tttaaattag tgaagcgggc
    35521 aattaaaata ttaacaatca tgccacagac catgatgagt gcagctgttg ttccaaaatc
    35581 ttttaaggct agtgagacga tagcttcgtt gttaggtacg ataccttgca caccgaatgc
    35641 gtgttggaat attttgccga atggctcaag agatcgaacg acgacatcag cacctgcact
    35701 taaaattaag aagcctaata tcgttttaat ggttcctgaa gtgattgttg cggcaggttt
    35761 tttctgaacg attaaaccta taaaggcaat cagtgcaaca agaatggctg gttgacttaa
    35821 aatatcgact ataaaattaa ggattgcttg cataggtcgt acctccttta aatcatgtta
    35881 agttgttgta atttttctgc gagcttttgt tgtaattcag ctttgtctaa aatattatca
    35941 agaactaaga catcccctag acgttcggca ttttcagcta aatctctacc acaaataaac
    36001 aagtcagcca tctctggact tgctgtcata atgtcactat gttcaacttc gatatcagat
    36061 ggtgcattaa gttgcctaag tgcttcttgt gcgttcattt ctaccataaa actacttcct
    36121 aaaccgtggc cacatactac taaaattttc atattaatca tgctccttta aaatgttttt
    36181 aatgtcttgt gcgtttgttg cagttaatag ttgctggact gtttggttat cgcccagtac
    36241 ggttgctaaa ttttgtaata cagataagtg tgaatgattg tcgatggcac tcaatacaaa
    36301 aatgagagat gcgtagtgat cttcatcaca aaatgccaca tgttgattca actttaatag
    36361 acttaaacca acttgatgta cgtcattgtt cggtcttgca tgtgcaattg caatttcagg
    36421 tgcgataacg atataaggtc caagttcatt aacgctatca atcattgctt gaacatagcc
    36481 ttgttcaata atttgttctt gtagtaatgg ctgagaagct atagttatag cttcagtcca
    36541 atcatttact tgttctttta caatgatgcg tgttgttgac aaaatgtcta atgacacgtt
    36601 attaaacctc ctttgtcata gttaaagcaa tgtgttgttt aattttaaaa atattcccat
    36661 ctaagaaatc ttgtcgatat aagtcgttgc ttaagcattc gcttaactgt cccaatgcct
    36721 ttaaatgtgc attggggtgg tccgttgcta atgtaattac aaggtgaacg ggatcgttag
    36781 ctttactacc aaaaataatc ccttcagtga aataagttag tgcgaaacct acaccatttt
    36841 gtacataatc agtaccagcg tgaataagtg caatatgtgg actaatgacc atatatgacc
    36901 cgaattgttc aaattgtttt aaaattgcag ctgtataatt tgaattgaca atgccatcat
    36961 tgattaaagg ttgcacagcc actgtaattg ccgattcaat tgataatggt tgtttattta
    37021 taatgatgcg atgttcaggt aataaatctg cgagtgactt gccatcagtt gccattttca
    37081 tgactcgttg ttctcttgcg tcattgataa tttgattcaa tttttgacga gattgctgat
    37141 tgataaatgg atcgacatga ataactggta cagctgatat ttcacaaggt actgttgaaa
    37201 tgacataatc aatgttatct tgcaataatc gactttcttc caattgataa atggaatagg
    37261 catcccaaat gtgaaactca ggatacaggt gatttagttt tgattttaaa agttgcgacg
    37321 tgcctatacc agaaccacat agtaagacaa ccttaatcat tgattgttta tgtgttgcaa
    37381 cacgctctat acttgatgcg aaatgaattg taatgtatgt taattcatct tcgttgaagc
    37441 gaatagcagc atcttgttca attgggctaa tatgcttgct aacggcttca atgatttgag
    37501 gatagcgacg cataacttct tgcctcaaag gattaggttg tagcatatcg tatttaatac
    37561 gatgtatagc tggtttgata tgtgtgatca gactggtatg caacttgttg tcttttgaca
    37621 tatcaatgcc taattcttgg ctaacacaag tgatcaattc atgtatattt tgcgataaat
    37681 catggtattc aaaggtaatt gaagatgctg tatgttcggt cattttagag cctagtaaat
    37741 gtaacgtgat aaagataatt tcagactctg gaaatgtgac attacaactg cgttctaagt
    37801 tttctatcat ttttgaagca atagcatact gattagtatg tcgccattta tcaatttcat
    37861 tgataggtat atcgaacgaa aaattttcat ttaaacgctg aatggcaatg agtatatgat
    37921 agattaagcc atcgatagcc gactgaacta aatgataatt ttcactattt aatgttttaa
    37981 taatggcacg gcgaaccaat gcgatagatt ctgaattaaa gatatcagcc tctataaaag
    38041 gtgcagcttg tttcatatat tgatgtataa agtgtgcata cgctttacga taatgatctt
    38101 cctcaccaat aatattgaat cctttattgt ggacataatt taactttaaa tggtattgat
    38161 ctagttgggc ttgaatcatt ttaatatcat ctgcaattgt ccgacgcgaa acattaacat
    38221 cttgtgcaag ttgctttgtt gaaacaggat cagttgtttc gaataacttt aaagcgatat
    38281 gtgtgagtcg ctcatctttt gaaaaatgaa tttgattcgt tagattgtgc tctaattcat
    38341 ttaatatcgt tgtgttagct gttgttactt tgatgcctgc agctttatta cggctgactt
    38401 ggtaatgata agtttcagca tattgctcaa tatatgctat atcatattga atggtacgag
    38461 gtgatacacc aagttgatta gcaatggtat tgattggaat aaacgtttgc tcatgaatta
    38521 aaagatacaa aatttcgatt tgtctataac ttaacaacgt aatatcctcc tatttgtaat
    38581 tgtaagcgat ttctcgaaaa cgtagatatg caatctcttt catattttaa tccgaaaaat
    38641 tgcatatcaa aatgtttatg gcgcaagatt ttataggaac ttttaaaata aattagatat
    38701 tcatgttgac aatttaaaaa tgtcgcagta tatttagtta gacatctaac gaaatggtgg
    38761 tgcaataaat ggaattcact tattcgtatt tatttagaat gattagtcat gagatgaaac
    38821 aaaaggctga tcaaaagtta gagcaatttg atattacaaa tgagcaaggt catacgttag
    38881 gttatcttta tgcacatcaa caagatgggc tgacacaaaa tgatattgct aaagcattac
    38941 aacgaacagg tccaactgtc agtaatttat taaggaacct tgaacgtaaa aagctgatct
    39001 atcgctatgt cgatgcacag gatacgagaa gaaagaatat aggactgact acctctggga
    39061 ttaaacttgt agaagcattc acttcgatat ttgatgaaat ggagcaaaca ctcgtatcgc
    39121 agttatctga agaagaaaat gaacaaatga aagcaaactt aatgaaaatg ttatctagtt
    39181 tacaataaat ggtaagcgtg actggtagaa atcagtcact ttatctttaa tgttatagtt
    39241 agatatctaa ttgttagtaa gctaattatt aaaaaagaca aggagtattg aacaatgaaa
    39301 gacgaacaat tatattattt tgagaaatcg ccagtattta aagcgatgat gcatttctca
    39361 ttgccaatga tgatagggac tttattaagc gttatttatg gcatattaaa tatttacttt
    39421 ataggatttt tagaagatag ccacatgatt tctgctatct cactaacact gccagtattt
    39481 gctatcttaa tggggttggg taatttattt ggcgttggtg caggaactta tatttcacgt
    39541 ttattaggtg cgaaagacta tagtaagagt aaatttgtaa gtagtttctc tatttatggt
    39601 ggtattgcac taggacttat cgtaatttta gttgctttac cattcagtga tcaaatcgca
    39661 gcaattttag gagcgagagg tgaaacgtta gctttaacaa gtaattattt gaaagtaatg
    39721 tttttaagtg caccttttgt aattttgttc ttcatattag aacaatttgc acgtgcaatt
    39781 ggggcaccaa tgatttctat gattggtatg ttagctagtg taggcttaaa tattatttta
    39841 gatccaattt taatttttgg ttttgattta aacgttgttg gtgcagcttt gggtactgca
    39901 atcagtaatg ttgctgctgc tttgttcttt atcgtttatt ttatgaaaaa tagtgacgtt
    39961 gtgtcagtta atattaaact tgcgaaacct aataaagaaa tgctttctga aatctttaaa
    40021 atcggtattc ctgcattttt aatgagtatc ttaatgggat tcacaggatt agttttaaat
    40081 ttatttttag cacattatgg aaacttcgcg attgcaagtt atggtatctc atttagactt
    40141 gtgcaatttc cagaacttat tatcatggga ttatgtgaag gtgttgtacc actaattgca
    40201 tataacttta tgtcaaataa aggtcgtatg aaagacgtta tcaaagcagt tatcatgtct
    40261 atcggcgtta tctttgttgt atgtatgatt gctgtattta caattggaca tcatatggtc
    40321 ggactattta ctactgatca agccattgtt gagatggcga catttatttt gaaagtaaca
    40381 atgacatcat tattattaaa tggtataggt ttcttgttta ctggtatgct tcaagcaact
    40441 ggtcaaggtc gcggtgctac aattatggcc attttacaag gtgtggttat cattccagta
    40501 ctatttatta tgaatgcttt gtttggacta acaggtgtca tttggtcatt attaattgct
    40561 gagtcacttt gtgctttagc agcaatgtta atcgtctatt tattacgtga tcgtttgaca
    40621 gttgatacat ctgaattaat agaaggttaa atatttcgta cacttctgac tgagtatatt
    40681 tcggtcggaa gtgtattttt cgaaaaaata aatatatgat acgattatga aaaaataaag
    40741 tgagattggc atatgtataa atctaaaata ctgttgaaat atatttttag tgaggaatca
    40801 gaagttaaag atttaactga agaaaaatat aatcaagatt acgaagcatt aacatttagc
    40861 tttaaagagg aaacatatca aagtaggtta gctaagaaaa caccgactaa agcagggtat
    40921 tttgttacat gttggacaaa agacgagaac aattgtaatc aaccatactc aaaagaggca
    40981 tttgcagatt acttaatgat tatagttatt gatgaagaat taagcggtta tttcttattt
    41041 cctagggaat tattggtaga aaaaggcatc ttaactacat ttgagcataa aggtaaaatg
    41101 gcttttagag tgtatcctaa gtggtgtaat caattgaata aaacagcagg gcaaacacaa
    41161 aagtggcaat gtaaatattt ttttgaatac taataaaaag tcatatggtt gaacttataa
    41221 gagatgaata tatcatcttt cgataagata accatatgac tttatttttt atcatttaat
    41281 gatgaacggt ttcttgtcct actttattcc aagtgaggat aaagctcaac attgcaaaca
    41341 cactgattgc tgttaataaa ataaaaccga catcccatcc gaatttatca actacagcac
    41401 ctaagacgat gttggccatt acagcaccaa acagataacc aaataatcct gttaatccag
    41461 ctgctgtgcc agctgctttt ttaggaacat aatctaatgc ttgtaaacca attaacataa
    41521 ctggtccata tattaagaaa ccaatggcaa ttaatgagac attgtctaac caagcattgc
    41581 ctggaggatt taaccaataa attaatacaa atactgtgac acctaacata aagaagaaac
    41641 ctgcaggtcc acggcgacct ttgaataatt tatcagaaat gtaaccacat aataatgtac
    41701 caggaattcc agcccattcg tataagaagt atgcccaacc tgatgctttt aagtcgaaat
    41761 gtttttcttc acttaagtag actggcgccc aatcaagtac accataacgc acgaaataaa
    41821 caaatatatt tgcgaaggca attgcccata cccatttatt gttcagtaca tatttaaata
    41881 aaatttcttt tgtagttaat tctgtttcta atgttttctt atcgcttgta gcaaagtcat
    41941 ttttataaat ttcgattgga ggtaaacctt gagattgagg tgtgtctcta atcaatacgt
    42001 atgaaattgc ggcaatgata agtgctaaga gtgcagggta aatgaataca ccttcgaaac
    42061 cttttaaata accaaagttg ataaatgctg ttgttgtaat accccaagca gcaataggag
    42121 ccataatacc tccaccaaca ttatgcgcaa cgttccaaag ggcagtctta cttccgcgtt
    42181 cacttacact aaaccagtga acgagaacac ggcctgaagg tggccagccc ataccttgaa
    42241 accatccatt taagaataat aggacaaaca taataccgat acctgatgta aagaacggta
    42301 caaatcccat taacaaattg acgatagcag tgagtactaa tccaagaact aagaatatcc
    42361 gagcattgct ccgatcactt acagtaccca taaagaactt actaaatcca tatgcgatgg
    42421 aaacagcaga aagtgcaaaa cctagttccg cttttgtaaa accttgctct tgcaatgccg
    42481 gcatcgctaa tgaaaagttt ttacgtaata aatagtaccc agcgtaaccg atgaaaatac
    42541 caagaaatac ttggagacgt aatcgtttat aggtatcatc tatctgattt tctggcaaag
    42601 gcttaatatg ctttgcaggt ttaagaatat tcataaaatc ctccttaata tgtatttata
    42661 tgcattttgt gcgataaact tatattagac atgtatgaaa gcgttgtcaa tatacttttg
    42721 tgaaatcttg tagatttagt tattgaagta ttttataaaa atttagggaa aataaatttc
    42781 cacacataaa tttataatta tgcaattatt atgtcgttat acatactagc tgtgtatcta
    42841 cgaatgagtg caagcatatt tttatttgca gaaaaggggt aaatggcata tagctatctt
    42901 ttttatgtaa gttggtataa aatttatact aataggaggg atagtatgaa tatagtaggg
    42961 catcatcaca tatccatgta tacaaaagat gcaaaacgta ataaggattt ttacacaaat
    43021 gtccttggat tacgattggt tgaaaagtcg gttaatcaag acaatccttc aatgtatcat
    43081 ttgttttatg gggacgaagt aggtacagcc ggaacaattt taagcttttt tgaaattccc
    43141 aatgcgggtc ataagcagcc aggtactgaa acgatttatc gattttcatt attagtacca
    43201 aatcaagcgg cacttcatta ttttgaaaaa cgtcttgaga ataatggtat taagtctgaa
    43261 cgtttgtact atcttagacg agaaggtgtt gtctttaaag atgaagacga cttagaaatc
    43321 atactgcttg ttaatgatag ttttgaagta ccacatcaat ggcaacataa cgcttatagt
    43381 gaaatacctc aagcatatca aattttagga atagggccag tagaattaag agttagaaat
    43441 gcagcgcgta cggtagaatt tttggaaaat gtcttaggtt atcgtaaaag agataataaa
    43501 tcattcgatg tgctgacatt agcaccacaa ggtttatatt cggattttgt agttattgag
    43561 caacagggac aacgtgaaag acctggacga ggttatatcc atcatattgc agttaataca
    43621 ccaaaaatga gtgacttaga tgcaatttac aagaaattac aacaacaacc acaaagtaat
    43681 tcaggtataa ttgatcgcta tttctttaaa tcattatact atcgccataa ttcaattatg
    43741 tatgaatttg cgactgaagc gcctggattt actattgata cacctgttga acaattagga
    43801 agtcaattga acttgcctga ctttttagaa gcagaacgtg aacaaattga aagtaagtta
    43861 cacgaaatat aaaggagaat gtttaatggc aaaattagaa atggataaaa atacgcctct
    43921 tgagtttggt ttgtattcct taggtgatca tttattgaat ccatttaaag gtgaaaaagt
    43981 tagttatgag caacgtatta atgaaattat tgaagcaagt aaattagcag atgaagcagg
    44041 tattgatgtt tttgcagttg gtgaaagtca tcaggagcat tttacaacac aggcacatac
    44101 ggttgtgtta ggtgcaattg cccaagcgac aaagcatatt aaagtttcaa gttcttcaac
    44161 gattattagt gcaacagatc ctgtaagagt atttgaagac ttcgcgacat tagatttgat
    44221 ttctcatggt agagccgaaa ttgtggctgg tagagcatca agaacaggta tttttgactt
    44281 gtttggctat gatttaaaag actatgatga attgtttgaa gaaaaattag gtttactttt
    44341 agagttaaat aaaactgagc gtattacttg gtctggaaaa tatcgtccag aacttagaaa
    44401 tatgaaaata ttcccaagac caatcgataa tacattgcca atatggcgtg ctgttggtgg
    44461 tccacctgca agtgctatca aagcgggaaa acaaggtgtg ccaatgatga ttacaaccct
    44521 tggtggccca gcaatgaact ttaaaggttc tatagatgct tatcgtcaag tagcaactga
    44581 agcaggtttc gatgcttcgc ccaagtcttt accagtaagt acagcgagtc tgttttatac
    44641 agctgaaaca actcaggatg ctatgagaga attttatcca catttgaata cagggatgtc
    44701 atttattcgt ggcgttggtt atccgaaaca gcaatttgct aattcaccag attatagaga
    44761 agcgctaatg gttggaagcc cgcaacaaat tattgaaaag attttgtatc aacacgagtt
    44821 gtatggtcat caacgtttta tggcacagct tgattttggc ggtgtgccat ttgaaaatgt
    44881 tatgaagaat attgagttaa ttggtaacga cattataccg gcgattaaaa agcatttatc
    44941 aaaataggag gggcgtcatc atgaatattg tattattgtc aggttccaca gtaggttcta
    45001 aaacgagaat tgctatggat gatttaaaaa atgaactaga agtcatcaat gagggacatc
    45061 aaatagagtt gatggattta cgagaacttg aattagaatt tagcgttgga aagaattatc
    45121 tagatactac aggagatgta tataaattaa cgacgtcgtt aatgcaggct gatgtgattt
    45181 ttattggttt tccaattttt caagcttcca tccctggtgc tttgaaaaat gtatttgatt
    45241 tacttccagt caatgcgttt cgtgacaagg taataggact tgtagcgaca gcaggttcta
    45301 gtaaacatta tttaattcct gaaatgcatt taaaaccaat attgagttac atgaaagcac
    45361 atacgatgca aacgtatgta ttcattgaag agaaagactt ttcaaatcaa caaattgtca
    45421 atgatgatgt tgtatttcgt ttaaaagcgt tggcacaatc cacaatgcga actgccaaag
    45481 tacaacaaca agtgcttgaa gaagaaaata accaatacga cttttaaagt ataaaaataa
    45541 gacgctcggc acactaaatt tgtaagtgtt tgagcgtctt ttcatattaa ctatatagcc
    45601 agtgaacgac gataaaggca agtgatgaca agcatattga ggtaataatg attgtcataa
    45661 gcggtttaag tgcgcgattt ttaagatctt taaatgcaac atttaaccct aaagcaacca
    45721 tggccattaa taagcaaatg gttgatacag tatttaaaat atttagcaat gctgacggga
    45781 tagttacata tgtattcact aaggccataa tgacaaatcc aattaaaaag tatggaatgc
    45841 ttattcgacc cttgctagat gattctgatg aacggaagcg cataattaaa ataagtacga
    45901 tggttaatgg aatcagtaag aatactctac caagtttacc aagaagtgcg attttaagtg
    45961 catcactacc accaaagcca ccagctaaga caacgtgtgc aatttcatga agactaacgc
    46021 cagaccaagc gccataaaca tttgtcgtca ttgaaaagat agcgtagata gctgtatata
    46081 taagtgaaaa tatcgtacca atcaatgcga tgataccgat actaatagct gtatcttttt
    46141 cacgtgattt gaatattgga gctactgcag caatagcggc agcaccacaa acgcctgtac
    46201 ctacacctag taataatgcg atgtttttgt caccatgcaa cagtttgttg acaaagagca
    46261 tcattacaat actgaaaata acgacaccta catcgatggc taatagtcta ctaccttgac
    46321 cgataatatc gaatatattg agtttaagtc catataggat gattgcaaat cttaataaat
    46381 atttagatga aaacgtaata cctgagctat attgttcagg atatcctcta aagtgacgat
    46441 atagaatagc gattaatatc gcgatagtta atgcgccaac cttatctaag attggtaatt
    46501 tagctgctaa aaagctaaat aatgcgacta taaatgttaa tgaaagtcca atcataaaat
    46561 gcttattttt caatgatgcc atgagcagtg cctcctttaa tagcatttta gcactgtttt
    46621 gtcgtatttt taaatataaa tttggaatga ataataaagt agtgattaaa ttaagttgtg
    46681 tgataggtat caagggcatc aaccaaagta ataatcacta caacgcttat tggcggggcc
    46741 ccaacaaaga agctgacgaa aagtcagctt acaataatgt gcaagttggg gtgcggggcc
    46801 caacaaagag aaattggatc cgtaatttct acagacaatg caagttggcg gggccccaac
    46861 atagagaatt tcgaaaagaa attctacagg caatgcaagt tggggaagaa caacaaattt
    46921 aagatacaag gcgtaatatt aatatgttat tattatgata atttacagaa ttatatgaaa
    46981 aatgaatgag ggtgtgatgg tatgtttgga atgaaagtga atgaacaaat aacattaaaa
    47041 attttagaag tccatgacac agaagcactt ttcaatttag tcaatcgttc aagaaattca
    47101 cttagggaat ggttaccttg ggtagatgca actgagcaat catcagatac gcgtgcgttt
    47161 attaaaagag ggcttttgca atttgctgat ggtaatggat ttcagtgtgg catttggtat
    47221 ggaggaacgc tagttggtgt catcggtttg catgaaatta atcacatgca cagaaaaact
    47281 tcattagggt attatttaga taaacaatat gagggtcatg ggattatgac acaagcagtt
    47341 gaggcattga taaagtattg cttcgatgag attgacttaa accgaattga gattagtgtc
    47401 gcagttaata atgaaaaaag ccaagctatt cctgaaaggc tgggatttac tagagaaggt
    47461 atgttacgtg acaatgaatt actaaatggt atttattcat cgagttacat ctatagttta
    47521 ttaaaatcag aatttaatga aggatgataa aacagactac aaaagagtaa tgacttacat
    47581 ataagtgtta ctcttttatt tatttttaaa gaaaaaaggt tgttgacagt attattttat
    47641 aacaatataa tgattttgat aattattatc aactagataa tgttcatggg aggatgcctt
    47701 aaaatcatca ttttaagagc cacgtattat cttggcattt agggagtgtg agatagtacg
    47761 aatgagggca tgtctatggg gataatagaa tttctataat gaggtgtcaa aatgaaaaag
    47821 ttaacaacgc tattattagc atcaacgtta ttaattgctg catgtgggaa cgacgatagt
    47881 aagaaggatg attcaaaggc atcgaaaaaa gatgatggtg ttaaagcaga attaaaacaa
    47941 gcaacaaaag catatgataa atatactgat gaacagttaa atgaattttt aaaaggtaca
    48001 gaaaaatttg ttaacgcgat tgaaaataat gatatggccc aagcaaaagc gttatatcca
    48061 aaggttcgta tgtattatga acgctctgag ccggttgcag aagcatttgg agatttagat
    48121 cctaaaattg atgcacgtct tgcagatatg aaagaagaga aaaaggaaaa agaatggtca
    48181 ggatatcata agattgaaaa agcattatac gaagataaga aaattgatga tgtgactaaa
    48241 aaagatgcac aacaattatt gaaagatgca aaagaattgc atgccaaagc tgatacatta
    48301 gatatcacac caaaattaat gttacaaggt tctgttgacc tattaaatga agttgcaact
    48361 tctaaaatca caggtgaaga agaaatttat tcacatacag atttatatga ttttaaagcg
    48421 aacgttgaag gcgcacaaaa aatttatgac ttatttaaac ctattttaga gaaaaaagat
    48481 aaaaagttaa gtgatgatat tcaaatgaac ttcgataaag tgaataaatt attggataaa
    48541 tataaagata acaacggcgg ttatgagtca tttgaaaaag tatcgaagaa agaccgtaaa
    48601 gcatttgcgg atgctgttaa tgcattagga gagccactaa gtaaaatggc tgtgattact
    48661 gaatgacaaa ttatgaacaa gttaacgata gtacgcaatt ttcaagacgt acatttttga
    48721 aaatgttagg tattggcggt gccggtgttg caattggcgc aagtggtgtt ggaagcatgt
    48781 ggtctttcaa atcaatgttc aatacaccag aagatccgga aaaagatgcg tatgaattct
    48841 atggtaaagt acaaccaggc attaccacac cgacgcaaaa aacatgtaaa ttcgttgcat
    48901 tagatttgaa atcaaaagat cgagatgcga ttaaagcaat gtttaagaaa tggacggtta
    48961 tggctgatcg tatgatggat ggagatacag ttggcaaggc cagtaacaat cctttaatgc
    49021 caccagtaga tactggtgaa tcgattggat taggtgcaag caagttaacg attacctttg
    49081 gaattagcaa gtctttgatg aagaaaattg ggttatctag taaaattccc gatgccttta
    49141 aagatttacc gcattttccg aatgatcagt tactagacga ttacagcgat ggcgatatta
    49201 tgattcaagc atgctcaaat gattcgcaag tgtcttttca tgcggttcat aatttagttc
    49261 gtccatttcg agatattgtt aaggtgcgtt gggcgcaatc tggttttatc tctgctaaag
    49321 gtaaggaaac gccaaggaat ttaatggcat ttaaagatgg aacaattaat cctaggaaga
    49381 ataatcaact taaagattat gtgtttattg atgatggatg ggcgaagcat ggaacttatt
    49441 gtgttgtcag acgtatccaa atacacattg aaacgtggga tcgtactgca ctggaagaac
    49501 aagaggcaac atttggtcgg aaacgacata gtggtgcgcc gttaacaggc gggaaagaat
    49561 ttgatgaaat tgacttaaaa gcgaaagata gtcatggcga atatattatt gataaagatg
    49621 cccatacgag actagcgaaa gaagctaata cgtcaatttt acgtagagcc tttaattatg
    49681 tggatggtac tgatgatcgt acaggtaact tcgaaacagg cttgttgttc attgcttttc
    49741 aaaaagcgac ccaacaattt atcgatattc aaaataattt aggcagtaat gataaattaa
    49801 acgaatacat tacacataga ggttctgctt catttttagt attaccaggt gttagtaagg
    49861 gaggatacct cggtgaaaca ttatttgact aaaattatag taatgctaat tactgctgct
    49921 atggtgtgta actttgggtt attgaaaagt caggcagcag aacaacaaag tattagtgat
    49981 gtatatagtg tgataacgga tgcgaaatct gcactttcta ataattcgat atcgaatgac
    50041 aataagcaga aagcaattga gcaagtggta agtgcagtta agaaattatc gcttgaagat
    50101 aatagtgaag gtaatgctgt caaatcagat gtgagaaagc ttgaagatgc aaaagcgaat
    50161 gatgatcaaa aagatacact ttcgcaatta acgaagtcat taattgctta tgaagagaaa
    50221 ttggctagta aagatgcggg ttctaaaatt aaactattgc aacagcaagt cgatgccaaa
    50281 gatgcagcga tgacaaaagc gattaaagac aaaaataaag cggaattaga atctttgaac
    50341 aatagtttga atcagatttg gactagtaat gaaacagtga ttcgcaatta tgacgcaaat
    50401 caatatggac aaattgaagt cgcattatta caacttagaa ttgcaattca taagtcacca
    50461 ttagatacga caaaagtgtc acatgcttgg acaactttca aatcaaatat tgatcatgtc
    50521 gataagaaaa gtggtacgtc tgcaaatgat caataccatg tatcacaatt aaatgatgag
    50581 ttagagaagg cgattaaagc tatcgacaac aatcaattgt cggatgctga tactgcgctt
    50641 acacatttta tagaaatttg gccatatgtt gaaggtcaaa ttcaaactaa agatggtgct
    50701 ttgtatacga aaattgaaga taaaatacca tattatcaaa gtgtattaga cgaacataat
    50761 aaagcacatg tgaaagatgg tttagtaggt ttaaataatc aaattaaaga ggttgttggc
    50821 catagttata gcttcgtcga tgtgatgatt atctttttac gtgaaggtct agaagtgtta
    50881 ttaattgtaa tgacattgac tactatgacg cgtaatgtca aagataataa gggtactgca
    50941 agtgtgattg gtggtgcaat tgccggactt gtattgagta tcattttagc aattacattc
    51001 gtagaaacac taggaaatag cggtatgctt cgtgagagta tggaagcggg attaggtatc
    51061 gttgcggtca tattaatgtt tatcgttggt gtttggatgc ataaacgttc gaatgcgaaa
    51121 cgctggaatg ctatgattaa aaatatgtat gccaatgcga ttagtaatgg taatttggta
    51181 ttgttagcga cgattggttt aatatctgtg ttgcgtgaag gtgttgaggt tatcattttc
    51241 tatatgggga tgataggcga attagcgacc aaagattttg tcataggtat cgccttagcc
    51301 atcgtgatat taattatctt tgcgttatta tttagattta tcgtcagatt gatacctata
    51361 ttctatatat ttagggtatt gtcgaacttt atttttatta tgggattcaa aatgcttggc
    51421 gtaagtattc aaaagttaca attattaggt gcgatgccaa gacatgttat cgaaggattc
    51481 ccaacgatta actggttagg attctatcca agttatgaac cattgatagc acaagctgcc
    51541 tatattatag tagttgctat cttaatcttt aaatttaaaa aataaaaaac aggccgagtg
    51601 cctgtttttt tgttgttgca ttgcggatta tcgtagcatt gcagatatcg tggtattgtt
    51661 gagatgcgga tatcacttca ttgttggttc tcgttgtatt gcagatatca cggtttaatc
    51721 cgatgctgta ttctaattat cgcagtgtta gttaggtgca gtattgatat aatctgaggt
    51781 gctacattgt tcttttattg gctattgtga tgttgctgca ttgtgagtat tgcagtattg
    51841 attcgtataa ggtcattgcg acgataagtc atttcgactg gtgaacttaa caatgaacat
    51901 actgaactca aagagcaata tgagtggcaa tgtgagtaat atatttaatg ttatgtctgg
    51961 tggtgcaatg atactcgcca gcacaaagca agcaaaataa atatatttac gataatgttt
    52021 caaagaatat gtatctataa gatcgaattt tgcaagacct atgaataata ttggtaattg
    52081 aaatagaatg ccaaatgtga ataaccaacg tatgagttca atcaaatatg ctttaaagcc
    52141 aatgacaggc gaaatgttta aagttaatga taattttaac gcgaattgaa tgatcattgg
    52201 aaagccaaca taaaatgcaa aagcgacgcc agcacagaat aataacacgc taaaaaacta
    52261 tatttataaa taaattgacg ttcattatta tgtaatccag gtgcaatgaa tgcccacaat
    52321 tgataaaaca tgactggtga aatgaaacaa aacgcgatga aaaatataat catcacgtat
    52381 atttggatca tttctgtgaa tgaaaatgca tgtaaggaca catgtgcacg ggtaatatac
    52441 gttatgaatg gtgtcatcca ccaaaatgat gaaatatata cgacgatgac cgtaatgacg
    52501 aacgacaata aaatttttac taaccgatgg cgtagttcgc taaagtgaac cattaatgta
    52561 tcatcagtac tattgctctc gctgttgctt cgattcctta ctgggtgtat cgtgagactc
    52621 tttatctaaa tcttctgttg cagatttaaa ttcttttaaa gtagaaccga tagcacgacc
    52681 aaattgtggt aattttttcg gaccaaaaat aattaaagcg ataatgctaa tgacgacaag
    52741 acttgttgga cctgtgatgc ctaaaataaa agtgttagtt atcatgataa tcaacctcac
    52801 tcataagtag tatatagtat ttactttata ccgaacgata atgattatca acaatatttt
    52861 atataataat taaaatattg ttataaacac aacttactga ggtattaatt ctgaaataat
    52921 agcttgttct ttcaagtcga tataagtaca agtaccagct tgaatatcga tatgccattt
    52981 ataaatgcca gcttcagcca tttcatcaca aaatgtttcg aaatctgttt gtccttgttg
    53041 gtgtctagtt aatacatctt gaacgataga ttgatttgaa cttggagcga caggatgatt
    53101 actttttaca gatgaagtaa caatatcatc ttctgattga tgtacgtatg ttgcagtgcc
    53161 atcttgaata ttaacaatat tgtacgtcat acccatatcc ttaaatgctt tgaatagttt
    53221 tggaaaatcg acaccagtaa attgttgatg tgcttgttgg atttcagata atgtaaatgc
    53281 cataatagtc tcctttgaat ggtttgattt agtgaaaatg cgtgcatcat actaaaatta
    53341 gaatgctgtt aattatgcag gaaggtgtaa cgaaagtaaa caattatgac ttgtaggtgt
    53401 tgaggtgtgt gtttcaaaat gtcatacata ggtggacact tatatttttt tgtgctaatt
    53461 tgacaaatat agttgtcaaa aagacaaata tcatttacag tgtagttgag gagttgatgc
    53521 gatgtgcgta atcgattgaa agaattacga gcacgagatg gcttaaatca aacgcagctt
    53581 gccaaactag cgggtgtttc aagacaaacc atttcgctaa ttgagcgaaa caactttatg
    53641 ccatcggtat taactgcaat aaagattgct cgtatcttca atgaaaaggt ggaaactatt
    53701 tttattattg aggaggatga ggcatgaaaa tactaagata tatcggatat cttttactgg
    53761 gtggacttgt agggggtatc ataggtggaa ttttaggtaa ctttgatgga ttgggtattg
    53821 agaacttgac gtttgcgaca tataacaatg tcgttgtaat atcgattgtt gcgactatta
    53881 ttatcatatt ggtagaagcc attgttttga tgaatcaaag acgtgcattg aagtataagc
    53941 gacttgtaga tgaagaggta gatatcgatg caacagatca atatgaattg cttgcgaatc
    54001 gttatgtttt aaatggaagt atattaagca ttatacagac aattattgcc ttgttagtgt
    54061 tgcttatttt tgtggtagga caagctgaag caaatggaat actattcttt ttaatacctt
    54121 tttttgttag tggtattttc aatacacaat ttacgctgtt taatagaaga tttgatgaca
    54181 gaatgccaaa aattgcagat aagaattaca ctgaaaagcg attggaaata ttggatgaag
    54241 gtgaacgcca tatagaatta atagcattat ttaaaacata tgcgatcaac ttatccatat
    54301 tgatactagc cattattttt atagggtctt attcaattgc tacaggaatt aatcaaagct
    54361 ttagtttgct acttatcatt gctattttca tatataacgc ctttagttat ttattgaaga
    54421 gaagacgttt ttattaaaat taaacaaaga ggagcatgga tatgacaaca ttgttaaacg
    54481 tagatcgtgt gaacaaacaa tacaaagatt cggattttaa attacaagat gcatctttaa
    54541 cgattgctac taatgagaca gtaggattaa tcgggaaaaa tggctcgggt aaatcgacat
    54601 taattaatat tctagtaggc aatcgacata aagataacgg tagtattttg ttttttggag
    54661 aagaacatgc tatggatgat gtcgaatata aagaacatat aggtgtagtg tttgatgatt
    54721 tgagagtacc taataaattg acaattaaag atattgataa agtatttcaa tctatttata
    54781 cgagttggaa tagtcaaaaa ttctttgatt taatcaaata tttcgagtta ccactacaaa
    54841 ctaagattaa aactttttca agagggatgc gaatgaagat agctttaacg attgcgcttt
    54901 ctcatgatgt gaagctattg atcttagatg aagcaactgc aggtatggat gtttctggac
    54961 gagaagaagt aatggaacta ttagaagatt ttgtcgcgca aggtggaggc atcttaatat
    55021 catcgcatat ttctgaagat atagaacaat tagcggataa attagtgttt atgaaagatg
    55081 gacgaatgat tttaactgaa cagaaagata tacttttagc acaatatgga attgttacga
    55141 cagaagagaa agatgttgaa attcctaagc atttaatcat tgcatctaga ttatcgaagg
    55201 ggaaatatca aattttagtt aaagattatg aagaaattga aaatgcagaa cctttaaaac
    55261 acattgatga cgctacgaaa atcataatgc gaggtgaagt ataatgaaag gtatgttcct
    55321 aagtagtttt tatgcaacga gaaagcaaac atatatttat tttatagtcg ctatcatagc
    55381 tgcgggatac tttgcagtat ttaatccgtt gatgagttcg gcaatggctg gggttatgtt
    55441 aatcacacct attactgata atattaaaca tgaaaaagac tcaagatgga tgtattatgt
    55501 atctacttta ccggttaaac gtagtgatta tattaagtca tactttgcct tttatttaat
    55561 cttattcggt gcaagtttaa tgattggatt agttgtgact acgattgtga cccaaagtgt
    55621 gatgattggt attatgtcag gtttaatgag ttttggtatc ataggggcat actctatcat
    55681 tttcccattg acatttaaat ttggcgctga aaactctaat gtcattatga tatttgcatc
    55741 tatactacta cttttttctt tcgtggtctt tttctttata tatggtatgg ttagtggtgc
    55801 atctgcatta gaatttgaaa aaattagcac tggaggatgg ctagttgtca tagtatatgc
    55861 ggttatcggt atagttataa caagtgtttc ttatatattg tctattaaaa tttttaacaa
    55921 acaagaacta taatcaatat tcttggtgtg aagctagtca ttgaaataat atttagagca
    55981 ggtgataaat caatacgatt atcatctgct ctttttggtg tggaatgaaa tgcagggtga
    56041 taagtatagg tgacatatct agattgattt atttgttttg aggtggttat gttgtgtggg
    56101 aattatttcc ttttagatag tggggattag aggatatatg ttatttataa gtatcatttg
    56161 atgattgtat aggctaacga tttcctcgga aatatttaaa aacctcgatc atgtagcata
    56221 actgaagttt gtcacaaagt ataatgtgaa gttcgacact tttggattca gttcaaatac
    56281 tttgaccgag gtaaatacta tttatttatg tttattacgt agacgttgat ttttaaaata
    56341 aatcagcatg atgcagaagg ttgcaactga tactgagata atcattacat ggtcgtgtcc
    56401 cttaaataaa aggctgacaa tataagacat aacaagtatc cctagtgaat atgaaatata
    56461 cttcgcattt gtcaattcgt tatggaaata aggcgtgatt aaccataatc caatatagaa
    56521 tattaaaaca ctgatataca tcatattaat ttcaaacaag tcatttaatt tattgttatt
    56581 actaaaaaca attgcggcat taatcacacc taaagcgata ttgattaata gatgcgtata
    56641 cgataaacgg aaaccgatag atgacaattt atgattaatg taattttcag taatgatcca
    56701 atatacaccg aaaagactaa ttaaaatcat aaattggaat atataaatgt aactaaaatg
    56761 atcaatgcta aatgatgacg aagctaaacc aaccagtacc tcgccaaaga taataattgt
    56821 tagtaacgaa aaacgttcta ctaaatgcat catattaaca ggtgataata caagatattt
    56881 ctgaaatgga ataagtcctg tcgctgcaat gaatacgcct aaaaatccag ggatgtaatg
    56941 gatactttgt ggtagtacta atgacagaaa tgataaaaat gaaatcacaa aggctacggt
    57001 agcaaaggct tggcatgtac gcttatctcc ataatctaaa ccggtaccta tatgtaacaa
    57061 gtattgtaaa ccgatactta gatacataat tgcaacgcat aagaagaatg ggaagaatgt
    57121 tttttcaaag tccggatata ggctgttaga taggaagacc atgataaaca tattaaacat
    57181 cataaacgag acgtctttga atgtaacttg accaaatcga tttgtaaaaa atgtttgatg
    57241 agaccacatt aaccataaga acaaactcat gacgatgtat ttgaaaaata aatcagctga
    57301 aatggaacca ttttgtattg ttaaaatcac atgtgcaatt ttttgaatgg catagacgaa
    57361 aattaaatca aagaacaact catggaatcc tgcacgtttt tcagctaaat gttttggtgt
    57421 taatgcatta accat
//
LOCUS       DAPIDM010000005        49294 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000005 DAPIDM010000000
VERSION     DAPIDM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 49294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.005"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..189
                     /locus_tag="RJE42_000258"
     CDS             <1..189
                     /locus_tag="RJE42_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875714.1"
                     /translation="TVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLK"
     gene            1138..1959
                     /gene="cntK"
                     /locus_tag="RJE42_000259"
     CDS             1138..1959
                     /gene="cntK"
                     /locus_tag="RJE42_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDV5875715.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVETYVRKQQWNHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            1970..2788
                     /gene="cntL"
                     /locus_tag="RJE42_000260"
     CDS             1970..2788
                     /gene="cntL"
                     /locus_tag="RJE42_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDV5875716.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLINSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            2781..4082
                     /gene="cntM"
                     /locus_tag="RJE42_000261"
     CDS             2781..4082
                     /gene="cntM"
                     /locus_tag="RJE42_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDV5875717.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTYSNSTMCQRISVLAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEEDKALVTKFLEINRTLS"
     gene            4225..5823
                     /gene="cntA"
                     /locus_tag="RJE42_000262"
     CDS             4225..5823
                     /gene="cntA"
                     /locus_tag="RJE42_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDV5875718.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            5836..6771
                     /locus_tag="RJE42_000263"
     CDS             5836..6771
                     /locus_tag="RJE42_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875719.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            6768..7637
                     /locus_tag="RJE42_000264"
     CDS             6768..7637
                     /locus_tag="RJE42_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875720.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVMQS"
     gene            7634..8449
                     /locus_tag="RJE42_000265"
     CDS             7634..8449
                     /locus_tag="RJE42_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875721.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIVGLNPERLRVTGDITFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVCD"
     gene            8442..9191
                     /locus_tag="RJE42_000266"
     CDS             8442..9191
                     /locus_tag="RJE42_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875722.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            9203..10396
                     /locus_tag="RJE42_000267"
     CDS             9203..10396
                     /locus_tag="RJE42_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5875723.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFIVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PANSL"
     gene            complement(10620..11366)
                     /locus_tag="RJE42_000268"
     CDS             complement(10620..11366)
                     /locus_tag="RJE42_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875724.1"
                     /translation="MSNTNKHYIEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVFL
                     TFELVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASAK
                     YKWHILISFTLSIVLFLLLNHFDIFALNVDSFTVFDIYPLIREYGSISLIAIFSLTII
                     LNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILASS
                     ATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            11561..13138
                     /locus_tag="RJE42_000269"
     CDS             11561..13138
                     /locus_tag="RJE42_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDV5875725.1"
                     /translation="MTFLTVMQFIVNIIIIGFLLTVMVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEDLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSNPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(13306..13761)
                     /locus_tag="RJE42_000270"
     CDS             complement(13306..13761)
                     /locus_tag="RJE42_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV5875726.1"
                     /translation="MKKLCSLIVVALVCVIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            14000..15076
                     /locus_tag="RJE42_000271"
     CDS             14000..15076
                     /locus_tag="RJE42_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDV5875727.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDLGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLTNDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKKHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSVRLVTEIVRSLNDETYKNIMW"
     gene            15422..16084
                     /locus_tag="RJE42_000272"
     CDS             15422..16084
                     /locus_tag="RJE42_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV5875728.1"
                     /translation="MLLEIKDLVYEASDRVILNHINLKVDKGETIAIIGPSGSGKSTF
                     QKLICNLISPTSGTLYFKGKPYDDYEPEILRRHISYLMQQSDLFGETIEDNMIFPSVA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDINNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            16077..16853
                     /gene="fetB"
                     /locus_tag="RJE42_000273"
     CDS             16077..16853
                     /gene="fetB"
                     /locus_tag="RJE42_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV5875729.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFLISFVAIFVGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            17248..18435
                     /locus_tag="RJE42_000274"
     CDS             17248..18435
                     /locus_tag="RJE42_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5875730.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAVTYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(18497..19849)
                     /locus_tag="RJE42_000275"
     CDS             complement(18497..19849)
                     /locus_tag="RJE42_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDV5875731.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSATFTEHEDCLYLNIWKQHNDQSKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKLMRDYLDTDDVTSLSTDDILMLMEKLKQSRGPSKGLDLIYA
                     PIKTGYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTTHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(20147..21793)
                     /locus_tag="RJE42_000276"
     CDS             complement(20147..21793)
                     /locus_tag="RJE42_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDV5875732.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMKHLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSQKIF"
     gene            21974..22573
                     /locus_tag="RJE42_000277"
     CDS             21974..22573
                     /locus_tag="RJE42_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDV5875733.1"
                     /translation="MSEKQPNEQVEAFLNKESQWQEEYKYLRTLIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLEE
                     IQARREEIKHYIAEAIKAEKAGKKVKMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            22620..22717
                     /locus_tag="RJE42_000278"
                     /pseudo
     CDS             22620..22717
                     /locus_tag="RJE42_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22797..22916
                     /locus_tag="RJE42_000279"
     CDS             22797..22916
                     /locus_tag="RJE42_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875734.1"
                     /translation="MDVYPPVTMDACALLQKFDFCNNISHFRHFFDIKQPIER"
     gene            23243..24469
                     /locus_tag="RJE42_000280"
     CDS             23243..24469
                     /locus_tag="RJE42_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875735.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAADQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDRDNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTTSSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            24466..25101
                     /locus_tag="RJE42_000281"
     CDS             24466..25101
                     /locus_tag="RJE42_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875736.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDIELPLALPLILGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLSLVEKWVVPKGLKVSG"
     gene            25118..26059
                     /locus_tag="RJE42_000282"
     CDS             25118..26059
                     /locus_tag="RJE42_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5875737.1"
                     /translation="MKKIKYIVVVLVLSLAVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGV
                     LKEAPIKDPKKAMIATQQGFKQKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            26059..26754
                     /locus_tag="RJE42_000283"
     CDS             26059..26754
                     /locus_tag="RJE42_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875738.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            27048..28448
                     /locus_tag="RJE42_000284"
     CDS             27048..28448
                     /locus_tag="RJE42_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5875739.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            28674..29609
                     /locus_tag="RJE42_000285"
     CDS             28674..29609
                     /locus_tag="RJE42_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV5875740.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            29929..30780
                     /locus_tag="RJE42_000286"
     CDS             29929..30780
                     /locus_tag="RJE42_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV5875741.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(30887..32386)
                     /locus_tag="RJE42_000287"
     CDS             complement(30887..32386)
                     /locus_tag="RJE42_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase FLP"
                     /protein_id="HDV5875742.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNNKSKNDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLTLTTTFIALFSMLITILLVLHTTTIKKP"
     gene            32618..34447
                     /locus_tag="RJE42_000288"
     CDS             32618..34447
                     /locus_tag="RJE42_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDV5875743.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVHNVEVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(34586..36664)
                     /locus_tag="RJE42_000289"
     CDS             complement(34586..36664)
                     /locus_tag="RJE42_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDV5875744.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIE
                     HFKNESKKDKNDTNYRPVLNQYFGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(36802..38211)
                     /locus_tag="RJE42_000290"
     CDS             complement(36802..38211)
                     /locus_tag="RJE42_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5875745.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            38498..39304
                     /locus_tag="RJE42_000291"
     CDS             38498..39304
                     /locus_tag="RJE42_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV5875746.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKNLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVAKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGTSDQNLSKIA
                     AEQVKKDLLEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(39367..40023)
                     /locus_tag="RJE42_000292"
     CDS             complement(39367..40023)
                     /locus_tag="RJE42_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDV5875747.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            40136..40609
                     /locus_tag="RJE42_000293"
     CDS             40136..40609
                     /locus_tag="RJE42_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV5875748.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(40732..41943)
                     /locus_tag="RJE42_000294"
     CDS             complement(40732..41943)
                     /locus_tag="RJE42_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDV5875749.1"
                     /translation="MQSSSSKTKQFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILASGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTPKEELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSMMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(42131..42790)
                     /locus_tag="RJE42_000295"
     CDS             complement(42131..42790)
                     /locus_tag="RJE42_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875750.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNSGG
                     N"
     gene            complement(42850..43992)
                     /locus_tag="RJE42_000296"
     CDS             complement(42850..43992)
                     /locus_tag="RJE42_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDV5875751.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKICTGKSVNQVPGSGAAGGMGAALLAFFDTTLTKGIDIV
                     FDITNFHQRIKDADLIITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            44259..44645
                     /gene="gtxA"
                     /locus_tag="RJE42_000297"
     CDS             44259..44645
                     /gene="gtxA"
                     /locus_tag="RJE42_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDV5875752.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQLVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            44779..44886
                     /locus_tag="RJE42_000298"
     CDS             44779..44886
                     /locus_tag="RJE42_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDV5875753.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            45590..47353
                     /locus_tag="RJE42_000299"
     CDS             45590..47353
                     /locus_tag="RJE42_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875754.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGSSVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQLLILEELALSSRFMEPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            47378..49111
                     /locus_tag="RJE42_000300"
     CDS             47378..49111
                     /locus_tag="RJE42_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875755.1"
                     /translation="MFQITFKILNWMRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILIIVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQSVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGIIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATSSIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
BASE COUNT        16660 a         7236 c         9341 g        16057 t
ORIGIN      
        1 acagtaagta tagagaacat aagtcatagt gagcttttga atttaataga aaatacgatt
       61 ccagaagatc gagagattaa cacgtttgta aaaaataaaa taataattgg ctttgctaat
      121 aattatttcc ctgaattaca agtattagca aacgaaataa aatctgatat ggctagttca
      181 ttaaaataat gatattttta tttgaatttt taaagctttg tacgaaattg tacgaagctt
      241 ttttggtgcg cattgcatgg agaacaactt gacgatgaaa atccgttaca ggattggtaa
      301 taggaaatgt tagcgaaaga caaggatgtc cattgtagat taacaaaagg acgtttccac
      361 tagtgtgggt tattctcact aaaccaatac gcagagacaa cttacgtaaa attttgaact
      421 gacgagaacg gaacttctac tcaattattg ataaaaattt tcaaaagaac ttgaatgtgc
      481 tgagaatacg aagtttatgg aaggattatc aaaatataaa tgtgctttca tttacaacct
      541 ttattgacaa tgattctcaa ctaatatagt atataatcaa atcgtaataa ttacgatttg
      601 ttttctgcaa cttttttgaa gttttagttg aggtgaaaac agtaaaagca tctaagtgaa
      661 tgtagttaac ggacaactgc attcgcttgt agagccacaa gaagcaactt taaataaggt
      721 ttacggttgc atgttgatgc aacaaccgat tactaagtca tgctttccac tttgcgggtt
      781 agcatgactt acctaataga tgggaaacta ttaggttcat cttctaaaaa attacaggta
      841 tagaggaata cagttgtttg cttcgcaaca actgcataag agcctctagt aattaaaatt
      901 acagttttag aggaatacag ttgcttgctt cgcaccaact gcataagagc ctgagacata
      961 aatcaatgtt ctatgctcta caaagttata atggcagtag ttgactgaat gaaaatgcgc
     1021 ttgtaacaag cttttttcaa ttctagtcaa ccttgccggg gtgggacgac gaaataaatt
     1081 ttacgaaaat atcatttctg tcccactccc taaaaaatac atctaaagga gtgtcgtatg
     1141 aatcggcagg ttatagaatt ttctaagtat aatccttcgg ggaatatgac gatacttgtt
     1201 cattcaaaac atgatgctag tgaatatgca tctatcgcca atcagttgat ggccgcaaca
     1261 catgtatgtt gtgaacaggt aggctttata gaatcaacac aaaatgatga tggcaatgat
     1321 ttccatctag ttatgagcgg taatgaattt tgcggtaatg cgacgatgtc atatatacat
     1381 catttgcaag aaagtcattt gcttaaagac caacagttta aggtgaaggt gtctggctgt
     1441 tcggatttag tgcaatgcgc aattcatgat tgccaatact atgaagttca aatgccacaa
     1501 gcccatcgtg ttgtgccaac aacaattaat atgggtaatc attcatggaa agcaatagaa
     1561 attatttatg aaacatatgt acattatgtg attccagtta aacaagtaac aacagaaatt
     1621 caacatttgg ttgaaacgta tgtgcgtaag caacaatgga atcacaaata taaaacagta
     1681 ggtatgatgc tttttgatga acaacgtcaa tttttacagc cattaatcta tataccagaa
     1741 atccaaagtt taatttggga aaatagctgt ggttctggta cagcatcaat tggggttttt
     1801 aataattatc aacgtaatga cgcatgcaaa gattttacag tacatcagcc agggggcagt
     1861 attttagtga catcaaagcg atgtcaccaa ttgggatatc aaacttcaat taaaggacag
     1921 gttacaactg tagctacagg aaaagcatat atagaataag gagcctacaa tgaataactt
     1981 taataatgaa atcaaattga tattacaaca atatttagaa aagtttgaag cgcattacga
     2041 gcgtgtatta caagacgatc aatatatcga agcattagaa acattgatgg atgactatag
     2101 tgaatttatt ttaaatccta tttatgaaca acaatttaat gcttggcgtg acgttgaaga
     2161 aaaagcacaa ttaataaatt cactgcaata tattacagcg cagtgtgtta aacaagtgga
     2221 agtcattaga gctagacgtc tattagacgg acaggcgtct accacaggtt actttgacaa
     2281 tatagaacat tgtattgatg aagagtttgg acaatgtagt atagctagca atgacaaatt
     2341 attgctagtt ggttcaggtg catatccaat gacgttaatt caagtagcaa aagaaacagg
     2401 tgcttcagtt atcggtattg atattgatcc acaagccgtt gacctaggac gcagaatcgt
     2461 taacgtctta gcaccaaatg aagatataac aattacggat caaaaggtat ctgaacttaa
     2521 agatatcaaa gatgtgacgc atatcatatt cagctcgaca attcctttaa agtacagcat
     2581 tttagaagaa ttatatgatt taacaaatga aaatgtcgta gttgcaatgc gctttggtga
     2641 tggcatcaaa gcaatattta attatccgtc acaagaaaca gcggaagata agtggcaatg
     2701 tgtgaataaa catatgagac cacagcaaat ttttgatata gcactttata aaaaagcagc
     2761 tataaaggta ggtattacgg atgtctaaat tattaatgat aggcactggt ccggtcgcaa
     2821 tccaattagc gaatatttgc tatttaaaat cagattatga gattgatatg gttggacgtg
     2881 cctcaacatc agaaaaatca aaacgcttat atcaagcgta taaaaaagag aaacaatttg
     2941 aagtcaaaat acaaaacgag gcgcatcaac atctggaagg taagtttgaa attaatcgtt
     3001 tgtataaaga cgttaaaaac gttaagggtg aatacgaaac ggttgtcatg gcatgtacag
     3061 cagatgctta ttatgacaca ctacagcaat tgtcgttaga aactttgcaa agtgtcaaac
     3121 atgtcatttt aatttcaccg acatttggat cgcaaatgat tgtggaacaa tttatgtcta
     3181 agttcagtca ggatatcgaa gtgatttcat tttcaactta tcttggcgat acacgtattg
     3241 ttgataaaga agcacctaat catgtgttga caacaggtgt aaaaaagaaa ttgtacatgg
     3301 gttcgacata ttcaaactca acaatgtgtc aacgaatctc tgttttagct gagcaattga
     3361 agattcaatt agaagtcgtt gaatcgccac tgcatgctga aacacgtaac agttcgcttt
     3421 atgtgcaccc accactattt atgaatgact tttcattgaa agccattttc gaaggaacgg
     3481 atgtaccggt ttatgtgtat aagttatttc ctgaaggacc gataacgatg acactaatcc
     3541 gtgaaatgcg tctaatgtgg aaagaaatga tggctatttt acaagcattt agagtgccgt
     3601 cagtcaacct acttcaattt atggtgaagg aaaattatcc ggtacgtcct gaaactttgg
     3661 atgaaggtga tattgagcat ttcgaaatct tgccagatat cttacaagaa tatctacttt
     3721 atgtaagata taccgcaatc ctcattgatc cattttcaca gccagacgaa aacggacatt
     3781 actttgattt ttcagctgta ccatttaagc aagtctataa aaatgaacag gatgttgttc
     3841 aaattccaag aatgccaagt gaagattatt acagaacggc gatgattcag catattggga
     3901 aaatgctagg tatcaaaacg ccaatgattg atcagttcct aactcgctat gaagcaagtt
     3961 gccaggcgta caaggatatg catcaagatc aacacttatc ttctcaattt aatacaaatt
     4021 tatttgaaga agataaggca ctcgtcacaa aatttttgga aatcaataga acgctttcat
     4081 aataagggtt tgaagtttta taatagaaaa aaattattga attatgtttg acatttacat
     4141 aaaaataagc aaataattga gaaaaataat cattacgatt tgattaagta atgcaactta
     4201 tcaatttaga aagaggaaaa gcaaatgaga aaactaacta aaatgagtgc aatgttactt
     4261 gcatcagggc taattttaac tggttgtggc ggtaataaag gtttagagga gaaaaaagaa
     4321 aacaagcaat taacgtatac gacggttaaa gatatcgggg atatgaatcc gcatgtttac
     4381 ggtggatcga tgtctgctga aagtatgata tacgagccgc ttgtacgtaa cacgaaagat
     4441 gggattaagc ctttactagc taaaaaatgg gatgtgtctg aagatgggaa gacatataca
     4501 ttccatttga gagatgacgt taaattccat gatggtacgc catttgatgc tgacgcagtt
     4561 aagaaaaata ttgacgcagt tcaagaaaac aaaaaattgc attcttggtt aaagatttca
     4621 acattaattg acaatgttaa agttaaagat aagtacacgg ttgaattgaa tttgaaagaa
     4681 gcatatcaac ctgcattggc tgaattagcg atgcctcgtc catatgtatt tgtgtctcca
     4741 aaagacttta aaaacggcac aacaaaagat ggcgttaaaa agttcgatgg tactggtcca
     4801 tttaaattag gtgaacacaa aaaagatgag tctgcagact ttaataaaaa tgatcaatac
     4861 tggggcgaaa agtctaaact taacaaagta caagcaaaag taatgcctgc tggtgaaaca
     4921 gcattcctat caatgaaaaa aggtgaaaca aactttgcct tcacagacga tagaggtaca
     4981 gatagcttag acaaagactc tttaaaacaa ttgaaagata caggtgacta tcaagttaag
     5041 cgtagtcaac ctatgaatac gaaaatgtta gtggtcaatt ctggtaaaaa agataacgct
     5101 gtcagtgaca aaacagtcag acaagcgatt ggtcatatgg taaacagaga taaaattgcc
     5161 aaagaaattt tagatggtca agaaaaacca gcaactcaat tatttgcgaa aaatgtaaca
     5221 gacattaatt tcgatatgcc aacacgtaag tatgacctta aaaaagcaga atcattatta
     5281 gatgaagctg gttggaagaa aggtaaagac agcgatgttc gtcaaaaaga tggtaaaaac
     5341 cttgaaatgg caatgtacta tgacaaaggt tcttcaagtc aaaaagaaca agcagaatac
     5401 ttacaagcag aatttaagaa aatgggtatt aagttaaaca tcaatggcga aacatcagat
     5461 aaaattgctg aacgtcgtac ttctggtgat tatgacttaa tgttcaacca aacttgggga
     5521 ttattgtacg atccacaaag tactattgca gcatttaaag cgaaaaatgg ttatgaaagt
     5581 gcaacatcag gcattgagaa caaagataaa atatacaaca gcattgatga cgcatttaaa
     5641 atccaaaacg gtaaagagcg ttcagacgct tataaaaaca ttttgaaaca aattgatgat
     5701 gaaggtatct ttatccctat ttcacacggt agtatgacag ttgttgcgcc gaaagattta
     5761 gaaaaagtat cattcacaca atcacagtat gaattaccat tcaatgaaat gcagtataaa
     5821 taaaggagcg tttagatgtt caaatttatc ttaaaacgta ttgcgctcat gtttccattg
     5881 atgattgtag taagttttat gacatttcta ttgacgtata ttacaaatga aaatccagct
     5941 gtgacaattt tacatgcaca aggaacgcca aatgtaacac cagagttgat tgcagaaacg
     6001 aatgagaagt acggtttcaa tgatccatta ttaattcaat ataaaaattg gttacttgaa
     6061 gcgatgcaat ttaattttgg tacaagctac attacaggtg atcctgttgc tgaacgcatt
     6121 ggtccagcat ttatgaatac attgaaatta acaataattt caagtgttat ggtaatgatt
     6181 acatcaatta ttttaggtgt agttagtgca ttaaaaagag gaaagttcac tgatcgtgcg
     6241 atacgttcag tggctttctt tctaactgca ttaccatcat attggatagc ttcaatactt
     6301 attatttacg tttcagtgaa gttaaacata ttgccgactt ctggattaac aggtccagaa
     6361 agttacatat tgccagtgat cgttattacg attgcctatg ctggtattta ctttagaaat
     6421 gttagacgtt cgatggtgga acaattaaat gaagattatg tactttattt aagagcaagc
     6481 ggtgtgaaat ctatcacatt aatgttgcat gtgttgcgta atgctttaca agttgcggta
     6541 tcaatctttt gtatgtctat accaatgata atgggtggac tagttgttat cgagtatatc
     6601 tttgcatggc ctggactagg tcaattaagt ttaaaagcaa tacttgaaca tgattttcca
     6661 gtcattcaag catatgtatt aattgtagcg gtattattta ttgtatttaa tacattagca
     6721 gatatcatta atgcgctatt aaatccaaga ctaagggagg gcgcacgatg ataattttaa
     6781 aacgattatt acaagataaa ggtgcagtga ttgctttagg cattattgta ttatatgtct
     6841 ttttaggatt agcagcacca cttgtgacat tttatgatcc taaccatatc gatacagcaa
     6901 acaaatttgc tggcatgagt tttcaacatt tattaggcac agaccattta ggtagagata
     6961 ttttaactag gttaatttat gcgattagac caagtttgtt atatgtcttt gttgcgctat
     7021 ttgtttctgt acttattgga tctattttag gattcttatc aggatatttc caagggtttg
     7081 ttgacgcctt aatcatgcgt gcgtgtgatg ttatgttggc attcccaagt tatgttgtaa
     7141 cgttagcatt aattgcattg tttggtatgg gtgccgaaaa tattatcatg gcatttattt
     7201 tgacgcgttg ggcatggttc tgtcgtgtta tacgtacaag tgttatgcag tacactgctt
     7261 ctgaccatgt aagatttgct aaaacaatcg gtatgaatga tatgaaaatt attcacaaac
     7321 atattatgcc gttaacatta gcagatattg ctatcatctc tagtagttcg atgtgttcaa
     7381 tgatcttgca aatatctggc ttttcatttt taggattagg tgtcaaagcg cctactgcag
     7441 agtggggtat gatgcttaac gaagctagaa aagtgatgtt tacacatcct gaaatgatgt
     7501 ttgcgccagg tattgccatt gtgattatag tgatggcatt taacttctta tccgatgctt
     7561 tacaaattgc cattgatccc cgcatctctt ctaaagataa acttcgttct gtgaaaaaag
     7621 gagtgatgca atcatgacat tgttaacagt taagcatttg acgattacag atacctggac
     7681 agatcaacca ctcgtgagtg atgtgaattt tacattaact aagggtgaaa ctttaggcgt
     7741 tattggagaa agtggtagcg gtaaatcaat tacatgcaaa tcgattgtag gtttaaatcc
     7801 agaacgatta cgcgtaacag gtgatataac ctttgatggt acatcaatgt tgtcattatc
     7861 cgaatcgcaa ctgaaaaagt accgtggtaa agatattgcg atggtcatgc aacaaggtag
     7921 tcgtgccttt gacccatcaa ctactgtcgg taaacaaatg tttgagacta tgaaagtaca
     7981 tacgtcaatg tctacacaag aaattgaaaa gacattgatt gaatatatgg attatttaag
     8041 tttgaaagat cctaaacgta tattaaaatc atacccttac atgttatcag gaggaatgtt
     8101 acagcgattg atgattgctt tagcgttagc tttgaaacca aagttaatca ttgctgatga
     8161 gccgacaacg gctttagata caattacaca atatgatgta ctggaagcat ttatagatat
     8221 taaaaaacac tttgactgtg cgatgatttt catttcacat gatttaacgg ttattaacaa
     8281 gattgcagac cgtgttgttg tgatgaaaaa tggtcaactg attgagcatg gtacgcgtga
     8341 atcagtcttg catcatccag aacatgttta tacgaagtat ttattatcaa ctaagaagaa
     8401 gattaatgat cattttaaac atgtgatgag gggtgatgta tgtgattaaa attaaagatg
     8461 ttgaaaagtc atatcaaagc gcacatgttt ttaagcgtcg tcgaacacct atcgtgaaag
     8521 gtgtgtcatt tgagtgtcca atcggtgcga cgattgcgat tatcggagaa agtggtagcg
     8581 gtaaatcgac gttgagtcgt atgatattag gtattgagaa accggataaa ggttgtgtaa
     8641 ccttaaatga tcaaccgatg cataagaaga aagtgagacg tcatcaaatt ggtgctgtat
     8701 ttcaagatta tacgtcatca ttacatccat ttcagactgt tagagaaatc ttatttgaag
     8761 tgatgtgtca atgtgatgga caacctaaag atgttatgga agtccaagca attacattgt
     8821 tggaagaagt cggtctatct aaggcataca tggataaata tcctaatatg ttatcaggtg
     8881 gagaggcgca gcgtgttgcg attgcgcgtg caatatgtat taaccctaaa tatattttgt
     8941 ttgatgaagc cattagttca ctcgacatgt caattcaaac acaaatatta gacttattga
     9001 ttcatttacg tgaaacgcgt cagttgagtt atatttttat cacacatgat attcaagctg
     9061 ccacgtattt atgtgatcaa ttaattattt ttaaaaacgg aaaaatagaa gaacaaattc
     9121 cgacaagcgc attgcataaa agtgacaatg cttatacaag agaattaata gaaaaacaac
     9181 tatcattcta aggagtgaga taatgaaagg tgcaatggct tggccctttt tgagattata
     9241 tatattaaca ttgatgttct ttagtgccaa tgcaatctta aacgtgttta tacctttacg
     9301 agggcatgat ttaggcgcaa cgaatacggt tatcggtatc gttatggggg catacatgtt
     9361 aacagcaatg gtatttcgac catgggcagg acaaattatt gctcgtgtcg gtcccattaa
     9421 agtattaaga attattttga ttatcaatgc catagcttta attatttatg gttttactgg
     9481 cttagaaggt tatttcgtag cacgtgttat gcaaggtgtg tgtacggcat tcttttctat
     9541 gtctttacag ctaggtatta ttgatgcatt accagaggaa catcgttctg aaggtgtatc
     9601 attgtactcg ctattttcaa cgattccaaa cttaatcgga ccattagttg ccgtaggtat
     9661 ttggaatgca aataatattt cactatttgc aattgtcatt atctttatcg cattaacaac
     9721 aacattcttt ggctatcgcg tgacctttgc tgaacaggaa cccgatacgt cagataagat
     9781 tgaaaaaatg ccgtttaacg ctgtaactgt ttttgcgcaa tttttcaaaa ataaagagtt
     9841 gttgaacagt ggtattatca tgattgttgc atcgattgta tttggtgcag ttagtacatt
     9901 tgtaccgtta tacacagtga gtttaggatt cgcgaatgcg ggaatctttt tgacaataca
     9961 ggccatcgca gttgttgtgg caagatttta cttaaggaaa tacattccgt cagatggtat
    10021 gtggcatcct aaatatatgg tatctgtact atcattatta gtgatcgcgt catttatagt
    10081 ggcatttggt ccgcaagtag gtgcaattat tttctatggc agtgcaatat taataggaat
    10141 gacacaagca atggtgtatc caacattaac atcatactta agtttcgtct taccaaaagt
    10201 aggtcgtaat atgttgttag gtttatttat tgcctgtgca gacttaggta tatcgttagg
    10261 tggcgcattg atgggaccta tttccgactt agtaggattt aaatggatgt atctaatttg
    10321 tggtatgtta gtcattgtaa taatgattat gagtttattg aaaaaaccaa caccacgccc
    10381 agcaaatagt ctttaatgaa gtgaattaaa gcatattaag ttaataaata tttaaatttt
    10441 aaaaggtata ttgagcatgg cgattcatgt gcttcatgct aggacatgaa acattctatg
    10501 tggctcgttt ttagaacgac agtatatcta aataaagcac gcttaaaagt gagttgtgat
    10561 actgactttt aagtgtgctt tttgatgtgt atggataatg gataatactc tgaaaaaatt
    10621 tacatgaaaa tatgcaaaac gagtataact gctaattgat agaaatagct caccataaaa
    10681 ttacggtttg attttaaata taagtaagtc gcactactag ctagtatcaa tgctggaatg
    10741 aattcccacc atgtattaat gtatggataa tagtacagtg tttcaaggat aacggataat
    10801 actattgtaa tctttaaagg tattaatctg cttaattctt gaattaaaat atgacggaaa
    10861 ataagttgac aaatcaaagt atttaatata atggttaacg aaaatatagc tattaaactg
    10921 atggaaccat attctctaat gagcgggtaa atgtcaaaga cagtaaagga atctacattt
    10981 agtgcgaaaa tatcgaaatg atttaaaagt aaaaagagta cgatacttaa tgtaaatgat
    11041 ataagaatat gccatttata tttagcactg gcaacgattt gcgaacgtat cattggaata
    11101 aacgcatctt catgcatcag acgaaaaata gctagtgaaa taataactgc gagtaaatag
    11161 ctaatgttca ttgaaatagg aaaagagaaa ccccatgggg cttgttgagt gaatacagct
    11221 actaactcaa aagttaaaaa gacgataatg attggcaaga tgttaaccaa aaatatataa
    11281 aggaaaataa atccaatatc acgtttgaaa aaacgcgatt gttcggtagc gtattcttct
    11341 atgtaatgtt tatttgtatt tgacatagta tacctcttaa atagttgtat tatatagata
    11401 ctttagcaca tattactttg tattgtatgt tttatacatt aaaatttaaa atgaaaaaca
    11461 tatcataaaa ttgttttata aaatgaagcg cttccattgt gttttatttt gtaaggtgta
    11521 tcataaatag tgaattgaaa tttttggggg agggattgta atgacgtttc ttacagtcat
    11581 gcaatttata gttaatatta tcattatagg cttcctactt acggtcatgg ttatcggact
    11641 tatttggtta attaaagata aaagacaatc acaacatagt gtattaagga attatccttt
    11701 actagcacgt attagatata tttcagaaaa aatgggaccg gaattacgtc agtatttatt
    11761 ttctggggat aatgaaggga aacctttttc acgtaatgat tataaaaata tcgttttggc
    11821 tggaaaatat aactctcgta tgaccagctt cggtactact aaagattatc aagacggctt
    11881 ttacatacag aacacaatgt ttccgatgca acgtaatgag atttcagtag ataatacaac
    11941 attgttatca acattcattt ataaaatcgc gaatgagcgt ttatttagtc gtgaagaata
    12001 tcgtgtaccg acaaagattg atccgtatta cttaagtgat gatcatgcaa taaaattagg
    12061 tgaacattta aaacatccgt ttattttaaa acgtatcgta ggacaatctg gtatgagtta
    12121 tggagcctta ggaaaaaatg ccattacagc tttatctaaa ggtctagcaa aagcgggcac
    12181 ttggatgaat acaggtgaag gtggcttatc agaatatcat ttaaaaggta atggggatat
    12241 cattttccaa attggtcccg gtttgtttgg tgttcgtgat aaagaaggta attttagtga
    12301 agatttattt aaagaggtcg cacagttatc taacgtacgc gcatttgagc tgaagttggc
    12361 acaaggtgct aagactcgtg gtggtcatat ggaagctgaa aaggtaaacg aagaaattgc
    12421 taaaatccga aatgttgaac cttataaaac aatcaattca cctaaccgtt acgaatttat
    12481 tcataatgct gaagatttga ttcgttttgt cgatcagttg cagcaattag gtcaaaaacc
    12541 agtaggattc aaaattgtag taagcaaagt ttcagaaatt gaaacacttg tacgtacgat
    12601 ggtggaacta gataagtatc caagctttat tacgattgat ggtggtgaag gtggtactgg
    12661 tgcaacattc caagaattac aagatggtgt tggcttaccg ctgtttacag ctctacctat
    12721 tgtgtctggc atgttagaaa aatatggtat tcgagataaa gtgaaattgg cagcatctgg
    12781 taagttagtg acaccagata aaattgcgat tgcactaggt ttaggtgcag attttgttaa
    12841 tattgcacgt ggcatgatga ttagtgtcgg ttgtattatg agtcaacaat gtcatatgaa
    12901 tacatgtcct gtaggtgttg caacgacaga tgcgaaaaaa gaaaaagcat tgattgttgg
    12961 agaaaagcaa tatcgtgtca caaactatgt aacaagtttg catgaaggct tattcaatat
    13021 tgcagcagct gttggcgtat ccaatcctac agaaattact gctgatcata ttgtatatcg
    13081 aaaagtcgat ggtgaattgc aaacgataca tgattataaa ttaaaactca ttagttaact
    13141 tatttatttc gggaaaatga aagcagcgga ttttagcgtt actgcaaata attttatatt
    13201 agtagtggat gctggtcaca caagaatttc aaatattaaa gccctcagaa tatgaattaa
    13261 ggtttgtaac cttagtctta tctgagggca tttttatgtt ataaactatt tgtcgtccat
    13321 tttatctttt tcttttaagc ctctgtgctt taattgcttt tcaagttttt caaaactaat
    13381 atctttattt tctttagttg aaacaccaag acgcttattt aattttttca tgtcaacttc
    13441 tgtgtaatct atgtctaagt gttcaattgc ttttttatct ttatagtcta ctttgtattt
    13501 tacgccttta aggtctttga aaatactttc agatttggcg aataactttt tggcttcgtc
    13561 tttatccata cctagatcgt catatttaat tgtgttgatt gtagactgtt ttaaaacttt
    13621 atcatcttta tatgtgatag aagttagtac atgtttacca ctaacatcac cttcatatgt
    13681 tttggtttgt tctttaccac aagctgataa tgcaatgaca caaactaatg ctactacaat
    13741 taatgaacat aattttttca aagtcagtcg ccttctttcg atatttgtat tataaagaaa
    13801 ttataacatt tactaaaaaa tgatgttatt caaaaattta aattttgata atttttttgt
    13861 aaatatgagg tttttgaagc ggatttctca caaagttttt aaaattatta agcctgaaaa
    13921 atgataaagc ggtagggaac gtttttctga aagttagtga tacaatagtt ttaagttgaa
    13981 atacaggagg atgaataaca tgaatcagtc agtcaaatta cttaaacatt taacagatgt
    14041 aaacggcatt gctggttatg aaatgcaagt taaagaagca atgcgtaact atatagagcc
    14101 tgtcagtgat caaattattg aagataattt gggtggcatt tttggaaaga aaaatgctga
    14161 gaatggtcaa tactcaatta tgatttctgg tcatatggat gaagttggtt ttatggtaac
    14221 aaagattgat aaacatggtt ttatttcatt tacgccagtt ggtggatggt ggaatcaagt
    14281 catgctatct caaaaagtaa cgattacaac agatttgggc aaagaaatta gaggtatcat
    14341 cggttctaaa ccgccgcatg tcttaacgcc tgaagaacgt aaaaagccga tggaaatcaa
    14401 aaatatgttt atagatattg gtgttagtag caaggaagaa gctgaagaag ctggcgttga
    14461 agtaggcaat atggttacgc catatagtga atttgaagtg cttacaaatg ataaatattt
    14521 aactgcgaaa gcatttgata atcgctatgg ctgtgcatta gctgttgagg tattaaaacg
    14581 tttaaaagat gaaaatattg gcattaactt atacagtggt gccacagtgc aagaagaagt
    14641 tgggttacgt ggtgcgaaag tggcagcgaa tacgattaaa ccagatttgg cgatagccgt
    14701 cgatgtgggt attgcttatg ataccccagg tatgtcaggt caaacgagcg atagtaaact
    14761 aggcggtggt ccagttgtta ttatgatgga tgctacaagt attgcacacc aaggtttgcg
    14821 caagcatatt aaagatgtag ctaagaaaca taacatcgaa gtacaatggg atacgacacc
    14881 aggtggaggt acagatgcgg gaagtattca tgtcgcaaat gaaggtattc caacgatgac
    14941 aataggtgtt acgttacgat acatgcactc taatgtatcg gtactcaatg tagatgatta
    15001 tgaaaattct gtccgtcttg ttactgaaat tgtccgttca ttgaatgatg aaacttataa
    15061 aaatatcatg tggtagtcaa attcataaat aatagagaaa gtctttaata tgatgtcatg
    15121 caagaactaa gttagatgct tagttagcat gagatgtcat gttaaaggct ttttattttt
    15181 ataaagatga gactaagtca ctgcagtata cttcaatttt cacataaata ttaaaaaaat
    15241 tttccagaat gaaatggcac taaatttttc aaaaatggga atactatatt tgatatgttt
    15301 tattagagcg attttaaggg gtaataagca ttgactaggt cgacttatag gtaagcatgc
    15361 tattcaagaa aaggattacc ctattatatc gaaataactc atgatacgag gaggggtaaa
    15421 tatgttgtta gagataaaag atttagttta tgaggcaagt gatagagtca tactaaatca
    15481 tattaattta aaagtagata aaggtgaaac cattgcaatt attggtcctt caggtagtgg
    15541 taaaagtaca tttcaaaaat taatatgtaa tttaattagc ccaaccagtg gaacgcttta
    15601 ctttaaaggt aaaccatatg atgattacga accagaaata ttacgtcgac acattagcta
    15661 tttaatgcaa caaagtgatt tatttggtga aacgattgaa gataatatga tttttccgtc
    15721 cgttgcgcgt aatgataaat ttgataagaa gcgtgccaag caattaatta aagatgtagg
    15781 tttaggacac tatcaattaa gttctaaaat agaacatatg tcaggtggcg agcgtcaaag
    15841 gatcgcgatt gcacgccaat taatgtatac cccagacatc cttctattag atgaatcaac
    15901 aagtgcgtta gatattaata ataaagaaaa aatagaaaat atcattttta aattagcaga
    15961 tcaaggcgtg gcaattatgt ggattaccca cagcgatgac caaagtatgc gacactttca
    16021 aaagcgtata acaattgttg atggtcaaat ttctaataca gaggagttga accaacatga
    16081 gtaatacagc attaggactg acggcattgc tattagttat tccaattatt atttcttata
    16141 aagaaggtct acatattatt aaagatttga ttgtggcgac acttcgtgcg gtcgtacaat
    16201 taatcatttt aggattctta ctgcactata tttttaaaat taatgacaaa tggctattag
    16261 tattatgtgt atttgtcatt attgtaaacg catcatggaa tacgattagt cgatcatcac
    16321 ctgtcatgca ccatgtcttt ttgatttcct ttgtggctat ctttgttgga acagcattac
    16381 cgcttgcagg tactattgcg acaggggcca ttcaatttac agccaatgaa gttataccta
    16441 tcggcggcat gcttgcaaat aatggcttga ttgcaattaa tttagcttac cagaatttag
    16501 atcgtgcatt cgtacaagat ggtactaata ttgaatctaa attatcactt gcagctacac
    16561 ctaaattggc ttctaaaggt gcaatacgtg aaagtattcg tttagctata gtgccaacaa
    16621 ttgattcggt taaaacatat gggcttgtgt cgattcctgg tatgatgaca ggcttaatta
    16681 ttggtggcgt accaccttta caagcgatta aatttcaatt gttagtcgtg tttattcata
    16741 caactgcgac cattatgtct gctttgattg cgacatattt aagctatggt caatttttca
    16801 atgcaagaca tcaattagta gcaagaaata cggatgttaa gagtgagtca tgatagattt
    16861 taccgcatca gatttagaca ttagttttaa ttggaaatga agtgacacgc acatatagta
    16921 atgttattat ttagctcagc gaaaatattt ataaaggcac gcattctttg tagtcagtta
    16981 tctgttctga catataaagc gtgcgtgctt ttttggagtc attgttgaaa ctgaagtaat
    17041 tatacataat tattaaatga catacttgtg ttaatttttc aaatactgaa aaacaatttc
    17101 aataattttc caattaagca cagaaaatta aagcaaaata ttatataata gaacggttat
    17161 atataaaatt gtgttgcaca cattttttaa taaatcgtta ttctaaggga aatgaatatc
    17221 ggaaattttg ttgaaaggag ttttaaaatg tcaatcatgc gactatttac attcatttta
    17281 agtattttta tcgtaggaat ggttgaaatg atggttgcag gaattatgaa cttgatgagt
    17341 caggacttac atgtatcaga agctgtcgtt ggtcaattag tgacaatgta cgctttaaca
    17401 tttgcgatat gtggacctat tcttgttaaa ttaacgaacc gtttttcatc aaggcctgta
    17461 ttattatgga cattacttat atttatcatt ggtaatggca ttattgctgt agcgccaaat
    17521 ttttcaatat tagtagttgg tagaattatc tcatctgcag cagcagcact aattatcgta
    17581 aaagtattag ctattacagc gatgttatca gcacctaaaa atcgtggcaa aatgattgga
    17641 cttgtctata cagggtttag tggtgccaat gtttttggtg taccaattgg aacggttatc
    17701 ggcgatttag taggttggcg ctatacattt ctattcttaa ttattgtgag tattattgtt
    17761 ggcttcttaa tgatgatcta tttaccgaag gatcaggaaa tacaacgagg ccctgtgaat
    17821 catgaggcac catctcatga aaatcatgtt acttcgaaaa tattaagacc tgctgaagta
    17881 gcaaaatatt taattattac gtttttagta ttgattgcaa actcagtgac attcgtcttt
    17941 ataaatccac ttattttatc taatggtcat gatatgtcat tcgtttcatt agcacttcta
    18001 gtaaatggaa tcgctggcgt tattggaaca tcattaggtg gtatattctc cgataaaatt
    18061 acaagtaagc gttggttaat gatttctgtt tctattttta tcgtcatgat gttacttatg
    18121 aatttaatct tacctggttc aggtctgttg ttagcaggac tatttatttg gaatatcatg
    18181 caatggagta ctaatccagc agtgcaaagc ggtgtgattc aacatgttga aggcgataca
    18241 agtcaagtaa tgagttggaa catgtctagt ttaaacgctg gtattggtgt tggcggcatt
    18301 attggaggct tggtcatgac acatgtttct gttcaagctg tcacgtatac gagtgccatc
    18361 attggcgcat taggattaat cgttgttttc acattgaaaa ataatcatta tgctaaaaca
    18421 tttaaatcat cataattctc atatgaaaag cacgcctgtt atcaaattca ggtgtgcttt
    18481 tttagatgcg ataacgttat tgatatgtgc gataatagcg acgttcatta tgatacatcg
    18541 gccaaggcat tttaccgctt ttagcaaaat tagctaaatc attttgcatt tgtcgactta
    18601 aaaatttaag gtgagtagtt gttggatatt gatgtgctgc taatatttgt aagtttccga
    18661 accaaaatac catatctaga atatggtaag cactgcgata gtgtgcgcta gaagtatcat
    18721 gccatgcaaa ttcagcaagc catagttgtg cgtttgaatc ttgtatattg agttgttgta
    18781 aaaattgctt catcggctgt ttgaaataac aatgtgtaat cgctaaagat tgttgcttcg
    18841 ccgtctgaac atcttcgtat tttaaaggaa tatcatttaa ttccataatg tcgataaagc
    18901 gttgcggcga taattttttc tgttcactag taatataaat atcgccttca tcttttgtat
    18961 aacatgcaaa aattggtttc gttgttggat aattattttg tatataacct gttttaatag
    19021 gcgcatatat taaatcaagc cctttagaag gtcctcgaga ttgttttagc ttctccatca
    19081 gcataagaat atcatctgtc gataatgatg taacatcatc tgtatctaaa taatcgcgca
    19141 tcaatttttg gaaatgttgt gctttattgc gtgcactctc aagggtgtct aatcgtagtg
    19201 cgccacttag caaaacgact ttatggaagt atggctcaat gtcaggtatt ttaagtaaag
    19261 tcaaaatgct catactgcct gcagactgac ccattaaagt aatgttatta gcgtcgccac
    19321 cgaaggattc aataaattga tgcacccatt ttatgacatt gatttgatct gaaagtccat
    19381 tattggaatg aaaatcttta ttaaaatatg accagtctaa atatcctaat gcgcctaaac
    19441 gataattgca tgtaataacg ataatgtcgt tattttgtac taaatgtgcc ggttgataga
    19501 gttcggctgt accatgacca ttttcaaaac taccaccata aaaataaatg atgacaggtt
    19561 tcttcgactg atcattatgt tgtttccaaa tatttaaata taaacagtct tcatgttcag
    19621 taaaagttgc agattgtgag gagaaaaaat cttctaattt gttgtctggt tgcggtggga
    19681 tgggttgtat ttcagttgca tcaattggct ctgaccattg tgtttttaac gtagaatgtt
    19741 taaagcgatt gtcatgaact ggtggttctg cataaggaat gcctaagaag atatctaaac
    19801 catcttgtgt aataccatga atttgaccac ctgtagtatt aatcttcata tttattaacc
    19861 tcctttatca tccccaattt aatcatacca cggtctatgg atgataggtg acaagtataa
    19921 caagacaatt ttgtggctga atcagacgag cttgatatct gttttaactc aaaccatttt
    19981 cagcattata tgatgttatt ctacagtgac atttcattac aatatcactc tcgctatctt
    20041 gttatgttag ttatttataa cccattttca aaataaaaaa ccaaccaaga tgttgaaatc
    20101 aatggttggt taatgtgtgt gttgaaatca tatatggcac tgcattttaa aatatttttt
    20161 gagatgactt tgattcatta tttagtgact cacgcatctt cgtattgatt cgtgttgcgc
    20221 gacggaaata gacgctttca aatgaactcg ttgtaccgat gtaatagaag ataagcgcca
    20281 caataataat aacgataaag tcaaatggat agtgaatcat atttaagcct ttaaattctt
    20341 tgcttcctat aaatgacagt attgatagca cgattaaata tacaataatc cataagctac
    20401 caccaatttg tttctttgta ttacgccaat tcatacgata ttcatagaag aagtagattg
    20461 gtaatccaag tataatgatt aaaataactt cagccgttgt tggccacatt gcccaatata
    20521 tagctaatga agctaataca aatgataatg gtgccattac ttttaaaatt tttgctctaa
    20581 atggacgagt cattgttggt cccatttttc ttaatgcaat cactgtcgtt gggccagtta
    20641 aataggctac taaagttgca gtagaaataa ctgctgctag cgtaccccaa tctctaaata
    20701 acgtaaccat aatcatacta atgatggcat taaagatgat tgctacacgt gggatatgat
    20761 atttttcatt catcttccca agaaatttag ggatatgtcc atttttttcc attgctcgta
    20821 aaactcgacc tgtaacggcg acaaatgaca cgccagtacc gaatggtgat acaaaagctt
    20881 caatatatag taaaattgcg agccaattaa ttcctaataa gatagctaaa tcagcaaatg
    20941 gtgaattgaa gttgatgcca ttccatccac tatgttgtaa cattgattga ggcatagaag
    21001 tgataaacgt actttgtaaa atgatgtaca acactgcact gattgacagt gaaatagcga
    21061 tgcctcttgc gatatttttt tcaggatttt taatttctga acccatatta ataattgtct
    21121 ggaatgaatt gaatgaaaaa ataatccctg atgctgttgt tgcagcaaaa atcggtgcac
    21181 ttccgtaagg cataaatgtg cttgctgaat ggccgtaatt tgaagtgtcg aatcctgata
    21241 acattaacat gatgatggtt aacatgggta cgcctaattt aaatacagaa attaaactcg
    21301 taaatgatgt taaaagtttt acggaccaat agtttaataa agaaaaaata acaatgatga
    21361 gatatacagc tagcaatccg tatgtgctaa tagagccatt ttccattaaa tgtttcattg
    21421 gtttcgccca atgccacggc caagaactca tatattgaac agctgacaca gcttcgatag
    21481 gtattattgt caccaaagac acccaattcg cccaagcagc aataaagcct aataatgagc
    21541 catgtgtata ctgggcatag ttactcatgc cacctgattg aggaaacatt gtgccgattt
    21601 caatgtagtt ataggcaatg gttccaataa ctaggaaccc aagaacccat gatatgattg
    21661 ctgctggtcc agcaattgat gatgcttccc acgcaccaaa tagccaacca gatcctatta
    21721 atgaacctaa ccctagtaat actaattggg atagattgat tttgtcgttg tgcaattgct
    21781 tgtctttatt cataaatact ccttcgttat tgccgaaaat tcaaaaaata gtatgcgaaa
    21841 aattatacgc ttattaatat aatagtcaaa tgcatttttt gtaagaatgc aaaagcattt
    21901 gtaaaattag ttttttataa aattaggata tataatacaa tgtgtattga aaaataaaat
    21961 ggaagtgatg actatgagtg agaaacaacc gaatgaacaa gttgaggcat ttttaaataa
    22021 agagagtcaa tggcaagaag aatataaata tttgagaacg ttaatcttta atgaaacaga
    22081 attagaggaa gcgtataaat ggatgcatcc ttgttacacg ttgaataata aaaatgtagt
    22141 acttatccat ggcttcaaaa attatgttgc actattattt cataaaggtg ccattttgga
    22201 ggataaatat catacactca ttcaacagac tgaaaaggtg caagcagctc gtcagttacg
    22261 atttgaaaat ctagaagaga ttcaagcacg tagagaagaa atcaaacact atattgctga
    22321 agcaattaaa gctgaaaaag ctggtaaaaa agttaaaatg aagaaaacag aggaatatgt
    22381 tattcctaaa gagttagaag ctaagttcga agaaatgcca cagctagagt cgtcatttta
    22441 taaattaacg ccaggcagac aacatcaata tatatatcat attggacaag ctaaacgcag
    22501 tgaaacaaga caaaagcgtg ttgaaaagta tattaaccaa atactagaag gtaaagggat
    22561 gcatgataag taattaatga gtaaaagcat accagttata caacaacata caatatgaca
    22621 tgaaacaacc aatggctcat gctgttggtt gttttttagg tgtgcctgtc atgggcaaca
    22681 ctttgacggt ggaattccgt tacaggcttg ggagtaggaa ctgttagcaa aaggcaaggg
    22741 tgtctacaat gaatgctgaa gatattaaaa tataaggatg actttgtgag tggcggatgg
    22801 acgtttatcc gcctgtaaca atggatgcgt gtgcattatt acaaaaattc gacttttgta
    22861 ataatatttc acattttcga cacttttttg atataaaaca accaattgag cgataataaa
    22921 ttcgcttttg aaaaatatga gttatctatt tagttgcaaa agataaaatg ataatgttta
    22981 ataacatcat atagagtatg ttagttttaa atgtcgaata tacgaatgtg caaacaaagt
    23041 aatcggtaga aattcaacat acatagcgcc gtttactgtt aagtattcac attacagatg
    23101 aaaaatataa aattctacat aatcaagacc atgatgtgta cttgtttaac ttatgactct
    23161 atttgtttaa caattgcgat gatggtcttt ttattttatg cgtatcattc gtcatatttt
    23221 ttatgaggaa ggagaaatga ttatgttaag tattaagcat ttaacgaaaa tttattctgg
    23281 taataaaaag gcagtagatg acatctcttt agatattcaa tctggggaat ttatcgcatt
    23341 tattggaacc agtggaagtg gaaaaacaac tgctttaaga atgatcaacc gtatgattga
    23401 agcgacagaa ggacaaattg aaattgatgg taaagacgtt cgtagtatga atcctgtcga
    23461 attacgtaga aatattggct atgttattca acaaattggc ttaatgcctc atatgacgat
    23521 taaagagaat attgtgttgg tacccaaatt gttgaaatgg actaaagaag aaaaggataa
    23581 acgtgcaaag gaattaatta aacttgtgga tttaccggag tcatttttag agcgttatcc
    23641 agcagaacta tcaggtgggc aacaacaacg tatcggtgtc gtaagagcac ttgcggccga
    23701 tcaagatatt attttaatgg atgaaccttt tggtgcattg gatcctatta cgagagatac
    23761 gttacaagat ttagttaaaa cgttacaacg aaaattaggt aagacgttta tctttgtaac
    23821 acatgatatg gatgaagcga ttaaattagc agacaaaatt tgtattatgt cagaaggtaa
    23881 ggtggtgcaa tttgatacac cagataatat tttaagacat cccgcaaatg attttgtacg
    23941 tgattttata ggacaaaata gactgattca agaccgtccc aatgacaaga ctgtagaagg
    24001 tgtaatgatt aaaccaatca cgatacaagc agaagcaaca ctgaatgacg ccgttcatat
    24061 tatgagacaa aaacgtgttg atactatttt tgtagtagat agggataacc atttactagg
    24121 attcttagac attgaagata taaatcaggg tatacgtgga cacaaaagtt tacgagatac
    24181 catgcaacaa catatttata ccgttcaaat tgattctaaa ttgcaagatt ctgtacgtac
    24241 gattttaaaa agaaacgtta ggaatgtacc tgtcgtagat gataagcaac gtttagtagg
    24301 actgattacg cgtgccaatg ttgttgatat tgtatatgac acaatttggg gcgatagtga
    24361 ggatacagtg caaacagaac atgtggggga agacactacg tcctcaaaag tgcatgagca
    24421 acacactact aatgtcaaag tacgtgacat aggagatgat aaatcatgat cgagttctta
    24481 catgaacatg gtggacagtt gatgtcgaaa acactggaac atttctatat ttctatagtg
    24541 gcattattac ttgccatcat tgttgcagta cctataggta ttttattatc aaaaacaaag
    24601 cgaactgcca atattatatt aactgtggca ggtgtcttac aaactattcc aacactagct
    24661 gtacttgcta ttatgatacc gatttttggt gttggtaaaa cgcctgcaat tgtagcgcta
    24721 tttatttatg tattattacc tattttaaat aacacggtac taggcgttca aaatattgat
    24781 agcaacatta aagaagctgg aaaaagtatg ggaatgacac aatttcaatt gatgaaggat
    24841 attgaattgc cgttagcctt accgcttata ctaggcggta ttcgcttgtc gtctgtgtat
    24901 gtaattagtt gggctacact tgcaagttat gtaggtgcgg gtggattagg tgatttcatt
    24961 ttcaatggtt taaatttata tgatccactg atgattgtaa ctgcaacggt actcgttact
    25021 gcactagcat taggtgttga tgccttatta tctttagttg aaaaatgggt agttcctaaa
    25081 gggttaaaag tatctggata attaggaggc taagataatg aagaaaatta aatatatagt
    25141 tgtcgtgctt gtcttatcgc ttgccgtatt atctggatgt agtttgcccg gactaggtag
    25201 taagagcacg aaaaatgatg tcaaaattac agcattatca acaagcgaat cgcaaattat
    25261 ttcacatatg ttacggttgt taatagagca tgatacacat ggcaagataa agccaacatt
    25321 agtaaataat ttagggtcaa gtacgattca acataatgcc ttaattaatg gagatgctaa
    25381 tatatcaggt gttagatata atggcacaga tttaacggga gttttaaagg aagcaccaat
    25441 caaagatcct aagaaagcaa tgatagcaac gcaacaaggc tttaaacaga aatttgatca
    25501 aacgtttttt gattcgtatg gttttgcgaa tacgtatgca ttcatggtaa cgaaggaaac
    25561 tgctaaaaaa tatcatttag agacagtttc agatttagca aagcatagta aagatttacg
    25621 tttaggtatg gatagttcat ggatgaatcg taaaggcgat ggctatgaag gatttaaaaa
    25681 agagtatggt ttcgactttg gtacagtgag accaatgcaa ataggtctag tctacgacgc
    25741 attaaactca gagaagttag acgttgcatt aggttattct acagatggtc gaattgccgc
    25801 gtatgatttg aaagtactta aagatgataa acaatttttc ccaccttatg ctgcgagtgc
    25861 tgttgcaaca aatgaattat tacgccaaca cccagaactt aaaacgacga ttaataagtt
    25921 gactggaaag atttcaactt cagagatgca acgcttgaat tatgaagcgg atggtaaagg
    25981 taaagaacct gctgtcgtcg cagaagaatt tttgaaaaaa caccactatt ttgataaaca
    26041 gaaaggtggt cataagtaat ggaaggtaat ttgttacagc aattattcaa ttattatgtt
    26101 acgaactttg gctatctatg ggatttattt ttcaaacact tattaatgtc tgtctatggt
    26161 gtactttttg cagctttaat tggtattcca ttgggaatct tgcttgcaag atacacaaaa
    26221 ctttctggat ttgtaattac aattgcaaat attatccaaa cagttccagt cattgcaatg
    26281 ttagctattt taatgttagt catgggctta ggctcagaaa cagtagtttt aacagtgttt
    26341 ttatatgcgt tacttccaat tataaaaaat acttatactg gtatagctag tgttgatgcg
    26401 aatattaagg atgctggcaa aggtatggga atgacgcgca atcaagtgct acgaatgatt
    26461 gaattaccgt tatctgtttc ggttattatc ggtggcattc gtattgcctt ggttgttgcg
    26521 ataggtgttg ttgccgttgg atcatttata ggagcaccta cccttggtga cattgtgatt
    26581 cgtggcacaa atgcgacgga tggcacaacg tttattttag caggtgcgat tccgattgct
    26641 atcattgcaa tcgtcattga tgtcctatta agatttttag aaaaacgatt agacccaacg
    26701 acacgacatc gtaaaaatca atctaatcat cgtccgcaaa gtattaatat gtaatagtag
    26761 aagatgttta taattttgcg attccgtttc atgatttata aaaaatgagg ctactcaagg
    26821 agctcaaata atctttgagt agccgtttta taggttgtgt ttgtatgcgt ttacactaaa
    26881 atagcaatta ttatcatgaa agtttttgga taaaaagcgt taattattgt aaaaatgcta
    26941 aaaaatgaga tgttttattt ataattttct gcaaatttat gatattgttt cttaatatat
    27001 catattaaaa atttgttttt cttaaacata ggaggcttat ctaattcatg gacacatcaa
    27061 aacaatttag aggtgacaac cgattgcttt tgggtatcgt tttaggggtt attacctttt
    27121 ggttattcgc gcagtcactt gttaatcttg ttgtcccatt acaatcaaca tatagtagtg
    27181 acgttggaac gataaatatc gctgttagct tatctgcctt atttgctggt ttgtttatcg
    27241 taggtgctgg tgatgttgct gataaatttg gtcgcgtcaa aattacttat gtaggattga
    27301 tattaaatgt tgtaggttca ttactcatca ttattacacc tttgccagca tttttaatta
    27361 taggtagaat aattcaaggt ttgtctgcag catgtattat gccatccaca cttgctatta
    27421 ttaacgaata ttatattggt acaagaagac aacgtgcctt aagctattgg tctattggtt
    27481 cttggggtgg tagtggtatt tgtacgttgt ttggtggctt aatggctaca tatataggtt
    27541 ggcgttcaat atttgttgtt tcaattctat taacattatt agcaatgtac ttaatcaaac
    27601 atgcacctga gactaaagca gaaccaatca aaggtatgaa agcagaagct aaaaagtttg
    27661 acgttatcgg gttagtcatt ttagtagtga caatgttaag tttaaatgta atcatcacac
    27721 agacgtctca ttttggttta gtttcaccgt taattctagg tttaattgta gtgtttatct
    27781 gttcattagt tggttttgta tattacgaaa ataaaataaa gcatccactt gttgattttt
    27841 caatttttaa aaatagaggg tacagtggtg caactatttc aaacttctta ttaaatggtg
    27901 tagcaggtgg tgcacttatc gtcattaaca cgtactatca acaacaatta ggatttaatt
    27961 cttcgcaaac gggttatatt tcattaacgt atttaatagc agtgttgtca atgattcgtg
    28021 taggtgaaaa gattttatct caacatggtc cgaagcgccc actattacta ggaagtggct
    28081 ttacagtgat tgggttaatc ttattgtcgt taacattttt accagaaatg tggtatatcg
    28141 tatctagtat agttggatat ttattgtttg gtactggttt aggattatat gctacaccat
    28201 caactgatac agcagttgct agtgcgccag atgataagtc gggtgttgct tcaggtgtgt
    28261 ataaaatggc gtcatcatta ggaaatgcat ttggagtagc agtatctggt acggtttata
    28321 ctgtgttagc agctaattta aatttgaact taggtggttt cacaggtatg atgtttaatg
    28381 ccttgctagc aattgttgca tttttagtca ttttactatt agttcctaaa aatcaaacga
    28441 atttgtaaaa ctgaaatgaa agcaagttat tatgtaggga ttttaaagga aattttgtga
    28501 aagtaagttt atcatacaca cttaatgttg cgtattgacg tttaatgtta agtgtgttct
    28561 tttatagacg ataaaagctg tgtgcatatt aagcgaatga atttcaaatt gacgctaata
    28621 tgcgaaagta gtattttaaa aatgaacaac aacgatgaag aggggtttat aggatgaaaa
    28681 ttgcaattgc tggatcgggt gcattaggta gtggctttgg tgccaaacta tttcaagcag
    28741 gatatgatgt cacgcttatt gacggatata catctcatgt tgaagcggtt aagcaacatg
    28801 gattaaatat aacgattaat ggagaggcat tcgagttaaa cattccgatg tatcatttta
    28861 atgatcaacc ggacgaaagc atttacgatg ttgtctttct atttccaaag tctatgcaat
    28921 taaaagaagt gatggaagct atgaagccac atattgataa tgaaacgatt gtcgtatgta
    28981 cgatgaatgg cttgaagcat gaagaagtca ttgcgcagta tgttgctcaa tcacaaattg
    29041 tcagaggtgt tacgacttgg acggcaggtc ttgaaagccc tggacacagt catttacttg
    29101 gtagtggacc agttgaaata ggtgaactag ttgatgaagg taaagaaaat gttataaaag
    29161 ttgctgattt acttaacgaa gcggaattga atggtgtcat tagtaaagat ttataccaat
    29221 cgatttggaa aaagatttgt gttaatggta cggcaaatgc attaagcaca gtgttggagt
    29281 gtaatatggc atcactgaat gaaagtagtt atgcgaagtg tttgatttat aaattaacgc
    29341 aagaaatagt gcatgtagcg acgattgata atgttcattt aaatgttgat gaagtatttg
    29401 aatatttagt tgatttaaat gaaaaagttg gtgcgcatta tccatccatg tatcaagatt
    29461 taattgttaa taatagaaaa actgaaattg attatattaa tggcgcagtt gcaacattag
    29521 gtaaacaacg tcatattgaa gcgccagtca atcgctttat tactgattta attcatacta
    29581 aagaaagtca gcgacatgca caggattaat tagtgttttt aggtgtgatt gcaaggaaat
    29641 gacaatcact tgatattacg gtcattatta aaataaatgt agtaaataaa gaacagcagt
    29701 aaggtacttt caaattgaaa tgatcttggt gctgtttttc ttgattggtc ttcgtcataa
    29761 ttcagatttg tcataggtct acgacatgct actagtattt gcaagacagt ttttacgacg
    29821 acactttgaa aaaatttgag gcaaatcatt tggaagtctc acgtgaattt tgtacactca
    29881 tcaagcaagt aattatatta aaagacaaat agagaaaagg tgtttataat gagtaaaatt
    29941 tttgtaactg gtgcaacggg ccttattggc attaaattag ttcaaagact aaaagaagag
    30001 gggcatgagg ttgctggttt tactacatct gagaatggtc aacaaaagct agctgctgtt
    30061 aatgtaaaag catatattgg tgatatatta aaagctgata ctattgatca agcgttagca
    30121 gattttaaac cagaaatcat tatcaaccaa attacggatt taaaaaatgt tgatatggca
    30181 gcaaatacga aagtacgtat tgaaggttct aaaaacttaa ttgatgcggc gaaaaagcat
    30241 gatgttaaga aagtaattgc ccaaagtatt gcctttatgt atgaacctgg cgaaggatta
    30301 gcaaatgagg aaacttcact tgattttaac tcaactggtg atagaaaagt aacggttgat
    30361 ggtgtggttg gtctagaaga agaaacggct cgtatggatg aatacgttgt tttacgtttt
    30421 ggttggttat atggcccagg tacttggtac ggaaaagatg gcatgattta taatcaattt
    30481 atggatggtc aagtgacact ttcagatggc gtaacatcat ttgtgcatct tgatgatgca
    30541 gttgaaacat ctattcaagc tattcatttt gaaaatggta tctataatgt agcagatgat
    30601 gcacctgtta aaggttctga atttgcagaa tggtataaag aacaacttgg tgttgaacca
    30661 aatattgata ttcaacctgc gcaaccattt gaacgtggcg taagcaatga gaagtttaaa
    30721 gcgcaaggtg gtactctgat ttataaaact tggaaagatg gcatgaatcc aattaaataa
    30781 tcggtttctg catatagcat gttaataatg aagacttgtt caaatcgcta atcctcatac
    30841 ataaatatgt atgtttcgga ttgatgtgcg attgaacaag tcttttttat ggtttcttaa
    30901 ttgtcgttgt atgtagtact aacagaattg taattaacat actgaataat gcaataaatg
    30961 ttgtagttaa cgttaattta atttctattg gcagccaagt cagtacaaaa gaccagttat
    31021 tgctaccgag aatgagatat ggtaatgcat ataatatgag tgctatagcg atacataaac
    31081 ataaagttag ccaacttaaa aatgtggtaa gttttgattt gcgcaggatg attttcccgt
    31141 gacgcatttg ccaagcacga taagctgaga atataaatgc taataaaata agtgttgtca
    31201 tcaaaacggt aacaatatta aattgatctt tatattgatt gagcatagac gcaactgtcg
    31261 aatatcgctt gtgatttaca atttgtgtgt taagatgctc aactaatttg ggtacatatt
    31321 ccgaatttag atttgcaagc acaacaattc cataattttg ttttggattt agtaaaataa
    31381 atgatgaaaa gttatcgagc gttcctgaat gaaacactaa atgttcatca ttattggtaa
    31441 accagccgga agcatatgca ttggcattag gttcaccaat tgttgatgat aaatttttat
    31501 gtgattgttg aactaatgat ttgtatttat caggtggatt aagttggaat tttatccaat
    31561 gttccaaatc ttcagttgat gtcatcatat atgctgatgg tgtatcccaa aggttaaatt
    31621 caggtttaga gacgacaggt gtcgaacctt gtaattcgta gccaatagca tcattttttg
    31681 atttattatt ggtttgtttg aatgatgtat gtgtcatatg caaaggcttg agccatgaat
    31741 ttgtaatata ttttgtatag gattgcttcg taacgttttg gataattaaa cctaataaat
    31801 catagttcat atttgagtat tcaaattctt ctccgggctt atgatgtaat tcatcaccca
    31861 taattgcacg ggttacatca tttaaacgat tatttttatt tgttacggcg tcttcgcttg
    31921 taatatcact aggtatacca cttgtttgag ccaaaagttg cttaatcgta atagtttcat
    31981 tttgaccatt atagttcatt ttaaaatgag gcacatgttt ggatacgtca tcatttaagt
    32041 ttaatcgacc ttcttgagct aattttaaaa ttgcaagacc tgtgaaagct ttcgtattag
    32101 aagcaatttc atactttgtt gtgggagaag ctttgacttt tttatcaaca tcttgataac
    32161 cataaccttt atttaaaaaa actttgccat tttttactat taaaattgat gctccaggaa
    32221 tatgtccctt ttgtaaatca tgttcgataa ttgtatctat ttgttgttgc gaatcattgg
    32281 ttaaccgtgt cttcgtattg ctatttaatg ttatatatat agcaattgaa atggcgacta
    32341 aaatgataat agaaatggat agaaaataca gttttttagt agtcataaac gttccttttt
    32401 atatgttaaa tttacttaag tgcctaatga gatttatttt attataccag ttctattgaa
    32461 taaattaatt aaagaggtat atttaccatt atttttgaca ccttatatgt taataggtgt
    32521 ataattacat aaaaaatatt aattaactct ctatactttt tggaaagata ggcgtatagt
    32581 gatatccatc aaaaagtata aaaaggaata ggagattatg ttcaatcaat ttaaaagact
    32641 tattataggg caacctaaaa aaaatagaga actaaaagac gaaaagatat cgaagtttaa
    32701 agggttagcg attctttcgt cggatgcatt atcttcagta gcttacggtc ccgaacaaat
    32761 actgattaca ttatcagtag tgggtgcagt tgcaacttgg tatactttac cgattgcagg
    32821 tgctgttctg atcttattag ctgctttaat tatgtcttat aggcaaatta tttatgcata
    32881 tcctaaaggt ggcggcgcgt atatggtgtc caagaccaat ttaggagaaa aatggggatt
    32941 gcttgcagga ggatcattat tagtcgatta catactgact gtcgctgtta gtatatcatc
    33001 tggtgctgat gcatttgttg ctgcattccc aagtttatat gggcataaag tactgattgc
    33061 atgtttactt gtgcttttta ttttaatatt aaatttacgt ggtttaactg aatctgccac
    33121 cgtattatcc tatccagtat atcttttcat tatagggtta gtgatattaa tatttatagg
    33181 tactttccgt gtggcgacag gagatattca accacatatg catgcatcag taggaactgc
    33241 ggttcctgga gtaacattat tcttgctatt aaaagcattt tcatcagggg cttcatcatt
    33301 aacaggtgtt gaagctattt caaatgcggt aaccaatttt agagaaccaa gtgctaataa
    33361 tgctgtaaaa acgttaattg ctatgggttc aattttagcg tttttattag ttggtatcgt
    33421 tggtttagca tatgtttatg gaattatgcc acaaacggaa acgacagttt tatcacaatt
    33481 agctatgcaa atatttggtg ataatgctgc cttctatttt gtacaggcaa cgaccgtgat
    33541 gattttggta ttagcagcca acactggatt tacagctttt ccgatgttag cggcttcaat
    33601 gtctaaagat aaatatatgc ctagaatgtt tactgttaga ggtgatcgct taggctactc
    33661 taactcgatt attattttag gtgtattagc cattatttta attattgtat ttgatggtat
    33721 gactgaagat ttaattccat tgtatgcagt tggtgtattc attccattta cactagcaca
    33781 attcggtatg gttattaaat ggattcatga acgtcctaag aattggttaa gtaagttgtc
    33841 tgtgaactta cttggtggta tcgtgacatt tattgttttt atgattttac tcataactaa
    33901 atttagccaa gtttggccga tacttatttt cttgccgttc gtggtgattt tcttcttgaa
    33961 aattaacaaa cattatcgag atatcgcaga acaattacgt tctgatattg atgttcataa
    34021 tgtagaagta gtagatcgta atttagcaat tgttccgata acaagcatta caactgcagt
    34081 tgataagtct atttattacg cacaaatgct tgcgaataat gatgtcattg cagtacatgt
    34141 atcatttgga gatgaagatg aaaaagcatt ccaagagaaa tggaaacgtc atttcccaga
    34201 tgtcagatta gttattttac attcggaata tcgcagtatt atacgaccaa tttcacgttt
    34261 tatcgataag attaatcgta aagccaacga tcaaaactat atgattacag tagtgatacc
    34321 agaatttatt actaagaaac gttggcataa tttattacac aatcaaacta gtttgcgtat
    34381 gaaattgtat ttaatttatc aaaagaatgt taacgtatgt acaattccat ttaaactaaa
    34441 aaaatagatt ctataatcaa atcggattga tgaaaggtta tttacttttc atcaatccga
    34501 ttttttgatg ttgactataa aaaccccgta catatgagat gataatgact ttgtctcata
    34561 tgtacggggt gttgttatgt cttgcttaat gtttggactg taaagacaag acaatttcgt
    34621 tgtaattgat attttctcga atttgagctg caacgatatt gttgatttta tttttacgaa
    34681 ttaattcatc taaaagatga cgttgtagat acaatgcttc caatttgtgg ttatggaaca
    34741 tagcttgcgg tatgatttga ttgctgtttt cttgacgttc ttttcgacgt tgtgcacgcc
    34801 actttaatga gcgactcata tcatagtaat gattaatgac taagctaacc tcaagtacat
    34861 ttgtactatt ttgttcttct tttaagcgta aaataatatt gtgattcaca atacgcataa
    34921 ttttttgaac atctttgaat gaattttcaa attcttgctt cttagattct ttgtttaagg
    34981 ctttgtagtg cttcatcgct ttaagtctaa gccataacat cttgaagaag tttgcaaatt
    35041 tttcaacaaa tgatgcagta cggtgtgtct ctgttaattc gacaatattg cggtaattat
    35101 taatatcctg atatgttgcc ttacctttat caattaaacg ctcaagtgta gatgtttcga
    35161 ttacttttgc aatatcttct aaacgtttga gttcttttgt attttgatta tcaggttctg
    35221 aatttaataa aaacaacaat tctccaaagt attggtttaa aactgggcga taatttgtat
    35281 cgtttttgtc ttttttactt tcgtttttaa aatgttcgat aactttttgg acaatgaaaa
    35341 ttttggcaga ttgataagtc atacctttaa aagtagtatc ttcggcagat ggtgtaatta
    35401 aaggtaaaac aatttgcgct aagattaaac taattaatac catgaaagat gcaataaaca
    35461 ataaatcatt acggtattcg aatgcttgtc cttttgtaat gataaatggt aatgtaagtg
    35521 ccattgaaag tgaaattgta ccgtgaatac cacacatggt cataataaat gcgtaacgca
    35581 cacgagaagg tggtgtttca tgtgaatcat gttcctcgtc tagataagat tgtatatttt
    35641 tcgggaaata aaaatctttg taccagaaat aaacccaaac aaatcgacat gcataaattg
    35701 caatagcgat taatatagtg atcactatta aaaatgaaaa gttgtcaggc tctgtttgga
    35761 aaatatctat gactacagta ggaatcataa aacctaatac aacaaataca aaaccattta
    35821 aggcataact aaatgtgttc caaatttgat ggtaattcat ttgtagttcc gtttgtgctc
    35881 taattaaacg gtcgcgttct aaaccatgga ttagacctgc gattacaact gcaatgatac
    35941 ctgaagcatg aacttcttct gctaaaaagt atacgacaaa aggagttaat aattgaataa
    36001 aagttaaggt attgttatct tttaaacctt tattagcggt taagtctata cgtattctaa
    36061 cgacaacgaa tccaataatt gcaccaataa gtacacctag tattgttgaa atgataaatt
    36121 gttcaacagc ttggaataat gaaaaggtac cagttactaa tgcagtaaca gcaattttaa
    36181 atgaaatgat acctgctgca tcattgagta aagattcacc ttctaaaatc gtcatagaac
    36241 ctttaggtaa taattttccg cgtgtaatag cagatactgc tactgcatca gtaggacata
    36301 aaattgctgc tattgcaaaa gcggctggca ttggtaaggc aggccaaatc caatgtataa
    36361 aatagccaac accgactaca gttgcaaaca ctagtgccat tgacattaat agtataggtt
    36421 tacgatattc taataatttt gttcgagaga cgtgggtacc ttccacaaaa agtagtggcg
    36481 cgataacggc aaacataaat acttcagaat tgaattggaa atcaacttgt attggaataa
    36541 tgaaaataac gacacctaat gcaatttgaa taaaggcagt aggaatttgt gggaatcgat
    36601 tattgataac cgaactaata atcacagcaa aaataaaaat taaaaatgct tctaatagtg
    36661 ccatacaata ctcctcaaaa ttttaatagt taatatttta tcacttttaa ggcataatga
    36721 catagttata ttgataaaat gaagttaatt tcaaaaaaac tctagtatcg gttgaactga
    36781 tactagagcg agatgtttaa attattgatt gtcatatctg aaatgaccgc tgtcattttg
    36841 tcgttgttca tatgcgagct tttcagcatt cgttttgtat tttttataaa agaaaaataa
    36901 aaatataaac cagaatggcg aaatataaat agctgctctt gtttcgtcac taaagaataa
    36961 taaaatgaat acaaagaaga agaacgctag aataatgtaa gcaataggct taccaccaat
    37021 taacttaaat ttactgtttt tatgtgcctc aggatgcttt ttcaaataca ttagataagc
    37081 gacaataatc attgcccaaa caactaaaaa caacacagtt gataacgttg taacgtatat
    37141 gaatagttga attgcattag ggaaaatgta gtttaacaag gctgctataa gtaatgcaat
    37201 tgatgaaact aacattgata aataaggcac gccatgacta ttcgttttat ttaaaacttt
    37261 aggacctaac ccttgttgtg acagtccgaa taagatgcga ctattcgaat agataccact
    37321 atttgtagca gaggccgcgg cagttagcac gacaaagtta acgacacctg ctgcaaatgg
    37381 cacgccgatt aatgtaaaga gtttaacgaa agggctacta tttggatcga tatcattcca
    37441 aggtatgact gacataatta ctaatagacc accgatatag aataataaaa tacggatagg
    37501 tacattatta attgctttcg gtaaggtttt ttcaggatct ttcgtttcac cagcagttac
    37561 acctataagt tcaataccaa tgaatgaata tacagcaatt tggaatgaca ttaagaaacc
    37621 aaatgttcct ccagggaaca tgccgccgtg actgattaag tttgtgaatg atgcatgtcc
    37681 ataatgtgtt ttaaatgaga aaaagattaa tacaagacca acgataacca tcgcaataat
    37741 tgtgacaact ttaataatag agaaccaaaa ctccagttca ccaaataatc tggcacctaa
    37801 taagttgaag ctaatcaaga ttaaaacaat aaatagcacg gtaatccaat ttgggacttg
    37861 tggataccaa aatgcaaagt attgtcccat cgcagttagg tctgacatac ttgatacaat
    37921 ccaacataac cagtaagtcc agccaatgac aaagccacca aaagggccta aatattcatt
    37981 tgcaatatca acaaatgaat taaatcttgt attgctcaac aacaattcgc ctaatgcgcg
    38041 cataaaagcg aatagtacaa ccccaataat catgtatgtg aataacagtg atggaccagt
    38101 taaggatatt gtttgacctg atcctaggaa taacccagtt ccaattgcgc ccccaattgc
    38161 tatcaattgg atgtgtctgt tattcaactc tctatgcaat tttcttgcca taattttcct
    38221 cctcaggata aagcaaataa aaaagttgtg tgtaagcgtt ttcttaatta taaatactaa
    38281 agaaatccat ttaataaagc aattattttt ttaaatataa gaaaaaacat ttgatagcta
    38341 agggtatgat aactaaaact tatcgatttt tttgaaaaga tataagtttt tgtgcaaaaa
    38401 tataaaataa tcatctaaat gaagcagtta actatatata ttagataagc tatggcataa
    38461 taaataacat tttaatttga gtaaagaagg tatgggtatg aagataggga caatcgccga
    38521 tttacatatt gatcgtcata ataaaaaaac atccgaagat tacttagagg cattggttga
    38581 aatagttaag tataaaaatt tggatatatt gttgattgcg ggggatatct caaatcatta
    38641 tcaattgacg caccaattta taacgcaatt gacaaaacaa ttagatatac ctgtgaaatt
    38701 tgttcctgga aaccatgatt tatgggaagt tgaaagcatg actacgcaag acatttggaa
    38761 taattataag agtatgtcac agtgcttggt agcaaaacca tttatagtaa atgaagaatg
    38821 ggcaataata gggcatacag gttggtatga ttatagcttt gcagcacaac gattttcatt
    38881 agatgagtta caaaaaggaa aacattatgg tgcgacttgg caagataaag aacgaatatc
    38941 ttggggcaca tcagatcaaa atttatctaa aatagcggct gaacaagtga agaaagatct
    39001 attagaagta ggaaatagac gagtgatttt agtcacacat gttgtgacgc accctgattt
    39061 cattgttcct atgccgcatc gtatattcga tttttataat gcatttattg ggacaagtga
    39121 tttcaatcct ttgtatgcga tgttcgatat accatatagt attatgggcc atgttcattt
    39181 tcggaaaagt gtgatggatg atggcagatg ttatctctgt ccgtgtctag gctatccaag
    39241 acaatggcgt tcagaagata tttaccagga aataaatgag acgatacaaa taatagaaat
    39301 ttaaaatgcg caaacctgac ccagtttgcg cattttatgt tttacacacg cgagtaatgt
    39361 gtttacttac gtgtgtttat tttgttgttg attttcaatt gtatatgaat gtggttgcac
    39421 ataaatgcac tttcttcctg gtgaattaaa gctgtattcc attttctctt tacgaatttt
    39481 aataatttgt ttgcgatttg gaatgatggc aggtaaaact aggccacgac gaatatgact
    39541 ccaaagtgga tcgatataat agtaatagta atcatcgtac ccaatgagta acgttacgtg
    39601 aatatttgaa acttgtttcg ctggcttgtt atcaaagcgg aaaacacgta gtaatggttt
    39661 agaaccaaga ttagtatggt atattaacac aggttgacct tgatcgataa tacctttaag
    39721 atcttctaag gatttaccag tgccgtctac gatattagga ttgtattttt gtaaaaatgg
    39781 tacatatgct tctggaaata tcgtttgatg ataattgcca agcttaatga ataagtgatg
    39841 tccaacataa cctttatgtg gattgttcgg atgtgtcggc caatgtctca taatttctgt
    39901 agcagggata tgttggttat tgtattgcaa catcatggct gcggaaacac cttcacaccc
    39961 catgaccata gggataggaa atagctgact gataggttta actggtaata tttttcggtt
    40021 cataatatag tcctcgcatt gattcaataa atatttaata taattatata gcgtcaatgc
    40081 aaaatgtcct aaacatatgt tttacataag tgaataaaat taatggagtg ataaaatgga
    40141 atatcaatta caacaacttg cgtcgttaac gttagtaggt attaaagaaa cgtatgaaaa
    40201 tggacgacag gctcagcaac atatagcagg gttttggcaa agatgttatc aagagggagt
    40261 aattgcggat ttacagttaa aaaataatgg tgatttagcc gggatacttg gcttatgtat
    40321 acctgaatta gacggtaaga tgtcatatat gattgcagtt accggagata atagtgctga
    40381 tattgaaaaa tatgatgtca taacattagc aagttcaaag tatatggtat ttgaagcaca
    40441 gggtgcagta cctaaagcag ttcaacaaaa aatggaagag gttcatcact acatacatca
    40501 atatcaagca gatacggtaa aatcagcacc attttttgag ttgtatcagg atggtgatac
    40561 tacaagtgaa aaatatatta cagaaatttg gatgcctgtt aaagggtgat tgaatagaaa
    40621 tgtacttgat atgaaggcgt aattttaatt gaagattgga tctcataatt cataaagaag
    40681 aaccccgaca aagatagata tctttgctag ggttctaatt atgttgaagt gttatgcgac
    40741 atgttgcttt ttaatcattt taaagtaaat gatttgtaga ctgactaata gaatggctgt
    40801 aatgaagata ataatcatat atggtgatgt attaaattcg ccttttaagc caactaatgg
    40861 tgcaacagcg ccacctaaga taaattggaa taagccaagt aaacttgatg cgttaccact
    40921 gccacctgtt cgttcttcca tagccattgt gaaaccaagc ggtccaattg acgtcacagg
    40981 acacacattt aagaagaatg cgatgagtaa tacccaaagt ggtaaatgga atgtaagtgt
    41041 caggataatt aaagcaacac ctaccacttg tataatagtt aaaatgatta ataatatgtg
    41101 gcgatgtaat ttttctacta ataaagcaac gacttgactg acaatgatta aacctacacc
    41161 gttaacagca aacatcatac taaattgttg gggtgtcata ttatacaatt tttgagtaat
    41221 aaatggcgat gcagatgaat aactaaatag cattacataa gttaaacctt gtaatagcat
    41281 tggaataata aatgctggtt ttttcaaaag acttccaaag tctttaatga cgctactaaa
    41341 attcacctgc tttaattctt ctttaggtgt tttaggtaat tgagaagcga cgccaattaa
    41401 aatgatgagt gccacaattg ttaaaattgt aaaaatagaa cgccatgttg ctacggataa
    41461 agctaatcca ccggctaatg gtgcaagaat agtgatgatg ccattaacta ccattaaaga
    41521 tgctaaaaat ttagcgagtg cgttgccact aaatttatca ccagcagagg ctttggcaat
    41581 aacgatgacg ccaccacctg tcaaaccttg aataaatcgt aaagttaaga ataatggcaa
    41641 ttgatcaaca aacatagaca aacctgatgc caaaatgaaa atgattaaaa tggatacagc
    41701 aatgcgtttg cgaccaaaag catcggataa aggtccgaag ataaattgac caagtgcaag
    41761 accaatcatt gtgaatgata aggttaattg aatttctgat gttgtcgaac caaaatcacc
    41821 ttgcacttta ggtaatgatg gtacgtacat atctatagtt agagggccaa aagctgtcat
    41881 gacacctaac gtaattaaca ataaaaatga aaattgcttc gttttggaac tcgaagattg
    41941 cataaacaga ctccttaatt tatataatta tgtaaattat atacgtttta tgtgcatttg
    42001 tatgtagtta gaatataagt ttatataaaa agtcctatac tcgcaatgta tcatcattaa
    42061 taacgtagtg atacagataa agtataggac ttaattaatt tatttatata cttagtgaaa
    42121 ggtgaaataa ttaattacca ccactattgt cgtctttaaa tagactttct aatttagatt
    42181 taatactgtc gaattttgta ttaatatcat ttaacatgct ttcaatttta ccttggcttg
    42241 cattgttttt aagtgcatta tttgcctctg tcaatttgtt gatttggtct tgataaaatt
    42301 gagttagctt agaatcagct ttgttttgtt cttgctgctt caagttgtcg atttgatttt
    42361 gtaaattttt aataatgtca tcagtttttt ggttgttttt aatattttct ttagcattat
    42421 cgacttgctg gttaatttga ccgtcttgat tttcaacttg ttttttataa ttttgagaga
    42481 ctttagggtc attactaact ttatcgctat cattgttaag taaggcgcgt gtgacaaaaa
    42541 tagcgacaat aattaagaga atggcaataa tgatatataa ccattttttt gaacggcggg
    42601 atttctcgtt ttcttcttca agaatttgtt ctcttcgata atctctaccg ttatagtatc
    42661 gttccgggtg ttcttcagga tcgcctttag catattgttg tctatacgat gcgtcttcag
    42721 attgacgacg atgattttgg ttttgattgt cgtattgata tgaatcacgg tatttatcag
    42781 ttcttttcat gggtttcacc tcattcaagt tttaactgca aataataaag ctgatgattt
    42841 actttgacat tattgtaatt tcagaatacg agcaatgtca gttgcagtgt ttaataaatt
    42901 ttgttcgcta ttttgtagga catcttctaa agtgctaggt gaagagatta tagaataggc
    42961 actatcaata ccgaaatcgt aaacatgttg ataattttcg cctagactgc cacaaatcgc
    43021 gatgacagga atatgatatt gttttgcagc taacgcaacg cctacgggtg ttttaccaaa
    43081 gatggtctga taatccatgc gtccttctcc agtaataatg aggtctgcat ctttaattct
    43141 ttgatgaaaa tttgtaatgt caaagacgat atcaatacct tttgttaatg ttgtgtcgaa
    43201 gaaagctaat aaagcagcac ccataccacc agctgcacca gaacctggta cttgattaac
    43261 ggattttcct gtacatattt ttatcttatc atgataatgc gacattgcga aatccaactt
    43321 tggtatcatc tttgcatcag cgcctttttg aggaccataa atataggtag caccattttc
    43381 acccaataaa ggatttgaaa catcacaggc cactttaaag gtcacctctt ttaatcgcga
    43441 atctagattg gttatatcga tttgtgcaat gtgagcaaga ttagcaccat tcatttgtaa
    43501 taagtccccg tttacatcag taaactttac gcctagtgca cttagcatac ctgtaccacc
    43561 gtcatttgtt gcactgccac caatccctaa aataatggtc ttagcaccat gatttaatgc
    43621 atctttaatt agttcaccgg taccatatga tgatgtgtat aaaggattac gttcctcttt
    43681 ttctaataaa tccaaacctg acgctgccgc catttcaata attgcaattt gttgttcgtc
    43741 tgcgcgtgca taacatgctt caataggtcg cattaaaggg tcattaacga tgactgtata
    43801 cttagtggca cctgttgcat gcattaaagc atctgtggta ccttcaccac catcagccat
    43861 cggaatgata tcataatgaa gggtattccc ataaacatta gtaaatgcct gttttataat
    43921 attgccaact tgctgtgcgg tcatactttc cttaaatgag tcaggagcaa tgacaatttt
    43981 ttgtaattgc atagtagaca tcctctcatt actgttgtcg ctcttattgt atataaaatc
    44041 acttgcaaac taaagtaatg tctatagcgc taatgagtcg cacgataaat cagttaaatt
    44101 ataatggcgt ataaggatta ggggaaggga ctaaatttgc tgtgaaaggg tataaagatt
    44161 gaaataagca tatttgaaga gaagcggatt ggctcgcata ttcgagtatg ttggagaacg
    44221 cagttataca tatattttga tgttgaggag ttacacatat gaaactaaca caaacgcatg
    44281 ctgaaatttt aaaatttatc attgttggcg gcattaatac gttaaactat tatgttgtct
    44341 atttattgct gttaaaatta ttacacattg aatatatgat tagtcatatt accggattta
    44401 tagtcgcttt tgtgatttcg tattatttga attgttattt tgtttacagg gtaaaaccta
    44461 cttggagaaa attcattagt tttccaatta cgcagcttgt caacgtaagc ttacaaacag
    44521 ttttattata tgtttttgta tcctggctaa atttgccagc ggaaattgcg ccatttgctg
    44581 gtttaattat tacgataccc atcacattta tattatctaa atggatttta aaagatagta
    44641 attaaagtag cgtaaaccac taaaaaacgc tttactcgaa ctttaatatt gcgtgaaata
    44701 taataaataa atgatataat ataatggtcg cctaacatct cgggcgttac tttaacgcag
    44761 agaggaggtg tatgacggat gttcaattta ttaattgaca tcatgacttc agctttaagc
    44821 ggctgtcttg ttgcgttttt tgcacattgg ttacgaacgc gcaacaataa aaaaggtgac
    44881 aaataaccaa cacctaaata aatcccctca ctatggcagt agtgagggga tttggtgtat
    44941 ttagatgttc aatttattat gtaactaatt atagcattga tgaacaaggt gtgcaatgta
    45001 tttttgcaat attacaataa atgaatttaa ttacattaga attaagtatt tcgaaaaatg
    45061 atttaatagt tgagtcatag gatcattaga taaagcgaat aatcaattat agggacattg
    45121 ggatacaaat caatgtacta gactctacaa ggctatactc agggacccaa catagagaaa
    45181 ttggatccac agtttctaca gacaatgcaa gttggcgggg ccccaacaca gtagctggcg
    45241 gaaagtcagc ttacaataat gtgcaagttg gcggagcccc aacatagagt tttactcatg
    45301 tattcctatt tttaagtaca tattagctgt ggctaaggat aaagaatcat tacataataa
    45361 atcattagtg gctctttatc atttctgccc cataacattg tgaattttta aaagaaatgt
    45421 gtcacaaatc cacgcgacat tgataatcgt tatcaattaa tatatgatgt acttgtaata
    45481 agtattaatt tttaacaaat aacaaattta tattatgtat atcatccagt gaaataaata
    45541 atataagcgg cttaatttag attattaatt gaaaggagtt acatatacga tgaacaatca
    45601 gacaaattgg ataaagatac tcagcggatt tgcttctgat agtaaatgga aaattatgtt
    45661 atcaattttg ttgtctatca tcagtgtctt ttctggatta gttccatatt gggcagtctt
    45721 taaaattatt ttaatgatga ttaacaatgc atatacgatt aattcgatta tggtttatat
    45781 ctttattgct ttaatcgctt atatttcaca agtgtgttgt tttggagcgt caacgatgct
    45841 atcacatatt acggcatatg aaattttatc tgaaattcgt aaaaaattag ctcaaaaatt
    45901 aatgcgtctc cctttaggcg tagtggaatc taagaaaata ggtgaattaa aaaatatatt
    45961 tgtcgataag gttgaaacaa tagaattacc tttagcacat atgattcctg aagttatagg
    46021 aaacttactt ttgtcagctg ctatcttttt atacataatg ctcattgatt ggcgtatggc
    46081 tagtgcctta ttagtaacga taccaatttc aattttcgct tttaaaaaag ttatgtctgg
    46141 atttaatgag acatatgctg aacaaatgaa atcgaataat tatatgaata gcgcgattgt
    46201 agagtttatc gaaggcattg aagtgattaa aacatttaat caatctcaaa gttcatataa
    46261 aaaatataaa gatgcagtag ataattataa gattcacact ttgaattggt ttaaaaatac
    46321 atggggatat atgaatttag gttcaagtgt attaccttca acatttttag ggattttacc
    46381 ggtcggcatg tatttgatat ctatcaacca attaaactat gcggaatttt tcctatgcat
    46441 cgtattatct ttaggtgtag tagcaccgat taagaatttt actaattatg taaatcattt
    46501 aaaatcaatc caatatgcgt taactgaagt aaatcaatta ttaatcttag aagagttagc
    46561 attgtcatca cgatttatgg aacctcaaca ttatgaaatt gcttttaata atgttggatt
    46621 ttcatataac aaagataagg acgatctggt ctttaagcat ttatcattta cagtaccaga
    46681 aaataatttc acagctatcg tcggggcatc tggtagcggt aagtcaacca ttgccaagct
    46741 tatatcacga tactgggatg tgacttcagg tgaaattacg attggcggta taaatataaa
    46801 agatattgaa tcgaaacaac ttaacgattt agttggtttt gttggacaag ataacttttt
    46861 attaaatctt acttttaaag aaaatattaa acttggcaat ccagaagcta cggatgaggc
    46921 agttgaaaaa gctgccaagt tagcacaatg tcatgaattt attgaaaagt tgccagatgg
    46981 atatgatacg aatgttggta cagtgggaga taaattgtct ggtggtgaaa aacaacgagt
    47041 cactattgca agaatgatat taaaagatgc gcctatcatt gtattagatg aagcgactgc
    47101 ttacgttgat ccagacaacg aacaaaaaat tcaagaagca ttaaatgtgt tgacgcagga
    47161 taaaacattg attgttattg cacatcggtt atctacgatt caacatgcag atcaaattat
    47221 tgttttaggt aaacaacaaa ttttagaaaa agggtcacat cacttattgc taaaattaaa
    47281 cggtaattat aagaaaatgt gggatacgca catgcataca aaagattggg gaatcaatac
    47341 tggacataat taaactaatg agaaaaggag gtcaaatatg tttcaaatta catttaaaat
    47401 tttaaactgg atgagaccat ataaagcaag aatgatttta ggatttagca tgtcattttt
    47461 aaatgctatt tttattgcgt tacctatctt tttagctgca aaaatattta ataatgtgct
    47521 atctcataaa cctatttata tgaaagatat attgaatgtt gtaattatca tggttttatt
    47581 agtgattgga cgatttatta cagcatattt caaaagcaaa agccatgaga gcatcgctta
    47641 tgaaatgagt gcgaaagaac gtttagatat aggggataaa ttgaaaaatg taaggttagg
    47701 ttattttaat tcgcatcatt caaatgagtt aacaacaata gtaacaacag atttaacctt
    47761 tttagaaaac tttgctatga aaatggtgga cgttgttgtt aatggataca tattaattat
    47821 agtactcata ttgtctctac ttgtagtttc gtggcaagta tcattattag catgcattgg
    47881 cgtattacta tcattttttg cgattcaatt attagaaaga aagagtcgac aaaatgcgcc
    47941 agcgtatcat aatgtacaaa accaattagt ggaaaaggta ttggaggtta ttcgtggtat
    48001 tcaagtaata aaatcattcg cgaaagaaaa tacgagtctt aaaagtttta accaatcagt
    48061 caatgaaagt aaacgtataa atacaaaaat agaaatgcaa tatatcccat tcaatttatt
    48121 gcatttactt agtttaaaag ttgtttcaat aatgattgta ttagttgcat gcttattata
    48181 tatgaatcat agtattgatt tacctaccct tattatgatt tcaatttttt catttgtgat
    48241 atttgatagt gttgaaaata ttaatagtgc tgcacacgta cttgaaatga tagatatgac
    48301 gatagatgat attgaaaaga taaaaaatgc tccagaactg gatgagaatg gaaaaaattt
    48361 gacgattaaa aatgaaaata tcgcttttca aaacgtgaat ttttcatatg atgataaaca
    48421 agtgataaag aatgtgaatt ttgagatacc tacacaaaca tcaacagcaa taattggacc
    48481 ttcaggaagt gggaaatcta cattatgtca cttactcttg cgcttttatg atatcgatga
    48541 tggaattatt cgcatcgatg gtgttgatat taaagatatg acattaagta cgttaatgtc
    48601 gaaaattagt gcagtatttc aaaaggtgta tttatttaat gatacgattg aaaataacat
    48661 attgtttggc aatccaggtg caacgaaaga agaaattatt cgtgcagcga agcaagcatg
    48721 ttgccacgac ttcatcatgt cattacctga gggatatcaa acaatgctaa atgaaaaagg
    48781 tagtaattta tctggcggag aaaagcaaag gatttctatt gctagggcga tattaaaaga
    48841 tgcaccaata attattttag atgaagcaac ttcaagtatt gaccctgaaa atgaacagct
    48901 gattcaaacg gcaattaatg aattaagtaa aggcaaaaca gtaattacaa ttgcacataa
    48961 acttgaaact attaaaaatg cagatcagat tatagtgctc aatgaaggtg aaataattca
    49021 aaaaggtagt catgatgaat taattcgaaa accaggaatg tatcaagact ttataagaat
    49081 aaaaagtaaa tcagcaggct ggaaattata aataatagtt taatatggaa tcagaatttg
    49141 gaaataaaaa gggtgataga taatcaacag caaaataaat cccctcacta gggcaatagt
    49201 gaggggattt ggtgtattta gatgttcaat ttattatgta actaattata gcattgatga
    49261 acaaggcgct caacgtaata ttacaattaa tgga
//
LOCUS       DAPIDM010000006        46951 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000006 DAPIDM010000000
VERSION     DAPIDM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 46951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46951
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.006"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..818
                     /locus_tag="RJE42_000301"
     CDS             <1..818
                     /locus_tag="RJE42_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV5875756.1"
                     /translation="SESLAIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKV
                     IEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLP
                     DKETSFEEYTTMELLSVAMLIVISTFALDFIYTDRSIFKIVDLDEAWSFLQVAQGKTL
                     SMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVD
                     PEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            821..2998
                     /locus_tag="RJE42_000302"
     CDS             821..2998
                     /locus_tag="RJE42_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDV5875757.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSTRTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            2995..3996
                     /locus_tag="RJE42_000303"
     CDS             2995..3996
                     /locus_tag="RJE42_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDV5875758.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            3993..4925
                     /locus_tag="RJE42_000304"
     CDS             3993..4925
                     /locus_tag="RJE42_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV5875759.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5170..5286
                     /locus_tag="RJE42_000305"
     CDS             5170..5286
                     /locus_tag="RJE42_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDV5875760.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5302..7221
                     /gene="tet(M)"
                     /locus_tag="RJE42_000306"
     CDS             5302..7221
                     /gene="tet(M)"
                     /locus_tag="RJE42_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDV5875761.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7340..7507
                     /locus_tag="RJE42_000307"
     CDS             7340..7507
                     /locus_tag="RJE42_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDV5875762.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7567..7920)
                     /locus_tag="RJE42_000308"
     CDS             complement(7567..7920)
                     /locus_tag="RJE42_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5875763.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8425..8847
                     /locus_tag="RJE42_000309"
     CDS             8425..8847
                     /locus_tag="RJE42_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDV5875764.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8844..9074
                     /locus_tag="RJE42_000310"
     CDS             8844..9074
                     /locus_tag="RJE42_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5875765.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9535..9738
                     /locus_tag="RJE42_000311"
     CDS             9535..9738
                     /locus_tag="RJE42_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDV5875766.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9820..11037
                     /locus_tag="RJE42_000312"
     CDS             9820..11037
                     /locus_tag="RJE42_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV5875767.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(11302..11691)
                     /locus_tag="RJE42_000313"
     CDS             complement(11302..11691)
                     /locus_tag="RJE42_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDV5875768.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMVLRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPLSKLFGIG"
     gene            complement(12018..12920)
                     /locus_tag="RJE42_000314"
     CDS             complement(12018..12920)
                     /locus_tag="RJE42_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV5875769.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LSGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(13086..16739)
                     /gene="addA"
                     /locus_tag="RJE42_000315"
     CDS             complement(13086..16739)
                     /gene="addA"
                     /locus_tag="RJE42_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDV5875770.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDCIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPDPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDISYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTKDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIIYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(16740..20216)
                     /gene="addB"
                     /locus_tag="RJE42_000316"
     CDS             complement(16740..20216)
                     /gene="addB"
                     /locus_tag="RJE42_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDV5875771.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYNEQKLSKAAMEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLESFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDALQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTTSYLKSAYLVDLLENFVLERGIYGKRWLDDELFNIEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NINIDDEFGGEQ"
     gene            complement(20376..20951)
                     /gene="lepB"
                     /locus_tag="RJE42_000317"
     CDS             complement(20376..20951)
                     /gene="lepB"
                     /locus_tag="RJE42_000317"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV5875772.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKYNFNPENTKN"
     gene            complement(20967..21491)
                     /gene="lepB"
                     /locus_tag="RJE42_000318"
     CDS             complement(20967..21491)
                     /gene="lepB"
                     /locus_tag="RJE42_000318"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV5875773.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNDIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            complement(21496..22071)
                     /locus_tag="RJE42_000319"
     CDS             complement(21496..22071)
                     /locus_tag="RJE42_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV5875774.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLIVFWIVGKKLEQHFMGSKKE"
     gene            complement(22396..23727)
                     /locus_tag="RJE42_000320"
     CDS             complement(22396..23727)
                     /locus_tag="RJE42_000320"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV5875775.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEDRL"
     gene            24078..25283
                     /locus_tag="RJE42_000321"
     CDS             24078..25283
                     /locus_tag="RJE42_000321"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV5875776.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAVPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            25273..26652
                     /gene="argH"
                     /locus_tag="RJE42_000322"
     CDS             25273..26652
                     /gene="argH"
                     /locus_tag="RJE42_000322"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV5875777.1"
                     /translation="MSNKAWGGRFEVQLEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLADQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLSTYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            26894..27823
                     /locus_tag="RJE42_000323"
     CDS             26894..27823
                     /locus_tag="RJE42_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV5875778.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAIH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(28410..29654)
                     /locus_tag="RJE42_000324"
     CDS             complement(28410..29654)
                     /locus_tag="RJE42_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDV5875779.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(29763..30953)
                     /locus_tag="RJE42_000325"
     CDS             complement(29763..30953)
                     /locus_tag="RJE42_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDV5875780.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVNFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGEYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(31261..32388)
                     /locus_tag="RJE42_000326"
     CDS             complement(31261..32388)
                     /locus_tag="RJE42_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDV5875781.1"
                     /translation="MKSKYEPLFDKVVLPNGVELRNRFVLAPLTHISSNDDGTISDIE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNKRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(32749..33126)
                     /locus_tag="RJE42_000327"
     CDS             complement(32749..33126)
                     /locus_tag="RJE42_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDV5875782.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(33542..34135)
                     /locus_tag="RJE42_000328"
     CDS             complement(33542..34135)
                     /locus_tag="RJE42_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV5875783.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            34199..34582
                     /locus_tag="RJE42_000329"
     CDS             34199..34582
                     /locus_tag="RJE42_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDV5875784.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAHQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            34713..37118
                     /gene="mnhA1"
                     /locus_tag="RJE42_000330"
     CDS             34713..37118
                     /gene="mnhA1"
                     /locus_tag="RJE42_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDV5875785.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTVMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTVFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            37111..37539
                     /gene="mnhB1"
                     /locus_tag="RJE42_000331"
     CDS             37111..37539
                     /gene="mnhB1"
                     /locus_tag="RJE42_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDV5875786.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            37539..37880
                     /gene="mnhC1"
                     /locus_tag="RJE42_000332"
     CDS             37539..37880
                     /gene="mnhC1"
                     /locus_tag="RJE42_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDV5875787.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            37873..39369
                     /gene="mnhD1"
                     /locus_tag="RJE42_000333"
     CDS             37873..39369
                     /gene="mnhD1"
                     /locus_tag="RJE42_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDV5875788.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGIADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            39371..39850
                     /gene="mnhE1"
                     /locus_tag="RJE42_000334"
     CDS             39371..39850
                     /gene="mnhE1"
                     /locus_tag="RJE42_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDV5875789.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            39850..40143
                     /gene="mnhF1"
                     /locus_tag="RJE42_000335"
     CDS             39850..40143
                     /gene="mnhF1"
                     /locus_tag="RJE42_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDV5875790.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            40121..40477
                     /gene="mnhG1"
                     /locus_tag="RJE42_000336"
     CDS             40121..40477
                     /gene="mnhG1"
                     /locus_tag="RJE42_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDV5875791.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            40738..41890
                     /locus_tag="RJE42_000337"
                     /pseudo
     CDS             40738..41890
                     /locus_tag="RJE42_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
     gene            complement(41946..42320)
                     /locus_tag="RJE42_000338"
     CDS             complement(41946..42320)
                     /locus_tag="RJE42_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDV5875792.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(42339..43655)
                     /locus_tag="RJE42_000339"
     CDS             complement(42339..43655)
                     /locus_tag="RJE42_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDV5875793.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTYTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(44066..45541)
                     /locus_tag="RJE42_000340"
     CDS             complement(44066..45541)
                     /locus_tag="RJE42_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDV5875794.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKALFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVEVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(45672..46880)
                     /locus_tag="RJE42_000341"
     CDS             complement(45672..46880)
                     /locus_tag="RJE42_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5875795.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKIETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT        15146 a         8401 c         7640 g        15764 t
ORIGIN      
        1 attctgaatc actggctatt gatattctga cattccttac ggggatttcc tctcgtgatg
       61 gggaacgctt cccaatcctt agaaaagcca ttcgtgcagt aaccaatagt gaagtacgag
      121 ggttgatgaa agtgattgag gaattacggg ttgagaatac gccactaagt accagtatag
      181 ccgaccatat cgaaagtttt acagactatg actttgcaca tttattattc agtaatggtt
      241 atgtggagca gtctatcagc ttagaaaaac aactgaacat tatacaggtt gcggacttgg
      301 tacttcccga caaggaaact tcctttgagg aatataccac tatggagctt ttatccgttg
      361 ctatgctgat tgtcattagt acctttgctt tagactttat ctatacagac cgaagcattt
      421 tcaagattgt agatttagac gaagcatgga gctttttaca ggtagcacaa ggaaaaacac
      481 tatctatgaa gctggttcgg gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc
      541 aaaatacaga cgacctctta gatgaaaaac tgaaaaataa cctcggctta aaatttgcat
      601 ttcgttccac tgaccttaac gagattaaaa agaccttagc cttttttggt gtagacccag
      661 aggacgaaaa caatcagaag cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg
      721 atttatatgg tcgtgtcggt gtgatacagt tccaccctgt atttgaagaa ctgctccatg
      781 cctttgatac cagaccacct gtgcgaaaag aggtgtaaat gtgaaaccat caatagtaaa
      841 cagaataaaa tcaaactgga cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt
      901 cacacttgtg attgccattt ttcttttagc catgctggga acggtggttc aagctgcggg
      961 cttggtagat gatacggtca atgtggcaaa tgaatacagc cgatacccac ttgaaaacta
     1021 tcaactggat ttttatgtgg ataatagctg gggctggctt ccgtggaact ggtcggacgg
     1081 gattggaaaa caggtcatgt atggactata tgccattacc aattttattt ggacaatcag
     1141 tttgtatgtt tccaatgcga caggttactt agtacaggaa gcctattcct tagacttcat
     1201 ttccgctaca gcagattcca ttggtaagaa tatgcagacc ttagctggtg tgagtgcaaa
     1261 cggattttca acagagggtt tctatgttgg attcctctta ctcttgattt tggttcttgg
     1321 ggtttatgtt gcctatacgg gactgataaa gagagaaacc acaaaggcaa ttcatgccat
     1381 tatgaatttt gtgctggtgt ttatcctatc ggcttccttt attgcctacg ctcccgacta
     1441 cattaaaaaa atcaatgact tttcatcaga catcagtaat gccagtttat cacttggcac
     1501 gaagattgtc atgccccatt ccgatagtca aggcaaggac agcgtggact taatcagaga
     1561 tagcctgttt tccatacagg ttcagcaacc gtggctactg cttcaataca acagttcaga
     1621 cattgaaagt atcggtattg accgtgtgga aagcctgctc tccaccagcc cagattccaa
     1681 caatggcgaa gacagagaaa aaattgttgc ggaagaaatt gaagacagaa gcaataccaa
     1741 tctaaccatt acaaagacca ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa
     1801 tattgggatt tccatatttg tattcctatt aacaggaatc atgattttct cgcaggtact
     1861 ttttatcatc tatgctatgt ttctgcctgt gagctttatt ttaagcatga ttccatcatt
     1921 tgatggtatg tcaaaacgag ccataacaaa gctctttaat accattttga cacgagctgg
     1981 aatcacattg attattacga cagcatttag tatttcaacc atgctctata ccttatcggc
     2041 tggttatccg ttctttttga ttgcttttct acagattgtg acctttgcag gaatctactt
     2101 caagctgggc gatttaatga gtatgttttc tctacagagt aacgattctc aaagtgtggg
     2161 aagtcgtgtg atgagaaaac ctcgtatgct tatgcacgct cacatgcacc gtctacagcg
     2221 gaaacttgga cgttccatga ctactctagg ggctgggtct gccattgtta caggtaaaaa
     2281 aggacagtcg ggttcgggga gttctacaag gacacaagca gatcactccc gaccagacgg
     2341 aaaggaaaaa tcaacacttg gaaaacgtat cggtcaaacc atcggtacag tagctgatac
     2401 caaagacaga atggtagaca ctgctagtgg tttgaaagaa caggttaaag atttgccgac
     2461 caatgcaaga tatgcagtat atcaaggaaa atccaaagta aaagagaatg tccgtgattt
     2521 aaccagtagt atttctcaaa ccaaagcgga cagagccagt ggacgcaagg aacagcagga
     2581 acaaaggcga aaaaccattg cgaagcgtcg ctctgaaatg gaacaggtca aacagaaaaa
     2641 acagcctgct tcttctgttc atgaaagacc gactacaaga caagaacaat atcatgatga
     2701 acagacctca aaacagtcta atattcagac ttcatataag gaatctcaac aagccaaaca
     2761 agagcgtcca gcagttaagt ccgatttttc aagtccaaaa gtggaacgcc aaggcaatac
     2821 cgttcaagaa aaaaccgttc aaaagccagc aacttcaacc actacagcag atagaacttc
     2881 acaacgtcca atcacaaaag aacgtccgtc tactgttcaa agagtaccac tacaaaatac
     2941 aagaagtaga ccaccaatca aaaccgccac cattaagaaa gtcggtaaga aaccatgaag
     3001 ttgaaaactt tagtgattgg tggttctgga ttattcttga tggtcttctc actgcttctg
     3061 tttgttgcca ttttattttc agatgaacag gacagcggaa tttccaatat tcattatgga
     3121 ggtgtgaatg tttccgcaga agtgctggct cataagccta tggtagaaaa atatgccaaa
     3181 gaatatggcg ttgaagaata tgtcaacata cttcttgcga ttatacaggt ggaatcgggc
     3241 ggtactgcgg aagatgttat gcagtcctcg gaatccctcg gtcttccacc taattcattg
     3301 agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgaattatt agccagtagc
     3361 gaaaggctca gtgtagattt agaatcggtt atccagtcct acaattatgg tggtggtttc
     3421 ttagggtatg tggctaatcg tggaaataaa tatacctttg aactggctca aagtttctca
     3481 aaagagtatt caggtggcga aaaagtgtct taccccaatc ccatagccat acctatcaat
     3541 gggggctggc gatacaacta tggcaatatg ttttatgtgc aactggtaac gcagtatctt
     3601 gtcacaacag agtttgatga tgatacggta caagccatca tggacgaagc actgaaatat
     3661 gagggctggc gatacgttta cggtggagct tccccgacta cttcttttga ttgtagcgga
     3721 ctgacacaat ggacgtatgg aaaagctgga attaacttac cacgaaccgc acaacagcaa
     3781 tatgatgtga cccagcatat cccactatcg gaagcacaag ctggcgattt ggttttcttt
     3841 cattctacct ataacgctgg ctcttatatt actcatgttg ggatatacct tggcaataac
     3901 cgtatgtttc atgcaggcga cccaatcggt tatgccgact taacaagccc ctactggcaa
     3961 cagcatttag tgggagcagg acgaatcaaa caatgagaaa ggaagattta atgatgaaat
     4021 ttagaaaaaa tcagaataaa gaaaaacaga taccaaagga aaagaaacct cgtgtctata
     4081 aggtcaatcc tcataaaaag gttgtgattg ccttgtgggt acttttaggg cttagtttca
     4141 gctttgcgat attcaagcac tttacagcta tagatactca tactattcac gaaacaacta
     4201 tcatagaaaa ggaatacgtt gatactcatc atgtagaaaa ttttgtagag aactttgcga
     4261 aagtctacta ttcatgggag caatccgata agtccattga taatcgaatg gaaagtctaa
     4321 aaggctatct gacagatgaa cttcaagctc tcaatgttga tacagtacgc aaagatattc
     4381 ctgtatcgtc ttctgtaaga ggatttcaga tatggacggt agagccaact ggcgacaatg
     4441 agtttaatgt aacctacagt gtagaccagc tcattacaga gggagaaaat acaaagaccg
     4501 tccactctgc ttatatagtg agtgtctatg tagatggttc tggaaatatg gtactggtta
     4561 agaatccgac cattaccaac atacctaaga aatcaagtta taaaccaaaa gccattgaaa
     4621 gtgaggggac ggttgattcc attacaacca atgaaatcaa tgagttttta acgacgttct
     4681 tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat gacgggatat
     4741 taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt cacaatcgta
     4801 aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag accaaagcaa
     4861 cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg aagattatag
     4921 aataacaaat attggtacat tattacagct attttgtaat cacgtactct ctttgataaa
     4981 aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta tctatttaaa
     5041 aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg atagtgggaa
     5101 caaataattg gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac
     5161 ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat gcctatggtt
     5221 atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc ccttttgggt
     5281 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
     5341 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
     5401 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
     5461 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
     5521 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
     5581 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
     5641 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
     5701 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
     5761 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
     5821 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
     5881 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
     5941 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
     6001 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
     6061 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
     6121 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
     6181 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
     6241 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     6301 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     6361 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     6421 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     6481 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     6541 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     6601 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     6661 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     6721 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     6781 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     6841 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     6901 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     6961 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     7021 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     7081 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     7141 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     7201 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     7261 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     7321 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     7381 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     7441 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     7501 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     7561 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     7621 ggatagttct ttgtctgtaa agctatccat gtatttctct atctgtaatc gtcgggtgct
     7681 ttttaccaag ttattagcag gtaagaaaaa ttcatcaacg gaaacatgaa gtaacgatac
     7741 aaggtcataa agaacttgta tgctggggtg ttgcccttta ttttcaatat tagttaagta
     7801 ccgtgggtca atttcaatca atgctcccac ttgttcacga gttaaacctc gtttcaatcg
     7861 agcttcttta atggctaaac caaaggctct aaaatcatat ttatcttctt ttttacgcat
     7921 agtagaccac ctctatacat tttattgttc ctactgaatt aaaaacaggt atagaaaaac
     7981 gtgttatatg gtttataggt ttatatttaa taaaaagcac tactaaacgc caataaaaaa
     8041 aaccgttata tggtagtgct atttacgctg ttaaaatatt gtatattact tccaaatggc
     8101 ggtttgttgg aggtcaacgt cgccatgaag tacatcatat acaataaatt tccttacatt
     8161 gggttcttgt caaaaaaagt cgtctatctg caatagataa gtacgtccac caatgtggtt
     8221 ttataaatca tatagataga ataacagaag catgtaaaca gagaaataaa tctgtttata
     8281 tgcttttttg gctattcaga acttttttac aaagtttatt tatcagtaat gcaacaaatc
     8341 cccctttcac attgggacta agagtgaaag gagataaacg agcaaggctc acttcctttc
     8401 ctagacagaa agggggtgag aaacatgaaa ccatcttctt ttcagaccac aatagaaaat
     8461 cagtttgact atatctgtaa acgtgctatg gaagacgagc gaaagaatta tatgctttat
     8521 ctttcaagga ttgcaaagcg tgaggtgtcc ttttcggatg ttggcgatta tcttgttagc
     8581 cagtttgcga caacagataa ctattcaact gactttcaga tttttacact caatgggtta
     8641 tcagtaggcg ttgaaaatga tttgttgagt gaagcattac gtgagttgcc agacaagaaa
     8701 cgtgaaattc tactgctgtt ttactttatg gacatgagcg attcagaaat tgcagacctg
     8761 ttgaaattga accgttctac tgtctatcgg catagaacca gtggactagc cttaattaaa
     8821 aagtttatgg aggaatttga agaatgaaaa cacaatatcc tatgattccc tttcctctca
     8881 ttgtaaaggc aacagatggc gataccgaag cgattaacca gattctacat cattacagag
     8941 ggtacataac gaagcgttcc ctacgactta tgaaagatga atatggcaat caaagtatgg
     9001 tcgttgatga agtcttacgt ggaagaatgg aaaccagact gattacaaag attttgtcat
     9061 ttgaaattaa gtaatatcct ctctcctttc gtggaagcgt gctaaaccat tccacgcttc
     9121 ccgaacaggg aggtttgtta ttccaccaaa gcatattgag ctttcaatgt gttttgatag
     9181 gctaacgagc cattgttctt tgaaaactga ataaaagtaa tcgaatacgt ttcgataaga
     9241 aaagagccaa cggaactaac cgccatgacc tatcttataa agatagcgag cgattcatgt
     9301 tagtgatccg agaagcaatc tttagcagga ttgcctgcaa cgacattctt atcgtgataa
     9361 tgatactccc atacagtcaa tagtccgagc gtgataaaac cgtcgcaggc aatgagtatg
     9421 gctacatgag aaccatgcag gggtggaact cccgtgagct ttgctaaagc tgttcgattg
     9481 ctggtaaaac aacttttatg aaatccaaat aagtgatttg gaaaggagga ttttatgaag
     9541 cagactgaca ttcctatttg ggaacgttat accctaacca ttgaagaagc gtcaaaatat
     9601 tttcgtattg gcgaaaacaa gctacgacgc ttggcagagg aaaataaaaa tgcaaattgg
     9661 ctgattatga atggcaatcg tattcagatt aaacgaaaac aatttgaaaa aattatagat
     9721 acattggacg caatctagcg tcgccaaagg gtcttgtata tgataaaata gtattaagtc
     9781 gtatcaaggc tctttccata aaggaaagga gcaaatgcca tgtcagaaaa aagacgtgac
     9841 aataaaggtc gaatcttaaa gactggagag agccaacgaa aagacggaag atacttatac
     9901 aaatatatag attcatttgg agaaccgcaa tttgtttact cgtggaaact tgtggctaca
     9961 gaccgagtac cagcaggaaa gcgtgattgt atctcactta gagagaaaat cgcagagtta
    10021 cagaaagaca ttcatgatgg tattgatgtt gtaggaaaga aaatgacact ctgccagctt
    10081 tacgcaaaac agaacgctca aagaccaaag gttagaaaaa acactgaaac tggacgcaaa
    10141 tatcttatgg atattttgaa gaaagacaag ttaggtgtaa gaagtattga cagtattaag
    10201 ccatcagacg ctaaagaatg ggctattaga atgagtgaaa atggttatgc ttatcaaacc
    10261 atcaataact acaaacgttc tttaaaggct tcattctata ttgctataca agatgattgt
    10321 gttcggaaga atccatttga ctttcaactg aaagcagttc ttgatgatga tactgtccct
    10381 aagaccgtac taacagaaga acaggaagaa aaactgttag cctttgcaaa agctgataaa
    10441 acctacagca aaaattatga tgaaattctg atactcttaa aaacaggtct tcgtatttca
    10501 gagtttggtg gtttgacact tccagattta gattttgaga atcgtcttgt caatatagac
    10561 catcagctat tgagagatac tgaaattggg tactacattg aaacaccaaa gaccaaaagt
    10621 ggcgaacgtc aagttcctat ggttgaagaa gcctatcaag catttaagcg agtgttagcg
    10681 aatcgaaaga atgataagcg tgttgagatt gatggatata gtgatttcct ctttcttaat
    10741 agaaagaact atccaaaagt ggcaagtgat tacaacggca tgatgaaagg tcttgttaag
    10801 aaatacaata agtataacga ggataaattg ccacacatca ctccacatag tttgcgacat
    10861 acattctgta ccaactatgc aaatgcagga atgaatccaa aggcattaca gtacattatg
    10921 ggacatgcta atatagccat gacgctgaac tattacgcac atgcaacatt cgattctgca
    10981 atggcagaaa tgaaacgctt gaataaagag aagcaacagg agcgtcttgt tgcttagtag
    11041 tacaaatgaa tttactactt atttaccact tctgacagct aagacatgag gaaatatgca
    11101 aagaaacgtg aagtatcttc ctacagtaaa aatactcgaa agcacataga ataaggcttt
    11161 acgagcattt aagaaaatat aaaaagataa ttagaaattt atactttgtt tcttattaaa
    11221 catttttaga atcaaaaaag acataaggtc ttggactaat aattgtccat gccttatgtc
    11281 atatactata tgtcttatca attagccaat accgaataat tttgataaag gtcctaacgg
    11341 tagaatgaca cctaatacca ttgtgatgat aattaatgca attgttatcc aaaacattgt
    11401 gtgactttgt tcatgtctct ttcttttagc aatcgacact tccatcaatc caactactgc
    11461 aacaccacac agcattttca atgtaagcaa catatgattt gccccgccat tcataaatga
    11521 ctgaattaat atccaaaatc ctgaaattaa cgtcaacagc ataaataatc gtagtaccat
    11581 atgtaatggt ttgaaatatg gcgtaccacc ttgatttttt gaaatattta agtatgtagc
    11641 gataaataaa atgattgcta atacccaact taaaatatgt aaatgtaaca tattaattcc
    11701 cccactttaa taattttagt tattaaatta aaacttcgtg ggataaatac ccactttctt
    11761 ataatttaat cattatttgt tcatacacaa atatatattt taccacactt ctatatttta
    11821 gaggaagaac atgacatttt attacttatt aaaatactgt gcatctactg tataataatc
    11881 aactacttct taatatcaat atttcacttt cgtctcaaaa tggtaacatt ataaacaatg
    11941 atctttaaca ccttttaaga aaagcaaaga aaaactaacc aatctatata aagactggtt
    12001 agctttttaa atgataatta tttagcgata taagttgtca gcgttccaat attatcaata
    12061 gtcactttaa cttcatcacc tggttgtaag aatttaggtg gttgcatacc tgcaccaacg
    12121 cctgctggtg taccagttgc aataatatct cccggatgta gtgcaacata ttttgaaatt
    12181 tcttctatta attcatcaat tttaagaatc atttcgccag tgttaccatc ttgtctaatt
    12241 tcattgttaa cttttgtaac aatatttaca ttttcaggta atggtagttc gtctttagta
    12301 acgatataag gacccattgg gcaaccgcca gttaaacttt ttgataaaaa tgcttgatct
    12361 tgttcacttt gtgctttgcg atcagtgata tcgttaataa ttgtatagcc gtaaacataa
    12421 tctaaagcta atgcttttgg aatcttttca ccagacttac caataacaat acctaattca
    12481 ccttcataat ctaattgatc agtaatatct ttatgatttg gaattgttgc attatctcca
    12541 gttaaagatg acgctgcttt tgtaaataca tataattttt ccacttcatg atttaattcg
    12601 ttcgcatgat ctttgtaatt tctaccaaaa gcaatcacat tattcggagg tgttactggt
    12661 ggtaagaatt caatgtcatt aaatgaaatt ttatagtctt cagctttgcc gctatcttct
    12721 gctgctacaa ctgctttacg tacttgttct tgaaaatcta aagtatgatt ttgttgtaaa
    12781 ccagataaca atgttttagg atggaaatct ccttctgcaa agtcagcaaa tacttgtgtt
    12841 aaatcccata cagcatcttc gcgttttact ttaacgccat atgaagtttt gtcattatac
    12901 ttgaatgata agaatttcat tcattctcaa ctcctcatct ttatcttaat tcacattata
    12961 acttttttcg ttatcaaata acaaataaat aagtaagaca attttgaaaa tgagttgtgt
    13021 tcattctgct acaaggactt tgcacttaat cgaaattatt ttttattctt ttgaaaatca
    13081 aaatactata gttgcaatgt accaaatttg aagaagtata aataaccttt aacttcttta
    13141 ttaagaatcg tttgaagcgt attttgataa tatttcatct gtatcttata tttatttttt
    13201 aattgtgtgc caatttcttc atctgtcatc ccacggcgac gattaaatgc atcggtttta
    13261 tagtctacaa aataatgcac accatcttta acaaagatta agtcaatcat accttgaata
    13321 attgagacgt cttcgtctcc ttgtggcaat tggtcaacta atgcttggtt aactacaaac
    13381 ggtaattcac gataaacttg ctctgcttca gcaataatcg aatataactc actattgata
    13441 aatgtcatta tttcatccat acggatatct tttttcgcat ctgcttcgat aatatgttta
    13501 tcgattaatc catcgatata ctgatgtaac tcaacttcag atatgcgttc ttttttgaat
    13561 ggtaaatgtt gcatcactgt atgcattaac gtaccaattt catttgcttt tcgtttacct
    13621 tgttcactta gaaatttagg tcgttcatac gttgaaaacc cgatacgata ttgccttact
    13681 cgttcgtaac ttgtgccact ttcttctgtt tcatattgtc ttttcaattc agaaacagat
    13741 tgttttgagg gctttttagt atcatttaca tatggatatt gataatcaag ttggtgttta
    13801 atttgtgctt taacatcttc attaccattt tgcatagttt ctaattgatt aaccgaacga
    13861 tattcatcat tatctaaaat ggtttctgta gacacatctt caaagtatat aattgaaata
    13921 tttacattcg gacgactact atcttcaatt tgtgctatat ctttttcaaa ttttaaatca
    13981 tctggaattg acgcagattg atgtttagat aaaatactat aaataagatg gaacggattt
    14041 ggtgaagtta atcgttcatt gacagcaatg tgctcaccag aaatagacaa ttgctctagt
    14101 tctagtaacg atttatcatt tttcactcta ccaattaaat aaagttgttc tttcgctctt
    14161 gttaatgcta catagactaa tcgcatttct tctgacacaa gttctttttc ggcaacagct
    14221 ctatatgcaa ccgaagctaa agatggaaat gccatttctt tatccacatc aaaataatcc
    14281 attccgagac caaattgctg atttaaaata actggttgtt tcaaatcacg tttattaaaa
    14341 tcttttgaca atccagaata aatgacaaat ggaaactcta gacctttact actatgaatt
    14401 gtcatcattc taacgacatt atcgtttgga ccaactacgt tttcctcacc aaaatctttg
    14461 cctctttcaa tcaattcatc gataaaacga ataaattgat ataaacctct aaaacttgaa
    14521 ttctcaaact cgatagcttt attaaataaa ccataaagat ttgcacgtcg tccacgtcca
    14581 ccaataagtc cactaaagta ttgaataaca taatgatcat tataaaattt atcaattaac
    14641 tgatacaccg gatgatcttt actatattgt tggtaacttt gaatatctga taaaaacatt
    14701 tttaatttat caactaaaat agcatctgct gcttcgtcat taatgtaatt tacaatcgat
    14761 tgatagaagt agtcatcatt tggactcaat attctaattt gagctaattc gtcttctttg
    14821 aactgatata taacggagcg cattaaccca actaaataaa tatcttgtaa tggattatct
    14881 atcgctctta aaaatgataa tactaaacgg acttctgttt gttcaaagta accttcacga
    14941 ctattcacat ggaatggaat atcttcattt ttaaaggctt gttgtaaatt gcgagcttgt
    15001 ccaaagctgc gttctagaat aacgatatcc ttgtatgtcg cacttctata gcttcctgtt
    15061 ttcatatcat aaactttttg atgttctaag atatctttaa cttgttctac tataaaatgc
    15121 gcttcttgtt cactaccagt taaatcacta tgttcttgat ccgcttcaac aagcactttt
    15181 aagtttactg gatggtccga ttcatcatat ggtgcaccat aatacaactg tgccgcttca
    15241 tcatatttta cttcaccgac ttgttcatcc atcatatgtt tgaatatata gtttgtcgtt
    15301 gacagtactt cttttcgaga acggaagttt tgcgacaaat caattcgacg tccagtgcca
    15361 tctccatctt tagtaaagcg ttgatacttt tcaataaata aacttggatc agcttgtcta
    15421 aatttataaa tggattgctt aacatctcca accataaata aattaccatt atgttcatca
    15481 cccgttttga tacaagataa tattttctct tgaactcggt tcgtatcttg atactcatcg
    15541 accaatattt cttggaagtg ttgacggtat gattcggcaa tttctgaagg cgaaccatcc
    15601 tcattagtta aaatttgtaa tgcaaaatgt tcataatcag aaaaatccaa aatatttttg
    15661 ctacgctttt ttcgattgaa ttctgacata acatctttca caatacgcgc aaggtacttt
    15721 actcgtggcg ccaattgttg catatcagct ttcaaatcat cagcttctct tgaaaagtaa
    15781 tcacttttca ctttatcaat taatgattta tattttttat actgaagttt agcatcttct
    15841 aaggcatcga ccatcatttc atttgcttct ttaattttcg ctgttacatt aggcaaacgc
    15901 gcgccaaatt cgtgaccagt taaataagat atatcaataa agccaccttc taaaacacgc
    15961 cccattaaac gtcgttcatc ttcaataaca gctaaatgtt tatcgacgcc atccatcata
    16021 ctaaacaaat catacgcctt atttaaagca tcataggcag ctgtgataaa tactttagat
    16081 aagtctgtta gtagttgaat aagttgcgct tgttgtgctt cttcttcgta tggtgtcacc
    16141 aattgatcca accaatttgt aggatctgga ttggcaacgc taaagaaata caattgttta
    16201 ataatcattc gaaactgatc atcacttcta tctgaagaca attgctctgt taattcaata
    16261 aaagcaggat caaggatatc gtaatgttgt tctatgactt catctatcgt ttgttctaat
    16321 aataaaatat tttcagcttc actgcttgtt ctaaagttcg ggtcaatatt taatacatca
    16381 taatgctgtt gaattaattt caagcaaaaa ctatgaagtg tagatatttg tgcttgatga
    16441 attttgatgc gttggttttt caagtgtgca tttgcaggat cagcaatcga tgcctcttga
    16501 atacgttggt ctacacgatg cttcatttca cgtgcactta agtttgtaaa cgttacgact
    16561 aaaagtcgat cgacatcaat gcaatcacgt aaaatctttt ggataatgcg ctcaactagt
    16621 acagctgttt tacctgaacc tgccgcggct gcaacaagta catcttgtcc agttgcgtaa
    16681 atactttgcc attgcgcgtc agtccaaatc acgccttgtg gtttctctgg aattgtcatc
    16741 tattgctcac ccccaaattc atcatcaatg ttaatatttt gaattgcttc aattggattt
    16801 attgtttcat ctacagttcg atatcgctta ctatcaatca tgccatctac atgacatacc
    16861 gattgataac tacaaaaagc acatggcaat ttttgtttgt actttaatgg tgcaacttca
    16921 gtatgcccat ccataatatt tgaagctgtt tctataaaat tctctttatt atgttggatg
    16981 aatttataaa tcgttgcttc atctgcaact tggctgcctc gtttactcaa agaaccatct
    17041 ttattcaaac cgactggtac aatatctgaa gtgaatttag gttctaaacg aatatccaat
    17101 gcatcaataa cagtttggtc tgcattcact aaaccactca gcttaaactt tttaattaaa
    17161 tcttgttcta atttatcttc atcaatatct gcccaagatt taaatttaat tctaggttca
    17221 tgtacatgga agtataataa accacctggt ttcacaatat ctgttaatcc aaggcgttgt
    17281 ttattttgta aaacgatatc catatatgtc atcatttgca tttgcatacc ataatatact
    17341 ttcgttaaat caagtgtcgc actaccttca gaggatttat agtcaatgat attaacaaaa
    17401 cttgtatcat tctttgtata cgtatcgata cggtcaattt gccctctaat attaattgga
    17461 ataccttgag ttgtcgttaa tgtttgtgca attaattcgt catttgttct tggtttcctt
    17521 ctaaaacttg tctcaaaatg ttttggcata aactttgaat acgtgccttg atattttaat
    17581 gcgcttagtg ttgtttctac aatagcgcca atgcgtcttg ataaataacg atagtaagct
    17641 gaagaattta ataaattaaa ctgaacttta ggtaaaattt cttccaatgc ttcattcgtt
    17701 aattgtctta tttttttcag gtctaattgt ttaaaatcgc cattaatacg ttcagatata
    17761 tattttaaaa cggaatggaa aatatcgcct aaatcaaagt tttgaagttc atacttcgtt
    17821 cgctcattta gtttcagacc atgtgaggca tagtgtttaa atgggcattg ttgataacct
    17881 tcaaatcgag atacactggc attgatttcc ttaccatata aatctttaga caacgtttca
    17941 cctaatttta cagtttcatt gtcaaacgtt aatgctgaca ttaaataatc taaaccttga
    18001 tttaaatgat cgctatctct aattacttga taagcatcta accaactatc agccacaatt
    18061 tcatcatcta accaagcacg taatgcttca aataatgtaa ttttggtttg cttagcatgt
    18121 tgcattagtg acaatgggtt aacttcatgg tattgaggaa tgttagtaat ttccaattgg
    18181 ttgaacaatg attgaatttg atttaaaaac gggctgattt ccttatcatc accacttgat
    18241 cccattagac tataagaaaa tgtaacatct cccttagctc tagtcatagc aacataacaa
    18301 acaaatgctt catccatctg taaaatatct gatgttggac ttaactcgac attagcctgc
    18361 tgttcaaagt atttcttttc ttcatctgta atcaagcttg acgcagttac tggttgtggc
    18421 atcgtaccat cattcattcc aactaagtaa acatgttgct tattgtcgac tttagccaaa
    18481 tccatcgtac caatactaac ttggtccaat gtttgcggaa tcataacaaa ttctaattgt
    18541 tctaaaccaa tatcaaatac ttctaagaaa cgttccatcg acattggttc atctccaaat
    18601 actagaacta agtcatcaag gatttgaatt aagccattcc atatttgatc aatttcttcc
    18661 gccttttcat gattaccatt taaatcaagt tcatctcgct ctgtcatcaa ttgatttggc
    18721 agttcaaaat attccatact ttcataaaaa gcagttgcaa agtcttttac agtttccgct
    18781 tgtgacattt gcttttcaaa atgtaaaatt ttatcaatga catctttctt taacttaacg
    18841 acttgttcaa atgtgttacg ttcatcttcg gtcagtttat gcgctttacg ccccattttg
    18901 ctaaaatgtt cgatattaaa tagctcatca tctaaccaac gtttaccgta tataccacgt
    18961 tcaagtacaa aattttcaag taaatcaact aagtatgcac tttttagata tgatgtcgtt
    19021 aacacatcag tcttcaataa gcgtagcatt ggattaactt gccaattaga ttgaataact
    19081 tcaatcaatg aacgaatcat ttccatgacc ggatgatgtg tcatcgaacg ctttgtatca
    19141 atgttataag gaatattata aagcggtaat atggaatcaa ataaataagc ataagactcg
    19201 tcacgatata aaatagcaat atcttgatat cgtaattgct tatcacgaat atcaacgatg
    19261 atacgtcgtg caatttcatt tatttcctct ctcatagtcg cagattctaa aatattgata
    19321 tgaccttgac atgccactcg attgatttga agcgcatcaa attcttgttc aagatgcttt
    19381 aaatcttgat tattaaagcg atataattgg ttgaaatatt gacgttcaat agaaatattg
    19441 agttcatttg ctatttcttc aatatgtcgt aacacttccg atggttttct aaataaactg
    19501 aattgatcgt ggttaccatc tgtcgtcaat ataattgtga cactcttcgc atatttaatt
    19561 aatcctttga ttattaaata ctcaatcgtt gaaaagttgt gaaaaccatc aatatatata
    19621 tcagcacgtt ttagccactc tgatttcggc atacaatcaa taaaatattg taatgcatcc
    19681 tcacccgtaa taaactcgtt ttgaatgcgt tgttcgaact cacggtatat taaagcaata
    19741 tcctctaact tatttttagt tcgagtttgc atatttttat cagcaataaa gctttctaaa
    19801 tgttcaggcg ttactgcata ttttttaaaa tcttgaattt gttctgttaa tttttcacta
    19861 aacccataat attttgcttg tgattgataa agttttaaat acttttgttg ttcttgaaca
    19921 atgttataaa tcatcatttc catagcagct ttagatagtt tttgttcatt ataactacca
    19981 acttcttgga aaatacgatg acttaatcgt tcaaaatgca acacttctgt tcttaaacta
    20041 ccatttaatt ccggatcatt gacaaaggct tgttctaatt gaaatgtact ttgagttggc
    20101 gcaattaaaa tgattggatc tccaagcgga tctgctttca ttttttgttt tatttcggtc
    20161 aacattttcg tagacttacc tgttcccgct ctacctaaat aagcatgtaa tgtcatgaca
    20221 aaccctcact tcctttgaca catattttaa cacaaagtga ttgtgattga tttattataa
    20281 gttcatatcg aactttactt ttattgtggt actaacaaaa taaaacatcg tttatcagta
    20341 ttaaaaccga caaacgatgt tgtatttgtt tcatattaat ttttagtatt ttcaggattg
    20401 aaattatatt taaattcgct gaatggccag aatctaaatg aaactttacc aacaatttgg
    20461 tcttcatcaa tgaggccaaa cgcacggcta tctttactta cttcacgatt atccccaaga
    20521 actaaatatt taccttttgg aatgacattt gatttagggt tcgcattcgg taaatcttta
    20581 acttggaaag tcccagtaat gtaatcacct tgtttatgtt ttaaattgta gtttaaatat
    20641 ggttcatctt gttttttgcc attgacatat aatgtatcat ttttatattc tactttatca
    20701 ccaggaacac cgatgacacg tttaacatag tcatcatttt tgtttgcatg gaagacaact
    20761 acattacctt tttccaaacc acctgtttta tatccaataa tgtttacagc tactcgctcg
    20821 ccatctttca aagttggatc cattgattca cctttaattg tatatggtgt aacaataaat
    20881 ttacctacta taaataaaat gacaaaagcg actgcaattg aaataatcca ttccaataat
    20941 tcttttttca attttgacac ctctttttaa gatttgaact gaatcgtcca ttttgaaaaa
    21001 ggataatatc tcaaacttat attaccaata atatcctttt tatcaattaa accaaattgt
    21061 ctagaatcat gctgattgtt atcatgatca tttagcacaa caaaattgtt aggcggtata
    21121 atatctccat ctaattcttt aaaattgcgc aaactaaaat ctttaatttt tctgttcttg
    21181 gcataagatg cgtcaaccgg tcggtcatca cggtataatt gtccctgacg aaacgccatt
    21241 gattgaccag gtttggcaat aattcgacta gtatatatat cgttaccacg cctatatgta
    21301 atgatatcac cattattcaa ttgattaaat gtaactttaa ttttatttac aataacacga
    21361 tcccctttgt taagggttgg tgacatatca ttattcggaa tgacatgacc aactattaca
    21421 aaagtttgta cgaacagtac aatgataata gcaagtatca atgaaatcaa atattttaca
    21481 acttttttca cgatgtcact cctttttcga ccccataaaa tgctgttcta acttttttcc
    21541 aacaatccaa aatacaatca acacaactaa cattaatatc cctcttaaag gatgcgttaa
    21601 aattgtagta atttcctttc ctaaataacc taaaataatt gttgaaacta actttgatga
    21661 tgctaaaaca atgaaataat atttaggtct aatatgagat agactcgcta caaaatttat
    21721 taatgtattt ggcgtaaaag gaaaacaaag taaaataaac aatgggatta atccttggcg
    21781 atcaataaaa ctaatcaagc gttgaacagc agtacgttgt ttaattcgct gcatcctttc
    21841 agtgttcacc aatcgtttac agatcaaata gactgtaaat gttccagaaa ttaatccaag
    21901 ccaactaatc aatataccta aaataggtcc ataggcttga atattaatta aaatatagag
    21961 tgctaatgga aatactggaa taatcgctct tatatataac aatatgaatc caggtaaata
    22021 accaaactgt cgaaatatct caaaccattc ttctacttga tgaaacgaca aatcatcaat
    22081 ccctttcttt ggctgaagat aattattctt acattataaa gttaattact aagtaaagaa
    22141 acattaacat gtaatcatgc taataaatca gtctctagta gtataacaat attattgtat
    22201 ggcagatatt attttatctt taattttctc ttaacatctt gtcacattag ctagtaacct
    22261 agttcttgca tataaataaa aacgaggttg tcattacatt tttacatttg taatgacaac
    22321 ctcggaaata tgctttaaat agttaacatc tagttctatc ttttcaaact ttactaatca
    22381 ttgaagtaat atattttata aacgatcttc taattctttt ttcaagtctt caaaaccagg
    22441 tttacctaat aatgcgaaca tattttgttt atatgcttct acaccaggtt ggttaaatgg
    22501 atttacgcct aattggtatc cactcattgc acaagcaagt tcgaagaagt atacgacata
    22561 accgaaagtt tcttcatcta attgtggaat gttcactacc atgttaggaa caccaccatc
    22621 agtatgcgct aataatgtac cttcgaatgc ttttgtgtta acttcgtcga ttgttttacc
    22681 agctaaataa tttaatccgt ctagatcatc actatctttt tcaatagtaa tatcatattt
    22741 aggatgattt acttttacaa ctgtttcgaa taagaaacga cggccttctt gtacatattg
    22801 acctaaagaa tgtaaatcag ttgtgtagtt ggcacttgaa ggatagatac ctttgaagtc
    22861 tttaccttct gattcaccaa ataattgttt ccaccattca ttaaagtatt gcatagatgg
    22921 ttcatagtta atcaacattt ctgttgtata accttttgca tataaaatgt ttcgaattgt
    22981 cgcatattgg tatgcaatgt tttcttctaa tttatctgaa gataattctt cacgtgcttt
    23041 tgcagcacca atcatcatag cttcgatgtt aattccagct gttgcaattg gtaataatcc
    23101 tactgctgtt aaaacagaat atcttccacc tacatcatca ggtacgatga acgtttcata
    23161 accttcgttt gtagccaact gttttaaagc acctttttct ttatccgttg ttgcaaatat
    23221 acgtttttgt gcttcttctt taccgtatct ttcttcaact aattgtttga acaatctaaa
    23281 tgcaactgct ggttctgtag ttgtaccaga tttagaaata acgtttacag agaaatcttt
    23341 gtctgctaaa taatcaacta actctttagt gtatgttgat gataagtgat taccaacaaa
    23401 tacaatttca gggtattcat tgctgtttct aaatgatgac gttaacattt cgattgctgc
    23461 acgtgcacct aagtaagaac caccaatacc gatgactact aaaacatcag aattttcttt
    23521 aatgcgtttt gatgcttcaa caattcttga aaattcttct ttgtcgtaat caactggtaa
    23581 atcaacccag cctaagaagt cactacctgc accagtacct tcatgaattg ttttgtgaat
    23641 tgatttaaca atttcttgtt gttgttttaa ttcgtgttca ccgaaaaatt ctaacgtttt
    23701 actaaaatct aattgaatat gagtcatcta taaaagcctc ctgtttcatt taatatctta
    23761 attttactaa gtttacgttt gcgattcaat tcacaagttt gtacttaatc aataaaattg
    23821 atatttatgt gacgtttcac acctattttt caacaaacat ttcaaactgg cgtcaaacac
    23881 ccaaacattt tttatacaat ttgcatgttt tttaaaacac ttttaaatat tgataaataa
    23941 gcatttatac ttacaactct aactacctct atcgcatttt atgaataaat atgtaaaata
    24001 atggttttat tttcattaaa agtttgctat catgtgttca tacgaaatat tcagataact
    24061 acaatgaggt gaattttatg aaagagaaaa ttgttttagc atattcagga ggactagata
    24121 caagtgtggc cgttcaatgg cttatcgaca aaggatacga cgttgtagct tgttgcctag
    24181 atgttggtga aggtaaagat ttagacatcg tttataaaaa agctttagat atgggagctg
    24241 ttgaatgtca tattattgat gccacaaaag aatttagtga tgagtatgta agttatgcaa
    24301 tcaaaggaaa tttaatgtat gaaaatgcat atccattagt ttctgcttta tcaagaccat
    24361 taatcgctaa aaaattagta gagattgctg agaaaacaaa ttcagtaggt attgcacatg
    24421 gttgtactgg taaagggaat gaccaagtac gtttcgaagt tgccattaaa gcattaaacc
    24481 catctttgaa agcattcgca cctgtgcgtg agtgggcatg gagccgtgaa gaagaaattg
    24541 attatgcaat taaacataat atccctgtat caatcaatca tgattcacct tattctatcg
    24601 atcaaaacct atggggcaga gcgaatgaat gtggtatttt agaagatcct tatgctgtgc
    24661 caccagagga tgcgttcgat ctaacaaatg ctttagaaga aacaccagat actgctgatg
    24721 aaatcatttt aacgtttgat aaaggtatcc cagttcaaat tgatggcaaa acatatgaat
    24781 tagacgattt aattttaacg ttgaatgcat tagctggtaa gcatggtatc ggaagaattg
    24841 accatgtaga aaatagactt gtaggtatca aatcaagaga aatttatgaa gcacctgctg
    24901 cagaagttat tttaaaagca cataaagcat tagaaacgat tacgttaacg aaagatgttg
    24961 cacactttaa accaatcatt gagaagcaat ttgctgaaca actatacaat ggactttggt
    25021 tctcaccttt aactgatagc ttgaaattat ttattgatag tactcagcaa tacgtaagtg
    25081 gtgatgtcag aattaaatta ttcaaaggta atgctatcgt gaatggtaga aaatcacctt
    25141 acacattata tgatgaaaaa ttagcaactt atacaaaaga agatgcattt aatcaagacg
    25201 ctgctgttgg ctttatcgat atctatggtt tacctactca agtaaatgcg atgttacatg
    25261 gaggctatag caatgagcaa taaagcttgg ggcggtagat ttgaagtaca acttgaagag
    25321 tgggttgatg actttaacgc atccattact tttgatcaaa cgctcataga tcaagatatt
    25381 gaaggcagca ttgcgcatgc aactatgctt gcggatcaag gcattattag tcaacaagac
    25441 agcgaacaaa ttatacaagg actaaaatct attcaacatg attatcatca agatcaaatt
    25501 caatttagtg catcattaga agatattcat ttaaatattg aacatgaatt aattaaacgt
    25561 attggtgacg ctggtggtaa gttgcatact ggacgcagta gaaacgatca agttgcaaca
    25621 gacatgcact tgtacactaa gaaacaagtg caagatatca tcgcattaat taagtcgtta
    25681 caaagtgtaa ttgtagacat cgcttccaat aatgttgata caattatgcc tggttatact
    25741 catttacagc gtgcacagcc aatttcattt gcacatcata ttatgactta tttttggatg
    25801 ttacaacgag accaacaacg atttgaagat agtttaaaac gaatcgatat taatccttta
    25861 ggtgcagcag ccttaagtgg taccacatac cctatcgata gacacgagac aacagcattg
    25921 ttgaactttg gcagtctcta tgagaatagc ctagatgctg ttagtgacag agactatatt
    25981 attgaaacat tgcataatat ttctttaacg atggttcact tatcacgctt tgcagaggaa
    26041 attattttct ggtccacaga cgaagctaaa ttcattacat tatcagatgc attttcaact
    26101 ggctcatcta ttatgccaca aaagaaaaat cctgatatgg cagaattaat tagaggtaaa
    26161 gttggccgaa cgactggtca tttaatgagc atgcttatga ctttaaaagg attacctctt
    26221 gcatataaca aagatatgca ggaagataaa gaaggtttat tcgatgcagt tcatacaatt
    26281 aaaggttctt tacgtatctt tgaaggtatg attcaaacga tgacaattaa taaagaacga
    26341 ctcaatcaaa ctgttaaaga agatttttca aatgcaacgg aactagcaga ttatttagta
    26401 actaaaaata ttccatttag aactgcacat gaaattgtag gaaaaatcgt cttagaatgt
    26461 atacaacaag gtcattattt attagatgtt cctttatcaa catatcaaca acatcattct
    26521 agtatcgatg ccgatattta cgattatttg cagcctgaaa attgcttaaa acgacgtcaa
    26581 agttacggtt ctacgggtca atcatcggtc aaacaacaac ttgatgttgc taaacaatta
    26641 ctatcacaat aaatacatta atctacctac ccacaatgtc tatgaaaatt acattgtggg
    26701 tattttaatg ctcttttcgt cttattgaac atcacatttt taagattcct aaaatgtttg
    26761 ataattcttt taaatttata ttacagaaat gttataaatt gtaaaagaaa tgtgtaaagc
    26821 gttttcacaa gcaggttttt gtagtatttt aaaattgtta gactacaaat aaagagatga
    26881 aaggataaag actatgacta actcttcgaa aagcttcact aaatttatgg ctgcttctgc
    26941 tgtttttact atgggatttt tatcagtacc tactgctggc gctgaacaaa caaatcaaat
    27001 tgcaaataaa cctcaggcta ttcaatggca tacaaattta acgaatgagc gattcactac
    27061 tatcgcacat cgtggcgcaa gtggctatgc acccgagcat acgtttcaag catatgataa
    27121 gagtcataat gagttaaaag catcttatat cgaaattgat ttacaacgta ccaaagatgg
    27181 ccatttagtt gctattcatg atgaaactgt taaccgtaca acaaatggac acggtaaagt
    27241 tgaggattat acccttgatg aattaaaaca gttagatgca ggaagttggt ttaataaaaa
    27301 atatccaaaa tacgcaagag caagttataa aaatgctaaa gtacccactt tagatgaaat
    27361 tttagaacgt tatggtccga atgcaaacta ttatattgaa acaaagtcac ctgatgtata
    27421 cccaggaatg gaagaacaat tattagcttc attgaaaaag catcaccttt taaataacaa
    27481 taaattaaaa aatggacatg taatgattca atcattttct gacgaaagtt taaagaaaat
    27541 tcatcgtcaa aataagcatg tgccattagt aaaattagtt gataaaggtg aactacaaca
    27601 atttaacgac caacgcttaa aagagatacg ctcttatgcg attggattag gtcctgatta
    27661 tacagattta actgaacaaa atacccatca tttaaaagac ttaggattta tagtacatcc
    27721 ttatacagta aatgaaaaag ctgatatgtt acgattaaat aaatacggcg ttgatggtgt
    27781 ctttacaaat tttgctgata aatataaaga agtcattaaa tagtattgtt aaactagaaa
    27841 acataaatac aaaaatatag ctattactat aaaaaacagc agtaagatat ttccgaattg
    27901 aaattatcct actgctgtct ttttgggagt gggacagaaa tgatattttc gcaaaattta
    27961 tttcgtcgtc ccaccccaac ttgcacatta ttgtaagctg acttttcgtc agcttctgtg
    28021 ttggggccca caccccaact tgcattgcct gtagaatttc ttttcgaaat tctctgtgtt
    28081 ggggcccctg actataattg aaaaagcttg ttacaaacgc atttttgttc agtcaactac
    28141 tgccaatata acttcgtaga gcatagaaca ttgatttatg tcccagcctg gtttaattag
    28201 aaattctctg tgttcagggc cccgccccaa cttgcacatt attgtaagct ggctttccac
    28261 cagcttctgt gttggggccc cgccaacttg cattgaaaag agcgtgttac acgggcattg
    28321 ttttaccatc aactactgct aaaataaaat taacgagctt aggactttgt tttctgtccc
    28381 aagctcgtta aatcacatac gataattaat tatgcccaac cacgatatct agctgcttct
    28441 gctgtacgtt taatacctat gatatatgct gcaagtctca tatctatttt tcgattttga
    28501 gacaattcgt aaatcgtatc aaatgccgct tctaattttt cacgtagctt ttcattaact
    28561 tcttcttcag accaataata accttgatta ttttgtaccc attcgaagta agaaaccgtt
    28621 acaccaccag cacttgctaa tacgtctgga actaataata taccacgttc agttaaaata
    28681 cgtgttgctt ctggtgttgt aggtccatta gcagcttcaa caacgatact agctttaata
    28741 tcatgtgcat tgtcttctgt aatttggttt gaaatagccg ctggtactaa aatgtcacaa
    28801 tctaattcaa acaattcttt atttgagatt gtttcttcaa ataaatttgt taccgtacca
    28861 aaactatcac gacggtctaa taaataatct atatctaagc catttggatc gtgtaatgca
    28921 ccgtaagcat cagagatacc tacaattttt gcacctaaat catataagaa tttagctaag
    28981 aaacttccgg cattaccgaa accttgaata acaaccttgg caccttcaat ttgcatatta
    29041 cgacgttttg cagcttgttc aattgcaata actacaccta gtgcagttga tctgtcgcgt
    29101 ccatgagaac cacccaatac aattggttta cctgtgatga aacctggtga attaaattta
    29161 tctaatgcac tatattcatc catcatccaa gccataattt gtgagtttgt aaatacatct
    29221 ggtgctggaa tatctttgtt cggacctacg aattgtgaaa ttgctcttac atatccgcgt
    29281 gataaacgtt caacttcatg aatgctcatt tgacgtggat cacaaacgat accaccctta
    29341 ccaccaccgt atggtaagtt aacaatgcca catttcaaag tcatccacat tgataatgct
    29401 tttacttctt cttcatcaac atctgggtgg aaacgcacgc ccccttttgt tggtccaaca
    29461 gcatcattat gttgcgcacg gtaacctgtg aatgttttaa cagtgccatc atccattcgt
    29521 acagggatac gcacttgtaa cattcttaaa ggttctttaa ttaaatcgta cattccttcg
    29581 tcaaatccca atttatgcaa tgcttcttta ataattcctt gagtagaagt tactaaatta
    29641 ttgttctcag tcatgatcct tttcgcctct tctttaccta atgatttcgc tttcaaacat
    29701 attgtaacat aacgtattcc tttttaaagc ccttacaaac tgattgttac aactttttga
    29761 cattattgaa atacatgtct tattttttca agtgcaaggt ccaattcttc cttagtaata
    29821 attaatggtg gtgcaaaacg aatgacagta tcatgcgttt ctttacataa taagccttct
    29881 tcttttaatg cttcacagta tggcctagca ctttcattaa gttccacacc tataaacaaa
    29941 ccacgtccac ggacttcttt aattgatgga tgatcaattt gttttaattg ttctttaaaa
    30001 tactctccta attctaaaga gcgtcctggt aaatcttcat caacgataac atctaatgca
    30061 gcaattgatg cagcacaagc aagtggatta ccaccaaatg ttgaaccatg tgagccaggt
    30121 gtaaagacat ctaatacttc tttatctgct aatacaacag aaattgggaa gacaccacca
    30181 cctagtgctt tacctaaaat atagacatca ggttttacat tatcccaatc cgtagcaaat
    30241 aatttacccg aacgacctaa tcctgcttgg atttcgtcag caataaataa gacattatgt
    30301 tcatcacata attctctaat tgctttcaaa tatccttctg gcggtatatt tatacccgct
    30361 tcaccttgaa ttggttctac taaaactgct gcagtatttt cattaattgc agctttcaat
    30421 gcatctacat ctccaaaatt aacttttcta aatccatcta ataacggacc ataaccacgt
    30481 tggtattctg cttctgatga taatgaaact ggcgccattg ttcgaccatg gaagttacca
    30541 ttaaatgcaa tgatttctgc tttatttggc tcaattcctt taacatcgta tgcccagcgt
    30601 cgtgctgctt tcaaagctgt ttctactgct tcagcacctg tattcattgg taaggcttta
    30661 tctttacctg ccagtttaca aattttttcg taccattctc ctaagttatc actatgaaaa
    30721 gcacgtgaaa ctagagtcac tttatcagct tgatctttta atgcttgaat aattttcgga
    30781 tgtctatgac cttggttaac agcggaatat gcagataaca tatccatata tttattgcct
    30841 tcaggatctt taacccatac cccttcagct tctgaaatga caattggcaa tggtaaataa
    30901 ttatgtgctc cgtaatgatt tgttaactca ataatttttt cagatttagt catcatatct
    30961 ccccttttca tcatttataa ctattataca tgaaacatta tccaaataat tacattagtt
    31021 ttcaaagcag atactttttc acccgaaaag atgaattaat cactaagttt cattaaattt
    31081 gtctattttg aaaaccctta catttataat gacataatta cttaaatgat tacaagcaaa
    31141 agaattgata attttacact taatcaaaag tatattttac taagaatatt tttatttata
    31201 aatattgaaa accactaaca aattgcatac acaatatcat tagtggtaac agttaaacac
    31261 ttatttatct ttacgtggca atggatagaa tccttcatta aattgatgcc ataaattagg
    31321 agttaagtga tgattatctt ctctctctgg atcaaagtaa ttaataattt catcttcccg
    31381 tccatcttta attttttcaa caaattgata atccagtagt agctctctac caatggctac
    31441 taagtcaaca ccaacatttt caactgcatc taaagcttcg tcagctgtga aaattgaacc
    31501 aataccgata agtggcatac gaccatttat ccatttgtga attaaaggca gtctttcttg
    31561 tccagcgtat ttaccttcac gtgtcgttgc atgcgtatcc atcattgaaa catgaatata
    31621 gtcgattggc atatggctaa ttttattaac gagttcctct gtaatttcca ttgtgattcc
    31681 tggagactcc gcttcctctg gagataatct gtatccaact ataaagtctt tattgccata
    31741 cgcttctttc gctttaagca cttcttcaat cacagcgacc gggaatttat attgatttgc
    31801 ccatacatca tttcttttat tatagtatgg tgatacaaat tgatgaatta agtaatgatt
    31861 cgcgccatgt atttcaacac catcaaaccc tgcttcaatt gcacgtcgcg ttgcttcacc
    31921 aaaatccttg atagcttgtt caatctcttc attcgtcatt tctctagcac tatgttcttg
    31981 tttctgaccg aaacttttta aagaaatcgg acttggtgct acgacgtctc catcaggtgt
    32041 taattcaggc aatgcttgtg caccgccatg atgtatttgt acgagtgctt tggcaccgtt
    32101 tttcttcatt gctgtagcta atcgttttag tccttcaata tcactgtcat gcgcgattga
    32161 tggctgtcct ggaaatgctt ttccgacatc actcacatta ctcgcagcat taattgtaat
    32221 accaacatct tgtgagcgct tttcaatata aggaagttct atatctgaaa tagtaccatc
    32281 atcatttgaa gaaatatgtg ttaaaggggc taacacaaat cgatttctca actctactcc
    32341 atttggtaat actactttat caaacaatgg ttcgtattta cttttcatta cttaattcct
    32401 ccttaaaatt attgagttga ctataacatg taattttttc attctccaat tatctgttta
    32461 tatagttgca attcgtttat actttaagtt gtagttctca tttctaaaat aaattaaaaa
    32521 aacaaatcca catacatcat tgcaaatcaa cgattctatc taacaaagat aatgcatatg
    32581 tcaccttaaa ccatttgtac gtgtaaatag aatcaatcat attacgttat cgtatacaaa
    32641 aagagattag tatctaataa aatttaatag atactaatct cttttatagt ctaagaaact
    32701 ttcatttcag accctttgta tgattttcgg tacgatttat ctgttccttt agtcgtttcg
    32761 aattgctcgc tttgactgtt ttatccagat tggtaagcgt tctttaagtg tttgaaaccc
    32821 atatttttct gtttctctga tttcatctaa tactgattgt ttttccttct tacgctcata
    32881 atttttgaag taatcattat cctttaatga tagattgagc tttccttcat catctataga
    32941 caaaatttta gctttaacaa tttggccttc tgatagaaat ttcttcaaat tatgaacgta
    33001 gtcatccata atttctgata tatgaatcag tccttcagta tgattagggg tctcaacaaa
    33061 cgcaccgtaa ggttgaatac cagttacacg caccttgata tgttggccaa ttttgtagtt
    33121 attcaactta ataacccctt atttcgttta cttaacacta ttatcatagc acaaatcgaa
    33181 atggtggact aattaaaaca tcaaatcata cgaatattat tttataaatg aataagaata
    33241 ctacaaactt ggatttacaa ataaaatcag caacaaattc ctatctaaat caatatgata
    33301 tacagctact ttgttttcaa tttattaact atttgaatag caattctctg taaaaacata
    33361 aaaagcaacc tcaactatta tctttagaat acacatctat ttaaaaactg tgctttcttg
    33421 ataaatatat tgagattgct catcatgtta gtttatttgt tttcgttgga atatatacta
    33481 atccattcac aaattagctt tatccgagtt taaaatgcta gtagttaatt atgtttagat
    33541 attattcttc aacatcaata gattcaatta caacatcatg aagtggtttg tcttgtggtc
    33601 ccacttttgt atttgcaata tcttctaaag tagtttcacc atcaatgatt tgaccgaata
    33661 ctgtatgttt ttgatctaac catggtgtac cacccttctc gccatatgca tcaacgattg
    33721 gttgaggcca accaccatct gcaagttgac ttaacatatt ttgaggtacc tctttcattt
    33781 gaacaatgaa aaattgtgaa ccatttgtat taggtcctga gttagccatt gataatgcgc
    33841 catataagtt aaatgcattt aatgaaaatt catcttcaaa agcaccgcca taaatacttt
    33901 cgccacccat accagtagct gttggatcgc caccttgaat catgaagtca ttaatgacac
    33961 ggtggaatgt tattccatca taataaccat tttttgcatg tgtcacaaaa ttttcaactg
    34021 tttttggtgc aatatttgga aataatttga atgtcatgtc acctttattt gtgtgcatga
    34081 ccactttaat ttcaccttgt tgtacttctt tgtttaactg tggatagtta gccatttata
    34141 ttctccattc atgttatgat acatatatat taacatactt ttaagggagg aaaatagcat
    34201 gaagctatat cgattttcac acaaaacagg tagctatggt gtcacgattg ttgaagaaaa
    34261 tggagacgat gtacttgtta aagttgaaca agttatcaaa cacccgaaac aaggtgattt
    34321 acataaccct actgaaactg aaggtgtatt tttccatgaa cgcaaagcat taagtcattt
    34381 tgaaaaacgc tatacgaaac gttcacaatt acgcgacttc aatgttgatg tattgcgcta
    34441 tgaagattca cttcaacaag caattacaga tttagaaact aaacttaaag ctcatcaaac
    34501 aaaacatgct gaaatgtctt tagctagttt agcacgctta aaagaagatt attcacttca
    34561 atacaaagaa aattttaaat aaaaaatgac gtgtttcttc ttatttaaat acttctgaaa
    34621 ataaagcaca acaaggagtg tgtacgtttt tcagaactat atcattcatg tataatgaat
    34681 aacataaata ttttttaaag gaggacaaca tagtgagttt gttacatatt gcggtgatat
    34741 taccgttaat attcgcactc attattccta tcctgtatag attttttaaa cgtatacatt
    34801 taggatggtt cgtattacct gtccctatcg tcatatttat ttatatgctt acattaatta
    34861 aaacgacgat gtcaggtaat actgtaatga aaaccttaaa ttggatgcct cattttggta
    34921 tgaactttga tttatatctt gatggcttag gtttactctt tagtttgctt atttcaggaa
    34981 tcggcagttt agtagtatta tattcaatcg gctacttaag taagtcagaa caactaggga
    35041 atttttattg ttatttatta ctatttatgg gtgcaatgtt aggtgtcgta ctttcagata
    35101 atgttattat tctatattta ttctgggagc taacgtcatt ttctagtttt ttacttattt
    35161 cattctggcg cgaacgacaa gcatctatat atggtgctca aaaatcgctc ataatcacag
    35221 tatttggtgg tctatcatta ttaggtggta tcatcttact cgctatacca acgcaatcat
    35281 ttagcattca gtacatgatt caacatgctt ctgaaattca aaatagccca ttcttcattt
    35341 ttgcgatgat tttaatcatg attggtgctt ttacaaaatc tgctcagttt ccattttata
    35401 tttggctacc agatgcaatg gaagcaccta caccagtcag tgcttatctt cattcagcaa
    35461 caatggttaa agcagggtta tatttaatcg ctcgaatgac accaatcttc gcagcatctc
    35521 aaggttggat atggactgtc acactcgttg gtttaattac gttattctgg gcttcgctaa
    35581 acgcaacaaa acaacaagac ttgaaaggta tactggcatt ttcaacagta tcccaattag
    35641 gtatgattat ggcgatgctt ggaatcggtg ccattagtta tcactatcaa ggcgatgata
    35701 gcaaaatata tgctgctgca tttacggctg caatattcca tttaatcaac catgcaacat
    35761 ttaaaggtgc cctatttatg attacaggtg cagtggatca ttctactggt acacgtgatg
    35821 ttaagaaatt aggtggctta ctaacagtta tgcctatttc atttacaatc actgttatta
    35881 cagcattaag tatggctggt gtaccaccgt tcaatggttt cttatcaaaa gaatcatttt
    35941 tagaaacaac atttacagca agccaagcaa atttattcag tgttgataca ttaggctacc
    36001 tattcccaat cattggtata gtgggcagtg tcttcacatt tgtatattca attaaattta
    36061 taatgcatat atttttcgga caatataaac ctgaacaatt acctaaaaaa gcacacgaag
    36121 tatcaatatt aatgctttta tcaccagcta ttttagctac attagtaatt gtatttggtt
    36181 tattccctgg cattttaact aattcgatta ttgaaccagc tacatcttcg attaatcata
    36241 cggtaattga tgatgttgaa ttccatatgt tccatggatt aacgcctgcc tttttatcaa
    36301 cattagtgat ttatatttta ggtatcttac taattgtgac atttagttat tgggtgaaat
    36361 tgttgcaacg acaacctggt aaactaacat tcaattattg gtacaacaga agtgcaaatg
    36421 ttattccaaa ctactctgaa aagatgacga atagctatgt taccgattat tcacgtaata
    36481 atttagttat catttttggt gcattaattt tattaacatt tgtcactgta ttctcagtac
    36541 ctttcaacat taattttaaa gacgttagtc cgattagaat tttcgaagtt tgtatcgtaa
    36601 ttttattatt aagtgctgcc tttttaattc ttttcgctaa atcaagatta tttagcatta
    36661 ttatgttaag tgcagttgga tatgctgtgt ctgtattgtt tatattcttt aaagcacccg
    36721 acttagcatt gacacagttt gtcgttgaat ctatttcaac agcattgttc ttattatgct
    36781 tctaccactt accaaattta aatcgttata atgaaaagcg ctcattccag cttactaatg
    36841 ctttaattgc tggtggtgtt ggtttgtccg tcattattat cggtttaatt gcatacggaa
    36901 atagacattt tgaatcgata tctaagtttt atcaagagca cgtttatgat ttagctcacg
    36961 gtaaaaacat ggtaaacgtt atacttgttg atttccgtgg tatggatact ttattcgagt
    37021 catctgtgct tggtatcgca ggattagctg tatatacaat gattaaatta cgtaaaaagc
    37081 gtcaaacgca aggtaacgag gtgaaaaatc atgaatagac aacaaaatga tctaattttg
    37141 caatttgctg cagttattat ctttttcatg gtaatggtct ttggtttttc acttttctta
    37201 gcaggacatt atacacctgg tggcggtttc gtcggcggat tattattcgc aagttcactt
    37261 gttatcatta cgattgcatt tgatatagaa acaatgcgta aaattttccc attagatttt
    37321 aaaatcttga taggtatcgg attagttttt tgtattgcga cgcctatcgc aagttggttt
    37381 ttaggcaaaa atttctttac gcatgtcaca tttgatatac cgttatttat attagaacct
    37441 gttcatatga caacagcagt attctttgat ttcggtgttt tatgtgcagt agttggtact
    37501 gtcatgacaa ttattatttc gattggagag aacgaatagt ggaaattatt atgatttttg
    37561 ttagtggtat tctcacagca attagtgtct atctcgtttt gtctaaaagt ctgatacgaa
    37621 ttgttatggg aactacacta ttaacacatg cagcaaattt atttttaata actatgggcg
    37681 gacttaaaca tggtacggtt ccaatttatg aagcgaacgt aaaaagctat gttgatccta
    37741 tcccgcaagc acttatttta acagcaatcg ttatcgcttt tgcgacaaca gcctttttct
    37801 tagtattagc atttagaaca tataaagaat taggcacaga taacgttgag agtatgaaag
    37861 gagttccaga agatgattga aagtaatatg cttgtgttaa cgctagttat tccagtaatt
    37921 acagccatat tacttgtctt tattggaaag cgtcctatta taaagcgtta tgtcgcacta
    37981 ggcggtacat tattaacctt ggtcgcggca attatcaatt tagcaaatgt tgtaaaacat
    38041 ggaccaatac gcgtagagtt aggttcatgg aaagcaccat acagtattgt ctttgtatta
    38101 gacatattca gtgccctact aattattact agtattatta ttacagctat tgtgatttta
    38161 tattcatacc aaacaatagg tattgaacgt gaaagatatt attattattt ttcagtacta
    38221 tttatgttaa taggtatcat tggtgcattc acaactggtg acattttcaa ccttttcgta
    38281 tttttcgaag tatttttaat gtcatcttat ttcttattag ttataggttc tacaaaaatc
    38341 caattacaag aaacaataaa atatgttctt gttaacgttg tatcgtcatc cttcttcgta
    38401 atgggtgtag cgattttgta ttcagtagta ggaacattga atctagcaga tataagcaat
    38461 aaattagcta acctttcagc gcatgacagt ggcttagtca atatcgtttt catactattt
    38521 atctttgttt ttgctacaaa agctggcgta ttcccaatgt ttgtttggtt acctagtgca
    38581 tactatgcac caccaattcc aatcatagca ttctttggtg ctttgttaac aaaagttggt
    38641 gtttatgcaa tcgctagaac attgagccta ttctttagtg ataatgtaag cttttcacat
    38701 tatgtcattt tattcttagc actattaacg atcatctttg gttgtgtcgg tgctgttgca
    38761 tatgccaaca ttaaaaaaat catcctatac aatgtcatga ttgctgtagg tgtgatctta
    38821 gtcggcgttg caatgatgac agagtcaggt atgattggtg ctatttacta tacattgcac
    38881 gacatgttag taaaacttgc attattcttg cttatcggaa ttatgattaa aattacaggc
    38941 atcgctgatt taagacaatt tggtggctta attaaacgat atcctgtact tggttggtca
    39001 ttctttatcg cagcattaag tttagctggc attcccccac tcagtggctt ttatggaaaa
    39061 tttttcattg tccaatcaac atttgaaaga ggattttatt taagtggtgt catcgtctta
    39121 ttatcaagtt tagtcgtatt atattctgtc attcgcatat tcctacaagg ctttttcggt
    39181 caaccaaaag gatatgatct taataacaaa gtcgacgtca aatatttaac tacgattgca
    39241 atcgttgcag ttgtgataac tgttttatat ggattgtcag cagattactt atatccaatg
    39301 gtaaaagctg gtgcagaaac attctacaat ccatcaacct acgttaaagc tgtattagga
    39361 ggtaaataac atggctgtac aattagtatt aaactttatt atcgcagtgt tttggttgtt
    39421 tgtgacaaat agttacacaa caaataactt tgtgttaggt ttcatctttg gcctagttct
    39481 tgtttacctc ttacacagag tgttaccagg tagattttat gtcatcacgc tatatcgtat
    39541 tattaaatta gttattattt tcttaatcga actgattaaa gctaattttg atgttctgaa
    39601 aattattatt aaaccttcga ttaaaaatga accaggattc tttgtttatc acacagattt
    39661 aaaaaaagat tggcaaattg tcttgttgtc taatttaatt acattaacac ctggtactgt
    39721 agtgcttggt gtaagtgatg atcgtacaaa aatttatatt catgccatag atttttcaac
    39781 taaagagcaa gaagtagaaa gtattaaaac atcattagag aaaattgtaa gagaggtggg
    39841 cgaaatataa tgaatcataa tgttattatc gttattgcat taatcatagt tgtcatttct
    39901 atgttagcta tgctcattcg cgttgtgcta ggcccatcac ttgccgatcg tgttgtcgca
    39961 ttagatgcga ttggtcttca attaatggca gttatagcat tattcagtat tttattaaat
    40021 atcaaataca tgattgtcgt tattatgatg attggtatat tagccttttt aggtactgca
    40081 gtattctcta aatttatgga caaaggtaag gtgattgaac atgatcaaaa tcatactgat
    40141 tagtcttgca cttatctttg ttatcatcgg tgctttaatt agcgccctag cagccatagg
    40201 attattgaga cttgaagatg tatattcacg tgcacatgct gccggaaaag catcaacatt
    40261 aggtgcaatg tcattactat ttggtacgtt tttatatttt attgctacgc aaggttttgt
    40321 aaatatgcaa ttaatcgttg cgattatctt tgtattaatt acaggtcctc tttcaagtca
    40381 tatgattatg aaagcagcat ataatattaa aacgccttat actaaaaaga ctaaagtcga
    40441 tgaaatttcg gaagacttaa aagacacaaa attataactt atataacaaa gtcatgaata
    40501 taaatcaaca acaaacagca gtaagatgat tccaaattag gaatgatttt actgctgttt
    40561 tcttttgaca ttgttacctc tttttcaatg attttagttt agtatatact tctttgacta
    40621 caacttcgcc ctatctatgt atatctcttt aatttaattg cctttcatgt cgttatgtat
    40681 tatgataata ataattataa atcgtaacga ttacgcttta aagagagagg ttttattatg
    40741 cattggacaa ttatcggcgg tggcatacag ggaactgcaa tcgcacaaaa actattatca
    40801 agcggattaa caacagaccg attaacaatc attgacccac acgaaacttt ttgtcaaagg
    40861 tttaactcat atacaaatcg aatagaaatg ccttatttaa gatcaccgat tgtacatcgt
    40921 acatccacaa ccgttccatc taaaacaatt cgctaaacag caccaatata caaatgcttt
    40981 ttatggtcct tatcaacgac ctgaattgac aatgtttatg gatcatattg cacatgcttc
    41041 taaacaatat caattagagg attgcttggt tcaaggttta gttcaaactt tagataaaca
    41101 agaagacaaa tggtatatca agttagaaga tggacaaatt atcactacag attgcgtcgt
    41161 tattgcaata ggcagtacaa atattccgtt tatgcctgac attttaaaag acaaacagaa
    41221 tgtaaatcat atcttcgaga aagaacatga tcaagtagta tatgataaga ccgatcatat
    41281 cgttggtagt ggcattactg cagcacatct tgcacttaaa ttgttaaatc atgatgacga
    41341 taaaaagatt catttatggc taaataaaga tattgaaata catgactttg atgctgatcc
    41401 tggttggtta ggtccgaaaa atatgtcttc atttttaagt actaaaagca tgcctgaaag
    41461 aaatgccatt gtacaacgtg aacgtcataa aggatcaatg cctcacgaac tgtacttacg
    41521 ccttaaaaaa catattaaaa atggtcgtat aaatgtgcat aaaacaccta tcactcaaat
    41581 tagtggtggt gtaattaaca ctgaaaatga ttctgttcca tatcaacaga ttatggtggc
    41641 aactggtttt gaacaagatt ttatgtcaca accacttatt aagcaattaa tacaaaatta
    41701 tgatgcacct atcaacgaat gtaattaccc tgttatttcc gaaaaattag aatggatacc
    41761 aaatctatat gtcgcaggat gctttgcaga cttagaatta ggaccatttg gtagaaatgt
    41821 tatgggtggc cgtaaagctg ccgaacgcat tgaacaagca tttctaaaat tacaacaata
    41881 tagcgcataa aaaaactgct agccgtagtt atgaatatct cataacattt cagctagcag
    41941 ttcttttatt ttaaaggttt aatagcaact gtaccacgca taactgtaat taattgttct
    42001 ttgtcattct taatttttat atcccataca tgtgtcgact taccttgatg aataatttca
    42061 gctgtcgcag taacacgacc atctttagca gaatgaatat gattggcatt catctctaaa
    42121 cctaatggaa taaatttggt tgtatcaatt agattagcag atcctaatga acatgctgtt
    42181 tcacctaaag caatcgaagc accgccatgt aaatatccaa atggttgttt tactttatca
    42241 gtaacaggca tagaaataac aactaaacca tcttcctggt gatctattga catctcaaat
    42301 gtctctaata aatgagtcat attttcacct ctttatgctt atagtaccat agcagcaata
    42361 gtaccgaaaa tcattaaagg aatattataa aacaagaagt ttggtacaca tgtatcgcgt
    42421 atatgatcat gttggccatc aacatttaat cccgcagttg gtcctaatgt tgaatcactt
    42481 gcaggtgaac ctgagtcacc taatgcactc gctgttccaa tcaatgcgat taatgccatt
    42541 gtatctagtc caattgacgc tccaaaagga atgaataatg atgcgataat aggaattgtt
    42601 gcaaatgatg atccaatacc taaagtgaca attaaaccta tcacatacat catgataatg
    42661 ctaaataatt tattatcacc agtaatactt gttaaagttt taactaattc atctatatca
    42721 ccagtagcat tcattacacc agcaaatcca tttgctgtta aaataactac accaatataa
    42781 gccataattt taataccttc aacaaactta gcatctaatt cataccaatt atatgcacgt
    42841 gaaataaaga atacgagtac cccggccagt gcaccaaaaa tcattgaatc tgtatacgtt
    42901 tgtactaaaa atgtagctag tattgctaca attgttacta ttaagatata tggttttaac
    42961 tctgtaacat tgtcactatc tgaaatttta cgtgtttcat attcacgtgg tttacgatat
    43021 acatataaac cgataagcaa gccaacaata taccccattg aaggaataag cattgctttc
    43081 caaatcatat taaactcaat tgggtgattc gcctttgcaa agccactttg aataatttgc
    43141 tggaaaattt gaccgaatcc atatggtaat aacacatacg ggaaacataa accaaaaccg
    43201 ataatcaaac cgattaaacg tctatctatt tttaagtcat taaacagact taataatggt
    43261 gggatgacaa ttggaatgaa tgcaatatgt acaggaatta agttttgact catgatactc
    43321 atagctaata atgcaataat tattgtcact ttcactttaa ctcttgacca tcgactattt
    43381 tcagcgtgaa ttgcatttat aatttttcct actaaatagt ctgtgatacc actgtatgaa
    43441 attaatgctg caaatccacc taataaggca tagcttaatg ctacctcagc accatcgact
    43501 atatttttcc caaatacatt tataactttt tcaatgctca tgcctgaaat taaaccacca
    43561 actagcgcac tgataaataa gcttataact acgtttaatc gacataaaca tagcacaatc
    43621 attaatatta ctgctattac tactgcattt atcatatgac aaccatcctt tatcactcta
    43681 tcgaactaaa gcgtttatag actacatagt atcagtagct ttaacacctg tcaatagcaa
    43741 actataccaa taatctttat tttttagtta attgtctttc ttaaaataat tttcgctttc
    43801 attaaattaa acaattttac aagcctggaa caccaatcaa aatcctaagt tctataatgt
    43861 tatattagta gtcgttgact gaatgaacat atgcttataa taagtttttg caatgctagt
    43921 caagctgatt tatgctcaca aggatatgcg atttatattt tcttacaata atgaaaatgc
    43981 ctgatacaat gcgatccttt atactttcaa gagttaaatt agaaatgtat aacaagacac
    44041 aagttgtatc aggcaattaa ttaaattatt gttgttttaa ccattgtact atcgtcggaa
    44101 tcataaatcc tgttggccct tttggaccat tatatgaagc tttattagtc gttgctggac
    44161 ctgcaatatc gaaatgaatg tgaggtgttt gaccactaaa atgtgttaca aaacttgccg
    44221 caaatagcgc tttaccttgt ccattcgtat ggttaactaa atcagcgata tcactgtgtt
    44281 taatacttgc acgttcggtt gcagtaatcg gtaattcaaa taccatttca tcgactttag
    44341 aacttatttg taatatgtcg tttaatatca ctttactatt tgattcaaat gcagcagctt
    44401 tatcatcgcc aagtgcaacg atggctgcac ccgttaacgt agcaaagtcc ataatcacac
    44461 tcggttgata ttgatttgca taatacacag catctgcaag gactaatcta ccttcagcgt
    44521 ctgtattcat tacttctaca gtttcaccac ttaacgctgt aaatacatca tctggcttca
    44581 ttgatgcttc atttatcata ttttcagcac aagcaagcac tccgacaata tttacaggca
    44641 gttgtaaacg actagccgct tcaatgatac caacgacatt cgcagcgcca cacatgtcaa
    44701 acttcattgt agccatgcca ttcttcgttt taatactata accaccagaa tcatacgtta
    44761 tacctttacc aactaaggca attggtgctt catctttgtc tttgccatta tatgtgatgg
    44821 ttactaatct cggtttatgc ttactacctt tacctaccgc ttgtaaaagt ccaaatcctt
    44881 cagaaactaa agtgtcataa tctttaacct ctactttgac ctttgtattt ttaaaatgat
    44941 taacaatatc ttctgcaaat gtttgtggtg ttaatacatt cggtggcata ttactaaagt
    45001 ctcttgccaa attaatggat tggccaatac tgataccctc atgaatgaaa tctaattcaa
    45061 ttaatgattc actaataagg tttaaattcg tcttaaacag tgccttctta cttgatttat
    45121 aatgatcgaa ttcatatgtt gcacgctcac tttgaatacc gcatgccatt aatacatctg
    45181 ataactgatc atattttgaa ataaatgaat ccataagtaa atacgtatct tcaatgtgtt
    45241 ctgactttat gtattggaaa agatgtcccc atattttcaa catatcttga taactacgtg
    45301 tttttaagtt tcctaaacca actgtaatta aacgatatgt ttgatcttgt acatcaaatg
    45361 cagttgtata aatcttccca actttactac caataatatg ttgatgtttt agtctttcaa
    45421 gtgattctgt aatatcgata tgattaaaac taatgcgttc taactgattt aaatgttctg
    45481 gtataccaat aatcaacgta tttatttcgt tgcttagtgt gttatttaat ttaaaattca
    45541 taatgtacct ccttagattt gatatgttac tcacttcaaa ttgtacaaca ataaagccct
    45601 tcagtgacac tgaaggactt tataaatgaa taatttaatt ttatgtgttt agctatatct
    45661 taatttaaag cctagaattt accttttttg aatgctaaac cgataccacc gattttgaat
    45721 accgcacgtg tatcaatcac ttttttcatg aatgctgctt ttttaccagc gataggttta
    45781 ccaaatacca tacctacacc gtcatgtgaa cctaaagaac aaacagttcc acgatcaacg
    45841 tattcgaatt cttcagttga ttcaccgttt aagatgcgtt taatgttttt agcaacactt
    45901 tcaccttgtt gcattgcaat ttgtgctgta gttggtaatg gacgttcttc tccagctggg
    45961 ataaacgctg aacagtcacc aataacaaaa atgttgtcgt aaccattgat tgttaaatct
    46021 tgctttgtaa cgatacgtcc acgtttaacg ccttcaaatg attcttccat taatttacta
    46081 ccacgtacac cagctgccca tactgaagta cctgcattta attgttgttt ttcaccatct
    46141 acttcaacta cgaaaccttt ttcgttacaa gcaacgattg gtgtagcaat tttaaattca
    46201 acaccgcggt cttctaagta gctaactgcg tggttaacta attcttctga gaacattggt
    46261 aacattttag gtgctgcttc aacacaagtg attttaactt tattttgatc cacaccatat
    46321 ttgctacata attcaggaat tctgtctgtt aattcaccta agaattcaac accagtgaat
    46381 ccagcaccac caactaagat agataaatcg ttatcatctt tttcttttga tgctgcatag
    46441 ttagcaaatt tgtcttcgat atgacgtgat aattcacgtg ctgtgataac attttcaatt
    46501 tggaaagcat gatctttcat accttcgatg ccgaatgttt cactaacgaa acctaatgct
    46561 actactaaaa tatcaaagtc ataaatacct tgatttgttt ctatcttttt agcatcacgg
    46621 tcaatttttg ttacttctgc ttgaacaaag ttcactttgt ctttcttcaa gacactttcc
    46681 acaggatata atacatcttc atagtttagt gtacctgctg atgcttcatg taaccatgtt
    46741 gcttcatagt gatattcatt tttattaata agcgtaattt ctgcttcttc tgttgatatc
    46801 gctttttgca atttagttac agtttgtaaa cctgcataac cagcaccaag tacaagtact
    46861 tttttacgat cttgagccat ttaatttcac ctaagctttc atattttttt aaccaaatgc
    46921 tgataattac tagtaacaac tggtcattac t
//
LOCUS       DAPIDM010000007        44776 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000007 DAPIDM010000000
VERSION     DAPIDM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 44776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.007"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..356)
                     /locus_tag="RJE42_000342"
     CDS             complement(<1..356)
                     /locus_tag="RJE42_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HDV5875796.1"
                     /translation="MKRNFPKLIALSLIFSLSITPIANAESNSNIKAKDKRHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKVT"
     gene            1205..2500
                     /gene="purB"
                     /locus_tag="RJE42_000343"
     CDS             1205..2500
                     /gene="purB"
                     /locus_tag="RJE42_000343"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV5875797.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2609..2911
                     /locus_tag="RJE42_000344"
     CDS             2609..2911
                     /locus_tag="RJE42_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875798.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            3083..3775
                     /locus_tag="RJE42_000345"
     CDS             3083..3775
                     /locus_tag="RJE42_000345"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDV5875799.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTKTQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3772..5964
                     /gene="pcrA"
                     /locus_tag="RJE42_000346"
     CDS             3772..5964
                     /gene="pcrA"
                     /locus_tag="RJE42_000346"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDV5875800.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5968..7971
                     /gene="ligA"
                     /locus_tag="RJE42_000347"
     CDS             5968..7971
                     /gene="ligA"
                     /locus_tag="RJE42_000347"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV5875801.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVSDIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKYKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLTSQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7984..9183
                     /locus_tag="RJE42_000348"
     CDS             7984..9183
                     /locus_tag="RJE42_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDV5875802.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(9272..10810)
                     /gene="putP"
                     /locus_tag="RJE42_000349"
     CDS             complement(9272..10810)
                     /gene="putP"
                     /locus_tag="RJE42_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV5875803.1"
                     /translation="MLTMGTALSRQVDANWQTYIMIAVYFLILIIIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            11177..11479
                     /gene="gatC"
                     /locus_tag="RJE42_000350"
     CDS             11177..11479
                     /gene="gatC"
                     /locus_tag="RJE42_000350"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDV5875804.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            11481..12938
                     /gene="gatA"
                     /locus_tag="RJE42_000351"
     CDS             11481..12938
                     /gene="gatA"
                     /locus_tag="RJE42_000351"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDV5875805.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADANDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12951..14378
                     /gene="gatB"
                     /locus_tag="RJE42_000352"
     CDS             12951..14378
                     /gene="gatB"
                     /locus_tag="RJE42_000352"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDV5875806.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRIKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            14417..14563
                     /locus_tag="RJE42_000353"
     CDS             14417..14563
                     /locus_tag="RJE42_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875807.1"
                     /translation="MVADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            15090..16037
                     /locus_tag="RJE42_000354"
     CDS             15090..16037
                     /locus_tag="RJE42_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV5875808.1"
                     /translation="MRKRARIIYNPTSGKEQFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNAHVDDNLIEE"
     gene            16118..17479
                     /gene="rlmD"
                     /locus_tag="RJE42_000355"
     CDS             16118..17479
                     /gene="rlmD"
                     /locus_tag="RJE42_000355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV5875809.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNKVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMVIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAELTGNEVVLDTYCGTGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLSEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            17649..18179
                     /locus_tag="RJE42_000356"
     CDS             17649..18179
                     /locus_tag="RJE42_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDV5875810.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            18426..19496
                     /gene="dinB"
                     /locus_tag="RJE42_000357"
     CDS             18426..19496
                     /gene="dinB"
                     /locus_tag="RJE42_000357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV5875811.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYRNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSTRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(19563..20117)
                     /locus_tag="RJE42_000358"
     CDS             complement(19563..20117)
                     /locus_tag="RJE42_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDV5875812.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            20334..20426
                     /locus_tag="RJE42_000359"
     CDS             20334..20426
                     /locus_tag="RJE42_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875813.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(20508..21008)
                     /gene="ftnA"
                     /locus_tag="RJE42_000360"
     CDS             complement(20508..21008)
                     /gene="ftnA"
                     /locus_tag="RJE42_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDV5875814.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEEAMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            21300..22613
                     /gene="murT"
                     /locus_tag="RJE42_000361"
     CDS             21300..22613
                     /gene="murT"
                     /locus_tag="RJE42_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDV5875815.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            22615..23346
                     /locus_tag="RJE42_000362"
     CDS             22615..23346
                     /locus_tag="RJE42_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDV5875816.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDRKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEKQAKQVLIDRANRQKKSR"
     gene            23432..23542
                     /locus_tag="RJE42_000363"
     CDS             23432..23542
                     /locus_tag="RJE42_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875817.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMRIYHRIINSNGNYAT"
     gene            23571..23984
                     /locus_tag="RJE42_000364"
     CDS             23571..23984
                     /locus_tag="RJE42_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875818.1"
                     /translation="MNSTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVEFEFALFDSDMDNLKHIEYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(24173..25159)
                     /locus_tag="RJE42_000365"
     CDS             complement(24173..25159)
                     /locus_tag="RJE42_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDV5875819.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDNRYA"
     gene            25743..26501
                     /gene="map"
                     /locus_tag="RJE42_000366"
     CDS             25743..26501
                     /gene="map"
                     /locus_tag="RJE42_000366"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV5875820.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESGDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            26718..27104
                     /locus_tag="RJE42_000367"
     CDS             26718..27104
                     /locus_tag="RJE42_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875821.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAESFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            27119..27820
                     /locus_tag="RJE42_000368"
     CDS             27119..27820
                     /locus_tag="RJE42_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDV5875822.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYIYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            27817..28860
                     /locus_tag="RJE42_000369"
     CDS             27817..28860
                     /locus_tag="RJE42_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDV5875823.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            28850..29479
                     /gene="vraR"
                     /locus_tag="RJE42_000370"
     CDS             28850..29479
                     /gene="vraR"
                     /locus_tag="RJE42_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDV5875824.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(29592..30809)
                     /locus_tag="RJE42_000371"
     CDS             complement(29592..30809)
                     /locus_tag="RJE42_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDV5875825.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDTNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(31087..31362)
                     /locus_tag="RJE42_000372"
     CDS             complement(31087..31362)
                     /locus_tag="RJE42_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDV5875826.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(31369..31833)
                     /locus_tag="RJE42_000373"
     CDS             complement(31369..31833)
                     /locus_tag="RJE42_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDV5875827.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            31980..32180
                     /locus_tag="RJE42_000374"
     CDS             31980..32180
                     /locus_tag="RJE42_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDV5875828.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            32183..33439
                     /locus_tag="RJE42_000375"
     CDS             32183..33439
                     /locus_tag="RJE42_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV5875829.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMNVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            33529..34059
                     /locus_tag="RJE42_000376"
     CDS             33529..34059
                     /locus_tag="RJE42_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV5875830.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(34321..35472)
                     /gene="yfkAB"
                     /locus_tag="RJE42_000377"
     CDS             complement(34321..35472)
                     /gene="yfkAB"
                     /locus_tag="RJE42_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDV5875831.1"
                     /translation="MNSVKLKQPVSIHNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHNRGIYIQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQQQIIQF"
     gene            35696..35857
                     /locus_tag="RJE42_000378"
     CDS             35696..35857
                     /locus_tag="RJE42_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875832.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            35987..36502
                     /locus_tag="RJE42_000379"
     CDS             35987..36502
                     /locus_tag="RJE42_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDV5875833.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            36843..37652
                     /gene="sgtB"
                     /locus_tag="RJE42_000380"
     CDS             36843..37652
                     /gene="sgtB"
                     /locus_tag="RJE42_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDV5875834.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            37913..38731
                     /gene="recX"
                     /locus_tag="RJE42_000381"
     CDS             37913..38731
                     /gene="recX"
                     /locus_tag="RJE42_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV5875835.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTDKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            38709..39023
                     /locus_tag="RJE42_000382"
     CDS             38709..39023
                     /locus_tag="RJE42_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDV5875836.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            39038..40555
                     /locus_tag="RJE42_000383"
     CDS             39038..40555
                     /locus_tag="RJE42_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875837.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAQDIDLNNISLHI
                     YQGEALGIIGEPESSKAMVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVLDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            40564..41400
                     /locus_tag="RJE42_000384"
     CDS             40564..41400
                     /locus_tag="RJE42_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875838.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRLIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMVSALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGICVLCFFVLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDKQQDVSKDSTDEADT
                     SS"
     gene            complement(41661..42638)
                     /locus_tag="RJE42_000385"
     CDS             complement(41661..42638)
                     /locus_tag="RJE42_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV5875839.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVVGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQKLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            42790..43827
                     /gene="mutY"
                     /locus_tag="RJE42_000386"
     CDS             42790..43827
                     /gene="mutY"
                     /locus_tag="RJE42_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV5875840.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARREMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGTINIERLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            44130..44672
                     /locus_tag="RJE42_000387"
     CDS             44130..44672
                     /locus_tag="RJE42_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDV5875841.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT        16371 a         6357 c         7983 g        14065 t
ORIGIN      
        1 gtgacttttg gtgaaattgt aactatataa tcgatattac cttcagtgtt caaaacagga
       61 aaataataat taccatcact ctttttgttg aatttataaa ttttaaaagg ttcaccaagt
      121 gtatacgagg ctttctcttt attatagatt ttatcaagtg aagtaacata agaaagatag
      181 tctttttgcg ctaaattacg tacgtcagtt ggaacactct tgtcctcgac attaacttgc
      241 acatgcctct tatcttttgc tttgatattt gaattgctct cagcgtttgc aataggggta
      301 atacttaaag aaaatattaa acttaaagct attaattttg gaaagtttct tttcatataa
      361 aaactccttt attttattat aaaataatta tattaaattt agttaagaat ttcaatataa
      421 atttttaatc tatgtttaaa aaatatttaa ttattagaca ttatcagttt atgcaataat
      481 ttagtagctt atttactaaa ttgcaaacat ctcattcaat aattaatatg atcgaacgtt
      541 tcatgaaaat atgttagtcg attttgaaag aaacgcagtc aattgatgtc tgtctataaa
      601 aatttactat taagaaaatc tctgagtact tttctataat cactttcgta tttatacgac
      661 tcgaggagtt caatattaat gtcttgtatt taaaatgcca tacctatgca attctttttg
      721 tgagtagaaa atttgttata ttagaagcga ataattttaa aacacattat ggaatgtaag
      781 aaggaatatt atgaaactag gaaaagtaga ttgttgaaat tctatacaat aggagaagta
      841 aagtgaataa tatttgttat tactaaagct attgattgtc ctaattgtaa taaaaagttg
      901 gatagtgtag ttgaggggag aaaaactaaa atgcaataat atattcttaa tggatcatta
      961 taaataccag ttagattaaa gtgttgcgaa catttacaat tagaaatcag tattaatctg
     1021 ccatagataa ttgtattaat gtaaaaaata aaatagaatg agaatatacg gaaatttttt
     1081 atacatttat aaccaattct gttaaactgt gaagagtaga gttaaaaacg aactttttat
     1141 ttatttttat taaaaatgtt ctgtttttga aaattcgagt atacatacga taggagttaa
     1201 aaaaatgatt gaacgttatt ctagagaaga aatgtctaat atttggactg atcaaaatcg
     1261 ctatgaagca tggttagaag tggaaatttt agcatgtgag gcatggagtg aattaggaca
     1321 tataccgaaa gctgacgtac aaaaaattcg tcaaaacgca aaggtaaatg tcgaacgtgc
     1381 acaagaaatt gaacaagaaa cgcgccatga tgttgtagcc tttactagac aagtttctga
     1441 aacgctaggt gaagaacgta agtgggtaca ttatggttta acttctactg atgttgtaga
     1501 tacagcttta agtttcgtta ttaaacaagc aaatgacatt attgaaaaag atttagaaag
     1561 atttatcgac gttttagctg aaaaagcaaa aaattataaa tatacattga tgatgggacg
     1621 tacacatgga gtgcatgcag aaccaactac atttggtgtc aaaatggcat tgtggtacac
     1681 ggaaatgcaa cgtaacttac aacgtttcaa acaagtaaga gaagaaattg aagtagggaa
     1741 aatgagtggt gcagtaggta cttttgctaa cattcctcct gaaatagaaa gttatgtgtg
     1801 taaacattta ggtattggca cagcgcctgt atcaacgcaa acattgcaac gcgatcgaca
     1861 tgcatactat attgcgacat tggcattaat cgcgacgtca ctagaaaaat ttgccgttga
     1921 aattcgtaac cttcaaaaaa cagaaacaag agaagttgaa gaagcatttg caaaaggtca
     1981 aaaaggttca tctgcaatgc ctcataaacg aaatccaatt ggttctgaaa atatcacagg
     2041 tatttcaaga gtcattcgtg gttatattac tacagcttat gagaatgtgc cattatggca
     2101 tgaaagagat atttctcatt cttcagctga gcgtattatg ttaccagatg ttacaatagc
     2161 actagattat gcattgaatc gtttcacgaa tattgtcgac cgtttaactg tatttgaaga
     2221 taatatgcgt aataacatag ataaaacatt tggtctgatt ttctcacaac gtgtcttatt
     2281 agcattaatt aataaaggta tggttcgtga agaagcatac gacaaagtac aaccaaaagc
     2341 gatgatatct tgggaaacga aaacaccttt ccgtgaatta atcgaacaag atgagtctat
     2401 tacaagtgtt ttaacaaaag aagaattgga tgaatgtttc gatcctaaac atcatttgaa
     2461 tcaagttgac actattttcg aacgcgctgg attagcataa aattaaataa agtaacgatt
     2521 cagatttaat aaaatataaa tcgctacact ttaatagatt aatgcgttaa aatgacataa
     2581 agaataaaat tataggggtt gtttcgatat gcaaattgaa aaattacgag gtgcagcgtt
     2641 agatgaattg tttgatgcaa tactaacgtt agaaaataga gaagaatgtt accaattttt
     2701 cgatgatttg tgtactgtaa atgaaattca atcactgtct caaagattac aagttgctaa
     2761 aatgattaag caaggttata cctatgcaac gattgaacaa gaatctggag catcgactgc
     2821 aacgatttct agagtgaagc gttcattaca atggggtaat gatgcttata caatgatttt
     2881 agatcgtatg aatattgaaa caaatgaata ataaatgaac gataaacaat ggttatctat
     2941 ctgcactaat aaggtagata atcattgttt tttcacgaaa aaatttacag agtaaaagaa
     3001 cttaaatttc atattaagtc tttagaactc gacacttaaa aatgctataa tcatatgtat
     3061 gttaaaaaag gagtttcgga aaatgtatga cattaaaaaa tggcgccata tttttaaatt
     3121 agacccagct aaacatattt cagatgatga tttagatgcg atttgtatgt ctcaaacaga
     3181 tgcaattatg attggtggaa ctgatgacgt tactgaagat aatgtcattc atttaatgag
     3241 cagagtaaga agatacccat tgcctttagt acttgaaata tcaaacatcg aaagtgtaat
     3301 gcctggtttt gatttttatt ttgtacctac agtactgaac agtacagatg ttgcatttca
     3361 caatggtaca ttattagaag cgcttaaaac atatggacat agtatagatt ttgaagaagt
     3421 aatatttgaa gggtatgtcg tgtgcaatgc tgatagcaaa gtggcaaaac ataccaaagc
     3481 aaatacagat ttaacaacag aagatttaga agcatatgcc caaatggtca atcatatgta
     3541 tcgattaccg gttatgtata tagagtatag tggcatttat ggcgacgtat caaaggttca
     3601 agctgtctca gaacatctaa caaaaacgca acttttttat ggtggcggta tttcctcaga
     3661 acaacaagcg acagagatgg cagctattgc agatacaatt atcgtcggtg atattattta
     3721 taaagatatt aaaaaagctt taaaaacagt aaaaataaag gagtctagta aatgaatgcg
     3781 ttattaaatc atatgaatac agagcaaagt gaagctgtaa agacaacaga aggaccattg
     3841 ttaattatgg caggtgctgg ttcagggaag acacgtgttt taacacatag aattgcttat
     3901 ttattagacg aaaaagatgt ctcaccatac aatgttttgg ctattacttt tacaaataaa
     3961 gctgcaagag aaatgaaaga acgtgttcaa aaattagtag gtgatcaagc agaagtgatt
     4021 tggatgtcaa cattccactc aatgtgtgtg cgtattttac gtcgtgatgc agatcgaatt
     4081 ggtatagaac gcaattttac gataattgat cctacagacc aaaaatctgt tattaaagac
     4141 gtcttaaaaa atgaaaatat tgatagtaaa aagtttgaac ctcgtatgtt tatcggtgcg
     4201 atcagtaatt tgaaaaatga acttaaaaca cctgcagatg ctcaaaaaga agccacagat
     4261 tatcactcgc aaatggtagc aacggtttat agtggatatc aacgccaatt gtcacgtaat
     4321 gaagcgttag attttgatga ccttattatg acaacgatta acttatttga gcgtgtacca
     4381 gaagttctag aatattatca gaacaaattc caatatattc atgtagatga gtatcaagat
     4441 accaataaag cacaatacac attagttaaa ttattagcaa gtaagtttaa aaacttatgt
     4501 gttgtaggtg actcagatca gtcaatttat ggttggcgtg gtgctgatat tcaaaatatc
     4561 ttatcatttg aaaaagacta cccagaagcg aatacaatct ttttagagca aaattatcgt
     4621 tcgacgaaaa cgattttaaa tgcggctaac gaagtgatta aaaataattc tgaacgtaag
     4681 ccaaaaggac tgtggactgc aaatacgaat ggtgagaaaa ttcattacta tgaagcaatg
     4741 acagaacgtg atgaagcgga atttgtaata cgagaaatta tgaagcatca acgtaatggt
     4801 aagaaatatc aagatatggc aattttatat agaacaaatg cacaatcacg tgtacttgag
     4861 gaaacattca tgaaatctaa tatgccatac acaatggttg gtggacaaaa gttctatgac
     4921 cgtaaagaaa tcaaagattt attaagttat ttgcgtataa ttgccaatag taatgatgac
     4981 attagtttgc aacgtattat taatgttcca aaaagaggtg taggtccttc atctgttgaa
     5041 aaagttcaaa actatgcact tcaaaacaat atcagtatgt ttgatgcact tggagaagct
     5101 gattttatcg gattatcaaa aaaggtgaca caagagtgtc ttaattttta cgagttaatc
     5161 caaagcttga taaaagaaca agaattttta gaaattcatg aaatagttga tgaagtatta
     5221 caaaagtctg gctaccgaga aatgcttgaa cgtgaaaata cactagaatc tcgaagtaga
     5281 ttagaaaaca tcgatgaatt tatgtcagta ccaaaagact atgaggaaaa taccccatta
     5341 gaagaacagt cattaattaa ctttttaacg gatttatcgt tagtagctga tattgatgag
     5401 gcagatactg aaaatggcgt aacactaatg acgatgcact cggctaaggg tcttgaattt
     5461 ccaatcgtct ttataatggg gatggaagaa tcattattcc cacatattag agcgattaag
     5521 agtgaagatg atcatgaaat gcaagaagaa cgtcgtattt gttatgtagc aatcacaagg
     5581 gctgaagaag tgttatatat cactcatgcg acatcaagaa tgttatttgg tcgccctcag
     5641 tcaaatatgc catctagatt tttaaaggaa attccagaat cactattaga aaatcattca
     5701 agtggcaaac gacaaacgat acaacctaag gcaaaacctt ttgctaaacg tggatttagt
     5761 caacgaacaa cgtcaacgaa aaaacaagta tcgtcatctg attggaatgt aggtgacaaa
     5821 gtgatgcata aagcctgggg agaaggcatg gtgagtaatg taaacgagaa aaatggctca
     5881 atcgaactag atattatctt taaatcacaa gggccaaaac gtttgttagc gcaatttgca
     5941 ccaattgaaa aaaaggagga ttaagggatg gctgatttat cgtctcgtgt gaacgagtta
     6001 catgatttat taaatcaata cagttatgaa tactatgtag aggataatcc atctgtacca
     6061 gacagtgaat atgacaaatt acttcatgaa ctgattaaaa tagaagagga gcatcctgag
     6121 tataagactg tagattctcc aacagttaga gttggcggtg aagcccaagc ctctttcaat
     6181 aaagtcaacc atgacacgcc aatgttaagt ttagggaatg catttaatga ggatgatttg
     6241 agaaaattcg accaacgcat acgtgaacaa attggcaacg ttgaatatat gtgcgaatta
     6301 aaaattgatg gcttagcagt atcattgaaa tatgttgatg gatacttcgt tcaaggttta
     6361 acacgtggtg atggaacaac aggtgaagat attaccgaaa atttaaaaac aattcatgcg
     6421 atacctttga aaatgaaaga accattaaat gtagaagttc gtggtgaagc atatatgccg
     6481 agacgttcat ttttacgatt aaatgaagaa aaagaaaaaa atgatgagca gttatttgca
     6541 aatccaagaa acgcagctgc ggggtcatta agacagttag attctaaatt aacggcaaaa
     6601 cgaaagctaa gcgtatttat atatagtgtc aatgatttca ccgatttcaa tgcgcgttcg
     6661 caaagtgaag cattagatga gttagataaa ttaggtttta caacgaataa aaatagagcg
     6721 cgcgtcagcg atatcgatgg cgttttagaa tatattgaaa aatggacaag ccaaagggaa
     6781 tcattgccat acgatatcga tggtattgtg ataaaggtta atgatttaga tcagcaggac
     6841 gaaatgggat tcacacaaaa atcaccaaga tgggccattg cttataaatt cccagctgag
     6901 gaagtggtaa ctaaattatt agatattgaa ttaagtattg gacgtacagg tgtagtcaca
     6961 cctactgcta ttttagaacc agtaaaagta gctggtacaa ctgtatcaag agcatccttg
     7021 cacaatgaag atttaattca tgacagagat attcgaattg gtgatagtgt tgtagtgaaa
     7081 aaagcaggtg acatcatacc tgaagttgta cgtagtattc cagaacgtag acctgaggat
     7141 gctgtcacat atcatatgcc aacccattgt ccaagttgtg gacatgaatt agtacgtatt
     7201 gaaggcgaag tagcacttcg ttgcattaat ccaaaatgcc aagcacaact tgttgaagga
     7261 ttgattcact ttgtatcaag acaagccatg aatattgatg gtttaggcac taaaattatt
     7321 caacagcttt atcaaagcga attaattaaa gatgttgctg atattttcta tttaacagaa
     7381 gaagatttat tacctttaga cagaatgggg cagaaaaaag ttgataattt attagctgcc
     7441 attcaacaag caaaggacaa ctctttagaa aatttattat tcggtctagg tattaggcat
     7501 ttaggtgtta aagcgagcca agtgttagca gaaaaatatg aaacgataga tcgattacta
     7561 acagtaactg aagcggaatt agtagaaatt catgatatag gtgataaagt agcacaatct
     7621 gtagttactt atttagaaaa tgaagatatt cgtgctttaa ttcaaaaatt aaaatataaa
     7681 catgttaata tgatttataa aggtatcaaa acatcagata ttgaaggaca tcctgaattt
     7741 agtggtaaaa cgatagtact gactggtaag ctacatcaaa tgacacgcaa tgaagcatct
     7801 aaatggctta catcacaagg tgctaaagtt acaagtagcg ttactaaaaa tacagatgtc
     7861 gttattgctg gtgaagatgc aggttcaaaa ttaacaaaag cacaaagttt aggtattgaa
     7921 atttggacag agcaacaatt tgtagataag caaaatgaat taaatagtta gaggggtatg
     7981 tcgatgaagc gtacattagt attattgatt acagctatct ttatactcgc tgcttgtggt
     8041 aaccataagg atgaccaggc tggaaaagat aatcaaaaac ataacaatag ttcaaatcaa
     8101 gtaaaagaaa ttgctacgga taaaaatgta caaggtgata actatcgtac attgttacca
     8161 tttaaagaaa gccaggcaag aggactttta caagataaca tggcaaatag ttataatggc
     8221 ggcgactttg aagatggttt attgaactta agtaaagaag tgtttccaac agacaaatat
     8281 ttgtatcaag atggtcaatt tttggacaag aaaacaatta atgcctattt aaatcctaag
     8341 tatacaaaac gtgaaatcga taaaatgtct gaaaaagata aaaaagacaa gaaagcgaat
     8401 gaaaatttag gacttaatcc atcacacgaa ggtgaaacag atcctgaaaa gattgcagaa
     8461 aaatcaccag cctatttatc taacatttta gagcaagatt tttatggtgg tggagataca
     8521 aaaggtaaga atattaaagg tatgacgatt ggtttagcta tgaatagtgt ttattactat
     8581 aaaaaagaaa aagatggacc gacttttagt aaaaaactag atgatagcga agttaaaaag
     8641 caaggtaaac aaatggctag tgagatatta tcaaggttac gtgaaaatga tgatttaaaa
     8701 gatataccaa ttcattttgc aatttataag caatcaagtg aagattcaat cacaccaggt
     8761 gaatttatca ctcaagcgac tgcagaaaaa agtcaaacaa agcttaatga atggcataat
     8821 atcaatgaaa aatcagcttt attaccttct tcaacagcag cagattatga tgaaaattta
     8881 aataataatt tcaagcaatt taatgataat ttgcaatcat atttttctaa tttcacacaa
     8941 gcagtaggaa aagttaaatt tgttgataaa aagccacaac gattagtagt agatttacca
     9001 atcgattact atggacaagc tgaaacaatt ggtattacac agtacgttac tgaacaagcg
     9061 aataaatatt tcgataaaat cgataactat gaaattcgga ttaaagatgg taaccaacca
     9121 cgtgctttaa ttagtaagac aaaagatgac aaagaaccgc aagttcatat ttacagtaat
     9181 taattgtaaa ttaaagataa taaaaaggta ccaatgttag tcataatttt gattaacatt
     9241 agtacctttt taatttgtta agacttatga attatttttc tctaactatg tcgcggactt
     9301 tgtttaagtc agtttcaaca aatgcaccag gttttttagt aagtttactt acaacatatg
     9361 taacgattac acttacaata aatccaggaa taatttcata taagccgaat atttcgttga
     9421 tatgtgccaa tggtttaatc cacgcaatcc aaacgataac gactaaggca cctgaaacca
     9481 ttccacttac agcaccggca cgtgtcaaac ctttccagta aagtgcaaat agcacaagtg
     9541 gactgaacga tgcaccaaat ccggcccaag cattacctac taagtttaaa attgtgtcgt
     9601 ttggattcca tgcaatcgcg atggcaacaa ttgctacaac taatacagat aatcttccaa
     9661 tcataacaaa ttctttttgg tgcgttttag ctttttcttc accacgaatt aatttataaa
     9721 agtcttccgt taatgaacta gatgttacaa gtaattgtga agaaatcgtg ctcataattg
     9781 cagctagaat cgcagcaagt aagaaaccac ctacaagagg atggaagagt acttgactca
     9841 tcacgatgaa taatgtctca ggatcttcta gtttaatatg ataagcaggt acgaatgcaa
     9901 tacctgttaa accaacagcc acagcgccta ataaaccaac agccatccag ctaataccta
     9961 aacgtctagc tttaggtagc atcttgtgtg atttaataga cataaacctt acaatgatat
    10021 gaggttgacc gaaataacct aatccccatg aaaatagaga gataattcct ataaatgata
    10081 accctttaaa tagatttaaa tttgtaggtt tcatagctgc tacatcatga aacgttcccc
    10141 agccgtttaa attcatcata gcaacaatag ggaccataac catcgcaatt aacataatga
    10201 caccttggaa gaaatctgta attgatacag ctaaatatcc accaaagaaa gtatagaaaa
    10261 tgacaatgaa agcaactaat attaaaccga aatgataatc taatccaaaa gcactttcaa
    10321 ataatttacc accagatacg aaaccagaat gtgtatataa tgtaaagaat actacgataa
    10381 tcaatccaga aataatcttt aacacattat ttttatcgtt taaacgattt ttaaagaaat
    10441 ctggtaatgt aattgcatct ccagctaatt cggtataaac acgaagtcta ggagcaacaa
    10501 caaagtaatt tatataagca cctaatgtta aaccgattgt aatccacata gctgatagac
    10561 cagtgctata gacagaacca ggtagaccca taatcatcca tccactcata tctgaagctc
    10621 cagctgataa tgcagtaata tacggtccaa tactacgtcc acctaacatg tactcgctta
    10681 ggttaccagt tgcttgcttg taaccgtaaa agccaataat gattagtatc aaaaagtaga
    10741 cggcaatcat aatataagtt tgccaattgg catctacttg tcgacttaat gctgtcccca
    10801 ttgtaagcat gataaacact ccctttattt aatttataat ttagttactt gaaaacatta
    10861 tacataaaga taaatgttta gaaaagtagg aaaattaaat ttattctttt tgaaatactt
    10921 gtataccaat atagaaaacg ctttcgtatt ttttagatat gagcaaattt ttataattaa
    10981 aattgcataa tcataatatt gaattgggtt gtctaataat aaaagggtgt tgttttcata
    11041 aaaaatgatt tggtaaacga atgataatgt ttatacagtt tatatgaaat taaagtagta
    11101 cctcataaat acttagattt ttaattggaa atttgataca atttagtgat gaatgactta
    11161 aaggaggctt ttattaatga caaaagtaac acgtgaagaa gttgagcata tcgcgaatct
    11221 tgcaagactt caaatttctc ctgaagaaac ggaagaaatg gccaacacat tagaaagcat
    11281 tttagatttt gcaaaacaaa atgatagcgc tgatacagaa ggcgttgaac ctacatatca
    11341 cgttttagat ttacaaaacg ttttacgtga agataaagca attaaaggta ttccgcaaga
    11401 attagctttg aaaaatgcca aagaaacaga agatggacaa tttaaagtgc ctacaatcat
    11461 gaatgaggag gacgcgtaag atgagcattc gctacgaatc ggttgagaat ttattaactt
    11521 taattaaaga caaaaaaatc aaaccatctg atgttgttaa agatatatat gatgcaattg
    11581 aagagactga tccaacaatt aagtcttttc tagcgctgga taaagaaaat gcaattaaaa
    11641 aagcgcaaga attggatgaa ttacaagcaa aagaccaaat ggatggcaaa ttatttggta
    11701 ttccaatggg tataaaagat aacattatta caaacggatt agaaacaaca tgtgcaagta
    11761 aaatgttaga aggttttgtg ccaatttacg aatctactgt aatggaaaaa ctacataatg
    11821 agaatgccgt tttaatcggt aaattaaata tggatgagtt tgcaatgggt ggttcaacag
    11881 aaacatctta tttcaaaaaa acagttaacc catttgacca taaagcagta ccaggtggtt
    11941 catcaggtgg atctgcagca gcagttgcag cgggcttagt accatttagc ttaggttcag
    12001 acactggtgg ttcaattaga caaccggctg catattgtgg cgttgtcggt atgaaaccaa
    12061 catacggtcg tgtatctcga tttggattag ttgcatttgc atcttcatta gaccaaattg
    12121 gtccattgac tcgaaatgta aaagataatg caatcgtatt agaagctatt tctggtgcag
    12181 atgctaatga ctctacaagt gcacctgttg atgatgtaga ctttacatct gaaattggta
    12241 aagatattaa aggattaaaa gttgcattac ctaaagaata cttaggtgaa ggtgtagctg
    12301 atgacgtaaa agaagcagtt caaaacgctg tagaaacttt aaaatcttta ggtgctgtcg
    12361 ttgaggaagt atcattgcca aatactaaat ttggtattcc atcatattac gtgattgcat
    12421 catcagaagc ttcgtcaaac ctttctcgtt ttgacggaat tcgttatggt tatcattcta
    12481 aagaagctca ttcattagaa gaattatata aaatgtcaag atctgaaggt ttcggtaaag
    12541 aagtaaaacg tcgtattttc ttaggtacat ttgcattaag ttcaggttac tacgatgctt
    12601 actataaaaa atctcaaaaa gttagaacat tgattaaaaa tgactttgat aaagtattcg
    12661 aaaattatga tgtagtagtt ggtccaacag cgcctacaac tgcgtttaat ttaggtgaag
    12721 aaattgatga tccattaaca atgtatgcca atgatttatt aacaacacca gtaaacttag
    12781 ctggattacc tggtatttct gttccttgtg gacaatcaaa tggccgacca atcggtttac
    12841 agttcattgg taaaccattc gatgaaaaaa cgttatatcg tgtcgcttat caatatgaaa
    12901 cacaatacaa tttacatgac gtttatgaaa aattataagg agtggaaatc atgcattttg
    12961 aaacagttat aggacttgaa gttcacgtag agttaaaaac ggactcaaaa atgttttctc
    13021 catcaccagc gcattttgga gcagaaccta actcaaatac aaatgttatc gacttagcat
    13081 atccaggtgt cttaccagtt gttaataagc gtgcagtaga ctgggcaatg cgtgctgcaa
    13141 tggcactaaa tatggaaatc gcaacagaat ctaagtttga ccgtaagaac tatttctatc
    13201 cagataatcc aaaggcatat caaatttctc aatttgatca accaattggt gaaaatggat
    13261 atattgatat cgaagtcgac ggtgaaacaa aacgaatcgg tattactcgt cttcacatgg
    13321 aagaagatgc tggtaagtca acacataaag gtgagtattc attagttgac ttgaaccgtc
    13381 aaggtacacc gctaattgaa atcgtatctg aaccagatat tcgttcacct aaagaagcat
    13441 atgcatattt agaaaaatta cgttcaatta ttcaatacac tggtgtatca gacgttaaga
    13501 tggaagaggg atctttacgc tgtgatgcta acatctcttt gcgtccatat ggtcaagaaa
    13561 aatttggtac taaagctgaa ttgaaaaact taaactcatt taactatgta cgtaaaggtt
    13621 tagaatatga agaaaaacgc caagaagaag aattgttaaa tggtggggaa atcggacaag
    13681 aaacacgtcg attcgatgaa tctacaggta aaacaatttt aatgcgtatt aaagaaggtt
    13741 ctgatgatta ccgttacttc ccagagcctg acattgtacc tttatatatt gatgatgctt
    13801 ggaaagagcg tgttcgtcag acaattcctg aattaccaga tgagcgtaaa gctaagtatg
    13861 taaatgaatt aggtttgcct gcatacgatg cacacgtatt aacattgact aaagaaatgt
    13921 cagatttctt tgaatcaaca attgaacacg gtgcagatgt taaattaaca tctaactggt
    13981 taatgggtgg cgtaaacgaa tatttaaata aaaatcaagt agaattatta gatactaaat
    14041 taacaccaga aaatttagca ggtatgatta aacttatcga agacggaaca atgagcagta
    14101 aaattgcgaa gaaagtcttc ccagagttag cagctaaagg tggtaatgct aaacaaatta
    14161 tggaagataa tggcttagtt caaatttctg atgaagcaac acttctaaaa tttgtaaatg
    14221 aggcattaga caataacgaa caatcagttg aagattacaa aaatggtaaa ggcaaagcta
    14281 tgggcttctt agttggtcaa attatgaaag cgtctaaagg tcaagctaat ccacaattag
    14341 taaatcaact attaaaacaa gaattagata aaagataatt agattcatca gactatgatg
    14401 atataacaat gaacccttgg ttgctgactt agatgcaatc gagggtttat ttatatctat
    14461 agaagtcata ttacttttaa ctttattcat tgtacatgtt aatggtaaaa atattaattt
    14521 tattaatgcg ttagctttaa ttatattaag gcaaactgta taataaaaag gtataaaaca
    14581 tttttgtata aagacaacat tatatttaca acatcatttt aaaggtaaaa tagcataact
    14641 gacgaagtct atataacgaa gaacggcaaa aaatgctgaa taaataacaa cctttgtaca
    14701 tattgagata gtatttgttt aagatacaag ttggtcttta acgatattaa gaatgatgaa
    14761 ataagactga gcctgggtca taaattcaat atcctaggca ctacaatgtt acattggcag
    14821 tagttgactg aaagaaaata cgcttgtaac aagctttttt tcaattctag tggggcccca
    14881 acatagaagc tgacttactg tcagcttaca ataatgtgca agttggggtg gggccccaaa
    14941 catagagaat ttcaaaagga aattctacag acaatgtaag ttggggtaga acgaaataaa
    15001 ttttgttaaa tattatttct gtcccactcc ctattagacg aaaccaagat gaagttaaaa
    15061 tatatgaatt ttaagtagag ggataagata tgaggaaacg tgctagaatc atttataacc
    15121 cgacatcagg taaagagcaa tttaaaagag aattacctga tgccttaata aaattagaaa
    15181 aagcgggata tgaaacgagt gcatatgcaa ccgagaaaat aggtgatgcc acacttgaag
    15241 cagaaagagc tatgcatgaa aattatgatg tattaatcgc tgcaggtggt gacggaacat
    15301 taaatgaagt agttaatggt atcgcagaaa agcctaatcg tcctaagcta ggtgtcattc
    15361 ctatgggtac tgttaatgac tttggacgtg cgttgcatat acctaatgac atcatggggg
    15421 cacttgatgt catcattgaa ggtcattcta ctaaagtaga tattggtaaa atgaataatc
    15481 gatactttat taatttagct gcaggcggac aattgacgca agtctcttat gaaacaccga
    15541 gtaaattgaa atctattgtt ggtccatttg cttattacat caaaggtttc gaaatgttac
    15601 ctcaaatgaa agctgtagat ttaagaattg aatatgatgg taatgttttc caaggagaag
    15661 cattattatt ctttttaggt ttaacaaatt caatggcagg atttgaaaaa ttagtgccgg
    15721 acgctaagtt agacgacggc tattttacgt taattatagt tgaaaaatca aaccttgcag
    15781 aacttggtca tattatgact ttagcttcaa gaggagaaca tactaagcat cctaaagtta
    15841 tttatgaaaa agcaaaggca attaatattt catcattcac agacttacaa ctaaatgtag
    15901 atggagaata tggtggtaaa ttaccagcga atttcttgaa tctagaacgt catatagatg
    15961 tctttgcacc aaatgatatt gtaaatgaag agttaataaa taatgctcat gttgatgaca
    16021 atttaatcga agaataaaca gaagtagtga aatttataat ggcaggttag gaagcgcata
    16081 attttgttcc taacctgttc attttggagg taaaattttg caagcaatag ctaaaaatga
    16141 cattaaaacg ggaacagttg ttgatttaac gcatgaaggt catggtgtcg taaaaataga
    16201 tcgctttcct attttcatac cacaagcttt aattaatgaa caaattgaat ataaaattat
    16261 caaagtaaaa aagaactttg cgataggtaa gttgcttaat ataaatacga gaagtgagaa
    16321 tcgtgttgca ccgccatgta tatattatga acgatgtggt gggtgccaac ttcaacactt
    16381 atcatacgaa gcgcaacttg aaatgaaaaa ggaacaagtg attaatttgt ttcagcgaaa
    16441 agcacatttt gataattcta agattaacga tacagttggc atgacagatc cttggagata
    16501 tcgaaataag tcccagattc cagttggtaa gaatgaacag aatgaagtta ttatgggctt
    16561 ttatcgtcaa agaagccatg acattattga catggaaagt tgtttaatac aagattcaca
    16621 acatcaagaa gtgatgaata aagttaaatc tattttaaaa gatttaaatg taagtattta
    16681 tcaagaacaa ttgaaaaaag gtttaatgag acatttggtt gtaagaacag gatatcatac
    16741 agacgaaatg atggttattt ttgtaactaa tggtaaaaag tggccacaaa aaaatgctgt
    16801 tgttgagaag atattagatg catttcctaa tgtgacaagc attaaacaaa acattaatga
    16861 tagccactct aatgttatta tgggacgtca gtctataacg ctatatggta aagatacaat
    16921 tattgatcaa ttaacagata gtacatttaa aataagtgat cagtcattct accaaattaa
    16981 ttctgaacaa acagagaaat tatataataa agcaattgag tatgcggaat tgacaggtaa
    17041 tgaagttgta ttagatacct attgtggtac tggaacaata ggactatata tggcaccaca
    17101 tgcaaaacat gtatacggtg ttgaagttgt accttctgca attgaagacg cacaacaaaa
    17161 tgcgactata aaccaatgca ataatacaac ttttgtctgt gggaaagctg aagaagttat
    17221 tttacaatgg aaagcacaag ggattaaacc agatgttgta atggttgatc cgccaagaaa
    17281 gggctgtgac gaaacgttta ttcagacttt gcttacatta gaacctaagc gtattgtcta
    17341 tatttcatgt aatcccgcaa cacagcaacg tgatgcatta ttattatctg aaaaatatca
    17401 acttgaagag gtaacaccgg tagatatgtt tccacaaaca acacatgttg agacagtggc
    17461 attattcaat ttgaaatagc gcacatgatt tgagataaaa tttcgcctta ataagtgaag
    17521 aaagaagtcg tatattaaaa ttttattaca gccaatttac tttcttgata tgaacttttt
    17581 aaattaaatg tgacattgta tactatattt aaagaagaat aagaatgtca tgattgggag
    17641 ggttggcaat gcataaaatt gatttaacga caaataattt tcaaatgcga aggtttatta
    17701 tcttgcaact tgttattgcg ttattcgtga ttttatttac gtataagtgg gcgttaggtg
    17761 tcacagcggt tgtcgatcaa aatatcatca tcaatcttgt atatgggttt gctggattca
    17821 ttattttatt gattttacat gaacttatcc atagagcatt atttctgtta tttaaaaaag
    17881 atagcaaacc tatgtttaat ataaaaaaag acagaatctt attccaaaca gcagatgctt
    17941 gcttcaacaa atggcaattc tcaattatta tgctgtcacc actaatccta ttaagtactg
    18001 gcttgcttat tttaattaag gtatttggtt attcatcact tatttttatg tttagtatgc
    18061 ataccgctta ttgctttata gatattctgc ttgttgcatt aacaatatct agtagtttta
    18121 aatatgttca acaagatgaa gatagtattt atttgtatca tcaaaagccg actcaataat
    18181 aaattttaat atgatgatat cagcaaaatg aatttgcttg aattatttca gattaccgat
    18241 gttttcttac tcaatcgtct tcatcatcat gcttaaatag caattgattt tataacaact
    18301 tatagttatc tcaatatagt tatcgctaag tgcaagattt atgtaaaaaa atcgtacaca
    18361 gcgtataatt atattgagat atttttgtgc gttataaaag taaaaattag tagggaggtg
    18421 agcacttgac tgagagacga attattcata tagatatgga ttattttttt gcacaagtcg
    18481 aaatgagaga taaccccaaa ttgaaaggga agccagtcat tgttggtggt aaagcaagta
    18541 gtaggggcgt tgtctctacg gcatcttatg aagcacgaaa atacggcgtc cattcagcaa
    18601 tgcctatgtc acaagcgcat aaattatgtc caaatggata ctttgtcaca agtaattttg
    18661 gtgcttaccg agaaacatct gcgcagatta tgtctatttt ccgtagttat acagataagg
    18721 tagaaccgat gtcattagat gaagcgtatt tagatattac agaattagtg agaccagacc
    18781 ttcctgcttc gaaaattgct cagtatatta gaaaagatat tctagagcag acacatttaa
    18841 cagcatctgc aggtgtttct tataacaaat ttttagctaa attagcgagt ggtatgaata
    18901 aacctgatgg tatgactgtg attgattatc gaaatgtcca tgatattttg atgacattgg
    18961 atattggaga ttttccaggc gtaggtaaag cttccaaaaa agtaatgcat gataatggta
    19021 tttttaacgg tagagattta tatgagaaaa cggaatttga attaatacgt ttgtttggaa
    19081 aaagaggtcg gggtttatat aacaaggcac gcggtattga ccatagtgaa gtgaaatcaa
    19141 caagagtaag aaaatcagta gggactgaac gcacatttgc aacagacgtg aatgatgatg
    19201 aagagatttt aagaaaagta tgggagttgt caggtaaaac agctgaacgt ctaaataaat
    19261 tacagaagtc ggctaaaact gtaacggtta aaattaaaac ttatcaattt gaaacgttat
    19321 ctaaacagat gagtttaaga gattctgtta gttctgaaga agatatttat aatattgcat
    19381 atttacttta taacgattta aaagaccctg atgtaccaat tcgacttatt ggtgtcactg
    19441 taggtaattt agaacaatca acttataaaa atatgacgat atatgacttt atataaaata
    19501 aagctccctg caaagtttac atttttacaa tgcttacttt gaagggagta ttttatttag
    19561 tcttagcctt tatcttttag atttttacca taaatattag ttacatatgt taaattttca
    19621 taatttttca gtagcctaaa agtaatcatt gcgcatgctt tggcatcatt caatgcatca
    19681 tgatgaccat gaaaatctaa ttgataaaac tccatcatat gttttaaacc gtatcgatac
    19741 gaatcaacgg ttcttttagc aagttgataa ctacaaaagt aagttaaatt tggagttggt
    19801 aaaccaatat tttgaatgct ttgatgtaag acgttcatat caaatgccgc gttatgtgcg
    19861 acaacaggta aatctgcaat aaattgcatc atatatggaa atacgtagtc aaacgtaggt
    19921 gcattttcaa catcttctgg ttgtatgcca tgaactttaa tattttgttg tgaaaaatag
    19981 tcttgcggat tcacaagagt atgaaatgtt tctgttattt gactatcaat gactttaacc
    20041 attccgacag aacaaatact tgtacgttta ccatttgctg tttcaaaatc aagtgcaaca
    20101 aacgcatctt gtatcatgta gtcaatcctt tcaattaatt aaatgtatat attaataaac
    20161 tttttagtca tttgcttaca aacgatacat aatattatac gtgaaggtat aacgtgaaat
    20221 ctattatttg gatgtaactc tgattaaaaa gtaaatgatg atatgcttta aaatcggtca
    20281 aattaataca acattaaatt tagagttatt aacattagta gtttaaaaaa taaatgacta
    20341 aaaatacaat aattagcctt gaaaatgaaa aaacgcagat caatgattca gaaaatgaat
    20401 ctagtgatct ccgtaaagca aaataatata gacattgggt tattgtaagt gacccaaatg
    20461 tctatcatgt cgtatgatat ttatctgtct attgtagtga tgtttaatta ttcttcgtcg
    20521 aatgtacgag cgccaagttc tttttcgtaa agatataatg cattgctgtc atcgccgata
    20581 cgagttaaat aattgatgtg agtttcaaac attgcttctt cttcgacttg ttcatctaag
    20641 aaccagttta agaatgagat agttgcataa tctttatctt gacgagcgat ttcagataag
    20701 ttatagaaac gtcttgtcac ttcttgttct tgagataagc tgtctttgaa agtttctagt
    20761 atgcttgaaa agtcaatttt tggtgctgaa actgctctga attctgcatg tgcacctctg
    20821 tcgttaatat agttataaat cttttgtcca tggaaacgtt cttctttagc ttgttgaatg
    20881 aagaagtttg caaatccttc gtacgattct ttatcacagt atgctgccat tgccatatat
    20941 gcgtgcgctg caaagtactc atggttcatt tgatcattta atgcttctaa taaattttta
    21001 cttaacattt tgatacacct cttatttgta aattaaaatt actatgaatt aaacttagta
    21061 taacaaactc attctcaatt gtaaataata ataattataa tttaataccc gattttagca
    21121 ctctaattga gaaaatgtat catttagcga gatttaattg agaataaata tcatttttaa
    21181 ttatctaaat gagaatgatt ataatttaga aacgctgtat caatttgtga ttgagaaact
    21241 tttttgaagt tgccttgtca tgttataatg atgcgagtgg aaatttgagg aggaaacaca
    21301 tgagacagtg gacggcaatc catctagcaa aattggcgcg taaagcaagt agagcagtag
    21361 gtaaaagagg aacagattta cctggacaaa ttgctagaaa agtggataca gatatattaa
    21421 gaaaattagc agagcaagtt gatgatattg tatttatcag tggaacaaat ggtaaaacaa
    21481 cgacttcaaa cttaattgga catactttaa aagcaaataa tattcaaatt atacacaata
    21541 atgaaggtgc taatatggct gcaggtataa cttctgcatt catcatgcaa tcaacaccta
    21601 agactaaaat tgcggtaatc gaaattgatg aaggttcgat tccacgtgtg ttaaaagaag
    21661 ttacaccttc aatgatggta tttactaatt tctttagaga tcaaatggat cgcttcggtg
    21721 aaattgatat tatggttaat aacattgcag agacaattag taataaaggc atcaaattat
    21781 tgctaaatgc tgatgatcca tttgtgagtc gtttgaaaat cgcaagtgat acgattgtgt
    21841 actatggtat gaaagcacat gcccatgaat ttgaacaaag tacgatgaat gaaagtagat
    21901 attgtccaaa ctgtggtcgc ttattgcaat acgattatat tcattataat caaattggtc
    21961 attatcactg tcagtgtggt ttcaaacgag agcaagcaaa atatgaaata tcaagttttg
    22021 atgtggcacc gtttttacat ttaaatatta atgatgaaaa atatgatatg aaaatagcag
    22081 gtgactttaa cgcttataac gcgttagcag catatacagt tttaagagag ctagggttaa
    22141 atgaacaagc aattaaaaat ggctttgaaa cgtatacatc agacaatggt cgtatgcagt
    22201 actttaaaaa agaacgaaaa gaagcgatga tcaatttagc taaaaaccct gcaggaatga
    22261 atgcaagttt atcagttggt gaacaattag aaggcgaaaa agtgtatgtt atttcgctaa
    22321 atgataacgc tgcagatggt cgagatactt catggattta tgatgcagat tttgaaaaat
    22381 tatctaagca acaaattgaa gctatcatcg tgacaggtac acgagcagaa gaacttcaat
    22441 tgcgattgaa gttagcagag gttgaagtac caattatagt tgagcgtgat atttataaag
    22501 caacggcaaa gactatggat tataaaggtt tcacagttgc aataccaaac tatacatcat
    22561 tagcgcctat gcttgaacaa ttaaaccgtt cgtttgaagg aggtcaatca taatatgcat
    22621 gaattgacta tttatcattt tatgtcagat aaattgaatt tatacagtga tataggaaat
    22681 attattgctt taagacaacg tgcaaaaaaa cgaaatatta aagttaatgt cgtagaaatc
    22741 aatgaaacag aaggtattac ctttgatgaa tgtgatattt tctttattgg tggtggaagt
    22801 gatagagaac aagcattagc aacaaaagaa ttaagtaaaa ttaagacacc acttaaagaa
    22861 gcgattgaag atggtatgcc gggattaaca atttgtggag gctatcaatt tttaggaaaa
    22921 aaatatatca cgcctgatgg tacagaatta gaagggttag gtattttaga tttttatact
    22981 gaatcaaaga caaaccgatt aacaggagat attgttatag aaagtgatac ttttggaact
    23041 attgtaggtt ttgaaaatca cggtggtaga acatatcata atttcggtac acttggtcat
    23101 gttacttttg gttatggtaa taatgatgaa gatagaaaag aaggcattca ttataaaaat
    23161 ttattaggta cttatttaca cggaccaatt ttacctaaaa attacgaaat cactgattat
    23221 ctgttagaaa aagcttgtga acgtaagggt attccgtttg agcctaaaga aatagataat
    23281 gaagcggaaa agcaagcgaa acaagtatta atagacagag caaatagaca gaagaaatct
    23341 cgttaactct gaacatcaca tcaatggatt taaaattgat aaacgatgaa gtttagtaat
    23401 taatcatata tgtataaaca cacacattat tttggatgga aacaaccaaa ttgatgtgtg
    23461 tttttttgtt ctagtgaata attattatac aatgagaatc tatcatagaa ttatcaatag
    23521 taatggtaat tatgcaacat gaaaaaatga atgatgaaag gaatttaacg atgaattcta
    23581 ctaaagtgat tttaaaagat gcatcttatt tacatagcaa aacatcgata acatttattt
    23641 taaaagatgt agttatcgaa gaagataata aaatttatta tttcgacact agtgcaactt
    23701 tcgaagatca agaagttgaa tttgaatttg cactttttga tagtgacatg gacaatttaa
    23761 aacatattga atacgataat ccagttaccg aaatatattt tatagaacct gatttacatt
    23821 ttacgattat tgattttaat caagaactgc tttgtattta tattgatttt gattctggtt
    23881 taaggcattc aaacatggca acagaatctg gtatttcttt aaggataaat gttgctaaat
    23941 cagattttac taaatttatt aatgaattag cctctttgca ttaatgattt aaatctgata
    24001 cacaattaca atcaaaaaag acagccacat ccctccgtag tttaggcgtg tggctatatt
    24061 tgaatctgaa tatttatgct tgtaatttta aaaagggaga tgctatatac gataaaatta
    24121 cttataaagt aagaatatat ctaatggtgc caattagcat aaggtactta aattatgcat
    24181 atctgttgtc tagcagtcga taaatcatta aaacttcata cagtatatga cttttaattt
    24241 gattttcacc actaatttca agtgctttta ttgtcgaacg taaagtttct acagaatcat
    24301 cttctctctt aaaagaacca tcataaaaaa tatctttgat gctactacta atttttagca
    24361 atgccatttt ttcgtcacct gaaaagttaa cacgagtatt tttaggcaag taaatgatat
    24421 ttgataaatg agtgataaac aaacgattcg tatatgcacg tttagttaat tggttgagta
    24481 atttccaatc acattctttt ttcttatgat agcttaattc atcacgttga taacttatta
    24541 acgtttcaac ttgattgttt aaattgaaaa tatttttata tgctttttcg cttttatcag
    24601 attgcagtct tgataagata agttcttggc agcgattgta aaataattta tacatcaagg
    24661 catctgtctt acttaatttt tcttcgacct gaccataata cttaggtgga aacaccatga
    24721 agttaattaa acctgatgtc acgagtccaa taatcgctgt caatgttcga gacaaaaagt
    24781 tgaatatgta ggcatcatga atacctggaa tcatagctaa agatgttagt acagcaacat
    24841 tcgtaccaac ttgcaatttg agttttgtac agaatagaat cgtgaaagtc gcactcaatg
    24901 catatgtaaa aggtgattga tcaccgaata aatatgtaaa taatactgca aagcctgcac
    24961 caattaccgt agcaggtaat ctacgataac ctttaataag tgatgccttg gcagttggtt
    25021 caattgtgac tacagctgtt aaaatggcat agataggtgt taaatctaat gccatacaaa
    25081 agacagctgt taaaaaaatg gcaataccag ttttaattgt tctggcacca attaaatgtt
    25141 tataccattg atcgttcatt ttttaacctc taatcatcgt aaaatcttag cgagcgcttt
    25201 ataataatag tatcgtacat tggaaaagtt catgtatgta aaacatttga aataagcatg
    25261 cttaagcatt actttgattt tcatatacat taatcaaagt tctgggctcc acaatgttat
    25321 attgttagta gttgactaaa ataaaatgca tttgtaacaa gcatttttca attatagtcc
    25381 ggggccccaa cacagaagct gacgaaaagt cagcttacga taatgtgcag gttggggtgt
    25441 gggccccaac acagagaatt tcgaaaagaa attctacagg caatgcaagt tggcgggccc
    25501 caacatagag aatttcaaaa aagaaattct acaggcaatg caagttggcg gggccccaac
    25561 acagagaatt tcgaaaagaa attctacagg caatgcaagt tggggtgcaa cgataaagaa
    25621 atattttttc tttatcacac tatgtcccac tcactttcca aaatactaaa gtaacatctt
    25681 tagtatatca aagaattttt gctataataa gttataatta tataaaaaag gaacgggata
    25741 aaatgattgt aaaaacagaa gaagaattac aagcgttaaa agaaattgga tacatatgcg
    25801 ctaaagtgcg caatacaatg caagctgcaa ccaaaccagg tatcactacg aaagagcttg
    25861 ataatattgc gaaagagtta tttgaagaat acggtgctat ttcagcgcca attcatgatg
    25921 aaaattttcc tggtcaaacg tgtattagtg ttaatgaaga ggtggcacat gggattccaa
    25981 gtaagcgtgt cattcgtgaa ggagatttag taaatattga tgtatcggca ttgaagaatg
    26041 gctattatgc agatacaggc atttcatttg tcgttggaga atcaggcgat ccaatgaaac
    26101 aaaaagtatg tgacgtagca acgatggcat ttgagaatgc aattgcaaaa gtaaaaccgg
    26161 gtactaagtt aagcaacatt ggtaaagcgg tgcataatac agctagacaa aatgatttga
    26221 aagtcattaa aaacttaaca ggtcatggtg ttggtttatc attacatgaa gcgccagcac
    26281 atgtacttaa ttactttgat ccaaaagaca aaacattatt aactgaaggt atggtattag
    26341 ctattgaacc gtttatctca tcaaatgcat cattcgttac agaaggtaaa aatgaatggg
    26401 cttttgaaac gagcgataaa agttttgttg ctcaaattga gcatacagtt atcgtgacta
    26461 aggatggtcc gattttaacg acaaagattg aagaagaata gttcaacata tactaagact
    26521 aaagtatgaa catcatttag ttccggagcc tattcatatt ggtttcggaa ctgttttata
    26581 ataattaaga acacaatcaa tgcgtcattt caaaaatatg ttgtaataaa gtagtttata
    26641 agcaaacata tcatcgacat caacgaagat acatagcgca tttggtactt taaaacttat
    26701 tataaaaggt gatagttatg aactatgttg aacgttatat tgaacagttt ttgagagcaa
    26761 cagtaagaaa taatatcaag cactaccttt taatgttaga tgaaaaaatg aaaaatttag
    26821 atgattatat gcgttattta attactaaaa aagaacaact tagcaagtta attgatagtc
    26881 taatgctaac attagaaaat aaatatattg atattgctga atcatttcaa attcaatgtg
    26941 caagagaaat caataatcaa gaaattgaaa atattaaatc agagttgaat aaagttgaag
    27001 catattatgc acaaattgaa actcaaattc aacaaacttc aactgaaaaa atagcaacag
    27061 aaaaaacatc gtatctaata aattatatga acgctgtggc atagaaaggc ggcgaaacat
    27121 gacacacaaa tatatatcaa cgcaaatgtt gatcattttt actgcattaa tgattattgc
    27181 caatttttac tacatatttt ttgaaaaaat tggcttttta ctcgttctat tattgggatg
    27241 tgtattagtt tatgtaggat atctttattt tcataaaata cgtggccttt tggcgttttg
    27301 gataggcgcg ctattaattg cattcacatt attgtctaat aagtatacaa tcatcatctt
    27361 gttcgtcttt ttattactac ttattgtgcg ttatttaata cacaaattta aaccaaaaaa
    27421 agtagttgcg acggatgagg ttatgacttc accatctttt attaaacaaa agtggtttgg
    27481 tgagcaacga acaccagttt atatatataa gtgggaagat gtccaaattc aacatggaat
    27541 tggcgaccta catattgact taacaaaagc tgcaaatatt aaggaaaata ataccattgt
    27601 tgttagacac attttaggta aagtgcaggt tatattgccg gttaattaca atattaattt
    27661 acatgtagct gctttttatg gaagtactta cgtgaatgaa aaatcatata aagttgaaaa
    27721 taacaatatt catattgaag aaatgatgaa accggataac tatacagtta atatctacgt
    27781 atcaacgttt atcggagacg tagaggtgat ttatcgatga accactacat tagaacaatt
    27841 ggttcaatgc tcatcttagt atatagcatg ctagctgcat ttctgttcat cgataaagtt
    27901 tttgtaaata tcatctattt tcaaggtatg ttttatacac aaatattcgg aataccagtc
    27961 tttttatttt taaatctcat catcatatta ttgtgtatta ttgttggttc ggttctcgct
    28021 tacaaaatca atcagcaaaa tgattggatt aagacgcaaa ttgagcgttc aatggaaggc
    28081 gaaacagttg gcattaatga tcaaaatata gaaatatata gtgaaacgtt agatttatac
    28141 catacactcg tacctttaaa tcaagaattg cataaattgc gacttaaaac tcaaaactta
    28201 accaatgaaa attataatat taatgatgtg aaagttaaaa agattattga agatgaacgt
    28261 caaagactag cacgagaact tcacgattct gttagtcagc aactttttgc ggcaagtatg
    28321 atgctatctg ctatcaaaga aacgaaatta gaaccaccat tagaccaaca aattccgatt
    28381 ttagagaaaa tggttcaaga ttcgcagcta gaaatgcgtg ctttgctgtt acatttaaga
    28441 ccgcttggtt taaaagacaa atctttaggt gagggtatta aagatttagt tattgattta
    28501 caaaaaaaag tgccaatgaa agttgtgcat gaaatacaag attttaaagt gcctaaaggt
    28561 attgaagatc atttgttcag aattacacag gaagcaattt cgaatacatt gcgtcattca
    28621 aacggtacaa aagtgacagt agaattgttt aataaagacg attatttatt gttgagaatt
    28681 caagacaatg gtaaaggttt taatgttgat gaaaaattag aacaaagtta tggacttaaa
    28741 aatatgcgtg aaagagcttt ggaaattggt gcaacgttcc atattgtatc attgccagat
    28801 tcaggtacac gtatcgaggt gaaagcacct ttaaataagg aggattcgta tgacgattaa
    28861 agtattattt gtggatgatc atgaaatggt acgtatagga atttcaagtt atctatcaac
    28921 gcaaagtgat attgaagtag ttggtgaagg cgcttctggt aaagaagcaa ttgccaaagc
    28981 ccatgagttg aagccagatt taatattaat ggatttactt atggaagaca tggatggagt
    29041 agaagcgacg actcagatta aaaaagattt accgcaaatt aaagtattaa tgttaactag
    29101 ttttattgaa gataaagagg tatatcgtgc attagatgca ggtgtcgata gttacatttt
    29161 aaaaacaact agtgcaaaag atatcgccga tgcagttcgt aaaacttcta gaggagaatc
    29221 tgtttttgaa ccggaagttt tagtgaaaat gcgtaaccgt atgaaaaagc gcgcagagtt
    29281 atatgaaatg cttacagaac gagaaatgga aatattatta ttgattgcga aaggttactc
    29341 aaatcaagaa attgctagtg catcgcatat tactattaaa acggttaaga cacatgtgag
    29401 taacatttta agtaagttag aagtgcaaga tagaacacaa gctgttatct atgcattcca
    29461 acataattta attcaatagt tcatttcgaa ttaagaaaag ttacttacgc cgatcacaat
    29521 ataacatcaa atatacacta aaaaacagag gtcttagcaa tcattagcga aaacctctgt
    29581 tttgttgtta tttagtcttt tttaagctta tcaacgattt tgtcttttat atttttatca
    29641 tcttctggat aggtatcact aggatgttcg tcaacatgat acgtacgttg atgtgttgca
    29701 ttagtatctt catcatattc gtacctagtg ttctcattat aatgattttt attattgtca
    29761 tcatgttcta atgctgcttc ttcaggtgtt ttacctttaa ttacactacg ctgatgaatg
    29821 attgcattga tttcagcacc gacaattata ataaaacttg tgatatataa ccatagtaac
    29881 aaaatgatga tacccgcgat actgccatat gttttagaat agttaccaaa atttgaaata
    29941 taccaaccaa aaccaaatga accagctaac caaataattg aagtaaatac tgcacctggt
    30001 aatactgact taagtttcgt tttaacgtta ggtgcaaccg aatataacac gataaatacg
    30061 ataaatataa taatgattgg taacacaatt ctaataagat taaaaatcca tttcacttgt
    30121 tcgtcaaatc caagtggacc gaataggaaa tgactaatta cagaaccaag cgttggtaat
    30181 gctagagcaa ctacaaacac aacgcccata actacagtga agacaacact tagtagtttt
    30241 aatacgattc cattacggct atcttctaca tcgtaagcaa cattgaaaga attcataatt
    30301 gcagtcattc catttgaagc tgaccaaatt gccaaaatca aaccgataga taataagcca
    30361 ccactggagt tttgagttat atcaccaatt acactcttaa ttagagtaga ggtttcagcg
    30421 ggtgcattac ttaacatatt agtaatttga ctctgcttaa tattgaaaaa tggtaataat
    30481 gttaatagga aaagcaacat agggaacatt gcaagtacga aatggtaagt catttgcgct
    30541 gctagtcctg aggcatcatc ttttcctatg cgatagatga gatatgataa gaaattagag
    30601 tctttcttat attttgcagg tttattcaat cttgataaga agaaaacctg atcatctttt
    30661 ttaggtttct tagattggaa ttgttgaggt tcaatatatg tacgatcaac atcaattttg
    30721 ggattacttt tatttttctt ttttgactcc tcttgcgctt ccttaacaga attaaggtat
    30781 tttgaagaag agtggtcctt tttcgacatg tgcactcctt aacctttctc tatgtaatgt
    30841 tttataaagt aactattagt atttatccaa ttataaaaaa actaaacaat gatatgcttt
    30901 aaaaatgata atattataga aattaattga tttaaatctt tcataggcta ttttgattta
    30961 aaattaaaac aaaggcaata ctgaaacaat gcagtttggc caatcattgt attatcattt
    31021 aataatgata actacgaaag gtgtacacca aattgttaag tattacctta taaaaacaaa
    31081 atcatcttag cgttgatttt ttctattaac aaatgtttct ttcgcatcct ttaatgaacg
    31141 ttctaattca ggattattac gacgaatttc ttcaacaaca tctttccagt ataaaacttc
    31201 gtctttaatc ttgctgactt tagaaggctt acgagtgcgg ttaccatttt ttgcatcttt
    31261 aactgattga actaaagctt gacgtgtaga tttatcagct aaactaattg caccaccaat
    31321 tacggcacca attaaaatac caggaacaaa tttattttcc ataaaaaact acccctcttt
    31381 caaatttgca tcttttacga tgtagtctat taaattatca caagatgata ataccatgtc
    31441 gtatacacct tcaaaattat ttgtgtagta tggatctggt acatcactct cttccatatt
    31501 actaaattct aacagtttga acaattgtcc cttaagatta ggattgatag atttaatatt
    31561 atcaacgtta ctttgatcca tagccacaat gtaatcaaaa tcatctgtcg cttcgaataa
    31621 ttcactaatc atgccatcaa atggaatatt gtgtttgttg agaatttttt gtgtaccttc
    31681 atgaggcggc tctcctaaat tccagctacc agtacctctt gaatgtactt ttatatcatg
    31741 aatatttctg tctttaagtc tttgtcgcat gattgcttct gccattggag aacgacatat
    31801 attgccaaga cagacaaatg ctacatctac cattttgatt cctccaaact atgtagttat
    31861 atccccattt tatagcgact ttaaacaata agaaagcaga ttatataaaa atctattaaa
    31921 gtttattaaa ttgtgatact ttgataacat aactattatt agaggtgaac attgtggcta
    31981 tgacaaatga agagaaagta ttagctatta gagagaagtt aaatattgtt aatcaaggat
    32041 tattagatcc tgaaaaatat aaaaatgcaa atgaagaaga attaacagat atatatgatt
    32101 ttgttcaatc aagagaaaga ttgtcgccaa gtgaagtgac agctattgct gacgctttag
    32161 gacaattgcg acacgactag gagtgggaat tttgactaat tacaaagaaa agttacaaca
    32221 atacgctgaa ctattagtta aggttggtat gaatgttcaa ccaaaacagc cagtatttat
    32281 tagatcatca gtagaaacat tagaattaac gcatttaatt gtagaagaag catatcattg
    32341 tggcgcttca gatgtacgtg ttgtatatag cgatccaacc ttaaaacgct taaaatttga
    32401 aaatgaatct gtggaacatt ttgctaatca tgagataaaa tcatatgatg ttgaagcgag
    32461 aatggattac gttaaacgtg gcgctgctaa tttagcactt attagtgaag atcctgattt
    32521 aatggatggt atcgatagcc aaaaattaca agcctttcaa caacaaaacg cacgcgcatt
    32581 taaaggatat atggaaagcg tacaaaaaaa tcaatttcca tgggtcgtag ctgcttttcc
    32641 atctaaagct tgggcgaaga gagtatatcc tgaactatct gtagaagaag catatattaa
    32701 gtttattgac gaggtatttg atattgttcg aatagatggt aatgatccag ttgaaaattg
    32761 gcgtcaacac atcgctaatt taagtgttta cgctcaaaag ttacagcaaa aaaattacca
    32821 tgcgttgcat tatgtatctg aaggtactga tttaacagta ggtttagcga aaaatcatat
    32881 ctgggaagat gctacaagtt atgttaacgg caaggaacaa gcttttattg ctaatatacc
    32941 aactgaagaa gtatttacag caccagatcg aaatagagta gatggatatg ttactaataa
    33001 attaccttta agttataatg gcacgattat cgatcaattt aaattgatgt ttaaagatgg
    33061 agaaattatt gatttttcag ctgaaaaagg tgaagcggta ttgaaagatt taatcaatac
    33121 cgatgaaggt tcaagaagat taggtgaagt agcgttagta cctgatgatt caccgatatc
    33181 aaatcgtaat accatttttt acaatacttt atttgatgaa aatgcagcat gtcatttagc
    33241 gattggatct gcttatgcct ttaatattca aggtggaacg gaaatgaatg ttgaggaaaa
    33301 gattgcaagc ggattaaatg attcaaacgt acatgtcgac ttcatgattg gtagtagtga
    33361 tttgactatt tatggcatat tcgaagatgg ttcaaaagaa ctagtatttg aaaatggaaa
    33421 ttgggcatca acattttaat aaatgttatt ttgaggtgct gagtaggaaa tgaaacatgt
    33481 atttaaaggt caaatacgtg attaaagtat agattgggag ataaaataat gacaaatcag
    33541 gacagaccaa tgaaatctat gtcagaatca aaatgttata aaaatagaca agttttccct
    33601 caagatacga atcaccatca tacaatgttt ggtggtacat tgatggctaa tattgatgaa
    33661 attgcagcaa tcacagctat gaaacatgct ggtgcgcaag tagttaccgc atctacagac
    33721 tcagtagatt tcttaaagcc gattaaaaca ggggacatat tacaatacgt agcgatggtt
    33781 tcatacgctg ggacaagttc gatggaagtg gtcgttcaaa ttagaattga cgatgtattt
    33841 aataacaagc atgatttagc tgcattaagt tacttaacat ttgttgcgtt agatgatgaa
    33901 gggaagccta aacatgtacc tggagtatat ccagaagatg atgttgaaaa atggttttat
    33961 gatacagcac cacaacgtgt tgaacgaaga aaagcacgtc gcattgaaag taaacaaaca
    34021 attgaatatt tagctcaagt gcaacatatt agagactaaa aatataaaga cagattgtta
    34081 agtatgaatt gaactgttat gttcgaatat tccacataca atctgtcttt ttgattatgc
    34141 acatttatgt ttttaggtaa attttaccct tttattatag actactatta gttctattaa
    34201 atgaccttta ttatttatgt agagtgctgc cagcaattaa ttgtttttta ttttgtaata
    34261 gcattaaaat atgcggagac tacttatcga taaagtgctt ctccgcataa ttaagcgttt
    34321 ttaaaattgt ataatttgtt gttgtttgtg catatgacgc tcattatctt taaaatccat
    34381 attcggatag tacatatttt taacaagaac atttggtcct aaacaactaa actcggaaca
    34441 atgacaattt aatgatttag caagatcaga ggataaccat ttatcaaata catctgttaa
    34501 tttatctttt tgtatattcg aaattgtacc tgtttcatct ccgaaatcag ttacgattac
    34561 attacctgta aatacattga catttaaacg actacggcca tccgggtcat ttctagttgt
    34621 tacattttta gcagttctta aacgtgatag taacttttga tcttcatcat cttttaagca
    34681 tggaaacaca ggcaaagtac caaataacat ccaaatatct tcatctctga aatccaatat
    34741 atcatgaatt gtctttttca tttccgctag agttaacaca tttaattgac ttgcaaagtc
    34801 agctggatac atagggtgaa tctcatgtct gctacatttc atatcatgaa cgacttcttg
    34861 atgtattttt cgtaaatgtg gtagcgtact ttgattgagc attgtttccg cagatacaaa
    34921 cattccttgt tctgataatg tacgtgcatt agaaatcatt tgttcatata attttaactt
    34981 agcttttaac ggtggttgct tcttcattgc gccaaagcca acatttgcga attcatcagt
    35041 tgttccccag ttatgtgaga tatgcataac atcgatatat tcagcaatat ctaaataacg
    35101 atcttgaggc aatgttaggt ttgaattcat ttgtatatat atacctctat tatgtgcata
    35161 ctttaataga ggtttaacaa catttctaat agacttttta gaaaacattg gttcgccacc
    35221 tgtaattgac atcgttcgca gatttggaat ttcatcaagt ctacgataaa ttaagtccat
    35281 atctaaaggc tcggggtcga cagtttgtaa agtataacca actgcacagt ggctacaacg
    35341 catattacaa agatttgtag ttgtaaattc gatgttactt aaagttaatt ggccatgttc
    35401 tttaacatcg ttatatgctt cccatggatc attgtgaatg ctaacaggtt gcttcaattt
    35461 gacgctattc atcgtcttaa acttccttct ttctatttgg ttcatatcgg tatgatacca
    35521 cttcgtatta tcactcgtaa caaattatct cttaatttta gttaattgaa aaggggattt
    35581 ttaaaagtca aaataatttg aatgtcataa atatagctaa gattatatcg ctttaacgca
    35641 atttctgtta ggataataat gaaaaagaaa acaaaataaa aggagttaac tttttatgaa
    35701 tgaacagcaa acaatcgaac agataaaagc gcgtttaaat aagtttattg aggatatcga
    35761 tcatgtaaat cctgatgaag tacgtgttga agatatagat gaatggattg gattgttaga
    35821 tcagctcgaa gaaaaggtta aattagtatc taagtaaatg ccacataaga tgtgttagag
    35881 ttagatatcg cgacagtcgc ttaatatttt tatatcttgt atgtttaatc attgatttac
    35941 ttgtctatag tatttgtatc aatgatatat agaaaggtga ttttcaatga ctaaaaaagt
    36001 agcaattatt ctagcaaacg aatttgaaga tatagaatat tcaagtccta aagaggcatt
    36061 agagaatgct ggctttaata ctgtagtgat tggagatact gcaaatagtg aagttgttgg
    36121 taaacacggt gaaaaagtta ctgtcgatgt aggcattgca gaagctaaac cagaagatta
    36181 tgatgcatta ttaattcctg gaggattttc accagatcat ttacgtggag atacagaagg
    36241 tcgatatggc acatttgcta aatactttac taaaaatgat gtaccaacat ttgccatttg
    36301 tcatgggcca caaatactaa tagatacaga cgatttaaaa ggtcgtacgt taacagcagt
    36361 attaaatgta cgcaaagatt tatcaaatgc aggcgcacat gtagttgatg agtcagtagt
    36421 tgtggacaac aatattgtaa caagtcgtgt accagacgat ttagatgatt ttaatcgaga
    36481 aattgttaaa caattacaat agaagtattg tggttatcga ttgttaaaat ttatatattt
    36541 tcatttgaaa tgaaatatag atttcagttt taaaaaatga ataacaaata aaaggtcatc
    36601 tctattggca tttagtaggg atggtctttt atttttataa aatataacaa tatttgacat
    36661 gattaatatt taaaatcaca atgatttatc tataatcttt tcaaaaaaca aaaaagtaag
    36721 tatgatggcg tatacgattg taagcccttg gactgatttt cccaaaaagt gtagcctatt
    36781 tgtgatagta ctggtaaact caaggtatat actaagtgag ttttaaaaga aggagcaaac
    36841 gcatgaaaag aagcgatagg tactcaaact caaatgaaca ttttgagcat atgaaacacg
    36901 aacctcacta taatacgtat tatcaaccag ttggcaaacc gccgaaaaag aaaaaaagca
    36961 aacgaatact attgaaaata ttattaacca ttctaatcat tatcgcattg tttattggta
    37021 tcatgtattt tttatctaca cgcgataatg tggatgaact aagaaaaatt gaaaataaaa
    37081 gtagttttgt gtcagctgat aacatgccag agtatgttaa aggtgccttt atttcaatgg
    37141 aagatgaacg attctacaat catcatggat tcgatttgaa aggtacaact agagctttat
    37201 tttcaacgat tagcgacaga gatgtgcaag gtggtagtac cattacacaa caagttgtca
    37261 aaaattattt ttatgataat gatcgttcat ttactagaaa agtaaaagaa ttatttgtag
    37321 ctcatcgagt tgaaaaacaa tataataaga acgaaatttt aagcttttat ttaaataata
    37381 tttactttgg ggataatcaa tatacgcttg agggcgcagc aaaccattac tttggaacaa
    37441 ccgtgaataa aaatagtaca acaatgtctc acattacagt tttacaaagt gctattttag
    37501 ctagtaaagt caatgcacct agcgtatata atatcaataa tatgtctgag aattttacgc
    37561 aacgtgtaag cacgaactta gaaaaaatga agcaacaaaa ttatatcaat gaaacacaat
    37621 atcaacaggc tatgtcacaa ttaaatcgtt aaatacttgc atttatgtgt aagatacttg
    37681 gaacaataat gtagaaggtt taattgaaat gtcataatga taaagttgaa ttgtttctgc
    37741 ttaataaatt ttacagattt gcttgtaatc acactttcat tttaaaagat gatagtgtga
    37801 ttttttgtta gcatcgaaaa atatttaaag ttaaacattt gctataggaa gtggcttatt
    37861 gaataataaa agagtattgt attaaaactt gagaaagtaa cggggtgatc caatgccgaa
    37921 gattactaaa atagaagttc aaaagaaaaa caaagaacgt tttaatcttt ttttagacga
    37981 acaatttgaa atgggtatag atatcgatac attagtcaaa tttaatttaa aaaaagggca
    38041 acaacttgaa gctgctgaca tggcagagat tcaaaagtat gatcattatc gcataggttt
    38101 aaataaagca atccaatatt tatcatataa aaagagaact gataaagaag ttatacaata
    38161 tttacaaaaa gaagagatat cagaacaagc gatttctgaa gtgattgaat attgttatcg
    38221 cgaaaagtta atcgaccatc aagattatgc ggaaagttta aaaaatacaa tgattcgcac
    38281 gacagataaa ggacctaaaa tttatcaaca aaaactatat caacttggta tagaaccaaa
    38341 tatcattgaa atgtttacag aactttatag agaacaacag gaacttgatg atattatcca
    38401 aattgctgaa aaaatatcta aaacaaaaaa agggccacaa aataaggtta aagaaaaagt
    38461 aatgcaatct ttaattcaaa aagggtttga aatggaaacc attcatgctg tattgaatga
    38521 aatggatttt acacaagatg aagcggtttt agacgattta ttacaacgag atttagaaaa
    38581 aatttataat aaaaatcgaa agaagtacac gcaacaaaaa ttaatttcaa aaactatcga
    38641 aggccttatg agaaaaggat ataaatatga taaaattaaa gctaaattag aagaaagtgg
    38701 tattgccgat ggaacagaag aaattgagtg agatgtctga gccagaatta agacacgaaa
    38761 tacaacttta taaagagaaa atgagaaaag ctgagatgaa tggtatttta aatgaatatg
    38821 acgtttatca aagtaaagta attgttgccg aaagttactt agtagacaga aaaaagattg
    38881 aaattggtaa aatttataaa ctgactgatg gcagtaatca atattttaaa gtagaaagat
    38941 taaaaggtat ttttgcgtgg gggtttagat ttaatagcga tgaacccgaa gaaggcttac
    39001 ctatagcgtt attacaatta tagaaggatg aaaatagatg ggaagttcaa ttgttttaaa
    39061 gttactaaaa gtaacacact actataggaa taaacagaat aaaaaatggt atttaccttt
    39121 tggatatgat gctcaagata tcgatttaaa caatattagt ttacatattt atcaaggtga
    39181 agcattaggt ataattggtg aacctgaatc ttccaaagcg atggtaggtc aattgttggc
    39241 aggtgcaatt aaacccgata aaggtaaagt agtttgtact gaagatttgt tctacggata
    39301 tattgaagat caatcgttaa ttcatcaaac tgttgaagct tatacagcgc agttagttca
    39361 actatttcca tatgaaatta atgatcataa agctgaacag attattcaat atgcacattt
    39421 aggcgattat aaaacgaagc cggttaacca tatttcgaaa gcggcatacg ctcaattact
    39481 attaagtatt gcacgctcat caaaatcaaa tattattatt ttaaatcatg ttattgacta
    39541 tttaacacca caatttatgg aacgtgcgat tgaattaaca aatgattata ttgaaaataa
    39601 tttaacgatt gtgtcaattg gtgatgatat tgataaaatt tcacaagtga gtaactacat
    39661 agcttggttt tcacatggtc aattaagaat ggaagggtca cttaaacaag ttattccatc
    39721 ttttaaagaa catgaacgtg atcgattaag tttaaactca aaagaagaaa ttgaaaactt
    39781 tgatttagat tggaaaaaga atcgtacaag aataccggag atgacctata attttaaacg
    39841 cgttgagcgc tataatcatg caaaaccgcc taagttttta gtgcgttttt ggactttagc
    39901 ctcaggtact attttaggct tagcattgat gatgttgctc attttcaata atataggaat
    39961 tatttcgata acagatttta cgaatcgtgc tacgatgcaa aatgaaaata aagatccata
    40021 tggcgaaaag ttagcttatg gaattgcttt taatggcagt gtggatatgc aaggggataa
    40081 acaagtcaca attccaaaat atagtgtagt tacaattact ggcgaaaata gtaaaaatta
    40141 tcgtgttacc gctgataata agacttacta tgttagtaaa gataaattag aatattttaa
    40201 cccggcaggt ttatatcaaa cgcatagttt taaaaaatta gcgccatata tgaaatcaaa
    40261 ttatagtaat tactatgcat actttaacag tcaattacat aaaaagcata gttcagttat
    40321 taaaacttta gttcttgatg atgataaccg tttcgttgca tccgttacac aacaaccgat
    40381 acaattactt ttcaatgata ataatcagtt atacggtttt gtttatccaa tcgtagataa
    40441 aaaagaatta aaagataagt ttaatattaa caataacatt tggattacta aagttgggaa
    40501 tggatattgt attgccaatt taaaagaaga caaatggatt tatattgaat tgtaggtgta
    40561 aagatgctag ataatattat tttatatttt aaaaacttac cgcatactaa aaggtatgta
    40621 acagaacgtt taaaacaaag ttggaaaagt tttctaatcg tattagctgc atgtttaata
    40681 cttattattg caagtgaaac gctattttcc tttagtcatt tgaccgatgt taaagaggtg
    40741 cgatggttat ttagacttat tgcattgatt gtttttgcgg tattgatgtt cacaatttat
    40801 atctcttatc atcattatat gaatgacttt ttagttacta agttatttaa tatttccgca
    40861 gcgacgccag tagttattat gtctatttta agttttatta tgctagtcat tttgacgatg
    40921 gttagtgcat tggttaaacc agtaaacttc gaaacatcat atatagcgct attctatttt
    40981 attgtaatgg caactatttt tgtaggatta atatcagtta cttttggtct tattcgactg
    41041 ttaactgaaa aaataaatat catattttat gggatttgtg tattgtgttt ctttgtgtta
    41101 ccaattattt tcataccaaa tccaaatcat gtatttatca atcacatcct aatgctgaat
    41161 ccaatgtatt acattgttaa tggtatagca caatctatca tatttggtat atcaagtatg
    41221 gaaaacattc catatcattt ttactttatt ttattcttat gtttaatagc tgcagtaaat
    41281 ttcgtattag caaggtatac gacacacgca atttataata aaacatctaa agtgacacaa
    41341 actgataaac aacaggatgt ttcaaaagat agtacagacg aggcggatac ttcttcatga
    41401 tgtttagctg acctgttgtg taaattaatc tgtgagaatc agaaacaatt agaatataat
    41461 ctagatattg ttttcctata ctgattttga tatttcgagt cattaaaggt agtttgttag
    41521 cgacaatata aatattaggt tttgtaaaag cgttcatagt tttacattaa aaaaccggac
    41581 agagtaaaaa tcgttttgat tttcattctg cccggttgtt cattttttat agattaactt
    41641 tagtgaataa taacttgaaa ttatttagca tacagtttac gttgtaactt atcttctgta
    41701 aatacccagc caatataaga gtggtctatg acattatcgt ttgttacatg tgcaattgcc
    41761 acaaatgaat aatgatcatt ttttaaataa cgcaaatcaa tcaaacgaat ttcagtagaa
    41821 ccatctgcta acgttgttgt ttgccagcga tagattgaag aaaagtttaa aaaagtacgt
    41881 atatctttat tacctttgac tttccataaa atggaatctg ttgataattt ttgacgttcc
    41941 actttatcac taaacaccac attcctacca tatgcttttc caacatagtc atgtgcatca
    42001 gtttgtatcg cgacacgcca ttccataaat tttattgttg gcgcaacaaa tactttaaca
    42061 gggtgatgct cttgttgaat tgcttttaga gcttgttgct taattacggc tctcatttta
    42121 aaacgaatca tgtaataaat gattagtaaa gcgattatag gaaagaagac tgcaaatgga
    42181 tgcaagccta caacccataa tacaatacca atacaaagaa ctgtgaaaat aatagggtca
    42241 aatgtgttaa tcacacttaa ttgaatccat ttgtttgtga taggtctaag tgcttgtgta
    42301 ccataagaat tgaatatatc tacaaagaca tgtaaaaata ctgcgagctg agcccacatc
    42361 catacatgaa atgggttggt tccactaaag aacgtgaata ttaaaaatgt aattaaaatt
    42421 ggccatagta ttgtgaaagg gatggaatgc gtgatacctc tatgatgcga aatatatgtt
    42481 gcattgtcct ttaatttaag aacagtatcc ccatcaggaa ttaatgaacc aacgataagg
    42541 gttgtagctg ttgcaccaaa cgtagaagcc attgctggat cttgagttgc aagtgctgta
    42601 aggcccaccc caattgcgat atgtgttgca gtgtccatat cagttcactc atttcataat
    42661 attatatata ctattataat ccaatttcgc aaataactaa attaacttac atactaacca
    42721 tgattttaat gtacaataaa aacattatga gtaaagacaa ttgattttgg tgaggaaagg
    42781 gttttataaa tgtatcagca gtctagtttt aaagaaaatt tgatacactg gtttgatgaa
    42841 aatcaaagag aaatgccatg gcgacaaacg accaatccgt actatatttg gttaagtgaa
    42901 gtcatgttac aacagacaca agttaaaaca gttattgact attatcatcg ttttgttgaa
    42961 cgatttccaa ctgtggaagt tttgagtcaa gcttcggaag atgaagtgtt aaagtactgg
    43021 gaaggcttag gatattatag tagagctcga aattttcata cggcaattaa ggaagttcat
    43081 gataaatatg aaggcttagt tcccaaagat cctgatcaat ttaaagcttt aaaaggtgtt
    43141 ggcccataca cacaagctgc agtaatgagc attgcatata atgtaccact agcaactgta
    43201 gacggaaatg tttttcgagt atggtctaga ttgaatgatg actatcgaga tattaaatta
    43261 caatcgacga ggaaatcgta cgaacaagaa ttattaccgt atgttactac agaagctgga
    43321 acatttaatc aagctatgat ggaattaggg gcattaattt gtacaccgaa aaatccatta
    43381 tgcctatttt gtccagttca agaaaactgt gaggcatttg ataaaggtac atttgaaaag
    43441 ttaccagtta aatctaaaaa tgtaagtaaa aaagtaattg agcaatccgt ctttttaatt
    43501 agaaataatc aaggtcaata tctgttgcaa aagcgtagcg aaaaattatt gcatggtatg
    43561 tggcaatttc ctatgtttga aagtgagcat gctagacgtg aaatgactga aaaaatcggt
    43621 catgacattc aaccagtaga aacacctatt tttgaattga agcatcaatt tactcattta
    43681 acatggaaga taaaggtata cgccgtttca ggaacaatta acattgaaag attaccagat
    43741 gacatgattt ggtttgattt aagtgatcga gatcaatata cattccctgt acctatgtca
    43801 aagatttatc aatttataaa tgggtaacat tcgatgattg gtaatattat cggaaaacat
    43861 agtgacatta attttaaaaa tgtataaatg atatctttaa atctcatagg ataacaatgt
    43921 caaaaagtta aagtgttgat ttataaaagt gacgatttta tatactttaa atgggacatg
    43981 taaatctctg ttgtagtact tagcaatatt accgagttta atcattaagt agtaaaatct
    44041 tatttcgtaa aaatatgtat ttatataata ctatttctga aatatgctat aatttataat
    44101 ggttaaatta acagaaggtg gtgtggagta tggtcagaga atccatacct aaagaagggg
    44161 aaaacattaa aattcagagt tataaacatg atggtaagat tcatcgcgtt tggtctgaaa
    44221 caacaatatt aaagggtact gatcatgttg tcattggtgg caatgatcat acgttagtga
    44281 ctgaaagtga cggaagaact tggataacac gtgaaccggc aattgtttac tttcactcag
    44341 aatattggtt taatgttatc tgcatgttta gagaagatgg aatttattac tattgcaatt
    44401 tgtcatcacc atttgtttgt gatgaagagg cattaaaata tatagactat gatttagata
    44461 ttaaagtata tccaaacgga aagtatcatt tattagatga agatgaatat gagcaacata
    44521 tgaatcaaat gaattatccg catgatattg atattatttt aagacgcaac gtggacattc
    44581 ttcaacaatg gatagaacag aagaagggcc cattcgcgcc agattttatc aaagtttgga
    44641 aagaacgtta caaaaaaatt agacagtatt aagcaaataa agtgtaaata ctgattaaat
    44701 catttatatt gacttagtga tgtaaagaag tatgataact tttgctgtcg tgtggggaca
    44761 cagtatccat agatta
//
LOCUS       DAPIDM010000008        41480 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000008 DAPIDM010000000
VERSION     DAPIDM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.008"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            131..1249
                     /gene="ald"
                     /locus_tag="RJE42_000388"
     CDS             131..1249
                     /gene="ald"
                     /locus_tag="RJE42_000388"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDV5875842.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLQDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLATSHDLDYKEILNVIE"
     gene            1344..2384
                     /gene="tdcB"
                     /locus_tag="RJE42_000389"
     CDS             1344..2384
                     /gene="tdcB"
                     /locus_tag="RJE42_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV5875843.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKEKGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2415..3737
                     /locus_tag="RJE42_000390"
     CDS             2415..3737
                     /locus_tag="RJE42_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5875844.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3894..5285
                     /gene="norB"
                     /locus_tag="RJE42_000391"
     CDS             3894..5285
                     /gene="norB"
                     /locus_tag="RJE42_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDV5875845.1"
                     /translation="MEKPSREEFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSTACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIVISLIALVLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLIAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5686..37551
                     /gene="ebh"
                     /locus_tag="RJE42_000392"
     CDS             5686..37551
                     /gene="ebh"
                     /locus_tag="RJE42_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDV5875846.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGLNTSQAQAAETNQ
                     PASALKQKQQNGDTQTENREVEVQNSQNGQSLSAPIENEQPNNNQTNHVDASAVQSST
                     TEHDQPVSQNEQAKKDTAAATPTQSAKAASKHEQSESRAANKKENDNEATHVESHEAN
                     VVTASDSSDSGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKINTLL
                     GSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSSRIQTRSIESRAAEPRSVSD
                     YQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDAKEFALITAK
                     QTGDGYQWVIKFNKGHAPHENMIFWFALPDGQTPVGRTEFVTVNADGTNVQWSNGAGA
                     GANKPLPQMWEYGVNDPHRSHDFKIRNRSGQVIYEWPTVNIKSLSDLARASDYLNEAG
                     ATPATKAFGRQIFDYINGQKPAESSGVPKVYTFIGQGDASYTISFKTQGPTIDKLYYA
                     AGARALEYNQLFMYSQLYVESTQDQQQRLNGLRQVVNRTYRIGTTKRVEVSQGNVQTK
                     KVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYPTNANTNAFRQGGVNPLNDYQLSQLF
                     SDQNFQEAARTKNPIRLMIGFDYPDAFGNGETLVPVNLTVLPEIQHNIKFFKNDDGQN
                     IAEKPASKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQITG
                     IPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTPRIT
                     TTAEELRGTALQKVPVTVTDIPLDPSALVYLVIPTSQTKDGGSEADQIPSGYTKIATG
                     TPDGVHSTITIRPEDYVVFIPPVGHQIRALIFYNNVVASNMSNAVTILPDDIPPTINN
                     PVGLNAKYYRGDEVSFTMGVSDRHSGLKSTTITTLPSGWTSNLTKSDKKNGSLAISGR
                     VSMNQAYNSDITFKVSATDNVNNTTNDSQSKHVTVHVGKISDDAHPIVLGNSEKVVVV
                     NPTALTGDEKQRITTAFMNKNQNIRGYLASSNPVTVDNHGNVTLQYRDGSSTTLDATN
                     VMTYEPVVKPEYQTANAPKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPSSTFTNITS
                     DRTIPTAQEVSQMNAGTQLYHITATNAYHKDTEDFYITLKIIDVKQPEGDQRVYRMST
                     YDITTDEISKVKQAFINANRDAISFAEGDISVTNTPNGSNVSTITVNINKGRLTKSFT
                     SNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGTQIT
                     TNDILTLLKATTTVPGLRNNIAGNEKAQAEAGGRPNYKTTGYSQSNPTSDGQRQFTLN
                     GQVIQIMDIINPSNGFGGQPVTNSNVRANHSNSTVVSVNESAANGAGAFTIDHVVKNN
                     STHNAADAVYKAQLYLSPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTVSVGNY
                     TNHQVFSGETFTNTITANDNFGVQSVTVPNTSQLTGTVDNNHQHVSATAPNVTSATNK
                     TINLVATDTSGNTATTSFNVTIKPLRDKYRVGTSSTAANPVRIANISNNATVSQADQT
                     AIINSLTFTSNAPNRNYATASASEITSKTVSNVSRTGNNAQVTVTVTYQDGTTSTVTV
                     PVKHVIPEIVAHSHYTVQGQDFPTGNGASASDYFKLSNGSAIPDATITWVSGQAPNKN
                     NTTIGQDINVTAHILIDGETTPITKTATYKVVSSVPKHVFETNRGAVFPGISDVYDAK
                     QYVKPVNDSWTQNAQRMNFQFTNSYGPSKDVVGISTRDIRVTYDNHQTQIIKILAKVK
                     PDPPRIDGNSVTYKAGLTNQQIKINNVLSSSSIKLFKADNTPLTITNTTYGSGNTAVV
                     TVSDALPNGVIKARSSITMNNVTYTTQDEHGRAIDVTRNESVDSNDSATVTVTPQLQA
                     TTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVTLPS
                     GQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAINYITFDPNTNTNGITAAWAN
                     RQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTSYTTTVGGTLASGTQ
                     ASGYAHIQNANGLPTDGFTYKWNRDTTGTNDANWAAMNKPNAAKVINAKYDVIYNGHT
                     FATSLLAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVIKRN
                     GNVVTTFTRHNNTSPWVKEASAATVAGIVGTNNGITVAAGTFNAADTIQVVATQGSGE
                     TISDEQLSDDFTVVAPQPNQATTKIWQNGHIDIMPNNPSGHLINPTQAMDIAYTEKVG
                     NGAEHSKTLNAVRGQNNQWTIANKPDYVTLDAHTGKVTFNANTIKPNSAITITPKAGT
                     GHSASSNPSTLTAPATHTVNTTEIVKDYGSNVTPAEINNAVQVANKRTATIKNGTAMP
                     TNLAGGSTTTIPVTVTYNDSSTEEVQESIFTKADKRELITDKNHLDDPVSTDGKKPGT
                     ITQYNNAIHNAQQQINAAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKALLQ
                     NKEDNSQLVTSKNNLQSSVNQVPSTTGMTQQSIDNYNAKKRDAESEITAAQRVIDNGD
                     ATAQQISDEKRRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGITTGKKPASITAY
                     NNSIRALQSDLTSAKNSANAIIQKPIRSVQEVQTALTNVNRVNERLTQAINQLVPLAD
                     NSALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDATDQ
                     QIAAEKTKVEEKYNSLKQAIDGLTPDLAPLQTAKTQLQNDIDQPTNTTGMTSASVATF
                     NEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPLENA
                     KNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKVQQINQVLAGSPTVDQINTNTSA
                     ANQAKSDLDHARQALTPDKAPLQNAKTQLEQSINQPTDTTGMTTASLNAYNQKLQAAR
                     QKLTEINQVLNGNPTVQKINDKVTEANQAKDQLNTARQGLTLDRQPALSTLHGASNLN
                     QAQQNNFTQQINAAQNHAALETIKSNITALNNAMTKLKESVADNNSIKSGQNYTDATQ
                     ANKQAYDNAVNAAKGVIGETNNPTMDVNTVNQKASSVKSTQDALDGQQNLQRAKTEAT
                     NAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHNQVV
                     QSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTNKEHALNGE
                     AKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIETVNTIKQNATNLNSAMGNL
                     RQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVNDVNSATSAV
                     TTNKNALNGDEKLAQSKTDAASAIDALPHLNNAQKADVKSKINVASNIAGVNTVKQQG
                     TDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQNKTK
                     DQVTEVMNQVDSAKTNLDGTRLLDQAKQTAKQQLNNMTHLTPAQKTNLTNQINSGTTV
                     AGVHTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAETII
                     NANSNPEMNPSTITQKADQVNSSKTALNGDENLAAAKQNAKTYLNSLTSITDAQKNNL
                     ISQITSATRVSGVDTVKQNAQHLDQAMANLQSGINNESQVKSSEKYRDADTNKQQEYD
                     NAITAAKAILNKQHGPNTAQNAVEAALQRVNTAKNALNGDAKLIAAQNAAKQHLGTLT
                     HITTAQHNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQNFLD
                     ADEQKRNAYNQAVSNAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNNAKQ
                     AAITAINGSSDLNQHQKEALKAQANGAQRVSNAQDVQRNATELNTAMGTLKHAIADKT
                     TTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQELN
                     GDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNNAMK
                     GLRDSIANETTVKAGQNYTDASPNNQSTYNSAVSNAKGIINPTNNPTMDASAITQAAT
                     QVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTAAKN
                     AATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAETILNKTQGANT
                     PKQDVEASIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKIDQAT
                     TVAGVEAVSNTGTQLNTAMAKLQNGINDKANTLASENYHDADSDKKTAYTQAVTNAEN
                     ILNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTAQKD
                     KLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNAATKNGQNYLDATESNKTN
                     YNNAVDSANSVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNAIDG
                     AANLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQTQKY
                     RDAEQSKKTTYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKLAEA
                     KAAAKQNLSTLNHITNAQRTALEGQINQATTVDGVNTVKTNANTLDGAMNSLQGSIND
                     KDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRAKTA
                     LNGAENLRNAKTTATNTINGLPHLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTLNTA
                     MGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNIDANAINGM
                     ANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKAQVNNAQRVSDANNV
                     QHTATELNGAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGTPNA
                     TLTVNDVNSATSQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQVQS
                     ATTLDGVQTVKNSSQTLNTVMKGLRDSIANEATIKAGQNYTDASLNNRNEYDSAVTAA
                     KAIINQTSNPTMEANTITQATSQVTTKEQALNGAQNLAQAKTTAKNNLNNLTSINNAQ
                     KDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEPSKK
                     SAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAKQTL
                     GTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTLQSQ
                     NYQDADDAKRTAYSQAVNAAATILNKTSGGNTPKADVERAMQTVTQANTALNGIQNLE
                     RAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSVANGVEHTVTELNTAMTALKRAI
                     ADKADTKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPSDVTNAATQVTNAK
                     TQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQTLN
                     TAMKGLRDSIANEATIKAGQNYTDASPNNRSEYDSAVTAAKAIIGQTTSPTMNAQEIN
                     QAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVNEVT
                     QAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILNKAQ
                     GPNTAKDGVETALQNVQRAKNDLNGNQNVANAKTTAKNALNNLTSINNAQKEALKSQI
                     DSATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADSDKQTAYNDAVT
                     AAKTLLDKTAGTNDNKAAVEQALQRVNTAKTALNGDARLNEAKNTAKQQLATMSHLTD
                     AQKGNLTEQIERGTTVAGVQGIQANAGTLDQAMNQLRQSIVSKDATKASEDYHDANTD
                     LQNAYNHAVSEAEGIISASNNPEMNPDTINQKASQVNSANSALNGDEKLAEAKQTAKS
                     DIGRLSDLNNAQQTSATAEVDHAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDNTKR
                     SVNYTDADQPKQQAYDTAVTQAEGITNANGSNANETQVQAALNQLNQAKNDLNGDNKV
                     AQAKEAAKHALASYSNLNNAQSTAATSQIDNATTVAGVTTAQNTANELNTAMGQLQNG
                     INDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKANGQNMTKAQVEAALNQVTTA
                     KNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGVTTVNGVNGVKTKAQDL
                     DGAMQRLESAIANKDQTKASENYIDADPTNKTAFDNAITQAESYLNKDHGANKDKQAV
                     EQAIQSVTSTENALNGDANLQRAKTEATQAIDNLTHLNTPQKTALKQQVNAAQRVSGV
                     TDLKNSATSLNSAMDQLKQAIADHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIVNGS
                     PNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSNAQKQSITGQ
                     IDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDGDTDKQNAYNTAV
                     TSAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQESTANINQLSHLN
                     NAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTDADP
                     EKQTAYNNAVTAAENIINQANGTNENQSQVEAALSTVTTTKQALNGDRKVTDAKNNAN
                     QTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKDTTL
                     GSQNFADADPEKKNAYNEAVRNAENILNKSTGTNVPKDQVEAAMNQVNTTKAALNGSQ
                     NLEKAKQHANTAIDGLSHLTNAQKDALKQLVQQSTTVAEAQGNEQKANNVDAAMDKLR
                     QSIADNATTKQNQNYTDASPNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAGQVS
                     TTKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTNQINGAHTVDEANQIKQNAQ
                     NLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNATQT
                     EVEQAIQQVNTAKQALNGNANVQHAKDEATALINNSNDLNQAQKDALKQQVQNATTVA
                     GVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYKQAVAKAEALIS
                     GTPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRDEYS
                     KQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTAYDN
                     AVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQAAKTNLGTLDH
                     LNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLSNALQDKTETLNSINFTDA
                     DKAKKDAYTNAVAQAEGILSKANGSNASQTEVEQAMQRVNAAKQALNGNDNVHRAKDA
                     AKQVITNANDLNQAQKDALKQQVDAAQTVANVNTNKQTAQDLNQAMTQLKQGVADKDQ
                     TKANGNFVNADTEKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVTDAKNGLNG
                     NHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVTHAELVTGVNAIKQNADALNNAMGT
                     LKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLTPDAVTQAVTT
                     MNQAKDALNGDEKLAQAKQDALANLDTLRDLNQPQRDALRNQINQAQALATVEQTKQN
                     AQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNPTLN
                     PDDITRVLTQVTDAKNGLNGEAKLATEKQNAKDAVNAMTHLNDAQKQALNGQIDQSPE
                     IATVTQVKQTATSLDQAMNQLSQAINDKTQTLADGNYLNADPDKQNAYKQAVAKAEAL
                     LNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQKQS
                     FESQITQAPLVTDVTTINQKAQALDHAMELLRNSIADNQATLASEDYHDAAAQRQNDY
                     NQAVTAANNIINQTTSPTMNPDDVNRATTQVNNTKVALDGDENLAAAKQQANNRLDQL
                     DHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRGNFI
                     NADTDKQTAYTTAVNEAEAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQVAK
                     TNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRESIQDN
                     AATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNVINQAATTVNTTKAALH
                     GDVKLQNDKDHAKQAVSQLAHLNNAQKHMEDTLIDSETTRTAVNHDLTEAQALDQLMD
                     ALQQSIADKDATRASSAYVNAEPNKKQAYDEAVQNAESIIAGLNNPTINKGNVTSATQ
                     AVTSSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAEIIA
                     QAQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTDPTL
                     VKSVIDQATQAVNDAKNNLHGDQKLAQDKQRATETLNNLSNLNTPQRQALENQINNAA
                     TRGEVAQKLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQHAKD
                     LINQTSNPTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQ
                     ALESQINNAATRDEVAQKLAEAKALNQAMEALRNSIQDQQQTESGSKFINEDKPQKDA
                     YQAAVQHAKDLINQTGNPTLDKSQVEQLTQAVATAKDNLHVDQKLVRDQQQAVTTVNA
                     LPNLNHAQQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNY
                     TEASTDKKDAVDQALQAAQSITDPTNGSNANKYAVEQALTKLQGKENELNGNERVAEA
                     KTQAKQTIDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNE
                     HANVEQTVDYTQADLDKQNAYKQAIAEAENVLKQNANKQQVDQALQNILNAKQALNGD
                     ERVALAKTNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMNQL
                     SQEITGNEGRTKGSTNYVNADTQVKRVYDEAVDKAKEALNKATGQNLTAEEVIKLNDA
                     VTAAKQALNGEERLNNRKSEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINR
                     AVQLDDAMGAVQQYIDEHHLDVISSTNYINADDNLKANYDNAITNAAHELDKVQGSAI
                     AKAEAEQLKQHIIDAQKALNGDQNLATAKDKANAFVDTLNGLNQQQQDLAHQAINNAD
                     TVTGIANIINDQIDLNNAMETLKHLVDNEIPTAEQTVNYQNADDVAKSNFDDAKRLAN
                     ALINSDNTNVNDINGAIQAVKDVIQNLNGEQRLQEAKDKAIQNVNKVLADKLKEIEAS
                     NATDQDKLIAKNKAEELANSIINNINKATSNQDVSRVQTAGNQAIEQVHANEIPKAKI
                     DANKDVDKQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEHA
                     KAQLAQALQDIKDLVKAKENAKQDVDKQVQALIDEIDRNPNLTDKEKQALKDRINQIL
                     QQGHNGINNAMTKEEIEQAKAQLAQALQDIKDLVKAKENAKQDVDKQVQALIDEIDRN
                     PNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQALQDIKDLVKAKED
                     AKNAIKALANEKRDQINSNPDLTPEQKAKALKEIDEAEKRALENIENAQTKDQLNKGL
                     NLGLDDIRNTHVWEVDAQPAVNEIFDATPEQILVNGELIVHRDDIITEQDIHAHINLI
                     DQLTAEIIDTPSTATISDSLTAKVEVTLLDGSKVIVSVPVKVVEKELTVVKQQAIESI
                     ENAAQQKINEINNHATLTPEQKEAAIAEVNKLKQQAIEQINNAADVHTVEEVQHQEQA
                     HIEQFNPDQFTIDQAKSNAIKSISDAIQHMIDEINASKDLTDKEKQEAISKLNQLKDQ
                     SIQAIQRAQSIDEIAQQLEQFKAQLKAANPFAKELENRKKSAISKIKDISTDEINRIR
                     NSTIGTAEERQAAMNRINEIVLETIRDINNAHTPQQVEAALNNGIARILAVQIVTSDH
                     SKPSSNSDGQSNSHLHVGYGTVNHPYNSTTIGHKKKLDQDDEIDPLHMRHFGDRIGNV
                     IKNALGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNSKDKKKGSVEGTKHLPLLF
                     AKRRRKEDEEDAIVEEKDSLNNDESLDKVKHTPFFLPKRRRKEDEEDVEVTNENTDEK
                     VLQDNEHSPILIAKRRRDKEEDVETTSSIDSKDEDVPVLLAKKKNQKDNQSKGKKSAS
                     KKPSKKVAAKKKKKKSKKNKK"
     gene            complement(37610..38011)
                     /locus_tag="RJE42_000393"
     CDS             complement(37610..38011)
                     /locus_tag="RJE42_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDV5875847.1"
                     /translation="MAKINFDAATKGNPGISTCAVVIKEDEQHFTYTHELGEMDNHTA
                     EWAACIYALKHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(38247..38951)
                     /locus_tag="RJE42_000394"
     CDS             complement(38247..38951)
                     /locus_tag="RJE42_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDV5875848.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRKVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVINVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            39196..39390
                     /locus_tag="RJE42_000395"
     CDS             39196..39390
                     /locus_tag="RJE42_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDV5875849.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            39402..39653
                     /locus_tag="RJE42_000396"
     CDS             39402..39653
                     /locus_tag="RJE42_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDV5875850.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            39691..40815
                     /locus_tag="RJE42_000397"
     CDS             39691..40815
                     /locus_tag="RJE42_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDV5875851.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAVFSGESQVMETGKDPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTEHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLVTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDQALGSVWKQMTNRTI"
     gene            40831..41268
                     /locus_tag="RJE42_000398"
     CDS             40831..41268
                     /locus_tag="RJE42_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDV5875852.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGRDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        16341 a         7169 c         7535 g        10435 t
ORIGIN      
        1 ataatatatt aaatatttat taacaaatta tagataaaat atgaataatt aattaatggt
       61 atttacatat gcatacccac aagataaaat gattgtgaaa taattcacaa aatcaaaagg
      121 agtgttgcaa atgttagttg cagtagtcaa agaattaaaa caaggtgaag gacgtgtagc
      181 ttgcacaccc gaaaatgtgc gtaagttaac ggatgcagga cataaagtaa ttgttgaaaa
      241 aaatgctggc attggttcag gattttctaa cgatatgtat gaaaaagaag gcgctaagat
      301 cgtaactcac gaacaagcat gggaagcaga tcttgttatc aaagtaaaag aacctcatga
      361 aagcgaatat caatatttca aaaagaatca aatcatctgg ggatttttac atctagcatc
      421 ttcaaaagaa atagtagaaa aaatgcaaga agttggtgtt actgcgatta gtggtgaaac
      481 cattataaaa aatggaaaag ccgaattatt agcgccaatg agtgctatag caggtcaacg
      541 ctcagcaatt atgggagctt actactctga agcacaacat ggtggtcaag gtactttagt
      601 gactggtgta catgaaaatg tggatatacc tggtagtaca tatgtgattt tcggtggtgg
      661 cgtagcagca acaaatgcag ccaatgttgc cttgggacta aatgctaaag taatcattat
      721 cgagttaaac gatgatcgca ttaaatattt acaagacatg tatgcagaaa aagatgtcac
      781 agtagtcaaa tcaacaccag aaaatttagc agaacaaatt aaaaaagcag atgtatttat
      841 ttctacaatt ttaattccag gtgcgaaacc gccaaaattg gttactcgtg agatggttaa
      901 atcaatgaaa aaaggttcag tattaatcga tatagctatt gaccaaggtg gaactattga
      961 aacaattaga ccaactacaa tttctgatcc agtgtatgaa gaagaaggcg taattcatta
     1021 cggtgtacca aaccaaccag gtgcagttcc aagaacgtca acaatggcat tagcacaagg
     1081 aaatattgat tatatattag aaatttgtga caaaggctta gaacaagcaa ttaaagataa
     1141 tgaagcctta agtactggtg taaacattta ccaaggacaa gtgacaaacc aaggattagc
     1201 tacatcacat gacctagatt ataaagaaat attaaatgtt attgaataga tagtaatata
     1261 aacgaaattg agtgaaatga atattttaaa tatagcatta tagtttggac taaaaattta
     1321 caaaacggaa ggatgtaatt attatgacaa ccaacacagt tacattacaa acagcacaca
     1381 ttgtaagttt aggagatatc gaagaagcta aagcaagcat taaaccattt attcgtcgaa
     1441 ctcctctaat taaatctatg tatttaagcc aaagtataac taaaggaaat gtatttctaa
     1501 aattagaaaa tatgcaattc acaggatctt tcaaatttag aggagctagc aataaaatta
     1561 atcacttaac agatgaacaa aaagaaaaag gcattatcgc agcatctgct gggaaccatg
     1621 cacaaggtgt tgctttaaca gctaaattac taggcattga tgcaactatc gtaatgcctg
     1681 aaacagcacc acaagcgaaa caacaagcaa caaaaggcta tggggcaaag gttattttaa
     1741 aaggtaaaaa ctttaacgaa actagacttt atatggaaga attagcgaaa gaaaaaggca
     1801 tgacaatcgt tcatccatat gacgataagt ttgtaatggc aggtcaagga acaattggtt
     1861 tagaaatttt agatgatatt tggaatgtga atacagtcat cgtaccagtt ggcggtggcg
     1921 gattaattgc aggtattgcc accgcattaa aatcatttaa cccttcaatt catattatcg
     1981 gtgttcaatc tgagaatgtt catggtatgg ctgaatcttt ctataagaga gatttaactg
     2041 aacatcgagt ggatagcaca atagcagatg gttgtgatgt aaaagttcct ggtgaacaaa
     2101 catatgaagt agttaaacat ttagtagatg aatttattct tgttactgaa gaagaaattg
     2161 aacatgctat gaaagattta atgcagcgtg ccaaaattat tactgaaggt gcaggcgcat
     2221 taccaacagc tgcaatttta agtggaaaaa taaacaataa atggcttgaa gataaaaatg
     2281 ttgttgcttt agtttcaggc gggaatgttg acttaactag agtttcaggt gtcattgagc
     2341 atggactgaa tattgcagat acaagcaagg gtgtggtagg ttaaaacatt taatcttaaa
     2401 aatgaggtgt aattatgtca aatggtaaag aattacaaaa aaatataggt ttcttctcag
     2461 cgtttgctat tgttatgggg acagttattg gttcaggagt attctttaaa atatcaaacg
     2521 taacagaagt aacaggaaca gcaggaatgg ccttgtttgt atggttccta ggcggcatca
     2581 ttaccatttg tgcggggtta acagcagcag aacttgctgc tgcaatccct gaaacaggtg
     2641 gcttaacaaa gtatatagaa tatacatacg gtgatttctg gggcttccta tcaggttggg
     2701 cgcaatcatt tatttatttt ccagctaacg tagcagcatt gtctatcgta tttgcgacac
     2761 agttaattaa tttattccat ttatctatag gttcgttaat accgatagca atcgcatctg
     2821 cgttatctat tgtgttgatc aatttcctag gttcaaaagc aggcggaatt ttacaatcag
     2881 ttacattagt aattaaactg attccaatca tcgttatcgt aatttttggt atatttcaat
     2941 ctggagatat cactttttca ttaattccaa ctacaggtaa ttcaggaaat ggtttcttta
     3001 cagcaattgg tagtggttta ttagcaacta tgtttgcata tgatggttgg attcatgtag
     3061 gaaatgttgc gggggaactt aaaaatccta aacgcgattt acctttagcg atttcagttg
     3121 gtatcggttg tattatggct gtgtatttat taattaacgc tacattctta ttaacactgc
     3181 caatagaact acttgctggt aatttaaatg cagcttcaga tacatcaaaa atattatttg
     3241 gtgaaaatgg cggtaagatt attacaatcg gtatattaat ttctgtttat ggtacgatca
     3301 atggctatac tatgactggt atgcgcgtac catatgcaat ggctgaaaga aaattattgc
     3361 catttagcca cttattcgca aaattaacaa aatctggcgc accatggttt ggcgcaatta
     3421 tacaacttat aatcgctatc atcatgatgt caatgggtgc ctttgataca attacaaaca
     3481 tgttaatttt tgttatttgg ttgttctatt gtatgtcgtt tgttgcggta ataattttaa
     3541 gaaaacgtga accaaatatg gagcgaccat ataaagtccc gttatatcca atcatacctt
     3601 taattgctat tttggcagga tcatttgtat taattaatac actgtttaca caatttatat
     3661 tggcaattat tgggattcta atcacagcac ttggtatacc agtttattac tataaaaaga
     3721 aacaaaaagc agcataaggt aagataacta gcattgagaa taaatggatg gactactaat
     3781 aaatttaaag gtttgcacat taaaatcaaa aaccattcaa ttattctatc gaacagacaa
     3841 atttctgtta tggaatttgt ctgtttttca aaagtatagg gaggcaaata gagatggaaa
     3901 agccgtcaag agaggaattt gaaggcaata ataagttgtt aataggaatt gttttaagtg
     3961 taataacgtt ttggctattt gcacaatcat tggttaatgt tgtaccaata cttgaagata
     4021 gtttcaatac agatattgga acggttaata tcgccgttag tataactgct ttattttcag
     4081 gaatgtttgt agtaggagca ggtggtcttg ctgataaata tggcagaatt aaactcacga
     4141 acattggtat tatcttaaat atattgggtt cattattaat cattatttca aatattcctt
     4201 tattacttat tataggaaga ttaattcaag gactttcaac agcatgtatt atgcctgcaa
     4261 ctttgtctat tattaagtca tattacattg ggaaagatag acaacgcgct ttaagttatt
     4321 ggtcaattgg ctcatggggc ggctctggtg tttgttcatt ttttggaggt gcagttgcaa
     4381 cgcttttagg ttggcgttgg attttcatcc tatcaattgt aatttcatta attgcgctgg
     4441 ttcttattaa aggcacacct gaaactaaat ctaaatcgat ttctctaaat aaatttgaca
     4501 ttaaaggtct ggttctttta gtcattatgc tcctcagttt aaatatttta attactaaag
     4561 ggtcagaatt aggtgtaacc tcacttcttt ttatcacttt aatagcaatt gcaattggat
     4621 cttttagttt atttatagtt cttgaaaagc gtgctacaaa tcctttaatc gattttaaat
     4681 tatttaaaaa taaagcttat acaggtgcaa cagcttcaaa ctttttgtta aatggtgttg
     4741 cgggaacgtt aatagtagcc aacacatttg ttcaaagagg tttaggatat tcttccttgc
     4801 aagcaggaag tttatcaatc acttatttag taatggtact aattatgatt cgggttggtg
     4861 aaaagttact tcaaacactc ggatgcaaga aaccaatgct aattggaaca ggagttctta
     4921 ttgtcggaga atgtctcatt tcattaactt tcttgccaga aatattatat gtcatttgtt
     4981 gtataatagg ttatttattc tttggtttag gactaggaat atatgctaca ccatcaacag
     5041 atacagcaat tgcaaatgca ccgttagaaa aagtaggcgt tgctgcaggt atctataaaa
     5101 tggcttctgc attaggtgga gcatttggcg tcgcattgag tggtgcagtt tatgcaatcg
     5161 tatcaaatat gacaaacatt tatactggtg caatgattgc attatggtta aacgcaggta
     5221 tgggaatttt atcattcgtt atcattttgt tacttgtgcc taaacaaaac gacactcaat
     5281 tatgataatt gagaattaaa ttgaaatcat aaaggtcgct acaatattaa acaaaaatat
     5341 aaaccgattc ttatgtgtaa tcattataaa tgattctaca taaggtcggt tttttattac
     5401 ccttttacac tactttattt ataattatta taaattgtca caaattcaat ttacctatca
     5461 atatattttg tgttattata ttctggagca taaataaatt gttcaacaca tagttgtaat
     5521 gtatttcaat actttttaga tagattgcga aattgtattg aatcgtcatt gttttaaatt
     5581 tttaaatgag aatggaatga gcattacaat acacatgcaa tcaaatgtaa atacattcac
     5641 aacacaacag agacataaca acaagataag gagtgaacaa tagctgtgaa ttatcgtgat
     5701 aaaattcaaa agtttagtat tcgtaagtat accgttggaa cattctcgac ggtcattgcg
     5761 acgttagtat ttctagggtt aaacacatca caagcacaag ctgctgagac aaatcaacca
     5821 gctagtgcat taaaacagaa acaacaaaat ggtgatacgc agactgagaa tcgagaagta
     5881 gaagttcaaa attcacaaaa tggtcaatca ttatctgcgc ctattgaaaa tgaacaacca
     5941 aataataatc aaacaaacca tgtagatgct agtgcagtac aatcatctac aacagaacat
     6001 gaccaaccag tatctcaaaa tgagcaagca aagaaagata cggcagctgc tacgccaaca
     6061 caatcggcta aagcggcaag taagcatgaa caaagcgaaa gtcgtgctgc taacaaaaag
     6121 gaaaacgaca atgaagcaac acatgtagaa agtcatgaag caaatgtagt cacagcttca
     6181 gattcatcag atagtggtaa cgtacaacac gatcgtaatg aattacaagc attttttgat
     6241 gctaattacc atgattatcg ctttattgac cgcgaaaatg cagattctgg gacatttaac
     6301 tatgtaaaag gcatttttga caagatcaat acgttattag gtagtaatga tccaataaac
     6361 aataaagact tgcaacttgc atataaagaa ttagaacaag ctgttgcttt aattcgtaca
     6421 atgcctcaac gtcaacaaac aagtcgacgc tcaagtagaa ttcaaacgcg ttcgattgaa
     6481 tcaagagctg cagaaccaag atcagtatca gactatcaaa acgcaaactc atcatattat
     6541 gttgaaaatg ctaatgatgg ttctgggtat cctgtaggta catatattaa tgcttctagt
     6601 aagggggcac catataattt acctactacg ccatggaata cgttaaaagc atcagacgca
     6661 aaagaatttg cacttatcac agcaaagcaa actggagacg gttatcaatg ggtgattaag
     6721 tttaacaaag gtcacgcacc acatgaaaat atgattttct ggtttgcact accagacgga
     6781 caaactcctg ttggtagaac ggagtttgta actgtgaatg cagacggtac aaatgtacaa
     6841 tggagtaatg gtgcaggagc cggtgcgaac aaaccacttc cacaaatgtg ggaatacggt
     6901 gtaaatgatc cccatcgttc acatgacttt aaaataagaa atagaagcgg gcaagtaata
     6961 tatgaatggc caactgtcaa tattaaaagc ttgagcgatt tagcacgtgc aagtgattat
     7021 ttaaatgaag ctggagcaac acctgctact aaagcttttg gtagacaaat attcgattat
     7081 attaatggtc aaaaacctgc tgaatcatct ggtgtaccaa aagtttatac tttcatcggt
     7141 caaggtgatg cgagttatac gatttcattt aaaacgcaag gtccaacaat tgataaattg
     7201 tattatgctg ccggtgctcg tgcattagaa tacaatcaat tatttatgta tagtcagtta
     7261 tatgtcgaat caacacaaga ccagcaacaa cgtcttaatg gtttaagaca agtggttaat
     7321 cgtacttatc gtataggtac aactaaacga gtagaagtga gtcaaggaaa tgtacaaaca
     7381 aaaaaggtat tagaaagtac gaacctaaat atagatgatt ttgtagatga tccattaagt
     7441 tatgttaaga caccaagtaa taaagtatta ggtttttatc caactaatgc aaacactaac
     7501 gcttttagac agggtggagt caatccatta aatgattatc aattaagtca attatttagt
     7561 gatcaaaatt ttcaagaagc agcaagaact aaaaatccaa tacgattaat gattggtttc
     7621 gactatcctg atgcctttgg taacggtgaa actttagtgc ctgttaattt aacagtatta
     7681 cctgaaattc aacataacat taaattcttt aaaaatgatg atggtcaaaa tattgctgaa
     7741 aaacctgctt caaaacaagc tggacatccg gttttctatg tatatgcagg taatcaaggg
     7801 aatgcttcag tgaacttagg tggtagtgta acatctatac aaccgctacg tattaattta
     7861 acaagtaatg aaaactttac agataaagat tggcaaatta caggtattcc acgtacatta
     7921 cacattgaga attcaacaaa tagacctaat aatgccagag aacgtaacat tgaactggtt
     7981 ggtaatttat taccaggcga ttactttgga acaatacgat ttggacgtaa agaacaatta
     8041 ttcgagattc gcgttaagcc acatacgcct agaattacta caacagcaga agaattaaga
     8101 ggtacagcat tacaaaaggt acctgtaacc gttacggata ttccattgga tccatctgca
     8161 ttagtatatt tagttatacc tacgtctcaa acaaaagatg gtggttctga ggcagatcaa
     8221 ataccatcag gttatacaaa aattgcgacg ggtacacctg atggggttca ttcaacgata
     8281 acgatacgac cagaagatta tgtagtattt atacctccgg taggtcatca aattagggcg
     8341 ttaatatttt acaataatgt agttgcctca aatatgagta atgcagttac aattttaccg
     8401 gatgatattc caccgacaat taataaccca gttggtttaa atgcgaaata ttatcgtggc
     8461 gatgaagtta gctttactat gggggtttca gatagacatt ctggattaaa aagtacaact
     8521 attacgacat tgccgagtgg ttggacatca aatttaacta aatccgacaa gaaaaacggc
     8581 tcattagcta tttcaggtag agtctctatg aatcaggctt ataatagtga tattacattc
     8641 aaagtgtcag caaccgacaa tgtcaataat acgacgaatg atagtcagtc taaacatgtg
     8701 acagtgcatg taggtaaaat tagtgatgat gcgcatccaa ttgtcttagg aaattctgaa
     8761 aaagttgtcg ttgtaaatcc gactgcttta actggtgatg aaaagcaacg tattactact
     8821 gcgtttatga ataaaaacca aaatataaga ggttatttag cttcatcaaa tccagttaca
     8881 gtagataatc atggtaatgt cacattgcaa tatcgtgatg ggtcatcaac tacgcttgat
     8941 gcgacaaacg tgatgacata cgaaccagtt gttaagcctg aatatcaaac tgctaatgca
     9001 cctaaaacgg caacggttac aattgctaaa ggacaatcat ttaatattgg agatattaag
     9061 caatatttca ctttaagtaa tggacaagcg attccaagca gtacatttac aaacattaca
     9121 tcggatagaa cgattccaac tgcacaagaa gttagtcaaa tgaacgctgg tacacagcta
     9181 taccacataa ctgctacaaa tgcgtatcat aaagacactg aagatttcta tatcactttg
     9241 aaaatcattg atgtgaaaca accagaaggt gatcaacgtg tatatcgtat gtcaacatat
     9301 gatataacta ctgatgaaat ttcaaaagtg aaacaagcat ttatcaatgc caatagagat
     9361 gcgatttcat ttgcagaagg agatatttca gtaacaaata cacctaatgg ttctaacgtg
     9421 agtaccatta ctgtcaatat taataaaggt cgtttaacga aatcgtttac atcaaacctt
     9481 gctaatatga atttcttgcg ctgggttaac ttcccgcaag attatacagt gacatggaca
     9541 aatgcaaaaa ttgcaaatag accgactgat ggtggtttat catggtctga tgatcataaa
     9601 tcattaattt atcgctatga tgcaacatta ggtacgcaaa ttacgacgaa tgatatttta
     9661 actttgttaa aagcgacaac aacggtgcct ggattacgta ataacattgc tggtaatgaa
     9721 aaagcacaag cagaagctgg tggtagacca aactataaaa caactggtta ttcacaatca
     9781 aatccaacat ctgatgggca acgacaattt acgttgaatg gtcaagtcat tcaaataatg
     9841 gatattatta atccttcaaa tggatttggt gggcaacctg ttacaaattc aaatgtccgt
     9901 gcgaaccaca gtaactcgac tgtcgttagt gttaatgaat cagcagctaa tggtgctgga
     9961 gcatttacaa ttgatcatgt agtaaaaaat aattctacgc ataatgcagc tgatgcggta
    10021 tataaagcgc agttatactt atcgccatat ggtccaaaac aatatgttga acatttaaat
    10081 caaaatacgg gaaatactac tgatgctatt aacatttatt ttgtacctag tgatctagta
    10141 aatccaactg tttcagtagg taattacaca aatcatcaag tattctctgg tgaaacattt
    10201 accaatacga ttacagcgaa tgataacttt ggtgtgcaat ctgtaactgt cccaaataca
    10261 tcacaattga caggcactgt tgataataac catcaacatg tatcagcaac ggcaccaaat
    10321 gtgacatcag ctacaaataa aacaattaat ttggtggcaa ctgatacaag cggtaatact
    10381 gcaacaacgt cattcaatgt cacaattaaa cctttacgtg ataaatatcg agttggaaca
    10441 tcatcaacgg ctgctaatcc agtgagaatt gccaatattt cgaataatgc gacagtatcg
    10501 caagcagatc aaacagcaat tattaattca ttaacgttta caagcaatgc accaaataga
    10561 aactatgcaa cagcaagtgc atctgaaatc actagtaaaa cagtgagtaa tgtgagtcgt
    10621 acaggaaata acgcacaagt aactgtaact gttacttatc aagatggaac aacatcaaca
    10681 gtgactgtac ctgtaaagca tgttattcca gaaatcgttg cacattcgca ttacactgta
    10741 caagggcaag atttcccaac aggtaatgga gcaagtgcat ctgattactt taaattatcg
    10801 aatggtagtg ccattccaga tgcgacgata acatgggtaa gtggtcaagc gccaaacaaa
    10861 aataacacaa ctataggtca agatatcaat gtaactgcac atatcttaat tgatggtgaa
    10921 acaaccccaa ttacgaaaac agcaacatat aaagtagtaa gttcagtacc gaagcatgtc
    10981 tttgagacga atagaggcgc tgtgttccct ggtatatctg atgtgtatga tgcgaaacaa
    11041 tatgttaaac cagttaatga ttcttggaca caaaatgcgc aacgtatgaa tttccaattt
    11101 acaaattcat atggaccaag taaagatgtg gttggtattt caacaaggga tattcgagtt
    11161 acttatgata atcaccaaac gcaaattatc aagattttag caaaagttaa accagatcca
    11221 ccgcgaattg atggcaactc tgtgacttat aaagcaggtc ttactaacca acaaattaaa
    11281 attaataatg tgttaagtag ttcatctatt aaattattta aagcagataa tacgccatta
    11341 acgattacaa acacgactta tggtagtggt aatactgcag ttgtaacggt aagtgatgcg
    11401 ttaccaaatg gtgtgattaa ggcaaggtct tctatcacaa tgaataatgt gacgtacact
    11461 actcaagatg aacatggtcg agcgatagat gtaacaagaa atgaatctgt tgattcaaat
    11521 gacagtgcaa cagtaacagt gacaccacaa ttacaagcaa ctactgaagg cgctgtattt
    11581 attaaaggtg gcgacggttt tgatttcgga cacgtagaaa gatttattca aaatccgcca
    11641 catggggcaa cggttgcatg gcatgatagt ccagatacat ggaagaatac agtcggtaac
    11701 actcataaaa ctgcggttgt aacattacct agtggtcaag gtacgcgtaa tgttgaagtt
    11761 ccagtcaaag tttatccagt tgctaatgct aaggcgccat cacgtgatgt gaaaggtcaa
    11821 aatttgacta atggaacgga tgcgataaac tacattacat ttgatccaaa tacaaacaca
    11881 aatggtatca ctgcagcatg ggcaaataga caacaaccaa ataaccaaca agcaggtgtt
    11941 caacatttaa atgtcgatgt gacatatcca ggtatttcag ctgcaaaacg tgtacctgtt
    12001 actgttaatg tatatcaatt tgaattccct caaacttctt atacaacaac agttggtggc
    12061 actttagcaa gtggtacgca agcatcagga tatgcacata tacaaaatgc taatggttta
    12121 ccaacagatg gatttacgta taaatggaat cgtgatacta caggtacgaa cgatgcaaac
    12181 tgggcagcaa tgaataaacc gaatgcggct aaagtcatta atgcgaaata tgatgtcatc
    12241 tataatggac atacatttgc aacatctcta ctagcgaagt ttgtagtaaa agatgtccaa
    12301 ccagcgaaac caactgttac tgaaacggca gcaggagcaa ttacgattgc acctggagca
    12361 aaccaaacag tgaatacaca tgccggtaac gtaacaacat acgctgataa attagttatt
    12421 aaacgtaatg gtaatgttgt gacgacattt acacgtcaca ataatacgag tccatgggtg
    12481 aaagaagcat ctgcagcaac tgtagcaggt attgttggaa cgaataatgg tataactgtt
    12541 gcagctggta ctttcaatgc tgctgataca attcaagttg ttgcaacaca aggtagtggt
    12601 gaaacaatca gcgacgaaca acttagtgat gatttcacag ttgtcgcacc tcaaccgaac
    12661 caagcgacta ctaagatttg gcaaaatggt catattgata tcatgcctaa taacccatca
    12721 gggcatttaa ttaatccaac tcaagcaatg gatattgctt acactgaaaa agtgggtaat
    12781 ggtgcagaac atagtaagac acttaatgct gttcgtggtc aaaataacca atggacaatc
    12841 gccaataaac ctgactatgt aacgttagat gcacatacag gtaaagtgac gttcaatgcc
    12901 aatactatca agccgaattc agcaattacc attacaccga aagcaggtac aggtcactca
    12961 gcaagtagta atccaagtac attaactgca ccggcaactc atactgtcaa cacaactgaa
    13021 attgtaaaag attatggttc aaatgtaaca cctgctgaaa ttaacaatgc agttcaagtt
    13081 gctaataaac gtactgcaac gattaaaaat ggcacagcaa tgcctactaa tttagctggt
    13141 ggtagtacaa cgacgatccc tgtgacagta acttacaatg atagcagtac tgaagaagta
    13201 caagagtcca ttttcacaaa agcggataaa cgtgagttaa tcacagataa aaatcattta
    13261 gatgatccag ttagtacaga tggtaaaaag ccaggtacaa ttacgcagta caataatgca
    13321 attcataatg cgcaacaaca aatcaatgct gcgaaaacag aagcgcaaca agttattaat
    13381 aatgagcgtg caacaccaca acaagtttct gacgcattaa ctaaagttcg tgcagcacaa
    13441 actaagattg atcaagctaa agcattactt caaaataaag aagataatag tcaattagta
    13501 acgtctaaaa ataacttaca aagttctgtg aaccaagtac catcgactac tggtatgacg
    13561 caacaaagta ttgataacta taatgcgaag aaacgtgatg cagaatctga aataactgca
    13621 gctcaacgtg ttattgataa tggtgatgca actgcacaac aaatttcaga tgaaaaacgt
    13681 cgtgtcgata acgcattaac agcattaaac caagcgaagc atgatttaac tgcagataca
    13741 catgccttag agcaagcagt gcaacaattg aatcgcacag gtataacgac tggtaagaaa
    13801 ccagcaagta tcactgcgta taataattcg attcgtgcac ttcaaagtga cttaacaagt
    13861 gctaaaaata gtgccaatgc tatcattcag aagccaatta gatcagtaca agaggtacaa
    13921 acagcattaa caaatgtaaa tcgtgtcaat gagcgattaa cgcaagctat taatcaatta
    13981 gtacctttag ctgataatag tgctttaaga acagctaaga cgaaacttga tgaagaaatc
    14041 aataaatcag taactactga tggtatgaca caatcatcaa tccaagcata tgaaaatgct
    14101 aaacgtgcgg gtcaaacaga atcaacaaat gcacaaaatg ttattaacaa tggtgacgca
    14161 acagaccaac aaattgccgc agaaaaaact aaagtagaag aaaaatataa tagcttaaaa
    14221 caagcaattg atggattaac accagacttg gcgccattac aaactgcgaa aactcagttg
    14281 caaaatgata ttgatcaacc aacgaatacg acaggtatga caagtgcatc tgttgcgaca
    14341 tttaatgaaa aactttcagc agcaagaact aaaattcaag aaattgatcg cgtactagca
    14401 tctcatccag atgttgcaac gattcgtcaa aacgtgacag cagcgaatgc tgctaaatca
    14461 gcacttgatc aagcgcgtaa tggcttaaca gtcgataaag cacctttaga aaatgcgaaa
    14521 aaccaactac aacatagtat tgatacgcaa acaagtacaa ctggtatgac gcaagactct
    14581 ataaatgcat acaatgcgaa gttaacggct gcacgtaata aggttcaaca aatcaatcaa
    14641 gtattagcag gttcacctac tgtagatcaa attaatacaa atacgtctgc agcaaatcaa
    14701 gcgaaatctg atttggatca tgcgcgtcaa gcgttaacac cagataaagc gccgcttcaa
    14761 aatgcgaaaa cgcaattaga acaaagcatt aatcaaccaa cggatacaac aggtatgacg
    14821 accgcttcgt taaatgcgta caaccaaaaa ttacaagcag cgcgtcaaaa gttaactgaa
    14881 attaatcaag tgttgaatgg caacccaact gtccaaaaaa tcaatgataa agtgacagag
    14941 gcaaaccaag ctaaggatca attaaataca gcacgtcaag gtttaacatt agatagacag
    15001 ccagcgttat caacattaca tggtgcttct aacttaaacc aagcacaaca aaataatttc
    15061 acgcaacaaa ttaatgctgc tcaaaatcat gctgcgcttg aaacaattaa gtctaacatt
    15121 acagctttaa ataatgcgat gactaaatta aaagaaagtg ttgcagataa taattcgatt
    15181 aaatcaggtc aaaattatac tgacgcaact caagctaata aacaagcgta tgataatgca
    15241 gttaatgcgg ctaaaggtgt cattggagaa acgaataatc caacgatgga tgttaacaca
    15301 gtgaaccaaa aagcatcatc tgtgaaatcg acgcaagatg ctttagatgg tcaacaaaac
    15361 ttacaacgtg cgaaaacaga agcaacaaat gcgattacgc atgcgagtga tttaaaccaa
    15421 gcgcaaaaga atgcattaac acaacaagta aatagtgcac aaaatgtaca agcagtaaat
    15481 gatattaaac aaacgactca aagcttaaat actgctatga caggtttaaa acgtggcgtt
    15541 gctaatcata atcaagtcgt acaaagtgat aattatgtca acgcagatac taataagaaa
    15601 aatgattaca acaatgcata caatcatgcg aatgacatta ttaacggtaa tgcacaacat
    15661 ccagttataa caccaagtga tgttaacaat gctttatcaa atgtcacaaa taaagaacat
    15721 gcgttgaatg gtgaagctaa gctaaatgct gcgaagcaag aagcgaacac tgcattagga
    15781 catttaaata acttgaataa tgcacaacgt caaaatttac aatcgcaaat taatggtgcg
    15841 catcaaatcg agacagttaa tacaattaag caaaatgcaa ctaacttaaa tagtgcaatg
    15901 ggtaacttaa gacaagctgt tgcagataaa gatcaagtga agcgtacaga agattatgcg
    15961 gatgcagata cagctaaaca aaatgcatat aatagcgccg tttcaagtgc tgaaacaatc
    16021 attaatcaaa caacaaatcc aacgatgtct gttaatgatg ttaacagtgc aacttcagct
    16081 gttactacta ataaaaatgc attaaatggt gatgaaaaat tagcacaatc taaaacagat
    16141 gctgcaagtg caattgatgc attaccacat ttaaataatg cacaaaaagc agatgttaaa
    16201 tctaaaatta atgttgcatc aaatattgct ggtgtaaata ctgttaaaca acaaggtaca
    16261 gatttaaata cagcgatggg taatttgcaa ggtgcgatca atgatgaaca aacgacattg
    16321 aatagtcaaa attatcaaga tgcgacacca agtaagaaaa cagcatacac aaatgcggta
    16381 caagctgcga aagatatttt aaacaaatca aatggtcaaa ataaaacgaa agatcaagtt
    16441 actgaagtga tgaatcaagt ggattcggct aaaactaact tagatggtac acgtttatta
    16501 gatcaagcga agcaaacagc gaaacagcag ttaaataata tgacgcattt aacacctgct
    16561 caaaaaacga atttaacaaa tcaaattaat agtggtacga ctgtcgctgg tgttcatacg
    16621 gttcaatcaa atgcgaatac attagatcaa gcgatgaata cgttaagaca aagtattgcc
    16681 aacaaagatg caactaaagc aagtgaagat tacgtagatg ctaataatga taagcaaaca
    16741 gcatataaca acgcagtagc tgctgctgaa acgattatta atgcaaatag taatccagaa
    16801 atgaatccaa gtacgattac acaaaaagca gatcaagtga atagttctaa aacggcactt
    16861 aacggtgatg aaaacttagc tgctgcaaaa caaaatgcga aaacgtactt aaactcattg
    16921 acgagtatta cagatgctca aaagaacaat ttaattagtc aaattactag tgcgacaaga
    16981 gttagtggtg ttgatactgt aaaacaaaat gcgcaacatc tagaccaagc tatggctaac
    17041 ttgcaaagtg gtattaacaa cgaatctcaa gtgaaatcat ctgagaaata tcgtgatgct
    17101 gatacaaata aacaacaaga gtatgataat gctattactg cagcgaaagc gattttaaat
    17161 aaacaacatg gtccaaacac tgcgcaaaat gcagttgagg cagcattgca acgtgtgaat
    17221 actgcgaaaa atgcattgaa tggtgatgca aaattaattg cagctcaaaa cgcagcgaaa
    17281 caacatctag gtactttaac gcatatcact acagcacaac acaatgattt aacaaatcaa
    17341 atttcacaag ctacaaactt agctggtgtt gaatctgtta agcaaagtgc gaatagttta
    17401 gatggtgcaa tgggcaactt acaaaccgct atcaacgata aatctggaac attagcgagc
    17461 caaaacttcc tagatgctga tgagcaaaaa cgtaatgctt acaatcaagc tgtatcaaat
    17521 gccgaaacaa ttttaaataa acaaactgga ccgaatacag cgaaaactgc agtcgaacaa
    17581 gcacttaata atgttaatag tgcgaaacac gcattaaatg gtacgcaaaa cttaaataat
    17641 gcgaaacaag cagcgattac agcaatcaat ggatcatctg atttaaatca acatcaaaaa
    17701 gaggcattaa aagcacaagc taatggtgct caacgcgtat ctaatgcaca agatgtacaa
    17761 cgtaatgcga ctgaactgaa cacggcaatg ggcacattaa aacatgccat cgcagataag
    17821 acgactacgt tagcaagcag taaatatgtc aacgccgata gcactaaaca aaatgcttac
    17881 acaactaaag ttaccaatgc tgaacatatt attagcggta cgccaacggt tgttacaaca
    17941 ccttcagaag taacagctgc agctaatcaa gtaaacagcg cgaaacaaga attaaatggt
    18001 gacgaaagat tacgtgaagc aaaacaaaac gccaatactg ctattgatgc attaacacaa
    18061 ttaaatacac ctcaaaaagc taaattaaaa gaacaagtgg gacaagctaa tagattagaa
    18121 gacgtacaaa ctgttcaaac aaatggacaa gcattgaaca atgcaatgaa aggcttaaga
    18181 gatagtattg ctaacgaaac aacagtcaaa gctggtcaaa actatacaga cgcaagtccg
    18241 aataaccaat caacatataa tagcgctgtg tcaaatgcga aaggtatcat taatccaact
    18301 aacaatccga ctatggatgc tagtgcgatt acccaagctg caacacaagt gaataatgct
    18361 aaaaatggtt taaacggtgc tgaaaactta agaaatgcac aaaatactgc taagcaaaac
    18421 ttaaatacgt tatcacactt aactaataac caaaaatctg caatctcatc acaaattgat
    18481 cgtgcaggtc atgtgagtga ggtaacagca gctaaaaatg cagcaactga gttaaacacg
    18541 caaatgggca acttggaaca agctatccat gatcaaaata cagttaaaca aagtgttaaa
    18601 tttactgatg cagataaagc caaacgtgat gcgtatacaa atgcggtaag cagagcagaa
    18661 acaattctga acaaaacgca aggtgcaaat acgcctaaac aagatgtaga agcgtctatt
    18721 caaaatgttt caagtgctaa aaatgcattg aatggtgatc aaaacgttac aaatgcgaag
    18781 aatgcagcta aaaatgcatt aaataattta acttcaatta ataatgcaca aaaacgtgac
    18841 ttaacaacta aaattgatca agcaacaact gtagctggtg ttgaagcggt atctaataca
    18901 ggcacacaat tgaatacagc gatggctaag ttgcaaaatg gtattaatga taaagcgaat
    18961 actttagcga gcgaaaacta tcatgatgct gattcagata agaaaactgc ttatacgcaa
    19021 gccgttacga acgcagaaaa tattttaaat aaaaatagtg gatcaaattt agataaagct
    19081 gccgttgaaa acgcgttgtc acaagtaaca aatgcgaaag gtgccctaaa tggtaaccat
    19141 aatttagagc aagctaaatc aaatgcaaac actactataa acggacttca acatttaaca
    19201 actgcacaaa aagataaatt gaaacaacaa gtgcaacaag cacaaaatgt tgcaggtgta
    19261 gatactgtta aatcaagtgc caacacatta aatggtgcta tgggtacgtt aagaaatagc
    19321 attcaagaca atgcggctac gaaaaatggc caaaactatc ttgatgctac agaaagtaac
    19381 aaaacaaact ataacaatgc tgttgatagt gctaatagtg tcattaatgc aacaagcaat
    19441 ccaaatatgg atgctaatgc aattaaccaa atcgctacac aagtgacatc aacgaaaaat
    19501 gcattagatg gtacacataa tttaacgcaa gcgaaacaaa cagcaacaaa tgcgattgat
    19561 ggtgctgcta acttaaataa agcgcaaaaa gatgcgttaa aagcacaagt tacaagtgcg
    19621 caacgtgttg caaatgtaac aagtatccaa caaactgcaa atgaacttaa tacagctatg
    19681 ggtcaattac agcatggtat tgatgatgaa aatgcaacaa aacaaactca aaaatatcgt
    19741 gacgctgaac aaagtaagaa aactacttat gatcaagctg tagctgctgc gaaagcaatt
    19801 ttaaataaac aaactggttc aaattcagat aaagcagcag ttgaccgtgc attacaacaa
    19861 gtaacaagta cgaaagatgc attgaatggt gatgcgaaac tagcagaagc gaaagcggca
    19921 gctaaacaaa acttaagtac tttaaaccat attacaaatg cacaacgtac tgcgttagaa
    19981 ggtcaaatca atcaagcgac gactgttgat ggcgttaata ctgtaaaaac aaatgccaat
    20041 acattagacg gcgcaatgaa tagtttgcaa ggttcaatca atgataaaga tgcgacatta
    20101 agaaatcaaa attatcttga tgcggatgaa tcaaaacgaa atgcatatac tcaagctgtc
    20161 acagcggcag aaggcatttt aaataaacaa actggtggta acacatctaa agcagacgtt
    20221 gataatgcat taaatgcagt tacaagagcg aaaacagcgt taaatggtgc tgaaaactta
    20281 agaaatgcga agacaacagc aacaaatacg attaatggtt taccacactt aacacaatta
    20341 caaaaagaca acttgaagca tcaagttgaa caagcacaaa atgttgcagg tgtaaatggt
    20401 gttaaagata aaggtaatac attaaatact gccatgggtg cattacgtac aagtatccaa
    20461 aatgataata cgacgaaaac aagtcaaaat tatcttgatg catctgatag caacaaaaat
    20521 aattacaata ctgctgtaaa taatgcaaat ggtgtaatta atgctacgaa taatccaaat
    20581 atagatgcta atgcgattaa tggcatggca aatcaagtta atacaacaaa agctgcgtta
    20641 aatggtgcac aaaacttagc tcaagctaag acaaatgcaa cgaacacaat taacaacgca
    20701 catgacttaa accaaaaaca aaaagatgca ttgaaagcac aagttaacaa tgcacaacgt
    20761 gtatctgatg cgaataacgt tcaacatact gcaactgaat tgaacggcgc aatgacagca
    20821 cttaaagcag ctattgctga taaagaaaga acaaaagcaa gcggtaatta tgtcaatgct
    20881 gatcaagaaa aacgtcaagc gtatgattca aaagtgacga acgctgaaaa tatcattaat
    20941 ggtacaccga atgcgacatt aacagtcaat gacgtaaata gtgcgacttc acaagtaaat
    21001 gcggctaaaa cagcattaaa tggtgataac aacttacgtg ttgcgaaaga gcatgccaac
    21061 aatacaattg atggcttagc gcaattgaat aatgcacaaa aagcaaaatt aaaagaacaa
    21121 gttcaaagtg caactacatt agatggcgtt caaactgtta aaaatagttc tcaaacgttg
    21181 aatacagtga tgaaaggctt aagagatagt attgcgaatg aagcaacaat taaagcaggt
    21241 caaaactaca ctgacgcaag tctgaataat cgtaacgagt acgacagcgc agttactgca
    21301 gcaaaagcaa tcattaatca aacatcgaac ccaacgatgg aagcaaatac tattacgcaa
    21361 gcaacatcac aagtgacaac taaagaacag gcattaaatg gtgcgcaaaa cttagctcaa
    21421 gctaagacaa ctgcgaaaaa caacttgaat aacttaacat caattaacaa tgcacaaaaa
    21481 gatgcgttaa cgcgtagcat agatggtgca acaacagtag ctggtgtaaa tcaagaaact
    21541 gccaaagcaa ctgaattaaa taacgcaatg cacagtttac aaaatggtat caatgatgag
    21601 acacaaacta aacaaactca gaaataccta gatgcagagc caagtaagaa atcagcttat
    21661 gatcaagctg taaatgcagc gaaagcaatt ttaacaaaag ctagtggtca aaatgtagac
    21721 aaagctgcag ttgaacaagc attacaaaat gtgaacagta cgaagacggc gttgaacggt
    21781 gatgcgaaat taaatgaagc taaagccgct gcgaaacaaa cgttaggtac attaacacat
    21841 attaataatg cacaacgtac agcgttagat aatgaaatta cacaagcaac aaatgttgaa
    21901 ggtgttaata cagttaaagc caaagcgcaa caattagatg gtgctatggg tcaattagaa
    21961 acatcaattc gtgataaaga cacgacgtta caaagtcaaa attatcaaga tgctgatgat
    22021 gctaaacgaa ctgcttattc tcaagcagta aatgcagcag caacaatttt aaataaaaca
    22081 tctggtggca atacacctaa agcagatgtt gaaagagcaa tgcaaacagt tacacaagca
    22141 aatactgcat taaacggtat tcaaaactta gaacgtgcga aacaggctgc taacacagcg
    22201 attacaaatg cttcggactt aaatacaaaa caaaaagaag cattaaaagc acaagtaaca
    22261 agtgcaggac gcgtatctgt agcaaatggt gttgaacata ctgttactga attaaatact
    22321 gcgatgacag ctttaaaacg tgccattgct gataaagctg acacaaaagc tagtggtaac
    22381 tatgtcaatg ctgatgcgaa taaacgccaa gcatatgatg aaaaagtgac agctgcagaa
    22441 aatatcgtta gtggtacacc aacaccaacg ttaacaccat cagatgttac aaacgcagca
    22501 acgcaagtaa cgaatgcgaa gacgcagtta aacggtaatc ataatttaga agtagcgaaa
    22561 caaaatgcga acacagcaat tgatggttta acttctttaa atggtccaca aaaagcaaaa
    22621 cttaaagaac aagtgggtca agcgacgacg ttgccaaatg ttcaaactgt tcgtgataat
    22681 gcacaaacat taaacactgc aatgaaaggt ctacgagata gcattgcgaa tgaagcaacg
    22741 attaaagcag gtcaaaacta cacagatgca agtcctaata atcgtagtga atacgatagt
    22801 gcagtcactg cagcaaaagc aatcattggt caaacaacta gtccaacaat gaatgctcaa
    22861 gaaatcaatc aagcgaaaga ccaagtgaca gctaaacaac aagcgttaaa cggtcaagaa
    22921 aacttaagaa ctgcgcaaac aaatgcgaag caacatttaa atggcttaag tgacttaact
    22981 gacgctcaaa aagatgcagt gaaacgtcaa atcgagggtg caacgcatgt taatgaagta
    23041 acacaagcac aaaataatgc ggacgcatta aatacagcta tgacgaactt gaaaaatggt
    23101 attcaagatc aaaatacgat taagcaaggt gttaacttca ctgatgcaga tgaagcgaaa
    23161 cgtaatgcat atacaaatgc agtgacgcaa gctgaacaaa ttttaaataa agcacaaggt
    23221 ccaaatactg caaaagacgg tgtcgaaact gcgttacaaa atgtacaacg tgctaaaaac
    23281 gatttgaacg gtaatcaaaa tgttgcgaat gctaagacaa ctgcgaaaaa tgcattaaat
    23341 aatttaacat caattaataa tgcacaaaaa gaagcgttga aatcacaaat tgatagtgcg
    23401 acaacagttg caggtgtaaa tcaagtgtct acaacggcat ctgaattaaa tacagcaatg
    23461 agcaacttac aaaatggtat taatgatgaa gcagctacaa aagcagctca gaaatatact
    23521 gatgcagata gtgataaaca aactgcatac aatgatgctg taacagcagc taaaacgtta
    23581 ttagataaaa cagctggcac aaatgacaat aaagcagccg ttgaacaagc attacaacgt
    23641 gtgaatactg ctaaaacagc attaaatggt gacgcgcgac taaatgaagc gaagaacaca
    23701 gctaaacaac aattagcgac aatgtcacat ttaactgatg ctcaaaaagg aaacttaaca
    23761 gaacaaattg aacgcggtac gactgttgct ggtgttcaag gtattcaagc taatgccggt
    23821 actttagatc aagcaatgaa tcaattaaga caaagtattg tatctaaaga tgcgactaaa
    23881 gcgagcgaag attatcatga tgcgaataca gatttacaaa atgcatacaa ccatgcagta
    23941 tctgaagctg aaggtattat cagcgcatca aataatcctg aaatgaatcc agatacaatt
    24001 aatcaaaaag cgagccaagt gaacagtgcg aattctgcat tgaacggtga tgaaaaatta
    24061 gcagaagcaa aacaaactgc gaaatcagat atcggtcgtt tatcagactt aaacaacgca
    24121 caacaaactt ccgcaactgc tgaagtagat catgcaccaa accttgcagc tgtaacagca
    24181 gctaaaaata aagcgacatc gttaaacaca gcgatgggta atttgaaaca tgcacttgct
    24241 gaaaaggata atacgaagcg aagtgtcaat tacacagatg cggatcaacc aaaacaacaa
    24301 gcgtatgaca ctgcagttac acaagcagaa ggaattacta atgccaatgg cagcaacgcg
    24361 aatgaaacac aagttcaagc agcgcttaac caattgaatc aagctaaaaa tgacttgaat
    24421 ggtgataata aagttgctca agctaaagaa gcagcgaaac atgcattagc ttcatatagt
    24481 aacttgaata atgcgcaatc aactgcagca actagtcaaa ttgacaatgc aacgacagta
    24541 gcaggcgtaa ctactgcaca aaatactgct aatgagttaa atacagcaat gggtcaactt
    24601 caaaatggta ttaatgacca aaacactgtt aaacaacaag tgaactttac agatgctgac
    24661 caaggtaaga aagatgctta cacaaatgct gttacgaatg ctcaaggtat tttagataaa
    24721 gcaaacggtc aaaatatgac gaaagcacaa gttgaagcgg cattaaatca agtaacgact
    24781 gctaaaaatg ctttaaacgg tgatgcaaat gtaagacaag caaaatcaga tgcgaaagca
    24841 aacttaggta cattaacaca cttaaataat gcacaaaaac aagatttaac atcacaaatt
    24901 gaaggtgtaa caacagtcaa cggtgtaaat ggtgttaaaa cgaaagcaca agacttagat
    24961 ggcgcaatgc aacgattaga gtcagcaatc gcaaataaag atcaaactaa agcaagcgaa
    25021 aactacatcg acgcagatcc aactaacaaa acagcatttg ataatgctat cacacaagct
    25081 gaatcttact taaataaaga tcatggtgcg aataaagata agcaagctgt tgaacaagca
    25141 attcaaagtg taacatctac tgaaaatgct ttgaacggtg acgcgaactt acaacgcgct
    25201 aaaactgaag ctacacaagc tatcgataac ttgacacatt tgaacacacc acaaaaaaca
    25261 gcattaaaac aacaagtaaa cgctgcacaa cgtgtatcag gtgtaactga tttgaaaaat
    25321 agtgctacat cacttaatag tgcgatggat caattaaaac aagcaattgc tgatcatgac
    25381 acaattgtag ctggtggtaa ttacactaac gcgagtcctg acaagcaagg tgcttacact
    25441 gatgcatata atgctgcgaa aaacattgta aatggttcac ctaatgtgat tacaaatgca
    25501 gcagatgtta cagcagcaac acaacgtgtc aataatgctg aaacaggttt aaacggtgat
    25561 acaaacttag caactgcgaa gcaacaagct aaagatgcat tacgtcaaat gacacattta
    25621 tctaatgcac aaaaacaaag tattactggt caaattgata gcgcgacaca agtaactggc
    25681 gttcaaagtg tgaaagacaa tgcaacaaat cttgataatg caatgaatca acttcgaaat
    25741 agtattgcga ataaagatga agtaaaagcg agtcaaccat atgttgatgg agatacggat
    25801 aaacaaaatg catacaacac agctgttaca agtgctgaaa atatcattaa tgcaacgagt
    25861 cagccgacac ttgatccatc tgcagtaaca caagcagcta atcaagtgaa cactaacaaa
    25921 acagcgctta atggtgcaca aaacttagca aataaaaagc aagaatcaac tgctaacatc
    25981 aaccaattaa gtcatttaaa taatgctcaa aagcaagatt taaatacgca agtgacaaat
    26041 gcaccaaata ttagcacagt aaatcaagtg aaaactaaag ctgaacaatt agatcaagca
    26101 atggaacgtt taatcaacgg aatccaagac aaagatcaag tgaaacaaag tgttaacttt
    26161 acagatgcag atccagaaaa acaaacagca tacaacaatg cggtaactgc tgctgaaaat
    26221 attattaatc aagcaaatgg tactaatgag aaccaatcac aagttgaagc agcactttca
    26281 actgtaacaa ctactaaaca agcgttgaat ggtgatagaa aagtaacaga tgctaaaaac
    26341 aatgcaaacc aaacattatc tacgttagat aacttaaaca atgcacaaaa aggtgctgtt
    26401 actggaaaca tcaatcaagc gcacactgta gctgaagtaa cgcaagccat tcaaacagct
    26461 caggaactca atacagcgat gggtaacttg aaaaatagct tgaatgataa agacactaca
    26521 cttggcagtc aaaactttgc agatgcagat ccagagaaga aaaatgcata caatgaagcg
    26581 gttcgtaatg ctgaaaatat tttaaataaa tctacaggta cgaacgtgcc taaagatcaa
    26641 gttgaagcag ctatgaatca agtgaatacg acgaaagcag cgcttaatgg ttctcaaaat
    26701 cttgaaaaag cgaagcaaca tgcgaataca gcaattgacg gtttaagcca tttaacaaat
    26761 gcacaaaaag atgcattaaa acaattggta caacaatcga ctactgttgc agaagcacaa
    26821 ggtaatgaac aaaaagcaaa caatgttgat gcagcaatgg acaaattacg tcaaagtatt
    26881 gcagataatg cgacaacaaa acaaaaccaa aattatactg atgcaagtcc gaataaaaag
    26941 gatgcgtaca ataatgctgt cacaactgca caaggtatta ttgatcaaac tacaagtcca
    27001 actttagatc cgactgttat caatcaagct gctggacaag taagcacaac taaaaatgca
    27061 ttaaatggta atgaaaacct agaagcagcg aaacaacaag cgacacaatc attaggttca
    27121 ttagacaact taaataatgc gcaaaaacaa gctgttacta atcaaattaa tggcgcgcat
    27181 actgttgatg aagcaaatca aattaaacaa aatgcgcaaa acttaaatac agcgatgggt
    27241 aacttgaaac aagcgattgc tgacaaagat gctacgaaag caacagtaaa cttcactgat
    27301 gcagatcaag caaaacaaca agcatataac actgctgtta caaatgctga aaacatcatt
    27361 tcaaaagcta atggtggtaa tgcaacacaa actgaagttg aacaagcaat ccaacaagtt
    27421 aatactgcaa aacaagcatt aaatggtaat gccaacgttc aacatgcgaa agatgaagca
    27481 acagcattaa taaataactc taatgatctt aaccaagcac agaaagatgc attaaaacaa
    27541 caagtacaaa atgcaacaac tgttgctggt gtaaacaatg ttaaacaaac agcgcaagag
    27601 ttaaacaatg cgatgacaca attaaaacaa ggcattgcag ataaagagca aacaaaagct
    27661 gatggtaact ttgtcaatgc agatcctgat aagcaaaatg catataaaca agcagttgcg
    27721 aaagctgaag cattaattag tggtacgcct gatgttgtcg ttacacctag cgaaattact
    27781 gcagcgttaa ataaagttac gcaagctaaa aatgatttaa atggtaatac gaacttagca
    27841 acggcgaaac aaaatgttca acatgctatt gatcaattgc caaacttgaa tcaagcgcaa
    27901 cgtgatgaat acagtaaaca aatcacgcaa gcaacgcttg taccaaacgt caatgctatt
    27961 caacaagcgg caacaacgct taatgacgcg atgacacaat tgaaacaagg tatcgcgaat
    28021 aaagcacaaa ttaaaggtag cgaaaactat catgatgctg atactgataa gcaaacagca
    28081 tatgacaatg cagtaactaa agcagaagaa ttgttaaaac aaacaacaaa tccaacaatg
    28141 gatccaaata caattcaaca agcattaact aaagtgaatg atacaaatca agcacttaac
    28201 ggtaatcaaa aattagctga tgccaaacaa gctgctaaga caaatcttgg tacattagat
    28261 catttaaatg atgctcaaaa acaagcgtta acaacacaag ttgaacaagc accagatatt
    28321 gcaacagtta ataatgttaa gcaaaatgct caaaatctga ataatgctat gactaactta
    28381 agtaatgcat tacaagataa aactgagaca ttaaatagca ttaactttac tgatgcagat
    28441 aaagctaaga aagatgctta tactaatgcg gttgcacaag cagaaggtat tttatctaaa
    28501 gcaaatggca gcaatgcaag tcaaactgaa gtggaacaag cgatgcaacg tgtgaacgca
    28561 gcgaaacaag cattgaatgg taatgacaat gtacaccgtg caaaagatgc agcgaaacaa
    28621 gttattacaa atgcgaatga tttaaatcaa gcgcaaaaag atgcgttaaa acaacaagtt
    28681 gatgctgcgc aaactgttgc aaatgtaaat acaaataagc aaacagcaca agatttaaat
    28741 caagcaatga cacaattgaa acaaggtgtt gcagataaag accaaactaa agcaaatggt
    28801 aactttgtca atgcagatac tgaaaagcaa aatgcttaca acaatgcggt agcacatgct
    28861 gaacaaatca ttagcggtac accaaatgca aacgtggatc cacaacaagt ggctcaagcg
    28921 ttacaacaag tgactgatgc taaaaatggc ttaaacggta accataactt acaagttgct
    28981 aaagacaatg cgaatactgc cattgatcag ttaccaaact taaatcaacc acaaaaaaca
    29041 gcattaaaag accaagtgac acatgcagaa cttgttacag gtgttaatgc tattaagcaa
    29101 aatgctgatg cattaaataa tgcaatgggt acgttgaaac aacaaattca agcaaacagt
    29161 caagtaccac aatcagttga ctttacacaa gcggatcaag acaaacaaca agcatataac
    29221 aatgcagcta accaagcgca acaaattgca aatggcacac caacacctgt attgacgcct
    29281 gacgcagtaa cacaagcagt tactacaatg aatcaagcga aagatgcatt aaacggtgat
    29341 gaaaaattag cacaagcgaa acaagatgct ttagcaaatc ttgatacgtt acgtgactta
    29401 aatcaaccac aacgagatgc gttacgtaat caaatcaacc aagcacaagc gttagctaca
    29461 gttgaacaaa ctaaacaaaa tgcacaaaat gtgaatacag caatgagtaa cttgaaacaa
    29521 ggtattgcaa ataaagatac tgtcaaagca agtgaaaact accacgatgc tgatgccgat
    29581 aagcaaacag catatacaaa tgcagtgtct caagcggaag gtattatcaa tcaaacgaca
    29641 aatccaacgc ttaacccaga tgatattact cgtgtattaa ctcaagtgac tgatgctaaa
    29701 aatggcttaa acggtgaagc taaattggca actgaaaaac aaaatgctaa agatgctgtc
    29761 aatgccatga cgcatttaaa cgatgcacaa aaacaagcat tgaacggtca aatcgatcaa
    29821 tcacctgaaa ttgcaacagt aactcaagtt aaacaaacag caacgagcct agatcaagca
    29881 atgaatcaat tatcacaagc tattaatgat aagactcaaa cattagcgga tggtaattat
    29941 ttaaatgcag atcctgacaa acaaaatgcg tataaacagg ccgtagcaaa agctgaagca
    30001 ttattgaata aacaaagtgg tactaatgaa gtacaagcac aagttgaaag catcactaat
    30061 gaagtgaacg cagcgaaaca agcattaaat ggtaatgata atttagcgaa tgcgaagcaa
    30121 caagcgaaac aacaactggc gaatttaaca cacttaaatg atgcacaaaa acaatcattt
    30181 gaaagtcaaa ttacacaagc gccacttgtt acagatgtca ctacaattaa tcaaaaagcg
    30241 caagcgttag atcatgcgat ggaattatta agaaatagta ttgcagataa tcaagcgaca
    30301 ttagcatctg aagattatca tgatgcagct gcgcaaagac aaaatgacta taatcaagct
    30361 gtaacagctg ctaataatat cattaatcaa acaacgtcac ctacgatgaa tccagatgat
    30421 gttaatcgtg caacaactca agtaaataac acgaaagttg cattagatgg tgatgaaaac
    30481 cttgcagcag ctaagcaaca agcgaacaat agacttgatc aattagatca tttaaataat
    30541 gcgcaaaagc aacagttaca atcacaaatt acgcaatcat ctgatattgc tgcagttaat
    30601 ggtcacaagc aaactgcaga atctttaaat accgcaatgg gtaacttaat caatgcgatt
    30661 gcagatcatc aagccgttga acaacgtggt aacttcatca atgctgatac tgataaacaa
    30721 actgcttata ctacagcggt aaatgaagca gaagccatga ttaacaaaca aactggtcaa
    30781 aatgctaacc aaacagaagt agaacaagct attactaaag ttcaaactac acttcaagcg
    30841 ttaaatggag atcataattt acaagttgct aaaacaaatg cgacgcaagc aattgatgct
    30901 ttaacaagct taaatgatcc tcaaaaaaca gcgttaaaag accaagttac tgctgcaact
    30961 ttagtaactg cagttcatca aattgaacaa aatgcgaata cgcttaacca agcaatgcat
    31021 ggcttaagag aaagtattca agataacgca gcaactaaag caaatagcaa atatatcaac
    31081 gaagatcaac cagagcaaca aaactacgat caagcagtgc aagctgcaaa taacattatc
    31141 aatgaacaaa cagcgacatt agataacaat gtgattaatc aagcagcgac aactgtgaat
    31201 acaacgaaag cagcattaca tggtgatgtg aagttacaaa atgataaaga tcatgcgaaa
    31261 caagcggtta gtcaattagc acatttaaac aatgctcaaa aacatatgga agatacgtta
    31321 attgatagtg aaacaactag aacagccgtt aaccatgatt tgactgaagc acaagcatta
    31381 gatcaactaa tggatgcatt acaacaaagt attgctgaca aagatgcaac acgtgcgagc
    31441 agtgcatatg tcaatgcaga accgaataaa aaacaagcat atgatgaagc agttcaaaat
    31501 gctgagtcta tcattgcagg attaaataat ccaactatca ataaaggtaa tgtaacaagt
    31561 gcgacacaag ctgtaacatc atctaaaaat gcattagacg gtgttgaacg attagcgcaa
    31621 gataagcaaa cagctggaaa ttctctaaat catttagatc aattaacacc agctcaacaa
    31681 caagcgctag aaaatcaaat taataatgca acaactcgtg ataaagtggc tgaaatcatt
    31741 gcacaagcgc aagcattaaa tgaagcgatg aaagcattaa aagaaagtat taaagatcaa
    31801 ccacaaacag aagcaagtag taaatttatt aacgaggatc aagcgcaaaa agatgcttat
    31861 acgcaagcag tacaacatgc aaaagatttg ataaacaaaa ctactgatcc tacattagtt
    31921 aaatcagtaa ttgatcaagc aacacaagca gtgaatgacg ctaaaaacaa cttacatggt
    31981 gatcaaaaat tagctcaaga taagcaacgt gcaacagaaa cgttaaataa cttgtctaac
    32041 ttgaatacac cacaacgtca agcactagaa aatcaaatca ataatgcagc aactcgtggc
    32101 gaagtagcac aaaaattaac tgaggcacaa gcacttaacc aagcaatgga agcattacgt
    32161 aatagcattc aagatcaaca gcaaacagaa gcgggtagca agtttatcaa tgaagataag
    32221 ccacaaaaag atgcatacca agcagcagta caacatgcca aagatttgat taatcaaact
    32281 agcaatccaa cgcttgataa agcacaagtt gaacaattga cacaagctgt taaccaagct
    32341 aaagataact tacatggtga tcaaaaactt gcagacgata aacaacatgc ggttactgat
    32401 ttaaatcaat taaatggttt gaacaatccg caacgtcaag cacttgaaag tcaaataaac
    32461 aacgcagcaa ctcgtgatga agtagcacaa aaattagctg aagcaaaagc acttaatcaa
    32521 gcaatggaag cattacgtaa tagtattcaa gatcaacaac aaacagaatc tggtagcaag
    32581 tttatcaatg aagataagcc acaaaaagat gcataccaag cagcagtaca acatgccaaa
    32641 gatttaatta accaaacagg taatccaaca ctcgataaat cacaagtgga acaattaaca
    32701 caagcagtag caactgcaaa agataaccta catgttgatc aaaaacttgt tcgtgatcaa
    32761 caacaagcag taacgactgt taatgcatta ccaaacttaa atcatgcgca acaacaagct
    32821 ttaactgatg ctataaatgc agcgcctaca agaacagagg ttgcacaaca tgttcaaact
    32881 gctactgaac ttgatcacgc aatggaaaca ttgaaaaata aagttgatca agtgaacaca
    32941 gataaggctc aaccaaatta cactgaagca tcaacggata aaaaagatgc agtagatcaa
    33001 gcattacaag ctgctcaaag cattacagat ccaactaatg gttcaaatgc aaataaatat
    33061 gctgtagaac aagcattaac gaagctgcaa ggaaaagaaa atgagttaaa tggtaatgag
    33121 agagtcgctg aagctaaaac acaagcgaaa caaactattg accaattaac acatttaaat
    33181 gctgatcaaa ttgcaactgc taaacaaaac attgatcaag cgacgaaact tcaaccaatc
    33241 gctgaattag tagatcaagc aacgcaattg aatcaatcta tggatcaatt gcaacaagca
    33301 gttaatgaac atgctaacgt tgagcaaact gtagattaca cgcaagcaga tttagataag
    33361 caaaatgctt ataaacaagc tattgcagaa gctgaaaatg tattaaaaca aaatgcgaat
    33421 aagcaacaag tggatcaagc acttcaaaac attttaaatg caaaacaagc attaaatggt
    33481 gatgaacgtg tagcacttgc taaaacaaat ggtaaacatg acattgacca attaaatgca
    33541 ttaaacaatg ctcaacaaga tggatttaaa ggtcgcatcg atcaatcaaa cgatttaaat
    33601 caaatccaac aaattgtaga tgaggctaag gcactaaatc gtgcaatgaa tcaattgtct
    33661 caagaaatca ctggcaatga aggacgtacg aaaggtagta cgaactatgt caatgcagat
    33721 acacaagtca aacgcgtata cgatgaagca gttgataaag cgaaggaagc actaaataaa
    33781 gcgacaggtc aaaacttaac tgctgaagaa gttatcaaat tgaacgatgc ggtaacggca
    33841 gcgaaacagg cattaaatgg tgaagaaaga cttaataacc gtaagtctga agcattacaa
    33901 agattggatc aattaacaca tctaaacaat gctcaaagac aattagcaat tcaacaaatt
    33961 aataatgctg aaacgctaaa taaagcatct cgagcaatta atagagctgt tcaattagat
    34021 gatgcaatgg gtgcagtaca acagtatatt gatgaacatc atcttgatgt tattagcagt
    34081 acaaattaca tcaatgcaga cgataacttg aaagcaaatt atgacaatgc cattacaaat
    34141 gcagcacatg agttagataa agttcaaggt agtgcaatcg cgaaagctga ggcagaacaa
    34201 ttaaaacaac atattatcga tgctcaaaaa gccttaaatg gtgatcaaaa tcttgcaacg
    34261 gctaaagata aagccaatgc atttgttgat acattaaatg gtttaaatca acaacaacaa
    34321 gatcttgcac atcaagcaat taataatgcc gatactgtaa caggtatagc gaatattatt
    34381 aatgatcaaa tcgacttgaa taacgcgatg gagacattga aacacttagt tgacaatgaa
    34441 attccaactg cagaacaaac tgtcaattac caaaatgctg acgacgttgc aaaatcaaac
    34501 tttgatgatg caaaacgtct ggcaaatgca ttaattaata gtgataacac aaatgtgaat
    34561 gatatcaacg gcgcaattca agcagtcaaa gatgtaatcc aaaatcttaa cggtgagcaa
    34621 cgtctacaag aagctaagga caaagcaatt caaaatgtta acaaagtatt agcagataaa
    34681 ttgaaagaaa tcgaagcttc aaacgcgacg gatcaagaca agcttattgc gaaaaataaa
    34741 gcagaagaat tggcaaacag catcattaat aacattaata aagcaacaag taatcaggat
    34801 gtatctcgag ttcaaacagc tggcaaccaa gcgattgaac aagtgcatgc caatgaaata
    34861 ccaaaagcta aaattgatgc aaataaagat gttgataagc aagttcaagc attaattgac
    34921 gaaattgatc gaaatccaaa tctaacagat aaagaaaaac aagcgcttaa agatcgaata
    34981 aatcaaatcc tgcaacaagg tcataacggc attaacaatg cgatgactaa agaagaaatt
    35041 gagcatgcca aagcgcaact tgcacaagca ttacaagaca ttaaagattt agtgaaagct
    35101 aaagaaaatg cgaaacaaga tgttgataag caagttcaag cattaattga cgaaattgat
    35161 cgaaatccaa atctaacaga caaagaaaaa caagcgctta aagatcgaat aaatcaaatc
    35221 ttgcaacaag gtcataacgg cattaacaat gcgatgacta aagaagaaat tgaacaagcc
    35281 aaagcgcaac ttgcacaagc gttacaagac attaaagatt tagtgaaagc taaagaaaat
    35341 gcgaaacaag atgttgataa gcaagttcaa gcattaattg acgaaattga tcgaaatcca
    35401 aatctaacag ataaggaaaa acaagcactt aaagatcgaa taaatcaaat cttgcaacaa
    35461 ggtcataatg gcattaacaa tgcgatgact aaagaagaaa ttgaacaagc caaagcgcag
    35521 cttgcacaag cattacaaga cattaaagat ttagtgaaag ctaaagaaga tgcgaaaaat
    35581 gcaattaaag cattagcgaa tgagaagcgt gatcaaatca attcaaaccc tgatttaaca
    35641 cctgaacaaa aagcaaaagc gctcaaagaa attgacgaag ctgaaaaacg agcactagaa
    35701 aacattgaaa atgctcaaac taaagatcaa ttaaataaag gattaaattt aggattagat
    35761 gatattagaa atacacatgt atgggaagtt gatgcacaac ctgctgtaaa tgaaattttt
    35821 gatgcaactc ctgaacaaat ccttgtaaat ggtgaactca ttgtacatcg tgatgacatc
    35881 atcactgaac aagatattca tgcacacatc aacttaattg atcaacttac agcggaaatt
    35941 attgatacac cgtcaactgc aacaatttct gatagtttaa cagcaaaagt tgaagtaaca
    36001 ttgcttgatg gatcaaaagt gattgttagt gttcctgtga aagtagtaga aaaagaattg
    36061 acagtagtca aacagcaggc gattgaatca attgaaaatg cggcacaaca aaagattaat
    36121 gaaatcaata atcacgcaac attaacacct gaacaaaaag aagctgcgat tgcggaagtt
    36181 aataagctta aacaacaagc tattgagcaa attaacaacg cagcagatgt tcatacggtg
    36241 gaagaagttc aacaccaaga acaggcgcat attgaacaat tcaatccaga tcaatttaca
    36301 attgaccaag ccaaatcaaa tgccattaaa tcaatttctg atgctatcca acatatgatt
    36361 gatgaaatta atgctagtaa ggatttaact gataaagaga agcaagaagc tatatctaaa
    36421 ttaaatcagt taaaagatca atcaattcag gcgattcaac gtgcgcaaag catcgatgaa
    36481 attgcgcaac aattagagca atttaaagcg caactgaaag cggcaaatcc atttgctaaa
    36541 gaattggaaa accgtaaaaa atcagcgatt tctaaaatta aagatatttc tacagatgaa
    36601 attaatcgca ttagaaatag cacaattgga actgctgaag aaagacaagc agcaatgaat
    36661 cgaattaacg aaattgtgct tgaaacaatc agagatatta ataatgcgca tacgccacaa
    36721 caagttgagg ctgcattgaa taatggtatc gcacgaattt tagcagtaca aattgtaaca
    36781 tctgatcata gcaaaccttc atcaaattca gatggtcaat ctaatagtca tttacacgtt
    36841 ggttatggca ctgtaaatca tccatataac agtacgacta ttggacataa gaagaaattg
    36901 gatcaagatg atgaaatcga tccacttcat atgcgtcatt tcggtgaccg aattggtaat
    36961 gttatcaaaa acgcacttgg cgttgttggt atttccggat tacttgctag cttctggttc
    37021 ttcattgcga aacgtcgtcg taaagaggat gaagaggaag aattggaaat tagagataat
    37081 agcaaagaca agaagaaagg ttcagtagaa ggtactaaac atttaccgct tttatttgcg
    37141 aaacgtcgca gaaaagaaga tgaagaagat gctattgttg aagaaaaaga ttcgctaaac
    37201 aatgacgagt cactcgataa agttaaacat acaccgttct tcttacctaa acgtcgcaga
    37261 aaagaagatg aagaagatgt ggaagttaca aatgaaaaca cagatgaaaa agtgttgcaa
    37321 gataacgaac attcaccaat cttaattgca aaacgccgtc gcgataaaga ggaagatgtt
    37381 gaaacaacaa gtagtattga ttctaaagat gaggacgtac ctgtattatt ggctaaaaag
    37441 aaaaatcaaa aagataacca atccaaaggg aaaaagtcgg catcaaaaaa accttctaaa
    37501 aaggtagcag ctaaaaagaa gaaaaagaaa tctaaaaaaa ataaaaaata atttgtttct
    37561 tcgataaaaa gaggagcacc gattgaaatt ttgtcagtcg gtgctccttt tatttgttct
    37621 ttttaattaa tttgtacaat gcttgctgtg cgtgttgatt cgcttctttg ttttgttctc
    37681 tcggaatcca tttaacaaat aataaatcaa aatcttgttc aaatatttcc aattgatcaa
    37741 aataaggttt aaattttgca tttttaacat aaccagcgtt aacactatcc gcaattaact
    37801 tagagtcagt gtacaacaac gcattgctaa cgtttaattc acgggcgtgt ttcaatgcat
    37861 agatacaagc agcccattct gcagtgtgat tatccatttc gcctaactca tgtgtatatg
    37921 taaaatgctg ctcatcttct ttgattacaa cggcacacgt acttatgcct ggatttcctt
    37981 tcgtcgcggc atcaaaattt attttcgcca taataaacct actttctatt caatacttaa
    38041 ttaaagttac tattactgta atataaaata cattaaacaa tctattaaaa aacacgcatc
    38101 acttaaataa gtaacgcgtg tcgtaaaata agctatgaat ttgaggcgtt atctgattca
    38161 atttcagttg tagatatttt cctaattgaa aagcactacg ttactttttc tgaaaaggtc
    38221 tcaaataaac ttacgaaaac tattatttaa tagccttgat ctaatttatc aatcttacct
    38281 ttacgataaa atgatttagc aatatatcca aatggtacat tgataactgt tgaagctaat
    38341 tttagtacgt acgttgtaat aaatatttca aatacaactg taccaggtaa acttccgata
    38401 aatgcgatag ctacaaataa agctgtatca attattgagc ttaaaaatgt actaccgtat
    38461 gcacggatga aaaacgtttt atcagaacta aatacttttt taattagtga aaagataaat
    38521 acatcaatat gttgaccaat aatatatgcg acaattgagc ctaaagctat gcgtggcaca
    38581 acatcaaaga ttgcgtgtaa tgctttttgt gccatatctt ctggtgcagg aataaaatgt
    38641 aatgacaatt gcataacaat aatcataatt aatgttgatg aaaagcctaa ccaaactgct
    38701 ctttttgcaa ctttacgccc ataaatatcg tttaatatat ctgttgctaa ataaatagaa
    38761 gcgaacatga cattacctaa agttgctgaa ataccaaaga tttctacagt tttaatcact
    38821 tgtatgttgg caataattgt gccaattgca acccatgcaa ttaaaccttg tttaccaaaa
    38881 aagcgataca taagtaccat aagcacgaac gttgcaataa acgtaactag tcctaaaatt
    38941 tcattataca tattaaaatg tcctcctaaa ttttgatcat gcgggtgttt agaaaccgct
    39001 caataaataa cttactcatt ttactgaaaa atcgtttgcg atgcaatagc atttgaatat
    39061 aaatacattt tacgtataga attgtaaaag gtttcattca aatcttaggg tcaaaaatgt
    39121 tataatattt ttatgtcaaa tttaaaacag taacacttat ttacaaggtt gcaatatttt
    39181 gaagtaataa aggaagtgtc gcgtatttta actttttcag agcaaaatgc actcgcgaaa
    39241 atagatgatt taatgaatac ttattgcaat caatgtccga tcaaaactcg tctgcgtaaa
    39301 ttagagggga aaacgaaggc gcatcatttt tgtatcaatg agtgttcaat agggaaagaa
    39361 ataaaacaat taggaaatga acttcaatag gaggaagtca aatgcaaatt atatctatat
    39421 cagaaacacc gaaccacaac acaatgaaga ttacacttag tgaaagcaga gaaggtatga
    39481 catcagatac gtatactaaa gttgatgatt cacagccagc ttttattaat gacatcttaa
    39541 aggtagaagg tgttaaatca attttccatg ttatggactt tatttcagta gataaagaaa
    39601 atgacgcaaa ttgggaaaca gtattgccaa aagtagaggc tgtattcgaa taaatttttc
    39661 atcaactagt attcgggggg aataaagtat atggaaattt tacgtataga gccaacgcca
    39721 agtccaaata caatgaaagt tgttttgtca tatacaagag aagacaagtt gtctaatact
    39781 tataaaaaag tagaagaaaa tcaaccaaga tttataaatc agttgttatc tatagatggt
    39841 atcacttcca tttttcatgt catgaacttc ttagctgttg ataaggcacc aaaagctgat
    39901 tgggaagtca tattacctga tattaaagct gttttttctg gtgaaagtca ggtaatggaa
    39961 actggaaaag acccacaaat tgacaatcat tttggtgaaa ttaaagctga actattaact
    40021 tttaagggta taccgtatca aattaaacta acttctgctg accaagaatt aagagaacaa
    40081 ttaccacaaa catatgttga tcatatgact caagcacaaa cagaacatga caatattgtt
    40141 tttatgcgta aatggctaga tttaggaaat cgctatggaa atattgaaga agtaatggat
    40201 ggtgtcctag aagaagtact agttacctat ccagaatcac agttacctgt attggtaaaa
    40261 catgctttag aagaaaatca cgcaactaat aattatcatt tctatcgaca tgtctctttg
    40321 gatgaatatc atgcgactga taattggaag actcgattac gaatgttaaa ccattttcca
    40381 aagccgactt ttgaagatat accgctgctt gatttagctt tatctgatga aaaagtaccg
    40441 gttagacgtc aagcgattgt actattaggc atgattgaaa gtaaagaaat tttaccgtat
    40501 ttatataagg ggcttcgtga taaaagtcct gctgtaagaa gaactgcagg ggattgcatt
    40561 agcgatttag ggtatccaga ggcactacca gaaatggtgc tactattaga tgatccacag
    40621 aaaatcgtta ggtggcgtgc tgctatgttt atctttgatg aaggtaatgc agagcagctt
    40681 cccgcactta aagcccatat taatgacaat gcgtttgaag ttaaattaca aattgaaatg
    40741 gccatatctc gtattgaaaa cggagatcaa gcacttggtt cagtatggaa acaaatgacg
    40801 aacagaacaa tttaattgga ggaattaaat atgaatgcat atgatgctta tatgaaagaa
    40861 attgcgcaac aaatgcgtgg cgaattaact caaaatggtt ttacaagttt agaaacgagc
    40921 gaagcagtat cagagtatat gaaccaagta aatgctgatg acactacttt tgtagttatt
    40981 aactctacat gcggctgtgc agctggatta gcaagaccag ctgcagtagc agttgcaaca
    41041 caaaatgaac atagacctac aaatacagtt acagtttttg ctgggcaaga taaagaagca
    41101 actgctacaa tgcgagaatt cattcagcag gtaccatcta gtccttcgta tgctttattc
    41161 aaaggtcgag atttagttta ttttatgcct agagaattta tcgaaggtag agatattaat
    41221 gacattgcaa tggacttaaa ggatgccttt gacgaaaatt gtaaatagta cacataaata
    41281 aatataaagg ttaacacatt ttataatatt aaaaatggtg tctgtcattg aaaatagaga
    41341 atatagttgt attccatttg ttatataaag tacgttctta ccaactatat tttctagaaa
    41401 tttaactgtt ttaataggac atcaaacata atattcaaat catgtgttaa cctctttttt
    41461 aaaatttttt agcattaaaa
//
LOCUS       DAPIDM010000009        40898 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000009 DAPIDM010000000
VERSION     DAPIDM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.009"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            397..1311
                     /locus_tag="RJE42_000399"
     CDS             397..1311
                     /locus_tag="RJE42_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDV5875853.1"
                     /translation="MYKINIIRSDSVYKNILKAPLNDRDSIFTKEILVPFKKKFEVQQ
                     IPIHNDAKQTMSAIQFLDAFQKSPKDLRMSDQMSIQYLNNDFWSNCKKYLKVAIDQFA
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSISTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPFLYGDDINKLQDRSIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            1290..2030
                     /locus_tag="RJE42_000400"
     CDS             1290..2030
                     /locus_tag="RJE42_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDV5875854.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPNKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQVSKLDKKISDLQLIRRSV
                     CEFIKGHSLIDTSILNKSLQTQYDKEASIKYGHTKAYQSFINRQDNLQSQDIRQKLTT
                     IFNQFNQMSLSHYPIQDCNDIVAEWKSFMNTIADFDDETLCCIAKTYENDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNENKKDNF"
     gene            complement(2283..2936)
                     /gene="nreC"
                     /locus_tag="RJE42_000401"
     CDS             complement(2283..2936)
                     /gene="nreC"
                     /locus_tag="RJE42_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDV5875855.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2959..3993)
                     /gene="nreB"
                     /locus_tag="RJE42_000402"
     CDS             complement(2959..3993)
                     /gene="nreB"
                     /locus_tag="RJE42_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDV5875856.1"
                     /translation="MINEDSIQLEALLKKYYEHSIEKIVFADDSGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTAKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGVKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(4017..4469)
                     /gene="nreA"
                     /locus_tag="RJE42_000403"
     CDS             complement(4017..4469)
                     /gene="nreA"
                     /locus_tag="RJE42_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDV5875857.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMIIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4489..5166)
                     /gene="narI"
                     /locus_tag="RJE42_000404"
     CDS             complement(4489..5166)
                     /gene="narI"
                     /locus_tag="RJE42_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV5875858.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAAGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNVQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKIHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(5159..5734)
                     /gene="narJ"
                     /locus_tag="RJE42_000405"
     CDS             complement(5159..5734)
                     /gene="narJ"
                     /locus_tag="RJE42_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV5875859.1"
                     /translation="MINFDNFKKYQESFGYIAQQLCFPEKLTFHPKTFEETISKDHPG
                     YDDLIAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5727..7286)
                     /gene="narH"
                     /locus_tag="RJE42_000406"
     CDS             complement(5727..7286)
                     /gene="narH"
                     /locus_tag="RJE42_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV5875860.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGNHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAADEKELYEKQLDIFLNPFDEEVIAQAEKDGISYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTNQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFDTSRLERLGLTERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7276..10965)
                     /locus_tag="RJE42_000407"
     CDS             complement(7276..10965)
                     /locus_tag="RJE42_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV5875861.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTDIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQSEWKPVIHDTISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNEDSVRTNRPRIDTARNVADAILNISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11272..12249)
                     /gene="cobA"
                     /locus_tag="RJE42_000408"
     CDS             complement(11272..12249)
                     /gene="cobA"
                     /locus_tag="RJE42_000408"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV5875862.1"
                     /translation="MSVNGYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYSKSTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGHFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLEKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPDDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADTNTVLCH"
     gene            complement(12240..12554)
                     /gene="nirD"
                     /locus_tag="RJE42_000409"
     CDS             complement(12240..12554)
                     /gene="nirD"
                     /locus_tag="RJE42_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV5875863.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12558..14963)
                     /locus_tag="RJE42_000410"
     CDS             complement(12558..14963)
                     /locus_tag="RJE42_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5875864.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHDIKLITNDPVVDVDRTNQNVTTANGIEVAY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVFAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWQDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGVISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(15009..15761)
                     /locus_tag="RJE42_000411"
     CDS             complement(15009..15761)
                     /locus_tag="RJE42_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDV5875865.1"
                     /translation="MNGNIIVAHGMRRGRQNQALEAFISELVKDDIHHYHIAFLESEH
                     QDLETVMTTLIQSGVKQLKVVPLLLFSAMHYLKDIPDIVHEMKRWYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGYEQQVGVVVVAHGNINGKFTKAHEELQTCVQHLQINKPT
                     YARTLYGEISFIHDLEDIARQYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLSDSNENIKALTKS"
     gene            complement(15989..16525)
                     /locus_tag="RJE42_000412"
     CDS             complement(15989..16525)
                     /locus_tag="RJE42_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5875866.1"
                     /translation="MANTTIRLANENDAETIHQLMYKAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEETFLRDTLKAAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(16931..17755)
                     /locus_tag="RJE42_000413"
     CDS             complement(16931..17755)
                     /locus_tag="RJE42_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV5875867.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSLNKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNFDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17958..18140)
                     /locus_tag="RJE42_000414"
     CDS             complement(17958..18140)
                     /locus_tag="RJE42_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875868.1"
                     /translation="MAQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSNLKKNK"
     gene            complement(18227..18694)
                     /locus_tag="RJE42_000415"
     CDS             complement(18227..18694)
                     /locus_tag="RJE42_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDV5875869.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSENNTAKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18880..20427)
                     /gene="adcA"
                     /locus_tag="RJE42_000416"
     CDS             complement(18880..20427)
                     /gene="adcA"
                     /locus_tag="RJE42_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDV5875870.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(20615..20713)
                     /locus_tag="RJE42_000417"
                     /pseudo
     CDS             complement(20615..20713)
                     /locus_tag="RJE42_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20720..21319)
                     /locus_tag="RJE42_000418"
     CDS             complement(20720..21319)
                     /locus_tag="RJE42_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDV5875871.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQKDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKGNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(21338..21700)
                     /locus_tag="RJE42_000419"
     CDS             complement(21338..21700)
                     /locus_tag="RJE42_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDV5875872.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQGFDS
                     RRYYQQHDADYHEENMTN"
     gene            21955..23205
                     /gene="fmhA"
                     /locus_tag="RJE42_000420"
     CDS             21955..23205
                     /gene="fmhA"
                     /locus_tag="RJE42_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDV5875873.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEVLEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHINRYNFYGITGDFSESAEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPMLYKLQTYLKHKRR"
     gene            complement(23302..24033)
                     /locus_tag="RJE42_000421"
     CDS             complement(23302..24033)
                     /locus_tag="RJE42_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV5875874.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVGKGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVFVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKAEAREKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAREVSNNIVFIHEGEIGEQ
                     GAPEEIFNNPKTEELKRFLNVINEE"
     gene            complement(24030..24749)
                     /locus_tag="RJE42_000422"
     CDS             complement(24030..24749)
                     /locus_tag="RJE42_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV5875875.1"
                     /translation="MFLNLNSEQLHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKVLRSIARVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYRQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(24730..25509)
                     /locus_tag="RJE42_000423"
     CDS             complement(24730..25509)
                     /locus_tag="RJE42_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDV5875876.1"
                     /translation="MKRLLFVVIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKAEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSKSAFAF
                     SKKVDDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(25630..27069)
                     /gene="mdeA"
                     /locus_tag="RJE42_000424"
     CDS             complement(25630..27069)
                     /gene="mdeA"
                     /locus_tag="RJE42_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDV5875877.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGIVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIEMMNQIKIDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSHQ"
     gene            27243..27347
                     /locus_tag="RJE42_000425"
     CDS             27243..27347
                     /locus_tag="RJE42_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDV5875878.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(27501..28187)
                     /locus_tag="RJE42_000426"
     CDS             complement(27501..28187)
                     /locus_tag="RJE42_000426"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV5875879.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(28514..29380)
                     /locus_tag="RJE42_000427"
     CDS             complement(28514..29380)
                     /locus_tag="RJE42_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDV5875880.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(29863..29982)
                     /locus_tag="RJE42_000428"
     CDS             complement(29863..29982)
                     /locus_tag="RJE42_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875881.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYEEMQYFANK"
     gene            30077..31387
                     /locus_tag="RJE42_000429"
     CDS             30077..31387
                     /locus_tag="RJE42_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B domain-containing protein"
                     /protein_id="HDV5875882.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     TKLQKNDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYTDKYFKYKGTIDKTVLSVFGNGYKSYIQPLKVEDQKNYVSKSYAQVRNYVT
                     ETLNTGKVLYAFYQNPKVVKAAITTAETASSIKNTLSNLLSFWK"
     gene            31923..32852
                     /gene="hlgA"
                     /locus_tag="RJE42_000430"
     CDS             31923..32852
                     /gene="hlgA"
                     /locus_tag="RJE42_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDV5875883.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFTEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHEKGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            33420..34367
                     /gene="hlgC"
                     /locus_tag="RJE42_000431"
     CDS             33420..34367
                     /gene="hlgC"
                     /locus_tag="RJE42_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDV5875884.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGNDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKNTNHIKS
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFKNRNYTVKYEVNWKTHEIKVKGQN"
     gene            34369..35346
                     /gene="hlgB"
                     /locus_tag="RJE42_000432"
     CDS             34369..35346
                     /gene="hlgB"
                     /locus_tag="RJE42_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDV5875885.1"
                     /translation="MNMNKLVKSSVATSMALLLLSNTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKAAGNINSGYERPNPKDYDFSKI
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(35398..35865)
                     /locus_tag="RJE42_000433"
     CDS             complement(35398..35865)
                     /locus_tag="RJE42_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDV5875886.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSVLLLIFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(35876..36568)
                     /locus_tag="RJE42_000434"
     CDS             complement(35876..36568)
                     /locus_tag="RJE42_000434"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDV5875887.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIDDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYISFL
                     ESTPKQVIYETV"
     gene            complement(36579..37694)
                     /locus_tag="RJE42_000435"
     CDS             complement(36579..37694)
                     /locus_tag="RJE42_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDV5875888.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDQVDFESFMRKYSYHLSSSRLISGSSTVYEEIESMLADWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAE
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGI
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHAEQDIDRLFEILHQED"
     gene            complement(37672..38679)
                     /gene="bioB"
                     /locus_tag="RJE42_000436"
     CDS             complement(37672..38679)
                     /gene="bioB"
                     /locus_tag="RJE42_000436"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV5875889.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYGTCENKENKNDVSRA
                     N"
     gene            complement(38681..40039)
                     /gene="bioA"
                     /locus_tag="RJE42_000437"
     CDS             complement(38681..40039)
                     /gene="bioA"
                     /locus_tag="RJE42_000437"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV5875890.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGLSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMAAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(40017..40703)
                     /gene="bioD"
                     /locus_tag="RJE42_000438"
     CDS             complement(40017..40703)
                     /gene="bioD"
                     /locus_tag="RJE42_000438"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDV5875891.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMADQIFLNKQCVLDKVKVLDK
                     EFDFILIEGAGGIGVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNHNVSARNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEEFLKQLI
                     GVKNELHTTT"
     gene            complement(40758..>40898)
                     /locus_tag="RJE42_000439"
     CDS             complement(40758..>40898)
                     /locus_tag="RJE42_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875892.1"
                     /translation="FYRIAVYICPNSVRSLSHFNSKVKILPKFIVILGSLNSCFLEKH
                     AP"
BASE COUNT        13400 a         7815 c         5793 g        13890 t
ORIGIN      
        1 tttttcaatt ctagtcaacc ttgccggcgg ggccccaaca aagagaaatt ggattcccaa
       61 tttctacaga caatgcaagt tggggcggga cgacgaaata aatttttcga aaatatcatt
      121 tctgtcccac tccccaatat agacaaagta ttgactacac atttatttta ttatgattgt
      181 gaaaaagtga acataaggat tttcccttat ttatggtgta atttccccta tacaatgtcc
      241 ctttacgatg catttagaca ctattttgtc atcattttaa tcagattatt aaggatcact
      301 cacatgcatt tcttaaattt ttttcaatta gttacaatta ctgcttgaaa atcacgttac
      361 gttacccttt atgctgacaa cgtaaggagg tttatcatgt ataaaatcaa tattattaga
      421 tcagattccg tctataagaa tatactcaag gcaccattaa acgaccgtga ttcaattttc
      481 actaaagaaa tattagtacc ttttaaaaag aaatttgagg ttcaacagat tcctattcat
      541 aatgatgcca agcagacaat gagtgcaatt caatttttag atgcctttca aaagtctccc
      601 aaagatttac gcatgtctga ccaaatgtca atacaatacc tgaacaatga tttctggagt
      661 aattgcaaaa aatatttaaa agttgctata gatcaatttg caaattatag tatctcatct
      721 caagtttcta actatcattt cacagtatta cttggagata gccaaaaacc acttatgtat
      781 ctaaataaaa atcgcggtgg tgatggtggc ataccaggtt atattatgat ttatttagtg
      841 ccaagtataa gtacaattaa ttctatgaaa agcttaattg cacatgaagt aaatcataat
      901 atgcgctatc aatatattga ttgggatggc ggaagtttga ttgaattgat tatcgcagaa
      961 ggattagctg aaaactatgt agaatctttg tatggtaaag cgcatattgg accgtgggta
     1021 acaaatacta attggagtcg tgataacgta aaaattaaaa atactattta taatcattta
     1081 catttaaagc atatatttga atcgatgcct tttctctatg gtgatgatat taataaactt
     1141 caagataggt ctatcgttgg cttatctcat gctgccgggt atgcatgtgg ctatcacttg
     1201 gtgaaatact ttttacaaaa aacaaacata cctattgaag ttgccacaac acttccagca
     1261 caaaaaataa taaatgaggt gactgaattt tggcatacac atacacttta aaagatatta
     1321 ttgaaattac aggtgtaact aaacgaactt tacattatta cgatgaaata ggattattag
     1381 ttccaaataa aaatgataaa aattatcgcg tttataaaca gcaagactta gaaaaattac
     1441 aaaaaatttt aatactcaag tcttttgatt ttgatatcgc taaaataaaa caatacattt
     1501 catatgataa tgaacaattg cgaaagttat tatcagagca agtaagcaag ttagataaaa
     1561 agatttctga cttacaatta attagacgct ctgtatgtga atttattaaa ggacactctc
     1621 taatagatac cagcatttta aacaagtcac tacagacaca atatgataaa gaagcatcta
     1681 taaagtatgg tcatacgaag gcatatcaat catttattaa ccgtcaagat aacttacaat
     1741 cacaggatat cagacagaaa ttgacaacga tcttcaatca attcaatcaa atgtctttaa
     1801 gtcattatcc aatccaagac tgtaatgata tcgtggcaga atggaaatct tttatgaaca
     1861 caatcgctga ttttgatgat gaaacattat gctgtattgc taaaacatat gaaaatgata
     1921 cgcgtttcaa agattacttt aattcatatg ataatcaaaa tttagcatca tatatttcag
     1981 aagctgttaa ttattttttg agcaatgaga ataagaaaga caatttttaa tcctcacata
     2041 gagactaaaa cactgaatac accctaacta acatgtcatg tccaaaatag acaactactc
     2101 atttattctc tatatcaata gctatattaa aagttattcc gtttccaata aaataagtat
     2161 ggctttccaa aaggagaaag tattagcttc catacggtta taaaaaatat atttcgaaac
     2221 atacaaaagc gccagaagat aatcatttcc tctggcgctt tgtatggtat taatttcaaa
     2281 ctctaaaact ctaataattt ctttttcaat gcatattcaa cgagctctgg tttacttttc
     2341 aagccaagct ttgtcataat atgcgtttta tgtgcctcta ctgtctttac agaaacaaat
     2401 aacttctctg caatttcttt attcccgtaa cctttggcaa taagtggcaa tatttctagt
     2461 tctcgttttg ataagatttt aaaaggatct gatgtgtttg cagtgtcctg atttgaatta
     2521 gatacaaatt cattcactaa agatgttgta agtttcatat ctacatatgt ttcaccttta
     2581 tataccgttc gaatagctaa caataattgt tcatcaggtg catttttcaa tatgtaacct
     2641 ttcgcaccat tacgcaacac atggaacaag tactcttcat catcaaacat ggttaatatt
     2701 aaaattttag tttcaggaaa actgtcagca attttactcg tagcgataag acctgactca
     2761 cctggtggca tgcttaaatc cattagtaac acatcaggtt tatattccat tactttttga
     2821 taagcttcga caccatctgc agccgttgca acaacttcca tatcattttg ataatttaaa
     2881 atcatagaga atcccgtgcg gacaacagcg tgatcatcgg caatgactat tttcaatttt
     2941 attcccccaa tgtatgtttc aaattggaat gttcagtgta acattggtgc cctcaccaat
     3001 tttcgtttcg atgttgacgc taccactgac taattcagct cgctcattca ttccatataa
     3061 accgagtcca gaacctttag gcttagaact tggatcaaaa ccatttcccg catctattac
     3121 ttcagcaacc aaatggcgcc cagtttgacg gatacctaca tttatttcat ttacatcagc
     3181 gtatttcaac gcatttaaaa tagcttcttg tactactcgg taaacaaccg tttcaatatc
     3241 actatcaaaa cgggtatttt taatattgga agtatatatg attttaaccc cataattttc
     3301 ttcaaattgt ttaaaatatg atttaaacgc tgcctcaagg cctaaatcat ccaaagaggc
     3361 tggtcttaat tccaccgaca tattacgtat atcatcgatt aatttagcga caatgtattc
     3421 aatgttttct gcgtcttcca aaagctttgc tgtatcttct tgatatttca acaatcgcaa
     3481 ttgaacatct acatttaaca tttcttgaat cacactatca tgtaattccc tagaaattcg
     3541 ttttcgctca ttttcttgtg cggaaatcgt tttacgcatc atacgttgtt gatgcaattt
     3601 ttcttgttgt tcaatttgtg atgagacatt ttgaagcgta aatgcatgaa tccctctatc
     3661 ctgatcaata agctgataag ttgcagtaaa cggcatcact ttttgatctt tcgtcttcat
     3721 aaacacttga aaattcgtag cttgtacttg catggactct aagaaacaat ctttacatga
     3781 ttgtacgtca taagcattcg tatacccttc acatctgtga caaatcgcat tcgctactgc
     3841 actataatta tcttcttcgg ataatatatc tttagctgca tcattcatcg caataatttt
     3901 accactatcg tcggcaaata ctattttttc gattgaatgt tcatagtatt tttttaataa
     3961 agcttctaat tgtatactgt cctcattaat catgacctac accctaattc atctcattat
     4021 ttatcatcat tgaaaatacc aaatttgcgt tgaatatcat cgttatcaaa tatttttggt
     4081 aaaggacgtc catctctttg accaaataat agtacaccat acacttgatt tttgtaccaa
     4141 agcggtacag ctaagactgc tgttaatgat tcgcttaata aaattggata gtcaatcttt
     4201 tcttctggtc ccaatgctaa accgacatta gctataatca tacgcttccc tgttttcata
     4261 acagttccag ctaatccacg gccttttctt aaaataatta atttaaaccg attattttta
     4321 ttacctgaaa catagtgcca ttttattgga gatgatggtt tgttagattc atagaaagcg
     4381 attgccgcaa aatcataacc ttcttctttg cgtattttat ctaatgtctc ttgaaatcta
     4441 cgatcttcaa taattgcttc tggtgtcaaa tcctttcacc tcttatactt acactttatt
     4501 cttacggtaa ataatatatc tgcgatttat atatgtcaaa ggtacactcc aaacatgtac
     4561 taaacgtgtg aatggccaac aagccataat agtgaaacct aataatatat gaattttaaa
     4621 tgcaattggt acaccactca ttaatgatgc atctggtttc aacataaata attgtctaaa
     4681 ccaaattgat aacgaagttc tataattaaa atctggatgc tgtacgtttg ttactaatgt
     4741 tgcataacat cccataaata caataagtaa taataagaag ttaacaaaga tatcggacgc
     4801 tgaacttaat ctgcgaatac ttttcgtagt aacgcgtctc gctgttaata aaaacatccc
     4861 taccaatgta attataccaa agatactccc tatataaaca gcaccaatat gatataaatg
     4921 ctcagacaca ccggcagcat ccatccacga cttaggtatt aataatccta caacatgtcc
     4981 aaaaaatacc ggaatgatac ctaagtgaaa tagcaaacta ccccacatca atctttttct
     5041 ttcaattaat tcactagatt ttgctgtcca cgaaaattta tcataacgat aacgtgcaat
     5101 atgacctgcg acaaagacaa ctaaacataa atacggaaat ataacccata aaaactgatt
     5161 aagcatgatg tttcactcct tttggtgatg tcaaacataa tttcaatgtt tttctaagtg
     5221 cttgaatcac ataggcatat ggattgttat cttcaccaag tgcattcgcc atcacatatg
     5281 ttccatcctc aataatcata atgattaatt gaatattctc ttcagctctt ggatcatttc
     5341 gccattctgc cacttgtaaa aattgcaaca tcaacggtag ataatcagaa agttcattgt
     5401 ctaccatttc tagtccaaac atctcgtaca atacctttag tttcgctaac atttgcccac
     5461 gttctttttg tgtatcaaac ttgttatacg tcatatataa tggcgctttt tttgtaaaat
     5521 caaatgtatc agtataaatc gctttgattt ctgataatga aaatgtcatc attacatttc
     5581 taaatgctat taaatcgtca taccctggat gatccttcga aattgtttct tcaaaagtct
     5641 ttggatgaaa tgttaatttt tccggaaagc atagctgttg cgctatatat ccaaaacttt
     5701 cttgatactt tttaaaatta tcgaaattaa tcacggaaaa tccctccata gaaattctca
     5761 ttataaattt cttgaccagt ttttcctgaa cctactgcaa caccacaacc ttcacagtta
     5821 tcaccaaagt gttcgccacc atagttatat cccgtactac cttgagcatg atacgtatct
     5881 aaatatgttt ccttatgtga cgttggaata acgaatcgat cctcatattt tgcaagtcct
     5941 aataagcgat acatatcttt agtttggcgt tcagttaaac ctaatcgctc taaacgtgat
     6001 gtatcaaatg gttgttgtgt aacttgggaa cgcatgtaac ttctcatcat tgccatacgc
     6061 tgcaatgctt cttttactgg ttttgtatct cctgccgtga aaatattcgc taaatattca
     6121 atcggcaagc gcatttcttc aatggctggg aaaatcgcat ccggattttg atttgtattt
     6181 ttaccttcga aatagctcat aattgggcta agtggtgggc aataccaaac catcggcatc
     6241 gttctaaatt caggatgtaa tggaaatgct agtttatatt caatcgctaa cttataaatt
     6301 ggtgagtttt gtgcagcttc aatccaatca taactaatgc catctttttc agcttgtgca
     6361 atgacttctt cgtcgaatgg atttaaaaat atatctaatt gcttttcata taattctttt
     6421 tcatctgctg cagatgccgc ttcatgcacg cgatccgcat cgtataataa aacacctaaa
     6481 taacgcatac gtcctgtaca agtttcagaa cacactgtag gcatccccgc ttcaattctc
     6541 gggaaacaga aagtacattt ttctgctttg ttcgttttcc agttgaagta tactttttta
     6601 tatggacaac ctgtcataca gtaacgccaa ccacgacaag catcttggtc aactaataca
     6661 atgccatctt catcacgttt atacattgca ccagaaggac atgaggcaac acaacttgga
     6721 ttcaagcaat gttcacacaa acgtggtaag tacatcataa atgtttcatc aaattggaat
     6781 ttaatatctt cttctatttt ttgaatgtta ggatcttttg gacctgtaac gtgaccacca
     6841 gccaagtcat cttcccagtt aggtccccat tcaatttcaa tattatcccc tgtaatttct
     6901 gaatacgctc tagcaactgg agaatgatta cctgatttcg cagttgttaa atgttcataa
     6961 ttatagttcc atggctcgta ataatcttta attagtggca tatctggatt atagaaaatt
     7021 ttccctaatg caatttttga aattctactt ccagatttta attcaagttt ccctttacga
     7081 tttagtaccc aaccaccttt gtaatgttct tggtcttccc aacgtttcgg ataccctacg
     7141 cccggcttag tctctacgtt gttgaaccac atgtactcag cacctggacg atttgtccaa
     7201 gtgtttttac atgtcacact acacgtatgg catcctatgc atttatctaa atttaatacc
     7261 atcgcaactt gcgctttaat cttcaagcca gttaacctcc ttcatctttc taactgctac
     7321 atataaatcc ctttggttcc caattggtcc ataataatta aagtgataac taatttgtgc
     7381 gtatcctccg actagttgtg ttggtttcaa atggattcta gtcggcgcgt tgtgtgaacc
     7441 accacgtgta tctgtaattt ctgacccagg cgtttgaata tgtttatctt gtgcatgata
     7501 cataaacatt gtacctttag gcatacgatg cgaaataact gctcttgccg ttacaacacc
     7561 attacggtta tacacttcta gccaatcatt atcttggata tcgtgttttt cagcatcttc
     7621 atttgatatc caaaccgttg gaccacctct aaatagtgtc aacatatgct tattatcttg
     7681 atacattgag tgtatattcc attttccatg aggcgttaaa taacgcagta ccaaagcatc
     7741 tgtaccacct ttaattttct tatctctatt accaaatacc attggcggca atgtcggttt
     7801 atatactggt aagctttcac caaactgttg gaaaacttca tgatcaacat aataactttg
     7861 acgacctgtt agcgttctaa aaggtactaa acgttctaca ttcgttgtaa atggtgagta
     7921 acgtcggcct tgtttattag atcccgggaa tactgctgtt ggtataactt cacgtggttg
     7981 tgatgttata ttcaagaacg aaattttttc agcagcacgc tcactagaaa tatcttttaa
     8041 cggcatacca gtttgttctt caagatcctc atatgatttc tgtgataact ttccattcgt
     8101 agctgatgaa atattaagta tggcatcagc gacattacgt gctgtatcaa tacgtggacg
     8161 attcgttctg acagaatctt catttgtgtc actccacgta cctaacatac tttttagttc
     8221 ttcgtactgc tcactgacac cgaaacttac accatgtgct ccaactttcc ctttttcaag
     8281 tacaggacca agcgtgacat atttgtcgta aattttagtg tagtcacgtt ctacgatagc
     8341 gaagttaggc attgtacgac caggtaccgc ttcaatttca cctttcgacc aatctttcac
     8401 tacgccatat ggtgtagata tttcttgttt tgtatcatga ctaagtggtg ttgtcacaac
     8461 atctttaaat gttccaggta aatagtcttt tgccatttct gaaaatgctt ttgccaatgt
     8521 tttataaata tcccagtctg aacgtgattc ccataatgga tcaatagcag gattgaaagg
     8581 atgtacatat ggatgcatat ccgttgatga taaatcatgt ttttcatacc aagtcgctgc
     8641 cggcaaaaca atgtcagaat ataacggtgt tgccgtcatt ctgaagtcta aagagaccac
     8701 taaatctaac ttacctgttg tttcctcacg ccacgtaatt tcttctggct tttcatcttc
     8761 atttggtgta gctaataacc ctgattttgt gccaagtaaa tgcttcataa agtattcttg
     8821 accttttgca gaacttgaga ttagatttga gcgccatata aacagtgatt tcggatgatt
     8881 ctttttcaaa tccggatctt ctatcgcaaa ttgcgtttgt tttgatttca cttcatcaat
     8941 tgcacgttgc aaaatcgctt catttgaatc tataccttca tctttagctt cttctgcaaa
     9001 caacaagcta tttttattaa attgtggata tgatggtaac caaccaagtc tagctgctaa
     9061 cacattataa tcagctggat gttgatgctt taactcctct gttttagcta atggagattt
     9121 taaacgatct acatttgact cttcatattt ccattgatct gtcgcaaaat agaaccaact
     9181 tgttccgttt tgcaaacgtg gtggcccttg ccagtctttc gcaaatgcga cagtactcca
     9241 tccttcaatc gggcgacatt tttcttgtcc cacatagtga gcccaaccgc caccattcac
     9301 accttgacag ccacataaca taactaagtt taagattgaa cgataaatcg tatctgagtt
     9361 aaaccaatgg ttaatacccg cacccatgat aatcattgaa cgcccttcag tatcgatagc
     9421 gttttgcgca aattctttag ctacttgaat gacaacactt tgttttacgc ctgaaatggc
     9481 ttcttgccaa gcaggtgtat attttgattc tgcatcgtcg tatccttttg attctaattt
     9541 atgatcaaaa cgacgcacgc catattgact tgccattaag tcaaaaattg tagcaatacg
     9601 gactttgtca ccatttgcta aagtgacttg tcgagttgga attgggcgat tgaatatccc
     9661 atctccatca ctatcaaagt atgggaattg aattgtttct aattcgtacc caccttctgt
     9721 cattgataat gtagggttaa ttttagaacc atcttctgtt tctagtttta agttccattt
     9781 cttaccttct tcccaacgtt gacccattgt gccattaggt accactaaac tgtcactgat
     9841 tgtgtcgtgg attaccggtt tccattcact ttgctctgtc gtttgtccta agtcacttgc
     9901 tcttaaaaat cgacccgctt tatatccatt ttcatcttca tctaacatga tgataaatgg
     9961 catgtctgta tattgtttcg cataattaat aaagcgttca ttcggttgat taacataatg
    10021 ctcttgtaaa ataacatgtg tcattgcttg agcaattgca gcatctgaac caggattcgg
    10081 tgctaaccag ttatcagcaa acttcacatt ttcagcataa tcaggtgcta ctgaaataac
    10141 ttttgttcct ttgtagcgaa cttctgtcat aaaatgtgcg tctggcgtac gcgttagtgg
    10201 tacattagac ccccacataa taatgtatga tgcgttatac cagtcacttg attcgggtac
    10261 gtctgtttgt tctccccaaa tttgtggaga ggccggtggt aaatctgcat accagtcata
    10321 aaaactaagc atttcaccac caagtaaatt gatgaatcga gcacctgctg catagctaat
    10381 catagacatc gctggaatcg gtgtgaatcc agcaattctg tcaggaccat attttttaat
    10441 tgtatatagt aattgtgctg cgataatatc cgtaacatct ttccaatttg aacgcacatg
    10501 tccacccata ccacgggctt gcttatactg tttggctttg tcttcatttt caacaataga
    10561 agcccatgca gccacacgat taccattgtt ttcttctaat gcttcagtcc ataaatccca
    10621 aagtttccca cgaatatatg gatatttaat tcgaagtggg ctgtattcat accaagagaa
    10681 tgatgcacca cgaggacagc ctctcggttc atattcaggc atatccggac cacaacttgg
    10741 atagtcagtt tgttgatttt cccaggtaat cacaccattt ttcacaaaga ctttccaaga
    10801 acatgatcca gtacagttaa caccatgtgt tgttcttact tccttatcat gactccaacg
    10861 ttctctgtac attttttccc attctctact tttactttct aggatcgacc aattcccatt
    10921 aaatttttct gttggcttaa agaaattcaa tccaaatttt cccatattta tatcctccta
    10981 cgtataaaaa tacgatgtgt agatgtcgtg tttttaaata ctaaaaatgc ccaagactat
    11041 tgctttaatt agattgtaca ttttttcaca aatataaaat attagggaat tacctaatta
    11101 cttaaggaat ttccctatca ataacgggat ttcattgaaa taatacacaa tcatgtgtgg
    11161 tcatgcttat tgccaatcta aatcgttcaa atttggcaca acgacaaata aggcttcaac
    11221 acgaatatat tctctcggtt gaaaccttac ttattcattt atttttataa attagtgaca
    11281 taacactgta ttagtatctg cacgatcggt tgaaatatat gttacatttt cttgttgctt
    11341 aataaacgca tcatagtaat catattgcga tgaatgatat gtgccattcg atgtatcatc
    11401 tgggtttagc aaacagccat aaccttcgtc atataaatgt tcacagagca taagggcgtc
    11461 atgtttagaa ccacttacta cataaaattg ctgagtcgga ttagaagttg ttgagacatt
    11521 ttctgtataa cccactactt cgcctataat acatatacct ggttttgcct caattgcgta
    11581 atgctccagt ttcccaacaa tattacttaa gcgcccttta acgacgaatt cattaaaaca
    11641 agatgcttga aaaacaatcg ctatcggata atcaatatct gtgtactgtt gtatctgggc
    11701 gataattttc tctaaacgtt taactcccat atatatcgcc aacgtaccac catttactaa
    11761 agcgttgaca tctacttcat tttcttctga atctttaaaa tgccccgtcg aaaatgtaac
    11821 acttttagca acagcacgca tcgttaatcc tgtttgcatc gtagcaactg cagcactcgc
    11881 agatgtcaca ccaggtacaa tttcaaacgc aatattaaag ttgtttagtg tgtcaacttc
    11941 ttcttgaact cgtccaaata ttgctgggtc gccaccttta agcctcacaa ccttgttata
    12001 tctacgtgct gcttccacga tacattcatt tattttttct tgctgaatgt gttttgcata
    12061 tggcttttta ccaacatcta taatctcagt tgtcgacttc gaatattgta aaatcaacgg
    12121 attcactaat cgatcatata gtataacatc cgcttcacgt attaaacgct cagccttttt
    12181 agttaaatag ttaggattac caggacccgc acctatcaag taaaccttgc cgtaaccatt
    12241 tacagacata tatatacgtt cccgtctgta acttctacct catatacatc tacacaacct
    12301 tcatcaggtt cttgtacaat cccagtattt aaatcaatct tttgatcatg gagtggacag
    12361 aatacatatt caccactcac tgttccctct gacaatggac cttgtttatg cggacaaata
    12421 ttgtgaatcg cgtgaattgt accactttca gttaaaaaca atcctatttc tttgcctttg
    12481 acaataacct ttttaccaat taagggtgtc aattcatcta aagttgtcac tttgattttc
    12541 tctttcgttt ccatgtatta caccttctcc acttcaaaga tttttcgagc ttgcgcatta
    12601 ctagtgattg cttcccaagg ttcagcttcg actgcttttt tagcatccat aatacgttca
    12661 aataattcat tctgtctttc tggatcaagc aacacttctt taacattttc aaaccctagt
    12721 cttcttaacc acggtgctgt tctttcagca taaatacctg tctcgcgata atattgcatc
    12781 aaagcaccac ataatttgat tacttcatct tctgtttcta cagttgttaa aaattcagct
    12841 ttttcaactt ctgtaccacc attaccaccg atatagattt ggaatccatt ttcaactgag
    12901 ataacaccaa aatctttaac acctgattca acacaacttc ttgggcagcc tgatacaccc
    12961 attttgaatt tatgaggtgt atcgatgtat tcaaatgttt tttctaaacg aatgccaagt
    13021 cgcgtcgtgt attgcgtacc aaatcgacaa aactctttac cgacacagct tttaactgag
    13081 cgtgttttct taccataagc tgatgctgaa cgcataccta ggtcttgcca tatatttggt
    13141 aattcttctt ttttaactcc atacaaacca acacgttgcg aacctgtcac tttaactagt
    13201 ggcacatgat attttttagc cacttctcct agacgaatca gttggtctgc atctgtaaca
    13261 cccccacgca tttgaggtat aacagaaaat gtaccatcat tttgaatatt cgcatggtaa
    13321 cgttcgttag caaatcttga ttctctttca tcttcatgat catgtggata aaccatattc
    13381 aaataatagt tgattgctgg tcgacatttt ggacatccac ctttgttttt aaagtttaaa
    13441 acatgtcgaa cttctttaga tgtttttaaa cctttcgctc gtatttgcgt aacaatttga
    13501 tcacgtgtca aatcagtaca accacatata cctgcaggtt tagcagcaac aaagtcatcg
    13561 cccaacgtgt gctgtaagat ttgagcaatt tgcggtttac atttaccaca tgaattacct
    13621 gctttcgttt tagccgtaac ttcttcaact gtagtaaaac cattttccgt aattgcattt
    13681 actatcgtgc ctttatcgac accattacag ccacaaattg tttcatcatc agccatatca
    13741 gcaattgata gcgatgcctc ttctccacct ttagtaagca atgataccag tgtgtagtct
    13801 tcagtagatt cacctttttt catcatgtta taaaagcgtg aaccatcatc gatatcacca
    13861 tatagtactg cgccaactac attaccgtct tttaaaaaga tttttttata gttattatca
    13921 acgctattaa atatttcaat acctttaatt tcagcatttt caacaatttg accagcacta
    13981 tataggtcac acccagaaac ttttaatgac gtaaatgttg ttgatccctt gtatccgttc
    14041 gtttctttat ttgttaaatg atcagctaat actttacctt gttcatataa aggtgcaact
    14101 aagccataaa ctttaccatt atgttctgca cattcaccaa ctgcaaatac attgctatca
    14161 cttgtttgca tcacatcatt gacaacaata ccacgattaa catctagacc tgattctttg
    14221 gctacttctg tgtatggtcg aatacctact gccataacaa ctaagtctgc cggaatctcg
    14281 cgtccatcag ccaatttaac accttcaaca tcatcttctc ctaagatttc agttgtgttg
    14341 gcttgcattt caaacttcat accttgcttt tctagatctg ctttaagcat atttccagct
    14401 ttacggtcta gctgcatttc catcaaccat tcagctaaat gtaacaccgt tacttccata
    14461 ccttgatcta ataaaccacg tgcacactct aaacctagta agcctccacc aattacaatt
    14521 gctttctttt tagtcttagc aatgttcatc atttgttcag tatcatcaat tgttctccag
    14581 ccaataacac ttggtaatgt cgaacctggc actggaatga caaatgcttt agatcctgtc
    14641 gcaaatatta atttatcgta tgctacttca ataccatttg cagtcgttac attttgattt
    14701 gttctatcca cgtcaactac tggatcattg gtaattaact taatatcgtg ttcctcatac
    14761 cactcatatg gattcataat tgtttcttca actgtcattt tattttgtaa aatatttgaa
    14821 agcatgatgc ggttatagtt tggataaggt tctttaccaa ttactgtaat atcatataaa
    14881 tcattggcac gctctaatat ttcttcgatt gttcgaatgc ccgccatacc gttaccaatc
    14941 attactagtt tttgctttgc cataaaatat gcccctttac tacataatat ttatttcaaa
    15001 aaaaggtgtt aacttttcgt taatgctttt atgttttcat tggagtcact aagactttct
    15061 aatctatcgt taataatttg ttttaaaatc gggtcgaaat taatagaagg tgtgatgtgt
    15121 atatctgtat taataaccat gtcattcatt tgctgcttca ctttgttaac aagtcttccg
    15181 tcatataaaa ataaaggtac gacaatcaat ttttgatact gtctcgcaat atcttctaaa
    15241 tcatgtataa aactaatctc accatatagc gttctcgcat aagtaggttt attaatctgc
    15301 aaatgttgaa cgcatgtctg taactcttca tgtgcttttg taaacttgcc attaatgttg
    15361 ccatgcgcaa caacgacaac accgacttgt tgttcgtaac ctgctaatgc gtcacaaata
    15421 cgttgttcaa ttaatcgtct cattaaagga tgtgtgccaa gtggctcgct tacttctacc
    15481 tttatgtctg gataccatcg tttcatttca tgaacgatat ccggtatatc cttgagataa
    15541 tgcatggcac taaagagaag caaaggtaca accttaagct gtttaacgcc actttgaatc
    15601 aacgttgtca ttactgtttc taaatcctga tgctcacttt ctaaaaatgc aatatggtaa
    15661 tgatgtatgt catcttttac taattcagat ataaacgctt ctaacgcttg attctgccga
    15721 ccgcgcctca tgccatgtgc aacaatgata ttcccattca catttaccaa ccctttcaca
    15781 cgtattgtat accaaatcat ttcgtttttg tgaaaagaat cacattataa tgtaaaatca
    15841 gggaattccc tgatgcctgt agtcatgcat attccttata aattttccct ttttgttaaa
    15901 tcaaaaaagc gaccatatat gaatccctac tcaacattta tttgagcaag catcaatata
    15961 tcggtcgctt gtagtgtata ttattatctt aaaatggtgg ttggcctaat attgtttcgt
    16021 caaagcgctc gggtatcaat actttgcgca tgatcacacc taaatcgcca tcatcatttt
    16081 catgttcgct gtatatttca taacctcttt tttcataaat tttaagtaac cacggatgca
    16141 atcttgcaga tgtacctaaa gttacagcag ctgcctttaa tgtatctcgt aaaaatgttt
    16201 cttcaacata agtaagtaat tgactaccat agcctttccc ttcatactca ggatttgtcg
    16261 caaaccacca gacaaaagga tagcccgaaa tgcttttcac acttccccaa ggatatctaa
    16321 ccgtaatcgt agatataatt tcatcatcaa ttgtcatgac aaatgtagta tttttatcta
    16381 tattttcttt cacagcatct aaatttgcat ttaccgaagg ccagtcaatt ccaagttctc
    16441 ttaaaggtgt gaatgcctta tacattaact gatgtatcgt ttctgcatcg ttttcattcg
    16501 ccagtcgaat cgtcgtattt gccatttgaa tccacacact ttctgtttat tttaaatgaa
    16561 tcatctttaa ttttagctat gcttaccttt tatagcgtac caacatttag cacatgtgtc
    16621 agataacatg cctcattgat ataagataag tatatgatca tcaattattt cattattgaa
    16681 agcaaagtaa aagtctagga catgaattaa tgatatgtac gcttcaaagc tttcattttt
    16741 caatgtctac tttgaagcgt gcagttcata atgtcctaga ctctacaatg ttatattggc
    16801 agtagctgac tgcggagccc aacatagaga atttcaaaaa gaaattctac agacaatgca
    16861 agttggcggg gcccccaaaa aggatgactc taaattcatt gttgtcacaa catcttcaat
    16921 tatgactcca ttactgttgc tctaaacgat tgcgtttacc atttaaaaat gcataaacaa
    16981 gtcctacaaa aataccgcca cctacaaagt taccaaggaa tgcaaaaacg atatttttta
    17041 atacatgtaa ccaagataca gcatcaaagt tgaagaacac catacctgca tataaaccag
    17101 cattgaatac aacgtgctca tatcccatgt atacaaagac tataacacca caagcaatga
    17161 agaatgcttt tgtcaatccg cctttgaatt gcatagaaat aaaaataccg atattaataa
    17221 agaaattaca gaaaatacct tttgtaaaaa tattcaacca agtcgaatcg actgtctttt
    17281 tctgaactaa ggcagttaag gcttgtgtca tttctggtgt cataacatgt gcatatttca
    17341 ttaagaagaa taatacaaaa ccacctaaaa tatttccaag gaaacaatat aataaaatcc
    17401 acatcatctt attaagagaa acaactttat aataccagcc aactgtaaaa tacataaaat
    17461 tacttgttaa taattcagaa ttcgttagta caaccaagac caatcctaag ctgaaagcaa
    17521 ttgctcccat taaattaatg agaccatcaa catgtgtact tgcgaattgt gttttaatac
    17581 caaacataaa aacagtaaca atggacaata agaaacctgc catcatagat ttaagcatgt
    17641 atcgtcctgg tgtttgctgt accatcactt gtttcatttg aacagatgat gatattgagt
    17701 ctacgattgc tttagttgaa tacgtatctt caactgtttt attatttttt atcacagcac
    17761 ttcccccatt tactattatc aaataaattt tacctattaa ttatattagt atttagatgt
    17821 aattcataga ttaaaattca gaaaattctt tttaccaatt caaaactaat atttactcat
    17881 aaaaatagcg agatagatat cgcgacataa aacaattggt atcattttaa ttgcgctcat
    17941 ctcgctttat gtaagtatta cttattcttt tttaaatttg aaaagtaatg taaaccatat
    18001 gcaaatatac cgtagcctac gactgtacgc aaaataaatc taacaacttt atttggtaca
    18061 catttcgaag taacagtaat tgaagataaa agcgttgtaa aaatatataa tattaatact
    18121 ttaatgtaac gttgtgccat cgaatcaccc ttcattcaat taataatatt acgttattat
    18181 tataaactga atggcatata aaatttttta catattttca acaactttat ttcaataaag
    18241 catcaagttg atctaatgtt tgattcatcc cttgctcaac acccatgttt atgacttgtt
    18301 gagcggcttc ttttgttgga aataccgatg ttgatgtcac tgtcgttttc gctgtattgt
    18361 tctcggaaaa tgacaaagta atcttcatgc ttggcatttt cgtatctttt gcaccttgag
    18421 gtgtcgcaaa tgagtcaata tattcaatta aatatggacg cttaaccgtt ttatattctg
    18481 ctaatgtata gctggtcatt gtaggtgttt taatcgcgta aaatgcatca ccgcctgaaa
    18541 ccgcattaaa gcgaaacact ttggtactag cgtcttttgg atgaaaccat ttttcaaata
    18601 atgcttttgt cgtataggca tcaaacacct tttcaatcgg tgcttctatc gttcttgaaa
    18661 aaataatttt attatcttca actttaatcg tcatcttccc actccctatc ttttatattt
    18721 actcatctta acatgcattg agtgacatat gttaccaaat catttgttat aaactatagt
    18781 aatattaatc attttctctg ttgaaattga taactacgaa ataaaaaaca cactctattc
    18841 agctattaac agttgagtag agtgtgcaaa cctgttgaat taatgcgcta acatttcttc
    18901 tttgatttgg tctttattta attttgaagg ataatatgtt ggccagttat ccatttcttt
    18961 taataacgcg tcattatcat tacccataaa gatatggaag tgttctgcct ttttaggtgc
    19021 aatgttgtga tcgctaaatt ggatgaattt cggtaagtct ttatcattac catcgaccaa
    19081 tttaaacata aatctcacgc cacggttacc tttaggatat ttcaatgttt tcttaccatt
    19141 gtattcatat ttaccagtgt gtttcttacc atctttagta aatgtaattt catttccttt
    19201 tatatcaatg ttagtaatat cagttttata tcctttgtca taataagctt ttaaatcttt
    19261 agcagatttc ttcggatcat tttcagcttt atgttccatc acttcatcaa gcgtaccgtc
    19321 ttttaagtaa gggtaaacag attgccattc accagcataa tcgcttaatt cacggtcttt
    19381 aacttgctca tctttaaaat acccatcaga aattgctttg tcgtgtttac tttcagcttt
    19441 gtcgtctttc actttaacac cactgtctaa agctttaccg atattttcaa tattcgattt
    19501 cattaatgat tgatacgtaa tattatcttt tttctgttgt tctttattta aagactccat
    19561 gttgtagaat tttaaaggct tcgcatctgt ttctttacga attgtttcag tcactttatt
    19621 cgcaacatta tcttcataaa gaatatattt tgcattgcta tctctaattt ctttaacaat
    19681 tttagttaat tctttttgtg atggatcttc agcattcatg ttttgaatac ctttttgaac
    19741 aaaaccataa cgatcagcta agtatccaat tgattcatgt gaaatgaata ctgcattacc
    19801 ttgcttatct tttgtaactt gcttcatatc gttatcaatt ttcttaagat cgtcgtttaa
    19861 ttttttgtag tttttctcat agtcatcttt atgttttgga tctttcttca ctaattcatc
    19921 tttaatttct ttagcaaaag tttggttaat tttaggatct aaccatacgt gtggatcata
    19981 tccgccatga tgatgatgtt cttctttctc atgatcatgt ccctcatgtt catgctcttc
    20041 accatgttcg tgttgatcag ttaaaagctt tgctttatct aatttatctt ctaaagacag
    20101 ttttttatct ttatctttga tagtagatgc aactttctta gcaaccggat ctaaattatc
    20161 ccctgtatac ataaacaagt ctgacttgct tgcacttaat atatcttttt gtgttggttc
    20221 atagctatgt aaatctgtcc ctgctggata gattgatgat accttcacgt gttttccacc
    20281 aatttgctct gcaaatgatt gcaatggata aactgtcgtt ttaattgtta ccttgccatc
    20341 tttatctttt ccatcatcat tcccacatgc ggctaatgat aaagttacgg ctggtacaag
    20401 aagtaacata cctaatttct ttttcatagt caccctccta aatagtaatc tttacgtttt
    20461 aaaattatat actcatgcta cttaaaatac aagcactgtt tttataaata atataaagtc
    20521 gaatcatact ttaggtattt accacttttc gttttatcta atacatgact attgtacaaa
    20581 tatgatacaa aaaactttat agtatttacg aacatcataa tataagaaaa aagacaatct
    20641 ctatcatatt gctaatagaa attgtctttt catattgtcc taataaattg gtatcactta
    20701 cttatctcaa caattaagac tatttgattt tatcttttaa taacttttca taactttcat
    20761 aatcatatgg atcttcaact ttctcgccat taataaatgc agttggtgtc gtttttatat
    20821 gattaccttt agctattttt ttatctttct ctgcagcttt ccaagacttg ctatcttttg
    20881 tcttgtaatc ttttataatt ttattttccg tctctttatc taaatgcagt tgtttaatat
    20941 gtttatctaa tagttctttt gttaaccatt ctttattttc atctttctgc gcagcaaata
    21001 attgcttttg aaaatctaaa aatgatttag gtgcatacat caatactgca tgactcgcac
    21061 gcgaaccaac aattgagtct ttacctaaga aagctaaatt gacaaattgg tattccactt
    21121 tgtgattatc tatataattt ttacgcaact ttggcatgac tttttcatct aactctttac
    21181 aataaggaca tttatagtcg ccatatatga caactaatgg tttgccgttt ttcgaagatg
    21241 tagttgctga ttctttttta ccgcaagctg ttaaaattaa catgctcact ataaataatg
    21301 ttagtaattt tttagtcata ataactccta ttcatatcta gttcgtcata ttttcttcat
    21361 gataatccgc atcatgttgc tgataatatc gtctcgaatc aaaaccttgc ttatatgata
    21421 cacgtttatc actaaaatca tatgcataat aatgcacttc atcatcatta ctcaaaggtt
    21481 tataagctaa aatgataatt ttatccttat catagacata aatattagca tctttgcgtt
    21541 caaaatgatc aacacgatca ccgttgcctt ttttggcaag ttgttcttgt ttttgatttt
    21601 gaattttaac tgcttcatct atttctttca catatttatc attaccacat cctgatataa
    21661 agactagtaa taataatacc gtcgccacaa atcgcttcaa tgctcccact cctactatca
    21721 acttccaact aactttcaat ataattaata ctatacaccg tcaaacatca atgcgctaca
    21781 actactacca atgcatttaa tataaatatc caaagaaata acagttatgt atagtcaaag
    21841 aaaaatatat ttcattttct atgtgtattt gcaatcaatt tttgataaaa taaaaaaata
    21901 gcattatata taaattcaaa aattacagtt caattcagag agggatggga taaaatgaac
    21961 tttgtaacgt tgacttcgga tgagttcaat gcgtttacaa caaagcattt ttcacattac
    22021 acacaatcag ctattcatta caatcataga gttgatttaa aaggcgatgt gcatcttgta
    22081 ggggttaaag atgacaatgg tcaagtgatt gcaggatgct tattgacaga agcacgcaca
    22141 cttaaatttt tcaaatattt ttatactcat cgcgggccag tgatggatta tacaaatcaa
    22201 tcattagtag catttttctt taaagcatta acgtcctatt taaagaaaca aaattgttta
    22261 tatgtccttg tagatccata tttaattgaa aatttacgca atgcagacgg tgaaattgtt
    22321 aagtcttatg acaaccgagc atttgttaga acaatggaca cattaggcta taagcatcaa
    22381 ggctttccgg tcggttatga ttcaatgagt caaatccgtt ggttatctgt attagattta
    22441 aaagataaaa cagaagacca gttattaaaa gaaatggatt atcaaacgag acgtaatatt
    22501 aaaaaaacat atgatattgg tgtcaaaact aaaacattaa cgattgatga aacgcaaacc
    22561 tttttcgact tattccatat ggccgaagaa aagcatggtt tcaaattccg tgagttacca
    22621 tactttgaag aaatgcaaaa gttatacgat gaccacgcca tgttaaagtt agcttatatc
    22681 gatttaaacg agtatttaaa aacgttacaa ttaaagcaac agcaattaac agctgaactc
    22741 tcaggtgttg aagaggtatt ggaagaaagt cctaattcaa agaaaaataa aacgaaacgc
    22801 acacagctag aacaacagct gaatagcaat aagcgtaaaa tcgataatac aatagaacaa
    22861 attgaacaag acggtgcagt gttaaactta gcttctgctt tgtttatcta caatgagcat
    22921 gaagtttatt acttatctag tggttcaaat cctaaatata atgcttatat gggtgcctac
    22981 catttgcagt gggaaatgat taagtttgct aaagcgcatc atattaatcg ctataatttt
    23041 tatggcatta ctggtgattt ttcagagtca gcagaagact atggcgtaca acaattcaaa
    23101 aaaggattta acgcacacgt agaagaatat attggtgatt ttattaaacc aatcaaacct
    23161 atgctttaca aattacaaac atacttaaaa cataaacgcc gttaacagtt tatgctgtaa
    23221 catctagaat ataacaaaga accacgcatc atgtatatgg catcatcgcc atattaaaac
    23281 atgatacgtg gttctatttg tttattcttc attaataaca tttaaaaaac gttttagttc
    23341 ttcagttttt ggattgttaa aaatctcttc aggtgcccct tgttctccta tttcaccttc
    23401 atgaatgaat acaatgttat tagatacttc tctagcaaaa cgcatttcat gtgtcacaat
    23461 gaccattgtc atgccctcat tcgctaaatc tttaatcacc tttaacacat cattcacgag
    23521 ttcaggatca agcgctgatg ttggttcatc aaacaacatc actttaggat tcatagctaa
    23581 tgctctcgca atggcaacac gttgctgttg accacctgat aatgcatgag gtcgttgatc
    23641 tttgacatgt gtcaaaccaa ctttctcaag taattctaga gatttctcac gagcttcagc
    23701 ctttttcaac tttttaactg taataagacc ttccattaca ttttctaatg ccgtcttatg
    23761 cggaaaaaga ttatagcttt gaaataccat accagattgt ttacgaactt ctatttgtga
    23821 ttttttatct ttagatgtat atgttttgcc gttaacaaat actgtacctt cagttggaat
    23881 ttctaacgca ttaatcattc gtaacaacgt tgttttacct gacccagatc ggccaattaa
    23941 ggttacaacc tcaccttttc caacagataa atcaatacct ttgatgactt caacatcatt
    24001 aaatgattta tggatattgt tcaattgaat catgagcgat accctctttc aatataagat
    24061 tcatagatac cttggattat tgaaataata aagcatacca cccagtacat tagcgcaact
    24121 aacacataaa ttgttaaata ttcatatgtt gtagacgcaa cttcttgagc ttttctaaac
    24181 atttctgcta ctaaaataaa tcctagtaat gatgtatctt tgattaaact taaaaatgta
    24241 ttacctagtg caggtataga tactcgaatt gcctgcggta aaatgatgcg ttgtatcgtt
    24301 tgacgatatg tcataccaat agaatatgca gcttccgttt gtccttttgg tatagaaata
    24361 ataccgccgc gaataatttc tgaagcatac gcacctacat ttaatgacaa gccaataatg
    24421 gcagcgacca caggtgccaa ggtccattga ttatctgcat cattggttaa taatctacct
    24481 aattctggaa taccataaaa gatgataaat aattgtacta tcataggtgt cccacgaata
    24541 atagatacat agacacgtgc aatacttctt aaaactttac tcgttgaaat gcgcatcaat
    24601 gctgtaaata atgcaatgat taaccctaat acaaatgtta cgagggtaat cggaattgaa
    24661 tatttcacca aaccttcgag cataggccca aaagcttgtt ttgcagcatc aagtgcatgt
    24721 agttgttcac tatttagatt tagaaacatc ttgaccaaac catttcttac ctattttagc
    24781 taattcaccg ttttcctcga tttttttcaa gccatcattg aatttttgaa ctgtttcgtc
    24841 atccactttt ttagaaaatg caaatgcaga tttactttgt tcagcgttac ctttgattgc
    24901 cttgatctta gcattaggtt tttgtttttt ataatccaag tatgacagac tgtcattaaa
    24961 tgtaccatca acacgcttag acaataataa atccattgat tggttaaagc catcaacttt
    25021 tgtaatatca gcacctttat ccttagctaa tttaccataa ttagatgtga atgtttgtgc
    25081 taacttctta cctttaacat catcaaaatc tttaatatct ttttcatttt cacgaataac
    25141 taaaacagca cttgagaatg tgtaaggctt agaaaattta tatttctttt ctctatcagg
    25201 attaataccc acttggtttg cgattacatc aaaacgccct gcgtctaaac ctgcaaacat
    25261 agagtcccaa gaagtttcgt taaatttaag ttttaatcct tctgctttag ccactgcttt
    25321 aataacatcg atatcataac cagttaattt accatcttta tcatggaaac taaatggtgc
    25381 ataagtcccc tcagtaccaa cattaattgt cttactatct ttactagctt ccttgtcttt
    25441 agacgaattg ttcccacatg cagctaatat gaaaacgaaa gctatcacca caaataaaag
    25501 tcttttcata gaatatatct ccttattctt attattctaa tcggaataaa aatattttaa
    25561 tctgaatcgc cagataagtc aatatgttga acttttgcta aaaagcgtta tatagctagc
    25621 aacttatatt tattgatgtg aatcaacttc ttttttcttt ccttgtaaca taaatgaaca
    25681 aaagaaacta atcactgtaa tcaatagcgc aaataaaaat ccagcgtgat aaccatgtaa
    25741 catcgtatca attttaattt gattcatcat ctcaattttg ttcatgccat tatagtctga
    25801 cattgttggc gaaaagtttt tacttgcatg agataaaata gtaattaatg cagctgttcc
    25861 gatagaacca gcaatttgtt gtactgtgtt tgtcatagat gaaccgtgtg cattcatttc
    25921 tggctttaat tgattcatcg tatgggtcat tactggaatt aatcctaacg cgataccaac
    25981 cattcgaatc gcataaactg ttgccaacat gattgttgat gtttgttcat ccattacaac
    26041 aaaataagcc gtagtaataa caacaatcgc catacctact aaagcaagct ttctaggacc
    26101 aaacttttca tataaagcac ccgaagtcat cgacataaat gccataacaa ttgctcctgg
    26161 taataaagtt aacccagaat ctaaagctga acgatgcaat aaattttgca caaagatagg
    26221 taaaatcgtt tccgaaccaa tcatcgatac cattgtgaca cccataataa tgataccaac
    26281 tgtaaattgt ttatttttaa atactgcaaa atttaataat ggtgttgata gccgatattg
    26341 acgtaataca aataatgcga ctaaaacaat accaccaatc atagatacta atacgatagg
    26401 attatcaaaa cctttttctg aaattgaact cgttccatac aataacccgc cgaaacctag
    26461 tacagaaaaa atgacagaaa taaaatcaat aggtacttta attgtagttc caacattttt
    26521 tatcgtttta aatccaatta aaaatgtcac agctgcaatt ggagcgacaa catggaaaag
    26581 cgatctccag ttaaaatatt caacaaggta acctgaaagt gtaggtccaa tagctggtgc
    26641 taaaccaatt accaaaccaa atatccccat atatttaccg cgttcatgcg gttggaaaac
    26701 atccaatatc gttgtcatca tcaatggcat cataatacct gcacctaacg cctgaataat
    26761 tctagcaact aataatgttg caaagtgcgg actcaaaccg ccgagtaatg tacctaaaaa
    26821 aaagatagaa atacccacta aaaacacttg tcttgttgta taacgttgta taacaagcgc
    26881 ggacaaagga ataacaatcc cattagtcaa taaaaaagct gttgtcagcc attgaacttg
    26941 tgtatatgaa atttcaaaat ctttcataat acttggtaaa gccgtcacta atagtgtttc
    27001 atttaataat ccaaaaaatc cacccatcag catagtaata atcatcgtta atttttgttt
    27061 cttcgacatt taaagcttcc ccctcaccat ctaaaaaacg acacacattt ttagatatcc
    27121 ggtaattata cccattttca aaaaaaatag cacgacttta ttacaatttc tttaaaacgt
    27181 aatcattact atttacatat gagtagttat cggtcataat atatttacaa ggagggatag
    27241 agatgaaatt agatttacag actgctcgtc gtaatttaaa tagccctaac attaaaacaa
    27301 gaaagcgtgc tttaaaaatt attaagcaac acaaaagagc gaaataattg ttttaatttg
    27361 actatattgt atgtctaact atattttcga gtatccctag ctcgttaata taaactctat
    27421 acttaactat ttccttacat tacttcacat atttaagagc tgcatataca ctcactcctt
    27481 gtgtacatgc agctcttttt ttataagtag tatttatcta taacttctaa atcatccgtt
    27541 aattcataaa caagcggtgc acctgtttta atttcataat taatgatatc ttcatctgac
    27601 acatcttcaa gatatttaat caatgcgcga attgaatttc cgtgtgcaga aactaatacc
    27661 gtttgaccat ctagcaaata ttgtgaaata tgatctgtcc aaaatggtat cactcgaact
    27721 aaagtatctt tcagactttc ggaataaggc atcatacgtt tatctaaatg attatatcga
    27781 cgatcagcta agtaagcttc acgttgttct tcggtttcag caggtggttt cacatcataa
    27841 gaacgacgcc aaatatgtac ttgttcctct ccaaattctt ttctagcatc atctttattt
    27901 aagccttgca atccaccata gtgacgctca tttaaacgcc agcttttata tacaggaatc
    27961 cattgttgtt tagattcagt taaaatataa tgcgttgtat ctaaagcacg tgttaataac
    28021 gatgtaaaag ctacatcgat ggcaatgtta ttttctctta ctttttcacc tgctctagtc
    28081 gcttcattaa taccttgttc agataaatta acatcttccc atccagtaaa taagttttta
    28141 gcattccact cgctttgtcc atgacgacat aaaattaatt ttggcataac ttataacctc
    28201 tttcctttaa tctttaggac gttatttcag ggatatttta agatgattta atcttagcaa
    28261 ttttcttttc aatatgtttt aaaattcaat gattaaatca attatcacta gacattaaat
    28321 acataaatcc tattccccat tttcattttt taattcataa atgaatcaat aaccacctaa
    28381 taacaaatca tattatacac ctttgtccgt catttttcta aggtttaaaa aatattttta
    28441 ggtgcaccta aaaataaatg taataaaaac gcctcctcag atatttatat atctatgaag
    28501 acgtttaaat acattataga tggtctggtt ctgggtgaac gtatactgag gaaatacctt
    28561 ttttgtgcaa atgatgttcg acattatcac aaatttgatg cgcttctact aaggaaaggt
    28621 tagcatctac aacaattgtg acatcaataa acacactact tccatggtaa cgccctttaa
    28681 tacttttaac ttcttgtact tcatcaactt ctaaaatatc attgcgatac gcttctaatt
    28741 cagtttcatt gaaaccatca ctcaacataa aaattgcttc tttaaaaata ccaaaaccag
    28801 tataaacgat tagtaagcct agcaatgttg ctaaaataat atcgacaatt gggaaaccga
    28861 tttgcgtaaa aattaatcct atcgccgttc caatgctgac taaactatct gataaattat
    28921 ctttggcagc agaatttaaa gaactacttt tcgttctttt cgctagtctt tgattgactg
    28981 caaatacaat caacattaca aggccactga ttaagctgac gataattgtt attgcgttag
    29041 gtacaacgtc atcttctttg aacaaacgag gtgcattttg aataactact tggataccta
    29101 caaacataat gacaaatgac accaataatg aagaaatatt ttcagacttc aaatggccat
    29161 aaggatgatt tcgatcggca ggtttaattg aaattttcaa tccaataata acagctaaag
    29221 aaacgataat atctgtcata ttgtttaatg cgtcggctct tacagctgca gagttaaaga
    29281 caaaacccgt gacatactta acaatagata agattatata tacaattaaa ctcaaataag
    29341 caccgcgttg cgccaattta agattttcat tatgagacat gcgttgaacc accttgaatt
    29401 agtatagtaa caatattatg aatgatttgt tttaaattta caacgttttt aatttttata
    29461 aatttttata aaaattaaac taatttattc attcgcaacc cctaaaaata ttttttagcc
    29521 tttcttcaaa tttatttgtt agaaaggctt atgcagttga cgttttacgt ccaactgcgt
    29581 tccttcgtct tcttcaaatt tatttgttag aaaggcttat gcagttgacg ttttacgtcc
    29641 aactgcgttc cttcgtcttc ttcaaattta tttgttagaa aggcgcccaa ctctccctgc
    29701 ttgttaactt tcgcctcgaa agtttctatg ttgggaccct atgcattagt tgcgaagcta
    29761 tctaatgtag tggtctaatt atatagaaga aaaagtgcat cagtgacaaa ttaaatgaga
    29821 tttctactct accaaactct cttcgaaaga cagttttctc ctctatttat tagcaaaata
    29881 ttgcatttct tcatatagta cttccttact taaaatacgc tgaatgttct gaattaaacg
    29941 cttttttaca tagttaacac tagttaatct attagttaac attagttaat aattagttaa
    30001 tttccatttg tattctcatg tgataaattc taaaagcata caataaattt aatatgtaaa
    30061 aagaaaggga atacacatga aaaataaata tatctcgaag ttgctagttg gggcagcaac
    30121 aattacttta gctacaatga tttcaaatgg ggaagcaaaa gcgagtgaaa acacgcaaca
    30181 aacttcaact aagcaccaaa caactcaaaa caactacgta acagatcaac aaaaagcttt
    30241 ttatcaagta ttacacctaa aaggtatcac agaagaacaa cgtaaccaat acatcaaaac
    30301 attacgcgaa cacccagaac gtgcacaaga agtattctct gaatcactta aagacagcaa
    30361 gaacccagac cgacgtgttg cacaacaaaa cgctttttac aatgttctta aaaatgataa
    30421 cttaactgaa caagaaaaaa ataattacat tgcacaaatt aaagaaaacc ctgatagaag
    30481 ccaacaagtt tgggtagaat ccgtacaatc ttctaaagct aaagaacgtc aaaatattga
    30541 aaatgcggat aaagcaatta aagatttcca agataacaaa gcaccacacg ataaatcagc
    30601 agcatatgaa gctaactcaa aattacctaa agatttacgt gataaaaata accgctttgt
    30661 agaaaaagtt tcaattgaaa aagcaatcgt tcgtcatgat gagcgtgtga aatcagcaaa
    30721 tgatgccatt actaaattac aaaaaaatga ttcaattgaa aacagacgtt tagcacaacg
    30781 tgaagttaac aaagcaccta tggatgtaaa agaacactta cagaaacaat tagatgcgtt
    30841 agtggctcaa aaagatgctg aaaagaaagt ggcgccaaaa gttgaggctc ctcaaattca
    30901 atcaccacaa attgaaaaac ctaaagcaga atcaccaaaa gttgaagtcc ctcaaattca
    30961 atcaccaaaa gttgaggttc ctcaatctaa attattaggt tactaccaat cattaaaaga
    31021 ttcatttaac tatggttaca agtatttaac agatacttat aaaagctata aagaaaaata
    31081 tgatacagca aagtactaca ctgataaata ttttaaatat aaaggtacaa tagataaaac
    31141 tgtactatca gtttttggta atggatacaa atcatacatc caaccattaa aagttgaaga
    31201 tcaaaaaaat tatgtatcta aatcatatgc acaagtaaga aactatgtaa ctgaaacttt
    31261 aaacacaggt aaggtattat atgcatttta tcaaaatcca aaagtagtta aagctgctat
    31321 aactacagct gaaactgcat catcaatcaa aaatacatta agtaatttat tatcattctg
    31381 gaaataatca atcaaaaata tcttctctag ttttacatca ttttttaaat aattttcgta
    31441 ataaaccgtg attaaaaaga accgttgatt ctcaatcgaa tctgcggttc ttttttcatt
    31501 ttccatcaat taaatgtttc ttcgctattt gtcagctcac ttttttacct gcaacttgtt
    31561 aaataatcct tacatcgtta acgaatagtt catcatttag ttgaatcagc tcaactttat
    31621 taacttcaaa atttcacaaa ctattgcaca atccattcct ctaccacttc aagcacctta
    31681 attaaacatt aattaaatag aataagactt gcaaaacata gttatgtagc tatataaacg
    31741 cctgcgacca ataaatcttt taaacataac ataatgcaaa aacatcattt aacaatgcta
    31801 aaaatgtctc ttcaatacat gttgatagta attaactttt aacgaatagt taattcgaaa
    31861 acgcttacaa atagattatt atatatatga acttaaaatt aaatagaaag aaagtgattt
    31921 ctatgattaa aaataaaata ttaacagcaa ctttagcagt tggtttaatc gcccctttag
    31981 ccaatccatt tacagaaatt tctaaagcag aaaataagat agaagatatc ggccaaggtg
    32041 cagaaatcat caaaagaaca caagacatta ctagcaaacg attagctata actcaaaaca
    32101 ttcaatttga ttttgtaaaa gataaaaaat ataacaaaga tgccctagtt gttaaaatgc
    32161 aaggattcat cagctctaga acaacatatt cagacttaaa aaaatatcca tatattaaaa
    32221 gaatgatatg gccatttcaa tataatatca gtttgaaaac gaaagactct aatgttgatt
    32281 taatcaatta tcttcctaaa aataaaattg attcagcaga tgttagtcag aaattaggct
    32341 ataatatcgg cggaaacttc caatcagcac catcaatcgg aggcagtggc tcattcaact
    32401 actctaaaac aattagttat aatcaaaaaa actatgttac tgaagtagaa agtcagaact
    32461 ctaaaggtgt taaatgggga gtgaaagcaa attcattcgt tacaccgaat ggtcaagtat
    32521 ctgcatatga tcaatactta tttgcacaag acccaactgg tccagcagca agagactatt
    32581 tcgtcccaga taatcaacta cctcctttaa ttcaaagtgg ctttaatcca tcatttatta
    32641 caacattgtc acacgaaaaa ggtaaaggtg ataaaagcga gtttgaaatc acttacggca
    32701 gaaacatgga tgctacatat gcatacgtga caagacatcg tttagccgtt gatagaaaac
    32761 atgatgcttt taaaaaccga aacgttacag ttaaatatga agtgaactgg aaaacacatg
    32821 aagtaaaaat taaaagcatc acacctaagt aaacagttca atcattttat aaaatcctgg
    32881 gacacttcat acttgtctca ggatttttta acatattgaa tcagcctcat aacattaaat
    32941 tatttaatcg tacattaaat ttaataataa caactgattt ttataagaac aaagtatcgc
    33001 atcatagtag atacacaaat aatacaaatg aagcaattta acttgaaagc ttaaataaat
    33061 attctcaagt taataaataa ttaactttta gatggattca tcaaaaatcg caaaaaagaa
    33121 caatttgtat tttacaaaca ttaattaaaa ataaaagcaa gacattcgtg caatcggttg
    33181 ccttaaaatg tttacaactg tcaacaatac caaggtttta ttaactatat ttctcacaaa
    33241 attagctttt agcattccaa acaaaaaagg ttaaattgaa cggaattatg gcatttttaa
    33301 cttaattgta aaaaaagttg ataatggtca attgttaatg aacagttaat tataataacg
    33361 ttcaaaatat attattattt aattaagtta aataaaatta tagaaagaaa gtgaaactta
    33421 tgcttaaaaa taaaatatta gctacaactt tatctgtaag cttacttgcc cctcttgcca
    33481 atccgttatt agaaaatgct aaagctgcca atgatactga agacatcggt aaaggaaacg
    33541 atgtagaaat tatcaaaagg acagaagata aaacaagtaa taaatggggc gtgactcaaa
    33601 atatccaatt cgactttgtg aaggataaaa aatataacaa agatgctttg atcttaaaga
    33661 tgcaaggatt cattagctct cgaacaacat attataacta taaaaataca aatcatatta
    33721 aatctatgcg ttggccattc caatataata ttggtttgaa aacaaatgat aaatatgttt
    33781 ctttaatcaa ttatttaccg aaaaataaaa ttgaatctac aaacgtaagt cagacattag
    33841 gatacaatat tggtggtaat ttccaatcag ccccatcact tggtggtaat ggatcattta
    33901 actattctaa atcgattagc tatacacaac aaaattatgt aagtgaagta gaacaacaaa
    33961 actcaaaaag tgttttatgg ggcgtcaaag cgaattcatt cgccactgaa tcaggtcaaa
    34021 aatcagcatt tgatagcgat ttatttgtag gctacaaacc tcatagtaaa gatcctagag
    34081 attatttcgt tccagacagt gagttaccac ctcttgtaca aagtggattt aacccttcat
    34141 ttatcgcaac agtatctcac gaaaaaggtt caagcgacac gagcgaattt gaaatcactt
    34201 atggaagaaa tatggatgtc actcatgcca ttaaaaggtc aacacattat ggtaacagtt
    34261 atttagatgg tcacagagtc cataatgcat ttaaaaatag aaactacact gtgaaatatg
    34321 aagtcaattg gaagactcac gaaatcaaag tgaaaggaca gaattgatat gaacatgaat
    34381 aaattagtca aatcatccgt tgctacgtct atggcattat tattactttc taatactgca
    34441 aatgctgaag gcaaaatcac accagttagc gtcaaaaaag tagatgataa agttacttta
    34501 tacaaaacta cagctacagc agattcagat aaattcaaaa tttcacagat tttaacattc
    34561 aatttcatca aagataaaag ttatgataaa gatactttag tgcttaaagc tgcaggtaat
    34621 attaactcag gttatgaaag accaaatcct aaagactacg acttttcaaa aatatattgg
    34681 ggtgcaaaat acaatgtgtc tatcagttca caatctaatg attcggttaa cgtagtagac
    34741 tatgcaccta aaaatcaaaa tgaagaattt caagttcaaa acactttagg atatacattc
    34801 ggtggtgaca tcagtatctc taatggttta tctggcggac ttaacggaaa cactgctttt
    34861 tctgaaacaa taaattataa acaagaaagt tacagaacaa cattaagtcg caacacaaat
    34921 tataaaaatg ttggctgggg agttgaagca cataaaatta tgaataatgg ttggggacct
    34981 tatggaagag atagcttcca cccaacatat ggtaatgaac tcttcttagc tggcagacaa
    35041 agcagtgcat acgctggcca aaacttcata gcgcaacacc aaatgccatt attatctaga
    35101 agtaacttca atccagaatt tttaagcgta ctatcacaca gacaagatgg cgctaaaaaa
    35161 tctaaaatta cagtaactta tcaacgtgaa atggatttat accaaattcg ttggaatggc
    35221 ttctactggg caggcgcaaa ttataaaaac tttaaaacta gaacatttaa atcaacatat
    35281 gaaattgatt gggaaaatca caaagtgaaa ttgttagata caaaagaaac tgaaaacaat
    35341 aaatagctag taaaacacgg tcgccaacag taattgtgac gaccgtgttt tgatttatta
    35401 tcttagtaag actgccattc tttttctcaa ggtgagatat aaaggaatag ctacaattaa
    35461 agtgaatatt acgcctggaa tcgcgtttaa caacactacc cacacaggta aatttaaaat
    35521 aatagataat agtagcctag ataccaaact gcctaataca cttgctaaaa ctaatgatag
    35581 tacatttatt ttcaataaat aaacaactgc aatagctata actctaaata taatagaaat
    35641 aatcacatta attggattaa atacgccaaa tatcagtaat aatacgcttg ataaaagccc
    35701 acctaaaaag tactttttaa ttccaaagaa agctaatatc aataatgctg ctggtgcaga
    35761 taattgaaaa tctaatcctg gtataatgga tggtattttc aaaactgcca aaatggttaa
    35821 aatcgcagca atgacactaa tttgagtaat atcttttgat gtcatactaa aacccctata
    35881 ccgtttcata aataacttgc ttcggtgtgc tttctaaaaa tgatatgtaa tgatttaaat
    35941 caatacgatc gtccacaaat attattctgc ctccatatct cgtattaact ggtttaatat
    36001 caaataatcg atggtaacca attttagcag aggcaaaata acctgtcgta tacgttaagt
    36061 catcggacac gcaaagttct cctttgacat acggatgcgc attgatacaa ctagcaattg
    36121 ctaaggcatc agtcactctt tcattaagat cacctttatt atttatatct tcaaacgaaa
    36181 aatgtgttgc cctaatcccc ctttgtccaa atgaatctaa acgtttacca gatatagcag
    36241 atagaataat agctcctgta taaaccgttt catttttaat atatgtcatc ccttgattta
    36301 gcgcttgttc agttacacca cattcttgtg ttaaatgttg gagatttgct ttatcatcat
    36361 caataatttg taatgctttt atttgttgaa tcggttccat gattttttgt atttttatat
    36421 tgagaaaatc cggttgccca ttttcatgaa aaaaaccttt attataaaac ctttgtaccg
    36481 tctgttctat tttgtcaaac tcacaaatcg tttcagcacc actaatatga acatcttgat
    36541 tgctagaacg catttttata ctatacatga cgatcacctc aatcttcttg atgcaaaatt
    36601 tcaaacaacc tatctatatc ttgttcagca tgaaaatacg acaatgatat tcttaacatt
    36661 ggcttagtca cagttggata ccttaaataa cttgtaaaca catgatgctt taataatgtt
    36721 tgatgaatgt tctcagccgc ttctatgtca tcaaactcaa taaacttaat cggcgagttt
    36781 gaactattat aattaacatt gagtgctttt aacttttggt taaaatattt actcaaacta
    36841 tttaatttag tgcgtctatc atcagcattt attaactttt caatatttct ttttataaaa
    36901 tacaaattat aaattggcaa actacttgag tagatgagtg gtctaccgtg attaattagc
    36961 atatccttca catcatttga acttaaaatc acacccccgt atgcaccaca tgctttagat
    37021 aaactagaag tgagtatatc tatgccttga taattcgagt aattctctat tccaaaacta
    37081 tgtgaaacat cgagtatcag tgttgcgtta tatttatgct ttaatgagac taattgacca
    37141 atatccacaa cgtcgccatt cgttgaaaat acactatcag atatgattat ttttggtata
    37201 ttttgattag ggtattccgc taaccttttt tctaaatcag caatatctaa atgcttatat
    37261 atcacttttt ctaaaccact tagcttaata ccgtcaataa tactcgcatg attttcttga
    37321 tctgaaaata cgacacaatt tgtatttttg aaaatattaa ataacgccaa attagcatca
    37381 taaccactat ttaagatagt acatgcacta tatccgagcc agtctgctaa cattgattca
    37441 atctcttcat aaactgtcga acttccacta atcaatcttg aacttgataa gtgataacta
    37501 tacttccgca taaaactttc gaaatcgact tgatcaaacg ctatttgacc taatcctaaa
    37561 taatcattag atgtatagtt cgtacatcgc ttattttcta cttcaatata ctgtctatct
    37621 atatacccta ccgatttaag cgaccgatat aaccctttct gttgtaataa atcaatttgc
    37681 tcttgaaaca tcattcttgt tttccttatt ttcacaagtg ccataatcaa tttcaaagcc
    37741 caaatcatta atcatatcgt agtctaattg gttcggttgt ccaccagtaa ttagataatc
    37801 accgacaaat attgaattcg ccgcttttaa tgctaatggc tgtaacgaac gtaagttgac
    37861 ttctcttcct ccagcaatac gaatttcttt cgtaggattg attaatcgga ataaagctac
    37921 aattcttaaa catttcattg gtgttaaatc atccatgcta ccgaactttg tacctttaat
    37981 tggatgtaaa aagttaatcg gaatactgtc ggcatccatt tcttttaaag caaatgccat
    38041 atcaacaata tcttgattag attctcccat accacaaatc acgcctgaac atggtgatat
    38101 attattcgct ttcattagtt ctatcgtatc tgttctatct ttataactat gcgttgtcac
    38161 gacgttatca tggtaatttt cacttgtatt aatattgtgg ttatatctgt ctacaccagc
    38221 tgacttaagt ttcttagctt gttcgtcatt cgttaatcct aaacatgcac atattttcag
    38281 ttgcgggtgt tgagatttaa tcgttcttac agtgttacta atatgatcaa cttctttatc
    38341 actcggccct ctaccactca taacaataca atatgttcca atatgattat catgtgccac
    38401 ctttgctcca tcgataattt gttcctctgg aattaaagca tatcgctgtt tttgtttaat
    38461 atctcgtgat tgtccacagt acccacaatt ctcaggacat ataccacttt tagcatttaa
    38521 aatcatgttt aattttactt ttttaccgaa ataatgtttt cttaaaatat acgcctcatt
    38581 taataaatct aaggtatcaa tattagtatc ctcataaatt ttcaatacag tctcttttgt
    38641 taattgttcc ccttgtaata tgcgtttagc caaattcata ttaacacttc ctatctaaaa
    38701 tgttatgtac tgccttttta aaaatacgta ccatcgtttt cacttcttta tttgacatac
    38761 ttaacactgg cacaaacgta atgacatttt ctaaatttct aatcattagc ccgttttctt
    38821 tacaattacg tacaatacgt tcaacttttt caatttctaa cggcgtttta ctatctttat
    38881 ctgtcactaa ttcaactcca aacattaagc ctcgtcctct tacatcacca acatttcgat
    38941 gacgcttcag cgcatgtaac tgtttctcaa gtgttgatga tgtcgtttca atatgtgaca
    39001 ataacttacg tttttcatat agtcttatat tttctaaagc aaccgtacaa acgatttgat
    39061 ttccggtgta tgtatgtcca tggaaaaagg tattcacacc atgcgaatca cttaaaaatg
    39121 cattgtatat tttttgagat gtcaatgtag ctgcaagtgg taagtagcca cctgtaatcg
    39181 ccttacctaa acacataata tccggttgaa catcttcatg attgcatgca aacatctttc
    39241 cagttctccc aaaaccaact gctacctcat cacaaattaa taagacatcg tattttttgc
    39301 acaatttctc gacttctttc aaaaagcctt taggatgaac aaacaagcct gtcgcacctt
    39361 gaatcaacgg ttccaatata aaccctgcga tttcatcatt tctttcaatt agaatttgct
    39421 ctatattcgt taaaatagct gccatcattt cattttcagt atcgtaatta ctgcgataga
    39481 aagatgggct ttctacttga atattctcaa atattaaatc tttaaatatt ttatgaaagg
    39541 tcttgatacc accaacactt accgcaccaa tcgtatcccc atgataaccg tgatttagcg
    39601 ttataaactt gtttttcttg gcatattttt ctctatcaat atttttccaa tactgatatg
    39661 ccatctttat tgcgatttca acagacgcac tgcctgtatc agaataaaat acttttctta
    39721 gattacttgg cgtgatttcg attaattttt ccgcaagttc tattgacgga atatttgata
    39781 atcctagcag cgtagaatgg gcaattttat tgagttgctt tttaattacc ttattcaagt
    39841 atttgttatt atgaccatgc acattgaccc acaacgatgc ataaccatct aaatatttat
    39901 tgccattcgt atcgtaaagg taactaccct ttcctttttc aatgatgatt gcttcttctt
    39961 tgctatatac acccatttgt gtaaatggat gccaaacata ttctgagtct ttttgtttaa
    40021 gttgttgtgt gtaattcatt tttaactcct attaattgct ttaaaaattc ttctgagaaa
    40081 ttttcatacg tggcatgttc ttcaaatgta tagactgttt tatttgttaa ttttccaatc
    40141 gtcatttgat tgtctttttc aatataactg tctgtatagc gattcatgat taaaaaattt
    40201 ctcgccgata cattatgatt aacataatct tggtgaacaa tggcatcgct aatagcacct
    40261 aactttgatg gcaacacact gatgacacaa tctgcacaat cattgattaa atctttagtc
    40321 atatagaaat catctgtacc ttcatatatt ggtacgccaa ttcccccagc accctcaatt
    40381 aagataaaat caaattcctt atctaaaact tttaccttat ctaatacaca ctgcttattt
    40441 agaaaaattt gatctgccat tttaaatgca aggtgtggtg atacaggttg cttaaaagta
    40501 taaagtgacg ttatgtcata gcttaaatca cattcatttt taaatacttc taaatctgga
    40561 aacgtcccgt cttggcgttc ctcagtttga aatggtttaa aaacacaaac acgatgacca
    40621 cgtgttttca aagcatggta taaatgcttt gtaacatagg ttttgcctac atcagtattc
    40681 gtacttgtaa taaaaatcct cataaaaaat aagcacctca atccaaatgt aaacttttat
    40741 aattaataag tttacattta aggcgcatgt ttttcaagaa aacatgaatt taaacttccc
    40801 agtataacta taaattttgg caaaattttg accttgctat tgaaatgact aaggcttctt
    40861 acggaattcg ggcagatata aacagcgatt cgataaaa
//
LOCUS       DAPIDM010000010        38119 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000010 DAPIDM010000000
VERSION     DAPIDM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.010"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            118..312
                     /locus_tag="RJE42_000440"
     CDS             118..312
                     /locus_tag="RJE42_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875893.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDAPKFGCVNGCMLFILY
                     YRVNRIGENRSFEISTNPIF"
     gene            complement(381..1190)
                     /locus_tag="RJE42_000441"
     CDS             complement(381..1190)
                     /locus_tag="RJE42_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5875894.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYSYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            1800..3755
                     /locus_tag="RJE42_000442"
     CDS             1800..3755
                     /locus_tag="RJE42_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5875895.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTLR
                     ILIKYLIYSKIKQQPYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGDTPKLIRKRNSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPNELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3781..4524
                     /locus_tag="RJE42_000443"
     CDS             3781..4524
                     /locus_tag="RJE42_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDV5875896.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKARDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLLNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            4588..4914
                     /locus_tag="RJE42_000444"
     CDS             4588..4914
                     /locus_tag="RJE42_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDV5875897.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGKNEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            5342..5689
                     /gene="sarR"
                     /locus_tag="RJE42_000445"
     CDS             5342..5689
                     /gene="sarR"
                     /locus_tag="RJE42_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDV5875898.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(5878..6714)
                     /locus_tag="RJE42_000446"
     CDS             complement(5878..6714)
                     /locus_tag="RJE42_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDV5875899.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSIKEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(6714..7328)
                     /gene="ureG"
                     /locus_tag="RJE42_000447"
     CDS             complement(6714..7328)
                     /gene="ureG"
                     /locus_tag="RJE42_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDV5875900.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKHPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(7341..8030)
                     /locus_tag="RJE42_000448"
     CDS             complement(7341..8030)
                     /locus_tag="RJE42_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDV5875901.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHYLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(8023..8475)
                     /gene="ureE"
                     /locus_tag="RJE42_000449"
     CDS             complement(8023..8475)
                     /gene="ureE"
                     /locus_tag="RJE42_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDV5875902.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(8488..10203)
                     /gene="ureC"
                     /locus_tag="RJE42_000450"
     CDS             complement(8488..10203)
                     /gene="ureC"
                     /locus_tag="RJE42_000450"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDV5875903.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDYEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(10200..10610)
                     /locus_tag="RJE42_000451"
     CDS             complement(10200..10610)
                     /locus_tag="RJE42_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDV5875904.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFERELAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGDENVNKKGGKRS"
     gene            complement(10624..10926)
                     /locus_tag="RJE42_000452"
     CDS             complement(10624..10926)
                     /locus_tag="RJE42_000452"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDV5875905.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            11186..12091
                     /gene="yut"
                     /locus_tag="RJE42_000453"
     CDS             11186..12091
                     /gene="yut"
                     /locus_tag="RJE42_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDV5875906.1"
                     /translation="MKMIDVLLKNISQVVLISNKWTGLFILIGLFVANWTVGLAAMIG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHVIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKNDKVDA"
     gene            12308..13462
                     /locus_tag="RJE42_000454"
     CDS             12308..13462
                     /locus_tag="RJE42_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDV5875907.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIGDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKEYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELKSVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNSTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            13675..14583
                     /locus_tag="RJE42_000455"
     CDS             13675..14583
                     /locus_tag="RJE42_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5875908.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            14982..15923
                     /locus_tag="RJE42_000456"
     CDS             14982..15923
                     /locus_tag="RJE42_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDV5875909.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDTSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            16044..16598
                     /locus_tag="RJE42_000457"
     CDS             16044..16598
                     /locus_tag="RJE42_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDV5875910.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            16670..17440
                     /locus_tag="RJE42_000458"
     CDS             16670..17440
                     /locus_tag="RJE42_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5875911.1"
                     /translation="MASISMSDINCDGTIFENNDKQLIYLTPIFPQRYTSNTWLYKKM
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTMQSLVGEYALSRNH
                     KPVILVADGEDTAKDMYVKQGYVYQSFCYQILKENIEI"
     gene            complement(17616..18413)
                     /gene="fdhD"
                     /locus_tag="RJE42_000459"
     CDS             complement(17616..18413)
                     /gene="fdhD"
                     /locus_tag="RJE42_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV5875912.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAVAKTSMSKITLTPQQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            18703..19485
                     /gene="modA"
                     /locus_tag="RJE42_000460"
     CDS             18703..19485
                     /gene="modA"
                     /locus_tag="RJE42_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5875913.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNQDNKKDAADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKGNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFTA"
     gene            19499..20170
                     /gene="modB"
                     /locus_tag="RJE42_000461"
     CDS             19499..20170
                     /gene="modB"
                     /locus_tag="RJE42_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV5875914.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGVFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            20171..20776
                     /locus_tag="RJE42_000462"
     CDS             20171..20776
                     /locus_tag="RJE42_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV5875915.1"
                     /translation="MLKINLKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            20944..21948
                     /locus_tag="RJE42_000463"
     CDS             20944..21948
                     /locus_tag="RJE42_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDV5875916.1"
                     /translation="MSQERYSRQILFKQIGEIGQNKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLYTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENTSITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            21978..22484
                     /locus_tag="RJE42_000464"
     CDS             21978..22484
                     /locus_tag="RJE42_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDV5875917.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRNFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(22486..22980)
                     /gene="moaC"
                     /locus_tag="RJE42_000465"
     CDS             complement(22486..22980)
                     /gene="moaC"
                     /locus_tag="RJE42_000465"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV5875918.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIYKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFNWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASVTALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QLDQ"
     gene            23043..24302
                     /locus_tag="RJE42_000466"
     CDS             23043..24302
                     /locus_tag="RJE42_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV5875919.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSTMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            24299..24784
                     /gene="mobB"
                     /locus_tag="RJE42_000467"
     CDS             24299..24784
                     /gene="mobB"
                     /locus_tag="RJE42_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV5875920.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKAHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRDEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            24798..25244
                     /locus_tag="RJE42_000468"
     CDS             24798..25244
                     /locus_tag="RJE42_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDV5875921.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            25250..25483
                     /gene="moaD"
                     /locus_tag="RJE42_000469"
     CDS             25250..25483
                     /gene="moaD"
                     /locus_tag="RJE42_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDV5875922.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEILLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            25490..26089
                     /gene="mobA"
                     /locus_tag="RJE42_000470"
     CDS             25490..26089
                     /gene="mobA"
                     /locus_tag="RJE42_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV5875923.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAISTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDSLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            26109..27131
                     /gene="moaA"
                     /locus_tag="RJE42_000471"
     CDS             26109..27131
                     /gene="moaA"
                     /locus_tag="RJE42_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV5875924.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYAMSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATADGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(27526..27696)
                     /locus_tag="RJE42_000472"
     CDS             complement(27526..27696)
                     /locus_tag="RJE42_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875925.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDNNVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            27822..28172
                     /gene="sarV"
                     /locus_tag="RJE42_000473"
     CDS             27822..28172
                     /gene="sarV"
                     /locus_tag="RJE42_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDV5875926.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(28274..29485)
                     /locus_tag="RJE42_000474"
     CDS             complement(28274..29485)
                     /locus_tag="RJE42_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5875927.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFVICVILGILGLIISLFIKVDFEVTNSKTETNVIDKPRFSIHQFIA
                     KEAILVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVARNAMN"
     gene            complement(29478..29918)
                     /locus_tag="RJE42_000475"
     CDS             complement(29478..29918)
                     /locus_tag="RJE42_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDV5875928.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            30080..30844
                     /locus_tag="RJE42_000476"
     CDS             30080..30844
                     /locus_tag="RJE42_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5875929.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKIKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(30836..31087)
                     /locus_tag="RJE42_000477"
     CDS             complement(30836..31087)
                     /locus_tag="RJE42_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875930.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIALAVIHMGILAHSTYVYLY"
     gene            31476..32741
                     /gene="femX"
                     /locus_tag="RJE42_000478"
                     /gene_synonym="fmhB"
     CDS             31476..32741
                     /gene="femX"
                     /locus_tag="RJE42_000478"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDV5875931.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEIQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKTDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            32858..36025
                     /locus_tag="RJE42_000479"
     CDS             32858..36025
                     /locus_tag="RJE42_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDV5875932.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGNKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASASNNVSGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIVSATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(36212..36529)
                     /gene="mspA"
                     /locus_tag="RJE42_000480"
     CDS             complement(36212..36529)
                     /gene="mspA"
                     /locus_tag="RJE42_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDV5875933.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVIFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(36774..36938)
                     /locus_tag="RJE42_000481"
     CDS             complement(36774..36938)
                     /locus_tag="RJE42_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875934.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            37071..37979
                     /locus_tag="RJE42_000482"
     CDS             37071..37979
                     /locus_tag="RJE42_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDV5875935.1"
                     /translation="MTEQFTMIILLIALGYFLKRINYIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
BASE COUNT        13490 a         5410 c         6958 g        12261 t
ORIGIN      
        1 cggttataat ttgaaaaggt tttaacttag aataaatatc ctctatacat atactgaata
       61 tgttttgtag cggaacatgt taatatatgt aatgtaagtt ttatgtcatg acatgtaatg
      121 actaaattaa ttgagaattt gaaggcaagt atatttgtaa gtactttaac taaaaattta
      181 tcaatgtata gccgatttga cgcgcctaaa tttgggtgtg tcaatggctg tatgttgttt
      241 attctttatt acagagtgaa tcggattggt gaaaatcgaa gttttgagat ttccaccaat
      301 ccgatttttt gatgtctaga aattaaaaaa gccaacaagg ctcttaaaac cttgttggct
      361 taattcactt tcatcacaaa ttagtgaatg aagttataac cagcagcttg gctagctgag
      421 attgtacgtg aagttacaac acctgggcca taaccatagt tcatttctga aactcttact
      481 gaaccattgc tgttaacact ttcaacgtat gcaacgtgac cgtatgcacc ttgagttgtt
      541 tgcataattg caccagcttt tggtgtattg ttcactgtgt aaccagctct tgcagctgcg
      601 ttagcccagt tacttgcatt gccccaagtt gaaccgattt taccacctac acgatcaaat
      661 acgtagtatg tacattgacc agaagtgtat aagttacgtc ctgaagtata accacttgag
      721 attgaacggc catttgatga tggagccata gttgtagtta cttgaacatt gttgcttgaa
      781 gtgctgtagc ttgcacctaa accaccagta cggtagctgt ttgtgttgta actattatag
      841 ttgttgtagt tatatgattg attattattt gagtagttgt tgtaacggct gtagttattg
      901 tagctataac cgttattgta attgttgtag ctatagttat tgtagccatt gtagtagtaa
      961 tagctgtaat agccattatc ttggtttaat tgacttggat gccagttacc tttccatgtg
     1021 taatggtagt taccttgtgc atcaatagtg taagtatagc tatatgatgt tgggtcattt
     1081 ggattataac cgtagttatc ttgctcagaa gcatgagctt gatttcctga tgcaattgcg
     1141 attgtagcga atcctgcagt tgcgatagta gctgtagcga ttttcttcat tttaaaaata
     1201 tcctcctaaa aattttaaat ctaaaatatt ttcgtaatgt ccgtgtgaca aaattaatgt
     1261 tataagttat ctctcgtaat taaacgacaa gaaagactat aacagaaatt agcgtccttg
     1321 tgtgctttgt taacgttttg taattttttg ctaatatctt gacacaatag aattgtaaaa
     1381 gtatagaaat ttgcattttg caaaacttat aactacggca ttctttgtaa aaactgaatg
     1441 tttcgaaaat aagtctgtta caaatttgta atattactga aaattctaaa tgtatatttt
     1501 gtgcataata taggactttt aatcagaaaa tgcataaaaa ttcgacacga acgatcaaat
     1561 ttctttggtt aggaaagctt tggtatttgg ataaaaatgt atatttaatg gataaaatat
     1621 cctaatttag cataaaaaaa tgttttaata aaagtgttat ttgatataat cgatttatgt
     1681 tttgttactg ctaaaaaaca tgtggcgata taattggtag tgaatattag atgttgacaa
     1741 ttgatttgag aaagacaaac aggagtaaat caatatattt gtatatgtga ggatgtaaga
     1801 tgacagataa ttattcacta catattttta aaagtatgaa aatgagtagt gtgaaggaag
     1861 caaacttaaa aattatatat tggcttcaag gtacaggtgt agtttcaatc aatctacaac
     1921 aatatgatgt gaaaagaaat gatgtcaccg ttattatgtt gaatgatttg tatcagattg
     1981 atggtcatga tgatagtgta tgttgtgttg ttgaagtaag tgcaaagaca tttttgaagt
     2041 ttatgaatgc aaactatatg atgcgtggca agatattaac aaatgaggtt gctagcactc
     2101 ttagaatctt gattaagtat ttaatctaca gcaaaattaa acaacagcct tataatgcta
     2161 atgataagat tattaattat atgtgtgttg aattaatgtc attaaataca tacgatggta
     2221 gtcgtcattt agtagccgaa gaagtgcacg attatttaac aaacaaccac cataaaaaga
     2281 taaataggaa agatgtcatt aatcaagtta gtatcacaaa caaagcttta agtgacatgt
     2341 ttaaagcgac accttatagt aattttattc aatatttaaa tcatattaga ctggagcatt
     2401 gcttaattga tattttaact tctaagaagc ccattgaaga aatcgcaagt agtcatggtt
     2461 ttaatcatta ttctcgtttt atacacctct ttaaagaaac gtatggtgac actcctaaat
     2521 taattagaaa aagaaatagt ccaacatctc attcaattaa ccattcccaa ttagttgaaa
     2581 ttgataaaaa tataattgaa ttatttgatg atactaatca aagttcttca agtatgcgcg
     2641 agattgatat accttttgag cctacaaaaa ggagtcaaat gtatcaacct aaaaacatct
     2701 atattaaagg aagtaatttt tattggatgg attactatac tattaaacaa atcattcaaa
     2761 gattaggtgt taatcctgaa aacttgcata taattgttac aatcgataca agcaatgagc
     2821 tatcgattga acaaatcact ttattacttc aaaagatagt gaattacaat gtaaatgtag
     2881 tatttcgtat taaacatgaa tataaggaat tgttaacgag tactgaaaga ggcaagatag
     2941 aaaagctagt agctacaatt tttaatatga cttttgagag taatagatgt aagattgcat
     3001 ttttaattaa cgacttaaat actactgcaa ttcaaaaact gaaacgttta atagataatt
     3061 atataggagt atttgaatta atatatcatc tggaaccaaa tgagttgaat acgcgctatg
     3121 ataaagaaat tgatcatcta atagactatt ttatcttacc gctaagacaa atagaacagg
     3181 tgtctattgc acgttctaaa attatatgtg atgtagattt gattaatgag aataatgact
     3241 taaaagaaaa aatcacaatg tctgatatta ataaggttac tgaacttttg acaaattata
     3301 caaaatttcg aggacttatt atgcacatgt caattataag tgaagcgagc aaagaaataa
     3361 atttgactag attatcaata ttcattttta tcataaatat acttaatcaa ttacgaggga
     3421 ttgttattta tcaaaatgac acgatgattg taacgaagtt caaacatgaa gtacaatgtg
     3481 tcatttgttt gccagccgca ttgttaaatg agaattcaga cgctatacgt ttatcgtgta
     3541 agggtcttca acaatttagc catgctaaaa ctaaacaaat tgttttaagt atagataaag
     3601 ataatcatac ttccgaaaat ggtgtacttg tgaatgatat tatgcaacaa cacagttatt
     3661 ggaaagctaa ttttatagaa agaaatattt atgatcattc attaatggtc tcaagcaata
     3721 gtattgttca tttgaaatat catcttgaga tataggcatg gtttaagaga ggttttaaag
     3781 atgtctaaat ttaaagttaa gcgtttaact gcacacatac taatgttgaa agtcattaat
     3841 gatatgttga aatattcatt tcatctcact ttgactcaag tgaaactatt aaatctatta
     3901 gttaaatatg ataattctga ttccaatagc gcttcaatag ataggttatt aaaaatgaaa
     3961 gaaatccaat ctaagatggc acttcttcaa atgttaagtt atttaaacaa tcatcaatgg
     4021 ctattgaaag caagagatca aagggatcaa cgtaaattga ctatttcgat taataaaatg
     4081 catatagata aaatagaata tatgaataat gagcttagtg actatattga acgttatttt
     4141 ggtcaatata catttgattt tagttgtgac tatgtatcgt atttattgtc gtcacaagaa
     4201 ttattgctaa atctaaagtg ttatttaaat atgtgtcaat tatcattaga agagttatac
     4261 gttttaggaa tattaaatct gcataaaggt caattgacgg ttaaagaatt acagggtgaa
     4321 ttccatcacc cgatatttgc ggttagtccg attttaaagt gtttaatttt gaaaggatta
     4381 gttaaaaaag agagatgtga attagatgaa aggcgtgtta ttgtaacaat caagcgagaa
     4441 aagttttcta aagtcactat gcttattcaa gcatgttata attatcttga aaagggaata
     4501 caagttaagc ttaataataa atagttaatc ttttgtattt catataccta tgaagaatag
     4561 tttatagttt taagtgggag ggatattatg attttagata atgtaaatcc aaatgattta
     4621 tttccaactg aaaaaaaggg cccgtcagtg ctaggaataa ttgagtatca agttcaaggt
     4681 aagaatgaat ttgaaggggc atttatagca acaaatgaaa gattaattat gaatgtagat
     4741 atgaatggtc aattttatta tagaagtatt agttataatg aaatagagaa gatagattat
     4801 gatggtcaaa ctataatgtt caaatttaat attggtaatg tcccaatgca tgatattaaa
     4861 agtgataacg ttgaaatgtt tgttgaatac gtaaaacaac atatgatagt ataatgtcaa
     4921 tgtatgtagg ttgataagaa gagtttgaat gataactcat tgcatttaaa gataaatggc
     4981 atgcgttatg atttaaagct cttctttttt tatattttat aaattttgtg agcaagccat
     5041 caaattaata taaaaaatat caaatcaaac acgttgatat agattaaaat tttaaaagtt
     5101 tacatatcag ttaagataca aaatattcag actaataatt ttcaatttgg caaaatatct
     5161 taaacatcaa attattataa gaaataaatg tatttaacca tattctagta ataaaaatat
     5221 tgaattttta tacttatttg tttagaatga actttataac atagttggat agagtttcga
     5281 tttaataaat tacatgtgaa ccttgctaca acaagatgtg catcagagga gtggtttaat
     5341 aatgagtaaa attaatgaca ttaatgattt agtcaacgca acatttcaag ttaagaagtt
     5401 tttcagagat acaaaaaaga agttcaattt gaactatgaa gaaatttata ttttaaatca
     5461 tattttaaga agtgagtcta acgaaatctc atctaaagag attgctaagt gctcagagtt
     5521 caaaccttac tatttaacta aagctttaca aaagctaaaa gatttaaaat tgttatcaaa
     5581 gaaaagaagt ttacaagacg aaagaacagt tattgtttat gttacagata cacaaaaagc
     5641 aaatattcaa aaactgattt cagaattaga agaatacatt aaaaattaaa tcaaggttaa
     5701 ttgcgtttaa taacattgaa cgataacaat ttattaaaac gaagttattt attcagcatt
     5761 gggacataaa attaacttaa aatttaaata ttgaagatgc tttaattaaa gttaaagact
     5821 agccatacct tatttcagct tattaagctt gaaacaaggt acactagtct ttttatttta
     5881 atattttctt agaaaatcaa gtttacgatc ataaatattt tctgcgatat agctttggat
     5941 ggttccaagt attttctcta taatttgtgt gcgataagca aaaattctaa ctgcaaaacc
     6001 atgtgtaggc aattgagaaa tagcaacacg acaatcgaat gtattgctat aagaactaat
     6061 ggtttcataa actgaatcga ttagtttttg gttaacatct tcatgtatga agtaagcaga
     6121 gccataatga gagtaatgtt ccatataacc tatttctttg atcgattgtt tattagggtt
     6181 caataataaa ttatcatatg tgactaactc atcttcaata taaatttcat ttattaaatg
     6241 catatattga tacttgaagg cttcgccagt ttttgaataa ccaggagtta aaatatcagt
     6301 ataaaataat gaactagaat tattgagatt gaacgtattg tgttgataaa atttagcatt
     6361 ttcatatgca atgattggat cagcgacata ttctaaatat gcgttatctt tcaaattaaa
     6421 agtttgatac tgctcaacat gattggaagg tgtcttgtat atttttgttg caccttgaga
     6481 tgtcaatgtc actttagcat tatcttcgac attcacattc atacggtaac gatctccatc
     6541 taaatagcca ccacctacat ttactatata aaatgtagga atagtagatt gatttagata
     6601 aactggacgt atcactttaa gtgctttttc aaagaaaata tctcttgata ctgatcgatt
     6661 gccatcaaaa aacactgtta aatcaagttg cccagtccat tgttgttcat ccattatgat
     6721 aatcctttga gtaaagtgtc gcgttcaatc caatcaataa cttcatccaa accttcgtcg
     6781 gtttttaagt tagtaaaagt aaatggatgt ttaccacgaa atactttagt atcttcagcc
     6841 atttgttcta atgatgcacc aacataggga gctaaatcag ttttgttaat tacaaagaaa
     6901 tctgacttaa tcataccttg accaccttta cgtggaatct tttcaccttg agcaacatcg
     6961 ataatataaa ttgaaaagtc aacaagttct ggactaaatg ttgctgctaa gttatcgcca
     7021 ccagattcta tgaaaattag ttctatatcg tcatgacgtt ctaataattc gtctattgct
     7081 gcaaagttca tagatgcatc ttcacgaatc gcagtatgag gacatccacc agtttcaaca
     7141 ccaatgatac gactttcagg tagaactcct gaatttacta atatcttttc gtcttctttt
     7201 gtatatatat catttgtaat aacgccgata ctcatttctt ttgaaagacg ttttacaact
     7261 ttttcaatta attgtgtttt gcctgcacct acaggaccac caataccaat tttaatcgga
     7321 tttgccacaa ttataacctc ctatgatatg aaaattctaa cattgacgtt ctcatgcgcc
     7381 atttgattta gttctaaacc aggcgctgtc atgccaaaat ctgcttcttt taattcgaaa
     7441 atctgctttc ttgtttcttc tatataagga atcatgtgag taactatctt ttgaccagca
     7501 gtttgtccaa gtggaatagc acgaacagca ttttgagtta aacttgaaac attttgatat
     7561 aaatagtaat caataatcgt ttcaatatct acacctaaat aatggcctag catagtaaaa
     7621 caaatagctg gatttaactt tgctttctta tcttgcattt gttgatgata ccaagcaatc
     7681 catgggctat tataaagttc taaagccaat ttaaccatgc gagtccccat ttgttttgca
     7741 ccaacacgtg tttctttagg taagttttga acaaacatta gcttatctat gtgtaatact
     7801 ttttgtgtat catcattttc caatgcatca taaactaaac gcatggctaa accatcagaa
     7861 taggtaagtt gctcttgtaa aaacattttt aaccaagcaa taaaagtatg atcatcatga
     7921 attatatttc gttgaatata tgtttcaaga ccaaatgaat gactgaaagc acctgttgga
     7981 aactgtgaat cacagaactg aaataatctt aagtgtgtat gatcaatcat gagaatgtcc
     8041 tatatgtctg aaagccttat taactttacg gtcttctcta acatatggga tgcctaaact
     8101 ttttaataaa tcttcaacta aataatcata ttgtactagc atttcagtct ctgtaaattg
     8161 tgctggcaaa tgacgatttc ctaattgatg ggctatatct cccatttctt gcaatgttct
     8221 tggttgaatc actaaaagat cttctgaatt aacatccaca ataatcatat tatggtcatc
     8281 tgcgtataaa atatctccat attgtaagtc aataggttgt tttaaacgaa tgccaatttc
     8341 agtgccatga tctgttacga ctctttgaat acgtttaacg agatctgaat tttcaaggta
     8401 aactttttcg acgtgctttt gtttttctga atttgacaaa ttggcaatat tgccttggat
     8461 ttcttcaaca atcattctat gttcctccta gaataagaag tatctttgag ttaatggtaa
     8521 ctcagttgct gcattacttg taattttttc tccatctaca tatacttcat atgtttgtgg
     8581 atcaacgtct aatttaggtg ttgcactgtt atttttcata tctgctttag ataattgtct
     8641 aatattttta actggacgca ccatgcgttt taaatttaat gcacgattga taccattttc
     8701 ataagcagtt ttagacacga atgtcattga cgtacttgta aggtttccgc cgtattgacc
     8761 atacatttta cggtacttca tcggttcaga tgtaggtata gaaccatttg catcgccatt
     8821 tacggcagag ttaattagtc cgccctttac aactaattca ggtttaacac caaagaaaat
     8881 tggatcccat aagacaatgt cagctagttt gcccggctcg atagatccta catattcaga
     8941 tataccatgt gtaattgctg ggttaattgt atatttagcg atataacgtt tgatacgatt
     9001 attatcatta tgttcataat ctccatctaa aggaccacgt tgttctttca tgcgatgtgc
     9061 tacttgccat gttcgtgtaa ttacttcacc tacacggccc attgcctgtg aatcggaact
     9121 aatcatactg aatacaccca tatcttgcag aacatcttct gctgcaatcg tttctttacg
     9181 aatacgtgaa tctgcgaatg cgatatcttc aggaatagcc gcatttaaat ggtgagtaat
     9241 cattaccata tctaaatgtt catctacagt attatgtgta taaggcaaag ttggatttgt
     9301 agatgaaggt aaaatatttg aaaatgcagc ggatttaatt aaatcaggcg catgaccgcc
     9361 accagcacct tcagtatggt acatatgaag tacacggtct ttaacagcag ccattgtgtc
     9421 ttccataaat cctgcttcat ttaaagtatc tgcatgtaat gcaatttgaa catcaaattc
     9481 atcagcgaca tctaatgcat gactcaaagc agatggtgtt gcaccccagt cttcatgtac
     9541 ttttaatcca attgctccgg cattgatttg ttcaatgagt gcagttggat ttgttgcttg
     9601 tcctttacct gtaaaaccga cattaatcgg taaaccttcg gcagcttcta acattctatg
     9661 aatatgccat ggacctggag ttacagtagt tgctttagaa ccttctgaag caccagtacc
     9721 accaccaata tgagtcgtaa taccactttc taatgcgacc tctgcttgtt caggattaat
     9781 aaaatgaaca tgagtatcga taccaccagc agtgacgatt ttaccttcag cggcaatgat
     9841 atctgttgtt gaacctataa taatgtcgac attatccatt atatctgggt tgccggcatt
     9901 acctatggcg aaaatataac catttttaat gcctatatca gctttaacca ctttatcgta
     9961 atcgataata acggcattag aaatgacaag gtctgcaacg ttcacgtcat cacgtgttac
    10021 acgaggattt tgcgccatac cgtctctaat agatttacca ccaccaaaag tagcttcttc
    10081 accataaacc gcgtagtctt tttctatttg agcaaataga ttcgtatcac ctaaacgaat
    10141 agaatctcca acagtaggac cgtataagct cgtatattga ttttgcgtca ttttaaagct
    10201 catgatcttt ttcctccttt tttattcacg ttttcatcac cgttatctcc gaatacacct
    10261 gcatattcat cattttcatc agttgggcga tagacacgtg actcatcgat aggaccattg
    10321 accataccac gaaaaccaaa aattttacgt ttgccagcat attcaactaa ttgaacttct
    10381 tttttatctc caggttcaaa tcgaacagct gctccagctg gaatatctaa atgttttcca
    10441 tatgccagtt cacgttcgaa atctaatgct gcattagctt cataaaaatg aaaatgtgag
    10501 cccacttgaa ttggtcgatc tcctgtattt tcaacttcga taactgtttc aggatgatgg
    10561 ttattaattt caacctctgt actttttgta ataatttctc ctggtatcat ttgactgcct
    10621 cctttaaaca atagggtgat gtactgtgat taacttagta ccatcgggga acgtagcctc
    10681 gatttcgata tctgtaatca tgtgttcgac accatccatg acatcttctt tgtttagaat
    10741 ttgtctacca taactcatta actctgcaac ggtcttacca tcgcgtgcac cttctaataa
    10801 ttcatcgctg attaaagcta atgcctcagg atgatttagt ttcaaaccac gtgctttacg
    10861 acgacgtgca acttccgccg ccactacaat cattaatttg tcttgctctc gttgtgtaaa
    10921 atgcaaatta aaacccccaa tttcatatta gatacaattt acaaaattta tattaatcct
    10981 aattgttgtg ataaacaagt aatatacaaa gtttaatgcg gaacagtaaa aaataaattt
    11041 actatttcgt atttaaaaat atatgattac gattaaaaca aatagtaaaa gatatttagg
    11101 gtttaggaaa tagagttttt aatataactt tttgtaaaat agtagtatat taatgagtaa
    11161 acattcaaga aaaagggtgg gcattttgaa aatgatagat gtgcttttga aaaatatatc
    11221 tcaagtagtg ttaattagta ataagtggac tggtttattt atcttgattg gattattcgt
    11281 ggccaactgg acagttggat tagcagctat gataggtagt atcatagctt atgcatttgc
    11341 acgttacatt aattatagtg aagcagagat taatgatggg ttagccggat ttaatccagt
    11401 gttaactgcc attgcgttaa caatcttttt agataagtca ggattagata ttgttataac
    11461 aatgatagca actttattaa cgttaccagt cgctgctgca gtgagagaag ttttaagacc
    11521 atataaagtt ccgatgctga cgatgccttt tgtcattgtg acttggttta caattttact
    11581 ttcaggacag gttaaatttg tagatacatc gttaaagtta atgcctcaaa acattgaaac
    11641 ggttaatttt agcaacaatg atcgaataca tgtcattcag tcattatttg agggattcag
    11701 tcaagtattt atcgaagcga gtgtaattgg tggcgtatgt attttaatcg gcatattgat
    11761 agcatcaaga aaagcagcac tcttagctgt tatagctagt ttgttaagct ttatcattgt
    11821 agctctatta ggtggtaatt atgatgatat taatcagggg ttgttcggtt ataactttgt
    11881 attaatggca atcgcactag gatatacatt taaaacagcg attaaccctt atatttcgac
    11941 ttttttaggt gtgttattaa ctgtagtggt gcaactaggt acaacaacat tgcttgaacc
    12001 gtttggctta cctgcattaa cattgccatt tattattgtg acatggattt tattatttgc
    12061 tggtattaaa aatgacaaag tagatgcttg atagttaaat caaacctagt attgtttgaa
    12121 tatcacctta aaccatacag cgaattgtat agtttaaggt gtatttttat ggagaaaatt
    12181 aagtgcatac ttaatcgaat tatcagcttt cttttaattg ttcacagcac gttccttttt
    12241 atttttaaag tcgagtgtta aactaggaat aaataattta tattgtgtgt tgtgtggggt
    12301 ggctaatatg aatgatatgg ataattcctt tttaataaca acggaaattc aaagaaaatg
    12361 gattgaaaaa ttcaaagtaa ttggagatac atttaaggct aaggctgaat ataatgatca
    12421 acatagccaa tttccatata aaaatattga atggttaatt aaagaaggtt atggaaaatt
    12481 aacgttacca aaagaatatg gtggtgaagg tgcgaccata gaagacatgg ttattttgca
    12541 atcattttta ggcgaacttg atggtgccac agcattatct attggttggc atgtgagtgt
    12601 cgtaggacaa atttatgaac agaaattatg gtctcaagat atgttggagc aatttgctgt
    12661 tgaaattaat aatggtgcat tagttaatag agcagttagt gaagctgaaa tgggtagtcc
    12721 aacaagaggg ggaagaccaa gtacacatgc tgttaaagct gatgatgggt atattttaaa
    12781 tggtgtgaag acatatacat caatgagtaa agcactaaca catattattg ttgctgctta
    12841 tatagaagaa ttaaaaagtg ttggtttttt cttagtggac aggaatttgc ctggtgttga
    12901 aattgccgat aattgggacg tattaggtat gcgagcgaca gaaagtcatg atttaatatt
    12961 aaatgatgta aaagtaccat taaaacattt agtggaaaca gagaaaagta aagcgccaaa
    13021 tggttggata ttgcatatac caagctgtta tttaggtata gcacaagctg ctagaaatta
    13081 tgctgtagac tttgcaatcc aacatagccc taatagtatt gaaggaacga ttgcaacatt
    13141 gccaactgtg caacaaaatt taggaaaaat ggaaacgctg ttgttatgtg cgagacaatt
    13201 tttatggagt acagcaaaag gctatcaaca atataaagat gatagtcaga taagaaattc
    13261 gacaagtgcg agtaaagtga tggtaatgaa tcaagggctt gaagtgattg atttggcgat
    13321 gagaatagtt ggtgctaaaa gcttagaaat gaatcgacca ttacaacgtt actatcgtga
    13381 catgcgtgca ggattacaca atccacctat ggaagatgca gcatatacaa atattgctaa
    13441 gagtattaca gatacatttt aaatttaaaa aggtaaatcg atcgttgatt ggcataaaga
    13501 gatgaaattt gacgttcgga tttacctttt ttgtgggaat ttataggtat attaagatat
    13561 tttgctaaat tttttgcgaa aataaaaaaa taaataacac tagctattgt aaatgtatat
    13621 gcaatcttgt ataattttgg aatgattgat aatcattttc aataggagga aattatgaaa
    13681 aaactattat taccattaat aattatgtta ttagtgttag ctgcgtgtgg gaaccaaggt
    13741 gaaaaaaata acaaagctga aactaaatct tataaaatgg acgatggcaa aacggtagat
    13801 attccgaaag accctaaacg cattgcagta gttgcgccaa catatgctgg tggacttaaa
    13861 aaattaggtg caaacattgt agctgtaaat caacaagttg atcaaagcaa agtgttaaaa
    13921 gataaattta aaggtgttac aaaaattggt gatggcgatg tagaaaaagt tgctaaagaa
    13981 aagccagatt taattattgt atactctact gacaaagaca ttaaaaagta tcaaaaagta
    14041 gcaccaacag tagttgttga ctataataag cataaatatt tagaacaaca agaaatgtta
    14101 gggaaaattg ttggtaaaga agataaagta aaagcttgga agaaagattg ggaagaaaca
    14161 actgctaaag acggtaaaga aattaaaaaa gcaattggac aagatgcaac agtgtcattg
    14221 tttgatgaat ttgataaaaa attatacact tacggcgata actggggtcg tggtggagaa
    14281 gtattatatc aagcatttgg tttaaaaatg caaccagaac aacaaaagtt aactgcaaaa
    14341 gcaggttggg ctgaagtgaa acaagaagaa attgaaaaat atgctggtga ttacattgtg
    14401 agtacaagtg aaggtaaacc tacaccagga tatgaatcaa caaacatgtg gaagaatttg
    14461 aaagctacta aagaaggaca tatagttaaa gttgatgctg gtacatactg gtacaacgat
    14521 ccttatacat tagatttcat gcgtaaagat ttaaaagaaa aattaattaa agctgcaaaa
    14581 taattcagct atataagcca gtgaaatgag agtctgaaac atatcaatct tttgatattg
    14641 tattaggctc ttatttttat agcaagaagg ttagattttt gtattttttt aaataataag
    14701 tgccgttgtt atcattcaat ttaattaatg atagattagt attatttcag ctaaagtagt
    14761 atgcctgaga aaatagctca atgtatctct gcattaataa gttatatcat aattatttta
    14821 gtgcatactt tatggtaggg atagcaggga atggctatca attaaagaag aggtttaaaa
    14881 ggattacaac agaatgttat gattttgtag aaagatatat aacaacgttt tataaaaaca
    14941 taatattgtt aatggaaaat gaaatgtaag ggggatttcg agtgactaag aaagtttatt
    15001 ttaaccatga tggtggtgta gatgatttag tatctctatt tttattatta caaatggaaa
    15061 acgttcaatt gataggggtc agtacaattg gtgctgattg ttatttagag ccatctttga
    15121 gcgcatcagt aaaaattatt aatcgttttt caaatgaaga tattcaagtt gcgccatcat
    15181 atgaacgagg aaaaaatcca tttcctaaag aatggcgtat gcatgccttt tttatggacg
    15241 cattgccaat tttaaatgag ccagtcaaac acgttgcttc aaatgtgagc gacaaagaag
    15301 cctttgaaga cattattcaa actttaaaga gacaatcaga aaaagtaaca ttattattta
    15361 caggcccgct tacagattta gcaaaagcac tacaaaaaga tacatctatc gttcagtata
    15421 tagaaaaatt agtttggatg ggtggtacct ttttaccaaa aggaaatgtt gaagaacctg
    15481 agcatgatgg ttctgcagaa tggaatgcat attgggatcc agaagcggtt aaaattgttt
    15541 ttgatagcga tatagagatt gatatggttg ctttagaaag tacgaatcaa gtaccgctaa
    15601 cgttagatgt tagacaaaga tgggcgaatg aacgccaata tacgggcatt gattttttag
    15661 gagtaagtta tgcagcggta ccaccattaa cacactttat aacaaattct acttactttt
    15721 tatgggatgt tttaacgact gcttatattg gtaacaagga cttagttcat tcaattgaga
    15781 aaaaagtgga tgtaataagt tatggaccaa gtcaaggtaa gacatttgag tgtaaagatg
    15841 ggcgcaaaat taatgtcata aatcatgtag ataacaacgc attttttgat tatataactg
    15901 cacttgctaa aaaagtaaat taacagctgt gtagaataat taaggtttta atttatatag
    15961 aacaacttat tgtaaacttt tcgttcctta aagtttacaa tggtgctata ataatggtca
    16021 tgaaatacga aaggaagtaa aaaatgacaa caaaacagtt agtatataca gctttaatga
    16081 cagcgattat cgctatttta ggattggtac cggtaattcc actaccattt tcttcagtac
    16141 caattgtact tcaaaacatt ggtattttct tagcaggtgt gattttagga cgtaaatatg
    16201 gcacattaag tgttatcgtc tttttattat tagtagttgc tggtttgcca ctgttatcag
    16261 gtggtcgcgg tggcattggt gtattcgcag gtccttcagc agggttttta ctattatatc
    16321 cagttgtagc attcatgatt ggtgcgattc gtgatagatt catcaatgaa attaatttct
    16381 ggattttatt cgttggtatt ttagtttttg gtgttatagc attagatgtt attggtacat
    16441 tgattatggg catgattatt aacataccat ttacgaaagc tatttcaatt tcattagctt
    16501 atttgcctgg tgatatatta aaagcaattg tagcaagttt gattggtaca gctttactta
    16561 atcactcgca gtttcgtcaa attatgggat taaaataatc atatttaaga tagtaaagta
    16621 attgaataag ttgctttgaa atttataaaa gtgaaaggag taggtgtcaa tggctagtat
    16681 aagtatgtca gatattaatt gcgacggtac aatatttgaa aataacgaca agcagttgat
    16741 ttatttaacg cctatttttc cacaacgata tacaagcaat acatggttat ataaaaagat
    16801 gcctacccaa gagcgatggc tgaaagactt agaacgtcaa catcaattac atacaaatca
    16861 aggttcaaat cattatgcgt ttagtttccc ggaaaatgaa caacttgata atcattggat
    16921 ggctatgttt aaagatatga attttgaact aggcattatg gaattgtatg ccatagaaag
    16981 tgatgcgctt gccaatttgc cacgtaactc tgacgttgaa attgccatcg ttgacgagtc
    17041 gcatatagat gcctatttaa aagttgcata tcagtttagt ttgccatttg gaaaagacta
    17101 tgcagatgca catgaaaaaa tggtaaggga acattatcaa aaagatgtga ttaaacgctt
    17161 agtagcttat ttaaataatg aacctattgg cgttgtagat gtcattgaaa gtgaaaatta
    17221 cattgaatta gatggatttg gtgtactaga acaatttcga caccaaggaa tcggttctac
    17281 catgcagtca ttagtaggtg aatatgcttt atctagaaat cataaaccgg ttatattggt
    17341 ggcagatggt gaagatacag caaaagatat gtatgtaaaa caaggctatg tctatcaatc
    17401 gttttgttat caaatattaa aagaaaacat tgaaatttaa cgattgatga taggtttgta
    17461 ttagaataaa tgaaaaatat aaaaagcagt aagctggttt cgagtagaaa tcgacttact
    17521 gctttttaag ttgtgataag tatattaaac aatgttgttt taaggttatt aatccatgta
    17581 agcattgatc attatgacac actataatga atatgttatt gttcagaatc aatgatacgt
    17641 tctggatgac tgtatatatt aaagccacca tttcgaataa atccaactgc cgtaatattt
    17701 aggtcattag ctaaggttac agcaagcgtt gtcggagcag atttagataa aatgacgcca
    17761 acaccaattt tcgcggcttt aattaaaatt tctgatgaaa tacgtccact gaaaattaat
    17821 actttatctc ggacagtaat atgtcgctga atacaaaatc catataattt atctagagcg
    17881 ttatgtctgc caatgtcttg tcgatgtaca aaaaatgtca aaccatcact tatagcagca
    17941 ttatgcaggc cacctgtttc ttggtaaata tgactggcac tttgtaaacg cgtcatcata
    18001 ttaataattt gttgtggtgt taaagtgatt ttagacatag atgttttagc aacagcagca
    18061 tcattttgaa aataaaactc acgactcttt ccacaacaag atgcaatcat tcgttttgtg
    18121 gaatattgaa agcgatcgcc taaatcttta ttcagttcaa catgggcaaa tcctttacta
    18181 tcatcaatca gtacagattt taattcatct cgctttaaaa tggcaccttc cgaagccaga
    18241 aatccaatga ctaactcctc aaggtttgtt ggactgcata taacagtcgc aaattcttca
    18301 ccattcacca taattgtaag tggaaattct gtcacatatt gatctgttgt attgaataat
    18361 tttccatctt catatctaac aattggttga cctaaagata catctttgtt cattatctaa
    18421 cccctttaat tagcttgaac tttattttaa atcaatatgc ttaatatttt aacatatttg
    18481 cttaaaactg aaatttgatt aactaaataa aatagcaggc aattatttag aatttgattg
    18541 gatttagaaa aaggcacact agcagctcaa ttaataaaaa ataattttta gaaagtgtga
    18601 caattatttg aatcattatt taaagagaaa acgcttcttt tcaaaaatat tgttaagtgt
    18661 ataatgagag tgtaaatatt taataattgg gggtatttca taatgaaaat gaaacgtttt
    18721 atagctattg taatggcatt atttttagta ttagctggtt gttctaattc taatgataat
    18781 caagacaaca aaaaagatgc agcagacaat ggaaaaaaac aagaaattca agttgcagcg
    18841 gcagcaagtt taacagatgt tacaaaaaaa ttagcatcag aatttaaaaa agaacataaa
    18901 aatgctgata ttaaatttaa ctatggtgga tcaggagcat taagaaaaca aattgaatca
    18961 ggcgcacctg ttgacgtatt tatgtctgca aatactaaag atgtagatgc attaaaagac
    19021 aagaataaag cacatgatac atataaatat gcgaaaaata gtctagtatt aattggtgac
    19081 aaagattcaa attacacttc agtaaaagac ttaaaaggca atgataaatt agcattaggt
    19141 gaagtgaaaa ctgtgccagc gggaaaatat gcgaaacagt atttagataa caataacttg
    19201 tttaaagaag ttgaaagtaa aatcgtttat gctaaagatg taaaacaagt attaaattat
    19261 gttgaaaaag gtaatgcgaa acaaggtttt gtatataaaa ctgacttata taaacaaaat
    19321 aaaaaaattg atactgtaaa agtaattaaa gaagtagaac ttaagaaacc aatcacatat
    19381 gaagctggtg cgacatcaga tagtaaatta gctaaagagt ggatggaatt cttaaaatca
    19441 gataaagcta aagaaatatt aaaagaatat cactttacag cataaggagt tgtaatccat
    19501 gcctgactta acaccttttt ggatatcaat acgagttgct gtaatcagta cgattattgt
    19561 aacggtttta ggtgttttta tatctaaatg gttgtatcgt cgtaaaggtt cgtgggttaa
    19621 agtattggaa agtttattga tattacctat tgttttgccg ccaacggtat taggttttat
    19681 tctattaatc atcttctcgc caagaggacc aatcggtcaa ttctttgcga atgtactaca
    19741 tttacctgta gtgttcactt tgacaggtgc tgtgatagca tctgtcattg ttagttttcc
    19801 actaatgtat caacatactg tgcaaggctt cagaggtata gacacgaaaa tgattaatac
    19861 agctagaacg atgggagcaa gtgaaacgaa aattttcctc aaattaattt taccattagc
    19921 taaacgctct attttagcag gtataatgat gagttttgct cgtgcattag gtgagtttgg
    19981 tgctacatta atggttgcag gatatattcc aaataaaacg aatacactac ctttagaaat
    20041 atacttctta gtggaacaag gtagagaaaa tgaagcgtgg ttatgggtat tagtgctagt
    20101 cgcattctct attgtggtta tatctacaat taatttattg aataaagata aatataagga
    20161 ggtcgactag atgcttaaaa tcaatttaaa atatcaatta aagaacactt taattcgcat
    20221 caatatagat gatactgaac caaaaattta tgcagttcgt ggtccatctg gcattggtaa
    20281 aactactgtt ttaaatatga ttgccggatt acgtaaagca gatgaagcta ttatcgaagt
    20341 gaatgggcaa ttgcttactg atacggcaaa aaacgtgaat gttaaaattc aacaacgacg
    20401 tattggatat ttatttcaag actaccaact gtttcctaat atgacggtct ataaaaatat
    20461 tacttttatg gctgaaccat ctgaacacat cgatcaatta atccaaactt taaatattga
    20521 tcatttgatg aaacaatatc ctatgacatt gtcaggtgga gaggcacaac gtgtagcact
    20581 tgcacgtgca cttagcacga aaccagattt aattttatta gatgaacctt tttctagttt
    20641 ggatgatact acaaaagatg agagtattac attagttaaa cgtattttca acgaatggca
    20701 aataccaatc atatttgtga cacattcaaa ctatgaagca gaacaaatgg ctcatgaaat
    20761 tattacaatt ggttaatgat acatttgcct tgaaagagtt tagaacgtat ttaaaaatgt
    20821 agaagtgcaa gcttctatta gcattttaat gatgttttaa actctttttt aggcgcaatt
    20881 tttgagagac attgacgcac gtcatataat gaaagtaagg ataaatagaa aggataactt
    20941 aatgtgagtc aagaacgtta ttcaaggcaa attttattta aacaaatagg tgaaataggt
    21001 caaaacaaaa taaatcaaaa atgcgcgttg attattggta tgggcgcatt gggtacacat
    21061 gtggctgaag gtcttgtgag agcaggcatt gctaaactaa tcattgttga cagagattat
    21121 attgaattta gtaatttaca aagacaaaca ttgtatactg aagaagatgc tttgaaaatg
    21181 atgcctaaag tggttgcagc taaaaagcat ttgctagcgt tacgtagtga tgttgatatt
    21241 gatgattata ttgcccatgt ggattattat tttttggaaa cacatggaca ggacgttgac
    21301 gttatcattg atgcaaccga taactttgaa acacgacaac tgattaatga ttttgcatat
    21361 aaacatcgta taccttggat ttatggaggt gttgtacaga gtacatatac agaagctgca
    21421 tttatacctg gtaaaacacc ttgctttaac tgtttggtac cacaattgcc agcattaaat
    21481 ttaacatgtg atacagtagg agtcattcaa cctgccgtga cgatggcaac aagtttacaa
    21541 ttaagagatg cgatgaaagt attaacggaa caaccaattg acacaaaaat aacttatggc
    21601 gatatttggg aaggtagtca ttattcattt ggtttcagta aaatgcaacg ttcagactgt
    21661 acaacttgtg gagatgtacc aagttatccg tatttaaaca agaatgaaca acgttatgca
    21721 acattgtgtg ggagagacac tgtacagtat gaaaatacat caattacaca cgatattctt
    21781 gttcaatttt taaaacaaca tcaattaaat tatcgcagta attcgtatat ggttatgttt
    21841 gaatttaaag gacaccgcat tgttgctttt aaaggtggaa ggtttttaat acatggcatg
    21901 acacgcacat cagatgccac acatctaatg aatttattgt ttggataaaa aagataagac
    21961 aaaaggagtg taatattatg ggcgaacatc aaaacgttaa attgaatcgt acagttaaag
    22021 cagccgtact aacggtatca gatactagaa actttgatac agataaaggt ggtcaatgcg
    22081 tgcgccaatt gttacaagca gatgacgttg aagtgagtga cgcacattat acaattgtga
    22141 aagatgaaaa agtagccatc acgacgcagg tgaagaagtg gttagaagaa gatattgatg
    22201 tcatcattac gactggtgga acgggtattg cacaacgtga tgtgacgatt gaagcagtaa
    22261 aaccactttt aactaaagag atagaaggct ttggggaatt atttagatat ttgagttatg
    22321 ttgaagatgt tggcacgcgt gcattattgt ctcgtgctgt agcaggtaca gttaataata
    22381 aattgatatt ttcgattcca ggatcaacag gcgcagttaa attagcatta gaaaagctca
    22441 ttaaaccaga attaaatcat ctgattcatg agcttacaaa ataatttatt gatctaattg
    22501 gcgttgaaaa tctccagatt taccgccaga cttgctttca aggtaggttt cgccaataat
    22561 cataccttta tcaactgctt tcgtcatgtc gtaaatggtt aaagccgtta ctgatgcagc
    22621 ggttaaagct tccatttcaa caccggtttt gccagttgta gagacagttg tttgaatgtt
    22681 taaagtataa aggggtgcat ttgtttcatc ccagttgaag tgaacatcta tgccagtcaa
    22741 tggtaatgga tggcacatcg gaataattgt tgatgtattt ttggcagcca taataccagc
    22801 gatttgagca gtgttcaata cattgccttt ggtattagtg ttattagaaa tctgcttata
    22861 aatagtttca tttacagtaa tgcttgaatg agcgacagca gttctttttg taatttgttt
    22921 gtctgataca tcgaccattt tggcgtggcc ttgttgatta atatgagtaa actcagtcat
    22981 tttacccctc ctagtgcatc tagtatatca tgaaaaaata aaagttttgg agatgatttt
    23041 taatggtagt agaaaaaaga aacccaatcc cagttaaaga agcaattcaa cgtatcgtta
    23101 atcagcagag tacaatgccg gcaattacgg tagcacttga aaaaagtcta aatcatatct
    23161 tagcagaaga tattgtagct acttatgata taccaaggtt tgataaatca ccttatgatg
    23221 gttttgcaat tcgcagtgtt gattcacaag gggcaagtgg tcagaatcgc attgagttta
    23281 aagtgattga tcatattggt gcaggttcag tttctgataa attagttggg gatcacgaag
    23341 cggtgcgtat tatgactgga gcacaaatac ctaatggcgc agatgctgtt gttatgtttg
    23401 aacaaacgat tgaactagaa gatacattta caattcgtaa accattttca aaaaatgaaa
    23461 atatatcttt aaaaggtgaa gaaacaacga caggcgatgt tgttctaaaa aaaggacaag
    23521 taattaatcc aggggctatt gcggtccttg caacatatgg ctatgcagag gttaaagtta
    23581 ttaagcaacc gagcgtcgct gttattgcaa caggaagcga attattagat gttaatgatg
    23641 tattagaaga tggaaaaatt cgtaactcta atggtccaat gattcgtgcc ttagcagaaa
    23701 aattaggtct tgaagttggt atttacaaaa cacaacaaga tgatttagat agtggtatac
    23761 aagtcgttaa agaagctatg gaaaaacacg atatcgttat tacaacgggc ggcgtttctg
    23821 taggagactt tgactattta cctgaaattt ataaggctgt aaaggcggaa gtgttattta
    23881 ataaagtagc aatgcgtcct ggcagcgtaa caacggttgc atttgcagat ggaaagtatt
    23941 tgtttggatt atctggaaat ccatcagctt gttttacagg atttgaacta tttgtgaaac
    24001 cagctgttaa acatatgtgt ggcgcactag aagtcttccc gcaaataatt aaagcaacat
    24061 taatggaaga ttttaccaag gcaaatccat tcacacgatt tatacgtgct aaagcaacgt
    24121 taacaagtgc tggtgctact gtagtacctt caggattcaa taaatcaggt gcggttgtag
    24181 cgattgcaca tgctaactgt atggtcatgt taccaggagg gtcacgtggt tttaaagcgg
    24241 gtcatacagt agatattata ttgactgaat ctgacgctgc tgaagaggaa cttcttttat
    24301 gattttacaa attgtagggt acaaaaagtc tggtaagaca acattgatga ggcatattgt
    24361 ctctttctta aaggcacatg gttatacagt agctactatt aaacatcatg ggcatggtaa
    24421 ggaagatatt caattacagg attcagacgt cgatcacatg aagcattttg aagcgggggc
    24481 agatcaaagt attgtacaag gttttcaata tcagcaaact gtaacacgtg tagataatca
    24541 aaatcttact caaattattg aaaaatctgt tacaattgac accaatatcg tattagttga
    24601 aggctttaaa aatgctgatt ttgaaaaagt cgtagtctat cgagatgaag aagagttaca
    24661 agtattacaa caattgtcga atgtttgtta tagcattaat gttagggagc atgaagattt
    24721 tacagcattt gagcaatggt tattaaataa aattaaaaat gattgtgata cacaattaac
    24781 atagaggatt gaaatgaatg aaacaatttg aaatcgtgac agaaccgata caaacagaac
    24841 aatatcgtga attcactata aatgaatatc aaggtgcagt agttgttttt accggtcatg
    24901 ttcgcgaatg gactaaaggc gtcaaaacgg aatatttaga atatgaagcg tatattccaa
    24961 tggctgaaaa gaaattggca caaattggag atgaaataaa tgaaaaatgg cctggaacga
    25021 taacgagtat tgttcataga atagggccat tacaaatttc agatatagct gtattaattg
    25081 cagtttcttc accgcatcgt aaagatgcct atcgagcaaa tgaatatgca attgagcgta
    25141 tcaaagaaat tgttccgatt tggaaaaaag aaatttggga agatggttca aaatggcaag
    25201 ggcatcaaaa agggaattat gaagaagcga agagggagga ataagagaga tgaaggtact
    25261 ttacttcgca gaaattaaag atatattaca aaaagcacag gaagatattg tgcttgaaca
    25321 agcattgact gtacaacaat ttgaaatttt attgtttgaa cgttatccgc aaatcaataa
    25381 taaaaagttt caagttgctg taaatgagga atttgtacaa aaatccgatt tcattcaacc
    25441 taatgatacc gttgcattaa ttccaccggt tagtggaggt taagcgagca tgaaagcaat
    25501 aattcttgca ggtggtcatt cagtaagatt tggtaagccc aaagcttttg cggaagtgaa
    25561 cggtgagacc ttttatagta gagtaattaa gacattagaa tcaacaaata tgttcaatga
    25621 aattattatt agtactaatg cgcaattggc aacgcaattt aaatatccaa atgttgttat
    25681 agatgatgag aatcataatg ataaaggtcc attagcagga atttatacaa tcatgaagca
    25741 acatcctgaa gaagaattgt tttttgtcgt ttctgttgat acaccaatga ttactggtaa
    25801 agctataagc acgttgtatc agtttttagt ttctcatctt attgaaaatc atttagatgt
    25861 cgcagctttt aaagaagatg gacgttttat tccaacaatt gcattttata gtccgaatgc
    25921 attaggcgct ataactaaag cactacattc tgataattac agttttaaaa atgtatatca
    25981 tgaattatca acggattctt tggatgtcag ggatgtagat gcgccctcat attggtacaa
    26041 aaatataaat tatcagcatg atttggacgc tttaattcaa aaattgtaag ccgttaggag
    26101 gtccacaaat ggtagaacaa ataaaagata aactaggacg tcccatccgt gacttacggt
    26161 tatctgtgac agatcggtgt aactttaggt gtgattattg catgcctaaa gaggtatttg
    26221 gagatgattt cgtattttta cctaaaaatg aactgttaac gtttgatgaa atggctagaa
    26281 tcgctaaggt atatgcagaa ttaggtgtaa aaaaaatacg cattacaggt ggagaaccat
    26341 tgatgcgacg ggatttagat gtacttattg ctaaattaaa tcaaatcgat ggtattgaag
    26401 atattggttt gactacaaat ggtttgttat taaaaaagca tggacaaaag ttatatgatg
    26461 ctgggctacg cagaattaat gtcagtttgg atgctattga tgatacgcta tttcaatcaa
    26521 tcaataatcg taatattaaa gcgactacga ttttagaaca aattgattac gcgatgtcta
    26581 ttggtttgaa tgtaaaagta aatgttgtta tacaaaaagg tattaacgat gatcaaatta
    26641 taccaatgct tgaatatttt aaagataaac atatagagat tcgatttata gaatttatgg
    26701 atgttggtaa tgataatgga tgggatttca gcaaagttgt aactaaagat gaaatgctta
    26761 ctatgataga gcagcacttt gaaattgatc ctgtagaacc aaaatatttt ggggaagtag
    26821 caaaatatta tcgacataag gataatggtg ttcaatttgg tttgattaca agtgtttcac
    26881 aatcattttg ttctacatgt acacgggcaa ggctgtcatc agatgggaag ttttacggat
    26941 gtttatttgc aactgccgat ggatttaacg ttaaagcgtt tattcgttct ggcgtgaccg
    27001 acgaagaatt aaaagagcaa tttaaagctt tatggcaaat aagagatgat cgatattcag
    27061 atgagagaac tgctcaaaca gttgccaatc gtcaacgtaa aaagataaac atgaattata
    27121 ttggtggtta atgtgtagga accactacat attaaatcat tagagattct ttaatatttc
    27181 tgtcttactc cctaaaatac aatattattt attaaagtaa aaacggtcat atctatgcca
    27241 aatttaatag aaatgatcgt ttttaatgtt tttacaaatt ggcgaggccc caacacagaa
    27301 gttgacgaaa agtcagctta caataatgtg caagttgggg aacgggaccc caacatagag
    27361 aatttcaaaa agaaattcta cagacaatgc aagttggcgg ggccccaaca tagagaattt
    27421 caaaaagaaa ttctacagac aatgcaagtt ggcggggccc caacatagag aatttcaaaa
    27481 agaaattcta cagacaatgc aagttgggga acaacgaaat acattttatg aaaatatcat
    27541 ttctatccca ctcttaagaa tcactacata ataaatcttt agtggttctt taacattgtt
    27601 atcacattcc aagtcattga gttgtaatat atctttttta ggtataaatg ttgtcgaata
    27661 aacaacaagt tgtccaaaag atataaatct aaacaagata tagccatcaa tttaatattt
    27721 gtaatagata aaatgctaag tttgatatat aataaaatta agtaattgta taataatatg
    27781 aattacaaac atctaagaag aaacatagga ggcatcatat tatgagtaat aaagttcaac
    27841 gttttataga agcagaaagg gagttaagtc agttaaagca ctggttaaaa acaacacata
    27901 agatttcaat tgaagaattt gtagtacttt ttaaagtgta tgaagctgaa aagattagcg
    27961 gtaaagaatt gagggataca ttacattttg aaatgctatg ggatacaagt aaaatcgatg
    28021 tgattatccg taaaatctat aaaaaagagc ttatttctaa attgcgttct gaaacggatg
    28081 aaagacaagt attctatttc tatagtactt ctcaaaagaa attgttagat aaaattacta
    28141 aagaaataga agtgttaagc gttacaaact aaaaacttaa aaagcatgcc aatcagtaca
    28201 tcataattgc gtcttgggga cagacaaatg atgaatagag attggcatgc tttttatttt
    28261 tgaatataaa tatttagttc atggcatttc tagctacatg acgtccatga attaagaagt
    28321 aaacaagcat agtaatgatt gctaaagcgg ccataaagcc gaagatttca ctatatgaaa
    28381 acatatgagt aaatagccca aggaatgatg gaccgaagcc gacaccagca tctagaccaa
    28441 cgtaaaaagt agatgtcgcg ataccatatt taatcggggg tgagactttt atcgcaatag
    28501 attgcattgc agatgataaa tttccatacc ctaaacctag gcaagcacca gcaagtaata
    28561 ttaaccagct ttgatagctt gaaattaagc acacaaatga aagaaaaagc atgataaatg
    28621 ctgggtagac aataatattt tcatttttat catccatcaa tctaccagca ataggtctag
    28681 taattaacga tgctatagca tagcaaataa agaaatagct tgctgcagtt actaggtgtc
    28741 gctctaaagc aaatgcttgt aaataagtta ggatggacgc ataggtaacg ccaattaaaa
    28801 gcataataac agcaacaaga atggcctctt ttgcaataaa ttgatgaata ctaaatcttg
    28861 gtttatcaat gacattagtt tcagttttgc tatttgttac ttcgaaatca acttttataa
    28921 ataatgagat aatgagtccg agtatgccta atatgacaca aataacaaac agtaaatcaa
    28981 ttgcgtattt tgtaataagt aacataccta gaaatgggcc aatcgctgta cctaatacta
    29041 aacttaagga aaataaactg atgccttcac tttttctatt aacaggggta acgtatgccg
    29101 caatagtacc tgttgcagtt gtcacaactg cagttgcgat accgtttatg agacgtacaa
    29161 agattaaaaa agctaaagat ccatcaataa aataaagtaa ttgcgtgata attaaagcaa
    29221 ttaaaccaat aaataataat cgtttaggtc cgatttgatt tacaaattta cctgtagcaa
    29281 atcgaccaat taaagacccg acaataaata gtcctacaac taatccggct aatgaatcgg
    29341 aagcgtcgaa agcttgctta ctatagccag caataactac aagtaacaaa tacatgcata
    29401 ggtatacgat aaagttgaca aaaaagttaa ttgtaaaact tttggtgaaa ataggtgatt
    29461 tatttaatga gccttgatta gtcattgtgt gcctccttta ataatatttc atgaatttga
    29521 ttcatcacat ttatggtttc ttgcatttga tcttgcgaaa tgtttgtttt ggcaactact
    29581 gcttgttgga gcttttgtac atcaagacaa acaatctcat ataattcatg ccctttaggt
    29641 gttaaagtta aaaacttttg tcgtttatcc tctaaactgt tttctactgt tataaggtca
    29701 ttttctatta gagcttttaa agtttttctt gcagtaggct tttcaattgc acgtctatgt
    29761 gaaatttcaa tgagagtagt gggctgatgt ttagcgatat cgcgcaagat taaccattgg
    29821 ccataatata tattgtgttt ttctaaaatc ggctcggcta attttaaata cgggcgatat
    29881 attgttataa aactattgaa aaattcttgt gatagcatag tgacatctcc taagacaaaa
    29941 tagttagctt agctaacctt tttacaacaa tagtaattat aaaacgggag caattagaaa
    30001 tcaatatata attattaaga gcaaaaatat ttatactttg ttaaaataag cgtaattaca
    30061 tgtaaatagg gggatactaa tgatattgaa atttgatcac atcattcatt atatagatca
    30121 gttagatcgg tttagttttc caggagatgt tataaaatta cattcaggtg ggtatcatca
    30181 taaatatgga acattcaata aattaggtta catcaatgaa aattatattg agctactgga
    30241 tgtagaaaat aatgaaaagt tgaaaaagat ggcaaaaacg atagaaggtg gagtcgcttt
    30301 tgctactcaa attgttcaag agaagtatga gcaaggcttt aaaaatattt gtttgcgtac
    30361 aaatgatata gaggcagtta aaaataaact acaaagtgag caggttgaag tagtaggtcc
    30421 gattcaaatg gaaagagata cacataaaga tggtaagata aagtggcaat tgctttatat
    30481 aatgaatcag gatgatgatg aaattaagcc accatttttt attcaatggg aagaaagtga
    30541 ttccatgcgt actaaaaaat tacaaaaata ttttcaaaaa caattttcaa ttgaaactgt
    30601 tattgtgaaa agtaaaaacc gatcacaaac agtatcgaat tggttgaaat ggtttgatat
    30661 ggacattgta gaagagaacg accattacac agatttgatt ttaaaaaatg atgatattta
    30721 ttttagaatt gaagatggta aagtttcaaa atatcattcg gttatcataa aagacgcaca
    30781 agcaacttca ccatattcaa tttttatcag aggtgctatt tatcgctttg aaccattagt
    30841 ataaatatac gtaagtgcta tgagcgagaa tgcccatatg aataacggca agcgcaatgg
    30901 aaagaatcgt taatatatta tttaatcgtg atgacttaat taaaatgaaa aagattgata
    30961 atataaatgt gaaaaagata agtataaccc gtaaactaaa gtaattcacg gtgagaggtt
    31021 gactcaatgt cataatgatt gcaacgatgt tcataattat aaatagactt aaaataattg
    31081 ttctcatatc aaacacctca ttgttagatt attgacatta taacaggggt aattgtatat
    31141 gaacattaat gtggttgctt gaggagaaat ttattcattg aagtcaaatt ggttcatgtt
    31201 agaaatgaat atcgtgttag atgatgaaag tatattgaag tataggtaac tagttgaaaa
    31261 gtattaattg tacgataaca ttaaatttaa caggaagcat agatataaaa tgattcacaa
    31321 ttaaaatgag taaatttgaa cttgctaaac tattgattgg agcatggaca tttcaaaaat
    31381 aagagttcaa atcatacaca agctctgaat cgacactata agatacaaac tgtataatta
    31441 aaggtattgt taaatagaag gagatatcat aaatcatgga aaagatgcat atcactaatc
    31501 aggaacatga cgcatttgtt aaatctcacc caaatggaga tttattacaa ttaacgaaat
    31561 gggcagaaac aaagaaatta actggatggt acgcgcgaag aatcgctgta ggtcgtgacg
    31621 gtgaaattca gggtgttgcg cagttacttt ttaaaaaagt acctaaatta ccttatacgc
    31681 tatgttatat ttcacgtggt tttgttgttg attatagtaa taaagaggcg ttaaatgcat
    31741 tgttagacag tgcaaaagaa attgctaaag ctgagaaagc gtatgcaatt aaaatcgatc
    31801 ctgatgttga agttgataaa ggtacagatg ctttgcaaaa tttgaaagcg cttggtttta
    31861 aacataaagg atttaaagaa ggtttatcaa aagactacat ccaaccacgt atgactatga
    31921 ttacaccgat tgataaaacg gatgatgaat tattaaatag cttcgaacgc cgaaatcgtt
    31981 caaaagtgcg cttggcatta aagcgaggta cgacagtaga acgatctgat agagaaggtt
    32041 taaaaacatt tgctgaatta atgaaaatca ctggggaacg cgatggcttc ttaacgcgtg
    32101 atattagtta ctttgaaaat atttatgatg cgttgcatga agatggagat gctgaactat
    32161 ttttagtaaa gttggatcca aaagaaaata tagcgaaagt aaatcaagaa ttgaatgaac
    32221 ttcatgccga aattgctaaa tggcagcaga agatggaaac atctgaaaag caagctaaaa
    32281 aagcgcaaaa tatgattaat gatgcgcaaa ataaaattgc taaaaatgaa gatttaaaac
    32341 gagacctaga agctttagaa aaggaacatc ctgaaggtat ttatctttct ggtgcactat
    32401 taatgtttgc tggctcaaaa tcatattact tatatggtgc gtcttctaat gaatttagag
    32461 attttttacc aaatcatcat atgcagtata cgatgatgaa gtatgcacgt gaacatggtg
    32521 caacaactta cgatttcggt ggtacagata atgatccaga taaagactct gaacattatg
    32581 gattatgggc atttaaaaaa gtgtggggaa catacttaag tgaaaagatt ggtgaatttg
    32641 attatgtatt gaatcagcca ttgtaccaat taattgagca agttaaaccg cgtttaacaa
    32701 aagctaaaat taaaatatct cgtaaattaa aacgaaaata gattaacgac tgaaatctga
    32761 acgctcataa gactgtcatt tgcgttcaga tttttttaca caatatagaa tggttgagta
    32821 aaatattttt gaatatagtg aaagaggggg aagtactgtg ataaaaaagc tattacaatt
    32881 ttctttgggg aataagtttg ctatcttttt aatggttgtt ttagttgtct tgggcggtgt
    32941 atacgcgagt gccaaattga aattagaatt actaccaaat gtacaaaatc cagttatttc
    33001 agttacaaca acaatgccgg gtgcaacgcc acaaagtacc caagatgaaa taagtagtaa
    33061 aattgacaat caagtaagat cgttggcata tgtgaaaaat gttaaaacgc aatccataca
    33121 aaatgcttca attgtaacag ttgaatatga aaataataca gatatggata aagcagaaga
    33181 acagcttaaa aaagaaatcg ataaaattaa atttaaagat gaagttggtc aaccagaatt
    33241 aagacgtaat tcgatggacg cttttccggt tttagcatat tcattttcaa ataaagagaa
    33301 tgacttgaaa aaagtaacga aagtactgaa tgaacaatta ataccaaaat tacaaacggt
    33361 agatggtgtg caaaatgcgc aattaaatgg gcagacgaac cgtgaaatca cccttaaatt
    33421 taagcaaaat gaacttgaaa aatatggatt gactgctgat gatgtagaaa actatctaaa
    33481 aacggcaaca agaacaacac cacttggatt gttccaattt ggtaataaag ataaatcaat
    33541 tgttgttgat ggtcagtatc aatctgttga tgcttttaaa aacataaata ttccattaac
    33601 attggcagga ggacaagggc aatctcaatc ccaaagtgac aataaacaaa attcagccat
    33661 gtcagacgtt aatcaggcat caccacagca aaattcaaaa gcgtcagcat caaataatgt
    33721 aagtggtatg ccgacagcga aactaggaga tttagctgat attacagttg gtgatgtgcg
    33781 tacttctatt tctaaaacga atggcaagga tgcggttaat ctacaaataa ctaaagctca
    33841 agatgccaat acagttcaag tagccaaaga tgtacagcgt aaaattgata cattcgttga
    33901 tgaaaataaa gattttaatg tcacaaaaac aatggatact gcaaagcctg ttgagaaatc
    33961 actttatacg atggttgaaa aagcatcatt aggtacaatc gtggcaatta tagttatttt
    34021 gctgttttta agaaacattc gtacgacggc aatttctatt atatccattc cgttatcact
    34081 tcttatggcg cttattgctc tgaaattgag tgatgtttca ttgaatatac taacgttagg
    34141 tgcattaaca gtagcgattg gacgtgtgat agacgattcg attgtagttg ttgaaaatat
    34201 ttatcgacgc ttaacagatt cagaagaaca actaaaaggt gaaaatttaa ttgtcagtgc
    34261 gacaactgaa gtatttaaac caataatgtc atcgacacta gttactatta tcgtcttctt
    34321 accacttgtg tttgtatcag gttcagtagg cgaaatgttt agaccttttg cattggctat
    34381 tgcatttagt ttattagcat cgttattagt gtcaattaca ctcgttccag cgttggcagc
    34441 tacactattt aaaaaaggcg ttaaacgtcg taataaacaa catcaagaag gattaggtgt
    34501 tgttagtaca acttataaaa aagtattgca ttggtcacta aatcataagt ggattgtaat
    34561 tatattaagt acattaattt tggttgtaac tattgtattt ggaggaccga gactaggcac
    34621 tagctttatt tcagcaggtg acgataaatt tttagctatt acttatacac cgaagcctgg
    34681 tgaaacggag caagcagtgt tgaatcatgc gaaagatgtt gaaaaatatt taaaacagaa
    34741 aaagcatgta aaaacaattc aatactcagt tggcggtagt agtccagtag atccaacggg
    34801 tagtacaaat agtatggcaa tcatggttga atatgataat gacacgccta attttgatgt
    34861 agaagcggat aaggttatta aacatgcaga tggctttaaa catcctggag agtggaaaaa
    34921 tcaagattta ggaacaggtg caggtaataa atctgtagag gttactgtga aaggtccatc
    34981 tatggatgcc ataaaatcaa ctgtaaaaga tattgaacag aaaatgaaac aggttaaagg
    35041 tctagccaat gtcaaatctg atttatcgca aacatatgat cagtatgaaa ttaaagttga
    35101 tcaaaataaa gcggcagaaa atggtatttc tgcaagtcaa cttgcaatgc acttgaatga
    35161 aaacttacca gaaaaaacag ttacgactgt taaagaaaat ggtaaaactg ttgatgttaa
    35221 agtcaaacaa aataagcaaa cagactggtc agaagataag ttgaataata taactttgaa
    35281 aaagccgact ggtggtacga ttaaattggg agatatcgct acgttagtta aaacaacgac
    35341 accaagtaaa ttgacgcaag aacaaggtga ttatgcaacg acggtatctg ctaaagtaac
    35401 aaataaagat gtgggtggca caacacgaca agtgatgtct aaaataaata atttggacaa
    35461 accgaataat gtaaaggtta atatcggtgg tgcatcagat gatattaaca atgcaatgac
    35521 acaattagcc tttgcaatgt tagctgcaat cattatcgta tatttaatcc tagttattac
    35581 atttaaaggt ggcttagcac catttacaat tttattctct ttaccattta cagttatcgg
    35641 tgtgattatt gcactattaa tcacaggaga aacaatatca gtaccaagtt taattggtat
    35701 gctaatgtta attggaattg tagtaacaaa tgccattgtg ttaatagacc gtgtcattaa
    35761 taatgagcag cagggcatgg agatgaaaga agcattaatc gaagcaggcg gtactagaat
    35821 tagaccgata ttaatgacgg cgattgcaac aatcggtgca ttagttcctt tgttatttgg
    35881 tcaagatagc tcgattctta tttcgaaagg attagctgct acagttattg gtggtttaat
    35941 ttcatcaact ttattaacac ttgtagttgt accagtaata tatgaaattc tctttacttt
    36001 aaaaaaacga ttcactaaac gatagataat ataaaagcca taaaagcggt catgatattg
    36061 acaactttta tggcttttta aaatttgaaa ttttagacct tatttcacgt gcgatttaat
    36121 cgatgtgtta ataaaacaat actggcattc gatatgtaat ctgatgataa tataaggtta
    36181 caattatgtt attgcatgtt ttgtttatta cttagaataa tacgatggtt aagatgaaat
    36241 atatgacaaa gataaataat gacattaaat tcaagatgtt tacgcctttt agatcttttt
    36301 taagcatttt aaatcctgtc acttcgacat taccaactaa gagtaataag acgataaata
    36361 cccaccaatt ctcttttgga aaactcatcg tcgttaatgc taagacgaat aataacaaca
    36421 cacccataat aattctcaaa atgcgagtaa atataacttc cattttaaaa atactaataa
    36481 aactaacaat tagataaagt attgctagta aaatcagata aaattgcata tgataacttc
    36541 ctctccgttt caaagggttc atgtagtaca gcagtgtaga aaacataaat agcaatattg
    36601 ggatagatat gcgcgtatgt tgttaatgag acactaaatc ataacacgac aacatcttgt
    36661 gattctagat gtaattgagc gaaaaatgaa gcaattcatc aatataacgt attcagagca
    36721 atgctagtca aatttatgta atgaaaagca tttattgtct gaatgataga ttattaatct
    36781 tgcatttgag cacgaacgtt tctaatttca ttttcaatat ctttaagttg acgaacaaga
    36841 gcggaagtat ctaaattcaa agattcccgt ttttctaagt caccttgaag acgaacataa
    36901 tcatatttaa gttcggctag ttggttttct aaagacatac tataacactc ctttgaaata
    36961 taattaagtg catttaatgt aaattatagc atgaaacagt agacttctaa aatatctgtg
    37021 gagtatgtta aagttataaa ttatgaagat tattgaaaga agggataaca gtgacagaac
    37081 aatttactat gattatttta ttaattgcgc ttggttactt tttgaagcgc attaattata
    37141 taaaggcaac agatagtcaa gtcattgcta cattagttct taatgtcaca ctcccatctt
    37201 tagttattgt gaatttaaac agtgcagaat taaaattgtc attctccatt ttaccaattt
    37261 taatgattat atatggtatt gtggcgaaaa tgattgctgt ctggtttttt agaaaatatg
    37321 ataatcacat gcgtgggtca gtaggtatga tgactggtgc tatgaatatt ggattgtttg
    37381 catatccact aattgaagtg atatggccta aaacggggct tatttatttt ggtatggctg
    37441 atataggtgg tgcctttgtg atgtttggaa ttacttactt tgtaggtagc tactttagtg
    37501 aaggtagtga tcaattcgat tttaaatatt taggtaagaa attattacaa tcagtgccac
    37561 tcgttacata cattgtaatg tttatattga atataagtca catccattta ccacatgtgg
    37621 caatcgattt ctttagcata ctttcccatg caaatatgcc gttatcaatg attttgctag
    37681 gtgtcatgtt gaattttact ctggaaagaa agtatatacc tgctactatt aaatatttat
    37741 gtttgcatta tggattagca ttagttgcgg gattgttagt acatgtgttg ttaccggtat
    37801 cagatgatat gattaagacg acattgttag tcacttggat gttcccggtt ggtgtcgcaa
    37861 ttatttctta tggtattcag tttaaatatc gaacactgcc atttataggt atgacgacca
    37921 atttgacaat cattattagt attattattt tgtacgtgta tcaggcagtg tttgtctagt
    37981 ttacacattt ggaaataaaa tccattttag tattcgtaga aaatgagagc cccctttacc
    38041 gcaacatgta aatgcatatt gtgaaaaggg ggcttttatt tatgacatat ttgttcaaca
    38101 ggctgggaca taaatcaat
//
LOCUS       DAPIDM010000011        34667 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000011 DAPIDM010000000
VERSION     DAPIDM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.011"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..829
                     /locus_tag="RJE42_000483"
     CDS             <1..829
                     /locus_tag="RJE42_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV5875936.1"
                     /translation="TKNEKKIAKFILNSPQKVIKMRSQDLASLLDISTSSVIRFSKKI
                     TDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTRTTRALNHANNELNDK
                     TIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQLVQETHIFATLLATHN
                     SNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVAQASNIVLTYGKTDEN
                     EMHMGATTSLFAQMFTIDILYYRYVALNYHASLDYITQSKMALDNYRKHLSNINFKH"
     gene            complement(1218..2597)
                     /locus_tag="RJE42_000484"
     CDS             complement(1218..2597)
                     /locus_tag="RJE42_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5875937.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGIIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            2877..3233
                     /locus_tag="RJE42_000485"
     CDS             2877..3233
                     /locus_tag="RJE42_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875938.1"
                     /translation="MLKQILPRATKISVIFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFVWELIEKNKGGRSS"
     gene            3230..3415
                     /locus_tag="RJE42_000486"
     CDS             3230..3415
                     /locus_tag="RJE42_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875939.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3511..3702)
                     /locus_tag="RJE42_000487"
     CDS             complement(3511..3702)
                     /locus_tag="RJE42_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875940.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(3713..4348)
                     /locus_tag="RJE42_000488"
     CDS             complement(3713..4348)
                     /locus_tag="RJE42_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5875941.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKSEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4423..5340)
                     /locus_tag="RJE42_000489"
     CDS             complement(4423..5340)
                     /locus_tag="RJE42_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDV5875942.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFNLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            5608..6138
                     /locus_tag="RJE42_000490"
     CDS             5608..6138
                     /locus_tag="RJE42_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875943.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6204..7808)
                     /locus_tag="RJE42_000491"
     CDS             complement(6204..7808)
                     /locus_tag="RJE42_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV5875944.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPVAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSSKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDNVDPKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVEDTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8138..8902
                     /locus_tag="RJE42_000492"
     CDS             8138..8902
                     /locus_tag="RJE42_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV5875945.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHVDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHHDISN"
     gene            complement(8960..9466)
                     /locus_tag="RJE42_000493"
     CDS             complement(8960..9466)
                     /locus_tag="RJE42_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDV5875946.1"
                     /translation="MAKFNVENEHVEVEIEKFYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(9636..10946)
                     /locus_tag="RJE42_000494"
     CDS             complement(9636..10946)
                     /locus_tag="RJE42_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDV5875947.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFSWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLVAILFNFPKLKKNTSQEMK"
     regulatory      complement(11023..11118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11171..11743)
                     /locus_tag="RJE42_000495"
     CDS             complement(11171..11743)
                     /locus_tag="RJE42_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875948.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPVYILFTLFVVVAIV
                     SNVWLTHQNVKKHIIKFDKRVAYFLGVLSGVFYIGIVIYGATIIGYYNFISFIIFTIS
                     IVLVQILENILGISIRNTRPSNKQLKVISEQSAQQFKVISLILVSVSIVFIFIALMLK
                     WWLLLVISIIVLIIITVAMIVYEKYYTTPN"
     gene            complement(12230..13111)
                     /locus_tag="RJE42_000496"
     CDS             complement(12230..13111)
                     /locus_tag="RJE42_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5875949.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGYH
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHNDIHGFTTEEFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13220..14341)
                     /locus_tag="RJE42_000497"
     CDS             complement(13220..14341)
                     /locus_tag="RJE42_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDV5875950.1"
                     /translation="MGLKDQAVAWRRYFHQFPELSDKEFKTTQKIKDILTEYHIRILD
                     LPLTTGLVAEVGQGPSCIAVRADIDALPIQEFVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGYGAFKIIETHALKDVQAVLGFHNDPSRPIG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKAPEYFVEFVKRLLHE"
     gene            complement(14617..15855)
                     /locus_tag="RJE42_000498"
     CDS             complement(14617..15855)
                     /locus_tag="RJE42_000498"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDV5875951.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            complement(15855..17516)
                     /gene="hutU"
                     /locus_tag="RJE42_000499"
     CDS             complement(15855..17516)
                     /gene="hutU"
                     /locus_tag="RJE42_000499"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDV5875952.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQSLAITMNHGVAICVDIDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDSGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            17676..18560
                     /locus_tag="RJE42_000500"
     CDS             17676..18560
                     /locus_tag="RJE42_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5875953.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            complement(19113..20048)
                     /locus_tag="RJE42_000501"
     CDS             complement(19113..20048)
                     /locus_tag="RJE42_000501"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDV5875954.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKTSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTGYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFIHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            20326..21585
                     /gene="sdpC"
                     /locus_tag="RJE42_000502"
     CDS             20326..21585
                     /gene="sdpC"
                     /locus_tag="RJE42_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDV5875955.1"
                     /translation="MKKNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDVEKTGVATASTVGIAKNDTENTLADEPSIHEGTEKTES
                     QHHIDNLTESNHDEDHDITSESVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(21651..22337)
                     /locus_tag="RJE42_000503"
     CDS             complement(21651..22337)
                     /locus_tag="RJE42_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDV5875956.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            22508..23161
                     /locus_tag="RJE42_000504"
     CDS             22508..23161
                     /locus_tag="RJE42_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDV5875957.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVRRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNKTLK"
     gene            complement(23397..24416)
                     /locus_tag="RJE42_000505"
     CDS             complement(23397..24416)
                     /locus_tag="RJE42_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDV5875958.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDLTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVENE"
     gene            complement(24446..24772)
                     /locus_tag="RJE42_000506"
     CDS             complement(24446..24772)
                     /locus_tag="RJE42_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV5875959.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            complement(24821..25006)
                     /locus_tag="RJE42_000507"
     CDS             complement(24821..25006)
                     /locus_tag="RJE42_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875960.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            complement(24990..26222)
                     /locus_tag="RJE42_000508"
     CDS             complement(24990..26222)
                     /locus_tag="RJE42_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5875961.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     LNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQIHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            complement(26215..27114)
                     /locus_tag="RJE42_000509"
     CDS             complement(26215..27114)
                     /locus_tag="RJE42_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5875962.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDMSLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKIEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            complement(27345..27992)
                     /locus_tag="RJE42_000510"
     CDS             complement(27345..27992)
                     /locus_tag="RJE42_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV5875963.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNEALIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQPVTSETPEKAGTSETPKTPEETDNQL"
     gene            28391..28999
                     /locus_tag="RJE42_000511"
     CDS             28391..28999
                     /locus_tag="RJE42_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDV5875964.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEDG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            29336..30544
                     /gene="gltS"
                     /locus_tag="RJE42_000512"
     CDS             29336..30544
                     /gene="gltS"
                     /locus_tag="RJE42_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV5875965.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISIIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(30592..31641)
                     /locus_tag="RJE42_000513"
     CDS             complement(30592..31641)
                     /locus_tag="RJE42_000513"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV5875966.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMTYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(31672..32619)
                     /gene="corA"
                     /locus_tag="RJE42_000514"
     CDS             complement(31672..32619)
                     /gene="corA"
                     /locus_tag="RJE42_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV5875967.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFSFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            32716..33207
                     /locus_tag="RJE42_000515"
     CDS             32716..33207
                     /locus_tag="RJE42_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV5875968.1"
                     /translation="MVIITQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYN
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            33204..33623
                     /locus_tag="RJE42_000516"
     CDS             33204..33623
                     /locus_tag="RJE42_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875969.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(33731..34651)
                     /locus_tag="RJE42_000517"
     CDS             complement(33731..34651)
                     /locus_tag="RJE42_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5875970.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFKAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQFLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
BASE COUNT        11458 a         6650 c         4870 g        11689 t
ORIGIN      
        1 gactaaaaat gaaaaaaaga tagctaaatt tatactaaat tcacctcaaa aggtgattaa
       61 aatgaggtct caagacttag caagcttatt agatattagt acatcatcag tcattcgatt
      121 cagcaagaaa attactgatg gcggttttca cgacttgaaa ataaatatat ctaaatatgt
      181 tcctaaagca tcatcaattt acaatgttga attaatgaat aacgaaagca cagaatcttt
      241 aagaactaaa ctccatacac gtactacacg cgcacttaat catgcaaata acgaattaaa
      301 tgataaaact attgatcaaa tatgtcattg tttaaaacgt tctgagacga tttttatata
      361 tggttttggc gcatcttttg tagttgctac cgacttatac caaaagttat caagaatagg
      421 tcttaatatt caacttgttc aagaaacaca tatttttgca acattattag caactcacaa
      481 ttcgaacgat agtgttattc ttattactaa caatggtaca caaagtgaaa tgcaatcaat
      541 ggttaaagtt attgatgact accatatacc tataatcaca attactagta caagggataa
      601 tcccgtagca caggcatcaa atattgtttt aacttatggg aaaacggatg aaaatgagat
      661 gcatatggga gcaacaactt cattatttgc tcaaatgttc actattgata ttttatatta
      721 tcgttatgtt gcattaaatt atcacgcatc attagattac ataacacaat ctaaaatggc
      781 tttagacaac tatcgtaaac atttatcaaa tattaacttt aaacattaac agtcttatat
      841 aaattcttta atcatgtcat ataataattt atgcatcatt ttcaaagctt acatgtcttt
      901 tatcttgtat aacatacgca tatgaccaat gatttgataa ggccattcta tttcttatat
      961 taaaaacaaa cgcagttgat taacggattc attccatata tcaattgcgt ttgttatttt
     1021 aataatgcta ttttttattt taaagcccac agtaaaaact atcgattgta taccctttta
     1081 aaggtaaatt cttactacaa cttcgattta agctatattt ataaaaaaat gcgcctactc
     1141 ataacacttc tttggctatt taactttaat aagctagggc caagtttgtc ataaaataag
     1201 cgcacattat aatcatacta ttttgctttt acattatttt tattatatct aataaaataa
     1261 taaatcgtta caatcgttaa gaagatgaca ccaataagca ctgatattct agtttcaaca
     1321 ttaaatacca taccaaccaa tactaatatt aagaataaga ttgttaagta gttacttact
     1381 gcgccaccag gcattttgaa tgggtgacct tccacttctt cagggtgtaa ttttctaaaa
     1441 cgtaagtgac tgaataaaat cataaaccaa ggaatcatac ctggtaaaat agacgcacta
     1501 tatacgtata cgaaaatact cttcgctcca tcgatatata aaggtaaaat tacatttaat
     1561 aaagcaccaa ttaaaatacc aagagatact gcaacaacag tgtacaatgg cacaccattt
     1621 ctcatgattt tagtaaatat tttcggcatt tcacctttat gagcaagtgt ataaatcata
     1681 cgactcgcac tgaaaatacc tgagttacat cctgacattg ctgcagttaa tacaacaaag
     1741 ttaattaatc cagctgcaaa tgtaataccg attttagcaa atgttgcaac aaacggactt
     1801 ccaatgtctc ctaattgatt ccaaggatac acagaaacaa taacaaaaat agcacctaaa
     1861 tagaaaatta aaattctcca gataacacca ttcactgctt taacgatatt tttctgagga
     1921 tctttagttt cacctgcagt aatacctatc agttccacac cttggtatga accaattaca
     1981 attgataatg caaagaagaa accaacaata ccatttggca taaatccgcc atttgtccat
     2041 agattagaaa tacctaccgc atggccacca tttccaaaac caaagaaaat aagaccaaaa
     2101 ccagcaataa tcattaaaat aattgttaca actttaatta aagcaaacca aaattcaaat
     2161 tctccaaacg cttttaccga gaataaattc gctgccatca ataataaaat agcaataata
     2221 ccaggaatcc aggttggcaa ttccgggaac caaaacttca tatattctcc tactgcgatg
     2281 acctcactca taccaacaac aatccattgg aatatattac tccatgctgt catataacct
     2341 gctgcaggat gtatataatc acttgcaaat gttgcaaatg atcctgttgt agggtttaaa
     2401 taaatcattt cccccattgc tctcatgatt aaaaataaaa agatacccgc aattaaatat
     2461 gcaaggataa ctgatgggcc tgtccattta attgtacttg tggcacccat gaaaagcccc
     2521 acgccaatcg taccaccaag tgcaatcatt tgaatttgac gggcactcaa tcccctttgt
     2581 agttcattgt tctccataac ttcttacccc atctctgatt gatactaaat aaatccataa
     2641 aaaaacttat tgccgtaatt atactataaa ttatcgattt tggtacatta atatttgcat
     2701 accatgcaat cgtttacaga actactatag caggcttttg caactttgca aacaattatt
     2761 ttgtgatttt tctaaaaaat taaaaaagtt gtccatgaac cgttgggtca catacaactt
     2821 cagctattac aagtaattat gatataattt taataatatt aaaaaggagt atatcaatgt
     2881 taaaacaaat actgccacgt gctacaaaaa tatcggtaat ctttgctgta gcatttttca
     2941 tcataaatta tatcggtatg gaaaaacctg acatcctata tttagttgga agaactatta
     3001 ttgcaacact tgctttcata ctcatttgtc ttacattatt tactattatt aattctccag
     3061 agcgcaagat taaactcggt acgactttac cgattgcatt aattattggt atcattttcg
     3121 gcgccatatt tttaacggta caaatcggtg tcatcactgg gttaatcatc ggtgtgattg
     3181 ctacgtttgt ttgggaattg attgaaaaaa ataaaggagg tcgttcatca tgacaacagc
     3241 attaattggt gtaattgtat taatcttact aattattagc tttgtgccaa actaccgtgc
     3301 tatgaaatca gcaaaagaac aaggaagtaa tgcaacaaga gctacaatta tggtaggcat
     3361 agatattatc ttaattgtac ttatcatcgt gacgttatta ttaaaatata tgtaactatt
     3421 taaaccaccc tatctcatac ataaacatga acgttctacc atttcacata catatggtcg
     3481 tccatttaat gaggtaaggt ggttttctcg ttaaagttta ccttgtaata gtaaaaaata
     3541 gccaattgtt gcgagtatta agtaaatcat tgaaccaaca acaaaagatc ttacttgcac
     3601 gcgaaatggt gtctgtttgt taaatatagg accaatcacc aaatttccaa cgcctaaaat
     3661 tactggacca gctagaaaaa acaatataat gtaacccatc ataatacgcc tcctatttcg
     3721 taaagaaacg cgctacaatt tgatcgtaat caatatcttt tgctacataa tctacattag
     3781 aaaagtcctg tgcgcttgtc attttattcg tattaacaat aacatctaat ccagctgcaa
     3841 tcgcggctgt tgcaccattt acagaatctt caatagccaa acattgtgtc ggcatataat
     3901 ttaattgttg tactgcagat aaatataatt cagggtcagg tttaacttgt tcaacatctt
     3961 ctctaccaac aactacctct atataatcgt ctagacctaa tgctttaaag gtaggcatta
     4021 tttccgattt aacactactt gtggcaatcg ccataggaat gtgacgttgc tttaaaaatg
     4081 ccatcaatga tttaatcgta tcaatcatcg gtaatgttgt actagtaaga tgatgttctt
     4141 cataaagttt atctttattt tccgaaccaa tcgctttaat taaatggtca tgcaaatctg
     4201 tagctgctcc tccaatagaa gaacgataaa aatcaatgct tataggatcg gcattatgca
     4261 tctctaaatg tttattaata acattaaata aatgttgttc cgtatctatt attgttccat
     4321 cgaaatcaaa tataactgct ctatacatat atttactcct ctattttttc tctttatcaa
     4381 agcttaacat agtccgatga ccacttcatt ctattagtgc aattataact tttttgacca
     4441 atactttgct aaaattggcc ctgttatatt atgaatgaaa ctaaacactg cgcctggtac
     4501 tgctgcaatt ggattaaaat gcaatgctgc taatgacaca gctaaaccag agttctgcat
     4561 tccaacttca atactgactg ctttttgatc ttgtcgatct aattttaaaa gacgagctaa
     4621 ccaatatcca atcgtatacc ctaatacgtt atgtaaaata actactaaaa atattaataa
     4681 tcctgtagtt aagatttggt gcttactgcc acctacaact attgctaaaa ttaatgaaat
     4741 agcaacaact gatataatcg gcaaagctgt agaagctttt tcagcaattt tacgattaat
     4801 aatttgcaaa acaataccta aagcaattgg aattaataca acttgaacaa ctgaccacaa
     4861 catactcaag aaagatactt ccaaccattc atttgcaaat agatatatta acgcaggtgt
     4921 aacgaatggc gctagcaacg tagagaccgt cgtaatagaa acagaaagtg ctacgttagc
     4981 tttggctaaa taactcatta cattacttga tgtcccaccc ggacaacatc caactaatat
     5041 tacgccaaca gcaatatcag gtggtaaatt aaaagacttt gcaattataa atgctaatgt
     5101 gggcataata ctgaattgta gacagacacc aataattact gctctaggtg ctttaaagac
     5161 cattttgaaa tcattaggtg taattgttaa tcccatacct aacataacaa taccaagtaa
     5221 ataaggtacc cacttaccta atgtcgcaac atgttgtggg aaaataaatc caataacagc
     5281 tgctactaac atccatacca aaaaactctt cgtggcaaat atactcaatt tttgaaacat
     5341 tgttaacaca tcctcgtcga taatattgaa aaatattacc ataagaccag taaggttaca
     5401 attacttttc tgaaaattaa gattatttag ccattctctt tttattgcaa atattttatc
     5461 actgataaac aattatttta aacttcattc aaaagtctta gtgaataaag aaaaaaagcg
     5521 tggtaaaatc aacttgaaca aaattaagtc acctttataa atattaaacg tgtatttaat
     5581 gccatgtatt cctcgggagg tcctcacatg ttaattgata aattcgaaac atacatcatc
     5641 aacatcgccg gtttaaatga ccgtactact agaaaaaaat taagtaagtt atgcaaaagt
     5701 gtacaatttt gtgatgcgtt gcaattctca ataaacaaac agtttaatca gtatgtatta
     5761 gaaattagct taccaaagca acaacttcca tacttcattt catttttaag ttttcatcag
     5821 tatagtattt tccaagtact ttcaccaaag aaaataaatg aattacttga ttctgataat
     5881 ttatatcaat ctgcaaagcg tttcgatatc aatattgatg gcttgcaaga tgctttcatt
     5941 aaagacaaag tcatcgatat tatgaatatg ttccaaaatc acactgatat cacttatacg
     6001 ttgaataagt ctcatgcgca tattatatgt acgccagaaa tattcgctaa attgttacat
     6061 acgattgcaa ctagaaatat cgacattcta tctgccaatt atagatcgtc atctatgagc
     6121 aaagctcgta tatcataata aaagatacat ctatacgatg atcatttaca atgattgcgt
     6181 atagtgatgt gtcttttttt gtattattta tcttcggacg attgatcctc aaccatttgt
     6241 tcaaatgctt cacgaacttg tggtactgtc atacctacta caacttggat actcttacca
     6301 ctctttacta atccgtgtgc catttgttga tgcgtgaaat attccgtatc ctcaacttta
     6361 ctttcatcat aaacagttaa gcgtaatctt gttgtacagt tagtaacatc tttgatattt
     6421 tctttgccgc ctaaaccgtc taggtaatac gcagctttgt cttcgtattc attaccagtt
     6481 gaagcacgtt tagggtcaac gttatcacct tttttatttt tatagtcttg tttagagaat
     6541 aacttaacct cttcttctgt tttctttcgt ccaggtaatg gaatatcaaa tttcaaaatt
     6601 aagaatctga ataagaagaa gtaaatcgct acaaatatca gaccgataat aacttgtgct
     6661 acataagtgc cccaatgttc ttttccaagt ggtaaccagt ttgttgaaat aaagtctaac
     6721 aaaccaccac ccatatttcc tacaacacca aatgcgtaca ttaaagtatc cattgatgct
     6781 gctaaaactg catgtaaaac aaataagtat ggcgcgatga ataagaatgt aaattctaat
     6841 ggctcagtaa tacctacaac gactgctgtt aatgttgcag gaataagtaa tgctgctact
     6901 tttttacgat tttctttagg tgtcgtagca tacattgcta aggcaatacc taaacatcca
     6961 aatactttcc catttccttg aagcatgaag ccataaggga attgctcttt taatggctta
     7021 ctgctcttag caaactcatt taagtgttgt aaccattcag ctttcaagcc gtgattaact
     7081 accactggac caacttcaat tggtgcataa ataaagtgat gtaatcctgt tggtattaat
     7141 acacgttcta agaagtggta caaccataca ccgatatagc ctgaagcgat gataaaatgt
     7201 tgcattgaac caataccgtg ctgaacagtt ggccaaataa cacaagtaat tgctgctact
     7261 ggtaacatca caaaaaatga gattgtaacc acaaatgtta aaccttggaa tacaccaacc
     7321 atttctggta atcgtttact atagtatcta ttgtgaatcc aagtaataat agctgaaata
     7381 ataataccac ctagaatatt cgtatccaat gtggcaatac ctgcaattga ttttaaacca
     7441 ggtacatttt caacgccttt ttctaaatta gcgccaaacg tatgtggcca ttgcgttaaa
     7501 atggcattta taaatgtatt aaacattaag taacccatca atgctgcaag tgctgcatga
     7561 cctggtgctt ttttagctaa agaaagtggt aaacctacaa caaatacaac ttccatatgc
     7621 gtgaaaataa cccagccacc agattctatc actgaccaaa atttaaacca aaacgtatgt
     7681 tgatctgcta aactccccat gattgtagga tttttaaata atgtcgcaaa gccaagaact
     7741 atcccaaaga aagcgaacat taatactggt acaatcattg cactaccgaa acgctttatc
     7801 gcattcatct tctattccct ccatatcatc tttcctaaca atacatctaa ttagattcat
     7861 ttataaatag atgccttact atttaaatat aatatatagt aaacgcttac aaacctacaa
     7921 cgacattgac gtattttgaa agtattttgt ataatcagat tatcttttca tatagtgaaa
     7981 attttttcac aaccttatat atgacatcgt tgtatttgta ataaattcgt tttaaaagca
     8041 taatcaaacc tatatcaata cacaaatata tataatgaca tacaaggttt taatgtaata
     8101 tcgatctatc acacatttat tttcaaggag gttgaatatg tttttagatg aacacattaa
     8161 tcgaaacttt gataaactta atgacaatga tttacatatc gcccacttca tcaacacaca
     8221 cgttgatgaa tgtaaaaata tgaagataca agatttagcg caattcacac atgcctcgaa
     8281 tgccacgatt cacagattta cacgcaaatt aggttttgac ggttatagtg attttaaatc
     8341 gtaccttaaa tttgaaagta ataagacaca ccaattacca tctaactcga tcgatagttt
     8401 caaacaagaa atcgatagta cattcagtta tttagaacgc gttgattacc agcttcttac
     8461 tgaaaaaatg catacagctt caacaatata tttgtatggt acagggcgtg cacagttaaa
     8521 tgttgcttca gaggcacagc gtatattgtt aacgttacat aatcatgtca ttgtacttca
     8581 tgatgaacat gaacttaaaa tgattttgaa caagagtgag cctaatgatt tattctttat
     8641 tatttcatta tcaggtgaaa cacatgaatt attagaagta acaaacttgc ttcaacttag
     8701 acaaaaatac tttatttctg ttactacaat gaaagataat acccttgctc aaaaagcaaa
     8761 ctacaacgtc tatgtatcca gtaacacatt ctacttaact gatggtacag actattctag
     8821 cttcatcagt tatcatatat tctttgaaac acttgtaaga aaatacaatg agtatttatt
     8881 acatcatgat atttcaaatt agaaaaaagg aagttatgat ttaatggcct tgagcctatc
     8941 ataacttcct tttatcattt tattgttgtg ttgatgtttc gataacgtgg tacatcttat
     9001 caaacatcaa ttcgaaacca tgcaccattg catcatgata ttcttttttc ttttgcttgt
     9061 attctaaatt agtaaatcgt ctttcttttt caactaatga acgataataa aatagcattt
     9121 gggtgccacc tgtttcacgt tcaaaaaatt ctacctcaat gacatcttgt gtttcactta
     9181 gtccaggcat accgatagtc atcttaacgt attcatccat aactaaagat tcataaatac
     9241 cttcaatcac atttactttg ccattacgtt gttgatctac aatacgatat ttaccgcctt
     9301 ctttaacatc cgcttcaatc tctttattcg ttctggctga tgtcataaac cattgtttca
     9361 acaaatcttt ctttgtccaa gcttcgtata ctaactctgg agaaaattta taaaactttt
     9421 caatttcaac ttcgacatgt tcattctcta cattaaattt tgccactgtt gtccacccac
     9481 tttcgctctt acttttattt taacgtattt ttgctcagtt ccaaacatag atgatcatca
     9541 tttttaaaag attagcgtta tacggtgagt acaacatgat ctgttaatat aacaagccac
     9601 cttacttggc tacatcgata tattgttaag cattactatt tcatttcttg actagtgttc
     9661 ttttttagct ttggaaaatt aaataaaatc gcaacaagtc cgcatacacc taataatata
     9721 ggataaatgc tgtatgggaa taacattaac ggtgaaatac cagctacacc agccgctgaa
     9781 ataacttgcg ggctatatgg taataaacct tggaagcagc ctccaaatat atcaagaata
     9841 cttgctgatt tccttgaatc tacatcatat tcatctgcaa tatttttagc taaaggacct
     9901 gacataataa tagagatggt gttgtttgcc gtggcaatat ctgcgacact taccaaactc
     9961 gcaattccta attctgcgcc acgctttgat ttcactttag agcgaacaaa ttgcaacaac
    10021 cattcaatac caccattgtg ttgaataata ccgactaaac caccaattag caacgcaatc
    10081 atagcaatat cttccatgct tataatacct ttggacactg catctagtag ccccatccaa
    10141 ctgaatgaac catctatgag accaatgatt ccggctaata atgttccgcc aatcaatacg
    10201 ataatgacat ttacacctaa taatgctaat accaatacta aaatatacgg tacaacttta
    10261 attagattat aatcatagtt tttagcatga tttaaagaaa tgccattcgt taagaaatac
    10321 agaataataa tcgttaaaat agcacctggc aatacaattt taaagtttac tctgaattta
    10381 tctttcattt tcgtatgttg tgttctaacc gcagcaattg ttgtatctga aatcattgat
    10441 agattatcgc cgaacattgc acctccaaca actgtagcca ttgctagcgc agttggtaca
    10501 tctgtcgctt gtgcaaatcc gtaaccaaca ggtgctattg cagcaacagt ccctactgac
    10561 gttcccatag atatagaaac aaacatacaa atcacaaaca atcctacaat aattaaattt
    10621 tctgggatta atgatagtcc taaattaact gtcgacttta cgccacccat tttttcagct
    10681 gtatttgaaa atgcacctgc taaaataaaa atcaacatca ttaaaataat gtttgaatgg
    10741 cctgcacctt tcgtgaaaac ctcaactttt ttagcaaatg attcttttcg attcattaat
    10801 aacgccacaa ttaccgttat cgtaattgca acatttaatg gcattgaagt aaaatcacct
    10861 gtgataatac ctacgcctaa aaacaacgcc acaaataata acaaggggaa taatgcccaa
    10921 gcattgctct ttttatgtac ttccatcctt tttacctgct ttccaattaa aaatacctct
    10981 ttctcacaaa cgatgaagaa agaggttttc atgtacttta cctgcttatc ttcaaaccat
    11041 tacggttact ggaattggca cattcgagat gttgccgagg cttcataggg ccagtccctc
    11101 cacctctcta gataagtgat gcttatttac gtttacgtta caagataatc cttagtacgt
    11161 caatcataaa ttaattagga gtcgtataat atttttcata aacaatcatt gctactgtaa
    11221 taataatcaa aacaataatg ctaataacaa gtaaaagcca ccatttaagc attaatgcaa
    11281 taaaaatgaa cacgatagac acacttacta atattaatga tatgacttta aattgctgtg
    11341 cgctttgttc agaaataact ttcagctgtt tgtttgatgg acgcgtattt cttatactga
    11401 ttcccagtat attttctaat atttgaacca atacgatact tattgtaaat ataataaatg
    11461 atataaaatt atagtaacct ataatagttg ctccataaat aacgattcca atgtagaata
    11521 ctcctgagag cacacccaaa aagtaagcaa cacgtttgtc aaacttaatg atatgctttt
    11581 taacgttttg atgtgtaagc catacattcg aaacaatcgc aaccactaca aacagtgtga
    11641 ataatatata tactggcaac ttttgttcag gaaaaacagt cgctattcca aaagctaatg
    11701 ctaaaatcaa aaataatata gctctagata ctattaatgc cataataaca acccctttgt
    11761 ttaatatcga gtttgcaaat ttacgtttat cagcatttct atgatcagta cttctacggg
    11821 cagcgtttct atgtaattta catcatctta acatataaat actttgctat ataattgaaa
    11881 acatatccta ttattctttg tccgttctga cgtctaatat ctaaccttag gcatttcact
    11941 tggtaatgaa tttaactttc ttccactaac cgtccctaaa cccaatccca caacagtttt
    12001 taactttttc gttgctgtcc tgacatcctc attaagaaag tttattctgc ttaaaactta
    12061 taatccacac cctgagcaaa cgctccttat gacaaagtat taaaataagc cgataaagat
    12121 acacaccttt accgactatt taaaatacac ttcaccaatt cattttaatt tacttgattg
    12181 aagtagctaa attaatatta tgttgttcaa ttaaacgctt catacaaacc taatctattt
    12241 gcactccacc gctaacaccg aacacttgtc cagttgtata acttgattct tctgacgcta
    12301 atagcacata agttccacat aactcaacag gttgacctgc acgacctaaa ggtgtttttt
    12361 gaccaaatgt tgggatttta ctttgaggtt gtccaccaga aatttgtaat ggtgaccaga
    12421 atggaccagg cgctacacag ttcactctaa ttccttttgg tcctaattct tctgaaaaac
    12481 ttttagttaa tgaaataatt gctgcttttg aagcggcata atcatgaaga ataggactag
    12541 gattataacc ttgtacagat gatgtcgtcg taattgacgc acccggtttt aaatattcca
    12601 aagctttttg aactgtccaa aatagcggat agacattcgt ttcaaatgtt tctgtaaatt
    12661 cctcagttgt aaatccatga atatcattat gatactgttg atgtccagca actaaagtaa
    12721 cattatctaa gccacctaat tgttgatatg cttgttcaac aaggtcataa ttgaactgct
    12781 catctcttat atcaccagga attaacactg ccttttgacc actttcttca atcacttggc
    12841 gtacttcttg tgcatcttgt tcttcactcg gaagatagtt aatcgctaca tctgcacctt
    12901 ctttagcata cgcaattgct gctgcacgcc ctattgctga gtcaccacct gtaactaaca
    12961 ttttatagcc ttgtaagcga tgataacctt ggtaagacgt ttcgccacaa tcaggtgctg
    13021 gcgtcatttc agattgtaaa cccggtacct cttgttcttg tttttcataa tccgttgttt
    13081 taaattttgt tctaggatct tgagctgcca tttttaacat ctccttattc gcttaatggt
    13141 tattatttac ccaatcttcc caggaactta atcatgatta cgctaaaaat tactttcttc
    13201 tttaaaaaac aagctcgaat tactcatgca atagtctttt tacaaattca acaaaatact
    13261 caggtgcttt ttccaaaatc ctttcatccg gtttatattg aggatgatgt aaatcatatt
    13321 cactatgaga accaattaac gcaaatacac ttggaaaatg ttgactataa cctgaaaaat
    13381 cttctccaat cgtaagcggc tgttccatca tttccacctt atatccaaca tgttgggcta
    13441 ctgcaattgc tttatgcgtc aatgcctcat cattcatcac agcgccaggt aaatgtgtat
    13501 aatttaaatt aattttcata ttatatgctt gagctaaccc gtccgcaata tcttgtaatc
    13561 gtgtttctac aagctttcgc accacaggat caaaactacg cactgtgcct tgtacatacg
    13621 catgatcagc aatgacattc caagtattac cacatgatat ttgtccaatt gttactaccg
    13681 cttcatcaaa cgcagataga tttctactaa ctatggattg aatactatta atcaattgcg
    13741 ccaacacaat aactggatcg ttgcattgtt ctggctttgc agcatgacca cccactcctt
    13801 taatatgaaa ctcaaaacga tctactgctg atgtaattgc ccctgttttg attgcaaatg
    13861 tacctatcgg acgcgatggg tcgttatgaa aacccaatac cgcttgtaca tcttttaatg
    13921 catgtgtttc aataatttta aaagcgccat atcctagttc ttctgctggt tgaaaaatga
    13981 atttaacacg cccagtaaga gtgccctcaa tttcttttaa ctttacagct gtagccaaaa
    14041 tactagccat gtgaatatca tgaccgcacg catgcatcac accttcattt tcagatttaa
    14101 aatcctgttc aacaaactct tgaatcggta aggcatctat gtcagcccga accgctatac
    14161 aactcggtcc ttgtcccact tcagcaacca gcccagtcgt gagcggtaag tctaatattc
    14221 taatatgata ttctgttaaa atatctttaa ttttttgtgt agtcttaaat tctttatcgg
    14281 atagttctgg aaattgatga aaataccttc tccaggcaac agcttgatct tttaatccca
    14341 tcggtcattc cccttcctta agtcaatgat atgttgtcta ccctacgatg atcatcttta
    14401 actattaaac gatgatttca caacaatgta ctcttgttaa ttgctttcgt taatgataga
    14461 cagttgttta ataatatcgt aacactattg tcaaactatt ctaactttta taattaagaa
    14521 cctatacaaa aacgtgttct cggataaact tgtttttaca aaccacaaaa agctctaaac
    14581 attagtgtaa accaatgctt agagctttta attactttat gctttaaaag ataatgtgtt
    14641 atctacgatg accttaccgt ctttaataac tttttctgcg tgattgatac caaaatgata
    14701 aggaatatat tcatgatttg gtgcatccca aattactaaa ttagccttat cacctgtgtt
    14761 aattgtaccc gcgttaatgt ctattgcttt agcagcattg accgtaacag cattccaaac
    14821 ttcattaggt gatagcttta atttcaacgc tgcaatcgcc ataacaagtt gtaagttgtt
    14881 tgtgacacta ctaccagggt tataatcagt tgctaatgca atcgcaccgt tattgtctag
    14941 catgcctctt gcatctgcat aatcttcttt acctaaatag aacgtcgttg caggtaagag
    15001 gacagctaca gtatcactat ttcgcaactt ttcttttcct ttatcactag aagctactaa
    15061 gtggtctgct gatattgctt gttcatcaat tgctaattcc agtccgccta acggatcaat
    15121 ttcatccgca tgtattttca ctttaaaacc tgcttctttg gctttttgca tataatgttg
    15181 cgattgttct attgtaaata cacctgtttc acagaaaata tccgcaaaat ctgcatattg
    15241 ttttacttcc ggaagtaacg caatcatttc ttctaaaaat gcctcatttg aacttgcctc
    15301 tttaggtaca gcatgaggcc ctaggaaagt atgtttcatg tctaaatcat atttctcagc
    15361 taaacgatta gacactttca attgcttcag ttcattttct ctatctaaac cataaccact
    15421 cttactttca actgcaagca cgccgtgttt aatcatagta agcaaatcat gctctgcttt
    15481 tttaaacaag tcatcttcgg atgtttctct agtagcatta acggtagata atatgccacc
    15541 acccatttct aatatttcaa ggtaagactt accttgacgt tttaatgaca tctcatgttc
    15601 tcgagatcca ccaaatgtta aatgggtatg agcatctact aatgccggag aaactacttt
    15661 tccactagca tcaatcgtct cagtcgcatc gtagtcatct gtatgttgtc cagcatatac
    15721 aattttgcca tctttaatga caactgtacc atttttcaca acatttaatt catctaattc
    15781 cttacccttc aaaggtttat ctgttgatct cggtaaaatt aattctgcta tatgattaat
    15841 tattaaatca ttcattactt atcacctgct ttatcaatca ttggaatatg aatacctttt
    15901 tctttagctg tttgaatagc gatgtcatag ccagcatcaa catgacgggc aacacccata
    15961 cctgaatcag tagtcaatac gcgttccaat cttctttcag cacgctctga tccatctgct
    16021 acaacaacca tacccgcatg aagtgaatat cccatgccaa caccgccacc gtgatggaat
    16081 gaaatccatg aaccacctgc agctgtgtta atgagtgcat tcaatacagc ccaatcacca
    16141 accgcgtcac taccatcttt catactttct gtttcacggt taggactagc aactgaacca
    16201 gcatctaaat ggtctcgtcc aataacaatt ggtgctgaaa tttcaccgtc acgtacaaga
    16261 cgatttaaag ctaagcccat tttcgctctt tctccatagc ctaaccaagc aatacgtgat
    16321 ggtagtcctt gatatgaaat tttttcttca gctaaatcaa gccatcttaa taacttttca
    16381 ttttctggga aaagtttgcg catttcttca tccgcacgtt cgatatcttt tggatcacca
    16441 ctcaacgcag caaagcggaa tggcccttta ccttcacaga ataatggtct aatgtaagct
    16501 ggtacaaagc ctgggaagtc aaaagcattt ttcactccgt tattgaaggc tacttgacga
    16561 atattgttac cataatcaaa tgctacagcg ccacgttttt ggaattcaag cattaattca
    16621 acatgctttg ccattgaagc ctgtgacagt tcaacatatt ttttcggatc tttttcacgc
    16681 aatactttcg cttcttctac agagtatcct tgtggcacat atccatttag cggatcatgt
    16741 gcacttgttt ggtcagtaat aatgtcaatt ttaaatcctt tttctagaat agcttgatgg
    16801 atgtctacag catttccaac taacccgatt gataatcctt ctccacgttc tttcgcctct
    16861 tctgctaatt ttaatgcttc atctaaatca gctgttttaa catcacagta tttcgtatca
    16921 attcgcttat caacacgtgt ttcatcaata tccacgcaaa ttgctacccc atgattcata
    16981 gtaattgcta acgattgcgc accacccata ccacctaaac ctgctgtcag tgtaacagtg
    17041 cctgctaaat ctccattaaa gtgttgatta cctagctcag caaatgtctc ataagtacct
    17101 tgcacaatac cttgagaacc aatatatatc caactaccgg ctgtcatctg tccatacatg
    17161 attaaacctt ttttatctaa ttcattaaaa tgatcccagt ttgcccattc aggcactaat
    17221 actgagtttg aaattaatac acgtggcgct tcttcatgtg ttttaaatac agcaactggc
    17281 tttcctgatt gtactaacat tgtctcatct gattctaatt cgcgtaacgt tttctctatt
    17341 gcttcaaaag cttcccaatt acgagctgct tttccaatac caccataaac aactaaatct
    17401 tctggtcttt cagcaacttc tgggtctaaa ttgttgtata acattctaag tactgcttct
    17461 tgttcccaac ctttacactc aatactcaaa cctttttttg cttgaatttt tctcataaaa
    17521 ttcgctcctg ttctttttag aagttaattc cactaaattt aaaacgctta cattattatc
    17581 ttcaatattc attatagtat gttaaaatat agccaacaaa tataaataaa ctaattatcc
    17641 atagcttgaa tctataaata aaaggagcaa aaaacatgaa aattattcag ttagaatact
    17701 tcttggctat cgtgaaatat aatagtttta ctaaagctgc acaattttta catattagcc
    17761 agccatcttt aactgctacg attaaaaaaa tggaagcaga tttaggttat gacttattta
    17821 cacgttcaac aaaagacatc aagattaccg aaaaaggaat acagttttat cgttatgcga
    17881 gcgaattagt tcaacaatat cgatccacga tggaaaaaat gtatgattta agcgttacat
    17941 cagaaccaag gataaaaatt gggactcttg aatctacgaa tcaatggatt gcgaatttaa
    18001 ttcgaaaaca ccattccgac taccctgaac agcaatatcg tttatatgaa atacatgaca
    18061 aacatcaatc tatagagcaa ttactgaatt ttaatattca tttagctata acaaatgaaa
    18121 aaataaccca cgaagatata agatccattc ctttatatga ggaatcttac attttattag
    18181 cacccaaaga aacatttaaa aatcaaaatt gggtagatgt tgaaaatttg ccactcatat
    18241 tgccaaacaa aaattctcaa gtgcgcaaac acttagatga ctattttaat agaagaaata
    18301 ttcgtccaaa tgtcgttgta gaaacagatc gattcgaatc agcagttgga tttgttcatc
    18361 ttggcttagg ttacgctatc attccgagat tttattacca atcatttcac acgtccaatt
    18421 tagaatataa aaaaattcgt ccaaacttag gccgaaaaat ttatatcaat tatcataaaa
    18481 agcgtaaaca ctccgaacaa gtacatacat tcgtacaaca atgccaagat tatttatatg
    18541 gacttttaga ggctctttaa cttaagttat tagagcctct tatgcagttg cccagtcaac
    18601 tgtatacctt ttgcatttaa cttaagttat tagagcctct tatgcagttg cccagtcaac
    18661 tgtatacctt ttgcatttaa cttaagttat tagagcctct tatgcagttg cgtagtcaac
    18721 tgtatacctt ttgcatttaa cttaagttat tagagcctct tatgcagttg cccagtcaac
    18781 tgtatacctt ttgcctttaa cttaagttat tagaacctct tatgcagttg cgtagtcaac
    18841 tgtatacctt ttgcatttaa cttaagttat tagagcctct tatgcagttg cgtagtcaac
    18901 tgtataagaa tttcataacc aattttatga tttccatgta taaatcatta cattcagacc
    18961 agcaaacata aataagcagt aagatgacta caacttaaga gtcttcttac tgcaattatc
    19021 tttcaaatat atcaacgtta atataacttc tattacgaaa tactcacatt ctgccctgca
    19081 atgcaaattt cgtcacatat aaatattttt aattatttta aaaaatgatg cactaaatta
    19141 gcaacgagct tcgcagttct attgtcagcg tcatatgttg gattcatctc agcaatacta
    19201 actgaagaca ccttatcact tggaataata cgttttgcca attcaagaac agtatgtgga
    19261 tacaaaccta acactgccgg cgcacttacc ccaggcgcaa atgcactatc aatgacatcc
    19321 atacaaatcg taaacataat gacatcatgt tcatgtataa aacgctcaat catatcttta
    19381 attgttggtg atacgtgact caataattca tctgcaaaga cataatcaat ctttttctct
    19441 ttcgcataat caaataaact ttgcgtattc ccaccttgag caataccaag cactaaataa
    19501 cctgtgtttt catcttcttc taaaatttgt ctaaagctcg ttccagatgt agattgttgt
    19561 tcagcacgtg tatcaaaatg cgcatcaata tttatcacac caatagattg tgttggatag
    19621 actttacgtg ttgctaaata ttgagcatac gcaatatcat gtccaccacc taataaaaac
    19681 gtttgtctat gattaacaat tgacttcgct gcaagtgttg caaattcttt ttgagtatca
    19741 attaattcct catgatcatg ataaacattt ccgtaatcga ctaaagtttc acattgattc
    19801 aaatccggca aacctgcaaa tgcttgttta atcgcatctg gcccttcttt tgcaccaatg
    19861 cgccccttgt ttaaagcaac acctttgtca acagcatagc ctaatatacc aacccctgat
    19921 ggtgtactag ttttttccaa tttcgacaaa tcttcaaatg tcactgtttg aaaatgcctg
    19981 aattttttcg ggtctgtttc actatctaac cttccagtcc ataaatttgg ttcaccttgc
    20041 ttgtacacag catttccccc tcttatttat gtggcttttt aacaattaaa gtataacgta
    20101 ctacaaattt tgaattcaat tcataattaa aaagtactta tctatatacc ttttacccct
    20161 attttctatt cactaacgaa cacgaaagcg atttatgtta ataaaatacg tcaagactat
    20221 tacattttca ttaatattag cacagacaat ttaactcacg gcttgtaata tgtataattg
    20281 ttacaaaatg atattttgct tgttacctaa tggaggttac atataatgaa aaaaaataaa
    20341 atttctggtt ttcaatgggc aatgacgatc ttcgtctttt tcgttattac aatggcgtta
    20401 tccattatgc ttagagattt ccagtctgtc attggtgtca aacactttat atttgaagtt
    20461 acagatctag caccattaat tgctgcaatc atttgtatac tcgttttcaa atataaaaag
    20521 atccaacttg caggtttaaa attctcaatc agcctgaaag taattgaacg tctactgcta
    20581 gctttaattt tacctttaat tattctaatt attggtatgt acagctttaa tacatttgca
    20641 gatagcttta ttttattaca atcaacaggc ttatcagtac ctattacaca tattctgatt
    20701 ggacatattc taatggcgtt cgtagtagaa ttcggattcc gttcatactt acaaaatatt
    20761 gttgaaacga aaatgaatac gttctttgca agcatagttg ttggtttaat gtactctgtt
    20821 ttctcagcaa atacaactta cggtacagaa tttgcagctt ataatttcct ttacacattc
    20881 tcattctcaa tgattctagg tgagttaatt agagctacaa aaggacgtac gatctacatc
    20941 gcaacaacat tccatgcttc aatgacattt ggccttattt tcttatttag cgaagaaatc
    21001 ggcgatttat tttcaattaa ggtcattgct atttcaacag caattgtagc ggttggctat
    21061 attggtttaa gcttaattat tcgaggtatt gcttatttaa caactagacg aaaccttgaa
    21121 gaacttgagc ctaataatta tttagaccat gtcaatgacg atgaagaaac taatcatact
    21181 gaggctgaaa aatcttcttc aaatattaaa gatgttgaaa aaacaggtgt agctactgca
    21241 tcaacggtcg gtattgctaa aaatgatact gaaaatacat tggctgacga accaagcatt
    21301 catgaaggta ctgaaaaaac agaatctcaa catcacatag ataatctaac tgaatctaat
    21361 catgatgaag atcatgacat cacttcggag tcagtagaat cagcagaatc agttaaacat
    21421 gcaccacaaa gtgacgattt aacaaacgat tcaaatgaag atgaaacaga gcaatcatta
    21481 aaagaacctg cgacttataa agaagacaga cgttcatcag ttgtaatcga tgcagaaaaa
    21541 catatcgaaa aaactgaaga acaatcttca gataaaaaca aataataaca aagcgcttgc
    21601 tagcacctct taagaagatg atgctagcaa gcgcttttct atactatata ttatttttct
    21661 aaaattttaa caccctcttg agtgcctaca ataacttgat ctgccatatc taagaagtat
    21721 cctgtctcaa acacacctgt cagatgaatt aaatactcat ggaacttata tgggtcaatt
    21781 ccttttggta acttacaatc taaaatataa ttgccattat cagttataaa tgcgacatct
    21841 tcgtttacac ggcgttctac ttttatatca gcgtatgatt caattttacg taatatgtga
    21901 taccagttaa atttatccac ctctactggt aacttaaacg tctcacctaa gtattgaacc
    21961 attttcgttt catcgacaac cacaacaaaa cgcgatgcca tttcatctat cactttctct
    22021 ctgaacagcg caccaccgcc acctttaatt atatttaaag atggatctac ttcatcagca
    22081 ccatcaattg ctaagtcgat atgatcaaca tcattgattt cgcatatttt aatacctagt
    22141 tcttttgcta aaaatgcaat tttattagaa gtgcatacac ctgtaatatt gtaaccacgt
    22201 tctttaatta gttgcgccat ttgaggtaag agtaattcca ttgtacttcc tgtaccaatt
    22261 cccagcgtca tgtcaccatt gatttgactt aaaacatcat ttaatgtcat taacttaagt
    22321 gctttgacat ctttcatgaa ggtagcctcc catatttatg taatctattc aattcatatt
    22381 ttacatgact cgtataaatt aacataccct tatttctaac atttttttaa acatatcaat
    22441 cagaatttac aattctaaaa atgttttata gtacaatata ctttaactta acgttaagga
    22501 gaatgatatg atatcaattc acgcaatttc gacaggaaaa atccaagatt tgccttatag
    22561 ctcgaaaaga ccgatgcgct ctgctttaga taaaactaag atttcacaaa caacatggtt
    22621 atcttcaact ggtttcactg gtgatgaaca ggcttataaa gatcatggtg gaccacataa
    22681 agcagtttgt gggtttagta aacataacta tgcactgtat caagatgatt taccttcact
    22741 acctactcat gcgatgtttg gagagaattt aacatttgat tatttagacg aatctgatgt
    22801 ttactttggt aatcaatatc gtttaggtga agcgttaatt gaggtttctg aaattagaga
    22861 accatgctgg aaaattcaag caaaatataa tattcctgat ttagtgaggc gcatgtctac
    22921 atctggtaaa acaggtttct acttccgggt attaaaacaa ggctatgtat ctccaaatga
    22981 tcagctttac ttaatacaag aagcaccaat tgaacatcgt ttatctgtac aacagcttaa
    23041 tgacctttat tataatgata gacaaaatca agatatgtta cgatatgcac taaacaatcc
    23101 atttctgtcg ccaacaagac gcgataaact tcaaaaaatg tataacaaaa cattgaaata
    23161 attacctttc attataagtg ttaaatgaac ttttcaaaac aaaaaggaat caacttacac
    23221 atcgtttgta taatagcctt atctataact actgatgcta ttgattcctt ttttatgtta
    23281 tatattttct ttatttcaaa ctgtatatta atgatgtcat ttcatttgat acgattcttg
    23341 ataacctatt caaaattccg ccaaataaca taaatataat ataaatgccg atacttttat
    23401 tcattttcta ctttttcttc gatacggaaa cttgttttcg aattgaacac ttcaccagct
    23461 tttaaaattg acggtgcttt ttcaccatac aaattaatat catttggtaa aaattgtgtt
    23521 tctaatgtaa agccagaatg tggtttataa atattaaatg gactatccca ctcatcaggc
    23581 tgattaaaag taaagaatac aacatgaggc atatctgtat cgacctctaa cataaattca
    23641 tgattttcaa catacatttt atgttcacca actgtaaatg ggtgatctag accaccaaaa
    23701 cgtgtcattt gttggcttaa ttgcgcatgt tgtgatgtaa aaatatcact caattttatc
    23761 ttgttatctt caaacacttc atgtaaatct agaatatcac ctgtaacaat atttcgctca
    23821 tctaatacat acatatctaa ttgattactt gaaatgcgat gattatcaac gacattatta
    23881 tctcgattca aattgaagta cacatgattc gtaggactaa acaatgtgtc ctctgatgca
    23941 actgcttcgt attcaatcga ccattggtga tccgcatcat aaatatgtgt aatcgtcaaa
    24001 tcgatatcac ccgggaaatg atcatcagct gatttcaaca ccgtcttaaa tatcacttta
    24061 atttgagcaa tttcatttct aatttcataa tcaaataact tattgtccaa accatgacat
    24121 ccaccatgta aatgatgttc gccgttgttt ttttctaact gatattcttt acctttcaac
    24181 ttaaatttag cattatcaat tctaccgcta tatcttccta tagaagcacc aaatttaaaa
    24241 ggattactat gataaaattc atccgcttca acaacatttc caagaacaat attattatca
    24301 tgatatttcc aagacactac tcttgctcca taattcgtaa aaataatttt agtttcatca
    24361 ttatcaattt tgattaaatc tacaccttgt ctttggtgtt caacttcaac tatcattttt
    24421 acttctccct tctaaccaca agtgttcaag ctctgctggg tagcaacatt actaaaacac
    24481 ctacaataca aatgattgca ccgataacat catatttatc tggcatttgt ttatctacga
    24541 ccatcgcaaa aatcaaactc atgatgataa atacgccacc atatgcagca tatactcttc
    24601 cgaatgatgg aaatgattga aatgtcgcaa tgacaccata taacatgagt atcgcaccgc
    24661 ctattagccc aacaagtgaa gactgccctt ccctaagcca cagccaaatc aggtatcccc
    24721 cacctatttc acataagcca gctaatataa atataaaaat cggatataac atgaaatcac
    24781 tccatcacac atttgctacc aataatctat cgtctacata tcatttgttt acatttcttc
    24841 ttacttcaca ttcccatttt aaaaagttcg ttttcacatt catattgtac acttttttag
    24901 acattattct atagctaaat ataaaaaaat aagagtaaca cgctttcatc atcattttat
    24961 atgataaatg tgtgtcactc tcatcaattt tattttttaa atacacgttt cattgaatta
    25021 aataagccac gttcaaatgt aagtactgaa tctttatatg ttttaattgc aatccatatc
    25081 aagacagcta ccattacaat tgagattaat gaacttaaga tgacctcatg tatttgaagc
    25141 cctgaagttt gagcgcgtac aactaattga aatggcgcta aaaacggaat ataacttgtg
    25201 attaaagcaa gtcgcccatc tggattattt atcgtgaata tcgcgatata aaatgcaatc
    25261 ataccaagta atgtcagtgg catcaaagat tgatttaaat cttctattct agatgttaat
    25321 gatccgagaa tggctgcaag taatacatac gccataattc caacaatact acttataatt
    25381 ccgacaataa taatttgcca agacaattga ttcatttcca cgttaaaacc ttgtagcaag
    25441 tcttttaagt caaaggcaaa aatgcatata actgccatca atacaattaa aataatctga
    25501 gtcacggcaa ctgctataac gcctaaaatt ttagcaaaaa tatgttggat tggtgaaata
    25561 cttgtaataa tcatttcaat gacacgcgat gttttctcac tagcaatttc catagctatt
    25621 tgagatgcat aatttaaaac aataaagaac attagaaaga taatgccata agctaaagca
    25681 tagttaaaaa tcttttgtcc ttctgatact ttatcgactt catcattaga aatcacctta
    25741 ttatcaactt tactttgtgc ttgtaatttt tgtaagtctt ctttgttgat atttaattcc
    25801 ccggctacca tatttgtttg aatagctgta agcaatgctt gtactttttg tgaatcttca
    25861 tgacttactc gcttctcact aatgattgtc ccttgtaacg tgcgattttg attcaacttg
    25921 ataatataag ctttatcaag tttatgtttt tttacttctt tttcagcatc ttctatagaa
    25981 actttagtaa acttagcatc actatgaaat gtatttgcct gttgcttgaa aaccttatag
    26041 atttgttcat tcggtgctgc tacaccaatt ttatctggac catcatcaaa catgttaata
    26101 atcttatcaa tgttagatag gccaatcatt aaggcagcaa taataatcat aaaaattaca
    26161 aatgatttag ctttaatttt tttgatatat gtcaaagtaa atgtcgccca aaacttatgc
    26221 atccttgcca ccaaccttct caatgaatat atcttgtaat gatggttcta caacttggaa
    26281 acgtttaaca taaccttgat gtgccacaac ttgataaata tctttggcta cgtcttcatt
    26341 ctcaatcgtc aactgaagac cttgcttcat gttttcacta tgaatgatgc ctctaatgtt
    26401 tgttaaatct ggtagtgttg tttctgattc aatgacaact ttcttgttac cattagatgc
    26461 acgtacatga ttgatatcac cagaaacaac aagttgacct ttatctaaaa tacaaacatc
    26521 atcacataat tcttcaacat gctccatacg gtgagaacta taaacgattg tactgcccca
    26581 atcatttaag tctttaactg cttcttttaa taactcaaca ttaactgggt ctagaccact
    26641 gaaaggctca tctaatatta gtaattctgg tttatgtaac atacttgcta acagctgaat
    26701 tttttgttga ttcccttttg atagactatc aattcgtttt ttgcggtttt cagtaatatc
    26761 aaaacgctca agccaatacg atatttgctg ttgtatttct atttttgaca ttccctttaa
    26821 agttgccaaa tacttcaatt cttcttcaac tgtcaatttc ccatgtaaac cgcgttcttc
    26881 cggtaaataa ccaatacgat tgtacattgt tttatctagt tttttaccgt tatacgtaat
    26941 gtgtccttca gttggttcac ttaagcctaa aatcatacga aatgtcgttg ttttacctgc
    27001 accatttctt cctagaaaac ctaacatttt gcctgattct aactctaatg acatatcatt
    27061 tactgccgtc atcttgccaa aacgtttcgt aacatgttca attacaagtc ccatacttcg
    27121 cctcctaaaa caatatgtat ttatcttaat ataacatttc tattctctat aaatgcaata
    27181 tttttaaagt gaatttattt ttaaaatttc tgaaattgaa atatttaaat agtatcttct
    27241 ttgcatgtta agtatgatta gtgttagata cgcattttcc atgcctttat tgccttattg
    27301 gtaaaaaaat atcccagcat cattgtgccg agatatttat atccttacaa ttgattatct
    27361 gtttcttccg gtgttttcgg tgtttcagat gtacccgctt tttccggtgt ttcagatgtc
    27421 actggttgat ttttaattga agatgggtat tttgggcttt cgccatattt atttgccggc
    27481 ttactgtcaa agcataggaa tactatcata acaattgtta ggcctaaata aacaaaataa
    27541 agaagtacta acaaaatatt aagacccatc ggcattaagg taaaatcact gtcataataa
    27601 ctatcgataa tctgtaatac tatataaaat ataatactga atactgtcat aatcattgga
    27661 aataacattg ttcttgatat atcgtgaaat cttcgaacgc acaacgctaa atttggaata
    27721 aacgttgcca aactatagac aaaagtatac acagatgtaa ggataatcat caatatactc
    27781 ataactatta atgcttcgtt atctgccgct atagaaataa agaatagaaa taggtttatt
    27841 attagcacac acacagctgg aaccataagt atcaaatgcc atagtgccat ataccaatat
    27901 tcactacgtc ttgatctccc cttaaaatta acataatttt tccaaaataa tacgaatgat
    27961 ttcataaaac ctacttgagg taattgttcc attgtaatct ccctttcatt tatcatattt
    28021 atatttttaa ttattattac cgttataatt tacaagattc attatcaagc cacttcattt
    28081 caatcgtcat tcactttgga aaatgaattt tatatttcat cactagtaaa atgaaaaccc
    28141 gctacaagta cgcatctata tagagactca tttgaaagtc aacgcttcgt taactatact
    28201 aaaaatatgt cacactgtaa tgttcacgtt aagaagagtc tcaatctatg caaataaaat
    28261 attccataac aaagtatata ctttacattt ttataattct taacaatact attttaacaa
    28321 acatttacca caataaaaat atctttttca tttttattta aattaatcat ataattacga
    28381 ggagaatatt atggatttcg ttaataatga tacaagacaa attgctaaaa acttattagg
    28441 tgtcaaagtg atttaccagg ataccactca aacgtataca ggctacatcg tggaaacgga
    28501 agcatactta ggtttgaatg atcgtgcggc tcatggctat ggtggtaaaa taacacctaa
    28561 agttacgtca ttatataaac gtggtggtac aatttatgca catgtcatgc atacgcattt
    28621 actcattaat tttgtaacaa aatctgaagg tatacctgaa ggcgtactta tccgcgcaat
    28681 tgaaccagaa gacggtttat ccgctatgtt ccgtaacaga ggtaagaaag gctacgaggt
    28741 aacgaatggc ccaggaaaat ggactaaggc atttaacatt ccacgagcta tcgatggcgc
    28801 aacgttaaat gactgtagat tgtctattga tactaagaat cgtaaatatc ctaaagatat
    28861 tattgctagt ccacgaatcg gtattccaaa taaaggtgat tggacacata aatctttacg
    28921 ttacacagtg aaaggtaatc catttgtgtc tcgcatgcgt aaatcagatt gtatgtttcc
    28981 cgaagatact tggaaataaa tgccatcttt cattgattac tatcatgaaa atgaaatcta
    29041 tctccttata agtcaatcaa tcgtgccgtc aacatgcgga tgggttgatt gtttttcttt
    29101 gtgtccatca tattttttga ttcatctcct cttattgaac ctgttcttaa ttataaaata
    29161 taacaataga attatttata attattaaat ttagatgcat taatattatt gatattattt
    29221 tcaaaaacta gaaatattga tttattgcat gtataatgtt aaaagtgccc ttttataacg
    29281 cttacatatg aaagcttatt tagggagagg gatattcaac aagggggatt tgaaaatgat
    29341 agaacttaat gcaattacaa cattatgttt agcctgtata ctttacttac ttggtaaagc
    29401 tatcgttaat cacgttaatt ttttaaaacg catttgtata ccagcaccag tgattggtgg
    29461 cttaatcttt gctattttag ttgcggcttt ggattcattt ggcatggtta agattaaact
    29521 agatgcttca ttcattcaag atttcttcat gttagcattc tttacgacaa tcggtcttgg
    29581 tgcatcattg aaattattta aattaggtgg caaagtcttg ttattatact ttatgttttg
    29641 tgctatcatt tcaatcattc aaaacatagt tggtgtatca ctagcaaaag tattaaatat
    29701 taaaccgtta ctaggattaa cagcaggttc catgtctatg gaaggtggtc acggtaatgc
    29761 tgccgcttat ggtaagacaa tccaagattt aggcattgac tcagcattga cagcggctct
    29821 tgcggctgca actttaggtc ttgtatttgg agggcttatc ggtggaccag ttgtaaaatt
    29881 tttaatcaaa cgttataact taaagcctca acatagtgac gatacattta aagattatag
    29941 ccaagtagca tataacgaac atttacatag taaatttaat gccactgaag tattcttcat
    30001 tcaatttaca atcgttgtat tctgtatggc agttggaagt tatttcagtc atttgtttac
    30061 agctcaaaca gggattaatg ttccaattta cgttggttca ttatttgtag ctgttattgt
    30121 ccgaaatatc tccgaaagtt ttaattttaa tattgtagat ttaaaaatta ctaatcaaat
    30181 tggcgatgtc gcattaggta ttttcttatc tcttgcgcta atgagcattc aattaatcga
    30241 aatttataaa cttgctatac ctcttattat tatcgtttta gttcaagttg tcgttatgat
    30301 tttatttgct gttttaattt tatttagagg tttaggaaaa gattatgatg ctgcagtaat
    30361 ggtaggtggt tttatcgggc atggtcttgg tgcaacgcca aatgccatgg caaatttaga
    30421 tgttattact aaaaaatatg gaaactcacc taaagcatat ttagttgtac ctattgttgg
    30481 tgcattctta atcgatttaa ttggtgttat agtcattatg ggattcattc aatggtttag
    30541 ttaaacacca aactcataaa taaaagagga ggccttcgcc tcctctttta tttatcctcg
    30601 atgtatattc aagctacgtt gttctatcca tgacaatatt tccggactaa atacgatttg
    30661 tttttgtgtt aagtcgtcaa tatttttagc atctaacatc gtcattattg atttcatgtg
    30721 ttcaataaat gattctacat aagctactgt atgtgcaatg ccattatttt caacttgatt
    30781 taaaaacgga cgtgacatac cagttgcctt tgcaccaagt gctaaacttt taattgcatc
    30841 gagtggtgta cgtaaaccac cactcgcgaa aactgaaatt tcgctttgat aagccgttgt
    30901 ttcaagtaat gactcaactg tagactgtcc ccatgatgat aagtaatcca tatctttatt
    30961 tgcacgacgt tcattttcaa tatctacaaa gttagtacca cctttgccac taacatcgac
    31021 atacttaacg cctatttgtt gtaagtcatg cattaattct ttgctcatac caaatccaac
    31081 ttcttttata atgactggaa cagatactcg tgatacaatc gacgctatat tatctaacca
    31141 agtcacaaat tcacgattcc cttcaggcat aactaattct tgaggagaat taacatggat
    31201 ttgtaacgct tgtgcctcaa gtaattcaac tgcttccaaa gccttttcta ctggtacgtc
    31261 cgcaccaaca ttgctaaaaa tcatgccttc aggattcatt tttcgcgcaa tcgtaaatgt
    31321 ctcagccatg cgtggatttc tcaatgctgc atgtgttgat ccaactgcca tcgctaagcc
    31381 agtttctctt gcaactacag ctagcttttc attgatgttt ttcgtccact cgctaccacc
    31441 cgtcattgca ttaatataaa ccggatatgt catcgttaag tcaggtgtct gtgatgtcaa
    31501 atcgatatca tttacattaa ttgatgggat agaatgatgc acaaaacgca tcttatcaaa
    31561 atctgaatgc attgcgtcag attgggccat tgctatttca acatgttcat tttttctctg
    31621 ttctctttga aaatcactca tgattaaacc taccttttcg tcatttctaa tttaaaacca
    31681 ttttttacgt ctgaaaaaga tgattaatac gacagctata acaaacatta cagctaagca
    31741 aatataatat ccataatgta aatgtaatgc cggcatattt acaaagttca taccataaat
    31801 cccagctatg aatgttaacg gtgaaaatat aactgatact aatgttaata cttgcataat
    31861 actattcatt ctaaatgacg tgtatgactc aaaattttct cgtatttcat ttgtcatttc
    31921 ttgtgcagta cgaatgatat tacgttgctt aatcaagtgg tcatcgatat gttgaatgta
    31981 tagcgaatgt ttattatcta taatcaaatc accattttgt ttcattgtat caattagctc
    32041 ctgcatagga aacagtacac gttttacttt aatcaaatcc gaacgtaact taaagacact
    32101 atccatgacc attttattaa agcgatcatc tacatggcgg tcttcaaaat gataaacact
    32161 atcttcaagt gcatatacaa agttgaaata tttatcaacc atcatatcta aaattaatat
    32221 gacgacatct gcacaatcta attctgcatc taatgtattc atatacttat agactacttt
    32281 atttaatgat tccaacgttt gatgatgata tgttactaat acattgtctt gtataaaaat
    32341 atttagtgct attggtgaat agtttgaccc cataatacta tggaatacta agtattgata
    32401 atctttataa gatttatatt tagctcgtgg cataccgtta attgcatcat ccacttctaa
    32461 atcattaaaa ctaaaatgtg ctttaaacca ttcattttct tgttcattcg gttcatcaaa
    32521 atcataccaa acaatagtcg catcttttgg tatctcttta atatcatcaa ctactttaaa
    32581 cggttcgtat gtagtttgat accgtatcgt taaagccatc gatactcccc ctaaataacg
    32641 aattctctat tattttatca tgaattaaat aacgtgtatg tcttaattta ttttagtatg
    32701 atagtcacta aggagatggt tattatcaca caacttttta cacatactca aaccgtaaca
    32761 tctgaattca tcgaccataa caatcatatg catgatgcaa attataatat catttttagt
    32821 gacgtcgtga atcgttttaa ttacaaccac ggtctttctt taaaagaacg cgaaaattta
    32881 gcatatacgc tatttacact agaagaacat acgacatacc tctcagaatt gtctcttggc
    32941 gatgtattta ctgttacatt atatatttat gactacgatt ataagcgatt gcatttattt
    33001 ttaacattaa ctaaagaaga tggtacacta gcatcaacaa atgaagttat gatgatggga
    33061 attaatcagc acacacgtcg ttctgatgct tttcctgaat cattttcaac acaaatagca
    33121 cactattata aaaatcaacc aactatcact tggcctgaac aattaggaca taaaatagca
    33181 attccacaca aaggagcatt aaaatgacag atgcattaca acaaaagatt catatcgaat
    33241 tactagattt attagatgat gttaagtttg aattaacaga attaaatgca caaaaagggt
    33301 tatacattaa cggaccagca aatcagttac ttaagcgtgg cgtgcatatg gcttatgttc
    33361 aaggacaaaa gcaagctatc gataatatta tgactattgt ggaacaacag cttgaagatc
    33421 aacatttcct agaacattat gataaatttc aaaatgaagt tgctcatcgc aattatgata
    33481 aaacagctaa ctttgcagaa cttagtgata ttccacgtca attcgataat tttttagatc
    33541 aattttatca aattaaaggg caatacttta tcatcacaca tatcaataca cttattggtg
    33601 attttcactc agaagctcat taacaattag tctatataat ccttgctata ttttcaaaaa
    33661 caaaacccag ttgcgttttc atgttaaata tcatcttcca tgaaatcgta actgggtcaa
    33721 ttgtacgtta ttagttattt tgtgttacat cctcatctat cgatttggca atttgtttaa
    33781 tagctttatg tgattgtcta attggataaa ttggaaaatc atgtaccatc ttaggataat
    33841 cataaaactc aatgtattga tgatgttgca acatcatttg ttcgaacaac ttcatatcag
    33901 gatgtgtcat ttcacgtcca ccaccaaaca tataaactgg tggcaatcct tctattgtgc
    33961 cattaattgg cgatatgcgc ttatctgtta atggtaggcc attcgcccat tttttcataa
    34021 tctcattgac accaaactga cttagaaccg catcttgttc gattaaagcg tccgaaatat
    34081 ctttattaga tagtgttgca tctaaaattg gtgatattaa atacaattta ttcggtaatg
    34141 gctgttgatt gtctaaaaga aattgtacaa aggataatgc cagtgcacca cctgaaccat
    34201 cacccatgac tacgacattt tgatggccta cttcagatac taattgatca taaacacgtt
    34261 gtatcgcttt gaaagtgtcg tcaatatgaa actctggtgt ctttggatag ataggcagta
    34321 caacctcata taatgtactt aaagtgattt tatccaacaa tctccaatgg aacggtgatg
    34381 gttgtagtgc attgaatcca ccgtgaatat ataaaatttt cttatcaatt tgatgtctga
    34441 aattaaagcg aaagacttgc atatcatcta atgataattt ttctaaattt gctttaacat
    34501 ttaatgttga aggctgctta tgtttttttc tattttcaat ttctctttta taaaaaaatc
    34561 tttcaacatc ttgatcattt ttaaacataa tcgagcgatt gtgaagcaaa tatttattga
    34621 caacgctatt cataacacgg tttctaatca atgtcttaac ctacctt
//
LOCUS       DAPIDM010000012        33846 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000012 DAPIDM010000000
VERSION     DAPIDM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.012"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..525)
                     /gene="srtA"
                     /locus_tag="RJE42_000518"
     CDS             complement(<1..525)
                     /gene="srtA"
                     /locus_tag="RJE42_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDV5875971.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGK"
     gene            719..1210
                     /locus_tag="RJE42_000519"
     CDS             719..1210
                     /locus_tag="RJE42_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV5875972.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERIAWFET
                     KQRNHEPIFVFEENGSVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQH
                     LIVEAKAKGYRTLVAGIDASNEASIKLHQKFNFKHAGTLTNVGYKFDYWLDLAFYELD
                     LKD"
     gene            complement(1354..1524)
                     /locus_tag="RJE42_000520"
     CDS             complement(1354..1524)
                     /locus_tag="RJE42_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875973.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(1586..1684)
                     /locus_tag="RJE42_000521"
     CDS             complement(1586..1684)
                     /locus_tag="RJE42_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875974.1"
                     /translation="MIKKKHLSGLLVPDRCFLIIYDEVEKFMVNSF"
     gene            complement(1703..2602)
                     /gene="sdaAA"
                     /locus_tag="RJE42_000522"
     CDS             complement(1703..2602)
                     /gene="sdaAA"
                     /locus_tag="RJE42_000522"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDV5875975.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(2616..3296)
                     /gene="sdaAB"
                     /locus_tag="RJE42_000523"
     CDS             complement(2616..3296)
                     /gene="sdaAB"
                     /locus_tag="RJE42_000523"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDV5875976.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(3299..4342)
                     /locus_tag="RJE42_000524"
     CDS             complement(3299..4342)
                     /locus_tag="RJE42_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV5875977.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            4891..5094
                     /locus_tag="RJE42_000525"
     CDS             4891..5094
                     /locus_tag="RJE42_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875978.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            5277..6212
                     /locus_tag="RJE42_000526"
     CDS             5277..6212
                     /locus_tag="RJE42_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV5875979.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGIFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPESLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(6294..7262)
                     /locus_tag="RJE42_000527"
     CDS             complement(6294..7262)
                     /locus_tag="RJE42_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5875980.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPA
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(7395..7739)
                     /locus_tag="RJE42_000528"
     CDS             complement(7395..7739)
                     /locus_tag="RJE42_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV5875981.1"
                     /translation="MVKQLNSVVAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(7780..8190)
                     /locus_tag="RJE42_000529"
     CDS             complement(7780..8190)
                     /locus_tag="RJE42_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV5875982.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(8564..10630)
                     /locus_tag="RJE42_000530"
     CDS             complement(8564..10630)
                     /locus_tag="RJE42_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDV5875983.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKKLVIEDVQDADPGK
                     LIMTVK"
     gene            complement(10930..12669)
                     /locus_tag="RJE42_000531"
     CDS             complement(10930..12669)
                     /locus_tag="RJE42_000531"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDV5875984.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMTKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(12709..13398)
                     /locus_tag="RJE42_000532"
     CDS             complement(12709..13398)
                     /locus_tag="RJE42_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDV5875985.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGKFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLIILFY"
     gene            complement(13391..13786)
                     /gene="cidA"
                     /locus_tag="RJE42_000533"
     CDS             complement(13391..13786)
                     /gene="cidA"
                     /locus_tag="RJE42_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDV5875986.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKQRKGIDAYE"
     gene            complement(13999..14877)
                     /locus_tag="RJE42_000534"
     CDS             complement(13999..14877)
                     /locus_tag="RJE42_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5875987.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            15044..15262
                     /locus_tag="RJE42_000535"
     CDS             15044..15262
                     /locus_tag="RJE42_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDV5875988.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            15650..16081
                     /locus_tag="RJE42_000536"
     CDS             15650..16081
                     /locus_tag="RJE42_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5875989.1"
                     /translation="MKYKKILFRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(16221..17501)
                     /locus_tag="RJE42_000537"
     CDS             complement(16221..17501)
                     /locus_tag="RJE42_000537"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDV5875990.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEEQFNILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWGYDQERQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQDLRSQQ"
     gene            17756..18922
                     /locus_tag="RJE42_000538"
     CDS             17756..18922
                     /locus_tag="RJE42_000538"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDV5875991.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(19093..19614)
                     /locus_tag="RJE42_000539"
     CDS             complement(19093..19614)
                     /locus_tag="RJE42_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV5875992.1"
                     /translation="MDYKCYYDSPVGRLELVSDGVSLTAVLFENQQDDGTREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            20023..22131
                     /gene="clpL"
                     /locus_tag="RJE42_000540"
     CDS             20023..22131
                     /gene="clpL"
                     /locus_tag="RJE42_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDV5875993.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISI
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDTEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDLDIEDNELVVKGKS"
     gene            complement(22198..22368)
                     /locus_tag="RJE42_000541"
     CDS             complement(22198..22368)
                     /locus_tag="RJE42_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDV5875994.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDIDRDCCG
                     TEQHTANHFPGK"
     gene            complement(22381..24375)
                     /gene="feoB"
                     /locus_tag="RJE42_000542"
     CDS             complement(22381..24375)
                     /gene="feoB"
                     /locus_tag="RJE42_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDV5875995.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYDEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFGGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYIGPHGINVNINQSFLHIVGSFFGMLVQPLGFGTWQA
                     GATLGPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(24387..24614)
                     /locus_tag="RJE42_000543"
     CDS             complement(24387..24614)
                     /locus_tag="RJE42_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDV5875996.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(24830..27298)
                     /gene="farE"
                     /locus_tag="RJE42_000544"
     CDS             complement(24830..27298)
                     /gene="farE"
                     /locus_tag="RJE42_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDV5875997.1"
                     /translation="MGTFIAKHKWSTLIAWVIIVAAILIPLATNAPKFDNDIKMTGLE
                     SLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGILQPSITKDIKKTFDDIKKDDKHIDK
                     ISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKNHLKDLRDNHNVQTELTGT
                     GMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGIISLLTYVF
                     DIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDFVKGIGLAVGTAGSAVIFAG
                     VTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSTLTLLPALISIFHKRIKVNKLQS
                     NFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPADSTQKKA
                     YDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDIVTPPQMS
                     KDNDYALMVVIPKHGPNAESTNDLVHDLRDYNKDAQDKYGFKTEISGQSVINIDMSKK
                     LNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTFVMQDGFMK
                     GLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYSIKVGLKE
                     SGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIPALTKLFG
                     KGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVYPQGATNV
                     SKNQDVHGQDDVRSIVLDDKTMALYQEVKQQSASSLFLYDALMDYQNKHQLNSKQQAT
                     NIEQLNKNIEKLNQLLEKNLRNKS"
     gene            27464..28012
                     /gene="farR"
                     /locus_tag="RJE42_000545"
     CDS             27464..28012
                     /gene="farR"
                     /locus_tag="RJE42_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDV5875998.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNLICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLRDIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEEIDKIFHKVINIKIK"
     gene            complement(28063..28257)
                     /locus_tag="RJE42_000546"
     CDS             complement(28063..28257)
                     /locus_tag="RJE42_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5875999.1"
                     /translation="MRYVISIIMGIALAIWSFKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKTIVVHEFINV"
     gene            complement(28369..29913)
                     /gene="pruA"
                     /locus_tag="RJE42_000547"
     CDS             complement(28369..29913)
                     /gene="pruA"
                     /locus_tag="RJE42_000547"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDV5876000.1"
                     /translation="MVVEFKNEPGYDFSVQENVNMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(30103..30702)
                     /locus_tag="RJE42_000548"
     CDS             complement(30103..30702)
                     /locus_tag="RJE42_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDV5876001.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDKN
                     KRKELIDELFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPINIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNEVPSEALNDETLN"
     gene            30941..31132
                     /gene="cwrA"
                     /locus_tag="RJE42_000549"
     CDS             30941..31132
                     /gene="cwrA"
                     /locus_tag="RJE42_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDV5876002.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            31372..33780
                     /locus_tag="RJE42_000550"
     CDS             31372..33780
                     /locus_tag="RJE42_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDV5876003.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKNGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDDQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT        10802 a         6408 c         5238 g        11398 t
ORIGIN      
        1 tttacctttt tgttcatcta gaacttccac atctgttggc ttaacatctc ttatacttgt
       61 cattttatac ttacgtgttt cattaccaac tttaaagtac accatactac cttttttggc
      121 tgctttaaga tttgtaaatt gatagttcgg acggtcaatg aaagtgtgtc ctgcaattga
      181 aatattttga tcatctagtg attcattttc ttctgcaaag cttacacctc tatttaattg
      241 ttcaggtgtt gctggtcctg gatatactgg ttctttaata tcagcatctg gaatttcaat
      301 atagcctgcc acttttgatt tatctttcgg aatctgaggt ttagcttgct gcttattgtc
      361 tttactcgcc tgttctttta catttttatc atattgttca atcttttcat ctttatcttt
      421 atcgtgaaga taattatcga tatgtggttt agcaaacaaa tatgctgcca ctaggataag
      481 tactacacca gcgattgtca ttaatcgatt tgtccatttt ttcatacgtt aaggctcctt
      541 ttatacattt cacaatgcct attaacattt catatagtat accattaatt ataaaatgac
      601 tcatagcaat tcattttata atattaaatt tgcacatata ttgtcacgtt agatttcatt
      661 acacatattt gcattcaaat aacgaaacgt ttttaataca tattaagggg gaattattat
      721 gattcgatgt gctaaaaaag aagatttaaa cgctatatta gcgatataca acgatgccat
      781 tattaataca acagctgttt atacttatga accgcaaacc atagacgaac gtatcgcatg
      841 gtttgaaacg aaacaacgta atcatgagcc tatctttgta tttgaggaaa atggaagtgt
      901 attagggttt gcgacgttcg gttcgtttag accttggcca gcatatcaat atactatcga
      961 gcattctatt tatgtcgatg cttcagctag aggaaaaggt attgctagtc aattattaca
     1021 acatttaatt gtagaggcaa aagctaaagg ttatcgtaca cttgttgctg gtattgatgc
     1081 ttctaatgaa gcgagtatta aattacatca aaaattcaat ttcaagcatg ccggcacact
     1141 gaccaatgtg ggctataaat ttgattactg gttagattta gctttttatg aattagattt
     1201 aaaagactag taaggtttga atcacataat ataaataaga caaccatgtt aattcactta
     1261 acatgacaac atataaaatt ataaacttct cagggacaat ttacatcgtc ccttttattt
     1321 tttcatttca aatactatcc ccatagcttt gacttaacgc tttttctcaa taacaaaacg
     1381 aatatagtaa aacatgaaaa cgataatcat gctgagtgat aaagatttaa ataatagatt
     1441 gacccacgtt ccctcagtcg tatatccata tgtaatcgtt gtgttaatga tgaatgctat
     1501 aaagatgatt gatagtctta gcatatcatc actcctttta agttatttta gatatacggg
     1561 ggcgcttttg caatcactat tttgattaga atgaatttac cataaatttt tcaacttcat
     1621 catagataat taagaagcat ctatctggta ctaataatcc agacagatgc ttctttttta
     1681 tcaatatctt attgttatct cattaattat ttttaaccat atcttcagct gtgccaaaga
     1741 ttttacgttt aattgcttcg ccagttggtg tgcctgctag cccacccaat ccagtttcac
     1801 gtaatgatgc aggaaggtta cgaccaacct tatccattgc ttcaataact tcatcaacag
     1861 ggattctact ttcaatacct gctaatgcta aatctgctga aattaaagcg ttacccgaac
     1921 caattgcatt tctcataaca caaggaattt caacaagtcc ggctactggg tcacaaacta
     1981 aacctaataa attacttatc gctaatgcca tagcgtgccc ggatgcttct ggtgatcctc
     2041 cgaaaatagc tactgctgca gctgccgcca ttgcagatgc tgaaccaact tcagcttggc
     2101 agccacctgt tgcaccagct acacttgcat tgtttgctac gacacgccca aacaatgctg
     2161 aagtgaataa gaaatcaatc atttgctctt ctgttaaatc atgtgttttt tctaatttaa
     2221 aaagtgcacc gggaatggta cccgaggaac cagctgttgg cgttgcacaa ataataccca
     2281 tcgcagcatt gacttcattt gttgcaatgg cacctttgac tgcatcaatc atttcatatc
     2341 cagacaaagc atgatgtgtt tcattataat cacgtagttt agcagcgtca tgaccagtgt
     2401 agcccgttac actttcaacg ccatcacctg tcgtcccttt gattactgca tctcgcatga
     2461 catctaaatt ttgtttcatt tgcgctcgca cttcatcacg tgatttaccg cttaattcca
     2521 tttcttcttt aaccatgata tccgcaaatg acatattatt ttctacggca taatctatag
     2581 tctctctaat tgaatcaaac atgtttattc cccctctaat ttatatagga aacgtttacg
     2641 tcactgtatt tctctttaat tgtatttaat gtcgattctg agattgcttt atttaatggt
     2701 attacaacca agcatttatc ttcatctatc ttaataaatt catctttaca gtctaatttc
     2761 atatcgttga tatcattaat gaaatgattt acttgtgctt tagtcatatt tccgtcaaca
     2821 actaaaattg gtaatccatg atttaaatct acttctagtc catttatatg aataccttta
     2881 attttaattg taccaccacc gattgaaata ccgataattt caatgtagcg accatcatta
     2941 cgagatgatt taatataagc acagtttgga tgttgaccaa tactatcgcc ttcttcttcg
     3001 atgatatcta ttttaatacc atcatcagct gcaatttcta atgaagattt aattcggtta
     3061 tcaaatgttg aatatcccat tgctccaccc acaatagcga catctgtacc atgtccttgg
     3121 tgtgtttgag caaatgattc ataataatgt atttcaatat ttttaggaat atctcccaat
     3181 attgcgcgtg ctgaattccc aatctttact gcaccagccg tatgagaact tgaaggtccc
     3241 atcataactg gtccgataat atcgaaagca ctttgataat catagctctt tgccataatt
     3301 aaacactctc cttaatatga ttctttttgc tcggcatttt aaagttgata ttcattaaat
     3361 taaacttatt aattagtgtt ccaataatat aggctaagac aatgctgaca ataatcactg
     3421 ttgctattgt tacaattgat gtcactgcat tattaaagcc aaacaatacg atggcacctg
     3481 caattggtgt tgccatacct ttaacaccta ttactagtcc gctaaatgtc acgatacatg
     3541 cgttgacgac cccaatcagt gcatttgtac catatagttg tactggatat tgcgctatta
     3601 aatcaatttg cgtcaatggc tcaatacaaa ctgcaaatgc ttttgacggt ccaccaatgt
     3661 ttaattttcg gaataaaata aggttaacaa atgagctacc tgtacatgtt agtgctccaa
     3721 tagccatagg aacacctgtc agtcctaata aacttgttaa taccattgaa cttagcggtg
     3781 tcatacctgt aacaggaatc actagtccta aaatgaccgc taatgcatat ggattgttat
     3841 cacctaccgc agtgacagca ctacctattt gttttagtgt tgctagcaca ccaggtgtaa
     3901 tgattgatgc aagtccgaaa gcaattgctg gtgcaaataa aatcaccaca attaagtcca
     3961 agccttctgg aactttcttt tcaatccatt taattaaaaa agctacgcca taagctgcga
     4021 tgaatgctgg taataattta aagtcatgta atactaaacc aacaatgacc gcaaatactg
     4081 gtgcaacgcc taagtttaag cacgttagaa tacctactgc gataccgctt aaacttcctg
     4141 ctaaatcccc aatatcttgt agaaatttaa tatcaaatac gccaccaata gcgtaactta
     4201 agaatgcttg tggtaaaaat gtcgcacaag ctgcaccgga taatgcttgt agtccttgtt
     4261 taccgtacgg tgcatacttt aaaaatagcg tcatgatcac taaaaccaag actaatgtgc
     4321 ctacacctaa cagaatatcc atttcccaaa aacctctctc tatgtttatt ttattttcag
     4381 accataaaca tcgtacaccc ttaagaaaac gttttcaact tttatctgtt atcaaatcaa
     4441 atatttaagt gaaatatttc atatttgtga aagattttta aaatggattg tttcaaaaac
     4501 tacttatgtt gtcattaata tttactaatt aactttttac tctgtatttc aaacagttgt
     4561 gcgacagttt tttgataact tttttacatc cgaaaataag taatatttct aaaaactttt
     4621 aatatttata cattttatct ttcgagctat ttatttttgt ttttgtgctg tgttgataaa
     4681 accgcacact atattcaaaa ataacttatt acacatatat ttgcaataag ataattaaag
     4741 taggatatta tttttagttt tctgatagga atgatgatag tctataggtt gaatctttac
     4801 tttttttaaa gctaaattta catcaactta acaatgtctg gttatactga agatgaaaat
     4861 atttagtata acttagtgga ggcggtaaag atgcaattga gtcattccgt taaagttgca
     4921 atctctatct atttagcact tatctttata acgttcactt cttatttagt cattatttta
     4981 tatacgagta tgactggaca tgatgtatca catttcgtgt tagatagtca gcattctcat
     5041 catggatctc ttacgcaaaa acatttgagt cttcctgaaa tctcatttaa atagtttatc
     5101 ctctatgttt caacattcat ttcccatatc gattcattta tctatcatct agaccactac
     5161 atcttagatg atttttttat tttttcattt cactctttct taaaagtcga tataatgaat
     5221 taaatcatta tcatacaccg acatatttta tgttgttggt gttaagtttt aaaggggtga
     5281 gatacttggc gaataatcat tcagctttgc gtttaaaagg aattatactt gccattgtcg
     5341 gtgcttgttt atggggatta ggtggtactg tttctgattt cttgttcaaa tataagaata
     5401 ttaatgtcga ttggtacgtc actgctcgac ttgtagtcag tggtattttc ttacttatta
     5461 tgtacaaaat gatgcaacca aaacgttcta tatttagcgt attccaagat cgacgtatgt
     5521 taggcaaatt acttatcttc agtatactgg gcatgttagt tgtacaatat gcttatatgg
     5581 catctattaa tacaggtaat gctgcgattg caacattact acaatacatt gcgccagttt
     5641 atattattat ttggtttgtc ataagaggcg ttgcaaaact aacattattt gatgtgcttg
     5701 ctattatcat gacactatta ggaacatttt tattattaac gaatggttca ttttctaatt
     5761 tagtcgtcaa tcctgaaagt ttattctggg gtattttagc cggtgtagca ctcgcttttt
     5821 acacaattta tccttcagac ctacttaacc gcttcggttc gattctaatt gtcgggtggg
     5881 caatgcttat ttctggtgtt gcgatgaatt tacgccatcc aatttggcac attgatatca
     5941 ctaaatggga catatcaatt atattatttt taatctttgg tattatcggt ggtaccgcac
     6001 tcgcatttta tttctttatc gacagtttac aatacatatc agcgaaagaa acaactttat
     6061 tcggaactgt tgaacctgtc gtagccgtta tcgcaagcag tctatggtta catgtggcat
     6121 tcaaaccatt tcaaatcgta ggcatcattc ttattatgat tttaatttta ttactgtcac
     6181 ttaaaagaca acctgaaaca ttagatgaat aagaaaactc tgataatcac tttagcaagt
     6241 aactattatt taacaatgta gctaccttgt aggtgatatc agagtttttt attttagtta
     6301 ataatatttt tcacttggta taaaaagcgt cggcgctctg gtatcggaaa tactggataa
     6361 atatggaatt ggtaatatcc aggtataaag tcatgttcga tacctttcgc actcaacaat
     6421 tgcgataagt tcaaagcatc tgggtacagt acttcttttg tgccaacagt taatgtaata
     6481 cgacctaaac catctaagtc accattaatt ggtgatactt tgtagttatc taaaggtgtg
     6541 tcccctgccc attgttcagc taagaacaca ctgccatcca cacctaccat tgggtctttc
     6601 tttaagtagg caggaatttc aggatgctgc atcgttgcat ctaaaactgg tgaaattaat
     6661 acaatatgtc ctggttgcac aatatgtttt tcttttaaca attgagcaaa tgataaagca
     6721 atttgaccgc ccgcagagtc acccattaca acgatttgtt tagaatctgc tacttgattc
     6781 aataaatcat ggtataactt ttcaaaaagc acatacgtcg cttgataatc ttgatgcgga
     6841 atcttaggat aaactggcat gatgacttta gcattgagtg tttctgcaag ttcatcaata
     6901 aattcgaaat gaattttgag tgggtcttgg aaccatgcgc ctccatgtgc gtataataca
     6961 acgcgttgat gcttatcttg tttatcgtta acggtataaa cggttgaacc gaagtgctta
     7021 tccactttta ctggcatctt aaactgataa ttggtgccct catattcacc tgctgtctgt
     7081 ggcgccattt tttctaatgc tttcatcgct tcttctttat acataaaacg acgcttagct
     7141 ctagataatc gtataccttg ttctaacata taacttttca cactgcgttt ttctttaatt
     7201 ctaatatgtg cgtatgccgc tactaaaaat ccaaacgcaa gtgtagacca ttttttacgc
     7261 atttgtcgtc actcctcttt gatgatttct actatagttc tactgtacca tgctgattct
     7321 tattactacc aatatttact gggaaataag ttatgatgtc tacataatct tagcaatgct
     7381 aatcatttgc gctattaatg ctcatcgaca tcgttattca cactatcatt catttgctta
     7441 agatgatgtt caaaacgttg catatcgata aaacgccctt gtcgatgcat ttcttttcca
     7501 ttcataaaaa ttaaatccac aggtacagtg aaaatattta attctccagc aatagcttcc
     7561 acctgacttt gattaatcac agctaatggc acattggggt atgattgcat caagtcttca
     7621 atttgtggta aaactgcatg acacacgcta cactgatcgc gcatgacatg tacaactgct
     7681 aacggatatt gatgaataaa ttcacggaat gctacgacac tatttaattg cttaaccatt
     7741 tcgaacacct tcttactttt tatattttaa atgcgatcat tattttgttg ctaaaatgtc
     7801 ttgaaatact tgtggcacag aggtaatctt cttatcgctc aatctgtaaa aaccgcctgt
     7861 ctgtgtacca atacaatgca cgccatgtgc ggacacaatt tcaaactgaa acacccattt
     7921 catcttgctt gctttaacta agtaaatacg acctactggt ttgtcatgta ttgtgacagg
     7981 tactttatac tctgcttctg ttttcataag tataggtgaa atgccttgat tgatcattgt
     8041 tgagtaattc atgtgctgat taataaacgc tgttctcaaa tcttcgaacc atctaatata
     8101 aacgatgtta ctaacgacac ccatcgcatc aatgtcgtaa ccattcactt cgatttcttt
     8161 ctctgcaact aatagacctt ctgttgtcat aatgacaaat ctccttatct tacaaataga
     8221 tttaaaatca tgttaattta tttttattac tttcatgata aaaatacaag caattgaact
     8281 ttattacgga aaatcaaact tttcgatgac gctattaatt tagctagtcg cgaagacgca
     8341 tttttctaaa atgtgacaaa aacataagca atgttttcta tacctaacac taatattatt
     8401 ctcatcaaca cacctgctat acatcattgc gcactaaaaa ggcagtagct tcattttaag
     8461 ctactgcctt ttgtaacata ttctctttca actcgctact tcgcatacgc actacgaatg
     8521 actaaaagtt cattcatttg atatgctgtt tcatttttaa tcattatttg accgtcataa
     8581 ttagcttacc tggatcagca tcttgtacat cttcaatgac aagtttttta ttttcaagat
     8641 ttgtaataat cattggtgta acgatgcttg gtgcgtgcgc tttcaagtat gctaaattca
     8701 ctttcattaa tggttgacct tgtgttactt tttcatcaac gttaatcaga ctttcgaatc
     8761 cttcgccatt cagtttcact gtatcgatac caatatgaat aagtacttcg acaccacttt
     8821 cagattctaa tcctatcgca tgtttcgtag ggaagattgt tttcactgta ccatcaaacg
     8881 gtgctacaat ttcacctttt tcagggataa agccaacacc atcacccatc attttaccag
     8941 cgaatacttg atcaggtact tctgataatg gaatgatttg accgacacct ggtgcataga
     9001 tgtctgttgt tccaagtgct tctacgtgaa ctggttcatc tgacatttct tcagtcactg
     9061 tcgtttcact tggctttgta atttcaccac tcataatctt cgccatatca tgtttaattt
     9121 gatctgattt tggaccaaag atagcttgca tattgtttcc aacttctaat acacctgatg
     9181 cgcctaaagc tttaatacct gctacatcta cttttgattt atcaaccact tctacgcgca
     9241 gacgtgtaat acatgcatct aaatgtttaa tgttttcttt tccacccatt gcatctaaga
     9301 catcaaatgg taattttgcg acactagagt tacgaatttc agtttcttca tcttcacgac
     9361 ctggtgtttt caatttaaac ttacgaattg caaagtcgaa taagaagtaa tacacgatag
     9421 catatacaat accgactgga ataactaata atgcgtgtga acgatcccag tttaataaac
     9481 catataaaat ataatctatg aaaccacctg agaatgtcat accaatttta acgcctaata
     9541 aatgcattac taagaatgat gtaccagcta ataatacgtg aattccataa agtactggtg
     9601 ctacaaataa gaatgaaaat tctaatggct cagtgatacc agttaaaaat gcagttaatc
     9661 ctgctgataa cattaaacca cccacgactt ttttacgttc tggtcgtgca tttttataaa
     9721 tagcaaatgc cgccgctggt aaaccaaaca tcatgaatgg atatttacca gtagtaaatg
     9781 caccagcagt aaatggtacg ccatctttca attgtgccat ccaaatacgt tggtcaccac
     9841 gaactaattc acctgcgtga tttgtataac ttccgaattc aaaccagaac ggtgaataga
     9901 aaatatgatg taaaccaaat ggaattaatg agcgttcaat aataccgaat ataaaagttg
     9961 ttaatgttaa atttttattt aataagaaat tcgataaact atttaatcca tcttgaattg
    10021 gtggccacgc aaaacttaaa accacacctg ttgcaattgc tacgaccgat gtcacaatcg
    10081 gtacaaatcg tttacctgca aagaatccta aaaatggtgg tagtgtaata ttataaaatt
    10141 tgttgtaaca ccatgcggct aaagcaccca tgataatacc accaaacaca cccgtttgta
    10201 atgttggaat acctaatact aaggcatgtg ctggttcttt cgctgcttga cttaattctt
    10261 ttgccccttt ggcatatgag aaaatgtcat caattgtaat atgcaacact ttccccattg
    10321 ttgcattcat aattaagtaa cctactagcg ctgctaatgc tgcaacaccg tctcctcctg
    10381 ctaatccaag tgctgtacca actgcaaata ataatggcaa gttatcaaat acaacttgtc
    10441 ctgctgcttc catgaccgac gaaatcatta caatgatatc gttttttaac catggtgcaa
    10501 tttctactaa ttgttcgttg tgcattgcgt taccaaacgc taataaaata ccagctgctg
    10561 gtaaaatcgc aacaggtaac attaatgctt taccgatacg ttgcaattgt ccaaacaatt
    10621 tcttaaacat ataaacatcc cctatctcaa tttccgcttc catgtacaaa aaagcaggca
    10681 tgaacaaaca agactattct catagtacgg ttgtttgctc atgcctgcat taccagtaac
    10741 acgcaagtga tatgtaattc atagctatta tacatatttg taaaagcgtt ttcaacaact
    10801 ttttcacaat tttatcatca attcagtaca atcatttgtg ataaataaac aaaacggcaa
    10861 ttcccatttt gttgaaatag aaattaccgt ttttatagtt caattcaatt gtgataacct
    10921 ttcaatcagt tataagaaac gttttgctgc aactgaaata ggtggaattt gatctaaatc
    10981 taaatgttta tcttcagtaa tagatctaaa tgcccattta ccataaccaa gcgcttcttc
    11041 atttacaatt ttacctggta atggcgcagc attaggatca acataaacat ctacaattgt
    11101 tggtacatct tgtgctagtg cctcttctac aatagcatct acttcgctag cacttttaat
    11161 tgtataacct ttaccacctg ctgcttcagc aaatttggca tgatccatat cagagaaatc
    11221 aaccgcatat tctaattcac cagcagcttg ttgttcatat ttaataaacg ctaattgctt
    11281 attatttaga acaaacactg ttaaaggtaa atcatattgt acagctgttg caaagtcctg
    11341 catcaccatt tggaatgcac catcaccagc aattgcgatg gcttgtctat ttggatatgc
    11401 aatttttgat gccattgcac ctggaagacc gcaacccatt gtacctaacc aacttgaaat
    11461 gatgaacttg ttatttacac caaggtttaa gtatcgagtt gaccaaactg ttgctgtacc
    11521 tacatctgct gaaatcactg catcatcttt aataaattta ttgattgatg ccattaatcg
    11581 ttctggacgt aatggtttac tattattatt tttatcttgt tccatccatt tatcccaaac
    11641 cgctttacgt tctaacgttt tgtttaagaa tggtctttca gcaacatgtt taatattttc
    11701 agttaactga tgcaacgcaa ttttactatc tccaacaatt cctacattaa tattgaaacg
    11761 atgtccgata tttttaggat ttgtgtcaat ttgaatggct ttaatatttt tcttaggtaa
    11821 gtaatccaca tatggatagt ttgtaccaac cataattaat aaatccgctt cctgcattgt
    11881 ttgataagat gttttagtac cgattttacc taagttaccg atactatatg gatgatcatc
    11941 cggcaagatt gttttagctg gtaatgaatg aatgacagga attttagtca tttcaataaa
    12001 ttcacgtagc tcatctttcg catgtttcgc accaacaccg attaacatga caggcttttt
    12061 acttttatta attaatttaa ccgctttttt gatgtcttta tattttggtg atacaactgt
    12121 tggtcttgat gtatctactg gtttattcgt tgtatcttta attttttcag ttaataagtc
    12181 gttaggacaa ataacaacag cgacaccttt ttgttcatat gccgtacgaa ttgcttcgtt
    12241 aacgatttca aacacattgt cacctttttc aatttggtga ttataaacgg ctacatcttc
    12301 acataatttt tgtaaatttg tttcttggaa tgctttcgtt ccaagtgctg tactattcgt
    12361 ttgtccagat aatattaatt gcggtacatt atccattttg gcatcgtaca taccatttaa
    12421 taaatgaatt aaaccagggc caccgatact taatgccaca ccgattttac cagttaattt
    12481 tgtataacca gcagccgcta agcttgctac ttcttcatga cgtacatgat aaaatttaaa
    12541 ttgatctctc actgtacgta aactatcgac aactgcgtcg attgagtctc ctggaatacc
    12601 atacaagtga tctatatccc atgcttgtaa tgctttaact aatgcttcat ttgcttttat
    12661 ttttgccatt attactaata gcctcccttt cttagtgtct ttttctaatt aatagaacag
    12721 tatgattaga acaggtatta acaccgaact aataactgca gttaaaatca tcccaattga
    12781 actaaatgca ccagattcaa tatccatctc cagtgcttta gctgtgccaa atgcatgtga
    12841 cgcattccca tacgttaaac ctttggcgat agaagattca aattttccaa atcgtaataa
    12901 catcgaacct aaaatactac cgattaaacc cgttgtgata ataaaaagta cggtcatcgt
    12961 atctgtgcca ccaagttcat gtgacacttc tataccaaca gcggctgtta tagatcgggg
    13021 caataatgtt acaatgacgt ctttagaata gccaaatgct ttaagtgtta agaacactaa
    13081 catgaaattt agcatgacgc cagttaatac acttgcaaaa atgatagaga cattatcttt
    13141 aattttctct ctatttttat aaagtgggta cgctaaacat acgacggttg cgtttaaaat
    13201 atggttgatc caactgccac ctttcatata cccgttataa ctaattccaa agataagtaa
    13261 gacaaaaata attcctaaag atgcaattaa tgctggattc aaaaatggat ttggatattt
    13321 ttgttgtaac cttttagcga aataatataa aacgacagtc aacaaaatca ttaacaaggc
    13381 ttgcacgtaa tcattcataa gcatctatac ctttacgttg cttatgtttg acagacattt
    13441 tttcagcaat ataacctgaa gataatgcaa cgatacatgt tcctatgata atgactgcga
    13501 aaaagagtat ataatttagc gtaatttcgg aagcaacatc cataatacct acaactgacg
    13561 gtatgaagaa aaagaccatc gtctttaata aaaagtttgc accgtcttct acccaagtta
    13621 gcggtacaat cttaaattgt aatagtaaat aaaataaaaa tagaccaaca atactgccgg
    13681 ctaagggaag atgaaaaatc ttttgaattt ctgtgccaat ataagtaatc acaatgatga
    13741 ttcctagttg tagtaataat ttgattatta attggacttt gtgcatggcg ccatcccttt
    13801 ctaaatatgt ctaaattgtt acaataacta ttataaagat ggcgtacaca atataaaaat
    13861 actctttttt tatagtttgt attactattt tctatggaaa cgctctctaa atttcacttc
    13921 attttcacaa aaaaacactc attatttcaa tggcccacgt aatgatgcgc cttaaataat
    13981 gagtgcttta ttaatatgtt agcctaaacg atctttcaaa aattctatcc atttcgttgt
    14041 ggcatgactt aattgtttat ccttcttcca aacaacacct aattcccaat gtacatgcgc
    14101 atcttcaatg cgtagtagct tcacatctcc atttagttgc tctgaaattg atgttggtaa
    14161 aatactaata cctaattcat tcgtaactaa atcttctata acatgccatt gtgaaatttg
    14221 cgctacagta ttcggaacaa agccaacgtt tttagcattt tcaataattt tatcattcaa
    14281 gtaaaagtct ttattaaata aaatgaagtc ttcacctgct aaatcttcga gtttaacagt
    14341 ttcatatttt gcgagtcgat gctctctgct cacgataagt cgcaaatctt ccttatctag
    14401 ggtagtataa tcgaaaatat gatgatccac tggcaaagtg gtcacgccta tatctacttc
    14461 atcattgata atttgctgct caatcgtttt tccgccattt tctattaaat tatatgtaac
    14521 atttggatat tgttgatgga atgcaccaag aatattgata agaatcttca tattcatgac
    14581 tgccgacatg cccatattta tatgccctgt ttccaatcca ttcaagcgtt ccatttcaga
    14641 tggtaaataa tcatacagtg caacaatttc tttacttttc tcataaaaaa tttgacctgc
    14701 atcggtaagt attaaatgtc ttttacttct atcaaataag ggcgtgccca tttcattttc
    14761 aatatcttta attgctttac taattgtagg ttgtgcaata tataatgatt tggaagcatt
    14821 agtcatgcct ccttgcttaa cgacttcaat aaaatatttc atatgtttga tatccactaa
    14881 catcatccta actatttcaa cacgtttatt tcattataca caacttctta aacattcaat
    14941 aaatttagat ttataaaatt gctttataaa aagttttatg taaaatacac attcttttat
    15001 ggtattatat taatggctca agtttgttat tcgaggtgag gtattgagct tttacgaatt
    15061 tatgcaaagt tttcttggtg atgacacacc attaggtgaa ttggttgatt ggattaatca
    15121 agatagcaat ttccctagag aagtgaagag ccatagtgaa atattgtcat attttcgaac
    15181 aaatccatgt cctgaaagca tctcagtacc tgtaattaaa cgggcactat cagttttcaa
    15241 ccaattcaca aatgtacaat gatacatgaa tacatggagt cacatcatct catgttagcg
    15301 ctctgattgc agggcgctgt ttttaatgtt atcgcttcat ttatatgttg tgattgctat
    15361 tttcttttag ttatagaacc acgtaccttt tcttttaaat cattttatat tttcccacta
    15421 atatccgctg ttaatcaatc ctgacatcct tgtatcacta tgacacttaa ttgttagata
    15481 catgaatttc tgcattttat gaaaaaatcc atttttatta caatttaaca ctttatatga
    15541 cagcttcatt acagttactt ttattgttga ttgcttgcat tgttttctaa aaaaattttg
    15601 ctatcataaa taacgttgaa taaagaaaaa attaacttgg gagataaaaa tgaaatataa
    15661 aaagatactt tttcgtttac taattgcttt tgcagtcctt ttctcagcag atttcactta
    15721 tcaatccgtc gaacaaacgc atcaatcgca tgccgcagtt aattattata gtaaaaacca
    15781 atgtacatgg tgggcattta aacgtcgtgc acaagtcggt aaacctgttt ctaatagatg
    15841 gggcaatgcc aaaaattggt attacaatgc acgtaaatca aaatatgcga ctggtcgtac
    15901 accaagaaaa tttgctgtca tgcaatcaac tgcaggatat tatggacatg tcgcagttgt
    15961 tgaacaagta tataaaaacg gtagtattaa agtttcagaa tacaactttt atcgcccatt
    16021 aaaatacaat acacgtgtac taagcaaaaa ggcagcacgt aactttaact atatttacta
    16081 atcaaaaaac ttctatcacg aacgcttcaa attcctgtat gcgtgtgata gaagttttta
    16141 ttttttgttc atattaattt atctaagcgc tacgatggac tgacttaaac ttttctttaa
    16201 aaatcgctat tcgcatttga ttattgttgg cttcttaaat cttgtaaaat gcgttcagct
    16261 acttgtgtat tcgcacgagg ttcttgcttc aatgcttcag ctacttgcgc aatttcatca
    16321 ccttttgcac ctacaacaat agctaaagat ttatattgca agctcatatg gccttgctgg
    16381 ataccttcgg aaacgagcgc gcgacatgct gcaaagtttt gcgctaaacc aacggcagca
    16441 actacatgac ctaattcttg tgctgaatct acatttagca attctaaaga agctttagca
    16501 attggtaata cttttgtacc accgccaacg attgccaatg tcataggcac ttctattgta
    16561 ccaattaaac gttgacgttc ttgatcgtat ccccatgttg caataccacg atattgtcca
    16621 tctctacttg cgtatgcatg cgcacttgct tctgcaccac gcgtatcatt tcctgttgct
    16681 aaaacaacgg catgtatgcc attcataaca cctttattat gtgttgcagc acgatgaata
    16741 tcaacttgtg ccaatacaga agcacgttcc attcgtttgg caacctcttc tccagttctc
    16801 tcgccccttg ctaaatcttt aacatcaatt tcgccttgaa ctttaacaac ggacgctgtt
    16861 gcatgattgg ataaaatact cattaaaatg tcgctttgcg gaaattcatt ttttaaaaat
    16921 gcagttatgg cctctaaaat cgtattgagc atattagcgc ccatagcatc tttcgtatca
    16981 acaaatactt ttaaagatag taactgttgc tcaggaaatg tatcaatagc tatacgttgg
    17041 taaccaccac cacgcgcttt aatagaagga tatgcctcat ccgcaatttt atgaatttgc
    17101 ttttctaaag ctttaatgtc tgctgataat ttttcagtat cgtcaacgcc atcaaagacg
    17161 atttgaccta tcataatacg ttcagaagat accgttttaa atccgccagt ctgattcact
    17221 agctttgcac cataactagc tgcagcgaca actgaaggct cttccaccat cataggtaca
    17281 acatatgcct tatcgtccac aatgatattt ggtaataatc caacgggtaa tgcaccttgc
    17341 gcgatgacat tttcaattaa actatttgct acttcctcat caattaatgg atgattcaat
    17401 aaaatgttga attgctcttc tgataaccat tgcttatcta ccaattgttg taacttttgt
    17461 tgacgagata aatgtcggaa attcttatct aaactttgca tggacatact ccttttactt
    17521 cacataattt tttaacattt caatcactac tatttttacc ataaaataac gttattcgtc
    17581 ttaaaattca attgaataat tattgtttta actataaaat aaagcaaggt aaattaaaac
    17641 gcttacaaga aacgtcaaat catttttaaa tttagtgtat ttctttgtat aagattagca
    17701 tattctgata tgatacaagt gttgctttta taaatttgaa aggatgtaaa accttatgac
    17761 aataggtatc gacaaaataa acttttacgt tccaaagtac tatgtagaca tggctaaatt
    17821 agcagaagca cgccaagtag acccaaacaa atttttaatt ggaataggtc aaactgaaat
    17881 ggctgttagt cctgtaaatc aagacatcgt ttcaatgggc gctaacgctg ctaaggacat
    17941 tataacagac gaagataaaa agaaaattgg tatggtaatt gtggcaactg aatcagcagt
    18001 tgatgctgct aaagcagccg ctgtgcaaat tcacaactta ttaggtattc aaccttttgc
    18061 acgttgcttt gaaatgaaag aagcttgtta tgctgcaaca ccagcaattc aattagctaa
    18121 agattattta gcaactagac cgaatgaaaa agtattagtt attgctacag atacagcacg
    18181 ttatggattg aattcaggcg gcgagccaac acaaggtgct ggcgcagttg cgatggttat
    18241 tgcacataat ccaagcattt tggcattaaa tgaagatgct gttgcttaca ctgaagacgt
    18301 ttatgatttc tggcgtccaa ctggacataa atatccatta gttgatggtg cattatctaa
    18361 agatgcttat atccgctcat tccaacaaag ctggaatgaa tacgcaaaac gtcaaggtaa
    18421 gtcgctagct gacttcgcat cactttgctt ccatgttcca tttacaaaaa tgggtaaaaa
    18481 ggcattagag tcaatcattg ataacgctga tgaaacaact caagagcgtt tacgttcagg
    18541 atatgaagat gctgtagatt ataaccgtta tgtcggtaat atttatactg gatcattata
    18601 tttaagccta atatcattac ttgaaaatcg tgatttacaa gctggtgaaa caatcggttt
    18661 attcagttat ggttcaggtt cagttggtga attttatagt gcgacattag ttgaaggcta
    18721 caaagatcat ttagatcagg ctgcgcacaa agcattatta aataaccgta ctgaagtatc
    18781 tgttgatgca tatgaaacat tcttcaaacg ttttgatgac gttgactttg acgaagaaca
    18841 agatgctgtt catgaagatc gtcatatttt ctacttatca aatattgaaa ataacgttcg
    18901 cgaatatcac agaccagagt aatctgtgta tttgaaagac atttaaatac aacctaaaaa
    18961 cagcagtacg acagcttctc attgaaatcg tcttactgct gttttctatt tataatgctt
    19021 cgtcttgaat gaattcatta tgcctatttg acacattatt gaagttttcc taatgcctgg
    19081 atcatttata atttacggct tcgtgctatg ttttggtaca taaagctttg acatatcgat
    19141 attctccaac tctaacaact taattttatt attaatcgtt ccaccgaatc ctgttaagct
    19201 acccgtttta ccgacaacgc gatgacatgg cacgataatt gataatggat tgcttccaac
    19261 tgcacctcca accgcttggg ctgacatttt tggcttatca agcagcttgc ctactttttt
    19321 ggcaatagca ccatacgttg ttaacgtccc ataaggaatc tgtcttaatt cgttccaaac
    19381 acactgttga aaatgactac ctgttggctt taaaggtatt gttatttcag gattatcgcc
    19441 tttaaaatac gcgtctaacc actgtgtcgc ctctttaaat atcgctaaag acgtattttc
    19501 ttccctagta ccatcatctt gttgattttc aaacaaaaca gcggtcagac ttaccccatc
    19561 actcacaagt tccaatcgtc ctacaggaga atcataatag cacttataat ccataaaaat
    19621 gccccctttt tctaatcata cgccttaatt gttaatttag aaaatattca tgttcacatt
    19681 ttttaaaaat aaataagtcg tcaattacgt atataaacac gtaataccag ctatcccttt
    19741 actgcaatat acagttacat aacttactac acgtgctaac ctcttacttt gtaaatcaaa
    19801 tcttaaatta aaatattgaa aatgcaatgg atccttaata ttttattaaa catatattta
    19861 cttattaaaa acaacacaca atattcataa agttttaaaa atattctgtt ttatcaccta
    19921 ttattagtgg aaaagtacaa ttgcaattgt atatagtttg cataacgctt caaaagtaat
    19981 ttcttttttg tttagttcaa aaaaatttag aggtgatgtt atatgaataa cggttttttc
    20041 aatagcgact ttgattcaat ttttcgaaga atgatgcaag atatgcaagg ttcaaatcaa
    20101 gtcggtaaca aaaagtacta tattaatggt aaagaagttt cacctgaaga actagcgcaa
    20161 ctcacacaac aaggtggcaa tcactctgct gaacaaagtg cgcaagcttt tcaacaagca
    20221 gcacaaagac aacaagggca acaaggtggc aacggcaatt atttagaaca aattggccgt
    20281 aaccttacgc aagaagcacg tgacggttta ttagatccag tcattggtcg tgataaagaa
    20341 attcaagaaa ctgctgaagt tttaagtaga cgaactaaaa acaatcctat attagttgga
    20401 gaagctggtg ttggtaaaac tgcgattgtt gaaggtttag cacaggcaat cgttgaaggg
    20461 aatgtacctg cagccatcaa agataaagaa attatatcta tagatatttc atcgcttgaa
    20521 gctggaacgc aatatcgtgg cgcctttgaa gaaaatattc aaaaattaat cgaaggtgtt
    20581 aaatcttcac aaaatgccgt actattcttt gatgaaatcc atcaaattat cggttcaggt
    20641 gccacaggaa gtgattcagg tagcaaaggg ttatctgata ttttgaaacc tgcattaagt
    20701 cgtggtgaga tttctattat tggtgcaaca acacaagatg aatatcgaaa caatattctt
    20761 aaagatgctg cattaacgcg cagatttaat gaagtacttg ttaatgaacc aagtgctaaa
    20821 gatactgttg aaattttaaa aggtattcgc gaaaaattcg aagaacacca tcaagtcaaa
    20881 ttaccagatg acgtattaaa agcatgtgtt gacttatcaa ttcaatatat tccacaacga
    20941 ttattaccag ataaagcaat cgatgtgtta gatattacag cagcacattt atctgcgcaa
    21001 agtccagctg tcgataaagt tgaaactgaa aaacgaattt ctgaattaga aaatgataaa
    21061 cgtaaagcgg taagtgctga agaatataaa aaagctgacg acattcaaaa tgaaatcaaa
    21121 tcattacaag ataaattaga aaatagtaat ggtgaacata ctgctgttgc tacagttcat
    21181 gatatttcag atactattca acgattaact ggtattccag tttctcaaat ggatgataac
    21241 gatattgaac gtttaaaaaa tatttctaat cgtttaagaa gtaaaatcat aggtcaagat
    21301 caagctgtag aaatggtttc acgtgcaatt cgccgtaatc gcgctgggtt tgatgacggc
    21361 aaccgtccaa ttggtagttt cctatttgtc ggccctactg gtgttggtaa aacagagctt
    21421 gctaaacaat tagcaattga tctatttggt aataaagatg cacttattcg acttgatatg
    21481 agtgaatata gtgacacaac agctgtttca aaaatgattg gtacaactgc tggttatgtc
    21541 ggttatgacg acaattcaaa tacgttaact gaaaaagtac gccgtaatcc atactcagtc
    21601 attctatttg atgaaatcga aaaagcaaat ccacaaattt taacattatt actacaagtg
    21661 atggatgatg gtaatttgac tgatggtcaa ggtaacgtca tcaactttaa aaacacaatc
    21721 attatttgta cgtcaaacgc tggttttggt aacggcaatg acactgaaga aaaagacatt
    21781 atgcatgaaa tgaaaaaatt cttccgccct gaattcctta accgcttcaa cggcatcgtt
    21841 gaattcttac atttagataa agatgcatta caagatatcg tcaacttatt attagacgat
    21901 gtacaagtta cattagataa aaaaggtatt acgatggacg tttctcaaga tgcgaaagat
    21961 tggttaattg aagaaggtta tgatgaagaa ttaggcgcac gcccattaag acgtattgtt
    22021 gaacaacaag tgcgtgacaa aataacagat tattatttag atcatacaga cgttaaacat
    22081 gtggatttag atatcgaaga caatgaatta gtcgtcaaag gtaaatcatg atactttaac
    22141 atatagcgta tcaaaaaatg agcatcagga cgccctcgcc tgtgctcatt ttttaaatta
    22201 tttcccagga aaatgattcg ctgtgtgttg ttctgttcca caacaatcac gatcaatgtc
    22261 acatgtacca cattttcctt gtttcgaacg tttgaaaaat ttaactaacg tatacaaggc
    22321 atatccgaaa atcgctaaaa aaattaaaat attaataatg actgacactt taaccactcc
    22381 ttaaacaaat aaatgtccaa cttgataaaa gatgaatgtt aagacatatg cagtgactag
    22441 aggataggca actgcaagtg ccgtccattt ccatgaataa gtctctttac ggattgctgc
    22501 cactgtagaa acacaaggaa tatacaatag tataaatatc ataaatgcat acgcagatag
    22561 cggtgtgaat tggttttgaa tcacattaac aaggcctgca tcacctgatg aatagataat
    22621 cgccatcgaa cttacgataa cttcttttgc taaaaatcct ggccctaacg tagcacctgc
    22681 ttgccatgtt ccaaatccga gcggttgcac taacatacca aagaagctac caactatatg
    22741 taaaaaactt tgattgatat tcacattgat accatgtggt cctatataac ttaatagcca
    22801 aatgactact gagccgccaa aaataaaagt acctgcttta cgaacaaagc ccttagcctt
    22861 ttcccaagta ctacgccaca acgttttaat ggaaggcaca cggtatgttg gcaattccac
    22921 aataaagatt gcattatcat tttttaaaac cgtcttagta agtactgtac tgactaaaaa
    22981 tgccataata atccctaaaa catacaagct taatactact aaagattgat tctctttgaa
    23041 aaagatacct ccgaacaatg catacactgg aagtctagca gagcatgaca tgaatggtgc
    23101 aattaatatc gttgttaaac gctctttttc attttcaata ctgcgcgcag ccataatact
    23161 cggtacgtta caaccaaatc cgataatcat cggtataaaa gacttcccgc ttaaaccgaa
    23221 agattccata atacgatcca ttaaaaccgc aatacgtgcc atataacctg aatcttctaa
    23281 taaagatata aagaaaaaga gcacaacaat ttgtggtaca aagactaata ctgatcctac
    23341 accagcaata atgccatctg taattaaatc ttgtaaaaat ggtataacgc caagataatt
    23401 cataatcgtc ttcacactat ccgtaaatgt accacctata aatgcatcga gttgatccga
    23461 caaaggtgtg ccaatccatg taaatgtagt ttgaaagatc aaccacataa tcgctagaaa
    23521 aataggcatc cctatatatt tatgtgttaa tatcttgtct attctagagc tgaaatattg
    23581 cttatcttca tctggatacg ttaccacgtc ttgcaataac gtctcaatat aatgattgcg
    23641 tatacgttcc atctctcgac gaacagatac agcctctact tgttcagcaa cttgatcacg
    23701 taaactcgac aatttattta caacctctga attaagttca tttgcaattt gcatgttatt
    23761 tagtaagaat tgaatcgcaa taaatctagc ttgatattta tcatgagatg tctctgtcat
    23821 tattatttga cacatatttt taattgtctc ttcaatcttc tcatcataat tgattttaaa
    23881 atgcggttga tacccttccc ctagatgctt tatttcgcca agtaaatgtt tagttccttt
    23941 gcctgtacgt gccacaactg gaaaaatagg tgtttttaac tttttcatca atttatgata
    24001 atcgattttt atcccgcgct ttgtagctac atcaatcata tttaatccga tgtatattgg
    24061 ttgattaagt tctaacaatt gtactgttaa ttgcatattt ctttttagtt gactcgcatc
    24121 aacaatgtta atgattcctg aaaaagaatc gtttaataaa tagtctgtca ctacagtttc
    24181 atctttagaa atcggcgata aatcatatgt acctggtaaa tcaattaatt gtcctacatt
    24241 ttctttaagc ttccctactt ttttctctac cgttacgcca ctccagttgc ctatatattc
    24301 atacgaacca gttaaagcgt taaacaaaga tgttttacca acattaggat ttcctaaaat
    24361 acaataattt tccattcgtc cggctcctat tcttctaatg caatagaaca agcatcgcaa
    24421 tgtctaatac ttaactgttg tccgtttact tcaataatac atggcccttt aaataaacat
    24481 ttttgtttaa tcgttatgat agcgtcatct gttaacccaa aggcacttag acgatacaac
    24541 atattctcat tagcaatatc cattcgcttt attttataag ccttattcat ttcaccattt
    24601 ttaatgttta acatactatt tcgctctcct attagaaata ataatcatta tcacttaaaa
    24661 atcataaccc ttaaaattgt agctcgcaat actttattta aataattttc atttttcatg
    24721 taaaatttgt gacattgcaa aaatgttttg tataatcgct tccatgcaaa aaacctccgc
    24781 ttcaaatgtc attgatagac atttaaagcg gaggcatgta tatgttattt tatgatttat
    24841 ttcttaagtt tttttctaac aattgattga gcttttcaat atttttattc aactgttcta
    24901 tattcgtggc ttgttgtttt gaatttagtt gatgcttgtt ttgataatcc attaaagcat
    24961 catataagaa caatgaactt gctgattgct gtttcacttc ttggtacaat gccatcgttt
    25021 tatcatctag tactattgaa cgtacatcat cttgcccatg tacatcttga tttttcgata
    25081 catttgttgc accttgtgga tatactttaa aggttgtgtc gtatgtttcc ttactatctt
    25141 tagcttcaga ttcctgtgat tcaaccgttt tatatttttc aagtgcatgt ccttcaatat
    25201 caactcgtgg aataatgcgg ttcaaccatg ctggtaaata ccacgaacct tttccaaaca
    25261 atttagttaa tgcaggaatt aacatcattc tgactacgaa ggcatcaaag agtacaccaa
    25321 acgctaatgc catacccatt gatttaatca tgacatcttc ttggaataca aacgcaaaga
    25381 agacactaaa catgattaat gcagctgcta caataacagg accactttct ttcaatccta
    25441 ctttgataga ataatcatta tcccctgttt tactatattc ttcatgaatt cgcgacataa
    25501 ggaagacttc gtaatccatt gctaagccga atagtatacc aattgtaata accggcaaga
    25561 aggctagcat tggtcctgtc gtttctatgc cgaataatcc tttcataaag ccatcttgca
    25621 tcacaaaggt tgtaaaacct agtgttgcca ttaatgacaa gacaaatcct aaaactgctt
    25681 ttaacggtat tagaattgaa cggaagacaa tcattaataa gaaaaatgcc aatacaacaa
    25741 ttactgttgc aaataaaggt atcgcctcat ttaacttttt agacatatca atattaatga
    25801 cactttgtcc tgaaatttcc gttttaaatc catatttatc ttgcgcatct ttattataat
    25861 ctcgtaaatc atgtactaag tcatttgtac tttctgcatt aggtccatgt ttaggtatca
    25921 caaccatcaa agcgtaatca ttatctttac tcatttgtgg cggcgtaaca atatctacat
    25981 ttttcttatc tttaatatct ttatacacag attgtaaatc ttgttgtaat ccttgtggat
    26041 catctttttt atctttaacg ttaatcagca tcggtatttg gccattaaat ccttcgccaa
    26101 atttatccga gataatatcg taagcttttt tctgtgtaga atctgctggt ttaacaccgt
    26161 catctggaat gccaagacgc atatgactaa ctggaatagc cgctgcaact aatataatta
    26221 aacctaataa tactgctgca agtgcattac cagtaataaa tttagaccac ggcgtatcaa
    26281 tatctttttt aaaattagac tgcaatttgt tcactttaat gcgtttatgg aaaatgctaa
    26341 ttaacgcagg taataaagtc agtgtactga atacagcaaa tataacacta attgccgaag
    26401 caaaccccat cactgctaag aaatctatgc caactagcga taaaccacat acggcaatca
    26461 caactgtcac accagcgaag atgactgcgc tacctgctgt tcccactgca agtccgatac
    26521 ctttaacaaa gtctgtttca gttttcatta cttgtcgata tctaaacaaa ataaataatg
    26581 cataatcgat accaacagcg agcccaatca ttaccgctaa agttaatgta acattaggta
    26641 tatcaaagac ataggtcaat aggctaataa tgccaactcc agatgctaaa ccaatcaatg
    26701 cacttataat tggtaaacct gcagcaataa ctgaaccaaa tgtgattaat aacacaacaa
    26761 aggcaacaat aataccaact aattcagaat taccgcctac ttctgtagat gtcatacctg
    26821 ttcctgttaa ttctgtttgt acattatgat tatcacgtaa atcttttaaa tgatttttaa
    26881 cattatctct agacccatct tttaaagatg tttgactaac gtcatatgtg atatctgcaa
    26941 atgctgttgt tttatcttta ctaatttgct tattttcata aggatctgat attttatcaa
    27001 tgtgcttgtc atctttttta atatcatcaa acgttttctt aatatcttta gtaatactcg
    27061 gttgtagaat tccatcatct ttagtcgtct taaagacaac acgtatttgt gccttttcac
    27121 tatcttgatt aaaatgtttt tcaatctttt tattcgtatc taacgactct aatcctgtca
    27181 ttttaatatc attgtcaaat ttcggtgcat ttgtagcaag tggtatcaat attgcagcta
    27241 caataattac ccatgcgatt aatgtggacc acttatgttt tgcgatgaat gtccccatct
    27301 tatataaaaa ttttgctaaa gtatattgcc tccttttaaa atcaacgtta tagtttaact
    27361 atacagtgta gattattgtt tgattatagt atctatcccc gacctcttaa agaatcaatt
    27421 ggaaaatttt gtatattaaa ctacacacaa aggagaaatg tagatgaaag agactgattt
    27481 acgagttata aagacaaaaa aagcattgtc aagcagcttg ctgcaattgt tagaacagca
    27541 attattccaa acgattactg tcaatctaat ttgcgacaac gcactcgtac accgcacaac
    27601 attttataaa cacttttatg ataaatacga tcttcttgaa tatttattta atcaattgac
    27661 taaagactac tttgctagag acattagcga tcgccttaac cacccatttc aaacgattag
    27721 cgatacaatt aataataaag aagatttgcg agatatcgca gaatttcaag aagaagacgc
    27781 tgaatttaat aaagtattaa aaaatgtctg cattaaaatt atgcataacg atatcaaaaa
    27841 taatagagac cgtatcgata ttgacagcga catcccagat aatctcatat tttatattta
    27901 tgactcgttg attgaaggtt ttatgcattg gataaaagat gaaaaaattg actggcctgg
    27961 tgaagaaatt gataaaattt tccataaggt cattaatatt aagattaaat agtaaattag
    28021 aggctcataa gcgttaccaa cattcataat aaaaacgata gtttacacat taatgaattc
    28081 gtgtactact atcgtttttt tatttttatc gtgcttatcg ctattaaaat aactgataca
    28141 caacacataa actatgaaga aaaaaataaa tccgctatct aaatgacttt gactcagttg
    28201 tttaaatgac caaattgcta atgcaattcc cattattatt gaaataacgt atctcacatt
    28261 cttataccta taagcctttt ctaaaaatat ggttgctatt acttaatttt taaagttata
    28321 aataaaaaga gccaaccgca atggatggcc cttgttcatt atgaagcatt agaacatttc
    28381 tgaaacaacc ttttgttcta agaagtgtaa taagtagtct gggctacctg ttttagcgtc
    28441 cgtacctgac attttgaaac caccaaatgg atggtatcca acaactgctg aagtacagcc
    28501 tctgttaagg tataaattac ctacatcaaa ttcgtttacc gctttaatcc aatgctcgcg
    28561 attatttgta atcactgcac cagttaaacc gtaatctgta tcatttgcaa cctcaattgc
    28621 ttcatcaaaa tcgttaactt tcacaaagcc aacaactgga ccaaaaattt cttcttgcat
    28681 gattctatct ttagatttaa gtcctgaaat gattgttggt tctacaaagt aaccttttga
    28741 atcatcagtg ccgccacctt gttctaattt accttcttct ttaccaattt caatataatt
    28801 tttaatctta tcaaattgtt ttttattaat aactgggccc atatacgtat tgtctacagt
    28861 attgcccaac gttaattctt ttgttagttt aattgatttt tctaatactt cgtcataaac
    28921 gtctttatgc acaattgcac gtgaacatgc tgaacatttt tgaccagaga aaccaaacgc
    28981 tgacgttaca atagcttctg ctgccatatc tgtatcaata ttttcatcaa ctacaatggc
    29041 atctttaccg cccatttcag cgataacacg tttcaagaag ttttgacctt cttgaacaac
    29101 ggcactacgt tcataaattc tagtacctgt cgcacgtgat cctgtaaatg taacaaaatg
    29161 cgtatcttta tgatcaacta agtaatcacc aatttctttc ggatcaccag gaacaaagtt
    29221 aactacgcct tttggtaatc ctgcttcttc taaaatttcc attaatttat aagcgatata
    29281 aggtgtatcc tcagcaggtt tcaataacac tgtattacct gcaacaactg gtgctaaagt
    29341 cgtaccagcc ataatcgcaa acgggaagtt ccacggtgga attgtaacac ctgtaccaat
    29401 tgatttatag aaatatttat tgtgttcacc ttcacgatca agaactggct taccttgagc
    29461 caaatccatc attgaacgcg catagtattc aataaaatca ataccttcag ctgcatcacc
    29521 aactgcttca tcccatggct tacctgcttc ataaaccata attgctgcaa tttccgcttt
    29581 tcgacgacga ataattgccg aaacacgtaa cataagctct gcacgatcat ttgctgacca
    29641 tgttttccaa gatttataag cttcgttcgc tgctttaaat gcatcttcaa catcttgttt
    29701 tgttgccttt gatgcatttg caatcacttg tgatgtgtct gcaggattga ttgatttaat
    29761 tttgtcatct ttgaaaatct tctctccatt aatcactaat ggtatgtctt gacctaattc
    29821 tttttccacg tctttcaatg ctttcttaaa catattcaca ttttcttgga ctgaaaaatc
    29881 gtaaccaggt tcatttttaa attctactac catgtacact taccccctat aaattttgaa
    29941 agtggtttaa ccctttgatt taatgatata acatcattta aactcatttt actatgagta
    30001 aggtcgtttt tgcaatcgct ttcattttta tacttcatcc cttattctca agtattttga
    30061 aatggattgg ttacttttta aaatttagaa gtgtcatgac aactaattta gtgtttcatc
    30121 gttcaatgct tctgatggta cctcattatc aattttacga acgactttac aagggtttcc
    30181 aaccgctaaa ctgtgtggcg gaatatcttt ggtgacaaca ctaccagcac caatcacact
    30241 gccttctcca atcgtcactc ccggaagcac ggctacatgt ccgccaaacc aagtattact
    30301 gccaatatta attggtcctg ctttttcaaa tccttcattt ctatgatgaa aattaagtgg
    30361 atgtgttgct gtgtagaatc cacaattagg tcctataaaa acattatcgc caattgtaat
    30421 ctgtccacca tccataaaat aacaattggt gtttacatag acattttttc ctagtttaac
    30481 gttccaacca taatctgtat caaaaggaat cgaaatactt acattgtctg ttgttgtttg
    30541 aaataattca tcgattaatt cctttctttt atttttgtca ctcggctttg tatgatttaa
    30601 ttcaaagcaa atatctttcg ctcgtgcacg ttcattgatt aagtcttgat caaagtttgc
    30661 atcataccat ttttctgcta acattttttc tttttcagtc attacacctt tcaactccta
    30721 ataacttatt tacttgttta atagttaatc aaataaacat tcgcctatgc aactaatacg
    30781 ctataacatt atgaaatcat gaccttatca ctcttatcta taccattctc gcatcaaata
    30841 ctgctaaagt agtagataaa ttcaatacta cagacgcatt cattttttaa tctattaacg
    30901 taaaatgtga gtaagagaaa tataaaggag tatgatagcg atgagaatat taattacagg
    30961 cacagttgct atcttaatca ttctaggttt agttaaaacg atacaagatt acgaaatgac
    31021 aaacgacaca agtcgtcagt tgtcagacaa caaagatgac gataaagtca tccatcttaa
    31081 taatttcaaa aatttacatg cgaaggaatt taacccatct gatttctttt aagtcaccta
    31141 agaattgcaa atccagaagt catttaagtt ttacctttca ttcatacatc ctttaatatt
    31201 aattacgact tcttttatat agatgctaaa tagagagatt gttgtgcaat gtttgcacgg
    31261 caatctctct ttttcttttt aaatttggta aaagtaaaac gcaacgattg acttatatac
    31321 ctataggggg tacattagac gtgtaacaat gaatcacagg gaggcaataa tgtggctaat
    31381 acgaaaaaaa caacattaga tatcactggt atgacttgtg ccgcatgttc aaatcgtatc
    31441 gaaaagaaac tgaataaact tgatgacgtt aatgcccaag tgaatttaac tacagagaaa
    31501 gcaactgttg agtataaccc tgatcaacat gatgtccaag aatttattaa tacgattcaa
    31561 catttaggtt acggtgttgc tgtagaaact gtcgaattag acattacagg catgacttgt
    31621 gctgcatgct caagccgtat tgaaaaagtg ttaaataaaa tggacggcgt tcaaaatgca
    31681 acggtcaatt taacaacaga gcaagctaaa gttgattatt atcctgaaga aacagatgct
    31741 gataaacttg tcactcgcat tcaaaaatta ggttatgacg catctattaa agataataat
    31801 aaagatcaaa cgtcacgcaa agctgaagcg ctacaacata aattgattaa gcttatcata
    31861 tcagcagtat tatctttacc gctattaatg ttaatgtttg tgcatctttt caatatgcat
    31921 ataccagcac tatttacgaa tccatggttc caatttattt tagctacacc tgtacaattt
    31981 attattggat ggcaatttta tgtaggtgct tataaaaatt taagaaatgg tggcgccaat
    32041 atggatgtac ttgttgctgt tggtacaagt gcagcatact tttacagtat ttatgaaatg
    32101 gttcgttggc ttaatggctc aacaacgcaa ccgcatttat actttgaaac aagcgccgta
    32161 ctaattacct taatcttatt cggtaagtat ttagaagcta gagcgaagtc tcaaacaacc
    32221 aatgcgcttg gcgaattatt aagtttacaa gctaaagaag cacgcatttt aaaaaacggt
    32281 aatgaagtga tgattcctct aaatgaggta catgttggag atacacttat cgttaaacca
    32341 ggtgaaaaga tacctgttga tggcaaaatt attaaaggta tgactgccat cgacgaatct
    32401 atgttaacag gtgaatctat ccctgttgag aagaatgttg atgatactgt aattggttca
    32461 acgatgaaca aaaacggtac tattactatg acagcaacaa aagttggcgg ggacactgcg
    32521 ttggcaaata ttattaaagt tgtcgaagaa gctcaaagtt ctaaagcgcc gattcagcga
    32581 ttggcagata ttatttctgg ttatttcgtt cctatcgttg ttggtatcgc actattaaca
    32641 tttatcgtgt ggattacttt agttacacca ggtacatttg aacctgcact tgttgcgagt
    32701 atttccgttc tcgtcattgc ttgtccatgc gcattaggac ttgctacacc aacttctatt
    32761 atggtaggta ccggtcgcgc tgctgaaaat ggtattttat ttaaaggtgg cgagtttgtt
    32821 gaacgcacac atcaaattga taccatcgtt ttagataaga cgggtaccat tacaaatggt
    32881 cgtccagtcg tgacagatta tcatggtgac gatcaaacgc tacaactact tgctactgct
    32941 gaaaaagatt ctgaacaccc attggcagaa gccattgtca attatgcaaa agaaaagcaa
    33001 ttaacattaa ctgagacaac aacatttaaa gcagtacctg gtcatggaat tgaagcaacg
    33061 attgatcatc accatatatt ggttggtaac cgtaaattaa tggctgacaa tgatattagc
    33121 ttgcctaagc atatttctga tgatttaaca cattatgaac gagatggtaa aactgctatg
    33181 ctcattgctg ttaattattc attaactggt atcatcgcag tggcagatac tgtaaaagat
    33241 catgccaaag atgctataaa acaattgcat gatatgggca ttgaagttgc catgttaact
    33301 ggcgataata aaaacactgc tcaagccatt gcaaaacaag taggcataga tactgttatt
    33361 gcagatattt taccagaaga aaaagctgca caaattgcga aactacagca acaaggcaag
    33421 aaggttgcga tggttggtga cggtgtaaat gatgcacctg cattagttaa agctgatatc
    33481 ggtatcgcca ttggtacagg tacagaagtt gccattgaag cagctgatat tactattctt
    33541 ggtggcgact tgatgcttat tcctaaagcc atttatgcaa gtaaagcaac cattcgtaat
    33601 attcgtcaaa atctattttg ggcattcggc tataatattg ccggtatccc tatagctgca
    33661 ttgggcttac ttgcgccatg ggttgctggt gctgcaatgg cactaagttc agtaagtgtt
    33721 gtcacaaacg cacttagatt gaaaaagatg cgattagaac cacgccgtaa agatgcctag
    33781 attccttaat aatgaaggat tcgttggtga ttctgagata ggctagtgat tggctctata
    33841 atgtcg
//
LOCUS       DAPIDM010000013        33596 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000013 DAPIDM010000000
VERSION     DAPIDM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.013"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(110..1351)
                     /locus_tag="RJE42_000551"
     CDS             complement(110..1351)
                     /locus_tag="RJE42_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV5876004.1"
                     /translation="MNYGQFEIESTIIATLLKQPDVLEKIRVKDYMFTNEKFKTFFNY
                     VMDVGKIDHQEIYLKATKDKEFLDADTITKLYNSDFIGYGFFERYQQELLESYQLNKA
                     NELVTEFKQQPTNQNFNNLIDELKDLKTITNRKEDGTKKFVEEFVDELYSDSPKKQIK
                     TGYKLMDYKIGGLEPSQLIVIAARPSVGKTGFALNMMLNIAQNGYKTSFFSLETTGTS
                     VLKRMLSTITGIELTKIKEIRNLTPDDLTKLTNAMDKIMKLGIDISDKSNITPQDVRA
                     QAMRHSDRQQVIFIDYLQLMDTDAKVDRRVAVEKISRDLKIIANETGAIIVLLSQLNR
                     GVESRQDKRPMLSDMKESGGIEADASLAMLLYRDDYYNRDEDDSITGKSIVECNIAKN
                     KDGETGIIEFEYYKKTQRFFT"
     gene            complement(1348..1704)
                     /locus_tag="RJE42_000552"
     CDS             complement(1348..1704)
                     /locus_tag="RJE42_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876005.1"
                     /translation="MPMSKTQALEIIKKVRYVYNIDFDKPKLEMWIDVLSQNGDYQPT
                     VKAVDVYINSNNPYPPNLPAIMRKEPKKVSIEPVDNETATHQWKMQNDPEYVRQRKIA
                     LDKFMNKLAEFGGENE"
     gene            complement(1704..2459)
                     /locus_tag="RJE42_000553"
     CDS             complement(1704..2459)
                     /locus_tag="RJE42_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDV5876006.1"
                     /translation="MNKLLIDDYPIQVLPKLAELIGLNEAIVLQQIHYWLNNSKHKYD
                     GKTWIFNSYPEWQKQFPFWSERTIKRTFGSLEKQNLLHVGNYNKAGFDRTKWYSINYE
                     TLNKLVARPSGQNGPTMRTNWHDARGQNDPTNTIDYTETNKHRETDDVSKSFKYISTN
                     LEIIQNPLKAEQLEHEIKSFKQDQFEIVKVATDYCKENNKGLNYLLTVLKNWNKEGVS
                     DKESAENKLKPRNSKKETTDDVIAQMEKELSDD"
     gene            complement(2452..3126)
                     /locus_tag="RJE42_000554"
     CDS             complement(2452..3126)
                     /locus_tag="RJE42_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876007.1"
                     /translation="MPIITSYITQDDGTTTVVISGVELGNKETLLLDNGFDVEVDVSV
                     IDPFQITGQQRKLIFALCNDIEAHTGQPRDYMRQMFQDYVKFLYGYEERISLSNCSRT
                     IAKQIIEAMFEWIFTNAIPLNYKTSKLMKEDKNYLYWATVTRHCIICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCREHHNQQHAIGVKSFDDKYQLHDSWIKVDERLNKML
                     KGEKNE"
     gene            complement(3140..3565)
                     /gene="ssb"
                     /locus_tag="RJE42_000555"
     CDS             complement(3140..3565)
                     /gene="ssb"
                     /locus_tag="RJE42_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV5876008.1"
                     /translation="MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGER
                     EADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTRSYENKVGQRVFVTEVVADSVQF
                     LEPKNNNQQPNNNYHQQGQTQTGNNPFDNTEEDFSDLPF"
     gene            complement(3565..4188)
                     /locus_tag="RJE42_000556"
     CDS             complement(3565..4188)
                     /locus_tag="RJE42_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDV5876009.1"
                     /translation="MTEQTLFEQLNSKNVNDHTEQKNGLTYLAWSYAHQELKKIDPNY
                     TVKVHEFPHPDINTENYFVPYLATPEGYFVQVSVTVKDSTETEWLPVLDFRNKSLAKG
                     SATTFDINKAQKRCFVKASALHGLGLYIYNGEELPSASDNDITELEERINQFVNLSQE
                     KGRDATIDKTMRWLKISNINKLSQKQIAEAHQKLDAGLKQLDSEEKQ"
     gene            complement(4181..4402)
                     /locus_tag="RJE42_000557"
     CDS             complement(4181..4402)
                     /locus_tag="RJE42_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDV5876010.1"
                     /translation="MKQTVTYLIKHKDENLFITNRPTEVNDTVKYSTDMRDAREFDGL
                     DKTVIDMSKHKAIKKTVTETIEYEEVEHD"
     gene            complement(4412..4672)
                     /locus_tag="RJE42_000558"
     CDS             complement(4412..4672)
                     /locus_tag="RJE42_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDV5876011.1"
                     /translation="MYYKFGEIKNKIINFKGFEFKVSVMKKHDGISIQIKDMNNVPLK
                     SFHVIDLSELYIAIDAMHDVVNEWIEENTDDYDRLINLVMRW"
     gene            complement(4677..4979)
                     /locus_tag="RJE42_000559"
     CDS             complement(4677..4979)
                     /locus_tag="RJE42_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDV5876012.1"
                     /translation="MTKNYKDMTQDEIRDLLSKKSGELYELAKEIKGESKFDILFFSA
                     IGVSDGDFIKSSSSALGNAFNLAELLDNATNFDDVINAIQKRKLQKFLAIDNNKED"
     gene            complement(5074..5235)
                     /locus_tag="RJE42_000560"
     CDS             complement(5074..5235)
                     /locus_tag="RJE42_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDV5876013.1"
                     /translation="MSDTYKSYLIAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(5247..5510)
                     /locus_tag="RJE42_000561"
     CDS             complement(5247..5510)
                     /locus_tag="RJE42_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5876014.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKDYREDNLGVENLYIDYSATGTLINISKLEEYLIRKHKKWY"
     gene            complement(5537..5752)
                     /locus_tag="RJE42_000562"
     CDS             complement(5537..5752)
                     /locus_tag="RJE42_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876015.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            5807..6172
                     /locus_tag="RJE42_000563"
     CDS             5807..6172
                     /locus_tag="RJE42_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876016.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            complement(6141..6386)
                     /locus_tag="RJE42_000564"
     CDS             complement(6141..6386)
                     /locus_tag="RJE42_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDV5876017.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            6442..6651
                     /locus_tag="RJE42_000565"
     CDS             6442..6651
                     /locus_tag="RJE42_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876018.1"
                     /translation="MKACLYLSNDKFVEIDNLEKVIKSGHRGTVEISKEKIKSSLFTN
                     GSYTFVGDKIVAIASAKIEFIEFID"
     gene            complement(6641..6784)
                     /locus_tag="RJE42_000566"
     CDS             complement(6641..6784)
                     /locus_tag="RJE42_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876019.1"
                     /translation="MLQKFRIAKEKSKLKLNLLKHANSNLETRNNPELLRAVAELLKE
                     INR"
     gene            complement(6824..7048)
                     /locus_tag="RJE42_000567"
     CDS             complement(6824..7048)
                     /locus_tag="RJE42_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876020.1"
                     /translation="MTPEQKEKLNNIVLTLYAVKENKSQTYTHKDTLTVTYAGEIEHT
                     YEVDREKHLESMIEWAIDQIEQHFDLDGEE"
     gene            complement(7049..7828)
                     /locus_tag="RJE42_000568"
     CDS             complement(7049..7828)
                     /locus_tag="RJE42_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDV5876021.1"
                     /translation="MQALQTFNFEELPVRTLTVDNEPYFVGKDVAEILGYSNTRDALS
                     KHVDEDDKEILTSRNTTLENLPNRGLTAVNESGLYSLIFSSKLESAKRFKRWVTSDVL
                     PAIRKHGIYATDSVIENTLNNPDYIINILTEYKKEKEQNLLLQQEIGELKPKADYVDE
                     ILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTESDTI
                     AIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA"
     gene            complement(7854..8081)
                     /locus_tag="RJE42_000569"
     CDS             complement(7854..8081)
                     /locus_tag="RJE42_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BetR domain protein"
                     /protein_id="HDV5876022.1"
                     /translation="MNKAKLYSALAMKEMHVNDFLKELNNHGLKLSKSAYYSRIRGEQ
                     EFDIKEIKTIVKVLNLTREEMNDIFFGELVS"
     gene            8253..8867
                     /locus_tag="RJE42_000570"
     CDS             8253..8867
                     /locus_tag="RJE42_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5876023.1"
                     /translation="MKPDIKSRRKELNLTLEQVGDLVGVGKSTVRKWETGDIENMKRD
                     KIVKLSKALRVSPSYIMGIEDEKPQLETIPVKKIPVVSKISAGMPIYTEENLIDYIYF
                     ATKNLNSNKEEFGLQVSGDSMDKLFQDGDVVVVEKDSTVENGQLGVVLVNGYNGTVKR
                     IRYNNDQIILIPESNNPSHYPQVYGKDDEVKIVGRVVASQKLFK"
     gene            8883..9803
                     /locus_tag="RJE42_000571"
     CDS             8883..9803
                     /locus_tag="RJE42_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV5876024.1"
                     /translation="MDFVAIDVETANEQRSSICSIALLKFQDNKMRDAIYTHINPETY
                     FSSINTSIHGISEEDVMDSPKMYQMHEAIIRFLDNHIIVAHNTSFDMYALCDSFKKYN
                     LPLPSNQYFCTYRLSKDLYNLPSYRLQDLAEHFDIKNEEYHNAFYDAKVCAKIAYNIL
                     KDQDANLNDLVENQNFKYGVFGQNGFIKKSANKKIELVYNEELNDKNHILYGKHVCFT
                     GALKGFTRNEIAQTVTNIGSVYDSSVKKSTNYLVVGNLENLEKTHNYTKSSKIKKAEK
                     LSSEGQDIEILSEMDFLKLLTTFTSVETKT"
     gene            9921..11579
                     /locus_tag="RJE42_000572"
     CDS             9921..11579
                     /locus_tag="RJE42_000572"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDV5876025.1"
                     /translation="MSDYKLSPIVKWAGGKTQLLDAINALVPNDFAIYHEPFLGGGAT
                     LLSNQPKNAIINDLNYELMTTYNVIKHDITPLIKELKDMIKQHNTNNAKDFYMTVREQ
                     EILNLNDIEIAARFLYLNKTGFNGLYRVNSQGKFNVPFNKKDMIKNSTVFSETNLRNL
                     NKYFNENNIIILNEDFNEALKKVKENDFVFIDSPYDEAYTSYQKGGFHEKEHKELAER
                     LIELDKKGVKWIVTNHNTKLIQFLYNQFDFYEIPVNRFINSDAQKRSNATNEVLILNY
                     KPTKRQLKEFERAKFYKQLKPTSFVLKEYVKWEKLQENVREYELQLNDLNVLMASDEF
                     EFEEKFERLYSQRAESFDILPLFISSRNKQIEYWSSDGEAKKYGFDKKETVFDFLVES
                     GLRENLFMNNRYKNVLDYILGLEVGLSSNDKKNYTGTWMMNQIANLLKENDITFRKEV
                     PYKEIIDANRIKDKTFDFVFNKDDVTYCLEVNFFNTSGSKINSEAERFIELNKELQNY
                     EDIEFIWVTDGIGLKKNQTSINKAMKSIRNLYNLTTFDEFLKEL"
     gene            11638..12843
                     /locus_tag="RJE42_000573"
     CDS             11638..12843
                     /locus_tag="RJE42_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV5876026.1"
                     /translation="MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEDKIKEKLNNQSSSILKTLTFHTLLDEWLEYHTKTSGFKVTTLDNLKTRIK
                     NIKKNSSQNLLLNKIDTKYMQTFINELSNIYSENQVKRQLGHMKEAIKYAVKFYNYPN
                     EHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAG
                     AVEVQALTGMRIGELLALQVKDVDLKNKTIDINGTIHRIKCNAGFGHKDTTKTAGSRR
                     KIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAE
                     SLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHTDEKTTIKVYTHVTEKMDREL
                     EQKLEKLVY"
     gene            complement(12937..14871)
                     /gene="lip2"
                     /locus_tag="RJE42_000574"
                     /pseudo
     CDS             complement(12937..14871)
                     /gene="lip2"
                     /locus_tag="RJE42_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
     gene            15288..16601
                     /locus_tag="RJE42_000575"
     CDS             15288..16601
                     /locus_tag="RJE42_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDV5876027.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAIIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFGITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            complement(16731..17399)
                     /locus_tag="RJE42_000576"
     CDS             complement(16731..17399)
                     /locus_tag="RJE42_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV5876028.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            17539..18339
                     /locus_tag="RJE42_000577"
     CDS             17539..18339
                     /locus_tag="RJE42_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV5876029.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     INNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            complement(18616..19476)
                     /locus_tag="RJE42_000578"
     CDS             complement(18616..19476)
                     /locus_tag="RJE42_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV5876030.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYHLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            19634..20515
                     /locus_tag="RJE42_000579"
     CDS             19634..20515
                     /locus_tag="RJE42_000579"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV5876031.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLIISGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            20555..22087
                     /locus_tag="RJE42_000580"
     CDS             20555..22087
                     /locus_tag="RJE42_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDV5876032.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLLIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARLVIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPPKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(22193..23413)
                     /locus_tag="RJE42_000581"
     CDS             complement(22193..23413)
                     /locus_tag="RJE42_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV5876033.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGILNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPKL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(23424..24347)
                     /locus_tag="RJE42_000582"
     CDS             complement(23424..24347)
                     /locus_tag="RJE42_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV5876034.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            complement(24322..25440)
                     /locus_tag="RJE42_000583"
     CDS             complement(24322..25440)
                     /locus_tag="RJE42_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV5876035.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            complement(25783..26814)
                     /locus_tag="RJE42_000584"
     CDS             complement(25783..26814)
                     /locus_tag="RJE42_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV5876036.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGLVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            complement(27022..27699)
                     /locus_tag="RJE42_000585"
     CDS             complement(27022..27699)
                     /locus_tag="RJE42_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876037.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(27712..28761)
                     /locus_tag="RJE42_000586"
     CDS             complement(27712..28761)
                     /locus_tag="RJE42_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876038.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            complement(29010..29900)
                     /locus_tag="RJE42_000587"
     CDS             complement(29010..29900)
                     /locus_tag="RJE42_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDV5876039.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     MPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQIVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            30484..31794
                     /gene="brnQ"
                     /locus_tag="RJE42_000588"
     CDS             30484..31794
                     /gene="brnQ"
                     /locus_tag="RJE42_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDV5876040.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     ITGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDHLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFIAGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFILLAIIGFIIDVVIRRPKQATTK"
     gene            32030..32854
                     /locus_tag="RJE42_000589"
     CDS             32030..32854
                     /locus_tag="RJE42_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV5876041.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(33070..33468)
                     /locus_tag="RJE42_000590"
     CDS             complement(33070..33468)
                     /locus_tag="RJE42_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDV5876042.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLFMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
BASE COUNT        10364 a         6021 c         5324 g        11887 t
ORIGIN      
        1 aatctgttga ctgcccaacc agtctcgata tatacatttg ctacaatcgg gtcgctttgc
       61 tttgtctctt catacatcga tttcaataag cttttgaatt gcattatatt catgtgaaaa
      121 acctctgagt cttcttgtaa tactcaaatt caattattcc ggtttcgccg tctttgtttt
      181 tggctatgtt acattcaaca atagatttgc cagtgatact gtcatcttcg tcacggttat
      241 aataatcatc acggtaaagt agcatcgcta aactcgcatc tgcttctatt ccgcctgatt
      301 ctttcatgtc cgatagcatt ggtcttttat cctgtctaga ctcgacacca cgattcagtt
      361 gtgaaagtag tacgatgatt gcgcctgtct cgttagcgat tatctttaag tcacgtgata
      421 tcttttctac tgctacacgt ctatcaactt tcgcatcagt atccatcagt tgaagataat
      481 ctataaaaat aacttgttgc ctgtctgaat gcctcattgc ttgcgctcgc acatcttgcg
      541 gtgtgatatt acttttatca gaaatatcga tgcctaattt catgatttta tccatcgcat
      601 tcgttaactt tgttaagtca tccggcgtta agttcctgat ttcttttatc tttgttaact
      661 caataccagt aattgttgat aacatacgtt tcaatactga tgtgccagtt gtttcgagac
      721 taaagaaaga tgttttgtat ccattttgtg ctatgttcag catcatgttt aatgcaaaac
      781 ctgtcttacc cactgaggga cgcgctgcga tgacgattaa ttgcgacggc tccaatcccc
      841 ctattttgta atccatgagc ttataacccg tcttaatttg cttcttaggg ctatcgctgt
      901 ataactcatc gacaaactcc tcaacaaact tcttggttcc gtcttctttt ctgttagtaa
      961 ttgtttttaa atccttgagt tcatcaatca agttattaaa gttttggttc gtaggttgtt
     1021 gtttgaactc agttactaat tcgttagctt tgttgagctg ataactttcc aataattctt
     1081 gttgataacg ttcaaagaag ccgtatccaa tgaaatcgga gttgtaaagt ttagttatag
     1141 tatctgcatc taaaaattct ttatctttag ttgcttttaa atagatttct tgatgatcta
     1201 tctttccgac gtccattaca taattgaaaa aggttttaaa cttttcgttc gtaaacatgt
     1261 aatctttaac tcttatcttt tctaatacgt ccggttgttt aagtagcgta gcgattattg
     1321 tactttcaat ttcgaattgt ccgtaattca ttcgttttcg cccccaaatt ctgccaactt
     1381 attcatgaac ttatctagcg ctatttttct ttgtctgaca tattcggggt cattctgcat
     1441 tttccattgg tgtgtagcgg tttcgttatc tactggctcg atagatactt ttttaggttc
     1501 cttacgcatg attgctggta agttaggcgg gtacgggttg ttactgttga tataaacatc
     1561 taccgctttt acagttggtt gataatctcc attttgactt aatacatcaa tccacatttc
     1621 taacttcggt ttatcaaaat caatgttgta tacgtaccta acttttttaa taatttctaa
     1681 tgcttgtgtt ttgctcatcg gcattagtca tcactcaatt ctttttccat ttgtgctatg
     1741 acatcatcag tagtttcttt tttagagtta cgaggtttca atttgttttc agcactttct
     1801 ttatctgaaa cgccttcttt attccagttc tttaatacag ttagtaagta attcagacct
     1861 ttgttgtttt ctttgcagta atcggtagcg acttttacta tttcgaactg atcttgctta
     1921 aatgatttaa tttcgtgttc taactgttct gcttttaaag ggttttgtat aatttctaaa
     1981 ttggtactaa tatacttaaa tgactttgag acgtcgtctg tctctctatg tttgttagtc
     2041 tctgtgtagt ctatggtatt ggtcgggtca ttttgtcctc ttgcatcgtg ccaatttgtc
     2101 ctcatcgtcg ggccattttg tcccgatggt cgtgccacta gtttgtttaa tgtttcataa
     2161 ttgattgaat accattttgt acggtcaaat ccagccttgt tgtagttacc tacatgcaat
     2221 aaattttgtt tttctaaact cccaaatgtc ctttttatag ttctctcgct ccaaaatgga
     2281 aattgttttt gccattctgg ataagaatta aaaatccaag ttttaccatc gtacttatgt
     2341 tttgagttgt ttaaccaata atgaatttgt tgcaatacta ttgcttcgtt taatcctatt
     2401 aattcagcta atttcggtaa tacttgtatc ggatagtcat ctattagtaa cttattcatt
     2461 tttctctcct ttcagcattt tgttgagcct ctcatcaact tttatccacg agtcatgcaa
     2521 ttgatattta tcatcaaatg acttaacacc aatcgcatgt tgctggttat gatgttcgcg
     2581 acataacgct aatacatgtt tgtcgtagtg attcatcttg tttctgttca tgcctcttcc
     2641 aactgcttca taatgcgcta ggtcagcgtg aggctttccg catataatgc aatgacgcgt
     2701 aacagttgcc caataaagat aatttttatc ttctttcatc aatttgcttg ttttataatt
     2761 taacggaatc gcatttgtaa aaatccactc aaacatcgct tctataattt gcttagctat
     2821 agttcgagaa caatttgata aagatatacg ttcttcatag ccatacagaa acttcacata
     2881 atcttggaac atttgcctca tataatctcg aggttgtcct gtatgagctt ctatatcgtt
     2941 acacaatgcg aatattaact tacgttgttg tccagtaatt tgaaacggat ctatgacgct
     3001 tacatcgact tccacatcaa atccgttatc aagtagtaat gtttctttat tgcctaattc
     3061 gacacccgag atgacaactg ttgttgtgcc gtcatcttga gtgatataac tagtaattat
     3121 tggcatctaa tcattccaat cagaacggta agtcagaaaa gtcttcttcg gtattgtcga
     3181 acggattatt accagtttga gtttgtcctt gttgatgata attgttgttt ggttgttggt
     3241 tgttattctt cggttctaag aattgaacac tgtccgctac tacttctgtc acaaatacac
     3301 gttgcccgac tttattttcg tagctacgtg tttgtagtcg cccgtctaca cctgccagcg
     3361 accctttaga aaggtagttt ttaacatttt cagcttgttt cttgaacact actacgttta
     3421 taaaatctgc ttcacgctcg ccttgagcat tcgtgaatgt tctgtttact gccaatgtga
     3481 atgtacctac atttacgcca tttggcgcac ttcttaattc tgggtctttt gttaagcgtc
     3541 ctactaatac tgctctgttt aacattattg tttctcctca ctatccaatt gttttaatcc
     3601 cgcatctaat ttttggtgtg cttctgcgat ttgtttttga cttaatttat taatgttaga
     3661 tatttttagc catctcatcg ttttatcgat agttgcatct cgcccttttt cttgagataa
     3721 gttcacgaac tgattgatac gctcttctaa ttctgtaata tcgttgtcac ttgcacttgg
     3781 tagttcctcg ccgttgtaga tatataagcc taaaccgtgt aaagccgaag ctttaacaaa
     3841 acatcgtttt tgcgctttgt taatatcgaa agttgttgca ctacctttag caagcgattt
     3901 atttctaaag tccaatactg gaagccactc agtctctgta ctatctttca cagtcacaga
     3961 tacctgtaca aaatagcctt ctggtgtagc caaataaggt acaaaataat tttctgtgtt
     4021 aatatctgga tgtggaaact cgtgtacttt tactgtgtag tttgggtcaa tctttttcag
     4081 ctcttggtgt gcatatgacc atgctagata agttaatcca tttttttgtt ctgtatgatc
     4141 attcacgttt ttactgttca actgttcaaa taatgtttgt tcagtcatgt tctacctcct
     4201 catactcaat tgtttctgtc actgttttct tgattgcttt gtgcttagac atatcaataa
     4261 cagttttgtc tagtccgtcg aattctcttg cgtctcgcat atcagttgaa tacttcactg
     4321 tgtcgttcac ttcggttggt cggtttgtaa taaatagatt ttcatcttta tgcttgatta
     4381 gataagttac agtctgcttc atagcgacct cctaccatct catgactaag ttaattagtc
     4441 tgtcgtaatc atctgtattc tcttcaatcc attcgtttac aacgtcgtgc attgcatcta
     4501 ttgcaatata tagttcgctt aaatctatga catgaaacga tttaagtgga acattattca
     4561 tatccttgat ttgtatactg ataccgtcat gcttcttcat tacagacact ttaaattcga
     4621 accctttaaa gtttataatt ttgtttttta tctcaccaaa tttgtaatac attgttttag
     4681 tcctccttgt tgttatctat agcaagaaat ttttgtagtt tacgtttttg aatggcgtta
     4741 atgacatcgt cgaaattagt agcattatcc aataattcag caagattaaa agcattgcca
     4801 agcgcagaac ttgaactttt tatgaaatct ccgtcgctaa ctcctattgc tgagaaaaac
     4861 aaaatatcaa atttactttc tcctttaatt tctttcgcta attcatacaa ttctccgctt
     4921 tttttagata ataagtctct tatttcgtcc tgcgtcatgt ctttataatt tttagtcata
     4981 gttgacttcc tccttgtttc gttttatatt gaacatgaat taattttgtt aatcgtttgt
     5041 cactgttact tgttggcgca agtagcagtt tttttattct tcataaaagt attccttata
     5101 aaatatgaat gtcgctatgc ttgcgaatcc tgcaattgac catgctgtag tgaagtatag
     5161 aaacggcatg agtacaatcg ctaagactgt gaagcacagt actgctatta aatagctttt
     5221 atatgtgtcg ctcatttaat atcctcctaa taccattttt tatgctttct gatcaaatac
     5281 tcttccaatt tagaaatatt aatcaatgtt cccgttgctg aataatcaat gtataaattt
     5341 tctacaccta aattatcttc acggtaatct ttcaaccagt tgtatactgt acttctacat
     5401 actccaaaca attgatggat ttgtgtaggt gttgcgtata actttttcac aaatttttct
     5461 tcgcctctat atgtgttttc tggtgttggt ggtactatga tttttggcat ttctatcttt
     5521 ccttccgtgt ataatgttaa agtttgttat tattcgccct gtattgaagt tctctatcta
     5581 atgcatagaa aactttgttt atttctaagt agctgtaatc acttttttta ataagctcta
     5641 atatttccgc tcctaagtta cgttcctttt ccgttaaata ggatgaagaa gcatcagctt
     5701 tgctagaaac ttgtgggacg cctatacgca atccttctga tcttgtgttc atttgtttat
     5761 gctcctttcg tgtataatgt tgttatcaac ctaaggaggt gataacatgc ccttgatatc
     5821 tgatgaattt gatacactta ctaaagacca acaatatatc ttgtccgtac tctacaaaga
     5881 ttatttagaa tgcgtaaagt taggttcggt taaattaacc tgcaataatt ttggaagtgc
     5941 taaagatata catacaaagt attttcaaaa actacatttc gaagatgtaa aatacgattt
     6001 aaataaactt aaaaactctg ggttcctaaa cggcgtgtat gctagtaaca ctatttatca
     6061 tgtaacaatt tcagacaaga ctgttgttta ctttgaaaat gagtttaaaa acaatttaaa
     6121 aagtatcatt gatagcattt ctaaaattgc ttcaataatt cctggtctct agttgggttt
     6181 ataacttccc aatcatttgc catgaggtca tcggctgaag gttgccaata tctgataagg
     6241 tttgtcccat cgctatttga aatgatgcat tgtaaaaaac tatcatttgt tggtaatatc
     6301 ttagttcgat gactttcctt ccaatctttc cgtgtcatag agacaagatt ttttgtagct
     6361 atcttagttg cttcttgaat gttcatttgt tattcctcct ttcgtgtata atgttgttat
     6421 caacctaagg aggtgataag tatgaaagct tgtttatatc tttctaatga taaatttgtt
     6481 gaaatcgata atttagaaaa agtgataaag tcaggtcatc gcggaactgt tgaaatatca
     6541 aaagaaaaaa ttaaaagttc cttgttcact aatggctcat atacttttgt tggagacaaa
     6601 atagtagcta tcgcttcagc taaaatcgaa ttcatagaat ttatcgatta atctctttaa
     6661 gcaactctgc aactgctcgc aacagttcag ggttgtttct tgtttctaaa ttactgtttg
     6721 catgttttag taaattgagt tttaatttac ttttttcttt agcgattcta aatttttgta
     6781 acatttgttg ttcctccttt taagatgttt gttcaattgt gtgttattct tctccgtcta
     6841 aatcaaagtg ctgttcgatt tggtcaattg cccactcaat cattgattca aggtgtttct
     6901 ctctgtcgac ttcgtaagtg tgctcaatct cgcctgcata tgtcacagta agagtatctt
     6961 tgtgtgtgta tgtttgactt ttgttttctt taactgcata aagtgttaat actatattgt
     7021 ttagcttttc tttttgttct ggtgtcattt acgctccccc taaattagct tcataaccga
     7081 attcagtcat gatttcatgt attttcaatc tgcctttttg tgtccatcta gtttgtaaaa
     7141 ctgtgtcttc tctaccgtca gagcgtacaa ttgctatagt gtctgattct gtgtaactct
     7201 tgcccatgtg ttctgagtaa agcacccact gtttatttac ttttcgttgt aatctagctt
     7261 cgtgtagtag tttgtttaac ttttgtgctg atataccgta gtctgccgcg atttgagttg
     7321 tggctaatgt gccagttgac tttaagattt catctacata gtctgctttg ggttttagtt
     7381 ctccgatttc ttgttgtaaa agtaagtttt gctctttttc tttcttatac tcagtaagaa
     7441 tgttaatgat gtaatctgga ttgttcagcg tgttctcaat tacactgtct gttgcgtaga
     7501 taccgtgttt gcgaatggct ggtaggacgt ctgatgttac ccagcgtttg aatcgttttg
     7561 ctgactctag ttttgatgag aagattaagc tgtataaacc tgattcgttg actgcagtaa
     7621 gtcctcgatt tggcaaattt tctaaagtcg tgtttcgcga cgttagaatt tccttatcgt
     7681 cttcatcaac gtgtttactt aatgcgtcac gcgtattcga gtatcctaag atttctgcca
     7741 catctttacc tacaaaatat ggttcgttat ctactgttaa tgttcttact ggtaattctt
     7801 cgaaattaaa tgtttgtaat gcttgcattt gtttatgcct cctcaagtgt cttttaggac
     7861 accaattctc caaaaaaaat atcgttcatt tcttctctgg ttaaattaag aactttgact
     7921 atcgtcttaa tttctttgat gtcaaattct tgttctcctc taattctact ataataagcg
     7981 cttttagaaa gttttaaacc atgattattc aattctttta aaaaatcatt tacatgcatc
     8041 tctttcatcg ctaaagcaga ataaagtttc gctttgttca tttttcaaca cctccgtgtc
     8101 ttttaggata cttcaatgat acatgctaat atcaatctag tcaacacaaa agtgtctaaa
     8161 aatacatttt tgtttttatt tcctatataa gtgttgcttt taagaaactt tagtgttata
     8221 ttcagtacat acctattagg aggaaattta aaatgaaacc agacattaaa agcagacgta
     8281 aagaattgaa tttaactcta gaacaagttg gagatttagt tggtgtaggt aaatccacag
     8341 taagaaaatg ggaaactggc gatatcgaaa atatgaagag agataaaata gtaaaattat
     8401 ctaaagcatt aagagtatct ccatcatata tcatgggtat tgaagatgag aaacctcaac
     8461 ttgaaaccat accagttaaa aagataccgg ttgtatcaaa gatatctgcg ggaatgccca
     8521 tatatacaga agagaattta atcgactaca tatactttgc tactaaaaat ttgaactcta
     8581 ataaagaaga gttcggattg caagtgtctg gggatagtat ggataaactc tttcaagacg
     8641 gagacgttgt agttgttgaa aaggattcga ctgttgagaa tggccaatta ggtgttgtat
     8701 tagtcaatgg atacaacggt actgtcaaaa gaatacgtta caacaacgac caaattattt
     8761 taattcctga atcaaacaat cctagtcact acccacaagt atatggaaaa gatgacgagg
     8821 ttaagattgt aggtagagtt gtagcaagtc aaaaactatt caaataaaag gggctgatta
     8881 taatggattt tgtagcgatc gatgtcgaaa cagctaacga acaacgcagt tctatttgtt
     8941 caatagcact attaaaattt caagacaaca aaatgcgtga tgctatttat actcacataa
     9001 atcctgaaac ttatttttct tctataaaca cttctataca tggaatatcc gaagaagacg
     9061 taatggattc ccctaaaatg tatcaaatgc atgaagctat tattagattc ttagataacc
     9121 atataatagt agcccataat acatctttcg atatgtatgc tctatgtgat tctttcaaaa
     9181 aatacaatct acctttacct agcaatcaat atttttgtac ttatcgatta agtaaagatt
     9241 tatataattt accttcatat agactccaag atttagctga acattttgac attaaaaacg
     9301 aagaatatca caatgctttt tatgatgcta aagtgtgtgc caaaattgca tataacatat
     9361 taaaagacca agatgcaaac ttgaacgatt tagtcgaaaa ccaaaatttt aagtacggtg
     9421 tgtttggtca gaatggattc ataaaaaaat cggctaataa gaaaatagag ctagtataca
     9481 atgaagagtt aaacgataaa aatcatatat tatatggtaa acatgtttgt tttaccggag
     9541 cgttaaaagg tttcacacgg aacgaaatag cccaaactgt aactaatatt ggttctgttt
     9601 acgattcctc tgtaaaaaag tcaactaact acctcgttgt cggtaattta gaaaacctag
     9661 aaaaaactca taactatacc aagtcaagta agattaaaaa agcagaaaaa ttatcaagtg
     9721 aaggacaaga cattgaaatt ttaagtgaaa tggatttctt aaaattatta actaccttca
     9781 cttctgtaga aacgaaaacg tgatgtcggt tgataatgtt ttattgtgat taacagcgcc
     9841 tgtgtggcga tttaataaat tcatatgaat caatgccaat gtatactata ataaataagt
     9901 atttgaaagg agatgaaatg atgtcagact ataaattgtc accaattgtc aaatgggctg
     9961 gtggaaaaac acaattatta gatgcgatta atgcactcgt accaaacgat tttgctatct
    10021 atcatgaacc gtttttaggc ggaggtgcaa cattattatc taatcaacct aaaaacgcta
    10081 taattaacga tcttaattat gaattaatga caacatacaa tgttatcaaa catgacatta
    10141 cacctttaat taaagaactt aaagatatga taaaacagca caacactaat aatgctaaag
    10201 atttttatat gacagtaaga gagcaagaaa tactcaattt aaatgatata gaaatagccg
    10261 caagattctt ataccttaac aaaactggtt ttaatggttt atatagagta aatagccaag
    10321 gtaaatttaa tgtacctttt aataaaaaag acatgataaa aaactctact gtattttctg
    10381 aaacaaactt aagaaattta aataaatatt ttaacgaaaa caacatcata atattaaacg
    10441 aagactttaa cgaagcttta aaaaaagtta aagaaaatga ttttgtcttt atcgatagcc
    10501 cgtatgacga agcatacact agttatcaaa aaggtgggtt tcatgaaaaa gagcataaag
    10561 aactagcgga aaggttaatt gaattagata aaaaaggcgt taaatggatt gtgacaaatc
    10621 ataatactaa gttaatacaa tttttgtata accaatttga cttttatgaa attccagtaa
    10681 acagattcat taattccgac gctcaaaaga gaagcaacgc aactaatgaa gtcttaatat
    10741 taaattacaa accaaccaag agacaactaa aagaatttga aagagcaaag ttttataaac
    10801 aattaaaacc tacttctttt gtactcaaag aatatgtaaa atgggaaaaa ttgcaagaaa
    10861 atgtaagaga gtatgaatta caattaaatg atttaaatgt attaatggca agtgacgaat
    10921 ttgaattcga agaaaaattc gaacgtcttt attcacaaag agccgaatcg tttgatattt
    10981 taccgctgtt catatcatcc cgtaataaac aaattgaata ttggtcaagt gatggagagg
    11041 ctaaaaaata tggttttgat aaaaaagaaa ctgttttcga ttttctagtc gaaagcggtt
    11101 taagagaaaa cttgtttatg aataatagat ataaaaatgt cttagactat atattaggtc
    11161 tcgaagttgg attgagtagt aatgataaaa agaattacac tggaacatgg atgatgaatc
    11221 aaatagcaaa tttacttaaa gaaaacgata tcacattcag aaaagaagtg ccttacaagg
    11281 aaataatcga tgcgaataga attaaagata aaacttttga ctttgttttt aataaagacg
    11341 atgttactta ttgcttggaa gttaactttt ttaatacgtc tggtagcaaa ataaattccg
    11401 aagcagaaag atttatagaa ttgaataaag aattgcaaaa ttatgaagat atagagttta
    11461 tatgggtgac tgacggaata ggactcaaaa aaaatcaaac ttctataaat aaagctatga
    11521 aatctattag aaatttatat aaccttacaa cattcgatga atttctaaaa gaattataat
    11581 aacgggtagc ccgcctaccc ttattatttt tttgccaatt ttgaggaggg atgtaaaatg
    11641 tggtttgaaa aatttaaaaa taagaacaat gaaacgaagt atagatacta cgagaaatac
    11701 aaagatccgt atacagataa atggaaacgt gtaagtgttg tcttgaataa gaatacaaag
    11761 caatcgcaaa aagaggcaat gtttcgatta gaagataaga taaaagaaaa attgaataac
    11821 cagtcatcaa gcattttaaa aactttgact tttcatacac tattagatga atggcttgaa
    11881 tatcatacaa aaacatctgg ctttaaagta acgacgcttg ataatttgaa aacaagaatc
    11941 aaaaacatca aaaagaacag ttctcaaaat ttacttttaa acaaaattga tacaaagtac
    12001 atgcaaacat ttattaacga attatcaaac atatattctg aaaatcaggt aaagcgtcaa
    12061 cttggacata tgaaagaagc tattaaatac gccgttaaat tttacaatta tccaaacgaa
    12121 cacatattaa atagcgtcac actaccaaag aagagtaaga cgatagaaga tatagaaaaa
    12181 gaagaagcga aaatgtacaa ctatttagag atggaacagg taatacagat acgcgatttt
    12241 atactgaacg ataataacat gcagtataga gctcgtattt tagttgctgg ggctgtagaa
    12301 gttcaagctt taacaggtat gcgcataggt gagttattag cgctccaagt taaagatgtt
    12361 gacctcaaaa ataaaacgat cgatattaat ggcactattc acagaatcaa atgtaatgct
    12421 ggatttggtc acaaagatac tactaagacc gcaggttcaa gaagaaaaat cgccatcaat
    12481 tcaaggatag caaatgtatt gaaaaaaata atgttagaaa ataaaaagat gcaacaatgg
    12541 gaaccaagct atgttgatag agggtttata ttcacaactt gccaaggaaa tcctatgcaa
    12601 ggcagtagga taaacaaacg attgtcctca gctgcagaat cattaaatat aaataaaaaa
    12661 gttactactc acacactaag gcatacacac ataagtttat tggcggaaat gaatatatcg
    12721 ttaaaagcaa ttatgaaaag agtaggacat acagatgaaa aaacgactat aaaggtgtat
    12781 acacatgtaa cagagaaaat ggacagagag ttagagcaaa aattagaaaa acttgtgtac
    12841 taaaaggtat ctgccctttt tctgcccttt tttattttta aagacgctac aaatcctttg
    12901 taacaactga tattaaaggc tttttaacac aagttaatcc cacccttgta agattggttt
    12961 aacttgccag atacctcttc gtgtggcagg ttcattattc gtaacattaa caaatggttg
    13021 attggacgga tgtaatgacg aaatcactgg tacgacacca tcatttttac gccattcttc
    13081 tcttgcatca tgaccaataa ttctactcgt tgtatccatt aagaaaaatg tacctaaatc
    13141 aggattttca taacctaatg gaccagtatg tgaagacacg cctgtataag tcgtatacgt
    13201 aatgtttgga ttcatacttg tcatgttgtt caattttgca gagccatcta acgttaaatc
    13261 ataggcagca ttatcgtctg atgtccaaat tttgctttta ctaacgcgtt ttatatagtc
    13321 aatgtaactc tcatttggta attgtttaaa cccccattgc gttaatccta aatcgatatt
    13381 cgaatacttg ttacccataa atcgatttaa agcgaacatg atttttctaa cagcttctgt
    13441 atttccaaac ttatcagctg cttgtgaacc attatgtggt gttgctaatg ttgtgattga
    13501 tgcaaccata ttgttatgac caccagtgaa taatggtgat atttctccac catgcgcttg
    13561 atgataggca atttcttctt tgttaccatt tcttaaaaac tcttccatta aacgaattgt
    13621 ttgacccccc atactatgcc ctacaagatg taccttttta ccaggttccc aattaggcat
    13681 gattccttta tatgtcttac catagcgctc atgtccgtat ttagctgcat gtgctgcgcc
    13741 ataatctacg cgaccacctt tgatgtaata ataaagttct acagcacgat catagttgct
    13801 accaaatgca cttacacttg cttgatgtac attatatcct tgctttctca attcttcgat
    13861 aactttaaat ttatttccac cccaataatt tggatataaa gcaggtgcat tatcgcctac
    13921 taatcctaaa aatccatgta caaaaacaac aggatatttg ttaagtggtt gtacttgatt
    13981 cgctttaagt ggtcgaatct ttttagcatc tgctgctgca tttgtagtcg aacgtgtttt
    14041 aggcgcttga agcgcatctt tgattagttg tttattatca gtatttttat ctgtcggttt
    14101 ctctggggaa ttttgaccat cgatatgttt acttaaatcg ccaacttgtg gtttctgttc
    14161 actttgatga acattatcat cttgctttgc atctatgatt tgatgcgcgg gttgatgggt
    14221 gtcttgttga tttggatgtt tatccgtgga tgcatcttgt gcttttgttg atttaggtgc
    14281 agttttatca ttagatggga gtgttgttga tgttggtgct gctttatcat gatttggtat
    14341 taccttttgt gcaggaacgg atggttgtga tttatcaaca tcattcgatt gtgacgttgt
    14401 cgattgtgat tcattacgtt cagcatgatg ggtattttga acctgatcat tttgacgttc
    14461 agttttgtta gtcgcattat ttgggtgtga ctgtttagaa ccttgtcgtt cagaatcttg
    14521 ctttacttga ttatcatttt gcgttctgct tgttttacta ttttgtgttg attgatgctt
    14581 cgatgattga agcgtatctt taccttctgt cactgttcca cttttaccat tctctttatg
    14641 catatcgtct aattgctttc ctgactgcat ttgatgtgac gttaccgcat tcccttgttc
    14701 tcccggttga tttagtgttt ctttttgtgc cgctgcatta gacgttggtg ttttttccga
    14761 ggcttgtgct tcatgtgatg aaacaataaa cattgtagcc gctaacactg acaccacgcc
    14821 tattgaatac tttctaatac tatactttct ttcttcttgt cctcttaaca tataatcacc
    14881 tcaaaagtat ttttttgacc ccctcttgag aatgaaaaac aactgcacct tcaatataac
    14941 atgacatata ttattttttg attaagacta caaaattaat aaaaatttaa taatacgata
    15001 ttaaatcgat actaatcatt tacaaatatc tgtatcctca aataatttat aaatacttta
    15061 attgtggcgt tgaatcaaca caacaacatt taagataaac attttattaa gtaatatagt
    15121 ttcaatctat atataattag ttaatttgta ttggtctgaa aataattcaa actataaaat
    15181 tatagtaagt tttcagattt ttctaaatta aaataatgat tcatctcatt aaggggaaga
    15241 tgcatcatac agttttgtgt attcatttaa acaaaggggt tgctttaatg aatcaatatc
    15301 attctaatgc acaacaacca agtgcatggc gtttttttgt ctatagttta gtgggcatac
    15361 tatgtttctt tattcctttt acgattaatg gtaacaacac tattttcgtc gatcatgttc
    15421 atctagccat tcgctcaatc ataggtccac ttatgcccta tgttgcactg attatgattt
    15481 taattggtac agcgttacca atagtgagac gtacttttat gacttcaatt acaaacttgg
    15541 tcattacatt atttaaagtt gcaggtgcaa taatcggtat aatgtatgta tttaaaatcg
    15601 gtccatcaat actatttaaa gctaactatg gtccgttttt gtttgaaaaa ttaatgatgc
    15661 cattaagtat cttaattcca gtaggtgcaa ttgcgctttc tttattagtg ggctatggct
    15721 tattagaatt tgtcggtgtt tatatggagc ctattatgag acctattttt aaaacaccag
    15781 gaaaatccgc tgtcgatgca gtggcttcgt ttgtcggcag ttattcctta ggtttattga
    15841 ttactaatcg tgtctataag caagggatgt acaacaaacg agaagccaca attattgcga
    15901 ctggtttttc aacagtttca gcaactttta tgattatcgt tgctaaaact ttaggattaa
    15961 tgccacattg gaatttatac tttgggataa ctttagtcat cacatttgtc gtgactgcaa
    16021 ttactgcatg gctaccgcca atcaccaatg aatcaacaga atattataac ggacaagaag
    16081 gagaacaaga agttgctatt gaaggaagca gactgaaaac tgcatatgca gaggcgatga
    16141 aacaaaatgc attaacacca tccctcgtga agaacgtttg ggacaatttg aaagacggtt
    16201 tagaaatgac tgttggtatt ttaccttcta tattatcgat tggtttttta ggactgattg
    16261 tagcgaacta tacaccattc attgattggc ttggttatat attctatcca tttatttata
    16321 ttttcccaat tgctgatcag gctgtacttg caaaagcttc agcgatttct attgtagaaa
    16381 tgtttttacc atctttgtta gtaactaaag ctgcaatgag tactaaattt gtcgtcggtg
    16441 tagtaagcgt atcagccatt atctttttct cagcattagt gccatgtata ctagcaactg
    16501 aaattaaaat acctgtctgg aaactcatca tcatttggtt tttacgcgtg gcattgtcgc
    16561 tattaatcac catcccggtc gctttactca tttttggata attcaaattg aaaataaaat
    16621 cactatcata aacaaacaaa acgagatgaa cccattacat tcattgagtc acctaagata
    16681 attaggtacc tttaattaat gatggtcatc tcgtttttta tcattatcat ttaatcttcc
    16741 ataacttgaa caaaacgttt cgtaatttct tttggtcgtg ttatcgcacc accaacgact
    16801 gaacaatgaa cgcctaagtc catcacgcgt ttatacatat ccggtgtaat gacattacct
    16861 tccgcaatga cttttgcatc aacactttgt agtacatctt ttagaaattg aaagtcattt
    16921 tgataaagta attgcccttg cgtataacta gtatagccat gtaacgtcgt gccaatataa
    16981 tcaaagccaa gtcgtgccgc atttttagct tcttcaacgg tcgcgatatc agccatgatt
    17041 tcaacgttag gtgcatgtgt tctaatatat gatactaatt cgtctaacgt ttctttcgga
    17101 cgttgctgta acgttgcatc caatgcaatg acttcacatt ggctttctat cagttcatca
    17161 acttcttttg acgttgcagt aatgaaaaca tctgagtgat catagtcacg tttcacaatg
    17221 ccaataactg gtaaatctac cgtttcttta attgctaaaa tgtcttcctt agtatttgcg
    17281 cgaataccaa cagcaccacc ttcatacgca gctaatgcca ttttcgacat aataaaagat
    17341 gaatgcaatg gttcatctgg tagtgcctga caagatacta ttaatccatg tggtaacatt
    17401 attcctcact ccataagtag ttttttcacg taaagtcatt tacaatatat cattaagata
    17461 atttttatca atgcttttta ctatcttgaa aatatttttc atatataata gtaatcatta
    17521 agatgaaggg gtgacacaat gaaatttgag aatcgtgttc aacgttatca acatttattt
    17581 acaaaaactg ataaacgcat cgtcaattat ataagacaaa atggttatag cgatgccttt
    17641 tcaacgatta actccttagc ccatgcgatt ggtacatcac cagcaacgat gacacgcttt
    17701 agtcataagc tggattatga gaatttccaa gatttaaaat ttaatattca acaagaaatg
    17761 acagagacag ttattgaaaa tagcccaatc attcaaagaa ttcataaata tcatcaacaa
    17821 atcattcaac aaactggtga atttattaat aacgacatta tccaaacctt tatcgacaaa
    17881 cttcaatcaa gtcggcatat actctttgct ggactaggta gctctggttt atcagcgact
    17941 gaattttatt atcgtatgat tcgtatgggg ctaaaaggta atgttactac cgattcacat
    18001 ttaatgaaaa tatcggcatc cctactatct cattcggaca tgtttattgc tatgtcaaat
    18061 agtggtaata cttcagaatt aatttcagca gcggaagtcg ccaaatctca tggtgcatac
    18121 gtcgttgcca tcacaaattt cgaaggtagt aaacttacag attgtgcaga tttagtactt
    18181 ttaacaacgg atcaatcgcg taataccgac catcaattta tcaacacaca aattgcgaca
    18241 ctctttttaa tcgatatcgt gagttatcat ttattagaaa atacgaatct gagtcaaact
    18301 tatcaacata ctaaatctat tatcctagac aacaaataac tagtagtata ttcaaatcca
    18361 cctgcaactg tagacaatca tcgtgattgt cccaacgagt tgctaggtgc ttttttaatt
    18421 tcaataccaa atattttttt acatttttga gaactatact ttcaagcctc ttcaatttaa
    18481 tatgcccgtg tcacactgtt ggttgatata gtttagattg gtcctagata gggtgtgatg
    18541 tgtggaacta acaacagctc tcatctctct tatcggtttc aaattctatt ttagaatgac
    18601 tccttctttc aaccatcatt gcaaacagcc atataatgct gcatcatttt tactcttagt
    18661 cgtttgtatc ggtgcataaa catagtcttt tggtaaatag tgtgcaactt tcggctcgat
    18721 atatttaatg agattgtctc cttgttcaga tataccgcca ccaattaata taagccctgg
    18781 atcatacatg acttgtattt gggcaatccc ttctgctaca tcttctgccc actcattcaa
    18841 tatttgtttt gcaatatcat caccttcttc agctgcttca aacaatactg gcacatgtgt
    18901 gcttctcgta aatcctccgt caatcatgcg ttttttcaat gcactcgttg cagcacgttg
    18961 ctcaaacgtt gtattttcag ttggacgata caataaatac ccaacttcat ttgccttatg
    19021 aagttcacca ttatcaatat gaccttgatt atttttgtac gcacccccaa tgcctgtacc
    19081 aagcgtcata caaaagatcc gttctgcttg atattgatgt aatttcaatt cgcctagtaa
    19141 tgcagcgttt acatcatttt ttactttgac ataaggagac agggatttta ataatcgctt
    19201 aaaattagta cctttataat tcggaatggt tggccctgcg tatacaattt cgcctttttg
    19261 ttcatcaaca acgcctgctg atgaaatacc tatgacaggt tggatcaaat gatattgctt
    19321 catatatcct gttacaatct catatacttt gtcagtaata agctcacttt tgttgtccgg
    19381 cgttggtatt tgttgatagt caaacatatt taattgctta tcaataaccg ccgatttaat
    19441 ttgagtgcct ccaatatcga ttgcgatgta atacacctta ctcactccat ttctattgat
    19501 tttgaaaaat attttcatta attatacata aatatgacta caattttcaa aaagatattg
    19561 ttacatttta tgatagcgtt tacaattata gctgtaaact atattatcaa atgatttttg
    19621 gagggacttc ataatgaaca aagatttaaa aggtttatat gcagcgttac tcgttccttt
    19681 tgatgaaaat ggtcaagtaa atgaacaagg acttaaacaa attgctcaaa atgccattga
    19741 aactgaagaa ttagacggtc tttatgtaaa tggtagctca ggcgaaaact ttttattaaa
    19801 tacagaacag aagaagcaag ttttcaaggt tgccaaagaa gcagttgggg ataaggtaaa
    19861 attgattgct caagtaggtt cgttagattt aaatgaagcc attgaacttg gaaaatatgc
    19921 cacagaactc ggttatgatg cactttctgc cgtaacacca ttctactatc catttacttt
    19981 tgaagaaatt agagattact atttcgatat tatcgaagca acacagaaca atatgattat
    20041 ttatgcaata ccagatttaa caggtgtgaa tatttctatt gaacaattca gcgagctttt
    20101 taatcacgaa aaaattgttg gtgttaaata tacagcgccg aacttcttcc tactcgaacg
    20161 tattagaaaa gcattcccag acaaattaat catatctggc tttgatgaaa tgttagttca
    20221 agctacgatt tctggcgtag atggtgcaat tggttctaca tataacgtta atggtcgtcg
    20281 cgctagaaaa atcttcgact tagcaagaca aggtcaaatt caagaagctt accaactaca
    20341 acatgattca aacgacatca tcgaaacagt gttatcaatg gggatttatc caacattgaa
    20401 agaaatacta cgtcaccgtg gcattgatgc aggattaccg aaacgtccat tcaaaccttt
    20461 taacgaagca catcgacaaa cattagatca actcattgca aaatacgatt tatagacatc
    20521 aaagaatcaa tacattgtaa aggggatgtt gcccatgaaa gaagttggat ttggcacact
    20581 aaactgggtt gccgttatca tttatctact agctatgtta ctcattggcg tttattttac
    20641 taagcgcgcg agtcaaagta cgaatagttt ctttaccgca agtggtcgct tgccatcttg
    20701 ggtagttggc ttttcaattt atgccactac attaagtgca attacgttta tgtctacacc
    20761 agagaaagca tttttaacag attggtcata tattgcaggt aacatcgcta tcgtagcaat
    20821 tattccatta ctcatttatt tctatgttcc attctttaaa aagttaaagg taacatctgc
    20881 atatgaatat ctagaagcac gtttcggacc tagcattcgt gtcattggct ctttactatt
    20941 tgtagtttat catctagggc gtgttgcaat cgttatctac ttaccaacat tagcgattac
    21001 atctgtatca gacatgaacc cttatatcgt tgcatcactc gttggtttac tatgtatttt
    21061 atatacattt ttaggtggat tcgaaggtgt ggtttggagt gatttcattc aaggcgtcat
    21121 tttattaggc ggcgctttag ttatcattat tctaggtgtt atgaacatta aaggcggttt
    21181 tggcactgtc tttgcagatg cgattgagca taaaaaatta attagtgcag acaactggaa
    21241 actaaatact gcagcagctg ccattccaat tattttccta ggaaatattt tcaacaactt
    21301 gtatcaatac acggcgagtc aagacgtcgt gcaacgttat caagcttctg atagtttaaa
    21361 agaaacaaat aaatcgttat ggacaaatgg tatcctagct ttaatttcag cacccttatt
    21421 ttatggtatg ggtacaatgt tgtattcatt ttatgcacat gaagctgttt taccaaaagg
    21481 cttcaataca tcatctgtag tgccatattt cattttgact gagatgccac catttgtagc
    21541 aggattactt attgcagcca ttttcgcagc tgcacagtct accatttcat ctagtttaaa
    21601 ttctatatct gcttgtattt caatcgacat taagcaacgc ttcttcggaa aaggtagcga
    21661 gcgacacgaa gttaactttg ctcgtttggt catcatcatt gcaggtattt tcggttttgg
    21721 aatgtcacta tacttaattg cttctaattc aaatgactta tgggatttat tcttgtttgt
    21781 aactggatta ttcggcgttc cattggctgg tgtatttgca gttggtattt tcactaaacg
    21841 tacgaataca ttcggtgtta tttgtggatt aatattgggt atcatctttg cttacgtcta
    21901 taatggtgtt ggcaaaggta actcaccttt ctatgtatct accatttcat ttacagttgc
    21961 ttttgtcttt gcttatatac ttagcttcat tgtccctcca aaacataaaa aagatataac
    22021 gggattaaca atttttgaaa aagataaacc atcaacatac atttcaaaaa cggctacgaa
    22081 aaagtagatt gttatgataa aaccccgtca ctaagttatg atgcgctgtt gcgccaactt
    22141 ggtgatgggg ttttgatgtt gctataaatt taatttagat acttcgatta atttacaata
    22201 ctaggccaat gattgatcct gaaatgattg aagctagagt tgaaccaagt aacagtctca
    22261 ttgcaaagga tgcaactttt tctccttgtt tatcactaat gcctttaatt gaacctacga
    22321 tgataccaac cgtaccaaaa ttagcgaaac ttactaagta aactgaaatg ataccttgtg
    22381 ttcgagctga tacatccccc aggacatttt taaaatcaag cattgctaca aactcatttg
    22441 taattagttt agtcgccatt aaagagccag ctggaacagc ttcgctccat ggaatcccca
    22501 ttaagaatgc gattggtgca aacacatagc caataagctg tttaaagttc aaaccaacac
    22561 taccaaacat gatattaatt gcttccatta atgaaataaa tgctaacaac attacggcta
    22621 ctacaacagc gattttaaac ccatccattg cactatcccc aatcatttgg aaaaaggcaa
    22681 ctttcttagg ttttcctgtt tttccattca atgttttagt ttctgtggat ttcgttaagt
    22741 tatcaatttc aacatcagta tcatcagatt tatagggatt gattacactg gcgatgataa
    22801 gcgcactaaa aatatttaac attactgctg taactacgaa cttaggctca atcatctgca
    22861 tatatgaacc caacattgcc atactaacag cactcatacc agatgtcgca attgtatata
    22921 attttgctct agacaacttt ggtataatat cttttatagt taaatacact tctggctgtc
    22981 caaacatcgc tgtcgaaata gcaaagtaac tttctaaacg ccccattctc gttattttat
    23041 taatagcaat acctacatat ttgataataa atggtaatac cttaatataa ttaagaatgc
    23101 ctattaatac agaaatgaac actaatggca gcaacacatt taaaaagaat gtaaagccat
    23161 ttttattttg tatatctcca aaaacaaagt ttatacctgc tttactaaca tttattaacc
    23221 cttcaaaaaa tgaacctaat gcagttaaga ttgttaaacc aatcgttgta ttcatcataa
    23281 ataacacaat caacacttga ataaatatca ttattaacgt ttttttgaag tctatgtttt
    23341 tacgatcaaa actgaataaa aacgcaacaa atagtgcaaa tgctatccct gtgatagcga
    23401 ataaaatatt catgtatcat cacctacaac aatttattaa cagcgacagc aattttagca
    23461 cccaacgccg cattgttttc aacaagtttt atatttgctg ctaaactttt accattcgtt
    23521 ttttctacaa ttttccctaa caagaacggt gtggcgtcct tacctttaat accttgattt
    23581 tcggcttcaa caacagcttc atttatgatt gcctcaatat atgcttttga taaggcatgc
    23641 tcatatggaa ttggattagc aacaacaatg ccaccttcaa gatttaactg ctgttttgtt
    23701 aaatgaatgt cagcaagtcg ttctggcgtt tcaaccgaac ttgttaactt aacaccgctt
    23761 tcgcgagtga agaatgctgg caattcattc gtttgatatc caataactgg aacgcctttt
    23821 gtttctaaat actccatcgt cttaggtaag tctaaaattg atttggcacc tgcacagata
    23881 acagtgacat ttgttttaga cagttcttct aagtctgctg aaatgtccat cgtatgttct
    23941 gcacctttat ggacgccccc aatacctcct gtaacaaaaa attgaatacc agccattgca
    24001 gcacatatca tcgtcgtcgc tacagtagta gcaccaacac acttcatcgc aacaacttct
    24061 gctaaatccc ttctagacac tttagcaacg tctttactag ttgccagtat ttctaaatct
    24121 tcgctttcta aaccaatttt aattttgcca tctataatgg ctatggttgc tggaatggca
    24181 ccattattcc tgataatttg ctctactgtt gttgccattt caacattttg tgggtacggc
    24241 ataccatgcg aaataattgt tgattctaat gctacaatcg gttgattgtt ctcccgtgct
    24301 tgctgaactt ctcgagaata ctcaatatac ttttgtaaat ttgccatttt tataatcctc
    24361 catatcgtga taaagttgct gttgatctag gttttgccta actgtatatt tcgtttctat
    24421 cgttttcttt gcgttaacca taccagcaat taatatatct tcagtagaca tcccatttaa
    24481 ccagctatac actactgcag cacagaatga atcgcctgca cctgtaacat ctttcacact
    24541 atttgatggc ataactgact taatgatttc ttcctcacca cttcgataaa tgagttcttt
    24601 cacgccattt gtcacaataa catttttaac acctaaatca ttccagcgtt tagcagctat
    24661 ttttaaatca tcagtagatt ctatttttaa atttaagtat gtttctgttt catctttatt
    24721 cgtgataatc caatcaatag catgtaatga atcaggcata tttttcattt ttggagaaga
    24781 aaccgtggtg ataactaatt tgatttgatg tttcgtggta taggcacata agaagtttaa
    24841 tgcctctttg cctaaattca aatcgacaat aatgcactta gcctttttca ataagtgtga
    24901 acgcttaatt aaaaattcag gcgtaatgta gtcaaacact tccatatctg ctaagccata
    24961 tgtcatgtcg ccttctttac taattaaagc tgtatatgaa cctgtactcg cattttcaaa
    25021 ttgttgaaca tgatccaaat tcataaatgg tgtggacaat cgtttaatca tttcccattc
    25081 actatcttgt ccactagcag ataaaaaagc gaccgtttcg ccaagcctac ctaagttctc
    25141 agcaatattt cttgctacgc caccaataga gcgtgttgac gttacaggat ttgatgtttc
    25201 tgcaactaaa tttttatgca cataaaactt acgatctaca ttagctgcgc caatacaaac
    25261 aataggataa tcttcattta aaacatatgc ctttcccata acatattcct tttgtattaa
    25321 tcctgaaata atgtttgcta cgctgggtct agataatcca attgcctcag caagttcacg
    25381 ttgtgaaata aacggattat ctttaattct ttttaaaatt tctttctcag aatcgctcat
    25441 aacatccctc ctcatattga cttaaaatac ttgaatcagt gaatttacaa aaatatcgta
    25501 gcatgcagtt atttctgttg taaacgctta catttaccgt tatatcaatg atatttgttt
    25561 ataaaatacg cataatttcg taaaaagtaa gttcattcca tcgcgtaaaa ggcacctgag
    25621 aaaatgataa atgtttatac agtatgatat ttgtcgacta taaattatga catttttatg
    25681 tcttatattt ttctataaaa aaacgtgact cattatcgac tgttacatcg actgaatcac
    25741 gttactatgt tttatttgtt tttactattc aatatgatga tgctatgcgt ctactgcacc
    25801 tcgattaatc atgtcttgct tagtaacaat tggatatttt ttaaatacaa ttgaaccaag
    25861 ataacctgca agcgcaccta caatcgccat tactaaacca taaatcacaa ttgtcgtcgg
    25921 atgattaaat ccaaacataa ctaaaaatcc tgcaatcggt gtagctgtac ctgtcgcatc
    25981 gttaattaaa cctgacatag caataattaa accagcaatc gcaccaccaa agaaatttgt
    26041 aatatagatt ggaattggat tggctgatac aatatctgct tgtgataaag gttcaatact
    26101 tactgcaatc gtagacttac gatcacctaa ttttaagcga tggaatagcg tcccattcat
    26161 aaatgccgaa ctaaatgctg ccatggcacc aatagccata ggtacacccg ttaaacctaa
    26221 taatgctgtc aatgccattg aactcaatgg cgctgtgccg acaaccgtaa taataccacc
    26281 taaaatgata cccatgataa ttgggttcgt attcgtacta ctttggataa tatcaccaat
    26341 tcgaatcaat gtactattca ctactggcgt taataatact gcaataagac gagctaatgg
    26401 cgctaagatg acaatcgatc caattaagtc gacaccatcc ggcacatatt tctctgtata
    26461 tttcatcaca tatccaatca tataacccgc aaagaaacct ggtaataaat ccataccacc
    26521 gcacgcggct gcaattacta atgcatatac tggtgacaca ccgatagcta atgctgttaa
    26581 tccagcggca gcgacaccac ctagacctcc cgcagcgtct cctagctctt ctaaaaattt
    26641 aataccgaat acttcgccac caacatattt attaaatgct tctactaaaa atgttgcgat
    26701 tgctgcatta gctaatgctc ccatggcacg cataccatta ggtgctttat atgtaaataa
    26761 tgtaaaaatc actaagacca aaaataaaaa taaagtacct atcaataaat ccataaccat
    26821 tgacccccat tacgataatt ttatattcaa tccccatatt taaatattga atacttagag
    26881 gccattttac tacaaaaaat attatatttc acggaatatt tctaattttc gataataatt
    26941 attattaatt gaatgtgaaa gtttattcaa ataatacata aacataaaaa taccggcaca
    27001 tgatgtaccg gcttaataaa actattctac tgtttcattt tcaagattaa ggacgccatc
    27061 tttcatatga tatgaacgat cacaatatgc tttaagtcgt tcatcatgtg taacaataat
    27121 acatgctttc tttctttgtt tggcttgatc acgtagaatt ttaatgactt caatcgcatt
    27181 ttcagtatct aacgccgcgg taggttcatc cgctaaaata atcgacggat tcgtatataa
    27241 cgctttggct atcgccacac gttgtttctg accacctgaa atttctgaag gtaacttatt
    27301 aagcaatgaa gttagaccta attgtgacat aagttgctga tagtcttcat tagacataac
    27361 attcttattt ttctttttca ataatgtaaa ttgttgcttt accgttaaaa atggcacaag
    27421 atttgtagct tgtaaaataa aacctatctc agacattcta acttttgcca atgctttttg
    27481 cttcattgtc gtaatatctt ggttattgat taaaatgtgc ccagatgtcg gcgtttgtaa
    27541 tgcacctgcc atagttagaa atgtactttt accagagcca gaaggtccaa ccaatgctat
    27601 aatatcacct ttatttatct caaaatttgt atctttaacc gcttcaatgt tacgattccc
    27661 atctttaaat gactttgtta cattttcaaa tttcaacatt tgctacacct cctacccaat
    27721 cgcctttaac ggatctattt ttctaattgt taaaatggag aataaacttc ctaatacaga
    27781 gacaatcatt aacacaattg caaatacgag caatgttagt acatcgaatt tgacaggtac
    27841 tgcatcaggt aaaaatgcac ctgtaacgcc tgttaacagt aagccaaatg ccgtaccaaa
    27901 taatgctaat atgagcgtct gcgaaataac tacgttcgct aaatagccat tcgtaaatcc
    27961 ttgagccttt aatatgccaa ataaacttgt tttttgtaat gtcatgacat ataagaaaat
    28021 gcctataact gtagctgaaa tgacaaataa gaatgaaatc ataaagttta atgttaagtt
    28081 ctgcggttta taacctggta aattttcaat aaagtcatta atacttaccg cttcaagctc
    28141 ttggtttaat tttttgtctt tccaatttgt atcacgtaca acaactgcat taattttatc
    28201 gccagtcaat ctaggattga ttttggcaat ggtagcgtca ttcgtgaaaa tgactggtga
    28261 cgcattatat tttgcacttt ctgtaaaacc tacgatatgc aatttttcat ctgattgaga
    28321 tagtgataat gtgtcgccaa ttttaaatcc tttattttta agcgtttcat cagctaacac
    28381 ttcattatct ttagtcgctt tatgcccttc tattaaactc ggaactaaaa atgatgactt
    28441 ttcaacaccg aacactaaaa cattgtcttt ttgatggcca ttagacacaa tttcccctgt
    28501 ttgcttcaaa gtagcttgct tcttgtattt attttcaata tctttcttgt taaaaacaga
    28561 ttgttgcaca gtttgattgg catctttatt tagaacaatg gcatctgctt gccacttatc
    28621 aatgccttct ttattcatat tgataagacc attcgccaat ccagataata aaaatagcaa
    28681 gtaactaatc atcgttaaca caccaataat tagtccaaac ttcaatttgt tgcgccgtat
    28741 ttcattccaa gctaaaaaca tgcatttctc tccctactac tatgatttaa acattgttta
    28801 tattcttaga tgcacgtacg tcgtgttgcg ctctgtaatg ttatacatac acttatcctt
    28861 cattataccc gaacttttta tattaaaagc aaatttatgg aaaatgcaat taattgtcta
    28921 ttatttttgt acggtacatt taaaattaag gatcaattta aaaacgccta catatacctt
    28981 taagtacatg tagacgtcca attcatatat tatttaactt cgcctgtttt aggatcgaat
    29041 tgcttaatag catttttacg taatttatct tttgctttgt cacttgcttt atagttattg
    29101 ttgtaaattg tagcttccca actaccatac attgggttag ggaaaatgat atatttctta
    29161 ccgaaatcgt ctttatgttt ttcaattaat gcttcacgag attcagctgt agcttctttt
    29221 ggatctgtaa agtctaataa attatctcca aatagcataa caagtttatg atccttttga
    29281 actatttgtc tgcgtgattc tttactctta tcatctttac cttttagtaa aatatgactc
    29341 ttcttagctt gagggatacc ttgttgtttt aagttctttt gtgttgcctt taaatctttt
    29401 tctttatctc tatcagaaat atagtagata tcgacacctt ttttgtcagc atatttcaag
    29461 aattcttttg cgccatagac aggtttagct ttagcagctt gtacccattc atgccaacct
    29521 tctgggaaag gtttattatg tattgatgca tagccttgat atggagaatt atctaaaact
    29581 gtttcatcta aatccaaagc aatagctaac ttatgtttac ccttattctt tttaatctct
    29641 ttatctaact gagtctttgc actgttataa ccttgtaaat ataatgcttt tgcttcagct
    29701 gaattttgat accaagccac tgccataata ttttgattac caagattcgc tttttgtgaa
    29761 gccggcattg aagcttgttg cgtttgttga acttcagcag aacttttggc aaacgctgta
    29821 gaatttgtcg tttgtggtgc cgaaactgta accgctaccg ataatgatgc tattgcaata
    29881 tactttgaaa ttttattcat cttatcacct catgattaat atttaaaata cagttaaaat
    29941 tataaatgca tttatttaat attgctatac tatgaaaaga tatttaatat tatttcttga
    30001 aaagctaaca aatatgtgaa catttcatat aagcatgatt taattggcga cctatcacat
    30061 gagagagatt tgtgatttat cacgtaataa actttaattc atttgagtga ctaagccttc
    30121 ctatcagaca tattcactca tccacgtatc attatgtgta cagtgtgcta tctcttattt
    30181 acctattgga acaaccataa actcatccat agtttacctt ttataaatag cagtcctcac
    30241 tcttacaatt tctcataaaa atcacaacgc tccaacgtat ttccaactta cttccaccta
    30301 ttttaattca taaaaacgac actttaattg tcattatcca ataatagcaa gacgttatta
    30361 ttgcaatctt ttttataaaa taatagaatc atagtattgt catttaaaga taaagtaaga
    30421 acgtttttat ttttcagatt ttttaaatta ttatgaatat ctagttttag gaaggaaatt
    30481 acattgaaaa aacaagttat tatttcgggc ctcatgttat tttcactatt ttttggagcc
    30541 ggaaatttaa tattcccgcc catgcttggc catacagcag gtcaaaatat gtggattggt
    30601 atgttaggct ttgccattac aggcatatta ctccccttta ttactgttat tgttgtcgca
    30661 ttttatgatg aaggtgttga aagtgtaggc aatcgtatac atccatggtt cgggtttatt
    30721 tttgctgtcg tgatttacat gtctatcgga gcattttacg gtattccacg tgctgcaaat
    30781 gtcgcgtacg aaattggtac aagacacatt ttacctgtgc acaaccaatg gactttaatt
    30841 atattcgcag caatcttttt tgctatcgtt tactggatta gtttaaatcc atcgaaaatc
    30901 gttgatcatt taggaaaatt attaacaccg ttattactat taatggtcgc tctattaagt
    30961 attgctgtca ttttcaaccc tgaatctgca ctaagtgcac ctaaggataa atatataaca
    31021 catcctttca ttgcaggaag tttggaaggc tattttacaa tggatctcgt tgcagcatta
    31081 gctttttccg tagtcattgt caatggctat aagtttaaag gcctcacaga tcgcatgaaa
    31141 atattaaagt atgtctgctt ttcaggtctt attgcagcca tattacttgg tatgatttac
    31201 tttgcgttgg catatgtagg tgcgtcaaca gctccaggaa actttaaaga tggtacagat
    31261 atattgacgt acaactcatt acgagtattt ggttcatttg gcaacatagt atttggtatg
    31321 acagtgatac ttgcatgttt aacaacatgt ataggactcg tcaatgcttg cgccacattt
    31381 actaagaaac acgtacctaa gttttcttat aaaatattcg cacttatttt ctctatcata
    31441 ggattcttat ttacaacact tggtttagaa atgattttaa aaattgctgt accattgttg
    31501 acattaatat atcccgtgtc tattgcactt gtactgatat catttgctaa catgtttagc
    31561 acattcagat tcagttgggc ctatcgactc gcaactgtta ttacattgat tatttcaatt
    31621 ttacaaatac taaatagttt caacttatta cacggtgtta ttttgaaatg gtttatgatg
    31681 ttacctttag cagatatcga tttagcttgg cttgtaccat tcattctttt ggcaatcata
    31741 ggtttcatca tcgatgtagt tatacgccgt ccgaaacaag cgacaactaa atagacgcat
    31801 aatctcctag taatgatcgt cgcatcatta ctaggttttt ttgcatgaaa atctgtattt
    31861 attcattgat actaactgac gattaattat tttaaaataa atgatttgtt aaaaaatcct
    31921 agctgttatt caaaaatatt agtttttaaa attaattttg tgaaattatt cacaaataaa
    31981 gtgattcata gtatactctg attaacaaat gaaaagtgag gatgtcattg tgaaggagaa
    32041 acatattaaa tgggataaaa tattttatgg ggacgactgg gtaaataacg tagtcgaaac
    32101 gataagaacg aaagatatat tacaaagcgt ttatttaaaa cgttatttat tacgcgctat
    32161 gatggcagga tttattatcg gtattattac cgtcttcgtg ctatcagtta aagcaacaca
    32221 cgaaccagat ttaccaccag gtatagtgaa tatggccagc gccattacat tcagctttgc
    32281 gttagtactc attttattta caaactccga actactaacc agtaacttca tgtactttac
    32341 tgtaggcctg tattataaag taattaaacc gactagagta ttgaaaatat ttttattgtg
    32401 ctttgcagga aatattttag gtgctgccat tttatttagt ttcatgcgtt tttcaaatgt
    32461 aatgacgcca gatatgttaa atcagttatc agcagttata gagcataaaa cgttgtctac
    32521 tggttttgta agtatattaa tgaaagcgat attcgccaat ttctttatca atatttcatt
    32581 agttattgcc atgcaaattg acgatgtact cgcaaaaatg tttgtcatga tgttcggtgt
    32641 taccatattc gcctttatgg gttatgaaca cgtcgtttac aacagttgct tatttatggg
    32701 tggtttaatt tatcaagttg atacattgca cttcatacca gctatttcga atatcgctgc
    32761 ggcttttatc ggtaactata ttggaggcgg actcattata ggtttattct atgcttactt
    32821 gaatgatcat catcaatttt ataaaaataa ttaactatat ttgaacaatg cgttagtcga
    32881 tgcctttaat atttagaagt gcattgtcta acgtattttt ttaaagttcg tcaatggttt
    32941 ttatccgatg ctatgagcat tatcaaatac aaaatgctct cttaaaagca gttattgact
    33001 gaaaatctac ttctaagaga gcactttatt taatcggtta agaaatcttg aaatttcaat
    33061 atacgatgtt tatgataagt cgcttatttc atctttaggc ttgttattag taagtagttt
    33121 aataccactg actaaccata aagcaaatgt aattatgtta ccacttatta cagctccaat
    33181 aatcaacaat ataccactca tttttttgtt tttagatgct ttaaacatac cgattgcacc
    33241 taaaataatt gaaatgattc caaatataaa taggaataag aataatacag tgaaaattgc
    33301 tgctgctgtt tctgcatcaa ctgggtcaac ctcaccatta actgttgttg acacatctgt
    33361 agtcattaca cctgatgctg ctaaaaccat aaatagtaaa ctaaataccg ttgctaaacc
    33421 ctgaacaaca attcctatta ttgtcattat tttgatcgat ttttccattg tcttactcct
    33481 ttattattat ttacaaactt acgtttgtgt tcttatatat aaatgtttaa atttatatcg
    33541 tgtttatttt aaaaattatt gtcgctaaag ctcatttact tttcgaaact ggttaa
//
LOCUS       DAPIDM010000014        33171 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000014 DAPIDM010000000
VERSION     DAPIDM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.014"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(11..625)
                     /locus_tag="RJE42_000591"
     CDS             complement(11..625)
                     /locus_tag="RJE42_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5876043.1"
                     /translation="MTNKKIDPRVKRTNQYLINAIVKLLNDKDINKISIQNITDSADL
                     TRATFYLHYRDKQDFFNRIVTNVIDDLIDYVKDGHLIASGLNNESDIKNAFTRLFEYI
                     YMNYEFFSVMLSDRGLSQFRPKLESVVQKEIYIPLFHALVKIDGEKTSKFPQHYFLNY
                     ITSAHIGVICTWLNDDMNYSPKYMAELLYKLTLEGIFSVVRSEF"
     gene            808..1785
                     /locus_tag="RJE42_000592"
     CDS             808..1785
                     /locus_tag="RJE42_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV5876044.1"
                     /translation="MANKTGYIDIHHHIIPEFYLDALKEAGIDKAGGLKINNWQPENS
                     IKMMDRLGIEVGITSISDPVVEPLEPSKRKAIAREINLLQKQIIKEYPNRFGAFATLP
                     LPDVDSAIEEVKYALDNLQLDGVGLLSNYNEVFLGDPQFEPLMKVLDERATVVFIHPS
                     TPPDYVPRPKYMPVDFMAEFTFNTTRAAANLILSGTMDRYPNIKFILAHAGGTLPYLT
                     WRINECYKLIKQTADESQFVVLAKDPEAYVSDFYYDTALSTQSATFRALEETTLEDHI
                     LFGSDAHYAPENLDQSMINTIKHQLDLKNETIENIKYNSALKLFPRFQK"
     gene            2134..3318
                     /gene="rocD"
                     /locus_tag="RJE42_000593"
     CDS             2134..3318
                     /gene="rocD"
                     /locus_tag="RJE42_000593"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDV5876045.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGGKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLDNEQLIERSERLGSILLKELLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVEQLIKRGILCKDTHRTIIRLSPPLVIDKEEINQIVAAFQDVF
                     KN"
     gene            3357..4388
                     /locus_tag="RJE42_000594"
     CDS             3357..4388
                     /locus_tag="RJE42_000594"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV5876046.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAASQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIIDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGSHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLKQSPVYP"
     gene            4400..5641
                     /gene="argJ"
                     /locus_tag="RJE42_000595"
     CDS             4400..5641
                     /gene="argJ"
                     /locus_tag="RJE42_000595"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDV5876047.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DAQQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHINDANFTTPGAFNEA
                     ILTTDTCTKHIAVSLNIDGKTITIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANQQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5657..6427
                     /gene="argB"
                     /locus_tag="RJE42_000596"
     CDS             5657..6427
                     /gene="argB"
                     /locus_tag="RJE42_000596"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV5876048.1"
                     /translation="MKFIVIKIGGSTLSDMHTSIINNIKHLRSNNIYPIIVHGGGPFI
                     NDALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQQQCSAIGLCGLD
                     AQLFEIKRFDQQYGYVGVPTTLNIDSLSYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIAASLEAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIEAIYNNDFVGTTILKSSV"
     gene            6711..7190
                     /locus_tag="RJE42_000597"
     CDS             6711..7190
                     /locus_tag="RJE42_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV5876049.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAIGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(7519..8163)
                     /locus_tag="RJE42_000598"
     CDS             complement(7519..8163)
                     /locus_tag="RJE42_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV5876050.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(8176..15351)
                     /locus_tag="RJE42_000599"
     CDS             complement(8176..15351)
                     /locus_tag="RJE42_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDV5876051.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEVTKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHLNRNDDKDNQDALHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDLASLAVSVYLANYIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTFDAKDVCQ
                     RFTTDFNKSVLQNMSQLQCAKSSLSLETIFHCYHQMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVQNIYLQITEENGNKRTTVDELNLMTE
                     SDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AQRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQVVFNNNDDCIPHFELDTIVWEEVDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEKTTILLSGTIAFDAATFEIYGALLNGGKLIVAKT
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQRPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNIPFLWRLSSE
                     LNVAQLRQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFKEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSLDKMWTQFLQEVKEMSLEAYEHQEYPFEFLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDCDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGKGQRVALIT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPKKRQGAILEDAKVTAVMSYGIEIETTLPVI
                     QLENAKCFVESKENEQYDDLHGDQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVYAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPAQLQ
                     HLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKQCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITHIDCIPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLIQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGISLAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTKLTLKIMD
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15798..17048)
                     /locus_tag="RJE42_000600"
     CDS             complement(15798..17048)
                     /locus_tag="RJE42_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876052.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIINIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTTLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(17433..18458)
                     /locus_tag="RJE42_000601"
     CDS             complement(17433..18458)
                     /locus_tag="RJE42_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDV5876053.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18631..18990)
                     /locus_tag="RJE42_000602"
     CDS             complement(18631..18990)
                     /locus_tag="RJE42_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDV5876054.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(19207..20238)
                     /locus_tag="RJE42_000603"
     CDS             complement(19207..20238)
                     /locus_tag="RJE42_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDV5876055.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEATLR
                     KNAEVIEKVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEGFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(20251..21012)
                     /locus_tag="RJE42_000604"
     CDS             complement(20251..21012)
                     /locus_tag="RJE42_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876056.1"
                     /translation="MTRLTINKIILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQYLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(21009..21983)
                     /locus_tag="RJE42_000605"
     CDS             complement(21009..21983)
                     /locus_tag="RJE42_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5876057.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQHQQVIKIGYLPI
                     THSANLMMTKKLLSQHNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEALREQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKAVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(21997..22737)
                     /locus_tag="RJE42_000606"
     CDS             complement(21997..22737)
                     /locus_tag="RJE42_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876058.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIQPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTKSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNDIMETFALNHHQVEPEYYL"
     gene            complement(23079..23591)
                     /locus_tag="RJE42_000607"
     CDS             complement(23079..23591)
                     /locus_tag="RJE42_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDV5876059.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKASSLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEAGISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            23771..23974
                     /locus_tag="RJE42_000608"
     CDS             23771..23974
                     /locus_tag="RJE42_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876060.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVNTIKDIDENHLDDAT"
     gene            complement(24036..24995)
                     /locus_tag="RJE42_000609"
     CDS             complement(24036..24995)
                     /locus_tag="RJE42_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDV5876061.1"
                     /translation="MNGKKANTINRYKYFHHVDHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(25641..27128)
                     /locus_tag="RJE42_000610"
     CDS             complement(25641..27128)
                     /locus_tag="RJE42_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDV5876062.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVEVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            27555..27938
                     /locus_tag="RJE42_000611"
     CDS             27555..27938
                     /locus_tag="RJE42_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDV5876063.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNNKIEE"
     gene            27945..28271
                     /gene="isdI"
                     /locus_tag="RJE42_000612"
     CDS             27945..28271
                     /gene="isdI"
                     /locus_tag="RJE42_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDV5876064.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(28335..29480)
                     /gene="cap8P"
                     /locus_tag="RJE42_000613"
     CDS             complement(28335..29480)
                     /gene="cap8P"
                     /locus_tag="RJE42_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDV5876065.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIHADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAENLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DKAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRRK"
     gene            complement(29557..30819)
                     /gene="cap8O"
                     /locus_tag="RJE42_000614"
     CDS             complement(29557..30819)
                     /gene="cap8O"
                     /locus_tag="RJE42_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDV5876066.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIEADARTAEMSKLMENTYRDMNISLA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(30873..31760)
                     /gene="capN"
                     /locus_tag="RJE42_000615"
     CDS             complement(30873..31760)
                     /gene="capN"
                     /locus_tag="RJE42_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDV5876067.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KGYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTANYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSTLYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(31760..32317)
                     /gene="cap8M"
                     /locus_tag="RJE42_000616"
     CDS             complement(31760..32317)
                     /gene="cap8M"
                     /locus_tag="RJE42_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDV5876068.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(32328..>33171)
                     /gene="cap8L"
                     /locus_tag="RJE42_000617"
     CDS             complement(32328..>33171)
                     /gene="cap8L"
                     /locus_tag="RJE42_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDV5876069.1"
                     /translation="HRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYINRHVY
                     KNAENVIVLGTEMKNYLVNHQISKNPDNIHVIPNWYDMRQLQDNHIYNDTFKTYREQY
                     DKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDHRIENV
                     KMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMDKQSDI
                     VQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREINTMKYY
                     NLLK"
BASE COUNT        10745 a         6250 c         4993 g        11183 t
ORIGIN      
        1 caactattat ttaaaattca cttcgtacta ctgaaaaaat accttctaaa gttagtttat
       61 atagaagttc agccatatat ttcggactat aattcatatc atcatttaac caagtacaaa
      121 taacaccaat atgagcggac gttatataat ttaaaaaata atgttgtgga aatttacttg
      181 ttttttcacc gtctatttta acaagtgcat gaaacaatgg tatataaatt tccttttgta
      241 ctacagattc taatttcggt ctgaattgtg atagacctct atcacttaac atcacactga
      301 aaaactcgta attcatataa atatattcaa ataatcgcgt aaatgcattt ttaatatctg
      361 attcattatt taagccagat gctatcaaat gtccatcttt tacataatct atcaaatcat
      421 ctataacatt tgtaacaatt cgattgaaaa aatcctgctt atctcgataa tgcagataaa
      481 acgttgctcg agtcaaatct gctgaatctg taatattttg tatagatatt ttattgatat
      541 ctttatcatt gagtagtttt acaattgcat taattaaata ttgatttgtt cgctttaccc
      601 gtgggtcaat ttttttattt gtcattttca gcacctctat ctatcatttt ccactttatt
      661 tacacattta atcgatttgt attttaattt acaaattaac atattttgtg aattgcaatt
      721 aattgtgttg cgagtacaat gaattatata cattttgtct ataaatagtc aatttgttca
      781 ttttgaagtt ttaggaggat ttatattatg gcaaataaaa caggttatat tgatattcat
      841 catcacatca ttcctgaatt ttacctagac gcattaaaag aagctgggat tgacaaagcc
      901 ggtggtctaa aaattaacaa ttggcaacct gaaaacagca taaaaatgat ggatagattg
      961 ggtattgaag tagggattac ttccatttct gatccagtcg ttgaaccact tgaaccaagc
     1021 aaaagaaaag caattgcacg agaaattaat ttattacaaa aacaaatcat caaagagtat
     1081 ccaaatcgtt ttggtgcttt cgcaacattg ccactacctg atgtggatag tgcaattgaa
     1141 gaagttaaat atgctttgga taatttacaa ttggatggtg taggtttatt atccaactac
     1201 aatgaagtat ttttaggcga tccacaattt gaacctttaa tgaaagtact agatgaaaga
     1261 gcaacagtcg tgtttataca tccaagtaca ccacctgatt atgtacctag accaaaatat
     1321 atgcctgttg atttcatggc tgagtttact tttaatacaa cacgtgctgc agcaaactta
     1381 attttaagtg gtacaatgga tcgctatcca aacattaaat ttattttggc acatgccggt
     1441 ggtacattac catatttaac atggagaatt aacgaatgtt ataaattaat caaacaaact
     1501 gcagacgaaa gtcaatttgt tgttctagca aaagatcctg aagcttatgt tagtgatttc
     1561 tattatgaca ctgctttatc aacacaatct gcaacattta gagccttaga agaaactact
     1621 cttgaagatc atattttatt cggtagcgat gctcactatg cacctgaaaa tttagatcaa
     1681 tcaatgatta atacaataaa gcatcaactt gatttaaaga atgaaacaat tgaaaatatt
     1741 aaatataata gtgcacttaa attattccca agattccaaa aatagtttaa acctaaatat
     1801 gttactgctc atataaatat aagtacagtg ctattgagac taatcattca tctagcactg
     1861 tactttttta tgtaacagtt tcaaaaagca attcgattat tactatcgtt tatcaccttt
     1921 atcacaccac aaaacttgta aatcgctatc ttgattacat ttaaatattt tccaaaatca
     1981 caaaaaataa atcgcaataa ctctactcta ttttaaaaac acagaaatat tatgcataaa
     2041 ttattaaatt tacatttgac acttatattg aacaactgta atatattaat attaattctt
     2101 taaaatgtat aaatataaag gagggggaga cccatgaatt ctatcattga attaactgat
     2161 tattatagct ctaataatta tgcaccactg aagcttgtca tttctaaagg taaagggggc
     2221 aaagtttggg atactgatgg caaacaatat atagattgca tttcgggttt ttcagttgca
     2281 aaccaaggcc attgtcatcc aacaattgtt aaagcgatga cagaacaagc ttcaaagttg
     2341 tctatcattt cacgtgtcct ttatagtgac aatctcggga aatgggaaga aaaaatttgt
     2401 catcttgcta agaaagacaa agtactcccc cttaactctg gtactgaagc tgttgaagca
     2461 gccattaaaa ttgctagaaa atggggctct gaagttaaag gcatcactga cggacaagtt
     2521 gaaatcatcg ctatgaataa caattttcac ggtcgtacac ttggctcatt atcactatct
     2581 aaccacgacg catataaagc aggatttcac cccctacttc aaggcactac aacagtagat
     2641 tttggagaca ttgaacaatt aacacaagct atttcaccga atacagcagc aattattttg
     2701 gaaccaattc aaggtgaagg tggcgttaat ataccaccga aaggatatat tcaagctgtg
     2761 cgtcaactat gtgataaaca tcaaatatta ttgattgcag atgaaattca agttggtctt
     2821 ggtagaactg ggaaatggtt tgctatggaa tgggagcaag tcgttccaga catttatatt
     2881 ttaggtaagg cattgggtgg cggcttatac cctgtatctg ctgtacttgc aaataatgat
     2941 gtcatgcgtg ttctaacacc aggtacacat ggttcgacat ttggtggtaa ccctttagcc
     3001 attgcaatat cgacggcagc gcttgatgta cttgacaatg aacaactgat tgaacgatca
     3061 gaacgcttag gttcaatttt attaaaagaa ttactacaac ttaaacatcc aagcattaag
     3121 gaaattagag gtcgtggttt atttattggc atcgaactta acacagatgc cgcacctttc
     3181 gttgaacaac tgattaaacg tggcatttta tgcaaagaca cgcatcgtac tatcattcga
     3241 ttgtcaccac ctctagtcat tgataaagag gaaatcaatc aaattgttgc tgcttttcaa
     3301 gacgttttta aaaattaaca attaatcatt tatatatgat ataggaggga ttcatgatga
     3361 ttaaagtagg tatcgttggc ggtagcggtt atggcgcaat tgaattaatt cgattattac
     3421 aaacacatcc tcatgtaacg attgcacaca tctactcaca ttcaaaagta gatgagccgt
     3481 tgaaattaac atttccacat ttacaacata ttatgcaaca tttcgaagca cttacagtgg
     3541 acaataatga ctgtgatgta attttctttg ctacacccgc acccgtgagt aaaacatgta
     3601 tccctccttt agtagaaaaa ggaattcatg ttattgattt atctggtgca ttcagaatta
     3661 aaaaccgtga aatatatgaa gcatattaca aagaaactgc tgcatcacaa gacgacttaa
     3721 atcacgctat ctatagcatt tcagaatggc aatcgtttga taacaatgga acgaagctca
     3781 tttctaatcc tggctgtttc cctacagcaa cattattagc attacatcct cttattagcg
     3841 aaaaaattat agatttgtca tctattatta ttgatgctaa gaccggtgta tcaggtgctg
     3901 gacgttcatt atcacaacgt gttcattttt cagaaatgaa tgaaaatcta agcgcttatg
     3961 caatcggaag ccataaacac aaacccgaaa tcgagcaata tttatctatc attgcgggtc
     4021 aagatgtatc agtcatattt acaccacatc tcgtaccaat gacacgaggt attttatcaa
     4081 caatatatgt caaattatca tctgaataca cgactgaatc attacataaa ttaatgacct
     4141 cttattatgc taatcagcca tttgtcagaa ttagagatat tgggactttt ccaaccacaa
     4201 aagaagtact cggtagtaac tactgcgata tcggtatcta tgtagatgaa acaacgcaaa
     4261 cagcaatttt agtatcagtg attgataacc ttgtcaaagg ggcaagtggt caagccattc
     4321 aaaacttaaa tatattatat gattttgaag ttacgactgg cctaaaacaa tcaccagttt
     4381 atccataagg ggtgttagaa tgaaacatca agaaacgaca tcacaacaat ataacttttc
     4441 aattattaaa catggcgata tcagtacacc tcaaggcttc acggctggtg gtatgcacat
     4501 cggtttacgc gctaacaaaa aagactttgg gtggatttac tcatcgtctt tagcaagttc
     4561 agctgccgta tatactttaa accaatttaa agctgcacca cttattgtca ctgaagacac
     4621 tttacaaaag tctaaaggga aattacaagc acttgttgtt aattcagcta atgcaaattc
     4681 ttgcacaggt cagcaaggta tcgatgatgc acaacaaact caaacatggg ttgctcaaca
     4741 acttcaaata ccatctgagc atgttgctgt tgcttcaact ggggtcattg gagaatattt
     4801 accaatggat aaaattaaaa ctggtacaga acatattaat gatgctaatt ttacaacgcc
     4861 aggtgcgttt aacgaggcaa ttttaacaac tgatacatgt acaaagcata tcgctgtatc
     4921 actaaatatt gatggtaaaa ctattacaat tggtggcagt gctaaaggat caggtatgat
     4981 tcacccaaat atggctacca tgcttgcttt tataacaacc gatgcatcaa ttgaatcgaa
     5041 tacacttcat caattattaa aatcttcgac tgaccataca tttaatatga ttactgttga
     5101 tggcgataca agtacaaatg acatggtatt agtcatggca aatcaacaag ttgaacacca
     5161 aatacttagt caagaccatc cacaatggga aacatttgtt gatgcattca attttgtctg
     5221 tacattttta gctaaagcta tagccagaga tggcgaaggc gcaacaaagt taatcagcgt
     5281 caatgtgtca ggtgctaagt caatatcaga tgcaagaaaa atcggcaaaa ctatcgtaag
     5341 ttcaaatcta gtaaaatcag ctatttttgg cgaagatgcc aattttggtc gaatcattac
     5401 agctattggc tatagcggat gtgaaattga tcctaactgc acatatgttc aactgaacca
     5461 aatacctgtc gtggataaag gtatggctgt actatttgat gagcaagcta tgtcgaatac
     5521 attaactcat gaaaatgtca caattgacgt tcagcttggt ttaggtaacg ctgcagcgac
     5581 tgcatacggt tgtgatttat cctatgatta tgtgcgtatt aacgcatcat atcgaacata
     5641 aggtggtgtt ggttagatga aatttattgt cattaaaatt ggtggcagta cactaagtga
     5701 catgcataca tcaattatta acaacattaa gcatttacga tcaaacaaca tctaccctat
     5761 tatcgttcat ggcggtggcc catttattaa tgatgcatta tcaaaccagc aaatcgagcc
     5821 acactttgtt aatggcctaa gagtgactga taaagcaacc atgactatta ctaaacacac
     5881 gctcattgca gacgttaaca ctgcattagt agctcaattt aaccagcagc aatgttctgc
     5941 aataggctta tgtggtttgg atgctcaatt atttgaaatt aaacgttttg atcaacaata
     6001 tggatatgtc ggggttccga ccactttaaa tatagactca ttgtcttatt tatgtactaa
     6061 atttgtacct atcatcaatt cgattggttt caataaccat gatggagaat tttacaacat
     6121 aaatgcagac acactcgcct attttattgc agcatcattg gaagcaccta tttatgtact
     6181 aagtaatatt gctggcgtgc taatcaatga tgtggttata cctcaattgc cattagtcga
     6241 tattcatcag tatattgagc atggtgatat ttatggaggt atgattccca aagtgctaga
     6301 tgccaaaaat gcgattgaaa atggctgtcc taaagttatc attgcatcag gaaacaagcc
     6361 aaatatcatt gaagctattt ataataatga ttttgttggg acaacgatac taaaatcatc
     6421 agtatgactt tgaatatgac cactcaatct tgtattattc ggcttcgaaa catagttgta
     6481 cctatttgtt taatgattat gcagcgtcac cttttcacct gtctcatttc acaaaagaaa
     6541 tttcagaata tagtatgata caatatgaga tgaaaaacac tataagtgat tttcaaattt
     6601 gtaactttaa atattttata ttttaaaagt ttttcattgt gattattttc acaaaatact
     6661 ataatgagga tagtaaatag agaggagtcc ttaagttgac aaaacgacaa atgggtatat
     6721 tcatttatgc tggaattatc ggtggcttgt tatctggaat tgtaaaatta ggttgggagg
     6781 tcatgtttcc acctcgcaca ccagaacgta atgcaacgaa cccacctcaa gagttattgc
     6841 aacaattagg atttagtagt gagtttacgc atcaaacata tacattttca aatatggaat
     6901 tgccttgggt gagctttatt gtccacttta gtttttctat cgtcattgca attatttact
     6961 gcatattagt taaaaaatac gcttacttag caataggaca aggtgctgta tttggtattg
     7021 ctatttgggt attattccac cttatcatta tgccaatcat gcatactgta cctgctgtgt
     7081 gggatcaacc attccaagag catctgtcag aattctttgg ccatatcgtc tggatgatga
     7141 caattgaatt agtgcgacaa cattttgtct atcgctataa attaaattaa tacactgact
     7201 aacattaacg tgagttttaa atcatcgttt gagtatgatg attgatgctc acgttatttt
     7261 attaactgac atgatataat tctagccaac ttacatgagc attaaagtct caaatgcgtc
     7321 gtaacaagcc attatttttg gcaatttcaa tattgctcag tatattttca ccttatcact
     7381 tactttaatc tcgtcatgat tttgaatgat gccatcgtgt attcaccttt catttttcca
     7441 ataaaaaaac atctaacagt aaacatttag gcagtatagt ttaaaatcac tgcgcaatga
     7501 tactgttaga cgccatgttt agctactcaa taactgaaat acagacactt ttttataacc
     7561 ccagggtgcc tgtcctaaga aacatacctg taccataaac tgatcaaaaa taaattgttt
     7621 gaacttcact tcacgtgatt gataaaagtg tgattgcgtc atatcataaa tgtccaatcc
     7681 tttgattaaa ccttcaccaa tcaattttgt aaaactttct ttttgtgtcc atatttgata
     7741 aaaatcattt aaactacata tttgatgtgc ttcgtttgta gagaaacacg tcactaatgt
     7801 acgccagtct aaacgttgtg atatcttttc gatatcaata ccaactggtt ctttatcgac
     7861 aacacacacg atataaggat aactatatga taagctcaca tagatgggct gtccatcacg
     7921 attgtgttga actatatctg ccttacctcg tggcgaaatg tgataatgcc attcatgtgg
     7981 taataaccct gtgtcatgtt gaattccata ttgcactaaa atatctccca atctgtgcat
     8041 gagtttatct tgattgtatc tatagttgac tgtacgcggt tttttatatg accaacgact
     8101 ttgtgatatt aattcttcaa tacttttcaa gttactctgt aattgcatta caaatactgt
     8161 cataactttc cctacttact tattgaatat tgttttgata tattgtgccc aatgatacag
     8221 ccaattgtta gttatcgttg gccatttttc actgatgtga tccattattt ttaatgttaa
     8281 ttttgtatct atcattgcta gttgttgttc acggtcaaca ctagttaatc caatcgtttc
     8341 gtacatgtct tgtttctgta agatttcatt aaatgattca tcgctgacga gttcaatttc
     8401 tttgcgctta acgcattcta acaaagattt caccggcatt ttattaggtg ataacacatg
     8461 gtaaatgatt tgtggcgtgt tgacttgtgc taatgcgaca atttgtcttg cagtcgtatc
     8521 cacaaaagaa aaatctacag gcatttcagc caggctaatc ccgatacaat ccagttgtaa
     8581 caaatcattc attaccattg aaaaacggtt agtctttata tttctcatat gccatcttcc
     8641 attgtaagga ctcgtcaaat taccaacacg tacaatccga ccatctaagc cattatttac
     8701 agcttctaat acttttaatt cactataaaa tttactccgt gtatatggcg atgttagtag
     8761 ttgtccttta tagacatccg cttctgaaaa cgtcacatct tctgtgtcta tatcaaaata
     8821 agttcccaca cttatcgtag acacatatat taaccttgca tgatgttgtt gtgccaaacg
     8881 tatgacatca acagtacctt gaacatttac tttttcaaat tcatcatcat caccaaagtg
     8941 atctgtacga gcacctgcat gaataatcgt atccatgttt tctggtaaaa caacatcatc
     9001 catacactcg aaatcaccaa caatgacttc aatgtttgat aacatcattt caaccgtctc
     9061 ttctgaaaaa taatcattta aattcgtcat caacttatac catgctattt cctcattatc
     9121 agcacgtatg aaacaataaa tgcgatgact gtatccttgt agtgcttcaa tcagataagc
     9181 acctaaaaaa cctgtagcgc cagtcaatag tgtatttcct agaggtcgat gacttagact
     9241 atcctctaaa atacccaaat tataacgaga cataacaatc ttttgtaatt ccgaaagatt
     9301 atccggtaat gcaactaatg attgttgatt ttggtgcata taattaacaa tctgtcgcac
     9361 ggttttatat tgatataatg tctgcattga aatatgatgg ccaaatcgtt ttaaatgcga
     9421 gacaactaac atcgcctcta atgagttacc accaagttca aagaagtcat catcaacacc
     9481 gacatcattt tgtatcaata cctctccgaa tacatcaaca aatgtctgct caatttcatt
     9541 agatggttcg ctatacactt tattagactg ttgtataggt gatggatttg gcaaacgcgt
     9601 agtatccacc ttgtcattcg tggttaatgg tatacaatcg atatgcgtta tagtcttagg
     9661 aatcatatac ttaggcagat gatcatttaa atattgcttc aaatcctgct ccatttgttg
     9721 ctctccaaca taataagcat tcaatatatc atgcgtatca aagtgactta ctgtgacaac
     9781 acaatcagat ataccacgaa tagctaatat tgcattttca atttcatcaa gttcaatacg
     9841 gtacccgtta actttcactt gtttatctat tcttcctaaa aattcaattt gaccatcaga
     9901 tgtataacgt gctaaatcac cactatgata caactttcct ttaccaaatg gattattttg
     9961 ccatttatca gccattaatt ctggacgatt aatatatcct atcgctaaac tatcacctgc
    10021 aatacacaac tcgcctggca taccaatacc gcataacaaa ccatctgaca taatatacac
    10081 ttggatgtta gataagggtt tgccaattgg aatcgtctca ggtatcaaat caccacattg
    10141 atgtgaccaa tacgatgtga tgactgttga ctcagatggt ccataggcat tgaaatacgt
    10201 gccgcattgc ttctcaatat atttaacaaa ggatgccgta ctagttgccc cgcctgtaat
    10261 caatttttca atataaaagt cttccataac actacacatc tgtaatggaa tcgatgccac
    10321 cgtcacacga tgcttattaa tgaggtgttg taactgtgct ggattaacac gttcctctct
    10381 atctggaatc acaagcgtat gaccatttaa caaacaacaa taaatctcca taactgatgc
    10441 atcaaaaaca atatttgcat gttgcaaaaa tacttcattg tcgcctaatt gcaattcagt
    10501 tgaccatgca tatactaaat tcaacaaatt tcgttgtcgt atggcaaccc ctttaggcat
    10561 cccggtcgta ccagatgtgt aaatagcata catctcatta tctaacatcg ctgtgttttc
    10621 aagttgatcg ccatgtaaat catcatattg ttcattttcc tttgattcaa caaaacattt
    10681 agcattttcc aattgaatga ctggtaatgt cgtttcaatt tcaatgccgt aagacatcac
    10741 tgcagttact ttagcatcct ccaaaattgc gccttgtcgc tttttcggaa aatcaatatc
    10801 gataggtata taagatgcac ctactttaac tgtcgccaac atcgccgcaa tcatttcaaa
    10861 actacgttct gttattaaag caacccgttg acccttgccc acaccatttg ataggagcat
    10921 gtgcgcaatg gcatccacat agttgcgtaa tgtttcatac gtcattgtca aatcattcat
    10981 gactagcgca acatgattac cttgtcgtga gacaacttca ttaaagtaac ttatgataga
    11041 tttatttccc gggacattaa gcattcgatc gttaacatgc gtattgaccc aatttagaag
    11101 ttcctccgtg ccgtttggta tatcacaaat ttgtagtgta tcttgatgct tcaaaatata
    11161 atcaatcata atcatacatt gattacccat gtgacgaact gtttctgagt gatataaatc
    11221 ggtattatac tcgatattga ttgtataatc atcgcaatct tcttcaatga tgaaagataa
    11281 atcaaatttc gccgtcactg atttgggttg aatatgtgtt aatttactat gcccaaaatg
    11341 agcatgattc gtttcattgt tttgtagtac taacatgaca tcaaataatg gattccgtga
    11401 ggcatcatgt gattgatcta agtcatttac taaaaattcg aatgggtatt cttgatgctc
    11461 gtatgcttcc aaactcattt ccttaacctc ttgtaaaaac tgtgtccaca ttttatcaag
    11521 tgacggttgc cctctatata ccaacgtatt agcaaacatg cctagcattt gctccgtgcc
    11581 tttatgcata cgcgcactca tgacactacc gacaacaaca tcatcttttc gagcatatct
    11641 gcttaacaac gtcatgacca cactcataaa gaacataaaa tcagtaattt gatgcttttc
    11701 tacatacttt tgaagtagct gtctcatttg ttgattcatt gtaaatgaca tcattgctcc
    11761 atttgtcgtt ttaatatttg gtctaacata gtctgtcggt aagcttaaaa taggtacttc
    11821 atctttgaat tgagataacc aatattgtct atgtttcgtc atatcacgat gcgacatcca
    11881 ttcactatag tctttatatt gcaatttaag tggtaacaat aatttatgtt gatataatgc
    11941 gttaagatca ttcattaatt gtatattgct cataccatca ttaataatat gatgtgtatc
    12001 tataaacagg tatgcatgta atggacttcg aatgtatctc actctaattt ggcttggctt
    12061 ttccaaatta aaaggtgcca caaattgacg catgatttct tgttcatccg taaaatgcgt
    12121 gttaacttct ttaaagtcag ctacaacatc tgccacaata cgttgtcgaa cctcatcatc
    12181 tacaacaata tattgtgttc gtagaatctc atgtcgcgct atcaaatgct gaactgcttg
    12241 tcgcaattga gctacattaa gttctgatga taaccgccat aaaaaaggta tgttatacac
    12301 cgtatcttta tggtttgatt tccataataa atacatacgc ttttgtgcag agctcaacac
    12361 ataatcatct ttaactatag cttcaggaat cacttcatag ttttgttctt gaaccttagc
    12421 aattgcttgt gctagttcaa atacagttgg cttttgtaat aaatcaccaa tttgtaatcg
    12481 tttcccagta gatgcctcta tccgattcac cactaacgtt gcttttaatg aatggccacc
    12541 taattcaaag aaattatcat gaatacctac ttgattcaca tgtaaaatat ctgcaaaaat
    12601 ttggcatagc aagtgttcgg tatctgtact cggtgctaca taggcatccg tatcgacata
    12661 gtccatgata ggcaatgcct tcttatctaa tttcccatta atagtaatag gaatttgctc
    12721 aatatgcata aaattaactg gtatcatgta ctcaggtaag gtcatacgta attgtgattt
    12781 aatcttatta tgtgataatg tatgcatcgc ttcataataa gcaacgatat actggtcttg
    12841 atcatgattt tgaacaataa caacagcttt attaatacct tgtatacgct cgagcgcatg
    12901 ctcaacctct gacaactcaa tcctaaatcc tcgaatctta acttgtttgt cctttcgata
    12961 taaataatct atgttgccat cgggtaacaa acgtacgata tcaccacttc tatacatcag
    13021 ctgatttata tttgaatctt tgataaattt atctgctgtc aattctggct gatttaaata
    13081 acctgcagct aacccaaagc cacttgtaca taattctcca ggaataccaa cgccacaccg
    13141 acgctcgcct tgcatgatat aaacatgagt accaagaatc ggtttaccaa taggaatacg
    13201 atttggaact ttgttaggta tattatacgt cgttgtaaat gttgtatttt cagttggtcc
    13261 ataaccatta ataatttgag gatgcttcgg tctttgatta agcaaatcca cccacttagc
    13321 attcaatact tctccaccaa ttaataaata ctttaacggt accaatactt ctattcgttc
    13381 actagcaatc tgattaaata atgaggaggt taaccacata gtattgacgt cattttcatt
    13441 gattaattgt tctaacgcta ttggatttaa taattgttct gttttagcaa caatcagttt
    13501 tccaccattt agcaatgcac catatatttc aaatgttgca gcgtcaaagg ctatagttcc
    13561 tgataacaaa atcgtcgtct tttcatttaa tggtacataa tgattttgat ggaccaatcg
    13621 aacaatacct cggtgcggaa ttagtgtccc tttagggtta ccagttgtcc ccgacgtgta
    13681 aataacatac gcatgatctt ctaacgtgtt acatttagaa agattatcaa cttcttccca
    13741 cacaatcgtg tccaactcaa agtgtggaat acagtcatca ttattgttga atactacttg
    13801 atatgtgata acaactttag gcgttgcatc ttttaaaatg tactcctgac gatcacttgg
    13861 atagttcgga tcaattggca cgtaagcccc accagctttc aacacaccta tcatcgctat
    13921 tatcatctca atactttttt cagctatgac agcgacacga tcattaggtt caacaccata
    13981 ctggtttctc aaacgttgtg ctaaatcatt cgcgcgtgca ttcaatgttt gatatgttat
    14041 aaacactccg tcaaattgca cagcgacatg attcggcgtt gcttcaactt gttgctcaaa
    14101 taaggtaaca actgtttgcg catcatctat ctcaggcaaa cttaaattga tatcgtcata
    14161 taattgaata tcactttctg tcatcaaatt aagttcatct acggttgttc gtttatttcc
    14221 attttcttca gtaatttgca aataaatatt ttgaactaag tcactcagcg tctcgattga
    14281 gagcaaatca tatgctgcac tgttatatat aattttgaac ccgtgttgat gtggaaaaat
    14341 ttcaatatcc gctaaagatg tatgtgcatc atgtatttga tgtacatcct caataacatc
    14401 attacaacaa gacatcattt gatgataaca atgaaaaata gtctctaatg aaagtgaaga
    14461 cttcgcgcac tgtaattgcg acatattttg caacacactt ttattaaaat ctgttgtaaa
    14521 acgttgacat acatcttttg catcgaatgt taacgttaat ggcacaatat ttccgtgtaa
    14581 atcatttggt aaatgtgatg gtacatgtat acctaatgtg acatcatgtt gttgactcat
    14641 tatatagtta gctaaataca cactaacagc caacgatgcc agatctatcg atgtcatatc
    14701 atcaatcaaa tacgtttgat ataaagcttg ctcaaatgga tgcttaattg gtaaataact
    14761 atcgatatgg atgtcagagt tattctctag ccgaaaatag tttgagtcta atgctatatg
    14821 caaagcatct tgattgtctt tatcatcatt tctatttaaa tgtgcatgct gtcgagtatt
    14881 gttaataaca gtattgccac gatatgcatt gcataaatca tcaagaaaaa tatcaatttg
    14941 actatcatcg aaaatggaca catgaaaatc taataatata tatgcagcat cagcgaactg
    15001 aaacaattta aacttgaata aaggtgaatc gttaaaatgg taagtactta attcttgctt
    15061 aaaaaaagct tctaaatcat agtttgcgga agaagatgga acttgtttta tctcaataaa
    15121 aggcagaaat tcatgaagta tcattgttaa attgtcatcg gtagtaacat caaaaaaatg
    15181 tcttatagat gcatgttgtt gcacaattgt cgataatgca tacatcattt tagtaacttc
    15241 aacattttta gcgagtttaa cccaatacgc attacggtgt gtcgttgatt ctgtattatt
    15301 tttgtatata cgaaaatatt cctgttgaaa tctcaaatta cccataatca taaaaagtcc
    15361 ttctttcata tcataatact cattacttac tgaaattgca tgatgatatg ataaccgacg
    15421 aaatgttaat taactcgtta tgtaatgatt aatataaaac accattcgca acatatgagc
    15481 gatatattct accctaaaat acatcttgta tcatcgttac aattggtata tttttcaatg
    15541 taaattacat acatattcga taactagcac actacaactc gttaatcact ttctcttgtt
    15601 catatctcat tgcaaattca atattgttgt tacaaaatat ccatgaagca agtttatatt
    15661 aaacaaacaa ctcgcataaa acaattgtta tccttaaatt ttaacaaatt cttaataaat
    15721 ttatctctat tttaattacg accaaattaa taggttttca atataaaaag atgcatagaa
    15781 taaatattta aatcaattca atttgtattc acttgttttt gtcccccgaa tacaccagca
    15841 acaagcatgc tagcaccaat tgttaaaact ataaacatat acagtcccat ttgtaatgtc
    15901 gttaagaaag cacccaacac aatccctaac ctagctagtg tttctgaaaa atgaatgcct
    15961 aatgcattaa ctgcactata tgttcctctt ttagctttag gaataatttt aaaacgttgc
    16021 tctgaaacta taggcgaata aataatttca cctacagtcg caattatcat aaaaacaact
    16081 aataagccaa actgattaag ataggttaga ccactatagc caacaatata aatcagcaaa
    16141 ccagtgatta aagctttttt aaaatctatt ttcaatacaa ctttcgagat tgaatacgtg
    16201 agtaaaatga cgacgaccgt attaatcatt agcaagattg ctaacatctt agcacctgta
    16261 atatcatatg aacctatact tattgtttca aactgatcct ttagtctaat agcaatatat
    16321 gaggagattg aaaattcacc catcatgatg atactgaacc ccgaaatcaa taacatataa
    16381 ttacggtctt tcaaaactaa tttataactg cgaaatatat ttattatttg taatttttga
    16441 taacgacttg catgtctctt gtcatcactt tgctttactt gatttcggtc ttgaggtaac
    16501 caaatatata aaataaagag tacaattaaa aatatacaag ctgctattaa gaaaagtagt
    16561 aacatactgt agccatacat caagccacct aacaatgccc caatagctac cgataagttc
    16621 gtcatccaat agctaatctt gtaaatataa tgttccacgt cttcggtaat tgcatccata
    16681 attaatgtgt ccataactgg aaattgtaat ccccaaacga ttgtaaatat ggcatatgca
    16741 acacaaaaac caataatttg ccacaattga tgtgacccaa atacgcccat gaacacaagc
    16801 attatcacca tcgtcgcttg ataaataagt actagcaact ttttcggaaa aatctcaata
    16861 aggtaaccag atataatgga caatggaaat tttagaacca ctaaaccaac aagatatata
    16921 ccgacaattg attgacttaa catatctgtt aaatatagtg ctataaacgg tataaatgct
    16981 gtcgtaataa ttagctgtaa aaaattgcta atcaatcgta ctttcaaagt cgtacttaaa
    17041 cgtttcattg ataactacac ccctatccac atatatacac actaccattt atataaaatc
    17101 tattatttga tcatacgttg aagcaaagaa tttttagtta tacaaagtta tttaacaaac
    17161 aaatgcgtcc actttacaat gcatcttgca tattattact caaaaaatta tcactatatg
    17221 actatatatt attccaaatg tgtgaaattt atcaacattt gcgtaagtga acacatatgt
    17281 gcgtaaatgt taaatcattg tcctaacata gccgtgacta tacttagatg taatcataaa
    17341 aaaactactg ccatctttac aacagcagta gtgcttatat gattcattta tataaaattg
    17401 aaagcgttaa tcgctagccc agcatcctta ttctatttag ctgtataact tttactagca
    17461 atacgaccac ttgcaacaat aatatcttta tcttggaaag gttcatgatt gaagaaacgc
    17521 tctaaaatat ctttaactcc atcttcaata cgtttttgtg cttctaaagt catacctgaa
    17581 tagtgaaccg tcatagcatt tctaggcatt gttctccatg gatgatcagc aggtgcaggt
    17641 tgtggatacc aaacatcacc agcatatcct tgtaaatgct cagatgctaa cgcttcaact
    17701 aacgcatcgc gatttacaat tttaccacgt gcagtattca ctaaataact gtgttttttc
    17761 atacgactta aaacatcttt atcaaataag ttatcagttt ctggtgttaa tggtgcatga
    17821 attgtaatcg catcacttgt tgaaacaagt tcatcaaagc ttacaaattt agacaattta
    17881 tggtcttgtt gattgattgg atcatagtgt tgtaatgtta cattaaatgg cgctaatctt
    17941 tcagcaacaa gttgtccaat tcgaccaaat ccaaaaatac caattgtttt gtgttgtaat
    18001 tcatgcgcat gattacctac ttgagacaag ttccattcac cttctactga ttgacgatga
    18061 ccttcttcat agtttctaag aagtattaat aaatccataa ccgcatgttc tgccacacta
    18121 actgtattac ttcctgtaac ttcaacgaca ccaatattgt gttcacttgc tgccgctaaa
    18181 tctacatgat cagatcctac acctgctgta attgctaatt tcaagttcgg tgctttttca
    18241 atacgttcac gagtcatata tgcaggataa aatggcgcac taataatcac atccatatct
    18301 ggtaaatgtt tatctaagtc ttcaccatta tctgctaata taatgaactc atgtcctctt
    18361 tcctctaaaa atgtttttaa tcctaatgct tttttagtat taagtaattg attttcttga
    18421 ccttctactg cttctggaaa taacgctacg attttcatat ccatctctcc taaatgataa
    18481 attttgactt ccttaaagta cgtgttgcat gttgcttgtt ttaaaaatat gacaaaaaaa
    18541 cggcaccgtt tgacatacca cccccttgaa ttattgttaa attcaaagcg aagttagcaa
    18601 acagcaccgt gtaccttaca ccagcaacac ttattctatt gacttatcga aaacaaatat
    18661 tgaaatgact tcatcatcat tcaaatcaat atctgtaaat aattttacga acttagcgcc
    18721 tacaagactt tccatctcat ttacatctat ttctttatac atctgtttaa tcttctctgt
    18781 gcgtgtataa tggagcatcg attcattgcg tttgtcgttt aggtaaaaac tctcaacttt
    18841 actcaaaaca cctgtcatat gtgcaatcgc ccaattatct ttaaacgaca ctcgaatact
    18901 attgggtcct ttacccacgt attctttacg ataagatctt actagattcg agaattgttg
    18961 ttctttagta tgcgtatcta tagtattcac cttctttaaa ataagaagtt cattaattgt
    19021 tcacattttc attatacacc ttattaacac tttttcaatg ataacgatta ttattattac
    19081 gaaatgtata ttttcctagt taaaaggata taacaaatta acagaaaatg tttataaact
    19141 aagatttata ttattttaaa aaatgtttca acaaacgcat ctaaacaaaa aatcagctta
    19201 tcacacttac cccttcaact cagcttcaag tttacctaaa tgtcttaatg ttggcgttaa
    19261 tgctgcaaag aagaatcctt cttttaattt acgtacttgt ggtgaatatg gtgtatatgc
    19321 tcttgaacca ccattgacaa cagaagcttg atttacatct aacaataagt agccaatgtc
    19381 cttcttcaat gatattaatt catttaaatg tgaagttaag ttaccgtcat ctaatattgc
    19441 ataatattcc tctctaagtt gacgatagcg ttttttaaaa ccttcaacat catactctaa
    19501 atattgattt attccgtttt gcgcatttga aaatgcatca attaactcta aagaactttt
    19561 aattgagcct aatcctattg gaatttggta agcaataaat tgcgggcgaa tagttgccgc
    19621 aaactgcttc gcatcatgcg tgataatttg tgattgtggc actacgactt gattcaaagt
    19681 aatttgatac gttgctgagc cgtttacccc taaaaaattt gttttttcaa caagcgtgat
    19741 accatcttga ttggcacgta gaatgaacat gacaaattca tctgatgatt catgcttcga
    19801 aatcgcacca aaataatggt cttcttgaat attacttaca gctggcatac gtccactgac
    19861 aaccaattgt ccatcaacat aagtgtgttc aaggttcaac ttttctaaat cattaaatga
    19921 cttcatcgga ttagacaatc cggtagcacc taatatttct ccagataaca attgctgttg
    19981 taagtcatta tttaaatgtg gctgtgtggc attttctaaa tacgttgaaa aagctaattg
    20041 gcaccataaa caaaatcctg ttgtcaaaca agactgcgat actttttcga ttacttcagc
    20101 attttttctc agtgtcgctt caccaaaata gccatctaca aataattgtt gaataaatgt
    20161 tctcggataa tacgtccctt catcaacttc tactaaatga ggatctaatt gttctttgat
    20221 aagcgtttct aaagtcatca tcatctctcc ttattcacca aatcttctaa gtataaactg
    20281 ctcaatataa ctaatgaatc gatcaataat aaaaccaaat aatccgataa agaatattgc
    20341 tgctaaaaca tcttctaagt tcaacatatt tcgtgcatca acgattaaaa aacctaatcc
    20401 cgattgtgca ccaatcattt caccagaaac taaaaatatc caacttgttc ctaccgccat
    20461 atgtatccca gccatgattt gtttaaatgc cccgggaaat aatatattgc gatacaatga
    20521 ccacccagtt aaatttaaat ttgccgctat ttttaaatat tgaggttcaa tgtctctaac
    20581 gcctttaatg gtattgaaca caattgggaa aaaagcagcg ataaaaataa tcgcaatcgc
    20641 tggcaaacta ccaataccaa accatagaac aacaaatggt gcccaagcta tcggagatat
    20701 cggcctaatc aattgaaata gcggttcgat agcgttgtat agccaacgat tccttccaag
    20761 caagaagccc aatggaatag caaccaacaa tgcgacaaca aagcccgcta caaatctcca
    20821 taaactaatt gctaaatatt ggaaaatttc tccagtaaca ataaaagtcc atatactttt
    20881 tcctacaaga gcaggaccag gtaacaatac aggttggtaa tgcccaataa taatgaccat
    20941 ttcccaaatg cctaagaaaa taataaatgt gataataggt aatataattt tattaattgt
    21001 gagacgtgtc atgaacgtga tgcctcctta tacaatgacg gttcaacaaa gtcatcatat
    21061 gcaggtggat taaacaaatg atgttgtttt accaatgtcg taatttcttg atagccggat
    21121 ggcttaattg ttaaatcacc ataggatgtc catgccgctg actgtgttaa aacgtcacga
    21181 ctttgtttaa aatgatgtgt cataatgtct acactttgct tgcgatcatt cattttaaag
    21241 ccagactttt tataatcttg tacaaatgct tgcgctacag ccttgtgttg atcaagcaat
    21301 tcgcctctca gtactagcac acagcaatac gcatcaggta taacgtcatc accatgtttc
    21361 agagtcttac ctttgcctaa cttttccccc agtgcaccga atggttcggc tacagaatac
    21421 cctgtaattc tgtgttcact taatgcggct ggcatttctg ctggcgacat ttcatgatag
    21481 ctaaaatgcc ccggtttaat ctttaattgt tctcgtaatg cttcaagtaa gagataatgt
    21541 gttgaataac gatgcggtat accaaaatgg taatcatcgc cattattatt aaattcattt
    21601 aagtgcatac ctttttgtcc cataatgaca ttgccttcat gatgacccaa tgccacagct
    21661 tttatattcg agcccttctg ctttgatttc atcgcgagct ctattaaagt tgatgcacca
    21721 tcaatacgac cactgtttaa tgcgtccatt aaatctggcc aattattgaa tttaactaat
    21781 tctaatttat atttcggatg attgtgttgt gataataatt ttttagtcat catcaaatta
    21841 gctgaatgtg taatcggcaa atatccaatt ttaatcactt gctgatgttg ggcattttta
    21901 gacggttctt tagacgtcct ttgccaatca catcctgtaa ttataaagat tccaatgatg
    21961 acgattatgc ttaacctttt catcgtcact cactccttat aaataatatt caggttcaac
    22021 ttgatgatga ttcaatgcaa atgtttccat aatgtcatta cgaatcttaa gtaggtggct
    22081 atcattacga ctgcgtggat gtgatgctgt aatttcatat tgagaaataa tattgcaccc
    22141 ttcacctaac agaacaatgc ggtcggaaag ataaatagct tcatcaatat cgtgagtgac
    22201 taagataata gttgatttcg ttttatgttt tagttgtact agttgatcct gaagtttata
    22261 gcgtgtaaat gcatctaatg cacctaatgg ctcatccatc aatataacgt taggcttatg
    22321 cacatgcgct cgacataatg ctacacgttg tttcatacca ccggacaatt gctcgggaaa
    22381 atgctttcct ctgccttcta aatcaactaa tttaagctgt gcgttaatct cttcatcact
    22441 aattttctgt tggaatccaa tcctaatgtt gtcattaatc gttttccatg gcagcagatt
    22501 atgatgttga aatagcatta aacaatctgg agatggctgt tgtttaattt cgttatcaat
    22561 aatgacacga ccagatgatg gctgaataaa tccaccgatg atattgagta aagtagactt
    22621 cccgcaacca cttttcccta taaaagtgac tatttctccc ttgctaatgt ccaaattaaa
    22681 gttatgaatt actttatgtg atccaaagtg atgttgtaat tgttgtattt taatcatgac
    22741 aagccccctt ttaatcccta ccattttaat aagaattaaa tgtatatcta aagggctatg
    22801 ccaaacacag cccttatatc gcgaattgtt atatctatag atgattatgg tttggtatga
    22861 gtgctcttat aacataagca ttattcaatt aacgacgtta tattaatttc agctctacta
    22921 aatttcaata aaatgattgt tttagacaaa acttgcattt agttcattag tttcgtcata
    22981 tcaactactt acatttatag cattgaacta ttctaattaa ctgcaatcga aatccacgcc
    23041 aaacctatat acatatatat catcaacttg ttgtcttatt aaagtttttc gatgccatca
    23101 atcggtgtat caactgcttt gcgcgcgcga cgtaccgctt cttcatcagg tgcgttgtat
    23161 aaacaaatac atttagacat atcttcacat acatatgtgc gtttaaattc aacttctggc
    23221 acttcttcat aatgaacaga atttttcttt ttacgtgcta aatattgatc catcgtaatg
    23281 ccttccggaa tgttccaagt tacaaggtaa tcaacatgtg catcaccatt ttttacttca
    23341 tctaaatctt taccaactaa gcgtacttct ttgactaatt gaatgctaat acctgcttct
    23401 gttaaaaatt gtttagcttc tgcttcgtca tttgcttcca caataaaata accatgagtt
    23461 aaattttcag tagcttgtac ttcaattaat gttggaattg tcttcgtaga tagtgatgat
    23521 gcctttgctt ctagttcttc tttcgttgat gcaaaatcaa gattgttagc ttctaataaa
    23581 aataatgtca ttaataagac ctcctataac aatggttgaa aaaaatatta ctataattct
    23641 tatcggatta caagtatatt gagcgatttt tttaaatatt tagataaaat tctttaaaat
    23701 atattttcaa agagtgtaaa aataatgtta gggtataagt aaataaagat atatataggg
    23761 ggctatcatt atggataaag aatctgtcgt agcaagttta gcaagaaaca aaaagattgc
    23821 tgtagaaaca atgaccggtc aaaggtacat catagaacgc attttacata caaatgatga
    23881 aaaacatatt catattttga aacctaaaga tgtcgtgtta gatgtaaata ccatcaaaga
    23941 catcgacgaa aatcatttag atgatgcaac ataagtataa aacgaaacgt gcgcctatga
    24001 catagattaa tgttttaggc gcaacaatgt tttatttatg ttaatttgtc gaagtatggc
    24061 tcatctaatg gattcaattg taagttttca atttgcaacg ttgaatgtgc aatgccatat
    24121 ttttctttca acaatgatga tacttgatca atcgcttgat aatcatcgcc ctcatatttt
    24181 ttatctaaca caacatgggc acttaatgaa taatgttctg ttgtaatact ccacaaatga
    24241 aattcatgta catctaatat gccatctatg ttttttatat ctgccataat ttgatcagta
    24301 tccaaatgtt gaggcacact ttccattaaa attaaccacg cattacgcgt aattttataa
    24361 ccaccacgta aaatgatgag tgaaattaca atactaatga ttgggtcaat gatgcgccat
    24421 cctgtaaagt agatcaatac aactgcaacg atgacaccaa tagagttcaa taagtctccc
    24481 atgaaatgcc ataatgcact ttgaatattg atattgtctt cttgttttaa agaccttaca
    24541 aggataacag tcaaaataat attgacgagt aaaccaatac tagcaatcat aaacataatg
    24601 ccactttcaa tgggttgcgg ataaataata cgtacaatag cttcatataa aatccagatt
    24661 gaaattacaa ttaatgctaa accatttaaa aatgcagcta atatctcaaa tcttaaatat
    24721 ccgaatgtgt agcgtgcagt tggcttctta ctagcaaaat aaattgccaa cattgacaaa
    24781 ccgagtgcta atacatcact aagcatatga aatgagtctg acagcaatgc taatgaatta
    24841 gatactaaac ctccaacaaa ttcaatcact gtaaataata gtgtgatgat aagtgatgcc
    24901 catagcgtct ttttagaact ttgttgaatt ttttgatgat cgacatgatg aaaatatttg
    24961 tatctgttta tcgtattcgc ctttttcccg ttcatagttt gccactccta aataaatgcg
    25021 tcgtatttaa tattgtcgct tttaaaaata attctttata gcttctcagg ttagtattaa
    25081 tgcttccgta attaaccata atttatacat catcgttctt tacatgacaa ctcattaatc
    25141 tttacccttt atacctttat gtaatatcac cttggaaaaa cttttacagc acactcgcgc
    25201 tttgctttat ccctatcata tagaaatttt taaacatttc cgatatgata gggataatta
    25261 cataaaaatg acaaaaaagt ttctcaaatt tattgtttag tttcattaag ttcaaaaaat
    25321 cgcaataact ttaatgattg ttccgcatat taaaacaaac gatgactttg catattgaac
    25381 ttcatatttc taaatcacca taattcataa acaaatctgt ccttataata gccgcatcgc
    25441 aaattagcat aagtaaaaac agcagtaaga tgatttgcaa ttaaaaacca tcctactgct
    25501 attattttag tcattatatc ctagtatcat tatgcaagtt cctaaatatt ttgtttcgct
    25561 taaaatctaa acaactccgt aaacatgcta agacttaccg ctagactgcc tacaaacacg
    25621 cttcagaaac gatatttatt ctagtacaaa ccttttaaag cattgcttgt atcaatataa
    25681 atatttttaa cttgttgata gttacttaac gcacctttat aagtttctcg accgatacct
    25741 gattttttat aaccaccaaa tggtgcgcct tctggtactt ggttgtaagt attaatccaa
    25801 atacgtcctg tacgaacagc tttagcaata tttaatgcac gtgtgatatt ttgagaaaat
    25861 acgccgccag ctaaaccata ctctgaatca ttagctatat caattgcttc ttgatcgtcc
    25921 ttcactttaa tcactgttaa cactggtcca aatatttctt cttgtgctaa tttgtgatga
    25981 ttgtctggca cagcaattaa tgtcggctca aagaagaatc ctttatctaa cccattttca
    26041 gttaagcgat gtccacctgc taaaatttgt gcatctgatt ctttagctgc atcaatatat
    26101 gattgaattt tatctaattg atctttacca gtttgactac ccatttgtgt agcttcatct
    26161 tgtggatttc caactttaat atttgaaaat gcctcttgta aacgtggcac caattgatca
    26221 taaatttttt catgaactaa taatcgagaa cctgcactac atacttcacc ttggttgaat
    26281 aaaataccta actgaatacc ttcaactgca aggtctaaat tagcatcatc taatatgata
    26341 ttggcgcttt taccaccaag ctctaatgta gcgggtacta gatgttttgc tgcagcttcg
    26401 gcaacttgat aacctacatc agttgagccc gtaaatgata atttatctac accatcatga
    26461 ttgaaaattg cattacctga ttctgaacct ttacccgtta gtatattgac aacaccttta
    26521 ggtaatacct cttggaaaat tttagcaact tccaataaac ttaatggtgt tgaagacgaa
    26581 ggttgaatca caattgtatt acctgcagca atggctggcg caatcttcca tgcagctaat
    26641 agcattggga agttccaagc aacaacagca cctacgacgc caatcggctc atgtcgtacg
    26701 atactcattg tgtctttatc gatatcattc actgtacctt cttctgtttc aataacactt
    26761 gcgaaataat ggaaatgtct tgcagcaaat ggaatatcaa ttgctgttgt ctcacgaatc
    26821 ggtttaccat tatttaatgt ttcaatcatt gcaattttat ctttttgtgc cattaattta
    26881 tcaccaatat cacgcaacat ttgtgcacgt tctgatttag aagttaatga ccatgattca
    26941 aatgcctctt gcgccacttc gactgcatga tcgacatctt tatcttttgc tcttgtaata
    27001 tgtgatagtg tttctccagt tgctggattg gtcacttcga ttgtttcgtc actgctacct
    27061 ttaacaaatt ccccattgat aaataaacca taattctctg caatataatc tcgaacgttt
    27121 actgccatat tatctctcct ttgtttattg gctataatat aaatctctta gcatcatatt
    27181 taataatgct tttaaacgat atgattcaca ttatatagta agcgtttaca ttttataaaa
    27241 taatcagctc acagaagtct catttttaaa acttttgcgt cgtttttgca cataaaaata
    27301 atcttatttt cgaaaacata ttgcactttt caaaaataaa acacataata taactataat
    27361 acttgagaat aattctcatt aaatagtttg gttgttatga aagtcgcgct taaaatgcgt
    27421 taatgggaca aggataattc aacaacgttt cattttactg agtcattgca cttatcatac
    27481 acattatatt tagcatgagt tatattacta aaacaatatt acatgcgcat tagtactaga
    27541 atggagtttt ttcaatgcga cttattttaa tcgtcattgg cttaatattt acagcgcttg
    27601 gtattgcagg tgccgtatta cctttactgc caacgacccc ttttttactc gtagcagttt
    27661 tttgttttgc tcgaagttca gatcgctttt acaattggct tattaatcaa aaaatttata
    27721 aagaatatgt agaaaacttt tatttacatc gaggctacac gctacaacag aaaattaaaa
    27781 ttttaattag cttatacatt gtaataggtt tttcaattta tatggtggat gttcttgcag
    27841 tccgtatagg attaatcata atggttatca tacaaaccgc tgtactcttt acatttgtaa
    27901 aaacattacc caaatcaaat aataaaatag aggagtgatt gcccatgttt atggcagaaa
    27961 atagattaca attacaaaaa ggcagtgcgg aagaaacgat tgaacgtttt tacaatagac
    28021 aaggtattga aactattgaa ggcttccaac aaatgtttgt cactaaaaca ttaaataccg
    28081 aggatacaga cgaagttaaa atcttaacta tttgggaatc tgaagatagc tttaataatt
    28141 ggttgaattc cgatgtattt aaagaagctc ataaaaatgt acgtttaaaa agtgatgacg
    28201 atggacagca aagtccaata ttatcaaata aagtattcaa atatgatatt ggctaccact
    28261 atcaaaaata aacatattaa aaacccctag tcaccgctgg ttgtctaaac caacttcatg
    28321 attaggggtt tttattattt gcgacgtaaa ggtacgaatt catccggctt gtctgaacgc
    28381 aatccaaaat aatattctat tgcttcacaa atacgtcgtg atgcttgtcc atcaccatat
    28441 ggattattcg cttgactcat acgttgatac gctttatcat cctcaatcaa ttgtttcaca
    28501 tttcgaacaa tattgtcata atctgtgcca attactctcg atgttcccgc ctctacgcct
    28561 tcgggacgct ctgtatgatt ccttaatacc aacacaggtt ttccaaatgt aggagcctcc
    28621 tcttgaatac caccagaatc tgtcagcacg aggtacgatt gatttgtaaa attatggaac
    28681 tcaatcgcat ctaatggctc aattaattca atccgatttc tcccagataa atatttttcg
    28741 gcaatcgctc ttacctttgg attacgatgc attggataaa tgaagacaac atctttatat
    28801 tcatctgcca aatctcttac tgctttaaaa atctgatgca tcggttctcc aatattttca
    28861 cgacgatgcg ctgttagtaa aataactttc ttgcctttat gtttattaat aatcgttgaa
    28921 acaaaatcat tttgaactgt tgttgacaat gcgtcaataa ctgtatttcc agtaataaag
    28981 atcctctctt tgtctttgtt ttcaaaaagt aaattttcag ctgcaattac tgttggcgca
    29041 aaattcaatt cagcaatatt acttaccatg actcgattta actcttcagg aaatggtgag
    29101 tatttctgat gagttcgaag tccagcttct acatgtccga ccggaatttg atgataaaat
    29161 gctgccaaac ttcctacaaa agtcgttgta gtatcaccat gtactaaaat catatccggc
    29221 tgttcttcat taatgatgct atcaagttta gcaagcgcat tcgccgtaag gcccgctaac
    29281 gtttgttgat cttgcataat atttaaatca tgatcagcat gaatatcaaa tatacttaac
    29341 acactatcta acatatctct atgttgtgct gtaatcacaa tgttcgcttc aaagttccca
    29401 ttatgatcaa tttcttttac taatggtgcc atttttattg cttcgggtct cgtaccgaaa
    29461 ataaccataa tttttttcat aacattatcc tctctgaagt tcaaacacat gacagcttat
    29521 ctttccttaa tcatgtatgc cctagtttga cacattttat ttgtcgataa aattaaatat
    29581 attgccataa ttataatacg atacatcttc aaatgatgat ttcacaacat tttttgtatc
    29641 aaaaatcact ttatgcttca ttttatcaaa atgactgtcc gataaatttt taaattctga
    29701 gtggtcactt aaaatcaata ctagcgatgc gtctttgaca gcatgtgaca tatcatgttc
    29761 cacaaaatct aattcaacat gtggatcata agcacatact tctatgtctg gttcttgatt
    29821 taatagctca taaatatcaa atgctggtga ttctcttata tcatcaacat cacctttata
    29881 agttaaacca aatactgtga ctttattccc gctcaacgct ttgatgattt gcttcgttgt
    29941 atcaacaaca taggccggca ttgaattatt aatttcacgc ccagtttgaa ttaactttgc
    30001 attttcaggg tctttagcaa taataaagta cggatcaaca gctaaacaat gaccgcctac
    30061 acctggacca ggttgatgga tattaacacg cggatgtttg tttgccattt caatcacatc
    30121 taatacatta atatttaagt tgttgcaaat ttttgttaat tcattagcta aagaaatgtt
    30181 catgtctcta tatgtgtttt ccattagctt actcatttca gcagtacgtg catctgcttc
    30241 aatcatttct ccctgaacga atgtgcgata gacacgttta cccgcttcaa tacaagcttt
    30301 agtcacaccg ccaatgatac gattgttatg aactaattct tctaaaattt ttcctggcag
    30361 tacacgttct ggacaatgca ctaaataaat atcttcacct attgtaaacc ctaaattttc
    30421 aatgactggt tttacaaaat catccatcgt tttaggcgca attgtcgact ctacaataat
    30481 ggtatttcct ttttctaaaa atggtaaaat actatctaat gcacgcataa ctagcgaaat
    30541 gtcacatgac cggtactgat catcattatt cggtgtcgga acggcaatga taaaaacatc
    30601 agatgcttct ggcgttgtag ataccttcaa ttttcccgat gacagtacct cttcataaac
    30661 ctcttgtagt ccaggttctt caatactaat ttgaccactt tgtaacttat caatcgtttg
    30721 ctgattaata tcaacaccaa gcacatcgac gccatgcttt gcaaacataa ttgatgttgg
    30781 taaaccaata taacctaagc caactactgt taacttcata ctataacctc caaataaaac
    30841 gatttgtatg ttgattttat ttaatagatt acttatgcct tatctttggt tgtcgtttca
    30901 tccatgatgt ccgcaataac aagtgacatt tttccaggaa taacttcaag cgcattatta
    30961 ttttcatata atgtattgct gtatgttaaa ttgccgaata attttctaaa gaccgacaac
    31021 ttattaaaat acttatttag cattgaaggc atgttgatca ataccgtttt acgatgtgat
    31081 tggcgacgta tttcatacat tactgacgat gtatcaaagt aaaaactatc ttgaggatga
    31141 tacacgcctg tcacttctaa tgatattaat tgatcaataa atgctgtcag atgtttaata
    31201 tataatgtac tacgctgatt gttaatattt ggaatgattg gcaatcgctt tgacaattgc
    31261 attaaccgtt ggaaatttcc tggacaatgt gcaccataaa tcattggtgg tctcacaatt
    31321 gctactttaa acgaatcact aatcaactct tgtaatgctt gttcagcgaa ctttttggaa
    31381 ataccataat tggccgtagg gttcattggt gtttgtgtat caatttgatc tgatttacca
    31441 acctgacctt cttttccata aactgccata gtactcataa aaataaattg tttaacgtct
    31501 tcagctttag ctttttgtgc caattgcttc gtaagcaaca tattcacttg catataatca
    31561 gatagccttg cttgaggtga attgttgtga accaaagctg ctgtatgaat taaaacatca
    31621 taacctttga acgaggtcga cttccataat tgattcctaa cattaatttg atccacttga
    31681 tgtccttgtt caataagctt atcttttaaa gcattaccga tatatccatg tacgcctgta
    31741 attaaaatat tttttctcat tagtgatgca caccttctga agtaacgata tttttaattg
    31801 ttttatatat gatatacata tcaagcatca tagattgatg tgttaagtaa taatgatcat
    31861 acgctacttt ttgatcatca gtaatatcat ctctccccat cacttgagct agccctgtta
    31921 cacctggtct aatcgtatgc acgttcgctt ttgtacgttt ttcgattaat tcgtattgat
    31981 tataaagcgc tggtctagga cctacaattg acatttctcc ttttaaaaca ttcaataatt
    32041 gtggcaattc atcaatagag gtcttacgaa tgaccttccc tgtctttgtt atatacgatg
    32101 ttgaatccat taaatcagtt gcaacattag gtgtgtctat tttcattgat ctaaacttat
    32161 aaatattaaa caattcatta ttaatcgtcg gtcttttttg tttgaaaatg gctggtccag
    32221 gtgattccat tttaattagt aatgctgtaa ttaacagaat cggacttaaa actactaaac
    32281 catatattga actcactaca tcgaataatc gcttcataat tatctcctca cttcaacaga
    32341 ttgtaatact tcatagtatt aatttctctc gtatatttat ctttaaacaa ttgatgtgcg
    32401 cgctcaccca tctcgtgcaa ttccttactc gagtgagtgt tgatgaagtt ataaatggca
    32461 tgtgcatcgc cattatcaat ttggataccc gcatcatatt gttcaacatg ttgaacgata
    32521 tcagattgct tatccatgat gagtaccaat ggcttcttag ctgcaaggta gccataattc
    32581 ttgctcggca cgcctaaacc gacgccttct ttaatcagcg atgcaataca tacatccgca
    32641 atttttaata cgtcagcata gtctgtacct gttaaaaact caaacatttt aacattttca
    32701 atacgatggt cttctattgc cgttttgaca tctgcaaatt tcttaccatg accacaaagt
    32761 attgttaacg tttgaggctg atccttattt aattttaaaa atgagataag tgtctccata
    32821 tcctgtaact gccccatatt accgctatac aataatattt tgtcgtattg ctcacggtaa
    32881 gttttaaatg tgtcattata gatatgattg tcttgtaact gacgcatgtc ataccagtta
    32941 ggaatcacat gaatattgtc aggattttta gaaatttgat gatttactaa atagtttttc
    33001 atttccgtac caaggacaat gacattttca gcattcttgt agacatgtct attaatgtaa
    33061 cgcatcagtt tatcaatcat gctacctgga cgagttgcac ctgtcttaat cgcattatca
    33121 ggtgctatat catacaccac aaaagaatat tttttcttaa gcagtctgtg t
//
LOCUS       DAPIDM010000015        32451 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000015 DAPIDM010000000
VERSION     DAPIDM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.015"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..155
                     /locus_tag="RJE42_000618"
     CDS             <1..155
                     /locus_tag="RJE42_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HDV5876070.1"
                     /translation="EIYIINNTKNFIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISE
                     FDNEDI"
     gene            complement(242..391)
                     /locus_tag="RJE42_000619"
     CDS             complement(242..391)
                     /locus_tag="RJE42_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876071.1"
                     /translation="MLLILELRFWNSEFGFGIRHYNEWKILIIACKIPWFCSDFHLMN
                     CVLFE"
     gene            complement(428..1168)
                     /locus_tag="RJE42_000620"
     CDS             complement(428..1168)
                     /locus_tag="RJE42_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV5876072.1"
                     /translation="MFNLENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDAPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQYVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLITE"
     gene            1374..2165
                     /locus_tag="RJE42_000621"
     CDS             1374..2165
                     /locus_tag="RJE42_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV5876073.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2533..2961
                     /gene="lacA"
                     /locus_tag="RJE42_000622"
     CDS             2533..2961
                     /gene="lacA"
                     /locus_tag="RJE42_000622"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDV5876074.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2977..3492
                     /gene="lacB"
                     /locus_tag="RJE42_000623"
     CDS             2977..3492
                     /gene="lacB"
                     /locus_tag="RJE42_000623"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDV5876075.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3505..4437
                     /gene="lacC"
                     /locus_tag="RJE42_000624"
     CDS             3505..4437
                     /gene="lacC"
                     /locus_tag="RJE42_000624"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDV5876076.1"
                     /translation="MILTLTLNPSVDISYPITALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPKISVLNPVGSGDSTVAGITSAILNHENDQDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4441..5421
                     /gene="lacD"
                     /locus_tag="RJE42_000625"
     CDS             4441..5421
                     /gene="lacD"
                     /locus_tag="RJE42_000625"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDV5876077.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDASTHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5443..5757
                     /locus_tag="RJE42_000626"
     CDS             5443..5757
                     /locus_tag="RJE42_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV5876078.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     GL"
     gene            5760..7472
                     /locus_tag="RJE42_000627"
     CDS             5760..7472
                     /locus_tag="RJE42_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV5876079.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7490..8902
                     /gene="lacG"
                     /locus_tag="RJE42_000628"
     CDS             7490..8902
                     /gene="lacG"
                     /locus_tag="RJE42_000628"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDV5876080.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9168..10037
                     /locus_tag="RJE42_000629"
     CDS             9168..10037
                     /locus_tag="RJE42_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5876081.1"
                     /translation="MKKIFVISTTLLAVAIIIGTIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLSKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGESAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10357..11358
                     /locus_tag="RJE42_000630"
     CDS             10357..11358
                     /locus_tag="RJE42_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDV5876082.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEDIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHIVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQANQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAESLAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPRAFKNLFTGDNFGKQVIKVTE"
     gene            11593..12600
                     /locus_tag="RJE42_000631"
     CDS             11593..12600
                     /locus_tag="RJE42_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDV5876083.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIILNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            12908..14470
                     /locus_tag="RJE42_000632"
     CDS             12908..14470
                     /locus_tag="RJE42_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDV5876084.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITSFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYASITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPAVVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDV
                     LEYRSSKHIDEN"
     gene            14645..15181
                     /gene="amaP"
                     /locus_tag="RJE42_000633"
     CDS             14645..15181
                     /gene="amaP"
                     /locus_tag="RJE42_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDV5876085.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRADIKKNVEHFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15194..15433
                     /locus_tag="RJE42_000634"
     CDS             15194..15433
                     /locus_tag="RJE42_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDV5876086.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15496..16005
                     /locus_tag="RJE42_000635"
     CDS             15496..16005
                     /locus_tag="RJE42_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDV5876087.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(16166..18142)
                     /gene="sfaD"
                     /locus_tag="RJE42_000636"
                     /gene_synonym="sfnaD"
     CDS             complement(16166..18142)
                     /gene="sfaD"
                     /locus_tag="RJE42_000636"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDV5876088.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDNITNMITRVHHPNEILECILIEEPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASMSNFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIAIFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASKDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            18244..19437
                     /gene="sfaA"
                     /locus_tag="RJE42_000637"
     CDS             18244..19437
                     /gene="sfaA"
                     /locus_tag="RJE42_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDV5876089.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDAARYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATIVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYLINRKLKLKLEES
                     NHGIS"
     gene            19424..21181
                     /gene="sfaB"
                     /locus_tag="RJE42_000638"
     CDS             19424..21181
                     /gene="sfaB"
                     /locus_tag="RJE42_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDV5876090.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIMTLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPIDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            21188..22258
                     /gene="sfaC"
                     /locus_tag="RJE42_000639"
     CDS             21188..22258
                     /gene="sfaC"
                     /locus_tag="RJE42_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDV5876091.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     HINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFIQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(22359..22484)
                     /locus_tag="RJE42_000640"
     CDS             complement(22359..22484)
                     /locus_tag="RJE42_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876092.1"
                     /translation="MYHGYIIFELIQLTPFSMTVISLIQLILPIAVLCDELLHLI"
     gene            22651..23634
                     /locus_tag="RJE42_000641"
     CDS             22651..23634
                     /locus_tag="RJE42_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5876093.1"
                     /translation="MRGLKTFSILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNIDAFKTI
                     AKALDKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDNAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            23646..24677
                     /locus_tag="RJE42_000642"
     CDS             23646..24677
                     /locus_tag="RJE42_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV5876094.1"
                     /translation="MAIKELSSQSAIDHKRKRRTTLTYMVSVCFLFICVYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMLAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIA
                     TVFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSIAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            24674..25642
                     /locus_tag="RJE42_000643"
     CDS             24674..25642
                     /locus_tag="RJE42_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDV5876095.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            25878..27248
                     /locus_tag="RJE42_000644"
     CDS             25878..27248
                     /locus_tag="RJE42_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDV5876096.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLLHNAFNVRIGW
                     LILRLLAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGIVDFFKSGLKTVVDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(27381..27641)
                     /locus_tag="RJE42_000645"
     CDS             complement(27381..27641)
                     /locus_tag="RJE42_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876097.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            27818..28333
                     /locus_tag="RJE42_000646"
     CDS             27818..28333
                     /locus_tag="RJE42_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV5876098.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            28377..29564
                     /locus_tag="RJE42_000647"
     CDS             28377..29564
                     /locus_tag="RJE42_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDV5876099.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQAKQLKELHRQTGHMIQWYIMTSDINHEETLAYF
                     ESHNYFGYDQEAIHFFKQDNIVALSEAGQLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEALRRM
                     GLI"
     gene            29583..30266
                     /locus_tag="RJE42_000648"
     CDS             29583..30266
                     /locus_tag="RJE42_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV5876100.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIVNGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTIVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            30439..31782
                     /locus_tag="RJE42_000649"
     CDS             30439..31782
                     /locus_tag="RJE42_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876101.1"
                     /translation="MRLKSIVTVIALILIMFMAAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLTRFKIIYIAIAGLTLFTVGSLMSGLSTHFSMLIISRV
                     IQGFGSGVMMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVVWTFHFPEEETVAKSKFDTKGITLFYIFIGLIMFALLNQQH
                     LYLNIIGFVLAILVALRLFNVEKKVSSPFLPVAEFNRMITLVFITDLLTAVCLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHHIEAKLSRKVIYLSSFTLLLLS
                     SIIIAFGIKLPILIACVLILSGLSFGYIYTKDSVIVQEETSPIQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYALKSGLFGANLHNILGVVSLISVGLIVVWLIFYREAASQTKE"
     gene            31904..32377
                     /gene="sepA"
                     /locus_tag="RJE42_000650"
     CDS             31904..32377
                     /gene="sepA"
                     /locus_tag="RJE42_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDV5876102.1"
                     /translation="MVMNYLKHKFYNLLTTIIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIVFAVEHLMDYFRKMLPENAYFRGTTF
                     HLISYMVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
BASE COUNT        11074 a         4594 c         6027 g        10756 t
ORIGIN      
        1 ctgaaatata tataattaac aatactaaaa actttatata tcatttatat gatgatagag
       61 gtctatggct agcttttaat aataatgaag attatgcaag atattctgaa aaatataatg
      121 atttaatatc ggaatttgat aacgaagata tctagttttt taatattcgc agctattagt
      181 tctttactta ttttatgttt atttttaatc ttacaaatga caaaccatta aaagtaacat
      241 cctattcaaa taaaacacaa ttcatcaaat gaaaatcgct acaaaaccaa ggtattttgc
      301 acgcaataat taaaattttc cactcattat aatgtcgaat tccaaaacca aactccgaat
      361 tccaaaaacg caactccaaa attaaaagca tctccctgtt actcgggaac tgctttttac
      421 gtactgatta ctctgttatt aatgatttta caacggaaac catgtcgtca tgtatgacca
      481 aagtagcgtc gctatcataa ggtgttcgat ctttattgat aataattaaa ttgtcgcctt
      541 taaaatttga tattaatcct gcggcaggtt gtacaacgag tgatgaacct agtacaacaa
      601 gggtgtcagc atgttcaatt ttatttaatg cccgtatgat ggtaggttga tctaacattt
      661 caccgtataa tacgatgtcc ggtcgaatgg caccaccaca attatcacaa tgtttcaaag
      721 ttctatcaat gacatctgac ttcgtataag atttatgaca tgcattacaa taaaaacgat
      781 ttaacgtgcc atgtaattca tcaacatatt gacttccagc gtctgagtgc aaaccatcga
      841 tattttgcgt gatgacacct aaagattgtt gattacgttc taattttgca atccaatcat
      901 gaacgatatt gggcatcgta tcgacaaata gtaagcgctt atggcagaaa ttgataaaac
      961 cttcaggggc atcttctaaa taatcacggc ttaacaagta ttctggtgaa agcccatctt
     1021 ttgaaatttc atcaaataag ccacccattg aacggaaatc tggaacacca cttgcgacag
     1081 atacaccagc acctgtaaaa aatgtaatac gattcgaact atctataata tgttttagtg
     1141 tctctaaatc attttccaaa ttaaacaccc catcaaaata atgaatgatt tataacgtct
     1201 tttgctttta ttatagtata ttcattttac gattatcaaa ggcgcagttt aaacagttct
     1261 ttttaaaaat gaattataca tataatcgaa cagttaaatt atattagttt ttacgcaatt
     1321 tttattatag aataagcatg tatattaaaa tggatgtcta aacataaagg ataatgaccc
     1381 atcgcctaaa taaaggaggc aattttacat tgaataaaca tgaacgtttg gacgaaattg
     1441 ctaaactagt gaataaaaag ggcacgataa gaacgaatga aatcgtcgaa ggtttaaatg
     1501 tgtctgatat gacagttcga agagatttaa ttgaattgga aaataaaggg attttaacga
     1561 agattcatgg tggtgcacgc agtaattcaa cttttcaata taaagaaata tcgcataaag
     1621 aaaaacatac ccgtcaaatt gcagaaaaac gatttatagc tagaaaagct gcatcattaa
     1681 ttgaagatgg agatactttg tttttcggac cgggaacaac agtcgaacta ttagcagaag
     1741 aagtcaatca tcatacgctt acaattataa cgaattgttt gccggtgtat aaaattttgt
     1801 tagaaaaaca aacagcacat tttcgtgtct atttaattgg tggtgaaatg cgccatatta
     1861 cagaagcgtt tgtaggtgaa atggcgaatg ctatgttgga aaaactaaga tttagcaaga
     1921 tgttctttag tagtaatgca gtaaataaag gtgctgtgat gacatctaca ttagatgaag
     1981 cctatacgca acaattggca ctaagtaatt caattgaaaa atacttgcta attgatcata
     2041 cgaaagttgg caaagaagat tttacatcat tttgtcagtt aaacgaattg actgcagtgg
     2101 ttatggatta tgaagatgaa gaaaaagtag aaacgattaa aacatatatt gaagtagtcg
     2161 actaaattaa aaatataact gtattgttaa aatatgtgtc tatatgctca taagtgaatg
     2221 tgaatttgca ttcatttatg ggttattttt tatgcaaatt tatgtaaacg ggaaccccga
     2281 caaagtttta ctacaatttc agacgaatac ctaattaaaa caacaccaca atatgaatgt
     2341 tcacttattg tttgaaaata tgtcataaac aaacagaatg taaacatatt gtgttgattt
     2401 attgtttgtt tatgtttata atttaaacgt aatcaaattg aaagcctttt ctcaaaattt
     2461 tcataaccta caaataaaca tgtcatattt taagaaaggt atttcaaaat taaagtaaaa
     2521 aggagtctta ttatggcgat tattattggt tcagatgaag ctggcaaacg attaaaagaa
     2581 gtcatcaaat catacttatt agacaataaa tatgatgttg ttgacgtaac agaaggacag
     2641 gaagttgact ttgttgatgc aactttggct gtagcaaaag atgttcaaag tcaagaaggt
     2701 aacttaggta ttgttatcga tgcatttggt gcaggtagct ttatggttgc tacaaaaatc
     2761 aaaggcatga ttgctgctga agtatcagat gaacgctctg gatatatgac tagaggccat
     2821 aacaattcaa gaatgatcac tatgggatct gaaattgttg gagacacgtt agcgaaaaat
     2881 gtcgttaaag gttttgttga aggtaaatac gatggtggta gacaccaaat ccgcgtagat
     2941 atgcttaata aaatgtgcta aggaggaaaa attactatga agattgcatt aggatgcgac
     3001 catattgtta cagatacaaa aatgcgtgta tctgaatttt taaaatcaaa aggacatgaa
     3061 gtcattgacg taggaacata cgatttcaca agaacacatt atccaatttt tggtaaaaaa
     3121 gttggagaac aagttgttag cgggaatgca gacttaggtg tttgtatttg tggaacaggt
     3181 gttggtatta acaatgctgt aaataaagta cctggtgttc gttcagcact agtacgtgat
     3241 atgacatcag cgttatatgc taaagaagaa ttaaatgcga acgtcattgg ctttggtgga
     3301 cgtattattg gtgagttatt aatgtgcgat attatcgatg catttattaa cgctgaatat
     3361 aaaccaactg aagagaacaa aaaattaatc gctaaaatta aacatttaga aacaagcaat
     3421 gcagatcaag ctgatccaca tttctttgat gaattcttag aaaaatggga cagaggcgaa
     3481 taccacgatt aagggacgac acatatgatt ttaactttaa cattaaatcc atccgttgat
     3541 atttcatacc caataacagc attgaaatta gatgatgtta atcgtgttca agaagtaagt
     3601 aaaacagctg gtggtaaagg tttaaatgta acaagagtac ttgcgcaagt aggtgagcca
     3661 gtccttgcga gtggttttat tggcggtgaa ttaggtcaat ttattgctaa aaaattagat
     3721 catgccgaca tcaagcatgc attttataac attaaaggtg aaacacgaaa ctgcattgca
     3781 attttacatg aaggacaaca aacagaaatt ttagagcagg gaccggagat tgataatcaa
     3841 gaggctgcag gatttattaa acattttgag caattactag aaaaagttga agcagttgct
     3901 atttcaggta gcttaccaaa aggattaaat caagattatt atgcacaaat cattgaacga
     3961 tgccaaaaca aaggtgtgcc tgtgatatta gactgctcgg gtgcaacttt gcaaacagtt
     4021 ttggaaaatc catataaacc aacagtcatt aaaccaaaca tttctgaatt atatcagttg
     4081 cttaaccagc cacttgatga aagtttagaa agtttgaaac aggcagtgtc acaaccctta
     4141 ttcgaaggga ttgagtggat tattgtgtct ttaggtgcac aaggtgcgtt cgctaagcac
     4201 aatcatacat tctatagagt gaatattccg aaaataagcg tattaaatcc ggttggttca
     4261 ggtgattcta cggttgctgg aattacttca gcaattctta atcatgaaaa cgaccaagat
     4321 ttactaaaaa aagccaatac tttaggcatg ctgaatgcgc aagaagcaca aacaggttat
     4381 gtcaatttaa ataattatga tgatttattc aatcaaattg aagttttaga ggtgtaaaaa
     4441 atgtcaaaat cgaatcaaaa aatcgcatca attgaacaat taagtaataa cgaaggtatc
     4501 atttctgcat tagcatttga tcaacgtggt gctttaaagc gcatgatggc aaaacatcaa
     4561 actgaagagc caactgtagc tcaaattgaa caattaaaag tattagttgc ggaagaatta
     4621 acacaatatg catcttcaat tttattagat cctgaatatg gtttaccagc ttctgatgca
     4681 cgtaacaaag attgtggatt attacttgct tatgaaaaaa ctggatatga cgtaaacgct
     4741 aaaggtcgtt tacctgactg tttagtagag tggtctgcaa aacgtttaaa agaacaaggt
     4801 gcaaatgctg ttaaattctt actttactat gatgttgatg atgctgaaga aattaacatt
     4861 caaaagaaag catatattga aagaatcggt tcagaatgtg ttgcagaaga tattccattc
     4921 tttttagaag tattaacata cgatgacaac attcctgata acggtagtgt tgaattcgca
     4981 aaagttaagc ctagaaaggt taacgaagca atgaaattgt tctcagaacc acgctttaac
     5041 gttgatgtat tgaaagttga agtaccagta aacatgaagt atgtagaagg ctttgctgaa
     5101 ggtgaagtgg tttatacaaa agaggaagcg gcacaacact ttaaagatca agatgcatca
     5161 actcatttac catacattta cttaagtgct ggtgtttctg cggaattatt ccaagaaact
     5221 ttaaaatttg ctcacgaagc tggtgctaaa ttcaacggtg tactttgtgg ccgtgctaca
     5281 tggtctggtg ctgttcaagt atacattgaa caaggtgaag atgctgctcg tgaatggtta
     5341 cgtacaactg gtttcaaaaa tattgatgac ttaaataaag tattaaaaga tactgcgaca
     5401 tcttggaaac aaagaaaata agtttaaaag gggattaatg ctatgaatag agaagaagtc
     5461 caattattag gttttgaaat tgttgcattt gcaggggatg cacgttctaa gtttttagaa
     5521 gcattaacag cagctcaagc tggagatttt gcaaaagcag atgcattgat tgaagaagga
     5581 aacaattgca ttgctgaagc gcatagagca caaacaagct tgttagctaa agaagcgcaa
     5641 ggtgatgata ttgcatacag tgtgacgatg atgcatggtc aagaccattt aatgacaaca
     5701 attttactga aagatttaat gaagcattta ttagaatttt ataaaagagg gttatgacaa
     5761 tgatgcaaaa attgatcgca caaattgaaa aagggaagcc atttttcgag aaattatcac
     5821 gtaacatata tttaagagcc atccgtgatg gatttatttc tgcaatgcct gtcatcttat
     5881 tctcaagtat ctttctatta attgcatacg tgccaaatat atttggtttt aaatgggata
     5941 aaggcatgga agccatttta atgaaaccat ataactatac gatggggctc gtagcatttt
     6001 tagtagcagg aacgacagcg aaatctttaa cagattcatt taaccgtaag ctagaaagta
     6061 caaatcaaat taactttatt tctacaatgc tagcagcgat gtgtggattc ttgttcttag
     6121 cttctgatcc agccaaagat ggtggattct taagtgcgtt tatgggaact aaaggtttat
     6181 taacagcatt cttatcagca tttgtaactg ttattgttta taacttctgt gtgaagcgca
     6241 atattacaat taaaatgccg aaagaagtac cgccgaatat ttcacaagta tttaaggact
     6301 taattccatt ttcagcggta atcatcattc tttatgcact agatttagtc attcgcaaca
     6361 gctttaaatc aaatgtagcg gaaggtattt taaaattatt cgaaccatta tttacagcag
     6421 cagatggatg gataggtgtc acaattatct ttggtgcctt tgcattattc tggtttgtag
     6481 gaattcatgg tccatcaata gtagaaccag caattgcagc cattacatat gcgaatatcg
     6541 aagcgaactt caagttgctt caagctggtg aacacgcaga taaaattatt acatcaggta
     6601 cacaaatgtt tatcgttaca tttggtggta cgggtgcaac attggtcgtg ccatttatgt
     6661 ttatgtggat gacgaaatct aaacgaaaca aagcgattgg acgtgcatct gttgtaccaa
     6721 cattctttgg tgtaaacgag ccgattttat tcggggcacc actagtattg aatcctgtgt
     6781 tctttattcc atttgtatta gcgccaattg tgaacgtatg gatatttaaa cttttcgttg
     6841 aagtgttagg aatgaacagt tttagtgtga acttaccatg gacaacacca ggtccactcg
     6901 gcatcatcat gggtactgga tttggtttat ggtcattcgt actagcgatt actttaattg
     6961 ttgtagatat tattatttac tacccattcc taaaagttta tgacagtgaa attcttgatg
     7021 aagaagaagg acgtaaagaa agtaattcag atttaaaaga aaaagttgca gcaaactttg
     7081 atacgaaaaa agctgattca attttagcgg caagtggtgt atcagacgat gcagctaaag
     7141 cttcaaatat cacagaacaa acaaatgtct tagtactttg tgccggtggt ggtacaagtg
     7201 gattattagc caacgcactt aataaagcag ctgaagaata tcatgtgcca gtgaaagcgg
     7261 cagctggcgg atatggcgca catatggaca ttatgaaaga gtaccaatta atcattttag
     7321 cgccacaagt tgcgtctaac tatgaagata ttaagcaaga tacagataga ttaggtatta
     7381 aattagcgaa gactcaaggt gctgaatata tcaaattgac acgagatggc caagcagccc
     7441 ttgatttcgt tcaacaacaa tttgaaaatt aagaatagga gtttttcata tgactaaaac
     7501 attacctgaa gattttattt ttggtggagc aacagcagca tatcaagcag aaggtgcgac
     7561 aaatacggat ggtaaaggtc gcgtagcatg ggatacgtat ttagaagaaa actattggta
     7621 cactgcagaa ccagcgagtg atttttataa cagatatcca gtggatttag aattaagtga
     7681 aaaattcggt gttaatggta tccgtatttc aattgcatgg tctcgtatct tcccaaatgg
     7741 atatggcgaa gttaacccta aaggtgttga gtattaccat aagttatttg cggagtgtca
     7801 taaacgtcat gttgaaccat ttgtaacatt gcatcatttt gatacaccag aagtgttaca
     7861 caaagatggc gattttctaa atcgtaaaac gattgactac tttgtagatt atgctgaata
     7921 ttgttttaaa gaattcccag aagtaaaata ttggactaca ttcaatgaaa ttggaccaat
     7981 tggtgatggc caatatttag taggtaaatt ccctccaggt attaaatatg attttgaaaa
     8041 agttttccaa tctcatcata atatgatggt tgctcatgca agagcagtaa aattatttaa
     8101 agatggcggc tatcaaggag aaattggtgt tgtacacgca ttgccaacaa aatatccgtt
     8161 cgatccatca aatcctgaag atgttcgagc agcagaatta gaagatatca tccataataa
     8221 attcatctta gatgcaacat atttaggtaa gtattctcgt gaaacgatgg aaggtgtgca
     8281 acatatctta tctgttaatg gcggtaaatt aaacattaca gatgaagatt atgccatttt
     8341 agatgcagcc aaagatttaa atgacttctt aggtattaat tactacatga gtgattggat
     8401 gagaggttac gatggagaat ctgaaatcac gcataatgcg acaggtgata aaggcggttc
     8461 taaatatcaa cttaaaggtg tcggacaacg tgaatttgat gtcgacgtac ctcgcacaga
     8521 ctgggactgg atgatctatc ctcaaggttt atatgatcag atcatgcgtg ttgttaaaga
     8581 ttatcctaac tatcataaga tttatatcac tgaaaatggt ttaggatata aagatgaatt
     8641 tattgaatct gaaaaaacag ttcatgatga tgcacgtatt gattatgtaa gacaacattt
     8701 aaacgttatt gcagacgcta ttaaagatgg tgcgaatgta aaaggttact ttatttggtc
     8761 attaatggat gtgttctctt ggtcaaatgg ttatgaaaaa cgatatggtt tattctatgt
     8821 cgattttgag actcaagaac gctatcctaa gaaaagtgct tattggtaca aagagttagc
     8881 cgaaacaaaa gaaattaaat aattagagat attaaaaggc ctatcagtcg agtgctggta
     8941 ggtctttatt tataaaaaga gataaattaa acatgcatat acatatttat atattaatca
     9001 atgggtgaca caccattgaa agttaaataa gcaatcaatt aacaaacgct aacggacaac
     9061 tttccaaagt catcttaaaa gttaatgatg tattcaaaat caactaaagg acatttttaa
     9121 tcacatcctt tatacttata taaaagacat gaatgggagt taaaagtgtg aaaaaaattt
     9181 ttgtaattag tacgacattg ttagcggtcg ctattattat aggcactatt attacggttg
     9241 tgtttagtca acgacaggca caaacattta aaatacaaca acagcaattt gttaaaaagc
     9301 caattccgac attgttttta catggatttg gtggtagtgc caactctgaa aaatttatgg
     9361 tgaagcaagc agaaaaaaga ggggtaacta aagatatcat cactgcttac gtttcaaagg
     9421 atggtgcagt gacatttaaa gggaaactaa gtaaagatgc agtgaatcct attgtgaaaa
     9481 ttgaacttga aaacaacaga caaggctatt tggataagaa tgctgcgtgg tttaaaaatg
     9541 tactgactaa gttacaatct gaatacaatt tcgataaatt caactttgtc ggtcactcaa
     9601 tggggaactt aacatttgca caatatatga tgacatatgg taacgacaag tcgctgccac
     9661 agttaaacaa acaagtgaat attgcaggga catttaatgg tgttttaaat atgaatgaag
     9721 atgtcaatga aattacagtt gataaagatg gtaaaccaag tcgaatgaat caaccttatc
     9781 agcaattacg cgtgctaaaa gatatttata aaggtaaagg tatcgaagtg ctaaatattt
     9841 atggagattt gaaagatggt acacattcag acggcagagt atctaatagt tcttcgaaat
     9901 cattaaaata tttactggga aatagtccga aaagttatcg agaatctaaa tatgaaggtg
     9961 agtctgcaca gcatagtcag cttcatgaga atgaaaatgt tgctaatgag ttgatcgact
    10021 tcttatggaa aaaataatgt ctattagtag tcactagaat caaaaagttc tgatatcacc
    10081 tttaaagtag ctataacgat aaaatagtag atacttgtta acgtgatcat cagagtttct
    10141 ttttcaatca gcatggaaga tacgtgccca agtcgtaaca ttttaaattt atgattatag
    10201 caaatataat gtggttgaag ttaaatgaat cagtgcttaa gtcaaaatat aacaactaag
    10261 aatgtatttg catacagaca ttacgaagac acaattaaaa cttaacgctt aagtaacaaa
    10321 taagtaaaag tttcaaataa agaaaggtga ttaatgatgc aaaataaaca aattcttttt
    10381 aataaaatac cagaaggcat gcctcaagag gatacattca aaattgaaga tattgacacg
    10441 cctaaacttg agagcgatgg tgtacttgta caaacgctgt atatttcagt ggatccatat
    10501 atgagagggc gtatgactaa ggcagattcg tatgtacagc cttttgaaat tggtaagccg
    10561 attgtcagtc atattgttgc aaaggtaatt gattcgacac tcgcagatta taaaaaagga
    10621 gatgtcgttg taggtatgtt accttggcga atcattaatc atgtccaagc gaatcaaatt
    10681 actaaagttc caacgacaga tgtaccactt gatttatatt taagtgtctt aggaatgcca
    10741 ggacaaacgg catatcatgg tctgcttgat attggacaac ctaaagccgg tgatactgtt
    10801 gtggtttcag ctgcatctgg tgctgttggt tcagttgtag gtcaaattgc taagattaaa
    10861 ggttgccgtg ttgtaggtat tgcgggtggc gacaaaaaag taaattattt aacagagaca
    10921 ctgggctttg acgctggtat tgattataag aaagaggatt ttgcagaatc gttagcacaa
    10981 gcagtaccag atggtattga tgtttatttt gaaaatgttg gtggtacagt tggcgatgaa
    11041 gtatttaagc atttaaatcg ttttgcacgt gtaccagtat gtggcgcaat ttcgtcatat
    11101 aatcatcctg aagcagatat cggaccacgt attcaaggta cgcttattaa gaagcaagta
    11161 atgatgagag gcttcttagt aagtgaattc gctaatgctt ttaaagaagc cagtgaacag
    11221 ctcgcaacat gggttcaaga aggtaaaatt cagtcacaag taaccattga agatggtttt
    11281 gaaaatgcgc cacgtgcatt taaaaattta tttacgggcg ataactttgg taaacaagtt
    11341 attaaagtta cggaataata aagtatacgt ttttacaaat caactttgtt aattgaccaa
    11401 aatttaataa ctttcttatc taaagcaaat ttgttcagta acatggtaga aataagtcta
    11461 aaatgtaata cgaaagtcgt ctgtaattat ttatgtattc aaatgaaaaa ggagtcatga
    11521 tgctgcgata taagcacaca tggcttctat tttgtataat aagaaataag ttcataaatg
    11581 aggtgaaacg atatgaaaat gataggattt gaaaagccct ttaaattgga agaaggaaat
    11641 ttatttaaag tatacgaaca aagaaagcca acgccagaaa acgatgatat cttagtcaaa
    11701 gtaaatagca ttagtgtaaa tcctgtagat acaaaacaac gacaaatgga agtcactcaa
    11761 gcacctcgtg tacttgggtt tgatgctatt ggtactgttg aagctatcgg accagacgtt
    11821 acattattta gtccgggtga cgttgtattt tatgctggat caccaaatag acaaggatca
    11881 aatgcgacat atcaattggt gtcagaagct atagttgcaa aagcgccaca caacataagt
    11941 gctaatgaag cggttagtct accgctaact ggtattacgg catatgaaac gttttttgat
    12001 acatttaaaa tttcacataa tccttctgaa aatataggta aatccgtgtt aatcattaat
    12061 ggtgctggag gtgtaggaag tattgccact caaattgcta aacggtatgg tttaactgtc
    12121 attacaacag catcacgaca agaaacaaca gagtggtgtg aaaagatggg cgcagatatc
    12181 atattgaatc ataaagagga tttagtgcgt caatttaaag aaaaagaaat tccattagta
    12241 gattatatat tttgcaccta taatacagac ttgtattaca atactatgat tgaactgata
    12301 aaaccattag ggcatattac aactatcgta gcttttaatg aagatcaaga tttaaatgca
    12361 ttaaaattaa aaagtataac atttacacat gaatttatgt ttgcgcgtcc aattcatcga
    12421 acgcctgata tgattaaaca acatgagtat ttagaagata ttacgaaaaa tatagaatca
    12481 ggacattacc aaccaacaac gacacaagtt tttgaaggtt tatcacctga aaacttatat
    12541 caagcacatc aattgttgga aaaacagtca atgattggga aattagtaat taatatatag
    12601 attagtaagt aaagtcgcct tatgcagaaa atactgtata gggcgactta ttttttgtgt
    12661 ttagcgacta ttgattctaa agggaaatat ggcaactttg caggtatcaa ttcaataatg
    12721 cattagaaag gcattactta aatacaataa aatactttgt tgttcaatga taaataattt
    12781 gagtgtattt tatatatcat ttttaaaaga tgaagtatta ttaattgtgt ctatataaaa
    12841 ttacaaatga ttaaaatgtt gcagttgtgg gtatgaactt accattacac agaaagggtt
    12901 tgtatttatg aaaaataaat cagtaagtct agtgttttgg gtatcgttag tgatttgtac
    12961 gatatttgtt gcttttggtg caatatttcc gaaacagtta gaaaaactca cgcaaaacat
    13021 tacttcattt attgcgctac atttttcttg gtattattta ttgctcgttt tagttatttt
    13081 atttgtctgt gtgtatatat tattttcaag atacgcatca attacattag gtgaagaagg
    13141 tgaagaccca gaattttcat tgccatcttg gtttgctatg ttatttagtg ctggtatggg
    13201 tataggactt gtgttctgga caacggcaga accaatcagt cacgctttca aattaacgcc
    13261 tattcataaa gctggcacgc aatctgctat taatgatgca atgcagtttt catttttcca
    13321 ttgggggatt catgcatggg cagtctatgg tattgtcgcg cttgtgtttg catattttag
    13381 ctttcataaa ggatatcctg gcttagtcag cgctacattg acgcctttat taggtgaaaa
    13441 agcaatgcgt ggccctttag gtggtgccat tgatgtattg gcagtcatag ctacagtaac
    13501 aggcgttgct gcaacattag gtttcggtgc attgcaaata aacgaaggtt tacatttttt
    13561 atttaatgta ccgagtaact tcacgatgca agtaatcttg attgtcattg caaccatatt
    13621 atttacttgg tcagcatggt caggtatcga taaaggtatc aaaacgttga gtaatatcaa
    13681 catgttactt gcttttgtag tacttattgg tctttttatt gttgggccaa cgctttacat
    13741 tttaaataca tttacaaatg gtctaggtaa ttatattgca aatttcttta gtatgagttt
    13801 acgcattccg tctggcggtc aaaaattcca atggttacaa aattggacaa tcttttactg
    13861 ggcatggtgg atatcttggg caccatttgt aggtatattt attgcacgtg tgtcaaaagg
    13921 acgtacgatt aaagaattta ttctaggtgt actttttgtc ccagcggtag tttgctttat
    13981 attctttgcg gtattcggcg cgtcagcaat ttacttacaa gataatcata ttgccgatat
    14041 tgctaaagct gcgacagaaa cggcaacttt tgcaacgcta caacattatc cgctaggttt
    14101 tgtattaagc ttaattactt tacttgttat tatgattttc tttgttacat cagcggattc
    14161 ggcaacttat gttttaggta tgttgagttc aagtggtgat attaatccga agtcatttgt
    14221 taaagtgagt tggggtatca ttatggcatt atttgcaata attatgattt acacaggtgg
    14281 tacgcaagcg atacaaaatt tattaatcat cgcagcgttg ccattttccg tggtcattat
    14341 agcgatgata tggtcacttt taaaatcttt aagtgaagaa aaacctagaa atagtaataa
    14401 ggttttaatt aaacatcgtg atccagacgt attagaatat cgttcttcaa aacatattga
    14461 cgaaaattaa ttaacaaaga ggttttattt acaaaaacta gggaatatat tactaaatgg
    14521 tacttcatct tatatattaa aaataaattt aaagttatta atttattatg tttaagaaaa
    14581 aatctgatgg gtacatattt aatatgtttt taaattaact ttaactaagg aggtgtcgtt
    14641 aggggtgaaa cgacttaaaa actttatcct tggcttatta atagtagtaa tcgtggggtt
    14701 cctgttattc atgtatatcc aagatggacg tataactgaa tatcaagact acttccttca
    14761 atttgaatgg ttccagccat tacttatttc acttgctgca ctattgatat taataggtct
    14821 tatattagta tttagtatct tcaaacctac gcatcgaaaa cctggattat acaaagactt
    14881 cgatgatggt catgtttacg tatctcgtaa agctgttgaa aagacggcat tcgatacggt
    14941 tgcaaaatat gatcaagtaa gacaaccgaa tgtagttgcg aaactatata acaaaaaaaa
    15001 taaatcttat atcgacatta aaactgactt tttcgtacca aataatgttc aggtacaatc
    15061 tttaactgaa gcaattcgtg cggatattaa aaagaatgta gaacatttta cagaaatgcc
    15121 agtacgtaag ttagaagtaa atgtgagaga tcagaaaact tctggtccac gagtgttgta
    15181 agggaggtat atcatggcta acaatcataa ccaaaacgga caagactcta cacaacaagt
    15241 gattaatttt ttaaaagtat ttaaatggag aatcgttggt ttcttagcgt ttctattaat
    15301 cgcgatatta ttcttaacgt taggtttttg gaaaacagta cttatcatcg ttttatgttt
    15361 aattggtgta ggtattgggt atatgaaaga ccgtaagcaa gattttatga attttttaaa
    15421 tcgatggagt taattcatca atttagaatt taattaaaaa aatataaaaa ataacaagta
    15481 aaaggagaat ctattatgac tgtagataac aataaagcaa aacaagcata cgacaatcaa
    15541 actggtgtta acgaaaaaga aagagaagag cgtcaaaaac aacaagaaca aaatcaagag
    15601 cctcaattca aaaacaaatt aactttctct gatgaagttg ttgaaaaaat tgctggtatc
    15661 gctgcacgtg aagttaaagg tatcttagac atgaaaggtg gcttaactga tacattcact
    15721 aatgcattct caagtggcaa caacgttaca caaggtgtat ctgttgaagt tggtgaaaaa
    15781 caagctgctg tagacttaaa agtaatttta gaatatggtg aatcagcacc taaaatcttc
    15841 cgtaaagtaa ctgaattagt taaagaacaa gttaaatata ttactggttt agatgtagtt
    15901 gaagttaaca tgcaagttga cgatgtaatg actcaaaaag agtggaaaca aaaacacgaa
    15961 aaaaataatg aaaacaataa ccaagaaaga caaggtttac aataagttat tcggtaaaaa
    16021 agctcggcac accatttaag tgccgagctt ttttgtgatt tttaataatt tgaatatgga
    16081 aaatgtatta ttctctcatt tgtatagatt gtatttaata agttaatgta atccttgagc
    16141 tcacgactaa taaaattcta taaccttaat tattttctcg atacaaagga ttattaactt
    16201 taatataagt ataatgatgc gcttcatctt caagacgcat tgttgtaaca cacttataat
    16261 ctataaatgg cgcgaacatg gtatctttaa tttcatttat gcgattctca tttacttgat
    16321 tagatttgtg tgtcgaaagt acaagttgat caaagatgtt atctagtaca tcacgaacga
    16381 tataccacat atgtctttct aagtttgaat cttttgatgc tttagagatt gtaagaatta
    16441 attcgcctaa atggttttga acggtagaat aaaaggcttt gttaaacaca gacgtttttg
    16501 aatcagtaag aattcttgat ttttcatgga aatgagatgt actgtatccc atttcattta
    16561 gttgtgcttt atcaattctt aaaccttcaa agtctctaat atacatcgtg tccaatagac
    16621 catctttccg aaatattgca atagcatttt gcaagtgagc ctctaatgca atgccatatt
    16681 tagtaactag tgggattacg agaccaagca atgctttact ataagtttca atccacgatt
    16741 tcgccgatga ttcaaaatta gacattgatg cagctgattg ataacgttta atcaatgtca
    16801 cgataggtga ttcattgtta aatgggtagg ttgcaactaa acttgaagga atcattggtg
    16861 tgacttcttg tggaatcatt tggtatatgt tttttctaaa taatgtacct aattgttcac
    16921 ttcgttcagt ttgatagtct gcttcgtctt gctcattata aaaatgaata ccagcaactt
    16981 catcaataat tgttgatgca taggacttaa atatgacatc tttctccaaa atatcattta
    17041 aaatacgtgt cattagtgga ccattgtgcg tcgtttgttc tgataatgta cgaatttcac
    17101 ctgtaatatg aacgtttgtc gacaatttga tgtgtggcga catagctggg tatttaggaa
    17161 ctaatgttct gaaagataaa ccagcataat aatccaacgt atgttttgct tctatgatta
    17221 attctttatc tacttctgct tgataatcag aatgtaatac gtcatctaat tgccatggat
    17281 gtacaatcat aatgtgataa tcattaaagt taaagtttgg tgtaaattca ttttccaatt
    17341 gtttaattaa gtccggaaat agttgatgaa cagttgtatc ataatcttta gacagtgaca
    17401 tagtacgact taatttactg tgaatcagta ctattttcaa cttaataggt tgattgaatt
    17461 ctgaagaata taggaatgtt tgtaatgcat ttaaaccttt acgtaattta gccccgggat
    17521 gtagcggatg accttcaata acggcttgct ctgaacgcaa gtaactatct tcgctatttt
    17581 cgataatatt aaataaaggt gcagaatcat gttgcattga cagtgcttgg tagctaattg
    17641 caaatgtcat attagttgca ctgtttatta aatcttgctg aaattgatca ctagcagcat
    17701 tttttaaatc tggttcttca attaaaatac actcaagaat ttcattagga tggtgtactc
    17761 gtgtaatcat atttgtaatg ttatctttaa tgtagaaagg gccttcaaca tcaattcgat
    17821 caaaggcgtg ttctccagtg ataggagcat ataatgtttg cttagcttgt ggaaactgga
    17881 tttctagtat atgagtcgtt gagatatcta acataatcaa atcacgactc aatattttct
    17941 tactttgagt gcgcgcttta actaagtttt cgcgatgcat tgatgtcact aatctctgag
    18001 tgactttatc tcttccttgt aaaatcatct ctttaaaaat attagcccaa tcgctattat
    18061 gttgttgtaa aaataaatat gtttcttgtt cttctttgtt aaattttaat gtctgttctt
    18121 taaaaattaa gttcaagttc ataattcacc tctatgaaat attttacaaa agcaagatag
    18181 atttgtataa tccatattaa tgataatgat tcttattatc aacagaatgc gggtgtaagt
    18241 tttatgacaa aatatttttt tagcagttct tttctactat ttctaggtaa ttggattgga
    18301 caaatagggc taaattggtt tgtacttacc acttatcata acgcagttta tctgggaatt
    18361 gtcaattttt gcagacttgt accaatatta ttactaagtg tgtgggcagg ggcaattgcc
    18421 gataaatatg ataaagggcg attgctgaga attacaattt catcatcatt tttagtaact
    18481 gcaattttat gtgtgcttac gtatagtttc actgcaattc caattagcgt cattattata
    18541 tatgcgacat taagagggat tttaagtgcg gttgaaacac ctttaagaca agcaatctta
    18601 ccagatttat cagataaaat atctactaca caagctgtat catttcattc attcatcatt
    18661 aatatttgtc gttcaatagg gcctgccatt gctggtgtca tattagcagt ctatcatgcg
    18721 ccaacaacat ttcttgcaca ggcaatttgt tattttatcg cagttttatt atgtttacca
    18781 ttacatttta aagtaactaa aatacctgaa gatgcagcaa gatacatgcc gttaaaagtc
    18841 attatagatt acttcaaatt acatatggaa ggtcgacaaa tatttataac atcattattg
    18901 attatggcga caggtttttc atatacgaca cttttaccag ttttaacaaa caaagtattt
    18961 ccggggaaat ctgaaatatt tggtatcgct atgacgatgt gtgccattgg tggtattatt
    19021 gcaacgatag ttttacctaa agtacttaaa tatattggta tggtaaatat gtattattta
    19081 agttcatttt tatttggtat tgctttgtta ggtgtggtat ttcacaatat tgtcatcatg
    19141 ttcatttgta ttacattgat tgggttattt agtcaatggg cacgtacgac aaatcgcgtt
    19201 tattttcaaa ataatgttaa agattatgaa cgtggtaaag tactgagtat tattatgatg
    19261 gatagaggta tgattccatt gggaagtctg ttaatgagta tatgtgcaga tgtgtttggc
    19321 attgttagaa ctttttcaat aatgggaata agtactatat gcattacaat ggtattctat
    19381 cttataaata gaaaattgaa gttaaagttg gaggaaagta atcatggtat atcttgaatg
    19441 ggcaaaggca gatagaaata ttcaatatcg tgtaattaac gccattatta aagaacgtat
    19501 ttaccccgag caaacattta tttcgcaaaa aggatcttta attgaaattc agtatcatat
    19561 gcatgtgttg actattgaag ttgttagaaa aagtgcatta gaacgctatg agtttacagg
    19621 tgatattact tatttaaata aaggtgaaac gtcgctaatc atgacgttag aagggctact
    19681 agatgtgttg aatcatgact ttgatatccc tatttcagag cgactacgcg aagagttaat
    19741 acacagtcga gatagtttag ttgaaacata taagcaaatg tctcacagac aaacattaat
    19801 aagtcaaagt tttaaatttt caagattacc acaagatatt aacttttttt catggttaca
    19861 acatgtaaaa gatagtgata agacagatga tttaacttat tctgagagtt tggtaccaga
    19921 ggggcatcca acacaccctt taaccaaaac gaaattgccc ttaactatgg aagaagtacg
    19981 agcatatgca cctgagtttg aaaaagaaat ccctttgcaa attatgatga ttgaaaaaga
    20041 ccatgttgtg tgcacagcta tggatggtaa tgatcaattt attattgatg aaataattcc
    20101 cgaatactac aatcagattc gtgtgttttt aaagagttta ggtttgaaaa gtgaagacta
    20161 tagagcgatt ttagtacatc cttggcaata tgatcatacg atagggaaat attttgaagc
    20221 atggattgct aaaaaaatat taattccaac gccgtttaca atactttcaa aagcaacttt
    20281 atcattcagg acgatgtctt taattgataa accatatcat gttaagttgc ccatcgatgc
    20341 acaagcaaca agtgccgtta gaacagtctc aactgtgact actgtagatg gaccaaagtt
    20401 aagttatgct ttacaaaaca tgttgaatca atatccagga tttaaagttg ctatggaacc
    20461 gttcggtgaa tatgcaaatg ttgataaaga tagggcacgt cagcttgctt gtattataag
    20521 acaaaagcct gaaattgatg gaaaaggtgc aacagtagtt agtgcaagtc tagttaataa
    20581 aaatccaata gaccaaaaag ttatcgtgga tagttactta gagtggttaa atcaaggaat
    20641 tactaaagaa agtattacga catttattga acgatacgct caagcattaa tcccgccttt
    20701 aattgctttt attcaaaatt atggaattgc attagaagca cacatgcaaa atacagtagt
    20761 gaacttgggg ccacattttg acattcaatt tttagtgaga gatttaggtg gttctagaat
    20821 tgatttagaa acattacaac atcgtgtatc agatattaaa attacaaatg atagtttaat
    20881 agctgattct atagatgcag tgattgcaaa attccaacat gctgttattc aaaatcaaat
    20941 ggcagaatta atccatcatt ttaatcagta tgattgtgtt gaagaaaccg aattatttaa
    21001 catagtacag caagtagtag cgcatgccat taacccaaca ctaccacatg caaatgagtt
    21061 aaaagatatt ttgtttggac caacaattac tgtcaaagcg ttgttaaata tgagaatgga
    21121 aaataaagta aagcaatatt taaatattga gttagataat ccgataaaaa aagaggtgta
    21181 gtactacatg gcacacgtta acataaatat atcgaagatt aaatataacg ccaaagtact
    21241 tcaaacagtt tttcaaagta aaaatatgca attcacacca gtaattaagt gcatagctgg
    21301 tgaccgtaca attgtagaaa gcttaaaagc gttaggtatc aatcatgttg cagaatccag
    21361 attggataac ataattagta ttgcagatca ggatttaaca tatacgttat tgcgtacacc
    21421 tgccaaaaaa gagatttcag atatgataga aaaagttgat atgagtatcc aaacagaatt
    21481 atctaccata catcatataa atgaagtagc agaagtttta ggaaaaaaac ataagatttt
    21541 attaatggta gattggaaag atggccgtga gggtgttctg acttatgatg ttttagatta
    21601 tattaaagag attattcact tgaaaaatat ccattttgtt ggtttagcat tcaattttat
    21661 gtgttttaaa tctgatgcgc caagtgatga tgatatcttt atgattaatc gattcgtctc
    21721 agctgttgaa agagaaatag gatatcgatt gaaaattatt tcaggtggta attcgagcat
    21781 gttaccacaa cttttatata atgatttggg taaaataaat gaattgcgaa taggtgaaac
    21841 gttgtttaga ggtgttgata caacgacaaa tcaagcgata gctatgttat atcaagatgc
    21901 gattacattg gaggctgaga ttttagaaat taaacctcga gttaatacac aaacgcatga
    21961 atcattcata caagcaattg tagatattgg ttatttagat acaaaagtcg ataatatttc
    22021 acctatggat cagcatatca atattttagg cgcttcgagt gatcatttaa tgcttgattt
    22081 aaatggtcaa ggacactatc aagttggaga tcatattagt ttttcactta attatgaagc
    22141 actttctcat tcgatgtata tgaaaaattt acataaggta tatattgatg attcaaagat
    22201 tgatacgcta cttcaaaatt ttgatgtgaa atctcctgct atggtgaacc aatattaata
    22261 tatttatcaa taagtctaag ttgactagtt atactacata tcgtttacac ggttgtatga
    22321 taattgttca acttagattt tttgtattta atgatttatc aaattaagtg caacagttcg
    22381 tcacataaaa ctgcaatagg taatatcagc tgaattaaag agataacggt catgctaaat
    22441 ggtgtcaatt gtattaattc aaagatgata tagccatgat acattatcag tattttgtct
    22501 aaggaaatgt gctaattgta gtcataatta ttagagggaa taaatatagt catacatttt
    22561 agtaatataa aaagattgaa cgctacttga caatgataat tgttatcaat aaaataataa
    22621 atgaagttat acatattaag gagtggaacg atgagaggtc taaaaacttt tagtatattg
    22681 ggattaatag ttgccttatt tttagttgca gcttgtggta atacggataa ttcaagtaaa
    22741 aaagaatcat caactaaaga tactatttcg gtaaaagatg aaaatggtac agtaaaagta
    22801 cctaaagatg caaaacgtat cgttgtacta gagtactcat ttgcagatgc attagcagca
    22861 ttagacgtta aaccagttgg tattgctgat gatggtaaga aaaaacgtat cattaaacca
    22921 gttagagaaa aaattgggga ttatacttct gtaggtacac gtaaacagcc aaacttagag
    22981 gaaattagta aattaaaacc ggatttaatt atcgctgata gcagtagaca taaaggaatt
    23041 aataaagaat taaacaaaat tgcaccaaca ttatcattaa agagttttga tggtgactac
    23101 aagcaaaata tcgatgcttt caagacaatt gcgaaagctt tagataaaga aaaagaaggc
    23161 gaaaaacgtc ttgctgaaca tgataagtta atcaataagt ataaagatga aattaaattt
    23221 gatagaaatc aaaaagtact tccagcagtt gttgcgaaag ctggtttatt agcacatcca
    23281 aactattcat atgttggaca atttttaaac gaacttggat ttaaaaatgc attaagtgat
    23341 gatgtaacaa aaggtttaag taaatacttg aaaggacctt acttacaatt agatactgaa
    23401 catttagctg acttaaatcc tgaacgcatg attattatga cagataatgc taaaaaagat
    23461 tctgctgaat tcaagaagtt acaagaagat gcaacatgga aaaaattgaa tgcagttaaa
    23521 aataatcgcg ttgatattgt tgaccgtgat gtttgggcaa gatctcgtgg cttaatttct
    23581 tctgaagaaa tggctaaaga acttgttgaa ttatcaaaaa aagaacaaaa gtaaggtgga
    23641 agtaaatggc tataaaagaa ttaagtagcc aatctgccat agatcataaa agaaaaagac
    23701 gcacaacact cacgtatatg gtgagtgtgt gctttctttt tatttgtgta tatttaaata
    23761 tggcaattgg ttcttcaaaa attaatttta gcgatatcgt tcactatgtt actggtcata
    23821 cggatacaaa agcaacgttt ttattacata atgtacgtat gcctagaatg cttgcaggtt
    23881 tatttattgg cggtgcgtta gccgtttctg ggttgttaat gcaagcaatg actcgtaacc
    23941 cactggcatc tccaaagatt tttggtgtta gttctggcgc gtcatttgtc attgtattag
    24001 taacgattat tattccatca ttagaatact atgcacttta tttaggtgtc attggtgcct
    24061 ttattggtgg attaactgtc tacacacttt ctggtgcaac aaaaggcatg acgccaatta
    24121 aattagcatt ggctggtatg gctattcatt tattttttag tagtatgact gaagggatta
    24181 taatcttaaa tgaaaattct aatgaacaag tgatgttttg gcttgttggt tcattatcca
    24241 gtatgaagtg ggatgagatt ttaaccattc taccatggct tattggcgca ttaattgcaa
    24301 cagtctttat tggtcgtcag ttaacgataa tggaacttgg cgatgatatt gctaagggat
    24361 tgggtcaaaa tataaataaa gttagaatca tcattggact attagttatt attcttacag
    24421 gtatgtctgt atcaatagca ggaccaattg gctttgttgg tttaattgtg ccacatatcg
    24481 tcaagcgcta tgtcagcaaa aactacttag ttatgattcc attaacattt attataggtg
    24541 cagatttatt actactatca gatgtattaa gtcgtttaat tacatatcct tatgaatcac
    24601 cagtagggat tgttacatca ttcgtaggtg cactttactt cttatttata acgattaaag
    24661 gggtgaaaag aatatgatga caacgaaaat tatacgtcgt tatagtttaa tcacaatcct
    24721 tttaattgtg agtatattca taagtttatg tgtagggtct gtcatgattc acccgattga
    24781 tgctattaag ggaatcttta cacaagatga ttttatttta aatgagtatc gtatacctag
    24841 aacgttactt ggtattatta tcggtagtag cttggctata tcaggtgcaa ttatacaagc
    24901 tgtaataaga aacccgttag cctcacctga tgttataggt atttctaaag gtgccagttt
    24961 agctgctgtg attattatta tgatctttcc aacagcacca ttgtttgtat tacctatagg
    25021 ttcattctta ggtgcctttg cagtaagttt atttttatca tttttaattt caaaatatga
    25081 tgttaaaggt tcaaagcttg ccttaattgg tttagcaata ggcgcaatat gtacggcgat
    25141 tgtgcagttc ttattaatca gaaatccact ggaagcaaac aaagcgcttg tttggttgac
    25201 aggtagtcta tatggtcatg atatgtcgaa tatttatact atattacctt ggtttttaat
    25261 aacgatacca atcatcattt tattgagtta tcaattagat gtattgaatc taggtgatgc
    25321 tgttgcaacc gcactcggtt taaaagttaa aactattaaa atgctattgc taattttagc
    25381 agtaatatta gcaggatccg caatttcagt tgtcggtggt attagctttt taggattaat
    25441 tgcacctcat attgcgagac aattaatagg aaataaaaca ttacacgtta ttatcatgtc
    25501 ggggttaatt ggtgctatat tgttaacatt tggtgatggt ttagcccgtg gcattcatcc
    25561 gccactggat attcctgtag gggttatcat tgcaattata ggtgcaccat acttcttaat
    25621 attattacga agaatgaaat aactaacata tgattagagt ttaaaaaagg ttaagtacga
    25681 aatcagtaat tgtacttaac cttttttgaa ttcatcttga aaaattacaa gaaatagcgt
    25741 atatttaata actgtaatta ttcattattt tgcatacata aacattttga aagatataat
    25801 gataaggaat atgtttgtta actgaataga attctgtata tacttaaaac gaatacatag
    25861 agaaatggat tgattttatg gatagctttt caaggaaaga gatagtcatt gggagactta
    25921 agtttattac gatgtcttta attggtatct tattatttct agtaccaatt ccagtcgaac
    25981 aagatggaca gaagcaaaca acgttgccag tggcattttt agcaggcgtg cttaaagatg
    26041 tattaggtgg cgtaatgcca tttttaattg tgacgataat tactttgtcg ggtatcataa
    26101 cactgatttg ttcaactatt ttaaaagata aattgaaacc cgatggatta ttgcataatg
    26161 cgtttaatgt aagaataggt tggttaattt tgcgattact tgccgttgtc tttgcgtgga
    26221 tgacattttt acgaattggt tcaaaagtaa tatattcaga tgaaacgggt ggattattgt
    26281 tctcgagttt attgccaact ttagtggcag tattcttatt tgcggcatta tttttgccac
    26341 tattaatgga atacggtttg ttagagatgt tgggtcctat ttttagaccg gttatgagac
    26401 ctttatttac attgccagga cgttcgacgg tagataattt agcatcattt attggagacg
    26461 gaactgttgg ggtcttaatt acaagtagac aatatggtga aggatattat tctcgtcgtg
    26521 aggcaacagt tatttcaact acatttagtg tagtttctat cacctttgca attgtagttg
    26581 ctgaaacagt gcacatgcaa aatcaatttt ttgctttcta tttatcagtg atagtatcct
    26641 gcctcgttgc agcagtcatt atgccaagaa tttggccttt aaataaaatt cctgatgagt
    26701 atgctaagga ggtacccgaa agtgcgcgta ctgaggcatt gccagaagga aagacggcat
    26761 tgagacatgg ctttgatact gcaacagaag taggtattaa agcaccgggc attgttgact
    26821 tctttaagtc aggattgaaa acggttgttg atatgtggtt tgttatttta cctgttgtga
    26881 tgagtattgg tactattgct accattattg cgaactacac gccctttttt gtcatattgg
    26941 gtaaaccttt tgttccattt ttagagttaa tgcaaatacc tgaagcggca caggcttctc
    27001 aaacgattat tatcggtttt gccgatatgt ttttaccttc tatattaatt gaaggtgtac
    27061 aaaatgatat aactcgtttt gttattgggg cacttagtat atcgcagtta atatacttgt
    27121 cagaagtagg tggcgtcata cttggttcca agattcctgt aagcatcggc aaattgttta
    27181 tgattttctt gattagaaca atcattacac taccgattat ttctttgatg gcacacttgt
    27241 tattgtaatt agatatagaa aaggacaact gttaaaattg tggaaacagt tgtccttttg
    27301 tatttgttta tgctgggtgt tgaatcaatt aaaaaaaatc attcatgaag ccctcaccat
    27361 gaatgatttt tttatacaat ttatttagtt aattcattgt aaactttttg atcattttga
    27421 gtataaataa tgtattcgtg gtcttcagta tcgataataa cgcgattcgt tttaccaaat
    27481 gttgagccga ttctagatac atctttgctg tctaatttag gaactgcgtg aatatcttgg
    27541 tcttgtgtaa tatttttaat ttcacttgta ggaattttga tatcagcaac tctccattga
    27601 atacgcactt cattattatc tttttttact gtcattgcca ttattcaaac acatccttta
    27661 aatattttgt aaatacatca taaacgattt tgtaatcgtt ttcaagagtt atgtgatact
    27721 tttttaaatt gtgtcatact aatgaagccc aacccgaaaa tatgttcgtg tttttggcga
    27781 atttatggta aaatgtgagt attaaaggag aacaaatatg acaggtaaaa cacatgcttc
    27841 atgcggcatg ctcgttggag cgctaacaac acaatatttt caaacggata ttttttcgtc
    27901 ggttacggtt atcattctag caactctagc tagcttatta cccgatatat gtcacactca
    27961 aagtaagatt ggcagaaggt ttaaggtaat tagttttttt gtgagattga tctttggaca
    28021 tcgaaccttt acgcattcaa tattatttat cgcaatcata gcatttttat tgcaaattat
    28081 tcagacgcct aattattata tggcagcaat tataattgga ttagtgtcac atgttatact
    28141 agatatgata acaccaaaag gcgtgaaatt attttatccg ttaccgttta atgtgaagac
    28201 accaatccaa tttaaaacag gtggacttgt agacttatct ttagctactg cgttaatggt
    28261 cggaacagtt tatgttttat ttcagccttt catcaatgat ataataagca actggaatac
    28321 taagttttta tagaaaataa attattaaag gttacaaact cagcaggagg cataaaatgc
    28381 tagataaaaa tcaattagct aaatataaac aagatcattt atgtgaatat gaaaaaataa
    28441 tgagtaacaa tgaaaaagaa gcgcttgaag agaaggtagc gtcattagat ttagatttta
    28501 ttgccaaatt atataacgat ttatacatca ataagaaaac aatagatgat gtatctgcag
    28561 tttctgaagt gaaatatgat attaaatcac aaatgagtga tgatgagatt aagcgtttag
    28621 aggaacaagg acttcaagct attaaagaag gacaatttgc agtacttttg atggcaggtg
    28681 gacaaggaac aagacttgga tataaaggac ctaaaggttc attcgaaatt gaaggtgtaa
    28741 gtttatttga acttcaggca aaacaattaa aagaactaca tcgacaaact ggtcatatga
    28801 ttcaatggta cattatgaca agtgatatca atcatgaaga aactttagct tattttgaat
    28861 cacataacta ctttggttat gatcaagaag caatacattt ctttaagcaa gataatattg
    28921 tggcgcttag tgaagcagga caattaattt taaatcaaca gggtcgtatt atggaaacgc
    28981 cgaatggtaa tggtggcgtg tttaaatctt tagacaaggc aggatactta gaagaaatgt
    29041 ctaataatgg cgttaagtat attttcttga ataatatcga taatgtttta gtaaaagtat
    29101 tggatccact atttgcagga tttacggtag aacatgatta cgatattact tctaaaacga
    29161 ttcaaccaaa accaggagaa agtgttgggc gattagttaa tgtcgattgt aaagatacag
    29221 tattggagta ttcagaatta gatccagaag ttgcaaatca atttaacaat gctaatatcg
    29281 gtattcatgc atttaaactt ggttttattt taaatgctgt aaatcgtgaa ttaccttatc
    29341 atttagctat taagaatttg aagcaattag atgagaactt tggtgttatt gaacaaccaa
    29401 ctttaaaatt cgaattgttt tattttgaca tatttactta tggaactagt tttgtcacgt
    29461 tacaagttcc tagagaagaa gaattttcac ctcttaaaaa taaagaaggt aaagatagtg
    29521 ttgcaactgc aacagaagct ttacgtagaa tgggtttaat ttaatgatag gcggtgacta
    29581 tgatgtccaa atcaactaaa aaaagtaatg acacaatcgt agaaacattt aaggatatca
    29641 tccctttatc atttggagaa gaaattggta atgcggcatc tcatggtgtt gcagctttgt
    29701 taacattatt agtgctacct tatgcggctg tacatagtta tattgtaaat ggcacacttg
    29761 catcagttag tatgtcaatt tatgttattt ctatctttat gatgtttatt tcatcaacaa
    29821 tttatcactc tatgcaaaat gaaacgcccc ataaatatat tttaagaatt attgatcata
    29881 gtatgattta cgtagcaatt tcaggtacat atacgcctat tttgttaaca attgtaggtg
    29941 gttggatagg ttggacagta tttattttat tatggggaac gactctttgg ggaattttat
    30001 ataaatccat agctgtcaaa gttaatcaaa agttaagttt aattgtctat ttagtgatgg
    30061 gctgggtagg tattatcttg ttaccaatta tttttattcg cacatcatgg atattcatat
    30121 tattcatttt cttaggcggt gtgtcatata cgattggtgc atggttctat gcacaaaaaa
    30181 acagaccata ttttcatatg atatggcata tttttattgt tatagcatca ctattgcatt
    30241 taattgcaat cttatatttt atgtagtcgt ttagaaaagg tggggggaac tccgcttttt
    30301 ttattactta aatcattcta ttagaggatt gttttgtaat aaaattatcg ttaagctttg
    30361 aaagtcaaaa gtgtttatac atataaaaat caatagtttg taaataaaat cttataaata
    30421 tatagaagga ggtatagcat gcgtttaaaa tcaattgtaa ctgtaatagc tttaatatta
    30481 atcatgttta tggctgcaat cgaatcatca attatttctt tagcgttacc aacaattaag
    30541 caagatttaa atgcagggaa tttaatttca ttaatattta cggcttactt tattgcatta
    30601 gtgattgcga atccaatagt aggtgaatta ttaactcgtt ttaaaattat ttatattgct
    30661 atcgcaggtc ttacactatt cacggttggt agtttaatgt caggtttaag cacacatttt
    30721 agtatgttga ttatatcacg tgtaattcaa gggtttggat ctggtgtcat gatgtccttg
    30781 tcacaaattg tgcctaaatt agcatttgaa attccattac gctataaaat tatgggtatc
    30841 gttggtagcg tttgggggat ttcgagtatc attggaccac ttctaggtgg tggcatatta
    30901 gaatttgcaa catggcattg gttattctac ataaacatac cgattgcgat tattgcaata
    30961 atattggttg tctggacatt tcattttcca gaagaagaaa ctgtggctaa atcaaaattc
    31021 gatactaaag gtatcaccct gttttatatt ttcattggtt taattatgtt tgcattgtta
    31081 aaccaacaac atttatattt aaatatcata ggttttgtct tagcaatatt agttgcactt
    31141 cgattattta atgttgagaa aaaagtttca tccccattct taccagtagc agaatttaat
    31201 cgaatgatta ctttggtctt tattacagat cttcttacag ctgtttgttt aatgggcttt
    31261 aatttatata ttcctgtgta tcttcaagaa caactggggt tatcgccatt gcaaagtgga
    31321 ttagttattt ttccactttc tgtagcgtgg attacattga attttaattt gcaccatatt
    31381 gaagcgaagt tatcaagaaa agttatctat ttgtcgtctt ttacattatt attattaagt
    31441 agtataatca ttgcgtttgg tataaaatta ccaattctta tagcatgtgt cttaatttta
    31501 agtggactaa gctttggata tatttataca aaagatagtg taattgtcca agaggaaaca
    31561 agtccaatac aaatgaagaa aatgatgtcg ttttatggat taacaaaaaa ccttggcgca
    31621 tcaataggtt cgacgattat gggatacctt tatgcattaa aatcaggatt gtttggtgca
    31681 aacttacata atattttagg cgttgtatcc ttgataagtg tagggctcat cgttgtatgg
    31741 ctcatatttt atagagaagc ggcgtcacaa acaaaagaat agaattatga ttaaaattga
    31801 tgtagtgtaa ataaagtaac aaataatcat tttactaagt ttgaagtgta atcaactatc
    31861 ttaaaattaa aagcaatata aattatttat ggtaaggagg acaatagtta tgaattatct
    31921 taaacataag ttttataatt tattaacaac aattattgtt ctctttatct ttgtgctttc
    31981 tggtgcgata ttcttaacgt ttttagggtt cggactatat ggattaagta gaattcttat
    32041 ttattttagg ttaggtgact tcacatataa cagaagtatg tacgacaatt tgctatatta
    32101 tggtagttat attatatttg gctactttat cgtatttgca gttgaacatt tgatggatta
    32161 ttttagaaaa atgttacctg aaaatgctta ttttagaggt acaacttttc atttaatctc
    32221 ttatatggtt gcaacaacgt tattttattt tattattcat ctgaactatg tgtatatcaa
    32281 tattgatttt tgggtcatca tggttattat aggcttttta tatgtttgta agctgcagtt
    32341 ttaccctgaa agtaaaaatc taaataatag aaaatagagt tttgaagttg ggggcaatga
    32401 tgtgttccaa cttcttttta ttcatatagt agaaaatgag ttgttttgct t
//
LOCUS       DAPIDM010000016        31112 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000016 DAPIDM010000000
VERSION     DAPIDM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.016"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(122..889)
                     /locus_tag="RJE42_000651"
     CDS             complement(122..889)
                     /locus_tag="RJE42_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5876103.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAVPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(1251..3062)
                     /locus_tag="RJE42_000652"
     CDS             complement(1251..3062)
                     /locus_tag="RJE42_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV5876104.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKSQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(3737..4438)
                     /gene="isaA"
                     /locus_tag="RJE42_000653"
     CDS             complement(3737..4438)
                     /gene="isaA"
                     /locus_tag="RJE42_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDV5876105.1"
                     /translation="MKKTIMASSLAVALGVTGYAASTGHEAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSDVQSVSYNAQSSNSNVAAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLSAWGF"
     gene            complement(5045..6100)
                     /locus_tag="RJE42_000654"
     CDS             complement(5045..6100)
                     /locus_tag="RJE42_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDV5876106.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVILIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(6227..6355)
                     /locus_tag="RJE42_000655"
     CDS             complement(6227..6355)
                     /locus_tag="RJE42_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876107.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            6510..7079
                     /locus_tag="RJE42_000656"
     CDS             6510..7079
                     /locus_tag="RJE42_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5876108.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            7277..7777
                     /locus_tag="RJE42_000657"
                     /pseudo
     CDS             7277..7777
                     /locus_tag="RJE42_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7997..8227
                     /locus_tag="RJE42_000658"
     CDS             7997..8227
                     /locus_tag="RJE42_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDV5876109.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(8585..8962)
                     /locus_tag="RJE42_000659"
     CDS             complement(8585..8962)
                     /locus_tag="RJE42_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5876110.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIESKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDFDGHHFNFLVC"
     gene            complement(8979..9800)
                     /locus_tag="RJE42_000660"
     CDS             complement(8979..9800)
                     /locus_tag="RJE42_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDV5876111.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSVSLQSAMKGKYGLYSIQPIVKDDVNEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTKLSLSDWLKVQDYNK"
     gene            complement(9819..10118)
                     /locus_tag="RJE42_000661"
     CDS             complement(9819..10118)
                     /locus_tag="RJE42_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDV5876112.1"
                     /translation="MSLNKEQRRITAEELQANFEESTLSVQMIAEKLNVTTEDVEKVF
                     AMKTPLGIFSHQLQRFIHLVWDVRNVINDNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            10249..10806
                     /locus_tag="RJE42_000662"
     CDS             10249..10806
                     /locus_tag="RJE42_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5876113.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITQFKQIKDGYHSNYEVMQVSLDYLLQFKIDHKALL
                     QCIEAGNNKLRFYQSTFYQELFDFYYDLFKSDDDIYTKFKTDMLLQALSTRVFDFQIE
                     HRNISIEAYRNYLLNIYLDEVERND"
     gene            10799..11503
                     /locus_tag="RJE42_000663"
     CDS             10799..11503
                     /locus_tag="RJE42_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV5876114.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSTPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIDA
                     DGPTGTFINGNGEMPW"
     gene            11601..12611
                     /locus_tag="RJE42_000664"
     CDS             11601..12611
                     /locus_tag="RJE42_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV5876115.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPRGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPISYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            12631..13461
                     /locus_tag="RJE42_000665"
     CDS             12631..13461
                     /locus_tag="RJE42_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5876116.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDKPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWNDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSKFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(13608..14498)
                     /locus_tag="RJE42_000666"
     CDS             complement(13608..14498)
                     /locus_tag="RJE42_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV5876117.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLNESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAQFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(14598..15938)
                     /locus_tag="RJE42_000667"
     CDS             complement(14598..15938)
                     /locus_tag="RJE42_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDV5876118.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAVIDQTGLKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMSNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(15932..17038)
                     /locus_tag="RJE42_000668"
     CDS             complement(15932..17038)
                     /locus_tag="RJE42_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDV5876119.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFIATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNDGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFATTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(17374..17670)
                     /locus_tag="RJE42_000669"
     CDS             complement(17374..17670)
                     /locus_tag="RJE42_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDV5876120.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGEVTQQ"
     gene            complement(17658..18026)
                     /locus_tag="RJE42_000670"
     CDS             complement(17658..18026)
                     /locus_tag="RJE42_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876121.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKQEVKALIDESDALNHSIE
                     QLYQHLSERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNIEIEMVTKEENKWKQ"
     gene            complement(18041..18319)
                     /locus_tag="RJE42_000671"
     CDS             complement(18041..18319)
                     /locus_tag="RJE42_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDV5876122.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPEKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRTASDNIQKVGEEFDQTDVMIGNEIGKN"
     gene            complement(18660..19526)
                     /locus_tag="RJE42_000672"
     CDS             complement(18660..19526)
                     /locus_tag="RJE42_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV5876123.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWRTIFVDKRLDHLKEALLNRGLWD
                     ANDIKVYDKVRSQIVAELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTG"
     gene            19744..20808
                     /locus_tag="RJE42_000673"
     CDS             19744..20808
                     /locus_tag="RJE42_000673"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV5876124.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLSSANKVEEGGLSGRPLFERNLRL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            20963..21238
                     /locus_tag="RJE42_000674"
     CDS             20963..21238
                     /locus_tag="RJE42_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876125.1"
                     /translation="MAKETDNKKTVATSDNKDKQQQNQTEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(21424..22146)
                     /locus_tag="RJE42_000675"
     CDS             complement(21424..22146)
                     /locus_tag="RJE42_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDV5876126.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            22500..22949
                     /locus_tag="RJE42_000676"
     CDS             22500..22949
                     /locus_tag="RJE42_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDV5876127.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(23030..23617)
                     /locus_tag="RJE42_000677"
     CDS             complement(23030..23617)
                     /locus_tag="RJE42_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5876128.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IVFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(23595..23703)
                     /locus_tag="RJE42_000678"
                     /pseudo
     CDS             complement(23595..23703)
                     /locus_tag="RJE42_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23760..25442
                     /locus_tag="RJE42_000679"
     CDS             23760..25442
                     /locus_tag="RJE42_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDV5876129.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLFLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(25571..25954)
                     /locus_tag="RJE42_000680"
     CDS             complement(25571..25954)
                     /locus_tag="RJE42_000680"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV5876130.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKHHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(25956..26807)
                     /locus_tag="RJE42_000681"
     CDS             complement(25956..26807)
                     /locus_tag="RJE42_000681"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV5876131.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVKQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTQYLESHISGCIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(26800..27618)
                     /gene="panB"
                     /locus_tag="RJE42_000682"
     CDS             complement(26800..27618)
                     /gene="panB"
                     /locus_tag="RJE42_000682"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV5876132.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            27691..28551
                     /locus_tag="RJE42_000683"
     CDS             27691..28551
                     /locus_tag="RJE42_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV5876133.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     EPAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQESQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(28637..29341)
                     /gene="budA"
                     /locus_tag="RJE42_000684"
     CDS             complement(28637..29341)
                     /gene="budA"
                     /locus_tag="RJE42_000684"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDV5876134.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAKIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(29618..30577)
                     /locus_tag="RJE42_000685"
     CDS             complement(29618..30577)
                     /locus_tag="RJE42_000685"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDV5876135.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
BASE COUNT        10176 a         5746 c         4761 g        10429 t
ORIGIN      
        1 cattttatta cttagttccc taaagattag tgaataaacc gctctcattt atatttatat
       61 aaaacaaaaa cgccaatact ctcatgttag agaatattga cgtcatttga tttacatttg
      121 attaatgaat gaaattatat gaacctgctt ggtttgctga aattgtacga gatgtaacca
      181 caccagcacc atgtccatag ttcatttctg aaacacgaac agaaccgtta ctgttaacgc
      241 cttcaacgta agcaacatga ccgtaatagc cttgtgttgt ttgcatgata gcaccagctt
      301 ttggtgtatt gttcactgta tagccagatg atgcagctgc gctagcccaa ttactagcat
      361 tgccccatgt tgaaccaatt ttcccaccaa cacgatcaaa tacataataa gtacattgtc
      421 ctgaagtata taagttactt cctgatgcat aaccattaga aattgaacga ccatttgaag
      481 atggcactgc agttgtagtc acatgaacat tattgcttgt tgtgctataa cttgcacctg
      541 aaccaccagt gtaatagtta tttgttgctg tttggctatt gtttgtatag ttattgtatt
      601 gatatgaatg attatagtag ttattgtaag atgcattgtt ataactataa gtattgtagt
      661 tgttgtagta gtatgtgttg ttttgcgtta attgacttgg attccaattt cctgtccaag
      721 tgtaatgata attaccttgt gcatcaattg tatacgtata gctgtatgat tgagcgtcat
      781 tagaattata tccattgtta ttttgttctg cggcttgtgc atcatgtcct gcaaatgcga
      841 tagtggcaag tcctgctgta gcgattgtag ctgtaacgat ttttttcata attaaatatc
      901 ctcccaataa tcaaaacact ctatacatat agtcattagc gactcaatta ttttaaattt
      961 tatatttttt atttttttag tatgcaaata attataactt ttatagtttt gcgacaaaac
     1021 aaactataac agagattagg tgtcttgcgg gctttgttat cataatgtaa tctgttccta
     1081 acaaagcatc attgaatgta atataccaat tagaatgttt acaacacttt ttctgcaccg
     1141 ttaacatggg atttgaaaat aaaaaaagca ctaaactttt aatgaaatac atcctttttg
     1201 ataataaata tcagaagtaa tgtaatattt caaaagttta gtgctttaag ttatttctta
     1261 tttgtagcat gtgtttccat cgtttttaca atcaaatcag tcaatgcttt actacctgca
     1321 tattctaaat gtatgccgtc atatgcaaag tactccggat gacctgcaga agctttatac
     1381 caatcgacta gatgtacatt agagcgcttt tcagctgcct cataaattaa tttatttatt
     1441 ctaccttcat aatctctagg tactctaata gaaactaaat atatgtctgc ttttccaaaa
     1501 ctatccaata gttcatttaa ttgatctttc gtaaatgcgc catttgtacc aagctccact
     1561 acaacttttt gacctttttt agcatagtct ttatattgcg atttcacaat tggagtagca
     1621 tcaacgagtt gccgtccaac tttaccatca atttgtgcat ttggtatttt cttagtaaag
     1681 acattaccaa tatccaccat gaccgagtca ccaattaata gtggtgacga ctttttaata
     1741 tcctctttgc tattcgctgt ctgcttatcc tcttgcttat cttttttaac gacttttttc
     1801 ttcgttgttt taaattccgt cttcttctct ttcttctcgg cttcatgttg tttgccaagt
     1861 gcatcaaact gtccactgag cacaacgata gacggaacca ataataggat aactaacaca
     1921 gttctagcaa attgactctt ctttttaggt aaaaatgcaa aagctttaaa tcctttttta
     1981 cgtataggtg tttcaataaa gcgatacgaa atttcagcca ttaacgctgt taacaaaatt
     2041 tctataatat aaacgtatac cggtatttgt ccttgtacgt aataactgtt cacaaaaaca
     2101 atgataggat aatgccataa atataagcta tatgatcgtt tacctataat tagcaaaggt
     2161 ttcatactta aaaatttagc aaataaacta gaaggatgta ccgcaatcgc aataatgaat
     2221 aaagttgcaa atgatataat gtaaaatcct ccattatata tccattgatc ttggtctcca
     2281 actataaaga acaaagtcat tagcaccgca aaaccagata tccctataat atctaatgat
     2341 acgacaatct ttttagaaat atcttttttc aaagcaaacg gaggccaaat aaatgctaat
     2401 atacaaccaa gcaataaagt ttgcagtcgt gtatctgtcc caaaatacac acgtgaatta
     2461 tctccagtga tgaaatgaat cactatcata agtcctaaag aaatcaacga cacaataaat
     2521 agcgtttgaa taatatttct cggtttaaat ctatgtaata agaacgtgat aaccaatggg
     2581 aaaagcaagt aaaattgttc ttcaatggct aaagaccata aatgtttgag tggttcaata
     2641 gcaaattggt taaaataatc tacattctgt gaaatgtacc accagtttga aacatagaat
     2701 atagctgcaa tagcatctcg tttcatttgt ataatcaatt ccggtttaaa tatcagtgtg
     2761 aacgtaagca cgacacagat taaaaataac actgccggaa tgagtcgttt cagtcgtcgc
     2821 ttccaaaact ctagcaaatc gattttttgc gtccgataat actcacttat caacaaactt
     2881 gttattaaat aacctgaaat aacgaagaat gtatctactc ctaaaaagcc cccacttaac
     2941 cattgtgcat tcaagtgata aataatgatt cctataactg cgaatgccct caatccatct
     3001 aatccgggta agtatcgcgg ggaatacatt ttttctaaac gtttaaagtc ttttgtatcc
     3061 atgttaataa acgccccatt tatttttctc tattttgtag catatcacaa tatttttgaa
     3121 aataaaatat tgcactgatt ttcattaatt gatttaaccc ttaattaaga tagttttaaa
     3181 ttttttatta agtagaaaac aattattaca gttaatttca ttactgcaaa ccacatataa
     3241 atttgtcgat tttactacat aacatagatt atcatagatt cttgaatttt taacaaaata
     3301 actattattt tcattatatt tttacaaaaa aaggttcgtt ttatatttta tgcatcttac
     3361 tgtaacagaa tcattaagat atgctattcg aatttacttt ttcaaagctg aataaactga
     3421 caaattaacg cgtactgtta ttaaaatata tacacggtga tatctttcca actcattttc
     3481 aaatttaacc atatgaaatg acaaatactc actccatatt taaaaagaat catggtgtat
     3541 tgtttatttc gccttattct aatttaatag tgacattaat tcttctatca tgacattaaa
     3601 atcgtcagga ataataaaat aacatcctaa cctctagaat cagactttaa tttctaataa
     3661 aaaaactctc cccaatttct atgggaagag ctatatattt aatgtttaaa cattactttt
     3721 attaattatg aaggaattag aatccccaag cgcttaaacc ttgtgctttg tatgctttaa
     3781 cagctgcgtt gatttgttgg tcaacagtgt ttgttggacc ccaacctggc atagtttgga
     3841 ataaacctga agcacctgat gggttgtaag catttacttg accatttgat tcacgagcga
     3901 tgattgcagc ccatgtagaa gctgaaacac cagtacgttg agccatgatt tgagctgctg
     3961 atgaaccagt agcacctgca gtattaccat tgcttaatct cactgaactt gaagtagttg
     4021 aagtgctgta gttatggtaa gttggagctg aaacagctgc aacgtttgag ttacttgatt
     4081 gtgcattgta gcttactgat tgtacatctg aaccttggtt gtatgaagta gtgtagtctg
     4141 cacctgcaac gtttgagaaa ccagcagttt gaccattagc tgcttcatag ctccatgacc
     4201 atgtagtacc atttgaagta aagttatatt ggaaaccatc ttttacaaag tggatgtcat
     4261 atgcaccatc tttgattgga gctgcattta attgatcttg gtgattatgc gctaagtcaa
     4321 ctaagtgtgc ttgatcaacg tttacttcag cagcgtgtgc ttcatgtcct gtgcttgctg
     4381 cgtaacctgt tacacctaat gccactgcta atgatgatgc cataattgtc tttttcatag
     4441 taaaaaatcc tccagtaata attgtaagtt tatgttttta gtaattatat tttgaatttg
     4501 aatgtcgtag tgcaagttta aattgtcttt tatttctttc aacggtactc actatatcat
     4561 aaaaaaccag ctagtaaatt acactttctt tacaaaacat tacaatatca agttttattt
     4621 gtaatgttga aatatggctg ttttatactg taatgtgaaa tatgttccct ttagaatcca
     4681 atcaaccctt gaaatagtct ttaacacata agatttttac tatgtttagc tcaactatta
     4741 caactttcgt aatattacag attgtatttt tgttacatag ctgtaatata tctgacatgt
     4801 aacttctctt attttcgtac attaaacgaa ttaaaaagca atcaacacat atatttctac
     4861 aaatgtgttg attgctatta ttgttatata ttcaaagtta ctatgcacac atcttttgtg
     4921 aattatctta tcttttataa gcacaaataa actgcaactc aattatattg ctaaacttta
     4981 ttctcgcaat tcacaataac attaaataat ttttggtctc atattttcaa aaaacatact
     5041 gtccttatcc catgaattta aaaatatcat tagtatataa acgaaacact ttacgataaa
     5101 tgatatctgc aagccaagct gttacaaatg gcacaacaaa gaacgctact acaattagta
     5161 tgacactcaa ccaagcagaa tcaacctcca taaatttaaa tgcattaatc ggtcctacca
     5221 ttcctataaa accaaatcca gctgactctt tcgttccatg aatacctact aatgctgata
     5281 ccaaacctga tacaatggct gttgtcaata ttggtaacat aagaattgga tatttcacca
     5341 tattaggtat catcatttta acgcctccaa agaagacgga taacggcacc cctaaacgat
     5401 tcactttact tgtaccaatt atcaatactg cttcagtcgc ggagatacca attgacgctg
     5461 atccagctgc taaacctgta atacctatcg caaaggcaat ggccacagtt gatagtggag
     5521 aaataataat aagactaaat accattgaaa tcaaaatact catgacaatc ggttgtaatt
     5581 ctgtaaaacc attaaccata ttaccgatgg ctgttgtaat cattttcgta tacggcaata
     5641 ttaaaacacc aattgcacct gaaataccgc caacaactgt tgggaataca atcaatgcca
     5701 tactacctac gcgatgttga ataagtaaaa tgaacaacac tgcaatcgct gctgtaatca
     5761 ttgtattaat taaatcacca atacccgtaa tcatccaagc accatttttg aactgcgctg
     5821 caccgcttcc tacatatgcc gcacttgcca caacagcaat tgctaatggc gataggtcaa
     5881 atttcatggc aaccaatgca ccaatcaaag caggtactgt aaattgaatt gcaacgacaa
     5941 cgcctaataa cgttttaaaa atcggatgat aatccataaa gtatttaaaa atttctccaa
     6001 gtatcgcatt aggaactaaa cccgcaacaa tacctatggc gacacctgat aaaactctaa
     6061 atataaaatc tttgggtgta attgttttaa ttgatgtcat aatatcatcc ttccatttat
     6121 gtatatacat ctttatgcaa ataataaaga gccttaagtt ataagctgcc actagcttaa
     6181 attctaagat gtgcatgccg atgttgttat atttaggcta gcagtatcat ctataactca
     6241 agactatgaa aaatagtata tcacaaaatt ctgaattttt agataaagaa attggcaatt
     6301 tttcaaacat attgttacaa tacactttta ttttatcttc atttttaaaa tccattaata
     6361 taatagaaga aagacattca aatgcttacc aaaaaggtac attatttgtt aggagcgtat
     6421 cagcacttac atatcatcaa tacaattgac aacataatag aagatactga taataagtgt
     6481 taaaacaaca gatgttaggt agtgaacaaa tgatggaaag taaatccata gatccaagaa
     6541 tcgttagaac caaacaattg cttgtagatg cttttcttaa aatttctaga gaaaagaaat
     6601 tatctcaaat tacagttaaa gatatcactg atattgctac gctaaatcgt gcaacatttt
     6661 acgctcattt cactgataaa gaagacctcc tagactacac attgtctgta accattttaa
     6721 aagacttgaa tgataacttg agcatttcta atgtcattaa tgaaaaggta ctgcgtaata
     6781 ttttcatttc aattgcgagt tatatcaaag atgctgcaaa gtcttgcgaa ttaaatagtg
     6841 aagcattttg caacaaagca catcaacgta ttaataatga attagaagat atttttgcga
     6901 ttatgttaga aaacagctac ccagagcatc aacgagatat cattgtaaat agtgcgagtt
     6961 ttttagcagc tggtatatca ggcttagcat tacattggtt taacacgagc caagagacag
     7021 ccgatgtgtt tatcgatcgc aacctcccat ttttaataca tcatatagca catttttaat
     7081 aaaacttggt atttagtcat gcatcttgaa atcactatgt gacttaggtt catacttgta
     7141 cacacaataa aatttaacgt attacgattg attagccgtg tctaggacat aaatcaacgt
     7201 cctatactct acaatgccat attggcagtc gttaactgaa tgaaaataag cttgtcatta
     7261 aaacatagag attttagtga caagcatttt tgtttttgcg tacttaaaca acacttcagg
     7321 caatatgttg tttaggcaac aaatgatatg accgtgttta ttggcaaacg tacgacatag
     7381 tagtatagta tgtctaaaca acacatgtcg catagttgat atacgttgtt taaatactaa
     7441 gataggaggg attgacgtga gcgagacaga tgaacctcag gggtttgaac acacgcataa
     7501 catattaaat attaatcaga atagtcagag tctaatgaca ttcattataa gtaaattaaa
     7561 gtcgaagttg aagcaacaca taataattgc tcgtggtaat aagcgaatcg actatcgact
     7621 gtcgtataac ttttacacta cgtattatga taatgtagaa atcaagcaaa tcgactgtga
     7681 atatacctat gcaatggcca ttgcaatttt aataagacac acgatgtcat tcgacaatgc
     7741 tcatttcttt gctcagttac gtcatcctgt cttataaaac aacattgcag acatgtatat
     7801 caaacggcac ttcaataaca tcactttgcc gtcgtactac tattaaaatc gtgtctcaaa
     7861 ccccttattt taattccaaa aatctgctgg tcaaaagacc gagaaactaa aaacattact
     7921 gaatgtgttg ataaattacc tgatagaaat aaactcaaaa tatatcaaca cttgattcta
     7981 aggaggatat gacaatatga aaattttaga tagaattaat gaacttgcaa ataaagaaaa
     8041 agtacaacca cttactgtag ctgaaaaaca agaacaacat gcattgcgtc aagactactt
     8101 aagcatgatc cgaggacaag tattaacaac attttccaca ataaaagtgg ttgatccaat
     8161 cggtcaggat gtcacaccag ataaagttta tgatcttcgc caacaatacg gttatattca
     8221 aaattaatat ttgctcacga ggtattgcac ttaaggtgcc aactgacctc ataaacaaag
     8281 cccatacgga ttgaagacac taatgtgtcg aggctgggac ataaatcaat gttctatgct
     8341 ctacgaagtt atattggcag tagttgactg aacgaaaatg cgcttgtaac aagctttttt
     8401 caattctagt caaccttgcc ggcggggccc caacaaagag aaattggatt cccaatttct
     8461 acagacaatg caagttgggg tgggacgacg aaataaattt ttcgaaaata tcatttctgt
     8521 cccactccca cattacgctt catctctgta tgggcttttt atttattctt ttgagaattt
     8581 cattttagca gactaaaaaa ttaaaatggt gtccatcaaa atctttgaac attgcaccat
     8641 aaaacccttg gcttactgtt ggttcttgaa cgactgtgcc acccgcttct ttcactttat
     8701 tcaccaactg gtcaacttct tcatttgtgt tcacacctag agaaatcatc acttcatttg
     8761 gttcagtgtt aaggctttgc tgacttacat tttgaaaatg cttgctttct attaaaatta
     8821 cggttgtttg acctatttga atgccgacca ttttatctaa catttgtggg tttctattta
     8881 ttttaaatcc taacgcttta taaaactgtg cgctcttttc taaatcttga acatgcaaat
     8941 taaaccacat tgattgaatc atgattgcac cccattcatt acttattata gtcttggact
     9001 ttaagccaat cacttaatga taattttgtt ggatttattt cagccattaa ttcaaagtct
     9061 acttcataac ctttttcttc caaccattgc ttttctgcaa caccactaac aaattctcct
     9121 tctataacag tcgatttacc tgtcacttca ctaaaaattg ttgctgcttc acttaatgta
     9181 acttcatcgg aaccaatctc tattgattga tgcgtaaagc tttgtggatg tgcaaaaata
     9241 tacgatgcaa ttttagctat atcaatagaa gaaatcattg tgaattttat attcggatta
     9301 ataaattctg gtaatgtaat acgttcatct tcgactttag caatgcgtaa aaaattatcc
     9361 ataaagaatg atggtttgat aactgttgca tttatattag attccattaa tctattttct
     9421 atttttgcca gtacttcaaa gtgtgggcca gttcgatttc gattaacccc tcccgcagta
     9481 ctatacacaa tatgttgaat attttcttgc tcagctattt caattatctt catgccttgt
     9541 cttaattctt cgttaacatc atctttaacg attggctgaa tactgtataa accatacttc
     9601 cctttcatcg ctgattgcaa actaacacta tcactcagat caccttcaac gattgataaa
     9661 tgcggatgtc ctatgtctga aagtttacga ttattcttat ttctagttaa tgcacttaca
     9721 taccatccat cctctaacaa ctgttttaca actgcattac cttgcttccc tgttgcgcct
     9781 attactaaaa tatctttcat tttagatcca ctcccctttt atcttaaaaa ccaataatcc
     9841 tctttttcac cttttaaata cgtatatggt tctggtgttt gtccattttc cttaatattg
     9901 tcatttatta catttctgac atcccatact aaatgaataa atcgttgtaa ttgatgacta
     9961 aaaatgccta gtggcgtttt catagcaaat actttttcca catcttctgt agtgacattc
    10021 agtttttctg caatcatttg aaccgataag gtagactctt caaaatttgc ttgcaactct
    10081 tcagctgtaa tacgtctttg ctctttattt aaagacatac gcaacactct tttcttttat
    10141 gatatttaat agttattgca aattcattgc taactttctt aacacgtttt ataataaacg
    10201 gagagttatc cgtttgtcaa ttttagagaa ctataagtag gtggaatcat gcgaaaagat
    10261 gcaaaagaga ataggcaacg aattgaagaa atagcgcaca aactttttga tgaagaaggc
    10321 gttgaaaaca ttagtatgaa ccgtattgct aaagaactag gtatcggtat ggggacacta
    10381 tatcgacatt ttaaagataa aagcgattta tgctactacg tcatacaaag agaccttgat
    10441 atttttatca ctcagtttaa acaaattaaa gatggctatc attctaatta cgaggttatg
    10501 caagtgtcgt tagattatct tttacaattt aaaatcgatc ataaggcact actgcaatgt
    10561 attgaagcag gcaacaataa attacgtttt taccaaagca cattttatca agaattattt
    10621 gatttttatt atgacttgtt taaaagcgat gacgacattt acacgaagtt caaaacagac
    10681 atgctgttgc aagcattatc aacacgtgta tttgatttcc aaatagaaca tcgcaatatt
    10741 tcaattgaag cgtatagaaa ttatttatta aacatttatt tagatgaggt ggaacgtaat
    10801 gactaaaatt gtgttaatta caggtggtaa taaaggatta ggctatgcaa gtgcggaggc
    10861 acttaaagca ttgggctaca aagtgtatat aggttctcgt aatgacgtaa gaggacaaca
    10921 agcatctcaa aaattaggcg ttcattatgt acagttagat gttacaagtg actactctgt
    10981 caaaaatgct tataacatga ttgctgaaaa agaaggtcgt cttgatatcc tcattaataa
    11041 tgcaggtata tctggtcaat tttcaacacc ttccaaattg acacctcgtg acgttgaaga
    11101 agtatatcaa acgaatgtat ttggtatcgt tcgaatgatg aacacattcg tccctctctt
    11161 agaaaaatct gaacaacctg ttgtcgtcaa cgtatcaagt ggtttaggtt catttggaat
    11221 ggttacaaac cccgaaacag ctgaatctaa agtgaattca ttagcttatt gttcgtctaa
    11281 atcggcagta acaatgctaa cattacaata cgctaaagga ttaccaaaca tgcaaatcaa
    11341 tgctgctgat ccaggtgcta caaacactga tttagttggt gattttagta ataattcaaa
    11401 acatgtttct gaagggatca agccaattat tcaattagca accattgatg cagatggccc
    11461 gacaggtaca tttattaatg ggaatggtga aatgccttgg taatccaaat ttatgttaca
    11521 aatattgaat ttagaggtaa ataaaaaatg cttctgcaat agatgcaata tacatgcaaa
    11581 acaatatgga ggcgaatcaa atgaaaagta ttacgtttga agaacattat gtcattgaag
    11641 atattcaaaa agaaacgatg aatgcgatat cggcagatcc tagaggtgta ccgatgaaag
    11701 taatgttaga aggccttgaa aaaaagacag gttttacaaa tgccgacgaa ttatcacatc
    11761 atgatgaacg tatccaattt atgaataatc aagacgttca aatccaagtc ttatcttatg
    11821 gaaatgggtc tccttcaaat ttagttggtc agaaagccat tgaattatgc caaaaagcaa
    11881 atgatcaatt ggcaaactat attgcacaat atcccaaccg ctttgtaggc tttgcaactt
    11941 tacctatcaa tgagcctgaa gccgcagcgc gagaattcga acgttgcatc aatgatttag
    12001 gattcaaagg tgcgcttatt atgggacgtg cacaagatgg ttttcttgat caagacaaat
    12061 atgacattat tttcaaaaca gctgaaaatt tagacgtacc gatttatcta catcccgcgc
    12121 cagttaacag tgacatttat caatcatact ataaaggaaa ttatcctgaa gtaactgcgg
    12181 caacatttgc ttgttttggt tatggttggc acattgatgt cggtattcat gcaatacatc
    12241 tagtattatc tggcattttt gatcgttatc caaagttaaa tatgattatt ggacattggg
    12301 gtgagtttat ccccttcttc ttagaacgta tggatgaagc tttattcgct gaacatttga
    12361 accaccctat aagctattac tttaaaaata atttttatat cacaccgagt ggcatgttaa
    12421 cgaagccaca atttgattta gtcaagaaag aagtaggtat tgatagaatt ctttatgctg
    12481 ctgattatcc atatattgag cctgaaaaat taggtgtatt tttagatgaa cttggtttaa
    12541 cagatgaaga aaaagagaaa ataagttata caaatggtgc taaattatta ggtttatcat
    12601 ctaacaatta acaaatggag gtcattgaat atggaaactt tagaattaca aggcgctaaa
    12661 ttacgatacc accaagtcgg acaaggaccc gtgctcatct ttattcctgg tgcaaacggt
    12721 accggagaca tttttctgcc tcttgcagaa cagttaaaag accattttac tgttgtagcc
    12781 gttgatcgtc gtgattatgg agaaagcgag ttaactgaac cacttcctga ttccgcttct
    12841 aaccctgaca gtgattatcg tgtcaaacgc gacgcacagg acattgccga acttgcaaag
    12901 tcattaagtg ataaacctgt ctatatatta ggttcaagtt caggttcaat cgttgcgatg
    12961 catgtgttaa aagattaccc tgaagtcgtt aaaaagattg catttcatga accaccaatt
    13021 aatacatttt taccagacag tacttattgg aacgataaaa atgatgacat tgtgcatcaa
    13081 attttaacag aaggcttaga aaaaggcatg aaaacatttg gtgaaacatt aaatatcgca
    13141 ccaattgatg caaaaatgat gtctcaacct gctgatactg aagaaggacg aatagaacag
    13201 tacaaacgta cgatgttctg gtcaaaattt gaaattagac aatatacgca ttcagatatt
    13261 actttggatg atttcacaaa atacagcgat aaaatcaccc tactaaatgg gacagattct
    13321 agaggctcct tcccacaaga tgttaacttt tatataaaca aagaaactgg catacctatt
    13381 gtagatattc caggtggtca cttaggttat attcaaaaac ctgaaggctt tgctgatgta
    13441 ttattaaata tgtggggtta attgatcgtt ctctaataaa acagcttgga cagttttcaa
    13501 attatatttg atggctgacc aagcttattt ttatttacgc ttaaacgttc aaatcaactc
    13561 gttattatac atgaaggcat gttaaccatt ctcttccact gaaaacacta ctgttccaaa
    13621 cattgatagt cttcttttga aatacccttt ccgataagca ccaaatattc tggcactttt
    13681 tttgaaaacg ctacaggtgt taattcatat tgtccttgtg taaattgaat gagatacgta
    13741 tgtgcggtgt cttcaaatgt cataaaccct ttcaacctat aaatatgaga gggaaggcat
    13801 gctaaacgtt ttataaactg tgctttagta catttgactg gtgattgcac aaattgatga
    13861 ttaatcattt gatgtaacga cttatgttta ttgtcagaag ttgctgtcat ttgtctaact
    13921 gatattggca aagtgacgct gccatgcata ccaacttgta tatcggcctc tgggttaata
    13981 acttctaaat ctttcaatag tttgctcgtc gtttcaacat ctgctgaatc tattttatta
    14041 acaaataaga cagaacaata tgctaattgc tcataaaata agccttggat gtctttaggg
    14101 aatgatttaa tgtgtttata catgcttgcg tctattacac caatcatatg tgtaattgtt
    14161 gtaaacggag ctaaaatagg cgttaaacaa gcatctaaga cagagaccgg ttctgcaatc
    14221 ccactacatt caataaatac aatgtctggt tgctctttta aatataattg atgtaactgt
    14281 tctgatacat ccgctttcat tgcacaacag atacacccct ctgtcagttc acttaaaggt
    14341 atgtcttcag atactaaggc accatcaaca ctcattttgc caaattcatt catgataact
    14401 gccggtttca gcgactcatt taatgattca gcgagcaaat gatttaagac agtcgtttta
    14461 ccgccaccta aaaacccacc tataataact attttcatct gcgactctcc tcttatactt
    14521 catatgaata gcatattcca agatagcttt cctattgact gacatgactc tatattcagt
    14581 tatagttcat ttctttttta aataatcagc attactattt taacaatgat gcctacacca
    14641 ataactaaag acaatgatgt caccatttgc tttccaatta attttagtgc tgactgacca
    14701 cctaaatgtt tcaaaagcat cgtcattgtg accgagcacg ctgtaaatgt tgaactaaaa
    14761 aacacaagta gtagtaactg tgttgctgtc attccttgta ataaggcgcc ttgatgcaaa
    14821 ttaaacaaga gcatgccgtc ttttcgaatc attgaaaata aaatccctgg tgacaattct
    14881 gacgagatgc ctaataacga taatataggt gtaaatattt gtgataaaac attcaaaatt
    14941 ggcgttagtg ataaaatact aacaataaga cagattgtga taaaaatagg tagcgcttga
    15001 acgataaaca tttgtatatt ttgccacatt tgtagcaaca tttgacgtat atttgacata
    15061 tgtaattgcc tatcataagg tagcggaaca ctaagttgtt gatcattctt tttcaaccat
    15121 atcctattat gtaagatgcc acctaaaagt actaatatta agtacggcat aaatagccat
    15181 gactttccag ctacactaaa aatagataat gtcgcaccta tttgataact acaagaacta
    15241 ccaaagctta ttaaactcat acactgtgtc ttcgtgcagg tatggcattg gtgtgctgct
    15301 tgtgaaatag ctgctgcatt acatccaaat ccttctaata gtggcacgat atcattacct
    15361 tgtaatccta tccgtaacat tgaaggttca attgcccata tcatccatga tttaagtcct
    15421 gtttgatcaa ttacagcagt tgataagcta atcaatatta cgaccggcaa tgcccatacg
    15481 agcgaatatg ttccaagtga tagcacacca taatcaccaa ataatatatg ctgtaatata
    15541 tcgtgattga gcaatgttgt ttgagttaaa tattctatcc atgctgatac atactgattt
    15601 tgaaaccaat ctgaaaattg ataagcgata ccgattggta atgcaaacat taaaaatatc
    15661 aacaaccagg ctaaaaatgt cgcacttatt ttaggtctta aatgtcgtcg ctcatgttga
    15721 aatagtgtca attgttctat cacatgttcg ctcgctatag gcgaagaaac tacactttct
    15781 gccaatgaaa tatctaattt ctgttttatg attaataatc ttaatttcgc tttttctgtt
    15841 gcggtaagtt tatccgcatg actaataacc aaataatgag gttcattagg tatgggcgta
    15901 taatgccccg ttctcatatc atatttcact tctaacatgt gcatgacact tcacaacatg
    15961 aataatcatc tagatacatt tgattttttt ggtaattttc aatgacctct tgtttagctg
    16021 gtaagccttc ccgctgtgtt aaaagatgtg caagtactgc aaatcgcgct ctgaatttat
    16081 agatatagca taatttggca ttatcatttt caactgtcgc accaatcata aaaatattcg
    16141 gataacgtgt cgattcatca tgtgttgtta atttaatatc ctgatttgtt gtcgcaaata
    16201 attgttgaac gattggattt tttgttgcat caaagccagt tgctagtatt ggttcatgag
    16261 gcgtaagcac actttgtccg ctatcaaaac tgatatgata ctgtccatcg ttaaaatcaa
    16321 tatctttaac tgtataatgc acattcattt caatgcgagc tccttgctta atgacattac
    16381 ctagtcgctg acgtgtataa ggtgacaatc taacactagg atcagcatcc ggatcattta
    16441 aaccggttgt gctagtataa agtgcgatgt cagagccatt ttttgcaagt tgatatgcag
    16501 catcaaagcc actttcatta cctccgataa ccacatattg ccccttatta aagttatcaa
    16561 agtcttcaat ctcactataa tgaataccat atttaaatgg ctttttaggg aaattataat
    16621 cacctgttgc gataaagata taatccgcgt gatatgtctc tgttgtcgtt gcaatcgtat
    16681 aatatgcatc atctacagat atatttgtga caactgtatt ttcaaagata ttcagctcgt
    16741 aatggttggc aaccacttgt aaatattcag catatgtttc tccggaaata tgttcttcat
    16801 taaatgtaaa tgctggtgaa gtatccatgg aaattgcatt catatcaggc atgccaaatc
    16861 cattagacgt aaatgatggc gtaatcgtac gggtcgattt cggccaatgt ttaaatgaat
    16921 gtcctactgt tcctttttct aaaataatga catctgttat accgaaatct tttaaggtaa
    16981 tggccatacc tatacctgca gcaccggcac cgataatagc cactttatga tgttgcatat
    17041 taacactccc tcaatgatct atttcaagtt attgtttttg attataaatc gtaataatta
    17101 cgatttataa agtatagacc tatccttgag aaatttcaat attaaaagaa aaacttccgt
    17161 tattgtagag aattcatcta catttcggaa gttggtatgt gtctttttta ttaaatttaa
    17221 agatgctacg gttagttcaa atgattgcga tagacattca atggttgtca tccagcctca
    17281 gtacttggag ataacatttt ggcggtgaga cagaaatcat attttcacaa aatttatttc
    17341 gtcgtcccac cccaataaag aaaaagtatt tctttattgt tgtgtaactt ctccacgttg
    17401 tatttcacta tccaaatttt gtttcatatt atcaaacaac tcttgaaatc taacctcatc
    17461 ttgatcagaa taacctttaa aaataactga atctatattt tcctcgttaa aataataaat
    17521 ttcatcttcc acaacaccaa tcggatattt acatgcagaa tagtcaaaca tatacttttg
    17581 attttcaatt tctacaattg gtcctctatt aataatcatt aacttttgcg aaccttcttt
    17641 taaataaata atgcttccta ttgtttccat ttattttcct cctttgtcac catctcgatt
    17701 tcgatattcc ttcgcatttc taaatcaatc atctcttctt ccgctttacg aatgccatgt
    17761 tgaatttgtt gttgctgctc gtataacgct tccgttgaac gtcgtttcgc aaaatgaaat
    17821 tcatcgcgga actgttccat acgactagcc atattgctac tataataacg ctcacttaaa
    17881 tgttgatata attgttctat cgaatgatta agcgcatcac tttcatctat caatgcttta
    17941 acctcttgct tttcatcata taagtcatcc atttgcttat gtttcgctgt aatcatttta
    18001 ttgattccat caagtttatt cgacatcatt tcaccacgtt ttaattttta ccaatttcat
    18061 taccaatcat tacgtcagtt tggtcaaatt cttcgccaac tttttgtata ttatcgctcg
    18121 cagttcgtat tgcttcagca acagattgat tcttactaat caaatcatct atcacactat
    18181 gagcattatt atttccactt actgttgtac tttcatcttt ttccggtttc gacacattat
    18241 tacatgtttc aacagcgctt ttaatagatg atgatgcact attggtctta aaaatatcac
    18301 tttgaactgt actcatcatt tacacctctt cccatttaaa ttattttaaa attattagaa
    18361 actgtatcaa tatatgatat tcaaacattc aattatactt aaatattgtt ttgtgattat
    18421 attcaaaatt attgtatcaa taaatatgta tcattcctag aattttattg aacaaaaagt
    18481 tagaaaataa cttttattgc aactcaacct tgttttgcat tatagaaatt aacaatagag
    18541 attaaaattt tattttccaa caataaaaaa gcacaaatat ttttattgag gcagctgtat
    18601 ctcttctctt cataaaaata tctgtgcata ttatttatta tcttaatcta acgtaataac
    18661 taacctgttg tatcttgtaa aatcttatcc atagagtgcg caacactatc acggtacatc
    18721 tccccaaatt tcaataaatg caccatcaat aaatataaac gataaaattc aagtctatag
    18781 gatgcacctt ttgcgagtgg ataatgttta ttatacgcat cataaaattc gctagtaaaa
    18841 ccaccaaata ccgttgtaat accgatatca aattctctgt caccatataa tggtgctgga
    18901 tcaaataacg ccgggcgtcc atcttttaaa aacatataat taccgcccca taaatcacca
    18961 tgtaataaag acggtttact ttgatgcttt tctaattccg ccacaatttg acttcgcact
    19021 ttgtcatata ctttgatatc gttggcatcc catagccctc tatttagcaa cgcttctttc
    19081 aaatgatcca agcgtttatc gacaaaaatg gtacgccaat cgtcttgcca atgattatca
    19141 aatgaaatat cgccaccttc ataaggtaat gagaagccaa atttgccttc ttcttgttgc
    19201 tgactgtgta attgagctac gagttgccct aattgacgtt gactccctga agctccttct
    19261 tctaaatacg tcatcactaa atacgcatca ccgttaactt cgccacttgc aatcactcta
    19321 ggtgccgtga tacctgcacg ctcaaattca tttaaacctg caatttctgc agcataaaat
    19381 gattctttac gcccacgttg aacaagtaag aaaaatgtat ccgtgtctgt ttcgactcga
    19441 tatgcttcgt ttacatcacc accactcact ggtgaaatct ctttaatatc ttttaaaggt
    19501 aaatgctcta accattgttc attcatacaa acaccttcca atctacttac tttatactat
    19561 aacattgcct aattaggatg atttaatctt aaaaacgtta ttcaaacttg cgtcaatttt
    19621 caaaactttt tagttttaac taggaaatta tgtagggaaa tgctagtcta ttaaaagaat
    19681 atatttcaca tatattaagt tgaggatagt aagcctcaaa tataatgaaa taggagcata
    19741 ctcatgtaca aattaattaa acctttctta ttcaaaatcg aacccgaaaa agcacacgga
    19801 ctaactatcg atgcattaaa aacgttacaa aagtttccgg ttttattccc agtcgtcgat
    19861 aaactattta cttataagaa tccaacgtta tcacaaacga tacaaggtaa tacgtacgac
    19921 aatccaattg gtttagctgc tggtttcgac aaatcttgcg aagtgccaaa agcattggaa
    19981 caccttggat tcggtgcttt agaattaggt ggtatcaccc ctaaacctca accgggtaac
    20041 cctcaaccac gcatgtttag attattagaa gatgatgcct tgataaatcg aatgggcttc
    20101 aataatattg gtatgaacaa agcactaagt catttgcgta aaaatgctta tcaagtacct
    20161 gttggtatca atgttggtgt gaataaaatg acaccttatg aagcgcgtta tcaagattat
    20221 ataaaggtta ttgatacgtt taaacacgac gtttcatttt tcacagtcaa catcagttct
    20281 ccaaatactg aaaatcttca aaacttccat gataaagatg aattttcaat gttatgccaa
    20341 gctttaacaa catttaaaaa acaacatgat gtaacagtgc caatttattt aaagctgacg
    20401 tctgatatgg acttcgatgg cttaaaagca ctattaccgg cgattactga aacatttgac
    20461 ggtatcatct tagcaaacac aacgcgacaa cgagatggtt tatcttctgc taataaagtc
    20521 gaagaaggcg gtttgagtgg tcgtccatta tttgaacgta atttaagatt aattaagtat
    20581 gcttatcagc aaacaaatgg tgaattttta attataggta caggcggcgt attcagtact
    20641 gaagatgcaa tcaaaatgat gcgtcacggt gcgtcactta ttcaaattta ttcatcactt
    20701 gttattgaag ggccaggttt aactaagaaa atgaacaaag gcatcgcacg ttacttaaaa
    20761 gatcatcatt ttgacaatgt tagtgacatt attgggctag atgcttaacc caacgtagtc
    20821 aaatgatgct ttaaaacttg ctgaatcgtc ttattaaaat tatgatataa ctcaagaaga
    20881 tatatttatc accatttttt aaaataatgg cttaaatatc atatatcatt aaatatagcg
    20941 ttttataacg gaggcgtttg ttatggctaa agaaacagat aataaaaaaa cagtagcgac
    21001 ttctgacaac aaagacaaac agcaacaaaa tcaaactgaa gaagatgaac gtcaaggttt
    21061 aaatggttat cgtaaaacag atcttgattt agaaattgag caagagctac gtgaaatgat
    21121 gaaaacaggt gaaaatgaaa cgaaaaacga ctacaaaaag tttaaagttt tttctcttat
    21181 ttcaacactt gtcattgtca ttttagcaat tatacgattt gttcataaaa tgatgtaatt
    21241 agagtgagac attgttttat gtctcaggat ccagttattc attatagcta caatatttac
    21301 gattatataa ataacccgag attttagtat gattcatttc actaaagtct cgggtttcta
    21361 tttgataatt tttaataggg catggcttga atatgcgcct gccttttatc tcatttccaa
    21421 tgattaatct ggatattgtt ctaaaaatgc tttcgcttct ttattaactg ttttaaaatc
    21481 aataccttgt tgcatcgctg caaagacaca tccacaataa cactgtctaa agatattata
    21541 gtcattacac atttctatgg aacgctcata acctttactt ttcttaaaat cacttggcaa
    21601 atagttcaca tcatatattt tttggacatc cataccaagt tcattgatta attgtgcgtt
    21661 ctttttaggt gataacgtga ttgcactgcc aaaataatca tagccatgtt ctacagctgc
    21721 tttcgctaca atatctaaac gcatttcaaa acaagcggta caacgtaagc cgccttcttt
    21781 ttcatcagct aattctttat ccttcaccat tttcacaaac ttatgcggtt cataaggtgc
    21841 ttcaatatac ttcacattcg caccagtctt gcgattaaaa tcttccacaa attgttcttg
    21901 cactttagca cgtcgtaaat attcattttt cggatgaata tttgaattcg cgaaataaat
    21961 tgcaatgtct gcatattgtg ttaaaaactc taatgtatat gtactacaag gtgcacaaca
    22021 actatgtaat aaaattttag gtctgattgc ttctctttcc cactgcccga ttaatttctt
    22081 caataccttg tcataattaa ttttttgatt tttcatttta ctgataatag gttcagcatt
    22141 aatcatgatt taacctccca catttaatca ttaacttcta ttatatatga ttcatattaa
    22201 atgtcagtca aaaaagttag aaattcattt taatgcattt atatttcgaa aatcccttat
    22261 gtatctaaaa gcatttttta aacttgaatt ttaaaacact aaacaacaca tacgtctctg
    22321 tgtcattttc attttttgta tatcatatat atgtttactt catttaaatc aatttcatct
    22381 tacaatttat cgtgtatttt acaaaagatt gacttcaatt catcgtaaaa gttatacttt
    22441 tgccattttt taatgtaata tggtgttagt aataaaaata atacattgag gtgttttaca
    22501 tgacagcatt attcccttat atcgcttttg aaaattcaaa agaagccctt gcatattacg
    22561 aagaagtatt tggtgcaact gacgttaaac gtttagaagt tggcgaagaa caagcgtcac
    22621 attttggtat gactaaggaa gaagcacaag aagcaactat gcatgctgag tttgaagtgc
    22681 ttggcgtaaa agtgctatgt tctgattctt ttggtcgcgc tgacaaaatt aataatggca
    22741 tatcattatt aattgattat gatgttaaca ataaggaaga tgctgataaa gttgaagcat
    22801 tctatgagca aattaaagat cattcttcaa ttgaaataga attaccgttt gctgaccaat
    22861 tctggggtgg caaaatgggc atctttaccg ataaatacgg tgttcgttgg atgttacatg
    22921 gtcaagacta tactgcaatc caacaataat gttattgata aagatatcat ttaataagct
    22981 tatgttattt agcaaggcaa gccactcttg attcatggtt tgccttattt tattgtatat
    23041 ttttaattat cgttgctggc ccttgattga caatcgtatt aaaatgcctt attaagtcaa
    23101 ctttgtctat acggtttgga tcttctaccc aatgtcttat caaagacaat cccgcacctg
    23161 aaacataact catgaaatag gaaaatggta taccattaat ttgatcattt tcatttttat
    23221 aacgttgcaa atgacacata attaaatcat aaagtttttc gtggatattt gaatctttac
    23281 ccatacgaaa catcaattga taaaatacga tgtctttttc tatcatttct attaaaacgg
    23341 tcataatttg atgtatgtta tccgtggata acttaactgc tccatttaac ttctcatcat
    23401 gaatgaagtc tcttatttcc tccaactgct ggtcctctaa tttttcaagc aaatcatatt
    23461 tatcataata atgcgtataa aatgtactac ggttcacatc agctaaatct gcaatttgtt
    23521 gtacagtaat ctcttctaat tggtgttgat gtaaaagttc aataaatgca tttctcattg
    23581 caacttgtga ttttctaata cgtcgatcta tagtcattta tatcaagtcc tccccaatga
    23641 ttataaacaa ttataaacgt tatgttcatt atcctacaaa cctccaacat tgatgattgg
    23701 cacacaatgt ttacctgttt aatataggtg atacaaacaa acagaaaaag gtgataacaa
    23761 tgaaccaaca tttactagga aatccaaaat taactgtaac tcatgtcaat gaagtgaaag
    23821 ccggtattaa ccacatcgtt gtcgacagtg ttcaatatgg aaatcaagaa atgattatgg
    23881 aaaaagatgt cactgtggaa atgcgcgatg gcgaaaaatt atatattaat attttcagac
    23941 caaataaaga tggcaaattc cctgtagtta tgtctgcaga tacttacggt aaagataata
    24001 agcctaaaat caccaatatg ggtgcccttt ggccaacatt aggcgccatt ccgacatcta
    24061 gttttacacc tgaagaatca ccagacccag gattttgggt gccaaatgat tatgttgtag
    24121 ttaaagttgc attacgcggt agtgacaaat ccaaaggcgt cttatctcca tggtcaaaaa
    24181 gagaagcgga agattattac gaagtgattg aatgggcagc aaaacagcca tggagtaatg
    24241 gaaatatcgg aacaaatggt gtttcttatc ttgcggtgac tcaatggtgg gtcgcatcat
    24301 taaatccacc acatttaaaa gcaatgattc cttgggaagg attaaatgat atgtatagag
    24361 aagtagcctt tcacgggggt attcctgata ctggctttta tcgtttctgg atacaaggta
    24421 tttttgcgag atggacagat aatccaaata tcgaagacat gattcaagca caacaagaac
    24481 accctttgtt cgatgatttt tggaaacagc gtcaagtgcc attatcacaa attaaaacac
    24541 ctctactaac atgtgctagt tggtctacac aaggtttgca caaccgtggc tcttttgaag
    24601 gatttaaaca agctgcatct gaagaaaaat ggctatatgt gcatggacgt aaagagtggg
    24661 aaagttacta cgctagagaa aatctcgaac gccaaaaatc attctttgat ttttacctta
    24721 aagaagaaaa taacgattgg aaagatacgc ctcatgtcat atatgaagtt agagatcaat
    24781 tttataaagg cgaattcaaa tcagcgtcag cgttcccttt acctaacaca gaatatacac
    24841 cattgttttt gaatgctgaa aatcacacat tgaatcatgc aaagattagt agcgcgcatg
    24901 tcgcacaata tgactctgaa gataaacgac aagatgtaag ttttaaatat acgtttgaca
    24961 aagatactga gttagttgga aacatgaact taaaactatg ggtaagcact aaagattcag
    25021 acgatatgga tttatttgca ggtattaaaa agttagatcg tcgtggtaat gaagttaact
    25081 tccctgattt taatcatatt gaaaatggtc aagtagctac cggttggtta cgcgtatcac
    25141 atcgtgaatt agatcaagaa aaatccacaa ttgcgcaacc ttggcataaa catgaaacag
    25201 aattaaagtt gtcacaagat gagattgtac ctgttgaaat cgaattgtta ccttcaggca
    25261 cgctatttaa acaaggcgaa acattggaag ttgttgtaaa aggtagtgaa attgtaattg
    25321 gtaatagtac ccctggcatg aaaacgcgtt atgaacatga agaaaccgta aataaaggca
    25381 tgcacatgat ttatactggt ggtaaatatg attcacaatt aatcattcct atcgttaatt
    25441 gatatacagc aattacggtc gattttgatt aaaagtgaca tagttatagg actgtataac
    25501 aagagaaggc cacacgcttg gaatcttaaa ccaaggtgtg gcccttttta ttattgatgg
    25561 ctatttaatt ttataacact atcgtatttt ctttttcatg aatcatttca ataatgacat
    25621 tgtcttcatt cattactgct actttaggtg catggtgttt aatttcctct tcattcaact
    25681 gtgcataagt catgattatg actacatcgc ctacttcaac aagtcttgat gctgcaccgt
    25741 ttaaacaaat tttaccacta cctctttcac cagctattac gtatgtttca aaacgtgcac
    25801 cattattatt attcacgatg gctacttttt catttggcaa gatgtctacc gcttccaata
    25861 tatctgaatc aatcgtaatg ctacctacat aatttaaatt tgactcagtc actcttgctc
    25921 tatgaatttt agcattcatc attgttctta tcactttatt cagctccaat tattatatta
    25981 tctattaaac gcgcttttga aaatttgaca gctaacgaga taaatatgcg tccagttatt
    26041 tcgtgttgtt ctactaattg aggataacta taaacagcaa cttcttcaat gcatccactt
    26101 atatgtgatt caagatattg agtaaccctg tctataatta ctttactttg acgttcaccg
    26161 tcttgataca acgcttgtgc taatagcaaa cttttactta aatgtaccgc ttcttgtcgt
    26221 tcttgctccg ttaaataaac atttcttgaa cttttcgcca aaccatctgc ttctcgaacg
    26281 atatcaatac caataatttc aacggcatga ttgaagtctt ttaccatttg cttgacaata
    26341 gccaattgct gggcatcttt tttaccaaaa taagcataat ccggcataac aatattaaat
    26401 agcttattaa ctactgttac caccccatca aaatgccctg gacgcttcgc tccttctaac
    26461 acatcagcta atggacctac tttgacatca atacctaatt cacctggata catatcttct
    26521 actgcaggat gaaaaacaat gtccgctcct acttctgata ctaattctaa atctttatca
    26581 atttgtctcg gataagcatc gaaatcttcg tttggaccaa attgtaatgg attaacaaat
    26641 acactcacaa ttgtaatatc atttgtacta actgattcgc gtaccatcgt taaatgtcca
    26701 tcatgtaagg cacccattgt tgggataaaa ccaatcgttg tgcctgagcg tttggctgct
    26761 ttaacaatgt gttgcatctc ttttaccgta gtaatcagct tagtcattgt tattaacctc
    26821 attcataatt ttctttttat acgtatattc ttctgatgga aatgcaccag atttaacttc
    26881 ttgatcgtat tgttttaaac catctacacc aacactaaaa tcagcaaatt gcttcacaaa
    26941 tttcgcttta tgttcaacac cataatttaa catatcgtga taaaccaata cttgaccatc
    27001 tgtacctttt cctgcaccaa taccaatgac tggaattgtt aaatgcttgc taatttcttc
    27061 tgctaaatca tttggaattg cttcaagtac taacgcaact gcaccagctt gttctacatt
    27121 tttcgcatct aaaataagtt gctcagctgc ttctttcgtt gcaccttgta atttatatcc
    27181 cataacgcca acactttgag gtgttaatcc taaatgtgca acaacaggaa taccaattgc
    27241 cgttgctttt tcaataaatg gtgtaatatg cgctccttct gctttaattg catttgcatt
    27301 cgtctcctga taaagcttta gagcatgatt taagtcttgt gtcatagaga tgcctactgc
    27361 accaattggc atatcaacaa ctacaaatgt atttggtgcg cctcttctta ctgcacgacc
    27421 gtgatgaatc atatctgcta acgtcacttg tacggtactt tcataaccta atacagtcat
    27481 accaagtgaa tccccaacaa gaatcatatc aatacccgct gcttccactt gtttagcact
    27541 tggaaaatca taagctgtta ccatagaaat tttagtttgc ttttgtttca tatctattaa
    27601 ttgacttact gttttcaatg ttattcaacc tctttttgca gtattattag aaatagtata
    27661 atgaatcttt tatagaaagg aaagaagaag atgttatcag ttgcgattat cggcccaggc
    27721 gctgtcggta caactattgc ctatgaatta caacaatcat tgcctcatac aacgcttatc
    27781 gggagacacg ccaaaacaat aacatattat actgtaccac atgaacctgc acaagatatt
    27841 gttgtgaaag gttatgaaga tgtcacaaac acatttgatg ttattattat tgcagtcaaa
    27901 acacatcaac ttgatgcagt tattcctcat ttaactcatc ttgcacatga ggacacgctc
    27961 atcattttag cccaaaatgg ttatggtcaa ctcgaacata ttccatttaa aaacgtatgc
    28021 caagcagttg tctatataag tggtcaaaag aaaggcgatg ttgttacgca cttcagagat
    28081 tatcaattac gtatacaaga taatgcatta actcgacaat tcagagattt ggtccaagaa
    28141 agtcaaatcg acattgtatt agaagcaaat attcaacaag ctatttggta taaattgtta
    28201 gttaacttgg gcattaattc tatcacagca ctcggtagac agacagttgc aatcatgcat
    28261 aatcctgaaa tacgtacact gtgtagacag ctattactag atggttgtcg tgttgcacag
    28321 gcagaggggt taaacttttc agaacaaact gtcgatacta ttatgactat atatcaaggt
    28381 tatcccgatg aaatgggaac gagcatgtat tacgacattg tgcatcagca acctcttgaa
    28441 gtcgaagcga ttcaaggctt tatttatcga cgtgcacgtg aacacaatct tgacacccca
    28501 tatcttgata ctatttatag ctttttacgc gcgtatcaac aaaatatgta agcacatata
    28561 taatataaaa agaccttaac gatgcattta gaaccacctg ttcctaatat cgctaaggtc
    28621 ttttaattat attagactat tcagcttctc taatttcttc tgccacatct ttatagtcta
    28681 ttttcgcttt aacaaacgtc tcgttattta ccgggaaatg ttgttggaat gtttcaaagt
    28741 tttgtatctc aacgacaacg tcatccactt caaagtcaag aacatgtcca ccataagctc
    28801 tttcatcatc cgcaaaatgt atatgaaaac cagcagaccc tacgccatga aataattctg
    28861 gtgtaaaaaa tccaacaatg gcaccacgaa tatcttgtct tttttcctca ggttgtctgc
    28921 gtgctgaatc aatcaaacgt gtatatggcg gttgctgagc aggcatcatt cgtacatgca
    28981 tatgtttaaa tgtgccataa attttaacag ccgaaaataa attctcactt aacatttcat
    29041 ttttaatttt tgcgaataca tcatcttgtg ataattgttg caatggaaat gtcttactcg
    29101 ctttaaaatt agtaatcgat gcatacggta ctttctcatc accttttaat tctataaatt
    29161 ctttatgctc gttagcatga tatgccttcc cgtctaaaaa tattacttcg ccatcagacc
    29221 ctgttaacgt tgcaatccct aaatttccat gttctaacaa ttcattaatt gtagctgttc
    29281 cttctagtaa gccagccatt aacgtaccta atgtaccatg ttgatacaag acattcgtca
    29341 ttccaaaacc atctccctat agttattcct cgttgtcatg ctctacttcg tgcttaatgc
    29401 agaactactt tcctttatac tcaatgacac ctcgacattt ctattcatgt cactaaattt
    29461 tcacgagtca taatacttta ttcaattatt attttacgca aaagtattgc ctctatcaat
    29521 gcttgctaaa aaatggacct atcagtgcca catgttactg caacactaat agatcctctt
    29581 accattaata tatttctaat taatacttaa aataggctta gtcttctaat aaatatttaa
    29641 ttgaatcaaa tgtatcttct aatgttttaa cagatttatc gaacaatgct tgttcttctg
    29701 cacttaatgg catttcataa attttaactg cgccatgttg gttaactaat gttggtacac
    29761 ctaggtaaac gcctttgtga ccaccatatt gaccatctaa ttgtatagaa acatttaaga
    29821 cattattttc attatttaaa atggcttttg aaatgcgcat caatgctaat gcaataccat
    29881 agtatgttga ccctttagct tggataattt cataagcagc gtcacgtgta tttacataaa
    29941 tttcttccgc tttagcttcg ctaccagttt gttcttttaa tgtgtcatat actgaaatac
    30001 ctgctacatt tgcttgtgac caaactgcaa gttcagtatc accatgctca ccaataatac
    30061 tagcgtcaac acttgaaggt gcaacaccaa gttcttggct aattaaatat tgtaaacgtg
    30121 cactgtccaa tacagtacct gaaccgataa cacgctctgc tggcaatcca gtatattctt
    30181 ttacaaatct tgttaaaatg tctacagggt ttgccgcgat taagaaatat ccatcaaagc
    30241 cgctatccat tacactctta acaatgctct tcataatctt agtatttttt tcaactaatt
    30301 gtaaacgtgt ttcacctggc ttttgaggtg caccagctgt aataacaact aaatctgcat
    30361 ctttacagtc ttcgtattca cctgctttca catcaactgg tgaaggactg tggactgtac
    30421 catggtttaa atcttgaaca tctgctttta ctttgtcttt tgcaatgtca ataattacaa
    30481 attcatcagc aacaccttgc gtaaccattg caaaggcata gcttgatcct acagatccat
    30541 ctccgattaa tacaaccttt ttaccaaatg ttttcataat tatgtctcct aacctaattg
    30601 aatttatttt acacgtgact acacgtaaaa atatctatac tgaaattaaa gattaacaat
    30661 tttattttat tataatatta tgctaccaaa ttacaaacat aaaactgaga tgtaagcgga
    30721 gtcaaattac actctaacgc ccataaatat tgatatctca agctttagca cgtaatttga
    30781 aatttgtgac aatttttagc attctaaaat tgcgtaatga cgctatttaa aggctttaac
    30841 aaactattga gctttctatt tttttcattt taaaaaggat ggctattcga cttaaatgac
    30901 ttactatata acctcctatt ttccacctta ccctcatcat atttgatgca tctttttata
    30961 gtcaaagaat tacatttcat acaaactagc caatcagaca taaacatgaa attaatttta
    31021 aaaatataat gcgcaaaatt gttagaaaat tagcaaaaaa tagtacttat tcatttaata
    31081 tctgttataa tcagttttat catttaaaaa at
//
LOCUS       DAPIDM010000017        30074 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000017 DAPIDM010000000
VERSION     DAPIDM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.017"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..293)
                     /locus_tag="RJE42_000686"
     CDS             complement(<1..293)
                     /locus_tag="RJE42_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876136.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVGRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIP"
     gene            331..1005
                     /locus_tag="RJE42_000687"
     CDS             331..1005
                     /locus_tag="RJE42_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDV5876137.1"
                     /translation="MIDKKLASPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTITKVLSALVMGMLWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            1042..1776
                     /locus_tag="RJE42_000688"
     CDS             1042..1776
                     /locus_tag="RJE42_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDV5876138.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNAQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKHIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEEHVIRFFNALT"
     gene            2149..3486
                     /locus_tag="RJE42_000689"
     CDS             2149..3486
                     /locus_tag="RJE42_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDV5876139.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQSFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            3703..4608
                     /locus_tag="RJE42_000690"
     CDS             3703..4608
                     /locus_tag="RJE42_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDV5876140.1"
                     /translation="MITYDLIGNTPLVLLEHYSDEKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEMAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSILNGGVAHAHDTEGIGSEKW
                     PIFLERSLVDGIFTIKDQDAFRNVKSLAKHEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKEIFNYEENDYE"
     gene            4601..5743
                     /locus_tag="RJE42_000691"
     CDS             4601..5743
                     /locus_tag="RJE42_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDV5876141.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQEHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            6008..7033
                     /locus_tag="RJE42_000692"
     CDS             6008..7033
                     /locus_tag="RJE42_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDV5876142.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIQQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMLITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDKAINQYFKEKN
                     IQFEEVH"
     gene            7037..7696
                     /locus_tag="RJE42_000693"
     CDS             7037..7696
                     /locus_tag="RJE42_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876143.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVVIVQVIQTLGNILARFIRR
                     H"
     gene            7733..8575
                     /gene="gmpC"
                     /locus_tag="RJE42_000694"
     CDS             7733..8575
                     /gene="gmpC"
                     /locus_tag="RJE42_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDV5876144.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTNISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSDSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            8908..9912
                     /gene="aaa"
                     /locus_tag="RJE42_000695"
     CDS             8908..9912
                     /gene="aaa"
                     /locus_tag="RJE42_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDV5876145.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNSASNSSRPSTNSSGGSYY
                     TVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVTGNASTNSGSTTTTNRG
                     YNTPVFNHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(10099..10368)
                     /locus_tag="RJE42_000696"
     CDS             complement(10099..10368)
                     /locus_tag="RJE42_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876146.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            10518..10913
                     /locus_tag="RJE42_000697"
     CDS             10518..10913
                     /locus_tag="RJE42_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDV5876147.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEEINLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRSLTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            10903..11388
                     /locus_tag="RJE42_000698"
     CDS             10903..11388
                     /locus_tag="RJE42_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876148.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNKGIGKVV
                     MEKLAPFIISTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(11555..12337)
                     /locus_tag="RJE42_000699"
     CDS             complement(11555..12337)
                     /locus_tag="RJE42_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDV5876149.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(12334..13452)
                     /locus_tag="RJE42_000700"
     CDS             complement(12334..13452)
                     /locus_tag="RJE42_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDV5876150.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDTKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITVASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(13567..14451)
                     /locus_tag="RJE42_000701"
     CDS             complement(13567..14451)
                     /locus_tag="RJE42_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5876151.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNIMLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAESVKKQLRKITKSDVYTI
                     SSLPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            14632..19131
                     /gene="gltB"
                     /locus_tag="RJE42_000702"
     CDS             14632..19131
                     /gene="gltB"
                     /locus_tag="RJE42_000702"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV5876152.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERVLG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLNRIQLKRPVLNESHLAAI
                     NQEHFKLTYLSTVYEGDLEDALEALGREAVDAVKQGAQILVLDDSGLVDGNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISSPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEVGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            19149..20612
                     /locus_tag="RJE42_000703"
     CDS             19149..20612
                     /locus_tag="RJE42_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDV5876153.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            20882..20974
                     /locus_tag="RJE42_000704"
     tRNA            20882..20974
                     /locus_tag="RJE42_000704"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20918..20920,aa:Ser,seq:tga)
     gene            21446..22873
                     /gene="treP"
                     /locus_tag="RJE42_000705"
     CDS             21446..22873
                     /gene="treP"
                     /locus_tag="RJE42_000705"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDV5876154.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKKAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGLVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSGISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            22937..24577
                     /gene="treC"
                     /locus_tag="RJE42_000706"
     CDS             22937..24577
                     /gene="treC"
                     /locus_tag="RJE42_000706"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV5876155.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISDYLEINEDFGTMDDFEKLIKGAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTYGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDIPENYHQVNVRQALK
                     DKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLASEGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            24602..25330
                     /gene="treR"
                     /locus_tag="RJE42_000707"
     CDS             24602..25330
                     /gene="treR"
                     /locus_tag="RJE42_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDV5876156.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQDALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSLAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            25392..25661
                     /gene="ffs"
                     /locus_tag="RJE42_000708"
     ncRNA           25392..25661
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RJE42_000708"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            25731..25832
                     /locus_tag="RJE42_000709"
     CDS             25731..25832
                     /locus_tag="RJE42_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876157.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSEIDAQFE"
     gene            25971..26495
                     /locus_tag="RJE42_000710"
     CDS             25971..26495
                     /locus_tag="RJE42_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV5876158.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            26564..28261
                     /gene="dnaX"
                     /locus_tag="RJE42_000711"
     CDS             26564..28261
                     /gene="dnaX"
                     /locus_tag="RJE42_000711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV5876159.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIASVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            28351..28668
                     /locus_tag="RJE42_000712"
     CDS             28351..28668
                     /locus_tag="RJE42_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV5876160.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            28675..29271
                     /gene="recR"
                     /locus_tag="RJE42_000713"
     CDS             28675..29271
                     /gene="recR"
                     /locus_tag="RJE42_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV5876161.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            29471..29555
                     /locus_tag="RJE42_000714"
                     /pseudo
     CDS             29471..29555
                     /locus_tag="RJE42_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10060 a         4286 c         6260 g         9468 t
ORIGIN      
        1 ggaataaaat ataatacaaa tatccagtaa atcaaatttg aaaacgatgg atgtataggg
       61 gtaaagaatc taactaatgt aatgattaac gctatataga caaaaattac tctattgata
      121 cgccctaccc cctctgtata ataaatatag atcgtactaa attgaaaata gcaaaatatt
      181 gttatttcaa ttatacaatg ttttatttgc aatatacata acaatatgaa ctttataatt
      241 ttgatattgt cggtaaagtg aaatttgtgg cacattgata ttatggataa catttgtaaa
      301 atgaaaatga tgatacgagg agattgtaag atgatagata aaaaattagc atcaccgaaa
      361 atgacagtgc ctttattttt aattgcgctg attgtattta taggtatgtt ttacagtgta
      421 gtgacaaatc aagaatggct taaaaatata gatatgggat cattaacatg gtttacagat
      481 tatttcggtg agccacaacg tcagtatgtt aacaatttat ttaattacta tatgacgttt
      541 agtgcggaaa ttggagatgt caaaggtgtc gtgttgattt ccattatcgt cacaatcata
      601 ctgtttatta aacagaggca tttagcggtt tggtttgtga catatttggt ttcaggtgtc
      661 atcatgaaca aattaattaa agatactgtt ttacgtccaa gaccatataa tcatttagcc
      721 gttgatacag gcttttcatt tccgagtgga cattctaacg ccagcacatt attatatttc
      781 gccttaatga tcattattat ttcagttgct gctaagacaa taacaaaagt gttgagtgcg
      841 ctggttatgg gaatgttatg gcttagcata ttattttgtc gcctttattt tcatgcgcat
      901 tacttttcag atgtcattgg cggcacgtca ctagcaatca tttgggtagc gttattctta
      961 atggtatacc catactttat taatcatcga cgacaacgcg tttagcaaac atctttagac
     1021 atagtggagg tatatagaaa aatgagaatt aaaacaccga gtccatctta tttaaaaggc
     1081 acaaatggac atgcgatatt attattacat tcatttacag gtacaaatcg ggatgtgaag
     1141 catcttgcag ctgagttaaa tgcacaagga ttcagttgtt atgcaccgaa ttatccaggt
     1201 catggtttat tgttgaaaga tttcatgaca tataatgtag atgattggtg ggaagaagtt
     1261 gagaaagctt accaattttt agtcaatgaa ggttatgaat ctatcagtgc aactggtgtg
     1321 tctttaggtg gattaatgac attgaaattg gcgcaacact acccgttgaa acatatcgct
     1381 gtcatgtcag caccaaagga aaagagtgac gatggcttaa tagaacattt agtttattat
     1441 agtcaacgca tgtcgaatat tttaaattta gatcagcaag catcgagtgc gcaattagca
     1501 gcaattgatg attatgaagg tgaaattacg aagtttcaac attttattga tgatatcatg
     1561 acaaatttaa atgttattaa aatgccagct aatatattat ttggtggtaa agatgcacca
     1621 tcctatgaaa caagtgcaca ctttatttat gaacatttag gatcagtaga caaagaatta
     1681 aatggtctga aggattcgca tcatttaatg acgcatggag aaggcagaga tattttagaa
     1741 gaacatgtta ttcgcttttt caatgcttta acataattga agattttaaa aattaacact
     1801 tgagtgacga tgttattata attgtcattt gagtgttttt tgtggcgtga tagtataggg
     1861 aacttagaca aagttaattt gtagtgtgat gttgtggaaa tattatgggc attgtacctg
     1921 aagttgttat gtggcgagat gttggcgcac gtatacagcg ttgaaccaga aattttaatg
     1981 tagcgtaatg acaacacatt acaaggtatt tactatatta tgataaactc aggagctacc
     2041 gtattaatga aacaattttg atgcaggtgt attgcatgtt atttacatac atgttagagt
     2101 gttacttaaa cacgtagacg ctttaacgct ttaaaggaga tgtgaatgat gaaaagacaa
     2161 caatcacaat ggaagtcatc aactggattt attttagcta gtgcgggttc tgcaatcggt
     2221 cttggtgcca tgtggaaatt cccatatatg gcagggatct atggcggcgg tgcctttcta
     2281 gctatgttct taatattcac catttttgtt ggattaccat tactcattat ggaattcact
     2341 gttgggaaaa tgggacggac gtatacaaca caaatatata gtaaattaac tggtaaaaaa
     2401 tggctcaata tcattggttg gaatggtaat ttggcagtgt ttattttatt tggcttctat
     2461 agtgttatcg gtggttggat tgtcatttac atcggacaag tattatggca attagttata
     2521 tttcaacgca tcaatcatct ccaagaaatg aattttgaag cggtaatatc aaatccttgg
     2581 ttaaccgttc tagggcaagg tatattcata ttcgctacga tgattattgt catgttaggt
     2641 gttgaaaaag gattagaaaa ggcatcgaaa gttatgatgc cattgctgtt tgtcttttta
     2701 atcatcattg tgattaagtc tttaacatta gatggtgcct tagaaggtgt gaaatttatt
     2761 ttacaaccaa gagtatcaga gattactgct gatggaatct tgtttgcgct aggtcaatca
     2821 ttctttacgt tatcattagg aactacaggt atgattactt atgcgagtta tgcctctaaa
     2881 gacatgacga ttaagtcatc agctatttct atcgtggtta tgaatatctt tgtatctgta
     2941 ttggcaggcc tagctatatt tccggcgtta catagttttg gctatgaacc acaagaaggg
     3001 cctggattat tatttaaagt actaccaatg gtctttagtc aaatgcatct aggcacatta
     3061 ttctatttgg gattcttagt gctgttctta tttgcggctt taacgtcatc tatttcttta
     3121 ttagaattaa atgtttctaa cttcacgaag aatgacaata caaaacgtaa aaaagtcgca
     3181 gtgatcggta gtattttagt atttatcatt agtattccag caaccttatc ttttggtatc
     3241 ttaaaagatg tgagattcgg tgcgggaacg atttttgata atatggattt catcgtttcg
     3301 aatgtattga tgccattagg cgcattaggt actacgcttg tcgtaggaca attattagat
     3361 aaaaaattat tacaacaatc ttttggtaaa gatagattta gattattcag tggttggtat
     3421 tacttaatta agtatgcgat gccggtcgtt attattttag tctttatcgt gcaattattt
     3481 agttaatata tagaagcatc tgacacacaa gtatttgtgt tgctcaggtg tttttttgtt
     3541 tggaattgaa aagtttgtac ttgttctgaa ttaatcgaaa cttagataat tgcaaaataa
     3601 caaaatgtcg tttataattc ttatctgaaa agtaaggatt gaaattgaga catgaagaaa
     3661 ggtttcattc atttgatcgg catgtcagat gagggtgcat ctatgattac ttatgattta
     3721 attggtaata caccattagt actgttagaa cattatagtg atgaaaaagt taaaatttat
     3781 gccaagcttg aacaatggaa tcctggaggc agtgttaaag acaggctcgg gaaatattta
     3841 gtagagatgg caattcaaga agggcgtgtg cgtgcaggtc aaactattgt tgaagcgact
     3901 gctggcaata caggcatagg gttagctatt gcagcgaata gacatcattt gaaatgtaag
     3961 atctttgcgc cgtatggttt ttcagaagaa aagattaata ttatgatagc gcttggtgca
     4021 gatgtttcaa gaacgagtca gtctgaaggt atgcatgggg cacaattagc tgcacgttcc
     4081 tatgctgaaa aatatggtgc cgtttatatg aatcaatttg aatccgaaca taatccggat
     4141 acatattttc atacattggg acccgaattg acttcagcat tacagcaaat tgattatttt
     4201 gtggctggta ttggctctgg cggtacattt acaggtaccg cacgttattt aaagcaacat
     4261 cacgtgcaat gttatgccgt tgagccagaa gggtctattt taaatggtgg cgtagcccat
     4321 gcacacgaca ctgaaggtat cggttctgag aaatggccga tatttttaga gagaagtctt
     4381 gtagatggga tatttacgat taaagatcaa gatgcctttc gaaatgtcaa aagtttggca
     4441 aagcatgaag gattactggt aggcagttct tcaggtgcag cattacaagg tgcattgaat
     4501 ttaaaagcgc aattatctga aggtacgatt gttgtcgtat ttccagatgg tagtgatcga
     4561 tatatgtcta aggaaatatt taattatgag gagaatgatt atgaataaga aaacgaaatt
     4621 aattcacggt ggacacacaa cggacgacta tacaggtgca gttacaacac caatttatca
     4681 aacaagtaca tatttacaag atgatattgg tgatttacgt caaggatatg aatattctcg
     4741 tactgcgaat ccaacaagaa gttctgtaga aagcgttatt gcgacattag aaaatggcaa
     4801 acatggcttt gcatttagtt caggtgttgc agcaatcagt gcagttgtta tgttgttgga
     4861 caaaggagat catattattt taaattcaga tgtatacggc ggtacttatc gcgcattgac
     4921 aaaagtattt acacgatttg gcattgaagt ggattttgta gatacaacgc atacagattc
     4981 aattgtacaa gcgatacgcc caacaacaaa gatgttgttt attgaaacac cttctaatcc
     5041 attattacgt gttactgaca ttaaaaagtc tgctgaaatt gcgaaagaac acggtttgat
     5101 ttcagtcgtt gataacacat ttatgacacc ttattatcag aatccattag atttaggcat
     5161 cgatattgtc ttacattctg caacgaaata tttaggtgga catagtgatg tcgttgctgg
     5221 tttagttgca acatcggatg acaagcttgc agaacgttta gcatttattt caaattcaac
     5281 aggtggcatt ttaggacctc aagatagcta tttacttgta agaggtatta aaacattagg
     5341 tttacgtatg gaacaaatta atcgcagtgt tattgaaatt attaaaatgt tacaagaaca
     5401 tccagctgtg caacaagtgt ttcatccaag tattgaaagt catttaaatc atgatgtcca
     5461 tatggctcaa gcggatggac atacaggtgt gattgcattt gaagtgaaag atacagaaag
     5521 tgccaaacaa ttgattaaag caacatcgta ttacacatta gctgaaagtt taggtgcagt
     5581 ggaaagttta atttcagtac ctgcattgat gacacatgca tccattccag cagatattcg
     5641 agctaaagaa ggtattacag acggacttgt aagaatttct gtaggtattg aagatactga
     5701 agatttagta gatgatttaa aacaagcact agatacgtta taaataatag tagcactggc
     5761 atatattttg agttcaacat ttgttgttct tgaatatgct agtgcttttt tagtgcgatg
     5821 ttaatatttc gtgattgagt tgtggagatt tttatggatt taatttgacg atagtcatag
     5881 tcttgtcaca aggtattatt ttttaatttt ttagaaaatt cggcaaattg tattgacgat
     5941 atatgcaatt ttacatataa tgctcttatt cctagtggat taataagaat ttgtaggagg
     6001 ggcgatgatg attgagtttc gacaagttag taaatcattt cataagaaaa agcaaacaat
     6061 agatgctctg aaggacgtat catttacggt caatcgcaat gatatttttg gtgtgattgg
     6121 atatagtggt gcaggaaaaa gtacgttggt aaggctcgta aatcatcttg aagctgcctc
     6181 gaatggacaa gtgattgtag atggacatga tattacgaat tatagcgata aaatgatgcg
     6241 ggatattaag aaagatatcg gtatgatatt tcagcatttt aatttactaa attcagctac
     6301 ggtatttaaa aatgtagcta tgccgctcat tttaagtaag aaaagcaaaa cagaaattca
     6361 gcaacgcgta acggaaatgc ttgaatttgt aggattgagt gataaaaaag accaatttcc
     6421 tgatgaattg tcgggtggac agaagcaaag agtggctatt gcaagagcgc ttgttactaa
     6481 tccgaaaata ctcctatgcg atgaagcaac aagtgcattg gatccagcaa cgactgcttc
     6541 gatattgacg ttattaaaga atgtcaatca aacctttggc attacaatta tgttgattac
     6601 acatgaaatg cgcgttatta aagacatttg taatcgtgtt gctgtaatgg aaaaggggca
     6661 agtggttgaa acaggaactg ttaaagaggt gtttagtcat cctaaaacga cgattgctca
     6721 aaattttgtg tctacagtta tacagactga gccaagtaca tcattgattc gtcgattgaa
     6781 tgacgaacaa gttggcgatt ttaaagatta taaaatcttc gtcgaggaaa ctcaggtgac
     6841 acaaccgatt ataaatgact tgattcaaat ttgtggcaga gaggttaaaa ttttattttc
     6901 atctatgtca gaaatacaag gtaacaccgt atgttatatg tggcttcgat ttaatatgga
     6961 tcaacaattt gatgacaagg caataaatca atatttcaaa gagaaaaata ttcaatttga
     7021 ggaggtgcat taacatatgt ttggttctga tttagacagt gcacagttat tacaagcatt
     7081 atacgaaacg ctatatatgg tatctattgc tttattttta ggagcagtga ttggtattcc
     7141 attaggtgtc ttattggtaa ttactcgaaa acaaggcata tggcccaata tagtgataca
     7201 tcaagtttta aatcctttaa tcaatatttt aaggtcacta ccatttatta ttttgttaat
     7261 tgcgattgtg ccattcacaa aattagtagt aggtacttcc attggtacca ctgctgccat
     7321 cgtgccttta acagtatatg tagcacctta cattgcaaga cttgttgaaa actcattatt
     7381 ggaagtagac gaggggatta ttgaagcggc gaaagcgatg ggcgcttcac cactacaaat
     7441 cattagatat tttttaattc ctgaagcgtt aggttcatta gtattagcaa ttaccactgc
     7501 gattattgga cttattggta gtacggcgat ggcaggcgct gttggcggtg gtggtatagg
     7561 agacttagct ttagtgtatg gttatcaaag atttgatacg acggtcatta ttattaccgt
     7621 tattgtatta gtcgttattg tccaagtgat tcaaacgcta gggaatatcc tagctagatt
     7681 catacgtaga cattaatgat atatagtgaa gattttgaaa ggaattgata gaatgaaaag
     7741 attgattggg ttagttatcg tagcacttgt attattagca gcgtgtggtg gtaacaatga
     7801 taaaaaagta acgattggtg tcgcgtcaaa tgacactaag gcttgggaga aggttaaaga
     7861 attagctaaa aaagatgata ttgatgtgga gattaagcac ttctccgatt acaatttacc
     7921 gaataaagca ttaaacgatg gtgatattga tatgaatgca ttccaacatt ttgcattttt
     7981 agatcaatat aaaaaggcgc ataaaggaac aaatatttca gcattaagta caacagtttt
     8041 agcaccgttg ggtatttact cagataaaat taaagatgtc aaaaaggtta aagatggtgc
     8101 taaagttgtc attccaaatg atgtatcaaa ccaagcacgt gcacttaaac tattagaagc
     8161 agctggttta ataaaactga aaaaagattt cggtttggca ggcacggtga aagatataac
     8221 gtctaatcca aaacatttaa aaattactgc agtagatgca caacaaactg cacgtgcttt
     8281 atctgatgtc gatattgcag ttattaataa cggtgtagca actaaagctg ggaaagatcc
     8341 aaaaaatgat ccgattttct tggaaaaatc agattcagat gctgtaaagc catatattaa
     8401 tattgttgca gttaacgaca aagacttgga taacaaaaca tatgctaaaa tcgtagaatt
     8461 gtatcattca aaagaagctc aaaaagcgtt gcaagaagat gtcaaagatg gagagaaacc
     8521 tgttaattta tctaaagatg agattaaagc aatagaaacg tcattagcaa aataaattat
     8581 attgcgtcct acaagcaaag ttcatgctta tgtttgtagg gcgttattgt ttgacaacaa
     8641 aattatttca aaagacagaa agggattgta atcattttat agttaaatat tatgaaattg
     8701 taataattta gatattgtaa aatctaataa gttgtaataa ttttaagagg taattataaa
     8761 atttgatgac acagtatatg atttttttgt aatcataatg tcatcaaaca tcaacctatt
     8821 atacataatt aaatcgtata atgatgtagt attcataaat tcggataaaa gaatgttagg
     8881 aaagttatgc aagaggagga ttttaaagtg caaaaaaaag taattgcagc tattattggg
     8941 acaagcgcga ttagcgctgt tgcggcaact caagcaaatg cggctacaac tcacacagta
     9001 aaaccgggtg aatcagtgtg ggcaatttca aataagtatg ggatttcgat tgctaaatta
     9061 aagtcattaa acaatttaac atcaaatcta attttcccta atcaagtatt aaaagtatct
     9121 ggctcaagta attccacgag caatagtagc cgtccatcaa caaattcagg tggcggatca
     9181 tactacacag tacaagcagg cgactcatta tcattaatcg catctaaata tggtacaact
     9241 taccaaaaca ttatgagact taatggttta aataatttct ttatttatcc aggtcaaaaa
     9301 ttaaaagtat caggtactgc aagctcaagt aattctgcga gcaatagtag ccgtccatca
     9361 acgaattcaa gcggtggatc atactacaca gtacaagcag gtgactcatt gtcattaatc
     9421 gcttcaaaat atggtacaac atatcaaaac attatgagac ttaatggctt aaataatttc
     9481 tttatttatc cgggtcaaaa attgaaagta actggtaatg catctacgaa ctcaggatct
     9541 actacaacga caaatagagg ttacaataca ccagtattca atcaccaaaa cttatataca
     9601 tggggtcaat gtacatatca tgtatttaat cgtcgtgctg aaattggtaa aggtattagc
     9661 acttattggt ggaatgcaaa taactgggat aacgcagcgg ctgcagatgg ttacacaatt
     9721 gacaatagac ctactgtagg ttcaatcgct caaacagatg taggctacta tggacatgtt
     9781 atgtttgtag aacgcgtaaa taacgatggt agtattttag tttcagaaat gaactattca
     9841 gctgcaccag gtattttaac ttacagaacg gtaccagctt accaagtaaa taattataga
     9901 tatattcact aaagtcttac gtatataaat atatataatg aattcctatt acatttcaca
     9961 agctgaaaag ttggattgaa tcctaaactt tatggcgtga ctatcaaagt cgtgattatt
    10021 gtaataggaa tttttgtata agaaaaagga ttgatcgaat gaacgtcatg ttctatgttc
    10081 gaccaatcct tttctgattt agtattcatg ctttactttg cgaatgcttg taaataatct
    10141 agcaccgttt gttattaaag taacaactgc cactgctttt ttgaacttgc gtggtgactt
    10201 aattgaaatg taaaagtcta acacggttcc tacagtgctt aaacctaatg aaagatatac
    10261 taatttttta tttttagcat gatatttata gccattgtag ccgtcgacta tgaaacctgc
    10321 gacatttagt aaacttgata aacgttgtga tttggaacgt tttgccataa taataattcc
    10381 ccctagtatc ttctgtttat atggaataat tgggtgcgct gaattgctaa cgttttgcgc
    10441 tataactact catatatgat aacataattg tacagtataa tttgaaaaat tgatttcaca
    10501 aagtgggggt gtcaaagatg attaaatgtg tctgtttagt agaagaaaca gctgataaaa
    10561 tattgcttgt tcaagtaagg aatcgcgaaa agtattattt cccaggtggt aaaatagaag
    10621 aaggagaatc acaagtacac gcgctgttaa gagaagtaaa agaagaaata aatttaacat
    10681 taacaatgga tgaaattgaa tatatcggga caattgtagg tcctgcatat ccacaacagg
    10741 atatgttaac tgagttaaat ggatttcgct cattaaccaa aatcgattgg gaaaatgtaa
    10801 ctatcaataa tgaaattacg gatatacgct ggattgataa agataatgat gcgttgattg
    10861 cgcctgctgt caaagtttgg attgaaacgt atggtggtaa acatgacaaa taatgacacc
    10921 atcatgttac gacattatgt cccacaagat tattcgatgt tagaagcttt tcaattaagt
    10981 gaaagtgatt tgaagtttgt taaaacgcca gaggaaaata ttacagctgc aatgtctgat
    11041 aatgaaaggt atcccatcgt tgtaatggat ggcaggcaat gtgtggcctt ttttacatta
    11101 catcgtggaa aaggagtcgc accatttagc gataaccagg atgcagtatt tttcaggtca
    11161 tttagtgttg atcaacgata tcgtaataaa ggaataggta aagtagtaat ggaaaaattg
    11221 gcgccattta tcatttcaac atttcaggat attaatgaga ttgtgttaac ggttaatact
    11281 gacaatccac atgccatggc actttatcgc caacaaggat atcaatatat gggagatagt
    11341 atgttcgtcg gaagacctgt tcatattatg gcgctaacta taaaataaat aaatttaaaa
    11401 gcatctttac tcatcgtcga ccacaaaaat taatgatgaa taaaggtgct tttgttatag
    11461 atcatcggac aatttactat agtaaaaagc gacctggtga acaattgaca tatatccaca
    11521 ggtcgcttaa cttaagttat attgctagtt gcgattaatt gatagactca tcatttttgc
    11581 gctgtcgaga tggtcttttt attaaaaatg ccgtaatcca agccgtaatc ggaatactga
    11641 ttgcaacggc aataccgcct aaaataatag aaataaattc ttgggcaaat attttcgagt
    11701 ttataatatg accaaatgaa tatttaagtt tgaaaaacca aataaataaa gcaagttggc
    11761 caccaaaaaa ggcaaggtaa atcgtgttcg cagatgtcgc taaaatttct ctaccaacac
    11821 gcatgccaga ttggaataat tcgtattgcg taagtgttgg attcacttga tgcaattcat
    11881 aaatgggtga actaatggta atcgttaagt ctatcacagc tgcaataaca gcaagaataa
    11941 tagtgaacac cataaattga accatatcaa tgccaatatt cattgagtac acatatgttt
    12001 catcttgttg ttcggttgaa aagccttgta gatgaccgaa gtagaccgat aaataaatga
    12061 gtgtaatcaa caaaattgtt gtaacgatag tgctgataaa tgcagcttgt gttttaacat
    12121 tgtaactatt gagtacgaat aaattacaag cgccaataat aatgcagaaa aagaatgtga
    12181 cgacataaat cggtacgcca aaaataatca atacaatact aataattaaa atggcaaagt
    12241 ttaaaaataa agttaaataa gagatgaatc cctttttacc tccgaaaatt atcatcagaa
    12301 agaggagcaa taacgccaat ataaatacag cattcattgt ttcgccctcc ttaatgtttc
    12361 aaatatttcc ataaacaata ttgtgatagg aattgtaagt acgataccta taccacctgt
    12421 tagtgcgcgc gcaatttcta acgaccaatt catcgaaatc gtataagtca ctgtattggc
    12481 attttttaaa aagattaaaa acataggtag tgcaccggat aaatatgaga ataataagat
    12541 gttagtcatt gttcccataa tatcttggcc tatgtttcgc ccggcaagtg cccatctcct
    12601 cattgaaatg tgcggcgtac gttgtaaaat ctcatgcatg ccacttgcaa tcgtaattgc
    12661 aacatccata atagcgccaa gtgaacctat taacactgag gctaggaaga tatctttcgg
    12721 tggtaatgat aaaaaattca tcgtttcata tttaatacct ttaccatctg tcatatatat
    12781 gattaattct gttaaaccta tactcaaaaa agttccaata attgtactgg caactgtaat
    12841 gagcgtacgc atatgccagc ctgtaacgag caataaagtg agtattgttg aacagatcat
    12901 ggcaatggtc atgagtaaga ataaattaat attactatgt tgaatatgaa tgtaaattgc
    12961 gattaatatg gcaatagaat tcaagattaa cgataaaatc gattgcagtc cgactttgcg
    13021 accaaccaat aatacagtta ataagaacaa accagtgatg ataaccgtta aggtatcacg
    13081 cttcttttct ataatataag catcactcgg cttgttagaa atatgtaata atactttttc
    13141 gtgtgtgcga aatgcttctg aatctgcttg agatttgacg tactgatgat taatcgtcgt
    13201 cgtttctcca gcaaattgac catttaatat tttgactttt aattgatttt tatacttagt
    13261 atcgcggtta ttttgtgcat cttttgtagg tgtcgatgaa acgtgtttga catctataat
    13321 ttgaccaatt ggtttgttgt aaaagttctc attattgaat gtaaataaaa tagcaccaat
    13381 gaatgcgatg cagaacaaac ctaaaattat attaaatggc tttgtaaata aatttctata
    13441 tttcaaaaac aaaaccccaa ttctatgaat gaattaatat ggtgattata cgcccttaat
    13501 tttttatttt caaagatatt actgctaagt gtaaaacgaa aatcatcatt gatagcgtcg
    13561 aattacttaa tggaatgtag acgttttagt cattaattgc tgaatgagtg ttaataagat
    13621 accaatatca ctcttcgtat aaggttcttt cgtaatagca catatcgttc tttttaattc
    13681 agtatgatct aattttatat ctatccatga tttagattct ggtaaatgta tattttgtga
    13741 tgaaatgatg taaccttctt tttgacgaag gagatactgc gcaagtggta ggctactgat
    13801 tgtgtataca tctgatttag taatcttacg caattgcttt tttacagatt cggcaaatgg
    13861 tgccaagcaa taaatatgac tatgctcaaa ctgaattaat ggtgggtgtg tcgccatcgt
    13921 aattggatcg tctgaaggcg catataaatg atagtgctct tcgaataaag gtagcatatg
    13981 taattgtttg tgtttacgta tttctggagt aagttccgtg aaaccaatgt ctatattccc
    14041 atttaatacg ctatttataa tcgtgtcatg ttctaataaa ttagggatga catgtgtatc
    14101 attttgcaaa tgaaatgttt ggataagtgg tagtaacatg tgggatacgt cactctcatc
    14161 atagccaatg tagatacttt tatttttagt taatccatgg ctttgaaatt gttcaatcgt
    14221 gctatctaaa tgttcaataa tacgcagagc ttcattaaat aataatttcc cttcagatgt
    14281 gagcataata ttgcgtcctt gttttttaaa taaagacaca ttaagttctt gttctaataa
    14341 tgtaatttga cggcttatcg ctgattgcgc aatgtttagt tcaagtgctg tttcggagat
    14401 atgttctctt ttagcgacct cgataaaata tcttaattgt ttaatttcca tagcgatata
    14461 agcacctccg aaaatgagtg ttttgtaact attatagcaa tattattggt aaatgttcta
    14521 ttttttagat gaatatcttc tattttatat attgaacaga taaatttttt agattatagt
    14581 aattatcatt aataactaat atcagaatat tctaaaaaag gggtgtgcat catgcacaat
    14641 gagaaattaa ttaaaggctt atatgactat cgtgaggaac atgatgcgtg tggtattggt
    14701 ttttatgcga atatggataa taaaaggtct cacgacatca ttgataaatc gcttgaaatg
    14761 ttgcgacgct tagatcacag gggcggggtc ggcgcagatg gcatcactgg tgatggcgca
    14821 ggtattatga ctgaaatacc ttttgcattt ttcaaacaac atgtaacgga ctttgatatc
    14881 ccaggtgaag gtgaatatgc cgtgggatta tttttttcca aagaacgcgt tttaggttct
    14941 gaacatgaag cagtttttaa aaaatatttt gaaggcgaag ggttatcaat tcttggttat
    15001 cgtaatgtac cagttaataa agatgccatt gctaaacatg tagcagatac gatgccagtc
    15061 attcaacaag tgtttattga tattagggac attgaagatg ttgaaaagcg tttgttttta
    15121 gcgagaaaac aattagagtt ctattcgact cagtgcgatt tagaattata ttttacgagc
    15181 ttatcacgca aaacaattgt atataaaggt tggttacgat cagaccaaat taaaaaactc
    15241 tatacagatt tatcggatga tttatatcaa tcaaagctag ggttagtgca ttcgagattt
    15301 agtacgaata cattcccgag ttggaaaagg gcgcatccta accgtatgtt aatgcataat
    15361 ggtgagatta acacgattaa aggtaatgtg aactggatgc gagcgcgtca acataaatta
    15421 atcgaaacat tatttggcga ggatcaacat aaagtgtttc aaattgtcga tgaggatggt
    15481 agtgactctg ccattgtaga taatgcgcta gagttcttat cgttagccat ggagccagaa
    15541 aaggcagcga tgttactcat acctgaacct tggttataca atgaagcgaa tgatgcaaat
    15601 gtacgtgcgt tttatgaatt ttatagttat ttaatggaac cgtgggatgg tcctacaatg
    15661 atttcgttct gtaacggtga caaacttggc gcgcttacag atagaaatgg attacgtcca
    15721 ggtcgttata cgattactaa agataacttt attgtctttt catctgaagt gggtgttgtg
    15781 gacgtacctg aaagtaatgt tgcttttaaa ggacaattga atcctggaaa gttattgctt
    15841 gttgatttta aacagaataa agtcattgaa aataatgatt taaaaggtgc gattgctgga
    15901 gaattaccat ataaagcgtg gattgataac cataaagttg actttgattt tgaaaatata
    15961 caatatcaag attcgcaatg gaaagatgag acgttattta aattacaacg tcagtttgca
    16021 tacacgaaag aagagattca taagtatatt caggaacttg tagaaggtaa gaaggatcct
    16081 atcggtgcaa tgggatatga tgcgccaatt gcagtgttga acgagcgacc agaatcacta
    16141 tttaattact ttaaacagct gtttgcacaa gttacgaatc caccaattga tgcgtatcgt
    16201 gaaaaaatcg taacgagtga actttcttat ttaggtggcg aaggtaactt actagcacct
    16261 gacgaaacgg ttttaaatcg tattcaattg aaaaggccgg tattgaatga atcacactta
    16321 gcagcgatta atcaggaaca ttttaaatta acttatttat caacggtata tgaaggggat
    16381 ttggaagatg cgttagaagc attaggccga gaagcagtcg atgctgtaaa gcaaggcgct
    16441 caaattctag tgttagatga tagtggatta gttgatggca atggctttgc aatgccgatg
    16501 ttactcgcaa taagtcatgt gcatcaatta cttattaaag cagatttacg tatgtctaca
    16561 agtttagtcg ctaaatctgg tgagacacga gaagtgcatc atgttgcttg tttactagca
    16621 tatggcgcga atgcaattgt gccataccta gcgcaacgta cagttgaaca actgacattg
    16681 acagaagggt tacaaggcac cgttgtcgat aatgttaaga catatacgga tgtattgtcg
    16741 gaaggtgtca ttaaagtaat ggctaagatg ggaatttcga cagtgcaaag ttatcaaggt
    16801 gcacaaatat ttgaagcgat tggcttgtct catgatgtga ttgatcgtta ttttactgga
    16861 acacagtcta agttatctgg tatttcgatt gatcaaattg atgctgaaaa taaagcacgt
    16921 caacaaagtg atgataatta tcttgcatca ggtagtacat tccaatggag acaacaaggt
    16981 caacatcatg cttttaatcc ggaatctatt ttcttattgc agcacgcatg taaagaaaat
    17041 gactatgcgc aatttaaagc atactctgaa gcggtaaaca aaaatagaac agatcacatt
    17101 agacatttac ttgaatttaa agcatgtaca ccgattgaca tcgaccaagt tgaaccggta
    17161 agtgacattg tcaaacgctt taatacaggg gcgatgagtt atggatcgat ttcagcggaa
    17221 gcacatgaaa cgttagcaca agccatgaac caattaggtg gaaagagtaa tagtggtgaa
    17281 ggtggcgaag atgcaaaacg ttatgaagta caagttgatg gaagcaacaa agtaagtgcg
    17341 attaaacaag ttgcttctgg acgttttggt gtaactagtg attatttaca acatgccaaa
    17401 gaaattcaaa ttaaagttgc gcaaggtgca aagcctggtg aaggtggtca attacctggt
    17461 actaaggtat atccgtggat tgcgaagaca agagggtcaa cgccaggtat cggtctgatt
    17521 tcatcaccgc cacatcatga tatttattca atagaagatt tagcgcaact gatacatgat
    17581 ttgaaaaatg cgaataaaga tgcagatatc gcggtaaaat tagtttcgaa aacaggtgtt
    17641 ggtaccattg catctggggt ggcaaaagca tttgcagata aaattgtcat cagtggttac
    17701 gatggtggta caggggcttc acctaaaacg agtattcagc atgccggtgt tccttgggag
    17761 attggtttag cagaaacaca tcaaacatta aaactaaatg acttaagaag tcgtgttaag
    17821 ttagaaacag acggtaagtt attaactggt aaagatgtag cgtacgcatg tgcgcttgga
    17881 gcggaagaat ttggatttgc aactgcacca ttagtggtgt tgggctgtat tatgatgcgt
    17941 gtatgccata aagatacatg tccagtagga gttgcaactc aaaacaaaga tttacgtgct
    18001 ttatatagag gtaaagcaca tcatgttgtt aattttatgc attttattgc acaagaatta
    18061 agagaaattt tagcatcttt aggtttgaaa cgtgtagaag acttagttgg aagaactgat
    18121 ttgttacaac gatcatcaac tttaaaagcg aatagcaaag cggctagtat tgatgttgaa
    18181 aaactgttat gtcctttcga tgggccaaac acaaaagaaa ttcaacaaaa tcataatctt
    18241 gagcatggat ttgatttaac aaatttatat gaagtaacga agccatatat tgctgaagga
    18301 cgtcgctata caggtagctt tacagtaaat aatgaacaac gtgatgtagg ggttattaca
    18361 ggtagtgaga tttcgaaaca atatggagaa gtaggacttc ctgaaaatac aattaatgtt
    18421 tatacgaatg gtcatgctgg tcaaagtctt gcagcatatg caccgaaagg tttaatgatt
    18481 catcatactg gagatgcgaa tgactatgtt ggtaaaggat tatctggtgg tacggttatt
    18541 gtcaaagcac cttttgaaga acgacaaaat gaaattattg ctggtaacgt ctcattctat
    18601 ggtgcgacag gtggtaaggc atttattaac ggtagtgcag gagaaagatt ctgtattaga
    18661 aatagtggtg tagatgttgt tgttgaaggt atcggcgacc atggattaga gtacatgact
    18721 ggtggacatg tcattaattt aggtgatgta ggtaagaact tcggtcaagg tatgagtggt
    18781 ggtattgctt acgttattcc gtctgatgta gaagcttttg ttgaaaataa tcaactagat
    18841 acgctttcgt ttacaaagat taaacatcaa gaagaaaaag cattcattaa gcaaatgctg
    18901 gaagaacatg tgtcacatac gaatagtacg agagcgattc atgtgttaaa acattttgat
    18961 cgcattgaag atgtcgttgt taaagttatt cctaaagatt atcaattgat gatgcaaaaa
    19021 attcatttgc acaaatcatt acatgacaat gaagatgaag cgatgttagc tgcattttac
    19081 gatgacagta aaacaatcga cgctaaacat aaaccagccg ttgtgtatta aggaaagggg
    19141 gagatacgat gggtgaattt aaaggattta tgaagtatga caaacagtac ttaggtgaat
    19201 tatcactggt agaccgtttg aagcatcata aagcatatca acaacgattt actaaagaag
    19261 atgcctctat ccaaggtgcg cgatgtatgg attgtggaac gccgttttgt caaaccggac
    19321 aacagtatgg tagggaaaca ataggttgtc caattggaaa ctacattcct gaatggaacg
    19381 acttagtgta tcatcaagat tttaaaactg cttatgaacg cttaagcgaa acaaataact
    19441 ttcctgactt tacagggcgt gtatgtcctg caccatgcga aagtgcttgt gtgatgaaga
    19501 ttaatagaga atcgattgcg attaaaggta ttgaacgcac aattattgat gaagcttttg
    19561 aaaatggttg ggtagcgccg aaagttccga gtcgccgtag agatgaaaaa gtagcaatcg
    19621 ttggaagcgg tccagcagga ttaactgctg ctgaagaact taatctacta ggatatcaag
    19681 taactattta tgaacgtgct agagaatcag gcggtttatt aatgtatggt attccgaata
    19741 tgaaacttga taaagatgtg gttcgacgtc gtattaagtt aatggaagaa gcgggcatta
    19801 ctttcattaa tggtgttgaa gtaggcgttg atattgataa agcaacgtta gaatctgagt
    19861 atgatgcaat tatattatgt actggtgcac aaaaaggtag agatttacct ttagaaggac
    19921 gcatgggtga tggtatacat ttcgctatgg attatttaac tgaacaaacg caactgttaa
    19981 atggagaaat cgatgatata acaataactg caaaagataa gaatgtcatt atcattggtg
    20041 ctggtgatac tggggcagac tgtgtagcga cagcattaag agaaaattgt aagtcgattg
    20101 ttcaatttaa taaatatacg aaattgccag aagcaattac gtttacagaa aatgcatcat
    20161 ggcctttagc aatgccggtg tttaaaatgg actatgcgca ccaagagtac gaagctaagt
    20221 ttggtaagga accacgtgca tatggtgttc aaacaatgcg ttacgatgtt gacgataaag
    20281 gacacatacg tggtttgtat actcaaattt tagagcaagg cgaaaatggt atggtcatga
    20341 aagaaggacc tgaaagattt tggcctgctg accttgtatt attatcaatc ggcttcgaag
    20401 gtacagaacc aacagtaccg aatgctttta acattaaaac ggatagaaat cgaatcgtgg
    20461 cggatgatac aaactatcaa actaataatg aaaaagtatt tgctgctgga gatgctagac
    20521 gtggtcaaag tttagttgta tgggcgatta aagaaggaag aggcgtagcg aaagcagtag
    20581 atcagtattt agcgagtaaa gtttgtgtat aatctttgta tggaaacggt ggttacgttg
    20641 acgttgaatg gtgctgaatc gagtttgaaa taatctagta tctatcaacg tcacatgcca
    20701 tctttgtaac ctaaaaataa aggtttgtaa gacaactaat atattaatta taagtagtgg
    20761 tattttacat tcgttttata ggtcaactgt agtggaagac aatgatttgt ggtaatcatg
    20821 taatgcttaa aaacaatatt gacttttaca gaacgttcat atatgataaa tattgtgttt
    20881 aggaggaata cccaagtccg gctgaaggga tcggtcttga aaaccgacag gggcttaacg
    20941 gctcgcgggg gttcgaatcc ctcttcctcc gccattaata tttatattaa atactatata
    21001 taaaaaaggt aagtactcaa agtttgagtg tttacctttt ttatttgcct ttgaatgact
    21061 cgtatttttt gataatagaa atgataaggc attgagattg gaagggcatt tggcttgtgc
    21121 aatatacata gctaaatgtc ttttttgttt tatgaaatat gatggatggc ttgtgtgggc
    21181 aagtttacta tttatagata tgcatttttc aatttaggag ttggccccat gcatctacac
    21241 tttgtaatgg cgagagcgtg gtgagaaatt gttaatagcg caattgtagt gaggagttat
    21301 tgctatatat gtcgttatgg ctcattgatt ttctgaaatg gctacaccag ataatgtgac
    21361 aaaataaaaa tattttgttg aaagccttta cataacttgt ctagacaagt tatactcgtt
    21421 ttaagacatt aagggagtga aatatatggc tgtaaaaaga gaagatgtaa aagccatcgt
    21481 aaccgctatt gggggaaaag aaaatcttga agctgcaacg cattgtgtaa cacgattacg
    21541 tttagtgctg aaggatgaaa gtaaagttga taaagacgca ttaagtaata acgcgttggt
    21601 caaggggcag ttcaaagcag accatcaata tcaaattgtc attggtccag gaacagtcga
    21661 tgaagtgtat aagcagttta ttgatgaaac aggtgctcaa gaagcttcta aagatgaagc
    21721 gaaaaaagca gctgcgcaaa aagggaatcc agtacaacgt ttgatcaaat tgttggggga
    21781 tatttttata ccaatattac ctgcgattgt gacagctggt ttgttaatgg ggattaataa
    21841 tttacttaca atgaaaggtt tatttggtcc aaaagcactt attgagatgt atccacaaat
    21901 tgctgatatt tcaaacatca ttaatgtgat tgcgagtacg gcatttattt tcttaccagc
    21961 attaattggt tggagtagta tgcgtgtatt tggtggtagt ccgattctag gcttagtctt
    22021 aggtttgatt ttaatgcacc cgcaattagt atctcagtat gatttggcaa aagggaatat
    22081 tccgacgtgg aatttatttg gcttagagat taagcagttg aattaccaag gtcaagtgtt
    22141 gcctgtttta attgcagctt atgttctagc taaaattgaa aaaggattaa ataaagtcgt
    22201 tcacgattcg ataaaaatgt tggtcgttgg acccgtagcg cttttagtta ctggattttt
    22261 agcatttatt atcattggac cagttgcatt attgattggt acaggtatta catctggtgt
    22321 tacatttata ttccaacatg caggatggct tggcggagca atatatggat tgttatatgc
    22381 accacttgta attacaggac tacaccatat gtttttagca gtagatttcc aattgatggg
    22441 tagcagctta ggtggtacgt acttatggcc aatcgttgcg atttcaaata tttgtcaggg
    22501 gtctgcagca tttggagcat ggtttgtcta taaacgtcgt aaaatggtta aagaagaagg
    22561 cttggcatta acatctggta tttctggtat gttaggtgtt actgaaccag ccatgtttgg
    22621 tgtgaactta cctctgaaat atccatttat cgctgcgata tcaacgtctt gtgtattggg
    22681 ggcaatcgtt ggtatgaata acgtacttgg aaaagttggt gttggtggcg tgccagcatt
    22741 catttcaatt caaaaagaat tttggccagt atatcttatt gtgacagcta ttgctattgt
    22801 tgtaccatgt atactaacaa ttgtgatgtc tcattttagt aaacaaaaag cgaaagaaat
    22861 tgttgaagat taataaaata aaaaaggggc gttcgttatt tggacgtcct ttattacgtt
    22921 ataaggtggt aattgtgtgt cgaaagaaat agattggaga aaatccgttg tatatcaaat
    22981 ttatcctaag tcgtttaatg atacgacggg gaatggtata ggagatatta acggaattat
    23041 agaaaaattg gattatatca agttattggg tgttgattat atttggttaa caccagtgta
    23101 tgaatcaccg atgaatgata atggctatga tatcagcgat tatttagaaa tcaatgaaga
    23161 ctttggaacg atggatgatt ttgaaaagtt aatcaaaggt gctcatcaaa aagacttgaa
    23221 agtgatgtta gatattgtca ttaatcatac gtctacggag catgaatggt ttaaagaagc
    23281 ccgtaaatct aaagacaacc catatagaga ttattacttt ttcagatcat ctgaagacgg
    23341 accgccaaca aattggcatt ctaaatttgg tggtaatgca tggaagtatg attctgagac
    23401 agatgaatat tatttacatt tatttgatgt cagtcaagct gatttaaatt gggataatcc
    23461 ggaagtacgt caatcgttat atcgcattgt caatcattgg atagacttcg gcgttgatgg
    23521 ttttcgattt gatgtcatta acttaatttc taaaggtgaa tttaaggact ctgacaaaat
    23581 aggtaaagaa ttttatacgg atggtcctag agtgcatgag tttctgcatg aattaaatcg
    23641 tcaaacgtat ggtaacactg acatgatgac tgtaggagaa atgtcttcga cgacgattga
    23701 aaattgtatt aagtatacac agccagaacg ccaagaattg aatagtgttt ttaattttca
    23761 tcatctaaag gttgattatg ttgatggtga aaagtggaca aatgcgaagc ttgattttca
    23821 taagttaaag gaaattctga tgcaatggca acgaggtatt tatgacggtg gcggatggaa
    23881 cgcgattttc tggtgtaatc atgatcagcc acgggtagtg tctagatttg gtgatgatac
    23941 gtcggaagag atgaggatac aaagtgctaa aatgttagct atcgcactgc atatgttgca
    24001 aggtacgcca tatatttacc aaggtgaaga aattggtatg acggacccac attttacatc
    24061 aatagcacaa tatcgtgatg ttgaatcgat taatgcctac catcagttgt taagtgaagg
    24121 gcatgctgaa gcggatgtgt tagcgatttt aggacagaag tcacgagaca attcgagaac
    24181 gcctatgcaa tggagtgatg atgttaatgc tggatttaca gctggtaagc cttggattga
    24241 tattccagaa aattatcatc aggtcaacgt tagacaagca ctaaaggata aagattctat
    24301 tttctacacg tatcaaaaat taatacaatt aagacatacg catgatatta ttacgtatgg
    24361 agacattgtg ccacgtttta tggatcatga tcatttattt gtttatgaac gtcattataa
    24421 gaatcaacaa tggctagtaa ttgcgaattt ctcagcatcg gctgttgatt tgccagaagg
    24481 attggctagc gaaggtcgtg ttgtgattca aacaggcaca gtggaaaata atacgataag
    24541 cgggtttggt gcaattgtaa tcgaaacaaa cgcgtaaaat aaattgagtg gatgcgttta
    24601 tatggcgaaa caaaaaaagt ttatgaagat ttatgaggcg ttgaaagaag atatattaaa
    24661 cgggcagatt caatatggtg aacaaattcc gtctgaacat gatttggtgc aattgtacca
    24721 gtcatctcga gagaccgtgc gtaaggcatt agatttgttg gcattagacg gtatgattca
    24781 aaagattcat ggtaaagggt cgcttgtcat ttatcaggag gttacagagt ttccattttc
    24841 tgaacttgtt agttttaaag aaatgcaaga agaaatgggt gtcgcatatt taactgaagt
    24901 tgttgtgaat gaggttgttg aagcgcatga agttccagaa gttcaagatg ctttaaacat
    24961 caattctagt gaatcgctca ttcatattgt tagaactcgt cggcttaacc aacatgtgaa
    25021 gattgttgat gaagattatt ttctaaagtc gattgtttca gacataggga atgatgttgc
    25081 gagtgattct atttatgatt atttggaaaa ggtattaaat cttaatatta gttattcaag
    25141 taagtctatt acttttgaac cgtttgatga acaagcatat caattgtttg gtgatgtatc
    25201 gttggcttat tcagcaacag ttcgaagtat tgtgtattta gaaaatacaa tgccgtttca
    25261 atataatatt tcaaaacatc ttgcaaatga atttaaattt aacgacttct caagacgtcg
    25321 tataaagtaa acaatgatat aaatgattta tacttgcaat taactattaa aatatagtaa
    25381 tatatatctt gccgtgctag gtggggaggt agcggttccc tgtactcgaa atccgcttta
    25441 tgcgaggctt aattcctttg ttgaggccgt atttttgcga agtctgccca aagcacgtag
    25501 tgtttgaaga tttcggtcct atgcaatatg aacccatgaa ccatgtcagg tcctgacgga
    25561 agcagcatta agtggatcat catatgtgcc gtagggtagc cgagatttag ctaacgactt
    25621 tggttacgtt cgtgaattac gttcgatgct taggtgcacg gttttttatt tttttaaata
    25681 ttaaaccgat tattaagagt tgaaaatata tatttataga agctactttc ttgaagacaa
    25741 ttcagcgtat tatacgtgga acatgtttgt gggaagtagc ttttttatat gtgaagtttg
    25801 attcaagtga aatcgatgcg cagtttgaat gattttttac ggatataaaa agtaagaagt
    25861 tattatttga tgataaagaa atgatggtga aatgaggggg agtatcttac aatagaatta
    25921 ttaatgagat acgttatgat tattgacaat caaatgccta cggaggacat atgcaaatat
    25981 atttaagtac tttaacagag ttagattatg ataaatcttt aaatagtatt gaagaaagtt
    26041 ttgatgataa tcctgaaacg agttggcaag cacgtgcgaa agtaaaacat ttaagaaaat
    26101 ctccttgcta taattttgaa ttagaagtaa ttgcgaaaaa tgaaaataac gatgtcgttg
    26161 gacacgtttt attaattgaa gtagaaatta atagtgatga taagacgtat tatggtttgg
    26221 caattgcttc tttatcagtt catcctgaat tgcgtggaca aaaattaggt cgtggcttgg
    26281 ttcaagcagt agaagagcgt gcaaaagcgc aagagtatag tacggttgtt gtagaccatt
    26341 gttttgacta ctttgaaaag ttgggttatc aaaatgctgc tgagcatgac attaaattag
    26401 aatctggtga tgcaccgtta cttgtaaaat atttatggga taatttgacg gatgcaccac
    26461 acggaatcgt aaaatttcca gaacattttt attaattgtt catttaagaa gtaaaggtat
    26521 tatcatgcta taatgaaagg taattgttta tggaggtgct aacttgaatt atcaagcctt
    26581 atatcgtatg tacagacccc aaagtttcga ggatgtcgtc ggacaagaac atgtcacgaa
    26641 gacattgcgc aatgcgattt cgaaagaaaa acagtcgcat gcatatattt ttagtggtcc
    26701 gagaggtacg gggaaaacga gtattgccaa agtgtttgct aaagcaatca actgtttaaa
    26761 tagcactgat ggagaacctt gtaatgaatg tcatatttgt aaaggcatta cgcaggggac
    26821 taattcagat gtgatagaaa ttgatgctgc tagtaataat ggcgttgatg aaataagaaa
    26881 tattagagac aaagttaaat atgcaccaag tgaatcgaaa tataaagttt atattataga
    26941 tgaggtgcac atgctaacaa caggtgcttt taatgccctt ttaaagacgt tagaagaacc
    27001 tccagcacac gctattttta tattggcaac gacagaacca cataaaatcc ctccaacaat
    27061 catttctagg gcacaacgtt ttgattttaa agcaattagc ctagatcaaa ttgttgaacg
    27121 tttaaaattt gtagcagatg cacaacaaat tgaatgtgaa gatgaagcct tggcatttat
    27181 cgctaaagcg tctgaagggg gtatgcgtga tgcattaagt attatggatc aggctattgc
    27241 tttcggcgat ggcacattga cattacaaga tgccctaaat gttacgggta gcgttcatga
    27301 tgaagcgttg gatcacttgt ttgatgatat tgtacaaggt gacgtacaag catcttttaa
    27361 aaaataccat cagtttataa cagaaggtaa agaagtgaat cgcctaataa atgatatgat
    27421 ttattttgtc agagatacga ttatgaacaa aacatctgag aaagatactg agtatcgagc
    27481 actgatgaac ttagaattag atatgttata tcaaatgatt gatcttatta atgatacatt
    27541 agtgtcgatt cgttttagtg tgaatcaaaa cgttcatttt gaagtgttgt tagtaaaatt
    27601 agctgagcag attaagggtc aaccacaagt gattgcgagt gtagctgaac cagcacaaat
    27661 tgcttcatcg ccaaacacag acgtattgtt gcaacgtatg gaacagttag agcaagaact
    27721 aaaaacacta aaagcacaag gagtgagtgt cgctcctact caaaaatctt cgaaaaagcc
    27781 tgcgagaggc atacaaaaat ctaaaaatgc attttcaatg caacaaattg caaaagtgct
    27841 agataaagcg aataaggcag atatcaaatt gttgaaagat cattggcaag aagtgattga
    27901 tcatgccaaa aacaatgata aaaaatcact cgttagttta ttgcaaaatt cggaacctgt
    27961 ggcggcaagt gaagatcacg tacttgtgaa atttgaggaa gagatccatt gtgaaatcgt
    28021 caataaagac gacgagaaac gtagtagtat agaaagtgtt gtatgtaata tcgttaataa
    28081 aaacgttaaa gttgttggtg taccatcaga tcaatggcaa agagttcgaa cggagtattt
    28141 acaaaatcgt aaaaacgaag gcgatgatat gccaaagcaa caagcacaac aaacagatat
    28201 tgctcaaaaa gcaaaagatc ttttcggtga agaaactgta catgtgatag atgaagagtg
    28261 atacatgaca agcgatataa tcgtatgtat aatgaaagaa acatcatttt attgataaat
    28321 atttattgat tttcaaggag gaaatggaat atgcgcggtg gcggaaacat gcaacaaatg
    28381 atgaaacaaa tgcaaaaaat gcaaaagaaa atggctcaag aacaagaaaa acttaaagaa
    28441 gagcgtattg taggaacagc tggcggtggc atggttgcag ttactgtaac tggtcataaa
    28501 gaagttgtcg acgttgaaat caaagaagaa gctgtagacc cagatgatat tgaaatgcta
    28561 caagacttag tgttagcagc tactaatgaa gcgatgaata aagctgatga gcttactcaa
    28621 gaacgtttag gtaaacatac tcaaggctta aacatccctg gaatgtgatt atagatgcat
    28681 tatccagaac ctatatcaaa gcttattgat agctttatga aattgccagg cattggtcca
    28741 aagacagccc aacgtctggc ttttcatacc ttagatatga aagaagacga tgttgttcag
    28801 tttgccaaag cattagtaga tgttaaaaga gaattaacat attgtagcgt atgtggtcac
    28861 attactgaaa atgatccatg ttatatttgt gaagataagc aaagagatcg ttcagttatt
    28921 tgtgttgtgg aagatgacaa agatgtcata gctatggaaa aaatgagaga atacaaaggt
    28981 ttatatcacg ttttacatgg gtctatttcc cctatggatg gcattggacc agaagatatt
    29041 aatattcctt cattgattga acgcttgaaa aacgatgaag ttagcgaatt aatcttagct
    29101 atgaacccga acttagaagg ggaatctaca gccatgtata tttctagatt agttaagcct
    29161 ataggtatca aagtgacgag attagcacaa gggttatcgg taggtggcga tttagagtat
    29221 gctgacgaag taacattatc taaagcaatc gcaggtagaa cagaaatgta atgtcttcta
    29281 ttaaacattt tagaatttaa tactatagta agaaaagtca cagtgtaatc attgtggctt
    29341 tttttatggt gtggtgtact actttatttg tggtgtggcg gtggcatggt ttacctagtt
    29401 ttactgaggg gtggggaatc tttatgaagc aaaccattgg ttgtgatttg ttatttctaa
    29461 tagtaatgaa gtgaattgga ttatcgaagt agatctacgg ttgtggtgtg ttggtgctat
    29521 taatttgata aatgcggttg atggttaatg actaggcaaa tgaaattctt ttgtaattga
    29581 aatgatagat gctgacttag tagttgtact tatttggtct aaagcttatt aaatcagcct
    29641 gtatagtggt gttttgagag attatttaaa acttgtaaat ttatttttaa tttctggtaa
    29701 aaaaataaag ttctgttttg cggttttttt attatctttt tataaaaata accaccttaa
    29761 ttgttttaga aaacgtacta ttcacaattg taatttcttt aagttcgatt tttagaatga
    29821 gagcgttcgg aatggatagt aaaggtgtaa tttttactgt tgttaagcag tttgaaagcc
    29881 tgtatagtat ttatttgttg aggcaaacaa aacaactcaa cttaagaaat aacttgaatt
    29941 actaacgaaa attaatttta aaaagttatt gacttaaatg ttaataaaat gtataattaa
    30001 ttcttgtcgg taagaaaaat gaacattgaa aactgaatga caatatgtca acgttaattc
    30061 caaaaaacgt aact
//
LOCUS       DAPIDM010000018        29515 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000018 DAPIDM010000000
VERSION     DAPIDM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.018"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(16..243)
                     /locus_tag="RJE42_000715"
     CDS             complement(16..243)
                     /locus_tag="RJE42_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876162.1"
                     /translation="MKMRLQQAFFNSSQGPQQREIGFPISTGNASWGGAPTQRISKRN
                     STSNASWGVGPNTEADEKSAYDNVQVGAGQR"
     gene            438..1349
                     /gene="rapZ"
                     /locus_tag="RJE42_000716"
     CDS             438..1349
                     /gene="rapZ"
                     /locus_tag="RJE42_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV5876163.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1346..2341
                     /locus_tag="RJE42_000717"
     CDS             1346..2341
                     /locus_tag="RJE42_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDV5876164.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2450..3394
                     /gene="whiA"
                     /locus_tag="RJE42_000718"
     CDS             2450..3394
                     /gene="whiA"
                     /locus_tag="RJE42_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDV5876165.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3964..4551
                     /gene="clpP"
                     /locus_tag="RJE42_000719"
     CDS             3964..4551
                     /gene="clpP"
                     /locus_tag="RJE42_000719"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV5876166.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            4734..6368
                     /locus_tag="RJE42_000720"
     CDS             4734..6368
                     /locus_tag="RJE42_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDV5876167.1"
                     /translation="MKGIELLNNPFLNKGIAFTNEERKQLGLEGLLPANVRTLEQQAE
                     QCYEQFKAKQTDFEKRLFLMAIFNRNRTLFYKLTSEHLVEFMPIIYDPVIAQSIEQYN
                     ENFSRPQDAVFLSVDRQDQIEQELRNVANGRDIRLIVVTDAEGILGIGDWGVDGVDIA
                     IGKLIVYTAAAGINPSQVLPVSLDVGTNNEQLLNDDLYLGNRHKRVDDETYYAFVDKF
                     VSAVRKEYPNALLHWEDFGRGHAKNILDKYEDTLPTFNDDIQGTGIVTLAGVLGALNI
                     SKVNYTNQTFLVYGGGTAGMGITNILKDELIKQGVSEEKANQHFYIMDKQGLLFDDMD
                     DLTEAQQVFAKNRNEFSKTVNWKNLKEVIENIKPTVLIGTSTQTGAFTEDAVKEMLKH
                     TERPMIFPLSNPTKLAEATAQDLLKWTNGKALIGTGIPYNDIEYNGVNYSIGQANNAL
                     MYPGLGLGLIASKAKKVNQQILSAASHALGGMVNPDEPGAAVLPPVEKIHQCSRAIAK
                     AVAQSVIDQGLNAETIDDIDKAIEAEIWYPEYKSYI"
     gene            complement(6575..7477)
                     /locus_tag="RJE42_000721"
     CDS             complement(6575..7477)
                     /locus_tag="RJE42_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV5876168.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQKIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLISK"
     gene            8180..8809
                     /locus_tag="RJE42_000722"
     CDS             8180..8809
                     /locus_tag="RJE42_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDV5876169.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQNQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            9584..10597
                     /locus_tag="RJE42_000723"
     CDS             9584..10597
                     /locus_tag="RJE42_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDV5876170.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            10650..11660
                     /gene="gap"
                     /locus_tag="RJE42_000724"
     CDS             10650..11660
                     /gene="gap"
                     /locus_tag="RJE42_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV5876171.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            11799..12989
                     /locus_tag="RJE42_000725"
     CDS             11799..12989
                     /locus_tag="RJE42_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV5876172.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPDLGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            13111..13872
                     /locus_tag="RJE42_000726"
     CDS             13111..13872
                     /locus_tag="RJE42_000726"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV5876173.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEEQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            13875..15392
                     /locus_tag="RJE42_000727"
     CDS             13875..15392
                     /locus_tag="RJE42_000727"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV5876174.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            15522..16826
                     /gene="eno"
                     /locus_tag="RJE42_000728"
     CDS             15522..16826
                     /gene="eno"
                     /locus_tag="RJE42_000728"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV5876175.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGKHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            17165..17623
                     /locus_tag="RJE42_000729"
     CDS             17165..17623
                     /locus_tag="RJE42_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876176.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            17690..17923
                     /gene="secG"
                     /locus_tag="RJE42_000730"
     CDS             17690..17923
                     /gene="secG"
                     /locus_tag="RJE42_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV5876177.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            18052..18792
                     /locus_tag="RJE42_000731"
     CDS             18052..18792
                     /locus_tag="RJE42_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDV5876178.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMHHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQAENDNMIDPQSANYIYEHVDSDEKDIKWYSQS
                     GHVITIDKEKEQVFDDIYQFLESLDWSE"
     gene            18826..21198
                     /gene="rnr"
                     /locus_tag="RJE42_000732"
     CDS             18826..21198
                     /gene="rnr"
                     /locus_tag="RJE42_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV5876179.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDKDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSQARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEATPDNIEDSEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPTIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGRQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            21220..21684
                     /gene="smpB"
                     /locus_tag="RJE42_000733"
     CDS             21220..21684
                     /gene="smpB"
                     /locus_tag="RJE42_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV5876180.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            21775..22136
                     /gene="ssrA"
                     /locus_tag="RJE42_000734"
     tmRNA           21775..22136
                     /gene="ssrA"
                     /locus_tag="RJE42_000734"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22196..23245)
                     /locus_tag="RJE42_000735"
     CDS             complement(22196..23245)
                     /locus_tag="RJE42_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV5876181.1"
                     /translation="MASFTITKRKNKATASWQYDVKHPSFKSGKKRKSGFKTKAEATN
                     AAQQLLRDLDDGNNLDENKKFEEYYNDWLDSKNKKHVASQQFYWYQRSLKLFNEYFGS
                     DFLIKNIKRSEYQKFLNEFGRGRTNETVRKLNSCLSQCFRDAVYEGYLKKDPSYQIDI
                     RGTKKSKDEHSKFITIEQYLQLIEYFKSRDESSYIFLFILAITGARYSDVINMLPIDL
                     NEKEGTIHLRGTKTVNADRLVEVPKKDIQHIKAKLAKLPKRTDNKLFKLSHNAVKKSF
                     NHAKSQIGLNDTKITPYSLRHTHTSFLLSKGIPIEYISKRLGHYNISITLDTYSHLLD
                     EHKKEQGQRVRKLFS"
     gene            complement(23307..23822)
                     /locus_tag="RJE42_000736"
     CDS             complement(23307..23822)
                     /locus_tag="RJE42_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876182.1"
                     /translation="MKRLLYLVLASTLVLSACGSNDGDKKEENKKTETKKENKNKKKV
                     TKDKAESKKENANQNDNNNQVNNENNTNVNDHQQTNNAPKQNQTQNNPASNKKNNAPV
                     KDEFSSDTSYNAYQEAKRATEENKRQNGGHTAGIGGSWAVQDGQDYNSWKKAQNDFDN
                     FKRQNSEVIQQ"
     gene            complement(23826..24317)
                     /locus_tag="RJE42_000737"
     CDS             complement(23826..24317)
                     /locus_tag="RJE42_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876183.1"
                     /translation="MNQVPNDKLTVKESWTAGKIRGKLNKGQKQVFDRMSISEKRDII
                     EKFNNNIPFEVEEIERNQETKYKIIEKTLNKRELNTMSESGKDMLLKNKVGQFIDSFS
                     TRFSSSFSNPNNAGQMFTYEMINQNFVLIEMLDEHLKNENKIIEQNNEIIDLLKQIAN
                     KGV"
     gene            complement(24338..24796)
                     /locus_tag="RJE42_000738"
     CDS             complement(24338..24796)
                     /locus_tag="RJE42_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV5876184.1"
                     /translation="MGKYEELLMKCEVEVKETQRVPRGFDGWYQEGEIFIRPSLSERN
                     KLEVLYEELAHHKLTYGNILDQSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            complement(24818..25144)
                     /locus_tag="RJE42_000739"
     CDS             complement(24818..25144)
                     /locus_tag="RJE42_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5876185.1"
                     /translation="MEYKSARKILSENLEQLMKENNITQVELSEAIGVSQSTISNWLK
                     ELKYPRISKVQQLADYFNVPKSRITEERSIHQETIAGHANKDEFTPEEWEEIENFMQW
                     VRDRKK"
     gene            25307..25516
                     /locus_tag="RJE42_000740"
     CDS             25307..25516
                     /locus_tag="RJE42_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5876186.1"
                     /translation="MPENFKEFSVKVWRTNSNMTQQDVADKLGVTKQSVIRWEKDDAE
                     LKGLQLYALAKLFNTEVDYIKAKKI"
     gene            25555..26280
                     /locus_tag="RJE42_000741"
     CDS             25555..26280
                     /locus_tag="RJE42_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDV5876187.1"
                     /translation="MQELQTFNFEELPVRKIEVEGEPFFLGKDVAEILGYARADNAIR
                     NHVDSEDRLMHQISASGQNRNMIIINESGLYSLIFDASKQSKNENIRETARKFKRWVT
                     SEVLPTLRKTGAYQIPSDPMQALRLMFEATEQTKQEIKNVKDDVIDLKENQKLDAGDY
                     NFLTRTINQRVAHIQRLHAITNQKQRSELFRDINSEVKKMTGASSRTNVRQKHFDDVI
                     EMIANWFPSQATLYRIKQIEMKF"
     gene            26305..26514
                     /locus_tag="RJE42_000742"
     CDS             26305..26514
                     /locus_tag="RJE42_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876188.1"
                     /translation="MEYIGYADANAFVKISGISKDDLEKKVYSNKEFQKECMYRFGRG
                     QKRYIKIDKAIQFIGTNLMINEYEL"
     gene            26527..26688
                     /locus_tag="RJE42_000743"
     CDS             26527..26688
                     /locus_tag="RJE42_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDV5876189.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            26780..27040
                     /locus_tag="RJE42_000744"
     CDS             26780..27040
                     /locus_tag="RJE42_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDV5876190.1"
                     /translation="MYYEIGDVCQKVINVDGFDFKLAVKKKDHSILVNILDLEDKFID
                     GINITNENDLYTALDILNQSIYEWIEENADDYDRLINLVMKW"
     gene            27048..27284
                     /locus_tag="RJE42_000745"
     CDS             27048..27284
                     /locus_tag="RJE42_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876191.1"
                     /translation="MKPHKFKRMAIDLIERVQSTSYQVDYKYNVIWVWHYSDDYLGKV
                     ASINMHNNVDDDNTILARYEKAKKMLAGEVLSDG"
     gene            27277..27756
                     /locus_tag="RJE42_000746"
     CDS             27277..27756
                     /locus_tag="RJE42_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDV5876192.1"
                     /translation="MANLYELSEAFKELSNQDELDQTLLKDTLDSIQAEMNVKVDNIV
                     NWRRETLGDIDVIDKEIKRLQNLKKQKQNLTDRLRDYLKEMLETQEVDSYRTATNHIF
                     KRKNGASKNIIDEKLIPKDYWLSQAPKLNSKQLIDDLKAGKDIPGVELKVTESLVIK"
     gene            27756..28394
                     /locus_tag="RJE42_000747"
     CDS             27756..28394
                     /locus_tag="RJE42_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV5876193.1"
                     /translation="MNKSETVVEINKAMVAFRKEVKQPLKDKNNPFFKSKYVPLENVV
                     EAIDEAATPHGLSYTQWALNDVDGRVGVATMLMHESGEYIEYDPVFMNAEKNTPQGAG
                     SLISYLKRYSLSAIFGITSDQDDDGNEASGKNNNPKQQTRTQWASSETISVLRKEVID
                     FTNLIKGTDKEAPQNIVEQKFDINNYKLTEKQAAEAIQKIRNNAKTITGGKQ"
     gene            28394..28822
                     /gene="ssb"
                     /locus_tag="RJE42_000748"
     CDS             28394..28822
                     /gene="ssb"
                     /locus_tag="RJE42_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV5876194.1"
                     /translation="MLNRTVLVGRLTKDPEYRTTPNGVSVTTFTIAVNRTFTNAQGER
                     EADFINCVTFRKQAENVNNYLSKGSLAGVDGRLQSRSYENKDGQRVFVTEVVADSVQF
                     LEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAIIDDDLPF"
     gene            28836..29510
                     /locus_tag="RJE42_000749"
     CDS             28836..29510
                     /locus_tag="RJE42_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV5876195.1"
                     /translation="MPIITSYITQDDGTTTVVISGVELGNKETLLLDNGFDVEVDVSV
                     IDPFRITGKQRRKIFALVKDIEEYTGQPMDYMRHMFIEYVRTYYGYDERISLSNCTRT
                     QASQIIEATLDWTFYNDIPLSYKTSNLLKRDKSFLYWSTVNRNCVICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCRQHHNEQHAIGVKSFDDKYQLHDSWIKVDERLNKML
                     KGEKNE"
BASE COUNT        10420 a         4451 c         5573 g         9071 t
ORIGIN      
        1 agaaaaagta tttctttatc gttgtccggc cccaacttgc acattatcgt aagctgactt
       61 ttcgtcagct tctgtgttgg ggcccacacc ccaactcgca ttgcttgtag aatttctttt
      121 cgaaattctc tgtgttgggg ccccacccca actcgcattg cctgtagaaa ttgggaatcc
      181 aatttctctt tgttggggcc cctgactaga gttgaaaaaa gcttgttgca agcgcatttt
      241 cattcggtca actactagca atataatatt atagacccta ggacattgat ttatgtccca
      301 agctcctttt aaatgatgta tatttttaga aatttaatct agacatagtt aaaaataaat
      361 ataaaacatc gttgcttaat tttgtcatag aacatttaaa ttaacatcat gaaattcgtt
      421 ttggcggtga aaaaataatg gataataatg aaaaagaaaa aagtaaaagt gaactattag
      481 ttgttacagg tttatctggc gcaggtaaat ctttggttat tcaatgttta gaagacatgg
      541 gatatttttg tgtagataat ctaccaccag tgttattgcc taaatttgta gaattgatgg
      601 aacaaggaaa tccatcctta agaaaagtgg caattgcaat tgatttaaga ggtaaggaac
      661 tatttaattc attagttgca gtagtggata aagtcaaaag tgaaagtgac gtcatcattg
      721 atgttatgtt tttagaagca agtactgaaa aattaatttc aagatataag gaaacgcgtc
      781 gtgcacatcc tttgatggaa caaggtaaaa gatcgttaat caatgcaatt aatgatgagc
      841 gagagcattt gtctcaaatt agaagtatag ctaattttgt tatagatact acaaagttat
      901 cacctaaaga attaaaagaa cgcattcgtc gatactatga agatgaagag tttgaaactt
      961 ttacaattaa tgtcacaagt ttcggtttta aacatgggat tcagatggat gcagatttag
     1021 tatttgatgt acgattttta ccaaatccat attatgtagt agatttaaga cctttaacag
     1081 gattagataa agacgtttat aattatgtta tgaaatggaa agagacagag attttctttg
     1141 aaaaattaac tgatttgtta gattttatga tacccgggta taaaaaagaa gggaaatctc
     1201 aattagtaat tgccatcggt tgtacgggtg gacaacatag atctgtagca ttagcagaac
     1261 gactaggtaa ttatctaaat gaagtatttg aatataatgt ttatgtgcat catagagacg
     1321 cacatattga aagtggcgag aaaaaatgag acaaataaaa gttgtactta tcggtggtgg
     1381 cactggctta tcagtcatgg ctaggggatt aagagaattc ccaattgata ttacagcgat
     1441 tgtaacagtt gctgataatg gtgggagtac agggaaaatc agagatgaaa tggatatacc
     1501 agcaccagga gacatcagaa atgtgattgc agctttaagt gattctgagt cagttttaag
     1561 ccaacttttt cagtatcgtt ttgaagaaaa tcaaattagc ggtcactcat taggtaattt
     1621 attaatcgca ggtatgacta atattacgaa tgatttcgga catgccatta aagcattaag
     1681 taaaatttta aatattaaag gtagagtcat tccatctaca aatacaagtg tgcaattaaa
     1741 tgctgttatg gaagatggag aaattgtttt tggagaaaca aatattccta aaaaacataa
     1801 aaaaattgat cgtgtgtttt tagaacctaa cgatgtgcaa ccaatggaag aagcaatcga
     1861 tgctttaagg gaagcagatt taatcgttct tggaccaggg tcattatata cgagcgttat
     1921 ttctaactta tgtgtcaatg gtatttcaga tgcgttaatt cattctgatg cgcctaagct
     1981 atatgtatct aatgtgatga cgcaacctgg ggaaacagat ggttatagcg tgaaagatca
     2041 tatcgacgcg attcatagac aagctggaca accgtttatt gattatgtca tttgtagtac
     2101 tcaaactttc aatgctcaag ttttgaaaaa atatgaagaa aaacattcta aaccagttga
     2161 agttaataag gctgaacttg aaaaagaaag cataaatgta aaaacatctt ctaatttagt
     2221 tgaaatttct gaaaatcatt tagtaagaca taatactaaa gtgttatcga caatgattta
     2281 tgacatagct ttagaattaa ttagtactat tcctttcgta ccaagtgata aacgtaaata
     2341 atatagaacg taatcatatt atgatatgat aatagagctg tataaaaatg aaaatagaca
     2401 gtggttctaa ggtgaatcat gttttaaata agaaaggaat gactgtacga tgagctttgc
     2461 atcagaaatg aaaaatgaat taactagaat agacgtcgat gaaatgaatg caaaagcaga
     2521 gctcagtgca ctgattcgaa tgaatggtgc acttagtctt tcaaatcaac aatttgttat
     2581 aaatgttcaa acggaaaatg caacaacggc aagacgtatt tattcgttga ttaaacgtgt
     2641 atttaatgtg gaagttgaaa tattagtccg taaaaaaatg aaacttaaaa aaaataatat
     2701 ttatatttgt cgtacaaaga tgaaagcgaa agaaattctt gatgaattag gaattttaaa
     2761 agacggcatt tttacgcatg aaattgatca ttcaatgatt caagatgacg aaatgagacg
     2821 cagttacttg agaggagctt ttctggcagg tggctcagtg aataaccctg aaacatcttc
     2881 gtatcatttg gaaatttttt ctcaaaatga gagtcatgca gaaggcttaa cgaaactaat
     2941 gaatagttat gagttgaatg ccaaacattt agagcgaaaa aaaggaagta ttacgtattt
     3001 aaaagaagcg gaaaagattt cggattttct tagtttgata ggtggctatc aagcgttatt
     3061 aaaatttgaa gacgtacgca ttgtaagaga tatgcgtaat tctgttaacc gactcgttaa
     3121 ttgtgaaacg gcaaatctaa ataaaactgt tagtgctgcg atgaaacaag tggagagcat
     3181 taaattaatt gataaagaaa ttggtattga aaatttacca gacaggttga gagagattgc
     3241 tagaattcga gtagaacatc aagaaatttc gttgaaagag cttggagaaa tggtgtcaac
     3301 tggtccaatt tcaaaatcag gtgtaaatca tcgattaaga aaacttaatg atttagccga
     3361 taagattaga aatggtgaac aaatagaatt ataagtaaga aggtgttttt ggtagtgaat
     3421 tatcaaaaat acctttttta tgattaaaaa gtgtttaatg aataatagtc gactaactat
     3481 taaattgagc tggaaagttt aatgaaggaa attcaataac caagcgttac ctaattttct
     3541 cattaagtag catcatattt atcaatttgg tcataagaaa atacgaatgt gataattaat
     3601 tgaatccctg aaagtccctg attatagaca aaaaaatcag cataaaaaga atcccgacat
     3661 tgcgggattc tctgtattga aaagtattgt attttattta aatgcctcct tgaagggaat
     3721 cgaaccccta tcttaagaac cggaatctta cgtgttatcc attacaccac aaggagtaaa
     3781 tatgttaatc aaatcactgt actatgagaa gtaaaataag tacaatctaa aatagatatt
     3841 atcagtttac aaaggaaaga gacaagcgtc aaacaaagta gcgttttaaa gtcaaagtgt
     3901 ttgaccaaat ttgacttaat atgtaaaata atgagtaaca gttattacaa ggaggaaata
     3961 tagatgaatt taattcctac agttattgaa acaacaaatc gcggtgaacg tgcatacgat
     4021 atatactcac gtttattgaa agaccgtatt attatgttag gttcacaaat tgatgacaac
     4081 gtagcaaatt caatcgtatc acagttatta ttcttacaag cacaagactc agagaaagat
     4141 atttatttat acattaattc accaggtgga agtgtaacag ctggttttgc gatttatgat
     4201 acgattcaac acattaaacc tgatgttcaa acaatttgta tcggtatggc tgcatcaatg
     4261 ggatcattct tattagcagc tggtgctaaa ggtaaacgtt tcgcgttacc aaatgcagaa
     4321 gtaatgattc accaaccatt aggtggtgct caaggacaag caactgaaat tgaaattgct
     4381 gcaaatcaca ttttaaaaac acgtgaaaaa ttaaaccgca ttttatcaga gcgtactggt
     4441 caaagtattg aaaaaataca aaaagacaca gatcgtgata acttcttaac tgcagaagaa
     4501 gctaaagaat atggcttaat tgatgaagtg atggtacctg aaacaaaata attcaaagta
     4561 aagagtagac taagcagtct gctctttttt gtatgagtaa accaaggtgt caataaattg
     4621 ttcaaaacat tttgagtaaa agcaaaaact aacgggtaaa aatatttgtg agaatttaat
     4681 tagacacgtt gcgtctgcgg ttctacaatc taacaaatat aaaggatgat tttatgaaag
     4741 gcattgaatt acttaacaat ccatttttaa acaaaggtat cgcttttact aacgaagaaa
     4801 gaaaacagtt agggcttgaa ggtttattac ctgcgaatgt tcgaacttta gaacaacaag
     4861 cagaacaatg ttatgaacaa ttcaaggcga aacaaaccga ttttgagaaa cgtttatttt
     4921 taatggcaat ttttaatcgt aatcgtacat tgttttacaa attgacttca gagcatttag
     4981 ttgaatttat gccaattatt tatgaccctg ttattgcaca atcgattgaa caatataatg
     5041 agaatttcag cagaccccaa gatgcagtat ttttatctgt tgatagacaa gaccaaattg
     5101 aacaagaatt gcgcaacgtt gcaaatggca gagatattcg attaatcgta gtgacagatg
     5161 cggaaggtat attaggtatc ggcgattggg gcgttgatgg tgtagatatt gcgataggaa
     5221 aattaatagt atatacagcg gcagctggaa taaatccctc acaagtatta cctgtatctt
     5281 tagatgtagg tactaataat gaacaattac tgaatgatga cctttattta ggaaatcgtc
     5341 ataaacgtgt cgatgatgag acatattacg catttgtaga taaatttgta agtgcagtac
     5401 gtaaagaata tcctaatgca ctattacatt gggaagattt tggtagaggg catgccaaaa
     5461 atattttaga caaatatgaa gatacgttac caacttttaa tgatgatatt caaggtacag
     5521 gtatagttac attagctggc gtactaggtg cattaaatat ttctaaagta aattatacga
     5581 atcagacgtt tttagtttat ggtggtggta cagctggtat gggtattacc aatattttaa
     5641 aagatgaact aattaaacag ggtgtttcag aagaaaaagc aaatcagcat ttctacatta
     5701 tggataaaca aggattgttg tttgatgata tggatgattt aactgaagcg caacaagtat
     5761 ttgctaaaaa taggaatgaa ttttcaaaga cagtaaactg gaaaaattta aaagaagtaa
     5821 ttgaaaatat taagcctact gttttaatcg gtacatcaac tcaaacaggt gcatttacag
     5881 aagatgcagt aaaagagatg ttaaagcata cagaacgtcc aatgattttc ccattgtcta
     5941 atcctacaaa attagcagaa gcaactgcac aagatttatt aaaatggact aacggaaaag
     6001 cattaattgg aactggtatc ccatataatg acattgaata caatggtgtg aattacagta
     6061 ttggtcaagc aaataatgca ttgatgtatc ctggtttagg tttgggtctt attgcgtcaa
     6121 aagcgaagaa agtaaatcaa cagattctat cagctgcgag tcatgcactt ggtggcatgg
     6181 tcaatccaga tgaaccagga gctgcagtct taccacctgt tgagaaaata catcaatgtt
     6241 ctcgtgccat agccaaagcg gttgcacaaa gtgtcattga tcaaggatta aacgctgaaa
     6301 ctattgatga tattgacaaa gcaattgaag ctgaaatttg gtatcctgaa tacaaatcat
     6361 acatttaatt ttataaagta gtaaaaggtc atttgaacat tttgtttcaa gtggcctttt
     6421 cgtatttact ttaaaattaa tcaaccaata ttcataagtg actttaaagt tatatctata
     6481 tgtaatatca gcaaatagaa aagtctagag agatttaacg aattatcttc tagacttttt
     6541 gcttgaagtg gagtaggaga gatcaatgtc ccactcactt actaattaaa tcttcaagtg
     6601 ccatttttaa attactatat ttaaattgga atcccaatgc ttgaatttta ttaggtaata
     6661 ctttttgagt atccaatact actgttgaca tttgaccaag tatgagacgc attgcaagac
     6721 ttggtgccca agtttcatga ggcttatgca tagctcttgc taaagtgtag ccaaataaat
     6781 tttgacgctc aggtataggt gcagttaaat taaacggacc actagctgac tcgttattta
     6841 ttaaaaataa aatagcttga attaaatcat tgatatgaat ccatgaatac cattgttgac
     6901 cagaacctaa tttaccacca atgtaatatt cgtatggtag tttcattgtt tgtaacgcac
     6961 cgccttcatt cgataaaatt ataccgaaac gaccgatgac aactcgtgta cctaattgtt
     7021 caaattgttg tgcgaaacgt tcccattgat acacaatatc tgataagaaa tcaaatggta
     7081 aagttttata aacttctgtg taactcataa ataaatcagg aggatagtaa ccagtggcac
     7141 tagcattaaa taaaacttta ggtgctttat tacgtgattt aaacaattca tataaagctt
     7201 gcgtagattg aattctactt agcattagcg tttgtttata ttccggtgtc catcgtttat
     7261 tcaatgtagc acctgctaag ttgatgacca catcgatttt ttgaggaact ttgtgttccc
     7321 acccagattt agcccagttg acatatgaaa ttttcttatc atttgaaatt tggtcgtgtc
     7381 gcgttaatat cgtgatatgt gaatctgatt ttttgatttc attaactaat tgagatccaa
     7441 ccataccagt cccaccagta attaagtatt gtttcattat cattcacccc atgtaatttt
     7501 gtatttagtt actatttaat aatcattatt gttgttcaaa ggttatacat tatttagaca
     7561 ataatatgtc aataactttt tttgaattgt attttaataa aaatgatata agttataata
     7621 aaggtgtacc ttcgataacg aataaacatc tcttaaaagt atgtgtaaaa cgctgcatga
     7681 tacaaacgaa ggtaaaaatt tgactccctt tagtagtgga cccgtacgtt tatcgtgcgg
     7741 gtcgttttca tttatatctt attcccgtta tacatcaatt caaagcatta attttttaaa
     7801 caaatttaag aatagatagt aatataacaa tctaaacata aaaactttta acacaacact
     7861 taaactaatg ctttaatttt caatacgtag ctataatttg ttgtaaaatc aaaaaggtta
     7921 atatgttaat tttcaaaaaa aggctcaaaa tatgtttgat ttcgttatta aatgttaaga
     7981 tatataagac tactatttct ttgtaaaaat gaatccgatt tacgagtgag taatagtgaa
     8041 tacagtttta agttgaagaa ggcaaaaaga gtaaatgttt attaatgttt gtagaaacaa
     8101 ggtaagcaaa ttagttgtga aaatgttaat agttacgtga taatttctat atttaaatta
     8161 gtttgaagtg agggagagta tgtcgaatca aaattacgac tacaataaaa atgaagatgg
     8221 aagtaagaag aaaatgagta caacagcgaa agtagttagc attgcgacgg tattgctatt
     8281 actcggagga ttagtatttg caatttttgc atatgtagat cattcgaata aagctaaaga
     8341 acgtatgttg aacgaacaaa atcaggaaca aaaagaaaag cgtcaaaaag aaaatgcaga
     8401 aaaagagaga aagaaaaagc aacaagagga aaaagagcag aatgagttag attcacaagc
     8461 aaaccaatat cagcagttgc cacagcagaa tcaatatcaa tatgtgccac ctcagcaaca
     8521 agcacctaca aagcaacgtc cagctaaaga agagaatgat gataaagcat cgaaggatga
     8581 gtcgaaagat aaggatgaca aagcatctca agataaatca gatgataatc agaagaaaac
     8641 tgatgataat aaacaaccag ctcagcctaa accacagccg caacaaccaa caccaaagcc
     8701 aaataataat caacaaaaca atcaatcaaa tcaacaagca aaaccacaag caccacaaca
     8761 aaatagccaa tcaacaacaa ataaacaaaa taatgctaat gataagtagt atttagtcaa
     8821 acaaaaatga accagtatga cagacaacac aattaattag gttgtcgcga atattggttc
     8881 ttatttttat aattgttaat taggggagag atgatactta aatagttagt tgtttatttt
     8941 gcggatagtg aaatttattt tgagtgaggt gggacagaaa tgatattttc gcaaaattta
     9001 tttcgtcgtc ccaccccaac ttgcattgtc tgtagaaatt ggaaatccaa tttctctatg
     9061 ttggggcccc gccaacttgc attgcctgta gaatttcatt tcgaaattct ctatgttggg
     9121 gccccagact ttaattgaaa aaaggttgtt acaagtgcat tttcgttcag tcaactactg
     9181 gcaatgtaac ttttcagatt ctagagtgta aatgtcaata taaaattgta catgtttaaa
     9241 caatcaaaag tgtacattat taaattatca tttccatcta gagcattgat ttatgtccta
     9301 gtctcaaata ataagcaaat gattagagag tcagtataag ggtatacatt tgactacagc
     9361 gaataaaata aaacgttttg ttgtataaca aatcgaaaac atattgcaag cgctttatca
     9421 aattatttag aaaatttaag ttttatgctt gcaatttttg aaatagaata gtactattgc
     9481 aagtgtaaag aggttaattt ttgtcccacg tgggacttaa aaaggcaacc actggttgtg
     9541 acatatcctt atttacattt ataaatataa ggaggaggta gtagtgaaag acttattgca
     9601 agcacagcaa aagcttatac cggatctcat agataaaatg tataaacgtt tttctattct
     9661 tactactatc tcaaaaaatc agcctgtcgg acgtcgaagt ttaagcgaac atatggatat
     9721 gactgaacgt gtactgcgtt ctgaaacaga tatgcttaag aaacaagatt tgataaaagt
     9781 taagcctacc ggtatggaaa ttacagctga aggtgagcaa ctgatttcgc aattgaaagg
     9841 ttactttgat atctatgcag atgataatcg tctgtcagaa ggtattaaga ataaatttca
     9901 aattaaggaa gttcatgttg ttcctggtga tgctgataat agccaatctg ttaaaacaga
     9961 attaggtaga caagcaggtc aattacttga aggcatatta caagaagatg cgatagttgc
    10021 tgtaactggc ggatccacga tggcatgtgt tagtgaagca attcatttat taccatataa
    10081 tgtattcttc gtaccagcca gaggtggact aggcgaaaat gttgtctttc aggcaaacac
    10141 aattgcagcc agtatggcac aacaagctgg cggttattat acgacgatgt atgtacctga
    10201 taatgtcagc gaaacaacat ataacacatt gttgttagag ccatcagtca taaacacttt
    10261 agacaaaatt aaacaagcaa acgttatatt acacggcatt ggtgatgcgc tgaagatggc
    10321 gcatcgacgt caatcacctg aaaaggtcat tgaacaactt caacatcatc aagctgtcgg
    10381 agaggcattt ggttattatt ttgatacaca aggtcaaatt gtccataagg ttaaaacaat
    10441 tggacttcaa ttagaagacc ttgaatcaaa agactttatt tttgcagttg caggaggcaa
    10501 atcgaaaggt gaagcaatta aagcatactt gacgattgca cccaagaata cagtgttaat
    10561 cactgatgaa gccgcagcaa agataatact tgaataagag ataaaaagtt taatactttt
    10621 taaatatcat tttaaaggag gccattataa tggcagtaaa agtagcaatt aatggttttg
    10681 gtagaattgg tcgtttagca ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag
    10741 cagtaaacga cttaacagat gacgacatgt tagcgcattt attaaaatat gacactatgc
    10801 aaggtcgttt cacaggtgaa gtagaggtag ttgatggtgg tttccgcgta aatggtaaag
    10861 aagttaaatc attcagtgaa ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg
    10921 atgtagtatt agaatgtact ggtttctaca ctgataaaga taaagcacaa gctcatattg
    10981 aagcaggcgc taaaaaagta ttaatctcag caccagctac tggtgactta aaaacaatcg
    11041 tattcaacac taaccaccaa gagttagacg gttctgaaac agttgtttca ggtgcttcat
    11101 gtactacaaa ctcattagca ccagttgcta aagttttaaa cgatgacttt ggtttagttg
    11161 aaggtttaat gactacaatt cacgcttaca caggtgatca aaatacacaa gacgcacctc
    11221 acagaaaagg tgacaaacgt cgtgctcgtg cagcggcaga aaacatcatc cctaactcaa
    11281 caggtgctgc taaagctatc ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg
    11341 gtgcacaacg tgttcctgta gctacaggtt cattaactga attaacagta gtattagaaa
    11401 aacaagacgt aacagttgaa caagttaacg aagctatgaa aaatgcttca aacgaatcat
    11461 tcggttacac tgaagacgaa atcgtttctt cagacgttgt aggtatgact tacggttcat
    11521 tattcgacgc tacacaaact cgtgtaatgt cagttggtga ccgtcaatta gttaaagttg
    11581 cagcttggta tgataacgaa atgtcatata ctgcacaatt agttcgtaca ttagcatact
    11641 tagctgaact ttctaaataa ttttagtata gtttttattc aaatacgcta gtgctcagaa
    11701 ctatttagca ttaattaaag cttatgagta agcggggagc acaaacgctt ctccgcttat
    11761 ttttatataa aatttcctaa ttacaaggag gaaacaccat ggctaaaaaa attgtttctg
    11821 atttagatct taaaggtaaa acagtcctag tacgtgctga ttttaacgta cctttaaaag
    11881 acggtgaaat tactaatgac aaccgtatcg ttcaagcttt acctacaatt caatacatca
    11941 tcgaacaagg tggtaaaatc gtactatttt cacatttagg taaagtaaaa gaagaaagtg
    12001 ataaagcaaa attaacttta cgtccagttg ctgaagactt atctaagaaa ttagataaag
    12061 aagttgtttt cgtaccagaa acacgcggcg aaaaacttga agcagctatt aaagacctta
    12121 aagaaggcga cgtattatta gttgaaaata cacgttatga agatttagac ggtaaaaaag
    12181 aatctaaaaa tgatccagat ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa
    12241 atgatgcttt tggtactgcg catcgtgagc atgcatctaa tgttggtatt tctacacatt
    12301 tagaaactgc agctggattc ttaatggata aagaaattaa gtttatcggt ggcgtagtta
    12361 acgatccaca taaaccagtt gttgctattt taggtggagc aaaagtatct gacaaaatta
    12421 atgtcatcaa aaacttagtt aacatcgctg ataaaattat catcggcgga ggtatggctt
    12481 atactttctt aaaagcgcaa ggtaaagaaa ttggtatttc attattagaa gaagataaaa
    12541 tcgacttcgc aaaagattta ttagaaaaac atggtgataa aattgtatta ccagtagaca
    12601 ctaaagttgc taaagaattt tctaatgatg ccaaaattac tgtagtacca tctgattcaa
    12661 ttccagcaga ccaagaaggt atggatattg gaccaaacac tgtaaaatta tttgcagatg
    12721 aattagaagg tgcgcacact gttgtatgga atggacctat gggtgtattc gagttcagta
    12781 actttgcaca aggtacaatt ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta
    12841 cgattatcgg tggcggtgat tcagctgcag cagcaatctc tttaggtttt gaaaatgact
    12901 tcactcatat ttcaactggt ggcggagcgt cattagagta cctagaaggt aaagaattgc
    12961 ctggtatcaa agcaatcaat aataaataat aaagtgatag tttaaagtga tgtggcatgt
    13021 ttgtttaaca ttgttacggg aaaacagtca caagatgaca tcgtgtttca tcacttttca
    13081 aaaatattta caaaacaagg agtgtcttta atgagaacac caattatagc tggtaactgg
    13141 aaaatgaaca aaacagtaca agaagcaaaa gacttcgtca atgcattgcc aacattacca
    13201 gattcaaaag aagtagaatc agtaatttgt gcaccagcaa ttcaattaga tgcattaact
    13261 actgcagtta aagaaggaaa agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc
    13321 gaagataatg gtgcgttcac aggtgaaacg tctccagttg cattagcaga tttaggcgtt
    13381 aaatacgttg ttatcggcca ttctgaacgt cgtgaattat tccacgaaac agatgaagaa
    13441 attaacaaaa aagcgcatgc tattttcaaa catggtatga caccaattat ttgtgttggt
    13501 gaaacagatg aagagcgtga aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag
    13561 aaagctgttg caggtttatc tgaagagcaa cttaaatcag ttgtaattgc ttatgaacca
    13621 atctgggcaa tcggaactgg taaatcatca acatctgaag atgcgaatga aatgtgtgca
    13681 tttgtacgtc aaactattgc tgacttatca agcaaagaag tatcagaagc aactcgtatt
    13741 caatatggtg gtagtgttaa acctaacaac attaaagaat acatggcaca aactgatatt
    13801 gatggggcat tagtaggtgg cgcatcactt aaagttgaag atttcgtaca attgttagaa
    13861 ggtgcaaaat aatcatggct aagaaaccaa ctgcgttaat tattttagat ggttttgcga
    13921 accgcgaaag cgaacatggt aatgcggtaa aattagcaaa taagcctaat tttgatcgtt
    13981 attacaacaa atatccaacg actcaaatcg aagcgagtgg cttagatgtt ggactacctg
    14041 aaggacaaat gggtaactca gaagttggtc atatgaatat cggtgcagga cgtatcgttt
    14101 atcaaagttt aactcgaatc aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg
    14161 ttttaaataa tgcaattgca cacgtgaatt cacatgattc agcgttacac atctttggtt
    14221 tattgtctga cggtggagta cacagtcatt acaaacattt atttgctttg ttagaacttg
    14281 ctaaaaaaca aggtgttgaa aaagtttacg tacacgcatt tttagatggt cgtgacgtag
    14341 atcaaaaatc cgctttgaaa tacatcgaag agactgaagc taaattcaat gaattaggta
    14401 ttggtcaatt tgcatctgtg tctggccgtt attatgcaat ggaccgtgac aaacgttggg
    14461 aacgtgaaga aaaagcttac aatgctattc gtaactttga tgccccaact tatgcaactg
    14521 ccaaagaagg tgtggaagca agttataatg aaggcttaac tgacgaattc gtagtaccat
    14581 tcatcgttga gaatcaaaat gacggtgtta atgatggaga tgcagtgatc ttctataatt
    14641 tccgacctga tagagcagca caattatcgg aaatttttgc gaacagagca ttcgaaggct
    14701 ttaaagttga acaagttaaa gatttattct atgcaacatt cactaagtat aatgacaata
    14761 tcgatgcggc tatcgtcttc gaaaaagtcg atttaaataa tacaattggt gaaattgcac
    14821 aaaataacaa tttaactcaa ttacgtattg cagaaactga aaaatatcct cacgttactt
    14881 actttatgag tggtggacgc aatgaggaat ttaaaggtga acgtcgtcgt ttaattgatt
    14941 cacctaaagt tgcaacgtat gacttgaaac cagaaatgag tgcttatgaa gttaaagatg
    15001 cattattaga agagttaaat aaaggtgact tggacttaat tattttaaac tttgctaacc
    15061 ctgatatggt tgggcatagt ggtatgcttg agccgacaat caaagcaatc gaagcggttg
    15121 acgaatgttt aggcgaagtg gttgataaga ttttagacat ggatggttat gcaattatta
    15181 ctgctgacca tggtaactct gatcaagtat tgacggatga tgatcaacca atgactacgc
    15241 atacaacgaa cccagtacca gtgattgtaa caaaagaagg cgttacactt agagaaactg
    15301 gtcgcttagg tgacttagca cctacattat tagatttatt aaatgtagaa caacctgaag
    15361 acatgacagg tgaatcttta attaaacact aatattgtaa aagatgttaa gtaaacgctt
    15421 aatgacactt attttttgaa aataatagta atatcatttt gttaaatgaa agaataaagc
    15481 tataataatt atagaataac tatttaaagg agattataaa catgccaatt attacagatg
    15541 tttacgctcg cgaagtctta gactctcgtg gtaacccaac tgttgaagta gaagtattaa
    15601 ctgaaagtgg cgcatttggt cgtgcattag taccatcagg tgcttcaact ggtgaacacg
    15661 aagctgttga attacgtgat ggagacaaat cacgttattt aggtaaaggt gttactaaag
    15721 cagttgaaaa cgttaatgaa atcatcgcac cagaaattat tgaaggtgaa ttttcagtat
    15781 tagatcaagt atctattgat aaaatgatga tcgcattaga cggtactcca aacaaaggta
    15841 aattaggtgc aaatgctatt ttaggtgtat ctatcgcagt agcacgtgca gcagctgacc
    15901 tattaggtca accactttac aaatatttag gtggatttaa tggtaagcag ttaccagtac
    15961 caatgatgaa catcgttaat ggtggttctc actcagatgc tccaattgca ttccaagaat
    16021 tcatgatttt acctgtaggt gctacaacat tcaaagaatc attacgttgg ggtactgaaa
    16081 ttttccacaa cttaaaatca attttaagta aacgtggttt agaaactgca gtaggtgacg
    16141 aaggtggttt cgctcctaag tttgaaggta ctgaagatgc agttgaaaca attatccaag
    16201 caatcgaagc agctggttac aaaccaggtg aagaagtatt cttaggattt gactgtgcat
    16261 catcagaatt ctatgaaaat ggcgtatatg actacagcaa gttcgaaggc aagcacggtg
    16321 caaaacgtac agctgcagaa caagttgact acttagaaca attagtagac aaatatccta
    16381 tcatcacaat tgaagacggt atggacgaaa acgactggga tggttggaaa caacttactg
    16441 aacgtatcgg tgaccgtgta caattagtag gtgacgattt attcgtaaca aacactgaaa
    16501 ttttagctaa aggtattgaa aacggaattg gtaactctat cttaattaaa gttaaccaaa
    16561 tcggtacatt aactgaaaca ttcgatgcaa tcgaaatggc tcaaaaagct ggttacacag
    16621 cagtagtttc tcaccgttca ggtgaaacag aagatacaac aattgctgat atcgctgttg
    16681 ctacaaacgc tggtcaaatt aaaactggtt cattatcacg tactgaccgt attgctaaat
    16741 acaatcaatt attacgtatc gaagatgaat tatttgaaac tgctaaatat gacggtatca
    16801 aatcattcta taacttagat aaataatcat tttaaacatt aagtactgcc ataattttag
    16861 ttgaggatta ttatgtcggt ataaattgaa taaagttttt gagttcacgc ttaaaaagtt
    16921 cacgcttgta tttataacct gccacagagt tgagactgtg gtaggttttt tattttgaag
    16981 tattattcat aacagactaa taatcatgag gtaactaata acacatattt aacttgtatt
    17041 cttaaactgg tataataaat ttatgttgaa atgaatattg tatgacaggg tattcacttt
    17101 tattaaaagg taaaattaaa taaaggtttt atagaacgta tttaaatata tgaggagtaa
    17161 acaaatggct gatagaacga ataaagaaat taaaacagga cgctttattg caactgcatc
    17221 aatcgtattc tcaatattgt tgattattca ttactttgtt tcgttggata atgcgactgc
    17281 caaagcatta ctcaatttaa cgaatcaaaa cacttcagat aaagcgatag attacatttt
    17341 aaacagcttt agattcactg gtataatgta tattttggct tatctagcag gcttcatcac
    17401 tttttggaat cgacatactt atgtgtggtg gtttatgttt gcagtttatg tatcaaatag
    17461 tttgtttacg ttgattaatt tatcaatcac aattcaagca ataaaagctg cacacggtgc
    17521 gtacttaaca ttgccaattt taattgttat tataggttcg gttgcattag cgatttatat
    17581 gcttgttgtt tctatcaaac gtaaaagtac atttaatcgc tagaaaattg attttaacaa
    17641 taaaaatatg atatactact tgtcgtatat aaggaacgga ggacaattta tgcatacatt
    17701 tttaatcgta ttattaatca ttgattgtat tgcattaata actgttgtac tactccaaga
    17761 aggtaaaagc agtggacttt caggtgccat cagtggtggt gctgagcagt tattcggtaa
    17821 acaaaaacaa cgtggcgtcg atttattctt aaatagatta acaattattt tatcaatatt
    17881 attttttgta cttatgattt gcataagtta tcttggtatg taaggtccgg cgatgtaaat
    17941 gtcgggcttt tttatttata attagaataa taaaggactc atgttcaatt attagaaaca
    18001 tattgtttaa ttaagtacgt ttgttaattg tgaaaaagga gcgttagatt tatgcaaata
    18061 aagttaccaa agcctttctt ttttgaggaa ggtaaacgtg ccgtgttatt actacatggt
    18121 tttacaggca attcgtctga tgttcgtcaa ttaggtcgat ttttacaaaa gaaaggttat
    18181 acatcgtatg caccacaata tgaaggtcat gcagcaccac cagaagagat acttcaatca
    18241 agtccttttg tttggtttaa agatgcctta gatggctatg actaccttgt tgaacaaggt
    18301 tatgatgaaa tcgttgttgc tggcttgtca ttaggtggcg attttgcatt aaaattaagc
    18361 ttaaatcgag atgtaaaggg tattgtaacg atgtgtgctc ctatgggtgg caaaactgaa
    18421 ggtgccattt atgaaggctt tttagaatat gcacgcaatt ttaaaaagta tgagggtaaa
    18481 gatcaagaaa cgattgataa cgaaatgcat catttcaaac caactgaaac tttaaaagaa
    18541 ttaagtgaag cattagatac gattaaagat caagttgatg aggtgtttga cccaatttta
    18601 gtgattcaag cagaaaacga caatatgatt gatccacaat ccgcaaatta tatatatgaa
    18661 catgtagact ctgacgaaaa agatatcaaa tggtatagtc agtcaggaca tgttattaca
    18721 attgataaag aaaaagaaca agttttcgat gatatttatc aatttttaga gtcattagac
    18781 tggtcagaat aaaaagagat tttaacatta gaaaggaggg gcataatgaa tttaaagcaa
    18841 tcaatagaag agattattaa tcaacctgaa tatgaaccta tgtcagtgtc agattttcaa
    18901 gatgcattag gtttaagcag tgccgactcg tttagagatt taattaaggt gcttgtggag
    18961 ttagaacaat caggattaat cgaacgtaca aaaacggaca gatatcaaaa aaagcatagt
    19021 tcaaaaggtc attcaaaatt gataaaggga acgttaagtc aaaacaaaaa aggttttgct
    19081 ttcctaagac ctgaagacaa agatatggaa gatatattta ttcccccaac aaaaattaat
    19141 cgtgccttgg atggagatac tgttattgta gaaatccatc aatcaaaagg tgagcataaa
    19201 ggtaaaattg aaggggaagt taagtcaatt gagaagcatt cagtaactca agtggttggt
    19261 acgtatagtc aggctagaca ttttggtttt gtcattccag atgataaacg aattatgcaa
    19321 gatattttca ttcctaaagg tcaaagctta ggtgcagtag atggtcacaa agtattagtt
    19381 caaattacta agtatgctga cggttcagat aatccagaag gacatatttc tgctatttta
    19441 ggacataaaa atgatcctgg cgtagatatt ttatctatta tctatcaaca tggcatagaa
    19501 attgaatttc ctgatgaagt gttacaagaa gctgaagcaa caccagacaa tatagaagat
    19561 agtgaaatta aaggacgtca tgatttacgt gatgaattga caattactat tgatggcgcg
    19621 gatgctaaag acttagatga cgcaattagt gttaaaaagt tagcgaatgg taatacgcaa
    19681 ttaactgtaa gtattgctga tgtcagctat tatgtaacag aaggttctgc attggataaa
    19741 gaggcatatg atagagctac aagtgtatat cttgttgacc gtgtaattcc aatgattcca
    19801 catcgattaa gtaatggtat ttgttcatta aatcctcacg ttgatcgttt aacgttaagc
    19861 tgtcgcatgg aaattgatgc tagtggtcgc gttgttaaac atgaaatctt tgatagtgtg
    19921 attcattctg attatcgaat gacgtatgat gcggtaaatc agattattac tgaaaaggat
    19981 cctacgattc gagagcagta taatgaaatt acgcctatgc tagatttagc acaagattta
    20041 tcaaatcgtt taattcaaat gagaaaacgc cgtggtgaaa ttgattttga tattagcgaa
    20101 gcgaaagtac tagttaacga agatggtata cctacagatg ttcaattgag acaacgtggt
    20161 gaaggcgaac gtcttattga gtcatttatg ttaattgcaa atgaaacagt tgctgaacat
    20221 tttagtaagt tagatgtacc atttatttac cgagtgcatg agcaacctaa atcagatcgc
    20281 ttaagacaat tctttgattt tattacaaac tttggcatca tgattaaggg tactggcgaa
    20341 gatattcatc caacaacact tcaaaaggtt caagaagaag tagaaggtcg acctgaacaa
    20401 atggtcattt caacaatgat gttacgttca atgcaacaag cgcattatga tgatgtgaac
    20461 ttgggacatt ttggcttatc agctgaatat tatacgcatt ttacatcacc aataagacgt
    20521 tatcctgatt taacagttca tcgtttaatc cgtaagtatt taattgagaa atcaatggat
    20581 aacaaagaag tgaagcgttg ggaagacaaa ttgcctgagt tagctgaaca tacttctaaa
    20641 cgtgaacgtc gtgctattga ggcagaacgt gatactgatg aattgaaaaa agcagaatat
    20701 atgattcaac atattggtga tgaatttgaa ggtattgtca gctcagtagc taacttcggt
    20761 atgttcattg aattgccaaa tacgatagaa ggtatggttc atattgcgaa tatgactgat
    20821 gattattacc gttttgaaga gcgtcaaatg gcattaattg gtgagcgtca agctaaagta
    20881 tttagaattg gtgacacagt taaggttaaa gtgacgcatg ttgatgtaga tgaacgatta
    20941 attgattttc aaattgtagg tatgccttta ccgaaaaatg atcgatcaca gcgcccagca
    21001 cgaggtaaga caattcaagc taaaacgcgt ggtaaatcat tagataaatc gaaatctgat
    21061 gataaaggtc ataagaaaaa aggtaggcaa cgtaaaggta aaaatgaacg taaaaatgac
    21121 aaatcaggaa acagtaaaca taagccattt tataaagata aaagtgtgaa aaagaaagca
    21181 cgtcgtaaga aaaaataagc agcaatgagg tgagtatgaa tggctaagaa gaaatcacca
    21241 ggtacattag cggaaaatcg taaagctaga catgattata atattgaaga cacgattgaa
    21301 gcgggaattg tattgcaagg cacagaaata aaatcaattc gccgaggtag tgctaacctt
    21361 aaagatagtt atgcgcaagt taaaaacggt gaaatgtatt tgaataatat gcatatagca
    21421 ccatacgaag aagggaatcg ttttaatcac gatcctcttc gttctcgaaa attattattg
    21481 cataaacgtg aaatcattaa attaggtgat caaacacgtg aaattggtta ttcgattgtg
    21541 ccgttaaagc tttatttgaa gcatggacat tgtaaagtat tgcttggtgt cgcacgaggt
    21601 aagaaaaaat atgataaacg tcaagcttta aaagaaaaag cagtcaaacg agatgttgcg
    21661 cgcgatatga aagcccgtta ttaagcgatt tggttgctta atcgggctat atttgatata
    21721 gttatatgtg cttttgtaaa ttgcaaaagt atgatttgtt tgatttatta tttcggggac
    21781 gtttctggat tcgacagggg tcccccgagc tcattaagcg tgtcggaggg ttgtcttcgt
    21841 catcaacaca cacagtttat aataactggc aaaacaaaca ataatttcgc agtagctgcc
    21901 taatcgcact ctgcatcgcc taacagcatt tcctatgtgc tgttaacgcg attcaacctt
    21961 aataggatat gctagacact gccgtttgaa gtctgtttag aagaaactta atcaagctag
    22021 catcatgttg gttgtttatc acttttcatg atgcgaaact tttcgataaa ctacacacgt
    22081 agaaagatgt gtatcaggac ctctggacgc gggttcaaat cccgccgtct ccataaaaat
    22141 atgcttggaa accttgattt aatggggttt taatctagca agtgtcaaat atgtgtcaag
    22201 aaaataattt tctgacacgt tgaccttgct cttttttatg ttcatcaagt aagtgagagt
    22261 aggtgtctaa agttatagat atattataat ggcctaatct tttgctaata tattcaatag
    22321 gtataccttt agaaagtagg aaagatgtat gcgtgtgtct taatgaataa ggtgttattt
    22381 tagtatcatt tagtcctatt tgactcttag catggttaaa tgacttttta acggcattat
    22441 gactcaattt aaacaactta ttatctgtac gttttggtaa ttttgctaat ttagctttaa
    22501 tatgttgtat atcctttttt ggtacctcca caagtctgtc cgcgttaact gtttttgttc
    22561 cacgaagatg tattgtaccc tctttttcgt ttagatcgat aggcaacata ttaattacat
    22621 cgctgtatct tgcaccagtg atagctagga tgaataaaaa aatataactc gattcgtctc
    22681 tagatttaaa gtattctatc aattgcaagt attgttctat ggtgatgaat ttagagtgtt
    22741 cgtcttttga tttttttgta ccacgaatat ctatttgata gctagggtct ttctttaaat
    22801 agccctcata tactgcatct ctgaagcatt gtgataaaca actgtttaat ttacgaaccg
    22861 tttcattagt acgacctcga ccgaattcgt tcaaaaactt ttgatactcc gaacgtttga
    22921 tgttttttat taaaaaatca ctcccgaaat attcgttaaa taattttaat gaacgttgat
    22981 accaatagaa ttgttgtgaa gcgacatgtt tcttattttt tgaatctaac caatcattgt
    23041 aatattcttc aaacttttta ttttcatcta aattatttcc atcatccaaa tctctaagca
    23101 gttgttgagc agcgttggtt gcctcagctt tagttttgaa tcctgacttt cttttctttc
    23161 ctgatttgaa agacggatgt tttacgtcgt actgccaaga tgctgttgct ttattcttcc
    23221 tttttgtaat tgtaaatgac gccattttac ttttcctcct caaaattggc aaaaaataat
    23281 aagggtaggc gagactaccc gaaattttat tgttgaatca cttcactatt ttgacgtttg
    23341 aaattgtcaa aatcattttg tgctttcttc catgaattat agtcttgtcc gtcttgtact
    23401 gcccatgaac cacctatgcc ggcagtatgt ccaccattct gacgtttgtt ttcttctgtt
    23461 gctcttttag cttcttgata agcgttataa gatgtgtcgc ttgaaaactc atcttttact
    23521 ggcgcattgt tctttttatt agaagcggga ttattttgtg tttgattttg cttaggtgcg
    23581 ttattagttt gttgatgatc gttaacattt gtgttgttct cgttgtttac ttgattattg
    23641 ttatcgtttt gattagcatt ttcttttttc gattctgctt tgtctttagt tactttcttt
    23701 ttgtttttgt tctctttctt tgtttccgtt ttcttatttt cctctttctt atcgccgtcg
    23761 ttgctaccgc atgcacttaa cactaacgta ctagctaaaa ctaaatataa taatcttttc
    23821 atgttttata cccctttatt cgctatttgt tttaataaat ctatgatttc attgttttgt
    23881 tctatgattt tgttttcgtt tttaagatgt tcgtctaaca tctctattaa gacgaagttt
    23941 tgatttatca tttcataagt aaacatttga ccagcgttat taggattaga aaacgaacta
    24001 ctgaaacgcg ttgaaaagct atctataaat tgaccaactt tattttttaa caacatatct
    24061 ttaccgctct cagacattgt atttagttcg cgtttattta aagttttttc gataattttg
    24121 tattttgttt cctgatttct ttcgatttct tctacttcaa aaggaatatt gttattaaat
    24181 ttttcgataa tatcacgttt ttcagaaatt gacatacgat caaatacttg tttttgacct
    24241 ttatttaact tccctcgaat ttttccggca gtccaagact ctttaactgt taacttatca
    24301 ttaggaactt gattcatctt ttatacgact ccttttctca tatttcttta tatttaaaaa
    24361 ctctcaacgg ctcaaatgta atcgaatact cgccatagtg agttccaata ccgtatatct
    24421 tcttatattg ttctattgcc tccaatatgt attcttcgct taattgtaga tactcagaca
    24481 actcatacaa gttacgtacg ccataattgt aagcttctac aatttcgcgt aacgggactg
    24541 ctgagataaa gccgtgtcgt cttgcgtaat tttcgaactt gcgattgttg aatttcgatt
    24601 gatctaaaat gttgccatac gtcaacttgt ggtgggcaag ttcttcatat aatacttcta
    24661 atttgttcct ttcggataag gaaggtctaa taaaaatttc tccttcttga taccaaccat
    24721 cgaatcctcg aggtactctt tgtgtttctt tcacttcaac ttcacatttc ataagcaatt
    24781 cttcgtattt tcccatgcgc caaacccctt tggtgtctta tttctttcta tctctaaccc
    24841 attgcataaa attttcgatt tcttcccatt cttcgggagt aaattcatct ttatttgcat
    24901 gaccggctat agtttcttga tgaatacttc tttcttctgt aattctcgat ttaggtacat
    24961 taaagtaatc tgctaattgt tggacttttg atattctagg atatttaagt tctttaagcc
    25021 agttagagat tgttgattga cttaccccga ttgcttcaga caattctact tgagtaatgt
    25081 tgttctcttt cataagttgc tctaagttct ctgataaaat ttttctagca ctcttatatt
    25141 ccataatttt ctcctttagt attacttaat gtaatactaa tttaccataa gtaatatcac
    25201 ttttcaatac aaaatattac ttttttgaaa taaatatcac tttaggtgtt gacatattac
    25261 tttaagtgat agtatagttg taaatgtcaa cgggaggtga tacgaaatgc cagaaaattt
    25321 taaagagttc tctgtaaagg tctggagaac taattcgaat atgacacaac aagatgtcgc
    25381 tgataaatta ggcgttacta aacaatctgt aataagatgg gaaaaagatg acgcagaatt
    25441 aaaaggctta caattgtatg ctttagccaa attattcaac acagaagttg attatataaa
    25501 ggctaaaaaa atttaacatt aatatcactt taagtgataa aggagggaac tgaaatgcaa
    25561 gaattacaaa catttaattt tgaagaatta ccagtaagga aaattgaagt ggaaggagaa
    25621 cccttctttt taggtaagga tgttgctgaa attttagggt atgcacgagc agataacgcc
    25681 atacgcaatc atgttgatag tgaagatagg ctgatgcacc aaattagtgc gtcaggtcaa
    25741 aacagaaata tgatcatcat caacgaatct ggattataca gtttaatctt tgacgcttct
    25801 aaacaaagta aaaacgaaaa cattagagaa accgctagga aattcaaacg ttgggtaact
    25861 tcggaagttt taccaacgtt aagaaaaaca ggagcgtacc aaatacctag cgacccaatg
    25921 caagcattga gattaatgtt tgaagctaca gaacaaacta aacaagaaat taaaaacgtg
    25981 aaagatgatg ttattgattt gaaagaaaat caaaaactgg atgcgggaga ttacaacttc
    26041 ttaactagaa caatcaatca aagagtagct catatacaaa gactacatgc gataacaaac
    26101 caaaaacaac gtagcgaatt attcagggat attaattcag aagtgaaaaa gatgactggt
    26161 gcgagttcaa gaacgaacgt aagacaaaaa catttcgacg atgtaattga aatgattgct
    26221 aattggttcc cgtcacaagc tactttatac agaatcaagc aaattgaaat gaaattttaa
    26281 aacgaaatat aggagaggct gaatatggaa tacatcggat atgcagacgc aaatgcgttt
    26341 gtaaaaataa gtggcatttc aaaagatgat ctagagaaaa aagtctactc gaacaaagag
    26401 tttcaaaaag aatgcatgta cagatttggt cgaggacaaa agcgttatat aaaaattgac
    26461 aaagctattc aatttatcgg taccaattta atgattaatg aatacgaatt ataggaggat
    26521 tatcaaatga gtaacattta taaaagctat ctattagcag tattatgctt cacagtctta
    26581 gcgattgtac tcatgccgtt tctgtacttc actacagcgt ggtcgattgc gggattcgca
    26641 agtatagcaa cattcatatt ttataaggaa tacttttatg aagaataaaa aaactgctac
    26701 ttgttggagc aagtaacagt atcaaacact taagaaaaaa ttcatgttca atataaaacg
    26761 aaaaacggag gaagtcaaga tgtattacga aataggcgat gtatgtcaga aggtaattaa
    26821 tgtagacgga tttgatttta aattagcagt taagaagaag gaccacagca ttctggtgaa
    26881 tatcttagat ttagaagata agtttatcga cggcataaac ataactaatg agaacgatct
    26941 atacacagca ttagacatat taaatcaatc tatttacgaa tggattgaag aaaacgcaga
    27001 tgattatgac agactaatta acttagtcat gaaatggtag gtgcgatatg aaaccacata
    27061 aatttaaacg aatggcaatt gacttaatag aacgtgtaca aagcacttct tatcaagttg
    27121 attataagta caacgttata tgggtctggc actacagcga tgactattta ggaaaagtcg
    27181 catcaataaa tatgcacaac aatgtagatg acgataacac aatattggct agatacgaga
    27241 aagctaaaaa gatgctagcg ggggaggtgt taagcgatgg ctaatctata tgagttatca
    27301 gaagcattta aagagttgtc taatcaagat gaattagacc aaacattatt aaaagacaca
    27361 ttagattcta ttcaagcaga aatgaatgtc aaagtagata acattgtcaa ttggagacgt
    27421 gaaactttag gtgacataga tgtcatagat aaagagatta aacgacttca aaatttaaaa
    27481 aaacaaaaac aaaatttaac tgatcgatta agagattact taaaagaaat gttagaaaca
    27541 caagaagtag atagttaccg cacagctact aatcatattt ttaaacgcaa aaacggagct
    27601 agtaaaaata ttatcgatga aaaacttatt ccaaaggatt attggctatc acaagccccg
    27661 aaacttaatt ctaagcaact aatcgatgat ttgaaagctg ggaaagatat tcctggcgtt
    27721 gaattaaagg taacagaaag tctggtgatt aagtgatgaa taaatcagaa acagttgttg
    27781 aaataaataa agctatggtt gcatttcgca aggaagtaaa acaaccgctc aaagataaaa
    27841 acaatccatt ttttaaatca aaatacgtac ctcttgagaa cgttgtagaa gccattgacg
    27901 aggccgcaac acctcatgga ctgtcttata ctcaatgggc tttgaacgat gtagacgggc
    27961 gcgtaggagt cgctacaatg cttatgcatg aaagcggtga atatatcgag tatgatcctg
    28021 tatttatgaa tgcagaaaag aatacgccac aaggagcagg ctcgttaatc agctacctta
    28081 aacgttattc gctatctgcg attttcggta ttactagtga ccaagacgat gatggaaatg
    28141 aagcaagtgg aaaaaataat aatccaaaac aacaaactag aacgcaatgg gcaagtagcg
    28201 aaactataag tgttttaagg aaagaagtta tagatttcac taacttgatt aagggcacag
    28261 ataaagaagc gccgcaaaat atagtagaac aaaaatttga cataaacaac tataaattaa
    28321 cagaaaaaca agcagcagaa gctattcaaa aaatacgaaa caacgcaaaa acaattactg
    28381 gaggaaaaca ataatgttaa acagaacagt attagtagga cgcttaacaa aagatccaga
    28441 atatagaaca acgccaaatg gtgtgagtgt taccactttc actatcgcag ttaacagaac
    28501 atttactaac gctcaaggag aacgtgaggc agactttatt aactgtgtaa cttttagaaa
    28561 acaagcagaa aatgtaaata attatttatc caaagggtca ttggctggcg ttgatggacg
    28621 tttacaatca cgcagttatg aaaacaaaga cgggcaacgt gtatttgtca cagaagtagt
    28681 agcggacagt gttcaattct tagaaccgaa gaataacaac caacaaccaa acaacaatta
    28741 tcatcaacaa agacaaactc aaactggtaa taatcctttt gataatacca ctgcgattat
    28801 tgatgatgac ttaccgttct gattggaatg attagatgcc aataattact agttatatca
    28861 ctcaagatga cggtacaaca acagttgtca tctcgggtgt tgaattaggt aataaagaaa
    28921 cattactact tgataacggg tttgatgtgg aagtcgatgt gagcgtcata gatccgtttc
    28981 gaattaccgg caagcaacga cgaaaaatat tcgcgcttgt caaagacata gaagaatata
    29041 caggtcaacc aatggactat atgcgacata tgttcatcga gtatgtaagg acttactacg
    29101 gctatgatga acgtatttca ctaagtaatt gtacgagaac acaagcaagt caaatcattg
    29161 aagcaacgct tgactggacg ttctacaatg acataccact tagctacaaa acgagtaatc
    29221 tactgaaacg agataaatca ttcttatact ggtcaactgt taaccgcaac tgtgtaatat
    29281 gcggaaagcc tcacgctgac ttagcacatt acgaagcagt cggcagaggt atgaacagaa
    29341 acaagatgaa tcactacgac aaacatgtgt tagcactgtg tagacaacat cataatgaac
    29401 agcacgcaat tggtgttaag tcgtttgatg ataaatatca attgcatgac tcgtggataa
    29461 aagttgatga gaggctcaat aaaatgttga aaggagagaa aaatgaataa gttac
//
LOCUS       DAPIDM010000019        28557 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000019 DAPIDM010000000
VERSION     DAPIDM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.019"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..151)
                     /locus_tag="RJE42_000750"
     CDS             complement(<1..151)
                     /locus_tag="RJE42_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1806 family protein"
                     /protein_id="HDV5876196.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRN"
     gene            428..1186
                     /gene="nagB"
                     /locus_tag="RJE42_000751"
     CDS             428..1186
                     /gene="nagB"
                     /locus_tag="RJE42_000751"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV5876197.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDLNAEASTYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFENEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            1263..1895
                     /gene="hxlA"
                     /locus_tag="RJE42_000752"
     CDS             1263..1895
                     /gene="hxlA"
                     /locus_tag="RJE42_000752"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDV5876198.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            1897..2445
                     /gene="hxlB"
                     /locus_tag="RJE42_000753"
     CDS             1897..2445
                     /gene="hxlB"
                     /locus_tag="RJE42_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDV5876199.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            2558..3205
                     /locus_tag="RJE42_000754"
     CDS             2558..3205
                     /locus_tag="RJE42_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5876200.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     KQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            3708..5108
                     /locus_tag="RJE42_000755"
     CDS             3708..5108
                     /locus_tag="RJE42_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876201.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVESADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIALLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVAKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKD"
     gene            5653..7029
                     /locus_tag="RJE42_000756"
     CDS             5653..7029
                     /locus_tag="RJE42_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDV5876202.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITCAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     EDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLTSFEVNEM
                     LMLKNENAIVAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKLSSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTKNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPANVEHLLTQSSSIDEAIIIGIPNK
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            7031..8170
                     /locus_tag="RJE42_000757"
     CDS             7031..8170
                     /locus_tag="RJE42_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDV5876203.1"
                     /translation="MNQAVIVATKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKSLLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGGIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDKAYELGFNHGLLFKDGVTVGVDSN
                     LPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVIACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            8145..8510
                     /locus_tag="RJE42_000758"
     CDS             8145..8510
                     /locus_tag="RJE42_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDV5876204.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WSKFDLFKKYANSELILIKSAINQTQKIEVDTIYVGHLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            8513..8782
                     /locus_tag="RJE42_000759"
     CDS             8513..8782
                     /locus_tag="RJE42_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876205.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLEAD
                     QCQINYVKPILINENIEFIEQHEQEIIAINDDGEIKIKISLSTKK"
     gene            complement(8983..9150)
                     /locus_tag="RJE42_000760"
     CDS             complement(8983..9150)
                     /locus_tag="RJE42_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDV5876206.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(9684..10514)
                     /gene="thiD"
                     /locus_tag="RJE42_000761"
     CDS             complement(9684..10514)
                     /gene="thiD"
                     /locus_tag="RJE42_000761"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV5876207.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            10698..11354
                     /locus_tag="RJE42_000762"
     CDS             10698..11354
                     /locus_tag="RJE42_000762"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV5876208.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANTYLESVGKSPINWCESEA
                     "
     gene            11355..11735
                     /locus_tag="RJE42_000763"
     CDS             11355..11735
                     /locus_tag="RJE42_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDV5876209.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTSHIGEQQMNHRIANHNQMPHSQITQSTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            11868..12236
                     /locus_tag="RJE42_000764"
     CDS             11868..12236
                     /locus_tag="RJE42_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV5876210.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            12357..13841
                     /locus_tag="RJE42_000765"
     CDS             12357..13841
                     /locus_tag="RJE42_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDV5876211.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVSVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(13983..14435)
                     /locus_tag="RJE42_000766"
     CDS             complement(13983..14435)
                     /locus_tag="RJE42_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDV5876212.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(14448..15212)
                     /locus_tag="RJE42_000767"
     CDS             complement(14448..15212)
                     /locus_tag="RJE42_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV5876213.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(15761..16513)
                     /locus_tag="RJE42_000768"
     CDS             complement(15761..16513)
                     /locus_tag="RJE42_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDV5876214.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            16681..17667
                     /gene="pta"
                     /locus_tag="RJE42_000769"
     CDS             16681..17667
                     /gene="pta"
                     /locus_tag="RJE42_000769"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV5876215.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIVQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            17670..18506
                     /locus_tag="RJE42_000770"
     CDS             17670..18506
                     /locus_tag="RJE42_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDV5876216.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            19093..20013
                     /gene="mvk"
                     /locus_tag="RJE42_000771"
     CDS             19093..20013
                     /gene="mvk"
                     /locus_tag="RJE42_000771"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDV5876217.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            20018..21001
                     /gene="mvaD"
                     /locus_tag="RJE42_000772"
     CDS             20018..21001
                     /gene="mvaD"
                     /locus_tag="RJE42_000772"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDV5876218.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFIVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            21014..22090
                     /locus_tag="RJE42_000773"
     CDS             21014..22090
                     /locus_tag="RJE42_000773"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDV5876219.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            22267..22608
                     /locus_tag="RJE42_000774"
     CDS             22267..22608
                     /locus_tag="RJE42_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDV5876220.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(22660..23982)
                     /locus_tag="RJE42_000775"
     CDS             complement(22660..23982)
                     /locus_tag="RJE42_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDV5876221.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDDHHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAQLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAVAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(23969..24409)
                     /locus_tag="RJE42_000776"
     CDS             complement(23969..24409)
                     /locus_tag="RJE42_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDV5876222.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYKEKTIKDVIEDIQKEDLCHENI"
     gene            24467..24568
                     /locus_tag="RJE42_000777"
     CDS             24467..24568
                     /locus_tag="RJE42_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876223.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            25034..26440
                     /locus_tag="RJE42_000778"
     CDS             25034..26440
                     /locus_tag="RJE42_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5876224.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDMANDDKTVGEALSHNALTTGIGTGAGIATSATIVALASNPVGWAA
                     LGGLAVGIIFSNLTDLAYQNNFLGIKDKTDWVGHKIDDGIDAVKKSTEKAVDSFENAV
                     GEATKGISSHINPMKWSW"
     gene            26444..27061
                     /locus_tag="RJE42_000779"
     CDS             26444..27061
                     /locus_tag="RJE42_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDV5876225.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVISTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKTFWPVVGGTILLGVTSRKYIHLLNIQLEKILVII
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKIEDKKIILVPTLKNAVLILYSYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGIGLMLFFLMNITLIVNKKAKVIKR"
     gene            27136..27767
                     /locus_tag="RJE42_000780"
                     /pseudo
     CDS             27136..27767
                     /locus_tag="RJE42_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
     gene            28003..>28557
                     /locus_tag="RJE42_000781"
     CDS             28003..>28557
                     /locus_tag="RJE42_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDV5876226.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWF
                     IPKRYVKISREEFENLSIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIILIPTLKNFCFTLFGYVFLIFWT
                     VVFSYALLSMSYQNIIVYFAWITAI"
BASE COUNT         9981 a         4167 c         5237 g         9172 t
ORIGIN      
        1 tgtttcttaa aaaagtacca gcattaaaaa cacgttgatc gaaatggttc gcataggccc
       61 catttgtcgt ttcaacatgc agatacacag gtttatgttc aaaagaagca agtaaatcta
      121 taacttcttg ttctttaatt ggttccaaca cgttcactcc ttacactatc aatgtgttta
      181 tctttctatt ttactaaaaa ctattcaata attgtatacg attgctcaat tatttataaa
      241 ttaattttca tgaagggtaa ttactcagga ttacgtaatc atacagcatt agttttttac
      301 ttttaaaaat caaaaaaaat gttggaattt aaaaagtgtt aaacattaaa aatgatgcta
      361 tattaatggt gtatgaatga attcataagt ttttaaaatg tattaaattt gtggaggcat
      421 gtaaacaatg aaagtattaa acttaggatc gaaaaaacaa gcatcattct atgttgcatg
      481 tgagttatat aaagagatgg catttaatca gcactgtaaa ctaggtttag caactggtgg
      541 tacaatgaca gatttgtatg aacaacttgt taagttatta aataaaaatc agttaaacgt
      601 agataatgta tccacgttta atttagacga atatgtaggt ttaaccgcat cacatccgca
      661 aagttatcac tattatatgg atgacatgct tttcaaacaa tatccttatt ttaatagaaa
      721 gaacattcat attccgaatg gagatgccga tgatttgaat gcggaagcgt caacttataa
      781 tgacgtttta gaacaacaag gtcaacgtga tattcaaatt ttaggtattg gtgaaaatgg
      841 tcatattgga tttaatgaac ctggtacgcc gtttgatagc gttactcata tcgttgattt
      901 gactgaaagt actattaagg ctaatagtcg atattttgaa aacgaagatg atgttccaaa
      961 gcaagccatt tcgatgggac tagctaatat tcttcaagct aaacgtatca ttttactcgc
     1021 atttggtgaa aagaaacgtg ctgctattac acatttatta aatcaggaaa tttctgttga
     1081 tgttccagcc acattacttc acaaacaccc gaatgttgag atatatttag acgacgaagc
     1141 ttgcccgaaa aatgttgcga aaattcatgt cgatgaaatg gattgattgc aatgtttaat
     1201 taagaaatgc ctcgggaaag gttccaatag aaagataaaa agcattggaa ggatgatttt
     1261 tagtggaatt acaattagca attgatttat taaacaaaga agacgcggct gagttagcaa
     1321 ataaagtaaa agattatgta gatatcgtag aaatcggtac gccaatcatt tacaacgaag
     1381 gtttaccagc agttaaacat atggcagaca acattagtaa tgtaaaagta ttagcagaca
     1441 tgaaaattat ggatgcagct gattatgaag ttagccaagc aattaaattt ggcgcggatg
     1501 taattacaat actaggtgtt gcagaagatg catcaattaa agcagctatt gaagaagctc
     1561 ataaaaataa taaacaatta ctagttgata tgattgctgt tcaagattta gaaaaacgtg
     1621 caaaagaact agatgaaatg ggtgcggatt atattgcagt acacactggt tatgatttac
     1681 aagcagaagg gcaatcacca ttagaaagtt taagaaccgt taaatctgtt attaaaaatt
     1741 ctaaagttgc agtagcaggt ggaattaaac cagatacaat taaagaaatt gtcgctgaaa
     1801 gtcctgatct tgttattgtt ggtggcggaa tcgcaaatgc agatgatcca gtagaagctg
     1861 caaaacaatg tcgcgctgca atcgaaggta agtaatatgg ctaaatttag tgactatcaa
     1921 ttaattctag atgaattaaa gatgactttg tcacatgttg aagcggatga gttttcaacg
     1981 tttgcgtcca aaatactaca tgctgaacat atatttgtag ctggcaaagg acgttcagga
     2041 ttcgtggcga atagttttgc aatgcgctta aatcagctcg gcaaacaggc acatgttgtt
     2101 ggagaatcaa cgacacctgc gattaagtcg aatgatgtat ttgtaattat ctctggttca
     2161 ggttccacgg aacatttaag attattagca gacaaagcaa aatcagtagg tgctgacatc
     2221 gtattaatta ctacaaataa agattctgca ataggcaatc tagctgggac gaacatcgtt
     2281 ttgcctgcag gtacaaaata tgatgaacaa ggttcggcac aaccattagg aagtttgttt
     2341 gaacaagcat cacaattatt tttagatagt gttgtaatgg gattgatgac tgaaatgaat
     2401 gttacggaac aaacaatgca acaaaatcat gctaatttag aataaataaa agatagtcga
     2461 taatattatg ccattgagat aatattatcg attatttttt tatttaaata ttaaattata
     2521 gtataatgtc attaacaaac gaacgggagt gttaatagtg aagtttgaca attatatttt
     2581 tgatttcgat ggtacattag cagacacgaa aaaatgtggt gaagtagcta cacaaagtgc
     2641 atttaaagca tgtggcttaa cggaaccatc atctaaagaa ataacgcatt atatgggaat
     2701 acctattgaa gaatcatttt taaaattagc agaccgacca ttagatgaag cagcattagc
     2761 aaagttaatc gatacattta gacatacata tcaatctatt gaaaaggact atatttatga
     2821 atttgcgggt ataactgaag ccattacaag tttgtataac caagggaaaa aacttttcgt
     2881 ggtgtctagt aagaagagtg atgtattaga aagaaattta tcggctatag ggttaaatca
     2941 tttaatcact gaagctgttg gatccgatca agtaagtgca tataaaccaa accctgaagg
     3001 catacataca attgtgcaac gctacaattt aaatagcaaa caaacggtgt atattgggga
     3061 ttcaacgttt gatgttgaga tggcgcaaca tgctggtgta ccatctgcag cagtaacatg
     3121 gggtgcacat gatgcaagat cattacttca ctctaatccg gattttatta ttaatgatcc
     3181 atcagaaatt aataccgtat tataaaattt gttaaaacag agaataccat ggttaaaatc
     3241 catatttata aatattagat tatacataga aatatttcgc tttagattag gaatttaaaa
     3301 taaatattta ttaataatcg tgtatgttta aatagaaaag tctgcgtaat taattatttg
     3361 atattaatac aaataactcc gactgatgac atattacttg cagtcaattg tatatcttac
     3421 tttaaatacg atgtcgaaaa tggcgatagc atatgactta tatgaagttg tatgctatcg
     3481 ctatttttag ttataattcc aaaaagttaa tcgttcgatg atttaagaat tattattgtt
     3541 taattcaaat gtacgagggt ataaaatcat tgaatttaat tcgataaagt gaaatttttg
     3601 aataaacata cttttgtatt tatataaaag tttaaattct tataaatttg acaaaactaa
     3661 ttaactccgt ataattatga aacatacaag agggagtgta tgaattcatg gattttaata
     3721 aagagaatat taacatggtg gatgcaaaga aagctaaaaa gactgttgtt gcaaccggta
     3781 tcggtaatgc aatggaatgg tttgattttg gtgtctatgc atatacaact gcgtacattg
     3841 gagcgaactt cttctctcca gtagagagtg cagacattcg acaaatgttg actttcgcag
     3901 cattagccat tgcgttttta ttaaggccaa ttggtggtgt cgtatttggt attattggtg
     3961 acaaatatgg acgtaaagtt gtattaacat caacaattat tttaatggca ttttcaacat
     4021 taaccattgg attattacca agttatgatc aaattggact ttgggcacca atactattat
     4081 tacttgcacg agtactacaa ggattttcaa caggtggaga gtatgcgggg gcaatgacat
     4141 atgtagccga atcatctcca gataagcgtc gtaactcatt aggtagtgga ttagaaatag
     4201 ggacattatc aggttatata gctgcgtcaa ttatgattgc cttactcact ttcttcttaa
     4261 ctgatgaaca aatggcatca tttggttgga gaatcccatt cttacttggt ttattcctag
     4321 gattattcgg cttatattta cgtcgtaagc ttgaagaatc accagttttc gaaaatgatg
     4381 ttgcaacaca accagaacga gacaacatta actttttaca aatcatcaga ttttattaca
     4441 aagatatatt tgtgtgtttt gtagctgttg tattcttcaa tgttacgaac tatatggtaa
     4501 ccgcatactt accaacttac ctagaacaag ttattaaact agatgcaacg acaactagtg
     4561 tattaattac ttgtgttatg gcaataatga ttccattagc attaatgttt ggtaagttag
     4621 cggataaaat aggtgaaaag aaagtatttc taatcggtac tggcgggtta acattattta
     4681 gtatcatcgc atttatgtta ttacattcac aatcatttgt tgtgatagtc atcggtatat
     4741 ttatattagg atttttctta tcaacttacg aagcaacaat gccagggtcg ttaccaacga
     4801 tgttttacag tcatataaga tatcgaactt tatcagtaac atttaatatc tctgtttcga
     4861 tatttggtgg tacgacgcca ttagttgcaa catggttagt cgcgaaaact ggtgatccat
     4921 tagcacctgc gtattattta acagcaatca gtgttattgg gtttttagtg atcacattct
     4981 tacatttaag tacagcagga aaatctctca aaggttcata tccaaatgta gataacgagc
     5041 aagatagagc ttattatgca gaacacccaa aagaagcatt atggtgggtt aaagaacgta
     5101 aagattagag atttcagtaa ataagtaata atcaatcgta taaaagcact ttaaagctag
     5161 taaacgctac taactttaaa gtgcttttta aattagggaa tgtaattagc cgaaataaag
     5221 tttttgtata tacataaacc cccactgcaa tgattatcgc aatgggggaa agaggggact
     5281 taaagcatat gtttagcttt gaatacttaa aattctcttg ctattgaaat gttaggatgt
     5341 aaatatgtct tagagtattt tgtccaacgc aattaatatt gagactctaa ccttcaatat
     5401 tattatagag aacacaaact taaatagatt gggtgactta tttgtgtcag ttattgcgat
     5461 tgcgataact tcttttctct atatacatat agtaacgtct tatctaataa aaaacatggt
     5521 actacagtat caaatttatc tagggcttaa gtttgatttt tataataggc aggtttacct
     5581 gataaaaata ctcattcatt atataatgtt aacaagatgt attttaaagt ttacatttag
     5641 tgagggatat tgatgaacgt aattttagaa cagttgaaaa cacatactca aaataaacct
     5701 aatgacatag cattacatat cgatgatgaa acaattacat atagtcaact aaatgcccgc
     5761 atcacttgcg cagttgaatc tttgcagaaa tattcactta accctgtcgt tgctattaat
     5821 atgaaatcac cggtgcaaag tattatttgt tatttagctt tgcatcgttt acataaagtg
     5881 cctatgatga tggaaggtaa atggcaaagt actatacatc gtcaattgat tgaaaaatat
     5941 ggtattaaag atgtaattga agatacaggt ctcatgcaga gtatagactc accgatgttt
     6001 attgattcaa cgcaattaca gcactacccc aatttattac atattggttt tacttcaggg
     6061 acaactggac tgccaaaagc atattatcgt gatgaagatt catggttgac ttcttttgaa
     6121 gttaatgaaa tgttgatgtt aaaaaatgaa aatgccatag tagcccccgg accactatcg
     6181 cactcgttaa cattatatgc gttattgttt gctttaagtt ccggtcgtac ttttatagga
     6241 cagaccactt ttcatcctga aaagttactt aatcaatgtc gtaaattatc atcatacaaa
     6301 gttgctatgt ttcttgttcc aacgatgatt aaatcattat tgttagttta caacaatgaa
     6361 catacaatcc aatcattttt tagcagtgga gataagttgc attcttctat ttttaaaaag
     6421 ataaaaaatc aagcaaatga cataaatttg attgaatttt ttggtacatc ggaaaccagt
     6481 tttatcagct ataacttgaa tcagcaagca ccagttgaat cagtaggtgt gctatttcca
     6541 aatgtggaat tgaaaacaaa gaatcacgat cacaatggta taggaactat ttgtataaaa
     6601 agtaatatga tgtttagtgg ctatgtaagc gaacaatgta taaataatga tgaatggttt
     6661 gttactaatg ataatggcta tgtaaaagag cagtatttat atttaacggg ccgacaacat
     6721 gatatgttaa tcattggtgg tcaaaatata tatccagcaa atgttgaaca ccttttaacg
     6781 caatcgtcga gcattgatga agcaattata attggtattc caaataagcg ttttggtcaa
     6841 ataggcgtat tgctttattc tggtgatgtg acacttacac ataaaaatgt aaaacaattt
     6901 ttaaaaaaga aagtgaaacg ctatgaaatt ccatcgatga ttcatcatgt agaaaagatg
     6961 tattacactg caagtggtaa aattgctaga gaaaaaatga tgtcgatgta tttgagaggt
     7021 gaattataat atgaatcaag cagtcatagt tgcaactaaa cgaactgcat ttgggaaata
     7081 tggtggcact ttaaaacatt tagagccaga acaattgctc aaacctttat tccaacattt
     7141 taaagagaag tatccagagg taatatctaa aatagatgat gtagttttag gtaatgttgt
     7201 tgggaatggt ggcaatattg caagaaaatc attgcttgaa gcggggctta aagattcaat
     7261 acctggcgtc acaatcgatc ggcaatgtgg gtctggactt gaaagtgttc aatatgcatg
     7321 tcgcatgatt caagccgggg ctggaaaggt atatattgca ggcggtgttg aaagtacaag
     7381 tcgagcacct tggaaaatca aacgaccgca ttctgtgtac gaaacagcat tacctgagtt
     7441 ttatgagcgt gcatcatttg cacctgaaat gagcgaccca tcaatgattc aaggtgctga
     7501 aaatgtagcc aagatgtatg atgtttcaag agaattacaa gatgaatttg cttatcgaag
     7561 tcatcaattg acagcggaaa atgtaaagaa tggaaatatt tctcaggaaa tattacctat
     7621 aaccgttaaa ggaggaatat tcaacactga tgaaagtcta aaatcacata ttccgaaaga
     7681 taactttggc cgatttaagc ccgtaatcaa aggcgggacc gttaccgctg cgaatagttg
     7741 tatgaaaaat gatggtgcag ttttattgct tattatggaa aaagataagg catacgaatt
     7801 aggtttcaat catggtttat tatttaaaga tggtgttacg gtaggtgttg attctaattt
     7861 acctggcatt ggtccagtac cagccatttc caacttacta aaaagaaatc aattaacgat
     7921 agaaaatatt gaagtcattg aaattaacga agcgttcagt gcacaggtga ttgcctgcca
     7981 acaagcttta aatatttcaa atacgcaatt aaatatatgg ggtggtgcat tagcatcagg
     8041 tcatccatac ggtgcaagcg gtgcccaatt agtgacgcga ttattttata tgtttgacaa
     8101 agagactatg attgcatcta tggggattgg gggaggtcta ggaaatgcag cattatttac
     8161 tcgattctaa ccagcgatta aatgtgtcat tttctaagga tagtgtggct gcatattatc
     8221 agtgttttaa ccaaccttat agaaaagaag taccaccatt aatgtgtgcg tcattatggt
     8281 caaaatttga tttatttaaa aaatatgcaa atagcgaact gattttaata aaatcagcaa
     8341 ttaatcaaac gcaaaagata gaagtagaca caatatatgt aggacattta gaagatattg
     8401 aatgccgaca gattcgcaat atcacacgtt atacaatggc tttaacatta actaaaaatg
     8461 atcaacatgt cataacggtt acacaaactt ttattaaggc gatgaagtag agatgaagtt
     8521 taatgagata tggataaatg aatatttggc gctcgtaaat gatgataatc caatacataa
     8581 tgagattgtg ccaggacaat tagtgagtca aatgatgctg atggctatgt cattagaggc
     8641 ggaccagtgt caaattaatt acgttaaacc tattttaata aatgaaaata tcgaattcat
     8701 tgaacaacac gagcaagaaa ttatagcaat taatgacgat ggagagatta aaataaagat
     8761 ttctttgagc acaaaaaaat aaccgatatt agctgcatga acgcatatta attaggagat
     8821 gaaaggacag ctaatatcag ttatgtattg ttattattat tggaaacaga gataaatata
     8881 ggttacgctt ctttctttgc acggggatgc attaatctaa aataataata acaactatat
     8941 caatgtttaa taaattctgg attattggaa cgattagtca atttaactaa ctttcatatg
     9001 atctatatcg tcttgtaata aagagagcaa tttgaatatt tcagtatcac taaatgaatc
     9061 gtcacattta attgaaacat gctgaaacgt tttggttata atttcataaa ctggtgcgcc
     9121 ttcatggtga tactgtcgat aaataatcat aacctatatt acctcctttg ctactctatg
     9181 gttatattat aaataacatt tttatgtgtg acatcaacct taagtatcaa ctttttatca
     9241 gacatagaac gtataattta ctaagactat ttatgtatca tgttttaaaa ggaatatatt
     9301 tttataaagt cgttgagaag ttatttttaa tttcaaaaaa atagcagaat tagtagagcg
     9361 gaataaaacg ttatgtttaa acatgggcat aataaagttc cgaaaagatt tggattgata
     9421 ggataactga ttcgtgacac tgtaactgaa tattatatac atatgttgaa ataaaactac
     9481 ctataacgat gacttacaat acttttttgc ccaatttaac tgtggagatc aaacaagtat
     9541 ttaataacta aaataaccac accgaaagtg actttattgt gtactgagcc ccaaagttag
     9601 tctttgaaag ctaactttac tggcgcacag caatgacaat caaatcataa ggtgtggtta
     9661 atctattttt tatgaacgaa gcattaaacc tctgttactt caacatcgat atgttcaatg
     9721 cggttgtatg caccgtgatc cacaggacca acaaaatcat tcattttcca accgttttta
     9781 atagcagaag cgacgaaagc tttcgcgcta atcacagctt ctttcggtga cttaccgtta
     9841 gctaaatatg cagttgttgc cgcagcaaat gtacaaccag caccatggtt ataactttgt
     9901 tggaacatgt ctgtagttag ttgataaaat gtttgaccat catagtataa gtcatacgat
     9961 ttatcttgat ctaaagcttt gccaccttta atgatgacat gctgcgcgcc tttatcaaag
    10021 ataattgttg cagccttttt catatcttca attgaattta atttacctaa tcctgataat
    10081 tgacctgctt caaataagtt tggtgtcact accgttgctt taggtagtaa atatttaatc
    10141 atcgcctcag tatttccagg gttaagcact tcatcttcgc ctttacaaac catgacagga
    10201 tctactacaa agtattgtgc attagatgcc tcatatactt ctccagcacg tttgattatc
    10261 tcctcagtac ctaacatacc tgttttaata gcatcaggtc cgattgataa agccgtttca
    10321 agttgctttt caaatacgtc cataggtaat ggtgtgacat cgtgtgacca tgtatcttta
    10381 tccatagtaa cgatggcagt taaagcgacc atgccatacg tatctaattc ttggaacgtt
    10441 ttcaaatctg cttgcatacc tgcgccagca cttgtgtcag aaccggcaat tgttaaaact
    10501 ttctttaaag ccattgagct tcactcctac ataataatat tgtattcatc atatcatttt
    10561 taacctaatt gaaaaatatt aagcattcaa tatttgatga ttgttgaaat gaatcattca
    10621 tactattgta acttttgaaa atgtcattca ctttagataa gtgtgatatg ttaaaatatg
    10681 tcctgaggtg agattgaatg gaatggtcgc aaatttttca tgacataaca acgaaacatg
    10741 actttaaagc tatgcatgat tttttagaaa aagaatattc gactgcaatc gtataccctg
    10801 atagggaaaa tatatatcaa gcgtttgatt taacaccgtt tgaaaatatc aaagttgtta
    10861 tattaggaca agacccgtat catggtccaa accaagcaca tggattagca ttttcagtgc
    10921 aacctaacgc aaaattccct ccatctttac gaaatatgta taaagaatta gcagatgata
    10981 ttggatgtgt tagacaaaca ccacatttac aagattgggc aagagaaggc gtcttgttat
    11041 tgaatacagt tttaaccgta agacagggtg aagcaaattc tcatcgtgat attggttggg
    11101 aaacatttac tgatgaaatt attaaagcag tgtctgatta taaagaacat gttgtcttta
    11161 ttttgtgggg gaaacctgca cagcaaaaaa taaagcttat cgatacatct aaacattgta
    11221 ttataaaatc agtgcatcct agtccactgt ctgcatatag aggattcttt ggatcaaaac
    11281 cgtattccaa agcgaatacc tatttagagt cagtaggaaa atcaccaatt aattggtgtg
    11341 aaagtgaggc gtagatggtg aatagagaaa ctttaatagc acgaattgag caagaattag
    11401 tacaagcaga gcaggcgcag catgaccatg actttgaaaa acatatgtat gccatacata
    11461 tattaacatc tttatatgct tcaacatcaa atacatcaca tattggtgaa caacaaatga
    11521 atcatcgtat tgctaaccat aatcaaatgc cacattcaca aattacgcag tcaacacatc
    11581 aagtgacagc tgctgaaatt gaagcgatgg gtggtaaagt aaatacgcat tcagcacatc
    11641 atcataataa gtcatattca caaccttcaa accaacaaca aagattagcg acagatgatg
    11701 acattggcaa tggtgaatcc atatttgatt tttaaaaagc aacaatgaaa cataattact
    11761 taatagctta ttaagtatgt aggttaataa tcaagacgta tatactttta ttcgagtgtt
    11821 cggatttaaa catttattaa tactgaatta tataaggaga ggtagcaatg aaattattta
    11881 ttattttagg tgcattaaac gcgatgatgg ctgtcggaac aggtgcattt ggtgcgcatg
    11941 gtttacaagg aaaaataagt gatcactatt tatcagtatg ggaaaaagca acgacgtatc
    12001 aaatgtacca tggcttagca ttattaatta taggtgtaat tagtggtaca acttcaatca
    12061 atgttaactg ggctggctgg ttaatatttg ctggtattat tttctttagt ggatcattat
    12121 atattttagt attaactcaa attaaagttt taggtgcgat tacaccaatt ggtggcgtat
    12181 tgttcatcat tggatggata atgttaatca ttgcgacatt caaatttgct ggttaaattt
    12241 taaaacttta gattacctat gtaactaaac attaaatttt taataaaaat aatcaagaaa
    12301 aagagttaca aactcatctt ttgggtatag aataccttcg aggtgagttt ttatttatgg
    12361 aaaaaaagaa taagcaaata gatagaggcg atttaaaaca aaacctatct gaaaagtttg
    12421 tatgggcgat tgcatatggt tcatgtatcg gatggggcgc attcatctta ccaggagact
    12481 ggattaagca gtcaggtccg attgcagcat caattgggat tgttattggt gcattattaa
    12541 tgatattaat tgcggttagt tatggcgcat tagtagagag atttccagta tcagggggcg
    12601 cgtttgcctt tagtttctta agtttcggca gatatgtgag tttcttctca tcatggtttt
    12661 taacttttgg ttatgtctgt gttgttgctt taaatgcgac cgcattcagt ttactagtta
    12721 aattcttatt gccagatgtc ttaaataatg ggaaactata caccattgcg ggctgggacg
    12781 tttatattac ggaaatcatt attgcgaccg tattactact tgtattcatg ctagtaacga
    12841 ttcgtggcgc aagtgtatct ggatcattac aatattattt ctgtgtggcg atggtaatcg
    12901 ttgtattatt gatgttcttt ggttcattct ttggtaataa ttttgcactt gaaaatttac
    12961 aaccgttagc tgaacctagc aaaggatggt tagtgtctat tgtggttatt gtatccgtgg
    13021 caccatgggc atatgttgga tttgataata ttccacaaac agcagaagag tttaactttg
    13081 caccaaacaa gacatttaag cttatcgtgt acagtttatt agcagcatca ttaacttatg
    13141 ttgtcatgat tttatacact ggttggttat caacaagtca tcaaagttta aatggacaat
    13201 tgtggttaac aggtgctgtt acacaaacag catttggtta tattggatta ggtgtattag
    13261 caattgcaat tatgatgggt atatttactg gtttaaatgg attcttgatg agttcaagtc
    13321 gcttgttatt ttctatggga cgttcaggta ttatgccaac aatgtttagt aaattacata
    13381 gtaaatacaa aacaccatat gtcgcaatca tattcttagt aagtgtgtct ttaattgcac
    13441 cttggctagg aagaacggca ttgacttgga tcgtagatat gtcatctaca ggtgtatcca
    13501 ttgcctactt tattacatgt ttgtctgctg cgaaattatt cagttataac aaacaaagta
    13561 atacgtatgc accggtttac aaaacgtttg ctattattgg ctcatttgta tcattcattt
    13621 tcttagcgtt gttattagtg ccaggttctc ctgcagcact gactgcacca tcttatattg
    13681 cattacttgg atggttaatc atcggtttaa tattctttgt cattcgatat cctaaattga
    13741 aaaatatgga taatgatgaa ttaagtcgct tgattttaaa tagaagtgaa aatgaagttg
    13801 atgatatgat tgaagaacct gaaaaagaaa aaactaagta ataaaagaat cgcacaatat
    13861 accttcttaa ttcggaggcg tatcgtgcga ttttttgtat tataaattga catttaagac
    13921 gaggcagttg aaccgtatat aaaattgcta agagttaggg ctgagccatt tctaacaaat
    13981 atttataatc gtttaaaaga tttcacgaac ccagaaacaa ttaatttgga aatttggtcg
    14041 gcgaataata aacctaatgc gatagctcct gcaataagtg taacctctag catggtattg
    14101 attgctgtac tgaaatttaa taatactaaa tttttcgttg catcgtaagc taatccacct
    14161 ggcactaacg ggatgatacc tgtcaccata aaaatgattg caggttcttt ttgtttacga
    14221 gccatatagt gacttaataa acctagggca agactgccga agaaactaga gtatatagtg
    14281 tgcacattaa agccgttgaa gaataaggtg taaaccatcc acccacacgt accaacgaaa
    14341 ccacatgata aatataattt tctaggtgca tcaaaaatga cgcagaagaa cattgaagct
    14401 aaaaagctaa agataaagtt taatatccaa aacatagtct gatactccta tactaaaatt
    14461 aatacgctac caacgccagc accgatgcca aacgcagtaa ccaatgcttc taatgatttc
    14521 gttgtgaaca tcaacatgtg cccaccaaat aaatcttgta ttgcgtttgt tattaataca
    14581 ccaggaacaa taggcatgac tgccgcaatg ataatagttg ccaagtcacc tgttggaata
    14641 agtgtatgtc caataacggc gataatccca ataactaatg aaccaatgaa ttctgggata
    14701 aactgtgcgt gtaacttacg atctaaaatc tcagtgacta ggtatcctag actacctgct
    14761 aatatcgcag ttaaaacatc aatcaatcta ccaccttgta aatataagaa actcattgca
    14821 atcattgctg cagcaaaacc tttaaaggga agactgctgt cacgcttggc aacatatatt
    14881 ttttcaagtt gtgtttttgc ctcggctaaa gaaatttcat tatttgtaat ttgacgcgaa
    14941 attttattag cttgagaaat ttttattaag tttgtatctc gagaggtaat tctaaatatt
    15001 ctaggaaacg attccgaatg taacgtaaac tggatgacag tgtttgtaac aaagctgtta
    15061 ctttcactgt aaccaagttt ttttgcaata cgtgtcatgg tatcttctac acgcgtacct
    15121 tctgcaccag attctaatag tatgcgagca gcaagcatga caacatcttt gataagtacc
    15181 tcttgtttgt attcttctga atttatgtcc atttcatcac cattgtttat aagaatttaa
    15241 tactcattat agtttataca ctataaaata accacatgag ctttttgata agttgttatt
    15301 gattttaaaa aactaatatt tgatactatt gtaataataa tcatcggaat ttttagtagt
    15361 taaaattctc ttgaagtata aaaaggaaaa attattgaaa aaaattcgac aaaaatatgt
    15421 ttcaaatatt tcaaataggt taaaataata ttagttaacc attacaaaaa ttgtatagag
    15481 tagcgactgt ataatttcta ttgaggttaa cgtttatatg tagtgatagt agttaaagtt
    15541 ctcccaagga agactactcg ggtacacttt gctatgagca aagtgtactt tgttattgat
    15601 aatacattag cacatatata taagtttaaa catacagatt tcaactattt atcaaatcac
    15661 gtcttatgta tacggaaata tactgaagat aaacgaaaaa ttccaagctt atcggtttaa
    15721 gcttggaatt tttttattaa tttataaacg taccaatgta ttaagaaatc gcaaagaatt
    15781 gatcgaattc gtttgtgtta ataatatgtc ctacaaagaa actaccgaat tcaccgtatc
    15841 gtgctgttgt ttcatcaaag cgcatttcgt atacaatttt tttgaattgt aatacgtcat
    15901 ctgagaacaa tgttacgccc cattcgaaat catcaaaccc tacagaacca gtaataaatt
    15961 gtttgatttt gccagcatat tttctaccaa tcataccatg gtcatacatt aatttttggc
    16021 gttcttccat agttaacatg taccagttat aagtttcatt acgacgtttg ttcattggat
    16081 agaaacaaat ataatcagaa tgtggtaatt ctgggtataa tcttgctttg atatgagggt
    16141 tctcataagg atcttcatca gatttaccag ctaaataatt gctcaattca atgactgata
    16201 catatgaata tgtagggatt aggaagtcag caatgcgcaa tttgttaaat tcattttcaa
    16261 tatgatttaa agacttcatt tcaggacgta agaaccataa taacaaatct gctttttgac
    16321 cagttatatt ataaatagct tgatcaccag attttgatga tcttacagtt gctgtatttt
    16381 ctaaaaatga ttgaaattca gtgacaagtg catcgcgttc gtcctttgga actagacgta
    16441 atgatgccca atcaactgca taaaataaat gtagactata ccaaccatct aatgtttcgg
    16501 ctgcttgact catgtttatc gctccttttc aaaaatcatc agttctttat aaattttatc
    16561 ataaagcaaa ggggtaacga taaaattatg attacaaatt ggtgacgtgg cattatgaaa
    16621 taaaatggcg tataattata ccgtgaatga ttaataagat ttatattaca ggaggacatt
    16681 atggctgatt tattaaatgt attaaaagac aaactttctg gtaaaaacgt taaaatcgta
    16741 ttacctgaag gagaggacga gcgtgttcta acagctgcaa cacaattaca agcaacagat
    16801 tatgttacac caatcgtgtt aggtgatgag actaaggttc aatctttagc gcaaaaactt
    16861 aatcttgata tttctaatat tgaattaatt aatcctgcga caagtgaatt gaaagctgaa
    16921 ttagttcaat catttgttga acgacgtaaa ggtaaagcga ctgaagaaca agcacaagaa
    16981 ttattaaaca atgtgaacta cttcggtaca atgcttgttt atgctggtaa agcagatggc
    17041 ttagttagtg gtgcagcaca ttcaacaggc gacactgtgc gtccagcatt acaaatcatc
    17101 aaaacgaaac caggtgtatc aagaacatca ggtatcttct ttatgattaa aggtgatgaa
    17161 caatacatct ttggtgattg tgcaatcaat ccagaacttg attcacaagg acttgcagaa
    17221 attgcagtag aaagtgcaaa atcagcatta agttttggca tggatccaaa agttgcaatg
    17281 ttaagctttt caacaaaagg gtctgctaaa tcagacgacg tgacaaaagt tcaagaagct
    17341 gtcaaattag cacaacaaaa agctgaagaa gaaaaattag aagcaatcat tgatggcgaa
    17401 ttccaatttg atgctgcgat tgtaccaggt gttgctgaga aaaaagcgcc aggtgctaaa
    17461 ttacaaggtg atgcaaatgt ctttgtattc ccaagtttag aagctggtaa tattggttac
    17521 aaaattgtac aacgtttagg tggatatgat gcagttggtc ctgtattaca aggtttaaat
    17581 tctccagtaa atgacttatc acgtggctgc tcaattgaag atgtatacaa tctttcaatt
    17641 atcacagcag cgcaagcctt acaataacga tggatttagc gagtaaatat tttaatggcg
    17701 tcaactggcg atatatcgat cattcttctg gattagagcc tatgcaatct ttcgcattcg
    17761 atgatacatt ttgtgaaagt gtgggcaaag atatatcaga taatgttgtg cgtacttgga
    17821 ttcatcaaca tactgttatt cttggtattc atgattcaag attgccgttt ttaaaagatg
    17881 gcattgatta tttaacgaat gagattggtt ataatgccat tgttagaaat tctggcggcc
    17941 taggtgtcgt tctagatcaa ggtgtattaa atatatcgct gatgttcaaa ggacaaacag
    18001 aaacaacgat tgatgaagcg tttactgtga tgtatctctt aattagcaaa atgttcgaag
    18061 atgagaatgt tgatattgat acgatggaaa ttgaacattc ttattgccca ggaaaatttg
    18121 acttaagtat cgatggtaag aaatttgcag gcatatcgca acgaagagtt agaggcggta
    18181 ttgctgtaca aatttatctt tgtgttgaag gctctggttc agaacgtgca ttgatgatgc
    18241 aaacatttta tgaacatgct ttaaaaggtg aagtgactaa attcaaatac cctgaaattg
    18301 aaccatcttg tatggcctca ctagagacat tacttaacaa aacgattact gttcaagatg
    18361 taatgttttt actattatat gcaatcaaag atcttggcgg tgtattaaat atgacgccaa
    18421 ttactcaaga agaatggcaa agatacgata cgtattttga taaaatgatt gaaagaaaca
    18481 agaaaatgat agaccaaatg caatagttaa atatttaaca gcccctttca agttatcgtt
    18541 tggggctgtt atttttgaaa gtatccataa atagcataga tatgttatta acctaatatt
    18601 taaattgaac atcattaaat taaaagtaat gatatgattg aaataataaa tttaataagg
    18661 aaatttcatg ttttacgtat taaatagatt gagaataggg tatatataat gcgtatgact
    18721 atgaaagaaa cgaccttcta ttgaaatgtt ctaatacttt agtagtaaat ctatatattc
    18781 caattattat ttgtagtatt atagttaggg tggaaaatta taaattgtag ggctatttta
    18841 taaaaagata aagaatctga aatatcactc gataagattt aaactttgaa gtaaacgaaa
    18901 gttagtttta tactaatcaa gttaagtcat atataaattt aaaataaagt ttatacataa
    18961 tatgaataat gtgagataaa gactgcaatg aaacttaacg cgaatgcgta tgcaacttag
    19021 aatatgatgt cttttactaa taaataaaaa caatgtatca agtataggat aaatataaaa
    19081 ggtgatattg acatgacaag aaaaggatat ggggaatcga caggtaagat tattttaata
    19141 ggagaacatg ctgttacatt tggagagcct gctattgcag taccgtttaa cgcaggtaaa
    19201 atcaaagttt taatagaagc cttagagagc gggaactatt cgtctattaa aagcgatgtt
    19261 tacgatggta tgttatatga tgcgcctgac catcttaagt ctttggtgaa ccgttttgta
    19321 gaattaaata atattacaga gccgctagca gtaacgatcc aaacgaattt accaccatca
    19381 cgtgggttag gatcgagtgc agctgttgcg gttgcttttg ttcgtgccag ttatgatttt
    19441 ttagggaaat cattaacgaa agaagaactc attgaaaagg ctaattgggc agagcaaatt
    19501 gcacatggta aaccaagtgg tattgatacg caaacgattg tatcaggcaa accagtttgg
    19561 ttccaaaaag gtcatgctga aacgttgaaa acgttaagtt tagacggcta tatggttgtt
    19621 attgatactg gtgtgaaagg ttcaacaaga caagcagtag aagatgttca taaactttgt
    19681 gaggaccctc agtacatgtc acatgtaaaa catatcggta agttagtttt acgtgcgagt
    19741 gatgtgattg aacatcataa ctttgaagcc ttagcggata tttttaatga atgtcatgcg
    19801 gatttaaagg cgttgacagt tagtcatgat aaaatagaac aattaatgaa aattggtaaa
    19861 gaaaatggtg cgattgctgg aaaacttact ggtgctggtc gtggtggaag tatgttattg
    19921 cttgccaaag atttaccaac agcgaaaaat attgtaaaag ctgtagaaaa agctggtgca
    19981 gcacatacat ggattgagaa tttaggaggt taatgcgttg attaaaagtg gcaaagcacg
    20041 tgcacatacg aatattgcac ttataaaata ttggggtaaa aaagatgaag cactaatcat
    20101 tccaatgaat aatagcatat ctgttacatt agaaaaattt tacactgaaa cgaaagtcac
    20161 ttttaacgac cagttaacac aggatcaatt ttggttgaat ggtgaaaagg ttagtggcaa
    20221 agaattagag aaaatttcaa aatatatgga tattgtcaga aatagagctg gcatcgattg
    20281 gtatgcagaa attgaaagcg acaattttgt accaacagca gcaggattgg cttcatcggc
    20341 aagcgcatat gcagctttag cagcagcttg taatcaagca ctagacttgc agctgtcaga
    20401 taaggattta tcgagattgg cgcgaattgg ttcgggttct gcatcgcgta gtatttatgg
    20461 tggatttgca gaatgggaaa aagggtataa tgatgagacg tcatatgccg ttccacttga
    20521 atcgaatcat tttgaagatg accttgccat gatatttatt gtgattaatc aacattctaa
    20581 aaaggtacct agtcgatatg gtatgtcgtt gacacgaaac acatcaaggt tttaccaata
    20641 ctggttagat catattgatg aagatttagc tgaagcaaaa gcagcgattc aagacaaaga
    20701 ttttaaacgc cttggtgaag taattgaaga aaatggtttg cgtatgcatg ccacgaatct
    20761 aggatcaaca ccgccgttca catatcttgt gcaagaaagt tatgatgtaa tggcgctcgt
    20821 tcacgaatgc cgagaagcgg gatatccgtg ttattttacg atggatgcgg gtcctaatgt
    20881 gaaaatactt gtagaaaaga aaaacaagca acagattata gataaattat taacacagtt
    20941 tgataataac caaattattg atagtgatat tattgccaca ggaattgaaa taattgagta
    21001 aggaagagat aaaatgattc aggtcaaagc acccggaaaa ctttatattg ctggagaata
    21061 tgctgtaaca gaaccaggat ataaatctgt acttattgcg ttagatcgtt ttgtaactgc
    21121 tactattgaa gaagcagacc aatataaagg taccattcat tcaaaagcat tacatcataa
    21181 cccagttaca tttagtagag atgaagatag tattgtcatt tcagatccac atgcagcaaa
    21241 gcaattaaat tatgtggtca cagctattga aatatttgaa caatacgcga aaagttgcga
    21301 tatagcgatg aaacattttc atctgactat tgatagtaat ttagatgatt caaatggaca
    21361 taaatatgga ttaggttcaa gtgcagcagt acttgtatca gttataaaag tattaaatga
    21421 attttatgat atgaagttat ctaatttata catttataaa ctagcagtga ttgcaaatat
    21481 gaagttacaa agtttaagtt catgcggaga tattgctgtg agtgtatata gtggatggct
    21541 agcgtatagt acttttgatc atgaatgggt taagcatcaa attgaagata ctacggttga
    21601 agaagtttta atcaaaaact ggcctggatt gcacatcgaa ccattacaag cacctgaaaa
    21661 tatggaagta cttatcggtt ggactggctc accggcgtca tcaccacact ttgttagcga
    21721 agtgaaacgt ttgaaatcag atccttcatt ttacggtgac ttcttagaag attcacatcg
    21781 ttgtgttgaa aaacttattc atgcttttaa aacaaataac attaaaggtg tacaaaagat
    21841 ggtgcgtcag aatcgtacaa ttattcaacg tatggataaa gaagctacag ttgatataga
    21901 aactgaaaag ctaaaatatt tgtgtgatat tgctgaaaag tatcacggcg catctaaaac
    21961 atcaggcgct ggtggtggag actgtggtat tacaattatc aataaagatg tagataaaga
    22021 aaaaatttat gatgaatgga caaaacatgg tattaaacca ttaaaattta atatttatca
    22081 tgggcaataa atgatttgat aggggagcaa tcatctaatt taattagaag ataaatgctc
    22141 cattttttat tgccagccgt gttacgtatc ttgaaaaaaa tattaaaaag agtaaaatag
    22201 atagagttaa cgaaaaaatt aatgaaatcg atgatataga tatgataaag aaaggtgggt
    22261 agcaatatga aaaatacatt ccttatttgt gatgaatgtc aggcagtcaa tataagaacg
    22321 ttacaaaaga agttggaaaa attagatccc gatgctgaaa tcgtgatagg ttgtcaatct
    22381 tattgtggac ctggacgccg aaaaacattc acttttgtta ataaccgccc actggctgcg
    22441 cttactgaag aagaattaat cgaaaaagtt tctcaacaat taaagaaacc acgtgatcct
    22501 gaagaagaag agcgtttaag aaaacgacat gaagaacgta aacgtcgtaa agaagaacaa
    22561 gatagaaagc ttaaagaaaa attagaaaag cgaaaagcac aacaataaag cctgatggca
    22621 gcatcattca atgcgtgcca ccaggttttt atgttttgtc tagaaattaa ataaatcatt
    22681 aaatgattcg gccatcgtag gatgcgtata aatattatct cgtaatacgg tatatggaat
    22741 gttttgatca atcgcaagtt taattatatt aattaattct tcagattgct taccatataa
    22801 tgtagcacct aaaatcatat tattttcatt attaatgact actttaaata aacctcttgg
    22861 atcattgtta attttgtgac gaggtatagc acttactaaa agttgatgtt cagtgtaatc
    22921 ataatgttga gcgacagctt ctttactagt taatccaaca cgtgataatg gtggatctat
    22981 aaatactgta taaggcacgc tgcctctatt gtcagtcgta cgtgactgat taccatataa
    23041 cgctgatttg ataattcgat aatcatctaa agatatatac gtaaattgaa gtccgccttt
    23101 aacatcacct gcagcataaa tatgcggcac agttgtttga agttgagcat tgactttaat
    23161 ttcgcctcta tcgcctaatt cgatatcagt attttctaaa gctaaatccg tattcggttt
    23221 gcgtccgata gccaaaagta ctgcatcagc ctcaaagttg ccaacattag tatggactgt
    23281 tgtatgatga tcgtcagatg acaattcagt cgtttcaaca tttgtatgca atgcaatacc
    23341 tttattttct aagtcagtaa tagcatgcgc aacgacatct tgatcttcgc gtggcataaa
    23401 tgattcacca cgttctaata cagtaacctt actacctaaa ttcgcaaaca ttgaagcaaa
    23461 ttctaaggcg atataaccgc cacctacaat aacgaggtgc ttaggttgat agctaatgtt
    23521 taataaacct gtcgaatcga agacgtgttt agcttgatca aggcctttaa tgttaggaat
    23581 gacagaggta gcaccggtat taataatgat atgaggtgca gtaatactat cgacgatatc
    23641 gccatgttga tctaataaat tcacttcagt attagattta aactgcgctt taaaatccag
    23701 tacatcaatg ttgttatcgt ctgctaataa gtggtaattt ttattgttta gcgcattgac
    23761 aacatcgttt ttacggttat aacttgcttc aaaagatttg ccttctaatc catcatgtac
    23821 aagtgtcttc gaaggtatac atcctatatt tatacaagtg ccgccataca ttttcgaaga
    23881 ttgttcgata actgcgacgt gttgacctgt tgatgcagca tatttggcta aagttttacc
    23941 agctttccca aatcctatta caattaaatc atatgttttc atgacataaa tcctcctttt
    24001 gaatgtcttc aatgacatct ttgattgttt tttctttata aaaattaata atgatattct
    24061 gttcgtcttg ttgataatga gacatggtag ttgcaatatt acgagcaatt tgacagtgac
    24121 tgccttcgtc gccagtaaat agtcgtgtgt gatgttcttt ctctaagaca aaatgtttat
    24181 ataatgttgc taaagagaca tcagcgctac gatcatttgc taaatatcca ccatctttac
    24241 ctcgtattgt gtcaatcatt tttaaatcga caagttgagt cgtcacgcgt cgtaattgaa
    24301 caggatttaa acaagttaat tctgctaatg aactactatt gaatttttct gaatgatgct
    24361 tagttaaaaa agctaataca tgcacggcaa tgttaaattc taaattcaat gtttctcacc
    24421 tcataattgt aactgtatta gttacaatta aaaatcattt tgctcaatgt gtcaatactt
    24481 atgtttgaaa agtgttgaat taggtgtggt tattgtgatg atttattgtt tgaaagttga
    24541 tgcattaacg gtgagattgc acaattgaaa ttttaattat gtatgaactg atagatatga
    24601 aatgaatatt gaaaatgaga gcaataaaat tttgaatgat gatgttgtgt gattgtataa
    24661 acttattcgt attttagata cgttttgata tttcgcatga tgaggaaata cttttaacaa
    24721 ctgatgacat attttaatag tgaatatatt ttgagatatt aatatattta atgttaagga
    24781 tgttgtcgca ataaaattaa aatattgaaa gataattttt attgattaat tgcgaatata
    24841 caaagacacc attatatcca tatgctaaaa taagaaaaaa ggcgacatgt tatctcaatt
    24901 tacctgcatc tagactcctt tacataagaa acaatcatcg ccgagtttta aaattttaca
    24961 actttgtaga tgtaaaaagc atttgatttt aaatagtttg aaaatcatat atttttgaaa
    25021 aaggagcatg ccgatgagta ttgacatgta tttagacaga tctcgaaacc aagcttcaag
    25081 tgtggggaat ttgagtcaaa caatgaattc aaattatgat gcgttggaaa aagcaattac
    25141 tcaatttatc aatgatgatg cgcttaaagg gaaagcgtat acgtcagcta agcaattttt
    25201 tagtacggtg ttaattccat tatcaacaag tatgaaaaca ttgagtgatt taacgaagca
    25261 agcttgcgat aattttgtgt cacgttatac gagtgaggtt gatagcatat ctttaaaaga
    25321 atcagagctt gaagaagata tcagatcatt aagtcaacaa attacgcgat atgaaaattt
    25381 gaataacaat ttgaaaaagc atgcttccga taatcagcaa gccatttcat cgaaccaaca
    25441 aataatacaa acattaggtc aacaaaaaca tgaattagaa gagaagctac gcaaattgcg
    25501 tgagtttaat caaaaatcac cagaaatatt taaagaagtt gaagaatttc aaaaaattgt
    25561 ccaacaagga cttacccaag cgcaaaattt ttggaacttt tcaacaaatc aatttaatat
    25621 tccttcaggt aaagaacttg attgggccaa agcaagtcat gaaaaatatt tgaaagttgc
    25681 tatggggaaa attgaacata aagcagagaa agaaacttta aataaagcag actttgctgt
    25741 tataaaggca tatgccaaag agcatccaga agacgatatc ccgaaaagta taatgaaata
    25801 tataaatgac aataaagaca gtattaaacg agatatagga ttagatatca catcaacact
    25861 tttagagcaa ggcggtataa atgcaagtaa attcggtgta tttatcaata cagcaggtgg
    25921 agtgaaaggc ccagcaggtc caaattcatt tgtggaagtc aaacgtacat cgggtaatgt
    25981 gtttatagaa aatggtagta aatttgcaaa aggcggaaaa tacctaggta aaggtgttgc
    26041 tggtgtagga tttggtatag gtatgtatga tgatatggca aacgatgata aaacagttgg
    26101 agaggcattg tcgcataatg ctttaactac aggaataggt actggtgctg gaattgctac
    26161 ttcagcaaca atagtcgcac ttgctagtaa cccagttggt tgggctgctt taggaggttt
    26221 ggcagtaggt attatctttt ctaatcttac agacttggca tatcaaaata attttcttgg
    26281 aataaaagac aaaacggatt gggtaggaca taaaatagac gatggtattg atgctgttaa
    26341 aaaatctaca gaaaaagcag tagatagttt tgagaatgct gttggtgaag ctacaaaagg
    26401 tatttctagt catattaacc ctatgaaatg gagctggtaa atattgcttt gtgaaaccga
    26461 aattatcaat aaaaatccaa aatatagagt tattaagtat gatgatgagt acttaatggt
    26521 cgatgtaata agcacttggc ttgtatactt ttttccattt attaattggt tcattccaaa
    26581 aaggtgcgcc aaaattagta gagaagagta tgaaaagctg aacactgtta agccagttaa
    26641 aaataaaact ttttggcctg ttgtaggagg aacaatctta ctgggtgtta cttccagaaa
    26701 gtacatacac ttacttaata ttcaattaga aaaaatattg gttataatta tatgcttcgt
    26761 tgtatttcta tgtattttaa tcttttttgt gcttctgaat cgaaaattaa aattgaaagt
    26821 tttcgataat aaaatagaag ataaaaaaat aatcttagta ccaacattaa aaaatgctgt
    26881 tcttatatta tatagctatt tgttgattgg aggcatgtca attttagctt taagcatgtt
    26941 actgacatta gaaaaccaaa atttaataac ttttatagct tggggtattg ggttaatgtt
    27001 gtttttcttg atgaatataa ctctcattgt taataaaaaa gccaaagtta taaaaaggtg
    27061 aataaataac taactatatt gtattggccg ttcttcaaat aaaaaataat ttgaataagg
    27121 tggaagtgat aaacagtgct atgtgaatct aaagttatca acaaaaatcc taagtataga
    27181 gttattaaat atggtgatga atatttaatg gttgatttag taagtacctg gttaacttta
    27241 tttcttccta tgattaattg gttaattcca aaaaaatatg tcaaaatcag taaaaaagaa
    27301 tttgacgatt taaacattgt caaacctgtt aaaaataaag ctttttggcc agttgcaggt
    27361 agtactattt tgttcggagt tacgtttaga aaatatattc cttcacttaa tattcaatta
    27421 gagaaaaaca tggtgattgt aatatgttgt gcaatatttc tgggtgtttt aatacttttt
    27481 ttatttctga atcgtaagct aagattggaa atttataata ataactctag taaagggaaa
    27541 ataattttat ttccttcatt aaaaaacttt tgcttcacaa tattttatta ttttttattt
    27601 ggtggtcttt caataatggc tctaagtatg ttattaactt taaatcctca aaatataata
    27661 ggctttattg gttggtaatg actgcaggtt tctttttgtt aaacatgtca tcgattattg
    27721 acaaaaaaat ttatgtatta tctaaaaata acatggtgga aaaatgacga ttttagctgg
    27781 atttattgtg ggttctgttt cgacaatact tgtatattgt accaatcaaa aaatgaattt
    27841 ggaataaaag ataaaacata ttagataggt catcaaacaa gatgacaata cagatgttgt
    27901 agaaaatatc gcaaatgata ttgtagatac tattaaaaac gccattggtg aaggtacaaa
    27961 aagtatttgg aatcattaaa tctaatgaaa tgagagtggt aaatgttgct gtgcgaatct
    28021 aaaatcatca ataaaaaccc aaaatataga attattaaat ataatgatga gtacttaatg
    28081 atcgatataa taagtacttg gattagtttg ttttttccat ttatcaattg gttcatccca
    28141 aaaagatacg tcaaaattag tagagaagag tttgaaaatt taagtattgt taaacctgct
    28201 aaaaagaatg ttttttggcc agttgcaggt atctctactt tattcgcagt tacattaaga
    28261 aagtatacgc atttacttga cactcaactt gataaaaaat tagttattgc catatgttgt
    28321 atcacattta tagggatttt aatattttat gtacgcctaa ttaaaaaatc atctttaaat
    28381 atttataata ctaaaaataa aaggtcgaaa atcatcttaa taccaacact aaaaaatttt
    28441 tgtttcacat tgttcggata tgttttttta attttttgga cagtggtttt ctcatatgct
    28501 ctattatcaa tgagttatca aaacataata gtatattttg cttggattac agcaata
//
LOCUS       DAPIDM010000020        27044 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000020 DAPIDM010000000
VERSION     DAPIDM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.020"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..344)
                     /locus_tag="RJE42_000782"
     CDS             complement(<1..344)
                     /locus_tag="RJE42_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV5876227.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGEFAGKVTQKNVDKKYIE
                     TLTNSAELGIISQKDYFDKEISNIDNIKKYYVVEENDFVYNPRMSNYAPFGPVNRNKL
                     GKKGVMSPLYTV"
     gene            complement(337..1893)
                     /locus_tag="RJE42_000783"
     CDS             complement(337..1893)
                     /locus_tag="RJE42_000783"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDV5876228.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEVEQEINAY
                     LKELGVLKDE"
     gene            1953..2072
                     /locus_tag="RJE42_000784"
                     /pseudo
     CDS             1953..2072
                     /locus_tag="RJE42_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2155..2838)
                     /locus_tag="RJE42_000785"
     CDS             complement(2155..2838)
                     /locus_tag="RJE42_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDV5876229.1"
                     /translation="MKLTALAKVTLAIGILTTGTLTTEAHSGHAKQIHKSVNKHDKEA
                     LHRYYSGNFKEMKNINALKHGKNNLRFKYRGMKTQVLLPGDEYRKYQQRRHTGLDVFF
                     VQERRDKHDISYTVGGVTKTNKTSGFVSTPRLNVTKEKGEDAFVRGYPYDIKKEEISL
                     KELDFKLRKHLIEKYGLYKTISKHGSVKINMKDGSFYNLNLRCKLAFKYMGEVIDSKQ
                     IKDIEVNLK"
     gene            complement(3205..3903)
                     /locus_tag="RJE42_000786"
     CDS             complement(3205..3903)
                     /locus_tag="RJE42_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDV5876230.1"
                     /translation="MKLTAIAKATLALGILTTGVMTAESQTVNAKVKLDETQRKYYIN
                     MLKDYYSQESYESTNISVKSEDYYGSNVLNFNQRNKNFKVFLIGDDRNKYKELTHGRD
                     VFAVPELIDTKGGIYTVGGITKKNVRSVFGYVSHPGLQVKKVDPKDGFSIKELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGTADKGRIVINMKDEKKHEIDLSEKLSFDRMFDM
                     LDSKQIKNIEVNLN"
     gene            complement(4244..4939)
                     /locus_tag="RJE42_000787"
     CDS             complement(4244..4939)
                     /locus_tag="RJE42_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDV5876231.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVKAAEKQERVQHLYDIK
                     DLHRYYSAPSFEYSNISGKVENYNGSNVVRFNQKDQNHQLFLLGKDKEQYKEGLQGQN
                     VFVVQELIDPNGRLSTVGGVTKSNNKTSETKTQLVVNKVDGGNLDASIDSFLIQKEEI
                     SLKELDFKIRKQLVENYGLYKGTSKYGKIIINLKDEKREVIDLSDKLEFERMGDVLNS
                     KDIRGIAVTINQI"
     gene            complement(5386..6090)
                     /locus_tag="RJE42_000788"
     CDS             complement(5386..6090)
                     /locus_tag="RJE42_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDV5876232.1"
                     /translation="MKMTAIAKASLALSILATGVITSTAQTVNASEHESKYENVTKDI
                     FDLRDYYSRASKELKNVTGYRYSKGGKHYLIFDKHQKFTRIQIFGKDIEKLKARKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNKGAYYDYLSAPRFVIKKEVGAGVSVHVKRYYI
                     YKEEISLKELDFKLRQYLIQDFDLYKKFPKASKIKVTMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(6451..7404)
                     /locus_tag="RJE42_000789"
     CDS             complement(6451..7404)
                     /locus_tag="RJE42_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDV5876233.1"
                     /translation="MRMTQIAKASLALGLLTTGASIVTTQSAKAEVTPSLKSEQLSMI
                     SVSPSAVTTTTQGVNAEVASALKTEQAIKSNIQKVTTSHSTNAQPQSTQSTPKPTTPP
                     SANVETPQPQSPTTKQAQKEINPKYKDLRTYYTKPSLEFEKQFGFMLKPWTTVRFMNV
                     IPDWFIYKIALVGKDDKKYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDH
                     KAELSITKKDEEGKISRDVSEYKITKEEISLKELDFKLRKQLIEQHNLYGNIGSGTIV
                     IKTKNGGKYTFELHKKLQEHRMADVIDGTSIDRIEVNLTSS"
     gene            complement(7751..8782)
                     /locus_tag="RJE42_000790"
     CDS             complement(7751..8782)
                     /locus_tag="RJE42_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDV5876234.1"
                     /translation="MKIRIIAKASLALGLVTTGASIVTTQSANAEVASALKAGQLAKI
                     SVSPSAITTTTQAVNAEQNRTANTEQASKSNTENVSTSPSTKTEQTPKSNIQKATQQT
                     PSVTTTPSPNVQPQSPQPTPKPTTPPSSNVETQQPQSPTTKQAQKEINPKFKGLRSYY
                     TKPSLEFKNELGIIIKKWTTIRFMNIVPDYFIYKIALVGKDDKKYGEGVHRHVDVFIV
                     LEQNKYGVDKYSVGGITKANRKKVDYNARIKITKEDKKGTISHDVSEYKITKEEISLK
                     ELDFKLRKQLIEQHNLYGNIGSGTIVIKMKNGGKYTFELHKKLQQHRMADVIDGTNID
                     RIEVNLKSS"
     gene            complement(9069..9770)
                     /locus_tag="RJE42_000791"
     CDS             complement(9069..9770)
                     /locus_tag="RJE42_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDV5876235.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTVTEQPVHAENKHVQVSQNSKNL
                     KAYYTQPSVEYKNVTGYISSIQPKPGTKFLNMIEGNTVNNLALVGKDKEHYHTGVHRN
                     LDIFYVNEDKRFEGAKYSIGGITKASDKVVDQVAEARVIKEDHTGEYAYDFFPFKIDK
                     EAMTLKEVDFKIRKHLIDHYGLYGEMSSGTVTVQMKYYGKYTIELDKKLQEDRMADIV
                     KVIDIDRIEVKVKKA"
     gene            complement(10054..10734)
                     /locus_tag="RJE42_000792"
     CDS             complement(10054..10734)
                     /locus_tag="RJE42_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDV5876236.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNIQSVQAKTEVKQQSESELKH
                     YYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQYSQISLVGSDKDKFKDGDNSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKGNEDVLKDLYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGTKI
                     DRILVEIK"
     gene            11206..12051
                     /locus_tag="RJE42_000793"
     CDS             11206..12051
                     /locus_tag="RJE42_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5876237.1"
                     /translation="MNIILTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVSVRQLDYFNQESMVKAFKGIDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLATSGIDYTYVRMAMYMDPLKPYLPELMKMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNEQPQTLQSF
                     LQENI"
     gene            12070..12429
                     /locus_tag="RJE42_000794"
     CDS             12070..12429
                     /locus_tag="RJE42_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDV5876238.1"
                     /translation="MNIISIILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINVLLKNQGVYNGLIGVLLVYGLFFSSNPKELCAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            12956..13108
                     /locus_tag="RJE42_000795"
     CDS             12956..13108
                     /locus_tag="RJE42_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876239.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFHNRIKNNPQKTNPFLKL
                     HENKKT"
     gene            13369..13641
                     /locus_tag="RJE42_000796"
     CDS             13369..13641
                     /locus_tag="RJE42_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV5876240.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIFSNSI
                     KQHQNTGSFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILELI"
     gene            14040..14744
                     /locus_tag="RJE42_000797"
     CDS             14040..14744
                     /locus_tag="RJE42_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV5876241.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     ITIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYRDIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            15137..15673
                     /locus_tag="RJE42_000798"
     CDS             15137..15673
                     /locus_tag="RJE42_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876242.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMVLVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(15786..17327)
                     /gene="guaA"
                     /locus_tag="RJE42_000799"
     CDS             complement(15786..17327)
                     /gene="guaA"
                     /locus_tag="RJE42_000799"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV5876243.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCECKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(17352..18818)
                     /gene="guaB"
                     /locus_tag="RJE42_000800"
     CDS             complement(17352..18818)
                     /gene="guaB"
                     /locus_tag="RJE42_000800"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV5876244.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(18856..20124)
                     /locus_tag="RJE42_000801"
     CDS             complement(18856..20124)
                     /locus_tag="RJE42_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDV5876245.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(20124..20702)
                     /gene="xpt"
                     /locus_tag="RJE42_000802"
     CDS             complement(20124..20702)
                     /gene="xpt"
                     /locus_tag="RJE42_000802"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV5876246.1"
                     /translation="MELLGQKVKEDGIVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(20933..21035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            21215..21622
                     /locus_tag="RJE42_000803"
     CDS             21215..21622
                     /locus_tag="RJE42_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV5876247.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDKMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVGV
                     RDHSSHQEVLENNSAYEEVDITHFAEASKGPKA"
     gene            21765..22427
                     /locus_tag="RJE42_000804"
     CDS             21765..22427
                     /locus_tag="RJE42_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876248.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            22545..23501
                     /locus_tag="RJE42_000805"
     CDS             22545..23501
                     /locus_tag="RJE42_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876249.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTTKIDSNSESLKAIQTLQEAIQ
                     EAQASQAKVLAESTNKVENNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIETASVEQSKNDKAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QVTSNDSSEAKAENDSTASQSEMAEPKPQEEKKGFFARLFNL"
     gene            complement(24312..24410)
                     /locus_tag="RJE42_000806"
     CDS             complement(24312..24410)
                     /locus_tag="RJE42_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876250.1"
                     /translation="MQLNVNIMKFDLWHKGICKQIVLNLVEKGEKG"
     gene            24495..25883
                     /locus_tag="RJE42_000807"
     CDS             24495..25883
                     /locus_tag="RJE42_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDV5876251.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLAYGTHSNIITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            complement(25963..26718)
                     /gene="nfsA"
                     /locus_tag="RJE42_000808"
     CDS             complement(25963..26718)
                     /gene="nfsA"
                     /locus_tag="RJE42_000808"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDV5876252.1"
                     /translation="MSDHIYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVARVREILDLPDYVFPLFG
                     MAVGEPAEDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
BASE COUNT         8511 a         4934 c         4089 g         9510 t
ORIGIN      
        1 aacacagtat aaagaggtga catgacccct tttttcccta acttatttct atttactggt
       61 ccaaatggag cataattaga cattctaggg ttataaacaa aatcattctc ttcaactaca
      121 tagtactttt taatattatc tatattcgaa atttctttgt caaaataatc cttttgagat
      181 atgataccta actcagctga attagttaat gtctcaatat attttttatc aacatttttt
      241 tgggtaactt taccagcaaa ctcacctagc ttcttctctt cccattcgcc ttcaaaccct
      301 gggaatctca actctggcac atttttcttt tgtgtattac tcatctttca acaccccaag
      361 ttctttcagg tatgcattga tttcttgttc aacttctgcg atttctttgt cgatattttt
      421 caaatcttgt tggacttgat ctaaatcaat tggcgcttct tcttcgaatg tatcgacata
      481 tctcggaatg tttaagttgt aatcgttatc ggcaatctct tgtaatgtcg cgctgtagct
      541 atatttatcg attgtttctt tacgcttata tgtgtctata atgcgttcga cttgggcgtc
      601 gcttaaatgg ttttgatttt ttcctttttc aaaatcattg gatgcatcga taaataatac
      661 gttgtcgtct tgttggcgac attttttaaa tactaaaata catgttggaa tacttgtccc
      721 atagaaaata ttcgctggta agccaatcac ggcttctagg tagttctttt cttcaattaa
      781 gtaacgacga atgacgcctt ctgcagcccc acggaataag acaccatgcg gtagtacaac
      841 tgccatggta ccttcatcat ctaggtaatg taccatgtgt tgaataaagg caaagtctgc
      901 tttagacttt ggcgcaagct tgccgtatcc gctgaatcgt tcgtcatttt caaattttga
      961 atctgctgtc catttcgcac tgtatggtgg gttcgcaata accgcatcaa atgtatggcc
     1021 taaaaaggct ggattttcca acgtgtcatc attacgtata tcgaaatttt cataacgtac
     1081 atcatgtaat aacatgttca tgcgcgctaa gttgtatgtg gtattgttac gttcttgacc
     1141 gaaataacga tacacttgcg tttctttacc tacacgtaac agtaatgaac ctgaaccaca
     1201 tgttgggtca tatacatgac gtaatttatc tttaccgtct gtgacaatct tcgccagtat
     1261 cttagatact tgttgtggtg tatagaactc gccagctttt ttacccgctg tcgccgcaaa
     1321 gcgcccgata aggaattcgt atgcatcacc taacatatca atttccatat cactgtgaac
     1381 aaatggtaag tcgtcaagat taaccatgac tttagagatt aacgcagtac gttctttgac
     1441 attgttacct agtcgcgttg aactcaaatc catatcgctg aatagtccga taaagtcatt
     1501 ttcactttct tcacctaatg ttgatgtttc aactttacga attgccgtcg ctagatgttc
     1561 gatatcgaaa tcttgcgttt caatttcacg aatcatcgcg ctgaataaat cttgcggttc
     1621 aataaagtaa ccaacttgat caattaattc tgcttttaag tcttcacggt attcttcatc
     1681 tgcccatgct tcttgatacg tgatgtcttc acctgacaag gcatctgcgt attcttgttc
     1741 cgctttttca gataagaagc gatagaaaat caagcctaaa atgtaattac ggaattcact
     1801 cgcatccata ttccctctta aatcattcgc aatcgaccat aattttttat gtaattcagc
     1861 ttgttgctga cgttgttttt cagtaataga catgtgattc ctccgccttt gaataagtaa
     1921 tttatctctt tgtgtaatag atttattata acatttggtt atgttgcgat gttgagaatt
     1981 ttgatgttgg tggttgaaat tttatcttta tggagattgg gataggattc ataacacgca
     2041 aacaaaaacg gcttaacaac gatgtcgatt gatccatcct tatttgtaag gtgtgcgtac
     2101 caatttcctt ttcatcgtta agccgcttca attcttttta ttatttgtaa ttatttactt
     2161 caaattcact tcaatatctt taatttgttt actatctatg acttctccca tatatttgaa
     2221 cgctaattta caccttagat taaggttata aaagctgcca tctttcatgt tgattttaac
     2281 actaccatgt tttgagatcg ttttataaag accatatttt tcaattaaat gctttctcaa
     2341 cttaaaatct aattctttca atgatatttc ttctttttta atatcataag gataacctct
     2401 cacaaaagca tcttcaccct tttcttttgt aacatttaat cttggtgtgc tgacaaagcc
     2461 ggatgtttta ttggtttttg ttacaccacc aacagtatat gatatgtcgt gcttgtctct
     2521 tctttcttga acaaaaaaca catctaagcc cgtatgtctt cgctgttgat atttacggta
     2581 ctcatctcca ggcaataata cttgagtctt catacctcta tatttaaaac gtaagttgtt
     2641 tttaccatgt ttcaaagcat taatgttttt catttcctta aagtttccac tgtagtatcg
     2701 gtgtaatgct tctttgtcat gtttgtttac tgacttatga atttgttttg catgacctga
     2761 atgggcctct gttgttaaag ttcctgtcgt taaaattcct attgctaatg ttacttttgc
     2821 taatgctgtc aatttcatat ttatttactc caatcttaat gtattggatt gttattatta
     2881 cgtaatttga atcatatacg cttattataa cgtccatcgt ttaacgcgat aattaacctc
     2941 gatttaaata tgcgtgaatt attattaatg aaaagcaaat atattttaat gttcatattc
     3001 aatgactttc acgctttaca tgtcgttgtt tggctgagtc actctttact acaactaatg
     3061 ttgatactct gttctgtact ttgaaatgca aaaaacaact taacaacaat aatgtattta
     3121 acacagttct tattcaaagg tacgggtgtc aatgcacatt ttatcgttaa gtcgcttttt
     3181 aattttttat actatcatct ttaattaatt caaattcact tcaatatttt taatctgctt
     3241 actatccagc atatcaaaca tacgatcaaa acttaatttt tcacttaaat caatttcatg
     3301 tttcttttcg tctttcatat tgataacaat tctaccttta tctgctgttc ctttatacaa
     3361 tctatatttt tcgactaaca tttttctgat tttaaaatca agttctttca atgatacttc
     3421 ttccttttga ataaagaaca actcttttat cgaaaagcca tctttaggat caactttttt
     3481 aacttgcagt cctggatgac ttacatagcc aaacacggat ctcacatttt tctttgttat
     3541 accgccaacg gtatatatgc cgcctttagt atctattaat tcaggtactg caaagacatc
     3601 acggccatgc gtcagttctt tatatttatt tctatcgtcg ccaattagaa atactttgaa
     3661 atttttattt cgttggttaa agtttaaaac gttagaccca taataatctt cacttttaac
     3721 actaatgttt gttgattcat agctttcttg agaataataa tcttttagca tatttatata
     3781 atatttgcgt tgtgtttcat ccaactttac tttcgcgttt acagtttgac tttctgctgt
     3841 catcacacct gttgttaata ttcctaatgc taatgttgct ttagctatcg ctgttaattt
     3901 catattttat tgctccaatc ttaatgtatt ggattgttat gattacgtaa tttgaaacat
     3961 ctgcctttat tataaagtgc atcgaaacaa actgacatta acgtgcgttt aaataacttt
     4021 gaatcattgt taatgaacac ctaagtaata ttcaaagcac gtagctttca cgtctgccat
     4081 acctttttaa gtatactcat gacctgtgtt attaaatgta aaaaacagcc taacgacggc
     4141 gttgctttaa atacgctcct ttggtaggat atacatattt attcaacgtt tcatcgttaa
     4201 gctgcctcaa aatttattac tttaaagtca ttgattgctt actttaaatt tggttaatag
     4261 tgactgctat acctctaatg tctttactat tcaacacatc gcccatgcgc tcgaattcca
     4321 atttatcact taaatcaatt acttctcttt tctcgtcttt caaattgata atgattttac
     4381 cgtatttaga tgtaccttta tataatccgt aattttcaac taattgtttt ctaattttga
     4441 aatcaagttc ttttaatgag atttcttctt tttgaattaa aaatgagtca atagatgcat
     4501 ctaaatttcc gccatcaact ttattaacaa ctaattgtgt tttagtttca gaagttttgt
     4561 tattgctctt cgttacacca ccaacagtag atagtctgcc gtttggatca attaattctt
     4621 gtactacaaa gacattttgg ccttgtagac cttctttata ctgttcttta tcttttccta
     4681 gtaagaataa ttggtgattt tgatcttttt ggttaaagcg tacaacgtta gaaccattgt
     4741 agttttcaac cttaccgcta atattactat attcaaaact tggtgctgag tagtatcgat
     4801 gtaagtcttt aatatcatat aaatgttgta ctctctcttg cttttctgct gcttttactg
     4861 cttgaccttc tgatgtaatg acaccagtag ttaataaacc taatgctaat gttgctttag
     4921 ctaacgtttt taatttcata gtattattct cccaatctat ttttaaattt tgacttaata
     4981 tatgtttatg tgattaattc aattacgtaa tacgaacaat ctactatcat tatataagcc
     5041 ggatactgcg ctatgaatta accgcttttt aactattcct gaaccaacgt taagctaatt
     5101 aatggaatcc taacagtatt aatcattttt tataactcta tgctattttt ttcaccattc
     5161 acactttcct tcaatttaat tatgttttcc acattgtttc atgtcactaa tgcgacaccg
     5221 tgcgactata ggtatcaact attttcacag gttttattgt cgttattatc tttcatcgcc
     5281 ccacttcatt tccgatcgta acgacacaaa aaagaagcta agcaacctat gctgcctagc
     5341 tcatctatac tatccatatt ttaccattat ccatgtttcg ttgaattatc taatattggc
     5401 ttctattttc tcaatatttc tgccatctat gacgtcactc attcggtttg tttgtaactt
     5461 tttattgagt tcaaatgtat aatagccgcc atctttcatt gtcactttta tcttgctagc
     5521 tttaggaaac tttttataca aatcaaaatc ttgaattaaa tactgtcgta atttaaaatc
     5581 aagttctttc agcgaaatct cttccttata aatgtagtat cttttaacat gcacagaaac
     5641 acctgcacct acttcttttt taataacgaa tctaggtgcg cttaagtaat catagtaagc
     5701 gcctttattt ttcttagtaa cgccaccata tgaatacact gtgccgttgc gattttccgc
     5761 ttctttaact acaaatatat ccaaacctgg atttttacgt gctttaagct tttctatatc
     5821 tttaccaaaa atttgtattc tagtgaactt ttgatgttta tcaaagataa ggtagtgctt
     5881 gccacctttg ctataacgat agccagtaac atttttaagt tccttacttg cgcgactata
     5941 gtaatctctc aagtcgaaga tatcttttgt cacattttca tattttgatt catgttcgct
     6001 cgcatttaca gtttgagccg ttgatgttat aaccccagtc gctaaaatac ttagagctaa
     6061 actggctttc gcaattgctg tcatcttcat agttgtatgc tccaatcgta attattagat
     6121 ttgttcgatt acgtttattg aatcatacag ctttattata gttggcgtat gtctctattc
     6181 atattaaacc ttgtttaact atatttgaat catcgttaat taaattaagg aatccataat
     6241 gttcgttaaa taaaaatgac ttttgttgta cttcaacgct tgatagattg gtatttttag
     6301 acacatttag tacataagta tgttatgtgc ggtcaacatc gaatattcgt cttcttatac
     6361 cactgcccaa cacgaaagaa gccgagtaac atgtaaatcg ttgtcactca acttctcatt
     6421 tcatcgttca acggttctat gcagtatcca ttacgatgat gttagattca cttcaattct
     6481 gtcgatacta gtgccatcta tgacatctgc catgcgatgt tcttgtaatt ttttatgcaa
     6541 ttcaaacgtg tacttcccac cgtttttcgt tttaataaca attgttcctg aaccaatatt
     6601 accgtacaga ttatgttgtt caatgagttg ctttctcaat ttaaaatcaa gctctttcaa
     6661 ggaaatctct tctttagtaa tcttgtattc tgaaacatcg cgtgagattt tgccttcctc
     6721 atctttttta gtaatgctta attctgcttt atgatcaact tttttactat tcgtcttcgt
     6781 gatacccccg acagaatatt tttttaattg atatttattg tcctctaaaa cgataaatac
     6841 atcaatatta tcgtaaggtc catctttata tttcttatcg tcttttccaa ctaaagctat
     6901 tttataaatg aaccaatctg gtataacatt cataaaccta actgtcgtcc atggtttcag
     6961 cataaagcca aattgctttt cgaattctaa actcggtttc gtataatatg ttcttaaatc
     7021 cttatattta gggtttattt ccttttgtgc ttgttttgtg gtaggtgatt gtggttgagg
     7081 cgtttcgaca ttagctgaag gtggtgttgt tggttttggt gttgattgtg tcgattgcgg
     7141 ttgtgcattt gttgaatgcg atgttgtcac tttttgtatg ttagatttta ttgcctgttc
     7201 tgttttaagt gctgatgcta cttctgcatt cactccttga gttgtcgttg tgactgcact
     7261 tggtgatacg cttatcattg ataactgttc tgatttaagt gatggcgtta cttctgcttt
     7321 ggccgattgc gttgttacta tacttgcacc tgttgttaaa agccctaatg ctaaactcgc
     7381 tttcgcaatt tgtgtcattc tcatagttgt atgctccaat ctattatatt cgattgttct
     7441 ttacgtaatt agaatcatac aacttcatta tagaagacgt attggtctat tcacattaaa
     7501 ccatgtttaa ctatatttga atcatcgtta agtaatccat aattttcgtt aaatacagat
     7561 ggtttcgaag cgattcaacg catgcaaatt gggcattgca tcattttcaa gacattgaga
     7621 tgtcactttt acaaacaagt aaatattttc ccttaacatt attacgcaaa acaaaagaag
     7681 ctaagcaatg atgctggcca ttgtcgctta acttctcatt tcatcgttca acggttctat
     7741 gcagtatcca tcacgatgat tttagattca cttcaattct atcaatattt gtaccatcaa
     7801 taacatctgc catacgatgt tgttgcaatt ttttgtgtaa ttcaaacgtg tacttcccac
     7861 catttttcat tttaataacg attgttcctg aaccaatatt accgtacaaa ttatgttgtt
     7921 caatgagttg ttttctcaat ttaaaatcaa gttctttcaa ggaaatctct tctttagtaa
     7981 tcttgtattc tgaaacatca tgtgaaattg tacctttttt atcttcttta gtaattttta
     8041 ttcttgcgtt gtaatcaact ttctttctat ttgcttttgt gataccaccg accgagtatt
     8101 tgtctacgcc atatttattt tgttctaaaa cgataaatac atcgacatgt ctatgcacgc
     8161 cttcaccata tttcttatca tcttttccaa ctaaagctat tttatatatg aaataatctg
     8221 gaacaatatt cataaatctt attgttgtcc attttttgat aattataccc aactcatttt
     8281 taaattctaa acttggtttc gtataatatg atcttaaacc tttaaattta gggtttattt
     8341 ccttttgtgc ttgtttggtg gtaggcgatt gcggttgttg tgtttcgaca ttagatgatg
     8401 gcggagttgt tggttttggt gttggttgtg gcgattgcgg ttgtacattt ggtgaaggtg
     8461 ttgtagttac gcttggtgtt tgttgtgttg ctttttgtat gttagatttt ggtgtctgtt
     8521 ctgttttagt tgaaggcgat gttgatacat tttctgtgtt agactttgat gcctgttcag
     8581 tattagccgt acgattttgt tctgcgttca ctgcttgcgt tgtcgttgtg attgcgcttg
     8641 gtgatacgct tatctttgct aactgtcctg ctttaagtgc tgatgctact tctgcattgg
     8701 ccgattgcgt tgttactata cttgcgcctg ttgttacaag ccctaacgct aaactcgctt
     8761 tcgcaattat tcttattttc atagttgtat gctccaatcg taattattcg attgttcttt
     8821 acgtaattag aatcatacaa cttcattata gaggacatat tggtctattc acattaaccc
     8881 tcgtttaaca attgttgaag tattattaag tcatttaagc aaaaaataat gagtactttc
     8941 gaggtttatg tggtgttact acgtatcata gaaatgattt taagacaaaa aaagaagtta
     9001 agtaatgttt tatcatcact taacctcact attgcaattt caattcatta cgacgaccaa
     9061 tctaagtatt atgctttttt aactttgact tcaattctat caatatctat gactttgacg
     9121 atatcggcca ttcggtcttc ttgtaacttt ttatccaatt caattgtata cttgccataa
     9181 tatttcattt ggacagtaac tgttcctgaa ctcatttcac cataaagacc ataatgatca
     9241 ataagatgtt ttcttatttt aaaatcaacc tctttcaatg tcattgcttc tttatctatt
     9301 ttaaatggga aaaagtcata tgcatattca ccagtgtgat cttctttaat gactcttgct
     9361 tctgctactt ggtcgacaac tttatcgctt gcttttgtga taccaccaat cgagtacttt
     9421 gcaccttcaa atctcttatc ctcattaaca taaaatatat caaggttgcg atgtacaccc
     9481 gtatgatagt gttccttatc tttgccaact aaagctagat tattaactgt attaccttct
     9541 atcatattca aaaatttagt gcctggttta ggttgaatac tactgatata acctgtcaca
     9601 tttttatatt caacactagg ttgagtatag tacgctttta aatttttact attttgactt
     9661 acttgaacat gtttattttc ggcatgcacc ggttgttccg ttacagtatt tacacctgta
     9721 gctaaaatac ccaagactaa acttactttt gcaattgatt tcattttcat aattgtatgc
     9781 tccaatattt tagattagat tgttttatta cgtaatttga accatacaat catattatag
     9841 gagctgtatg ctgatattca cattaaccct tttttaacta ttcataaaat atgattaagc
     9901 tagttaagta aaagataatg cgtggtgcga ctgtttgcgt gagttttcta actttcctct
     9961 caaaaaaaga agctaagcaa caatacgtcg cttaacttcc atatatttcc ataacaagca
    10021 cgattcaaca tagcgctttg tcgttagaaa gtattatttt atttctacta gaattctatc
    10081 aatcttagtg ccgtcgattg agtcgcccat acgctctttt tcaagttttt gacttaaatc
    10141 gatagtatgt gatttgccat ctttcatagt aattgtaatt tgaccttgtt taagaccgtt
    10201 tgaatacaag ccgtgttgtt tgattgcacg ttctcgtaat ctataatcaa gttcttttaa
    10261 tgagatgtct tcttttgaaa tgtagtacaa atctttaagt acatcttcat tacctttctt
    10321 gatttctaaa attggcgtat tgatataatc aatatactgc acactatttg attttgtaac
    10381 gccaccaatt gagtaatttg ttgcttgtct actgtcacct tctctaagga taaacacatc
    10441 tatattcgag ttgtctccat ctttaaattt gtctttatca gatccaacta aagaaatttg
    10501 agaatattga ttgtctacta taacatctat ataatcttta cctttttcag tatatttata
    10561 tccagtaaca tttttacgct ctaaaaccgg tttattataa tagtgtttca actctgattc
    10621 actttgttgt tttacttctg ttttcgcttg tactgattgt atattcgacg ttattacacc
    10681 tgttgctaac attcccaatg ctaaacttgc ttttgctatc gctttaaatt tcataatttt
    10741 tagccccaat ctatttaaat ttgatttttc aattacgtaa tatgaacaat ctacctacat
    10801 tatattgagg cgctattccc tttcaaatta aacctcattt aactaaactt gaatcattgt
    10861 taaggtaatt aataaaatgc taactgttat taatcaattc ttatccgtcg agactcattt
    10921 ttatacaatt cacactttat accattttaa ttttaaaaac tgtaaaactt tcagacacaa
    10981 aaatgacatc atatcgtcgc agtaccatgt caaaattttc aaagatcatg gtgacacgca
    11041 tcaactaaaa cgcgtcatta cggtattttt aacattaata acatatgcgt atttactata
    11101 cacggcgaca ataaatacag attgcatttt tttataaaat caaatgtttt gaaaatcatc
    11161 gaataagtat tatgttatgt atataaagat taggagatga agtgaatgaa tattatatta
    11221 acaggtgcta caggtcattt aggcacacat attacaaatc aagctattgc aaatcacata
    11281 gatcattttc acattggtgt tagaaatgtt gagaaagttc cagaagattg gcgcggaaaa
    11341 gtttctgtgc gacaattaga ttattttaat caagaaagca tggtaaaagc atttaaaggc
    11401 atcgacaccg ttgtgtttat tccaagtatt atccatccat catttaaacg tattcctgaa
    11461 gtggaaaatt tagtatatgc cgcaaagcaa agtggcgttg ctcatatcat tttcataggt
    11521 tattacgcag atcagcataa taatccattc catatgagcc cttatttcgg atatgcatca
    11581 cgtttattgg caacaagtgg cattgattat acgtatgtaa gaatggcaat gtacatggat
    11641 ccacttaaac catatttgcc agaattgatg aagatgcata aactgattta tccagctggt
    11701 gatggtcgta ttaattatat tactagaaat gatattgcta gaggtgtcat tgctattatt
    11761 aaaaatccag atacttgggg caaacgttat ttattatcag gctacagtta tgatatgaag
    11821 gaacttgctg caattttatc tgaagcatca ggcacagaaa ttaaatatga acccgtttca
    11881 ttagagacat ttgcagaaat gtacgatgaa cctaaaggct ttggtgcatt attggcatca
    11941 atgtacgacg caggagcaag aggattatta gaccaagaat ccaatgattt ccaacaatta
    12001 gtcaatgagc aaccacagac actgcaatca tttttacaag aaaatattta atactaaagg
    12061 agcgttacag tgaatatcat ctcgataatt ttaatcatat ttgtggcatt agaatttttc
    12121 tatattatgt accttgaaac gattgctaca acttccaaaa agaccagcga gacatttaat
    12181 ataagcgtcg ataaattgaa agacaaaaat attaacgtac ttttgaagaa tcaaggcgta
    12241 tataacggtt taatcggagt tttgctagta tacggattgt tttttagtag taacccaaaa
    12301 gaactatgcg cagctatttt agtgtatatc attggcgttg ctatttatgg tggcctttca
    12361 agcaatatta gtatcttttt caaacaaggc acattgccag tattggcact tatatcaatg
    12421 ctttggtaag tattggtgtt tgggggtttg gagatgtagt cggaggtttg gaggatttga
    12481 gcgatttggg tatggacgtt cataaactca gagtatttca tcctaattat ttcaagcaga
    12541 ggtgacagta gcgttgcctc tgtttcctta tagaaaaatt atttaatgaa gaaaacccat
    12601 atctatttta atttttagct gataaatact ccatctacta aaatggctac tttttccatt
    12661 acatcactcc tttatatcaa aaaaaccatt atttcgaaat ccccatatgg ttgggttcct
    12721 actctcatgg atctgttaaa taaatattat cactatcagt ttattattcc aatattatta
    12781 acaatatatg tagtcgtaaa aggaaagaga acatgagaac ttcggtgttg attggcattg
    12841 cataatgctt ttaaacaaat aatttggtaa caataagaaa cgatttacac aaaggtaatt
    12901 gcatagtaaa attattttat aagacttaaa ttgaattgta caaggagtgg atatcatggt
    12961 accagaagaa aaaggttcta ttactttgtc aaaagaagca gctatcatat ttgcaactgc
    13021 aaaattcaaa ccatttcata atagaattaa aaataaccca caaaaaacaa atccatttct
    13081 taaattacat gaaaacaaaa aaacttaatc actttattta tagcatttct aatctcagaa
    13141 atttaataaa ggacaacgcc ctctttcata attatataag tcaagaaacc aagacatttc
    13201 aaacatcttt aaacccttca atagcacatt ccctaaaaca taaagcaata acaatgaacg
    13261 atcaaaattc cttacgtact tatgatgtgt cagtgaatcg tctacactta attgaacaaa
    13321 ttctatgaga atagatattg ttaaattaag aaagtagtca attttattat gacaaaagaa
    13381 agaagaacat ttagttcaga gtttaagtta caaatggtta gattatataa aaatggtaag
    13441 cttaagaatg aaattatacg aaagtatgat ttaaaaccct caattttctc aaattcgata
    13501 aaacaacacc aaaatactgg atccttcaat catcaagata atttaaaaag tgatgaaaaa
    13561 gagttaataa aattacgcaa agaagttcaa catttaaaaa tggaacatga tgttttaaag
    13621 caaattttag agttgattta gaaactgaac tttttaaaga tacattactt gaagattcta
    13681 atttatctga ttatataaag aaccaacttc caacgatatg gagagactaa gaaatagttt
    13741 aaatttattt aatttcaata aaaactcata ataatattta agtaagttag attgctttta
    13801 gcattaggaa ttcgcttata attagatgtt caatattgac ttttttacac atacatgaac
    13861 tataaaaagc gaataaatag aagatcattg cgaaatttgc taaatacaaa attgatgagt
    13921 tccacctaat aaaaattgtt ggttttatag taacatctat tttcgtacca ttcatgttaa
    13981 tttcagtgct aataatgctg atataaagaa gaataaattg attaggagaa cactatttaa
    14041 tgtctaaaaa tatcaatcaa gcaaatagta aattaaatac gtcaaacaaa aaactaaaac
    14101 aagcttattc aaaatccgat agtaaaaaca tgaaagttat atacatgcct cattggctag
    14161 agttttattc aattactata catagcgatg ttacaagcaa taaaaaaaga tattataaat
    14221 catatagtag aggaacagtg gtatatgtaa aactagggag taatattgga agtgagtttt
    14281 ctggtaatca tttttgtgta atattagata acaaagacaa taaaggaaaa gaaacagtta
    14341 caatagttcc actttcttcc aaaggtaata aaaattattt gaagttaaac gaatcggtgc
    14401 taaatctaac agcaacagat ttaaaaaaac agattatcga tatcagtagt aaagtacaag
    14461 cattagccca aaaatataga gatattgaca ttaaacttag cacagaagat aaaaagcttt
    14521 tgtctaactt aaatcaaaaa gctaatgaac tgtatagagt gataggtgtt tattccaagc
    14581 acaaagggaa agatacatac gttaatatat cagcaattac cacaataagc aaaagacgta
    14641 taagcaaaat aaatgattct gatcctactg gaacgataat aatttcagaa gatgacatga
    14701 atgaaatcga aaagcaatta aagataaagt tcttttctaa ttgaattgta atattacatc
    14761 tgatataatt gatttacaaa ttcccggtaa ccattccggc ttcattctct tgaagaatga
    14821 catattatca gcgttttagc tgaaggtcag atgatacgtc atctggcctc ttttttgata
    14881 taaatgccct ttaagtgaaa tatacaacaa tctgcaatca atattagaca tcacaaaaca
    14941 cacaataaat tacacaatgt cttataagcc acatgtcact attatcgcag ctagctgcgt
    15001 cactcaaaac gaatacggaa cgcgttggtt atttcaatca tattattact ctgcaaatac
    15061 acagccctgt aaccgagtaa atggatagag atttgaacaa atgaaaacaa tcaactaatg
    15121 gaaaggataa aatattatgc acaacaataa tgaattcaac aaaaaactga aagattttat
    15181 aggtagcgat aaacggatgg ttttagtaaa gggttatgtt aacgagtata aattaacaac
    15241 tgttttaaga gctttaaata attccgatta cacaaaaggg acgatacaca ctcgttcaat
    15301 aggcaaccta aaacaaatag tagataatcg aattattcct aaaaatataa ctcaaaacac
    15361 aatcttcaaa ttgagtaatt taaccttaga ggttaacctc tatgaaagaa acacaattta
    15421 tcacggagac tttgctattt actatcctgt agaatctgct ctaataatag aaacagatac
    15481 tcctaggcta attaatcata taaataacaa cccagtccaa aaaatattta taataacaac
    15541 aaatgactgg agctttaata cacttgaatt agaaaaaata gtagacgaaa ctataattta
    15601 tgatttaaaa caagaagatc ctaaacaata tgaaatttta tataaaaata aacacggaaa
    15661 attgccttat tgatgaatta aaacgcaaac ttagaacacc ttaccccttt tccgtcctta
    15721 ttgtaatttt ccaactactc aaattaaaaa ccacccgtgc aaacaggtgg ttttattata
    15781 tataattatt cccactcgat tgtgcttggt ggttttgatg taatgtcata gactacgcgg
    15841 ttgacgtgat ctacttcgtt tacgatacga ctagaaatct tttgtaagac ttcccaatcg
    15901 atgcgtgcga agtcacttgt cataccgtcg atagatgtta ctgcacgaat acctactgtg
    15961 tgatcatacg tacggtagtc tcccataaca cctactgatt gaatgtttgg taacactgtg
    16021 aagtattgcc aaatttctct ttcaagacct tcttctctaa tcacttggcg taaaatcgcg
    16081 tctgattctc taacgatttc tagtttatct tcagtaattt ctccaagtac acgaatacca
    16141 agaccaggtc ctgggaatgg ttgtctccat actaaatgtt ctggaatacc tagttcaata
    16201 cctaatttac gtacttcatc tttaaacaat gtattgattg gttcgattaa ttcgaattcc
    16261 atgtcttctg gtaatccacc aacattgtga tgtgatttga ttgtttgtgc tgtcttagta
    16321 cctgattcga taacgtctgt atatagtgtt ccttgcgcaa ggaagtctac acctttcaat
    16381 tttgatgctt catcatcaaa tacgtataca aattcattac caatgatttt acgtttttgt
    16441 tcaggatcag aaacaccttt taatttattc ataaagcgat ctttcgcatt aacacgaata
    16501 atattcatgt tgaaaccttc accgaattgt tccataacca tgtcgccttc acctttacga
    16561 agtaagccat ggtctacaaa gatacatgtt agttgatcac ctattgcttt atgcaatagt
    16621 acagctacaa cagatgaatc tacgccgcca ctcatcgcac ataatacacg acggtctcct
    16681 acgcgttgac gaatcttttc aatttcgatt tcgataaagt tttccatcgt ccattgtcct
    16741 ttacactcac aaacacgacg tacgaagttg tttaataagt cattaccata ttctgtatga
    16801 cgtacttctg gatggaattg cacaccataa atgcgacgtt tcttatcttc gattgctgca
    16861 tagtctgtgc ttgggctatc agcgataact tcaaagcctt ctggaatttc aataacttta
    16921 tcagaatgac tcatccaaac agtttgttct gctggtaagc cagcgaataa ctcatctgac
    16981 ttcgcattaa tgattgcttt accgtattca cgttcattgg cacgttcaac tttaccacct
    17041 aataatttag tagttaattg catgccgtaa caaataccaa gtactggaat tcctaaatta
    17101 tatatttccg gatcaattgt aaatgaacct tcttcataaa ctgaatttgg accacctgat
    17161 aatatgatac cttttggatt cattttctta atttcttcaa ttgaaatttc atgatcgtgt
    17221 aattcactat aaacgcccat ttcacgaatt cggcgtgtaa ttaattggtt gtattggcta
    17281 ccaaagtcta agacaaggat taactcttgt tcttttgcca tttccatatt tgtcgttctc
    17341 ctttatctta attagaatga gtagttcggt gattctttcg taatttgaat attatgtggg
    17401 tggctttctg ctaaaccagc agggcccata cgtgtgaatt gtgcttcttc gcgtaattct
    17461 cttaaatcgt gtgaaccagt ataacccata ccggcacgca ctccgcccat taattggtaa
    17521 attgtatctt gtaaagcacc tttatatgct gtacgtcctt cgataccttc aggtacaaat
    17581 ttcttaggcg ctttgtcctc ttggaagtaa cggtcgtttg aacctttttc catcgcacct
    17641 aaagagccca taccgcggta tactttatat tgtctacctt ggaaaatttc tgttgcgcct
    17701 gggctttctt cagtacctgc taataagcta cctaacataa ccgcatgtcc accagcagct
    17761 aatgctttaa tgatatctcc tgagaattta ataccaccat cagcaatgat agctttacca
    17821 tgtttgcgtg cttcagttgc acaatcataa attgctgtaa tttgtggtac accaacacct
    17881 gctacaacac gcgtcgtaca aattgaacct gggccaatac caactttaac aatatctgca
    17941 cccgcttcaa ataaatcttt tgttgcttct gcagttgcta cgttaccagc tactaatgtg
    18001 atttctgggt aagtcttctt aatatgtttc acttgatcga taacgccttt agagtgacca
    18061 tgtgctgtat cgataactaa gacatccaca cctgcttcga ctaatttttg agcacgaata
    18121 tcagtgtctt ttgaaatacc gattgctgcg gctacaagta gacgaccatg ttcatctttt
    18181 gctgcattag ggaattcgat aactttttca atatctttaa tagtaataag accttctaga
    18241 cgtccgtctt taactaatgg taacttttca atcttatgtt tttggagaat tttttctgct
    18301 tcttcaagtg ttgtattcac tggagctgta attaaatttt cttgcgtcat tacatctaca
    18361 attttaatcg agaagtcttc aataaaacgt aagtcacggt ttgttaaaat acctactaag
    18421 ttgcgatctt ctttattatc aacaattggt acacctgaaa tacggtattt acccattaat
    18481 gcttctgctt cataaacgct ttcttctggc gttaagaaaa atgggtttga aatgacacca
    18541 ttttctgagc gttttacttt ttgaacttcg tccgcttgtt cttcaacgcc catattttta
    18601 tgaataacac ctaaaccacc ttgacgagcc atagcaatcg ccattttaga ttcagttact
    18661 gtatccatac cagcagaaat aactggaata tttaatttaa ctttgtctga taattgtacg
    18721 cttaaatcaa cgtctttcgg taaaatatca gattgtgctg gaattaataa cacatcatca
    18781 aacgttaatg attcttttgc aaatttactt tcccacatta aaaacagcct ccatttttca
    18841 aattaattag ttatattatt tcacattttc ttctgtttgt ttatacttta ttccattaaa
    18901 aaagaaatta agaataattg cagagattgc gcctaaaaca attccgtttt gagtcaacca
    18961 tgcaaattgt tcacctaaac ctttaaatgc ttgtggtaca gcgcttatac cagtacctaa
    19021 tcctactgat acagcgataa ttaataaatt gttttgattt ttaaaatcga tatgtcctaa
    19081 tatactaaca ccatatgcca ttaccatgcc aaacatagct atcatcgcac cgcctaacac
    19141 aggtagcggt atgatatttg ctaatgcgcc aagcttaggt atacaaccac atataagtaa
    19201 taacacgacc atgccgtata taacattatt tttcttagca ccggataaag aaacaagtcc
    19261 tacattttgc gaataggctg tatacggaaa tgaattgaat atagaaccta acactatcgc
    19321 tagaccttcc gcagtataac ctttacgaaa atcttttctt tctaacttct taccggtaat
    19381 ttcacttaac gcatgataga cacctgtcga ctcaattaaa ctaacgatag ctacaataaa
    19441 gaacactaac gtcgatgtca catcaaagct aaatcctgag aatctaaacg gcactgggat
    19501 gcctaaccaa ccggcatgat tgacttgatt aatatcgacc atcccaagta agccagcacc
    19561 tatcgttcct aaaacgagtc caattaatat ggcaatactc ttaataaatc cagttgtgaa
    19621 tctttgtaaa acaagaataa tgattaatgt cattaaacct aacaaaatgt tcttaacatc
    19681 tccatagtcc tttgcacctt gacctccagc taagtaattc attgctactg gcattaaatt
    19741 gataccaatg atagtaacaa cactacccgt tactactggt ggaaagaatt ttacaagatg
    19801 tgaaaagaaa ggcgcgatga taataactaa tatccctgat aaaaatagcg aaccgtaaag
    19861 tacatctatt cctttcgttt gaccaattaa aatcatgggc gcaacagccg tgaatgtaca
    19921 tccaagaacg attggtaatc ctgttcctgt tactttattg gcttgtaaaa atgtggcaac
    19981 tccacacata aatatatcta ctgtaactaa gtaagcgatt tgttcaggcg taaacttcaa
    20041 acttgtacca acaatgattg gaactaagat ggcacctgcg tacatagcta aaagatgttg
    20101 aacacttagg attaaatttt tcattattct tctcccacca atgtcacttt atttccttct
    20161 agtgaagcaa ccttgcagag agaagaaacc gttaaacctg cttcttctaa acgttgatgc
    20221 ccattttgga aactcttttc aacaacaata ccaataccag ctgtcttagc attcgcttgc
    20281 tgtgcgatat catataaccc taatgaagca tcaccatttg ctaaaaagtc atcgatgatg
    20341 agtacagtat cttcttctga taaaaattct tttgaaacaa tgaccgtact tgttttattt
    20401 ttagtaaatg aatgaataga tgtttcataa taaccatccg tcaaagtgct aggttttgct
    20461 tttttcgcaa ataaacatgg cacatcaaaa tgcagtgcag ccatgattgc tggtgcgata
    20521 ccggaagctt caatggttaa gattttagta atccctttat ctttaaattg ctcgtaaaaa
    20581 gtgcgaccaa cttcattcat taactttgca tcaatttgat gatttaaaaa tccatcgact
    20641 tttaaaatct tctcatcaat gacaatgccg tcttccttta ctttttgtcc tagtaactcc
    20701 actcttaaaa cctcctcaga tttgtgtgaa acattttgct cttaaattgg tgctagatac
    20761 aaaaaaatcc ccaaactaaa taatagtttc agggtttatg agtgaacgaa catgcataac
    20821 gaatttgtca tgcaatcaat gtaaaagaag tttcatcaaa taaactgtga ccatcatata
    20881 aaatgatata aatcacccac catggttaca atttaatggc tgaagctact cctagtattg
    20941 tgttgttact catagtcatg tcgttcaagg caacatggta gaaacttcta aagccatatt
    21001 ctttagatta tatgagttta tgtaaattat ttaacgataa tagcaaattt tcggcatttt
    21061 ttcaataact gcttaggtaa tcttttaatc gttttataaa tgaatagaga cgtttaatgc
    21121 gaatgaagac ttataactta ataatgtaag ggttttattt tataataaga agggtaaaag
    21181 attgaataaa caaattttta aaggggcgac aactatggca gatattactg tagtaaatga
    21241 cactggcgaa ttatacaacg taatcaatca aaagaaatct gaaggttatt tagaatcaga
    21301 gctaacaatt attagtaaaa gtaaattaca cctaaatgac ttacacgact ctgaaatctc
    21361 ccttatctca acaagtggaa catttagtga taaaatgact aaactgttaa caggagaaga
    21421 tggtgaacat gcagttttat cacgctacaa cttagcgcct gatgaactag aaaaatataa
    21481 acaacttatt ttagacgata aaatgttagt tgttggcgtt cgcgatcatt catctcacca
    21541 agaagtacta gaaaataatt cagcttatga agaagttgat attacacatt ttgctgaagc
    21601 atctaaaggt cccaaagctt agttatcaca cttaattcaa aatgagatta ctgtactgtc
    21661 taatttgaga tagtacagtt ttttatacca tttaaaagtt tatgaacagt cttttacaac
    21721 aggcgattat gctataatat tatttaaatt ggggaggtgt tcttatggaa tttaaaacca
    21781 ttcaatctgt caaagatcca cattataacg aagcacttaa actttacgat actaagttaa
    21841 atttatcttt aactgaagat aaacgtatca tcaaacgctc attagaaaac aacaaaactg
    21901 aaaacgatta tgtctttatc gtaggcttag aaaacgatgt tgttgtcagt ctagcaactg
    21961 cacattatga agccacaacc aattctgcat ttttaattta tttattcgca aaagacctac
    22021 ccaaccatga tgagttaatg acacaaacat tacatcaaat tgaacatgaa ctaaacttat
    22081 tatcaaacct tgttcattct agaagtatta actttttaat gatggaagtc cctaaagaac
    22141 ctgcagattt aacacaagat aacggactca ttattgaaca ccgtagacag tttttatacg
    22201 aacatcaatt tgaaagacaa actgaaattg attacattca tccaactaat gatggtcaca
    22261 ctaaaaaagt ggacttattt ttaaagaaaa atattaaact gacaaaggat atctacggta
    22321 caagtgttaa atcaaattat attttgaagt atgtatttgc caatggtatc gcacgtgaaa
    22381 ttatttatgc tttattagaa gaaatggatt tacgtaaacc attttaaaca aacttgaaat
    22441 cactaacaat aacgtttaaa ctatgtatga agcaagactg acgtttcact gcccgtagac
    22501 aaacgtcagt atacataaag tatatgcaga aaggacttta aagtatgtta actaaagaat
    22561 ttgcacaacg cgtagaacta agtgagaagc aagtccgtaa aattgttcaa catttagaag
    22621 aacgtggtta ccaactaagc aagactgaat atcgtggtcg tgaagcaact gatttcaaag
    22681 aagaagatat cgagcttttc aaagacattg ccgacaaagt aaaacaaaca aatagttatg
    22741 atctagcgtt tgatgaatta gaaaaagaaa aagacttcct gcaagtcatt gtcaaaaacg
    22801 atgacaaaaa cttacctact aatcaaaatg ttgctcaatt agtagaagat ttacgtctag
    22861 aaatccagaa aatgcgcgaa gaacgtcacc tacttggtca aatgatgaat caagtacatc
    22921 agcaacaaca agaattaaaa gaacttcaaa atcaacttac aactaaaatc gattcaaata
    22981 gtgaatcctt aaaagccatc caaacattac aagaggctat ccaagaagca caagcatctc
    23041 aagctaaagt attggctgaa tcaacaaata aagttgaaaa taatgctgtt actgaagata
    23101 aagctgacag taaagattcg aaggttgctg gagtgaatac atcaacagac gccaagacgg
    23161 atactaaagc agaaaatgca ggcgatggca ctgctactaa agtagataaa gaggatcaaa
    23221 tttctgcaac tgaagcaatt gaaacagcca gcgttgagca gtctaaaaat gacaaagcag
    23281 ctgagacttc aaataaagaa gctacagtag atgcagaagc gcaacatgat gctgaacaac
    23341 aagtagcaga agcccatgca gaagcttcta aacaagttac ttcgaacgat tcatcggaag
    23401 cgaaagcaga aaatgacagc acagcttcac agtctgaaat ggcagaacct aaacctcaag
    23461 aagagaaaaa aggtttcttc gcacgtttat tcaacctata aacttatgat ttgaacacaa
    23521 gtttaagact cagtaatgta tagctatgat ttaaaaactg atcttttata aacctataga
    23581 actgtatttt attttcaaat ttaatactca attcataaaa cctgaaacaa gctgatatac
    23641 tcctttcaat atctactttg aagagagcat atcaaactaa tttgtttcag gttttttaat
    23701 tattcattat gattaatatt gaattttgaa taaatttttg tagtatgtta cttttaaatg
    23761 ggatggcttc tgtgtattcc taaatagatt catcttataa gcccattcaa tttaaatgtt
    23821 tggttgtgtt agtaactgac gaatcgcaca agagcacact catattcaca tttactacaa
    23881 acgccttttt gctcaaacaa aaatatttgt ttacggtagc ttttgaacta ccacctgcac
    23941 acacgaatcc tcacaagatg ctaattttga ttcgacagaa ttttttacaa cgtgtttgtt
    24001 cgaagcgtct tttcaaagtt gatcatttat tatcttttta ttcaataaat atgaggttag
    24061 caatttttat caaatcgaaa tagtcacatc tcgattcagg aatacatttg ttttctcata
    24121 tcttatctct catgacagta cacgatatat atcttttcca aatgttcatc acttttttcc
    24181 tgttaaaatc atttctatta ttaacgctgt aatgacaact catttaattt attgcattta
    24241 atgccctgct tcattttcaa atatcgactt taatcgacta gctcataaca ctattaacgc
    24301 tattaacgat attaaccctt ttcacctttc tctactaaat tcaaaacgat ttgtttacaa
    24361 attcccttat gccaaaggtc gaatttcata atgtttacat ttaattgcaa atgccctctt
    24421 gaaatatttt aaaattaata ttattcttag catactagtc ggaattatat tcaaaaattt
    24481 gaggagtgac gttcatgaat gcttttctta cactgattaa tattattgta ttggtgattt
    24541 tcatcgtaat attgcacatg atggctcgca aacatatatc ttttgcgaaa cgtgtgttta
    24601 cagctttagg gattggtata gtattcggcg tgttattgca tcttgcctat ggcacacatt
    24661 cgaatattat tacatcaaca agtgactggt tcaacattgt cggacaaggt tacgttgcac
    24721 tgttacaaat gattgtgatg cctttaattt tcatttctat tgtcgctgca ttcactaaaa
    24781 ttcaaatcgg agaaaagttt gctaaaattg gtagtctcat ttttatcttt ttaattggca
    24841 ctgtcaccat tgctgcaatt gttggtgttg tgtatgcctt ggtgtttggt ttagacgcat
    24901 caacaatcaa tcttggtaac gctgaacaag cacgtggaag cgaaattgct aaacaagcga
    24961 aagatttaac agcacatacg ttaccacaac aaattttgga attattgcct aaaaatccat
    25021 tcttagattt cactggacaa cgtgcaactt ccacgattgc tgtcgtgata tttgcttcat
    25081 ttataggatt tgcttattta cgcgtagcac gcaaacaacc tgatcatggt gaattgctta
    25141 agcgtgcgat tgacgctatc tattcactag taatggcgat tgtgacgttc gtcttacgtt
    25201 taacgccata cggtgtatta gcaattatgg ccaacacatt atcgacaagt gactttggcg
    25261 ccatttggac tttaggaaaa ttcttaatcg catcgtacgc tgcattaatt acgatgtata
    25321 tcattcactt aattatttta agcctgttag gcattagccc tattcgctat gttaaaaaga
    25381 ctttggaagt attaatcttt gcattcacat cacgctcaag cgcgggtgcc ttaccactga
    25441 atgtacaaac acaaacaaga cgtctaggcg tgccggaggg tattgccaat ttcgctgcga
    25501 catttggatt gtcgattgga caaaatggtt gtgcaggcat ttatcctgcc atgcttgcaa
    25561 ttatggtcgc acccgtcgct aatgtagaaa ttgatttaca atttattgta acgttaatcg
    25621 ctgtcgtgat tattagttca ttcggcgttg ctggcgtcgg tggcggggct acatttgcat
    25681 ccatattggt gttgtcaacg ttaaacttgc cagtcgcttt agccggcgtg ctcatctccg
    25741 ttgaaccatt gattgacatg ggtagaacag cattaaacgt gaacgattca atgctagctg
    25801 gtacaggtac cgccaagtta actaaacatt gggacaaaga tactttcgaa tcaaatgata
    25861 atgctgcatt aacttcacac taattcattg caagaaatac ccgccccctc tagctatact
    25921 tatcatttta agctagaggg ggcgggttat tttggtatct tgctatcgct gtattaagcc
    25981 tgatttttgc aattgttcta acatatctaa tcttgctttg tttcctagga acatctcaat
    26041 ttgctgcgac catgtttctt tgcgattccc gtttgtacgt tgatcatagt actcgctgat
    26101 tgtctgatcg taatctgcca tttgtgcata ctgagtttcc ttatcagcat gatacttatt
    26161 gtgatggaag acatggtcaa atggtaagcg tggtttggct gcgccatttt catcttctgc
    26221 aggttcccct actgccatac caaataatgg aaagacatag tccggtaagt ctaaaatttc
    26281 tcgaactcgt gcaacatcat ttctcaatga tcctaaaaag acaatgccat accccatatc
    26341 ttcagcagtt accgcaatat tttcggcaac taatgctgca tcgattgcac ctactagcaa
    26401 accttccgtt gaaccatatg cattttccat atcagtttca gcatgttgat caactaaatg
    26461 atgacgataa taatcaataa caaagacgaa taaatagcca ttttctacaa cataaggttg
    26521 tccagaaact tctcgtaaat tttctttaat cttctcatca tcgataccaa taattgagta
    26581 tgcttgtagg aaacttgacg tcgaagcgct ttgtccagct tctaccaatt ttttaacaac
    26641 gtcttcactt aaaggtttat tcttaaattt tctaacagaa tgatgctttt tcacaagatt
    26701 atatatatga tctgacacgt tacccactcc tcgatacttt acagttaatt aaaaatcttc
    26761 atattgcaat tgtacacgtc caaacttttt tatagcaatt atttgcttat atgttaataa
    26821 ttgaactttc aaaaatattg tctcgtactt aaaaatccag tttctcacgc attaccttca
    26881 tccatctcga acaaatattc tcacaccatt atcaataagg ttgacttagt tgagaataca
    26941 aatcttcttt ttttacattt ttttacatta aaatcaagat attaaatata gacacaacct
    27001 tttaaatcat caactacaaa atccattatc aaagcacttt atat
//
LOCUS       DAPIDM010000021        26315 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000021 DAPIDM010000000
VERSION     DAPIDM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.021"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            114..254
                     /locus_tag="RJE42_000809"
     CDS             114..254
                     /locus_tag="RJE42_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876253.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLNRMINVMMCYCVLFLEPCISW
                     DI"
     gene            complement(362..1225)
                     /locus_tag="RJE42_000810"
     CDS             complement(362..1225)
                     /locus_tag="RJE42_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDV5876254.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(1406..2293)
                     /locus_tag="RJE42_000811"
     CDS             complement(1406..2293)
                     /locus_tag="RJE42_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876255.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSSQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYNKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            2359..2454
                     /locus_tag="RJE42_000812"
     CDS             2359..2454
                     /locus_tag="RJE42_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876256.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            2480..4615
                     /locus_tag="RJE42_000813"
     CDS             2480..4615
                     /locus_tag="RJE42_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV5876257.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKTLILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPLRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLISKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESIKEMQQPFSEGDKVKI
                     SNTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            4728..6062
                     /locus_tag="RJE42_000814"
     CDS             4728..6062
                     /locus_tag="RJE42_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDV5876258.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(6256..6645)
                     /locus_tag="RJE42_000815"
     CDS             complement(6256..6645)
                     /locus_tag="RJE42_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876259.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            7002..7310
                     /gene="rpsJ"
                     /locus_tag="RJE42_000816"
     CDS             7002..7310
                     /gene="rpsJ"
                     /locus_tag="RJE42_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV5876260.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            7338..8000
                     /gene="rplC"
                     /locus_tag="RJE42_000817"
     CDS             7338..8000
                     /gene="rplC"
                     /locus_tag="RJE42_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV5876261.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            8027..8650
                     /gene="rplD"
                     /locus_tag="RJE42_000818"
     CDS             8027..8650
                     /gene="rplD"
                     /locus_tag="RJE42_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV5876262.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            8650..8925
                     /gene="rplW"
                     /locus_tag="RJE42_000819"
     CDS             8650..8925
                     /gene="rplW"
                     /locus_tag="RJE42_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV5876263.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            8958..9791
                     /gene="rplB"
                     /locus_tag="RJE42_000820"
     CDS             8958..9791
                     /gene="rplB"
                     /locus_tag="RJE42_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV5876264.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            9858..10136
                     /gene="rpsS"
                     /locus_tag="RJE42_000821"
     CDS             9858..10136
                     /gene="rpsS"
                     /locus_tag="RJE42_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV5876265.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            10165..10518
                     /gene="rplV"
                     /locus_tag="RJE42_000822"
     CDS             10165..10518
                     /gene="rplV"
                     /locus_tag="RJE42_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV5876266.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            10542..11195
                     /gene="rpsC"
                     /locus_tag="RJE42_000823"
     CDS             10542..11195
                     /gene="rpsC"
                     /locus_tag="RJE42_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV5876267.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            11198..11632
                     /gene="rplP"
                     /locus_tag="RJE42_000824"
     CDS             11198..11632
                     /gene="rplP"
                     /locus_tag="RJE42_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV5876268.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            11622..11831
                     /gene="rpmC"
                     /locus_tag="RJE42_000825"
     CDS             11622..11831
                     /gene="rpmC"
                     /locus_tag="RJE42_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV5876269.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            11855..12118
                     /gene="rpsQ"
                     /locus_tag="RJE42_000826"
     CDS             11855..12118
                     /gene="rpsQ"
                     /locus_tag="RJE42_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV5876270.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            12150..12518
                     /gene="rplN"
                     /locus_tag="RJE42_000827"
     CDS             12150..12518
                     /gene="rplN"
                     /locus_tag="RJE42_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV5876271.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            12554..12871
                     /gene="rplX"
                     /locus_tag="RJE42_000828"
     CDS             12554..12871
                     /gene="rplX"
                     /locus_tag="RJE42_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV5876272.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            12898..13437
                     /gene="rplE"
                     /locus_tag="RJE42_000829"
     CDS             12898..13437
                     /gene="rplE"
                     /locus_tag="RJE42_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV5876273.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            13460..13645
                     /locus_tag="RJE42_000830"
     CDS             13460..13645
                     /locus_tag="RJE42_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDV5876274.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            13677..14075
                     /gene="rpsH"
                     /locus_tag="RJE42_000831"
     CDS             13677..14075
                     /gene="rpsH"
                     /locus_tag="RJE42_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV5876275.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            14100..14636
                     /gene="rplF"
                     /locus_tag="RJE42_000832"
     CDS             14100..14636
                     /gene="rplF"
                     /locus_tag="RJE42_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV5876276.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            14667..15026
                     /gene="rplR"
                     /locus_tag="RJE42_000833"
     CDS             14667..15026
                     /gene="rplR"
                     /locus_tag="RJE42_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV5876277.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            15047..15547
                     /gene="rpsE"
                     /locus_tag="RJE42_000834"
     CDS             15047..15547
                     /gene="rpsE"
                     /locus_tag="RJE42_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV5876278.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            15564..15743
                     /gene="rpmD"
                     /locus_tag="RJE42_000835"
     CDS             15564..15743
                     /gene="rpmD"
                     /locus_tag="RJE42_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV5876279.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            15760..16200
                     /gene="rplO"
                     /locus_tag="RJE42_000836"
     CDS             15760..16200
                     /gene="rplO"
                     /locus_tag="RJE42_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV5876280.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            16200..17492
                     /gene="secY"
                     /locus_tag="RJE42_000837"
     CDS             16200..17492
                     /gene="secY"
                     /locus_tag="RJE42_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV5876281.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            17509..18156
                     /locus_tag="RJE42_000838"
     CDS             17509..18156
                     /locus_tag="RJE42_000838"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV5876282.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPTPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            18349..18567
                     /gene="infA"
                     /locus_tag="RJE42_000839"
     CDS             18349..18567
                     /gene="infA"
                     /locus_tag="RJE42_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV5876283.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            18599..18712
                     /gene="rpmJ"
                     /locus_tag="RJE42_000840"
     CDS             18599..18712
                     /gene="rpmJ"
                     /locus_tag="RJE42_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV5876284.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            18735..19100
                     /gene="rpsM"
                     /locus_tag="RJE42_000841"
     CDS             18735..19100
                     /gene="rpsM"
                     /locus_tag="RJE42_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV5876285.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILKEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            19124..19513
                     /gene="rpsK"
                     /locus_tag="RJE42_000842"
     CDS             19124..19513
                     /gene="rpsK"
                     /locus_tag="RJE42_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV5876286.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            19588..20532
                     /locus_tag="RJE42_000843"
     CDS             19588..20532
                     /locus_tag="RJE42_000843"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV5876287.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            20549..20917
                     /gene="rplQ"
                     /locus_tag="RJE42_000844"
     CDS             20549..20917
                     /gene="rplQ"
                     /locus_tag="RJE42_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV5876288.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            21455..22264
                     /locus_tag="RJE42_000845"
     CDS             21455..22264
                     /locus_tag="RJE42_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDV5876289.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIENVKSGEIFYNNQTITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            22261..23121
                     /locus_tag="RJE42_000846"
     CDS             22261..23121
                     /locus_tag="RJE42_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDV5876290.1"
                     /translation="MIIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNVDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKTIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKYLTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            23111..23917
                     /locus_tag="RJE42_000847"
     CDS             23111..23917
                     /locus_tag="RJE42_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV5876291.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGFTI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            23922..24725
                     /gene="truA"
                     /locus_tag="RJE42_000848"
     CDS             23922..24725
                     /gene="truA"
                     /locus_tag="RJE42_000848"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV5876292.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            24965..25402
                     /gene="rplM"
                     /locus_tag="RJE42_000849"
     CDS             24965..25402
                     /gene="rplM"
                     /locus_tag="RJE42_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV5876293.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            25416..25814
                     /gene="rpsI"
                     /locus_tag="RJE42_000850"
     CDS             25416..25814
                     /gene="rpsI"
                     /locus_tag="RJE42_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV5876294.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            26152..>26315
                     /locus_tag="RJE42_000851"
     CDS             26152..>26315
                     /locus_tag="RJE42_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876295.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVLILFRFYIKTDNFSVYLMIIFL
                     MCFGTASIIY"
BASE COUNT         9443 a         4173 c         5049 g         7650 t
ORIGIN      
        1 agaagctgac gaaaagtcag cctacaataa tgtgcaagtt ggggtgggac gacgaaatat
       61 attttgcgaa aatatcattt ctgtcccact ccctatgcag ctatccactt catatgtatg
      121 gcgcatacct gataaacgaa tatcatcaag actttaaaag tatcgctaat tttaaagtct
      181 taaaccgcat gattaatgtc atgatgtgtt attgcgtttt gtttttagag ccatgtattt
      241 cctgggacat atagaggaac attcaacgta aaattcccga aaattaattt taatcattct
      301 ataagtgaat ggttattttc agaagcgata acatttttac gttacatgag tatttaactt
      361 tctatttcaa attacctaga attatagcag ctatcacgat aataataata cctgcccaaa
      421 taccagtcat ctgacgacga tcttttcttt ctcctaaaat gaaaataccg cctaatgttg
      481 aaacgataac aagtaactgt gataatgaga aacttgtagc tacaccaact tttggttgag
      541 aatagaacat gaacaagtta ccaattcccc aaatcacacc tggcaataga tttagtgctg
      601 ttgatttaag tgatgtttta tgattcatgg ataggataaa gccaccaatt gccataccga
      661 cagattggaa gaacaatgca tcagttccac caacaccaaa gatgtcacca agtacaacga
      721 aacctacata tccaactgta gatacaatta aaatacccat tgctttttta aattcaggat
      781 tatctgattg acgttcattt ttagctttaa gtgaagtaag tgctacacca gtaactaata
      841 atatcatggc gattaaacca aagataattt gagtcattga agaccattca cctaaaaaga
      901 taacgctgaa taatgttgta ccaactaatt gcatacctgt agaaactggc atagtatttg
      961 atacaccgat taaactaata gatttaagct gattcgcttg tccaaacgcc cataatgcac
     1021 cagaaataag accgacaata attacagtag gattattgaa ttttgcgaaa ccagtaataa
     1081 gtaaacctaa tccaacaatg agtgcgccta gcgtcgtacc acgaatttgg ttgtaaggtc
     1141 caccgccgac gaacacatta ataagaacga cacttcccca gaataaagca ggtaaaagtg
     1201 cgattaagaa atcaagaaat tgcaagacta ctcactctcc tatactattt atgcaagtgc
     1261 tccccttaat tgacacttgc atgttattgt aaagcattct gccctaatag taaaaaacaa
     1321 ctacttataa ttttcaatat ttacaaaaag tagaaaaacc cacaatacat gctaatgatg
     1381 tactgtgggg tatttgcttg gtattttatt cagggattgt ttgatctttt acgttacgtg
     1441 attcgaattt gaatttaatg tactcatagt tgatatggaa acctagacca ttaaatcgtt
     1501 cgatgagttt attattttta ttacgaattt taaaataaac ttttctaata tcaaagttat
     1561 caaaagcata ttgcaatgca tacgcaagca actcgaaagc gatacctttc aaacggtaat
     1621 ctacatgtga actgaagtat ttaatctcgg cgattgactg ttgtgaatca atttcaaggt
     1681 ataaataacc tttaagtaaa ccttcgctaa caaataaaaa caaatgatga tggtcgtcta
     1741 aactatcaat gatatcttgt ggtgactggg cctgatattt aaatgtagtt gtgtgtaatt
     1801 tgctgaaagc ccgataaaac cctttgttat aaggaatgat atttggttgg tgcatatctt
     1861 cttctaatct tgtacgggct tctatgtaat agtcagtgaa gttataactt gcttgaataa
     1921 ctttcattaa tggtttgtat tgttgaatgc cttcttcaaa agaaaagtta aagacggcat
     1981 cgtcaggttg gctcgaagtc attgctttaa acagcgtttc aaacgtatcg acagataata
     2041 cataattgtc agccacgaaa ggacctatga ctttgaattt atttttatcg tatgcaaaag
     2101 acaatatgat tgctttgatt gaatcgtttt ctttaatcga aaatacacca ggtgattcaa
     2161 tcgcatcggt tattaataca tctatttcat tgtactgttg aggaagttta tataagtatg
     2221 atgctgattc ataattcgaa tgttcaatga acgattttat ttgtgtaata tcatataact
     2281 gaactatgct catgtcatta cctccgtact ttttgttact tttattatat agtatttcaa
     2341 ctgaaatgaa agttaatagt gatattaaca tgttacaata catttaacac atcatttgca
     2401 atcattgaat ttaaatcaaa gattagtgga atagatgaag cgcgttcgaa ataaaagaac
     2461 gtatgagaaa ggataaatta tgaaatcttt aatattagct gaaaaaccat cagtcgcaag
     2521 agatattgct gatgctttac aaataaatca aaagcgtaat ggttactttg aaaataacca
     2581 atatattgtc acgtgggcgt taggtcatct agtgacaaat gcgacacctg aacaatacga
     2641 taaaaattta aaggaatggc gattagaaga ccttccaatt atacctaaat atatgaaaac
     2701 ggttgttatt ggtaaaacaa gcaaacaatt taaaacagta aaaacgttaa ttttagataa
     2761 taaagtgaaa gatattatta ttgcaacaga tgctggacga gaaggtgaac tagttgcaag
     2821 attgattttg gataaagttg gtaacaaaaa gccactccgt cgattatgga ttagctcagt
     2881 tactaaaaaa gctattcaac aagggtttaa aaatttaaaa gacggtcgcc aatataacga
     2941 tttgtattat gcagcgttag cgagaagtga ggcggattgg attgttggga ttaatgcaac
     3001 gcgtgcacta acaacaaagt atgatgcaca gctatccctg ggacgtgttc agacaccaac
     3061 gattcaatta gtaaatacac gacaacaaga gattaatcag ttcaaaccac aacaatacta
     3121 tacattatca ttaacggtaa aagggtttga ttttcagcta gaatcaaatc agcgatatac
     3181 caataaagaa actttagaac agatagttaa taatttgaaa aatgtcgatg gtaagattaa
     3241 atctgttgct actaaacata agaagtcgta tccgcaatca ctgtacaatt taacagattt
     3301 acaacaagat atgtatagac gttataaaat tggacctaaa gaaacattga atacacttca
     3361 aagtttatat gagagacata aagtcgtaac gtatccaaga acagattcaa actatttaac
     3421 aactgatatg gtagatacta tgaaagaacg tattcaggcg acgatggcaa caacatataa
     3481 agaccaagca cgcccattaa tctctaaaac attttcatca aaaatgtcga tatttaataa
     3541 tcaaaaagta tctgatcacc atgcaattat tcctacagaa gtgagacctg tcatgtcaga
     3601 cttaagtaat agagaattaa agttatacga tatgattgtc gagcgttttt tagaagcttt
     3661 aatgcctcca cacgagtatg acgcgataac tgtaacttta gaggttgcag ggcacacatt
     3721 tgttttgaaa gagaatgtaa caactgtatt aggctttaaa tctattagac aaggtgaatc
     3781 tattaaagag atgcaacagc ctttttcaga aggcgataaa gtgaagattt caaatacaaa
     3841 cattagagaa catgaaacaa cacctccaga atattttaat gaaggttcgt tattaaaagc
     3901 gatggagaac cctcagaact ttattcaatt gaaggataaa aaatatgcgc aaactttaaa
     3961 acaaacaggt ggtatcggca cagttgcaac aagggcagac attatcgata aattatttaa
     4021 tatgaatgcc attgaatcaa gagacggtaa aattaaagta acgtcaaaag gtaaacaaat
     4081 attagaatta gcaccagaag aattaacgtc gccactttta actgcacaat gggaagaaaa
     4141 actactttta attgaacgtg gtaaatatca ggcgaaaaca ttcattaatg aaatgaaaga
     4201 ttttacgaaa gatgttgtaa atgggattaa aaatagtgat cgtaaatata aacacgataa
     4261 tttaacaacc actgaatgcc caacgtgtgg taaattcatg attaaagtta aaactaaaaa
     4321 tggtcagatg cttgtgtgcc aagatccatc ttgtaagacg aaaaagaatg tacagcgcaa
     4381 aacaaatgca agatgtccaa actgtaaaaa gaaattaacg ttgtttggta aaggaaaaga
     4441 agcggtatat cgttgtgttt gtggacattc tgaaacgcaa gcacatatgg atcagcgtat
     4501 gaagtctaaa tcctctggta aagtatctcg taaagaaatg aaaaagtata tgaataaaaa
     4561 tgaaggttta gacaataatc cgtttaaaga tgcattaaag aacttgaatt tatagataaa
     4621 atcgaacaaa gttgaatcag aaaatcgaaa agttcgcttt tggtattgtt ttttattaag
     4681 aatgatatta aactattaag gtattttaaa aaaaggagca tccattcgtg aaaaactatt
     4741 tccagttcga taaatatgga acaaacttta aaagagaaat attaggcggt atcacaactt
     4801 tcttatctat ggcctatatt ttagcagtta acccgcaagt tttaagttta gcaggtgtta
     4861 aaggcgtatc agaagatatg aaaatggacc aaggtgccat ttttgtagcg actgcattag
     4921 cagcatttgt aggctcgcta ttcatggggc taatagctaa atatccaatt gcgttagcac
     4981 caggtatggg attaaatgca ttctttgcat ttactgttgt gttaaccatg ggtattcctt
     5041 ggcaagttgg tttaacaggt gttttattct caggtatatt ctttgcgata ttaaccgtca
     5101 cagggtttag ggaagttatt attaatgcaa ttccttatca aatgaagatg gctgtttcag
     5161 caggtatagg gttattcatt acatttgtag gtttacaaag ttcgggtatt atagttaaaa
     5221 atgaatctac attagtaaca ttgggacatt taactgatcc accagtactt ttagcgatat
     5281 tcggtatcgt tattacagtt attttatatg caattaaatt gccaggttct atctttatcg
     5341 gtatgatcat cacagcaatc gttggtatgt tcactggatt gattcaaatg ccatctggta
     5401 ttgtaggtaa gattccaagt attgaaccaa cgttcggtgc tgcatttgaa gcgtttaaag
     5461 acccaagtca attattaacg atacagttct taattgttat tttaacgttc ttatttattg
     5521 atttctttga tacagcaggt acattagtag ccgttgctac tcaagcaggt attatgaaag
     5581 acaataaatt accaagagct ggtagagcat tgttctcaga ttctttagct acgattgtag
     5641 gatcagtttt tggtacaact acaacaactt cttatatcga atcaacttca ggtgttgctg
     5701 taggtgctag aacaggattt gctagtatcg taacaggttg ttgtttccta ttagcgctat
     5761 tctttagtcc gttaatcgct gttgtaacaa gtgcagttac gacaccagct ttagtggtgg
     5821 taggtgtatt aatggctgca aactttgctg aaataaactg gaaaagtttt gaagttgcag
     5881 tgccagcatt cattacaatc attatgatgc ctttatcata ttcaattgca actggtattg
     5941 cgtgtggatt tattttctat ccaattacaa tgcttatctc taaaaaacat aaagaagtac
     6001 atccgataat gtatgtatta atggtactat tcattttgta cttcatattt gttcatggtt
     6061 aaatccagac acaaaaaacg actaccaatg attggtagtc gtttttttct ttgaagtaag
     6121 gacaaaataa gaagcgaaat aacataaagc aatgttatga atgtctttaa atatccgata
     6181 taaatatact tcttttaaaa ttgttgaacg tgtttcttct atattatata tacctaatta
     6241 aaaaggtttt aagaattatt ctctatgtcc atctttgtat tgtgtgtaaa tgacacgagc
     6301 atttttatta ggtatatagg ttggcgctga taatacaact aggacaaata gaatcattag
     6361 gaaaaataaa atccaatgaa tcaaaattcc taagaagggg ataatagcta aaaaactacc
     6421 tactatacca aatattggaa taatcgctat aggtctaatt gcattttgtc tattcactaa
     6481 taatataata gtaagaatta gataaagtaa tgcattaaag agtaaaggct gccatccaaa
     6541 acttaaaata aacgttgcac ctaaaaaagg aatgccatag aaaaactcac aaagtattaa
     6601 aaagatacct ataagtgcta aagataattt tggtccattt ttcatattca ttcaccaccg
     6661 ttcttatgac tcattatata gaataatttt caatgataat aataaaatga ttcaaggtac
     6721 gaatttacaa taaagaagga aataaaaaat atctcgaaaa tagttgaact gactaagtat
     6781 ataaagtaaa atataaaagt atgtgttaga cagatagaaa aattatgtga aaagccttga
     6841 tttatcgatg tttttcgtgt aatataacta acgttggact ttaagaaaag cgatgaagcg
     6901 aaaggttact gacacacccg gccgctttgc catggcgctg tgtaagatag ttttcgtgga
     6961 gaagtctatc actaaatgta gacgaataag gagggaaaat tatggcaaaa caaaaaatca
     7021 gaatcagatt aaaagcttat gatcaccgcg tgattgatca atcagcagag aagattgtag
     7081 aaacagcgaa acgttctggt gcagatgttt ctggaccaat tccgttacca actgagaaat
     7141 cagtttacac aatcatccgt gccgtgcata agtataaaga ttcacgtgaa caattcgaac
     7201 aacgtacaca taaacgttta atcgatattg taaacccaac accaaaaaca gttgacgctt
     7261 taatgggctt aaacttacca tctggtgtag acatcgaaat caaattataa tagacaattt
     7321 taggaggtgg actttcgatg accaaaggaa tcttaggaag aaaaattggg atgacacaag
     7381 tattcggaga aaacggtgaa ttaatccctg taacagtagt agaagctaaa gaaaatgttg
     7441 tattacaaaa gaaaactgta gaagttgatg gatacaacgc aatccaagtt ggatttgaag
     7501 acaaaaaagc atacaaaaaa gatgcaaaat ctaataaata tgctaataaa ccagctgaag
     7561 gtcacgctaa aaaagctgac gcagcaccta agcgcttcat tcgtgaattc cgcaatgtag
     7621 acgtggatgc ttacgaagta ggtcaagaag tctcagtaga tacttttgta gctggcgacg
     7681 ttattgacgt aacaggcgta tcaaaaggta aaggtttcca aggtgcaatt aaacgccatg
     7741 gacaatctcg tggacctatg tcacacggtt ctcatttcca cagagcgcca ggttctgtag
     7801 gtatggcttc agatgcttct agagtattta aaggccaaaa aatgccagga cgtatgggtg
     7861 gaaacactgt aactgttcaa aacttagaag tagttcaagt tgacacagaa aacaaagtta
     7921 tcttagtaaa aggtaacgta cctggaccta aaaaaggttt agtagaaatc agaacttcaa
     7981 ttaaaaaagg taataaataa taagaagtga aaggaggaaa tgcataatgg ctaattatga
     8041 tgttttaaaa ttagacggaa ctaaatcagg ttcaatcgaa ttaagcgatg cagtattcgg
     8101 tattgagcca aataatagcg ttttattcga agctattaat ttacaacgtg cttcattacg
     8161 tcaaggtaca catgctgtta agaatcgttc agcagtaagc ggtggcggac gtaaaccatg
     8221 gaagcaaaaa ggaacaggtc gtgctcgtca aggtacaatc cgtgctccac aatggcgtgg
     8281 cggtggtatc gtattcggac caactccaag aagttatgca tacaaaatgc ctaagaaaat
     8341 gcgtcgttta gctttacgct cagcattatc tttcaaagct caagagaatg gcttaactgt
     8401 agttgacgca ttcaacttcg aagctccaaa aactaaagaa ttcaaaaatg tattatctac
     8461 attagaacaa cctaaaaaag tattagtagt tactgaaaac gaagatgtaa atgttgaatt
     8521 atcagcacgc aacatccctg gcgttcaagt gacaactgct caaggtttaa atgttttaga
     8581 tatcactaat gctgacagct tagtaattac tgaagctgct gctaaaaaag ttgaggaggt
     8641 gctcggataa tggaagcaag agatattctt aagcgccccg taatcactga gaaatcttct
     8701 gaagcaatgg ctgaagacaa atacactttc gacgttgata ctcgtgttaa caaaacacaa
     8761 gtgaaaatgg cagttgaaga aatcttcaac gtaaaagttg caagtgttaa tatcatgaat
     8821 tacaaaccta agaaaaaacg tatgggccgt taccaaggct atacaaacaa aagaagaaaa
     8881 gcgattgtaa ctcttaaaga aggatcaatc gacttattta actaataaac caattaccat
     8941 taaggaggta agcgacaatg gctattaaaa agtataagcc aataacaaat ggtcgtcgta
     9001 atatgacttc gttagatttc gcagaaatca cgaaaactac acctgaaaag tcattattaa
     9061 aaccgctacc gaaaaaagcg ggacgtaaca accaaggtaa attgactgta agacaccatg
     9121 gtggtggaca caaacgtcaa taccgtgtta tcgatttcaa acgtaacaaa gatggtatca
     9181 atgcaaaagt tgattctatt caatatgatc caaaccgctc agcaaacatc gctttagttg
     9241 tatatgcaga cggtgaaaaa cgatatatca ttgctcctaa aggattagaa gtaggtcaaa
     9301 tcgttgaaag tggtgctgaa gctgacatca aagttggtaa cgcattacca ttacaaaaca
     9361 ttccagttgg tacagtagta cacaacatcg agcttaaacc tggtaaaggt ggacaaatcg
     9421 ctcgttcagc tggtgcaagt gctcaagtac ttggtaaaga aggtaaatac gtattaatca
     9481 gattaagatc tggtgaagtt cgtatgatct tatctacttg ccgtgctaca atcggtcaag
     9541 ttggtaacct acaacacgaa ttagttaacg ttggtaaagc cggacgttca agatggaaag
     9601 gtatccgtcc aacagttcgt ggttctgtaa tgaaccctaa cgatcaccca cacggtggtg
     9661 gtgaaggtcg tgctcctatc ggtagaccat ctccaatgtc accatggggt aaacctacgc
     9721 ttggtaagaa aactcgtcgt ggtaaaaaat catcagacaa acttatcgtt cgtggacgta
     9781 agaaaaaata atataatcaa cttatttggg tgtgcggctt aaagctgcac gcacataata
     9841 agaagggagg cgcccaaatg gctcgtagta ttaaaaaagg acctttcgtc gatgagcatt
     9901 taatgaaaaa agttgaagct caagaaggaa gcgaaaagaa acaagtaatc aaaacatggt
     9961 cacgtcgttc tacaattttc cctaatttca tcggacatac ttttgcagta tacgacggac
    10021 gtaaacacgt acctgtatat gtaactgaag atatggtagg tcataaatta ggtgagtttg
    10081 ctcctactcg tacattcaaa ggacacgttg cagacgacaa gaaaacaaga agataatatc
    10141 tattaagtag aggaggacat cctaatggaa gcaaaagcgg ttgctagaac aataagaatc
    10201 gcacctcgta aagtaagact agttcttgac ttaatcagag gtaaaaatgc tgctgaagct
    10261 attgcaattt taaaattaac aaacaaagct tcatcaccag taattgaaaa agtattaatg
    10321 tccgctttag ctaatgctga acataactat gacatgaaca cagatgaatt agtagttaaa
    10381 gaagcatatg ctaacgaagg accaacatta aaacgtttcc gtccacgtgc gcaaggtcgt
    10441 gcaagtgcga ttaacaaacg tacaagccac attacaatcg tcgtaagtga cggtaaagaa
    10501 gaagctaaag aagcttaatt aacttttaag gagggaatac tgtgggtcaa aaaattaatc
    10561 caatcggact tcgtgttggt attatccgtg attgggaagc taaatggtat gctgaaaaag
    10621 acttcgcttc acttttacac gaagatttaa aaatccgtaa atttattgat aatgaattaa
    10681 aagaagcatc agtttctcac gtagagattg aacgtgctgc aaaccgtatc aacattgcaa
    10741 ttcatactgg taaacctggt atggtaattg gtaaaggcgg ttcagaaatc gaaaaattac
    10801 gcaacaaatt aaatgcgtta actgataaaa aagtacacat caacgtaatt gaaatcaaaa
    10861 aagttgatct tgacgctcgt ttagtagctg aaaacatcgc acgtcaatta gaaaaccgtg
    10921 cttcattccg tcgtgtacaa aaacaagcaa tcactagagc tatgaaactt ggtgctaaag
    10981 gtatcaaaac tcaagtatct ggtcgtttag gcggagctga tatcgctcgt gctgaacaat
    11041 attcagaagg aactgttcca cttcatacgt tacgtgctga catcgattat gcacacgctg
    11101 aagctgacac tacttacggt aaattaggcg ttaaagtatg gatctatcgt ggagaagttc
    11161 ttcctactaa gaacactagt ggaggaggaa aataataatg ttactaccaa aacgtgtaaa
    11221 atatcgtcgt caacatcgtc ctaaaacaac tggtcgttct aaaggcggta actacgtaac
    11281 atttggtgag tttggtttac aagctacaac aacgtcttgg atcacatctc gtcaaatcga
    11341 atctgctcgt atagcaatga cacgttacat gaaacgtggc gggaaagttt ggattaaaat
    11401 cttcccacat acaccatata ctaaaaaacc tttagaagta cgtatgggtg ctggtaaagg
    11461 tgcggttgaa ggctggatcg cagttgttaa accaggtaga attttattcg aagttgctgg
    11521 cgtttctgaa gaagttgcgc gtgaagcact acgtttagca agtcacaaac ttccagtaaa
    11581 aactaagttt gtaaaacgtg aggaattggg tggtgaaaca aatgaaagct aaggaaatta
    11641 gagacttaac cacttcagaa atcgaagaac aaatcaaatc ttcaaaagaa gagcttttta
    11701 acctacgctt tcagttagct acaggtcaat tagaagaaac tgcacgtatt cgtacagtaa
    11761 gaaaaacgat tgcacgtcta aaaactgttg ctcgtgaaag agaaattgaa caaagtaagg
    11821 ctaatcaata attaagtaag aggaggttac aaaagtgagc gaaagaaacg atcgtaaagt
    11881 ttatgtaggt aaagttgttt cagacaaaat ggacaagact attacagtac ttgttgaaac
    11941 ttacaaaaca cacaaattat acggtaaacg agtgaaatac tctaaaaaat acaaaactca
    12001 tgatgaaaac aattcagcta aattaggaga cattgttaaa attcaagaaa ctcgtccttt
    12061 atcagcaaca aaacgttttc gtttagtaga gattgttgaa gagtcagtaa ttatttaata
    12121 caagtttaga gataagggag gtttaactaa tgatccaaca agaaacacgc ttgaaagtag
    12181 cagacaactc tggtgctcgt gaagttctta caatcaaagt attaggtgga tctggtcgta
    12241 aaacagcaaa catcggcgat gttatcgtat gtactgttaa aaatgcaaca ccaggtggcg
    12301 ttgttaaaaa aggtgacgtt gtcaaagctg taatcgtacg tactaagtca ggtgttcgtc
    12361 gtaatgacgg ttcatatatc aaatttgatg aaaatgcatg tgttatcatc cgtgatgaca
    12421 aaggcccacg tggtactcgt atcttcggac ctgttgctcg tgaattacgt gaaggtaact
    12481 tcatgaaaat cgtatcatta gcaccagaag tactttaatt aataacaacc aatacaaata
    12541 aggaggtgcc cacatgcata tcaaaaaagg tgacaacgtt aaagttatcg caggtaaaga
    12601 caaaggtaaa gaaggtaaag taatcgctac tctacctaaa aaagaccgtg tcgttgtgga
    12661 aggtgttaac attatgaaaa aacaccaaaa accaactcaa ttaaatcctg aaggtggaat
    12721 cttagaaaca gaggcagcaa tccatgtttc taatgtacaa ttattggacc ctaaaacaaa
    12781 cgaaccaact cgtgtaggtt acaaatttgt tgatggtaaa aaagttcgta tcgctaaaaa
    12841 atctggcgaa gaaattaaat ctaataatta ataacaagtt gaaaggagga tcccactttg
    12901 aaccgtttaa aagaaaagtt taacactgaa gttactgaaa acttaatgaa aaaattcaat
    12961 tatagttcag taatggaagt accaaaaata gataaaatcg ttgtgaacat gggtgtaggt
    13021 gacgcagtac aaaattctaa agtattagac aatgctgttg aagaattaga attgatcact
    13081 ggtcaaaaac cattagtaac taaagctaaa aaatcaatcg cgactttccg tttacgtgaa
    13141 ggtatgccaa tcggtgcgaa agtaacactt cgcggtgaaa gaatgtatga attcttagac
    13201 aaattaattt cagtatcatt accacgtgta cgtgacttcc aaggtgtttc taaaaaagca
    13261 tttgacggac gcggtaacta cactttaggt gttaaagaac aattaatttt cccagaaatc
    13321 gactatgata aagtaagtaa agttagagga atggatattg ttatcgtaac gactgctaac
    13381 actgatgaag aagctcgtga attgttagct aacttcggta tgccattccg taaataatta
    13441 tttaaaggag gctaattaag tggctaaaac ttcaatggtt gctaagcaac aaaaaaaaca
    13501 aaaatatgca gttcgtgaat acactcgttg tgaacgttgt ggccgtccac attctgtata
    13561 tcgtaaattt aaattatgcc gtatttgttt ccgtgaatta gcttacaaag gccaaatccc
    13621 tggcgttcgt aaagctagct ggtaataaaa aagagtctga aaggaggcaa caatcaatga
    13681 caatgacaga tccaatcgca gatatgctta ctcgtgtaag aaacgcaaac atggtgcgtc
    13741 acgagaagtt agaattacct gcatcaaata ttaaaaaaga aattgctgaa atcttaaaga
    13801 gtgaaggttt cattaaaaat gttgaatacg tagaagatga taaacaaggt gtacttcgtt
    13861 tattcttaaa atatggtcaa aacgatgagc gtgttatcac aggattaaaa cgtatttcaa
    13921 aaccaggttt acgtgtttat gcaaaagcta gcgaaatgcc taaagtatta aatggtttag
    13981 gtattgcatt agtatcaact tctgaaggtg taatcactga caaagaagca agaaaacgta
    14041 atgttggcgg agaaattatc gcatacgttt ggtaataaaa aataaggagg tgccataaca
    14101 tgagtcgtgt tggtaagaaa attattgaca tccctagtga cgtaacagta acttttgatg
    14161 gaaatcatgt aactgttaaa ggtcctaaag gtgaattatc aagaacttta aatgaaagaa
    14221 tgacattcaa acaagaagaa aacacaattg aagttgtaag accatctgat tctaaagaag
    14281 atagaacaaa ccatggtaca actcgtgctt tattaaataa tatggtacaa ggtgtttctc
    14341 aaggatacgt aaaagtactt gaacttgttg gtgtaggtta ccgtgctcaa atgcaaggta
    14401 aagacttaat ccttaacgtt ggttattctc acccagtaga aattaaagct gaagaaaaca
    14461 ttactttctc agttgagaaa aacacagtcg ttaaagttga aggtatttca aaagaacaag
    14521 ttggagcatt agcatctaac atccgttcag taagacctcc agagccttac aaaggtaaag
    14581 gtattcgtta ccaaggtgaa tacgttcgcc gtaaagaagg taaaactggt aaataataga
    14641 taaactctaa agaaaggagt attgaaatga tcagtaaaat tgataaaaat aaagtgcgtt
    14701 taaaaagaca tgctcgtgtt cgtactaact tatcaggtac agctgaaaag ccacgtttaa
    14761 acgtatatcg ttcaaacaag catatctacg ctcaaattat tgatgataat aaaggcgtaa
    14821 cattagctca agcttcttca aaagacagcg acattgctac tacagcaact aaagttgaat
    14881 tagcaactaa agtcggtgaa gcaattgcta aaaaagctgc tgacaaaggc attaaagaaa
    14941 tcgtatttga ccgtggagga tatttatatc acggacgtgt taaagcatta gctgaagcag
    15001 caagagaaag cggattagaa ttttaattta aaggagggac aaatacatgg ctcgtagaga
    15061 agaagagacg aaagaatttg aagaacgcgt tgttacaatc aaccgtgtag caaaagttgt
    15121 aaaaggtggt cgtcgtttcc gtttcactgc attagttgta gttggagaca aaaatggtcg
    15181 tgtaggtttc ggtactggta aagctcaaga ggtaccagaa gcaatcaaaa aagctgttga
    15241 agcagctaaa aaagatttag tagttgttcc acgtgttgaa ggtacaactc cacacacaat
    15301 tactggccgt tacggttcag gaagcgtatt tatgaaaccg gctgcacctg gtacaggagt
    15361 tatcgctggt ggtcctgttc gtgccgtact tgaattagca ggtatcactg atatcttaag
    15421 taaatcatta ggatcaaaca caccaatcaa catggttcgt gctacaatcg atggtttaca
    15481 aaaccttaaa aatgctgaag atgttgcgaa attacgtggc aaaacagtag aagaattata
    15541 caattaagga gggaaaacta gttatggcta aattacaaat taccctcact cgtagtgtta
    15601 ttggtcgtcc tgaaacacaa cgtaaaactg ttgaagcttt aggtcttaaa aagactaaca
    15661 gttcagtagt tgttgaagat aaccctgcta ttcgtgggca aatcaacaaa gttaagcact
    15721 tagtaacagt agaagaaaaa taatcaagga ggtgccgaaa tgaaattaca tgagttaaaa
    15781 ccggcagaag gttcacgtaa agaacgcaat cgtgttggac gtggtgttgc gacaggtaac
    15841 ggtaaaacaa gtggtcgcgg acacaaaggt caaaaagctc gttcaggcgg tggtgtaaga
    15901 ccaggatttg aaggtggtca attaccatta ttccgtcgtt taccaaaacg tggttttact
    15961 aacataaatc gtaaagaata tgctattgtt aacttagacc aacttaataa atttgaagat
    16021 ggtactgaag taactccagc tttattagta gaatctggtg ttgttaagaa tgaaaaatct
    16081 ggtatcaaaa tactaggtaa tggttcactt gataagaaat tgacagtgaa agctcataaa
    16141 ttctcagctt cagcagcaga agctattgat gctaaaggtg gagcacacga ggtgatctaa
    16201 tgattcaaac ccttgtgaac ttctttagaa caaaagaggt tcgtaacaag attttcttca
    16261 cactagcaat gttagtaatt tttaaaatag ggacttatat accagctcca ggagtaaatc
    16321 ctgcagcttt tgataatccc caaggttctc aaggtgccac tgagttatta aatacttttg
    16381 gtggcggagc cttgaaacga ttttctattt ttgcaatggg tattgtaccc tacatcactg
    16441 catctatcgt aatgcaacta ttacaaatgg atattgtccc taaattctca gaatgggcaa
    16501 aacaaggtga agtaggaaga agaaagttaa ataacgttac tcgttattta gcaatttctt
    16561 tagcatttat ccaatctata ggtatggcat tccaatttaa taattatctc aaaggtgcac
    16621 tgattattaa tcagtcaatt atgagttatt tattaattgc actagttttg acagcaggaa
    16681 ctgctttctt aatatggctt ggtgatcaaa tcactcagtt cggtgttggt aatggtattt
    16741 ctattatcat attcgcaggt attttatcaa cgttaccagc cagtctaatt caatttggac
    16801 aaactgcttt cgttgggcaa gaagatacgt cattagcatg gttgaaagta ttaggactat
    16861 tagtgtcact gatactatta actgtaggtg ctatttatgt tcttgaagcc gttcgtaaaa
    16921 ttccaattca atatgctaag aaacaaactg ctcaaagatt aggttcacaa gcaacgtatc
    16981 tacctctaaa agtgaactca gctggtgtta ttcctgtaat ctttgcaatg gcgttcttct
    17041 tactgcctag aacattaacg ttgttctatc cagataaaga atgggcacaa aacattgcga
    17101 atgctgcaaa tccttcaagt aatgttggta tggttgtata tattgtactg atcatactat
    17161 tcacatactt ctatgcattt gtacaagtta atccggaaaa aatggctgat aaccttaaga
    17221 aacaaggtag ctatgttcca ggtattagac ctggtgaaca aaccaaaaaa tatattacta
    17281 aagttttata tcgtttaact tttgtaggtt ctatcttctt agccgttatt tcaattctac
    17341 cgatattagc aactaagttt atgggattac cacaatcaat tcagattggt ggtacaagtt
    17401 tacttatcgt tattggtgta gcgattgaaa caatgaaatc tttagaagct caagtatctc
    17461 aaaaagaata taaaggcttt ggtggtagat aattttagga gggcacttat gaatatcatt
    17521 ttgatgggtt tacctggcgc aggtaaagga actcaagcaa gtgaaattgt caagaaattc
    17581 ccaacacccc acatttcaac tggtgacatg ttcagaaaag ctataaaaga agaaactgaa
    17641 ttaggtaaag aagctaagtc ttatatggac cgtggcgaat tagttcctga tgaagtgact
    17701 gtaggtatcg ttaaggaaag aatttctgaa gacgatgcaa aaaaaggctt tttattagat
    17761 ggcttcccaa gaacaatcga gcaagctgag gcattaaata atattatgtc tgagcttgac
    17821 agaaacattg atgctgtcat caatatcgaa gttccggaag aagaattaat gaaccgtctt
    17881 acaggtcgtc gaatctgtga gtcatgtggt acaacgtatc atcttgtatt taatcctccg
    17941 aaggtcgaag gtatttgtga tatcgatggt ggtaaattgt atcaacgaga agatgataat
    18001 cctgaaacgg tagctaatcg tttgagtgtt aatattaaac aatctaagcc tattttagat
    18061 ttctatgatc aaaaaggtgt attgaaaaat attgatggtt caaaagatat tagcgacgtt
    18121 accaaagatg tcattgatat tttagatcat ttgtaataga tcaactttta gcggtctatt
    18181 ttaagtgagg catgatttaa tcatattaga tagatgagag tggcaattaa cgataattaa
    18241 ttcccgtaat ttttgtttaa cttataaaat taatcactga tagattcgta acaataaagt
    18301 tgtctattga cgattacctt actattgcaa aaagggggaa gttaatcaat ggctaaacaa
    18361 gatgtaattg aattagaagg tactgtatta gatactttac cgaacgcaat gtttaaagta
    18421 gaattagaaa atggtcatga gattttagct cacgtaagtg gtaaaatcag aatgaattac
    18481 attcgtattc tacctggcga caaagtaact gttgagatgt ctccgtacga tttaacacgc
    18541 ggaagaatta cttatcgtta taaataatcg tcactccata atatagggag gtataaaaat
    18601 gaaagtaaga ccatcagtaa aacctatttg cgaaaaatgt aaagtcatta aacgtaaagg
    18661 taaagtaatg gtaatttgtg aaaatccaaa acacaaacaa agacaaggtt aataaaagag
    18721 aggtgtaaat taatatggca cgtattgcag gagtagatat tccacgtgaa aaacgcgtag
    18781 ttatctcatt aacttatata tacggtatcg gtacgtcaac tgctcaaaaa attcttaaag
    18841 aagctaacgt atcagctgat actcgtgtga aagatttaac tgatgacgaa ttaggtcgca
    18901 tccgtgaagt tgtagacggt tataaagtcg aaggtgactt acgtcgtgaa actaacttaa
    18961 atatcaaacg tttaatggaa atttcatcat accgtggtat ccgtcaccgt cgtggtttac
    19021 cagttcgtgg tcaaaaaacg aaaaacaacg cgcgtactcg taaaggacca gttaaaacgg
    19081 tagctaacaa gaaaaaataa taggtaaagg aggcaaattt taaatggcac gtaaacaagt
    19141 atctcgtaaa cgtagagtga aaaagaatat tgaaaatggt gtagcacaca tccgttcaac
    19201 attcaacaac actattgtaa ctatcactga tgagttcggt aatgctttat catggtcatc
    19261 agctggtgca ttaggattca aaggatctaa aaaatcaaca ccatttgcag cacaaatggc
    19321 ttctgaaact gcatctaaat cagctatgga gcatggttta aaaacagttg aagtaacagt
    19381 taaaggacct ggtccaggtc gtgaatcagc tattcgtgca ttacaatctg caggtttaga
    19441 agtaactgcg atcagagacg ttactccagt acctcataac ggttgtcgtc caccaaaacg
    19501 tcgtcgtgta taatttatga tggtattgtt acaggtcact gagcaaacat tttaaattaa
    19561 gtcgacgtat ataaggagga tatttaaatg atagaaatcg aaaaacctag aattgagaca
    19621 attgaaatta gtgaagatgc taaattcggt aagttcgttg ttgaaccact agaacgtggc
    19681 tacggtacta cactaggaaa ctccttacgt cgtatcctac tatcttcatt accaggtgca
    19741 gccgttaagt acattgaaat tgagggagtt ttacatgaat tctcagcagt agacaatgta
    19801 gttgaagatg tttctacaat tattatgaac attaaacaat tagcattgaa aatttactct
    19861 gaagaagata aaactttaga aattgatgta cgtgatgaag gcgaagtaac agcaagcgac
    19921 attacacatg acagtgatgt tgaaatttta aacccagagc ttaaaattgc aacagtatct
    19981 aaaggtggtc acttaaaaat tcgtctagtt gctaacaagg gtagaggtta cgcattagca
    20041 gaacaaaata atactagtga tttaccaatt ggtgtaatcc ctgttgattc attgtattca
    20101 cctgttgaac gtgtgaacta tactgttgaa aatacacgtg taggtcaaag cagtgatttt
    20161 gataaattaa cattggatgt ttggactaat ggttcaatca caccacaaga atcagtttca
    20221 ttagcagcaa aaataatgac tgaacacttg aatatcttcg ttggtcttac tgatgaagcg
    20281 caaaacgctg aaatcatgat tgaaaaagaa gaagatcaaa aagaaaaagt attagaaatg
    20341 tctattgaag aattagactt atctgtacgt tcatataact gcttaaaacg cgcaggaatc
    20401 aattctgttc aagagttagc tgacaaatct gaagctgaca tgatgaaagt gcgtaattta
    20461 ggtcgtaaat ctttagaaga agttaaatac aaattagaag atttaggatt aggattaaga
    20521 aaagaagatt gataaaggag gttaactcat gggttacaga aaattaggtc gtacttctga
    20581 tcaacgtaaa gctatgttac gtgacttagc tacatcactt attattagtg aacgtattga
    20641 aactacagaa gctcgtgcaa aagaagttcg cagtgttgtt gagaaattaa tcactttagg
    20701 taaaaaagga gatttagctt ctcgtcgtaa tgcagctaaa actttacgta atgttgaaat
    20761 cttaaacgaa gatgaaacta cacaaactgc acttcaaaaa ttatttggtg aaatcgcaga
    20821 gcgttacaca gaacgtcaag gtggttacac tcgtatcctt aaacaaggcc ctcgtcgcgg
    20881 tgacggtgct gaatcagtaa ttatcgaatt agtataagaa tttatataaa tacactaact
    20941 acttttatat aaagcaaatg agtgcaacga taatgtttgc cacatacaaa tattgtctag
    21001 ctcagagtac cccatcaact aaataaagat ttaaagcgtg aactcaacaa acttatgatg
    21061 gggtgcgcgc ttttttattg aataagatat cggacttatg aacagaaatg tttttaagtc
    21121 cgatttcttt atatctactt ttttgagtgc gaatctaaaa atatgatcaa gctagtttga
    21181 tcaatttcac tagaactgaa tgaagtgaaa gtattatcgt tgaatcaaac gcggccaacc
    21241 ctatattagt aaatcattta ttttattaac atttatacat aattgtttga gaggaataaa
    21301 tgtccttatc attagttgtt aaacaactct ttaaagacta gaaatgagtc agaaaatact
    21361 agtaatatat agcaaaccca gtcaatgtca tatacagtga tttataaata ttgtaaaata
    21421 tgatagatac acgaatcgta aaggagagag acaagtggag gataagaatt cagttattgt
    21481 atttaaaaat gtttcatttc aatatcaaag tgatgcatcc ttcacattga aagatgtttc
    21541 ttttaatata cctaaagggc agtggacatc tattgtcggt cataacggtt ctgggaaatc
    21601 tacaattgcc aagttaatga ttggcataga gaacgttaaa tctggagaaa ttttttataa
    21661 taatcaaact ataacagatg ataattttga aaagttaaga aaagacatag gaattgtatt
    21721 tcagaatccg gataatcaat ttgttggttc aattgtaaaa tacgatgttg catttggact
    21781 cgaaaatcat gcggttccac atgacgaaat gcacagaaga gtcagcgaag cacttaaaca
    21841 agttgatatg ttagaacgtg cagattatga acctaatgca ttatcggggg gacagaagca
    21901 gcgtgtggct atagcaagtg tattagcact taacccctct gtcattatat tagatgaggc
    21961 gacttctatg ttagatcctg atgcacgtca aaatttattg gatttagtga gaaaagttaa
    22021 atcagaacat aatattacaa tcatttctat tacgcatgat ttatctgagg cgatggaagc
    22081 agatcatgtt atcgttatga ataaagggac tgtctataaa gaaggaacag cgactgaaat
    22141 tttcgaccat gcagaagagt taacaacaat aggtctagat ttgccattcc caatcaaaat
    22201 aaatcaaatg ctgggacacc aaacatcatt cttaacttat gaagggctgg tggatcaact
    22261 atgattatac gctttgacaa tgtaagttat acctatcaaa aagggacacc ataccagcat
    22321 caagctattc atgatgttaa tacagaattt gaacaaggta aatattatgc catcgttgga
    22381 caaacgggta gtggtaaatc gacattgata caaaatatta atgcactgtt aaagccaact
    22441 actgggacag ttacagttga tgatattact attacacata agaccaaaga taaatatatt
    22501 agacctgtaa gaaaaagaat tggaatggta tttcaatttc ccgaatctca attatttgag
    22561 gacacagtag agcgtgaaat gatatttgga cctaaaaact ttaaaatgaa tgtagatgaa
    22621 gccaaaaact atgcccatcg tctgttgatg gatttaggct tttcaagaga tgtaatgtca
    22681 caatcaccat ttcaaatgtc aggtggacaa atgcgtaaaa tagcgattgt atcgatattg
    22741 gcaatgaatc ctgatattat cgtggttgat gaacctacag caggacttga tccacaaagt
    22801 aaacgacaag taatgcgatt actaaagtca ctgcaaacag atgaaaataa gacaattatc
    22861 ctaatttcac atgatatgaa tgaagtcgcg cgttatgcag atgaagtcat cgttatgaaa
    22921 gaaggtagta tcgtgtcgca aacatcacca aaagagctct tcaaagataa agaaaaattg
    22981 gcggattggc atattggttt gccagaaatt gttcagttac aatatgactt tgaacaaaaa
    23041 tatctaacaa aattaaaaga tattgcctta actgaagaag catttgtaag cttgtataag
    23101 gagtggcaac atgaaaaata aattgattat agggcgatat ttaccaatta attcttttgt
    23161 tcatcatctt gatccaagag caaagttaat gtttgttttt ctttttatta tattaatatt
    23221 tttctgtcat tcaccattaa catatctttg ggtgtttgca cttatcttat tctttatgag
    23281 attagcaaaa attcaattat ggttcctaat caaaggttta acaccaatat ttttcttttt
    23341 aatcttcacc ttaatgatgc atatattttt aactaaaggt ggatatgtat tagttgaatg
    23401 gcatggattc acgattgaaa ctaatggtat tttagaaggg ctatatatat cacttcgttt
    23461 aattgggatt gtgatgattg caacaattat gacactatct acaagtccaa ttgatttaac
    23521 agatgcgttt gaaagattac ttgcgccact aaagatgttt aaattaccag ttcatcaatt
    23581 aagtatgata atgtccattg cgttacgatt catcccgacg ttaatggatg agttagataa
    23641 aatcattttg gcgcaaaagt cgcgtggttc tgaaataagt tcagggaaca ttgcaacacg
    23701 tattaaatca tttattccat tactagtgcc actattcatc tcagctttcc aacgcgccga
    23761 agaattagcg gtcgcaatgg aagttagggg ttatgatgcc aatgttaaga gaacgagcta
    23821 cagacagctt aaatggcaat tgagagatac gttatcttta atcatgatta tcccaattgc
    23881 aattatttta ttcgtattaa aatattcagg agtgtaaatt catgcgtata ttagtagaaa
    23941 ttgcgtatca aggaaataat tttctaggct ttcaaattca acagaatgga cgtacggtac
    24001 agcaacaatt tgaaaagcta ttacaacgca tgcataaaag acatgtgaga atccatcctt
    24061 caagtaggac tgatagaggt gtacatgcga tacagcaata ctttcatttc gacacagagc
    24121 tgaatatacc tatgtcacaa tggcaatatg caatgaaccg aacgttacct gatgatattt
    24181 atgtcaataa tgtagttaca gtcgatgacg attttcattg tcgttatgat tgtgtaggta
    24241 aacgttatcg ctacaaagta tatcaagcac aacaccgcga tccatttcaa agtggtttga
    24301 aaacatttat tcctgaaacg ttggatttag ataaaatgaa cagagcggca caacaattca
    24361 taggtacaca tgattttacg ggattttgtt cgcaaaaaac tgaagtagaa agcaaggtta
    24421 gaacattata ccaaagtgaa attgtaaaga cagatgatgg ctttgattat attgtcactg
    24481 gatctggatt tttatataac atggtgcgtg tcttagttgc atttttaata gaagtcggaa
    24541 aagggcgaca tgaaatttct gatgtgccga agctacttga atcaaagaat cgtaagaatg
    24601 ttcctttcac tgcaccggct gaaggactat atttagagaa gatttattta gatgaaaatg
    24661 agttattaaa agactttggt aacgatatca aaatacatcg taaaaaatca ttacaaaatg
    24721 actgattgcc attgacaaaa agcttgtgaa attataagat tattaacggt attgttttat
    24781 atccacccca cgataagccc cggaaactta ttgtgttaca agatatataa gcagaaacga
    24841 acaacagtta acaaaataaa tgaaattaaa cgttttaaaa atgaaacaaa tgaaatcatc
    24901 tattaggtta tgaaactgtt tatagcttga atagaagcat ttatttttta ggaggacaat
    24961 tattatgcgt caaacattta tggcaaatga atcaaacatt gagcgcaaat ggtatgttat
    25021 cgatgctgaa ggccaaacat taggtcgttt atcatcagaa gtagcatcta tcttacgcgg
    25081 taaaaataaa gtaacttaca caccacacgt tgatactggt gattatgtaa tcgttattaa
    25141 tgcatcaaaa atcgaattta ctggtaacaa agaaactgac aaagtttact accgtcactc
    25201 aaatcaccca ggtggtatca aatcaatcac tgctggtgaa ttaagaagaa ctaacccaga
    25261 acgtttaatt gagaattcaa ttaaaggtat gttaccaagc actcgtttag gcgaaaaaca
    25321 aggtaaaaaa ttatttgtat atggtggcgc tgaacatcca cacgctgcac aacaaccaga
    25381 aaactacgaa ttacgtggtt aattagaagg aggaaatgac tttggcacaa gttgaatata
    25441 gaggcacagg ccgtcgtaaa aactcagtag cacgtgtacg tttagtacca ggtgaaggta
    25501 acatcacagt taataaccgt gacgtacgcg aatacttacc attcgaatca ttaattttag
    25561 acttaaacca accatttgat gtaactgaaa ctaaaggtaa ctatgatgtt ttagttaacg
    25621 ttcatggtgg tggtttcact ggacaagctc aagctatccg tcacggaatc gctcgtgcat
    25681 tattagaagc agatcctgaa tacagaggtt ctttaaaacg cgctggatta cttactcgtg
    25741 acccacgtat gaaagaacgt aaaaaaccag gtcttaaagc agctcgtcgt tcacctcaat
    25801 tctcaaaacg ttaattgtcg gacgatatat acaaaacacc tcgaagttat gtcgaggtgt
    25861 ttttttgcgt ttgtgtgggg aatatgataa gtatgtagta taaatgaacg ttcctttatt
    25921 taatgtctca ggaatagaat aaattcaact atgaattatt gatttacacg tatattttgt
    25981 gcatgcttat acaatcaaaa gtgtacatta ttaaattgtc atttagcttg cggagggtgg
    26041 acgataatgt tctaatcgta gtggttcaag ggaaagggct atcggatgaa aatcggaaga
    26101 taactcaaga gccatgattt aagagtgtta tcaaaataag gagtgactta attgaaagat
    26161 tctatatttt ggaagaaagc ttttattcct gtttatttta ttgttgcgat gctggtgtta
    26221 atacttttta ggttttatat taaaactgat aacttttctg tatatttaat gattatcttc
    26281 ttaatgtgtt tcggaactgc atctatcatt tataa
//
LOCUS       DAPIDM010000022        25768 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000022 DAPIDM010000000
VERSION     DAPIDM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.022"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(504..959)
                     /locus_tag="RJE42_000852"
     CDS             complement(504..959)
                     /locus_tag="RJE42_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDV5876296.1"
                     /translation="MNNEILQRMVENLSEEKFGRTFRHRAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(952..3102)
                     /locus_tag="RJE42_000853"
     CDS             complement(952..3102)
                     /locus_tag="RJE42_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV5876297.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTAVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(3537..4307)
                     /gene="sigB"
                     /locus_tag="RJE42_000854"
     CDS             complement(3537..4307)
                     /gene="sigB"
                     /locus_tag="RJE42_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDV5876298.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            complement(4282..4761)
                     /gene="rsbW"
                     /locus_tag="RJE42_000855"
     CDS             complement(4282..4761)
                     /gene="rsbW"
                     /locus_tag="RJE42_000855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDV5876299.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(4763..5089)
                     /locus_tag="RJE42_000856"
     CDS             complement(4763..5089)
                     /locus_tag="RJE42_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDV5876300.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(5208..6209)
                     /locus_tag="RJE42_000857"
     CDS             complement(5208..6209)
                     /locus_tag="RJE42_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDV5876301.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(6559..6921)
                     /locus_tag="RJE42_000858"
     CDS             complement(6559..6921)
                     /locus_tag="RJE42_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV5876302.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(6918..7088)
                     /gene="mazE"
                     /locus_tag="RJE42_000859"
     CDS             complement(6918..7088)
                     /gene="mazE"
                     /locus_tag="RJE42_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV5876303.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(7173..8321)
                     /gene="alr"
                     /locus_tag="RJE42_000860"
     CDS             complement(7173..8321)
                     /gene="alr"
                     /locus_tag="RJE42_000860"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV5876304.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYHRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVIGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(8387..8746)
                     /locus_tag="RJE42_000861"
     CDS             complement(8387..8746)
                     /locus_tag="RJE42_000861"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV5876305.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(8750..9241)
                     /locus_tag="RJE42_000862"
     CDS             complement(8750..9241)
                     /locus_tag="RJE42_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDV5876306.1"
                     /translation="MSDYNFMSPHAKKVMRLSAVLFWIPIALVILIAFNILNWLFWEI
                     LDNYISIVSSVIVILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(9234..10811)
                     /locus_tag="RJE42_000863"
     CDS             complement(9234..10811)
                     /locus_tag="RJE42_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDV5876307.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFVIFQLKDFDYTNPV
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVINQMIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSST
                     EQNDNCNHDIEGNVEDIAKGRLDEVQNTNSILNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIAAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVTILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVITMAGIGCYFWNQLLLLFTIITVVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     KDVQILKSWYLRGENDE"
     gene            complement(10804..11283)
                     /locus_tag="RJE42_000864"
     CDS             complement(10804..11283)
                     /locus_tag="RJE42_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876308.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPQYLIYILFVLCTLTVLKLIIKPLWQYHCRFYQVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARAMSNLRGVESDV"
     gene            complement(11492..12052)
                     /locus_tag="RJE42_000865"
     CDS             complement(11492..12052)
                     /locus_tag="RJE42_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV5876309.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNVVSSGGSNESNGNTELIA
                     RVKHHVKFGNSNVTIDATTSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(12072..14099)
                     /gene="kdpB"
                     /locus_tag="RJE42_000866"
     CDS             complement(12072..14099)
                     /gene="kdpB"
                     /locus_tag="RJE42_000866"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV5876310.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSQGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNELSVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFAELRKMGIETVMCTGDNALTAATIAKETGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISAMIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFIGIKLIDMIVQFFV"
     gene            complement(14118..15794)
                     /gene="kdpA"
                     /locus_tag="RJE42_000867"
     CDS             complement(14118..15794)
                     /gene="kdpA"
                     /locus_tag="RJE42_000867"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDV5876311.1"
                     /translation="MEIILFLTMMVMIAYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLVFNGFMGVITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALLLFVAMFFIFIAILTLTMWSEYRGNPILANLGIHGANMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGANESITNPSFH
                     GISQIMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(15818..15928)
                     /gene="kdpF"
                     /locus_tag="RJE42_000868"
     CDS             complement(15818..15928)
                     /gene="kdpF"
                     /locus_tag="RJE42_000868"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV5876312.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            16066..18723
                     /locus_tag="RJE42_000869"
     CDS             16066..18723
                     /locus_tag="RJE42_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDV5876313.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLLKIATNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIERMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     ISRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KRRDFFNKPLIDHLMSFEHSYNIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSYIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYAITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISLKEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDI"
     gene            18723..19418
                     /locus_tag="RJE42_000870"
     CDS             18723..19418
                     /locus_tag="RJE42_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5876314.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNFVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(19771..21291)
                     /locus_tag="RJE42_000871"
     CDS             complement(19771..21291)
                     /locus_tag="RJE42_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV5876315.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     DSESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(21807..23162)
                     /locus_tag="RJE42_000872"
     CDS             complement(21807..23162)
                     /locus_tag="RJE42_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV5876316.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQKGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNEHYDL
                     PILGKHNMKNATIAIAVGHELGLTYNIIYQNLKNVSLTGMRMEQHTLENDITVINDAY
                     NASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFG
                     NEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALI
                     S"
     gene            complement(23177..24247)
                     /locus_tag="RJE42_000873"
     CDS             complement(23177..24247)
                     /locus_tag="RJE42_000873"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV5876317.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITTEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            24565..25767
                     /locus_tag="RJE42_000874"
     CDS             24565..25767
                     /locus_tag="RJE42_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDV5876318.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
BASE COUNT         8302 a         4776 c         3558 g         9132 t
ORIGIN      
        1 aaaacattaa gaacttattg tcaacatatt ttataaatta aatttagaat tgactgtctt
       61 tcaaatcaaa gtttatttaa tttgttcttt tttaaggaac gtgtttaatc atactaacta
      121 taagctgtac agtcaataga taaattcaat caaaatgtca ttaaaaatgt ataattatct
      181 tttgtgccgc tttaaagctt taattgggta aagtttatat ataattcatt actaaaatac
      241 gccaagattt ttaatctaat ctatttaatt tcatcctatc aattcacaat ttatgccatt
      301 acctatatta aaagtagaaa agccgcgatt gcgaagtatt aaattacgac aatctcggct
      361 ttaaacattt tataaacaca atttcttctt attcaattac tatgtttacc aatcaaaata
      421 actcactgat aaattctata taaattccat acaactcata aataatctta catatacatg
      481 aattagctct atttgtattg tacttatttc aattgtcttt tcattcttaa ttttccattg
      541 caatgtccgc aacgcatacg attcgtatca actttacgga tacgtatata ttttgcatga
      601 catttagtgc aataatattc ataatttgcc cgttgttgat agctttcaat gctgttacaa
      661 aatctaggcg ctccaacctg ttggctcaat cgtttaaaat cttgatcttt atgttgataa
      721 cctttaccag caatatgcaa gtgataatga cacagttcat gcagtataat ttttacaaca
      781 gcatcttctc cataatgctc atattgtttt ggattaattt caatatcatg agactttaaa
      841 agataacgtc cgcctgttgt acgtaacctt ttattaaaat atgcacgatg tcgaaacgta
      901 cgtccaaatt tttcttccga aaggttctca accattcgtt gaagtatttc gttattcatg
      961 tggatcaatc attgttaatg atactttgtc tttatttttg tcaatactgt aaatccaaac
     1021 gtcaacgata tcaccgacac tgacaatatc cattggattt tttacgaatt tcttagaaag
     1081 tttcgaaaca tggacaagtc catcttgttt aacacctata tctacaaatg ctccaaaatc
     1141 tactacattt ctaactgtgc cactcaattt cattccttct tttaaatctt caattgatag
     1201 cacatcggat ttaaggattg gtgtttcaaa ctcgtccctt ggatctcgat taggtgcttt
     1261 caaggattta ataatatcct ctaatgtagg tacaccgact tgtaattcaa tcgccagttc
     1321 atccatatcc aatgaattta atttagcttt taatgcatca ctacctaaat cattgccacc
     1381 aaatccgagt ttatctaata actgatacgt tactttatag ctttcgggat gaatcgatgt
     1441 attatccaat ggctctgacc cgtctactat tcttaaaaat cctatactct gctcaaaagt
     1501 tttagcaccc aaacgcttaa tcttgcttaa ctctttatta tgctttattg cgccattttc
     1561 ttctctatat gcaattatat ttttagcaat ttgcgaactt agaccagaca catattgtaa
     1621 taatgatgat gatgctgtat tgacatcaac acctacttgg ttaactgctg tttcaacaac
     1681 gaaagttaat gcattttcta gtgctttttg attaacatca tgctggtatt gtccaacacc
     1741 aatagattta ggatcgattt taactaattc acttaatgga tcttgaacgc gtcgtccaat
     1801 tgatacggca cttctttctt ccacttgaaa atcaggaaat tcatctctag caatttctga
     1861 tgctgagtat actgaagcgc ccgcttcatt gacaatgatg aattgtactg gcaactgatg
     1921 ctttttaatt aaatctgcaa caaattgttc tgtttcacga cttgctgtac cattgccaat
     1981 tgcaattaat tgcacatcat acgctttaac catttgtaca aaatccttct ctgcagcttc
     2041 ttttttagaa actggtggat gcggataaat cacacctttt gctataaaag taccgaatgg
     2101 gttaatgact gctaatttac aacctgttct aaatgcagga tctacgccta atatttgttt
     2161 acctttcatt ggaggttgca gtaatagatt tcttaagttt tcactaaaaa catctattgc
     2221 atgattttca gctttttcag ttaaatcagc atggatttca cgctctatcg aagggacaat
     2281 taagcgtttc aagctatctt taatcgcctc taaaatataa ctgcgattta cattattatg
     2341 attgatttct tgacgtgcaa tgaagtcctc tactgctgtc gtatcgaatt caaactttac
     2401 agataatact ttctcttttt cgccacgatt cacagctaaa accctatgat tagcaatgcg
     2461 tttaattggc tcactatatg catagtacat ttcaaaaata cctttttcat cttcagcatt
     2521 tttcttttta gatgtagtta acacaccttg atgatacata tcttttaaaa tttttgttct
     2581 atatttagga ttatctgaaa tttgttccgc aataatatct tgcgcacctt tgatagcatc
     2641 ttcaaccgat tgcacttctt catttataaa ttgttgtgct ttttcttcaa ttgagacttc
     2701 atgtttacgt gccttcatcc atatcgctaa tggttctaat cctttacgtt tcgcctcagt
     2761 tgcccttgtc tttttctttt gtttaaaagg tctatatagg tcttcaacac gttgtaattt
     2821 gttctgtttt aaaatatctt tctttaattc ctcagtaagt aatccttgct gttctatatt
     2881 tttgataact tcttctttac gtttttgtaa attgaccata tattggtatt cgtcatcaat
     2941 ttgctttatt tgaacttcat ctagtccacc agtttgctct tttcgatacc tcgcaataaa
     3001 tggtactgta tttttttctt caagcagtgt taatactgct tcaatttgtt ttttactaaa
     3061 ttgatatttc tctatgattg aattaatcaa ttgattgtcc atttacttaa cccacacttt
     3121 ttcttaaatt tactcattta ttttaacata ttctaaaata cttctattaa ggtatgatac
     3181 ttaatgcaat tttaactttc aaaacattaa aaacgaatta ttaggtcata ctatatttta
     3241 aaacgcacta agattattga aaatgacaca ccatcatttc ctaaatttca taatctaagc
     3301 ctactttttt tagtctaata gcaatccacc tatttaagcc gacaattaga tatcttcttc
     3361 acttaaatta ttaatactat atcgcgacat ttatgtggat acacattaaa ctacgttaat
     3421 aatgcattaa ttcgaaaatc ttaattaaat taaaaatacc cctcgatttc aattgactaa
     3481 tcaagggata attgtatgaa tttttcatta taaaacgcat cattaataag caaattctat
     3541 ttatgtgctg cttcttgtaa tttcttaatt gccgttctct gaagtcgtga tacatgcatt
     3601 tgacttaaac cgatacgctc acctgtctct ttttgactca aaccttcaat aaacgtacat
     3661 tgtatgattt ctcgttcgcg atcagataat ataggtaata ttttttctaa aatcatacgt
     3721 ttttcagtta agtcataatg gtcatcttgt tgccccataa tatctaatag cgtaacagtt
     3781 gaaccatctt tatcagcttc aatggaatga tcaacactta acgcattata actttgtccc
     3841 atttccattg cttctaacac ttcttcttct gaaacttcta atcgatcagc tatttcactg
     3901 atagaaggtg aacgctctaa ttcagcggtt agttcatcgc tcactttttt gattcttggt
     3961 ccaatttctt taatacgtct cggaacatgt acactccaag ttttatctcg tagatatctt
     4021 ttgatttcac cgattacagt aggtactaaa aaggcttcaa acttccgttc aaaggacata
     4081 tcgaatctat ttatggcacc tattaaacca accataccaa cttgaactaa atcttcgtga
     4141 tgtgattgtc ctttagaata tttatatgcc aatgactcaa ttagtttttg gtaatgttta
     4201 actaacttat cttgtgcatc tgtattctta ttttcttggt gttctttaat ccattggtta
     4261 atttgctcag gtgaaacttc attagctgat ttcgactctt tcgccattat ttcgcacctg
     4321 ctcttttttt atatacttag tcatactgat tgtcacacca gattctttat atactgtcac
     4381 ttcatccatt aaagattcga ttaaaaataa acctaggcca ccttcacgta aaaagtctat
     4441 attttcgtct ttatcgtaag gacctatttt tgatttagtt gtttcataat caaaactgtc
     4501 acctttatca gaaataacaa ttttaatttt atcttctaaa atttcaaaat atatgttaat
     4561 aatgcccaca ttgttttttt ctttgtatgc atgtttaact gcatttgtca cagcttcact
     4621 aactgcaatc ttggcatctt caatatcatc atatgtagca ccagctctcg aaaaaacgcc
     4681 agaaagtgtt aaacgaatta aacttacata ctctgccgat gctggcacgc gcatttcgat
     4741 aaaatcttct ttagattgca tgttattcga cctccgttcc ttcattaaca tgcattaaat
     4801 ccttaagacc agtaatttca aatagtctac cgatacgatc tgacacacct aaaatgtata
     4861 gttctttatc attttggttt aatgctttta atgtacctac gaataaacct aaacctgtcg
     4921 aatccatata actcacattt tctaaattaa cataaatatc acgagttcca tcttgtctca
     4981 taggtgttaa aacctcttct aattcaggca cagtataaac atctaattct ccaccgactt
     5041 taacttcgta aaatttatct tgagtggttg tttctatatt aagattcatt tcattacact
     5101 cctacttatt aaaaatattt atcttcattt taagcgctgt atccaccata tttatatttc
     5161 aaattattat atacccatcg aaataatttc taatcttctt ttttaaatta atttactctt
     5221 tttataatca aaatagtcat atcatctttt ttatttgggt tttgaagctt taaaattgct
     5281 tcatagataa tttgaacaat atcttgtggg tgcatatgtt tatgtttttt aatatattct
     5341 aaaagttttt gtttatctat aaaggtacct tcactatttc tagcttcagt cacaccatcc
     5401 gttaaaatga taattaaatc atcaaggtat attggaattt cttgttgttg atatcgtgtt
     5461 tgtgaactga ttcctaacac tctacctcta actgaaattt cttcaaattc ttctttttca
     5521 gcgcgataaa tatatccagg ctcatgacca gctgaactac aatacaataa atggttcatt
     5581 tcttcatata aaccataaaa cattgtgacg aacatatttt gattaatatt cttttcaaca
     5641 acacgattta aacgttttaa gccatcactc ggtaattgtg agtgtccata agaatccatg
     5701 ccaaacttta tcatactcat tgctaaagca gctggtatac cttttccaat gacatctgca
     5761 acagcaaagc tcattgtgcc atcgttatgg tcaattaaat taaaataatc tccacttact
     5821 ttttgtgccg ccactgaaat aacgccaatt tgaatactat caaattgtgg aatatctgtt
     5881 ttaagcattg tttgttgtaa gctagaagct aagtctatct ctttatcgtg aacttgaagt
     5941 ttatctacca atctttgata atctcgataa ctataaccaa agcctttaac gatttcttgt
     6001 aagacatcta atgtcttgaa cacatcttca cgcgttaagt ttaacgtcaa tatatagttt
     6061 ttgtgaatat cgacaatgtc ttcaggcaag acgtctttac gaatcacttc gtcagtgtat
     6121 ttctcacact tttttatcaa ttctacttta tcttggcacg ttaaactttc atcaattaaa
     6181 cccttataat gttgcttaaa ttcttccacg attagttgcc tcctcacttc ccttacaaag
     6241 caaatgctta agcaaggata attatgattc acttactaac atttatttaa ttgcacttat
     6301 caattttgtc gtttgcaatt ttgctgtaga tgatactttt aagctaaaaa aaactttagg
     6361 atggaatacc taaagcctaa aaaacttaag acatcctttt ataataaaaa atatgaatat
     6421 cataactggt atttgctaaa atctttgtat ataagaaatt ttttcgtaca gaatttagaa
     6481 atagttataa ttattataaa gttattgtct tttatatcaa tattttattt atctctgaaa
     6541 aatacatata atagacgcct aatttttctg atgagctact gcatttagcc ctaaactaat
     6601 cattagtgca ttatctactt ctttcatttt atcatcggat aagtacgtca gtttttcttt
     6661 caatcgtttt ttatcaagtg tacgaatttg ctctaataat ataactgagt ctttatccaa
     6721 tttatacttt ttcttttcaa tctctacatg tgtcggtatt ttcgctttgt taatcctacc
     6781 agttattgcc gcaacaataa ctgtaggact atatttatta ccagtatcat tttgaattat
     6841 gactacaggt ctgactcccc cttgttcaga tccctgtact ggtgataaat ctgctaaata
     6901 aacatctcct cgtctaatca ttcattcgtt gaattagaag ataaatatgt ttcgttgcaa
     6961 tcgcatgctt cacactctat cggaaaagct tcgttcgcta gggagagatt taaatcagcc
     7021 atttgtgaat atccttcttt taaagattgt tctaagctat gacttctatt ttgactaaaa
     7081 gataacatat gagaacctcc aattgattcc aaaagttatt aaatgacctt taattactct
     7141 aatgataaca aattttatat gcactgacca tattatttta acaattcatt tgttacaaaa
     7201 cgttgatcac catcatgata gattcgcggc aaacgtctcg acaagttaca aagcacttca
     7261 taattaatag tatgttgctt ttcagctgcc acctctaccg actgtggact ttctctatga
     7321 ttatctatta aaatcaccga atctccagct ttaacttgat ctggcacttt tacaattgtc
     7381 tgatccatac atacgcgacc aataacttcg cattgatgac caattacatt tacaaagcta
     7441 ccttgcataa tgcgtaaata gccatctgca tatccaatag gtaacaatgc tattgttgtt
     7501 ggatcagtag ctgtataagt tgcaccataa cttacagact cacccgcttg tagcgtcttt
     7561 gtttgaacta cattagcaat taattgcaca cttggtttaa ggtgtacttt aactttttgc
     7621 tgtacatact ctgatggata atatccataa agggaaattc ctggtcttat tgcattacag
     7681 aattggcaat ccattaatag agaacctgct gagttctgac aatgtatata ttcaggttta
     7741 attgcttcat tgaccatatc tttaaaacgg tgatattgtt cagttgtcat atctcctggt
     7801 tcgtcagcac aggcaaagtg tgtaaacacg ccttcaaata caagttgctc atattgttga
     7861 atgatttcaa tcacttcttg atacgtttta gtatctttaa tacctaaacg tcccattcct
     7921 gtatctaatt taatatgcaa ccataacttt ttctcttgct caccagaaat gtttttaatt
     7981 gcttctttca accactgttt agacggaacc gttaaggcaa ctcggtgttg tatcgcttta
     8041 tcaatatctt tagctggtaa cacacctaag actaaaattt tagcagtaat cccatgcatt
     8101 ctaagttcta tcgcttcatc taacgttgct acagcaaaaa atgtggcacc attttccatt
     8161 aaatgacgtg ctactttaac actacctagt ccataggcat tggctttaac gacagccatc
     8221 actgttttat ttggatgcaa tgtactgaat actttgaaat ttgatgcaac agcgtttaaa
     8281 tctacattca tatacgcaga tctataatat ttatctgaca tattacttcc tcctgtaatt
     8341 cccacacgtt ttaaaactag attttaatta tcattgtata acaaatttaa aatgctgact
     8401 tttctaaaac aacttggctc atcgcataat gctcagtgtg tgagatacta acatgtacga
     8461 tgaacccttc gtaatcaatc tttggtttgc caagttcgtc gttgtaacaa tctatgtcat
     8521 taaatgcgac atgttttcct aagcctgtgc ctaatgcttt actgaacgct tcttttgtag
     8581 caaacctgcc agctaaaaat tcaattttac gttgctcatg tgtgaaattg ttgaatttgt
     8641 gctgttcatt tttagttaaa atccgctcaa ccaatttggg ttgcttacta tataacactt
     8701 ttattcgatc gatttcgatt aaatctacac caattccatg tatcattgat tattcctcat
     8761 ttttattgtt ttctaaatgt ctctcgttat ttaaccagcg tttcaatcta cgtgcttcac
     8821 ttctatctat taattcaatt tcagcttgac cacccgctgt ataaagtgtc aaagttgcta
     8881 atcgatagcg tctcattata ggaccaacat cgatatcaat attttgaata cgaaaatatg
     8941 gtattacttt ttcatccaaa aataaaatgc cgtttcgtac acgcaaatgg tgtttttcaa
     9001 acgcatatct gcagtgctta tatcgataga ctggtgcaat gacaagcgtg aaaacagcaa
     9061 caagtaatat tacaatcaca ctactaacaa tggatatata gttatctaat atctcccaaa
     9121 atagccagtt caaaatatta aatgctatta aaattacaag tgctattggt atccaaaaca
     9181 gcacagcact taacctcatc acttttttag catgtggtga cataaaatta taatcactca
     9241 tcattttcac ctctcaaata ccacgacttc aatatttgca catctttact tcttgcgtat
     9301 tttaagttaa tcgattgggc tcccacacct ttagcaacaa taaaactaaa attatttaaa
     9361 ttgttgcgtt taagtaatgt atgttgccaa gtgtcaaaac ctataatgtg atgcgcttta
     9421 aaataataaa tatttcgttt caatagctcg aaattctgga tagtaatttg ttcttctgtc
     9481 attttaaaac ccgcatgttt aacataaaga tatcctttga tcacaaataa accaataatg
     9541 actactgtta taatcgtaaa taacaataat aattgattcc aaaaataaca gccaattcct
     9601 gccatagtta tgacaataat actaggtatt aaaaagtgtc tgtggaaacc tgacaaaggc
     9661 ataccttcat taacttgttg ataagataaa tctggtacta aattctggat aatttgatat
     9721 gctttgtctc gtttaataaa cggcaatatc gtcacactac ctgaatcatt gtcatcatct
     9781 tcgatttttt tattttcaaa atcacttgtg atgataaaat gtattgctgt gtagccaaat
     9841 aatcttcgta tatatgactg acgttcaaca acagcttgca atcgtttcgt tggtacagtc
     9901 actttcttct tgttaaagag accatattca attttaagtt gttcattttc aagcatcacc
     9961 gaaaagccat aaaatcttat catcgttata atcgttccaa taatatatgc cgctattaat
    10021 actagtaaaa tgatgattaa tactgaaata cttacaattt gaacccattg actaatttca
    10081 tggtttaact tcgaccatgg aatcaactct cttacagcgc cgtaaatcgg tactaaagct
    10141 gctaacgtta caccaatggc gccactggtc attgccataa atagtgattc tttaaaattc
    10201 atctgatata taggaatgcg tttatttttc tgattaagta tactattagt gttctgcact
    10261 tcatctaagc gaccttttgc gatgtcctcc acattacctt caatgtcatg attacagttg
    10321 tcattctgct cagtactaga cttttgcgcc acttctgtct tcaactctgt ttgcaattga
    10381 tcaatatagc gttcaagata ttcaccttgc tttttcgaaa taacacttaa cacaatacca
    10441 tcacttggcg ttttgatttg taaatccacg ccacctatca tttgattaat gaccccttgt
    10501 gtcatatcaa cggactgaat acgttttata tttaattctt tacgcttttt attaaaaata
    10561 cctgttgtta aaatgaaata attatcctca atccaatatc gcgtgttcat aattccgaca
    10621 atttgagaaa tgtatgatat taaaaagaat acaaatacaa tacctatcca taaatatgat
    10681 acgggattcg tataatcaaa atctttcaat tgaaagataa cgaaaataaa aaagacgact
    10741 atgttttgtt tgatagcatt gattatgcca ttaaaatatg aaatcggatg taatttttga
    10801 ggttcagaca tcactttcaa cccctctcaa attcgacata gctctcgctt cgattatttt
    10861 aacatcgtca tgagacatca tcggtaaata aatagtatga cctgcagtca taaatccaac
    10921 tttatacaaa ttaagcactt tactaattgg attagattta atcgacaagt attgtaaacg
    10981 ttcaattcga ctcgtttctt ctttatatat taaaaatgat gtacgatatt gtacactgag
    11041 ttgatcaact tgataaaagc gacagtgata ttgccataaa ggcttaatga ttaattttaa
    11101 aacagtcaaa gtacataaaa caaataaaat ataaattaag tactgaggcc attcaaatct
    11161 taaccatata aaataaaaaa tgacatacac agctacactc aatataaatt ctaagccatt
    11221 cgtaatgtag taatacaaca atgctgactt aggactctta gataacttag tataatctga
    11281 catatacccc tctccccaaa taaaaattat acggatttat aatctatttc attttatttt
    11341 tatatgttga taattatagc atatggaata tttcatgcta atttattctt cctaaaggta
    11401 catctaaaaa tttagttaag cagaaagtac ttgaatacct aaaaaagaca ccatgttata
    11461 attttatcaa catgatgtct ttcatttata ttcaatcttt catcttatca agagcaatat
    11521 ttagttcaag cacattcaca taatcatttg ttaacacacc acgctgctta cgatgttgaa
    11581 tcaagtcggc cactcttgaa gtagatatat gacgagcatc agcaatacga ggtgcttgct
    11641 tcaatgcatt ttcaaccgta atatgcggat ctaagcccga cccagaactt gttgtagcat
    11701 ctattgttac atttgaatta ccaaatttaa catgatgttt cacgcgtgct attaattcgg
    11761 tgtttccatt cgattcatta cttccacctg atgatacgac atttttatat aatttttcag
    11821 gattcatatt ataatcaact gcactcggtc tcccgtgaaa atatcgtgtc tctgtccagt
    11881 gctgtccaat caattttgat ccaactatac gattgtcata cgtaattaaa ctaccatttg
    11941 cttgttgata aaaaaatatt tgaccaatta acgtgatagc taacgggaat aaaaatccac
    12001 ataataccat agttattatt gttaaacaaa tactatttct tatcgtattc atggtacagg
    12061 ctccttcctc tttacacaaa aaattgtaca atcatatcta ttaatttaat gcctataaac
    12121 gggacgatta atccacctaa tccataaatc aacatattat ttataaaaat tctatcaatg
    12181 ctgtaaccct ttacttttac acctttcatt gcaattggaa ttaaggcaac aatgattaat
    12241 gcattgaata tcatagctga tatgattgca gatttaggtg acgataaatg cataatattt
    12301 agtgaagtca tttctggaat cgttgacatc attagtgcag gtaaaattgc aaagtatttt
    12361 gctacgtcat tagccaaact aaatgtcgtt aatgcacctc tcgtcattaa taattgtttg
    12421 cctattttta caacctctat taactttgta ggattcgaat ctaaatcaat tagattggcc
    12481 gcctctttag cactaattgt ccctgagttc atagctaatc ctatattcgc ttgtgctagc
    12541 gcaggtgcat catttgtacc atctcctgtc atcgcaacaa tatggccttt cgcttgttca
    12601 tctttgatga ctttaatttt atcttcgggt ttacactctg caacaaatct atcaacaccc
    12661 gtttcttttg caattgtagc tgctgttaaa gcattatcac ccgtacacat aactgtttca
    12721 atccccattt ttctcaattc agcaaatcgt tctacaagac catctttaat cacatctttt
    12781 aaataaatca cgccaagcat gacgttgttt tcaatgacta ttaatggtgt gccaccttta
    12841 ctcgatacat ccatacagag tgactcaata ttaagaggaa tattgccttg ttgttgtttg
    12901 acaagattta tcatactatt aggtgcacct ttgaataccg ataattcatt tgtaatgatt
    12961 ccgctcattc tagtttcagc tgtaaaaggt ttatatgtgc catcaatgtc tttaggcagc
    13021 tcatttatat acatctgctt cgctaatcga acaatacttt ttccttctgg cgtatcatcg
    13081 tagattgatg acatataagc agcgactatc aatttttcca gcatttgttg attcactggt
    13141 aaaaattcac tagcgattcg attgccataa gtgattgtac ctgtcttgtc taaaatcatt
    13201 acatcgacat ctccacatac ttctacagca cgtccacttt tcgctaatac attgaattga
    13261 gtaacacgat ccatgcctgc aataccaatc gccgataaca aaccaccgat tgtcgttggt
    13321 attaaacata ctgttaacgc aatgagcatc gcaataggta aaattaaatg caggtaagat
    13381 gctattggat ataatgttac aataacgact aaaaatataa ttgttaacgt tgttaataat
    13441 gtaaaaagtg caatttcatt tggtgtttta tttctttccg ccccttcaac taaggcaatc
    13501 attttatcta aaaaagatgt acccgcttca ctctcaacac gtatttctaa ccaatcagat
    13561 gttacaagtg taccgccaat gactccatca aaatcgccac ctgattcttt tatcacaggt
    13621 gcagactcac cagtaattgc agattcatca acggttgcta atccatttat tacaacgcca
    13681 tcagcaggga ttgtttctcc attttctacc cgaatatttt gtccggcttt taactctgtg
    13741 gcgttcacta tccgatacgc accattttct tctatcaatc gagcagttaa atttgattgt
    13801 gcttgtctta aactatcagc ttgcgctttt ccacgacctt cagcaaaggc ttctgaaaaa
    13861 ttagcaaaca atatagttat taataatatg ataaaaattg taatcaaata accttgcgat
    13921 agatagctag ttccaaatat gtcaggaaaa catattaata tcaacgttaa aatcattcca
    13981 acctcaacga caaacattat cggatttttt attaattgtt taggattcag cttataaaaa
    14041 ctcattttca aagcttctat caccattgtt tgattgaaat atttatttac atgatgcatt
    14101 ttttccatcc ctttacttta ttttaaagtt aaaaattcac caataggacc aagtaatagt
    14161 actggaataa atgtcaaacc acttagtaaa acgataaata cgattagtga tacgccaaaa
    14221 taaggtttat caatcgctat tgtatattta tcttggtggt atgatttttt attcactaaa
    14281 cttgatgcaa tcattaattg caaaataatt ggtatataac gagaaagcaa cataatgatt
    14341 cctgtagaga tattccagaa tgttgtatca tctttcagtc cttcaaaccc tgatccattg
    14401 ttcgcagcag ctgacgtcat ttcatacata atttgtgaaa taccatgaaa agacggattc
    14461 gttatacttt catttgctcc aggaatcata aaagcaagtg ctgaaaatac taaaattaaa
    14521 attggatgta tgagaaagac taagacaata catttcattt cacgggcgcc aattggcata
    14581 tttaaatatt ctggtgtttt accaaccatc aaactgcata taaacaccgt cagtaagaca
    14641 tatatcaata aattcatgag tcctacgcct tcgccaccaa atacaacatt tagcatcatt
    14701 aataccattg ggcctaatcc acctagaggc gttaagctat catgcatgtt attaacagaa
    14761 cccgttgtga atgccgtcgt aataactgta aatagtgctg acaaacctgc tccaaaccgt
    14821 acctctttac cttccatatt cgctccatga attcctaaat ttgctagtat tggattacca
    14881 cgatactcac tccacatagt taatgtaaga attgctataa aaatgaaaaa cattgcgaca
    14941 aataataaca gcgcatgacg atgtactcgt ttaccatgtc tacttaacat gcgaccaaat
    15001 aagaacaaca ttgacatagg aagtaacatc atactgccca tttctataaa attgctccaa
    15061 atatttggat tttcaaaagg tgttgcagaa tttcctgcta aaaatcctcc accattcgta
    15121 ccaagatgtt ttattgattc aagtgatgca ataggtccaa atgcaatatg ttgaatatgt
    15181 ccgcttaaag tccgaatcat taaattagca tgcaacgttt gtggtacacc ttgagtcatc
    15241 aataaaatac taattaaaca tgataatggt aaaagtactc gaacaataaa tcgaacaata
    15301 tcttgataaa aattaccaat gatattagtc aatccagtta aacgtctcaa catcgctata
    15361 caaacggcgt aacctgatgc actagatgta aacattaaat atgtcattac aatcatttgc
    15421 gttaaatatg tcacatctga ttcaccgtta tagtgttgta aattactatt tgttaaaaaa
    15481 gatattgctg tattaaacgc taaatctatc gattggttta aattatgatt tggatttaaa
    15541 aaaagccatt gctgaactat tagtaataca aatgttataa cccccataaa tccattaaat
    15601 accagaaaat gtttgacata tgttttagct gacatgtgtt ctaaatctgt gccgataatt
    15661 ttaaaacaca tattttcaaa tctagtaaat attaaatcta ctctcgacga ttgcaccaat
    15721 gctacgcgat atagatatcc actaaaaaca tatgcaatca taaccatcat tgttagaaac
    15781 aaaattattt ccatgataac cctcacttaa tatatttcta aaatttttca ctacgaatta
    15841 aggcataaaa taaatacaaa actaatgcaa taactaccaa taataaaacg atgagcattg
    15901 ccataacctc cttacaacac aacaacatcg taacaacttg tttatgagac aaatattaat
    15961 tttcaaactt agttattaag aaatcattaa gatgtgtatg cagaaataaa ttttatagca
    16021 tttaattgtg aagaatatta tgatattgct atcgaggtga aggttatgtc aaacactgaa
    16081 tcgctaaaca taggaaaaaa gcgtggatct ttaacaattt acatcggtta cagtccaggt
    16141 gttggtaaaa catttgagat gctttcaaat gccattgaac tatttcaaag taacgttgat
    16201 attaaaatag gatatattga accgcatcaa cgtgatgaaa caaatgcatt agctgaacaa
    16261 ttacttaaaa tcgcaaccaa ttttactaaa catggtagtc atcattttca atatttagat
    16321 gtcgaccgca taatcgaaga atcgccgaca atagtactta tcgatgagtt agcacatacg
    16381 aacatttcta gagatcgtca tgagaaacga tatatggata ttgaagaaat tttaaatcat
    16441 ggtatcgatg ttcatactac tttgaacatt caacatattg aaagtttaag tagtcaaatt
    16501 gaacggatga ccggtgtaca tgttaaagaa cgtgtacccg actatttcat aatgagcgcc
    16561 gatgtattag aagtcgtaga tatctcacct gaacaattaa ttaaacgatt aaaagctggc
    16621 aaggtatata aaaaggatag gctagatgta gcatttagta atttctttac gtatgcccac
    16681 ctaagcgaac tgcgtacatt gacgttaaga acagttgccg acttgatgag tgataaagaa
    16741 aaagtccgac acaatcataa aacgtcactc aaacctcata ttgctgtggc aattagtggg
    16801 agtatttata atgaagcagt aattaaagag gcattccata ttgctcaaaa agaacatgca
    16861 aaattcactg ctatttatat agatgtattc gaaataagca ggcaatataa agatagtcaa
    16921 aagcaagtgc atcaacatct catgcttgca aaatctttag gagcaaaagt aaaagtagtt
    16981 tatagccaaa ccgttgcatt aggattagac gaatggtgta aaaatcaaga tgtaaccaaa
    17041 ttaattatcg gacaacatat tagaaataag cggcgagact ttttcaataa acctttaatt
    17101 gaccatttaa tgtcctttga acatagctat aatatcgaaa tcgttccaat caaacaaata
    17161 cctgttgaat tgaaaatgaa caaatcaccc tatcgtccta aaggcaaacg gttcgccata
    17221 gatatgttaa aaatgattct gattcaaata atttgtgtaa tgatgggact gtggatttat
    17281 caacttgata agcatgagtc tagtacgatt attttaatga tttttctcat cggcatcatt
    17341 ttgttatcca tttggacgcg gtcctacatc attggctttt tagcagcaat tattaacgta
    17401 tttgttttta attatttttt tacggaacct agatatacat ttgaagtata ccgctttgac
    17461 tatcctatta catttatcgt cagcatttta acaagtattt taacgagtgc tttattaaaa
    17521 caaattaaat tccaatacgc cattactaaa aagcaacttt atcgtacaga tttattattt
    17581 caatttaatg attcgattaa acaaacttat acagttgaaa acttactaat aaatgctgga
    17641 taccaaatta atcaattatt gcaacaatca attactatat acgttatcaa tcaatccaaa
    17701 gtaattaaaa caataccatt gcaaaaccat atcgataata cgactcaaca gcatgaacaa
    17761 gcattaagct gggtaattaa aaatgaaaga caagctggtg caacaacaga tacatttcca
    17821 ggcattaata aatggttgat accaattggc acatctccga ttaaaggcat tctagccatt
    17881 gattaccaaa gttctcaagt gattaatcca tatgatgcat caatattaga atcaatgtta
    17941 aatgaacttt ctctcgccgt tgaaaatgtg acgttgctta aacaaacgag agaatccatg
    18001 ttacaggctg aacgtcaact gacacattca aattttttaa gatcaatttc gcatgacata
    18061 cgtacaccgt taacaacaat tatggggaat ttagatattt tggtatcaca tagtaaagat
    18121 atgtctatca tagaaaagga acaattactt gtgcatagtt ttcaagaaag tcagtattta
    18181 tatctattag taacaaatat tttatcatta acaaaattac agtcatcaaa tgttcagata
    18241 aaattgcaac catatctcgt tagtgaatta gtagaagaaa tcgatatgat tttagaacgt
    18301 cgccatttaa aaaagcgtat aactgtatca tcctcagtag atttacagtt tatacacatt
    18361 gacagtaagt tgattttaca agctttattc aatttgattg aaaatgcagt taaacacact
    18421 tctactgaca ccaaaatcaa tttatctatt cgttatgcta gcgatgaaca aatcgaattt
    18481 gccgttatag acgaaggacc tggaattagt ttaaaagaac aacaaaagat attcgagcct
    18541 ttttacacag gatcaaataa atattttaaa gataaccaaa aagaaagcat gggactaggc
    18601 ttatatttag tacagactat cctacacaaa catcaatcaa acctacaata taaaccgaac
    18661 caaccacatg gcagtatatt ctatttcaat atagatacag actttaatga aggagacata
    18721 taatgcaatc taaaatattg ataattgaag atgatcacgc aatcacacat ttacttgatg
    18781 ttgcattaac tttagattat tacaatgtaa ctacagccga caatgccaca caagcacact
    18841 ttaaaattca aattgataaa ccagatgtca ttttattaga tttaggttta ccagataaag
    18901 atggattatg tttgatttca gaaatcaggc aacatactga cattcctatc attgtaataa
    18961 gtgcaagaca agaagaacaa acaattattc aagctttaga taacggtgcg aatgactata
    19021 tgactaagcc ttttaatgtt gatgagcttc gggcacgaat tcgagtcata gagagaatcg
    19081 ctaagtcaca tcaagaaact aactttgtat ttactaacgg tttgctaagc atcgactttg
    19141 gctccaaatc tgttgttatt aataatcagg aggtacattt gacaccaaat gaatttagct
    19201 tgttagaact attgtcaaat cataaaggaa aagtactcac ttatgagatg atattaaaac
    19261 gaatatatgg ctatgttaat aagactgaaa tgcctagttt aagggtgcat atgacatcat
    19321 taagacaaaa actttcacaa tgtcatgagg acgcaaaaga tattattaaa acacatccac
    19381 gcattggtta tcaaatgttg cagtggaaag agaaataatt aaataaaaaa agatcgctgc
    19441 cattaaacga atgattggca gtgatctttt ttatattcaa cttattattt atcgcgcact
    19501 agtgtcaaca aaatgcccta ttgtttttta caagaacatg atactgaaat ttcataaagt
    19561 ttatttatgt tcgattattc ttaatttaag tagttgttat attctagatt catatttata
    19621 tttaatcaga atgatattac cgcacacagc tctcaattca attaaatatt taacctttaa
    19681 aacgttagta ttttgatatc tacagagaat cagttattga aatcaaaaaa gagctcaaga
    19741 cattacatct tcaagctctt aatctataaa ttatttttga tggtcagcaa atgtgcgacc
    19801 tttcataggt ctgcttccac cgctgctctt ctctttacgg tcgaattttt ttgtactttt
    19861 cttcttactt gagtatcctt ttgaacgttt actcttactg tcaaatttag gattaccacg
    19921 cttactattt ctgttacgag aaccgcttgg tttaccgtta cggcctttgc gagataatgg
    19981 tttttcaaaa gttaattgaa cttcaacttc atcgttcgct tctactaact cttgtaaaag
    20041 tgcagcaact aaatcaacat cgttatattc atttaacaac tctgtagaaa tgcgtttcaa
    20101 acgtgattct gagtctttag acatccagtt ttcaactttt tctttgatgt catcttcacg
    20161 tgcttgaagt acttctttac gatgtggtgg acgaagtgcg ctcatttttc taccattagc
    20221 atcttcaatt tgtctaatgt aatccatttc aattggattt acaaatgtta ccgcgatacc
    20281 ttctttacca gcacgacccg tacgaccaat acggtgtgta tagctttcag tatcttgagg
    20341 tatatcaaag ttataaacat gactcacacc agaaatatct agtcctcttg ctgctacatc
    20401 agtagcgact aaaatattaa tttggtcatt tttaaatttc tttaatactt ctaaacgttt
    20461 cgcttgtgta atatcaccat gtaagccttc agctttatat cctttagaaa tcaaagcact
    20521 tgttaattca tcaacacgac gttttgtacg tccgaatacg attgctaatt caggttgatg
    20581 aacatctagg aaatttgtaa atgtatcaaa tttctctaat tctttaacaa ttgtatagaa
    20641 ttcttcgatt tgtggatcag acatttcatt attcattgtc ttaatgattt ttggtgattt
    20701 cataaattgt tgtactaaag cttggattgc tttaggcatt gtagctgaga acaacattgt
    20761 ttgacgttgt actgctggaa ttttatccat aataaatctc atatcatcga tgaatcccat
    20821 attcatcatt tcatcagctt catctaaaat caaagtatga attccgtccg tttttaatgt
    20881 gcgacgattt aaatggtcga taacacgccc aggtgttccg actacgattt gtgggccctt
    20941 tttcaaggct ttaatttggc gttcgatagg cataccaccg aatacagtaa caacttggac
    21001 accttgtcca cggctaaatt ctcttaattg ttcagctacc tgcattgcca attctcttgt
    21061 aggtgctaaa attaacgatt gaaccccttg tttccctact actttctcaa ttaaaggaat
    21121 accgaatgct cctgttttac ctgtaccggt ttgagcttgc ccaaggatat caattccttg
    21181 taacgcataa gggatactgt ctttttggat aggtgtcggc tctttaaatc ccattgattc
    21241 aagtgactga accgtattat ccgaaatccc tagttcttta aaattttgca aaataattct
    21301 cctttttacc tgtttatttc tcaatatact tttgcatata agttcattaa tatttcatca
    21361 acttctatat agtaccacca aaaaaattta atcgcaataa agcaatttgc gaatatttat
    21421 tagaatcaca ttaattgatt gtcatatttt tgtcaaaaag ctagcattag ataagatgta
    21481 agcgttacgc atcattcgaa taatcattat aaaaaatttt ttataaaata cgtatatcgt
    21541 taacattcca caacaaattt tactgaaaca catgtgaaat tagacattat aatacaacat
    21601 ctttttgtaa aatattatat aaacattcaa actacttcct atataaagtg tgtacataga
    21661 attaaccttg atttagtttc aaatttatag atttaacata aaaagaaaaa ggcaattttg
    21721 tatttacaca caaaaccacc tcttctttgt aatcttttag tattaattca aatcattttc
    21781 taagtaaaag gtcgctcgac taatctctat gaaattaaag catttacgac ttcttctaac
    21841 ttcataccac gtgagccttt aactaataca cggtcatgcg cttttaaatc acttgttaaa
    21901 acttctatca tatcgtcttt agaattgaag tgttgtgctt tttcgacatg ttgctggccc
    21961 gaatcataaa tatacttcgc ttcattacca aacgtataca acacatctat atgcttttct
    22021 tctaaataat tacctacacc gatatgcatt tctttgctat tttcacctaa ttctaaaaca
    22081 tctcccaaaa ttagaatgcg acgccctgtc aaagtactca gtgtatcaat agctgctctc
    22141 atacttgtag gacttgcatt ataggcatca tttatcacag tgatatcatt ttctaatgta
    22201 tgttgctcca tacgcatacc agttaagctg acatttttta aattttgata gattatgtta
    22261 tatgtcaaac ctaattcatg accaaccgca atggcaatcg tcgcattttt catattatgc
    22321 tttcctaata ttggcagatc gtaatgttca ttattaatcg taaatgaaat acctgtagta
    22381 tctctatcat caacagaaca aactaatgca ttatcagtag caacaccaat actaatacat
    22441 tttgcatttt caacttcttt aacatgcggt tttaataatg gttcatcgcc atcatatata
    22501 aacgtaccat tatcttttag acctattgta atttcagatt tagctttagc aatcccctca
    22561 cgcgaaccta aatcttgcat atgtgactca ccaatattag ttatgactgc aatatctggt
    22621 tgagcgaggt ttgacagaaa ttcaatttca tggaaacctg acatccccat ctccaatatt
    22681 gatatttcag tatcattatc taattccaaa atagttaaag gtaaaccaat ttcattattg
    22741 taattacctt gcgttttctt aactttaaat tcggtatgca atacactttc aatcatatct
    22801 ttagtcgttg ttttaccatt agaccctgtg acggcaatta ctttagggtt tacatgtctc
    22861 aagtaagctt gtgccaattg ttgtaatgcc gttaatgtgt cttcaaccca tataataggc
    22921 ccgcttacat tttcatctat aggtgtgcct ttttgataaa aagcagcccc agcaccatct
    22981 tgtaatgcct tagagacaaa gcgatgaccg tcaacatttt cacctttaaa tggtataaat
    23041 aacatatttt tagaaattgc tcgtgaatca attgtgactc catttatctc ttgatttaaa
    23101 aattgatctt caatttcaca aggaatccat gattgaattt gctttaatgt aacattaatc
    23161 ataataacaa cctcagttag tcaattttgt atttattttt ctgtttatcc tggtgacgtt
    23221 ctttagcaag ctcgataagt tttgtaatca attctggata agataagccc atattttccc
    23281 ataattttgg atacatactg aaagccgtaa atccaggcat tgcatttgtt tcattaatat
    23341 atatttggtt gtcttctgtt acaaagaaat cagcacggac taaaccagaa caatctgtcg
    23401 ctttgaatgc ctctaatgcc atatttctaa gcgttaattg aacatcttcg tctaagtcag
    23461 ctggaatttg taattgaacc ttaccatctt tatattttga tttgtaatcg taaaacgcga
    23521 catcttttac gacttcacct ggccatgtcg cttcaggata gtcatttcct aaaactgcta
    23581 cttcaatttc acgtgcgtta acgccttgtt caataacaag cttacggtca aattggaatg
    23641 cttctttaat accttcttta agttccgctt cattattaca tttactgata cctacacttg
    23701 accctaagtt agcaggttta acaaagactg ggtaatttaa tttatcattt actaatttta
    23761 aaatgttatg ttcatatttt tcatattcag aacgtaagaa actaatataa ggtaactgtg
    23821 gtaaccctcg atgttcaaat aattgtttca ttacaagttt gtccatagaa cttgcagctg
    23881 acaatacacc atttcctaca tatggtacat ccaatacttc aaaaagccct tgaatcgtgc
    23941 catcttcacc attaggacca tgtaataatg ggaatactgc atcgtatggt tgtcctgaac
    24001 tactttcttt cagtagctgt gaaatctcaa gcgcctctcc attttctaaa tgaagctcat
    24061 cagtagattt aatttcagtt gtaatattat tttgctttct ccaatcacca tcattggtaa
    24121 tataaatgat atcaacatga tatttgtctt tatctattgc atttaataca ttttgtgctg
    24181 tcagaatcga tacttcgtgt tctgcacttt tccctccaaa aacgatacaa atattttctt
    24241 ttgtcatttc gttttcctcc aatgatatat cagggtttgc taccttaata taaattaata
    24301 ttgatttagt ccaattgtta aatgatttat tcgcatcttt gaatcttaat agtatcacat
    24361 tttaatgctg ttttgcattt taaaaacgag gcgttatgtg tctgtatcta tgttgattca
    24421 tttataatca gaacaatatt cccttctatt ttattcctaa ttcaaattga aatctatagc
    24481 tgatatcact gtgatattct agctaatttt taaaaagtca tgtaaaattg atataaacat
    24541 tagtgagtat aaaaggagtt tgcaatgaat tattcatctc gtcaacagcc ggataagcat
    24601 tggcttcgca aagtagactg ggtattagta gccactatag ctgttttagc aattttcagt
    24661 gttctgctta ttaactcggc aatgggcggt ggtcaataca gtgctaattt cggtatcaga
    24721 caaatttttt attacatttt aggtgcaatt tttgcaggta tcatcatgtt tatttcacct
    24781 aaaaagatta aacattatac atatttattg tatttcttaa tctgtctatt attaatagga
    24841 ttgctcgtta ttcctgagtc acctattaca cctattatca atggtgccaa aagttggtac
    24901 acgtttggcc ctatcagtat tcagccatct gaattcatga aaattatttt aattttagca
    24961 ttagcgcgtg tcgtttctag acataatcaa ttcacattca ataaatcatt ccaaagtgat
    25021 ttgttattat ttttcaaaat tattggtgtc tcgttagtac caagcatttt aatattactg
    25081 caaaatgacc taggaactac attagtatta gctgcaatta ttgcaggtgt gatgttagta
    25141 agtggtataa catggcgtat cttagcacct atctttatta caggtattgt tggtgcaatg
    25201 acagtcattt taggcattct atatgcaccc gcattaattg aaaatttatt aggtgtccaa
    25261 ctgtatcaaa tgggacgaat caattcatgg cttgacccct atacatatag tagtggtgat
    25321 ggctatcatt taactgaatc acttaaagct atcggttcag gacagttact aggtaaagga
    25381 tacaatcacg gtgaggttta tatacctgaa aatcatactg actttatctt ttcagtgatt
    25441 ggagaggaac ttggttttat cggttctgtc atattgatct taatattttt atttttaatc
    25501 ttccatctaa taagattagc tgcgaaaatt gaagatcaat ttaacaaaat ctttatcgtt
    25561 ggtttcgtca ctttacttgt gttccatatt ttacaaaata ttggtatgac aattcagttg
    25621 ttaccaatca ctggtattcc attaccattt attagttatg gtggtagtgc gctatggagt
    25681 atgatgactg gaataggtat agtcttatca atttattatc atgaaccaaa acgatatgta
    25741 gatttatacc atccaaaaag taattaat
//
LOCUS       DAPIDM010000023        25473 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000023 DAPIDM010000000
VERSION     DAPIDM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.023"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            4..315
                     /locus_tag="RJE42_000875"
     CDS             4..315
                     /locus_tag="RJE42_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV5876319.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            415..1272
                     /locus_tag="RJE42_000876"
     CDS             415..1272
                     /locus_tag="RJE42_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDV5876320.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            1301..1897
                     /locus_tag="RJE42_000877"
     CDS             1301..1897
                     /locus_tag="RJE42_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDV5876321.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEETGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            1918..5742
                     /locus_tag="RJE42_000878"
     CDS             1918..5742
                     /locus_tag="RJE42_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV5876322.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYRRDYRKQSHDSRLQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNI
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKSSEKVESDKQ
                     KYDKYVAETQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSTPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTVASQHHTNGIENEAVSNDENKQASIQNEDTNDAHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSDVTSQRQSALQHNVEVNDQDGLKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNSQDNTNRNASEMHVDVPKTQEH
                     AVTESQVNNIDKTVDNDIELAPRHKKDNQTNLSVNSLKTNDVNDGHVVEDSTMNEIEK
                     HSAEVTENVQNEAAESEQNGEEKTIENANPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPIQSKQAVSESKTASQATPSSSTDSQESNTNAYKTNNMTSNNVE
                     NDQLIGHGETENDYQNAQQYSDQKPSVDSTQTEIFEESQDDNQLENEQVDQLTSSSVS
                     EVSDITEESEETTHLNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQPDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            5766..7079
                     /locus_tag="RJE42_000879"
     CDS             5766..7079
                     /locus_tag="RJE42_000879"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV5876323.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKESDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINALEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            7153..7644
                     /locus_tag="RJE42_000880"
     CDS             7153..7644
                     /locus_tag="RJE42_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDV5876324.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            7718..9070
                     /locus_tag="RJE42_000881"
     CDS             7718..9070
                     /locus_tag="RJE42_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876325.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAETDKSAEVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKREVQTETDKSAEVSNKESKAAALKAQQAAIKEEAS
                     ANNLSDTSQEAQAIQEAKREVQTEKDSDTSTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSEKGNTDNKDTVKQKEA
                     KFENGVITRKADEKTTDNTDVDKKSGKQSKKTTPSNKRNASKASTNKTSSQKKQHNKK
                     ASQGAKKQSSSSNSTQKNNQTSNKNSKTTNAKSSNASKKSNAKVEKAKSKIEKRTFND
                     "
     gene            9226..9309
                     /locus_tag="RJE42_000882"
     CDS             9226..9309
                     /locus_tag="RJE42_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876326.1"
                     /translation="MLFETSLDNNSDIAHFKLMMEKYLIIK"
     gene            9764..10855
                     /locus_tag="RJE42_000883"
     CDS             9764..10855
                     /locus_tag="RJE42_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDV5876327.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
     gene            11209..11322
                     /locus_tag="RJE42_000884"
     CDS             11209..11322
                     /locus_tag="RJE42_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876328.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISGFSLNFSKQKYVTVN"
     gene            11395..12384
                     /gene="ccpA"
                     /locus_tag="RJE42_000885"
     CDS             11395..12384
                     /gene="ccpA"
                     /locus_tag="RJE42_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDV5876329.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLAGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(12482..13651)
                     /locus_tag="RJE42_000886"
     CDS             complement(12482..13651)
                     /locus_tag="RJE42_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDV5876330.1"
                     /translation="MQQHLSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNTNL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HTHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFNLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADTYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(13676..14308)
                     /locus_tag="RJE42_000887"
     CDS             complement(13676..14308)
                     /locus_tag="RJE42_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876331.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDVHLTAFRD
                     SEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            14477..16183
                     /gene="acsA"
                     /locus_tag="RJE42_000888"
     CDS             14477..16183
                     /gene="acsA"
                     /locus_tag="RJE42_000888"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV5876332.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            16624..18291
                     /locus_tag="RJE42_000889"
     CDS             16624..18291
                     /locus_tag="RJE42_000889"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDV5876333.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(18536..18625)
                     /locus_tag="RJE42_000890"
     CDS             complement(18536..18625)
                     /locus_tag="RJE42_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876334.1"
                     /translation="MLLTTPIRWYLIDNNYQYQLLVLIVAHPN"
     gene            18624..21311
                     /gene="harA"
                     /locus_tag="RJE42_000891"
     CDS             18624..21311
                     /gene="harA"
                     /locus_tag="RJE42_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDV5876335.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPTNTAKTYPAADESLKDAIKNPAVENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGAETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVASNDQSSSDASNQTNTNTSNQNTSTTNNASDQPQATTN
                     MSQPAKPVSSANADQASSQPAHETNSNGNTNDKTNASSNQSNGNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGAREVKIVSSIEYGENFHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQDKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYVS
                     DKAIYNAIVKVVVANIGYEGQYHVRIVNQDIKTKDDDTSQNNTSEPLNVQTGQENKVS
                     ATDTAENSSTATNPKDASDKADVIEPESDVVKVTDSNIDKDAHHDVDHLSDMSDNTHL
                     DKYDLKEMDTQIAKDTDKGVDKDADNSVGMSSNVDTEKDINKNEGKVIQLAHNTDKNN
                     HTGKAAKLDGVRQNYNNIDKVTDKKTTEHPSNDIHKTVDKTVKTKEKSGTPSKENKLS
                     QSKMLPKTGETTSSQTWWGLYALLGMLALFIPKFRKEPK"
     gene            21445..21537
                     /locus_tag="RJE42_000892"
     CDS             21445..21537
                     /locus_tag="RJE42_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876336.1"
                     /translation="MKIILLLFLIFGCIVMVSLKSEHQLTLFSI"
     gene            complement(21517..22422)
                     /locus_tag="RJE42_000893"
     CDS             complement(21517..22422)
                     /locus_tag="RJE42_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDV5876337.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTNQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            22803..24065
                     /locus_tag="RJE42_000894"
     CDS             22803..24065
                     /locus_tag="RJE42_000894"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV5876338.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(24159..25433)
                     /locus_tag="RJE42_000895"
     CDS             complement(24159..25433)
                     /locus_tag="RJE42_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDV5876339.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDSEDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
BASE COUNT         9463 a         3678 c         4653 g         7679 t
ORIGIN      
        1 aaaatgaaac aacttgaatc agaacaacaa tttgaatctt taaaacaagg tgctacagta
       61 tttgaattca ctgcaggctg gtgtccagat tgtagagtga tagaaccaga tttaccggaa
      121 ttagaagcga gatatcctat gtttgacttc gtatcagtag accgtgataa atttatggat
      181 atttgtattg aaaatggtat tatggggatt ccaagttttc tagtatataa aaatggagaa
      241 ctgcttggaa gttatattgg aaaagaacga aaatcaattg aacagataga tgcattttta
      301 gctcaatacg tgtaatttag actagagaaa aacggggtaa tactcgtttt tctctgttac
      361 tatgtgttga tttattgtaa actattataa ggtgcgaaat taggagtgtt acatatgaat
      421 acctttcaaa tgagagataa attaaaggaa cgtttaagcc atttagacgt tgattttaaa
      481 tttaatcgtg aagaagaaac tttgcgtatt tatcgaacag ataataacaa aggtatcacg
      541 attaaactta acgctatagt cgcaaaatat gaagataaaa aagaaaaaat tgtagatgaa
      601 attgtttatt acgttgatga agctattgca caaatggcag ataaaaccct tgagagtata
      661 tcatctagtc aaattatgcc tgtcattaga gcgactagct ttgataaaaa aactaaacaa
      721 ggtgttcctt ttatctatga tgagcatact gcagaaacag cagtttatta cgcagtcgat
      781 ttagggaaat catatcgtct aattgacgaa agcatgttag aggatttgaa gttaactgaa
      841 caacaaataa gagaaatgtc tctgtttaat gttagaaaat tgtcaaattc atatacgact
      901 gatgaagtaa aaggcaatat tttttatttt attaactcaa atgacgggta tgatgcaagt
      961 aggatactaa atactgcatt tttaaatgaa attgaggcac aatgtcaagg cgaaatgctc
     1021 gtagcagtgc cacaccaaga tgtgttaata attgcagata tacgcaataa aacaggatat
     1081 gatgtgatgg cacatttaac aatggaattt ttcactaaag gtctagttcc aattacatca
     1141 ttatcctttg gatataaaca gggtcatctt gaaccgatat ttattttagg taaaaacaat
     1201 aaacaaaaaa gagatccaaa cgtgattcag cgtttagaag caaatcgtcg taaatttaat
     1261 aaagataaat agaaataatt ggataaggag tttggtcata atgaatttat tttacaatcc
     1321 taaatatgta ggagatgtcg catttttaca aattgaacca gttgaaggtg aattaaacta
     1381 caataaaaaa ggtaacgttg ttgaaattac taatgaaggt aatgttgtag gttataatat
     1441 ttttgaaatt tcaaaagata taacaattga agaaacaggt catattaaat taactgatga
     1501 acttgtaaat gtattccaaa agcgtatttc agaagctggt tttgattata aattaaatgc
     1561 tgatctatca ccgaaatttg tagttggcta cgttgaaact aaagacaaac atcctgatgc
     1621 agataaatta agtgtactaa atgtaaatgt tggaaatgac acattacaaa ttgtatgtgg
     1681 cgcgcctaac gttgaagctg gacagaaagt tgttgttgct aaagtaggtg cagtgatgcc
     1741 tagcggtatg gtaattaaag atgctgaatt acgtggtgtt gcctcaagcg gtatgatttg
     1801 ttcaatgaaa gaattgaatt tacctaatgc acctgaagaa aaaggtatta tggtattaaa
     1861 tgacagctat gaaattggac aagcattttt tgaataatta aggaaggtag tgaaaatatg
     1921 agctggtttg ataaattatt cggcgaagat aatgattcaa atgatgactt gattcataga
     1981 aagaaaaaaa gacgtcaaga atcacaaaat atagataacg atcatgactc attactgcct
     2041 caaaataatg atatttatag tcgtccgagg gggaaattcc gttttcctat gagcgtagct
     2101 tatgaaaatg aaaatgttga acaatctgca gatactattt cagatgaaaa agaacaatac
     2161 cgtcgagact atcgcaaaca aagccacgat tctcgtttgc aaaaacgaca tcgccgtaga
     2221 agaaatcaaa caactgaaga acaaaattat agtgaacaac gtgggaattc taaaatatca
     2281 cagcaaagta taaaatataa agatcattca cattaccata cgaataagcc aggtacatat
     2341 gtttctgcaa ttaatggtat tgagaaggaa acgcacaagt caaaaacaca caatatatat
     2401 tctaataata caaatcatcg tgctaaagat tcaactccag attatcacaa agaaagtttc
     2461 aagacttcag aggtaccgtc agctattttt ggcacaatga aacctaaaaa gttagaaaat
     2521 ggtcgtatcc ctgtaagtaa atcttcagaa aaagttgagt cagataaaca aaaatacgat
     2581 aaatatgtag ctgagacgca aacgtctcaa aataaacaat tagaacaaga gaaacaaaat
     2641 gatagtgttg tcaaacaagg aactgcatct aaatcatcag atgaaaatgt atcatcaaca
     2701 acaaaatcaa cgcctaatta ttcaaaagtt gataatacta tcaaaattga aaatatttat
     2761 gcttcacaaa ttgttgaaga aattagacgt gaacgagaac gtaaagtgct tcaaaagcgt
     2821 cgatttaaaa aagcgttgca gcaaaagcgt gaagagcata aaaacgaaga gcaagatgca
     2881 atacaacgtg caattgatga aatgtatgct aaacaagcgg aacgctatgt tggtgatagt
     2941 tcattaaatg atgatagtga cttaacagat aatagtacag tggctagtca gcatcataca
     3001 aatggaatag agaatgaagc tgtatcaaat gatgaaaata aacaagcgtc aatacaaaat
     3061 gaagacacta atgacgctca tgtagatgaa agtccataca attatgagga agttagtttg
     3121 aatcaagtat cgacaacaaa acaattgtca gatgatgaag ttacggtttc ggatgtaacg
     3181 tctcaacgtc aatcagcact acaacataac gttgaagtaa atgatcaaga tggactaaaa
     3241 aatcaatcca gattaattgc tgattcagaa gaagatggag caacgaataa agaagaatat
     3301 tcaggaagtc aaatagatga tgcagaattt tatgaattaa atgatacaga agtagatgag
     3361 gatactactt caaatagcca agataatacc aatagaaacg cgtctgaaat gcatgtagac
     3421 gttcctaaaa cgcaagagca cgcagtaact gaatctcaag ttaataacat cgataaaacg
     3481 gttgataatg acattgaatt agcgccacgt cataaaaaag ataatcaaac aaacttaagt
     3541 gtcaactcat tgaaaacgaa tgatgtgaat gatggtcatg ttgttgaaga ttcaaccatg
     3601 aatgaaatag aaaagcatag cgcagaagtt acagaaaatg tgcaaaacga agcagctgaa
     3661 agtgaacaaa atggcgaaga gaaaactatt gaaaacgcaa acccaaagaa acagactgaa
     3721 aaggtttcaa ctttaagtaa aaggccattt aatgttgtca tgacgccatc tgataaaaag
     3781 cgtatgatgg atcgtaaaaa gcattcaaaa gtaaatgtgc ctgaattaaa gcctatacaa
     3841 agtaagcaag ctgtgagtga aagcaagact gcgagtcaag ccacaccatc atcaagcact
     3901 gattcacaag agtcaaatac aaatgcatat aaaacaaata atatgacatc aaacaatgtt
     3961 gagaacgatc aacttattgg tcatggagaa acagaaaatg attatcaaaa tgcacaacaa
     4021 tattcagatc agaaaccttc tgttgattca actcaaacgg aaatatttga agagagccaa
     4081 gatgataatc aattggaaaa tgagcaagtt gatcaattaa cttcgtcttc agtttcagaa
     4141 gtaagcgaca taactgaaga aagcgaagaa acaacacatc taaacaatac tagtggacaa
     4201 caagataatg atgatcaaca aaaagattta cagccttcat tttcaaatca aaatgaagat
     4261 acagctaatg aaaatagacc tcggacgaat caaccagatg ttgcaacaaa tcaagctgta
     4321 caaacatcta agccgatgat tcgtaaaggc ccaaatatta aattgccaag tgtttcatta
     4381 ctagaagaac cacaagttat tgagccggac gaggactgga ttacagataa aaagaaagaa
     4441 cttaatgacg cactatttta ctttaatgtt cctgcagaag tacaagatgt aactgaaggt
     4501 ccaagtgtta caagatttga attatcagtt gaaaaaggag ttaaagtttc aagaattacg
     4561 gcattacaag atgacattaa aatggcattg gcagcgaaag atattcgtat agaagcgcca
     4621 attccaggaa ctagtcgtgt tggtattgaa gttccgaacc aaaatccaac gacggtcaac
     4681 ttacgttcta ttattgaatc tccaagtttt aaaaatgctg aatctaaatt aacagttgcg
     4741 atggggtata gaattaataa tgaaccatta cttatggata ttgctaaaac gccacacgca
     4801 ctaattgcag gtgcaactgg ctcagggaaa tcagtttgta tcaatagtat tttaatgtct
     4861 ttactatata aaaatcatcc tgaggaatta agattattac ttattgatcc aaaaatggtt
     4921 gaattagctc cttataatgg attgccacat ttagttgcac cggtaattac agatgtcaaa
     4981 gcagctacac agagtttaaa atgggccgta gaagaaatgg aaagacgtta taagttattt
     5041 gcacattacc atgtacgtaa tataacagca tttaacaaaa aagcaccata tgatgaaaga
     5101 atgccaaaaa ttgtcattgt aattgatgag ttggctgatt taatgatgat ggctccgcaa
     5161 gaagttgagc agtctattgc tagaattgct caaaaagcga gagcatgtgg tattcatatg
     5221 ttagtagcta cgcaaagacc atctgtcaat gtaattacag gtttaattaa agccaacata
     5281 ccaacaagaa ttgcatttat ggtatcatca agtgtagatt caagaacgat attagacagt
     5341 ggtggagcag aacgcttgtt aggatatggc gatatgttat atcttggtag cggtatgaat
     5401 aaaccgatta gagttcaagg tacatttgtt tctgatgacg aaattgatga tgttgttgat
     5461 tttatcaaac aacaaagaga accggactac ctatttgaag aaaaagaatt gttgaaaaaa
     5521 acacaaacac aatcacaaga tgaattattt gatgatgttt gtgcatttat ggttaatgaa
     5581 ggacatattt caacatcatt gatccaaaga catttccaaa ttggctataa tagagcagca
     5641 agaattatcg atcaattaga gcaactcggt tatgtttcga gtgctaatgg ttcaaaacca
     5701 agggatgttt atgttacgga agcagattta aataaagaat aattatgagt aaggagtttt
     5761 atataatgac acactatcat tttgtcggaa ttaaaggttc tggcatgagt tcattagcac
     5821 aaatcatgca tgatttagga catgaagttc aaggatcgga tattgagaac tacgtattta
     5881 cagaagttgc tcttagaaat aaggggataa aaatattacc atttgatgct aataacataa
     5941 aagaagatat ggtagttata caaggtaatg cattcgcgag tagccatgaa gaaatagtac
     6001 gtgcacatca attgaaatta gatgttgtaa gttataatga ttttttagga cagattattg
     6061 atcaatatac ttcagtagct gtaactggtg cacatggtaa aacttctaca acaggtttat
     6121 tatcacatgt tatgaatggt gataaaaaga cttcattttt aattggtgat ggcacaggta
     6181 tgggattgcc tgaaagtgat tatttcgctt ttgaggcatg tgaatataga cgtcactttt
     6241 taagttataa acctgattac gcaattatga caaatattga tttcgatcat cctgattatt
     6301 ttaaagatat taatgatgtt tttgatgcat tccaagaaat ggcacataat gttaaaaaag
     6361 gtattattgc ttggggtgat gatgaacatc tacgtaaaat tgaagcagat gttccaattt
     6421 attactatgg atttaaagag tcggatgaca tttatgctca aaatattcaa attacggata
     6481 aaggtactgc ttttgatgtg tatgtggatg gtgagtttta tgatcacttc ctatctccac
     6541 aatatggtga ccatacagtt ttaaatgcat tagctgtaat tgcgattagt tatttagaga
     6601 agctagatgt tacaaatatt aaagaagcat tggaaacgtt tggtggtgtt aaacgtcgtt
     6661 tcaatgaaac tacaattgca aatcaagtta ttgtagatga ttatgcacac catccaagag
     6721 aaattagtgc tacaattgaa acagcacgaa agaaatatcc acataaagaa gttgttgcag
     6781 tatttcaacc acatactttc tctagaacac aagcattttt aaatgaattt gcagaaagtt
     6841 taagtaaagc agatcgtgta ttcttatgtg aaatttttgg atcaattaga gaaaatactg
     6901 gcgcattaac gatacaagat ttaattgata aaattgaagg tgcatcgtta attaatgaag
     6961 attctattaa tgcattagaa caatttgata atgctgttat tttatttatg ggtgcaggtg
     7021 atattcaaaa attacaaaat gcatatttag ataaattagg catgaaaaat gcgttttaat
     7081 atgtttatac tagagtagta tgggtattta ttattaatga cattattaca tgttaattag
     7141 gaggcgtttt taatggattg gattttacca attgctggaa ttatcgctgc gattgcattc
     7201 ttaattttat gtatcggtat cgtagctgta ttaaattctg ttaagaaaaa cttagattat
     7261 gttgcaaaaa cacttgacgg tgtagaaggt caagttcaag gtattactcg tgaaacaaca
     7321 gatttacttc ataaagtaaa ccgtttaact gaggatatcc aaggtaaagt agatcgttta
     7381 aactcagttg tagatgctgt taaaggtatc ggtgactcag tacaaacgtt aaacagctct
     7441 gtagatcgtg taacaaattc aattacacat aatatttctc aaaatgaaga taaaatctca
     7501 caagttgttc aatggtcaaa tgttgcaatg gaaattgcag acaaatggca aaatagacac
     7561 taccgtcgtg gaagtgcaaa ttacaaagct aataatgtag caactgatgc aaatcatagc
     7621 tatacttctc gagtagataa ataattaata attaaacaaa tgcacttaaa tgaggttgtt
     7681 acttatttaa gtgcatttgt tataggagga tatacaaatg acgcaacaac aaaataataa
     7741 aagaacatta aaaaataaac acacttatca aaatgaacca ttaccaaacc gtaaagattt
     7801 tgttgttagt tttataactg gcgcgcttgt tggttcagct ttaggcttat attttaaaaa
     7861 taaagtttat caaaaagcag atgatttaaa agtcaaagaa caagaactgt cgcaaaagtt
     7921 tgaagaaaga aaaacgcaac ttgaagaaac ggttgccttt acaaaagaac gtgttgaagg
     7981 atttttaaac aaatctaaaa atgaacaagc ggcattgaag gcacaacaag cagcaataaa
     8041 agaagaagca agtgcaaata atttaagtga tacatcacaa gaggcacaag aaattcaaga
     8101 agctaaaaga gaagcacaag cagaaacgga taaaagtgca gaagtatcaa ataaagaatc
     8161 aaaagcagtg gcattgaaag cacaacaagc agcgataaaa gaagaagcaa gtgcaaacaa
     8221 tttaagcgat acatcacaag aggcacaaga aattcaagaa gctaaaagag aagtacaaac
     8281 agaaacagat aaaagtgcag aagtatcaaa taaagaatca aaagcagcgg cattgaaggc
     8341 acaacaagca gcgataaaag aagaagcaag tgcaaataat ttaagtgata catcacaaga
     8401 ggcacaagcg attcaagaag ctaaaagaga agtacaaaca gagaaagaca gtgacacatc
     8461 aactaaagat gcaagtgcag caaaggtaga agtatcaaaa ccagaatcac aagctgaaag
     8521 attagcgaac gctgcaaaac agaagcaagc taaattaaca ccaggttcaa aagagagtca
     8581 attaactgaa gcgttatttg cagaaaaacc agttgctaaa aatgacttga aagaaattcc
     8641 tcaattagtt actaaaaaga atgatgtatc agaaaaaggt aatacagata ataaagacac
     8701 tgtaaaacaa aaagaagcta aatttgaaaa tggtgttatt acacgtaaag ctgatgaaaa
     8761 aacaactgat aatacagatg ttgacaagaa atcaggtaaa caatctaaaa aaacaacacc
     8821 ttcaaataaa cgaaatgcat caaaagcatc tacaaataaa acttcaagtc agaaaaagca
     8881 acataataag aaagcatcac aaggtgcaaa gaaacagagt agttcaagta attcaactca
     8941 aaagaataat caaactagta ataagaattc aaaaacaaca aatgctaaat catcaaatgc
     9001 atcaaaaaaa tcaaatgcaa aagttgaaaa agctaaaagt aaaatagaga aacgtacatt
     9061 taatgactaa gcatactaaa atcgtttaat aatcaatcat accattcagc tatgtattat
     9121 atttttaaaa attgaatttc aaaataaaag ctaggcattc aggtgcttac cttttttgat
     9181 atcatcagtt tattttgaaa ggcaacgcga tcatttcatg tatttatgtt gtttgaaaca
     9241 tctttagata acaatagtga tattgcacat tttaagctga tgatggaaaa atatttgata
     9301 atcaaataaa taatgaatct aagaaggtac aatgacgttc actaattcaa atatagctta
     9361 atatgttggt gagtgatgtt cactatagat ttatattaat atacaaaggt tgttaatttt
     9421 tattaagcgt taggttgaat gtatgagaat tttagattta taatagaaga tagaaaccaa
     9481 aatttttctt aaaagctgta atgttgactc aaaataagct ataatcatga cacttattta
     9541 attgattaac atgtgctaat aaatatcaat atagaatata actttcaaac aatgactgag
     9601 aaaatcgaaa tgtcagtcaa gcaccatata gttagaaaat taattatttt ctgtcaattt
     9661 agggttgaac tatacatatg atattgttag aatatttttt aacattatat tttattgctt
     9721 taaagtggaa tatacttgaa ataattcgta gaggtgagta aggatgagta ataaattaga
     9781 atcatacaga agtgagattg tctcactgaa tcatcaaatt ttagacttat tatctaaacg
     9841 tggtgaacta gcacaaaaaa ttggggaaga aaaattaaaa caaggtacac gtatctatga
     9901 tccacaacgt gaaaaagaaa tgcttaacga cttaatcgat agtaacaaag gaccattcaa
     9961 cgataatact attaagcaat tatttaaaga aattttcaaa gcctctacag atttacaaaa
    10021 atctgaaaat gaaaaacatt tatatgtatc acgtaagttg aaacctgaag atacgattgt
    10081 aacatttgat aatgggggca ttattggaga cggcaataaa tcatttgtat ttgggccatg
    10141 ttcagttgaa tcatttgaac aagttgaagc tgttgctaaa aacttacatg ctaaaggtga
    10201 aaaatttatt cgtggcggtg catttaaacc acgtacatca ccatatgatt tccaaggttt
    10261 aggtgttgaa ggtcttaaga tacttaaaca aattaaagat aaatatgatt taaatgttgt
    10321 cagcgaaatc gtaaatccaa atgattttga agtggctgat gagtatttag acgtattcca
    10381 aattggtgca cgtaatatgc aaaacttcga gttattaaaa gaagctggcc gtacgaaaaa
    10441 gcctattcta ttaaaacgtg gtttatctgc tacaatcgaa gagtttgttt atgcagctga
    10501 atacattgct tcacaaggta atcaaaacat tattttatgt gaacgtggaa tccgaactta
    10561 tgaaaaggcg acacgtaaca ctttagatat ttcagcagta ccaattttaa aacaaggtac
    10621 acacttacca gtcatggtag atgttacgca tagtacaggt cgtaaagata tcatgttacc
    10681 aactgcgaaa gcagcattag cagtaggtgc tgatggcgtt atggctgagg tgcatccaga
    10741 tccatctgtt gcacttagtg atgcgggtca acaaatggac ttagatgaat tccaagcatt
    10801 ttatgatgaa ttaaagcctt tgtctgattt atataacgct aaaaagttaa aataatattt
    10861 caaggaaact atagactact taactaataa gtcatgttga agtagaatat tatctttgaa
    10921 tcgacaattt taaacttgta gccattctaa gagtatatta cttttagagt ggctattatt
    10981 ttttgtatag aaataaaggt atactgcact taacgattgt tataatactt cgacatttgt
    11041 tcaatttcac aattattaaa gattatgact gatagcagta attaaaatta taactatgaa
    11101 ttatctgtaa aatataatag attcacacat ttgttactga aatgtgaaca tttttcaaca
    11161 aatgcaattg atatttgaaa aggctttctc aaaatattac aattaaaaat gaaaaaagtt
    11221 tatataaaat taaaatatat cgttcgttat catttagcgt ttgtttttat ttcaggcttt
    11281 tcgctaaatt tttccaaaca aaaatatgtt actgtaaatt aaaatatggt aaactatgaa
    11341 aatgaaatga aaacatgtta ttataatgaa taaaacgttt acaaggagga aattatgaca
    11401 gttactatat atgatgtagc aagagaagcg cgtgtctcta tggccacagt atcgcgtgtt
    11461 gttaatggga accaaaatgt taaagcagaa actaaaaata aagttaacga agtcattaag
    11521 cgtttgaatt atcgtccaaa tgctgttgct agaggtttag ctagtaaaaa gacaacaaca
    11581 gtaggtgtga tcattccaga tatatctaat atctattatt cacaacttgc tcgtggactt
    11641 gaagatattg caacaatgta taaatatcac tcaattattt caaattcaga taacgatcct
    11701 gaaaaagaaa aagaaatttt taataactta ttaagtaaac aggttgatgg tattattttc
    11761 cttggtggta caattactga agaaatgaaa gaattgataa atcaatcatc tgtacctgta
    11821 gtagtatcag gaacaaatgg taaggatgca catatagcat ctgttaatat tgattttact
    11881 gaagctgcga aagaaattac gggagaatta attgaaaaag gcgctaaatc atttgcttta
    11941 gtaggtggag agcattctaa aaaagcacaa gaagatgtat tagcaggttt aactgaagtg
    12001 ttaaataaaa atggtcttca attaggtgat acattgaatt gttctggtgc tgaaagttat
    12061 aaagaaggcg taaaagcttt tgctaaaatg aaaggtaatc ttccagatgc tatattatgt
    12121 atcagtgacg aagaagctat tggtatcatg catagtgcaa tggatgctgg tattaaagtt
    12181 ccagaggaat tacaaattat tagtttcaat aatacacgct tagttgagat ggttagacca
    12241 caactttcta gtgttattca gccattatat gatatcggtg cagtagggat gcgcttatta
    12301 acaaaatata tgaacgatga aaagatagaa gaaccaaatg tagttttacc acacagaatt
    12361 gaataccgag gaactacaaa ataaattcac taaattaggc attcatctaa cgactcgact
    12421 tatacgggtg ttggaagaat gccttttatt tatcttttaa aatcgttgca gattaggtga
    12481 cttattgacg agtagattcg taccaactcg ctatatgcaa agctaatttt ttatttttct
    12541 cactaatttc ttttgtgcgg gggacatagg tataatcatt taaacgatct tcccatcttt
    12601 taggtaataa ttcagatgaa taatgtttcc atttattaat ccattctaac ggtaaataac
    12661 cactttgaat tggttgatct attaaactta agaatacatg actccatgca cgtggtacga
    12721 ctctccaaat attgtagcct ccgccaccaa acataattac ctttccattc gtataagtat
    12781 ctgctaaata ttttacaaaa tatggaattt catataatga gtgtaacgta caatttagat
    12841 gagttagtgg atcacgataa tgtatatcga caccatttac gcttagaata atatcaggtt
    12901 taaaactctt tacgacaggc tcaactgtta agttaaaaca ctccaaaaat gatgcatctt
    12961 cggtatacgg ttcaagtggg acatttacag tgtgtccata gccgatatct tcaccgcgct
    13021 cagtatagtg accagagcct gggaaaagaa actttccggt ttcatggata gaataagtag
    13081 taacatgatt atcagcatag aaactccatt gtgtaccatc tccatgatgt gcatcggtat
    13141 ctatgattaa aacgcgttga ttgtattctt tagctaagta ttgtgcggta attgcaatat
    13201 cattgtatat acaaaaacca ctagctcgac caggttgagc gtgatgcaaa ccaccaccca
    13261 agtgacaacc atttaatact ttgcctgaca taataagatc tgctaaagtt aaagcacctc
    13321 caacaattgt ggcactatgg gtgtgcatat gcttaaattg accattctct tcatcattta
    13381 atccatattt cttagcctca tcttcactga taatgccatg tgaagcatgc ttaatagctt
    13441 cgacgtaatc atatttatga attaacatta attcgtcatc tgttgcaatt ctaggttgta
    13501 ctatttgttc tggagacaat aaatttgtat tcaaaagtag ctctgttgtt aattttaaac
    13561 gcatttgatt gaagggatgt tggtcatgaa atcgatattg taataactta tctgaatata
    13621 catatgcagt ttttgataaa tgttgttgca tataatccct ccgatattcc aaaaattaaa
    13681 agaaaaaccg attcatataa cgaatatcat caaacgcttg ttgctgttct aatgtaatat
    13741 ttttgccaat tcttgccatt aaacaattag ctggatgact tgttatttct ggatcatctg
    13801 tagcgaatat ttcaagtcca ccagttgcca ttaaccgctg cattaatttt ttatagtcaa
    13861 atacatctaa ctttgaattt tttaaatccc aatgccagta atattcagta gttataacga
    13921 tataatcctc gaattctggt gtagaaaggc taagttgtat tagcttttct gcaagttgta
    13981 attgcctaaa attgatgctg acttcaattg cccccaattc gattaaatat ggaaggttac
    14041 ctgtagacca gcgctcaatt tcatcaggat agtgaaaagt gacataaccc acaatgagtt
    14101 gatcttggcg agcaacataa attctacctt ctggtaatgt tgtaatttct aacaaagctt
    14161 catactgatc ttcagaatct ctaaatgcgg ttaaatgcac atcgaaagta agcgctttca
    14221 aatcttcgtg tgttaaagga ccttcaataa caaattgctt gtcatgaatg taataatctt
    14281 cggattgata cgtctttaaa tgattcatat tttcaactcc tcaatcgacg tcgtgattgt
    14341 attaaattca ttatatagaa aatttacaat aattaatact agaaaaagga taaaagtaaa
    14401 aattttgaat aattagaaat gttatgtata atattgagaa agaaagcgtt ttcacataac
    14461 aaagggggag tttcaaatga aagtagaagt ttataaagga gcgcaaggca aacataacct
    14521 taaagattat gaagaaacat ataatacttt tgattggaaa gacgtagaac aagcattttc
    14581 ttggagtgaa actggaaaaa tgaacatggc atatgaatgt atagatcgcc atgtagatca
    14641 aggattaggg gataaaatag cgttaaatta caaagatgag cacagaaaag aatcgtatac
    14701 ttataaagat atgcagcggt tatctaataa agcagcgaat gttttgtctg aacatgcaga
    14761 agttgacaaa ggtgacagag tatttatatt tatgtcacgt acacctgaac tatattttgc
    14821 gttgttaggt gttttaaaaa ttggtgcaat tgttgggccg ttatttgaag catttatgga
    14881 aaaggcggtt gcggatagat tagagaacag tgaagctaaa gtgttaatta ctaataaggc
    14941 attgttacct cgagtacctg tagataaatt accgaacttg aaaaaaattg tagtcgtaga
    15001 tgaggatgta gaagacaatt acatagactt cattagtttg atggaaactg ctagcgatga
    15061 atttgacatt gaatggttaa agtcggatga tggtttgatt ttacattata catcaggttc
    15121 tactgggcaa cctaaaggtg tattgcatgt tcaacaagcg atgttagtgc actatatttc
    15181 tggaaaatat gtattagatt tacaagaaga tgatgtttat tggtgtacag cagatccagg
    15241 ttgggttaca ggaacatctt atggtatttt tgcaccatgg ttaaatggcg ctacaaattg
    15301 tatagctggt ggtcgctttt caccagaaca gtggtatagt atgattgaag attttaaagt
    15361 gacgatttgg tatacggcac caacagcttt aagaatgtta atgagtgctg gtgacgatat
    15421 tgttgagaaa tatgacttgt catcgttacg ttcgattcta tcagtaggtg agcctttaaa
    15481 tcctgaagtt ataaaatggg cgaaaaaagt atacggttta acggtgttag atacttggtg
    15541 gatgacagaa acaggtggac atatgattgt taactatcca acgatggacg tcaagcttgg
    15601 ctcaatgggc aaaccattac ctggtattca agctgcaatt atcgatgatg caggtaatga
    15661 attaccacca aatcgaatgg gcaaccttgc tataaaaaaa ggctggccat ctatgatgta
    15721 tcgtatctgg aagaatccag aaaaatataa gtcatatttt attggggact ggtatgtatc
    15781 tggtgattcg gcatataaag atgaagatgg ttacttttgg ttccaaggac gtgttgatga
    15841 tgtaattatg acagctggcg aacgagttgg gccatttgag gttgagtcta aattggttga
    15901 acacgaagca gttgccgaag caggaattat tggtaaacct gatccggttc gcggtgaaat
    15961 aattaaggcg tttgttgcac tgagaaaagg atatgaacca acagacgaat taaaagaaga
    16021 aattcgttta tttgttaaag aaggtttgtc ggcgcatgca gcaccacgtg aaatcgaatt
    16081 taaagataaa ttacctaaaa cacgctcagg taaaattatg agacgtgtat taaaagcttg
    16141 ggaattaaat ttagatgcag gggatttaag tacaatggaa taatgacatg aatgttatag
    16201 aagatttctt tcgaagaata aagggtgaca acatatttca tgttaatgtt taaataatcg
    16261 tttactttac gataagcaat ataaagagct gttaacttgt gtcatatcat ttcgtagaaa
    16321 acatttgaaa atgatgacat aacaataatg gcatatcttt atattgcttt tttattttta
    16381 atatgacctt tggaagatga ctattttaaa taatagaaaa atatagttat caatagtatc
    16441 aagcgctaaa agttgtataa tacaaaactt taataagtga atttattgca aaaatgaaag
    16501 cgctaacccg atttagtcga caagttttta acagttcgtt attatatgaa tgtaagtaaa
    16561 aatttcttag ctacaactta catattataa atgcataaat taaacaaaaa ggggcgaaaa
    16621 aagttgactc atttatcaga tttagatatt gcgaatcaat caacactaca accaattaag
    16681 gatattgctg catcagtagg tatttcagag gatgcattag aaccttatgg tcattacaaa
    16741 gctaaaatcg acattaataa aattacgcca agagaaaaca aagggaaagt tgttttagta
    16801 actgcgatga gcccaacacc agctggtgaa ggtaaatcaa cggttacagt tggtttagct
    16861 gatgcattcc atgagttaaa taaaaacgtt atggttgcat taagagagcc tgctttagga
    16921 ccaacatttg gtattaaagg tggtgcaact ggtggtggtt atgcgcaagt cttacctatg
    16981 gaagatatca acttacattt caacggagat ttccatgcga ttacaactgc taataatgca
    17041 ttgtctgcgt ttatcgataa tcatattcac caaggtaatg aattaggaat cgatcaaaga
    17101 cgtattgaat ggaaacgtgt attagatatg aatgatcgtg cacttagaca tgtaaacgtt
    17161 gggttaggtg gacctacaaa tggtgtacca cgtgaagatg gctttaatat tacagtagcg
    17221 tctgaaatta tggcgatttt atgtttaagt agaagtatta aagacttaaa agataaaatt
    17281 agtcgtatta ctattggtta cactagagat cgcaagccag ttactgttgc agatttaaaa
    17341 gtgcaaggtg cacttgcaat gattttaaaa gatgcaatta aaccaaactt agtacaatca
    17401 attgaaggga caccagcatt agttcatggt ggaccatttg cgaatattgc acacggttgt
    17461 aactcaattt tagcaactga aacagcacgt gatttagctg atatcgttgt aacggaagct
    17521 ggctttggtt cagacttagg cgctgaaaaa ttcatggaca ttaaggcgcg tgaagctgga
    17581 ttcgacccag cagcagttgt agttgtagca acgattcgtg cattaaaaat gcatggtggt
    17641 gtagcaaaag ataatttaaa agaagaaaat gtagaagcag taaaagcagg aattgttaat
    17701 ttagagcgtc atgttaataa tattaaaaaa ttcggtgtag aaccggttgt tgcaattaat
    17761 gcatttatac atgataccga tgcagaagta gaatatgtaa aatcttgggc taaagaaaat
    17821 aacgtacgaa ttgccttaac tgaagtttgg gaaaaaggtg gtaaaggtgg cgttgactta
    17881 gcaaatgaag tattagaagt cattgatcaa cctaattcat ttaaaccttt atatgaatta
    17941 gaattaccat tagagcaaaa gattgaaaag attgtgactg aaatctatgg cggttcaaaa
    18001 gtaacgttta gcagtaaagc gcaaaaacaa ttaaaacaat ttaaagaaaa tggttgggat
    18061 aattacccag tatgtatggc gaaaacacaa tattcattct cagatgatca aacgttgtta
    18121 ggtgcaccat caggatttga aattacaatt cgtgaattag aagcgaaaac aggtgcagga
    18181 tttatcgtag cattgacagg tgcaatcatg actatgcctg gtttacctaa aaaaccagca
    18241 gcattaaaca tggatgttac tgatgatggt catgcaattg ggttattcta atacatcatg
    18301 ttaattgttt aataaagata agtgaatagt ttaatagacc ggactgttgg agatgcatta
    18361 tttcagcagt tcggtttttt gctgtgctaa aaatagattc aatttggcga atctaacggc
    18421 aatgtttgaa gatggttaat taatgtattc caagataaaa agtgggcttg aagaatagga
    18481 aagcgatgca atgaatattc atattaaaaa tttttaataa aataggttgc aatatttaat
    18541 tgggatgcgc tacaattaac actaataatt gatattgata attattatca attaaatacc
    18601 atcttatagg agttgttaac aacatgaaca aacatcaccc aaaattaagg tctttctatt
    18661 ctattagaaa atcaactcta ggcgttgcat cggtcattgt cagtacacta tttttaatta
    18721 cttctcaaca tcaagcgcaa gcagcagaaa atacaaatac ttcagataaa atctcggaaa
    18781 atcaaaataa taatgcaact acaactcagc caccaaagga tacaaatcaa acacaacctg
    18841 ctacgcaacc aacaaacact gcgaaaacct atcctgcagc ggatgaatca cttaaagatg
    18901 caattaaaaa tcctgcagta gaaaataaag aacatgatat aggtccaaga gaacaagtta
    18961 attttcaatt attagataaa aacaatgaaa ctcagtacta tcactttttc agtatcaaag
    19021 atccagcaga tgtatattac actaaaaaga aagctgaggt tgaattagac atcaatactg
    19081 cttcaacatg gaagaagttt gaagtttatg aaaacaatca aaaattgcca gtgagacttg
    19141 tatcgtatag tcctgttcca gaagaccatg cctatattcg attccctgtt tcagatggta
    19201 cgcaagagct gaaaattgtc tcttcaactc aaattgatga tggagctgaa acaaattatg
    19261 attatactaa attagtattt gcaaaaccta tttataacga tccttcactt gtaaaatcag
    19321 atacaaatga tgcagtagca tcgaatgatc aatcaagttc agacgcaagt aatcaaacaa
    19381 acacgaatac atctaatcaa aatacatcaa cgacgaacaa tgctagtgat caaccgcagg
    19441 caacgaccaa tatgagtcaa cctgcaaaac cagtatcttc agccaatgca gatcaagcgt
    19501 caagccaacc agctcatgaa acaaattcta atggtaatac caacgataaa acgaatgcat
    19561 caagtaatca atcgaatggg aaccaacaat atccgccagc ggatgaatca ttacaagatg
    19621 ccattaaaaa cccagctatc attgataaag aacatacagc tgataattgg cgaccaattg
    19681 attttcaaat gaaaaatgat aaaggtgaaa gacagttcta tcattatgct agtactgttg
    19741 aaccagcaac tgttattttt acaaaaacag gaccaataat tgaattaggt ttaaagacag
    19801 cttcaacatg gaagaaattt gaagtttatg aaggtgacaa aaagttacca gtcgaattag
    19861 tatcatatga ttctgataaa gattatgcct atattcgttt tccagtttct aatggggcga
    19921 gagaagttaa aattgtgtca tctattgaat atggtgagaa cttccatgaa gattatgact
    19981 atacattaat ggtctttgca cagccaatta cgaataatcc agatgactat gtagatgaag
    20041 aaacatacaa tttacaaaaa ttattagcac cgtatcataa agctaaaacg ttagaaagac
    20101 aagtttatga attagaaaaa ttacaagata aattgccaga aaaatataaa gcagaatata
    20161 aaaagaaatt agaccaaacg agagttgagt tagctgatca agttaagtct gcagtgacag
    20221 aatttgaaaa tgttacacct acaaatgatc aattaacaga tttacaagaa gcgcattttg
    20281 ttgtttttga aagtgaagaa aatagtgagt cagttatgga cggatttgtt gaacatccat
    20341 tctatacagc aactttaaat ggtcaaaaat atgtagtgat gaaaacaaag gatgacagtt
    20401 actggaaaga tttaattgta gaaggtaaac gtgtcactac tgtttctaaa gatcctaaaa
    20461 ataattctag aacgctgatt ttcccatatg tatctgacaa agcaatttac aatgcaattg
    20521 ttaaagttgt tgtggcaaac attggttatg aaggtcaata tcatgtcaga attgtaaatc
    20581 aggatatcaa gacaaaagat gatgacacat cacaaaataa tacgagtgaa ccgctaaatg
    20641 tacaaacagg acaagaaaat aaggtttctg caacggatac agctgaaaat agcagcactg
    20701 caacaaatcc aaaagatgcg tctgataaag cagatgtgat agaaccagag tctgacgtgg
    20761 ttaaagtgac tgatagtaat attgacaaag atgcgcatca tgatgttgat catttatcag
    20821 atatgtcgga taacactcac ctcgataagt atgatctcaa agaaatggat actcaaattg
    20881 ctaaagacac tgataagggt gtcgataaag atgccgataa tagcgttggt atgtcatcta
    20941 atgtcgatac tgaaaaagac attaataaaa atgaaggcaa agttatacaa ctagcacata
    21001 ataccgataa aaataatcat actggaaaag cagcaaagct tgatggcgtg agacaaaatt
    21061 ataataatat agacaaagtt actgacaaaa aaacaaccga acatccatcg aatgatattc
    21121 ataaaactgt agacaaaaca gtgaaaacaa aagaaaagtc cggcacacca tcgaaagaaa
    21181 acaaacttag tcaatctaaa atgctaccaa aaactggaga aacaacttca agccaaacat
    21241 ggtggggctt atatgcgtta ttaggtatgt tagctttatt cattcctaaa ttcagaaaag
    21301 aacctaaata attaactgga tatagtattt gtatgattaa ctttgtagac aatttgaaag
    21361 caattaagtt ataaactatt gattggttta atggctttcc attagagtat ttaaaataaa
    21421 gaacagcagt gagaaatttt ctaattgaaa ataatcttac tgctgttttt aatatttgga
    21481 tgcattgtta tggtttcttt aaaaagtgag catcaattaa cgcttttttc gatttaataa
    21541 atgtgattta atatcatatt ttaatgcgtc gttgtattct ttttcagtga tttgatcttc
    21601 gattaacata cgctttaata cataatgttg tctttgaata ctatatttca aatctttatc
    21661 cgattttaac gttccatctt tttcgtaggg tgtatagcca tatgggcttt gcaacaaacc
    21721 gataaggtat gcagattgtg caattgataa atcttttggt ggaataccaa ataaactata
    21781 tgaagcggat gcaattccgg aaatattagc gccattataa tctctaccga agggaactat
    21841 atttaaatac gtatatataa tttcatcttt tgagagtagg tgttctaatc taattgctag
    21901 gcgaagttca tttgcttttc tactatatgt tttttcgttg gtaagaactt gatttttaac
    21961 aagttgttgt gtaattgtgc taccacctga actttgatta gtattaaaaa tatcttgtat
    22021 cattgctctt aaaatcgcct ttggtaagat gccatcatgt ttataaaata aagtgtcttc
    22081 agatgacgtt aatgctttaa tgacatttgg acttgatgtt ttaggtccta taatgagtga
    22141 gttttgagaa tggttatact catataacaa atttttgtta ttatgatcta ataattcatc
    22201 gccaggtatt tgtcgaactt tttttattaa agcatcatct gataatgagt cggacgtttt
    22261 agttaaatga tgaaaatata aagacatcgc aatcacagcg atagcaacga tggcaatgaa
    22321 aatcataaat ataaaaacaa tgacatgttt gatagcttta tatatctttt caaaatgata
    22381 tatacgatga ttcaattgat gattgttgtc ttgattcgtc atctttaacc tcctaaatta
    22441 cgtatagtat accatattta agcaatttga cttttatttt aacgttaatt ataattaggg
    22501 aaaatgcata aagaatagga taaaaggaca agtaatatta tttgaaatca tcagagagct
    22561 agtggtcgct gtgaactagt gtttttaata atatgaatac acctttataa ctaacatgtc
    22621 ctacgtttat taatgaacgt atcattaata gagtgaacat tatcttatga ttatgtttaa
    22681 gtttggtggt aacgtgcaat cgcgccctta catatttgtt gtaagggttt ttttacgttc
    22741 aaaaagtttt aatgtatttt aacaagtatt cactaaataa ttataaatag gaggaaataa
    22801 taatgacgaa tgtattaatt gaagatttaa aatggagagg tcttatttat caacaaactg
    22861 atgaacaagg tattgaagat ttattaaata aagaacaagt gacgttatac tgcggtgctg
    22921 atccaacggc agatagctta cacattggtc acttactacc atttttaaca ttaagaagat
    22981 ttcaagaaca tggacatcgt cctatcgttt taattggcgg tggtacaggt atgattggtg
    23041 atccatcagg taaatcagaa gaacgtgtgc tacaaacaga agaacaagta gataaaaata
    23101 tcgaaggtat tagtaagcaa atgcataata tttttgaatt tggaacagac catggtgcag
    23161 tgcttgttaa taatagagac tggttaggac aaatttcatt aattagcttt ttacgtgact
    23221 atggtaaaca cgtcggcgtt aattacatgt taggtaaaga ttcaatccaa agtcgtttag
    23281 aacatggtat ttcatataca gaattcacat acacgatttt acaagctatt gatttcggtc
    23341 atttgaatag agaattgaat tgtaagattc aagtaggtgg atcagatcaa tggggtaata
    23401 tcacaagtgg tattgaatta atgcgtcgta tgtatggtca aacagacgca tacggtttaa
    23461 ctattccgct tgtaactaaa tcagatggta agaaatttgg taaatctgag tcaggtgctg
    23521 tttggttaga tgctgaaaag acgagtcctt atgaatttta tcaattctgg attaatcaat
    23581 cagacgaaga tgtaattaaa ttcttaaaat actttacttt cttaggaaaa gaagaaattg
    23641 atcgcttaga acaatctaaa aatgaagcac cacatttacg tgaagctcaa aaaacattag
    23701 ctgaagaagt aactaaattt attcatggtg aagatgcatt aaatgatgca atccgtattt
    23761 cacaagcatt atttagtggt gatttaaaat cattatcagc gaaagaatta aaagatggat
    23821 ttaaagatgt gcctcaagtg acattatcaa atgacacaac aaatatcgtt gaagtcctta
    23881 ttgaaacagg catttctcct tctaaacgac aagcacgtga agatgttaac aatggtgcga
    23941 tttatattaa tggtgagaga caacaagatg ttaattatgc tttagcacca gaagataaaa
    24001 ttgatggcga atttacgatt attcgtcgcg gtaagaaaaa atacttcatg gttaactatc
    24061 aataaatata attgcatagc taaataaatt agagcctact cattacattc ttaggaatga
    24121 atatgagtag gctcttaatg tacttttctg tctgtaaatt atctaaagaa atctctatcg
    24181 ccttggccag gtgattgacg ttctgatcgg ctttgacgtt ttggttgttc tttttgttgt
    24241 tttaatttca ctttaacttc ttttgtttta ccatcacgga taacggtaac agtgactgat
    24301 tcaccaggtt ttttattttc atataaatag cttcttaaat caacatcatc tttaattttc
    24361 ttgccatcaa tttctgtaat aatatcacct tttttaagat caatatcact atcagctttt
    24421 gcgacataaa taccgtcttc gctatcagta tgaagttgct cgcgctcttc ttcaggtata
    24481 tctttcaaat taattaaacc aataccaatc gaagggcggt ctattttacc atgttttaca
    24541 agttgttcaa ttgttacttt aacttcatta cttggaatag caaacccaat accttcaact
    24601 tgtgtcgcag caattttcat tgagttaata ccaactaaat taccattaat atctactaat
    24661 gcgccacctg agttacctgg gttaatagca gcatctgttt gaagaacgct gacttttgta
    24721 ttaccaccag ttgtctctgc gtcaatagta cgttcacttg ctgaaatgat accagatgtt
    24781 acagagttag caaattgtaa tcctaatggg ttacccattg cgaatacgct atcgccagtt
    24841 tgtacttttg aagagttggc gaattgaatc gctttaatac cttttgtatt ttcaatttta
    24901 agtacagcaa tatcagttac tgcatcttta ccaactaatt tcgctttaac ttgtttttta
    24961 ttatgtaatt ggactctaat ttcatttgcg ccatcaataa catgattgtt tgtaacgata
    25021 taagctgaat tgttgtttat ttgatagata acacctgaac ctactccggc ttcagatggt
    25081 ttagatgatt tgccttttaa taagtcgtct acacttgatg ctttttgcat gttaataact
    25141 ccaacaattg taggggagac agattttatc atttcatgaa cggtaccgaa tttcttgctt
    25201 tgaccgtcta attgattgcc acctttatta tttgttgtct ggacagttga accatcttta
    25261 tttaaaattg tactatttaa tactttgcct ataccaagta ctagaagtgc accaatgatt
    25321 ccagcaatca atgcaacgat gactgtttta aaccatggaa atttaggcct tctgtatcta
    25381 ggtgtttggc tatggtttgt tgtagaatga tctgtatgat taaaatctga catacttaac
    25441 ctccattata tgatttatat atgctttaat tat
//
LOCUS       DAPIDM010000024        25152 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000024 DAPIDM010000000
VERSION     DAPIDM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.024"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            86..1534
                     /locus_tag="RJE42_000896"
     CDS             86..1534
                     /locus_tag="RJE42_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5876340.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSIAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNNVAEYHPPK"
     gene            complement(1614..2951)
                     /locus_tag="RJE42_000897"
     CDS             complement(1614..2951)
                     /locus_tag="RJE42_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDV5876341.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHESLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMNQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            3205..3621
                     /locus_tag="RJE42_000898"
     CDS             3205..3621
                     /locus_tag="RJE42_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876342.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDIISNQSHLKALLLNLDF
                     LIPSDNTPYTLEIICHLLIGSVIYFGFVLLFHTSKRLYYLCYIPLVFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            3744..4634
                     /locus_tag="RJE42_000899"
     CDS             3744..4634
                     /locus_tag="RJE42_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDV5876343.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     ANLYKDLAEHPNVVRIVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(4829..6325)
                     /gene="mqo"
                     /locus_tag="RJE42_000900"
     CDS             complement(4829..6325)
                     /gene="mqo"
                     /locus_tag="RJE42_000900"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV5876344.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            6621..6952
                     /locus_tag="RJE42_000901"
                     /pseudo
     CDS             6621..6952
                     /locus_tag="RJE42_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7012..8610)
                     /locus_tag="RJE42_000902"
     CDS             complement(7012..8610)
                     /locus_tag="RJE42_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDV5876345.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(8804..9247)
                     /locus_tag="RJE42_000903"
     CDS             complement(8804..9247)
                     /locus_tag="RJE42_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV5876346.1"
                     /translation="MNIYDEYRSYIIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLQRNKHLQNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKTINRELSYNIRIELADML"
     gene            complement(9527..9739)
                     /locus_tag="RJE42_000904"
     CDS             complement(9527..9739)
                     /locus_tag="RJE42_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDV5876347.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(10017..11726)
                     /gene="betA"
                     /locus_tag="RJE42_000905"
     CDS             complement(10017..11726)
                     /gene="betA"
                     /locus_tag="RJE42_000905"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV5876348.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTINANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(11987..13477)
                     /gene="betB"
                     /locus_tag="RJE42_000906"
     CDS             complement(11987..13477)
                     /gene="betB"
                     /locus_tag="RJE42_000906"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV5876349.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKEKFEQALIERVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            13546..13641
                     /locus_tag="RJE42_000907"
     CDS             13546..13641
                     /locus_tag="RJE42_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876350.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            13730..14293
                     /locus_tag="RJE42_000908"
     CDS             13730..14293
                     /locus_tag="RJE42_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDV5876351.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAHKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSKN"
     gene            complement(14462..16084)
                     /locus_tag="RJE42_000909"
     CDS             complement(14462..16084)
                     /locus_tag="RJE42_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDV5876352.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            16216..16377
                     /locus_tag="RJE42_000910"
     CDS             16216..16377
                     /locus_tag="RJE42_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876353.1"
                     /translation="MAIIEYLYNFSHNKQNFNSAKTTVLSYDLTNLTNFTRTAFKNIY
                     TLLTNIYLL"
     gene            complement(16393..16503)
                     /locus_tag="RJE42_000911"
     CDS             complement(16393..16503)
                     /locus_tag="RJE42_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876354.1"
                     /translation="MIEYELTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(16602..17138)
                     /gene="nrdG"
                     /locus_tag="RJE42_000912"
     CDS             complement(16602..17138)
                     /gene="nrdG"
                     /locus_tag="RJE42_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDV5876355.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSNARMIEYIVS"
     gene            complement(17135..18985)
                     /gene="nrdD"
                     /locus_tag="RJE42_000913"
     CDS             complement(17135..18985)
                     /gene="nrdD"
                     /locus_tag="RJE42_000913"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV5876356.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCSNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(19232..20602)
                     /locus_tag="RJE42_000914"
     CDS             complement(19232..20602)
                     /locus_tag="RJE42_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDV5876357.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIASN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTLPWIKWVNTALTLAVILAMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(20905..21018)
                     /locus_tag="RJE42_000915"
     CDS             complement(20905..21018)
                     /locus_tag="RJE42_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876358.1"
                     /translation="MGGFVNSLIMKDLSDNDMRYKYEYYNREKIRRTNLIK"
     gene            complement(21091..21699)
                     /locus_tag="RJE42_000916"
     CDS             complement(21091..21699)
                     /locus_tag="RJE42_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDV5876359.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGTVASRRAQTLSQYVEHMTVISPTI
                     TEKLQNMVDKGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVRQ"
     gene            complement(21765..23645)
                     /locus_tag="RJE42_000917"
     CDS             complement(21765..23645)
                     /locus_tag="RJE42_000917"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDV5876360.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQQQRYQRD
                     VY"
     gene            23836..23928
                     /locus_tag="RJE42_000918"
     CDS             23836..23928
                     /locus_tag="RJE42_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876361.1"
                     /translation="MSVINAYSHASLLFFIYLLFIMYQISLSVK"
     gene            complement(24036..24533)
                     /locus_tag="RJE42_000919"
     CDS             complement(24036..24533)
                     /locus_tag="RJE42_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDV5876362.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISANIEILL
                     QESSS"
     gene            complement(24543..24803)
                     /locus_tag="RJE42_000920"
     CDS             complement(24543..24803)
                     /locus_tag="RJE42_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876363.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSYRLSNNIN
                     SNIIYRVFEKCITLYNVYSRCKRALILSEHSKKNIFNIKKAV"
BASE COUNT         7776 a         4758 c         3817 g         8801 t
ORIGIN      
        1 acaaaatatt gattatttgt gctatgatat aacatattaa aaaacaccgt atagaattgt
       61 ttatgaatgt ttgggaggaa agtttatggg aagttttttc aataaaatag cacgaaaaga
      121 agatccggct atctatcaaa ataaagatgg tcatttaaag cgtacacttc gggtgcgtga
      181 tttcttagct ttaggtgtag gaacaattgt atcgacatct atctttacgc tacctggcat
      241 tgttgctgca gaacatgcag gaccggccgt tgcgttatca ttcttactcg ctgctattgt
      301 tgctggttta gttgcattta cttatgcaga aatggctgcc gctatgccat ttgcaggttc
      361 agcctattct tgggtcaatg tattatttgg tgaatttttt ggatgggttg ccggttgggc
      421 tttattagct gaatatttta tcgccgtagc ctttgttgca tcaggattct cagcgaattt
      481 acgcggactt gtgaaaccaa ttggcatcga attacctgca gcattatcaa atccatttgg
      541 tacgaatggc ggttttatcg atattattgc tgctatcgtt attttattaa ctgcattatt
      601 actatcacgt ggtatgtcag aagcagcacg tatggaaaat attttagtta ttttaaaagt
      661 attagctatt attttatttg taatcgtagg tttaacagca ataaatgtta gtaactatgt
      721 gccatttatt ccagaacaca aagtaactgc tacaggtgac tttggtggat ggcaaggcat
      781 atatgctggt gtttcaatga ttttcttagc gtatatcggt ttcgattcta tcgcagcaaa
      841 ctcagcagaa gcacttgacc ctcaaaagac aatgcctaga ggtattcttg gttctttaag
      901 tattgctatc gtattattta ttgctgtagc acttgtgtta gttggtatgt tccattactc
      961 acaatacgca aacaatgctg aacctgttgg ttgggcttta cgtcaaagtg gtcatggtgt
     1021 tgtagcagct attgttcaag ctatctctgt tatcggtatg tttacagcat taattggtat
     1081 gatgttagca ggctcacgtt tactttattc atttggacgt gacggcttat taccttcatg
     1141 gttaagccac ttaaacgaca aacatttacc taatcgtgca ctagttatac ttactattat
     1201 tggtgtttta attggttcta tgttcccatt cgcattttta gcacaattaa tttcagccgg
     1261 tacacttgtt gcatttatgt tcgtttcatt agcaatgtat cgtttgagaa agcgtgaagg
     1321 taaagattta ccaattcctg cgtttaaatt acctttatat cctgtgttac cagcagttac
     1381 atttgtttta gtattgctag tattctgggg attaggattc gaagcgaaat tatacacttt
     1441 aatttggttt attgttggta tcattctata tttaagttat ggacttagac attctaagaa
     1501 aaataacgta gctgaatacc acccaccaaa ataaaaatta aaaaaatcat ttcccatctg
     1561 attaacatcc agatgtggaa atgatttttt attttatatt aagataatcg ctattaccaa
     1621 ccttgtccag atatgtcata ttgatctaag ttacctgctt ccaaagcagt cagtgcatct
     1681 tctatagtgt ttaacgccgt gtctaattgc tcatatgtta tcaccaatgg cggttggaat
     1741 cgcaacacat ttcctgctac agctataata actacgccat gctcaaagca gtaattacaa
     1801 attttaagtg ccgcactggc atcacgtgtt ttgagttttt tgtcggaaac aatatcaata
     1861 ccaatgctca gacctttacc tctaacatcg cctacactat tgtatttaga tacccattga
     1921 ttcattcgtt tcctaacata ttcccctttt tccgcactag cctgaagaag cgattgatct
     1981 tcaatcattt gaattgtggc taatgcagct tcacaactaa ctggattagc acctgttgta
     2041 aataaatgtg ctggtgcttc taaacaattc ataatctctt tgcgtccaac aattgctgac
     2101 atgggcatac cacctgctaa agattttccg aaagtgatta aatcaggcgt aaaattaaaa
     2161 tgcgagactg aactccatgt acctgttcgc ccaaaacctt gttgaatatc atcgacagcg
     2221 attaaaatac catgttcacg acaaatcttt tctaacgctt caaaataccc tggaactggt
     2281 tctaaaagtc caccatcgcc ttgtatcgtt tcaataacaa tacatgctac ttcgtcagca
     2341 ggtacgtatt tcgcaaacat ttcttttagg ggtgctaaat attcttctac tgaattagct
     2401 tgtggctgct cgtacatacc acgatattta tctggaaacg gaatatgata aaaaccattc
     2461 aataacggac cataatgttt gcgcatattt aaactaatag ctgacataga caacgagcca
     2521 aaagttgaac catgatatgc attagtgaaa ctaatgatat aaggacgccc tgtatatgct
     2581 ctggcaaatt taatgatgcc atcatttgcg tctgatccgg ttaatccgaa ggtcactctt
     2641 ttttcaaaat ctccaggtgc aatctcacaa agcttcttag ctaaacgtac caatgattcg
     2701 tgatacatat atgctggtgt ataatgaata aatttatcaa cttgtgcctt tatcgcttct
     2761 gttacttctc taggtgcatg acctacgttt tgagaactcg cactcgataa caaatcgata
     2821 tatgtcttcc cctcaatatc aaccaatgtt gctccatatc catgatcaat aactaacgga
     2881 taatatttaa tacgtcctga tttcgcaaaa taatgttcat cctcttgaat taattgatgt
     2941 gctttactca tatacattcc cctcccattg acagacctta ttcgtataat taccttccct
     3001 acatcgataa ccacattcga tttgatatca cgtgaaaata ctgtcatgtc attatgaata
     3061 ctatccaata tactaataaa attcagattt gtaaatatat tctgataatt aagaatttta
     3121 tatcaatcta ctttatttat gcgcataatt ttgttcgatt agggaattgt ttactatatt
     3181 ccatttgact ctgaggtgat atgaatgcgt ttaaaggtat tgttccattt cattgctgct
     3241 attttcatat cattcatgtt gctatggatg accatgttgt ttgacataat aagcaatcaa
     3301 tcacatttaa aagctttgtt attgaattta gattttctaa ttccttctga caatacacca
     3361 tatactctag aaataatatg tcacttacta attggtagcg tcatttattt tggatttgta
     3421 ttgctgtttc atacttctaa aaggctttac tatctatgtt atatcccttt agttttctta
     3481 tttatagcat tgtatccctt tttagtattt attgcgcaac gtccaatttt tcaatttagt
     3541 gtgacagaat taatcggttg gattattaca catattttct ttatgtcatt aatggcgtta
     3601 gtcataccta gaataaaata atatttttaa ttaaactgac aattattttt cacattttat
     3661 acacttttca aggtttatga cttgagaaat gttctcattt ttatcatact ggaggtgtat
     3721 tgaaaagtta aggagtggaa agtatgaata aagagcaatt agaaaaaatg aaaaatggaa
     3781 aaggctttat tgccgcatta gaccaaagtg gtggtagtac accaaaagca cttaaagaat
     3841 atggtgtaaa cgaagatcaa tatagcaatg aagacgaaat gttccaactt gttcacgata
     3901 tgcgtacacg tgtggtaact tcaccttcat tctcaccaga taaaattttg ggtgctattc
     3961 ttttcgaaca aacaatggat cgcgaagtag aaggcaaata cactgcagat tacttagctg
     4021 ataaaggtgt tgttccgttc ttaaaagtag acaaaggtct tgctgaagag caaaatggtg
     4081 ttcaattaat gaaaccaatc gacaacttag acagcttatt agatcgtgca aacgagcgtc
     4141 acatttttgg tacaaaaatg cgttctaata tcttagaatt aaatgaacaa ggtatcaaag
     4201 acgttgttga acaacaattt gaagtcgcta aacaaattat tgctaaaggg ttagtgccaa
     4261 ttatcgaacc agaagttaat attaacgcaa aagataaagc tgaaattgaa aaagtattaa
     4321 aagctgaact taaaaaaggt ttagacagct taaatgctga tcaattagtt atgttaaaat
     4381 taacaattcc tactgaagct aacttataca aagacttagc tgaacatcca aatgttgttc
     4441 gtatcgttgt attatcaggt ggttacagca gagaaaaagc gaacgaatta cttaaagata
     4501 acgctgaact tatcgcaagc ttctcacgtg cattagctag tgatttaaga gctggacaat
     4561 ctaaagaaga attcgataaa gcattaggtg atgctgtaga atcaatctac gacgcatctg
     4621 taaacaaaaa ctaatttata cattaaacgt attttttgat ataaactact aaaaaggact
     4681 catacatgtg ctttaatggc attgtgtatg agtctttttt gattaaagtt gattttgccg
     4741 tatgtattag attacgtaaa aaaaatcgga cagctgaaaa atctttaaga tttctcagtt
     4801 gcccgatttt aaatatagct attagaattt agttttcgta gtaacctaat tctaagtctt
     4861 ttgaagtttg tttacggatt tttctcatta atttttcgtc ttcaattaat gattcaccgt
     4921 atgatggaat cattttcttg attttaggtg cccattcagt tttgtattca gggaagttac
     4981 gttctaatac ttctaacgca actgaaactg aagttgaagc ccctggtgat tcacctaata
     5041 atgcgattac agtgtggtct tgtgagttaa ccacttctgt accgaattgg atgaatcctt
     5101 taccgtgttc aggtgtatct ttgataactt gtacacgttt accagcagtg tataattgcc
     5161 aatcttcatt acgtgcttct ggatagaaag tacgtaagtg gttcatacaa ccttcttttg
     5221 tcataatcac ttgatcaaat gagtatttaa ttaaaggtaa gtttttaact gctgctgcta
     5281 ataaagttgt aatgttgtat gttttaacag acttgaataa atctaagtta gaaccatttt
     5341 tcaagaattt aggtccaaca ttagcaaatg gtccaaataa caatgttctt tggccgtcaa
     5401 tgtaacgcgt atctaaatga ggtacagtca ttggtggtgt accaggtggc tctttaccat
     5461 aaactttggc atcgtgttgt tcaataactt gtgggtttgt acaagctaag aattgaccac
     5521 tgatagggaa tccacctaaa tgtttacttt cagggatacc tgttttttgt aataatggaa
     5581 ttgctccacc gccagcaccg atgaatacat agtcagttac ttgtttgaat ttctcaccag
     5641 ttaggcgatt tttaacagta acttcccatt gaccatttga taattgttca aaatcaacaa
     5701 cttcatggtt aaattgcact gtagcatttg gatgtgcttc aatgctttta gccattttac
     5761 gtgttaattc accgaagttt acatctgtac cttcgtcaat tttacttgcc gccatgatac
     5821 cagggttatc ttcacggcct ttcatcatca atggaatcca ttttttcatt acttcgatgt
     5881 cttcagtata ttcgatatta tcgaacatag ggaaagcttt catcgcttcg taacgatcct
     5941 ttaagaattt aacattgttt ttacctctaa cataactgat gtgtggtaat ggattgataa
     6001 attctcttgg gttctcgatg ctaccgcttt tcactaagtg accccagaat tgttttgaaa
     6061 tctcaaactc ttcgttaatc actttcgctt tttcgatgtc gatagaacca tcaggttgta
     6121 aaactgtgta gttcaactca cataatgctg catgacccgt accagcatta tttctttcgt
     6181 ttgaactttc gattgcagga cgatccaagc gttcgtaaac gtggatattc cagtctggct
     6241 caatttcttt taacattgaa ccaaatgttg tgctaagtac accggctcca attaaaacga
     6301 tgtctttact attagactta gccattggtt tcacctctcc aaaaattgta aatgtgtatc
     6361 atcaatatga aagttacata aaactgacat atttctttaa aatatcaacg ccatagataa
     6421 cttcctgttt taattgatac gctgtaacaa aatactatag ttagtgctta catgtatatg
     6481 ttaaagcaag cagtggtaaa tgtaaattat aattattcat taactttgca atatattaaa
     6541 tcttttattc atagaagata aatatcaaat caatcataat tatttgacaa caaatagcta
     6601 acgattgttt taatctacat ttggcttata gcattttaaa cctatactct attttgatac
     6661 aatataagtg taaaatcaat cataaaaagg agattcaatg cctatgcatc caagaaaatc
     6721 acttcaatta cctttaatat tgtaaattat tttgaatctt gcacttatag gcattgtgat
     6781 tatcgtttta acacatactg agtttcaact taatgccggt gatagtaatg tttttttatt
     6841 aaactgggat attcgaggtt taacatctta tattttctca ttcttaatac tggttgcgct
     6901 tgtttctaaa ctaattttaa tatttataac tagacgacaa agaacatcat aaataaacaa
     6961 gcagtccagt gcgtatcatc ataccttacg cattggactg ctttgctatg tttattgatt
     7021 gctatctttt tgttgttgcc atttttctat ttcagcatct cgtagttcaa cacgacgaat
     7081 tttacctgag tttgtttttg gtaagtcgtc aacgaattca atctctcttg gatatttata
     7141 tggtgcaact tcatttttaa caaattgttg tagttcttta actagcgtat catcacccgc
     7201 agtatggtcc tgtaaaataa cgaatgcttt aacaatattt cctcgtatat catgaggttt
     7261 tgctactaca gcacattctt taacggctgg atgattcgtt aacgcatctt caacttcaaa
     7321 aggcccaatc gtatagcctg aactaataat aatgtcatct cgacgtcctt caaaccagaa
     7381 ataaccatca tcatctacat gagctaagtc accagtgatg tagtatttac ctgtttgcgc
     7441 tttcgccgta cgttctggct ctttataata tcctttgaaa agtgctggca aatcaagtgg
     7501 tactgcaata ttccctttcg tattagcagg tacgctattc ccctcatcat ctactacagt
     7561 gaccgaacta cccggaatgc ctttacccat tgatccaatc ctttgtggtg tatcttttaa
     7621 aaagcctata agcaaggtac tttcagtctg gccatatcca tctcttacag ttaaattaaa
     7681 gtatttcttg aattgttcaa ctacttctcg atttagtggc tcacctgcag aaacggcact
     7741 atgtaaatgc gttaagtcat aatcatttaa gttctgtaat ttagccatca tacgatattc
     7801 tgtcggtgta caacataaaa cattaatttg atatttttga agcaattcta agtatgtctc
     7861 agggctgaat cttccattaa atacaaaagc agttgcacct gaacctaata cagataagaa
     7921 aggactccat acccattttt gccaacctgg tgctgctgtt gcccaaacta agtcatcttc
     7981 attaatacat aaccaatgtt ttggtgccat ttgtaaatgt gcaaatcccc atccatgaca
     8041 atgtgtaacg gctttaggat tgccagttgt accagatgta tatgacagaa tcgccatatc
     8101 atcacgcgtc gtatctgcca tttctagttt gttacttgcg ttttcttttt cagcttcaag
     8161 tgaaatccat ccatcttttt gaccggcaat aacaaattta gttaacgcat catattcttt
     8221 aattttttca aattcaactg tgaatggctc tagtgcaata acagcattta tttcaccgtg
     8281 tgtgatacgg tattgtaaat ctttagttct tagcatttca gaacatggaa tgattgcaac
     8341 acctaatttt aaagcagcaa tatataattc atatgtcgca atagatcgtg gcatcataat
     8401 gagtacttta tcgcctttag ataaaccgtg cgatgctaaa acattaccta ctttattaga
     8461 ctgttcaatg agctgttggt atgtgactga tatatcttcg ccttcagtat tatgatataa
     8521 aattgccttt ttatctggta tgtggctata tttttcgatt tccgaaataa tgttatattt
     8581 ttcaggcgcg aatagagctg acttttgcat aactaacttc ctttcataca tccacttttc
     8641 ctgtgatgaa cattgtaatt ttataaatga attatatata tcatacgcct atctttacag
     8701 aattttcaat taaatagggt taaataccaa agtcctcgac actacgcttt gacatgacat
     8761 agcattcaag gactttcaaa tgattgaggg ttgatatctc gggctagagc atatcagcta
     8821 attcaatacg aatattgtat gataattcac gattaattgt ttttacatct gcacctttcg
     8881 aagtgccacg atgcttgtgt gtatgcttgt actcagctga attttgccaa tgataaaatg
     8941 cttgcctatt ttcccacagc gtaataatga tatagtgtct accagctgtt ctaggtctta
     9001 aaaaccttaa tgctttaaac ccatcaacgt tttgcaaatg cttattacgt tgcaaaaatt
     9061 tttgctcgaa tgtttcttct tgtccctcgt taatatataa atgatttaac acacataatg
     9121 aatcatttga taaatcattt attgcttcta gaacatcgta atatgcagtg tcattatttt
     9181 tttgtattgt gagacaatca tccagttctt ctattatata acttctatat tcatcataaa
     9241 tgttcataat aaatgccttc atttcattta tatttttggt catattactt tatatctatt
     9301 actaattcat tcccgtattt attaattaca atcatagttt ggcttctttt taaaagataa
     9361 gactttgtaa aaagtattaa tatttcatac aaatgaggac aggagtcgcc cactattttt
     9421 gtgtcttcaa tttcatgatc attatttaac attagtcatg aaaatagccg acaacttcat
     9481 ctgtgaaatc accggccttg tattttagct aactttattt ctgattttac gattttaatt
     9541 gatcatacag agaaagtgat ctttttacaa tttctaaaaa ctcatgatct atattggaca
     9601 tttgatgaaa ataagacaaa atgttttctg ttagcttctc ttgttttggg aatgaatcat
     9661 cttctttaat ccaaatcgct aattcgccta atggtgtttt atcatcttta aatgtttgta
     9721 tatattcgta aaagctcata gtattccttc tctcaattta cttatataaa tcctaccacg
     9781 aaagctttca agaaaacaca attaaatgtc tatttagtga actttttaag gttgttcact
     9841 cttttaatgt ctgccaatta ggtcaattaa tcatcacaat gtacaattaa ctctactttc
     9901 agttcataca ctcacacacc gtttttgaac aacacattaa cttctcattt agataaaacg
     9961 caaaaaagcc tggcaccaat acaatagata ccagactaag agtctactat ataaatttat
    10021 ttagcgtatg gttttacttc gattgcacct tcattttcat catgaacacc atgcttataa
    10081 taatcaatat attgtggctc taaaggcttt ctgccgcgta taatgtctgc tgctttttca
    10141 gctaacatta aaacaggtgc gtgtatattg ccatttgtcg tacgtggcat agctgatgca
    10201 tcaactacac gtaaattttc cataccatga actttcattg ttaacgggtc aacgactgcc
    10261 attggatctg aagcaggacc cattttagca ctacaagatg ggtgtaatgc tgtttcacca
    10321 tctctacgaa cccaatccag aatttcttca tctgtttgca cttctggtcc tggtgaaatt
    10381 tctccaccat tgaatggatc cattgctttt tgagataaga tatttcttgc tacacgaatt
    10441 gcttctaccc attctttttt atcttcttct gttgataaat aattaaagcg gatacttggt
    10501 ttttcgaatg gatctttaga tttgattttc aagctaccac gagagtttga atacattggt
    10561 cctacgtgaa cttgataacc atgtgcgacc gctgcctttt gaccatcata tcttacagct
    10621 attggtaaga aatggaacat taagttagga taatcaactt cgttatttga acgtacaaat
    10681 ccgccacctt caaaatgatt agatgctgct gcacctgtac gtgtgaaaat ccattgtaaa
    10741 ccaataaatg gcatgcgctt gatatctaag cttggctgta atgatacagg ttccttacat
    10801 ttatgttgaa tgtatacctc taagtgatct tcaaagtttt cacccacacc aggtaaatga
    10861 acacgtggct caatgccttt tgattttagg aactctgaat caccgatacc agataattgt
    10921 agtaattgtg gcgtattgaa tgccccacca gacaagatga cttcattagc attgatggta
    10981 tgtagtttgc catttttctt atacgtaaca ccagttgctc ttctaccttc ataatgaatt
    11041 tcagtcacaa aggcacgtgt ttcaacggtt aagtttttac gcttcatagc tggatgtaaa
    11101 tatgctcttg aagctgacat tcggcgacca cgatgtactt gactatcgaa cggtccaaaa
    11161 ccttcttgtc taaatccatt cacatcaggt gttttatgat agcctgcttc aacacctgca
    11221 tcaaagaatg attggaataa tggattggtt gctggtcctc gctttaactt gattggacca
    11281 tcatggcctc taaatttatc ataaggcgct gcaccgtatg ttttttctaa ttttttaaaa
    11341 tacggtaaac agtgcgcaaa atcccaagtt tccatacctt ctggttctgc ccagccttca
    11401 tagtccattg gattaccacg ttgataaatc atgccattaa tcgaacttga tccacctaaa
    11461 actttacctc gggcatgtgc tactttacga ccgcccatat gtggttcttc atctgttgaa
    11521 taaatccaat cgtaaaattt attgcctgaa gggaacatta acgcagcagg catttggata
    11581 aataaatccc aaaaataatc actgcgaccc gcttctaata ctaagacttc tttatcttta
    11641 tcttcactca gacgattacc tagtacagaa cctgcactgc ctcctccaat gatgacataa
    11701 tcatatgatt tatttttgtt actcatcgtt atgacctcca aagtttgttg tatttcaaag
    11761 tgagtattgc atgaaatatt tgttagtggc tgattggtta tcgttgaatt gctaaactta
    11821 gctaaccaaa acactcgatt ttccttttat gattgttact aaaagtcatg aaataatcta
    11881 tgattcattt tgcgtttagt ccaaattatc aatcaacatt tcgccactta tacaaaacaa
    11941 agtgagtgtc ttgtgttctt acaatggcac tcaccttatc taatttttat ttgctaaacc
    12001 aattcactaa ttgtggattt gtatttgtta aaatgtgttt tgaaacaagg tactcttcta
    12061 agccttcttt gcctaattct ctaccgatac ctgattgttt gtatccaccc catggcgctt
    12121 gtgcaaaata tgggtggaaa tcattaatcc acaccgttcc aagtttcaac ttgtttgcaa
    12181 cgcgttgtgc ttttccaata tctttagaaa atacagcacc tgctaaacca tatatagaat
    12241 cattcgctaa ttgaatcgct tcttgttcag tttcaaagcc ttctacagta acgacaggtc
    12301 cgaaaacctc ttcttgtaca atacgcattg acgtatcaca atttgtaatg actgttggct
    12361 cgaagaatag accatctttt aaatcgtctc tatctgggcg tttaccacca acagcaattg
    12421 ttgcgccttc tgctttagct acatccatat aagattcgat cttattacga tgttctgttg
    12481 aaatcactgg tcccatttca gtatcagcat caaaaccatt acctaatttg atttttttca
    12541 cacgctcaat aagtgcttgc tcaaattttt ctttaatact gttttgtact aatattcttg
    12601 atcctgctga acaaacttga cctgcatgga aatatccacc atttaacgct tggtctaccg
    12661 ccaattcaaa atcagcatca tcaaagataa tgtttggatt tttaccgcca agttccaagg
    12721 caatattcgt aacattattg gcagcatttt tcataatatg cttaccagtc tcaatgccac
    12781 ctgtaaatga tacaaggtca acctctttat gacctgacat tacgtcacca acttcagaac
    12841 ctgcacctag aataagatta attgttcctt tagggaaacc aacttcttcc attaattcaa
    12901 aaacacgtat tgttgttaat ggtgtaattt cacttggttt cataactagt gaacaacccg
    12961 tagcaagcgc tggcgcaatt ttccatgatg cttgtaataa cggataattc caaggtgtaa
    13021 tttgtgtaac tacacctact ggttctttaa caattttgct ttctgtatct ggaattggtg
    13081 aatcaatcat ttcgccaccg tctttatctg ctaatccagc aaaatacata aacacattat
    13141 gaatatcatc catatctgca tatgattctt ctaacgtttt tccagtatct aatgtttcta
    13201 atcgtgctaa cgcttcgcga tgttctttga tcttgtccgc gatggcacgt acttttttac
    13261 ctcttgtttc agcagtttct tgcgaccatt caccagactc aaacgcacgt cttgcagcta
    13321 agattgcacg ttctgcatct tcttttgttc cttcagaaac tgtaaatatt acttcttgat
    13381 tataaggatt gataatatct cttgtatttt tattcgcgct ttcaacccac tcaccatcaa
    13441 tatattgacg ctgagataaa tgttttaaaa gttccattca acaacctccg ttaagtttgt
    13501 taaattttta tttaacgaat tttaaaaact tatgttagca tagaaatgaa aaatgtgtca
    13561 atttacaaaa caactcaaga attaagtttt gtaagttcgt tagattttta tttaacgaaa
    13621 catttaactt taatgcttta aatattttta atgtcttttt ttagaaaagg ttttataatg
    13681 tattagtatt tattttaacg tgaccaaaat gatgtacgga ggtgcaatga tgactcgttc
    13741 tcagaacaaa cagcaacaat tagaacaagc aaaagatatt gtaattaatt caattggaca
    13801 gacaatggat ttgtatggca ctaatagaag tgtaggaaac ttatatggca ccatggtgtt
    13861 tgaaggcagt atgacgcttg atgaaatgcg tcatcaattg caaatgagca aaccaagtat
    13921 gagtgctggc gttaagaaat tgcaggaatt tgatattgtt aaacaacaat ttacgagagg
    13981 aagccgcaaa caacatttta tcgccgagaa agactttttc atctttttcc gaaacttttt
    14041 cactaaaaaa ttccagcgtg aaattgacat caatgtcgaa gcagtaaaag atgctcaagc
    14101 tattatcaac ccattacttg aaagcagtga tttaactgaa gctgaaacac aagaggcaca
    14161 caaaattaaa gctcagcttg atcatacaca tgtttattac gaatggttag aacagttaac
    14221 cgaagcgatt gaaagcggtg aaatttttaa atatttccca attccagaac agccttctga
    14281 ttctaaaaat taaaattgac tcaaacaaca acaatgatga taatgcaaga tgaaaacaat
    14341 gcaataggtg cacgaaataa acggtaactt ctatctctac aaatagaaat taccgttttt
    14401 gttttatatg acaatcaatc atggagttat aaaattcaaa atcttatgct catttattga
    14461 attataaatt atcgtcttgt tcttcttctg atacttgaac gattcgcaat gaacgacgtt
    14521 caacttcttt taatttttca gcacgcgttt caagtttaat tctatcgcgt cctaaaatga
    14581 ttaaaaatga tatcatcatg aaaataaaaa taacaattaa tggcacactt gccagtattg
    14641 aagcagtttt caatacttct aatgcacgtt cgccaccaac tagcatcaat gaaaatggca
    14701 ataagcacaa tgcaaatgcc cagaataaac gattggcacg taatggttcg cctaccactt
    14761 ttttctgaga tgctgccgct aaaatatatg aacccgaatc aaatgttgtt gctaagaata
    14821 agaaagcaga tactaagaat agtacaatca tcaatgatgg gaatggtaaa tgatgcacca
    14881 cttcaataat ggttgcctct gtaccatgtg tatttaaata ttgtgttaca ttaaactgtc
    14941 cagaaatttg taaatacaca gcatagttac caaaaatacc aaagaataat acgcatccaa
    15001 gcgttccata aataattgtt cctagcacga cttctttaag gcgtcgacct tttgaaattc
    15061 tagcgataaa taaaccgata aatggcgcat atactaacca ccatgaccag tagaatattg
    15121 tccagtcttg tgggaaattc gtttcttttc gacctttaat accaccgaat ggttctaacc
    15181 atgttgccat atggaaaaaa tctctcaaca tatttccgaa ccctgtcact gtcgtttcca
    15241 taataaaaac agtcggtcca ataataaata taaaggctaa aagtacaaat gatagccaaa
    15301 cgttgatatc acttaacttt tgaatacctt ttttcaatcc tgtatatgaa ctgatggcaa
    15361 atataacagt gattgttaat aaaatggccg aacgtaaaat catattttta ccatctaaac
    15421 cagttaatct ttctatgcct gcagaaatta atggtacacc taacgctagt gatgttgccg
    15481 caccacctag caatccaaag ataaataaga tatctacaac tttacctaca aatttatctg
    15541 tttgaccttt taaaatcgga cgacaagctt gactaatttt atacaccggt tgttttttaa
    15601 caaatactaa ataaccaatt ggtaatgctg gtaaaacata aatagcccaa gcaattggtc
    15661 cccagtggaa cataccatac tgcgtcgcat attggagtgc ttcatcactc atacttttcg
    15721 cgccatttgg tggaacttga tagtaaaaag cccattcaat aacgccccag tataaaatat
    15781 cagagcctat gcctgcacaa aacagcattg ccgcccatgt aaatgtatta aattctggtt
    15841 tatcacttgc tttaccaagt gtgacattac catatttacc aaatgcgata tacattacaa
    15901 agcaaaaaat cgccagcccc ataaataaat atatcgaacc aattgaatca gaaatggcac
    15961 tattaatacc agtgatgata tcttcacttg cttttggaaa agccatcata ggtataactg
    16021 caaaaagaag tacagctact gtccctataa aggtcgtcca gtccataact ttctcttttt
    16081 tcaattgtgc tccccctaat tattaatttt atgaatcctg tttcgattta tctcaaaatg
    16141 taataattat attgattcac aaaattgaca ataactaata tttaataata atgcaaattt
    16201 catacaattt gaaacttggc aattattgaa tatttatata atttttctca taataaacaa
    16261 aactttaata gcgctaaaac aactgtgtta agttatgatt taacgaattt aacaaatttt
    16321 actagaacgg catttaagaa tatttataca ttattaacga atatttattt attgtaaaac
    16381 gctaccaaaa agttagactt ccttcccact aaaataccac ttttctcttt caactttttt
    16441 aaaaaacgga tatgcaactt ttaatattgg tatcaaaatg attgttagtt catattctat
    16501 caatatattt ttctaaagaa ttgcttttat taactttcaa ttatgtacct aacctaaaaa
    16561 aagccaaggc aacggatgtt accttgactt ctaatacata ttcaactaac aatatattca
    16621 atcatacgcg cattcgagag tgattgttgt acatctataa tgcgttgatt taaagaacct
    16681 ttatatggta aatcaggttt gaataagtgt tgtataaata gcccatctac taaaacgtca
    16741 atgtatgata ataactctcg acgttctgta caatcatttg ctaaatattc atataaaaat
    16801 ccagtccata cccaaattgt ctttgtattt ccaaaacgtg ctcgaaatgc tttaacaaga
    16861 tttaatgtaa tatccaaatt acaaaatggt tcgccaccta gtagacttag cccagatata
    16921 taatcatgat cgcaatcatc taatatttct gctaatattt catcagtgta tttctcgcca
    16981 tatctgaact tttgtgaggc tttgttgtaa catccaacac aattaaatgg acatcctgat
    17041 acataaacac tgcatcttac tccttcaccg tcaacaaagc tatttgattc tattttagca
    17101 atataacctt gtccttgttt aatgtctaaa agtgtcattc tttaggcgct ttcatatgtt
    17161 ttactcgtgc gcaaatttct ttatgacggc ctttaattac tggacgttga actggatttc
    17221 ctaagtaacc acatgttcgt ttaacgacat caactgtttt aggattatca ttgctacagt
    17281 tcgggcattt aaatcctttt tcagttgctt caaaatctcc atcgtaatca cattcataac
    17341 aatgatcaat cggaatattt gtacctaagt aaccaacttt gtcataagag tagtcccata
    17401 ccgcttctag tgctttcaaa ttgtgttgca gtttcggata ctcacaatag tgaatgaaac
    17461 caccactcgc ataataagga taatcttttt caaaatctaa cttttcaaaa ggtgtaacat
    17521 ctttacgtac atcataatgg aaagagtttt ggtaatatcc tttatctgta atgtctttaa
    17581 tatctccaaa tctctcttga tctaaacgac aaaaacgatc cgttagcgac tcactcggcg
    17641 tactataaat actgaaccaa atgtcatata attctgtcca tttcgtttga taacgtttca
    17701 tttctttaag aatttcaagc gtaaatgctt ttgcttcttg agatgtttcc cagtctggac
    17761 catagaaaac agtagctgtt tcgtataacc caatatagcc cattgaaatc gttgcacgtt
    17821 tatttttaaa taactcagca acatcatctg tttcttttaa tttatagtta aaagcgccac
    17881 ttttatataa aattggtgcg ttattcggta cagcatcttt caaacgattt atacgataaa
    17941 gtaatgcatc atgtaacaca tcgatacgtt cataaaagat ttcccagaat ttcgtcatat
    18001 taccggcaga ttctaatgcc attctaggta aattaagtgt aacaacacca agattacaac
    18061 gaccattatt ttcaaaatga ccttccgcat ctttccaact tggtaaaaat gagcgacaac
    18121 ccattggcgc tttgaaatca cctaatattt ctacgagttt gtcataattt aaaatatctg
    18181 gatacatacg tttcgttgaa cactttaatg ctaattgttt aatgtcatag ttcggatctt
    18241 gcggactaaa gttggttcct tttttaattg aaaatacaag ttttgggaaa atcgctgtcg
    18301 tgcggtcttt tcctaagcct ttgatacgag tatttaagat agcttgttga attttgcgac
    18361 ttaaatgatc tgtacctaag ccgaatccta atgttacaaa aggtgtctgt ccattagatg
    18421 tatataaggt attaatttca tattctagac tttcaatcgc atcattgatg tctttagtga
    18481 cttgttgatc aacataacga tcaatttcag attctttgac aaattgcttt gcgatattcc
    18541 tatgttgttc ttcattatgt cgtgcatatg tactaagtaa ttcgtcaacg cggtcaaccg
    18601 tacagccacc atattgactg ctagaaacat tggctataat ttgtacaagc tgcgctgatg
    18661 cagtttgtat tgattttggt gaagttacat tcgcgttgcc tatttcaaat ccattatgta
    18721 gcatattttt agcatctatt aaacaacagt ttgttaacgg ttggaatgga tgataatcta
    18781 agtcgtgaaa atgtatgtcc cctctttgat gtgcatctgc aacgtgcttt ggtaataaat
    18841 gatttaatgc gtaagattta gaaacgatac ctgctgttaa atctctcatt gttgaaaatg
    18901 tatcactatc tttgttagca ttttcgttaa caatagcagg atctttagaa atcaaacccg
    18961 ttaatgcttc ttcgatttga ttcattttac cccttcttcc tatatattgt gtctactcat
    19021 tacgttatac cactatatat agtattttca aatttttatt agtctgttaa ctaaaacgtc
    19081 acaaagttat taacatttag tgaaattcat attttcacac aattatttca ttaattttta
    19141 aaattgttta aagattcgtc cgtcatttcg acatgtttat gcaactatta tttcattttg
    19201 taacgggacc atagtttcta ttttttcata cttaaatcgt cacaattccc atcaacattg
    19261 caattactaa cataacgatg gcgaatcccc aaatccaaaa gaatgcatac ttaatatacg
    19321 tgcccatgtt tgcctctgct aaaccaattg ccaaccataa ggctggtgaa aacggactga
    19381 caaatgtacc tataatattc cctatgacca ttgaataagc agttgataca gatggtacac
    19441 caaattgccc tgctgtttgt tcgacgatcg gtaacacagc aaaataataa gcgtctgtgc
    19501 tagttaataa atctaatggt acgccaagta aacctacaat aatatgcaag tatggtccta
    19561 cttctgcagg aatcactttg attaaatttg tcgcaatcgc tttaagcata ccggtttcat
    19621 ttagtacacc taaaaacata cctgctgcaa taatcactgc agccatcatt aatgcattcg
    19681 gcgcatgcgc tcttaatcgc tccatttgtt catccactga tttaaaatta ataacaagtg
    19741 ccaacgaaac acctatcatg aatgcaaatt caggtggcgc aatatttatt aacatcgcta
    19801 gaataacagc taaagttaaa gctgtattca cccattttat ccatggtagt gttcgtgcac
    19861 gtccttttac aggaaacctt acatcttgat ctcgttcata tacttcaact aatttatgta
    19921 catctatatc ttgtgtttgc ggtaattcat tgctagcaat tgcttttttg atacgttttt
    19981 gttctttaaa tccaagatat accgcaaata acataacaag aatgaaacct attatttgaa
    20041 taggtattaa tccataccat aattcattga cacttttggc ttttaacact gcagctacac
    20101 gtgccattgg acctccccaa ggtaccatgt tcataatcgc cgcgcttaat gctaatagta
    20161 gaatcaataa atatttattc atatttaacg ctttatataa aggtaataat gcaggaatag
    20221 aaagcaaaaa tgttaccgca ccggcaccat ctaattgggc tattgtgcca attaaagctg
    20281 tcattgcaca gacaataacg acattgcctc gtgtcattaa tattaagcgt ttgacaagcg
    20341 gcttgaataa accactatcg ttcatgatgc caaagaaaat aatggcaaag ataaacataa
    20401 taacaacgtt gatgacttga tctaaccctt tagcaaaaaa tccaaccaaa tctgtcacac
    20461 tatatcctaa aatcattgcc cctaagcaag gtatgattgt catacctaca actggattta
    20521 tctttttggc aatgagtaaa cctacaattg aaataataat aatgagcccc attactgtta
    20581 accacatatt atcactattc atattatttt ctccctccac ttttaataca atttacataa
    20641 tccccctctt taacttaaat ataataatta atataatttt ttatatttaa gttaactcat
    20701 tttgtcaaaa atttcttttt tgtgtaacta tcaatagtga agtttgattt tcctacttga
    20761 taacatggca tgactaatcc ttgtatatcc tgtcctgtat ttcattttga tttatcattt
    20821 ttattattgc tttctattgc acaaaaaatc tggctaaagg tcgctatgat agtaacgact
    20881 tcaaccagat ttcaatttat ccatctattt tattaagttt gttctacgta ttttttctct
    20941 attgtaatat tcatatttat aacgcatatc attatcgctt aaatccttca ttattagact
    21001 attcacaaag ccacccatgt cttaatttca atgattaagc gttaaagtta aaataagaag
    21061 acttacctta gacggttaga cgacccgcta ttattgtctt acatccaacc acgctaaaaa
    21121 ttgagcttgt ttgtcatgat ttaaatattc ttgtgacaca atttgtgaca gtaactgttg
    21181 cttttcgtta tatgttatat ctaataactt tattttctgt cggcaagtat ataaaaagtc
    21241 gatatacgaa ctgtatgatg gtggatataa cgcctcaagc tctgccataa tcgattttgt
    21301 caacttagga cttgcgccat ctgtagatac actgatagtt agcttgtcgc ggtgtagtgc
    21361 acttggaaag acaacattgc catttgatgc atctccaaca ttattaaaaa gggcatgctc
    21421 aggtaaggct tgttttaccg cttcattgac acgtggctca ttggttgctg caataactag
    21481 atacgcgtct acaatatcac ttggttcaaa ttctttttcc ttccatatga cgacaccttt
    21541 atctaccata ttttgaagtt tttcagtgat tgtcggactg atgaccgtca tatgttcaac
    21601 gtattgactt aatgtttgtg cccgacgact tgcaactgta cccccaccta ctataaccac
    21661 gttcttattt gttaaatcta tcatcaatgg catattcata tcgcgctaca tcctttatat
    21721 ctttttacgt catttcacct taaaacattt aacacattga agcattaata aacatcgcgt
    21781 tgataacgtt gttgctgttt catttgacgc aatatcgtct cagcttcttc ttgagaaata
    21841 tgacgttctt ttaccaatac atctttaatg gcttgatgga catctttcgc catacatttt
    21901 tcatcgccac aaatatagat tgctgcgccc tgctcaatcc attcattgaa acgttcactt
    21961 tcttccatta tacgatgctg tacatattct ttgtgttctt ggtctcttga aaatgctaaa
    22021 tctacgcgtg ttaagtttcc attttcaagc cattcttcta tttcttcttc atataaaaag
    22081 tcagaactac ggtgttgatc accaaagaac aaccatgttt ttccagtcat accaagttct
    22141 tcacgttctt gtaaatatgc tctaaaagga gcaataccag tacctggacc aatcataatt
    22201 accggtatat cttgcttcat cggaaatttg aagttcggat ttttcttcaa ataaattggt
    22261 actatatcgc ctggtttaat acgctcagca aaatgaactg agcatacacc ttttctctca
    22321 cgtccatgtg cttgataacg aaccgtacca acggtaatat gcacttcatc tggcgttgcc
    22381 ataaaactac tagaaatcga atactctctt ggtggtaatt ttctaaggat ttgatacata
    22441 ttctcaggtt gtaattctat agttggaaaa tctgttatta aatctataaa gtcccgattt
    22501 ataacatatt cacgcgccca tgactcatct tgaatacgtt cagataattc ttcattgtca
    22561 aaatagatat ctgcattttt caataatggc aatgttaatt tagtaaattc aaaatgtgat
    22621 gttagtgctt caacaatagg aactgtatca ccatgatcat taattggcac cggagattgc
    22681 ggatcccaac ctaacatgga tattagtttt tcaaccaatt cagggtcgtt ttgcggtaat
    22741 gctactatac aatctcctgg ttcatatgat tcactaaaat catcaagtaa aaattctata
    22801 tgtcgtgttt ctttatttga atcggtacca tttaaattga tattcgctaa aacttctgct
    22861 tggtatggat ttgatttaga atatttcttt tctttggcag acttaattga ttcacttatc
    22921 accgattcac tttgaatacc ttctgatgtg gtatcaataa tattaatgat gtctgccatc
    22981 cacttttctg cgtcttcttc ataatcaata tcacaatcta cacgcttaca tatacgctca
    23041 gcgcctagat tttctagtaa agcatctaca tctttaccgg cttgacagaa aaattcatat
    23101 gtttgatcac ctaaagctag tactgaatat ctcacatgat ttaaattagg tgcgttatcg
    23161 tcttctaaaa attcaaagaa atcccatgca ttatcaggcg gttctccttc accatgagta
    23221 gacgtaataa taaataaatc ttctaactgt gcgatgtttg tcgtatcata ttcatccatt
    23281 gacatcaaaa caacttgatg tccgatatca cttaaacgtt ctgaaaaaat ttcagctaaa
    23341 cgcatggcat tacctgattc agaaccatat agcactgtaa catgccgctg attagcttcg
    23401 atatgtggct ctttattttg taacatatac gttgttgaag ttgcttgttc ttctgatatt
    23461 tgtgattgtt gccacacttc tgtctctaat tgttgttgat tactatcact tgtcgtatcg
    23521 ttactattag ctagtaaaaa accactaagc cactgttgtt ggctctctgt taatgtttgc
    23581 agcagattgt taatctctgt gacttgtttt tctgtaaatg gactattaga tgtatttaat
    23641 ttcaaatatt ttcacctcaa taaacttgaa atagatgatt gactcatttt ttatcgatag
    23701 ttcagtcaat tttttaatat gtatggtata aattaaaaca aacttgagtt gtgtctgata
    23761 gttatactta caaatattta caaccttcat gttgcttgaa tgacacagta attcaaacaa
    23821 aacgttgatt atgtcgtgtc agttattaat gcatacagtc atgcatcgtt gttatttttt
    23881 atttatttac tatttattat gtaccaaatc agcctttcag taaaataggt caaattactg
    23941 attttctaaa tataaaatgc ctcctaatga catactatta gtacatcatt aagaggctct
    24001 tgtgttattt gcatactaag cgctcaattt taaatttaag atgaagattc ttgcaataat
    24061 atttctatat ttgccgatat atccaatgga tcttccattg gcaagaatcg atttacaaca
    24121 ttgccttgtc gatcgattat aaattttgtg aaattccatt ttattgggga cccaaagatt
    24181 cctggttgtt cattctttaa atgcgtaaat aacggatgtt catcgttccc gttcacagat
    24241 attttagcta gcactggaaa tgtaacacca aatttctcac gactaatttt caagatttct
    24301 tcattagaac ctggttgtcg attgtcaaaa ttattattcg gaaaactcaa cactacaaac
    24361 ccacgatcct tatatttttg aaacaaagtc tctagttttt tcaattgttc gctatatata
    24421 cattctgttg cagtattaac aactaaaatc actttacctc taaatgcttc taatttataa
    24481 gttaagcctt tataatcact tacttcgata tcatacacat ttctattatt cataagacac
    24541 ccctacacag ccttttttat attgaatatg ttctttttag aatgttctga taaaataagt
    24601 gcgcgtttac accgcgaata cacattatat agcgtgatac atttttcaaa cacacgataa
    24661 ataatgttcg agtttatgtt gttgctcaac ctatacgatt taccgtcttt tttcacctgt
    24721 cttactttat taaaataatc acattgtata gctgtattag tccagctaca atatctttcg
    24781 ttataacccc tcattataat catccttatt ttctattttt aaaaagacaa ttagaccgct
    24841 ctttaaacta tagattaata cttaagttaa actcatacat actgatacca tacgttaaat
    24901 ctaacaattt aaaattcgtt ataactatgg attaaagagc tgcccaactc atttaatcct
    24961 taaaaacttc acatgtgatt gtttattaag ccctccttta tcttattaaa tatccttata
    25021 acccttttaa aattaaactg acacactcat acattgttac acgcgacgat aaacgtcata
    25081 ttattgcata tcattaaaac aagtcacttt gtatcgtgtg cgaccgaata catattaaat
    25141 catcaataat ga
//
LOCUS       DAPIDM010000025        25083 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000025 DAPIDM010000000
VERSION     DAPIDM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.025"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            79..252
                     /locus_tag="RJE42_000921"
     CDS             79..252
                     /locus_tag="RJE42_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDV5876364.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     SDQSDLMRAEVTE"
     gene            253..654
                     /locus_tag="RJE42_000922"
     CDS             253..654
                     /locus_tag="RJE42_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876365.1"
                     /translation="MYSKESIVNMIGTHKMKCNVLADVIPEYDSNSIAQYGIQATLPK
                     PQGENSSKVEDVVVRLERANKRYAQMLKEVEFINQSQQRLGHVDFCFLELLKKGYNRD
                     AIIKKMPNSKLNRNNFLARRDELAEKIYLLQ"
     gene            1002..1382
                     /locus_tag="RJE42_000923"
     CDS             1002..1382
                     /locus_tag="RJE42_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV5876366.1"
                     /translation="MSNMQNNATFGAYLELTKKQQAYIQIKTDTGAKDMDIAEQIDVN
                     RATISRWKTNDKFREGFKGYQAEHLQKQVPKALQTMINLLDAKSELVRFQASKDILDR
                     TGYNPVDKQEITMQSAVTFNDDID"
     gene            1366..2631
                     /locus_tag="RJE42_000924"
     CDS             1366..2631
                     /locus_tag="RJE42_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDV5876367.1"
                     /translation="MTISINLSDLLPKHFHPLWKVTKDKEVLNVVAKGGRGSGKSSDI
                     SIIITQLIMRYPMNAVVIRKTDNTLATSVFEQIKWAIEEQKVSHLFKVKVSPMEITYI
                     PRGNRIIFRGAQNPERLKSLKDSRFPFSIAWIEELAEFKTEDEVTTITNSLLRGELDE
                     GLFYKFFFSYNPPKRKQSWVNKKYESSFQADNTFVHHSTYLNNPFISKQFIQEAESAK
                     KRNEQRYRWEYMGEAIGSGVVPFNNLRIEEIPQRQYDTFDNIRNAVDFGYATDPLAFV
                     RWHYDKKKRVIYAMDECYGVQISNREFANWLKKKGYQSDEVFADSAEPKSIAELKQEH
                     GIKKIKGVKKGADSVEFGEQWLDDLDAIVIDPRRTPNIAREFENIDYETDKDGNVKPK
                     LEDKDNHTIDATRYALERDMRQNKLSILR"
     gene            2650..4056
                     /locus_tag="RJE42_000925"
     CDS             2650..4056
                     /locus_tag="RJE42_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV5876368.1"
                     /translation="MPWDKPYGEEVVEQLKPQFETQEEMIVRLIDDHRKQLDKITVGQ
                     RYYDKDNDIVKQMKKVDVHGNIDYDKPDWRITTNFHQNLVDQKVSYVASKPVTYSCED
                     ENVLKVIHDVLDTRWDNKLIDILTATSNKGIDWLQVYINENGEMKLFRVPAEQAIPIW
                     IDKEREELKSFIRYYKFNNEEKVEFWTDATVTYYVLENGGLIPDYYYGANHVQSHFSN
                     GNWGRVPFIAFKNNPEEVSDIWMYKSIIDAIDKRLSDAQNMFDESVELIYILKGYEGE
                     DLEEFMRGLKYYKAINVDGDGGVETIQVEVPVSSTKEYIDLMRAYIMEFGQGVDFQTD
                     KFGSAPSGIALKFLYGNLDLKANKLKNKATVAIQELIGFIIDFNNLKMDVKDIEISFN
                     FNRMMNDAEQSQIIAQSQYLSRETLVKSSPLVDDYKAELERIEQEQMEYNKQLPNLDD
                     GGADGAQQQERSNDKQSE"
     gene            3995..4975
                     /locus_tag="RJE42_000926"
     CDS             3995..4975
                     /locus_tag="RJE42_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDV5876369.1"
                     /translation="MTEVLTVPNNKKDLTINNQNDIDEYIDSLISKAEKPIEQLFANR
                     LKEIKQIIADMFEKYQSDDMYVTWTEFNKYNRLNKELTRIGTMLTDDYRQIAKMIQKS
                     QEDAYIEKFLMSLYLYETASQTSMQFDVPSKEVITSAIEQPIEFIRLVPTLQKHRDEV
                     LKKIRLHITQGIMSGEGYSKIAKAIRDDIGMSKAQSLRVARTEAGRAMSQAGLDSALV
                     AQKNGLQMYKYWQATKDTRTRDTHRHLDGAKKKIDEPFKSSGCVGQAPKLFVGVNSAK
                     ENINCRCKLMYYIDEDDLPSTTRVRKDDGTTEVTPQMTYREWEKYKRKRK"
     gene            5073..5669
                     /locus_tag="RJE42_000927"
     CDS             5073..5669
                     /locus_tag="RJE42_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDV5876370.1"
                     /translation="MDLYTLLGQFKDGEIDKQKVIDAIDESKSGMVPRSRLNDKNTEI
                     EELKEEISKRDEQIVKLQDSVKDDSEIQKELEELKNQNSEWETKYKETQLNNAVKLAV
                     AKEANDANDILAFINKDELELVDDGTVKGLDEAIKTLKESKPYLFASSKPVGKTPQGG
                     GNPDSGVTKEKFDNMSVAERNELYLNDRETFEKLVNQN"
     gene            5690..6514
                     /locus_tag="RJE42_000928"
     CDS             5690..6514
                     /locus_tag="RJE42_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HDV5876371.1"
                     /translation="MAQGLTKTSNQIIPEVLAPMMQAQLEKKLRFASFAEVDNTLQGQ
                     PGDTLTFPAFVYSGDAQVVAEGEKIPTDILETKKREAKIRKIAKGTSITDEALLSGYG
                     DPQGEQVRQHGLAHANKVDNDVLEALMGAKLTVNADITKLNGLQSAIDKFNDEDLEPM
                     VLFVNPLDAGKLRGDASTNFTRATELGDDIIVKGAFGEALGAIIVRSNKLEAGTAILA
                     KKGAVKLILKRDFFLEVARDASTKTTALYSDKHYVAYLYDESKAVKITKGSGSLEM"
     gene            6531..6857
                     /locus_tag="RJE42_000929"
     CDS             6531..6857
                     /locus_tag="RJE42_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HDV5876372.1"
                     /translation="MYKVIERFEDAQDNGREYQVGDIYPRDGLEVSEERFTELSTTNN
                     RRNLIAIKLVEEKQTEQSEANAEEQKSLSDMKVAELKELAKKREIKGYSDMKKDELIK
                     VLEGVK"
     gene            6857..7171
                     /locus_tag="RJE42_000930"
     CDS             6857..7171
                     /locus_tag="RJE42_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDV5876373.1"
                     /translation="MDTKDVKMINGLSLNDSSNDEQIEYLIEEYKSVAEDYCNQKFDD
                     KEVPSGVKKFIAECIKFGTTGNISARTMGTVSYTYVTDIPSSAYAYLMPYRKLSWGKR
                     YV"
     gene            7164..7499
                     /locus_tag="RJE42_000931"
     CDS             7164..7499
                     /locus_tag="RJE42_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDV5876374.1"
                     /translation="MFNPFDEFPHTIEIGEVEVVGTYPKEYERFKSNETIKGFMDTPT
                     SSETLKFHQMSKDFDRNLYTPYHIPITNKTLFNYEGKTYEVVGEPVDQGGQHEINLTR
                     LRVRSIGKG"
     gene            7486..7899
                     /locus_tag="RJE42_000932"
     CDS             7486..7899
                     /locus_tag="RJE42_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDV5876375.1"
                     /translation="MAKVKYGNWDLVKELENYERDIERWVKRGIAKTTTKIHNTIISL
                     MPVDTGYLRESVTMDFKDGGLTGVINIGSEYAIYVNYGTGIYATGAGGSRAKKIPWSY
                     KDANGKWHTTKGQHAQPFWEPAIDAGRAFFNKYFS"
     gene            7912..8349
                     /locus_tag="RJE42_000933"
     CDS             7912..8349
                     /locus_tag="RJE42_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDV5876376.1"
                     /translation="MWVSVERYLFNKVYNKLKSNPIIQKQLGGRVFDCVQKDAVYPYI
                     VVGETNVTNKETTTSMVEDVGITLHVYSQARNRDEASQTIQFLGFVLNNEIEIDYYSF
                     IKSRIDTQEVITDIDQYTKHGIIRLVFKYRHNTLQRSVTNGAG"
     gene            8336..8896
                     /locus_tag="RJE42_000934"
     CDS             8336..8896
                     /locus_tag="RJE42_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV5876377.1"
                     /translation="MAQDKYIVALQIADKDLAKKLTIEEATLLGSLAEGGHTISNDLA
                     EIIQGGKKDYSRNSVEEEIKLTLDVVPGDKGQLALKESVKQFKQLRVWIWETKKRDGK
                     HHGVFAYVVIEEHEWSFDDEDNKIEITAKVKFNSADGTINDLPKEWLNPSALAPVVEF
                     EDMNAYEDSYENRTKKTTAGSSDLSM"
     gene            8958..9452
                     /locus_tag="RJE42_000935"
     CDS             8958..9452
                     /locus_tag="RJE42_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876378.1"
                     /translation="MTETTFNPITSLTINNEEVKAKATFMFDKTAKKFATEQEDNKGR
                     KQTISGFTNVYNALLERDTVAIVDFWECATAYLGKSAPKREDIEAEIMEIIERENDTL
                     NLLQGALDVMNNSGFFKQKSRLFWTQMNQAPSLAKEDEKEGAKAGIEMMKNNYKEIMT
                     VAPY"
     gene            9473..9814
                     /locus_tag="RJE42_000936"
     CDS             9473..9814
                     /locus_tag="RJE42_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876379.1"
                     /translation="MTSRYIGYMSNDELMSMLPAEWNDWIIGARQALIDQRDIALYGA
                     QYNAVAQAGKSLKRFVRQNEREHYIIRGQEGEYERMKQRELAKNKRKREIQKQGTRKF
                     LNSLKTSHKGG"
     gene            9817..12786
                     /locus_tag="RJE42_000937"
     CDS             9817..12786
                     /locus_tag="RJE42_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV5876380.1"
                     /translation="MEKNFLARVTAIISDFKRNIRTAQRMAKTDIPDEIKTEVTANIR
                     DYQRELTRAKSMAQRWREHKVNIDADASKVKQVISFVKAELSNIRRKKVEIDGDASGL
                     KRNVTTSKAMLAGWRKHTVKLDFDTTGMTKMQVALTAGKRALEQYQSTMDGIASNIRT
                     FGTIFAQQVKGLMIASIQALIPIIAGLVPAIMAVLNAVGVLGGGVIGLAGAFSVASVG
                     AVGFGAMAITALKMVKDGTLAVTKEVQNFRDASDQLKTTWQGIVKENQASIFNAMSAG
                     IRGVTSAMSQLKPFLSEVSMLVEANAREFENWVKHSETAKKAFEALNSIGGAIFGDLL
                     NAAGRFGDGLVNIFTQLMPLFKFVSQGLQNMSIAFQNWANSVAGQNAIKAFIDYTTTN
                     LPKIGQIFGNVFVGIGNLMIAFAQNSSNIFDWLVKLTSQFRAWSEQVGQSQGFKDFIS
                     YVQENGPTIMQLIGNIVKALVAFGTAMAPIASKLLDFITNLAGFIAKLFETNPAIAQV
                     AGVMGILGGVFWALMAPIVAISSVLTNVFGLSLFGVIKQILSFVRTSSLVTGAMQLLM
                     GVFGSISAPILAVIAVIGAFIGVLVYLWKTNENFRKTITEAWNGVKTAVSGAIQGVVG
                     WLTELWGKIQSTLQPIMPILQVLGQIFMQVLGVLVIGIITNVMNIIQGLWTLITIAFQ
                     AIGTVISVAVQIIVGLFTALIQLLTGDFSGAWETIKTTVTNVLDTIWQYMQSVWESII
                     GFLTGVMNRTLSMFGTSWSQIWSTITNFVSSIWNSVTSWFSRVASSVAEKMGQALNFI
                     ITKGSEWVSNIWNTVTSFASKVADGFKRVVSNVGDGMKNALDKIKSFFSDFLNAGAEL
                     IGKVAEGVANAAHKVVSAVGDAISSAWDSVTSFVSGHGGGSGLGKGLAVSQAKVMATS
                     FGKTFTSELGSTLTDGFNDSLTPSVDGHMTNDVQHSMKENNRPIVNVTVRNEGDLNMI
                     KSHIDDMDAKDGSFNLM"
     gene            12801..13736
                     /locus_tag="RJE42_000938"
     CDS             12801..13736
                     /locus_tag="RJE42_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDV5876381.1"
                     /translation="MIAHDVEIIKNGVKYRVSDNPHTYKHLRVLDYNVIGSGYKRNYS
                     PLDGVDGRFHNYAKEEYKKVELRLRYEVPKIAYASHLKSDIQTLFYGRFYLRELATPD
                     NTIKFENMFEPLEQEFELDYVDGRQLFVGLVSEVSFDTTKTSGEITLTFETTELPFFE
                     SIGYSTDLESDNDLEKWSVPDRIALNENDRSRQMTFYNTSSGDVYYNGDVALTQFNQF
                     NIVEIELAEDVKADDKDGFTFYMDKGNISVIKDVDLKAGDKIIFDNKHTYRGNLNIDL
                     YNKTLEQPVLYPGWNHFKANRLMKKIVFRHKLYYR"
     gene            13747..15633
                     /locus_tag="RJE42_000939"
     CDS             13747..15633
                     /locus_tag="RJE42_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV5876382.1"
                     /translation="MPVLLKSLQGVGHAIHVNTKLNEKLNEDSTLDIDMIENASTFDA
                     IGAITKMWTITNVKGEDDLDEYVIVMLDKSTIGNKIKLSIKARQKELDDLNNSRIYQE
                     YNESFTGVEFFNTVFKGTSYKYVLHTKVDASKFEGLGKGDTRLEIFKKGLERYHLEYE
                     YEAKTKTFHLYDELSKVADYYIKSGVNADNVKIQEDASKCYTYIRGYGDFDGQQTFTE
                     AGLQFEFTHPLAQLIGKREAPPLIDGRIKKEDVLKKSMELVIKKSVTASISLDFVAQP
                     EHFPEANPRIGDVVRVAEPTIGYNDLVRIVEITTHRDAYNNIIKQDVVLGDFTMRDRY
                     RKAIHEATNYVKNVKTTKSDPAKYLRELNAKVNASLSINNELVKQNEKINAKVDKMST
                     KTVTTANGTIMYDFTSQSSIRNIKSIGTIGDSVARGSHAKTNFTEMLGKKLKAKTTNL
                     ARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHDYWAGVPIGTDKTD
                     TKTFYGAFCSAIEVIRKNNPNSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTLEDYV
                     NAQILACSELDVPVFDAYHTDYFKPYNPAFRKASMEDGLHPNEKGHEVIMYELIKDYY
                     SFYD"
     gene            15646..17544
                     /locus_tag="RJE42_000940"
     CDS             15646..17544
                     /locus_tag="RJE42_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876383.1"
                     /translation="MAYGLITSLHSITGEKVVAQHEYNYRLLDNGMSKLEKMFIYHQK
                     EEVYAHTANQIKYLNGSVSDFLVYLNGRFSNMVLGHNGDGINEVKDARVDNTGYAHPT
                     LQDRLYHDYATLDELTKTVKKSVDDNYKEYRATEYRFEPKEQEPEFITDLSPYTNAVM
                     QSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIGNEL
                     WIYSAVLDANNNNKFVRFKYRSGEMTYGNEMQDVMPNVFNDRYTSAIYNPVENLMIFR
                     REYKTSEQQAKNALNFVEVRSADDIDKGIDKVLYQMDIPMQYTSGTQPMQGIAYDAGV
                     LYWYTGDSNPANPNYLQGFDIKKKELLFKRRIDIGGVNNNFTGDFQEAEGLDMYYDLE
                     TGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPVQNP
                     AYLSDVTEVGNYYLYSQDTQNALDFPLPKEFRDAGWFFDVLPGHYNGALRQVLTRNST
                     GRNMLKFERVIDIFNKKNNGSWNFNPQSAGFWEHIPKSITKLSDLKIVGLDFYITTEE
                     SKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLLRNFGTGGVGKWS
                     LFEGKVVE"
     gene            17544..19367
                     /locus_tag="RJE42_000941"
     CDS             17544..19367
                     /locus_tag="RJE42_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDV5876384.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNKPLSISKHNAMTSIVLKTDNFDDEHGAYISDELTIVDAINGRMQYVIPNEFLK
                     YTGRVHAQAYFTQNGSNNVIVERQFSFNIENDLISNFDGKTKLVYIKSIQDLTESVKE
                     EVEDLKKSLNDTKSLVSEIDSRINQGIQRLEIKQNEAVQMITTTQDKAVQYINTEFQK
                     IVDKEQAIYKRLSEVEKQINQSDLVKGSSTVNWQKSKITDDYGKAVESSEQSIDSVLS
                     AVNTSRIIHITSATDAPSFEDIGTVDTPKEDGVDDGSDIPVAPNTLGKSGVLVVYVVD
                     DSTARATWYPDDSNDEYTKYKIGGTWYPFYKKNDGDLTKQFVEETSNNALNQAKQYVD
                     DKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPSGVESYDGY
                     LSVFVKDETNKLFNFTPYNSKKIYTRSITNGRLEPQWTVPNEHKSTVLFDGGANGVGT
                     TINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECL
                     LSKTSSTTLRIDNDVYFDLGRTTGSGANANKVTITKIMGWK"
     gene            19367..19744
                     /locus_tag="RJE42_000942"
     CDS             19367..19744
                     /locus_tag="RJE42_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDV5876385.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     IFTNGEIKYNSNFEKEDVPNASSQQSASDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            19748..19921
                     /locus_tag="RJE42_000943"
     CDS             19748..19921
                     /locus_tag="RJE42_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDV5876386.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPEAKTSESQV"
     gene            19960..20394
                     /locus_tag="RJE42_000944"
     CDS             19960..20394
                     /locus_tag="RJE42_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876387.1"
                     /translation="MKNNIKDLTFSEIVGAIMLFSFGFRILFRGVFWVKEQGDVLDDS
                     DFYLALHHVMPIWIWGILVGLAGLITIVSSIFLASSDENNRCSWLLLVGGALTGVLYF
                     LMTSASVYHAINWLSTVHFAVMSTTGFIVAFIGGADIYARRK"
     gene            20378..20773
                     /locus_tag="RJE42_000945"
     CDS             20378..20773
                     /locus_tag="RJE42_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876388.1"
                     /translation="MQEESNFVSKHEFTKANGKIYERINEIDRKHTESLGELKVKVET
                     QTAVQKQSYEAQKETNANIKDLTKVMTDVSSEMKDIKYKVQGHDEKIKAIQGIIDSKI
                     KGSSQIITTLITTTGGVIVAAIGVAKFFF"
     gene            20826..22700
                     /locus_tag="RJE42_000946"
     CDS             20826..22700
                     /locus_tag="RJE42_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDV5876389.1"
                     /translation="MGLPNPKTRKPTASEVAEWAKSNIGKRINIDGYRGSQCWDTPNF
                     IFKRYWGFVTWGNAKDMANYRYPKGFRFYRYTSGFVPEPGDMAVWHPGNGIGSDGHTA
                     IVVGPSNKSYFYSVDQNWVNSNSWTGSPGALIRHGYASVTGFVRPPYSKDTSKPSNTD
                     TSSASKADDSTITGEAKKPQFKEVKTVKYTAYSNVLDKEEHFIDHIVVWGEERSDIQG
                     LYIKESTHMRSVDELYTQRNKFISDYEIPHLYVDREATWQARPTGFDDPRYPNWLVIE
                     VCGGQTDSKRQFLLNQIQALIRGVWLLSGIDKNLSETTLKVDPNIWRSMKDLINYDLI
                     KQGIPDNAKYEQVKKKMLETYIKRDILTRENIKEVTTKTTIRISDKTSVDSASRRGPT
                     PSDEKPNIVTETSPFTFQQALDRQMSRGNPQKSHTWGWANATRAQTSSAMNVKRIWES
                     NTQCYQMLNLGKYQGVSVSSLNKILKGKGTLDGQGKAFAEACKKNNINEIYLIAHAFL
                     ESGYGTSNFASGRYGAYNYFGIGAYDNNPNYAMTFAKNKGWTTPAKAIMGGASFVRKD
                     YINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFTRDKYK
                     "
     gene            22713..23303
                     /locus_tag="RJE42_000947"
                     /pseudo
     CDS             22713..23303
                     /locus_tag="RJE42_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
BASE COUNT         9024 a         4027 c         5297 g         6735 t
ORIGIN      
        1 agttaagacg attggatata gaatacactt tttaaattta tatccggtct tatacgaagc
       61 aaagaaggta gataaacaat gattaaacaa atattaagac tattattctt actagcaatg
      121 tatgagctag gtaagtatgt aactgagcaa gtatatatta tgatgacggc taatgatgat
      181 gtagaggcgc cgagtgactt cgcaaagttg agcgatcagt ctgatttgat gagggcggag
      241 gtgacagagt agatgtatag caaagagtca attgttaata tgataggcac acataaaatg
      301 aagtgtaatg tgttagctga tgtaataccg gaatatgata gcaattcaat cgcacagtat
      361 ggtatacaag cgacgttacc gaaaccacaa ggggaaaact cgagtaaagt agaagatgtt
      421 gttgtgaggc tcgagagagc aaataaaaga tatgcacaga tgttaaaaga ggttgagttt
      481 ataaatcaat cacaacagag attgggacac gttgactttt gtttcttaga gttattgaag
      541 aaaggttata acagggatgc gattatcaag aagatgccta actctaaatt aaatagaaac
      601 aacttcctag cacgccgtga tgagttggca gaaaagattt atctactaca gtgacgaaaa
      661 tgacaaaaat gacggaaatg acgaaaatga cactattttt aatccgtgaa ttaattttat
      721 ataattgatt tgtaagaatt atcttaagac gtggggtaat agccacatta gatgttctca
      781 tcgatgtgat tgagaagtga caaacatgta aagttgatat gttacgctat taatcactta
      841 ctacctgcct atatggtggg tagtttaatt cttgcaattt gagtcataac tattttcctc
      901 ctttcacatt tattgaacga agctcctgca caagatgtag gggcattttt ttatatttaa
      961 aataactaga gtaattaaca taaagtgggt ggtattatac gatgagtaat atgcaaaata
     1021 acgcaacatt tggcgcgtat ttagaattaa cgaagaaaca acaagcctat atacaaataa
     1081 agacagacac tggcgcaaaa gatatggata tcgcagaaca aattgacgtg aaccgcgcta
     1141 ctatatcaag gtggaaaact aatgataaat tcagggaagg ttttaaaggg tatcaagctg
     1201 agcatttgca aaaacaagtg cccaaagctc tacaaacaat gattaattta ttggacgcta
     1261 aaagtgagct tgtgaggttc caagcttcta aagatatttt agatcgtaca ggttataatc
     1321 ctgtcgacaa acaagaaata acaatgcaga gcgcggtgac gtttaatgac gatatcgatt
     1381 aacttatccg acttgctacc taaacatttc cacccattgt ggaaagtgac aaaggacaaa
     1441 gaagtactta atgtcgttgc aaagggtgga cgtggctcag gtaagtcatc agatatatca
     1501 atcatcatta cacaacttat tatgcgttat ccgatgaatg cggttgttat acgtaagaca
     1561 gataacacat tagctacatc agtgtttgaa caaatcaagt gggcaatcga agaacaaaag
     1621 gtgtcacact tattcaaagt taaagtgtcg ccaatggaaa taacttatat acctagaggg
     1681 aatcgtatta tctttagggg ggcacagaac cctgaacgat tgaagtcgtt aaaagatagt
     1741 aggttccctt tttctattgc gtggatagaa gagttggcag aattcaaaac agaagatgaa
     1801 gtcacaacga ttaccaactc attattacgt ggagaattag acgagggatt gttttataag
     1861 tttttcttta gttacaaccc acctaagcgc aagcaatcgt gggttaataa aaaatatgaa
     1921 agctcattcc aagctgataa cacattcgta catcattcta catacttgaa taacccgttt
     1981 atatcaaaac agtttataca agaggccgag agtgctaaaa agcgtaacga acaacgttat
     2041 cgatgggaat atatgggcga agcgataggg agtggtgttg tgccgtttaa taatttaaga
     2101 attgaagaaa taccgcaaag acaatatgac acattcgata atataagaaa tgcagtcgat
     2161 tttggttatg ctactgaccc attagctttt gtacgttggc attatgataa aaagaaacgt
     2221 gttatttacg caatggatga gtgttacggt gtgcaaataa gtaaccgtga gtttgctaat
     2281 tggcttaaaa agaaaggtta ccaaagcgat gaggtattcg ctgatagtgc tgaacctaaa
     2341 tcaatcgctg aattgaaaca agagcatgga attaaaaaga ttaaaggtgt aaaaaaaggt
     2401 gctgacagtg tggaattcgg agagcaatgg cttgacgatt tagatgctat tgtaattgac
     2461 ccaagacgca caccaaatat tgctagagag ttcgagaata tagactatga gacggacaaa
     2521 gacggcaatg ttaagcctaa attagaagac aaagacaatc acactataga tgcaacaaga
     2581 tacgctctag aacgtgatat gaggcaaaac aaactaagta tattgaggta ggtgtttaac
     2641 attattcgta tgccatggga taaaccatac ggcgaggaag tcgtcgaaca gttaaaaccg
     2701 caattcgaaa cacaagagga aatgattgtc agattaattg atgaccatag aaaacaatta
     2761 gataaaatta cagttggaca aaggtattac gacaaagaca acgacatcgt taagcaaatg
     2821 aaaaaagtag atgtgcacgg caatatcgat tatgataagc cggattggcg tattactacc
     2881 aactttcatc aaaacctcgt tgaccaaaaa gtcagttatg ttgctagtaa accagtaaca
     2941 tattcatgtg aggacgagaa tgtgttaaaa gtaattcatg atgtgctaga tacacgttgg
     3001 gataataagt tgatagatat cttgactgct acaagtaata aaggtatcga ctggctacaa
     3061 gtttacatta atgaaaacgg agaaatgaaa ctattccgcg ttccagctga acaagctata
     3121 ccaatttgga ttgataaaga aagagaagaa ttaaagtctt tcattcgtta ctacaagttt
     3181 aataacgagg aaaaagtgga gttttggact gatgctactg ttacttatta tgtattagag
     3241 aacggcggat taataccaga ttattattat ggtgctaatc acgttcaatc acatttcagt
     3301 aatggtaatt ggggtagagt accgtttata gcgtttaaga acaatcctga agaagtgtca
     3361 gacatatgga tgtacaaatc aataattgat gcaatcgata aaagattatc tgacgcacaa
     3421 aacatgttcg atgaatcagt tgaactcatc tatatcttaa aaggttatga gggcgaagac
     3481 ttagaagaat tcatgcgtgg acttaagtac tataaagcta tcaatgttga tggagacgga
     3541 ggtgtagaga cgatacaagt tgaagtgccg gtctcgagca ccaaagaata cattgacctt
     3601 atgagagctt atattatgga atttggacaa ggtgttgact tccaaactga taaattcggc
     3661 tcagctccaa gtggtatcgc actaaaattc ttatatggca acttagattt gaaagcaaac
     3721 aaacttaaga ataaagcgac tgtagcgata caagagttaa ttggttttat catagacttt
     3781 aataacttga agatggacgt taaagacatc gagatatcgt ttaactttaa cagaatgatg
     3841 aatgatgctg agcaatcaca aatcatcgcg caatctcaat atttatctag agaaacgctt
     3901 gtgaagagtt ctccgcttgt tgatgattac aaagcagaac tcgaacgaat agaacaagaa
     3961 caaatggagt acaacaagca actgcctaat ttagatgacg gaggtgctga cggtgcccaa
     4021 caacaagaaa gatctaacga taaacaatca gaatgatatt gatgaatata tcgatagtct
     4081 aatctctaaa gctgagaaac ccatagaaca actatttgct aatcgactta aagagataaa
     4141 acaaatcatc gcagatatgt ttgagaagta tcaaagtgat gatatgtatg ttacatggac
     4201 tgaattcaat aaatataaca ggctcaataa ggagttaacc cgtataggca caatgttgac
     4261 tgatgactat aggcaaatag ctaagatgat tcagaagtcg caggaagacg cttatataga
     4321 aaagttcctt atgagccttt atttatatga gacggcgagt caaacatcta tgcaatttga
     4381 tgttcctagc aaagaagtta tcacatcggc tattgaacaa ccaattgagt tcattcggtt
     4441 agtaccgacg ctacagaagc atcgtgatga agtactgaaa aagatacgct tacatatcac
     4501 acaaggcatt atgagtggag agggctactc taagatagcg aaagcaatcc gtgatgatat
     4561 tggtatgtct aaagctcaat cgttgcgtgt agctcgtaca gaagcgggaa gagcgatgtc
     4621 tcaagctgga cttgatagtg cattagtagc tcaaaagaac ggcttacaga tgtataagta
     4681 ttggcaagct actaaagata cgcgtacaag ggacacacac aggcatctag acggtgctaa
     4741 gaagaaaata gacgaaccgt tcaagtcgag cggttgcgtt gggcaggcac ctaagttgtt
     4801 cgttggtgtg aatagtgcga aagaaaacat taactgtcgt tgtaagctta tgtattacat
     4861 tgatgaagat gatttaccta gtacaacaag agtacgtaaa gatgatggca caactgaagt
     4921 aacaccacaa atgacttatc gtgagtggga gaaatataaa cgtaaaagaa agtagtttac
     4981 tactcgacct tagcatgtcg ttaaactgct tctttttata ccaaaattct tcgtggcgtt
     5041 gcacgtaaaa ctcgtaaaaa ggagtagttt aaatggattt atacacattg ttaggacaat
     5101 ttaaagacgg agaaatcgac aaacagaagg taattgatgc gattgacgaa tcaaaatcgg
     5161 gaatggtacc acgttcgaga ttgaacgaca agaataccga aattgaagag ttaaaagaag
     5221 agatttctaa acgtgatgaa caaattgtca aattgcaaga ctctgttaaa gatgatagcg
     5281 agattcaaaa agaactcgaa gaattaaaga atcaaaattc agagtgggag acaaagtata
     5341 aagaaacaca acttaataac gcagttaagt tagcggttgc taaagaagca aatgatgcta
     5401 acgacattct agcattcatc aataaagatg aactggaatt agtagacgac ggcactgtaa
     5461 aaggtttaga tgaagcaatt aaaacgctta aagagtccaa accttattta tttgcgtcgt
     5521 ctaagcctgt aggtaaaaca ccacaaggcg gaggtaatcc agactcaggt gtaacgaaag
     5581 aaaagtttga caacatgagt gtcgctgaac gtaacgaatt gtatttgaac gatcgtgaga
     5641 cattcgaaaa attagttaat caaaattaaa caaagaaaga ggtacaagca tggcacaagg
     5701 acttactaaa acaagtaatc aaatcattcc agaagtatta gcgcctatga tgcaagcaca
     5761 actcgaaaag aaattgcgtt tcgcttcatt tgcagaagta gataacacat tacaaggaca
     5821 accgggagac actttgacat tcccagcatt tgtttatagc ggagacgcac aagtagttgc
     5881 agagggagaa aaaatcccta ctgatatttt agaaacgaaa aaacgtgagg ctaaaattcg
     5941 taaaatcgct aaaggtacat ctatcacaga tgaggcttta ttaagtggtt atggagaccc
     6001 tcaaggcgaa caagtgcgtc aacacggttt agcacacgct aacaaagttg ataatgacgt
     6061 attagaggct ttaatgggag ctaaacttac tgttaatgcg gacatcacta aattaaacgg
     6121 cttacaatca gcaatcgaca aatttaacga tgaagactta gaaccaatgg ttttatttgt
     6181 taatccactt gatgctggta aattacgtgg agatgcatca actaacttta cacgcgcaac
     6241 cgaattaggc gatgacatca tcgttaaagg cgcgtttggc gaagctttag gcgctatcat
     6301 tgtacgttct aataagttag aagctggcac ggctatttta gctaaaaaag gcgcagttaa
     6361 attaatcttg aaacgtgatt tcttcttaga agtagcgcgt gacgcatcaa caaaaacaac
     6421 tgcattatac agtgataagc actatgtagc ttatttatat gatgaatcta aagcagtgaa
     6481 aatcactaaa ggttctggaa gcttagaaat gtaataggag gtagtgacgt atgtataaag
     6541 taatcgaacg ttttgaagat gcacaagaca acggacgtga atatcaagtg ggagacattt
     6601 acccacgtga tggattggaa gtatcagaag aacgattcac tgaattatct acaacaaata
     6661 accgccgtaa tttaatcgct atcaaacttg ttgaagaaaa gcagacagaa cagtctgagg
     6721 cgaacgctga ggagcaaaaa agtttgtctg atatgaaagt ggcagaatta aaagaacttg
     6781 ctaaaaagcg tgaaattaaa ggttatagcg atatgaaaaa agatgagctt atcaaagttt
     6841 tagagggtgt taagtaatgg acacaaaaga cgtcaaaatg attaatggac tttcactcaa
     6901 tgattcgtct aacgatgagc aaatcgaata tcttattgaa gaatataaaa gtgttgcaga
     6961 agattattgt aatcagaagt ttgatgacaa agaagtgccg tcgggtgtta agaagtttat
     7021 tgctgaatgt atcaagtttg gtacaaccgg caatatctca gcgcgcacga tgggcactgt
     7081 gagttatacc tatgtaactg atatacctag tagtgcttac gcttatctaa tgccttatcg
     7141 taaattaagt tggggtaagc gatatgttta atccgtttga tgagtttccg cacacaattg
     7201 aaattggaga ggttgaagtc gtaggaacat atcccaaaga atacgagcgt tttaaaagta
     7261 acgaaacaat taaagggttt atggacacac ctacatcaag tgagacactc aaatttcatc
     7321 agatgagcaa agatttcgac cgaaacctat atacgccgta ccacatacca ataacaaaca
     7381 aaactttatt taattacgag ggtaaaacgt acgaagttgt aggcgaaccg gtcgaccaag
     7441 gcggacaaca tgaaattaat ttaactagat tgagggtgcg atctattggc aaaggttaaa
     7501 tatggtaatt gggacttagt aaaagagttg gaaaattatg agcgagacat agaacgatgg
     7561 gtcaaacgag gtatagcaaa gactactact aaaattcaca atacaatcat ttcattaatg
     7621 ccagttgata ccggctatct tagggaaagt gtaacaatgg actttaaaga tggcggatta
     7681 actggcgtta ttaacattgg tagtgaatac gcaatatatg tcaattatgg tactggtata
     7741 tatgcaacag gtgctggagg tagtagagcg aaaaagatac cgtggtcgta caaagatgcg
     7801 aacggtaagt ggcacactac taaaggacaa catgctcaac ctttttggga gccggcaata
     7861 gacgctggac gagcgttctt taataagtat ttttcatgag gtggttaata tatgtgggta
     7921 tcagttgaac ggtacttatt taacaaagta tataacaaat taaaaagtaa ccctattatc
     7981 caaaaacaat tgggtggtag ggtttttgat tgcgttcaga aagacgctgt ttacccatat
     8041 atcgttgtgg gtgaaacaaa cgtcactaac aaagaaacga ccacgagcat ggtcgaagat
     8101 gtcggcatca cgttacatgt ttatagtcaa gcgcgtaata gagatgaggc atcacaaaca
     8161 attcaatttt taggctttgt tttaaacaac gaaatagaaa ttgattatta ttcattcatt
     8221 aaaagtcgaa ttgatacaca agaagtgatt actgacatag atcagtacac taaacacggt
     8281 atcattcggc ttgtttttaa atacagacat aacacattac aaaggagtgt aacgaatggc
     8341 gcaggataaa tatattgtcg ctctccaaat cgctgataaa gatttagcta agaagctaac
     8401 tatcgaagaa gcaacgcttt taggtagttt agcagagggt gggcacacta tcagtaatga
     8461 ccttgctgaa atcattcaag gcggtaagaa agattatagc cgtaactctg tcgaagaaga
     8521 aatcaagttg acgcttgatg tcgttccggg agataaagga caattagcat taaaagaatc
     8581 agttaagcaa ttcaaacagt tacgtgtttg gatttgggaa actaaaaaac gcgatggcaa
     8641 acatcacggt gtattcgcat atgtagttat cgaagagcac gaatggtcat ttgatgatga
     8701 agataacaaa atcgaaatca cagcgaaagt taagttcaat agtgcagacg gtacaatcaa
     8761 tgatttacca aaagaatggc ttaaccctag cgcattggct ccagttgttg aattcgaaga
     8821 tatgaacgct tacgaagata gttatgaaaa ccgaactaaa aaaacaactg ctggcagtag
     8881 cgatttaagt atgtaattaa cgagggcatt agccctctat ttttttgtac aaaataacga
     8941 taaacgaggt atttaatatg actgaaacaa cttttaatcc gattacatca ttaacaatta
     9001 acaatgaaga agtaaaagca aaagcaacat ttatgttcga taaaaccgct aaaaagtttg
     9061 cgactgaaca agaggataac aaaggcagaa aacaaacaat ttcaggattt actaatgttt
     9121 ataacgcttt attagaacgt gacacagtag caattgtaga cttttgggaa tgtgcaacgg
     9181 cttacctagg taaaagcgca cctaaaagag aagatattga agcggaaatc atggaaatca
     9241 tcgaaagaga aaacgacacg ttaaatctat tacaaggtgc gttggacgta atgaataata
     9301 gcggtttttt caagcagaaa tcacgtctat tctggacaca gatgaaccaa gcgccatcgt
     9361 tagccaaaga agacgagaaa gagggcgcga aagctggtat cgagatgatg aagaacaact
     9421 acaaagaaat catgaccgta gcaccttatt agactattcg gaaataaggc agatgacaag
     9481 tcgttacata ggttatatga gtaatgacga gttaatgagc atgctacctg ccgaatggaa
     9541 tgactggatt attggggcta gacaagcatt gattgaccaa agggacattg cgttgtacgg
     9601 cgctcaatat aatgcggttg ctcaagctgg taaatcacta aaacgttttg ttaggcagaa
     9661 cgaaagagaa cattacatta ttcgtggtca agaaggtgaa tacgaaagaa tgaaacagcg
     9721 tgagctagct aaaaacaaac gtaaaagaga aatacaaaaa caagggactc gcaagttcct
     9781 taacagctta aaaacaagtc ataaaggagg ttaggcatgg aaaagaattt tctggctcgt
     9841 gttacagcta taatcagtga ttttaaaagg aacatcagaa ctgctcaacg tatggctaag
     9901 actgatatac cggacgaaat caagacagaa gttacagcta acataagaga ttaccaaaga
     9961 gagctaacgc gagccaaatc gatggctcaa cgttggcgag aacataaagt taatatcgat
    10021 gcagacgcta gtaaagtaaa acaagtcata tcgtttgtta aagcagaact atcgaatatc
    10081 agacgtaaaa aagttgaaat tgacggcgac gcaagcggat taaaaagaaa tgttacgact
    10141 tctaaagcaa tgttagctgg ttggcgcaaa cacactgtta aattagattt tgatacaacc
    10201 ggaatgacga aaatgcaagt agcattgacc gctggtaaaa gagcgttaga gcagtatcaa
    10261 tcaacaatgg atggcatcgc atcaaatatt agaactttcg gtactatctt tgcgcaacaa
    10321 gtcaaaggtt taatgattgc tagtatacaa gcgttaatac caataattgc tggattagtt
    10381 ccggctatta tggcagtact taatgccgtt ggtgtattag gtggtggcgt catcggtcta
    10441 gctggtgcgt tctctgtagc aagtgttgga gcggttggct tcggtgcaat ggctattact
    10501 gcactaaaaa tggtaaaaga tggaacatta gcagtaacaa aagaagttca aaactttaga
    10561 gatgcgagcg atcagttaaa aactacatgg caaggcattg taaaagagaa tcaagcaagt
    10621 atttttaatg cgatgtcagc gggtatcaga ggcgttacaa gtgcgatgtc tcaattaaaa
    10681 ccattcttat ccgaagtatc tatgctggtt gaagcaaacg cacgcgagtt tgagaattgg
    10741 gttaaacatt ccgaaacagc taagaaagcg tttgaagcat tgaatagcat aggtggcgca
    10801 atcttcggag atttattgaa cgctgcagga cgatttggcg acggattagt taacattttc
    10861 acacaattaa tgccgttatt caaatttgtg tctcaaggac tacagaacat gtctatagct
    10921 ttccaaaatt gggctaatag tgtagctggt caaaatgcta ttaaagcgtt tattgactac
    10981 actaccacta acttacctaa gattggtcag atatttggta atgtattcgt tggtattggt
    11041 aatttaatga ttgcttttgc acaaaacagt tccaacattt ttgattggtt agttaaatta
    11101 acttctcaat tcagagcgtg gtcagaacaa gtagggcaat cacaaggatt taaagacttt
    11161 atcagttatg ttcaagagaa tggtcctact attatgcagt taatcggtaa tatcgtaaaa
    11221 gcgttagttg cttttggcac tgcaatggct cctatagcta gtaaattatt agactttatc
    11281 actaatctag ctggatttat cgctaaacta ttcgaaacaa acccagctat agcacaagtt
    11341 gctggcgtta tgggtatttt aggcggtgta ttttgggctt taatggctcc gattgttgct
    11401 ataagtagtg tgcttacaaa tgtgtttggt ttgagtttat ttggagttat caaacaaatt
    11461 ttaagcttcg ttagaacatc aagtctagtt actggagcta tgcaattgtt aatgggcgtt
    11521 ttcggttcga tttcagcacc tattttagca gttatagcag taattggtgc attcattggt
    11581 gtcctagttt atttatggaa aacaaacgag aatttcagaa aaactattac tgaagcatgg
    11641 aacggtgtta aaacggcggt ttccggtgcg attcaaggtg tagttggttg gttaactgaa
    11701 ttgtggggca aaatccaatc taccttacaa ccgataatgc ctatattaca agtattagga
    11761 caaatattca tgcaagtttt aggtgtgttg gtaataggca tcattacaaa cgttatgaat
    11821 atcatacaag gtttgtggac gttaattaca attgcgttcc aagccatagg aacagtgata
    11881 tccgtagcag tccaaatcat agtaggtttg ttcactgctt tgattcagtt gcttactggc
    11941 gacttctcgg gtgcttggga gactattaaa actacggtta ccaatgtact tgatacgatt
    12001 tggcaataca tgcaatcagt ttgggagtca attatcggct ttttaactgg cgtaatgaat
    12061 cgaacacttt ctatgtttgg tacaagttgg tcacagatat ggagtacaat cactaatttt
    12121 gttagcagta tttggaacag tgttacaagt tggtttagtc gtgttgcttc aagtgtagct
    12181 gaaaaaatgg ggcaagcact aaactttatt atcacaaaag gttccgaatg ggtttctaat
    12241 atttggaaca cagttacaag tttcgcaagt aaagtagctg atggatttaa aagagttgtc
    12301 tcaaatgtag gagacggcat gaaaaacgcg cttgataaga ttaaaagctt tttcagcgat
    12361 tttttaaatg ccggagcgga attaatcggc aaagtggcag aaggtgtagc taacgctgcg
    12421 cacaaagtag taagtgctgt aggcgatgcg atttcatcag cttgggactc tgtaacttca
    12481 ttcgtgagtg gtcacggtgg tggtagtggt ttaggtaaag gtttagcagt atcacaagct
    12541 aaagtaatgg ctactagctt cggtaaaaca tttacaagtg agttaggttc aacgttaaca
    12601 gatggcttca acgatagttt gactccaagt gttgatggtc atatgacaaa cgatgtgcaa
    12661 catagcatga aagaaaacaa tagacctatt gttaatgtga ctgttaggaa cgagggcgat
    12721 ctaaacatga ttaaatctca cattgacgat atggatgcaa aagatggtag tttcaactta
    12781 atgtaaggga ggtttgttta ttgatagccc atgatgtaga aattattaaa aatggtgtga
    12841 agtatcgagt cagtgacaat cctcatactt acaaacactt aagagtgctt gattacaatg
    12901 ttatcggttc aggttacaaa aggaattatt cgcctttaga tggtgttgac ggacgttttc
    12961 acaattacgc taaagaggaa tataaaaaag ttgaattaag attgaggtat gaagtaccta
    13021 aaattgctta tgcctcacat cttaaatcag acattcaaac attgttttat ggtcgctttt
    13081 acctaagaga attggcaacg ccggataaca ctatcaaatt tgaaaatatg ttcgaaccat
    13141 tagagcaaga atttgaatta gattatgttg acggtagaca actattcgtt ggattagtta
    13201 gcgaagtatc tttcgacacg actaagacat caggagaaat cacgttgact tttgagacga
    13261 cagaattgcc tttctttgaa agtattggct atagcactga tttagaaagt gataacgatt
    13321 tagaaaaatg gtcagttccg gacagaatag cactaaatga aaatgataga agtagacaaa
    13381 tgacattcta taatacgagt tctggagatg tttattacaa cggagatgta gcattaacac
    13441 agttcaacca attcaatata gttgaaattg aattagccga agacgttaaa gctgatgata
    13501 aagacggttt cactttctat atggataaag gaaacatctc agtaattaaa gatgtcgatt
    13561 taaaagcagg cgataaaatc atttttgata acaagcacac atatagaggc aatttaaata
    13621 ttgacctata caacaagacg ttagaacaac cggtgttgta tcctggttgg aatcatttta
    13681 aagccaacag acttatgaaa aagatagtct ttagacacaa attatattac agataaggag
    13741 tagcatatgc cggtattatt aaaaagtttg caaggcgtcg gtcatgcgat tcatgttaat
    13801 acaaaattaa acgaaaaatt gaatgaagat agcacgttag acattgatat gatagaaaat
    13861 gccagcactt tcgacgcaat cggcgctatt acaaagatgt ggactatcac aaacgtaaag
    13921 ggggaagatg acctcgatga atatgtgata gtaatgcttg ataaatcaac aattggaaac
    13981 aaaatcaaac ttagtatcaa agcgagacaa aaagaattag atgatctaaa caattctagg
    14041 atttaccaag aatataacga aagtttcaca ggcgtagagt tttttaacac tgtatttaaa
    14101 ggaactagtt ataagtacgt attgcacact aaggttgacg catcaaagtt cgagggatta
    14161 ggtaaaggag acacaagact tgagatattc aaaaaaggtc ttgaacgtta tcatctcgaa
    14221 tatgaatacg aagctaaaac caagacattt catttgtacg atgaattatc taaagtagca
    14281 gattactaca tcaaatcagg tgtaaatgct gataatgtca aaattcaaga agatgcttcg
    14341 aaatgctaca catatataag aggttatggc gactttgacg gtcaacaaac ctttacagag
    14401 gctggattac aattcgaatt cacacaccca ttagcacaac tgattggtaa aagggaagcg
    14461 cctccgttaa tagatggacg cataaaaaaa gaagatgttt tgaaaaaatc aatggagcta
    14521 gtgataaaga aaagtgtcac tgcttctatt tctttggact tcgtagcaca gcctgagcat
    14581 tttccagagg ctaaccctag aattggcgat gtcgtaagag tggctgaacc aactataggc
    14641 tataacgact tagtgagaat agttgaaatc actacacata gagatgcgta caataacatc
    14701 attaaacaag atgtagtatt aggcgatttt acaatgcgcg acaggtatag aaaagctatc
    14761 catgaagcta cgaactatgt taagaatgta aaaacaacta agtcagaccc agctaagtac
    14821 ttgagagaac taaacgctaa agttaacgct agtttatcta taaataatga attagttaag
    14881 cagaatgaaa aaataaacgc aaaagtcgat aagatgagta ctaaaacagt tacaactgcg
    14941 aatggcacga ttatgtacga ctttacgagt caatcaagta taagaaatat caaatctatt
    15001 ggaacgattg gcgattctgt agctaggggg tcgcacgcaa aaactaattt tacagaaatg
    15061 ttaggcaaga agttaaaagc taaaacgacc aaccttgcaa gaggtggcgc aacaatggca
    15121 acagttccaa taggtaaaga agcggtagaa aacagcattt ataggcaagc agagcaaata
    15181 agaggcgact taatcatatt acaaggtaca gatgatgact ggttacatga ttattgggca
    15241 ggcgtaccga taggcactga taaaacggac actaaaacgt tttacggtgc cttttgttct
    15301 gcaattgaag ttattagaaa gaataatccg aattcaaaaa tactagtgat gacagctaca
    15361 agacaatgcc ctatgagtgg tacaacgata cgccgtaaag acacagacaa aaacaaacta
    15421 gggttaacac ttgaggacta tgtaaacgct caaatattag cttgtagtga gttagacgta
    15481 ccagtgtttg acgcatatca cacagattac tttaagccat acaatccagc ttttaggaaa
    15541 gcgagcatgg aggacggctt acaccctaac gaaaaaggtc acgaggttat tatgtacgag
    15601 ttaatcaagg attattacag tttttacgac taaaggaggc aaccaatggc ttacggatta
    15661 ataacaagtt tgcattctat cactggcgaa aaagtagttg ctcagcatga gtacaactat
    15721 cgattacttg ataatggaat gagtaaactt gaaaagatgt ttatatatca tcaaaaagaa
    15781 gaagtatatg cgcacacagc taaccaaatt aaatatttga atggtagtgt ctcagacttt
    15841 ttagtttatt taaacggtcg attcagcaac atggtactag gtcataacgg cgacggtatc
    15901 aatgaagtaa aagacgcgcg tgttgataat acaggatacg cgcacccaac attacaagat
    15961 cgtttgtatc atgattatgc aacgttagac gaattaacca aaacggttaa aaagtcagtt
    16021 gatgataatt acaaagaata tagagctaca gaataccgat tcgaaccaaa agaacaagaa
    16081 ccggaattca tcactgattt atcgccatat actaacgcag taatgcaatc attttgggta
    16141 gaccctagaa caaaaattat ttatatgaca caagcgcgtc cgggcaatca ttacatgtta
    16201 tctagattga aacctaatgg acaatttatt gatagattgc ttgttaaaaa tggcggtcac
    16261 ggcacacaca acgcctatag atatatcggc aatgagttgt ggatttattc agcagtgtta
    16321 gacgctaaca ataacaacaa gtttgtacgc tttaaataca gaagtggaga aatgacgtat
    16381 ggcaacgaaa tgcaagacgt catgccaaac gtatttaacg atagatatac gtcggcaatt
    16441 tataatccag tagaaaactt aatgattttc agacgtgaat ataaaacttc tgaacaacaa
    16501 gctaagaacg cattaaactt cgttgaagtt agaagtgctg atgatattga taaaggtata
    16561 gacaaagtac tgtaccaaat ggatatccct atgcaataca catcaggtac gcaacctatg
    16621 caaggtattg cttatgatgc aggtgtctta tattggtaca ctggcgattc aaatccagct
    16681 aaccctaatt acttacaagg attcgacatc aaaaagaaag aattgttatt taaacgtcgt
    16741 atcgatattg gcggtgtgaa taacaacttt actggagatt tccaagaggc tgagggtcta
    16801 gatatgtatt acgatctaga aacaggacgt aaagctcttt taatcggggt aactattgga
    16861 ccaggtaaca acagacatca ctcaatttat tctatcggtc aaagaggtgt aaaccaattc
    16921 ttaaaaaaca ttgcacctca agtatcgatg actgattcag gtggacgtgt taaaccactt
    16981 ccagttcaaa acccagcgta tctaagcgat gttactgaag ttggtaacta ttacttatac
    17041 tctcaagata cgcaaaacgc gctagacttt ccgttaccta aagaattcag agatgcaggt
    17101 tggttctttg atgtattgcc tggtcactat aacggcgctt taagacaagt gcttactaga
    17161 aatagtacag gtagaaatat gctcaaattt gagcgtgtta tcgacatttt caacaagaaa
    17221 aacaacggtt catggaactt taacccgcaa agtgcaggtt tttgggaaca tattccgaaa
    17281 agtattacta agctatctga tttaaaaatt gttggcttag acttctatat cactacagaa
    17341 gaatcaaaga ggtttactga tttccctaaa gacttcaaag gtattgcagg ttggatatta
    17401 gaagtaaaat cgaatacacc aggcaacaca acacaagtgt taagacgtaa taacttcccg
    17461 tcagcccacc aattcttatt gagaaacttt ggtactggtg gcgttggtaa atggagttta
    17521 ttcgagggaa aggtggttga ataatggtag tagataattt ttcaaaagat gataacttaa
    17581 tcgagttaca aacaacatca caatataatc cagttattga cacaaacatc agtttctacg
    17641 aatcagatag aggaactggt gttttaaatt ttgcagtaac taagaataat aagccgttat
    17701 caatcagcaa acataacgcg atgactagta ttgtgcttaa gacggataac ttcgacgatg
    17761 aacacggcgc ttatattagt gatgaactta caattgtcga tgcaattaat ggacgaatgc
    17821 aatacgttat ccctaacgag tttttaaaat acactggacg cgtacatgca caagcttact
    17881 ttactcaaaa cggtagcaat aacgtaattg tagagcgtca atttagcttc aatatcgaga
    17941 atgatttaat tagtaatttt gacggtaaaa caaagctagt ttatatcaag tcgattcagg
    18001 acttaacaga aagtgttaaa gaagaagttg aggacttaaa gaaaagtttg aacgacacta
    18061 agtctctagt tagtgaaatt gatagccgta tcaatcaagg tattcaaagg ttagaaatca
    18121 aacaaaatga agcggtacag atgattacaa caacacaaga caaagccgtt caatatataa
    18181 atactgagtt ccagaaaatt gttgataagg agcaagcgat ctacaaaaga ttgagtgagg
    18241 ttgaaaagca aatcaaccag tctgaccttg tcaaaggaag ttcaacagtt aattggcaaa
    18301 agtctaaaat cactgatgat tacggtaaag cggttgaatc gtctgagcag tccatagata
    18361 gcgttttaag tgcagttaac acatctagga ttattcatat cacaagcgca acagatgcgc
    18421 cctcgtttga agatataggc actgtcgata cacctaaaga agatggcgtc gacgatggtt
    18481 cagatattcc ggtagctcct aacactttag gaaagtcggg cgtgttagtt gtctatgttg
    18541 ttgatgacag tacggcacgt gcaacatggt atccagatga ttcaaatgat gaatatacaa
    18601 aatataaaat tggtggtacg tggtacccat tttataaaaa gaatgacggc gatttaacta
    18661 agcaatttgt tgaagaaacg tctaacaatg ctttaaatca agcgaagcag tatgtcgatg
    18721 ataaattcgg aacaacgagc tggcaacaac ataagatgac agaggcaaat ggtcaatcaa
    18781 tacaagttaa cttaaacaat gcgcaaggcg atttgggcta tttaactgct ggtaattact
    18841 atgcaacaag agtgccggat ttaccaagtg gcgttgaaag ttatgatggg tatttatctg
    18901 tatttgttaa agatgaaaca aataagttat ttaacttcac accttataac tctaaaaaga
    18961 tttacacacg atcaatcaca aacggcagac ttgagccaca gtggacagtg cctaatgaac
    19021 ataagtcaac ggtattgttc gacggtggtg caaatggtgt aggtacgaca atcaatctaa
    19081 ccgaaccgta cacaaactat tctattttgt tggtaagtgg aacttatcca ggtggtgtta
    19141 ttgagggatt tggactaacc gcattaccta acgcaattca attaagtaaa gcgaatgtag
    19201 ttgactcaga cggtaacggt ggcggtatat atgagtgctt actatccaaa acaagtagca
    19261 ctactttaag aatagataac gatgtgtatt tcgatttagg cagaacaaca ggttctggcg
    19321 ctaatgcaaa taaagttact ataactaaaa ttatggggtg gaagtaatga aaatcacagt
    19381 gaacgataaa aacgaagtta tcggatacgt taatactggc ggattacgca atagtttaga
    19441 tgtggacgat aacaatgtgc caatcaaatt taaagaagag ttcgaaccta gaaagtttat
    19501 tttcactaac ggcgaaatta aatacaatag caatttcgaa aaagaagacg taccgaatgc
    19561 atcaagccaa caaagcgcat cagatttaag tgatgaggaa cttcgcggaa tggttgcgag
    19621 tatgcaaatg cagatgacac aagtgaacat gttaacaatg cagttaactc aacaaaacgc
    19681 tatgttaaca caacagttga ctgaactgaa aactaataaa actaatactg agggggatgt
    19741 ttaaatgatg aagatgattt atccgacttt taaagacatt aaaacttttt atgtgtgggg
    19801 ttgttacaaa aacgagcaaa ttaagtggta tgtggatatg ggcgttatcg acaaagaaga
    19861 atacgcatta atcactggag aaaaatatcc agaggcaaaa acaagtgagt cacaagtgta
    19921 atacttgtgg cttttaaatt tgaataaagt gggtgtgata tgaaaaataa tataaaagat
    19981 ttaacgtttt ctgaaattgt cggcgctatc atgttgtttt ctttcggttt cagaattcta
    20041 ttccgtggcg tgttttgggt taaagaacaa ggcgatgtgc tagatgattc tgatttttat
    20101 ttagccttgc atcatgttat gcctatttgg atatggggca tattggttgg tttagcagga
    20161 ttaatcacaa ttgtctcatc tatattttta gcttcaagtg acgaaaacaa tcgatgtagc
    20221 tggttgttgt tggtgggtgg tgctttaaca ggcgtattgt actttttaat gacaagtgca
    20281 agcgtttatc atgctattaa ctggctttct actgtgcatt ttgccgtaat gtcaacgact
    20341 ggttttattg ttgcatttat tggaggtgct gacatctatg caagaagaaa gtaatttcgt
    20401 ttctaagcac gaatttacca aagctaacgg caagatctat gagcgtatta atgaaataga
    20461 tagaaagcat acagagtcgt taggcgaatt gaaagttaaa gtagaaacgc aaacagcggt
    20521 acaaaaacaa tcatacgaag ctcaaaaaga aacgaacgca aatataaaag atttaactaa
    20581 ggttatgact gatgttagtt ctgaaatgaa agacattaaa tacaaagtgc aaggtcatga
    20641 cgaaaagata aaagcgatac aaggtataat tgattcaaaa ataaaaggta gctcccaaat
    20701 aataacgaca cttataacca caactggcgg tgtaatagtc gctgcgattg gagtagctaa
    20761 gttcttcttt taaaggtcag tgcttcggca ctggcttttt attttgattg aaatgaggtg
    20821 catacatggg attacctaat ccaaagactc gaaaacctac agctagtgaa gtagctgaat
    20881 gggcaaagtc taatataggc aaaaggatta atattgacgg ttatagaggt agtcaatgtt
    20941 gggatacacc aaactttatt tttaaaaggt attggggttt tgtaacttgg ggcaatgcta
    21001 aggatatggc taattataga tatcctaagg gtttccgatt ctatcgttat acatctggat
    21061 ttgtaccaga gcccggagac atggcagttt ggcaccctgg caatggaata ggttcagacg
    21121 gacacactgc aatagtagta gggccatcta ataaaagtta tttctacagt gttgaccaaa
    21181 actgggttaa ctctaatagt tggacgggtt cgcctggtgc gttaattaga catggatatg
    21241 caagcgttac aggatttgtc agacctccat actcaaaaga tactagcaaa cctagcaata
    21301 ctgatacaag ttcagcatca aaagctgatg actcaacaat tactggagaa gcgaagaaac
    21361 cgcaattcaa agaagttaaa acagttaaat acactgctta tagcaatgtt ttagataaag
    21421 aagagcactt tatagaccat atagttgttt ggggagaaga acgctcagac attcaaggtt
    21481 tatacatcaa agaatcaaca cacatgcgtt ctgttgatga attatataca caaaggaata
    21541 aatttattag tgactacgag ataccgcatt tatatgtcga tagagaagcg acgtggcaag
    21601 cgagaccgac tggctttgac gacccgcgtt atcctaactg gttagttatt gaagtatgtg
    21661 gcggtcaaac agatagcaaa cgacaattct tattgaatca aatacaagcg ctaatacgtg
    21721 gcgtttggtt gttgtcgggg attgataaaa acttatctga aacgacgtta aaggtagacc
    21781 ctaatatttg gcgtagtatg aaagatttaa ttaattacga cttgattaag caaggtatac
    21841 cggataacgc aaagtatgag caagttaaaa agaaaatgct tgagacatac attaaacgag
    21901 atatattgac acgagaaaat ataaaagaag taacgacaaa aacaacgata cgaattagtg
    21961 ataaaacttc ggttgacagt gcatccagaa gaggccccac tccgtcagac gaaaaaccaa
    22021 acatcgtcac tgaaacaagt ccattcacat tccagcaagc actggataga caaatgtcta
    22081 gaggtaaccc tcagaaatcg cacacatggg gttgggctaa tgctacacga gcacaaacga
    22141 gctcagcaat gaatgttaag cgaatatggg aaagtaatac gcaatgttac caaatgctta
    22201 acttaggtaa gtatcagggt gtttcagtta gttcacttaa taagatactc aaagggaaag
    22261 gaacactcga cggacaaggt aaagcattcg cagaagcttg taagaaaaat aacattaacg
    22321 aaatctattt gatcgcgcac gctttcttag aaagtggata cggaacaagt aacttcgcta
    22381 gtggtagata cggtgcttac aactattttg gtattggcgc ttatgacaac aatcctaact
    22441 acgcaatgac atttgctaag aataaaggtt ggacaactcc agcaaaagca atcatgggcg
    22501 gtgctagctt cgtaagaaag gattacatca acaaaggaca aaatacactg tacagaatca
    22561 gatggaatcc taagaatcca gctacacacc aatatgctac tgctatagag tggtgtcaac
    22621 atcaagctag tacaatcgct aagctatata aacaaatcgg cttaaaaggt atctacttca
    22681 caagggataa atataaataa agaggtgtgt aaatgtacaa aatacaagac attgaaacgc
    22741 gaataaataa aagaacagtt gatatcggcg acataggttg tcgctactac acggaagatg
    22801 aaaacacagc ttatgtcaga atcggtatca acgacgaaaa aggtagaatc gacttcaaag
    22861 aaagcaattt gacacctaag ttacatctat tcttggaaga cggttctata ttcaaaaatg
    22921 aacccgtttt aatcgacgat aatgtaaaag ggttccttac ctacaagata cctaaaaacg
    22981 tcattaaaca cgtcggtatg gtgcgttgta agttatttct agagaacgac cacgagagaa
    23041 tacacgtcgc aaacttccat ttctacatta tcgatagtgg tatagacgaa gcagtgcaaa
    23101 aagaggtgtc tatcacatta gttgaggaca ctgtaaaaag aattatccgt acaagcgcta
    23161 gcgagttact aggagacgac ttcaaagaaa ctttaaatac aactgctaag caatacatcg
    23221 ctgacaatgc agacaaattt aagggcgagc gtggcgagcg tggagaaaaa ggagaagcgg
    23281 gcgaacgagg cgctgagggt attataaggt ttgaaaattt aactcaagaa caacgaaatg
    23341 agttaaaggg agaagcgggc gaaaacatta ttaaagataa tgccgttact tataacaaaa
    23401 cggattttat caaaactggt aagaatattt ttgatactac cgatattgaa gtaggtagga
    23461 ttgttagcaa cacaacagga atagtaagtg atagcgctta ttatgtaaca agtaaatttt
    23521 tgccggtagc tcctaatacg gtatacacgc aaaattatgc agacccaatc gctttttacg
    23581 atatcaacaa aacgtttata tctggattag cgcgtgttac aaatccaaaa ggcgctagga
    23641 cttttaccac acctacaaac gcatattaca ttaaagcgac aagcattaaa gagggtgtag
    23701 acacatataa ttacaaagtg tatcaaatcg aaaaaaacga taaatacacc gggtatgaac
    23761 ctttcaaatt aaacttacct caattagaca tcaatttaaa atccgaaagt gtagtaaatg
    23821 aaaatgttaa aaacaacacg atttcaattg ataagttgag tttttcagaa agttcatcaa
    23881 atttatttaa ccctagtgat gttacctctg gcgtttatgt taatcctaca acaggtgcgc
    23941 taagtacaaa tgcgaattac gtatcaagta atttcatcga tattacaggt gctacatctc
    24001 tatctaaaaa taatacaaac aacttatacg ctttttatga tacaaacaaa aactttatca
    24061 aaactagcac gacatcaaca aatacaattt cagttcctag taacgctatt tttataagaa
    24121 tgtctgcaac aactagcgca accaacggaa caatgattgt aaaaggcgac agtctgccat
    24181 cgaattttat agagtataaa cgctatatac ctagcaaata tttagaggta tcaagttttt
    24241 cagctaataa agataaagaa tatccagaca gttacggtaa atgtaactta caaacatata
    24301 ctgctgaaat aagtaaactt ttcgacccca atgcaaatac taggactgaa atagctatca
    24361 ttggcgatag ttgggtacaa ggtggcgaat tcaaagcagg agatcgtttg acattaccat
    24421 taagagacag aatgacaaag ttgtttggcg atggtggcat aggatttgtc ggattagcta
    24481 ataatcatgt tgggaatggt tctgtaactg ttagtacaac aggaaactgg acgcagtacg
    24541 atgagggatt aggtaatatt tcgcagtcta aaggaataga cagcgctatg attgaaagta
    24601 gcacaaaagg cgacagcatc aaagttactt tcgccgaaga cgttgactat tacgaagttc
    24661 atacacttaa cacagggcaa tggcgctaca acatcgacgg taatgaatgg gtaaatattg
    24721 atgcaacatc acaagaagtc acaccaatta caaccaactt aggcaaacac acaatcaaca
    24781 tcgaaatcgt aagtggaaca gtaacattcg taggctcata tgcatataaa ggtaataaag
    24841 gtgtggttat ccataaagtt ggaaatggtg gtttgagagc ttctcatgtc gctagtacag
    24901 atagggataa ctacattaaa caattgaaac gttgtaaagc taatactttt ggcattttgc
    24961 ttggaacaaa tgacatggtc ggcaatgttc caatttcaga ttatgaaagg gatatgaaag
    25021 aaatcatttc aaggattaaa caagcgaaac caaatgctag tatcttctta attgcaccaa
    25081 gtg
//
LOCUS       DAPIDM010000026        24308 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000026 DAPIDM010000000
VERSION     DAPIDM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.026"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(227..532)
                     /locus_tag="RJE42_000948"
     CDS             complement(227..532)
                     /locus_tag="RJE42_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876390.1"
                     /translation="MKGNIQCPRLIKGSLLDFVLETVDVFVNGSKNDCELVNFNYKEG
                     KVVSRYSTKFESNPKLRKKQLISTVKVVRYVIFDFEKILWGIGKVLLKFIILRQCLL"
     gene            complement(778..1257)
                     /gene="rlmH"
                     /locus_tag="RJE42_000949"
     CDS             complement(778..1257)
                     /gene="rlmH"
                     /locus_tag="RJE42_000949"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV5876391.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1623..3944)
                     /gene="adsA"
                     /locus_tag="RJE42_000950"
     CDS             complement(1623..3944)
                     /gene="adsA"
                     /locus_tag="RJE42_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDV5876392.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSSAAEQHTPMKAHAVTVIDK
                     ATTDKQQVTPTKEAAAHQSGEEAATNVSASVQGTADDTNNKVTSNAPSNKPSTAVSTT
                     VNETRDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHG
                     RLAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYD
                     AMAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKKGIRYGIIGV
                     TTPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRG
                     DYLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNDDALAQTGTALANIGKITFNYRN
                     GEVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDD
                     VRTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPF
                     GNTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPS
                     GKRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVL
                     ASYLKTANLAKYDTTKPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDAQPIGKDKVMDP
                     AKQPAPSKVVLLPTNRGTVSSGREGSDRALEGTAVSSKSGKQLASMSAPKGSTHEKQL
                     PKTGTDQSSSPAAMFVLVVGIGLIATVRRRKAS"
     gene            complement(4171..4971)
                     /locus_tag="RJE42_000951"
     CDS             complement(4171..4971)
                     /locus_tag="RJE42_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV5876393.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIKDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5359..6147)
                     /locus_tag="RJE42_000952"
     CDS             complement(5359..6147)
                     /locus_tag="RJE42_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDV5876394.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIQLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6148..7482)
                     /locus_tag="RJE42_000953"
     CDS             complement(6148..7482)
                     /locus_tag="RJE42_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876395.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAIVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGANQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNPDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(7475..9301)
                     /gene="walK"
                     /locus_tag="RJE42_000954"
     CDS             complement(7475..9301)
                     /gene="walK"
                     /locus_tag="RJE42_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDV5876396.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(9314..10015)
                     /locus_tag="RJE42_000955"
     CDS             complement(9314..10015)
                     /locus_tag="RJE42_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5876397.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(10755..10827)
                     /locus_tag="RJE42_000956"
     tRNA            complement(10755..10827)
                     /locus_tag="RJE42_000956"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10792..10794),aa:Asp,seq:gtc)
     gene            complement(10835..10909)
                     /locus_tag="RJE42_000957"
     tRNA            complement(10835..10909)
                     /locus_tag="RJE42_000957"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10874..10876),aa:Glu,seq:ttc)
     gene            complement(11339..12622)
                     /locus_tag="RJE42_000958"
     CDS             complement(11339..12622)
                     /locus_tag="RJE42_000958"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV5876398.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(12901..14301)
                     /gene="dnaB"
                     /locus_tag="RJE42_000959"
     CDS             complement(12901..14301)
                     /gene="dnaB"
                     /locus_tag="RJE42_000959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV5876399.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSMEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(14333..14779)
                     /gene="rplI"
                     /locus_tag="RJE42_000960"
     CDS             complement(14333..14779)
                     /gene="rplI"
                     /locus_tag="RJE42_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV5876400.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(14776..16743)
                     /gene="gdpP"
                     /locus_tag="RJE42_000961"
     CDS             complement(14776..16743)
                     /gene="gdpP"
                     /locus_tag="RJE42_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDV5876401.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(16758..17696)
                     /locus_tag="RJE42_000962"
     CDS             complement(16758..17696)
                     /locus_tag="RJE42_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDV5876402.1"
                     /translation="MGVAYVFSKIQPKATIMATIALVIVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIVSLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(17989..18957)
                     /locus_tag="RJE42_000963"
     CDS             complement(17989..18957)
                     /locus_tag="RJE42_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDV5876403.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQTIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKERFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(19017..19115)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(19320..19649)
                     /locus_tag="RJE42_000964"
     CDS             complement(19320..19649)
                     /locus_tag="RJE42_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDV5876404.1"
                     /translation="MITHMNILILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(19646..20341)
                     /locus_tag="RJE42_000965"
     CDS             complement(19646..20341)
                     /locus_tag="RJE42_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDV5876405.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAISDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSKFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(20988..22274)
                     /gene="serS"
                     /locus_tag="RJE42_000966"
     CDS             complement(20988..22274)
                     /gene="serS"
                     /locus_tag="RJE42_000966"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV5876406.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(22364..22579)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22652..24166)
                     /gene="hutH"
                     /locus_tag="RJE42_000967"
     CDS             complement(22652..24166)
                     /gene="hutH"
                     /locus_tag="RJE42_000967"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDV5876407.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVEG
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLHRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         7223 a         4808 c         3635 g         8642 t
ORIGIN      
        1 attcagaaaa ttgattaatg tgtttcaata aaatctctcc ttctttgtag acatattcat
       61 ttttatacta attaatatca ttcccaaaaa aatttctata caaaagagaa aaatattatt
      121 tatcgtttaa cttaaatctt caatatatag gtgggcctat gtatcatttt gtgccaattt
      181 gaaaaaaacg ggaatccagt ctgtttctga gtgtatttca actttcttat agtaaacatt
      241 gccttaatat gatgaacttc aataaaactt tccctatgcc ccataaaatt ttctcaaaat
      301 caaaaataac ataccttaca acttttaccg tcgatatcaa ttgctttttt cttaatttag
      361 gattgctttc aaattttgta ctataacgtg aaactacttt tccttcttta taattaaaat
      421 ttactaattc gcaatcattt ttacttccat ttacaaaaac atctactgtt tctaacacaa
      481 aatctaataa acttcctttt attaatcgtg ggcattgtat atttcctttc attctttctt
      541 gattccatta gtttaaattt aaaatttcat catcaatttc ttaattgaat tgtagttcca
      601 taatcaatat aatttgtaca gttattatat attctagatc atcaatagtt gaaaaatggt
      661 ttattaaaca ctctataaac atcgtatgat attgcaaggt atactccaat atttcatata
      721 tgtaattcct ccacatctca ttaaattttt aaattataca caatctaatt tttagtttta
      781 tttatgataa gcctctccac gcataatctt aaatgctcta tacacttgct caattaacac
      841 aacccgcatc atttggtgtg gaaatgtcat tttgctgaat gatagtgcgt agttactgcg
      901 ttgtaagaca tccttgtgca ggccgtttga tccgccaatg acgaatacaa agtcgctttg
      961 cccttgggtc atgcgttggt tcaactcttg ggccaatcct tcggaagata gcatctttcc
     1021 ttgtatttct aatgtaatga ctgtggattg tggtttgatt ttggctagta ttcgttggcc
     1081 ttctttttct tttacttgct caatttcttt gtcgctcata ttttctggtg ctttttcgtc
     1141 tggaacttct atgatgtcta tcttggtgta tgggcctaaa cgtttttcat attctgctat
     1201 ggcttgcttc caatatttct cttttagttt ccctacagct aaaatggtga ttttcatgtc
     1261 gtttggtcct ccaaattgtt atcaactttc cagttatcca caagttatta acttgttcac
     1321 actgttccct cttattatac caatattttt tgcagttttt gatattttcc tgacatttat
     1381 gcccgttttt tagtgtataa cctgtgtatt tgtggataac tctgtgggca ttgtgggtat
     1441 ttctctttaa attgtacaca attctggata actatcccca tttctgtgga taaatacctt
     1501 gatgtcttat tacttatccc cgtttttata tcccatattt atcaacggtt atcgcttttt
     1561 ccataaaaaa cactaccgta cctcttattt aagaaataca gtagtatggt tttcaatata
     1621 ttttagctag cttttctacg tcgtacagtc gcgattaaac ctatacctac tactaataca
     1681 aacatcgctg ctgggcttga actttgatca gttccagttt ttggtaactg tttctcatgt
     1741 gtgctacctt taggcgctga catgctagcc aattgtttcc cactcttact tgatacagca
     1801 gttccttcca atgcacgatc agaaccttct ctaccactac taacagttcc tctattcgtt
     1861 ggcaacaaca caactttact tggcgctggt tgtttcgctg gatccatcac tttgtcttta
     1921 ccaattggtt gtgcatcttt accagactta ctacctttgc taccttgttg tcctttagct
     1981 ggttgttcac ttactgctgg tttacctaat aacatacgtt gtggttttgt cgtatcatac
     2041 ttagctaagt tagctgtttt taaataactt gctagtactt gatctaatga aataccttct
     2101 tctctagggc caccgaacat actatatccg tcgccacctg atgctgtgaa gtcattcatc
     2161 gttacgtgat atacacgttt taaatcaata ttttcaaact tacctgtctc tttatttaaa
     2221 atttgaatag cgttaattcg tttgccagat ggtttattca tatcatagta aacacgaatt
     2281 gaatcagaga tatgtagtaa accaccattc gctgttaata ctgtcttacc atctttttgt
     2341 gttgttggtg cgcctaaact atgttcaaaa gctgtccaga cgtctgaacc ttttacatca
     2401 atttgcgcaa tcgtatttcc aaatggtaat actgagatta aatcatagcg tgtcacctta
     2461 ccttttgcga tagaggcacg aatacctcca ccatttgtca cggcaaagtc agtcttttta
     2521 gagaaattct taacgccata cgcttccata gcatctgcaa ttgcgtttcc taaatttgtt
     2581 tcacgcgttc taacgtcatc tctttctcct ttgaaatcga tcgtattatt tggaataata
     2641 acctctgctg tttgtgctct aaatgtttga tcagcttgat taatttgttc agctaatgct
     2701 ttgttcggtg ttacattttc aacgtcttta acattaatca atgatggttt aatatttgat
     2761 acctctccat tgcggtaatt aaatgtaatc ttaccgatat tcgcaagtgc tgtacctgtt
     2821 tgtgctaatg catcatcgtt ataaatttga ccattttgaa gtacggtatg tgaatgacca
     2881 tcaatgactg taatacgttt tttcaattgt ggattttgac ttaattgttt cactaagtaa
     2941 tcaccacgcc atgtttcttg tgttgaagga tcaatcccta aatgtgatat aacaacaaat
     3001 gtatctacgt ctttataaat acgcatcatt tctgctgtca cactttgtaa tggatctcta
     3061 aattcaacac ctttaatgcc ttcaggtctt gttttcgtct ttgtttctgg tgtcgttacg
     3121 ccaataattc cataacgaat accctttttc gttacaatcg ttgaaggttt aaatgcgcgt
     3181 tttccatctt tataaacgtt agtacttagc atcgggaagt ctaacatacc ctctaacttt
     3241 ttcaactggt cgtatccaaa gtcaaattcg tggttaccga ctgccatagc atcataacct
     3301 actgcattca ttgctttagc catttcttca cctttagact ggtttgaaag tggtaaacct
     3361 tggaaggcgt ctcctgcgtc taacattaaa tctggctttt cttgttcttt tactgttttt
     3421 aatttagcca taccgatgac acgccctttt tcttcagcta gtcggccatg gatatcattt
     3481 gtatgtaata ttttatgtgt agttgtttgt ggtgcattag cagcaaacat tcgtggtgaa
     3541 agtgatgctg ttttagcatt tgataatgtg aatgtcgctg attgagttgg tttttgtgtt
     3601 gaggcttgtt gtgtatctac gtcgcgcgtt tcgtttactg ttgttgaaac tgctgtagat
     3661 ggtttgttag atggtgcgtt ggatgttact ttgttgtttg tatcatcagc tgttccctgt
     3721 actgatgctg atacgttggt tgccgcttct tcaccagatt gatgagccgc tgcttccttt
     3781 gttggcgtta cttgttgctt atctgttgtt gctttgtcta tcactgttac tgcatgtgct
     3841 ttcattggtg tatgctgctc agccgcgctc gagacttgcc ataatcccat tgcactaaaa
     3901 agcaaaacga gccatacact tgttttaagt aataaagctt tcatttcgtt ccctccatta
     3961 actgtcatac accaatatat tatcataatc cttatattaa aatgattgat tttatgtaaa
     4021 ttatttataa attacattta atatcgaatt attttttaca tttcaactag aaccttaatc
     4081 tcaacaacag ccatttccca ccaacaacat aaacaaaaac ccgataaagt cgtttcacag
     4141 cagtgcggaa ctttatcggg tgactctcat ttatattgta tatattggcg ttggaatagc
     4201 tttatccgta tcacatagca atacttcttt ttccgtatca atatcgtgtt cgttcaatac
     4261 ttggccaaca ctcatacgcg ccaaatcttt catgttatta tcttgtgata aatgcgataa
     4321 gtaaatacgt ttcgtgttac ctgtaatcac gtctgtcatc gcatgacccg catcctcatt
     4381 agatacatga cccatatcgc ctaaaatacg ttgtttcgtc ttccatggat aacgacacat
     4441 tctcaacata tcgacgtcat gattactctc aaaaataaac gcatcgctgc cacgtatcat
     4501 acctttcata cgatcagaca cgtaacccgt atccgttaaa atcgtaaact tcttatagtt
     4561 attatggaaa atataaaatt gcggatctat cgcatcatgt gacacgttaa acgattcaac
     4621 atcgaaacct gcaatagatt tcgtttcata aggattaaaa ataaatttct gatccatagg
     4681 aatgcgacta tctttctttt caattgcctg ccaagtcttt tcattcgcat aaattggcaa
     4741 ttgatattta cgcgccaaaa cacctaatcc tttaatatga tcaatatgtt catgggttac
     4801 taaaatacca tttaaatctt taatattacg gtcaatttga ctaaacaatt cttccatttt
     4861 cttgccagtc aaaccaacat caactaatag actacctttt tcattttcta caaaagtggc
     4921 gttacctgta ctaccacttg ctaatacact catgcgtatc aagcggctca tcctttctat
     4981 ttatgcagtc actctctcta aatgactgca atatacatca gtgcaaatat acgatttccc
     5041 ctacagaaac tatcagtata ttagctgttt tgtatatctt tcctattatg taactttagt
     5101 tcatcgaccg gtctcttata attaaatttt gataatcacg tgcaatgaat gtcctaatta
     5161 ttataacaat gattagacaa atactaaagt ttttaaaata gcagttacgt taattttaca
     5221 atgcggttct acttaatata tgaataaatg cgcatgttag gacacaaaaa cgtcagccat
     5281 aataagccat accagcaaaa agctaacacc gcttctcaca tatgatgagc ttgcaatgct
     5341 agcttattac gattcatatt aatgattaat aattttaggg ttattatttg tcgcttcgac
     5401 atagtaagta ttcgttttgt ccttaccgtc atgcttcact ttaatttccc agtttggttg
     5461 taacaattga acattcgttt cgttcacgac tgagtagtaa ccaagtctag cattaatgac
     5521 ttcatcacct tttttcaagt aacgattata atataatgcc tcgattgctt ttctcacacc
     5581 aatcacttga tgcttcttat ctgcaccatc tgtgggctta atgtcatcca tcattgattg
     5641 tttataacta gtcgctttgt tatcttctat attaaagttc aacatcgctt tactattatt
     5701 caaaatcggg aaattatcat acgtttgttc gtattttaca gaaccagaac taatctcact
     5761 taattgatat tctgaacctt tgaacacacg tttgttaaca taatctttca aatcttttaa
     5821 gttattgtca ctgaccttta ccgattgact aatgttcgca ttcaataatt ttccaccgtc
     5881 tgatgtggtc aaatccgaat cacccttagc tttagaacta aagtcttttg aacgccccgt
     5941 aatttgttct aattgtatgc ctttaactga tttatttaaa atactagccg gtactttaat
     6001 ttcttcttgc tgaaaattaa cttcattatt actctcaact tcattaatgt gtgagcgatt
     6061 gactttatta acataaatcg atactaacac gatgttgaca agaataaaca cgaaaatgaa
     6121 aagtgtcttc gtcagtttcc agttcattta ttcaagcctc ccatcgttat aaacatacca
     6181 ttcgccatca tattctacat accagcgtgg tacgagttca ctattaatct gcacttcaat
     6241 gtgattatga tccggattat cttgcatttc gtaaccgata gcgatattcg ttactttttc
     6301 aaaattaata tcactattgt tcgctaagct tgaacgcaca gactctaatt tcggcaacga
     6361 tttattatcc tcactattta acacaacatc ggtgcgcaat aacgaacgac gataatcaaa
     6421 gacgcccttt tcaccccaag tgacttgaat ttgattagcg ccttctttat taaacgttgg
     6481 ataaccatta aggaaacgtt gatatgttaa ctcgcctgac tgattattcg tactaaacaa
     6541 tctgaagtct tcgtttaaga aaccaccatg accattaata aaatcaaagg ttcctgggat
     6601 cgtttcttcc attttgcttg aacttgcttc atcttcggac aagtttttat aatggtattt
     6661 ttcattttta tcgttatagt ttgcgacacc tgtattattg ttgtaggttg taacaccact
     6721 ctttgaacta cgaacgatgg ttgaatcgtc aaatagtata gcattcattt tctcaacact
     6781 aatcgtgtta aataccatgc gatatgtttt taacttctca ggtttacttg gtgcaaaaac
     6841 atgcgtcgta cgatcaattg tatctttgtt tgtgatgata tctgtgtaag gttgcatatc
     6901 ttttttcact attgctaatg catctaaaaa atgatcattt ttcgttgtag ttgtcaattt
     6961 tacgtaatcg tggcgatctt tgcttatagc ataaagcacg atattatcat cagcatcatg
     7021 atctatgacc aaacgattga aattgaaatg atttggtact ttcgcattca tgttcagtac
     7081 ttgaccaaga tatgttgata acggtaaatc atacgtaaaa tcgaataatg taaaatcact
     7141 gctcaaatca ggaatcatca agttatgatc acgacgaaca tgttccacgg acttcacttc
     7201 tttatttttc aaaggtttcg tcagcttatt cacattagat accgtcgcaa tcgtaccttc
     7261 tggatgatca tttttcgaat gaataatctg aaatggcgta atagttgtat ccattttggc
     7321 tgtcataggt gtcgttaaag gtttcgtttc actcttctta ctatctgtat tgtcgacatt
     7381 tgcaatatca ggagaaaagt tccataccat atatgtcaat acgacactca tcaagacgag
     7441 tagcgctaaa atgacagatt taatatgttc cttattattc atcccaatca ccgtcttcaa
     7501 tgacttcaca tggaagtgtg ataaagatag atgtaccttg accttctaca ctgtttgccc
     7561 atatacgacc attgtgcgct tccacaatct ctttcgaaat ggctagtcct aatccagtac
     7621 cacccatttt acgcgtacgt gccttatcta cacgatagaa tcggtcgaat atcttatcga
     7681 ctttattgat aggaataccg atgccattat ctttaatacg aatcgtcatt cgattataaa
     7741 gtggattttg tttcacgtgg aactcgacac gtttatcgcc tctagaatat ttcatcgcat
     7801 ttgtaatgac attatcaaat acttgcgtca ttttatcagg atcaaattct gtgaaaatcg
     7861 tctttttcgg aatatctcga ataaatgttg tatctttcgc agacatttca tgtcgattaa
     7921 taattttatt aatgaacatg ttaaagtcga taatttcttt gttgatttga tcagactcat
     7981 tatccatttt agataactgt agcaagtcat tgaccagtcg aatcattcgt tctgtttctt
     8041 cacgggtaac agataaaaat tgtggcgcaa gttcctcatc tttccatgca ccttcttcaa
     8101 gtgcttcaat gtaactattc atagaagtta aaggtgtacg taactcatgt gatacattgg
     8161 caacaaattc acgacgctca cgttcaactt gttgttgttc agtaacgtca tgtaacacag
     8221 cgatataacc agttacaaat cctgtttcct gcacaatcgt actaaagtta acacgtgcga
     8281 ttagaccttc ttcttcattt aaatctaata agaaactatc attattctct tgaatttctt
     8341 caagtttaaa ttcatcttca agacttaata cacttaacat gtaataaccg atgatgtctt
     8401 ctttcgccat accaagcatc ttaagtgcca tatcattgac gatacgaata cgtccacggc
     8461 ggtctgttgc gataatacca tcactcatat gggtgataac tgagtccagt ctacgtttct
     8521 cactttcagt attagcctgc gcttcttgta cacgtttaga caagttatta aatgctaaag
     8581 ctaattcgcc aatttcatca ttaccataaa tcttcacacg ttgcgtatag ttacctctgg
     8641 acatttcaac cgtctggtta cgcatatcgg tgattggttt ggtaatcgtt cgcgctataa
     8701 agaatcctag gatgactgtg attaataatg aaatagctgt accaacaatg aatatttgat
     8761 ttatattatt taattggtta taaacgtcat taatttttga ttcgatataa atattaccaa
     8821 ttaccttttt atcgacttta actgggatat tatataccca gacacggtcc ttaccaccgc
     8881 cataatcttt taaaattaaa tgatcgtttg attgtcctag tgatagtgct ttttggacag
     8941 aactatcatt cgctttttga ttgattagac tacggttaga ctgcttcgtc gtcgcaataa
     9001 taatttggtc tttatctata aaacgaattt ctccaatttc ttgacggttg gcatactcac
     9061 ttaataaatt ttgaatatct ttttgtgcat ttacggagcc cttttcgtca tatacttttt
     9121 caatactaat ttctaattgt ttagcgtact gcgtaatatt cttcttaaaa ttatcaagca
     9181 gctctttttc aaggttattt gtaaaataca gcccgataat ttgcatacca atgataatca
     9241 gtaatacata aacaattaca agtttagtat gaagggattg tagttgtttt agccacttca
     9301 ttcgtttcga cctctactca tgttgttgga ggaaatatcc aacgcctcta cgcgtcacaa
     9361 tgtattcagg atgtgacgga tcatcttcaa tcttttcacg taaacgacga atcgttacat
     9421 cgaccgtacg tacatcgcca aagtaatcat agccccatac tgtttgtaat aaatgttcac
     9481 gtgtcattac ttgtcccata tgttttgata aataatggaa caattcaaat tcacgatgtg
     9541 ttaattcaat atcttcgcca cgctttttaa tagaatatgc gtctggataa atcacaatat
     9601 ctttaattgt gatttcattc gttacatttc cagtgtcttg tgctggttgt gagtaatgac
     9661 gacgtaagtt cgctttcaca cgtgcgatta attcacgcgt actaaacggt ttcgttacat
     9721 agtcatctgc acctagttct aaaccaagca ctttatcaat ttctgaatct ttagcagtaa
     9781 gcattataat tggcatttcg tattttttgc gcacttcacg acatacttcc ataccatcac
     9841 gaccaggtaa catgatatct agcaatacga tgtctggttc ttcttcataa attaagtcga
     9901 ctgcatcatt accatcgtat gcacagtaca catcgtatcc ttcttttttt aaattaaatt
     9961 ctaaaatatc agcaatcggt ttttcatcat caactacaac aacttttcta gccatttgca
    10021 taaacctctt ttcttaaatc attaatatta ttttatcatt ttttgttctt tatcgtttat
    10081 gtttgtgtaa aaaatcacag gatgtcgctt actatttttg ttattgtgcg cattttccgt
    10141 cttataacgt atagtaacct attatataat ttaccattta taagctatta attctatttt
    10201 ccaatataaa cttatatatt aatatttttt attagtttct cacggcggat tgtgataatg
    10261 atatttaaca caatgatgag gtctctattc caataataaa atcagggtaa tggcaacttc
    10321 tagttgcacg tcattgctca gaaaataaat atcattattc gttactttaa caattgatgt
    10381 tatggaggat tggtggcata tcacaacgaa gaaatatgtt gacagtagca tatatttgta
    10441 cgtctaataa agattttatt gtctacgttt atctttcata tccaaccact aagttacttg
    10501 aggttcatac attataaaga aaatgggagt gggacagaaa tgatattttc gcaaaattta
    10561 tttcgtcgtc ccaccccggc aaggttgact agaattgaaa aaagcttgtt acaagcgcat
    10621 tttcgttcag tcaactactg ccaatataac ttcgtagagc atagaacatt gatttatgtc
    10681 ccagcctgat atgtctttta ttaatataat attaaacgca aaaaaatgcc tatcctcttg
    10741 gataggcatt tgtacggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
    10801 gcgtgttaac cgctacacta cgagacctaa aaaatggtga ctcctacggg actcgaaccc
    10861 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caaggagccg ttcttaagca
    10921 caagatagat tatagcacag ccaatcttgc catgacaaga tattttttac ataattttgc
    10981 tattattttt tgaagaatgt tataagtgaa aaattgcatt ttattggctg tctattcatt
    11041 ataataatta cgctattttt gaaatttttc tattttagat actttcattc acacttacgt
    11101 ctactctatt ttaatagcta ttttcgacat cagacttctt acattcaagt atttcatctt
    11161 aattttcgtt aaatatcatc taatcttcta aattatgact agcaaatatc acctttacgt
    11221 tagtattttt tatctaaata atcgaatcga tacagaggca aacatagcaa aagcctccta
    11281 ccaataaaga tagaaggctc atgtatttat aatcattgtg tatgacttat ataaagttct
    11341 accacaattc ttttaatagg tttgtttgtt ctctatctgg accaactgag aagatagaaa
    11401 tttgtacatt acataattct gaaatacgct ctaaatattt acgtgcattt tcaggtaatt
    11461 cttctaaagt acgcacactt gttacgtctt ctgtccaacc tggtaactct tcaaagattg
    11521 gtttacaacg ttttaattga tctaagtttg ctgggtactc agtaatttct ttaccgtcta
    11581 attcataagc tgtacaaatt ttcactgtgt ctaggcctgt taaaacatcg attgagttaa
    11641 tagataaatc tgtaatacca cttacacgac gagagtgacg taatacaact gaatcaaacc
    11701 aacctacacg acgtggacgt cctgttgttg taccgtattc acgaccaact tctctaatat
    11761 gatgtccatc ttcatcgaat aattcagtag ggaatggacc atcaccaaca cgtgatgtat
    11821 aagctttaca tacaccaatt acctttgaaa cgaatgtagg acctacacct gtaccaacag
    11881 taacgttacc tgcaattgga ttacttgatg taacgaatgg atatgtacca tggtcgatat
    11941 ctaacattac accttgcgca ccttcgaaaa gtaccttttc atctgctaca aatgcatcgt
    12001 ctaagatttt tgatgtgtct gttacaaatt cttttaaacg ttgacctgca gcatagtatt
    12061 cttcaaagat atcatcaaat gatggacatg tttcgttaaa catacctttg aaatatgctt
    12121 gtttatattc aatgtttgat tttaataatc tttcgaatgt ttctttttca agtaaatctg
    12181 ccatacgaat accgatacgt tgaactttat ctacatatgc tggaccgata ccttttttag
    12241 ttgtaccaat cttattatca ccgcgtaaac gttcttcata ttcatcttgt gctaagtgat
    12301 atggtaaaat cacttgcgca cgattagata tacgtaaatt acttgtagga atgccacgtt
    12361 catttaatcc gtctaattct ttcaatagtg caactggatc aacaacgaca ccgttaccga
    12421 ttaccgctaa tttgtctttg taaaagatac cagatggtac taaatgtaat ttatatgttt
    12481 ctccgccaaa ttgaatggta tggcctgcat tattaccacc tgaaaaacgc gcgataacat
    12541 ctgactgttc tgccaagaaa tccgttattt ttccttttcc ttcgtctccc cattgtgtcc
    12601 caactactac gattgatgac atgtgagcac ctccaagttt ttctcattta accatttgat
    12661 attctaccaa tatcgagacc ataatgcaaa taaaaatcga acattgattt tcggcctttt
    12721 taaaagaaaa atcattttag caaatagtct tcaacgtcct aaaaaccctt taacagaggc
    12781 gttgacagta ctaatttatc acttacaata cacaaactta acatatatca acccgttcag
    12841 aataaaataa aaaccgtaca atttcataat cataatgatt attgtacgga aaaacttttt
    12901 ttacatcata tcagcatgtg cataatcgat atctgtaaat ttattatatt gtttcataaa
    12961 gtgtaactta actgtgcctg ttggaccatt acgttgctta gcaatgatga tttcaatttc
    13021 accgttttca tcattcgttt gtggctcaaa accaccatca tcgtcatcat cttcatcgcc
    13081 gccacggtta tagtaatcat cacggtataa gaatgcaacg atatcggcat cttgctcaat
    13141 cgaaccagat tcacgaatat cactcatcat tggacgttta tcttgtcgtt gttcaacacc
    13201 acgagataac tgacttaatg cgataactgg acattctaat tcacgggcta atgcttttaa
    13261 tgtacgagag atttcagaaa cttcctgttg tctgttatcg gacgcacgcg aaccactacc
    13321 ttgaatcaac tgtaagtagt caatcacaat catgtctaag ccatgttctt gctttaatcg
    13381 acgacattta gaacgtagat cattaattcg aatacccggt gtatcatcaa taaagatttt
    13441 cgtacgtgat aatttaccta ccgctatagt aaaacgactc caatcttcct cagtcatagt
    13501 acccgttctt aagcggtttg agtcaacatt accagaacta caaatcatac gtgtggctaa
    13561 ctgatcagca cccatctcta gtgagaaaat accaactgta tacatatctt catgcgttgc
    13621 aactttttgt gcaatattaa gtgcgaacgc agtcttacct acagatggac gcgctgcaag
    13681 gataattaaa tcatttcggt tgaaccctgc tgtcatttgg tctaaatctc gatatcctgt
    13741 aggaatacct ggtgtttgac cactattttg atcaagctct tcagctgttt catatacttg
    13801 tcctaagacg tctcgaatgt ctttaaagcc atcgctttca cgagaagatg atagctctaa
    13861 aattcgacgt tctgcatcac ttaaaatcgc atctagttca agttcatcat tatatccatc
    13921 attggcaata ctatctgcag tttgaatcaa tctacgtttt aatgcatgct tagaaacgat
    13981 atcagtataa tactgaacat tgcgtgtcgt tggtacattt gtagataact ctgcaagata
    14041 ttgcgggcca cccgcttcat tcaacgtacc ttccatcgat aattgatcca tcaatgttac
    14101 aacatcaatt tctttattat cttcatttaa gtgcatcatt gcacggaaaa tatgttgatg
    14161 ggcaccccta taaaacgact caggaagcaa cacttcctga gtagtattaa tcaattctgg
    14221 atctataata attgaaccta agacagactg ttcagcttca ttgttatgtg gcatttgatt
    14281 ttgctcatac attctatcca tgaatggtta cacctcttat ttcaatccaa ctttattgtt
    14341 caactgtgtg tacgcgaatt gtaccttcaa cttctttatc taatttaaca ggtacattcg
    14401 tatatcctag ggaatgaatt ccgtttggta aatccatttt acgtttatca attttaatat
    14461 catgttgtgc ttttagtgct tcggcaattt gttttgtact tactgatcca aacaatttac
    14521 caccttcacc agtttttgct gatacttcaa cttcaatgtt tgataaagtt tcttttaatg
    14581 ctttagcatc ttcaatttct tgttggcgtt cttgttttgc acgttttttc tgtaactcta
    14641 attgtttaag gttacctggt gttgcttcta cagcataatt ctttttcaat aagaagttat
    14701 ttgcataacc tactggtact tctttaactt cacctttttt acctttacct ttaacatctt
    14761 gtgtaaaaat tactttcatg catcttcact cctacttaat tgttctgtaa ttgcttgttg
    14821 taattgtgct atcgcctctt cgactgtcac acctttaagt tgtgttgcag cattggttaa
    14881 atgtccaccg ccaccaagtg cttccattgt taactggaca tttactgaac cgagtgaacg
    14941 cgcagatata ccaatcagat tatcttcacg tctcgcaaca acatatgatg cttcaatacc
    15001 ttctaaactt aacagttcat ctgctgcttg tgcaactgtt actggatgat aaattttatc
    15061 gtctgaacca tgcgcaatgg ctatgccatt atcttcgact tttacagttc gaattaattc
    15121 agatcgatta atgtaagtat ccacatcatc ttttaagaaa tgttgcgtta aaatcgtatc
    15181 tgcaccatgt gcacgtaaat aactcgctgc atcgaatgtt cttgatcctg ttcgtaatgt
    15241 aaagtttctt gtatctacaa taatacctgc atacatcact gttgattcaa gacgtgttaa
    15301 acgttgttct gttggttgat attccagtaa ttcagttaca agttctgctg ttgaacttgc
    15361 gtatggttcc atatatatca acaacggatt agagatgaag ctttcaccac gtctatgatg
    15421 atcgataaca actttacggt ttgctttatt taagacattt tcatctaaaa ccagttccgg
    15481 tttatgcgta tcaacaatca ctacggttgt cttagatgtc atcatatccc aagcatcatc
    15541 tgatgtaata aatcgctctc ttaactctgg ctttttatca atttcgttca tcacgcgtcg
    15601 taatgttgga tcaatgtcag tctcatttaa tacgatgtat gcttctaaat tattcatcat
    15661 tgcaaatcta gacacaccga ttgctgcacc aattgcatct aagtcaggac gtttatgtcc
    15721 catgataatg actttgtcac cctctgcaag gatatctttt aacgcatgtg agataacacg
    15781 tgctcttaca cgagtacgtt tctccatcgg gtcagtctta ccgccataga aacgcacatt
    15841 accattaata cttttaattg caacttggtc gccaccgcgt cctaatgcta agtctaggcc
    15901 tgattgtgat aattcaccta aatcgattaa attttcagta ccttcaccaa caccaatact
    15961 taatgttaat tgggcacgat aaccaacact tttttcacgt aattgactca agatatcaaa
    16021 tttagattct tctaagtcag ctaatatttt ttgatttaaa taggctacga attgatcgga
    16081 actatacctt ttaaagaata tattatactc agttgcccat cgactaataa cacgcgttac
    16141 cattgaattg atttccgaac gctgcgtatc attcatattt tgagtaatct catcgtagtt
    16201 atctaaaaat aatgtcgcaa tgattggttt agaattttca tatagttcat ttgtttgtac
    16261 ttgttcagtt atatcaaaga aatagaggca gtgatcattc tcagaataac gtacttggaa
    16321 atgatactga ttatattcta tttcaacgga tttcactcta tctaattgct ttaaaatgtt
    16381 tggaaatact tcatttacag attcagaaat gacatttgct tccatatgat ctgtcataaa
    16441 ttggttaacc cattcgatgt gatcattttc atctaaaaca atgataccaa ttggtaaatg
    16501 tttgattgct ttattatttg ttgttgaaat ttgagcactc aaaccatcta cataactatc
    16561 cattttcatt aaagcttgtc tgaataaaac gatgctaaca ataatcatca cgacaagaac
    16621 gatagatgca attagagcta taagactatt aaagataaac catacaccca ttaaaacaat
    16681 tgctgtgatg atcatgatga caaatggtat tagtaaagct ttcttagtgg actgccgatt
    16741 cattattcca cctctattca ctttttagaa ttatttttca tgattcgctt caaattcaaa
    16801 cttaaatcga taacaccaag tagtcctaca atatgcgtcg taggtgtcag tattgtaccg
    16861 ataatcaata gtaaaatcgt tactgcattc ggcaaacctt tcgctttacc aaagaaatga
    16921 ataacactta acccttgaat atacatcact aatgataaca caagttggaa gtttaaaaga
    16981 atgctctgga acacactcgg ttgacctgta aataataaac atatgataac aataatgtat
    17041 atccataata aaataccgct catttgccac gcgaaaagtg gcttaaatac aggtgtagcg
    17101 attttaaatt ttcgtaaaat cggaaatgtg acgattaagt taattaagac gattaaaaat
    17161 gtaatgataa tgatgaaacc tggtaattga acggtcgctt gtctaaaccc ttcttctaat
    17221 atttgggtca tattcgcatc ggcaccgctc atcgtaatcg cttcatgtaa tgtttgcttg
    17281 aaaggtttta ctatgctcgc tgatggtgga attcttccga atgtttgtag taacataaag
    17341 gcgattaatg agattaaact catcgctacg gttgttacgt acaatattct ttctttagac
    17401 gttctttctt tgagcaattg accaataatt aaacttacaa ttaagactaa tatgatagca
    17461 cttaaaacga aagtattacc taaaacagtt gttataatta ctgtaataag tgcactaatc
    17521 ccgaaagatt gtattgattt attccataaa acgatacctg gtatggttgc aaataatgca
    17581 aatattagtc ctaaaccagg cactagatat aaagctaaag cgacaatgac caacgcaatc
    17641 gttgccatta ttgttgcttt aggttgtatt tttgaaaaca cataagccac tcccatattt
    17701 ttaactatag ctattatttt aacctcttta atggaattta acaatttata gattgtatgc
    17761 ttctatttca tttaattgaa taataacttt catgtttaat aagtaattaa catactcatt
    17821 tgtatcactt ttgtgtgctt tcattttcaa catgattatt taatcccact acatagcaat
    17881 caagcttgat ttagatttac aattcatttc cactctcatg tactctagat gtttttgaat
    17941 atgacaactg tgatttagtg gcttcattct ttgaaaatat atacattatt acttgcgctt
    18001 aaaatgcttt aaatttaaga aatgatataa gttaggtgcc caggtactaa agtttagtag
    18061 gaatccatca tgccctacat tatcaggcac gaagaaatga cgatgatatt taaaacgttc
    18121 acctaatgca cgaacttgat catccggata taacaaatca tctataaagc ccattgttaa
    18181 gactttcgtt tctaaatttt taaaaacatg cgttacgtct gtgcgacctc ggtcaatgtt
    18241 gtgactatcc aatacatcta gcagtgtcag ataacaattc aaatcaaatc gttctttaaa
    18301 tttattacct tgatgttgtt ggtatgcgac tacttcatcc ggcgtgaaac gttcatcata
    18361 actttttgat gatcgatatg tcaaaaaacc taattggcgt gcaatactta gcccttcctt
    18421 accaccaaga tgaatggtct gacgcgcaat ttcattgaaa gctctactat aagatgatgt
    18481 tcggcttgtt gcagcaagaa taatggcttt atctacttca aactgttgat tgtaaagtag
    18541 ttccatcgct tgcatacctc caagacttcc ccctattaaa atattaattt tgtcataacc
    18601 aagcgcttgt atacctcgtt cattcgctct aacaatatct cttaatgtta attttttagg
    18661 aaaatgaggg tcgtttaaag gtgaacttga accgaaagga ctaccaataa catcaaatgt
    18721 taaaaattga taatcgtgaa tgggtatata tcccccatca ataatttctc gccaccaacc
    18781 cggataatca tctgttccat atgttaaatg attgccagtt aatgcatgac aaactacaac
    18841 taatggttgt ccatgataac cgacatgctc atatctcaaa cgcaagttat ctatgacttc
    18901 cccagattct gtaataaatt tccctagatt taaagtatct actgtgtaat ttgtcattgt
    18961 tctttcctcc ttaaacaaaa aaacttctca ccctattgaa aagtaagaag tctttatact
    19021 tatcattcga gtaactcgtt ggttttagca ccgtgctata aagtcggttg ctgaagtatc
    19081 acagggccaa gtccctcaac ttctcacaat aagaaatatg aaattgttat gtgttagttg
    19141 agattcagtg atgaattact tttatcattt aaaatgttgt tatcattgtc atgcgttagc
    19201 caaatcgctt acgtatacac gattcccaat cttaacatag acgatttgta tatcagaatt
    19261 ttctgattac taacagttta cctaagttta aatatctgtt caatgatttt cagttatttt
    19321 taaaagaaaa atcgtaatgc tgccatgata acaatcccac taataattgt aatagttaaa
    19381 ctacgcgtga ttatagataa aataaccgtc ggaatgagcg cgataatgta agggatgttt
    19441 aatgtatacc cctcaccatg aggcgtctgc tgaataatgc tgtcaatgac aagtgccgta
    19501 aatagtgtga tcggaataaa tgatagccat cgaaccacga catcaggcaa ttgtactttt
    19561 gaaatcatga taaaaggtat aattcgaatt aatagcgtta cgataccaca caataaaata
    19621 agtattaaga tattcatatg agttatcatc gttccatcat cactcctaac gctgctgaaa
    19681 ttgtggctgc aattaatatt gctagatatg aaggcataaa cttacttaac gataacatca
    19741 ttactatgac ggcaataatg agtactatgt aaattcttaa acgcgattta gtaattgatt
    19801 caaattgtgc aatggctaaa aagataaaca tagccgtgat agcaaaatct aatcctaatg
    19861 cttgtggatt cgaaatatac tcgccaaaca atgcacctgc tacacatgaa attgcccaaa
    19921 ataaatatgc tgtaatatta agaccatgca tccaacgatc actgatagct tctcctttta
    19981 aataaggtgt aatagcaaca ccaaacgttt cgtcagtgac taatgaacct aatccaacac
    20041 ggttccaaaa cccatatgtc ttgaagtttg gtgcaagcga catacttaaa aggaacattc
    20101 ttgaatttac gataaataca gttagtacaa tcgctgatat cggtgtacct gctataaaca
    20161 acgcgcacat aataaattgc gcagcaccgg catatataac aagacataac aagacaattt
    20221 ctaaaatact aaagttttga gacgaagcca caataccaaa tgaaatacca acaccggcat
    20281 aacccaataa tgttgggata cactcttgca cgccttgtct aaaacttaaa tgtgttgtca
    20341 tctcaattac ctcctttgcc actttatatt tatataatta cgaaaaaata atatacctag
    20401 tctaatatag ttctatatca caagcaataa cgttagactt cggtttgtct gaggttagac
    20461 aaaccgaagt attgaaatgc tttcatatca actttttatt tttgagaatt ttcaatattt
    20521 tatttaagac tacctccata ttttctacgg atttgtagtt gtttttaagt actaatttta
    20581 taatttttta tatctaatga tgagtctggg atattgattc atgtctctaa cactttcgat
    20641 aatcccccta cccctaccct actgcatcga tattactcat actacatcaa cgaaatcagt
    20701 ttttatcgct taatttccta taatagtttt gctcaaactt gttacgtttt agattgtttt
    20761 agttcatcat aattatcccg tattgttgct ataatgaaat gcattcatcc cattgaacca
    20821 caaacttaat ttattgttgt tatgtgcatt ggctcactat tatactttta cagcacaaaa
    20881 aaagtggcga cagcttcgtc accacttttt aaatataatt caaagtatct tgcccttgct
    20941 ttaagtatac gtagatatat actttttaaa gcttgtagct aaagccttta tttaactggt
    21001 tttgaaattt gtgttttacc acccataaat ggtactaatg cttctggaat tgttactgtt
    21061 ccatcttcat tttggtaatt ttcaacaata gcagcaaatg tacgtccaac tgctaaacca
    21121 ctaccattta atgtatgtgc taattctggt ttagctgctt tgtcacgctt gaagcggatg
    21181 ttagcacgac gcgcttggaa atccgtacag tttgagcatg aactaatttc tttataatca
    21241 ttgtagcttg gtaaccaaac ttctaaatca tatgttttgc ttgcactaaa accaatatca
    21301 cctgtacata aaataacacg acggtatggt aaacctaact cttctagaat tgcttctgcg
    21361 tttgttgtca tttcttctaa agcattccat gaatcttcag gttgttcaaa acgtaccatt
    21421 tccactttat cgaattgatg taaacgaatt aatcctcttg tatctctacc tgctgatcct
    21481 gcttcactac ggaaacatgc agattgacca gtgaattttt caggaagtac acctggttga
    21541 ataatttcat tacgatagaa attcgttaat ggtacttcag cagttggaat tgtatataat
    21601 ccttcttttt ctactttaaa taaatcttct tcaaatttag gtaattgacc tgtaccatac
    21661 attgtatctg cgttcacaag ctgtggtacc atcatttctg tataaccatg ttgtgttgta
    21721 tgttttgtaa tcatatagtt cattaaagca cgctctaatt gcgcaccttc atttgttaaa
    21781 tatacaaaac gcgcacctga aacttttgct gcacgatcaa aatcagccat tttcaattct
    21841 tctacaatat cccaatgtgc ttttggttca aatgaaaact cacgtggtgt accccacttt
    21901 ttaacttcaa cgttatcttc atcagattca ccttgaggta catcatcact tattaaattt
    21961 ggaatacgac aaaggatacc tgtcatttta ttatcaattt catttaattg actatctttt
    22021 tctttaatat cgtcacctaa tgtgcgcatt tcagcaatca catcatcagc attttcttta
    22081 ttacgttttt ttaatgcgat ttcttcactt actttattac gacgtgcttt catttcttct
    22141 gtagcactaa tcaatttacg tcgttgctca tccaattcta aaatttcatc tacaactttt
    22201 ggatcatctc cacgtaattc aattttgctc ttaactgtgt caggctcatt tctgaataat
    22261 ctaatgtcta acattaacct tcatcctttc ccaaataatt atcatttatt atggaatgac
    22321 gcacgtcttt attttttaga aaataaaaaa agaccacatc cctacaaggg acgtggtcta
    22381 cgcgttgcca ccctatttaa caatttaagt tataaagata cactaaacct aaattgcact
    22441 tcactaaaat aacggttatc accgattgtt cttttaaatg agtaggtaga ttcatatata
    22501 tgttgattct tgttcacact aaccacaagc tctctgatat cgaacactat atattacttg
    22561 tcctacgaac aatgtcttat taagttattt ttaatatagc aaactatatt tgctttttca
    22621 agtaacgatt tcaaacatca atcatgtcga tttaatgaca tgcagtcgtt tgataaattg
    22681 attgctttaa atactgtgca accgcttcaa tatctttatg aaattgacgg tcatgtgtaa
    22741 tggatggcac gatacttcga aactcatcat acttgcgacg tgtttttggt gataatcctt
    22801 cgacaccttt caactctgct gcttgtaatg caataacaca ttcgattgcc aacacacgtc
    22861 ttgcattttc aataatttga taaccatgtc tagcagctgt agttcccata gatacgtgat
    22921 cttcttggtt cgcagatgaa gtgatagaat caacactcgc tggatgcgct aaagttttat
    22981 tttcagaaac gagacttgca gcagcatatt gcataatcat cgcgccactt tgcaatcctg
    23041 gctctggact aagaaatgct ggtaaatcac catttaattg aggatttact agtcgctcta
    23101 gacgacgttc cgatacgttt gctaattcac ttacacctaa tttaagatga tctaatgcaa
    23161 aagcaatagg ttgtccatgg aagttaccac ctgaaataac aaacgtttca tttgcttcct
    23221 caaatataag tggattatca ttagccgcat tcatttcaaa ttccaattgc tgtttaacat
    23281 aattgaatac ttgaaaactc gcgccatgga tttgtggtat acaacgcaac gtatatgcat
    23341 cttgtacacg tatttctgct tgtcgcgtcg ttaatgttga tccttctaac caatcacgca
    23401 tacgcgctgc cacattaatc tgttcttgaa aattacgaac agcgtgcaca tcatgtcgat
    23461 atgcatctat aatgccatta agagactgat gcgttaatgc agcaatccat tcagattggt
    23521 aacctaaatc ttctgcttct atataactaa tgacaccttg agctgtcata gcttgcgtac
    23581 cattaatcaa tgctaaacct tctttagcct gaaggttcaa aggttgtcta tttaattctc
    23641 ttaatacatc gtcactatcc ttttcttccc ctctgtgcaa tactttccct tcaccaatta
    23701 atgctaatgc taagtgtgat aatggtgcta aatctcctga tgcaccgagt gacccttgtt
    23761 gcggtattat cggtataata cgttcattta taaaaaattg taattgtctc actaattcta
    23821 aagtggcacc tgaatgacct tttaataatg tattcaatcg taaaatcatc atgactaatg
    23881 ctacttcttt tgaaaatggc tcacctagtc cacaggcatg tgagcgtatc agattcactt
    23941 gtaattcatt atattgcgtc gggtctatac gtacatcact aaataaccca aaacctgtag
    24001 tgataccata aaccgtttcc tcattttcaa taataccttc aactaccgct ctactttttt
    24061 tgacacgttc taacgcatca tcaataattt caatctttga ttgttgttgt aaaaatgatt
    24121 taatatcctc aattgttagt gtttcaccat ctaaatataa agtcatatat gttaccccct
    24181 tgtttatatt aagtaaccca tccttcttga agtatacgtt ttcattttta ttgaaacaat
    24241 ggttttacgt acatttataa cctattatca gagcactatt gtagtgcatt aaaggatatt
    24301 aagattgt
//
LOCUS       DAPIDM010000027        24218 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000027 DAPIDM010000000
VERSION     DAPIDM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.027"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..624
                     /gene="yjbH"
                     /locus_tag="RJE42_000968"
     CDS             <1..624
                     /gene="yjbH"
                     /locus_tag="RJE42_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDV5876408.1"
                     /translation="NPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIHLMQ
                     NEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIEQAP
                     SLVFFSEDVHDEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLVTME
                     ELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(1084..2892)
                     /gene="pepF"
                     /locus_tag="RJE42_000969"
     CDS             complement(1084..2892)
                     /gene="pepF"
                     /locus_tag="RJE42_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDV5876409.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEESGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2940..3926)
                     /locus_tag="RJE42_000970"
     CDS             complement(2940..3926)
                     /locus_tag="RJE42_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDV5876410.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNESIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPISYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIMMFQQIQYIGGHKYVAEKRNVKMSELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGESILVSDNLNYFKLKKFIVALESKTAIIEKLINNYLNISSDRGNDVQILF"
     gene            complement(4047..4766)
                     /gene="mecA"
                     /locus_tag="RJE42_000971"
     CDS             complement(4047..4766)
                     /gene="mecA"
                     /locus_tag="RJE42_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDV5876411.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDSTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(5137..5532)
                     /gene="spxA"
                     /locus_tag="RJE42_000972"
     CDS             complement(5137..5532)
                     /gene="spxA"
                     /locus_tag="RJE42_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDV5876412.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            5827..6816
                     /gene="trpS"
                     /locus_tag="RJE42_000973"
     CDS             5827..6816
                     /gene="trpS"
                     /locus_tag="RJE42_000973"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV5876413.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(6936..8558)
                     /locus_tag="RJE42_000974"
     CDS             complement(6936..8558)
                     /locus_tag="RJE42_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5876414.1"
                     /translation="MEISKLKYLLLNFIFVLILSGCSKEGIYSDKGQVFRKVIPQDMS
                     TLDTTLITDAVSGDISNQVFEGLFTLDKNDKAKLALAKELPKKSNGGKTLTFKLRKDA
                     KWSNGDPVTAYDFVYAWRKLVDPKTGSEYAYIMSDIENADSINSGKLPVEKLGIKALS
                     KYELEIKLKRPIPYINELLALSNFYPQNEKVAKKYGEKYGLKPQNAVYNGPFKVDTWR
                     AEDKILLSKNNFYWDKKNVKLDKVNYKILKDKQAGASLFETGSVDDTLILADQVKKYE
                     NSSALQKRITSGNFFIKFNQKKVEEFKNQDLRMAIALAIDKKGYVEAVKNDGSIYSNT
                     FTAKGVAKLSNGDDYLSSINSPLKYDKKEALKHWEKAKKELNIKNFTFSMNTEDTPDS
                     KISAEYIKAQIEKNLPGVKMKIRQLPFKQRLSEEFKMTYETSLTGWAADYPDPTTYLS
                     TMTKDNPQNNTDWSNKKYDDYLGEINGELLGEVSKRDNRMKEDEELLLNDVPLAPIYQ
                     KGEAHLTNPKVKGLQYHVVGPDTTLKYVYIDK"
     gene            complement(8586..9512)
                     /locus_tag="RJE42_000975"
     CDS             complement(8586..9512)
                     /locus_tag="RJE42_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876415.1"
                     /translation="MNNNVLVEIKNLTKIFNKNKNNEIKAIDDVSININKGEALGIVG
                     ESGCGKSTFGKSIIRLLEVPEETIFFEGKDINKITSRRDSLNLNKRIQMIFQDPYASL
                     NPRLKVMDIVGEGIDIHKLAENKAERNEKIYKLLELVGLNKEHANRYPHEFSGGQRQR
                     IGIARALSVEPDLIIADEPISALDVSIQAQIINIIKKLQKERNTTLLFIAHDLSMVKY
                     VSDRIAVMHKGKIVEIASSERLYQNPLHNYTKSLISAIPQPDPQYERERVREIYKDSF
                     DNNSEYKLREIEKDHFVFTSINKAELLRQRKL"
     gene            complement(9502..10581)
                     /locus_tag="RJE42_000976"
     CDS             complement(9502..10581)
                     /locus_tag="RJE42_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876416.1"
                     /translation="MKKVLEVKNLEVSFEVEDGIVNAVRGIDYCIHEGEILAIVGESG
                     SGKSVATKAITKLFQGENGKITNGNVKFFGEDLVEKNEKELAKIRGKDIAMIFQDPMT
                     SLNPTMQIGKQIMEPLMIHKKMNRKDAKGKAVEILDLVGIKNAELRFKDYPHQFSGGQ
                     RQRIVIGMALACEPKLLIADEPTTALDVTMQSQIIDLMKILQKKTNTAIIFITHDLGV
                     VANIADIVSVMYCGEIIETGKVEELFYYPKHPYTWGLLASMPTLESNSKSKLIAIPGT
                     PPNLLKPPIGDAFARRSAYALNVDFKYEAPWFQLSPTHFVKSWLLDSRAPKVIPPETV
                     KRKFKELSYNFERPLKVEGVSFNEQ"
     gene            complement(10597..11616)
                     /locus_tag="RJE42_000977"
     CDS             complement(10597..11616)
                     /locus_tag="RJE42_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876417.1"
                     /translation="MVIDENKKQLTSTDFEFKKDKINQEFLNVKNKNFWQDGWSQLRK
                     NKLAVIGMIGLIMIILFALFAPAVSQHNSTEQNVTDRNLPSKIPLLEKVDYLPFNGKD
                     VNGKDTYKEVGSKDYYLFGTDQLGRDLWSRTWEGARISLFIGVVAAILDIFIGVLFGA
                     ISGFYGGKLDTIMQRIIEIIASIPNLIIVILFVLIFEPSIWTIILAMSVTGWISMSRV
                     VRGEFLKIKNLEFVQASKTLGVSNLRLIFKHILPNTLGAIIVTSMFTVPSAIFFEAFL
                     SFIGIGVPAPQTSLGSLVNDGKSMLLIYPHELFIPATILSLLILFFYLFSDGLRDAFD
                     PKMRK"
     gene            complement(11606..12541)
                     /locus_tag="RJE42_000978"
     CDS             complement(11606..12541)
                     /locus_tag="RJE42_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876418.1"
                     /translation="MIKYFLKRIFYMAISLVIITTITFFLMKLMPGSPFNDAKLSPDQ
                     KYILNEKYGLNDPIPIQYLNYMKNVFTGDFGYSFQYHNQPVWDIIKPRLIPSLEMGIT
                     AMIIGVLIGLILGVASAAKQNTWIDYCATLISVLAVSVPSFVLAVLLQFVFAVKLRWF
                     PVAGWEGISTVILPSFALSAIVIATVTRYIRAELLEVLKSDYILLARAKGVPNKRVLY
                     THALRNALIPLVTVLVPMMASILTGTLTIENTFGVPGLGDQFVRSIATNDFPVIMATT
                     ILFSSFFIVSIFLVDMLYIIIDPRIRIQGGRNNGD"
     gene            complement(12711..14366)
                     /locus_tag="RJE42_000979"
     CDS             complement(12711..14366)
                     /locus_tag="RJE42_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5876419.1"
                     /translation="MTRKLKALILILVATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKKVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNELSFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            complement(14385..15326)
                     /locus_tag="RJE42_000980"
     CDS             complement(14385..15326)
                     /locus_tag="RJE42_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876420.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFEIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEDEAARLRKNLLVIQD"
     gene            complement(15316..16398)
                     /locus_tag="RJE42_000981"
     CDS             complement(15316..16398)
                     /locus_tag="RJE42_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876421.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            complement(16414..17484)
                     /locus_tag="RJE42_000982"
     CDS             complement(16414..17484)
                     /locus_tag="RJE42_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876422.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPLLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            complement(17484..18410)
                     /locus_tag="RJE42_000983"
     CDS             complement(17484..18410)
                     /locus_tag="RJE42_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876423.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGMILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            18653..19024
                     /locus_tag="RJE42_000984"
     CDS             18653..19024
                     /locus_tag="RJE42_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDV5876424.1"
                     /translation="MLKKWLGMALITPMLTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSKWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(19079..20323)
                     /gene="fabF"
                     /locus_tag="RJE42_000985"
     CDS             complement(19079..20323)
                     /gene="fabF"
                     /locus_tag="RJE42_000985"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV5876425.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLDSAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQNLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(20335..21276)
                     /locus_tag="RJE42_000986"
     CDS             complement(20335..21276)
                     /locus_tag="RJE42_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDV5876426.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            21571..21756
                     /locus_tag="RJE42_000987"
     CDS             21571..21756
                     /locus_tag="RJE42_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDV5876427.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(22013..22447)
                     /locus_tag="RJE42_000988"
     CDS             complement(22013..22447)
                     /locus_tag="RJE42_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDV5876428.1"
                     /translation="MKLKSFITVTLALGMIATTGATVVSNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(22758..23273)
                     /locus_tag="RJE42_000989"
     CDS             complement(22758..23273)
                     /locus_tag="RJE42_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDV5876429.1"
                     /translation="MKINTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVTELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLADDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            23404..>24218
                     /locus_tag="RJE42_000990"
     CDS             23404..>24218
                     /locus_tag="RJE42_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5876430.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPIYITKNSNYNISVINTKILQAPISFTYIYSKKENP"
BASE COUNT         7801 a         4325 c         3286 g         8806 t
ORIGIN      
        1 aatccttcgt taaaagtatt aacgaaatgc caagctcaaa gtacatccaa ctttgataac
       61 atcgccctag cttataaagc agctgagtta caaggtcgtg tacgtgccga acgatttata
      121 catttaatgc aaaatgaaat catacctaaa agagatatta ttacagaatc aatgatttgt
      181 gactgtattc aaaatgcagg tattgattta gaggtattta aagacgactt acaaaaaagt
      241 aaactaaccg aaagcttgaa aattgatttg catattgcaa gagaaatgga aatcgaacaa
      301 gccccttctc tcgttttctt tagtgaagat gttcatgacg aaggtttaaa agtcgaagga
      361 ttatacccat atcacatcta tacttatata attaatgaat tgatgggtaa acctatcgaa
      421 aagaatcttc ctcctaaatt agaaacttat atacagcaac aacaacttgt aacgatggaa
      481 gaattactta ctatttatga atggccagaa aaacttttaa acaaagagtt aaagaagtta
      541 gctattcaac aaaaaattga aaaacttaaa tatccagatg gcgatttctg gaaatctaaa
      601 atgcctaaaa tcaaatctaa ataaataaat taaagacctc ttacaccata aatgtaacga
      661 ggtcttttta ttgactctta attttgcaac attaaattat tagaacgtaa tgttacgcat
      721 cgatctaaat atattagtga tgtttttcga atttaaccgg ttcgaaaatt atacaaatta
      781 tgaatttggt acataataga catatattaa tcgttaatgt ataaaaagtg catatttaat
      841 acatttttat acaacttcac ttccctctta ttaaaaaaga acataaaaaa acgtcgtgtc
      901 tgaatacacg acgctaaaca aaggggatgg gagaaatttt tcacttcaaa caaaggggta
      961 tgtttgttat gtgattaatt tcatgttcat aatataacac gctatctctc ccatttcaac
     1021 cttttaatag tacttaattt aaattgtcac aatctgttca taatgtaagc gaatacaaca
     1081 gcgctaagct ttcattaatt tttcaaaagc gttcaatttt tgttcaaaaa cttcacaagc
     1141 ttgttcaatt ggttcaggtg ttgtcatatc tacaccagca ttttttaata tctcaattgg
     1201 ataatttgag ctaccttttt tcaagaattc attaatatat ctatctactg ctggcttacc
     1261 ttctgttaaa atttgatgac ttaagctttg agctgcactg taaccagttg cgtattgata
     1321 tacataataa ttcatataga agtgtggaat acgtgaccat tccttactaa tatcttcatc
     1381 agtttctaca gaatcaccaa agtataattt atttaatttg gcatattctt cattcattct
     1441 agttggcgtt aatggttcac ctgattcttc aattgcatga attttatgct caaattctgc
     1501 gaacattgtt tgtcggaata atgtagctct gaaacgttct aattcttggt ttaataataa
     1561 taagcgtttt tcatcatcaa gatgtttatc catataatca cttaaaagcg cttcgttaca
     1621 agttgatgct acttcagcga caaaaatagt gtagtcacta gaatttgacg gttggaattt
     1681 tctactgaag taactatgtg ctgaatgtcc aaattcatga actaatgtgt ataagtctga
     1741 aatagtatta gaccagttaa gtagaataaa tggattagtt aaatgtgcac ctgatgaata
     1801 gccacctgaa cgtttacctt tattctcata gacatcgacc caacgattgt ttaagccttc
     1861 tttaactaca tttaaatatt cttcacccat tggttctaat gcttttaaca tccactcttt
     1921 agcttcttca taaggcattt caaacttaat atctttaatt aatggtgtat ataaatcata
     1981 cattttcaag tcatctaaac ctagcaattc tttgcgcaat tcagtatatc tatgtagcaa
     2041 tggtaaatat ttatgtacag tttttactag attgtcatat acattttctg gaatatgatt
     2101 attactcaat gctttttctc ttgcagtttt gtaattgtgt gtacgagcat taaatacatt
     2161 tttcttcact tcacctgcta gcgtagcacc aagcgtatta ttatgagcac catatgcttt
     2221 atatacattt ctaaaagcac tttctcttag tttgcgatca tctgattcta aatacttaat
     2281 aaatgtacct tgtgttaacg ggtgtgcatt tccatcttta tcaatcgcat cttcaaatac
     2341 taaatcagcg ttgctaaaca taccgtatac atttgatggc gttgataatg cgtcctgcgc
     2401 ttctgttaat aacttttcag tttcagcatc taaaatatga ggacgttttt cattaatcaa
     2461 tttcaaatca aaagcaaatt tttgtaactt atcatatgaa tttacaaaag attgaatttt
     2521 atcttcatca atttgtaaaa tctctggcac taagaaactc catgccgagc taaatttaat
     2581 aattaattga tgtgctcttg actccatacc agtatatttg tcgttcgttg tatcttggtc
     2641 ttgttttagg tgcgcgtata catatacttt ttctaattta gtacctaatg tatcttctaa
     2701 ttctaacgca ttgtataatg tctcagcact atcaccaatg tgtcctttaa attgttcttc
     2761 tttgcctaac tcattttcaa cttctttaaa tgcagcctca aaagcttcat catctttgaa
     2821 aattgttgtt aagtcccatg tatattcagg atatttacgt tcctgttctt ctctcgataa
     2881 ttgttgactc atatgtagta aacctcctaa taaaatataa ttgatataat tttctcattt
     2941 tagaacaaaa tttgcacgtc attacctcta tctgaactaa tatttaaata attgttaata
     3001 agtttttcta taatcgctgt tttactttct agagcaacaa taaacttttt cagtttaaaa
     3061 taattaagat tgtcacttac taatatgctt tctccattct ttaaccataa atcaacttgt
     3121 aattgccact caattggatg attttcaata taaatttgct ctggaaaaat ataaccataa
     3181 ttgtgcacta cttcttgatc agttaactgt aattgataca ttgcacttaa agtgggttct
     3241 aaaacagaat tttgccagcg acaatatttg atatattgat taattgcgaa ttttgataat
     3301 ttataaacag catgataatc acaaggcgcc tcattaaaca actcactcat tttgacattc
     3361 cttttttcag cgacatattt gtgcccgcct atatattgta tttgttgaaa catcattatt
     3421 tgttttttct ctaaattgaa cgtatgaagt tttcgagtga ttggattaat aaacatactt
     3481 tgaaaatgat tgaacttcac tttattatga caataatcaa catcatttaa taaccaactt
     3541 actttatatc ccattttctt taaaccttcc gttcgttgaa gaatctgttt ataagaaatt
     3601 ggcgaatact gtagctcaat aacatattta ttattaatca aaatatccgg aatttgtttt
     3661 atttctttta aaaatggctc tatctctact ttacatcctt gttgctgtat catctgtgct
     3721 aaaatcaatt tacttttata atgtttaatc gattcattat taaaacattt ttgttccgct
     3781 aaatgtttat gtgcaaaatg actaattact ttgagcccac gctttaaaat aacttgcttg
     3841 ccacacaccg gacaaaaata ttgtgtcttt ctcaatgcag tagttgctaa cacgcgttcc
     3901 ttttcttcat ttaaagctac taacatatct tcacctcaaa atattacacg taagtttaac
     3961 tactattact ttttcagctt ttgaaatcaa taaaaaaaga gactccaaag agtctccttt
     4021 catagttcgt tgcttaatta atatcattat tcagttgtct ctggaaaata acgtcgaact
     4081 tgagctgtta cgttatgact cataattatt ttagcatagt catttaaata aacttctgtt
     4141 ctgtctgttg gataagcaaa ttcaagcaat tgactgtaac tatcattaat gacctcttga
     4201 tcaacatgac tatcaaaatg tacagcataa taataagtac tatcaaccat atataacaaa
     4261 tcttcaaact ctgtagttat tggattgcta tgatatgcat aattaataac atcttctaaa
     4321 tcgttaaatt tcacaatgat tgttcttgta tttttacgtg ctgaagactt ttgacgttta
     4381 gaaccttgag cctctttttc ttttgttcgt tgctcgaata attcttctaa ttgatcttca
     4441 ccttctaatg tttgagctaa caattcttga acttgttcat caaattgatc agttgcatca
     4501 tcatcagaca tattcatcat atcttcattt ttagatttag aaattgtaac ttcgacacct
     4561 ttttcaaagg catgtacttg aatccataat ggaccttcta caacaaaatc ttcttcttcg
     4621 ttaatttcat ccatcattga ccaaaagaat tcttctccac gtttgcgatt tgtccataaa
     4681 tcttcacgac taaatccacg ggcctcgata tcgctatatg ttataaacaa ttttacagtt
     4741 gtatcatcta ctcgttctat tctcatatca tctcactcct tacagtcgat gaatagtatc
     4801 cacattgtat taaagcatca aacaaaatac aatatatttg tttgatttct ttctctaaaa
     4861 tattttggta acttataata acatattcgt tgtagcaaaa ctatttaaat gatttataca
     4921 gttaaagcat taaagcactt tcttaattta attttacctt atcatagcaa atgtttcatc
     4981 caaaacactc taatatagag ctatattaac aattgcacat ttgatttaaa agatactaag
     5041 caattattgt aaaattaaaa ccaaaaaagc aacactatag aaatagtgtt gcttgacata
     5101 tcttttagta aagtatgaca catagttaaa tggttattag tcaaccatac gttgtgcttc
     5161 ttgtaattgg aacgtacgaa cttttctagg taagaaacgt cgaatctcgt cctcattata
     5221 accaacttgt agtcgtttat tatctaaaat aattggacga cgtaataagc caggattatc
     5281 ttgaatgatt gaatataagt cttgtaatgg tagtgaatca atatcaacat ttaatttttg
     5341 gtatgtttta gaacgtgtag aaatgatttc atcagtaccg tcttcagtca tttttaatat
     5401 ttgcttaatt tcatcaattg ttaaatgttc agaaaaaata ttacgctccg tatacggaat
     5461 gtcatgttct tgtaaccatg ctttcgcttt acggcaagat gtgcaacttg gtgaagtaaa
     5521 taatgttacc atacatctca ctctcctatt tgaatgaata aaattcattg cttaaaattt
     5581 agttatagat caagaaaaaa catttttttc taaaattctt aatcgttact atttattata
     5641 actatctaac attaaaatta aatgagaaaa acctaatttt tcagataagt ttctacactt
     5701 ataaaaaaga ttattaatcc ctttgttagt agcaagttat acgtatattc taacacatct
     5761 tacattttta agaaatactg ttataatgat aattattaaa atattactaa gaaagtaggc
     5821 atttaaatgg agacattatt ttcaggcatc caacctagtg gaattcctac tattggaaat
     5881 tatattggcg cactaaaaca atttgttgat gtgcaaaatg actacgattg ttatttctgt
     5941 atcgtagatc aacatgcaat tacaatgcca caagatcgct taaaattacg taaacaaact
     6001 agacaattag cagcaattta tttagcttct ggtatagatc cagacaaagc aacattgttc
     6061 atacaatctg aagtccctgc acacgtacaa gcaggatgga tgttaactac gattgcttct
     6121 gttggcgaat tagagcgtat gacgcaatac aaagataaag ctcagaaagc agttgaaggt
     6181 ataccagctg gtctattaac atatccacct ttaatggcag ctgatattgt tctttacaat
     6241 actaatatcg ttccagttgg cgatgaccaa aagcagcaca ttgaattaac acgtaatctt
     6301 gtagatagat ttaatagtcg ttataatgat gtccttgtga aacctgaaat tcgtatgcct
     6361 aaagttggtg gccgtgttat gagtttacaa gatccaacaa gaaaaatgag taaaagtgat
     6421 gacaacgcta aaaactttat ttcattatta gacgaaccaa atgttgctgc taaaaaaatt
     6481 aaaagtgctg tgactgattc agacggtatt attaaattcg accgcgacaa taaaccaggt
     6541 attacaaatt taatttcaat atacgctgga ttaacagaca tgccaatcaa agatattgag
     6601 gcaaaatatg aaggcgaagg ttacggtaaa ttcaaaggcg accttgctga aatagttaaa
     6661 gcatttttag tagaattcca agaaaaatac gaaagtttct ataactcaga taaacttgat
     6721 gatattttag atcaaggtag agataaagca cacaaagttt catttaaaac tgtcaaaaaa
     6781 atggaaaaag cgatgggttt aggacgtaag agataaaata aacataaata cagaaataaa
     6841 acagcagtaa gtaaacttct aattgaaatt atcttactgc tgtttttcac aatgtccatt
     6901 ttttataatc acattttatt agcctttaaa agttattatt tatctatata tacatacttt
     6961 aatgttgtat ctggacctac aacatggtat tgaagacctt ttactttagg attagttaaa
     7021 tgtgcttctc ccttttgata aattggagct agaggcacat catttaacaa taactcttca
     7081 tcttctttca ttctattatc tctcttgcta acttccccta ataattctcc gtttatttca
     7141 cctaaataat cgtcatattt tttattactc cagtctgtat tattttgggg attatctttt
     7201 gtcattgtcg acaaataagt tgttggatcg ggataatcag cagcccaacc agttaatgat
     7261 gtttcatatg tcattttaaa ttcttcactt aatctctgtt tgaaaggtag ttgtctaatt
     7321 ttcattttta caccaggtaa atttttttct atttgtgcct taatgtactc tgctgagatt
     7381 tttgaatctg gtgtatcttc agtattcatt gaaaatgtga agttttttat atttagctct
     7441 ttctttgctt tttcccaatg tttcaacgct tctttcttat catactttaa tggtgaattt
     7501 atacttgata aataatcatc accattacta agttttgcga cacctttggc tgtaaatgta
     7561 ttactgtata tagaaccatc atttttaact gcttcaacat atcctttttt atcgattgct
     7621 aatgctattg ccatgcgtaa atcttgattt ttaaattctt cgaccttttt ttgattaaat
     7681 tttatgaaaa aattacctga agttatacgc ttttgtaaag cactagaatt ctcatatttt
     7741 tttacttgat cggctaaaat taatgtatca tcaacagatc ctgtttcaaa taatgaagca
     7801 ccagcttgtt tatctttcaa tattttataa ttaactttat ctaattttac gttcttttta
     7861 tcccaataga aattattctt agacaaaagt atcttatctt ctgctctcca agtatcaacc
     7921 ttaaacggcc cattataaac tgcattttga ggtttcaaac catacttttc tccatacttt
     7981 ttcgcaactt tttcgttttg tggataaaaa ttacttaaag ctaacaattc atttatgtat
     8041 ggtataggtc ttttcaactt tatctcaagt tcatatttac ttaatgcttt aatacctaac
     8101 ttttcaactg gtaatttacc agaatttata ctatctgcat tttcaatgtc agacattatg
     8161 taagcatatt cagatcctgt ttttggatca actaattttc tccacgcata tacaaaatca
     8221 tacgctgtta ctggatcacc gttagaccat ttagcgtctt ttcttaattt aaaagttaaa
     8281 gttttaccac cattactttt tttaggtagt tctttggcta acgctaattt cgctttatca
     8341 tttttatcta gtgtaaaaag tccttcgaat acttgatttg atatatcacc tgaaactgca
     8401 tctgttatta aagttgtatc taaagtactc atatcttgtg gtattacttt cctgaaaact
     8461 tgtcctttat cagaatatat accttcttta ctacaaccag aaagtatcaa tacaaaaata
     8521 aaatttaata ataagtactt taattttgaa atttccataa tttagtttcc ccctttctaa
     8581 aagcattaaa gttttcgttg tctcaatagt tctgccttat ttatacttgt gaacacaaaa
     8641 tgatcttttt ctatttccct tagtttgtac tcagaattat tatcaaacga atccttgtaa
     8701 atttccctaa ctctttctct ttcatattgt gggtctggtt gaggtattgc tgaaattagt
     8761 gattttgtat aattatgtaa tggattttga tatagtcttt cagaactggc aatctcaact
     8821 attttccctt tgtgcatgac tgcaattcta tctgaaacat atttaaccat tgataaatca
     8881 tgtgcaataa ataaaagcgt agtattcctt tctttttgta attttttaat gatatttatt
     8941 atttgtgctt gtatcgatac atccagagct gaaataggtt catctgcgat aatcaagtct
     9001 ggttcaacag atagtgctct tgcaattcca attctttgcc tttgtccacc tgaaaattca
     9061 tgcggatagc gatttgcatg ttctttattt agacccacta attctaataa tttataaatt
     9121 ttttcatttc tttcagcttt attttccgcc aatttatgta tgtctatccc ctcacccaca
     9181 atatccataa ctttaagtct aggatttaat gaagcataag gatcttgaaa aatcatttga
     9241 atccgtttgt ttaagttaag gctatctctt ctagatgtaa ttttgttaat atcctttcct
     9301 tcaaagaata tagtttcttc tggtacttct aataatctaa taatggactt tccaaatgta
     9361 gattttccac atccagattc tcctactata ccaagcgcct cgcctttatt tatattaatg
     9421 ctcacgtcgt caattgcttt tatttcatta tttttatttt tattaaaaat tttggttaaa
     9481 tttttaattt caacaagtac attattgttc attaaatgat actccttcta cttttaacgg
     9541 tctctcaaaa ttataagaaa gttctttaaa tttacgctta acagtttctg gcggaattac
     9601 ttttggagcc ctgctatcta gtaaccaaga ttttacgaaa tgtgtaggtg ataattgaaa
     9661 ccatggcgct tcatatttaa aatcaacatt taacgcatat gcacttcttc ttgcaaatgc
     9721 atcaccaata ggtggcttca acaagttagg tggtgttcct ggaattgcaa tcaattttga
     9781 ctttgaatta gactctaatg tcggcattga agcaagaagc ccccatgtat atggatgttt
     9841 gggataataa aacaattctt ctacttttcc tgtctcaata atttcaccac aatacataac
     9901 tgacactatg tctgcaatgt tagcaacgac tcctaaatca tgagtaataa aaataattgc
     9961 agtatttgtt ttcttttgta atattttcat taaatcaata atttgcgatt gcatagttac
    10021 atctaatgca gtggttggct catcagcaat taataacttt ggttcgcatg ctagagccat
    10081 acctattaca atcctttgac gttgtccacc agaaaattga tgtgggtaat ctttaaatct
    10141 taattcagca ttttttatac ctactaaatc taaaatctct acggcttttc ctttggcatc
    10201 ttttctattc atttttttat gtatcatcaa cggttccata atctgtttac ctatttgcat
    10261 tgtagggttt agtgatgtca ttggatcttg aaaaatcatg gctatatctt ttccacgtat
    10321 cttagcaagt tctttttcat ttttttcaac taaatcttcc ccaaaaaact tcacatttcc
    10381 atttgtgatt tttccatttt ccccttggaa caatttagta attgctttag tagcaactga
    10441 tttacctgat cctgattctc caacaattgc tagtatttct ccctcatgta tgcaataatc
    10501 aattcctctc actgcattaa ctatgccatc ttcaacttcg aaagaaactt ctagattttt
    10561 tacttccaat acttttttca tcattaaaac acacccttat ttacgcattt taggatcaaa
    10621 cgcatcgcgc agcccatcac taaataaata gaaaaataaa attaaaagac ttaaaattgt
    10681 tgcaggtatg aataactcat gcggataaat taatagcatt gatttaccat cattcactaa
    10741 tgaacctaat gatgtttgag gtgcaggtac accaattcca ataaaactta aaaaggcttc
    10801 aaaaaaaatt gcgcttggaa ctgtgaacat cgaagtaact attattgcac ctagtgtatt
    10861 aggtaatatg tgtttgaata ttaacctcaa atttgaaacc cctaatgtct ttgacgcttg
    10921 cacaaattct aaatttttaa ttttaagaaa ttctcctctt actactctac tcattgaaat
    10981 ccatccagtc actgacatag ctaaaataat tgtccatatt gatggctcaa agattaatac
    11041 gaataaaatt actattatta aattaggaat cgaagctata atttctataa ttctttgcat
    11101 gatagtatct aatttgccac cataaaaacc agatatagca ccaaataaaa ctccaataaa
    11161 tatatctaaa atagcagcaa ccacaccaat aaataatgat attcgtgcac cttcccatgt
    11221 tctcgaccat aaatctcgac ccaactgatc ggttccaaat aagtaataat cttttgaacc
    11281 tacctcttta taagtatctt tgccgttaac atctttgcca ttaaaaggca aataatcaac
    11341 tttttctaac agaggtattt tcgaaggtaa atttctatct gtgacattct gctcagtact
    11401 attatgctga gaaactgcgg gtgcaaataa agcaaataaa ataatcatta ttaatccaat
    11461 catacctata acagctaatt tattttttct taattgtgac cagccatctt gccaaaaatt
    11521 cttattttta acgtttaaaa attcttgatt tattttatct tttttaaatt caaaatccgt
    11581 tgaagtcaat tgctttttat tttcatcaat caccattatt tcttcctcct tggatgcgta
    11641 ttcttggatc aataattatg tataacatat ctactaaaaa tattgacact ataaaaaaac
    11701 tactaaataa aatagttgta gccattatta ctgggaaatc atttgtggca atagaacgta
    11761 caaattgatc tcctaaccct ggtactccaa aagtattttc aatagttaat gtaccagtta
    11821 atatactagc catcattggt actagcactg ttacaagtgg gataagagca tttcttaaag
    11881 cgtgtgtata taaaaccctt ttattaggaa caccttttgc cctagccaac agtatataat
    11941 cactttttaa cacctctaaa agttcggcac gaatatatct agtgactgtc gcaataacaa
    12001 tagcagatag cgcaaatgaa ggcagtataa ctgttgaaat gccttcccaa ccagctacag
    12061 gaaaccatct caatttaact gcaaatacaa attgtaataa aactgcaagt acgaaagaag
    12121 gaacagacac tgctaatact gatataagtg ttgcacaata atctatccaa gtattttgtt
    12181 ttgcagcaga agcaactcct aaaattaatc caatcaacac tcctattatc attgctgtaa
    12241 tgcccatttc cagagaaggg attaaccttg gtttgataat gtcccacact ggttgattat
    12301 gatactgaaa agaataacca aaatcccctg taaatacatt tttcatgtaa tttaaatatt
    12361 gtattggtat agggtcattt aaaccatatt tctcatttaa aatatatttt tgatccggac
    12421 ttaatttagc atcattaaaa ggtgaaccag gcattaattt cattaaaaaa aaagttattg
    12481 ttgtaataat tactaatgaa attgccatat aaaaaattct cttcaaaaaa tatttaatca
    12541 tcgcaatcta tacactccat ctaactaaat tttcagaata ttaacactat tatatagcta
    12601 ttttatttat tttcaacatt tttttattgt ttcccatttt ttattcccaa agtttttctg
    12661 caacacaaaa agactggaga tatgtctcca gcctgaattt acaaagcata ttattttttc
    12721 ttcttacctg tttctttatc tatcgattta tcaatatata catgtttaag tgagttattt
    12781 ggaccaaatt tatggtaaat taatcctttt acttgaggat ttgttaaatg tgcaacacct
    12841 ttttgataaa ttggtgctac cggtgcatct cctaggaaca tttcttctgc ttttttcaag
    12901 ttttcatatc gttcgttcgg ttgaagtgcc aatttggttc tcgctacttt aagtaattga
    12961 tcgtattctt tattacccca gtctgtatta ttttgtgcgc tacctgtggt cattgtttct
    13021 aagtaagcca taggatcagg gtaatctgca gaccaaccac taagtgatgc ttcaaaattg
    13081 ttactcagtt ctagtgatac tctttgttta aacggtaatt gcttaatttt caaagtgaca
    13141 cctggtaaat ttttctcaac ttgtgatttg atgtattcag cagatatttt tgcatctggt
    13201 gtatcttctg tgttcatcga aaatgacaat tcatttttac ctaactcttt tttagctttg
    13261 tcccagtgcg ctcttgcttc tttaggatta tattttagag gtgaattcat ggtactcgca
    13321 taatctttgc cattaggtgc tttcgcaatg cctttggctg ttagtgtatc ggaaggaatt
    13381 gaaccattgt ttttcactga atcaacgtat ccttttttat ctattgcttg tgcgatagcc
    13441 agtcttaaat ttttgttttt aaaatcagga tattgttttt cattcatttt tacaaaaaat
    13501 gtcccagtcg ttaacacaaa gtttaatcct ttattatctt tatatttatt tacttgatct
    13561 gctgtaataa ctgcgtcatc tactgattca gtatcataca atgatgcacc tgcttgtaag
    13621 tctttaataa ctttataatt cactttatct aattttactt tctttttatc ccaataatac
    13681 tgatttttag atagtaacgt tttatcttct tgtttccaat catcaacttt aaatggacca
    13741 ttgtataccg ctctatcagc tgccgtacca taatttttac catatttttt ggcaactttt
    13801 tcattttgag gtgcaaatgt attaagtgct aataattgat taatgtatgg taccggcttt
    13861 tctaaatcaa tttgtaatgt ttcatcatct aatgctttga tacctaattg ctctacaggt
    13921 ttcttaccag tactaatatc actcgcattt ttaatgtccc ccataatgta tgcaaattca
    13981 gaacctgttt tagggtcaac tgtttttctc caagcataaa caaagtcttg tgcagtcact
    14041 ttgtcaccat tgctccattt agcatcgctt cttaatttaa cctttaaagt tttaccatct
    14101 ttactctttt caggaaaagc tttcgcaaca cctaacaccg gtttgtcacc ttttcctaat
    14161 gtgtataaac cttcgaatgt ttgcgcagtc acttcagaag atatttcatc cgttattaat
    14221 gatgtatcaa gggatgttaa gtctgatgat aaaatttttc tgaatacttg acctttatct
    14281 gaataaatac catcatcatt agcacaacca cttaatgcaa ttgtagcaac caatattaaa
    14341 atcagcgctt ttaactttct tgtcatttca tttccccctt cctcttaatc ttgtatcacc
    14401 aacaaatttt ttcgtagtcg tgccgcttca tcttcagtac taaagacaaa gtgattcggt
    14461 ctaatttcat gtaattgtct taaatgatta tttgcttcat catcaatata actaaatcgt
    14521 ttgcgactac gttccgattc aggatccggt tgaggaattg ccgacaataa agacttagta
    14581 taatcatgta atggatattg ataaatatcg tctgctggtc cgatttctac aattttccca
    14641 aaatgcatga ctgcaattcg atcagagata tacttcacca ttgataaatc atgtgcaata
    14701 aataggaatg taataccttt ttcacgctgt aattttaata acaaattgac tacttgagct
    14761 tgaattgaaa catccaatgc tgaaataggt tcatctgcta taataaattc tggttcaacg
    14821 gctagtgcac gggcaattcc aatacgttgg cgttgtccac ctgaaaattc atgaggataa
    14881 cgattggcat gttctttact taatccaaca gtttcaagta aatcatagac acgtttttta
    14941 cggtcacgtt tatcagttgc taaatgatgg atatcaatac cttcagctac tatatccatt
    15001 acttttaacc taggatttaa agacgcatat gggtcttgaa aaatcatctg tatcttttta
    15061 ttaaatttaa gcaaatcttt acgtttacga atcttttgta tatcaatacc ctcatacaaa
    15121 atttctccac ttgtaatatc attaagtttg ataattgatt taccagttgt agatttacca
    15181 caccccgatt ctcctactaa acctaatgtt tcccctttgt atatttcaaa cgaaatattt
    15241 tcaatcgctc tcacttcgtt tttctttcct gcgttaaaat attgctttaa atttttaata
    15301 gataatagca cttcatcatt tttcattgaa cgacaccctt tctaccttga gtggtttttc
    15361 ataattatta ggcatcggtt tcatacgttg ttttaccagc tcgggtagtt caacttttgg
    15421 tgcacgtgcg tctaataacc aagatttcac aaaatgtgtc ggtgaaactt taaaccacgg
    15481 cggttctact ttaaaatcaa tatctaatgc atattgacta cgtctcgcaa atgcatcacc
    15541 tttaggtggg tgtaataaat caggtggcgc tccaggaatc gctagtaatg gtgtgtcatt
    15601 tgttgttgat agatcaggca ttgacgataa taatccccat gtatatggat gctttggatc
    15661 ataaaatatt tcgttaacat ctcctgtttc taccatttga ccaccataca taactgccac
    15721 tctatcagca atattcgcaa caacccctaa atcatgcgtt ataaaaataa ttgctgtatc
    15781 gattttttgt tgtagttctt tcattaaatc taaaatttgt gcctgcatcg ttacgtctaa
    15841 tgcagtcgtt ggttcatcag caatgagcac tttaggttca caagctaatg cggttgcaat
    15901 aacaattctt tgcctttgtc cacctgaaaa ttgatgtgga tatgctttaa atcttttttc
    15961 tgcatttggt aaacctacaa gatttagtat ttccaatgcg cgctttttag cttgtgcttt
    16021 actataattt ttgtgtttaa ttaatggttc catgacttgt ttaccaattt gcatcgttgg
    16081 gtttaaagat gtcattggat cttgaaagat cattgaaata tctttgccac gtaatttaat
    16141 taactcattt tcaggttttt ttgctaaatc ttcccctaaa aataaaattt ctcccttttt
    16201 aattcttcca gtgtcccctt ggaataattt tgtaattgct tttgttgtta cagatttacc
    16261 tgaacctgat tcaccaacaa ttgccaatgt ttcccctttg tttaaataaa aatctacacc
    16321 tctcactgcc tgcacttccc ctgctgtaat atcaaaggaa acatgcaaat catttacttc
    16381 taatattctt tcagtcatat gctatgcccc cttttattta cgcattttcg gatcaaatgc
    16441 atcacgtaat ccatcactaa ataagtaaaa gaatagaatt aataaactta aaatcattgc
    16501 tggtataaat aattcatgtg gataaattaa taacattgca cgcccatcat ttactaatga
    16561 ccctaacgat gtttgaggtg cgggtacacc tataccaatg aaacttaaaa atgcttcgaa
    16621 gaaaatagca ctaggtactg taaacattga tgtaaccacg atagcaccta acgtattagg
    16681 taaaatatgc ttaaatatca atttgaattt tgaagccccc aatgttttcg aagccatgac
    16741 aaactcttga tttttcaatt ttaaaaattc tccacgtaca actctgctca tgcctaacca
    16801 gcctgtgata gacatagcca atataattgt ccaaatggat ggttcaaaaa ttaatacaaa
    16861 taaaattacg acaattaaat tcggaataga tgctatgact tcaagtatac gttgcataat
    16921 cgtatcgaca cgtccaccga agaatccaga aatcgcacca tatacaacgc caataaaaat
    16981 atctaacatc gctgcaacaa caccgataaa caatgaaatt tgagcacctt tccatgttct
    17041 tgtccataaa tctcgaccca actgatcagt accaaaccaa taattttctt tagcatttgc
    17101 tgctttataa gcatccttgc catctgcatc tttaccatca aaaggtaaaa atggaacttt
    17161 gtctaataaa ggtatttttg ctggaagatt tctatgttgt acattttgtt cagcataatc
    17221 atgtttattt ataactggac cgataaaagc aaatattaca atgataatta aacctatcat
    17281 accgacaaca gctaacttat ttcgttttaa ctgagcccaa gcatcttgcc aaaagttttt
    17341 gctttctcgt tgcatttcag gttcctgatt caaatctaat tctctaataa taaaatcaga
    17401 tgatgcgata gcgtcagagg tatgcgtcat agctgcatta gaatgatcgt cgttaatcga
    17461 caaattgttt ttattttcag ccattatttt ttacctcctt gaacacgaat tcgtggatct
    17521 atcacaccgt acaaaatatc tacaataaaa atagaaacga taaacagtgt gctaaataat
    17581 agtgtgattg ccatgattac tgagaaatca tttgttgtaa ttgaacgtac gaattgatcc
    17641 cctaatccag gaactccaaa aatattttca attgttaaag tgcctgttaa aatacttgct
    17701 aacatgggaa cgataattgt aataattgga attaaagcat ttctaagtgc atgtccaaaa
    17761 agtacacgca ttgtcgaatt acctttagct ctcgctaata aaatgtagtc tgaacttaat
    17821 acctctatca tctctgctct tatgtatctg gcgacagttg ctaaaacagc tgcagataat
    17881 gcaagtgacg gtaataccgc ggtcgaaaaa ccttcccatc cagctactgg gaaccatctc
    17941 aatttaactg caaatacata ttgtaaaagt acagcaagta caaaagatgg tacagatact
    18001 gcaataactg aaataactgt agttgtatag tcaacccaag aattttgttt agtagctgct
    18061 gcaacaccta aaataagtcc cagtatcata ccgatgaaca ttgctgtaag acccatttca
    18121 aaagaaggta gtagtctcgg tttaatcaaa tcccacacag gttgattatg atactggaat
    18181 gaattaccaa aatcgcctgt aacaacattt tttaaataat gtaaatactg cgtagctaca
    18241 ggatcattta atccatattt ttcatttaaa atttcttttt gttcagcatt taatttagca
    18301 tcgttaaatg gcgaacctgg cattaatttc attaagaaaa atgtaattgt aataataata
    18361 aataaagaaa taagcatata aataaatcgt ttaaaaatat atttccccat ctaatcccct
    18421 cctctaacaa attccgattt ctaaatttcc attcccttca aaattttctg aaatcttaac
    18481 taatattata tatacaatat ttactatata tcaatatttt tttgctttaa attaatgaag
    18541 aatgattgat tttagaattt caataaaggt gaagaaatta aaaaatcatt cctcaatgtc
    18601 tcattttaat gtaaactatg cgtaagagtt ttctataagt gggagtgatc aaatgttgaa
    18661 aaaatggctt ggaatggcac taataacccc aatgttaacg tttattatat gggtttttaa
    18721 tagccatact atcattacat atttaaatat attgttttat gtttcattaa ttatttttat
    18781 tagcattttt ttgattttac ttgtacaaga aggcattttt gatgcgacaa gttatggttt
    18841 tagaagatta aagtatcaaa tgtcatcttc gaaaaagaag aagtctatct ctgatgatcc
    18901 atttttcaac ccacaagagg taaaaaaaga gcattacttt gtttctaaat ggataatacc
    18961 attattatta ataaatattt tatattttat tatgacgatt gtgctgtcgc tcatcttagt
    19021 ataaagaaac tgaaaaaatc ttcagaaact aaaacaacgt tactgaagat ttttataatt
    19081 atgcttcaaa tttcttgaat actaatactg cgttatgtcc accgaatcct aagctgttac
    19141 tcattgcata agtaatatca aggttttgcg cttcatttgg aacaatatcc aaatcacatt
    19201 ctgggtctgg tgttaccgca tgaattgtcg gtgcgacttt agagtcttta attgaaagcg
    19261 ctgagaagat tgcttcaatt ccacctgttg caccaagtaa gtgaccagtc attgattttg
    19321 ttgagctaac ttttaagtgt ttagctgctt caccaaatgt atttttaata gctttaactt
    19381 catttaagtc accaacagga gtacttgtac catgggcatt taagtattgt acatctttag
    19441 gttcaatacc agcatcatcc atagctgctt gcattgctct agaaccgcct tcaccttctg
    19501 gagctggcgc tgtaatatga taagcatcac ctgtagtacc atagccaact atctcagcat
    19561 aaatattggc acctcgagct tgtgctgaat ctaaagattc gattactaaa ataccagcac
    19621 cttcacccat aacaaaaccg tctctacctt cttggaatgg acgacatgct gtttcaatgt
    19681 catcatttgt agaaagcgct cgacttgcac tgaaacctgc aattgccata tgagtaattg
    19741 gagcttccgt accaccagta atcattgcat ctgcatcacc gcgttgcaca attttaaatg
    19801 cttctccgat tgagtttgta cctgttgcac atgctgtaac tgttgcacca tttggtcctt
    19861 ttgcacctaa gtcaattgat acttggccag ttgccatatc aggaattaac attggtacga
    19921 aaaatggact cacacgtctt gggcctttat ccattaattg tttatgtgca atttcaaatg
    19981 tttccatacc accgatacca gaaccaatcc atacaccgat tcgatctgca gtattttcat
    20041 tgatatctaa ttgcgcatct ttaacagcct ctctagctgc tacaattgca tattgagtaa
    20101 atctatccat acgacgcgct tcttttttgt cgatatgatc ttcaatatta aagtttttaa
    20161 gttctcctgc taagtgaacg ctataaggtt cagtatcgat acgtgtaatt ttatcgatac
    20221 catttacgcc ttttagagca ttctcccatg ttgttttgac atcattaccg attggagaaa
    20281 gggctcccat acctgtaata actactcttt tattttgact cattcgttat cctcctattt
    20341 tccccatttt attgtcattg cgccccaagt taggccgcca ccgaatccga caagaacaat
    20401 tgtatcatca tctttgagtt taccattttt taattcttga tcgatactta aaggtattga
    20461 cgcagctgaa gtatttccat atttatttac agaaacactc attttgtctt ttgaaatacc
    20521 taagcgttct ctagctgatt ccataattct aatattagct tgatgaggaa taaataaatc
    20581 tatatcatct gatgttaaat tcgctttttc aactacacgt gttgatgcat cacccataat
    20641 tctaacagca aatttaaata cttctcgacc attcattttc agtttaccag tatctttatc
    20701 taaatataaa tgtttgccac cagtgccatc agaacccatt tcataactta taatacctct
    20761 accttctgaa acttcaccga tgataaccgc acctgcacca tctccaaata gaactgcagt
    20821 agaacggtca gttaaatctg ttattttaga taatttatct gcaccgacaa ctaaaatatt
    20881 atgataatct ccagattgaa catattgttt agctgtaatc attgaataca taaatccaga
    20941 acatgctgca agttgatcca tagaggcaac tttgcccgtc cctaaacgtt cttgcaacat
    21001 atttgcgaca gttggaaatg gcatatctcc agttgctgtg gcaacaatta tcatatctat
    21061 atcttcgggc tgaataccag cgtcagcgat tgcttttaca cttgcttcat atgctaaatc
    21121 tgaagtatct tgatcgtcat ctgcccaatg tctttcttta attccagtca tcttagaaat
    21181 ccattcatca gatgtatcta aaaattgctc aaaataggca ttgtcaataa tcttttctgg
    21241 cgcatatgca ccaaaacctt taatacccac gttcatagtt gaacaccttc ttaataaaat
    21301 ttttaatacc aggtattaat ttaacataga ttacttttta aactcaagtc agagatagga
    21361 atattgacta gttaataaaa ttttaaagct ttattatcgt caaatttcag tatgaaattg
    21421 gttttaatgg gcttaaatta gaatagccat tcagataaaa tcatttattt aaactgtata
    21481 atatggtcaa aatatatgag atgttactat acaaatttct aactttcact taaaataaaa
    21541 tatatataat taaagtggag gtagaatgac atgaaacacc tcgtaacatt cttttgggca
    21601 ttattattaa tgcaaatggt taactttgta cttaatagcc tagtaggtgg agacagctta
    21661 aatgtagtga atccaattat catggcagta ttgttcacga ttttcacagc aatttttgct
    21721 gcagtaatta aaccgccgaa agattcatca caataactat ttagtataaa atgaaatatg
    21781 tctttcttat tattgaagct gcccagataa aggcggcttt tatttttggc cattaatgtt
    21841 ttgtattttg aatattttaa aactcaatga cctctatccc atcactttta aatatagaaa
    21901 aagccttaat acaaagatgt catttacgag taatctttgt atcaaggcaa ttatattata
    21961 atcatcactg taaaattaaa tgcttcatgt tgaatttgat aattttaagt gtttatttag
    22021 tttttacatt aatatcaatt tgtttaattg aatttgaatc aaacaagttt gctgtataac
    22081 tatctttctt taaatccact tcttttttgc tgccatcttt ccaagtaatc gtgtaaacgg
    22141 ctgattttgc atttcgaatc ttctcaggtg ttacaccacg ttcatcatat aataaagctt
    22201 taactttatt tcctaaatct aaatatgata attggctatc tttaggtaaa tttaagtaag
    22261 attgattgaa agccataatg ccatctactg caattgtata tggaacattt tgcatttctt
    22321 tagctttttg agttgccggt agtttgtcct tttctgctgc agatacctca ttacttacca
    22381 cagtagcgcc agtcgttgcg atcatgccca gtgccaaagt tacagttata aatgatttta
    22441 atttcatata atctctctcc tttgtgagtt aaagtatctt acaaaatcaa tataacacaa
    22501 atctaaaagt ttcaaaaatt ataagtatgt aaattgttta cttgttttta aaaataaatt
    22561 ttaattaatg taaaagctat aattgttaat actttctatt ttttcaaata aattcacatt
    22621 acaataacta agtttacatt tcttaaattt aattacttca ttgcattata gcgaacgccg
    22681 tacctaatat ataaattaaa tcccttgttt cttcgtactt ttaattgaac aattttatat
    22741 tgatgagata tttagtatta aatttttctt cctataaggt taatacttgt ttgatctatt
    22801 ttatgttgat ttacttcttt taaaaattca ttcaagtaga acccattctt caaatttaaa
    22861 gaatgatcta acgcataaac agttaattca tattgatgat ctttatcagg tggtgttggt
    22921 cctacatagt gattttctat ttctgaaaaa tcatcagcta acaaaccact agtgaagcta
    22981 tttttacctt gtacatagtc gccctttgtt cttgataaat ctgctttaat agaaattgaa
    23041 ttgccactaa cacttacatt agctacactc caatgaatcc aagcaaagcc acatactggg
    23101 attgcatcat agtcaattaa agaccaagca agatatttag tcccttccgg taattcagtt
    23161 acttcaaatg gaaatgattt aattggattc ccgttaatag aatcactcac atctgcacct
    23221 gctgcgtatt catatggtat attgttccct ttaaattcag tgttaatttt catgttgctc
    23281 tctccttgta aaatctttta ttcattcagc cttaacctct gatataatat taatattatt
    23341 tttatgcaat aaaaattaaa tattatctaa tgaatcatta acttttttga aagaggtaag
    23401 ttcatgaatt tggattggta ctatacattt gtcattttag cgaagacgtt aaactatcga
    23461 ttagctagtg aagaaattaa tctcactata ccttctatac ataagcaaat taaaaattta
    23521 gaacaacatc tcaatgtgaa actatttgaa acttataaaa atcaaattat cttgactgaa
    23581 gatggtcata catttcttcc aattgcgcaa agttttattg aacaatatga aagtggcatc
    23641 aaacatatcc aacttaaaaa aacgatgttt caatcgaaat taaatgttgt cgtgtcttca
    23701 tatatcgcga cattcattat gcccaaattt ttaaaatcat tctttaacga gcatcctttt
    23761 atcgatgttt ctcttcatgt caaaaatgaa aatattgaaa aagatattaa taatcatacg
    23821 tatgacatcg ggattagcag aaatcaacct aaactaagag aagttcattc tgaaaaagta
    23881 tgcgaaggta aaattgtatt gattgctccc aataaagaga ataatcatct tctaactgag
    23941 gcatctttat ttgaaaaata caaaataata agtgataacc atccagaata ttggtcatca
    24001 cttaaaaata atattttaaa tatttatgaa aaagcacaat ttctatctat caatgatgtg
    24061 catacttcaa ttaaattaat cgaaatgaat caaggtatat cattcctgcc tatttatata
    24121 acaaaaaata gtaattacaa tatttcggtt attaacacga aaattttaca agcacctatt
    24181 tcatttacat atatatacag caaaaaagaa aatccaga
//
LOCUS       DAPIDM010000028        22119 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000028 DAPIDM010000000
VERSION     DAPIDM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.028"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..309
                     /locus_tag="RJE42_000991"
     CDS             <1..309
                     /locus_tag="RJE42_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDV5876431.1"
                     /translation="ETQKRRLNQTPLHKYIKKIFISEETGYQKPNPEFFNYVFNDIGE
                     DERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDYEINSWKQLNDIVR
                     "
     gene            complement(417..857)
                     /locus_tag="RJE42_000992"
     CDS             complement(417..857)
                     /locus_tag="RJE42_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV5876432.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            1052..2665
                     /gene="bshC"
                     /locus_tag="RJE42_000993"
     CDS             1052..2665
                     /gene="bshC"
                     /locus_tag="RJE42_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDV5876433.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVIKGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFIYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQKRTQNQGLKAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYTKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFIGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASDQFIEKVEGMIEQQRRLNQDLLNEVVGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGIDVFKPSTYPPLSYTFDHIIIKP"
     gene            2809..3240
                     /gene="mraZ"
                     /locus_tag="RJE42_000994"
     CDS             2809..3240
                     /gene="mraZ"
                     /locus_tag="RJE42_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV5876434.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            3256..4191
                     /gene="rsmH"
                     /locus_tag="RJE42_000995"
     CDS             3256..4191
                     /gene="rsmH"
                     /locus_tag="RJE42_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV5876435.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            4205..4606
                     /gene="ftsL"
                     /locus_tag="RJE42_000996"
     CDS             4205..4606
                     /gene="ftsL"
                     /locus_tag="RJE42_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV5876436.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            4587..6821
                     /locus_tag="RJE42_000997"
     CDS             4587..6821
                     /locus_tag="RJE42_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDV5876437.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQLYKGQKQIAGKPITKETAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPNMTGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            7113..8078
                     /locus_tag="RJE42_000998"
     CDS             7113..8078
                     /locus_tag="RJE42_000998"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV5876438.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            8080..9429
                     /locus_tag="RJE42_000999"
     CDS             8080..9429
                     /locus_tag="RJE42_000999"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV5876439.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            9445..10764
                     /locus_tag="RJE42_001000"
     CDS             9445..10764
                     /locus_tag="RJE42_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDV5876440.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQAEFDEEIYLINKDFKKEESKDENNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDGQNESNGIAVDNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            10869..12275
                     /gene="ftsA"
                     /locus_tag="RJE42_001001"
     CDS             10869..12275
                     /gene="ftsA"
                     /locus_tag="RJE42_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV5876441.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            12308..13480
                     /gene="ftsZ"
                     /locus_tag="RJE42_001002"
     CDS             12308..13480
                     /gene="ftsZ"
                     /locus_tag="RJE42_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV5876442.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            13740..14531
                     /gene="pgeF"
                     /locus_tag="RJE42_001003"
     CDS             13740..14531
                     /gene="pgeF"
                     /locus_tag="RJE42_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDV5876443.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDNLTDEL
                     HGIDAMYTFDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKSKFETLPIDSGNYIETRGRDRHGIDLKKANAAL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            14555..15223
                     /locus_tag="RJE42_001004"
     CDS             14555..15223
                     /locus_tag="RJE42_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDV5876444.1"
                     /translation="MKDNLQQISTQINDKSKKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            15220..15783
                     /locus_tag="RJE42_001005"
     CDS             15220..15783
                     /locus_tag="RJE42_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDV5876445.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            15795..16085
                     /locus_tag="RJE42_001006"
     CDS             15795..16085
                     /locus_tag="RJE42_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDV5876446.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIFVLVLFQKGLLQIFNWILIQLQ"
     gene            16195..16974
                     /locus_tag="RJE42_001007"
     CDS             16195..16974
                     /locus_tag="RJE42_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDV5876447.1"
                     /translation="MINIDIYQHFRREEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFDLTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKIESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            16998..17615
                     /locus_tag="RJE42_001008"
     CDS             16998..17615
                     /locus_tag="RJE42_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDV5876448.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTSDNNQQSNDSETTKK"
     gene            17836..20589
                     /gene="ileS"
                     /locus_tag="RJE42_001009"
     CDS             17836..20589
                     /gene="ileS"
                     /locus_tag="RJE42_001009"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV5876449.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGYHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRHDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            20878..21675
                     /locus_tag="RJE42_001010"
     CDS             20878..21675
                     /locus_tag="RJE42_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5876450.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGLKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKDYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            21968..>22119
                     /locus_tag="RJE42_001011"
     CDS             21968..>22119
                     /locus_tag="RJE42_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV5876451.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHR"
BASE COUNT         8134 a         3048 c         4219 g         6718 t
ORIGIN      
        1 gaaacgcaaa agagaaggtt aaatcagacg ccattgcata aatatattaa aaagatattt
       61 atatctgagg aaacaggata tcaaaaacct aatccggaat tttttaatta tgtttttaat
      121 gatattggtg aggatgaaag acagcactcg attatagttg gagattcttt aacatctgac
      181 attctaggtg gaatcaatgc gggtatagct acttgctggt ttaattttag aggatttgat
      241 cataatccag gaattatacc tgattatgaa attaattcat ggaaacaact aaatgatatt
      301 gtacgttaag acaaataatt tgaaaatgat ttacttatat tagctaagaa agtaattgta
      361 aaaaagacac tacgaaaaga tgcattttca tgctcgtagt gtcgaatatt attatcttat
      421 ggtttccaca ataagacatc atgtccctct atatttttag cttccacatc tgtaaatcca
      481 tgatgtaaaa agaaatcttt agaatcattt ctgccaatgg ctttaattgg catattgaaa
      541 ctctttgcaa aatcaatcaa ttctgaagcg taccctctgt tttgatattt tggtaatact
      601 tctaacttcc ataataatat ataatcttca aaatctggga agtagatttc ttcgacatca
      661 ccttttttta gtaatgccat tctagctcct aattgatctc cgacaaatat gccataaaat
      721 ggtgaatctg aacttgcatc aatcatttga ccgttcaact cattgaccat gtataagtct
      781 ttgttgccaa acgctctaaa gttttcgaat aattcgtcag ttttgtaatt aatttcaaga
      841 cgtttgattt cactcatatt tatctacccc ttatttgttt actactatta ttatacatta
      901 aaccacttgt gtttttcatt gatttgaaaa tgtaaaacaa tgaatattat aaattttcca
      961 tgattaaatt gattttcaag ccgtaaaatt gtcaatcacc ggtatagact ttatactata
     1021 gtttgtaatc gcgtacttaa ggagaaaaaa catggactgt aaagtagtta gtttaaatga
     1081 aaaagatcag tttataccaa aaataaagag cagtgaccct gtaataaaag gattatttca
     1141 atatgatgca gctcaacaaa ctagttttga aaaaaggatg tctaaagaaa ataatggaag
     1201 agaagcggca ttagcgaatg ttattcgtga atatatgagt gatttaaagc tttctagtga
     1261 acaagaatta aacatacaac atttagctaa tggttcaaaa gttgtgattg gtggacaaca
     1321 agcagggctt ttcgggggac cattgtatac attccataaa atattttcaa tcattacttt
     1381 atctaaggaa ttaacggata cacataagca acaagtagta cccgtttttt ggattgcagg
     1441 agaagatcat gacttcgatg aagtgaatca tacatttatt tataacgaaa atcatggatc
     1501 gctgcataag gttaaatatc atacaatgga gatgccagag acgactgtct ctagatatta
     1561 tcctgataag gctgaattga aacaaacttt aaaaacgatg ttcattcata tgaaagaaac
     1621 tgttcataca caaggtctac ttgagatttg tgacagaatt attgaccaat atgactcgtg
     1681 gactgatatg tttaaagcac tactgcatga aacatttaaa gcatatggcg ttctatttat
     1741 agatgcgcag tttgagccgt taagaaaaat ggaagcgcct atgtttaaaa agattttgaa
     1801 aaaacatcag ttgcttgatg atgcttttag agcaacacaa aaacgtactc aaaatcaagg
     1861 cttgaaggcg atgatacaaa cagatacaaa tgttcactta ttcttacatg atgaaaatat
     1921 gcgtcaatta gtttcgtatg atggtaagca ttttaaatta aataaaacag ataagaccta
     1981 tacaaaggaa gaaattataa atatagcgga aaatcaacct gaattatttt ctaataatgt
     2041 agtgacaaga ccattaatgg aagaatggct atttaacacg gtggcattta ttggaggacc
     2101 gagtgaaatt aagtactggg ctgaactaaa agatgtattt gaactatttg atgttgaaat
     2161 gcctatcgtg atgccaaggc ttagaattac ttatttaaat gaccgtattg aaaaattact
     2221 ttcgaaatac aatattccat tagaaaaagt gttagtcgat ggtgttgaag gagaaaggag
     2281 taagtttatt agagaacaag catcagatca atttattgaa aaggtagaag gtatgataga
     2341 acaacagcgt cgtcttaacc aagacttatt aaatgaagtg gtggggaatc aaaataatat
     2401 taaccttgtg aataaaaata atgaaattca tatacaacag tatgattatt tgttaaaacg
     2461 ttatctttta aacattgaaa gagaaaacga catcagtatg aagcaattta gagaaattca
     2521 agaaacactc catccaatgg gaggattaca agaaagaata tggaatccac ttcaaatttt
     2581 gaatgatttt gggatagatg tgttcaagcc ctccacctat ccaccacttt cttacacttt
     2641 tgatcatatt attataaaac cttaatatac caagggttta gcccgattta tcataatgat
     2701 aaatcgggca tttttttgtt ttttaaaata aatttcacaa attttgtata aatagtggtg
     2761 gatagtgggg agatgtggta aattatatat aaggtgaggt gataaaaaat gttcatggga
     2821 gaatacgatc atcaattaga tacaaaagga cgtatgatta taccgtccaa gtttcgttat
     2881 gacttaaatg agcgttttat tatcacaaga ggccttgata aatgtttatt cggttacact
     2941 ctagacgaat ggcaacagat tgaagagaaa atgaaaacct tacctatgac aaaaaaagac
     3001 gcacgtaagt ttatgcgtat gttcttctct ggtgctgttg aagtagaact tgataagcaa
     3061 gggcgtatta acatccctca aaacttgagg aaatacgcta atttaactaa agaatgtaca
     3121 gtaatcggtg tttcaaatcg tattgagatt tgggatagag aaacttggaa tgatttctat
     3181 gaagaatctg aagaaagttt cgaagatatt gctgaagatt taatagattt tgatttttaa
     3241 aatggaggaa ttgaagtgtt tcatcatatc agcgttatgt taaacgaaac cattgattat
     3301 ttaaatgtaa aagaaaatgg tgtgtacatt gactgtacgc taggtggagc gggacatgcc
     3361 ctttatttac taaatcaatt aaatgacgac ggaagattaa tagcaatcga tcaagaccaa
     3421 actgcaattg ataatgctaa agaggtatta aaggatcatt tgcataaggt gacttttgtt
     3481 catagcaact tccgtgaatt aactcaaata ttaaaagact taaacattga aaaagtagat
     3541 ggaatctatt acgacttggg tgtttcaagc ccacaactcg acattccaga acgaggattc
     3601 agttatcacc atgacgcaac attagacatg cgtatggacc aaacacaaga actaacagca
     3661 tatgaaattg ttaacaattg gtcatatgaa gcgttagtga agatttttta tcgctatggc
     3721 gaggagaaat tttcaaaaca gatagctcga agaatcgaag cacatcgcga acaacaacca
     3781 ataacaacaa cattagaatt agttgacatt ataaaagaag gtattcctgc aaaagcaaga
     3841 agaaaaggcg gacatcctgc aaaacgagta tttcaagcac tacgaattgc agtaaacgat
     3901 gaattgtcag cttttgaaga ttcaatagaa caagcgattg aattagtgaa agtagacggc
     3961 aggatttcgg taatcacttt ccattcttta gaagatcgtt tatgtaaaca ggtgttccaa
     4021 gaatatgaaa aaggtccaga ggtaccaaga ggattaccag ttataccaga agcatataca
     4081 cctaagttaa agcgtgttaa tcgtaaaccg attaccgcta cagaagaaga tttagatgac
     4141 aataacagag cacgaagcgc gaaattacgt gtagctgaaa tacttaaata aggagcaatt
     4201 aataatggct gtagaaaaag tgtaccaacc atatgacgaa caagtttata atagtatacc
     4261 gaagcaacaa ccacaaacta agcccgaaaa gaagactgtt tcgagaaaag tggttgtaca
     4321 attaactaaa tttgaaaaag ttttatacat aactttgatt actgtaattg ctatgttaag
     4381 tatttatatg ctatctttaa aaatggatgc gtatgatacg cgaggaaaga ttgcagattt
     4441 agattataaa atagataaac aatcaagtga aaacagtgct ttacaatctg aaatcaaaaa
     4501 gaattcttct tatgaacgca tatacgaaaa ggctaagaaa caggggatga gccttgagaa
     4561 cgataatgta aaggtagtgc gtagtaatgg cgaagcaaaa aattaaaatt aaaaaaaata
     4621 aaataggggc agtcctactt gttggtttat tcggactgct cttttttata ttggttttaa
     4681 gaatttcata tatcatgatt actggacatt ctaatggtca agatttagtc atgaaggcaa
     4741 atgagaagta tttagttaag aatgcacaac aaccagaacg aggaaagata tatgatcgta
     4801 atggtaaagt gctagcagaa gatgtagaaa gatataaact tgttgctgta atagataaaa
     4861 aggcgagtgc caattctaaa aaacctaggc atgtagttga taaaaaagag actgcaaaga
     4921 aattatctac agtcattgat atgaagccag aggaaattga aaagagactt agtcaaaaga
     4981 aagccttcca aattgaattt ggacgcaaag gaacaaattt aacgtatcag gacaaattga
     5041 aaatagagaa aatgaatttg cctggtattt ctttattgcc tgaaacagaa cgattttatc
     5101 caaatggcaa ttttgcatca cacttaattg gtagagctca gaaaaatccg gatactggtg
     5161 aacttaaagg tgcgcttgga gttgaaaaga tttttgatag ttatttaagt ggatctaaag
     5221 gatctttgag atatattcat gatatttggg gatatattgc accaaatact aaaaaagaga
     5281 agcagcctaa acgtggtgat gatgtccatt taacaatcga ttcaaatatt caagtatttg
     5341 ttgaagaggc tttagatggc atggttgaaa gataccagcc gaaagattta tttgcggttg
     5401 tcatggatgc caaaactgga gaaattttag catacagtca gcgaccaaca tttaatcctg
     5461 aaactggtaa agattttggt aaaaagtggg caaatgatct atatcaaaac acatatgagc
     5521 ctggatcaac atttaaatca tatgggttag cagctgctat tcaagaaggt gcttttgatc
     5581 ctgataagaa atataaatct ggacatagag atattatggg ttcacgtatt tcagactgga
     5641 atagagtcgg ttggggtgaa atcccaatgt cactcggatt tacttattca tctaatacat
     5701 tgatgatgca tttacaagat ttagttggtg cagacaaaat gaaatcttgg tatgaacgat
     5761 ttggatttgg aaaatcaact aaaggtatgt ttgatggaga agcacctggt caaattggat
     5821 ggagtaatga gttgcaacaa aaaacgtcat catttggtca atcgacaact gtaacacctg
     5881 ttcaaatgtt acaagcgcaa tcagcgttct ttaatgatgg taatatgtta aaaccatggt
     5941 ttgtgaatag cgttgaaaat cctgttagta aaagacaact ttataaaggt caaaaacaaa
     6001 tcgcaggcaa accaataaca aaagagactg ctgaaaaagt tgaaaagcaa ttggatttag
     6061 ttgtgaatag taagaagagt cacgctgcaa actatcgtat tgatggttat gaggtcgaag
     6121 gtaagactgg tacagcacaa gtcgctgcac ctaatggtgg tggatacgtt aaaggtccaa
     6181 acccatattt tgtaagtttt atgggtgacg caccgaagaa aaatcctaaa gttattgtat
     6241 acgctggtat gagcttggca caaaaaaatg accaagaagc ttatgaatta ggtgttagta
     6301 aagcgtttaa accaataatg gaaaatactt tgaaatattt aaatgtaggt aaatcaaaag
     6361 atgacacatc taatgcagag tatagtaaag tgccagatgt tgaaggtcaa gacaaacaaa
     6421 aagctattga taatgtgagt gcaaaatcat tagaaccagt tactatcggt tctggtacac
     6481 aaataaaagc acaatctata aaagcgggga ataaagtctt acctcatagt aaagtactgt
     6541 tattaacaga tggagactta actatgccta acatgacagg atggacgaaa gaagatgtca
     6601 ttgcttttga aaacctaaca aatattaaag tgaatttaaa aggtagcggt tttgtgtccc
     6661 accaatcaat tagtaaggga caaaaactta ctgaaaaaga taaaatagac gtagaatttt
     6721 catcagagaa tgtagacagc aattcgacga ataattctga ttcaaattca gatgataaga
     6781 agaaatctga cagtaaaact gacaaggata agtcggacta acaagaatgg ctacattaaa
     6841 taaggatgta ttgtcactat ttgtttttac taaagtaatg ctgtgataga aaacaacatc
     6901 ttcatttaat ctttataaat aagtgttgat agagaatttt aaggtagacg aggtcagatt
     6961 aacttgtttg ttataggcaa actttttata ctggcctctt atgccgtaaa aaagaaaaat
     7021 tataaatttt gattacaact ttgtttattt aattgaaaaa ttgataatat attagtggtt
     7081 aaaaaagtct ggattaagaa gaggagatag ttatgatttt tgtatatgcg ttattagcgc
     7141 tagtgattac atttgttttg gtacctgttt taatacctac attaaaaagg atgaaatttg
     7201 gtcaaagtat tcgagaagaa ggtccacaaa gccatatgaa gaagactggt acaccaacaa
     7261 tgggtggact aacatttcta ttaagtattg tgataacgtc tttggtggct attatatttg
     7321 tagatcaagc taatccaatc atactgttat tatttgttac gattggtttt gggttaattg
     7381 gttttataga tgattatatt attgttgtta aaaagaataa ccaaggttta acaagtaaac
     7441 agaagttttt ggcgcaaatt ggtattgcga ttattttctt tgttttaagt aatgtatttc
     7501 atttggtgaa tttttctacg agcatacata ttccatttac gaatgtagca atcccactat
     7561 catttgcata tgttattttc attgtttttt ggcaagtagg tttttctaat gcggtaaatt
     7621 taacagatgg tttagatgga ttagcaactg ggctgtcaat tatcggattt acaatgtatg
     7681 ccatcatgag ctttgtgtta ggagaaacgg caattggcat tttctgtatc attatgttgt
     7741 ttgcactttt aggattttta ccatataaca ttaaccctgc taaagtgttt atgggagata
     7801 caggtagctt agctttaggt ggtatatttg ctacgatttc aatcatgctt aatcaggaat
     7861 tatcattaat ttttataggt ttagtattcg taattgaaac attatctgtt atgttacaag
     7921 tcgctagctt taaattgact ggaaagcgta tatttaaaat gagtccgatt catcatcatt
     7981 ttgaattgat aggttggagc gaatggaaag tagttacagt attttgggct gttggtctga
     8041 tttcaggttt aatcggttta tggattggag tgcattaaga tgcttaatta cacagggtta
     8101 gaaaataaaa atgtattagt cgtcggtttg gcaaaaagtg gttatgaagc agctaaatta
     8161 ttaagtaaat taggtgctaa tgtgactgtc aatgatggga aagacttatc gcaagatgct
     8221 catgcaaaag atttagaatc tatgggcatt tctgttgtaa gtggaagtca tccattaaca
     8281 ttgcttgata ataatccaat aattgttaaa aatcctggaa taccttatac agtatctatt
     8341 attgatgaag cagtgaaacg aggtttgaaa attttaacag aagttgagtt aagttatcta
     8401 atctctgaag caccaattat agctgtaaca ggtacaaatg gtaaaacgac agttacttct
     8461 ctaattggag atatgtttaa aaaaagtcgc ttaactggaa gattatccgg caatattggt
     8521 tatgttgcat ctaaagtagc acaagaagta aagccaacag attatttagt tacagagttg
     8581 tcgtcattcc agttacttgg aatcgaaaag tataaaccac acattgctat aattactaac
     8641 atttattcag cgcatctaga ttaccatgaa aatttagaaa actatcaaaa tgctaaaaag
     8701 caaatatata aaaatcaaac ggaagaagat tatttgattt gtaattatca tcaaagacaa
     8761 gtgatagagt cggaagaatt aaaagctaag acattgtatt tctcaacaca acaagaagtt
     8821 gatggtattt atattaaaga tggttttatc gtttataaag gtgttcgtat tattaacact
     8881 gaagatttag tattgcctgg tgaacataat ttagaaaata tattagcagc tgtgcttgct
     8941 tgtattttag ctggtgtacc tattaaagca attattgata gtttaactac attttcagga
     9001 atagagcata gattgcaata tgttggtact aatagaacta ataaatatta taatgattcc
     9061 aaagcaacaa acacgttagc aacacagttt gccttaaatt catttaatca accaatcatt
     9121 tggttatgtg gtggtttgga tcgagggaat gaatttgacg aactcattcc ttatatggaa
     9181 aatgttcgcg cgatggttgt attcggacaa acgaaagcta agtttgctaa actaggtaat
     9241 agtcaaggga aatcggtcat tgaagcgaac aatgtcgaag acgctgttga taaagtacaa
     9301 gatattatag aaccaaatga tgttgtatta ttgtcacctg cttgtgcaag ttgggatcaa
     9361 tatagtactt ttgaagagcg tggagagaaa tttattgaaa gattccgtgc ccatttacca
     9421 tcttattaaa gggtgtgagt attgatggat gataaaacga agaacgatca acaagaatca
     9481 aatgaagata aagatgaatt agaattattt acgaggaata catctaagaa aagacggcaa
     9541 agaaaaagat caaaggctac acatttttct aatcaaaata aagatgatac atctcaacaa
     9601 gctgagtttg atgaagaaat ttacttgata aataaagact tcaaaaaaga agaaagcaaa
     9661 gatgaaaata atgattctgc ttctagtcat gcgaatgata ataatatcga tgattctaca
     9721 gactctaata ttgaaaatga ggattataga tataatcaag aaattgacgg ccaaaatgaa
     9781 tcgaatggaa ttgcagtcga caacgaacaa cctcaatcag ctcctaaaga acaaaatagt
     9841 gactcgaatg atgaggaaac agtaacgaaa aaagaacgaa aaagtaaagt aacacaatta
     9901 aagccattaa cacttgaaga aaagcggaag ttaagacgta agcgacaaaa acgaatccaa
     9961 tacagtgtta ttacaatatt ggtattgttg attgctgtta tattaattta catgttttca
    10021 ccacttagta aaattgcgca tgtaaatata aatggaaata atcacgttag tacttcaaag
    10081 ataaacaaag ttttaggtgt taaaaatgat tcgaggatgt atacgtttag taaaaaaaat
    10141 gctattaatg atctcgaaga ggatccatta atcaaaagtg ttgagataca caagcaatta
    10201 ccaaacacat taaacgtaga tatcacagaa aatgaaatta ttgctttagt gaaatataaa
    10261 ggtaaatatt tacctttatt agaaaatggt aaattgctta aaggttcaaa tgatgtcaaa
    10321 attaatgatg cacctgtcat ggatggtttc aaaggtacaa aagaagatga tatgattaag
    10381 gcgttatctg aaatgacacc tgaagttaga cgatatattg ccgaagtgac atacgcccca
    10441 agtaaaaaca aacaaagcag aattgaattg tttacgacgg atggacttca agtaatcggt
    10501 gatatttcga cgatatctaa gaaaatgaaa tattatccgc agatgtcaca atcattatca
    10561 agggatagtt cgggtaaact aaaaacacga ggctatattg atttatcagt cggtgcttca
    10621 tttatcccat accgtggaaa cacgtctagt caatcagaaa gcgataaaaa tgtgactaaa
    10681 tcatctcaag aggaaaatca agcaaaagaa gaattacaaa gcgttttaaa caaaattaac
    10741 aaacaatcaa gtaagaataa ttaaaaaatt ttgatattgt ctatgtttat agttcacaag
    10801 ccattcaacg tattgtaaac taaagatagt gtatttttta atagtaattt gtcaggaggt
    10861 gcctatctat ggaagaacat tactacgtaa gtattgatat tggatcatca agcgtaaaaa
    10921 caatagtagg cgagaaattt cacaatggta taaatgtgat aggtacagga caaacctaca
    10981 cgagcggtat aaaaaatggt ttaattgatg attttgatat tgcgcgtcaa gcaatcaaag
    11041 acacaattaa aaaggcatca atcgcttcgg gtgttgatat taaagaagtt ttcctgaaat
    11101 tacctatcat tggaacggaa gtttatgatg aatcaaatga aatcgacttt tatgaggata
    11161 cagaaatcaa cggttcacat atcgaaaaag tattagaagg tattagagaa aaaaatgatg
    11221 tgcaagaaac agaagtaatt aatgtgttcc cgattcgttt tatagtcgat aaagaaaatg
    11281 aggtttcaga ccctaaagaa ttaattgcca gacattcatt aaaggttgaa gcaggcgtaa
    11341 ttgctattca aaaatcgatt ttaattaata tgattaaatg cgttgaagca tgtggtgttg
    11401 atgtattaga tgtttactct gatgcatata actatggttc aatcctaaca gctactgaaa
    11461 aagagttagg tgcatgtgtc attgatattg gtgaagacgt tacgcaagtt gctttttatg
    11521 aacgcggtga attagtagat gctgattcta tcgaaatggc agggcgtgat attacagacg
    11581 atattgcaca aggattaaac acttcttatg aaactgctga aaaagttaag caccaatatg
    11641 gtcatgcatt ctatgattct gcttcagatc aagatatctt cactgttgaa caggttgata
    11701 gtgatgaaac agtacagtat actcaaaaag atttgagtga ctttattgaa gcgcgtgtag
    11761 aagaaatatt cttcgaagta tttgatgttt tacaagattt aggattaaca aaagtaaatg
    11821 gtgggtttat tgtaactggt ggatctgcaa acttacttgg cgtaaaagaa ttattatcag
    11881 atatggtaag tgaaaaagtt agaattcaca cgccatcaca aatgggaatt agaaaacctg
    11941 aattttcttc agcaatttct acaatttcta gtagtatcgc ttttgatgag ttattagatt
    12001 atgttacaat taattatcat gataatgaag aaactgaaga agatgttatt gatgtgaaag
    12061 acaaagataa cgaatctaaa ttaggcggat ttgattggtt taaacgtaaa acaaacaaaa
    12121 aagatactca tgaaaatgaa gtagagtcaa cagatgaaga aatttatcaa tcagaagata
    12181 atcatcagga acataaacag aatcatgaac atgttcaaga caaagataaa gaagaaagta
    12241 aattcaaaaa actaatgaaa tctctatttg aatgattatt ggccaataaa actaggagga
    12301 aatttaaatg ttagaatttg aacaaggatt taatcattta gcgactttaa aggtcattgg
    12361 tgtaggtggt ggcggtaaca acgccgtaaa ccgaatgatt gaccatggaa tgaataatgt
    12421 tgaatttatc gctatcaaca cagacggtca agctttaaac ttatctaaag ctgaatctaa
    12481 aatccaaatc ggtgaaaaat taacacgtgg tttaggagca ggagctaatc ctgaaatcgg
    12541 taaaaaagct gcagaggaat ctcgtgaaca aattgaagat gcaatccaag gtgcagacat
    12601 ggtatttgtt acttctggta tgggtggcgg aactggtact ggtgcagcac cagtcgttgc
    12661 taaaattgca aaagaaatgg gcgcattaac tgttggtgtt gtaactcgtc catttagttt
    12721 tgaaggacgt aaacgtcaaa ctcaagctgc tgctggagta gaagctatga aagctgcagt
    12781 agatacatta atcgttatac caaatgaccg tttattagat atcgttgaca aatctacgcc
    12841 aatgatggaa gcatttaaag aagctgacaa cgtgttacgc caaggtgtac aaggtatctc
    12901 agacttaatc gctgtttctg gtgaagtaaa cttagacttt gcagacgtta agacaattat
    12961 gtctaaccaa ggttctgcat taatgggtat tggtgtttct tctggtgaaa atagagcggt
    13021 agaagctgct aaaaaagcaa tctcttctcc attacttgaa acatctatcg ttggtgcaca
    13081 aggtgtgctt atgaatatta ctggtggcga gtcattgtca ttatttgaag cacaagaggc
    13141 tgctgatatt gtccaagatg ctgcagatga agacgttaat atgattttcg gtacagttat
    13201 taatcctgaa ttacaagatg agattgttgt aacagttatt gcaactggtt ttgatgacaa
    13261 accaacatca catggtcgta aatctggtag cactggattc ggaacaagcg taaatacttc
    13321 tagcaatgca acttctaaag atgaatcatt cacttcaaat tcatcaaatg cacaagcaac
    13381 tgatagtgta agtgaaagaa cacatacaac taaagaagat gatattccta gcttcattag
    13441 aaatagagaa gaaagacgtt caagaagaac aagacgttaa tcggttaata tatatacaca
    13501 aataattcaa cacaaatcat cagataacat atctgatgat ttttttacta atttttagaa
    13561 catgtagaag gacatttaag tttttcgaag tcattaaaag tgtttaagta tcgtgtcaaa
    13621 attaagtcga aaattatttg cgcaacattt taactttaaa cataaatgtt atattatata
    13681 attattaact ttgtacagtt agacgaagat aatttaaatg aaatgatggt gacgatcgag
    13741 tgaatgataa ttttaaaaag caaccgcatc atttaatata tgaagaatta ttacaacaag
    13801 gtattactct aggtattaca actagaggcg atggtttaag tgattatcct aaaaatgctt
    13861 ttaatatggc gagatatatt gatgatcgcc catataatat tactcaacat caattgcaat
    13921 tagctgaaga aattgcgttt gatagaaaaa attgggtgtt tcccattcaa acacatgaaa
    13981 ataaagtcgc ttgtattaca aaggatgata taggcacaaa tatagacaat ttaactgatg
    14041 agcttcatgg tattgatgcg atgtacacat ttgatagtaa tgtcttatta acgatgtgtt
    14101 atgcagactg tgtaccagta tatttttata gtacaaaaca tcattttatt gcattggcgc
    14161 atgcagggtg gcgaggtacc tacactgaaa ttgtaaaaga agtgctaaaa catgtgaact
    14221 ttgatttgaa agacttacat gtcgttattg gaccatctac atcatcaagt tatgaaatta
    14281 atgatgatat taaaagtaaa tttgaaacat tgccaattga tagtggaaac tatattgaaa
    14341 ctagaggacg agatcgtcat ggtattgatt tgaaaaaagc caatgctgca ttattaaatt
    14401 attatggtgt tcctaaagaa aatatttata cgacagcgta tgctacatct gaacatttag
    14461 aattattttt ctcttatcga ttagaaaaag gtcaaacagg acgcatgtta gcattcattg
    14521 gtcaacagta aataaggagg agatatgctt gcgtgtgaaa gataatttac aacaaatctc
    14581 aacacaaatt aatgacaaaa gtaaaaaaaa taatttttca acaaaaccaa acgtgattgc
    14641 agttacaaaa tatgttacaa tagagcgagc taaagaagcg tatgaggctg gaataagaca
    14701 ttttggtgag aatagattgg aaggcttttt tcaaaagaaa gaagcattac catcagatgc
    14761 ggtgatccat tttatagggt cattacaatc tcgaaaagtt aaggacgtta taaacgacgt
    14821 agattatttc catgctttag atcgattgag cttagccaaa gaaattaaca aacgtgcaga
    14881 acataaaatt aaatgtttct tgcaagtgaa cgtttcggga gaagcttcta aacatggtat
    14941 tgctttagaa gatgttgatc agtttataga tgatcttaaa aaatatgaca aaatcgaaat
    15001 tgtaggttta atgacgatgg caccattgac agatgatgaa gcatatatta gatcgttatt
    15061 taaacagtta cgtttgaaaa aagaagaaat acaacgactc aatttagaat atgcgccttg
    15121 tgatgaatta tcaatgggaa tgagtaatga ctatcttatt gcagttgaag aaggtgcaac
    15181 gtttgttaga attgggacta aacttgtagg agaagaggag tgagccactt ggctttaaaa
    15241 gatttattta gtggattttt tgtaatagat gatgaagagg aagtagaagt acctgacaaa
    15301 caacaacagg taaatgaagc gccagcaaaa gagcagtcac aacaaacaac aaaacaaaac
    15361 gcaatcaaat cagtccctca aaaatctgca tcaagatata caacaacgtc agaagaaagg
    15421 aataaccgta tgtctaatta ttcaaaaaat aattcacgta atgttgtaac tatgaacaat
    15481 gctacaccaa acaatgcatc acaagaaagt tcaaaaatgt gtttattcga accacgtgtt
    15541 ttttcagata cacaagatat tgctgatgag cttaaaaacc gccgtgcgac acttgtcaat
    15601 ttacaacgta ttgataaagt atcagcgaaa agaattattg attttttaag cggtactgtt
    15661 tatgcaatcg gtggagatat ccaacgtgta ggtactgata ttttcttatg tacgcctgat
    15721 aatgtggaag tagctggaag cattacagac catattgaaa atatggaaca ttcattcgac
    15781 taaggagtta acatatggat ataaatgtgc tagctacaat atttaaattt atcctttttg
    15841 ttgttgaaat ttattatttc ggcatgatta tatatttctt tacatcttgg gtaccaagta
    15901 ttagagaaac taaggtgggt tattttttag cgaaaatata tgaacctttc ttacaaccat
    15961 ttagaaaagt aattccacct attggaatta tcgacatatc atcaatcgct gcaattttcg
    16021 ttttagtatt attccaaaaa gggttactcc aaatctttaa ttggatttta attcaattac
    16081 aataaattaa gcgacataac gaggcaaggg tacatgatac tttagcctcg tttttgatat
    16141 gtgtttttct gaatataagg gcaatagatg gtattttata atttttttaa ggtagtgatt
    16201 aacatagata tttatcaaca ctttagacgg gaagaatacg aattaattga tcagctaacg
    16261 gataaatgtg atcaagcgga acagcattat gcaccagtat taacgcattt tttagatcca
    16321 agagggcaat atatattgga agtgatttgt ggcagttatg aagatttaaa cgtatctttt
    16381 tatggcgggc caaatgctga aagaaaaaga gcaatcattt cgccgaacta ttatgaacct
    16441 aaagaaagtg actttgattt aactttaatg gaaatagatt atcctgaaaa attcgtcact
    16501 ttaaaacatc aacatatttt agggacatta atgtctttag gtatcgaacg cgaacaagtt
    16561 ggagatataa ttgtgaatga acgaattcaa tttgttttga caagtagatt ggaatcattt
    16621 attatgttag aattacaacg tattaaaggc gcatcagtta aactttatac tattccagta
    16681 acagatatga tacaatctaa tgagaattgg aaaattgaaa gtgcaacagt tagttcttta
    16741 aggttagatg ttgttattaa agaaatgata cgtaaatcac gtacgattgc gaaacaacta
    16801 atcgaaaaaa aacgtgttaa agtgaatcac actattgttg attcagcaga ttttcaatta
    16861 caagcaaatg atttaatatc catccaaggt tttggtagag cacacattac tgacttaggt
    16921 ggtaaaacta aaaaagataa aacgcacatt acctatagaa cattattcaa atagtaatga
    16981 tttaaggagg ataacaaatg ccttttacac caaatgaaat taagaataaa gagttttcac
    17041 gtgtaaagaa tggtttagaa cctactgaag ttgctaattt tttggagcaa ctaagcactg
    17101 aaattgaacg tcttaaagaa gataaaaaac aacttgaaaa agtaatcgaa gagagagata
    17161 ctaatattaa gtcttatcaa gacgtgcatc aatctgtaag tgatgctttg atacaagctc
    17221 aaaaagctgg tgaagaaact aagcaagctg cagagaaaca agctgaagcg attatagcta
    17281 aggcagaagc gcaagctaat caaatggttg gtgacgcggt agaaaaagca cgccgtttag
    17341 cattccagac tgaagatatg aaacgtcaat caaaagtatt tagatcgcgt ttccgtatgt
    17401 tagttgaagc gcaattagac ttattaaaaa acgaagattg ggattacttg ttgaattatg
    17461 atttagacgc tgaacaagtg acgcttgaaa atattcatca tttgcatgaa aatgatttaa
    17521 agccagatga agttgcagca aatgcacaaa ataatgcatc gaatacatca gacaataatc
    17581 aacaatccaa tgattcagaa acaactaaga agtaagaatt aaataaagac agacgcgtaa
    17641 tatacattta acttttcaca gcgaattagg taatggtgag agcctagtaa aagcatgtat
    17701 gttatatcac tggcttttta atatttaaat aatgtaatga gagaactcta agttgagtta
    17761 ataagggtgg taccgcgagc aatcgtccct tttaatttaa cttagagttt tttaaatttt
    17821 taaggagtga agaagatgga ttacaaagaa acgttattaa tgcctaaaac agatttccca
    17881 atgcgaggtg gtttaccaaa caaggaaccg caaattcaag aaaaatggga tgcagaagat
    17941 caataccata aagcgttaga aaaaaataaa ggtaacgaaa cattcatttt acatgatggc
    18001 ccaccatacg cgaatggtaa cttacatatg ggacatgcct tgaacaaaat tttaaaagac
    18061 tttattgtac gttataaaac tatgcaaggg ttctatgcac cgtacgtacc aggttgggat
    18121 acacatggtt taccaattga acaagcatta acgaaaaaag gtgttgaccg taagaaaatg
    18181 tcaacagctg aattccgtga gaaatgtaaa gaatttgctt tagaacaaat tgaattacag
    18241 aaaaaagatt ttagacgttt aggtgttcgt ggtgacttta atgatccata tattacatta
    18301 aaacctgaat acgaagctgc acaaattcgt atttttggag aaatggcaga taaaggttta
    18361 atttacaaag gtaaaaagcc agtttattgg tctccttcaa gtgagtcttc attagcagaa
    18421 gcagaaattg aatatcacga taaacgttca gcatcaattt acgttgcatt tgacgttaaa
    18481 gatgacaaag gtgtcgttga tgcagatgct aaatttatta tctggacaac aacgccatgg
    18541 acaattccat caaatgttgc gattaccgtt catcctgaat taaaatatgg tcaatacaat
    18601 gtaaatggcg aaaaatatat tattgcagaa gccttatctg acgccgtagc agaagcactg
    18661 gattgggata aagcatcaat caaattagaa aaagaataca caggtaagga attggagtat
    18721 gttgtagcac aacatccatt cttagataga gaatcgttag tgattaatgg ttatcatgtt
    18781 actacagatg ctggtacagg ttgtgtacat acagcaccag gtcacgggga agatgactat
    18841 attgttggtc aaaaatatga attaccagta attagtccaa tcgatgataa aggtgtattt
    18901 actgaagaag gcggccaatt tgaaggaatg ttctatgata aagctaataa agccgttact
    18961 gatttattaa cagaaaaagg tgcactatta aaattagact ttattacaca tagctatcca
    19021 cacgactgga gaacaaaaaa acctgtaatc ttccgtgcta caccacaatg gtttgcctca
    19081 atcagtaaag taagacatga tattttagat gcaatcgaaa atacaaactt caaagtaaat
    19141 tggggtaaaa cacgtattta caatatggtt cgtgaccgtg gcgaatgggt tatttctcgt
    19201 caacgtgttt ggggtgtacc gttaccagta ttttatgctg aaaatggcga aattatcatg
    19261 acgaaagaaa cagtgaatca tgttgctgat ttatttgcag aacacggttc aaatatttgg
    19321 tttgaaagag aagcgaaaga cttactacca gaaggattta cacatccagg cagccctaac
    19381 ggtacattta ctaaagaaac agacattatg gacgtttggt ttgattctgg ttcatcacac
    19441 cgtggcgtgt tggaaacaag accggaatta agtttcccag cggatatgta tttagaaggt
    19501 agtgaccaat atcgtggttg gttcaactct tctattacaa cttcagttgc tacaagagga
    19561 gtatcacctt ataaattctt actttctcat ggttttgtta tggacggtga aggtaagaaa
    19621 atgagtaaat ctttaggtaa tgtgattgta cctgaccaag tggttaaaca aaaaggtgct
    19681 gatattgcaa gactttgggt aagtagtacg gactatttag ctgatgttag aatttctgat
    19741 gaaattttaa aacaaacatc tgatgtttat cgtaaaatca gaaatacatt aagatttatg
    19801 ttaggtaata ttaatgattt caatcctgac acagatagca ttcctgaatc agagttatta
    19861 gaagtggatc gttacttgct aaatcgttta cgtgaattta ctgcaagtac gattaacaac
    19921 tatgaaaact ttgactactt aaatatttat caagaagttc aaaactttat caatgttgag
    19981 ttaagtaatt tctatttgga ttacggtaaa gatattttat atattgaaca acgtgattct
    20041 catatccgtc gtagtatgca aacagtgtta tatcaaattt tagttgatat gacgaagttg
    20101 ttagcaccaa tcttagtgca tacagctgaa gaagtttggt ctcatacacc acatgttaaa
    20161 gaagaaagtg ttcacttagc agacatgcct aaagttgtag aagtagatca agctttattg
    20221 gataaatggc gtacatttat gaatttacgt gatgatgtga accgtgcatt agaaactgct
    20281 cgtaatgaaa aagttattgg taaatcatta gaagctaaag ttacgattgc tagtaacgat
    20341 aaatttaatg catctgaatt cttaacttca tttgatgcat tacatcaatt atttatcgtg
    20401 tcacaagtta aagttgtaga taagttagat gatcaggcaa cagcttatga acatggtgat
    20461 attgtcatcg aacatgcaga tggtgaaaaa tgtgaaagat gttggaacta ttcagaggat
    20521 cttggtgctg ttgatgaatt gacgcatcta tgcccacgat gccaacaagt tgtaaaatca
    20581 cttgtataat tgaaattgta taaagtactc atacagatga tatagattaa agctctcttc
    20641 ataatcatgt tgtagttttt gttgacatga tgaagagagt ttttttgtga aaaaaaatga
    20701 ccaagttacc ggtcatatgt gtaaaaaaat gtgcgattta ctaaaataaa aattattcag
    20761 gaatggtaca aattctctga ggcttataaa tgcgttatag ttgctattct caattatgtt
    20821 cgcgataatt ttaagtaaaa gtaagcacag atattgaatt tgataggagt taattgaatg
    20881 tatcataaca gtaacgcaaa ctttgtaaat ggtatcactc taaatgtgag agataagaat
    20941 gaattaaagc cattttatga ggacatatta ggattaaata ttataaatga gacattaaca
    21001 tcgatacaat atgaagtagg tcaaaataat catgtcatta cacttgttga attacaaaat
    21061 ggacgtgaac ctttaatgtc cgaagcggga ctgtttcata tcgcaattaa actacctcaa
    21121 attagtgatt tagctaattt actaattcat ttaagcgaat atgatattcc agttaacgga
    21181 ggtatacagc ctgcttcgtt atcattgttt tttgaagacc cggaaggaaa cggtttaaaa
    21241 ttttatgttg ataaagacga agcgcaatgg acgaggcaaa atgatttagt aaaaattgat
    21301 attagaccat taaatgtacc gagattagtg agtcatgcaa caaaattgtt atggttaggt
    21361 attccagatg acgctattat aggtgcattg catattaaga caattcattt atcagaggta
    21421 aaagactact acctcgatta ttttggatta gagcaatcgg catatatgga tgattattca
    21481 atatttttag catcgaatgg ctattatcaa catttggcca tgaatgattg ggtatcagca
    21541 acgaaacgtg tagaaaattt tgatacgtat ggattagcaa ttgttgactt tcattatcct
    21601 gaaacaacac atttaaattt acaaggtccg gatggtatct attatcgctt taatcatatc
    21661 gaagttgaag attagtatat actttgaatg gacgaaccat ataatgaatc gtttttaatg
    21721 atctttttat acaagctatg aaggcagtct tatgaggttt cccatgaggc tgctttccta
    21781 atttttaatg tcatcttaaa tatgataaat gaataattaa gttcatattt aatgtcaaaa
    21841 catagtagtt tatcaagtat tgagttagta acactagatt taatgtaata tgcttacttt
    21901 ttttattagc aggtgtaagc tataatataa agagttgtct tatggacgat tgattggagg
    21961 aacgaaaatg cacaaaaaat attttattgg cacttccatt ttaatagcag tatttgtcgt
    22021 tatatttgac caagttacta aatatattat agctactaca atgaaaattg gagattcatt
    22081 tgaagtgata ccgcactttt taaacataac atcacatcg
//
LOCUS       DAPIDM010000029        21530 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000029 DAPIDM010000000
VERSION     DAPIDM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.029"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            131..2227
                     /gene="pnp"
                     /locus_tag="RJE42_001012"
     CDS             131..2227
                     /gene="pnp"
                     /locus_tag="RJE42_001012"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV5876452.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2463..4136
                     /locus_tag="RJE42_001013"
     CDS             2463..4136
                     /locus_tag="RJE42_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDV5876453.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4392..6761
                     /locus_tag="RJE42_001014"
     CDS             4392..6761
                     /locus_tag="RJE42_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV5876454.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLR
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLIEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6766..7479
                     /locus_tag="RJE42_001015"
     CDS             6766..7479
                     /locus_tag="RJE42_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5876455.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYSVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7510..8775
                     /locus_tag="RJE42_001016"
     CDS             7510..8775
                     /locus_tag="RJE42_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV5876456.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITGRSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKNTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSVIEIMHNQILLEQPQSKETFIKDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8775..10061
                     /locus_tag="RJE42_001017"
     CDS             8775..10061
                     /locus_tag="RJE42_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV5876457.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPIGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPRYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPETICRIVK
                     QHEDARNKVNQPKIDRGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELCDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10061..10765
                     /locus_tag="RJE42_001018"
     CDS             10061..10765
                     /locus_tag="RJE42_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV5876458.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVHFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10870..11697
                     /locus_tag="RJE42_001019"
     CDS             10870..11697
                     /locus_tag="RJE42_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDV5876459.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11716..12108
                     /locus_tag="RJE42_001020"
     CDS             11716..12108
                     /locus_tag="RJE42_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5876460.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12142..12720
                     /gene="pgsA"
                     /locus_tag="RJE42_001021"
     CDS             12142..12720
                     /gene="pgsA"
                     /locus_tag="RJE42_001021"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV5876461.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12945..14096
                     /locus_tag="RJE42_001022"
     CDS             12945..14096
                     /locus_tag="RJE42_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDV5876462.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETVLIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKINEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNLEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            14261..15304
                     /gene="recA"
                     /locus_tag="RJE42_001023"
     CDS             14261..15304
                     /gene="recA"
                     /locus_tag="RJE42_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV5876463.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            15658..17217
                     /gene="rny"
                     /locus_tag="RJE42_001024"
     CDS             15658..17217
                     /gene="rny"
                     /locus_tag="RJE42_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDV5876464.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(17514..17729)
                     /locus_tag="RJE42_001025"
     CDS             complement(17514..17729)
                     /locus_tag="RJE42_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876465.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            17903..18700
                     /locus_tag="RJE42_001026"
     CDS             17903..18700
                     /locus_tag="RJE42_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDV5876466.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            18840..20600
                     /locus_tag="RJE42_001027"
     CDS             18840..20600
                     /locus_tag="RJE42_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDV5876467.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            20601..21467
                     /locus_tag="RJE42_001028"
     CDS             20601..21467
                     /locus_tag="RJE42_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDV5876468.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
BASE COUNT         7847 a         2941 c         4142 g         6600 t
ORIGIN      
        1 aagtctattt tgtaaataat agatggatat cccgctctct ttttttccaa ttcaatattt
       61 tataactaat attaaaatac gataataaat gatatgatat aactattaga ttcaagagag
      121 gagatttata atgtctcaag aaaagaaagt ttttaaaact gaatgggcag gaagatcttt
      181 aacgattgaa acagggcaat tagctaaaca agcaaatggc gctgtattgg ttcgttatgg
      241 agatacagtc gtgttatcga cggcaacggc atcaaaagaa cctcgtgatg gagatttctt
      301 cccattaaca gtgaactatg aagaaaaaat gtacgctgcg ggtaaaattc ctggtggatt
      361 taaaaagaga gaaggacgtc ctggtgacga tgcaacatta actgcgcgat taattgatag
      421 accaattaga cctttattcc ctaaaggata taagcatgat gttcaaatta tgaacatggt
      481 attaagtgca gatcctgatt gttcaccaca aatggctgca atgattggtt catctatggc
      541 gcttagtgtg tcggatattc cattccaagg gccaatcgcc ggtgtaaatg tgggttatat
      601 tgacggtaaa tatatcatta acccaacagt agaagaaaaa gaagtttctc gcttagacct
      661 tgaagtagct ggtcataaag atgcagtaaa catggtagag gcaggcgcta gtgagattac
      721 tgaacaagaa atgttagagg cgattttctt tggtcatgaa gagattcaac gtttagttga
      781 tttccaacaa caaatcgtcg accacattca acctgttaaa caagaattta ttccagcaga
      841 gcgtgatgaa gcgctagttg aacgtgtaaa atctttaact gaagaaaaag gacttaaaga
      901 aacagtttta acatttgata aacaacaacg agatgaaaat cttgataact taaaagaaga
      961 aatcgtcaat gaatttatcg atgaagaaga tccagagaat gaattactta ttaaagaagt
     1021 ttatgcaatt ttaaatgaat tagtgaaaga agaagttaga cgtttaattg cagatgaaaa
     1081 aattagacca gacggccgta aacctgatga aatccgtcca ttagattctg aagttggtat
     1141 tttacctaga acgcatggtt caggtctatt tacacgtggt cagactcaag cactttcagt
     1201 tttaacatta ggtgctttag gcgattatca attaattgat ggtttaggac ctgaagaaga
     1261 aaaaagattt atgcatcatt ataacttccc gaatttttca gtaggtgaaa ctggtccagt
     1321 acgtgcgcca ggtcgtcgtg aaattggaca tggtgcgtta ggtgaaagag cattaaaata
     1381 tattattcca gatactgctg atttcccata tacaattcgt attgtaagtg aggtacttga
     1441 atcaaatggt tcatcatctc aagcgtcaat ttgtggatca acattagctt taatggatgc
     1501 gggcgtaccg attaaagcac cagttgctgg tattgctatg ggtcttgtta cacgtgaaga
     1561 tagctatacg attttaactg atatccaagg tatggaagat gcattaggtg atatggactt
     1621 taaagtcgct ggtactaaag aaggtattac agcaatccaa atggatatta aaattgacgg
     1681 tttaacgcgt gagattatcg aagaggctct agaacaagcg agacgtggtc gtttagaaat
     1741 aatgaatcat atgttacaaa caattgatca accacgtact gaattaagtg cttacgcgcc
     1801 aaaagttgta actatgacaa ttaaaccaga taagattaga gatgttatcg gacctggtgg
     1861 taaaaaaatt aacgaaatta ttgatgaaac aggcgttaaa ttagatattg aacaagatgg
     1921 tactatcttt attggtgccg ttgatcaagc tatgataaat cgtgctcgtg aaatcattga
     1981 ggaaattaca cgtgaagcgg aagtaggtca aacttatcaa gccactgtta aacgtattga
     2041 aaaatacggt gcgtttgtag gcctattccc gggtaaagat gcgttgcttc acatttcaca
     2101 aatttcaaaa aatagaattg aaaaagtgga agatgtttta aaaatcggtg acacaattga
     2161 agttaagatt actgaaattg ataaacaagg tcgagtaaat gcatcacaca gagcattaga
     2221 agaataatat ttaaagtcat atgacgacaa tgtatcgtca tgtgattttt ttatgccatt
     2281 ttttacgaag tgacccattt tgaatttgtt gtattgaaca ttttaaaacg ctttattatt
     2341 ttgtgtgcaa ctgttaatta tcctgtatgt atagtgatta atagtgtaca tcaagtgttt
     2401 tttaacttat aatgaatagt gagtttatat atggacgggt aacaaattta ggaggtaaga
     2461 ttttgagttt aataaagaaa aagaataaag atattcgcat tataccatta ggcggtgttg
     2521 gcgaaattgc taaaaatatg tatatcgttg aagtagacga tgaaatgttt atgttagatg
     2581 ctggacttat gtttccagaa gacgaaatgc taggtattga tattgttata ccagacattt
     2641 catacgtact tgaaaataaa gataaattga agggtatatt ccttacacac ggacatgagc
     2701 acgcgattgg tgcagtgagt tatgttttag aacaattaga tgcaccagta tatggatcta
     2761 aattgacaat agcgttaatt aaagaaaata tgaaagcccg taatattgat aaaaaagttc
     2821 gctactatac agttaataat gattcaatta tgagattcaa aaacgtgaat attagtttct
     2881 ttaatacgac acacagtatt cctgatagtt taggtgtttg tattcacact tcatatggtg
     2941 ccattgtgta tacaggtgaa tttaagtttg accaaagttt acatggacat tatgcaccag
     3001 atattaaacg tatggcagag attggtgaag aaggcgtatt tgtcttaatc agtgattcta
     3061 ctgaggcaga gaaacctgga tataatactc cggaaaatgt aattgaacat catatgtatg
     3121 atgcttttgc aaaagtgcga ggtcgcttga ttgtttcatg ttatgcttcg aactttatac
     3181 gtattcagca agttttaaat attgctagca agctaaatcg taaagtgtca tttttaggaa
     3241 gatcacttga aagttcattt aatattgctc gtaaaatggg gtatttcgac attcctaaag
     3301 atttgctaat tcctataaca gaagttgata attatcctaa aaatgaagtg ataattatag
     3361 ctactggtat gcaaggagaa cctgtagaag ccttaagtca aatggcgcaa cataagcata
     3421 aaattatgaa tatcgaagaa ggcgattctg tatttttagc aattacggct tctgctaata
     3481 tggaagttat cattgcgaat acattaaatg agcttgtacg tgctggcgca catattattc
     3541 caaataacaa gaagattcat gcttcaagtc atggttgcat ggaagaatta aaaatgatga
     3601 ttaatattat gaaacctgaa tactttattc ctgtacaagg tgaatttaaa atgcagatag
     3661 cacatgcgaa gctagcagct gaagcaggtg ttgcaccaga aaagattttc cttgtggaaa
     3721 aaggagatgt cattaattac aacggtaaag atatgatatt aaatgaaaag gtaaattcag
     3781 gaaatatttt aatagatggt attggtattg gggatgtagg aaatatcgtg ttgagagacc
     3841 gtcatctttt agcagaagat ggtatcttta ttgctgtagt aacgttagat cctaaaaata
     3901 gacgtatagc tgcgggacct gaaattcaat ctcgtgggtt tgtatatgta cgtgaaagtg
     3961 aagacttatt acgtgaagca gaagagaaag tacgtgaaat agtagaagct ggtttacaag
     4021 aaaaacgcat agaatggtct gaaattaaac aaaatatgcg tgatcaaatt agtaaactat
     4081 tattcgaaag tacaaaacgt cgtcctatga ttattccagt aatttctgaa atttaatcaa
     4141 aaagtcatta acataaaaga ggtcagaaca agtcaccgaa atataatggt tgtcatggac
     4201 aatttactta tattttatga tggtcaattg aaggggtaac gattaatctg ttatcttaag
     4261 taaattgata catagatgat attgttctaa cctctttcat cgtctgtttg gactacatat
     4321 tctaaacatc aaataggaaa ttatatataa taacgtcgtt ttaactaagg caacataagg
     4381 aggtgcgtca attggcacaa gcaaaaaaga aatcgacagc taagaaaaaa acaacatcaa
     4441 aaaaaagaac aaattcgagg aaaaagaaga atgataatcc gatacgttat gtcatagcta
     4501 ttttagtagt tgtattaatg gtgttgggtg ttttccaatt aggaataata ggtcgtctaa
     4561 ttgacagctt ctttaattat ttatttgggt acagtagata tttaacatat attttagtac
     4621 tcttagcaac tggttttatt acatactcta aacgtattcc taaaactaga cgaacggctg
     4681 gttcgattgt attgcgaatt gcattgctat ttgtatcaca gttagttttt cattttaata
     4741 gtggtatcaa agctgaaaga gaacctgtac tttcttatgt gtatcagtca taccaacaca
     4801 gtcattttcc caattttggt ggcggtgtat taggctttta tttattagag ttaagcgtac
     4861 ctttaatttc attatttggt gtatgtatta ttactatttt attattatgc tcaagtgtta
     4921 ttttattaac aaaccatcaa catcgtgatg ttgcaaaagt tgcactggaa aatataaaag
     4981 cttggtttgg ttcatttaat gaaaaaatgt cggaaagaaa tcaagaaaaa caattgaagc
     5041 gtgaagaaaa agcgagactt aaagaagaac aaaaggcacg tcaaaatgaa cagccacaaa
     5101 taaaagatgt gagtgatttt acggaagtgc ctcaagaaag agatattcca atttatgggc
     5161 atactgaaaa tgaaagtaaa agccagagtc aaccaagtcg aaaaaaacga gtgtttgatg
     5221 cagagaatag ttcgaataac atcgtaaatc atcatcaagc agatcagcaa gaacaattaa
     5281 tagaacaaac tcataacagt gttgaaagtg aaaacactat tgaagaagct ggtgaagtta
     5341 cgaatgtgtc gtatgttgtt ccaccgttaa ctttacttaa tcaacctgca aaacaaaaag
     5401 caacatctaa agctgaagtg caacgtaaag gacaagtact agagaataca ttaaaagatt
     5461 ttggggtaaa tgcaaaagtg acacaaatta aaattggtcc tgcagtaact caatatgaaa
     5521 ttcaaccagc tcaaggggtt aaagtgagta aaattgtaaa cttgcataat gatattgcat
     5581 tagctttagc agcaaaagat gttagaattg aagcgccaat acctggtcgc tctgcagtag
     5641 gtattgaagt gccaaatgag aaaatttcat tagtttcact aaaagaagtt ttagatgaaa
     5701 aattcccgtc taataataaa ctagaagttg gattaggacg agatatatca ggtgatccaa
     5761 ttactgttcc actaaatgaa atgccacact tattggtggc aggatcgacg ggtagtggta
     5821 aatctgtttg tataaatggt attattacaa gtattttatt aaatgctaag ccgcatgaag
     5881 ttaaacttat gttaatcgat ccgaaaatgg ttgaactaaa tgtttataac gggattccac
     5941 atttattaat tccggttgtt acaaatcctc ataaagctgc tcaagcttta gaaaaaattg
     6001 tagctgagat ggaaagacgt tatgatttat tccaacattc atcaactaga aacattaaag
     6061 gttataacga attaatccgt aagcaaaatc aagaattaga tgagaagcaa ccagaattac
     6121 cctatatcgt tgttattgta gatgagcttg cagatttaat gatggtagct ggtaaagaag
     6181 ttgaaaatgc gattcaacgt attacacaaa tggcacgtgc agcaggtata catttgattg
     6241 tagcaacaca aagaccttct gtggatgtaa ttacaggtat cattaaaaat aatattccat
     6301 ctagaattgc ttttgctgtg agttctcaaa cagattcaag aactattatt ggtactggcg
     6361 gcgcagaaaa gttacttggt aaaggtgaca tgttatacgt tggaaatggt gattcatcac
     6421 aaacacgtat tcaaggggcg tttttaagtg accaagaggt gcaagatgtt gtaaattatg
     6481 tagtagaaca acaacaggca aattatgtaa aagaaatgga accagatgca ccagtggata
     6541 aatcggaaat gaaaagtgaa gatgctttat atgatgaagc gtatttgttt gttgttgaac
     6601 aacaaaaggc aagtacatca ttgttacaac gccaatttag aattggttat aatagagcat
     6661 ctaggttgat ggatgattta gaacgcaatc aggtaatcgg tccacaaaaa ggaagcaagc
     6721 ctagacaagt tttaatagat cttaataatg acgaggtgta aaaaaatgtc agaaatgaat
     6781 gcggtatata acgttaaaca atacatttta aatttgatta agcaaaataa attggaatat
     6841 ggtgaccaac ttccaagtaa tttatcaatt gccagagaat taaatgtaaa aaccgacgat
     6901 gtttatgaag caattcaggc attgattact gaacaagtca ttaaagataa ttttgaagag
     6961 ggcacaagtg ttaagtcact gccccctttc ttttatccat tgaatgaact tataagtatt
     7021 gggcaaatga ttaaaaatgc aggatttgaa tgcggaactg aatacttaaa ttttgatgag
     7081 caaccagcaa ctatgttaga tgcaaatttg ttgagcgttg aagaaggata ttcagtaacc
     7141 attatagaac gattacgaac tgccgatgga gaaccggtcg tctattgttt agataaaatt
     7201 gctaaaaaag aattaacatg tacagagtat caaatgagca atggatcgat actaagtgcg
     7261 ataaaagaac aaagtaatca taatatttgt tacgctgata cagaaattga agcggtaaat
     7321 tatgaacctc gaatatccga agtacttaat gcttcgccac acgaaggttt gattttatta
     7381 aaaattacgc actacaatga attagatgaa cctattttgt attcattaaa ttatatgaaa
     7441 aatagcttag ttcaatttaa aatcactaga aaaatataga tgtaagaaga tatagaggag
     7501 gctatatatt tgagtagaca atctcaacca aatatacata tcaaagtttc accaacaact
     7561 aaatttaaaa caactactat agtttttaaa tttatggcac ctttagaata tgacacaata
     7621 acaggtagat cattattaag caaattatta gttcgagcaa ctaagaaatg gccaaccgat
     7681 aagtcattta ataatcactt agccgattta tatggtgcgt atgtgaatag tacaatttca
     7741 aaattcaaag atcagcatgt cattacattt tcattagaaa ttgttaatga acgttattta
     7801 agaaacggtg aatcattatt taatcaagga ttagatttat tacaagaaat catttggaat
     7861 ccattaattg aaaataaagc attcaatgat aattttgtta accaagagaa aacattatta
     7921 gccaaaaaaa tagaagctat ggtagataac aaagcgcaat attcgttttt aaaattactc
     7981 gaccatatgt ttgaaaatga agcatacaaa tacttatcta caggacaact agaacaaatc
     8041 ccacatatta ctgctgaaac actatatcat acatatcaat caatgattaa taatgatcaa
     8101 tgttctgttt atgttgtcgg caatgtagaa cctgaaagtg ttgagaaaca aatacgtgaa
     8161 aaatttgcac ttaaaccatt cgataaacat caattccaac attctactca tcatttacac
     8221 gatgaagaag ttgattatat tgttgaatat gatgacgtgg atcaagctaa attaaatatg
     8281 ggataccgtt ttccaacaca atatggtcaa agtggatatg ctgcctttgt tgtatttaac
     8341 atgatgtttg gaggagatcc ttcatctgtt ttatttaatg aagtgcgaga aaagcaaagt
     8401 ttagcgtact ctatacattc acaaattgat ggcaaaaatg gctatttatt tgttttgagt
     8461 ggggtttcaa gcgataagta cgaaactgca aaaaacacta ttataagtga atttgaaaaa
     8521 ataaaagcag gagatttcac tgaagaaaaa ttagagttag ctaaaaaagt aatcatttct
     8581 catcgatatg aatctgaaga tcgtccgaaa agtgttatag agattatgca taaccaaata
     8641 ttattagagc aaccacaaag caaagaaaca tttataaaag atatacagaa ggtaagtcgc
     8701 gaagatattg tttctgttgc tgaaaaagca tttttagata caatctatgt gttgacaaaa
     8761 ggaggggata aataatgaaa gagcgttatt atgaattaat agacgaaaga gtattcgaac
     8821 aagaattaga aaatggttta cgattattta ttatccccaa accaggtttt caaaagacat
     8881 ttgtcactta cactacacaa tttggttcat tagataatca attcaaaccc attggacaag
     8941 accaatttgt tactgtacct gatggagttg ctcacttttt agaacataaa ttatttgaaa
     9001 aagaagaaga agacttattt actgcgtttg ctgaagataa tgcacaagca aatgcgttta
     9061 caagctttga tcgtacaagc tacttgttca gtgcaactga taatattgaa aacaacatta
     9121 aacgtttact tacaatggtt gaaacgcctt attttacaaa agaaactgtt gataaagaaa
     9181 aaggtattat tgcagaagaa ataaaaatgt atcaagaaca acctagatat aaattaatgt
     9241 tcaatacatt gcgtgcaatg tatcaacaac atccaatacg tgttgatatt gccggtagtg
     9301 tagaaagcat atacgatatt acaaaagatg atttgtatct atgttatgaa acgttttatc
     9361 atccatcaaa tatggtttta tttgttgttg gtgatgtgga tcctgaaaca atatgtcgaa
     9421 tagtaaaaca acacgaggat gctcgtaata aagttaacca acccaaaatc gatcgaggac
     9481 ttgttgatga accggaggat gttaaagaag catttgttac tgaatctatg aaaattcaat
     9541 caccaagact aatgcttggt tttaaaaata aacctttaca agaagcgcct caaaaatatg
     9601 tacaacgtga tttagaaatg tcattattct ttgagttgat ttttggggaa gaaacagatt
     9661 tttatcagaa tttattaaac gaaggactta tcgatgatac atttggttat caatttgtac
     9721 tagagccgac gtatagtttt tcaatcgtga caagcgctac tgaagaacca gataaattaa
     9781 aaaaattatt attagatgag ttgtgtgata aaaaaggcaa tttccaagat gcagaagcat
     9841 ttgaactttt gaaaaagcaa tttataggtg aattcatatc aagtttaaac tcacctgaat
     9901 atattgctaa tcaatatact aaattgtatt ttgaaggtgt tagtgtgttt gacatgttag
     9961 acattgttga aaacatcacg ttagatagta ttaatgaaac gtcatcgtta tatttaaatt
    10021 tagatcagca agtcgatagt cgtttggaga ttaaaaagta atgaaagcat tagtattagg
    10081 tggttctggt tcaattggtt ctgagatagt caaacaatta ttaactgatg gatttgaagt
    10141 ttatgtgcaa tattatcgta ctgatataaa tgaattaact agcaaattta atgatgacaa
    10201 agtgcatttt atacaagcgg atttatctca aacaattgat attgacaaaa catttggtga
    10261 cattaaatca ttagactgtt taatatatgc aagtggtcag tctttatatg gtgttttaca
    10321 agatatgaaa gaccatgata ttgatgcatg ttatcagtta aatgtcttgc aattaattcg
    10381 attatgtaga tatttcgttg atgttttacg tcaaagtgac aatggaagaa ttattgtaat
    10441 ttcatcaatt tggggtgaga caggagctag tatggaaact atttattcga cgatgaaaag
    10501 tgcacaatta ggtttcgtta aggcgcttag tcaagagctt gcactaacat cagtgacagt
    10561 aaatgctatc gcacctggat ttgtagccgg taatatggca agtgagtggc aagaagatga
    10621 acttcaagca atgataactg aattaccaca acagcgattg gttttaccga gtgaggttgc
    10681 tcatacatgc gcctatttat atcacccaaa tgctagaagt gttactggaa ctatacagaa
    10741 agttaatggt gcttggtata tttaaaatat agaactattt ttatgagcaa atgtttttat
    10801 gattgatgtt ataatatgaa tattacaatg aatcataatt tgttaattaa acaaggggtg
    10861 aggttcacca tgacagttgc agagaaaaaa gaatggtact tagaatacga aattgaaatt
    10921 aatagaccgg gtcttttagg tgatgtatct agtttattag gtatgttagg tataagtatt
    10981 gttacaatta atggtgttga tcaaggtaaa cgaggccttt taattaaaac agacaatctt
    11041 gaaaaagttg aacgttttga gcaaatagct cgtggtataa atgaaattga aataacaaag
    11101 cttaaaaaac cagaattaag agaccgtctt gcagttagac atggtagata tattgagcaa
    11161 gatgcaaaag ataagaaaac ctttcgattt gagcgtgaag atttaggctt gttagtagac
    11221 tttttagctg aattgttcaa agaagaaggt cataagttga ttggcattag aggtatgcca
    11281 cgggttggta aaactgaatc aattgttgcg ggaagtgttt gtgcacataa gagatggtta
    11341 tttattagtt ctactttaat aaaacaaact gtacgtagct ctcttattaa aggggaatat
    11401 gatgccaatc atgtatacat tattgatggt gcagttactg ccagagaatc taatccaaaa
    11461 catcaagagc ttgttaacga agttatgacg ttaccttcaa tcaaagtcgt tgaacaccca
    11521 gatttatttg ttgaaacaag tacttgtaca atggaagatt ttgactatat tatcgaattg
    11581 agagaaaatg aaaatcaaga aatacattac gaagaaatga agaaacagac agtccaaagt
    11641 aagaacaatt tagattttgg agatccattt ggtggtggtt ttggtttctt cgagtaagtg
    11701 taaggaggct atgaattgaa aacggtcggt gaagcgctaa aaggtagacg tgaaaggtta
    11761 ggaatgactt taacagaatt agagcaacgt actggaatta aacgtgaaat gctagtgcat
    11821 attgaaaata atgaattcga tcaactaccg aataaaaatt acagcgaagg atttattaga
    11881 aaatatgcaa gcgtagtaaa tattgaacct aaccagttaa ttcaagctca tcaagatgaa
    11941 attccatcga accaagccga atgggacgaa gtaattacag ttttcaataa taataaagac
    12001 ttagattata agagtaaatc aaaagagcca atacaattat tagtaatcat gggtattaca
    12061 gttttaataa ctttattgtt atggatcatg ttagttttaa tattttaaca gaaataaatt
    12121 agtgagaaat gaggatgtat aatgaatatt ccgaaccaga ttacggtttt tagagtagtg
    12181 ttaataccag tttttatatt gtttgcgtta gttgattttg gatttggcaa tgtgtcattt
    12241 ctaggaggat atgaaataag aattgagtta ttaatcagtg gttttatttt tatattggct
    12301 tcccttagcg attttgttga tggttattta gctagaaaat ggaatttagt tacaaatatg
    12361 gggaaatttt tggatccatt agcggataaa ttattagttg caagtgcttt aattgtactt
    12421 gtgcaactag gactaacaaa ttctgtagta gcaatcatta ttattgccag agaatttgcc
    12481 gtaactggtt tacgtttact acaaattgaa caaggattcg taagtgcagc tggtcaatta
    12541 ggtaaaatta aaacagcagt tactatggta gcaattactt ggttgttatt aggtgatcca
    12601 ttggcaacat tgattggttt gtcattagga caaattttat tatacattgg cgttattttt
    12661 actatcttat ctggtattga atacttttat aaaggtagag atgtttttaa acaaaaataa
    12721 atatttgttt atactagatt tcattttcat atggaatcta gtttttttaa tcccaatttt
    12781 agaaattagc cacgcatttg tttataatga tatattgtaa aacaatattt gttcattttt
    12841 tagggaaatg tgttgtagca tctgatacat tgaatttaaa attgatgtga atttttaaat
    12901 ggaatacatg aaaaaatgaa ttaaacgata caagggggat ataaatgtca attgccatta
    12961 ttgctgtagg ctcagaacta ttgctaggtc aaatcgctaa taccaatgga caatttctat
    13021 ctaaagtatt taatgaaatt ggacaaaatg tattagaaca taaagttatt ggagataata
    13081 aaaaacgttt agaatcaagt gtacgtcatg cgttagaaaa atatgatact gttattttaa
    13141 caggtggctt aggtcctacg aaagatgact taacgaagca tacagtggcc cagattgttg
    13201 gtaaagattt agttattgat gagccttctt taaaatatat tgaaagctat tttgaggaac
    13261 aaggacaaga aatgacacct aataataaac aacaggcttt agtaattgaa ggttcaactg
    13321 tattagcaaa tcatcatggc atggctccag gaatgatggt gaattttgaa aacaaacaaa
    13381 ttattttatt accaggtcca ccgaaagaaa tgcaaccaat ggtaaaaaat gaattgttgt
    13441 cacattttat aaaccataat cgaattatac actctgaact attaagattt gctggaatag
    13501 gtgaatctaa agtagaaaca gtattaatag atcttatcga taaacagact aatcctacga
    13561 ttgcgccttt ggcaggaagt catgaagtat atattagatt gactgcaaat gccgactcaa
    13621 aagaacaagc acaatcattg attcaacctg ttaagcaaga aattcttgat cgtattggag
    13681 aatattatta tggttcagat gacacattaa ttgagcaagc tgtaataaag aaaattaatg
    13741 aaccttttgt aatatatgat ggtattacta atggtgcttt atatcatcga ttgaaagaag
    13801 tggatttaaa cgatgttcta aagggtatga ttaatcacaa tgaaaacttt gttgatatta
    13861 ataaacctat tgagcagcaa ttaaaagatg cagtgcaatt tgttaataaa ttatttaatg
    13921 tgtcatcagc aattattcta ttagagtatg atggtgtagt ccatataggc tatgataata
    13981 acttagaatt taaaactgag caatttaaaa tgtctaaatc tagaaattta ttaaagaaca
    14041 gaagtcaaaa ttatgcgctt ataaggttat taaattggct tagaacaaca aattaattgt
    14101 attatcgata aagatataag cacgtttgtt cgtttttgtg ttttgatttc aagattttac
    14161 acgaacaaat attcgcaaaa cacttgtatt ttattttgaa tccttgtata gtattggtaa
    14221 gataattaaa agatagcaat ttcaattagg aggtctcgct ttggataacg atcgtcaaaa
    14281 agctttagat acagtaatta aaaatatgga gaaatctttc ggtaaaggtg ccgtaatgaa
    14341 gttgggtgac aatataggtc gccgagtttc aactacatca actggttcag ttacattaga
    14401 taatgcgcta ggtgtaggtg gctatcctaa aggacgaatt attgaaattt atggtcctga
    14461 aagttctggt aagacaacag tagcgcttca cgctattgct gaagttcaaa gtaatggcgg
    14521 ggtggcagca tttatcgatg ctgaacatgc tttagaccca gaatatgctc aagcattagg
    14581 cgtagatatc gataatttat atttatcgca accggatcat ggtgaacaag gtcttgaaat
    14641 cgccgaagca tttgttagaa gtggtgcagt tgatattgta gttgtagact cagttgctgc
    14701 tttaacacct aaagctgaaa ttgaaggaga aatgggagac actcacgttg gtttacaagc
    14761 tcgtttaatg tcacaagcgt tacgtaaact ttcaggtgct atttctaaat caaatacaac
    14821 tgctattttc atcaaccaaa ttcgtgaaaa agttggtgtt atgttcggta atccagagac
    14881 tacaccaggt ggacgtgctt taaaattcta tagttcagta agactagaag tacgtcgtgc
    14941 agaacagctt aaacaaggac aagaaattgt aggtaataga actaaaatta aagtcgttaa
    15001 aaataaagtg gcaccaccat ttagagtagc tgaagttgat attatgtatg gacaaggtat
    15061 ttctaaagag ggtgaactta ttgatttagg tgttgaaaac gacatcgttg ataaatcagg
    15121 agcatggtat tcttacaatg gcgaacgaat gggtcaaggt aaggaaaatg ttaaaatgta
    15181 cttgaaagaa aacccacaaa ttaaagaaga aattgatcgt aaattgagag aaaaattagg
    15241 catatctgat ggtgatgttg aagaaacaga agatgcacca aagtcattat ttgacgaaga
    15301 atagtacaca aatttatatc tatagttaaa cttagcaaat atccttatag gattgattga
    15361 aagtgatatt catctcataa agctggaata atatctaact ttatgggatg cacttcaaat
    15421 cgagactata aggtttttta tgttatttac tattacatta tcaatagttt tataatcgag
    15481 cttcaaaact ttagaaaata gtagaaatag cattcaataa agtgcaaaag tgcaaattga
    15541 taacttgaca cttatctcct ataaaccgta caattaattt gtatgattta tatataattt
    15601 cataaagtca tattgaattt catataaaga gcaaacccta gaaaaggagg tgtttgtgtg
    15661 aatttattaa gcctcctact cattttgctg gggatcattc taggagttgt tggagggtat
    15721 gttgttgccc gaaatttgtt gcttcaaaag caatcacaag ctagacaaac tgccgaagat
    15781 attgtaaatc aagcacataa agaagccgac aatatcaaaa aagagaaatt acttgaggca
    15841 aaagaagaaa accaaatcct aagagaacaa actgaagcag aactacgaga aagacgtagc
    15901 gaacttcaaa gacaagaaac ccgacttctt caaaaagaag aaaacttaga gcgtaaatct
    15961 gatctattag ataaaaaaga tgagatttta gagcaaaaag aatcaaaaat tgaagaaaaa
    16021 caacaacaag tagatgcaaa agagagtagt gttcaaacgt taataatgaa gcatgaacaa
    16081 gaattagaac gcatctccgg tctcactcaa gaagaagcta ttaatgagca acttcaaaga
    16141 gtagaggaag aactgtcaca agatattgca gtacttgtta aagaaaaaga aaaagaagct
    16201 aaagaaaaag ttgataaaac agcaaaagaa ttattagcta cagcagtaca aagattagca
    16261 gcagatcaca caagtgaatc aacggtatca gtagttaact tacctaatga tgagatgaaa
    16321 ggtcgaatca ttggacgaga aggacgaaac atccgtacac ttgaaacttt aactggcatt
    16381 gatttaatta ttgatgacac accagaagca gttatattat ctggttttga tccaataaga
    16441 agagaaattg ctagaacagc acttgttaac ttagtatctg atggacgtat tcatccaggt
    16501 agaattgaag atatggtcga aaaagctaga aaagaagtag acgatattat aagagaagca
    16561 ggtgaacaag ctacatttga agtgaacgca cataatatgc atcctgactt agtaaaaatt
    16621 gtagggcgtt taaactatcg tacaagttac ggtcaaaatg tacttaaaca ttcaattgaa
    16681 gttgctcatc ttgctagtat gttagctgct gagctaggcg aagatgagac attagcgaaa
    16741 cgagctggac ttttacatga tgttggtaaa gcaattgatc atgaagtaga aggtagtcat
    16801 gttgaaatcg gtgtagaatt agcgaaaaaa tatggtgaaa atgaaacagt tattaatgca
    16861 atccattctc accatggtga tgttgaacct acatctatta tatctatcct tgttgctgct
    16921 gcagatgcat tgtctgcggc tcgtccaggt gcaagaaaag aaacattaga gaattatatt
    16981 cgtcgattag aacgtttaga aacgttatca gaaagttatg atggtgtaga aaaagcattt
    17041 gcgattcagg caggtagaga aatccgagtg attgtatctc ctgaagaaat tgatgattta
    17101 aaatcttatc gattggctag agatattaaa aatcagattg aagatgaatt acaatatcct
    17161 ggtcatatca aggtgacagt tgttcgagag actagagcag tagaatatgc gaaataattt
    17221 ttgtctccct cacaaattag tgagggagct tttttaagtt gtagtctcaa tctagttaga
    17281 cagcacttta ttggtaataa ctatattaaa cagtagttat ttgaaagtaa gacgtacctt
    17341 atattaaata agaagttatt gcttttaata aaaatgtttt aggttcataa ttactatatt
    17401 tatattatgt aaacctataa aggtgattgc tttttctaac taacaaaaaa gaagagaaga
    17461 tgtaacacat cttctcttct gcaatattaa ttagtattta tttctaaggt gagttatttt
    17521 aattgtaaat ctgttttctt taattctttt ataacttctg cagtatcata acaatttgtt
    17581 gcaattgttg aatatctctc tgctaaacga tatgcattaa tgtaaagctt taaactttct
    17641 ttagctatat cctctgcatc ttcgaatttt gatgggttag acataactac taattctgca
    17701 aatttttctg gatcaatatt aatagacatg tatttattta caactcctat ttattttgat
    17761 gtcttaatac taacatattg aagttttcag acaaagtaat gtctctctat aattgaagaa
    17821 aaataattca aagaatttaa tatttacttt aaatattaga acgtatgaat taaactagta
    17881 tttatgagag gatgaacaaa acatgagaat aatgtttata ggggatatcg taggtaaaat
    17941 tggacgagac gcaattgaaa cgtacatacc tcaactgaag caaaagtata aaccaacagt
    18001 tacaattgta aatgctgaaa atgcagcaca tggtaaaggt ttgactgaaa aaatatataa
    18061 acaattacta agaaatggtg tagatttcat gactatgggt aatcacacat atggtcaacg
    18121 tgaaatttat gattttatag atgaagctaa acgactagta agaccagcga attttccgga
    18181 tgaagcgccg ggaattggta tgagatttat acaaattaat gatattaaac ttgcagttat
    18241 taatctgcaa ggaagagcgt ttatgccaga tattgatgat ccttttaaaa aggcagatca
    18301 attagtcaag gaagcacaag aacaaactcc gtttatattt gttgattttc atgcagaaac
    18361 aacttctgaa aagtatgcaa tgggatggca tttagatggt agagctagcg ctgttgttgg
    18421 aacgcataca cacattcaaa cagcagatga acgtatttta ccaaagggta cagggtatat
    18481 aacggatgtt ggtatgacag gcttttatga cggcatttta ggaataaata aaacagaggt
    18541 aattgagcgt tttatcacta gtttgccaca aagacatgtt gttccaaatg aaggtagaag
    18601 tgtattatct ggtgttgtta ttgatttgga caaagaaggt aaaacaaagc acatcgaacg
    18661 tatattgata aatgatgacc atccattttc aacattttaa aattacgtaa gtaaacattc
    18721 taattggacc ctatcgtcca ttagtatgaa tttaatatag taccactgtt tacatagtaa
    18781 atcggtggtt ctttttgtta tcatttaata tgaaatatat ccataggagg catataacta
    18841 tgaaaccaca attatcgtgg aaagttggcg gtcaacaagg cgaaggtatt gaatcaactg
    18901 gggaaatctt cgctacggct atgaatagaa aaggatatta tttatatgga tatagacatt
    18961 tttcaagtcg tatcaaaggt ggacatacga ataataaaat tagagtttct acgacgcctg
    19021 ttcatgcaat tagtgatgat ttagatattt tgattgcatt tgaccaagaa acaattgatg
    19081 ttaaccatca tgaaatgaga gaagacagta tcattttagc tgatgccaag gctaaacctg
    19141 tgaaaccaga aggatgtcat gcacagctta ttgaattacc ttttacagca accgctaaag
    19201 aattaggtac agcattaatg aaaaacatgg ttgcaatagg tgctactagc gcattgatga
    19261 atttgaatac aaatacattt gaagaactta ttactaatat gttttctaaa aaaggtgaca
    19321 aggtagttga agtcaatatc caagcattaa acgaaggtta tcaattaatg caatctcgct
    19381 tacctgaaat cgacggggac tttgaattag agtcaacaga tgcactacca catctatata
    19441 tgattggtaa cgatgccatt ggattaggtg caattgctgc aggttcacaa tttatggcgg
    19501 catatcctat tacacctgcg tctgaagtta tggaatatat gattgccaat atatctaaag
    19561 taaacggagc ggttattcaa acagaagatg aaattgctgc tgtaactatg gctattggtg
    19621 caaattatgg tggagtcaga gcgtttacgg ctagtgctgg tccaggttta tctttgatga
    19681 tggaagcaat tggattatct ggtatgactg aaacgccatt agtcattatt aatacccaac
    19741 gaggtggacc ttctactgga ttacctacga aacaagaaca gtcagattta atgcaaatga
    19801 tttatggtac acatggtgat attccaaaaa ttgttgtagc accaactgat gcagaagatg
    19861 cattttattt aactatggaa gcctttaatt tagcagaaca atatcaatgc cctgttatag
    19921 ttctaagtga tttgcaatta tctttaggaa aacaaactgt tgaaaaatta gattataatc
    19981 gcattgaaat taaacgtggt gaaatcattc aatctgatat tgaacgtgaa gaagatgata
    20041 aaggttattt caagcgttat gcgttaacat ccaatggtgt ttctcctaga cctatcccgg
    20101 gtgttaaagg aggtattcat catataactg gtgtggaaca caatgaagaa ggtaaaccta
    20161 gtgaatctgc gtcaaataga caacaacaaa tggaaaaacg aatgcgtaaa attgagcagt
    20221 tactaattga atcgccagta gaagctaact tacaacatga ggatgctgat attctttata
    20281 tcggttttat ttctacaaaa ggtgcaattc aagaaggtag taaccgtttg aatcaacaag
    20341 gcataaaagt taacactata caaattagac aattgcatcc attcccaaca agcgttattc
    20401 aagatgcagt taataaagcg aagaaagtcg ttgtagtgga gcacaattat caaggacaat
    20461 tggctagtat tataaaaatg aatgtcaata ttcatgataa gattgaaaat tatacaaagt
    20521 atgatgggac acctttccta ccacatgaaa tcgaagaaaa aggtaaaata attgctactg
    20581 aaataaagga gatggtatag atggcgacat ttaaagattt tagaaataat gttaagccta
    20641 actggtgccc tggatgtggc gatttctcag tacaagctgc aattcaaaaa gcagccgcaa
    20701 atatagggtt agaacctgaa gaagtagcta tcatcaccgg tataggatgt tctggccgtc
    20761 tttcaggata tattaattct tatggcgttc actctattca cggacgtgca ttacctttag
    20821 ctcaaggtgt aaaaatggcg aataaagatt taactgttat tgcatctgga ggagatggtg
    20881 atggttatgc tataggtatg gggcatacaa tccatgcttt aagaagaaat atgaacatga
    20941 cgtatatagt catggataat caaatttatg gtttgacaaa gggacaaaca tcgccgtcat
    21001 cagcagtagg attcgttact aaaacaacgc caaaaggtaa tatagaaaaa aatgttgcgc
    21061 ctttagaatt agcattatca tctggtgcca catttgtagc ccaaggtttt tcaagtgata
    21121 ttaaaggatt aacaaaacta attgaagatg caattaatca cgatgggttt tcatttgtta
    21181 atgtcttttc accatgtgtg acttataata aaattaacac atacgattgg tttaaagaac
    21241 atttaacaag tgttgatgac attgaaaact atgattcaac agataaacaa ttagcgacta
    21301 aaactgttat tgaacatgaa tctttagtaa ctggtattgt ttatcaagat aaagaaacac
    21361 catcatatga atctcaaatt aaagagttag atgatacacc actagctaaa agagatatca
    21421 aaattactga agacacgttc aatgcattaa ctgaacaatt tatttaataa ttaaaactga
    21481 gtcgtataaa tgtatttata acagatccat ttatgctact cagttttttc
//
LOCUS       DAPIDM010000030        21391 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000030 DAPIDM010000000
VERSION     DAPIDM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21391
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.030"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..469
                     /locus_tag="RJE42_001029"
     rRNA            <1..469
                     /locus_tag="RJE42_001029"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            543..657
                     /gene="rrf"
                     /locus_tag="RJE42_001030"
     rRNA            543..657
                     /gene="rrf"
                     /locus_tag="RJE42_001030"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            669..743
                     /locus_tag="RJE42_001031"
     tRNA            669..743
                     /locus_tag="RJE42_001031"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:701..703,aa:Asn,seq:gtt)
     gene            745..816
                     /locus_tag="RJE42_001032"
     tRNA            745..816
                     /locus_tag="RJE42_001032"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Glu,seq:ttc)
     gene            824..899
                     /locus_tag="RJE42_001033"
     tRNA            824..899
                     /locus_tag="RJE42_001033"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:857..859,aa:Val,seq:tac)
     gene            928..1011
                     /locus_tag="RJE42_001034"
     tRNA            928..1011
                     /locus_tag="RJE42_001034"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:962..964,aa:Tyr,seq:gta)
     gene            1021..1095
                     /locus_tag="RJE42_001035"
     tRNA            1021..1095
                     /locus_tag="RJE42_001035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1099..1171
                     /locus_tag="RJE42_001036"
     tRNA            1099..1171
                     /locus_tag="RJE42_001036"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1132..1134,aa:Lys,seq:ttt)
     gene            1313..2221
                     /gene="rocF"
                     /locus_tag="RJE42_001037"
     CDS             1313..2221
                     /gene="rocF"
                     /locus_tag="RJE42_001037"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDV5876469.1"
                     /translation="MTKKKGIDIIGVPSTFGQRKLGVDLGPTAIRYAGLIPRLKQLDL
                     DVNDKGDIKVSAVDIEKFQSEQNGLRNYDEIIDVTQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLTYRESHFALELLHQSQSVTSMDLVEVN
                     PLIDHNNHTAEQAVSLIGTFFGETLL"
     gene            2410..3219
                     /locus_tag="RJE42_001038"
     CDS             2410..3219
                     /locus_tag="RJE42_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDV5876470.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            3221..4153
                     /locus_tag="RJE42_001039"
     CDS             3221..4153
                     /locus_tag="RJE42_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDV5876471.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            4180..5535
                     /locus_tag="RJE42_001040"
     CDS             4180..5535
                     /locus_tag="RJE42_001040"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV5876472.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            5818..13179
                     /locus_tag="RJE42_001041"
     CDS             5818..13179
                     /locus_tag="RJE42_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HDV5876473.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISINPTTASAAEQNQP
                     AQNQPAQPADASAQPNANAGAQANPTAQPAAPANQGQPAAQPANQGGQANPAGGAAQP
                     AAGTTQPAGQGNQADPNNAAQAQPGNQAGQGNNQATPNNNATPANQTQPANAPAAAQP
                     AAPVAANAQTQDPNASNTGEGSINTTLTFDDPAISTNDNRQDPTVTVTDHVNGYSLIN
                     NGKIGFVNSELRRSDMFDKNNPQNYQARGNVAALGRVNANDSTDHGNFNGISKTVNVK
                     PDSELIINFTTMQTNSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDADRLD
                     LQFIPDNTAVADASRITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNKEYT
                     INTEIGNNGNFGASLKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLKNMTVNY
                     DQNANKVTFTSQGVTTARGTHTKEVLFPDKSLKLSYKVNVANIDTPKNIDFNEKLTYR
                     TASDVVINNAQPEVTLTADPFSVAVEMNKDALQQQVNSQVDDSHYTTASIAEYNKLKQ
                     QADTILNEDANHVETANRASQADIDGLVTKLQAALIDNQAAIAELDAKAQEKVTAAQQ
                     SKKVTQDEVAALVTKINNDKNNAIAEINKQTTAQGVTTEKDNGIAVLEQDVITPTVKP
                     QAKQDIIQAVTTRKQQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQVEAI
                     KTKAINDINQTAPATTAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERINAAK
                     VSGVKAIEATTTAQDLERVKNEEIFKIENITDSTQTKMDAYKEVKQAATARKAQNATV
                     SNATDEEVAEADAAVDAAQTEGLHDIQVVKSQQEVADTKAKVLDKINAIQTQARVKPA
                     ADTEVENAYNTRKQEIQNSNASTTEEKQAAYTELDTKKQEARTNLDTANSNSEVATAK
                     DNSIAAINQVQAATTKKSDAKAEIAQKASERKTAIEAMNDSTTEEQQAAKDKVDQAVV
                     NANADIDNAAANNDVDNAKTTNEATIAAITPDANVKPQAKQAIADKVQAQETAIDANN
                     GATTEEKTAAKQQVQTEKTTADAAIDGAHSNAEVEAAKNAEIAKIEAIQPATTTKDDA
                     KQAIATKANERKAAIAQTQEITAEEIAAANANVDNAVTEANNHIETANSQNEVDQAKT
                     TGEASIDQVTPTVNKKATARNEITTILNNKLQAIQATPDATDEEKQAAETEANTENAK
                     ANQAITAATTNAEVDEAKTNAEAAINAVTPKVMKKQAAKDEIDQLQAAQTAVINNDQN
                     ATNEEKEAAIQQLATAVTDAKNNITAATDNNGVDTAKDAGKNSIQSTQPATAVKSNAK
                     NEVDQAVTTQNQAIDNTTGATTEEKNAAKDLVSKAKEKAYQDILNAQTTNDVTQIKDQ
                     AVADIQGITADTTIKDVAKDELATKAKEQKALIAQTADATTEEKEQANQQVDAQLTQG
                     NQNIENAQSIDDVNTAKDNAIQAIDPIQASTDVKTNARAELLNEMQNKITEILNDNTT
                     TNEEKGKDIEPVRAAYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPAGKT
                     ELDQAAADKKTQIEQTPNASQQEINDAKQEVDAALNQAKTNIDQSSTNEYVDNAVKEG
                     KAKINAVKTFSEYKKDALAKIADAYNAKVNEADNSNASTSSEIAEAKQKLAELKQTAD
                     QNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNILADTNAT
                     QDEKQQAIKQVDQNVQTALESINNGVDNSDVDDALTQGKAAIDAIQVDATVKPKANQA
                     IDDKAQETKESIDQSDQLTAEEKTAALATIKQITDQAKQGITDATTTAEVEKAKAQGL
                     EAFDNIQIDSTEKQKAIEELETSLDQIEAGVNVNVDATTEEKEAFTNALEDILSKATE
                     DISDQTTNAEIATVKNSALEQLKAQRINPVVKKNALEAIREVVNKQIEIIKNADADAS
                     AKEIARTDLGRYFDRFADKLDKTQTNAEVDELQNVTIPAIEAIVPQKDPNANDTNSGS
                     DNNDATANSNANATPENTGQPNVTESTDNANADTSSTTTNNQNDAATGGTTATSTNNS
                     SVDASTDNSATGNGTTDKPATETDNATPAEGTRNNNGTATATNENAPTGSTATAPTTN
                     ASTGAASSADSKDNASVNDSKQNAEVNNSAESQSTNGKVAQPKSENKAKAEKDGRDST
                     NQSMVESTTETLPSADITGPKVPSNTEKDKEESTTNQTDAGQLKSETNVASNEADKSE
                     GNVDTDVSNKPSTSASSEAKEKMTSINVSQKDDTATADTNDTQTSVGPVANNKATQND
                     GTNASPATVSNGSNSANQDMLNVTNTEENKANAKSAQQGKVNKLKQQAKTLPDTGMSH
                     NDDLPYAELALGAGMAFLIRRFTKKDQQTEE"
     gene            complement(13498..14604)
                     /locus_tag="RJE42_001042"
     CDS             complement(13498..14604)
                     /locus_tag="RJE42_001042"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV5876474.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIETYNSLS"
     gene            complement(14604..15038)
                     /locus_tag="RJE42_001043"
     CDS             complement(14604..15038)
                     /locus_tag="RJE42_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV5876475.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(15050..17182)
                     /locus_tag="RJE42_001044"
     CDS             complement(15050..17182)
                     /locus_tag="RJE42_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDV5876476.1"
                     /translation="MLLSIREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QITKSSYLTLTVHIVLSISRIKNGEYVALNDDIYESVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVKKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQKTHDKSSNMI
                     DKDDVHVETKDIDDNTSFENEQTSNLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPSSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLISDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(17217..18755)
                     /locus_tag="RJE42_001045"
     CDS             complement(17217..18755)
                     /locus_tag="RJE42_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV5876477.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWFPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFFGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTKEPKQDLEAATAQMETTKGKKSSVASKLVSSDKNVNKEENASGNVSETS
                     SLDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            19436..21241
                     /gene="glmS"
                     /locus_tag="RJE42_001046"
     CDS             19436..21241
                     /gene="glmS"
                     /locus_tag="RJE42_001046"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV5876478.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKGADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDRDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVTLQLISYYAALHRDLDVDKPRNLAKSVTVE"
BASE COUNT         7880 a         3559 c         3932 g         6020 t
ORIGIN      
        1 ggataaaagc taccccgggg ataacaggct tatctccccc aagagttcac atcgacgggg
       61 aggtttggca cctcgatgtc ggctcatcgc atcctggggc tgtagtcggt cccaagggtt
      121 gggctgttcg cccattaaag cggtacgcga gctgggttca gaacgtcgtg agacagttcg
      181 gtccctatcc gtcgtgggcg taggaaattt gagaggagct gtccttagta cgagaggacc
      241 gggatggaca tacctctggt gtaccagttg tcgtgccaac ggcatagctg ggtagctatg
      301 tgtggacggg ataagtgctg aaagcatcta agcatgaagc ccccctcaag atgagatttc
      361 ccaacttcgg ttataagatc cctcaaagat gatgaggtta ataggttcga ggtggaagca
      421 tggtgacatg tggagctgac gaatactaat cgatcgaaga cttaatcaaa tttaaatgtt
      481 ttgcgaagca aaatcaattt tacttactat ctagttttga atgtataatc tacattcata
      541 tgtctggtga ctatagcaag gaggtcacac ctgttcccat gccgaacaca gaagttaagc
      601 tccttagcgt cgatggtagt caaacttacg ttccgctaga gtagaacgtt gccaggcatt
      661 aatattaatc cacagtagct cagtggtaga gctatcggct gttaaccgat cggtcgtagg
      721 ttcgagtcct acctgtggag ccatggctcc ttggtcaagc ggttaagaca ccgccctttc
      781 acggcggtaa cacgggttcg agtcccgtag gagtcattat attggagaat tagctcagct
      841 gggagagcat ctgccttaca agcagagggt cggcggttcg aacccgtcat tctccaccat
      901 tttgattatt aattaactga ataagctgga ggggtagcga agtggctaaa cgcggcggac
      961 tgtaaatccg ctccttcggg ttcggcagtt cgaatctgcc cccctccacc atctatatat
     1021 tgggctatag ccaagcggta aggcaacgga ctttgactcc gtcactcgtt ggttcgaatc
     1081 cagctagccc agccattaga gccattagct cagttggtag agcatctgac ttttaatcag
     1141 agggtcagag gttcgaatcc tctatggctc actacttgca ctttccattc ttgggaagtg
     1201 ctttttttat tttttgagaa ttaggatgct aaggcgttat gttatggcta ttacttgatt
     1261 atagtaagcg tatacattat tctgaaatta tgtataacaa agggggacgc ttatgacaaa
     1321 gaaaaaagga attgatatta ttggtgtacc gtcaacattt ggacaaagaa aattaggtgt
     1381 tgatttagga ccaactgcga ttagatatgc aggtttaata ccaagactaa agcaattaga
     1441 ccttgatgtt aatgacaaag gggatattaa ggtatcagct gtagacattg aaaaatttca
     1501 aagtgaacaa aatggattaa gaaactacga tgaaattata gatgttactc aaaaattaaa
     1561 taaagaagta tcagcatcaa ttgaaaataa cagattccct ctagttcttg gtggagatca
     1621 ttctattgcg gtaggttcag tatcagcaat aagtaaacat tataataatt taggtgttat
     1681 ttggtatgat gcacatggtg atttaaatat acctgaagag tcaccaagtg gaaatattca
     1741 tggtatgcct ctaaggattt tgacaggtga aggtcctaaa gaacttttag aattaaatag
     1801 taatgtaatc aagccagaaa acatcgtact aattggtatg agagatttag ataaaggtga
     1861 aagacaattt atcaaagatc ataatattaa aacatttact atgtcagata ttgataaatt
     1921 ggggataaag gaagtaattg aaaatacaat agaatatttg aagtcacgca atgttgatgg
     1981 cgttcattta tctttagatg ttgatgcctt agatccactt gaaacgccag gcacaggtac
     2041 tagagtctta ggcggactta catatagaga aagtcatttt gcgctggaat tattacatca
     2101 gtctcagtca gttacttcaa tggacttagt tgaagtgaat ccacttattg atcataataa
     2161 ccatactgct gagcaggcag tttcattaat tggaacattt tttggtgaaa ctttattata
     2221 aataaatgat ttgtagtgta taaagtatat tttgcttttt gcactacttt ttttaattca
     2281 ctaaaatgat taagagtagt tataatcttt aaaataaatt ttttctattt aaatatatgt
     2341 tcgtatgaca gtgatgtaaa tgattggtat aatgggtatt atggaaaaat attacccgga
     2401 ggagatgtta tggatttttc caactttttt caaaacctca gtacgttaaa aattgtaacg
     2461 agtatccttg atttactgat agtttggtat gtactttatc ttctcatcac ggtctttaag
     2521 ggaactaaag cgatacaatt acttaaaggg atattagtaa ttgttattgg tcagcagata
     2581 agtatgatat tgaacttgac tgcaacatct aaattattcg atatcgttat tcaatggggg
     2641 gtattagctt taatagtaat attccaacca gaaattagac gtgcgttaga acaacttggt
     2701 agaggtagct ttttaaaacg ctatacttct aatacgtata gtaaagatga agagaaattg
     2761 attcaatcgg tttcaaaggc tgtgcaatat atggctaaaa gacgtatagg tgcattaatt
     2821 gtctttgaaa aagaaacagg tcttcaagat tatattgaaa caggtattgc aatggattca
     2881 aatatttcac aagaactttt aattaatgtc tttataccta acacaccttt acatgatggt
     2941 gcaatgatta ttcaaggcac taagattgca gcagcagcaa gttatttgcc attgtctgat
     3001 agtcctaaga tatctaaaag tttggggaca agacatagag ctgcggttgg tatttcagaa
     3061 gtatctgatg catttaccgt tattgtatct gaagaaactg gtgatatttc ggtaacattt
     3121 gatggaaaat tacgccgaga catttcaaac gaaatttttg aagagttgct tgctgaacat
     3181 tggtttggca cacgctttca aaagaaaggt gtgaaataat atgctagaaa gtaaatgggg
     3241 cttaagattt attgcctttc ttttggcatt gtttttcttt ttatctgtta acaatgtttt
     3301 tggaaatatc tttaacactg gtaatcttgg tcaaaagtct agtaaaacga ttcaagatgt
     3361 acccgttgaa attctttata acactaaaga tttgcattta acaaaagcgc ctgaaacagt
     3421 taatgtgact atttcaggac cacaatcaaa gataataaaa attgaaaatc cagaagattt
     3481 aagagtagtg attgatttat caaatgctaa agctggaaag tatcaagaga agtatcaagt
     3541 taaagggtta gctgatgaca ttcattattc tgtaaaacct aaattagcaa atattacgct
     3601 tgaaaacaaa gtaactaaaa agatgacagt tcaacctgat gtaagtcaga gtgatattga
     3661 tccactttat aaaattacaa agcaagaagt ttcaccacaa acagttaaag taacaggtgg
     3721 agaagaacaa ttgaatgata tcgcttattt aaaagccact tttaaaacta ataaaaagat
     3781 taatggtgac acaaaagatg tcgcagaagt aacggctttt gataaaaaac tgaataaatt
     3841 aaatgtatcg attcaaccta atgaagtgaa tttacaagtt aaagtagagc cttttagcaa
     3901 aaaggttaaa gtaaatgtta aacagaaagg tagtttagca gatgataaag agttaagttc
     3961 gattgattta gaagataaag aaattgaaat cttcggtagt cgagatgact tacaaaatat
     4021 aagcgaagtt gatgcagaag tagatttaga tggtatttca gaatcaactg aaaagactgt
     4081 aaaaatcaat ttaccagaac atgtcactaa agcacaacca agtgaaacga aggcttatat
     4141 aaatgtaaaa taaatagcta aattaaagga gagtaaacaa tgggaaaata ttttggtaca
     4201 gacggagtaa gaggtgtcgc aaaccaagaa ctaacacctg aattggcatt taaattagga
     4261 agatacggtg gctatgttct agcacataat aaaggtgaaa aacacccacg tgtacttgta
     4321 ggtcgcgata ctagagtttc aggtgaaatg ttagagtcag cattaatagc tggtttgatt
     4381 tcaattggtg cagaagtgat gcgattaggt attatttcaa caccaggtgt tgcatattta
     4441 acacgcgata tgggtgcaga gttaggtgta atgatatcag cctctcataa tccagttgca
     4501 gataatggta ttaaattctt tggatcagat ggttttaaac tatcagatga acaagaaaat
     4561 gaaattgaag cattattgga tcaagaaaac ccagaattac caagaccagt tggcaatgat
     4621 attgtacatt attcagatta ctttgaaggg gcacaaaaat atttgagcta tttaaaatca
     4681 acagtagatg ttaactttga aggtttgaaa attgttttag atggtgcaaa tggttcaaca
     4741 tcatcactag cgccattctt atttggtgat ttagaggcag atactgaaac aattggatgt
     4801 agtcctgatg gatataatat caatgagaaa tgtggctcta cacatcctga aaaattagca
     4861 gaaaaagttg ttgaaactga aagtgatttt gggttagcat ttgacggaga tggagacaga
     4921 atcatagcag tagatgagaa tggtcaaatc gttgacggtg accaaattat gtttattatt
     4981 ggtcaagaaa tgcataaaaa tcaagaattg aataatgaca tgattgtttc tactgttatg
     5041 agtaacttag gtttttacaa agcgcttgaa caagaaggaa ttaaatctaa taaaactaaa
     5101 gttggagaca gatatgtagt agaagaaatg cgtcgcggta attataactt aggtggagaa
     5161 caatctggac atatcgttat gatggattac aatacaactg gtgatggttt attaactggt
     5221 attcaattag cttctgtaat aaaaatgact ggtaaatcac taagtgaatt agctggacaa
     5281 atgaaaaaat atccacaatc attaattaac gtacgcgtaa cagataaata tcgtgttgaa
     5341 gaaaatgttg acgttaaaga agttatgact aaagtagaag tagaaatgaa tggagaaggt
     5401 cgaattttag taagaccttc tggaacagaa ccattagttc gtgtcatggt tgaagcagca
     5461 actgatgaag atgctgaaag atttgcacaa caaatagctg atgtggttca agataaaatg
     5521 ggattagata aataaatact gtattacaaa tgagccgatg cgtatgcatc ggttttttaa
     5581 tttgattaaa gcaagaatat tatttcgtaa agacaaaata ataaagaaca cataaaaaca
     5641 aagtaatcaa tatgtaatat aaaattcact ggtaagcaat atataatgat gataaaatta
     5701 attttaatta gatagagttg ctctgacttt tataagatgg aagcaaactg tttatcttaa
     5761 aagttgatta agtgacatca tttaacagcg agaataatca accaggagga tgacttaatg
     5821 aatttattca gacaacaaaa atttagtatt agaaaattta atgtcggtat tttttcagct
     5881 ttaattgcca ctgttacatt tatatctatt aacccgacaa cagcgtctgc agcagagcaa
     5941 aatcagcctg cacaaaatca accagcacaa ccagctgatg ccagtgcaca gcctaacgca
     6001 aatgctggtg ctcaagctaa tcccacagca cagccagctg cacctgccaa ccaaggacaa
     6061 ccagcagcac aacctgcaaa ccaaggtgga caggctaatc cagcaggagg cgcagcacaa
     6121 ccggcagcag gtacaacaca accagctgga caaggtaatc aagctgatcc gaataacgct
     6181 gcacaagcac aacctggaaa ccaagcaggt caaggaaata accaagcaac acctaataat
     6241 aatgcaacac cggcaaatca aacacagcca gcgaatgctc cagcagcagc gcaaccagca
     6301 gcacctgtag cagcaaacgc acaaactcaa gatccaaatg ctagcaatac tggtgaaggc
     6361 agtattaata caacattaac gtttgatgac cctgccattt caacaaatga taacagacag
     6421 gatccaactg taacggttac agatcatgta aatggttatt cattaattaa caatggtaag
     6481 attggtttcg ttaactccga attaagacga agcgatatgt ttgataagaa taaccctcaa
     6541 aactaccaag ctagagggaa tgtagctgca ttaggtcgtg tgaatgcaaa tgattctaca
     6601 gatcatggta actttaacgg tatttcaaaa actgtaaacg taaaaccaga ttcagaacta
     6661 attattaact ttactactat gcaaacgaat agtaagcaag gtgcaacaaa tttagttatt
     6721 aaagatgcta agaaaaatac tgaattagca actgtaaatg tagctaagac tggtactgca
     6781 cacttattta aagtaccaac agatgctgat cgtttagatt tacaatttat tcctgacaat
     6841 acagcagttg ctgatgcttc aagaattaca acaaataaag atggttataa atactattca
     6901 ttcattgata atgtaggtct attctcagga tcgcatttat atgtcaaaaa tagagattta
     6961 gcaccgaaag caactaacaa taaagaatat actattaata ctgaaatcgg taacaatggt
     7021 aattttggtg cttcattgaa agcagatcaa tttaaatatg aagtaacatt accacaaggt
     7081 gtaacttacg ttaatgattc attaactaca acattcccta atggtaatga agacagtaca
     7141 gtattgaaaa atatgactgt caattatgat caaaatgcaa ataaagttac atttacaagc
     7201 caaggtgtga caacagcacg tggtacacat acgaaagaag ttttattccc agataaatct
     7261 ttaaaattat catataaagt taatgttgcg aatattgata cacctaaaaa tattgatttt
     7321 aatgaaaaat taacatatcg tactgcttca gatgttgtaa ttaataatgc gcaaccagaa
     7381 gttacactaa ctgcagatcc attttcagta gcggttgaaa tgaacaaaga tgcgttgcaa
     7441 caacaagtta actcacaagt tgatgatagt cattacacaa cagcatcaat tgcagaatac
     7501 aataaactta aacaacaagc agacactatt ttaaatgaag atgcgaatca tgttgaaact
     7561 gcaaatcgtg catctcaagc ggatattgat ggtttagtaa ctaaattaca agctgcatta
     7621 attgataatc aagcagcaat tgctgaatta gatgctaaag ctcaagaaaa ggttacagca
     7681 gcacaacaaa gtaaaaaagt tacgcaagat gaagttgcag cgcttgtgac taaaattaac
     7741 aatgataaaa ataatgcaat cgcagaaatt aataaacaaa caacagcaca aggtgtcaca
     7801 actgaaaaag ataatggtat cgcagtgttg gaacaagatg tgattacacc aacagttaaa
     7861 cctcaagcga aacaagatat tatccaagca gttacaactc gtaaacaaca aattaaaaag
     7921 tcaaatgcat cattacaaga tgaaaaagat gtagcaaatg ataaaattgg taaaattgaa
     7981 acaaaggcaa ttaaagatat tgatgcagca acaacaaatg cacaagtaga agccattaaa
     8041 acaaaagcaa tcaatgatat taatcaaact gcacctgcta caacagctaa agcagcagct
     8101 cttgaagaat ttgacgaagt tgttcaagca caaattgatc aagcaccttt aaatcctgac
     8161 acaacaaatg aagaagtagc ggaagctatt gagcgtatta atgcagctaa agtttctggt
     8221 gttaaagcaa ttgaagcgac aacgactgca caagatttag aaagagttaa aaacgaagaa
     8281 atattcaaaa ttgagaatat tacggactct acgcaaacta aaatggatgc ttataaagaa
     8341 gtgaaacaag ctgccacagc tagaaaagcg caaaatgcta cagtttcaaa tgcaactgat
     8401 gaagaagtag cagaagctga tgcagcggta gacgcagctc aaacagaagg tctacatgac
     8461 atccaagttg ttaaatcaca acaagaagtg gctgatacta aagcaaaagt attagataaa
     8521 atcaatgcca ttcaaacaca agcaagagtt aaacctgcag cagatacaga agtagaaaac
     8581 gcatataata cacgtaaaca agaaattcaa aatagcaatg cttcaactac agaagaaaaa
     8641 caagctgcat atacagaatt agatactaaa aagcaagaag caagaacaaa ccttgataca
     8701 gcaaattcaa atagtgaagt tgcaacagct aaagacaata gcattgctgc aattaatcaa
     8761 gttcaagctg ccacaactaa gaaatcggat gcaaaagcgg aaatcgctca aaaagcaagt
     8821 gaacgtaaaa cagcaatcga agcaatgaat gattcgacta ctgaagaaca acaagcagcg
     8881 aaagataaag tggatcaagc agtagttaat gcaaacgctg atattgataa tgccgcagca
     8941 aacaatgatg tggataatgc aaaaactaca aatgaagcta caatcgcagc cattacacct
     9001 gatgcaaatg ttaaacctca agcgaaacaa gcaattgcag ataaagtaca agcgcaagaa
     9061 acagcaattg atgctaataa cggtgcaaca acagaagaga aaacagcagc gaaacaacaa
     9121 gttcaaacag aaaaaacgac agctgatgca gcaatagatg gtgcacattc aaatgcagaa
     9181 gttgaagcgg ctaaaaatgc agaaattgct aaaattgaag caattcaacc agcgacaaca
     9241 acgaaagatg atgcgaaaca agccattgct acgaaagcga atgaacgtaa agcagccatt
     9301 gctcaaacgc aagagattac agcagaagaa attgcagcgg ctaatgcgaa cgtagataat
     9361 gcagtaacag aagcaaacaa ccacattgag actgctaata gtcaaaatga agttgatcaa
     9421 gcgaagacaa caggtgaagc tagtattgat caagtgacac caactgttaa taaaaaagct
     9481 actgcacgta atgaaatcac tacaatatta aataacaagt tacaagcaat tcaagctacg
     9541 ccagatgcaa cagatgaaga gaaacaagca gcagaaactg aagctaatac tgaaaatgct
     9601 aaagcaaatc aagccatcac agcagctaca acaaatgcag aagtagatga ggcaaaaact
     9661 aatgcagaag cggcaattaa cgctgtaaca ccaaaagtta tgaagaaaca agcagcgaaa
     9721 gatgagatcg atcaattaca agcagcacaa acagcggtta ttaataacga ccaaaacgca
     9781 acaaatgaag aaaaagaagc ggcgattcaa caactagcaa cagcagttac agacgcgaaa
     9841 aataacatta cagctgcaac tgacaataat ggtgtagaca ctgctaaaga tgcaggtaaa
     9901 aattcgattc aaagtacaca accagcaaca gcagttaaat cgaatgcgaa aaatgaagta
     9961 gatcaagctg tgacaactca aaatcaagca attgataata ctacaggtgc aacgactgaa
    10021 gagaaaaatg cagcaaaaga tttagtttct aaagctaaag aaaaagcata tcaagatatt
    10081 ttaaatgcgc aaacgactaa cgatgttaca caaattaaag atcaagcagt tgctgacatt
    10141 caaggtatta ctgctgatac gacaattaaa gatgtagcta aagatgaatt agcaacaaaa
    10201 gcaaaggaac aaaaagcact tattgcacaa actgcagatg cgactactga agaaaaagaa
    10261 caagcaaatc aacaagtaga tgcacaatta acgcaaggta atcaaaatat tgaaaatgca
    10321 caatcaatcg atgatgtaaa cactgcaaaa gataatgcaa ttcaagccat cgacccaatt
    10381 caagcatcaa cagatgttaa aacgaatgca agagcggaat tgctaaatga aatgcaaaat
    10441 aaaataactg aaatccttaa tgataatacg actacaaatg aagaaaaagg aaaagatata
    10501 gaaccagtta gagcagcata tgaagaaggt ttaaataata ttaatgcagc aactactaca
    10561 ggtgatgtaa ctactgctaa agatacagca gtacaaaaag ttcaacaact tcatgcaaat
    10621 cctgttaaga aaccagcagg taaaacagaa ttagatcaag ctgcagctga taagaaaaca
    10681 caaattgaac aaacaccaaa tgcgtcacaa caagaaatta atgatgcaaa acaagaagtt
    10741 gatgcagcgt taaatcaagc gaaaacaaat attgatcaat catcaacaaa tgaatatgtt
    10801 gataatgcgg ttaaagaagg gaaagctaaa attaatgcag ttaaaacatt tagtgagtac
    10861 aaaaaagatg ctttagctaa aattgcagat gcatataacg ctaaagtaaa cgaagcggat
    10921 aactctaacg catcgacttc aagtgaaatt gctgaagcga aacaaaaact tgctgaatta
    10981 aaacaaacag cggatcaaaa tgttaatcaa gctacttcta aagatgacat tgaagttcaa
    11041 atccataatg acttagataa tattaacgat tacacaattc caaccggtaa aaaagaatcg
    11101 gctacaacag atttatatgc ctatgcagat cagaagaaaa ataatatttt agctgacact
    11161 aatgcaacac aagatgaaaa acaacaagca attaagcaag ttgaccaaaa tgttcaaact
    11221 gcattagaaa gcattaataa tggtgtggat aatagtgatg ttgatgatgc attaacacaa
    11281 ggtaaagcag caattgatgc tattcaagta gatgctactg ttaaacctaa agcgaaccaa
    11341 gcaattgatg ataaagcaca ggaaacgaaa gagtctatag accaaagtga ccagttaact
    11401 gctgaagaaa aaacggctgc attagcaacg attaaacaaa ttacagatca agctaaacaa
    11461 ggtattactg atgcaacaac aactgctgaa gttgaaaaag cgaaagctca aggacttgag
    11521 gcgtttgata acattcaaat tgactcaaca gaaaaacaaa aagctatcga agaattagaa
    11581 acttcactag accagattga agcaggagta aatgtcaacg ttgatgctac aactgaagaa
    11641 aaagaagcgt ttacgaatgc attagaagac attttatcaa aagcaactga agatatttct
    11701 gatcaaacta caaatgcaga aatcgctact gttaaaaata gtgcgcttga acaacttaaa
    11761 gcacaacgta ttaatccagt agttaagaaa aatgctttgg aagcaattag agaagtggtt
    11821 aacaagcaaa tagaaataat taaaaatgca gatgcagatg catcggcgaa agaaattgca
    11881 cgtacggatt taggtagata ttttgacaga tttgctgata aattagataa aacacaaaca
    11941 aatgcagaag ttgatgaatt acaaaatgta acgatacctg caattgaagc gattgttcct
    12001 cagaaggatc caaatgctaa tgatactaat agtggttcag ataataatga tgcaacagca
    12061 aattcaaatg ctaatgcaac accagaaaat actggacaac ctaatgtaac tgagtcaaca
    12121 gataatgcca atgcagatac gtcttcaaca acgacaaata atcagaatga tgcggcaact
    12181 ggtggaacaa cagcaacatc aacaaacaat tctagtgtag atgcatcaac agacaattcg
    12241 gcaacgggta atggtacaac tgataaacct gcaacagaga cagataatgc aacgccagca
    12301 gaaggcacaa gaaataacaa tggtacggca actgcaacaa acgagaacgc accaacagga
    12361 tcaacagcaa cggctccaac aacaaatgcg agcacaggtg cagcatcatc tgctgatagc
    12421 aaagataatg catctgtaaa tgattcaaaa caaaatgctg aagtaaataa tagtgctgaa
    12481 tctcaatcaa ctaatggcaa ggttgcacaa ccaaaatctg aaaataaagc taaggcagaa
    12541 aaagatggta gagattcaac aaatcaaagt atggttgaat caacaactga aacattgcct
    12601 tcagcagaca ttacaggacc aaaagtgcct tctaatacag agaaagataa agaagaaagt
    12661 acgacaaatc aaacagatgc tggacaactt aagtctgaaa ctaacgttgc atctaacgaa
    12721 gcagataaat ctgaaggcaa cgttgatact gatgtatcga ataaaccatc aacatctgca
    12781 tcttctgaag caaaagaaaa aatgacttca attaatgtta gccaaaaaga tgatacggca
    12841 acagcagata ctaatgatac gcaaacatca gttggcccag ttgcaaacaa taaagctacg
    12901 caaaacgacg gtaccaatgc atctccagct acagtttcaa atggaagcaa tagtgctaat
    12961 caagatatgc ttaatgtaac taacactgaa gaaaacaaag caaatgcaaa atcagctcaa
    13021 caaggaaaag ttaataagct taaacaacaa gctaaaactt taccagatac tggtatgtca
    13081 cataatgacg atttaccata tgctgaatta gctctaggtg caggtatggc attcttaatt
    13141 agaagattca ctaagaaaga ccaacaaact gaagaataaa atttatttaa gaatgtgaac
    13201 atctagttat ttcataggga aatattcctt actaagtaac caagtctaac agttaatatg
    13261 aatttgtttt agtaatgacc aatagccatt gaataggtgg ctgtggctcg aaaataaaac
    13321 aaattattaa cgcaaaaata ctcctttcat acataacaca agttaagtgt ctatgttgaa
    13381 gggagtattt tttatgttca gaaatttaat attgttaatt caacggaact actacattat
    13441 tactgattgt taacgaataa cgttggaaaa ttatagtttc aaacaaagct caataaatta
    13501 tgaaagtgaa ttgtacgttt caattatttc atcggctaag ccatcgtcaa ctttagcata
    13561 ttcgcttaag aacgctttta aaccgtgttc tttaatgtaa ttatttttct caacagtttc
    13621 ttggtctgct gtatcatcat acttcaataa caatgcagct gtatttaata aaccagtgcg
    13681 ttctaaatct ttattataaa gatatgttaa tggttttata attctatctt tagggccaat
    13741 tttacgtaac gtacctcgtc cgacacgtgt tacttcatca gataaataag aattgttgaa
    13801 acggtctatt attttttcaa cataaccagc ttgctccgct tcagtaaaat caaattcatt
    13861 agtaatatat tgactagttt cagctaaaac acggcgtaag ccagcttcaa ttgaactatc
    13921 ttttacagcg tctaatacgg tagctttacc tgcaaattta ccagcatacg ctaaatatgc
    13981 atgtcctgta ttcacagtta ataatttacg ctcaatatat ggtgttaaat catcaacata
    14041 tttaatatga tttaattctg gaccatacca tgcatctttt tcaacaaccc attcgtaaaa
    14101 tggctcaacc ataacgtcta atatattttc attcttttgt aatggtacaa ttctatcaac
    14161 tgctgagtta gcaaaatgaa tattgttacc aagagggcca gtaatatcaa gtaccgcttt
    14221 tttcaatgta tcagttgcca taatagcatt ctcacaagca acaatattaa catggtttgt
    14281 tttttctttt aagaaaggcg caaaagattt agcaataata ggtagtatgt taacaccaac
    14341 agctgttgta ataatatcag cttctagaat tgcttgtttc aatgcttcag aaggttgcat
    14401 tgaattaatt gcatcaacat tattcacgcg cgtcgttgtt ttagactcat cagctaaaat
    14461 aacatcgtat tgatgatcat gagctaaagc attaatgatt tcttcattga cgtctgcaaa
    14521 tgttacttta acattgttgt ctgcaagaat ataaccaatg aaaccacgac ctatgttacc
    14581 agcaccaaag tgaactgctt tcattatgca tctgcctcct cgaatacttg tttaatttct
    14641 tctgcagatt ttgcttgaac gatacgatcc acattttctt cttcgctaaa tgtaattgca
    14701 attttagata acaagtctaa atgttcgcca tctttacctg cgattcccac gacaacttta
    14761 actacttcgc catcccagtc aacgccttca gggatttgta ataatgttaa acctgagtga
    14821 atcacatttg ttttagcttc atctgtacca tgaggaattg ctaagccatt tcccataaat
    14881 gttgatacga cttgctcacg atctttcatt gcttgaatat aagcatctgt tacagcacca
    14941 ctatcaacta aggctttacc tgctttttca attgcttcat tttggctgtt aacatttaca
    15001 tttaaaaaga tattgtcatt actaaataat tcgctcattc tgtacactcc taaataaatt
    15061 gttttttaat tcgttctaaa aatttgtttc ttaataatgt ttcaagttcc tgtggatttt
    15121 ccataaaagt atcaatagat tctaatttca aacttaaaaa ttcggacaag tcacttatca
    15181 gtgatgccat aaaatcattg tcaggaataa acatggaaat catatattta attgtttgat
    15241 tgtcattttg aatactaggc aatgttaacg gttcttctaa aactgtgatt agaatcatag
    15301 ggtgtttaat catattgtct cttaggtgtg gtattgcaac aggatatgga cttaatatcc
    15361 atcctggatt gtcgatcaat cgttgctcta gtagttcagc gaatgacgag ggatcgttta
    15421 ttacatcgca ttgatataga taatctgcga tatatgtttg ccactgttta acttcggtac
    15481 taactatttt cactgaatca agcaaagtta aacccgaacg catatgatgc attaattgtt
    15541 ctgatttttc gtcacttaaa tgtttttcga aaactgaagt taagtttgaa gtttgttcat
    15601 tttcaaaaga tgtgttgtca tcaatatctt tcgtttcaac atgaacatca tccttatcaa
    15661 tcatgtttga tgatttatca tgcgtctttt ggaacgtaga ctttgtattt aaaaactgtg
    15721 cgacataact gatatcacta tctggtaata atggatttac cgttaaatag ggggagtcga
    15781 tgtctaaatt cacagtagaa ataatgccat catattgact taaatctagt gacttcaaat
    15841 cgctgactga tgcttgtgta atacgctcaa tctcactaaa aacttgctct agacgagttg
    15901 ataatagacg gctagttccc ataccactgc tgcaaacgac taatatgttt aaaaatcgat
    15961 taccttggtt tttaatcgag ccaccaaagt gtaaaactat aaaagcaatt tcactctctg
    16021 gaaaaatcaa atcaggccaa caatcattta aagctaatct tacattttca aacagtcttg
    16081 gatatttgaa cttaatcatg tctgttaacg gattataggt ctcgatatta gcttgcaaac
    16141 gattgattgc aggattaagg tgcagcgtca gtccatctac taaagtatcc aaatctgcaa
    16201 atgtcatacc tgaaatcatt tctacttttt taacaaacgc tttaattttg ttttcttcac
    16261 aacgattatt taatgatgta tcattaagat tttttcgttt agcaccacgt aaatggatag
    16321 taatgaaagc aatttctgcc tgattaaacg tgacgtcata tatttgacca agtttatcag
    16381 caagttcgct tgctactttg tgttcaaatg tgttttgaac agactcataa atatcatcgt
    16441 ttaatgcgac atactctcca ttttttatac gtgaaatgga tagaacgata tggacagtta
    16501 aagttaaata acttgatttg gtaatttggt atggtaaggc acttaggtag tccattaata
    16561 gtctttcaac attaaaaata cgctctaagt caacaaagtc tttgtggatt tgatttaatg
    16621 aatgaaagac aaaatgattt tcaattactg aataaacgct agtactattt aagttattca
    16681 ccattaattg acttaaaaat tcacgtttct ttgattcagt acctaccaag taaatacctt
    16741 cgccacgctt tcgagataaa gatagttggt acttatttaa atcgagctct aaatcatcta
    16801 acatctttgc taaagtctgg acagaaacgc caatttcttg cgctaaacta tattgtttaa
    16861 ctggctcctt ggcttgtatc aaagcgtata taataattac tttctgctct tcaacagata
    16921 agtcaatggt ttgatgttgt gcgatcgatt gcttcaaatc gtttaaatca gaatctgcgc
    16981 cagcaatgcg tagccctttt ttgtttgcgc gttctaaagt taatgaaaat gaagttaaat
    17041 atgcttcaac accttttaat tcacggtgaa tagtacgaga ggacaccgca agttgctgag
    17101 caatgtcgtg tatagtgata tattgaccgt ggtacttaat caatagggct atcatttctt
    17161 tttcacgtat actcaataac atggcggtat cctccatttt ttaatttatt taattattat
    17221 gcttgatcat ctttttttag attatttaaa agttcttcat atcttggtga attaaggaaa
    17281 ttatccactg aaatatggat ggcatttggt gtttgtttaa ttgcacgatc agttagtttt
    17341 ttctgagtga taactaattg agcatctttt ggcaattgat taattgcagt atttgtaact
    17401 gtaatatcat taatgcctgc ttttttaaat ttattacgta acatgcttgc acccattgca
    17461 ctagaaccca tacccgcatc gcaggcaaaa ataacatgat ttatattatt gtaattgtgt
    17521 gcatcaacat cttcagtgtt gtaattatcc aatagcgctt caggatcatc atctaatgaa
    17581 gatgtttcac taacattacc actagcattt tcctctttat taacattttt atcagaagat
    17641 actaacttag aagcaacgct tgattttttc cctttagtag tttccatttg agcagttgca
    17701 gcttctaaat cttgtttagg ttctttagtg aacttcataa ttaaagcagc tacaacaaat
    17761 gatacaagtg cagcaaggaa gacaccgagt aacatgtgca agaattcacc tctaggtgca
    17821 tttaaacagt aaactataaa tgaaccaggt gacgcgggac ttttaaatcc aaatcctgtt
    17881 gcttgataag ttgcaacacc agtcattccg cctaaaataa cagcgatgaa taataaagga
    17941 cgcattaata catatgggaa ataaatttca tgaataccac cgaagaagtg gataattcca
    18001 gcaccatatg aagttgcttt tgcagtacct ttcccaaaaa tcatatatgc aagtaaaatt
    18061 cctaaacctg gtccagggtt agattcaatt gtgtataaaa tagattgacc agcttttgca
    18121 gcttgatctg caccaagcgg tgtgaataca ccatggttaa tcgcattgtt taaaaataca
    18181 atttttgcag gctctactaa aatacttaca agtggaagta ggtgtgcatg tactaatgct
    18241 tcaactgcca ctgataaaat atgcataata aatttcataa gtggtgctaa aattttaaat
    18301 cctgcaattg tcatgataaa tcctaaaata ccagctgaaa aattattaaa tagcatttca
    18361 aaaccttgcg gcgttctagg ttgaatcaat tggtcggtct tcttcattaa ccaaccaaca
    18421 agtggaccca taatcattgc accaagtaac attggtgtat caggtaatgc aacgatgacc
    18481 cccatagttg ccgttgcagc gataatacca ccacgtaaat cataaattaa acgaccgcca
    18541 ctaaatgcaa tcaatagtgg gattaaataa gtaatcattg gaccagccaa cgtagctaaa
    18601 tctttattcg ggaaccaacc attatctata aaaatggccg cgatgaaacc ccaagcgatg
    18661 aatgcaccaa tgtttggcat aatcatacta ctcaaaaatg atccgaatgc ttgaacgcga
    18721 cgaccaattc cttttttctc ttcagtttgc gacatattta cacctctttt ttaatgtatg
    18781 tgactaccat tagtgtaaag agacatacct tattactcaa tataaacaaa aatcaacttt
    18841 gtcaaaataa atgtgacaaa attaaataaa gtgtcataaa tgtgacggta tagatatttt
    18901 gaaaaagtaa aacaaaaaaa ttgttttagg atttttaaag ttttattgtg aaaatatttg
    18961 caaaacaaaa caacaccgtt gtacaataat gattaatgga aagggggaaa gttcggcagt
    19021 acagttaaag cgcctgtgca aataaatatt tgtatttgaa gattaaaggt taatatatga
    19081 gtggccttta tagagtgcaa tatatgtatt tgtagacgag gaggatagtg atcgaataga
    19141 tcggcggatg ctatcccgga tgtggctcat tcgttagctt attaagtaaa acattagggt
    19201 gacttaatgg acaaagttaa taagatcgcc agaaattgaa tataaaaaat attaatatgg
    19261 aaagtacagt gtgagcaatt tgtatagttg taaaaataac tatgcttaat ttgttatgga
    19321 tgaatgcgat gatagcatgt tcctatttat attatgaaag cagattgtca atctaaatta
    19381 tcggcaataa atcataattt acgcgtacta ttccaatatg gaggaaaatg tcgttatgtg
    19441 tggaattgtt ggttatattg gttatgataa tgccaaagaa ttattattaa aaggtttaga
    19501 aaaattagaa tacagaggtt atgactctgc aggtatcgca gtagtaaatg atgataatac
    19561 aactgtattc aaagaaaaag gtcgtattgc agaattacgt aaagttgctg atagtagcga
    19621 ttttgatgga cctgttggaa tcggtcacac acgttgggca acacacggtg taccgaatca
    19681 tgaaaactct catccacatc aatcatcaaa tggccgtttt actctagttc ataacggtgt
    19741 tattgaaaac tatgaagagt taaaaggtga atacttacaa ggtgtatcat tcatttcaga
    19801 aacagataca gaagttatcg ttcaattagt tgaatacttt tcaaatcaag gactttcaac
    19861 tgaagaagca tttacaaaag ttgtgtcatt attacatggt tcatatgcat taggtttatt
    19921 agatgctgaa gacaaagaca caatctatgt tgctaaaaat aaatcaccat tattattagg
    19981 tgttggtgaa ggtttcaatg ttatcgcatc agacgcactt gcaatgttac aagtgacaag
    20041 cgaatataaa gaaatccatg accatgaaat cgttattgtt aaaaaagatg aagttattat
    20101 taaaggtgca gatggaaacg ttgtagaacg tgattcatat attgctgaaa ttgatgcatc
    20161 agatgctgaa aaaggtgttt atgcacacta catgttaaaa gaaattcatg aacaaccagc
    20221 agtaatgcgt cgtattattc aagaatatca agatgcagaa ggtaacttga aaattgatcg
    20281 agacatcatc aatgatgtta aagaagcaga ccgcatttac gttattgcag caggtacaag
    20341 ctaccatgca ggtttagtag gtaaagaatt tttagaaaaa tgggctggcg taccaactga
    20401 agtacacgtt gcatcagagt ttgtctacaa catgccatta ttatctgaaa aacctttgtt
    20461 cgtttatatt tctcaatcag gtgaaactgc agatagccgc gccgtattag ttgaaactaa
    20521 taaattaggt cataaatcat taacaatcac taatgttgca ggttcaactt tatcacgtga
    20581 agcagaccac acattgttat tacacgcggg tcctgaaatc gcagttgcat ctacaaaagc
    20641 atatactgca caaattgcag tattatcaat cttgtctcaa atcgttgcaa aagagcacgg
    20701 tcgtgaagca gatattgatt tattgagaga attagcaaaa gtaacaacag caattgaagc
    20761 gattgttgac gatgcaccaa tcatggaaca aattgctaca gatttcttag aaacaacacg
    20821 caatgcattc tttatcggac gtactattga ctataacgta agtttagaag gtgcgttaaa
    20881 acttaaagaa atctcttaca ttcaagctga aggttttgca ggtggagaac ttaaacacgg
    20941 tacaattgcc ttaatcgaag aaggtacacc agttgtaggt ttagcaacac aagagaaagt
    21001 taatttatca attcgtggta atgttaaaga ggtagtagca cgtggagcac acccatgtat
    21061 tatttctatg gagggtcttg aaaaagaagg cgacacttat gtcattcctc atgtacatga
    21121 attattaacg ccattagtat cagttgttac attacaatta atttcgtact atgcagcatt
    21181 acacagagat ttagatgtgg ataaaccacg taaccttgct aaatctgtta ctgtggaata
    21241 attcactttt ttatcagtgc atgttgtagg tttgaaataa agttgtaccg ttgataaaaa
    21301 agatgtaccg ttttaaataa aattataccg tttataaaaa agttgcaccg ttttatgagt
    21361 ctgggacata aatccctaaa aaacagcagt a
//
LOCUS       DAPIDM010000031        21221 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000031 DAPIDM010000000
VERSION     DAPIDM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.031"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            46..570
                     /locus_tag="RJE42_001047"
     CDS             46..570
                     /locus_tag="RJE42_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDV5876479.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQFNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            597..1835
                     /locus_tag="RJE42_001048"
     CDS             597..1835
                     /locus_tag="RJE42_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV5876480.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKMYQQQVVKNAKVLAETLINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            1863..2492
                     /gene="upp"
                     /locus_tag="RJE42_001049"
     CDS             1863..2492
                     /gene="upp"
                     /locus_tag="RJE42_001049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV5876481.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            2516..3643
                     /gene="wecB"
                     /locus_tag="RJE42_001050"
     CDS             2516..3643
                     /gene="wecB"
                     /locus_tag="RJE42_001050"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV5876482.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLNE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENLGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVRDVAHKILGGHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            3711..4163
                     /locus_tag="RJE42_001051"
     CDS             3711..4163
                     /locus_tag="RJE42_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDV5876483.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKSHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            4184..4912
                     /gene="atpB"
                     /locus_tag="RJE42_001052"
     CDS             4184..4912
                     /gene="atpB"
                     /locus_tag="RJE42_001052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV5876484.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            4955..5167
                     /gene="atpE"
                     /locus_tag="RJE42_001053"
     CDS             4955..5167
                     /gene="atpE"
                     /locus_tag="RJE42_001053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV5876485.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            5365..5886
                     /locus_tag="RJE42_001054"
     CDS             5365..5886
                     /locus_tag="RJE42_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV5876486.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            5886..6425
                     /locus_tag="RJE42_001055"
     CDS             5886..6425
                     /locus_tag="RJE42_001055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV5876487.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            6447..7955
                     /locus_tag="RJE42_001056"
     CDS             6447..7955
                     /locus_tag="RJE42_001056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV5876488.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            7986..8852
                     /locus_tag="RJE42_001057"
     CDS             7986..8852
                     /locus_tag="RJE42_001057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV5876489.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            8874..10286
                     /gene="atpD"
                     /locus_tag="RJE42_001058"
     CDS             8874..10286
                     /gene="atpD"
                     /locus_tag="RJE42_001058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV5876490.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTLQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            10306..10710
                     /locus_tag="RJE42_001059"
     CDS             10306..10710
                     /locus_tag="RJE42_001059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV5876491.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            11363..11596
                     /locus_tag="RJE42_001060"
     CDS             11363..11596
                     /locus_tag="RJE42_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDV5876492.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            11707..12972
                     /gene="murA"
                     /locus_tag="RJE42_001061"
     CDS             11707..12972
                     /gene="murA"
                     /locus_tag="RJE42_001061"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV5876493.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LEYQEDGIRVRAEGDLQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            13006..13446
                     /gene="fabZ"
                     /locus_tag="RJE42_001062"
     CDS             13006..13446
                     /gene="fabZ"
                     /locus_tag="RJE42_001062"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV5876494.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(13503..13943)
                     /locus_tag="RJE42_001063"
     CDS             complement(13503..13943)
                     /locus_tag="RJE42_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDV5876495.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            14137..14532
                     /locus_tag="RJE42_001064"
     CDS             14137..14532
                     /locus_tag="RJE42_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV5876496.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            14921..15616
                     /gene="sceD"
                     /locus_tag="RJE42_001065"
     CDS             14921..15616
                     /gene="sceD"
                     /locus_tag="RJE42_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDV5876497.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSADQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNDHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      15821..15923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16003..16692
                     /gene="tenA"
                     /locus_tag="RJE42_001066"
     CDS             16003..16692
                     /gene="tenA"
                     /locus_tag="RJE42_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDV5876498.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            16685..17515
                     /gene="thiD"
                     /locus_tag="RJE42_001067"
     CDS             16685..17515
                     /gene="thiD"
                     /locus_tag="RJE42_001067"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV5876499.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQNIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIVKGGHSNDTDIAKDYLFTKEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFDAVQKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            17499..18290
                     /gene="thiM"
                     /locus_tag="RJE42_001068"
     CDS             17499..18290
                     /gene="thiM"
                     /locus_tag="RJE42_001068"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV5876500.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
                     GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            18292..18933
                     /locus_tag="RJE42_001069"
     CDS             18292..18933
                     /locus_tag="RJE42_001069"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV5876501.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLGEYAKSDLTHVDYIGVGPIYPTPSKNDAHTPVGPEMIATFKEM
                     NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            19019..19891
                     /gene="yidC"
                     /locus_tag="RJE42_001070"
     CDS             19019..19891
                     /gene="yidC"
                     /locus_tag="RJE42_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV5876502.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGNNK
                     KGKNTQVVSKKKKK"
     gene            20221..21221
                     /locus_tag="RJE42_001071"
                     /pseudo
     CDS             20221..21221
                     /locus_tag="RJE42_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         7459 a         3196 c         4118 g         6448 t
ORIGIN      
        1 cactttgcta tattcctttt ttatttttag gaagggtggg cttttatgaa agatttgaca
       61 atgttattag atgaattaaa agatatgtcc ttttttaata aaggagatat atgtttaatt
      121 ggatgttcaa catcggaagt cattggcgaa aaaataggga ctgtcggttc gatggaagtt
      181 gcggaaacta tttttaatgc attagatgtt gtgagtaaag agacaggcgt tacttttgct
      241 tttcaaggat gtgaacatat caacagagcg ataacaatag aaaaatctca gtttaatcca
      301 ttaacaatgg aagaagtttc cgtcgttcca gatgtacatg ctggagggag tttggcaaca
      361 tacgcttttc agcatatgaa ggatccgatc gtggtcgaac atattactgt tccttgtgga
      421 attgatattg gtcaaacatt aataggcatg catattaaac atgtatgtgt acctgtaagg
      481 acctcagtaa agcaagtagg acaggcaatt gtaactattg ccacatctag gccgaaaaag
      541 attggtggcg aacgtgctaa ataccaataa gtttttttga aagaagggga gattttatgt
      601 cttatatcac caagcaagat aaagttatcg cagaagcaat cgagagagaa tttcagagac
      661 aaaatagcaa catagagtta atcgcatcgg aaaattttgt atcggaagcg gttatggaag
      721 cacaaggttc agtgttgact aataagtatg ctgaaggcta tccaggacgc cgatattatg
      781 gtggctgtga gtttgtagat gtaactgaaa gcatcgcaat tgatcgtgct aaagcattgt
      841 ttggagctga acatgtcaat gttcaaccac attcaggttc acaagcgaat atggctgttt
      901 acttagttgc attagaaatg ggcgacacag ttttaggtat gaatttgagt catggtggtc
      961 acttgacaca tggagcgcct gttaatttta gtggtaaatt ctacaatttc gttgaatatg
     1021 gagtagataa agacacagaa cgaatcaatt atgatgaagt tcgtaaatta gcgttagagc
     1081 ataagcctaa gcttattgtg gcaggagcat cagcatattc aagaacaatt gatttcaaaa
     1141 agtttaaaga aatcgcagat gaagtaaacg ctaagttaat ggtagacatg gcacatattg
     1201 caggattagt agcggcaggt ttacatccaa atccagtaga atatgctgat tttgtaacaa
     1261 ctacaacaca caaaacatta cgcggaccac gtggtggtat gattttatgt aaggaagaat
     1321 ataaaaaaga catagataaa acaattttcc ctggtattca aggtggacct cttgagcatg
     1381 ttattgcagc aaaagcagtt gcttttggag aagcgttaga aaataatttc aaaatgtatc
     1441 aacaacaagt ggttaaaaac gcaaaagttc ttgcagaaac attaattaat gaaggattta
     1501 gaattgtttc tggcggtaca gataatcact tagtagctgt tgatgtaaaa gggtctatag
     1561 gacttactgg taaagaagct gaagagactt tagattcagt tggtatcaca tgtaacaaaa
     1621 ataccattcc gttcgatcaa gaaaaacctt ttgtaacgag tggtatacgt ttaggtacac
     1681 ctgctgcaac aacgcgtgga tttgatgaaa aagcttttga ggaagttgca aaaatcatca
     1741 gtttagcatt gaaaaatagt aaagatgaag aaaaattaca acaagctaaa gaacgcgttg
     1801 cgaaattaac agctgaatat cctctatatc aataaacata cacaaagata ggagtaattt
     1861 ttatgagtaa agtacacgtt ttcgatcatc cattaattca acacaaacta agctatatta
     1921 gagacgtaaa tacaggtact aaagaattta gagaacttgt agacgaagtt ggtatgttga
     1981 tggcatatga agtaactaga gacttagagc ttcaggatgt tgatattgaa acgcctgtaa
     2041 ctaaaatgac ggctaaacga ttagctggta aaaaattagc tatcgttcca attttaagag
     2101 ctggtttagg tatgactgat ggtattttaa gtttagtacc tgcagcaaga gttggacata
     2161 tcggtttgta tcgagatccc gaaacattaa aggctgtaga gtattttgct aagttacctc
     2221 aagatattac tgaaagacaa atcattgttg ttgatcctat gttagcaaca ggcgcatcag
     2281 caattgaagc tattacttca ttgaagaaac gtggcgctaa aaatattcgc ttcatgtgtt
     2341 taattgcagc accagaaggc gtagaaaaga tgcatgaagc acatccagat gtagatattt
     2401 atattgctgc acttgatgaa aagctaaatg acaaagcata tatcacacca gggttaggcg
     2461 acgctggaga tagattgttt ggtactaaat agaagaatcg gagataagta gtttgatgaa
     2521 aaagattatg accatatttg gaacaagacc cgaagccata aagatggctc ctttagttaa
     2581 agctttggaa caagaaaaaa tgctcgagcc aattgttgtt gttacagcac agcatagaga
     2641 aatgcttgat tcagtattaa gtacctttga aatcaaacca aaatacgatt tgaatattat
     2701 gaaatcagga cagacacttt ctgagataac ttctaaatca ataactcaat tagaacaagt
     2761 gattcaatta gaaaaaccag acatggtact tgtgcatggt gatacgatga cgacatttgc
     2821 aggtggttta gcggcatttt ataaccaagt gccgattgga cacgttgaag cagggcttag
     2881 aagttatgat aaatattcac cttttccgga agaggtgaat agacaattag ttggtgtatt
     2941 agctgactta cattttgcac ctactaaaaa tgctgcatcg catttgttaa atgaagggaa
     3001 atattcagaa agtgttgttg tcactggtaa tacagcgatt gatgctatga aatatacagt
     3061 tgatgacaat tataaatcta acatcatgga taaataccat gacaaaaagt tcattcttat
     3121 gacagcacac cgtcgtgaaa atttaggtca acctatggaa aatatattta aagcagttag
     3181 acgcttgata gatgagtaca ccgatttagc gttagtatat ccaatgcata aaaatccaaa
     3241 agtccgagat gtggctcata agattttagg ggggcatgac agaattgaat tgattgaacc
     3301 tttggacgta gtagatttcc ataattttgc taaaaaatct tattttattt tgacagattc
     3361 aggtggaatt caggaagaag cgccatcatt taataagcca gttttagttt tgcgaagcgt
     3421 tacagaacgt cctgaaggtg ttgaagctgg cacattaaaa gtcattggaa caaataagca
     3481 aaatgtgtat caagctgcaa aagaattaat cgatgatgag agattatatc atcaaatgtc
     3541 tgaagcatct aatccttacg gtgatggatt tgcttcagaa agaatagtta atcatattaa
     3601 gtattatttg aatttaatca ctgaaaaacc aagcgatttc tgagaaaaat agactttttg
     3661 tctaaatgtg tctaaattgt gtctgaacat gcttattttt ttacaacaat ttgaatagaa
     3721 ttgacaccac aaatcgccta tattatcatt tagtttgtat gagtggaacg gttttcatga
     3781 ttgatatcag tcacacggag gcatataaaa tgagtcgttt ctacaacatt tttaaacagt
     3841 tcattcaata ttatttgtat ttattaataa tattgggagg attatacctt tttacacacc
     3901 atgcatttat cttaggatta atcattggtg ttactggttc tgcaatcaat actgttatat
     3961 ttgagagcta cttagccaaa gctaaaagac cagacactat gcatatttca actggaaata
     4021 tgtggcgata cttagttgca attattgctt gtatgatttg gtactttaat aaatcgcatg
     4081 taagtataat cggtataatt attggtttaa tgatttcata tgttgtagtt atcatacgtc
     4141 ctttactaaa ggtgagcaaa taaattaaga aagaggtgag attatggatc acaaatcccc
     4201 gctcgtgagt tggaatttat tcggttttga tatcgttttc aatttatcaa gtattttgat
     4261 gatacttgtt acggcgtttc ttgtttttct acttgctatc atttgtacgc gtaatttgaa
     4321 aaaaagacca actggcaaac aaaatttcgt tgaatggatt tttgatttcg tgaggggaat
     4381 cattgaaggt aacatggctt ggaaaaaagg tggtcaattc cacttcttag cagtaacgct
     4441 gattctgtac atttttatag ctaatatgtt aggtcttccg ttttctatag taacgaaaga
     4501 tcacacattg tggtggaaat caccaacagc tgatgcaaca gtgactttaa cgttgtctac
     4561 aacgataata ctgttaactc acttttatgg aattaaaatg cgtggtacga aacaatatct
     4621 taaaggttat gtacagccgt tttggccatt ggcaattatt aatgtttttg aagagttcac
     4681 ttcaacatta acgcttggtc tgcgtttgta cggtaacata tttgcaggtg agatactatt
     4741 aacattactt gctggcttat tctttaacga accagcatgg ggttggatta ttagtatccc
     4801 aggattaatt gtttggcaag cattttcaat atttgtagga acaatccaag catatatctt
     4861 tattatgctt tcgatggttt atatgtcaca taaagtggca gatgaacact aaaaatttca
     4921 ataattatat acaatcatag gaggaaatta aattatgaat ttaatcgcag cagcaatcgc
     4981 aattggttta tcagcattag gagcaggtat cggtaacggt ttaatcgttt caagaacagt
     5041 tgaaggtgta gcacgtcaac cagaagcacg tggtcaatta atgggtatca tgttcattgg
     5101 tgtaggttta gttgaggcat tacctatcat cggtgtagta attgcattca tgacatttgc
     5161 tggataatta acagataaaa gaggtcggga caaagcgcat aggacataat tcatgatgcc
     5221 atatatagta atatctttga actttattaa atagttgaga tatgaacgca ccatgcctat
     5281 cgcataaatt cagtaggtcc taacctcgtc gttttttcta tataacacta gcgattattt
     5341 taatgaaagg agtgtcatga acccgtgact gaaacagcta acttattcgt tcttggtgca
     5401 gctggaggcg ttgagtgggg tactgtgatt gtacaggtcc taactttcat cgtgttactt
     5461 gcgttactta aaaagttcgc atggggtcca ttgaaagatg taatggataa acgtgaaaga
     5521 gatattaaca gagatatcga tgacgcagaa caagctaagt taaatgcaca gaaacttgaa
     5581 gaagaaaata aacaaaaact taaagaaaca caagaagaag ttcaaaagat tttagaagat
     5641 gctaaggttc aagcacgtca acagcaagaa caaattattc atgaagcaaa cgtacgtgca
     5701 aacggtatga ttgaaacagc acaaagtgaa atcaatagcc aaaaagaacg tgccattgca
     5761 gatattaata atcaagtatc tgaactatca gtgttaattg cttctaaagt tcttagaaaa
     5821 gaaatttctg aacaagacca aaaagcattg gttgacaagt atctaaaaga ggcaggcgat
     5881 aaataatggt aaaagtagct aacaagtatg ctaaagcatt atttgacgtg tcattagata
     5941 caaacaattt agaaactatt aatgaagaat taacagttat aaatgaagca gtaaaagata
     6001 aaattgagca attgaaaatg gttgacagta atccaactca aacagcagaa caacgtcgtg
     6061 agttgattaa tggtgtattt actgacatta atccatacat taaaaatatg atgtacgtgt
     6121 tagcagataa tagacatatc tcattaatag ctgacgtatt caaggcgttc caaagcttat
     6181 ataacggaca ctacaatcaa gattttgcaa caattgagtc aacatatgaa ttgagtcaag
     6241 aagagttaga taagattgtc aaactagtaa ctcaacaaac gaagttatct aaagttattg
     6301 tagatacaaa aattaatcca gatttaattg gtggatttag agttaaagtc ggcacaactg
     6361 tattagatgg tagtgttaga aatgatcttg tccaattaca aagaaaattt agaagagtta
     6421 attaattata aagaggagtg acatagatgg ccataaaagc tgaagaaatc agtgcattac
     6481 ttcgctcaca aattgaaaat tatgagtcag aaatgtccgt aactgatgta ggtactgtat
     6541 tacaaattgg tgatggtatt gcattaattc acggattaaa tgacgttatg gctggtgagc
     6601 tagtagaatt ccataacggc gtacttggtt tagcccaaaa ccttgaagag tcaaacgtgg
     6661 gtgtggttat tttaggacca tacacaggta ttactgaagg tgacgaagtt aaacgtactg
     6721 gtcgtatcat ggaagtacca gtaggtgaag aactaatcgg aagagttgtt aatccattag
     6781 gacaacctat tgatggacaa ggaccgatta acacaactaa aacacgtcca gtagagaaaa
     6841 aagctactgg tgtaatggat cgtaaatcag tagatgagcc attacaaaca ggtatcaaag
     6901 caattgatgc tttagtacca attggtagag gtcaacgtga gttgatcatc ggtgaccgtc
     6961 aaacaggtaa aacaacaatt gcaattgaca caattttgaa ccaaaaagat caaggtacaa
     7021 tttgtatcta tgttgctatt ggtcaaaaag attcaacagt aagagcaaat gttgaaaagt
     7081 taagacaagc aggcgcttta gactacacta ttgttgtagc agcatcagct tctgaacctt
     7141 ctccattatt atatattgca ccatattcag gtgtaacaat gggtgaagaa ttcatgttta
     7201 acggtaaaca tgttttaatc gtttatgatg atttaactaa acaagcagca gcttatcgtg
     7261 agttatcatt attattacgt agacctccag gtcgtgaagc atacccaggt gacgtattct
     7321 acttacatag tagattatta gaaagagcag caaaattaaa cgatgactta ggtggcggtt
     7381 caattactgc attaccaatt atcgaaacac aagctggtga tatttcagct tatgtaccaa
     7441 caaacgttat ttcaattaca gatggacaaa tcttcttaca atctgattta ttcttctcag
     7501 gtgtaagacc agcgattaat gccggacaat ctgtatctcg tgttggtgga tctgcacaaa
     7561 ttaaagcaat gaagaaagtt gctggtacgt tacgtcttga cttagcgtca tacagagaac
     7621 ttgaatcatt tgcacaattc ggttcagacc ttgatgaatt tactgcaagt aaattagaac
     7681 gtggtaaacg tactgttgaa gtcttaaaac aagatcaaaa caaaccatta ccagttgaac
     7741 accaagtgtt gattatttat gcattaacaa aaggatattt agatgatatt ccagttgtag
     7801 atatcacacg ttttgaagac gagttaaacc actgggcaga atcaaatgct actgaactgt
     7861 taaatgaaat cagagaaact ggtggcttac cagatgctga gaagtttgac acagcaatta
     7921 acgaattcaa aaaaagcttt agcaaatctg aataataaac aagtttagta taaggtggtg
     7981 agatagtggc ttctcttaaa gaaatagata ctcgaataaa atcaaccaaa aaaatgaagc
     8041 agattacgaa agcgatgaac atggtatcaa gttcaaaact tcgtagagct gaaaaaaata
     8101 caaaacaatt cacaccatat atggataaaa tgcaagatgc aattactgca gttgcagggg
     8161 caagtagcaa tacaaaccat ccaatgctaa gacctcgtaa aattactaga agtggatatt
     8221 tagttatcac gagtgataaa ggtttagcag gtgcatatag tgcaaacgtg cttaaaaaat
     8281 tgattactga tattgaagcg aaacatcaag atagtagcga atacagtatt gtagttttag
     8341 ggcaacaagg tgttgatttc ctaaaaaata gaggttatga cattgagtat tctcaagtag
     8401 acgtacctga tcaaccttct ttcaaatctg ttcaagcact agcaaaccat gctatagact
     8461 tatacagtga agaagaaatt gatgaattaa atatatacta tagtcattat gtcagcgttc
     8521 ttgaaaacaa gcctacatct agacaagtat taccattatc tcaagaggat tctagtaagg
     8581 gacatggtca tttgtcttct tatgaatttg agccagataa agaatctatc ttaagtgtaa
     8641 tcttgcctca atatgttgag agtttgattt acggaacaat attagacgca aaagcaagtg
     8701 agcatgcaac acgtatgact gcgatgaaaa atgccactga taatgcaact gaacttattg
     8761 atgacttatc attagaatat aacagagcga gacaagcaga aattacgcaa caaattactg
     8821 aaattgttgg tggttccgca gcgcttgaat aatatttaaa ggaggaaaat agcatgggaa
     8881 ttggccgtgt aactcaagtt atgggtcctg taattgatgt tcgatttgaa cataacgaag
     8941 ttcctaaaat taataacgcc ttggttattg atgtgcctaa agaagaaggt acattacaac
     9001 taacattaga agttgcgctg caattaggtg acgacgttgt tcgtacaatt gcgatggatt
     9061 caactgatgg tgtccaaaga ggcatggatg taaaagatac aggcaaagaa attagtgtac
     9121 ctgttggtga cgaaacatta ggtcgtgtat ttaatgtact aggtgaaaca attgacctta
     9181 aagaagaaat tagtgattct gttcgccgcg atcctatcca tcgtcaagca ccagcattcg
     9241 atgaactttc aacagaagtt caaattttag aaacaggtat taaagtagta gatttactag
     9301 caccttatat taaaggtggt aaaatcggat tgttcggtgg tgccggtgta ggtaaaacag
     9361 tattaatcca agaattaatt aacaacatcg ctcaagagca cggtggtatt tctgtattcg
     9421 ccggtgtagg tgaacgtact cgtgaaggta acgatttata cttcgaaatg agtgacagtg
     9481 gtgtaattaa gaaaacagcc atggtattcg ggcaaatgaa tgagccacct ggtgcacgta
     9541 tgcgtgttgc attatctggt ttaacaatgg ctgaatattt ccgtgacgaa caaggtcaag
     9601 acgtattatt attcatcgat aacattttca gatttacaca agctggttct gaggtatctg
     9661 cattattagg tcgtatgcct tctgcagtag gttaccaacc aacacttgct actgaaatgg
     9721 gacaattaca agaacgtatt acgtctacaa caaaaggatc agttacttct attcaagcgg
     9781 tattcgtacc tgccgatgac tatactgacc cagcgccagc gacagcgttt gcccatttag
     9841 atgcaactac aaacttagaa cgtaaattaa ctgaaatggg tatttatcca gccgtggatc
     9901 cattagcgtc tacatcaaga gcattggaac catcaattgt aggtcaagaa cattatgaag
     9961 tagctcgtga tgtacaatca acacttcaaa aataccgtga attacaagat atcattgcta
    10021 tcttaggtat ggacgaatta tctgatgaag ataaacaaac agttgaacgc gcacgtagaa
    10081 ttcaattctt cttatctcaa aacttccacg tagcggaaca atttactggt caaaaaggtt
    10141 cttatgtacc tgttaagaca acagttgcaa actttaaaga tatcttagat ggtaaatatg
    10201 accatattcc agaagatgca ttccgtttag ttggtagcat ggatgatgtt attgcaaaag
    10261 ctaaagatat gggtgttgaa gtataacaat taggaggaat ggataatgaa tacattaaac
    10321 ctagatattg tcactcctaa tggttctgtt tacaatcgtg ataatgttga actcgttgtt
    10381 atgcaaacaa cagctggtga gataggtgtc atgagtggac atattccaac tgtagctgct
    10441 ttaaaaacag gctttgtaaa agtgaaattt cacgatggaa ctgaatatat tgctgtaagc
    10501 gatggctttg ttgaagttag aaaagataaa gtttcaatca ttgttcagac tgcagaaact
    10561 gcaagagaaa ttgatgttga aagagctaaa ttagccaaag caagagcaga gtctcacttg
    10621 gaaaatgatg acgacaatac tgatattcat agagccgaaa gagctttaga gagagcaaat
    10681 aaccgtttgc gtgtggctga attaaaatag taaataaagg gtcgaagatg tgatttcata
    10741 tcttcgaccc ttttttgaat tatattaatt taaagataca aaacatgaga gtggggaagg
    10801 aattgataaa gaaccattaa agatttatga tgtagtggtt ctttatcatt aaacacagct
    10861 aatgtgtatt taaaaatagg agggagtggg acagaaatga tattttcaca aaatttattt
    10921 cgttgttcac cccaacttgc acattaatgt aagctgactt tccgccagct tttgtgttgg
    10981 ggccccgccc ccaacttgca ttgtctgtag gaattggtgt tccaatttct ttatgttggg
    11041 gccccgccaa ctactgccaa atataacact atagagtcta gacattgatt tatgtctgac
    11101 tctcaagaat agttttactt tttacaatca ctaatagatt gctaaaatca aaatttcctt
    11161 tcaccactat ctacagtcga cgtttctttt ttgaaagtag ctacattttt ccatgccaag
    11221 atactttata gttatgatat ttatgtaaaa agaattatat agtaagttag cttaaacttt
    11281 actaaaaacg ggtattaaac tttgtatcat tatttaaatt tttcatgtac aatgtaatac
    11341 agtaatctta tgaggtgata aaatggatta tatcggacaa tatgcagtta tccatttagt
    11401 gttacatgtt gtatgtattt gtattgccta ttgggcttta caatcaatta gattagatca
    11461 attttttaaa aaaggatacg ccactcaatt acaagtgtgt atgatatttg ttgctatttt
    11521 attaggcact gcagtaagca attttattgt agatttgtta caatactcga cgcaggtaaa
    11581 atatttaata aaataagtct aactctatga tttgtaatca aaactagata taattaaata
    11641 atgacttaaa ataattttaa aatagggaaa tgtaaagtaa taggagttct aagtggagga
    11701 tttacgatgg ataaaatagt aatcaaaggt ggaaataaat taacgggtga agttaaagta
    11761 gaaggtgcta aaaatgcagt attacctata ttaacagcat ctttattagc ttctgataaa
    11821 ccgagcaaat tagttaatgt tccagcttta agtgatgtag aaacaataaa taatgtatta
    11881 acaaccttaa acgctgacgt tacatacaaa aaggacgaaa atgctgttgt cgttgatgca
    11941 acaaagactc taaatgaaga ggcaccatat gaatatgtta gtaaaatgcg tgcaagtatt
    12001 ttagttatgg gacctctatt agcaagacta ggacatgcta ttgttgcatt gcctggtggt
    12061 tgtgcaattg gaagtagacc gattgagcaa cacattaaag gttttgaagc tttaggcgca
    12121 gaaattcatc ttgaaaatgg taatatttat gctaatgcta aagatggatt aaaaggtaca
    12181 tcaattcatt tagattttcc aagtgtagga gcaacacaaa atattattat ggcagcatca
    12241 ttagctaagg gtaagacttt aattgaaaat gcagctaaag aacctgaaat tgtcgattta
    12301 gcaaactaca ttaatgaaat gggcggtaga attactggtg ctggtacaga cacaattaca
    12361 atcaatggtg tagaatcatt acatggtgta gaacatgcta tcattccaga tagaattgaa
    12421 gcaggcacat tactgatcgc tggtgccatt actcgtggcg atatttttgt acgtggtgca
    12481 atcaaagaac atatggctag tttagtgtat aaattagaag aaatgggcgt tgaattggaa
    12541 tatcaagaag atggtattcg tgtacgtgct gaaggagatt tacagcctgt tgacatcaaa
    12601 acattaccgc atcctggttt tccaaccgat atgcagtcac aaatgatggc attattatta
    12661 acagcaaacg gacataaagt agtaactgaa actgtatttg aaaatcgttt tatgcatgtt
    12721 gcagagttca aacgtatgaa tgctaatatc aatgtagaag gtcgtagtgc taaacttgaa
    12781 ggtaaaagtc aattgcaagg tgcacaagtt aaagcgactg atttaagagc agcagccgcc
    12841 ttaattttag ctggattagt tgctgatggt aaaacaagcg ttactgaatt aacgcaccta
    12901 gatagaggct atgttgactt acacggtaaa ttgaagcaat taggtgcaga cattgaacgt
    12961 attaacgatt aattcagtaa attaatataa tggaggattt caaccatgga aacaattttt
    13021 gattataacc aaattaaaca aattatacct cacagacagc catttttatt aattgataaa
    13081 gtagttgaat atgaagaagg tcaacgttgt gtggctatta aacaagtatc aggaaacgaa
    13141 ccattttttc aagggcattt tcccgagtat gcggtaatgc caggcgtatt aattactgaa
    13201 gcgttagctc aaacaggtgc ggtagctatt ttaaatagtg aagaaaataa aggtaaaatc
    13261 gctttatttg ctggtattga taaatgtcgt tttaaacgtc aagtagtacc tggtgatact
    13321 ttaacgttgg aagtagaaat cactaaaatt aaaggaccaa tcggtaaagg taatgctaaa
    13381 gctactgtcg atggtcaact tgcttgtagt tgtgaactta catttgcaat tcaagatgta
    13441 aaataaaaca aaaaacattc aaagatttaa tgtgttggca taatctttga atgtttttta
    13501 ttttactctt ctaatttttc atcctttaac tttggtttag actgcatcat tcgattaaat
    13561 gattttttta attcttcacc agataatcca tcatcaataa gttgttctaa taaactttca
    13621 gcatactgtt gacgtaaagt gtaaatatga cattcaaata cgatagacat tgttgtttta
    13681 aaatttttaa tacttttcac agtagattca aataaagctg gatcattggc tggacgcgtg
    13741 atgacgacac gaatatccaa taactgttca ttgtcttgat attccttaaa tgtttcataa
    13801 tgctcattag aaatgacaat ttcaagcagc caacccgtgc cactttcctc tttattaatt
    13861 attacaccat cttctaattc atattctata attctgccgt gttcattcac aatttgaaac
    13921 cttactgctt taaaagtttt catgttccca catcccatta gtttaatatt tatgattttt
    13981 gaattattta gaagatttaa ggtcatttca tgactaattt ctttaaatat aacaaataac
    14041 gagatgctat agctattaat tatataaaat ttgccatttt gaccattttt aagctagtaa
    14101 ttaagatata ggcataggag gtgaatcaca cactacatgc taaataaaat cgtaattgtc
    14161 gggagactga cgaaagacgc acaaatattt gaaaaggagg atagaaaaat tgcaacgttt
    14221 tgtgttgcaa cgcaccgaaa ttataaagat gaaaatggag aaatcgtctg tgattactta
    14281 ttctgtaaag catttggcaa gttagcttct aatatagaaa aatacactaa tcaaggtaca
    14341 ttggttggta taactggtca aatgagatca agaaagtatg ataaagacgg acaaacacac
    14401 tttgtcactg aattatatgt tgaaacaata aaatttatgt cccctaaatc ccaaaataat
    14461 gaaattctct cagatagtat tttagatatt gactctcaaa atatagataa tcatgactta
    14521 ttagaaattt aaatccaatt atcctaaaca tccttaatat acatttaatc catcctaaat
    14581 tttttataga tctaattaac ttgaaatata ctcacttaaa aacgacttac atgactagta
    14641 ctgttgtgta ggtcgtcttt ttgtgtagtt tttctaatag aaaatagaca tagattgcaa
    14701 aacgattaca taaaaaattg caaatgaaaa acatttttac tgtcatgaca attctcgata
    14761 gaatcatatc tgaaattatg ggggatttcg ttgtaatcac tgtgtaaaaa acaaggctat
    14821 ttttttattt tgttgtaacc tgcatggatt ttacagatgt tacagtagtc tctgtaaaac
    14881 ttttagagaa acaaattact tgtaggagga ttttaagatt atgaagaaaa cattactcgc
    14941 atcatcatta gcagtaggtt taggaatcgt agcaggaaat gcaggtcacg aagcccatgc
    15001 aagtgaagcg gacttaaata aagcatcttt agcgcaaatg gcgcaatcaa atgatcaaac
    15061 attaaatcaa aaaccaattg aagctggcgc ttataattat acatttgact atgaagggtt
    15121 tacttatcac tttgaatcag atggtacaca ctttgcttgg aattaccatg caacaggtgc
    15181 taatggagca gacatgagtg cacaagcacc tgcaactaat aatgttgcac catcagctga
    15241 tcaagctaat caagtacaat cacaagaagt tgaagcacca caaaatgctc aaactcagca
    15301 accacaagca tcaacatcaa acaattcaca agttactgca acaccaactg aatcaaaagc
    15361 atcagaaggt tcatcagtaa atgtgaatga tcatctaaaa caaattgctc aacgtgaatc
    15421 aggtggcaat attcatgctg taaatccaac atcaggtgca gctggtaagt atcaattctt
    15481 acaatcaact tgggattcag tagcacctgc taaatacaaa ggtgtatcac cagcaaatgc
    15541 tcctgaaagt gttcaagatg ccgcagcagt aaaattatat aacactggtg gcgctggaca
    15601 ttgggttact gcataagcca tttatgcata actaaccaat agttatataa gtaactttta
    15661 gattggaata tatcggggaa ttctcgatat gttccggtct tttttatttc gcaaatatgt
    15721 ataattatag aattattatt taaagataat aatacaactg agcaacaata attgaaaatg
    15781 ttgaaaccgt ttgctaagat taatttgcaa ttgaatacta aggaactact aggggagcct
    15841 aataatatgg ctgagatgaa ctgttcagac ccttatgacc tgatttggtt agtaccaacg
    15901 taggaaagta gttattgaag tgatggggct agtatagcaa cattgccatc agactacttt
    15961 catgttggaa gtagtttttt tatttaggga ggatttttta caatggaatt ttcacaaaaa
    16021 ttgtaccaag ctgcaaagcc aatcattaat gatatttatg aggatgactt tattcaaaaa
    16081 atgttattag gcaatataca agctgatgca ttacgtcatt atttacaagc tgatgcagct
    16141 tatttaaaag aatttacaaa cttatatgct ttgttaattc caaaaatgaa tagcatgaat
    16201 gacgtaaaat ttttagtaga gcaaatagaa tttatggttg agggtgaagt acttgcgcat
    16261 gatattttag cgcaaattgt tggtgaatca tacgaagaaa tcattaaaac aaaagtttgg
    16321 cctccaagtg gcgaccatta cataaaacat atgtatttcc aagcgcatag tcgtgaaaat
    16381 gctatttata caattgctgc aatggcacct tgtccatata tttatgcaga attagctaag
    16441 cgctctcaaa gtgatcataa gcttaataga gaaaaagata cggcaaaatg gtttgatttt
    16501 tatagtactg aaatggatga cataattaac gtatttgagt cattaatgaa taaattagcc
    16561 gaatcaatgt ctgacaagga attggaacaa gtaaagcaag tatttttaga aagttgcata
    16621 catgagcgtc gatttttcaa tatggctatg acactagaac aatgggaatt tggaggaaaa
    16681 gtaaatgatt aaacctaaaa tagcattaac cattgcaggt actgatccaa caggtggtgc
    16741 cggcgtaatg gctgatttaa aatcatttca ttcatgtggt gtatatggta tgggcgtcgt
    16801 tacaagtatt gttgctcaaa atacattggg cgtacaaaat attcataatt taaatcatca
    16861 atgggtagat gaacaacttg atagtgtctt caatgatacc ttaccgcatg ctattaaaac
    16921 ggggatgatt gctacagcag atactatgga aacgattcgt cattatttaa tgcaacatga
    16981 atctattcca tatgtaattg atcctgttat gttggcgaaa agcggtgatt cattaatgga
    17041 taatgacaca aagcaaaact tgcagcatac gttattgcca ttagctgatg tagtaacacc
    17101 gaatttacca gaagctgaag aaataacggg actaaccatt gatagtgaag aaaaaattat
    17161 gcaggctggc cgcatcttta ttaatgaaat tggtagtaaa ggtgtcatcg ttaaaggcgg
    17221 tcattcaaat gatactgata tagcaaaaga ttatttattt actaaagaag gtgttcaaac
    17281 atttgaaaat gaacgattta aaacaaaaca tacgcatgga acagggtgta cattttcagc
    17341 agttataacg gcagaacttg caaaaggtag accattattt gatgctgtac agaaggctaa
    17401 aaagtttatt tcaatgagta tacaatatac gcctgaaatc ggtcgtggta gaggtccagt
    17461 gaatcatttt gcatatttaa agaaagaggg attagacgat gaattatcta aataaaatac
    17521 gtattgaaaa cccattaaca atttgttata caaacgatgt agttaaaaat tttacagcga
    17581 atggtttatt aagtattggt gcgagccctg caatgagtga agctcctgaa gaagctgaag
    17641 aattttacaa agttgcacaa gcgctattaa tcaatatcgg tactttaaca gcacaaaatg
    17701 aacaagatat tattgcgatt gctcaaacag caaatgaggc aggcttacct attgtatttg
    17761 accctgtagc tgttggtgct tctacatatc gaaagcaatt ttgtaaatta ttattgaaat
    17821 cagcgaaagt atcagtaatt aaaggcaatg catctgaaat attagcgttg attgatgata
    17881 cagcaactat gaaaggtaca gatagtgatg ctaatcttga tgcggttgca atagcgaaaa
    17941 aggcttacgc aatatataaa actgcaatag taatcacagg taaagaggac gttattgttc
    18001 aaggtgataa agccatcgta ttagctaatg gatctccatt attagcacga gtaactggag
    18061 ctggttgttt attaggaggc gttattgctg gatttttatt tagagaaaca gaaccagaca
    18121 tagaagcgtt aattgaagcg gtaagcgtat ttaatattgc tgctgaggta gctgctgaaa
    18181 atgaaaattg tggtggtcct ggtacgtttt caccattgtt gcttgatacg ttatatcatt
    18241 taaatgaaac aacctatcaa caacggattc gcattcaaga ggtggaatag tatgtttaac
    18301 caatcttatc taaatgtata ttttatttgt gggacctccg atgttccgag tcatcgaact
    18361 attcatgaag tattagaagc agcattaaag gctgggatta cacttttcca atttcgagaa
    18421 aagggtgaat cagctttaaa aggaaacgat aaacttgtat tagctaaaga attacagcat
    18481 ctctgtcatc aatatgatgt gccttttatc gttaatgatg atgtatcctt ggcaaaagaa
    18541 attaacgcgg atggtataca tgtcggtcaa gatgatgcga aagtcaaaga gatagcacaa
    18601 tatttcactg ataaaattat tggactaagt attagtgatt taggtgagta tgcaaaatct
    18661 gatttgactc atgttgatta tataggtgtt ggaccaatct atcctacgcc gtcgaaaaat
    18721 gacgcgcata caccagtagg tccagaaatg attgcaacat ttaaagaaat gaatccgcaa
    18781 ctaccgattg tagctattgg cggtataaac actagtaacg tggcaccgat tgtagaagca
    18841 ggtgccaatg gaatttcagt catttcagcc atttctaaaa gtgaaaatat tgaaaagact
    18901 gttaatcgat tcaaagattt ttttaataat tagttaaatt tttccactta ttgtttcata
    18961 cgtactcatt aatgtgatag aataaatcct atgtgaatat aatgatagag gtggaaaaat
    19021 gaagaaaaaa gcgttactac cattattttt aggtattatg gtctttttgg ctggttgtga
    19081 ctattctaaa cctgaaaaac gtagtgggtt tttctacaat acattcgtag atccaatgaa
    19141 aaatgtattg gattggttgg gaaataactt attaaacgac aattatggtt tagctattat
    19201 tatccttgta ttggtaattc gtattatttt attaccattc atgttgtcaa actataaaaa
    19261 tagtcatatg atgcgtcaaa aaatgaaagt tgcaaagcca gaagttgaaa aaattcaaga
    19321 aaaagtgaaa cgtgcgcgta cacaagaaga aaaaatggct gcaaaccaag aattaatgca
    19381 agtatataaa aagtatgaca tgaacccgat taagagtatg ttgggttgtt taccaatgct
    19441 aatccaattg ccaatcatca tgggattata ctttgtactt aaagaccaac ttgtagatgg
    19501 tttgtttaaa tatccacact tcttatggtt cgatttagga cgtcctgata tttggattac
    19561 aattattgcc ggtgttttat actttataca agcatatgta tcaagtaaaa cgatgccaga
    19621 cgaacaacgt caaatgggtt acatgatgat ggtcatttca ccaattatga ttatctggat
    19681 ttcattaagc tcagcatcag cacttggttt gtactggtca gtcagtgcgg cgttccttgt
    19741 agttcaaaca cactttgcga acatttatta tgaaaaagtc gctaaaaaag aagtacagcc
    19801 tttcattgaa gcgtatgaaa gagagcacaa cggcggcaac aataaaaaag gcaaaaacac
    19861 acaagtagtg tctaaaaaga aaaagaaata accttagcaa gtccaaataa tgtgccccct
    19921 aaaagttgaa gataatttta actttatagg gcgcacatta tttttcgctt caatagcgaa
    19981 acttagtgtc acattataca atatgtgata aatagaaaca gcagtaagat gatttcaaat
    20041 gtgaaagtca tcctactgct gtttttattt gaaataatat ggttctataa taaatcggac
    20101 tgatagaaaa agttttttac ttttcatctg tccgattttt tgattttgaa tataaaaaaa
    20161 gcgcaattat ctctataatt aaaagtgact aaacaaataa ctaaaggaga taatgcgcct
    20221 atgaataatg ttatactaaa aacctttgat tttaaagata aaaatattat ttttgacggt
    20281 aaacttgaga aaattgagta taaagggaga aagtgtttct tttattacgc aaaactaatc
    20341 tatacacctg aggtgtgccc aaactgcaat tgtaaaaaca ttaatggtaa tttaatcaaa
    20401 aatgggtcta agacttctag aatcactatg cctaaaatct ccgaatatcc aacttatatc
    20461 atgttgcgta agcaacgttt taaatgtaaa acgtgcgaac gatactttac agctgaaaca
    20521 cctgaagttg ataaatactg ttttatatca aaaaaacacg atcagctgta cttaataaag
    20581 cagcagaaat tcgttctgaa aaatctatag cgaaatcctg ctctgtgtca gctactacag
    20641 tttctcgtat cattgatgaa gccgctcaat cgcttcatca atcgccgtat tctgcatttc
    20701 ctgaacatat catgatggat gaatttaaaa gtgttaaaaa tgtttctggg aaaatgagtt
    20761 ttatatacgc tgatgcacaa acgcatcata ttattgatat tgttgaagat cgtagattaa
    20821 gtgagttgaa aaagtatttt tatcgttttt cacttaaagc tagaaaacgt gtgaaaacag
    20881 tttcgattga tatgcatgaa ggctacatga cattgattaa agagatgttt ccgaacgcta
    20941 aaatagtcat cgatcgattc catattgttc aattattaaa tcgtgcctta aatggtattc
    21001 gagtcgctgt tatgaatgaa ttaagaacga cgaatcaacc tttatataat aagtttaaaa
    21061 gatatgcgaa attactatta aaacctggag aagatttagg ggcatttgaa tatcgtaaag
    21121 tagctttatt taaggaatgg aagacacaaa agggtatcgt caagtattta ttagatcaag
    21181 atgactcgtt aaatgacgcc taccaatata tcaatcagct a
//
LOCUS       DAPIDM010000032        20826 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000032 DAPIDM010000000
VERSION     DAPIDM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.032"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..793
                     /gene="scpA"
                     /locus_tag="RJE42_001072"
     CDS             <1..793
                     /gene="scpA"
                     /locus_tag="RJE42_001072"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDV5876503.1"
                     /translation="SKYTINVSSFLSKALNQYKDQQITILTNSKGYYVVTQNHKAKLV
                     LKTPRLEDKKVKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFK
                     IRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQQFQFTGLTPREMI
                     YFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAILGSRVESRNGMHAGHAMAVVGNAK
                     LDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
     gene            824..1147
                     /locus_tag="RJE42_001073"
     CDS             824..1147
                     /locus_tag="RJE42_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDV5876504.1"
                     /translation="MEQFELFSIDKFKCNSEATYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHK"
     gene            complement(1440..1613)
                     /locus_tag="RJE42_001074"
     CDS             complement(1440..1613)
                     /locus_tag="RJE42_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDV5876505.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(1594..2196)
                     /locus_tag="RJE42_001075"
     CDS             complement(1594..2196)
                     /locus_tag="RJE42_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDV5876506.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2466..3287)
                     /gene="nadE"
                     /locus_tag="RJE42_001076"
     CDS             complement(2466..3287)
                     /gene="nadE"
                     /locus_tag="RJE42_001076"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV5876507.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALQFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKQLYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKSVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3280..4749)
                     /locus_tag="RJE42_001077"
     CDS             complement(3280..4749)
                     /locus_tag="RJE42_001077"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV5876508.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKAARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            4933..6009
                     /locus_tag="RJE42_001078"
     CDS             4933..6009
                     /locus_tag="RJE42_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDV5876509.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKNGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHDHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6029..6823
                     /locus_tag="RJE42_001079"
     CDS             6029..6823
                     /locus_tag="RJE42_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDV5876510.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DASIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGSNTISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMYDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            6895..7002
                     /locus_tag="RJE42_001080"
     CDS             6895..7002
                     /locus_tag="RJE42_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876511.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7013..8575)
                     /locus_tag="RJE42_001081"
     CDS             complement(7013..8575)
                     /locus_tag="RJE42_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV5876512.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSTGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(8780..9877)
                     /locus_tag="RJE42_001082"
     CDS             complement(8780..9877)
                     /locus_tag="RJE42_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDV5876513.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNHGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKNCSFEGFVDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDVIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10249..10809
                     /locus_tag="RJE42_001083"
     CDS             10249..10809
                     /locus_tag="RJE42_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDV5876514.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            10862..11791
                     /locus_tag="RJE42_001084"
     CDS             10862..11791
                     /locus_tag="RJE42_001084"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDV5876515.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(11984..12157)
                     /locus_tag="RJE42_001085"
     CDS             complement(11984..12157)
                     /locus_tag="RJE42_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876516.1"
                     /translation="MNVVAVNFKWSFELYILKVENRAMNCVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12209..13588
                     /locus_tag="RJE42_001086"
     CDS             12209..13588
                     /locus_tag="RJE42_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV5876517.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTNDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(13708..14736)
                     /locus_tag="RJE42_001087"
     CDS             complement(13708..14736)
                     /locus_tag="RJE42_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDV5876518.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTDTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15007..15144
                     /locus_tag="RJE42_001088"
     CDS             15007..15144
                     /locus_tag="RJE42_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876519.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFVICTNLIKNL
                     I"
     gene            complement(15196..15576)
                     /gene="blaI"
                     /locus_tag="RJE42_001089"
     CDS             complement(15196..15576)
                     /gene="blaI"
                     /locus_tag="RJE42_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDV5876520.1"
                     /translation="MANKQVEISMAEWDVMNIIWNKKSVSANEIVVEIQKNKEVSDKT
                     IRTLITRLYKKEIIKRYKYNNIYFYSSIIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(15566..17323)
                     /gene="blaR1"
                     /locus_tag="RJE42_001090"
     CDS             complement(15566..17323)
                     /gene="blaR1"
                     /locus_tag="RJE42_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDV5876521.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSFFKFNNLNNQEPTVESNSHNLNPNINTTKPVHEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLNSLLYLKYLKKQSLYLNEKEKDKINKILFNHQYKRNIV
                     IRKAESIHSPITFWYGKYIILIPSLYFKSINDKKLKYIILHEYAHAKNRDTLHLIIFH
                     IFSIAMSYNPLIQIVKRKMIHDNEVEADRFVLNNINKNEFKSYAEAIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKRRLINIKEGNLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHNHYPFDSWNKDQDLNTAIQNSVNWYFERISNQLS
                     KNYTSDQLKQLNYGNKNLGSYKAYWLEDSLKISNLEQVIVLKNMMEQNNHFSKNEKKQ
                     LSSSLLIRKNENYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFSTHLSDEKASGE
                     NAKFINEKILKEMGVLNGQ"
     gene            17430..18275
                     /gene="blaZ"
                     /locus_tag="RJE42_001091"
     CDS             17430..18275
                     /gene="blaZ"
                     /locus_tag="RJE42_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDV5876522.1"
                     /translation="MKKLILLIAIALVLSACNSTSSHAKELNNLEKKYNANIGVYALD
                     TKSGKEVKFNADKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMKYSDNTANNKIINEIGGIKKIKKRLKKLGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMLNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKNQSEPIILVIFTNKDNKSDKPNDKLISETAKN
                     VINKF"
     gene            18589..18999
                     /locus_tag="RJE42_001092"
     CDS             18589..18999
                     /locus_tag="RJE42_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDV5876523.1"
                     /translation="MIPNPNAPDEYKYETDYRKIPRKYLNPKIPQGRGKIKWQAFATL
                     PQQFEILEQIIKDQNKIEKPLLTHDSLDNLDQIFQIKIQNDELCTISYWEDGNISKYT
                     GKILKKDEISNTFSFSDTNNNIYNLNNANVCSIT"
     gene            complement(19042..20412)
                     /locus_tag="RJE42_001093"
     CDS             complement(19042..20412)
                     /locus_tag="RJE42_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876524.1"
                     /translation="MDNKIEKILEDINKLQGYKVTYQYATAINIKDNNMNPWFEKIEE
                     ILLNVKGVIRILSPDNPPVNYDYWILPGRYKRHTKEYHEKLEKFLEKFATNANINDGL
                     FDQNNIHGYKYKKYIFTPHIEITHENTLNLFFLTLTIFNNGTFVIEIIEDLESINFGS
                     DLYSTFSCVKDKIYPLINQEKNNRQRQYQLYGNRHCKNIRDVSRQINRIIKINSKDIF
                     EYKVSKNMGFEVYYLANENEFTDDPFYEDVKIQEWLVNAPIFSFEGKSKHFRQDGYSD
                     NYINFLNKASVFVVWMDEQEEDVLEKCSQMALYFNSSANFFFLETMLNELQLYGYQQY
                     NLNGKKEALYFRQWILNFKRSIAIIYRTELLPSLYLYLYLKNNNDFKSPQYIEELNKE
                     ELSLIELEQSYKEEERSEKLNKLLLIISLLSIFQVVEIFFSDKNNIFNISMILIMILL
                     EVFRKR"
     gene            complement(20417..20782)
                     /locus_tag="RJE42_001094"
     CDS             complement(20417..20782)
                     /locus_tag="RJE42_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5876525.1"
                     /translation="MEKFNRQEQLKQLHAERKVKTEKKVDKAINDLVQKNKEINFNIV
                     SKHSKVSKATLYKNNKIRKKIEKLREQNREIFVHKNKNDGKDALISSLKRKVNSLEKE
                     KKMLKEEISVLYSRLYEDI"
BASE COUNT         7176 a         3431 c         2848 g         7371 t
ORIGIN      
        1 ttctaaatac acaatcaatg tttcttcatt tttatctaaa gccttaaatc aatataaaga
       61 tcaacaaatt actattttga caaactcaaa aggttactat gtggttactc aaaatcataa
      121 agctaaacta gtactaaaaa caccacgtct agaagataaa aaggtaaaaa agaccgagag
      181 tatacctact ggcaataatg taacgcaact aaagcaaaaa gcttctgtca ctatgccaac
      241 tagtcaattt aaaagtaata attatactta caacgagcaa tatgtaaata aacttgagaa
      301 tttcaagata agagagactc aaggtaacaa tggttggtgt gcaggctata cgatgtctgc
      361 attactaaat gcaacttata atactaacaa atatcatgca gaagctgtga tgagattttt
      421 acacccaaac ttacaaggac aacaattcca atttactggt ttaacgccaa gagagatgat
      481 ttattttgga caaactcagg gaagaagtcc acaacttctc aatagaatga ctacttataa
      541 tgaagttgac aatcttacta aaaataataa aggaattgct attttaggta gtagagtaga
      601 atcaagaaat ggtatgcacg cagggcatgc catggcagtt gttgggaacg ctaaattgga
      661 taatggacaa gaggttataa ttatatggaa tccatgggat aatggtttta tgactcaaga
      721 tgctaaaaac aatgttattc cagtatccaa tggcgaccat tatcaatggt attcatctat
      781 ttatggttat taaaatttgt attttagaaa ggtaaataat attatggaac aatttgaatt
      841 atttagtatt gataaattca aatgtaattc agaggctacg tattatctta atattattga
      901 gggagaatgg catcctcaag atttaaatga tagcccttta aaatttattc tcagtacctc
      961 agacgattct gattacattt gcaaatatat aaatacagaa cacaaacaac tcacattata
     1021 taataaaaat aatagctcaa ttgttattga aatatttata ccaaatgata ataaaatact
     1081 actaacaatt atgaacacag aagctttagg aacttctcct agaatgactt tcattaagca
     1141 taaataataa gactatctag atttatttaa gtaaactatt aatgttatta aaggtctcta
     1201 ggaagaaaaa atttcatgcg atgagattgt tttagaaaaa gcgacgatat atataattta
     1261 acaatgattt taaaatagta agaaccccca gcatatgctt tatatagctt gctgggggtt
     1321 tttcatacaa tatggattga gcatcttgca tgtataaaca cataggctat tcaaagtaaa
     1381 attactcaaa tttgaataac ctttcctctt caaatagtct ttatttaaat tggattatgt
     1441 tattggattt tcttacctac aaaaatgatt tcttgatgtg atacttcaac cgtgccatct
     1501 ttttgctcct gaaacacggg tttttcaata cgttttaccg gcatataccc ttgcattttc
     1561 attcgtgaca aacaatcagc aattgtttcg ttctcattta ctttgaattt catgttctac
     1621 tacactttcc agttcttctg gttcatagtc tttaagcttt ctacgacgaa tgcctttagt
     1681 ccagaatcca ccatgtatgt ttcgaggttc atacgcaatg ataaatgctt tcggatctaa
     1741 attttttatc gtatccatca atttacgttc atattttctt ggtgttaaaa tttgcatcac
     1801 catacgacta ccatctctac caaatgcagc atagtgcgta acgccatatc ctaaatttcg
     1861 aagttcattc ggtaaatcta actcatattc tgctgaagtt acatttacaa ctgtataacc
     1921 taatgccagt ttttcttcta ttttcatacc aacaatgata cctattgaaa aaccaaatgc
     1981 gtatgcaata atattttgaa tatggtctaa attagacata accaaaccta aaccaacgat
     2041 ataaactaat acttctaaaa aactaactga tgcagcaata taacgataac ctttcaacgt
     2101 taaaattgtt cgcatcgtta aaaacgttac ataacaaacg ttaatgataa atatagttag
     2161 taccattaac catggatttt ctgttacgaa tgacataagg cgccccactc tctttcaatt
     2221 acactttaaa tatcaaactg cttcaaacta accttaataa atgcatatta atataatata
     2281 gtacgaaaac tctcataaat aaatagcgaa tttgtcataa ttaaaattta ttctcaaatg
     2341 aacatactta ctttatttca tacattatca atttatatac tgaagtagcc tagttgaatg
     2401 tggtttaacg ttattaattc taactcatat ttaatttaag gcacacgtta gaagaaaaaa
     2461 ttaaattagg attttggcca cgtgtatctt gtatatgcaa gttcacgttt gtgtgcattc
     2521 cgtatataat gattttcaat tactttttgt tcttctggcg taactgactt accttctaaa
     2581 taattatcaa tcgcctcata agttacacct aatgcatctt catctggaag ctgtggttta
     2641 tcatcttcta aatcagcagt tggtgttttt tcatacaatt gttttggtgc accaagataa
     2701 gctaataact gacgaccttg gcgcttattc aaaccgaata taggtgcaat atctgcagca
     2761 ccatcaccat atttcgtata aaaaccagtt atattttcag ctgaatgatc tgttcctact
     2821 acaatacctt gtcggtttga agcaattgaa aattgtactt tcatacgttc gcgagccttt
     2881 tcattacctt tttggaagtc tgttaggaca atcccagctt ctttcaatga ttgcacactt
     2941 tgatcaactg caggcttaat attgactgtt actatttcat ctggctcaat gaattgtaaa
     3001 gcttgctcaa cttcatcagc atctttttga actccataag gtaatttaac tgcaataaac
     3061 gtacaatcaa tgccttcttc acgtaattcg ttaacagaca tttgtactaa ttttccaact
     3121 aatgtagaat cttgtcctcc tgaaatacct aacactaaag attttataaa tgaatgtgat
     3181 tgtacataat tttttataaa ttgttttaat tccataattt cttcagcact atcgatacgc
     3241 tttttcactt tcatttcttg tacaataacg tcttgtaatt tactcattat cttcttccat
     3301 ctccttaacg tgttccgcaa cttcaaaaat acgtttatgt ttattatccc aacatgcctt
     3361 gcttaaatcg actggatatt cttgtggatt aaggaaacgc ttattttcat cccaaataga
     3421 ttgtaatcct tgtgctaaat attcacgtga ttcatcttct gttggcattt gatatactaa
     3481 tttaccattt tcataaatat tatgatgcaa atcaatggct tcaaaagatt ttataaattt
     3541 cattttataa gtatgcactg gatggaataa ttttaaaggc tgttcatcgt atggattttc
     3601 attttccaaa gtaatataat cgccttctgc cttacctgtt ttcttgttta taatgcgata
     3661 tacatttttc ttacctggcg tcgtaacctt ttcagcgtta tttgataatt taatacgatc
     3721 actatatgaa ccatcttcat tttcaatagc tacaagttta tatactgcac ctaatgctgg
     3781 ttgatcgtat cctgtaatca gctttgtacc aacgccccaa gaatctactt ttgcaccttg
     3841 tgctttcaaa ctcgtaatcg tttcttcatc caaatcatta gacgcgataa ttttagtttc
     3901 agtaaatcct gcttcatcaa gcatacgtct tgccgcttta gataaataag cgatatctcc
     3961 agaatccaat cgaataccta caaagttaat tttgtcacct aattcttttg caactttaat
     4021 tgcatttggc acgccagatt ttaaagtatg gaatgtatct actaggaaca cacaattttt
     4081 atgtctttca gcatattttt tgaaggcaac atattcatct ccataagttt ggacaaatgc
     4141 gtgtgcatgt gtaccagaca caggtatacc aaataattta cccgccctaa cattacttgt
     4201 agaatcaaag cccccgatgt aagcagctct agcgccccac aatgctgcat caatttcttg
     4261 cgcacgacgt gtaccaaact ccattaattt atcatttgat gcaatttgac gaattctact
     4321 agcctttgtt gtaattagtg tatggaaatt tacaatgttt aataaaattg tttctattaa
     4381 ttgcgcttga atcaatggtg cttctacgcg taacaatggt tcgttaccaa agcataattc
     4441 gccttcttgc atcgaacgga tgctgccagt gaattttaaa tcttttaaat atgataagaa
     4501 atcatccttg tagccaatag actttaaata ttccaaatca gattctgaaa atccaaaatg
     4561 ttctataaaa tcaatgacgc gttttaaacc attaaaaaca gcatagccac tattaaatgg
     4621 catttttcta aaatacaaat caaatacagc cattttttca tgaatattat cattccaata
     4681 actttcagcc atatttattt gatataagtc attatgtaac attaaactgt cgtcttctaa
     4741 ttggtacact cgtatctctc caatcgacct aaatattttc ttacatttta tcataattca
     4801 tttttttata tacataagag ccccttaatt tccatacttt taattaaaat caaccaacaa
     4861 tttaatgaca tatacataat ttttaagagt attttaataa tgtagactat aatataaagc
     4921 gaggtgttgt taatgttatt taaagaggct caagctttca tagaaaacat gtataaagag
     4981 tgtcattatg aaacgcaaat tatcaataaa cgtttacatg acattgaact agaaataaaa
     5041 gaaactggga catatacaca tacagaagaa gaacttattt atggtgctaa aatggcttgg
     5101 cgtaattcaa atcgttgcat tggtcgttta ttttgggatt cgttaaatgt cattgatgca
     5161 agagatgtta ctgacgaagc atcgttctta tcatcaatta cttatcatat tacacaggct
     5221 acaaatgaag gtaaattaaa gccgtatatt actatatatg ctccaaagaa tggacctaaa
     5281 attttcaaca atcaattaat tcgctatgct ggctatgaca attgtggtga tcctgctgaa
     5341 aaagaagtta cacgcttagc aaatcactta ggttggaaag gaaaaggtac taattttgac
     5401 gtgttaccac tgatttacca attacctaat gagtcagtta aattttacga atatcctacg
     5461 tcattaatta aagaagtacc tattgaacat gatcattatc caaaattaag aaaattgaac
     5521 ttaaaatggt atgcagtccc tatcatttcc aatatggact taaaaatcgg tggcattgtg
     5581 tatccaactg caccctttaa cggttggtat atggtaactg aaattggcgt acgtaacttt
     5641 attgatgatt accgttacaa tttactagaa aaagttgcag atgcgtttga atttgataca
     5701 cttaaaaata attcatttaa taaagatcga gcacttgttg aattgaacta tgctgtgtat
     5761 cattccttta aaaaagaagg cgtatcaatt gtcgatcatt tgaccgctgc aaagcaattc
     5821 gaactattcg aacgtaatga agcacaacaa ggtcgtcaag ttaccggaaa atggtcttgg
     5881 ctagcaccgc cattatctcc aacattgacg tcaaattatc atcacggata tgacaataca
     5941 gtaaaagatc caaacttttt ctataaaaag aaagaatcaa atgctaacca gtgccctttc
     6001 catcattaag ttagtagagg tgtagcatat gcaattatat tatttaggcc ctaaaggtac
     6061 attttcatat ttggcttgtc gtcagtattt ttctgaaaat gaagctactt ttcaaccaaa
     6121 gtcaaattta tttgaagtca tcaaggcagt tgcagatgat gacgcatcga ttggtgttgt
     6181 ccctattgaa aattctatag aaggaacaat caacattgtg gcagatgcac ttgcacagca
     6241 agatgtcttt gcacacggtg aaatacgttt agatattaac tttgcattat atggtaatgg
     6301 ttcgaacacc atttctgata ttaaaaaagt gtattctatt gcaccagcga tcagtcaaac
     6361 aacaaattac atacatcaac atcaatttga ctatgattat gtcgacagta caattcaaag
     6421 tttgactaaa attgaaaatg gcgttgcagc aattgcacct ttaggtagtg gtgaagctta
     6481 tggttttaca cctattgata cacatattga agactatcca cataacgtga cacgtttttt
     6541 agttataaaa aatcagcagc aatttgatca aaatgcgaca tcattgatgt ttttaattac
     6601 accaatgtat gataaacctg gattgttagc aagtgtgctt aatacatttg ctttgtttaa
     6661 tattaattta tcctggattg agtctcgtcc tttaaaaaca caattaggta tgtatcgatt
     6721 tttcgttcag gctgattctg caattacaac cgatataaag aaagtcattg ctattttaga
     6781 aaccttagat ttcaaagtag aaatgattgg tgcttttaat taaactttat tttctaggtt
     6841 ctacaattta aataaaataa agagtcaacc aaggattaaa ataggaatcc atccttggtt
     6901 gactttcttt tgtttgtatt gtttttacac gatgctgcgg tatacgtttc gttaccactc
     6961 gttcaagcaa ttgctaatgt aacattttat ttttcattct aatctgcaat tattatttca
     7021 atggcagtgg ttgatttata tcaataccta aaactatagg cattacataa tacacgacta
     7081 aaataataat tattgcactg attaagttta cccagaatcc tactgatgcc atttgtttga
     7141 tagatatttt acctgaacca aagataattg cattcggtgg tgtccctact ggtaacatgt
     7201 atgcacagtt agccgccata gctgctggtg ccataagtag taatggatgc actccaacag
     7261 caacagataa cgttgctaaa atcggtaaaa tcatcgttgc agttgcggta ttagatgtca
     7321 cttcagttaa aaataggaca aagatcgtta tgacgattac aataagaatc ggactgacac
     7381 catttaatga tttcaactgt tcgcctaacc attttgctaa accactttca gaaatacctt
     7441 tcgctaatgc taaaccgcca ccaaataaaa ttaatacacc ccaagggagc tcttttgcaa
     7501 cttcccagtc aatgatacgg cgatgttttt cagtattttt agctggaata ataaataata
     7561 atattgatat aaaaatagca atcgtaccat ctgcaacgga tgacgtaact tcccattttt
     7621 tcagaagaaa ctctcttgta atccataata agctggcaag tacaaagata gtttgtacta
     7681 ccttttcttc atacttcatt ttgccaagct cgtctaactt ttgtttaatt aacgtctgac
     7741 caccaggtaa atatttcaaa tcatgtctaa acgcaacata tcttaaatag agccaagtaa
     7801 tacctaacaa tacaatgacc gttggaatcc ctacaatcat ccatttagca aaactaattt
     7861 catgtccaaa atgttgcatg tattgtcctt ttaaaataat tagcggcggc gttccgataa
     7921 gtgtacctaa tccgccaatc gtacctgcat agccaattgc taaaactaga gatttttcaa
     7981 acttttgaat acttgtttga ttcgtattgg cttcttgtaa atcatgtgct tccttaataa
     8041 ttgctaaacc aatcggaatc atgatcatta cagctgcagt gttcgataca aacatagata
     8101 agaatcctgt tgccaccatg aatccaagta atatttttga agtgcttgca ccaattaaat
     8161 taataattgt caacgctact ctcgtatgta aattccatct ttccattgca attgccaaaa
     8221 taaatccacc taaaaacaaa aagataatat cattgccata ttcggatgat acttgttctg
     8281 gtgtaagtat gtgacctaat ggtaataaca caattggtaa taagctcgtt gctgcaatag
     8341 gaattgcttc agtaatccac caagtcgcaa tccataaagt aatcgctaag acatagacgc
     8401 ctttccaagg taaatcttgt ggatgaaaga acaataatgt taataggaaa agtaatggac
     8461 ctaatattag tccaataagt tgtcccgtac taaacggttt ctttttcttt gattttgaaa
     8521 agaatgttaa ataccttttc gatatcattg attgatgttg attgaaataa gccataaatt
     8581 tccccctcat gatgattatg acaagctact tcaactaact atattagagt atagaaagtt
     8641 gtctatcttg tattatcatt accgtcaaaa tacccctttt atcagataat tgggtttatt
     8701 atagcatatt tataccgttt tatgaatgcg ctttcaaaaa ttatccagta atcttaatcc
     8761 taatgtcatg cgaaactttt tactttttta tttcatctac attgattgta gtaacttgaa
     8821 tacccgcttc aacaggactt aaaaacactg tatcaatatc ttccaaatga attgattgag
     8881 tcgaattatt gaatgtattc ccaacgatta atacatcttt atgtttaaca ttattataat
     8941 tacgtacatg tatcacatct ttagacattg accctttaaa ttcatttcca attatattca
     9001 tatttattcc tgcttgggaa cctaagtctt ttcctgtcat cacatctgca gcatttttac
     9061 catctctaac tcctaaatat ctaataccac cctcacagtt aataaactta ttattaataa
     9121 tgaaagcatc tttatacttc aagggagtta atgcataacc ttgtatatct tcaaatatat
     9181 tatttctaat atgaatattc tcatagtaac gattatgtct acttgcatgt gagccaatag
     9241 cacgattcca acttcccatt tcgggcaatt ctgaaggtcc aaaataacaa tcttcgataa
     9301 tgacattttt cgttatcgta ccatctgtcg ttccgaattt tggaaaagca ccaggtactt
     9361 gaatgtctaa ttgtattgct tcagaataaa aacgttcgcc actatagtca acgaatcctt
     9421 caaatgaaca gtttttaata tagagtccat taatcccaca agcatcaatt gcatgaccac
     9481 tcactacatt cttaatggtt acacctatta attgaatatc ttcagcatgt ccaatgcaca
     9541 tagctgtatt attataagga tacgatacgc cattcatatc aaacttgccg cctttaatat
     9601 gaatatgact gtgtccatta taaccacgat aaaaaccaaa gcgatgacca ttttttaata
     9661 acggaccaga atgacatcga cgtaaaatag tttcattatc caacaaaagt gttgtattgc
     9721 catatatcgt taatggtttg caaatatcat acgtcccttt cggtatataa actgtcgttg
     9781 gtttacatct tccatgattt aacgcacgtt gtattgctcg agtatcttta gttttgttta
     9841 tgcctgttaa cccataatct ttcatattga tatacataaa tcgtcatcct ttgtctattt
     9901 tctaattact catatattaa ccaattattc aaattataat cgtgttaggt tgatttagca
     9961 gatagtcata aatctcgtga tgccatctat tagcattttt atattcaaaa catcaacact
    10021 tgatattagt tctaagtttg tcacatttag tattctatta cagctatttt aaagctatcg
    10081 taaaaacatc ttataatatt tattgattgc gtgatttgct cttccaaaaa agttgattaa
    10141 tctatatact taatctatat ttatatatta accattaggg ttaaaaatta ctctagcatt
    10201 tatgaataga tgggagttta ttttattatt atataggaga gatgttgaat gacacatcgc
    10261 gcactattag ttgttgacta ttcatatgac tttatcgcag acgacggctt actaacatgc
    10321 ggtaagcctg gacaaaatat tgaagatttt attgtttctc gtatcaatga ctttaattat
    10381 tatcaagacc atatattctt tttaatggat ttacattatt tacatgacat tcatcatcct
    10441 gaaagtaaat tattcccacc acacaatatc gtagatacaa gtggtagaga attatacggt
    10501 aaagtaggta aattatacga aacaattaaa gcgcaaccta atgtacattt cattgataaa
    10561 acgcgctatg attcgttctt tggtaccccg cttgatagtt tattgagaga aagaagtatt
    10621 aatcaagtcg aaatcgttgg tgtatgtacc gatatttgcg tgttacatac agcaatttct
    10681 gcatacaact taggctataa aatttcagta cctgctgagg gagtggcttc atttaatcaa
    10741 aaagggcatg aatgggcact tgcacatttc aaaaactcat taggtgcaga ggtagaacaa
    10801 cacgtttaaa tcgtgctaaa ataattataa agaatacaat atacaaggga gatatttgac
    10861 aatggctaaa acatatattt tcggacataa gaatccagac actgatgcaa tttcatctgc
    10921 gattattatg gcagaatttg aacaacttag aggtaattca ggagccaaag cataccgttt
    10981 aggtgatgtg agtgcagaaa ctcaattcgc gttagataca tttaatgtac ctgctccgga
    11041 attattaacg gatgatttag atggtcaaga tgttatctta gttgatcata acgaattcca
    11101 acaaagttct gatacgattg cctctgctac aattaagcat gtaattgatc atcacagaat
    11161 tgcaaatttc gaaactgctg gtcctttatg ttatcgtgct gaaccagttg gttgtacagc
    11221 tacaatttta tacaaaatgt ttagagaacg tggctttgaa attaaacctg aaattgctgg
    11281 tttaatgtta tcagcaatta tctcagatag cttacttttc aaatcaccaa catgcacaca
    11341 acaagatgtt aaagcagctg aagaattaaa agatattgct aaagttgata ttcaaaagta
    11401 cggcttagat atgttaaaag caggtgcttc aacaactgat aaatcagttg aattcttatt
    11461 aaacatggat gctaaatcat ttactatggg tgactatgtg actcgtattg cacaagttaa
    11521 tgctgttgac cttgacgaag tgttaaatcg taaagaagat ttagaaaaag aaatgttagc
    11581 tgtaagtgca caagaaaaat atgacttatt tgtacttgtt gttactgaca tcattaatag
    11641 tgattctaaa attttagttg taggtgctga aaaagataaa gttggcgaag cattcaatgt
    11701 tcaattagaa gatgacatgg ccttcttatc tggtgttgtt tctcgaaaaa aacaaatcgt
    11761 acctcaaatc actgaagcat taacaaaata atactatatt actatctaat tatagacatg
    11821 ttgtatttaa ctaacagttc attaaagtag aatttatttc actttcaatg aactgttttt
    11881 tatttacgtt tgactaattt acaacctttt caatagcagt tttcattcct ttagctatcc
    11941 taacccgtag attagtgatt tctatacaat tccccttttg tctttacatt ttcttaaaat
    12001 atttgcgatg ttgagtataa atttttgttt tcttcctacc tttttcgtta tgattaaagt
    12061 tataaatatt attatgtaca caattcatcg ctctattttc aactttcaat atatataatt
    12121 cgaaagacca tttaaaatta acggccacaa cattcaaatc aattaatcac tttttccaaa
    12181 ataatcatat aaggaggttc ttttcattat gaatatcatt gagcaaaaat tttatgacag
    12241 taaagctttt ttcaatacac aacaaactaa agatattagt tttagaaaag agcaattaaa
    12301 gaagttaagc aaagctatta aatcatacga gagcgatatt ttagaagcac tatatacaga
    12361 tttaggaaaa aataaagtcg aagcttacgc tactgaaatt ggcataactt tgaaaagtat
    12421 caaaaatgcc cgtaaggaac ttaaaaactg gactaaaaca aaaaatgtag acacaccttt
    12481 atatttattt ccaacaaaaa gttatatcaa aaaagaacct tatggaacag ttttgatcat
    12541 tgcaccattt aactatcctt ttcaactagt gttcgaacct ttaatcggtg ctattgcagc
    12601 aggtaataca gcaattatta aaccatctga gttgacacca aatgttgcac gagtgattaa
    12661 acgattaatc aatgaaacat ttgatgcaaa ttacattgaa gttattgagg gaggaattga
    12721 agaaacgcaa acgttaattc acttaccttt tgactatgtc ttctttacag gaagtgaaaa
    12781 tgtaggcaaa atcgtttatc aagctgccag cgaaaattta gttcctgtga cattagaaat
    12841 gggcggtaaa tctccagtca tcgttgatga aacagcgaat attaaagttg ccagtgagcg
    12901 catttgtttt gggaaattca ctaatgccgg ccaaacatgt gttgcaccag attacatttt
    12961 agtacacgaa tctgtaaaag atgatttaat cacagcccta tcaaaaacgt tgcgtgaatt
    13021 ttatggtcaa aatatacaac aaagtccaga ttatggccgc attgtaaacc ttaaacatta
    13081 tcatcgtctg acatcattac ttaacagtgc acaaatgaat attgtatttg gtggtcatag
    13141 tgatgaggat gaacgttata tagaaccaac attgttagat cacgttacaa atgattcagc
    13201 aattatgcaa gaagaaattt ttggtcctat cttaccgatt ttaacgtatc agtcattgga
    13261 tgaagcaata gcctttattc accaaagacc aaaacctttg agtttatatt tatttagcga
    13321 agatgaaaat gctacacaac gtgtaataaa cgagttatca tttggcggcg gcgctattaa
    13381 tgatacattg atgcacctag cgaatcctaa attaccattt ggtggtgttg gcgcctcagg
    13441 tatgggacgc tatcatggta aatattcatt tgacactttt acacatgaaa aaagctacat
    13501 tttcaaatct acacgattag aatcaggtgt ccatttacca ccatataaag gtaaatttaa
    13561 atacatcaaa gctttcttta aaaattaatt tttgagacaa aaataaacca tgcttccata
    13621 atttgatgtt cttgaaaaca atatatttca aggtatacac aaattttttg gaaacatggt
    13681 tttcttttta tcatgattaa ttaaagatta aaatatgaca catacacctt cagatgctac
    13741 acgagtgtta tcacatagcg taattttacc tgtttcttta tttctttcga aaacagttac
    13801 aactgaatct ccttgctcat gagcacaaac taaaagatca tctgatgagg caatattaaa
    13861 atctcttggg aattgaccac cactttcagt aattgttact agttctaagt gttgaccatt
    13921 atcaagaact ttaaaaattg caatgctatc atgccctcta ttagatacat ataagaattg
    13981 ttgatcatga gataaacgca ctgctgcaag tttagtatct ccatcaaagt tttcaggaat
    14041 tgttaaatga cgctcgagct cttcaaattt accgtcatta tattctgcaa cgctcacagt
    14101 atttgataat tcgtgtacga cataagcaaa ttttccatta tcatgaaatt caatatgtct
    14161 tgtcccatca ctatctttaa ataaagattc tttataaaat tcaaaaccgt tgtcatcaaa
    14221 tttataagta acgatacgat cagcacctaa atctgttact gcaacgtact taccatctgg
    14281 agtttgatta atataatgtg cgtgtggatg atcttgtctt tcatgtgtac ctgttggaaa
    14341 atcatgcgca agttcttgaa ttaaacgtat aatttcacct gtgtccgtat ttaattcata
    14401 catacgtatg atgccagcac catataccgc ttcaaataaa tatcgtttat cttctgaaat
    14461 cgatacataa caacctgtac cagcttttga agacaaacat ttatttaata aatgcaattc
    14521 accattgtca tcaattttca aactcgcaac accgcattgt tctccttctt tgttgattcc
    14581 atataaaact tcgttattac gcaccaaata tgtagacgct tctaattcaa atcctgtttc
    14641 taataaatca atacgtgact gattttcgtt taattcaaaa cgataaatcc ctttaccatt
    14701 ctttttagtg taagaaccaa tatatccatt tgtcattact tttacccctc catgttagtc
    14761 ttattttcat tttattaaat tttagctaga attaacaatc atttatcatt cccttaaagc
    14821 taaaactgtt taactacttt aaaagaacta tttgctggtg atatgtcatt attcgatttt
    14881 aattaatccc tcttttttac ttacccgagc attaattatt aacgcgttta tgcttccaca
    14941 aaatttttgt tgcaaaatag aacacatgtt cgtataatgt aattatcaga ttggggtgaa
    15001 ttaaggatga caagttatga agaagaaact gattatcgta acgttccaag agaattacta
    15061 gataacaata tacctatggg aagaggcatg attaaatggg ctccttttgt tatatgtact
    15121 aatctcataa aaaatctaat ttaatagggt tcaaacataa tttatatatg tattatgttt
    15181 gaaccctata tctatttatt ttttactaat atcatttaaa atgtctcgca attcttcaat
    15241 ttctttgtta tttaattctt catttttcgc aaaattaagc actaaacttt tcatatcccc
    15301 tccatacaat ttattaagaa aggttttagc agttttcatt ttaatatcat cttctttaat
    15361 aattgatgag taaaaataaa tattattata tttatagcgt tttataatct cttttttata
    15421 tagtcttgta attaatgttc taatcgtctt atcgctaact tctttatttt tttgaatctc
    15481 aactacaatt tcattagctg atactgattt tttattccat attatattca taacatccca
    15541 ttcagccata gatatttcaa cttgcttatt ggccatttaa aacacccatt tcttttaata
    15601 ttttttcatt tatgaattta gcattttcac cagaggcttt ttcatctgat aaatgtgtag
    15661 aaaaataata cttatcatga tttgtaatta cgtaacctac aaaccaccca ttattatatt
    15721 tcccgtttac tatacctgta cctgttttcc catacaattc ataattttca ttttttctaa
    15781 tcaataacga agaagataat tgtttctttt catttttact aaaatggtta ttttgttcca
    15841 tcatattttt taaaactatt acttgttcaa gattagatat tttcaaacta tcttccaacc
    15901 aataggcttt ataacttccc aaatttttat taccataatt taattgcttg agttgatcag
    15961 aagtatagtt ctttgataat tgattactaa tacgttcgaa gtaccaatta actgagtttt
    16021 gtattgccgt atttaaatct tgatccttat tccaagaatc gaaaggataa tgattatgat
    16081 tccatgacat acgtgaattt ttatcactta taatatgacg gtcaagtcca aacagtgcta
    16141 aataaatttt gtaagttgaa tcaggagaat atctttttct actttctttt tcattataaa
    16201 tatagtattt gtctttcttc atgctgtaca taacgaaaga tccactattt gaaccaaaat
    16261 ttttactttc atctaaaatt tggtaatcac tttgtaaggg ctttttataa ttataatcag
    16321 ttaaggattg ccccattaaa aactggcttt gaattatcat gataaaaaaa gtaaaaatac
    16381 aaataaaaat tagaattaat tttgactgct tcttcagatt gccttctttt atattaatta
    16441 atcttctttt gagtaatgat tttttaccat taaatgaatg gcttaatata tttttgttga
    16501 aaaatgaggt ttttaatacg gaatccataa tagcttccgc ataagactta aattcatttt
    16561 tgttaatatt attaaggaca aatctatcgg cttctacttc attgtcatga atcatctttc
    16621 tttttacaat ttgtattagt ggattataac tcatagcaat agaaaaaata tgaaagataa
    16681 ttaaatgcaa agtatctctg tttttagcat gagcatactc gtgcaatatt atatatttta
    16741 gttttttgtc atttatactt ttaaaatata aactgggaat caaaataata tattttccat
    16801 accaaaaagt tattggagaa tgaatagatt ctgcttttcg aatcacaata tttcttttat
    16861 attgatgatt gaaaagtatc ttgtttattt tatctttttc tttttcattt agataaagtg
    16921 actgtttctt taaatactta agatataata aagaatttaa aaatttaaaa cttaaaataa
    16981 taactaaaac tatccaaata actgtgcaga tattatctat tgaatcccaa ttaaacttat
    17041 ggatgtctgt tgtaaactca tgaacaggtt tagtggtatt tatgttagga ttcaaattgt
    17101 gtgaattact ttcaactgtg ggctcttgat tattcaaatt attaaattta aaaaaagaga
    17161 atttaatagg gatgaaagga attaatcctg caagaagagt tagataccaa actttataat
    17221 ttaacatata attaaaatag cgttttaata tgtacctaaa aaataataac aatagaaata
    17281 taaaacaaaa gcttactatg ctcattatta ataacttagc catttcaaca ccttctttca
    17341 aatatttata ataaactatt gacgcgaata ttacaattgt aatattattg atttatagaa
    17401 attacaactg taatattgga gggtatattt tgaaaaagtt aatactttta attgcaattg
    17461 ctttagtttt aagtgcatgt aattcaacca gttcacatgc taaagagtta aataatttag
    17521 aaaagaaata taatgctaat attggtgtct atgcattaga tactaaaagt ggtaaggaag
    17581 taaaatttaa tgccgataag agatttgcct atgcttcaac ttcaaaagcg ataaatagtg
    17641 ctattttgtt agaacaagta ccttataata agttaaataa aaaagtacat attaacaaag
    17701 atgatatagt tgcttattct cctattttag aaaaatatgt aggaaaagat atcactttaa
    17761 aagaacttat tgaggcttca atgaagtata gtgataatac agcaaacaat aaaattataa
    17821 acgaaatcgg tggaatcaaa aaaattaaaa aacgtttaaa aaaattggga gataaagtaa
    17881 caaatccagt tagatatgaa atagaattaa attactattc accaaagagc aaaaaagata
    17941 cttcaacgcc tgctgctttc ggcaagactt taaataaact tatcgcaaat ggaaaattaa
    18001 gcaaaaaaaa taaaaatttc ttacttgatt taatgttaaa taataaaaac ggagacactt
    18061 taattaaaga tggtgttcca aaagactata aggttgctga taaaagtggt caagcaataa
    18121 catatgcttc tagaaatgat gtagcttttg tttatcctaa gaaccaatct gaacctatta
    18181 ttttagtcat ttttacgaat aaagacaata aaagtgataa acctaatgat aaattgataa
    18241 gtgaaaccgc caagaatgta ataaacaaat tttaatagtc taaatgtact acaaaaactg
    18301 atataattct atctttcata aaacttcata atatcgaaga acatatatat tatttataat
    18361 tatatatgtt ctattttttt atgttcacat tttttaaaag agaaaatact attaacatac
    18421 caaacatcat aattccagtt actataatta taaatgctcg ataatcttac tcaacttttc
    18481 catatagaaa ataacttatt ttaaattaat taattaatca cattaaaaaa ttatataatc
    18541 cttgccaaaa aagaacatac gttctacaat gatgatgagg tggtaaacat gattccaaat
    18601 cctaatgcgc cagatgaata taaatatgaa actgattata gaaaaatacc aagaaaatat
    18661 ttaaacccta aaatccctca agggcgtgga aaaatcaaat ggcaagcttt tgcgactctc
    18721 ccgcaacagt ttgaaatttt agaacaaatt ataaaggatc aaaataaaat agaaaaacca
    18781 ttgctaacac atgattcatt agataattta gatcagattt ttcaaataaa aattcaaaat
    18841 gatgagttat gtacaatctc atattgggaa gacggtaaca tttcaaaata tacaggaaaa
    18901 attttaaaaa aggatgaaat tagtaataca ttttcctttt ctgatactaa caataatata
    18961 tataatttaa ataatgctaa cgtttgtagt atcacttaac actatttaaa cttttaaaaa
    19021 gtttaaatag tgttcttata attacctttt tcgaaatact tctagcaaaa tcattataag
    19081 tatcatactg atattaaata tattattttt atcactgaaa aaaatttcta ctacttggaa
    19141 aattgataaa agacttatta ttaacaaaag tttatttaat ttttcgcttc tttcttcttc
    19201 cttatatgac tgctccaatt caattagtga taattcttct ttgtttaatt cttcaatata
    19261 ttgtggagat ttaaaatcat tattattctt tagatacaaa tataaatata aactaggtaa
    19321 aagctctgtt ctataaataa ttgcaataga tctcttgaaa tttagtatcc attgtctaaa
    19381 atatagggct tctttttttc catttaaatt atattgttga tacccataca attgtaattc
    19441 atttaacata gtttctaaga aaaagaaatt agcagaacta ttgaaataaa gtgccatttg
    19501 agaacatttt tctaaaacat cttcttcttg ctcatccatc caaactacaa aaacagatgc
    19561 tttattcaag aagtttatat aattatcgct ataaccatct tgcctaaaat gtttactttt
    19621 tccttcaaat gaaaaaatag gcgcatttac taaccactct tgaatcttca cgtcctcata
    19681 gaaaggatca tcagtaaatt cattttcatt agctaaataa taaacttcaa aacccatatt
    19741 tttagatact ttatattcaa aaatatcctt actattaatt tttataattc tattaatttg
    19801 tctagaaaca tctctaatat ttttacaatg tctattacca tacaattgat actgtctttg
    19861 tctattattt ttttcttgat taattagagg atatatctta tcttttacac atgagaaagt
    19921 actatacaag tcgcttccaa agtttatact ctctaagtct tcaatgattt ctataacaaa
    19981 tgtaccattg ttaaatatag ttaaggttaa aaaaaataaa ttcaaagtgt tctcatgagt
    20041 aatttcaata tgtggagtga atatatattt tttatatttg tatccatgta tattattttg
    20101 atcaaacaat ccatcattaa tatttgcatt agtagcaaat ttttctaaaa atttttctag
    20161 tttttcgtga tattcttttg tatgtctttt atatcttcca ggtaaaatcc aataatcata
    20221 atttaccggg gggttgtcag gagataaaat gcgtataact ccttttacat tcaatagaat
    20281 ttcctcaatt ttttcaaacc aagggttcat gttattatct tttatattta tagcagtagc
    20341 atattgatat gttactttat agccttgtaa tttattaata tcttctagaa tcttttcaat
    20401 tttattgtcc attattttaa atatcctcat acagcctaga atataaaacg ctaatttctt
    20461 ctttcaacat ttttttctct ttttctaaag aatttacttt tctttttaaa gaggagatta
    20521 acgcatcttt accgtcattt ttatttttat gaacaaatat ttctctgttt tgttcacgta
    20581 acttctctat tttctttcga attttattat ttttatataa tgtagctttc gaaacttttg
    20641 aatgtttaga aacgatgtta aagttaattt ctttattttt ttgaactaaa tcatttattg
    20701 ctttatcaac ttttttctcg gttttaactt ttcgttctgc atgcaactgt tttaattgtt
    20761 cttgtctatt aaatttttcc atcttaacct ctatactttt attctattac ttctcccgaa
    20821 tataat
//
LOCUS       DAPIDM010000033        20569 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000033 DAPIDM010000000
VERSION     DAPIDM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.033"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(88..927)
                     /locus_tag="RJE42_001095"
     CDS             complement(88..927)
                     /locus_tag="RJE42_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV5876526.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(929..2197)
                     /locus_tag="RJE42_001096"
     CDS             complement(929..2197)
                     /locus_tag="RJE42_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV5876527.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(2200..3471)
                     /locus_tag="RJE42_001097"
     CDS             complement(2200..3471)
                     /locus_tag="RJE42_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDV5876528.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(3484..4581)
                     /gene="ugpC"
                     /locus_tag="RJE42_001098"
     CDS             complement(3484..4581)
                     /gene="ugpC"
                     /locus_tag="RJE42_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDV5876529.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENIAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(4966..5544)
                     /locus_tag="RJE42_001099"
     CDS             complement(4966..5544)
                     /locus_tag="RJE42_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDV5876530.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNIKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            5753..6379
                     /locus_tag="RJE42_001100"
     CDS             5753..6379
                     /locus_tag="RJE42_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV5876531.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPEDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDELMKVSRMSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            6569..7342
                     /locus_tag="RJE42_001101"
     CDS             6569..7342
                     /locus_tag="RJE42_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5876532.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     NTYPIKNLETLYEIDGHDNPYYKNNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQN
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(7655..9661)
                     /gene="ggt"
                     /locus_tag="RJE42_001102"
     CDS             complement(7655..9661)
                     /gene="ggt"
                     /locus_tag="RJE42_001102"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV5876533.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLTLNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKHYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIIQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKECGGTITLEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HIDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHELPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVAGGINEIAPYKRPLSNMAPTIVMHHGKLILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLLSPEIYDNDTH
                     RVYFNDVQLPLLAEQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIA
                     WVARKKGYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(9699..11474)
                     /locus_tag="RJE42_001103"
     CDS             complement(9699..11474)
                     /locus_tag="RJE42_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5876534.1"
                     /translation="MKKIISIAIIVLALVLSGCGIPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FTKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(11491..12654)
                     /locus_tag="RJE42_001104"
     CDS             complement(11491..12654)
                     /locus_tag="RJE42_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876535.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVVFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGICIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(12660..14138)
                     /locus_tag="RJE42_001105"
     CDS             complement(12660..14138)
                     /locus_tag="RJE42_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876536.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VISIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTKVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            14095..15687
                     /locus_tag="RJE42_001106"
     CDS             14095..15687
                     /locus_tag="RJE42_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876537.1"
                     /translation="MSNLLEVNSLNVQFNYEETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYKSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKALRREIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(15893..16756)
                     /locus_tag="RJE42_001107"
     CDS             complement(15893..16756)
                     /locus_tag="RJE42_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDV5876538.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTMLNKLLLGRHSVNTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TSRNTLSEVQKPKENNSLLNSKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHF
                     LKDANDPPHFT"
     gene            complement(16956..19745)
                     /locus_tag="RJE42_001108"
     CDS             complement(16956..19745)
                     /locus_tag="RJE42_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDV5876539.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
BASE COUNT         6641 a         3791 c         3083 g         7054 t
ORIGIN      
        1 attaactgtg gctaatttgt aagagccact aacacaataa attcattagt ggttgtttat
       61 catttctgcc ccgctcctaa tttcaaacta accttttgtc gcacctgttg ttaaacctga
      121 tactaaatag cgttgcaaga acaagaatac gattgctata ggtactgcaa tcataattgc
      181 ccctgctgca aacactgtga aattatttgc atacttatca ttaataaagt tgaacaatcc
      241 aactgctaat gtgaattttt caggacttct taatagtatt ttaggtaata taaagtccat
      301 aaatggcccc ataaaattga acaaagcaac aactgctaaa atcggcttag ctaatggaag
      361 cataatttgt aagaaaatac gcatatgccc tgcaccatca attttggcag attcatcaag
      421 ttcttttgga atcgtatcga agtaaccttt cactaaaaag gcattcatcg gtattgatcc
      481 accaatatat accagtgtta gtccaaataa agaatctaat aatccaattg tatttagcaa
      541 aatatagatt gcgaccattg ccattaatac agggaacatt tgtaaaatca aaaatgtaat
      601 cagcccgtat ttacgaccaa caaagcgata tctagaaaaa gcatatgctg ttaacgtgac
      661 aaatatcacg ctaaacagtg catttgcaga tgctacgata agcgtatttt tataccaagt
      721 caggtattga ctactgtcat cgaatagtaa gaatgcataa tttttaaatg ttgcattgtc
      781 tggtatcatt ttggcaccat acaagttcgt acctggatta agggaaatgc caaatgtcca
      841 cagtagtgga tataaaatga tgacaaacat catcgctata aaactgtaaa tgccgattgc
      901 ttttaatatg tttttcttct ttgtcatctt ataaacctcc ctcatcttta aacgtacttg
      961 tacgtctgaa ttgaataaat gcgacaatag caacaataaa tccaataatt aatgacacaa
     1021 ctgcacccat gttaaagttg ttaaactcaa atgtcagatt atacacccaa gatatcaaga
     1081 tgtcggtact accagcattc tgccctgaca ctggtggacc acctttatta aatagataaa
     1141 taagattaaa attattgaaa ttacctgcat attgcataat taacaatggc gctgtggcgt
     1201 aaatgacatg cgggaatgtg atgtttctaa acttttgcca actagacgca ccatccatat
     1261 ctgctgcttc gtaccaatct gatgaaatac tttgcagtac accagtgaac agtgcaaaga
     1321 caaatgggaa accaagccat acttgaatgc caattaatgc cacttttgcc caaaatggat
     1381 cacttaacca tgctggtgca acacctaata aaggttgcaa tatatcatta tttatcgcac
     1441 caaattcatc attaaataac gctacaaata ttaaaattgt cacaaatgat ggtacggccc
     1501 aaggtaggat taacacagta cggataaatt tcttaccttt gacgacaggg tgatttacaa
     1561 taattgccaa aaacagccct aatgcaattt gaagtgtcgt tgcaacaagc gtccatacta
     1621 atgtccaagt aataacactg aaaaatgttt tacgccaaac gccaatcgtg aataacgttt
     1681 taaagttatc taaaccaacc cattctaatg tatgtctcgg aggcgcgttg tataaattgt
     1741 agtttgtaaa tgctactcca aacataaata ttaatggaaa tacaactaca aatatcaata
     1801 agaatgtacc tggtgagatt agtaagtatg ggaacgtctt gtcccatgtt gcaaccatgc
     1861 gagccttcgg atcctttatt tcctcattgc gattaaatcg ttcagcatta cgatatgcat
     1921 ctaaaatatt aatgacatat agcatgattg cgaaagcaac aagtaagata gaaataatac
     1981 cttgtgcaag taagacacga gaatcgtcta acttaggtac tgtccctaat gtgaacaatc
     2041 cccaaaaacc aatatttaaa aagctataaa atacagaaat aaagctgatg aaaaagataa
     2101 aaaatatcgt acctttaatg ggtcttttat tataaaactg tcccaaacca ggtataacag
     2161 ataataatgc cgctaattta gggttacgtt tcgtcataac taatctcctt tcttatcatt
     2221 ttgtgacgga tgaagaatct taatattttg cgttatatca ttcgtcgcct catctaacgc
     2281 ttgtttagga ttcttaccat ttgagataaa tatgcttgca ttgcccatcg gttcccaaac
     2341 ttgtcgcatt tcaggaatat taggcatcgg ttcagcatga cgtgcttgct tttcaaacac
     2401 ttttaaattt ggattagatg atttcacgtc aacacgtcca gtaatttcgc tcatttcatc
     2461 tgtatatttt tgtaatgtat ctttactagt gatatacaac attaaatctt tagcccaata
     2521 cttatgttta ctatattcag ataaatacca accacgtaca ccaagaaatg gtttcatagg
     2581 tttgccacca tctgtaggca atgttgttac tcctaaatct ttaccaaacg tttcttgata
     2641 ttcattaatg ttccacggtc cagtgacaaa ttgtcctact tttccttctt taaaaagacc
     2701 aatcatgaca tcatgtgttg ctgccttagg aagataccct ttgtcgtacc atttttgtaa
     2761 tcgttcagca ttcttgacga catgttttga atttaatcct agctgatgaa tatcatattc
     2821 actgccattt ttcttgaaaa tataatcatc attgccgaat aaaaacggat aattaaaata
     2881 gaaattttta gcatcaaata gcataccgta ttgtttcttt ttactatcag ttagtttggc
     2941 agcattagct tcaacttctt ctaacgtttg cggtgcattt ttcactaatt ttttattata
     3001 gaaaagtgcg gttgtttcaa cgatagctgg caatgctagt tgcttattgt cataattcat
     3061 cgctttaagt gcttgcttat tgaagccttt caactcatct tttgataatt tgatttcagc
     3121 agctaagcct tgtagatagg cacttccagt attatcatgt gctaagaaaa agatatctgg
     3181 accttttcca gcaggagcgt ctagagaaat attttctagt tgatcatttt gaccaatatt
     3241 tacaagcttt acttttatgc cagttttttt agtatattga tccgtaattt ttttataaaa
     3301 cgccatttgc ttgtcgccat ccacccacat cgtaagttgg ttaggcttgt ctttagaatt
     3361 atctttattc aatgctttat caatatcttc tttcgaacga ttagggccac atgcagttac
     3421 gattaataac attaccacgg ctaacgtgat acattttaaa attttagaca tgaatactcc
     3481 cccttagacg atacgatttc ctgttttttc atcaaaaaag tgacacttat tcatatcaaa
     3541 tgctagtgta atcttgtcat tcgccatcac ttgtgttctt gaatctaatt tagaaatcaa
     3601 ttccattcct tggaatgtgc tatgtaccat aatttctgaa cctaacagtt cggatacaac
     3661 tacttcagat tcaaattgtg tctctggaga ggtttgaata aatattggtt cttcatgaat
     3721 gtcttcagct cgaataccta aaataatttc cttgtctaag tagccagcag cttttaactt
     3781 ttcaaatttt ttattatgta atttaaaatg gtgtgttcct accttcaatc cgtccatttc
     3841 aactgtagca ttcaacatat tcattgctgg tgagccgata aattgcgcca caaatatgca
     3901 attaggggca tcatatattt ctctaggtgt gccgacttgc ataatgtcgc catctttcaa
     3961 aacaacaatt cgactagcca tcgtcaatgc ttcagtttga tcatgtgtaa cataaattgt
     4021 cgtagtatta agtcgcttat gtaatttcaa tatttctgta cgcatttgta ctcgaagctt
     4081 cgcatcaaga ttcgataatg gttcatccat taaaaagact ttcgcatccc taacaatagc
     4141 tctgcccaaa gcaacacgtt gacgctgtcc accagataac gcttttggtt tacgaccaag
     4201 atactcagtt aatcctaata tttctgctgc ttcattaact ttttgttcaa tctctttttt
     4261 attcacttta cgtagcttta gcccaaatgc tatattttca aaaacagtca tatgtggata
     4321 taatgcatag ttttgaaata ccatcgcaat atctctattc tttggttcaa catcgttcat
     4381 gcgttcccca tcaatataaa aatctccaga tgtgatagac tctagtccag caaccattcg
     4441 taaagttgtt gatttaccac atcccgatgg tccaacaaat acaatgaatt ctttgtcagt
     4501 aatatgtaga ttaaaatctt tcactacagt attgttgtta tcatacgtct ttttaatatg
     4561 ctctaacttt aattctgcca tgttatgcct cccatacttt gtttacaatt tgattgtgca
     4621 atacacttcg tgaaaacgtt tgcacaaata cttacataaa tcatatagtc actcattatt
     4681 aatgtcaatc ataaattgta aacgtttaca ttactgtcag aattaaactt ttatacagaa
     4741 aattgatata gcacacaact atttttagtg tgacaataat gattttttac gtgcgatatt
     4801 tcactgccaa tgctaacaac cttgtcttac attttttaag taatatctaa atcaaacaag
     4861 tatgacttag catttcgatt ttcattgaaa aaagcaacca ttatgtaagt tttatactca
     4921 cacaatgatt gcttgatttt ttagactgcg gtcactcttc tataactaca aatcatatag
     4981 gctacgttcc ccatcaggta actgatcgat aaacggttta ggatcttctg catatgcatt
     5041 acctacgcca cccttcattc tttgaaaatg taaatgtgcg cctgtcgttt gtttacctgt
     5101 attgcctgaa tatgcaataa tatcacctgc tttgactcga tcacctactt tgatattata
     5161 tttgtctaag tgtagatacc actggtgata ttctccattg tcttcggcaa tctgtaatac
     5221 ctttccgcct aattcattat taaagatacg tgttacttta ccattcgtcg gcgctttaat
     5281 tggtgtacct tttggcagtg caaaatcaat accataatgc ttaccatcaa atggtgaatg
     5341 ttgatattta ccaaaagtct cagtaatccg actccctcta aaaaagtctt cccattttga
     5401 gtgtccttgt cctttatgat tttgtgtcaa tttatcggta tattggttaa accatttttg
     5461 tatgtcatct atataaataa tcgtaaataa tagtgaaaga aaaatgataa ttgttgccaa
     5521 aatacgtttt ggtcgctttg tcatagtggc atccatcctt catatatttt gggtattaag
     5581 tatatattct aaatcaacta tttcataatt aaatgcactc atactttagt agtaccgcca
     5641 aatatagatg aagaaaatga tggtaatttg aatattttga ttgatattaa attttatatc
     5701 gagtataata attatactta agaaaataac agaaacagag gagaattttc aaatggcaaa
     5761 agtattatat attacagcac acccttttaa tgaactggta tcaaattcta tggcggctgg
     5821 taaagcattt atcgaaacat atcaacaaca acatcctgaa gatgaagtga aacatattga
     5881 tttatttgaa acttatattc cagttattga caaagatgta ttaactggtt ggggaaaaat
     5941 gagtaatggc gaaacgttaa ctgatgatga actaatgaaa gtttcaagaa tgagtgacat
     6001 tttagaagaa tttttaagtg cagataaata tgtatttgta acacctatgt ggaacctttc
     6061 tttcccacct gtagttaaag catatattga tgctatttca attgcaggta aaacttttaa
     6121 atattctgct gaaggaccac aaggtttatt aactgacaaa aaagtattac acattcaatc
     6181 acgtggtgga tattatactg aaggacctgc tgctgatttt gaaatgggtg accgttattt
     6241 aagaacaatt atgacattct taggtgtgcc atcatatgaa acaatcatta ttgaagggca
     6301 caatgccgaa ccgcataaaa ctgaagaaat taaagcaact agtattaata atgctgaaaa
     6361 attggcaaca acattttaat tgttatctac actgtgtgac taatcaaacg tcatacagtg
     6421 ttttttattg tatacctttc ccgtttttta taaatatcta ttattttcat aaatttaaaa
     6481 tttgatattc actttaatac tgatgtaaat atttttaaaa tatattatta tagtgttaat
     6541 gaagtatttt aagaaacggt gtgaatgtat gaaagcacat aaaattttct ggttaaactt
     6601 agcagcaatt attattattt caattgtagt cagtggcggt atgtttttgg caatgaagtg
     6661 ggaacaaatt catttgaaag atggccttaa aaaagtacta aatacatatc ctattaaaaa
     6721 cttagaaacg ctttatgaaa ttgatggtca cgataatccc tattataaaa ataatgatca
     6781 agacacatgg tatatagaat cctcttattc agttgttgga tcagatgagc ttttaaaaga
     6841 agaccgtatg ttgttaaagg tagataagaa cacacataaa ataactggtg aatatgacac
     6901 aactacaaac gatagaaaaa atgctactga ctcgacatac aaaagttatc cagtaaaagt
     6961 agttaataat aaaatcgttt tcacaaaaga cgtaaaagat cccgcgctga aacaaaaaat
     7021 cgaaaacaat caatttttga ttcaaaatgg tgatttaaca agcattttaa atagcaatga
     7081 tttgaaagta acacatgatc ctaccactga ttattataat ttatctggta agttgtcgaa
     7141 cgataatcca aacgttaaac aattaaaacg tagatataat attcctaaaa acgcatcaac
     7201 aaaggtggaa ttaaagggaa tgagtgattt aaaaggcaat aatcatcaag atcagaaact
     7261 ttatttttat ttttcaagtc ctggaaaaga ccaaatcatt tataaagaaa gccttactta
     7321 taataaaata agtgaacatt aatatttatg atgtaactat aaaaatatcc aaatcattta
     7381 ttagcattta acattctaaa atcccacatt ataaaaacac ctcacataac tcgttcaact
     7441 gtaccaaacc acatcacgtt agattttaga ctaactattg tgatgtacat taaaaacgaa
     7501 tttgtgaggc gttgtatatt ttacaaaggt gactagcgtt tcgtatagca tttgcaacat
     7561 tactacactc aagcgtcact ctaaagttcc aaatcgaatc ctttcattca acaaaaaccc
     7621 ttatcaacta caaacttcat atcaagcgca taaactatct tgtgatacta tctcgatcat
     7681 atctatagta tgcatttgtg ttccgtttca ctgaagtata tgtatcatca gttaagtata
     7741 aaccgtcatc cttcaatgtg acttgataac ctttctttcg tgctacccag gcaatatcta
     7801 tataattttc tcctgcgttt tcataacttc ttaaatcttc aatatgtgca ctaacttcag
     7861 agaagatgat tctaacgaca ctttcatcaa cccaatattt gtcatgcatc catcgcactt
     7921 gttctgccaa taatggtaac tgcacatcat tgaaatatac acgatgggtg tcattatcgt
     7981 atatttcggg actaagtaat ggttgttttc taattgataa tacctcacca cccatcactg
     8041 taccttccct cgtatcatca gcacctcccg atgcgtcacg tgtattcaag tcaacatgta
     8101 gcccatgtac atctccaata taggcatctg gtttatgttc cattgcatgt ccacgtgcaa
     8161 tcaatgctaa tattgtagat tgtgaaaatt gaggctccca ttcaatgcga ttaggatggc
     8221 tactataaat tctaggttca tctatagcct gctgaatatc catgccaaac actaatacat
     8281 tgattaatgt ttgcgcaaca ctagcaatga tacttatggc accaggtgca cctactgtta
     8341 atattagctt cccgtgatgc atcacaatcg ttggagccat gttacttagt ggtcgtttat
     8401 aaggtgcaat ttcattgatc ccacccgcca caacatcaaa accatccatt gtggtattca
     8461 ataacacgcc gtaccctgga atcgtaatac ccgatccgta aatcataccg attgatgtcg
     8521 taaatgaagc aatattacct tccttatcaa tcacacaaaa atgagttgtt tcggtatgat
     8581 tttcttcaac atctgtatga ctaatgcaat cagacacaac accatgctct atatcaatat
     8641 ttgctttatt gctatcaatg agcgtactgc gtgcttttaa ataatcgtca tcaattaatg
     8701 actgtacagg cagttcatga aaattatcat ctgccaagta ttgcgcacga tcactatatg
     8761 ctaaatgcat cgcctgtatc aaatgatgta agtaatcaac agatcttgga cccatagatg
     8821 gtaaatcgat gtgttctaat aacttcaata tttgaatgac agtgatacca ccggaactag
     8881 atggacccat tgaataaatg tcatagtctt taaatgtcgc actgattggc gctttaatct
     8941 gaatgtcata attggctaga tcctctaaag tgattgtccc accacattct ttgacaacat
     9001 taactaattg tttcgcaatg tcacctttat aaaatgcatt aaacccttgt tctcttaata
     9061 tttgaaatgt cttgcctaat tcgggttgta taatccaatc accttcacgc caatattgat
     9121 tttcatgcgt aaatacttgt gccgtttcgt ggtactttgt caatcgtgcg tgttgctggc
     9181 gcgaatattt ttcagtagcc caattggcag aatgaccttc aatagctagt tcaattgcag
     9241 gattaattaa atcttccaat gacaacttag cataatgctt gtgaatataa tcaaacagct
     9301 ttggaattgc tggcacagcg acagttttac catgtgtagt catatcaaaa aatgatttat
     9361 attcgcctga atcatctaga taaaattgtt tgtctacatg tgcaggggct gtctcacgcg
     9421 catcaaacgc agttatactg ccagtacttt gctcataata tagcaagtac ccgccgccac
     9481 caatacctga tgcaaatggt tctaccacat tcaatgtcag ttgaattgca atcactgcat
     9541 ccatggcgtt gccaccttga tctaatacat ccttaccaat tttagccgca agaggatgtg
     9601 atacggaaat taacccttct ttagatgttt ttgtctgttt gtcatttaag ttaatgacca
     9661 tactatatcc tcctactttc tgttaaatat ttaaaacatt attgattaat ggctttttct
     9721 actttttcta aatcttgacg ttgctcgtta ccagtatcga caagtggtgt aatcggtgat
     9781 gcaattttaa atttatcgcc acgataaaac ttaataaatt gatcctgatc tatcgcatta
     9841 actactgctt gtctcaagtt tggatgcgtc ttaaatatac cttttttaat atttagcatt
     9901 aaaaagactg acttgcgtcc atttttgcga ataatgctta aatttttatc cgacttaatt
     9961 aaatcaaaat gtttttgatt cacatctgcc aacatatcaa ttgaatgatt tctaagttct
    10021 gacaatgcat tattcggatc accattaaac ttcaatgtaa tatttttaat tttagctggt
    10081 ccataactac ctttttctgt ttcgttgaat cctggattac gttgaaacgt tgcttgatat
    10141 gcatttttct gtgtcataat gtatgcgcca cttgcataaa gcgcattttt tccatctgaa
    10201 tttgcaggaa ttgtactgct atccccatat ccttttggat attcttgatt tacttgatta
    10261 acaaattttt tagataaaat gcctgccgaa gagtgtgtta agtaatttac ctctcgaggc
    10321 atcgattgat ctgtcgtaat tttaacaatt tgataaatac cgtctttatt atttactttt
    10381 tgaccatctg tcgttaacga tttgacgtta tacgctttaa tcaacttatc atagatagat
    10441 ttatcgtcct tgtctttctc tttacgcaac tgatcgatgt cctcatcttt taatatcttg
    10501 atgtcattta tatgtttgtg catattgtaa gtattattat taggcacaga ctttttatca
    10561 cgtgctctat ctaaagaaaa cttaacatct tcagccgata cacgctctcc agtattacgt
    10621 gcttgtccat tgaccacttt cgtaaaataa tcatcatctc ttaacaagaa ataaaacgct
    10681 ttattgtcct tattcacagc ataatcacga cttaacgaac ctttcgttgt taagtggtca
    10741 ttttcatcta ataataataa ccttgtgtac atattcatat taattgaata tactgacggc
    10801 gcaattgaac gtattggatc taatgtagga atttcaccat cttgttgtgt catcacaagt
    10861 ggccgcgtat ctcgttctct actattgttg taatcaaatt gttgccatat taatgcacgt
    10921 gaatttggca atccaacact atttttatct aacactttat tgtcatatac taaattcttt
    10981 tttgatccat ataaaggcgc catataccct ttatcaaata caacttcatc ttcaatttgc
    11041 ttatatgttt gtttaacatc tgcttcattt tgagtagaag ctttatttaa caactggtct
    11101 acatgtttat ctttcaataa actatttgat cctgtagaac taaataatgc cgtcatagca
    11161 tagttcgggt caccgaatac tgtcatccag tcatcgattt ggatatcata attgccggct
    11221 tgacgttgtg tacggtagct accataatct ggttggatat tcatcttcac gttaaatcct
    11281 gcattttcca attgatcttt gacgatattc atatcatttt cataacttgc ctgtcctagg
    11341 aaatgtatcg ttggtcgctc gcctttcact tcaactttcg atgacttttg agccacttct
    11401 gatttcgtag ggataccaca accacttaat accaacgcta aaactataat tgcgatacta
    11461 atgatttttt tcacatctat ccctaccttt ttaatgaatt cttggatcta gtgcatcacg
    11521 cactgcatca cctataaaat taaatgctaa aacgacgaac ataatacaaa taccaggtac
    11581 aatagctaaa ttactgtgcg tttccaagta gttactaccg gtacgtaaaa tgttgcccca
    11641 ttcagctaca tcaggtgcaa caccaagtcc taggaaactt aaactacttg ttgttaatac
    11701 aaccacacct atatttaatg aaaaacgtac aatcataggc gcaatcgcat tcggcaaaat
    11761 ataacgccat atgatattcc aagtgttttc accagtgata cgtgctgcat ctacatattc
    11821 catgcgttta atttctaaaa cactggcacg cattgtccgt gcaaatgatg gtatattacc
    11881 gatacttaaa gcaataatta aatttggaat acttgctcca aatgatgcaa taattgccac
    11941 cgctaacaat aatgatggta ttgcaaacac tacatctaaa attcgcatta ttaaattatc
    12001 aatatgatta aaataacctg cgatagtgcc tagtaacaca ccaaaaacaa ctgcaataac
    12061 tactgaaata attgaaattg aaaatgtcag tttcgttccg acaactacgc gtgtaaataa
    12121 gtctctaccg aaatcatcag taccaaacgg ataggctaaa ctcggtccat gtaacagtgc
    12181 attgaactga tttttagtag ccaatgtcgt atcaaatgta aattgtgaca caattgataa
    12241 tgtcagcatg tagactaaaa taagtaaccc gataattgca atacgatgtc tagtagtttt
    12301 tcgtataaac gattcccatc cgttataact atgtatttgc gatgtacgtt ggtaacgtct
    12361 aatacttaca aacattaata atgtaaatac gttgcctgtt aatgtcatca acaataacaa
    12421 cacttcgacg atacgtcgcc ataggtcatg atgcttccat gtttgttccg ttgttaagat
    12481 gataattaaa atgatggtta aaacgattag caatgtttca gcaatataga acgtatcagc
    12541 cacataacct ttaaaaagat ttaatgcact cgttaatata actaaaatat aagttgcaat
    12601 agcataactt gcgaatagtt ttaaggaagc tatctttgtt gtaagtttta ccatatgcct
    12661 cacttccttt cgttaatttc actacgtaat tttggatcga ttaaagcata aaatatatca
    12721 ataattaagt ttgctaaaga tattacaatt gatatatata cgaccccacc catgactgct
    12781 gggatatcag gtattagttg tttttggacg atataacgac cgataccatt aatgttaaat
    12841 acttgttcag tcactgctga accgcctagt aactctgcca ctagaagacc aactagcgtt
    12901 acaattggaa taatggcatt tttcaaaata tgtttaataa ctacttttgt cgtcgataat
    12961 ccctttgcat aagcagttaa aacataatcg ctgcgcatta cttcaagtac agaagacctt
    13021 gtcatacgcg tgatagaagc agcaatactt gttccaatga caagtacagg taaaatcaac
    13081 gatattggat gttctggcat ataagatggt ggcaaaatat ccaatttcaa tgagaacgct
    13141 aaaatgaata ataacccttg ccagaaactt ggaatagaca aaccaattaa tgcaattatc
    13201 attaacgtga tatcaagcca actatttcgc ttcatcgcac tgataatacc aattggtatt
    13261 gcaataatta atgccacaat tagcgctaat actgcgacaa ttattgtaat tggaattctt
    13321 tcgccaactg ctttagtcac aacctcattc cctttgtaag tcgtacctaa gtcaaaggta
    13381 aaaacaccct tgatggtatc ccacaattga ataaaataag gttcgttaag atgatgtaat
    13441 acattgaatt gatgtatctg tgcctttgtt gcattttgtc ccagtatgct ataagccgca
    13501 tcaagcggtg aaaaatacag aatggtaaac acactgacaa taacaccaat gatgacaatc
    13561 acagccatga caattcgctc aaaaatatat ctaactagtg gctgtaaata aatagtcaat
    13621 aagatgaaca tcggcaaggc caatatcact ttgatcatga tgaacttatg aaataataca
    13681 ttttcaaagt atgttgaaaa atgtgcttgt tcaatattct ttgaattcgt attagaactt
    13741 tgtgccttga atatttttaa tgcttcttta tgtatttgtg tggatgactt ttgctgcgat
    13801 aaatatttat atttttgatg taacgcctgt tcaatttctg aaatttcaga attattagcg
    13861 taaaaatttt ttctcttagc agaaaagaaa aactttatca ctgcatataa aaatattggc
    13921 aagcttaata ccgataatac aaacttgtaa aatgccttgt gatacaacga cttaaaagat
    13981 tctaaataag taattatgct aatcactcct tgtaaaataa tcttgagtag attactatga
    14041 tatacaaaag tatagaataa atttacacat ttgtgaatag ggaggcacaa catcatgtca
    14101 aatttattag aagtcaacag tctgaatgta caattcaatt atgaagaaac tacagttcaa
    14161 gcggtaaaaa acgtctcttt cgaattacga aaaaaacata tcctaggtat tgttggtgaa
    14221 tcaggatcag gaaaaagtat taccgctaaa tctattttag ggctactacc agattatccg
    14281 gatcacacgt taacaggaga aattattttt aatgggcaat cgttaacaaa tttatcaact
    14341 tcagcgttac aacaaattcg aggtaaggat atttcaatga tttttcaaga tccactctct
    14401 tcgttgaatc caagattaac gattggcaaa caaattacag aagtactatt tcaacataaa
    14461 cgtgtatcta aatctgaagc aaagtcgatg acaatagaca ttttagaaaa agtaggtata
    14521 aaacatgcaa ctcgacaatt tgatgcttat ccacatgaac tttctggtgg tatgcgtcaa
    14581 cgtgtcatga tagcaatggc attgatttta aagccacaaa ttttaatcgc agatgaacca
    14641 acaacggcat tagatgccag tacacaaaat caattactgc agttaatgaa atccctttat
    14701 gagtacacag aaacgtctat tatttttatc actcacgatt taggcgctgt gtatcaattt
    14761 tgcgacgatg tgattgtaat gaaagatgga agtgtcgttg aaagtggcac ggttgaaagt
    14821 atttttaaat cgccacaaca tacctatacg aaacgcttaa tagatgcgat tcctgatatt
    14881 catcaaacgc gtccgccaag accgttaaac aatgatattt tattaaaatt cgatcgcgtg
    14941 agcgtggatt acaagtcacc aagtggtagc ctataccgag cagttaatga tattaacttg
    15001 gctattagaa aaggcgaaac attaggcatt gtcggtgaat cagggtcagg gaaatccaca
    15061 ttagctaaga cggtcgtcgg tctaaaggaa gtgtcagaag gctttatttg gtataacgaa
    15121 ttgccattaa gtttatttaa agatgatgaa ttgaaagctt tacgacgaga gatacaaatg
    15181 attttccaag atccattcgc atctattaac ccaagattta aagtcattga tgtgattaaa
    15241 cgaccactta tcattcacgg gaaagtcaaa gataatgacg acattattaa aactgtcgta
    15301 tcgttgttag aaaaagttgg cctagatcaa agtttcttat atcgctatcc acacgaatta
    15361 tctggtgggc aacgccagcg tgtaagtatc gcaagagcac ttgctgtaga acctaaagtg
    15421 attgtttgcg acgaagcagt gtcagcttta gacgtttcaa ttcaaaaaga tatcatcgag
    15481 ttattaaaac aattacagtt agacttcggc atcacttatt tattcatcac acatgacatg
    15541 ggtgttatca atgaaatatg tgatcgagtt gcagttatga aaaatggcga aatcgttgaa
    15601 cttaataaca cagaagatat tatcaaacat ccgcaatcag actatgcaaa gcaacttatt
    15661 tcagaagtag cagttattgc taaataaaag tcatgcgttg tgcaacttta tcactgtatg
    15721 gtctggaata aatcgggcaa catctgatgt gttatgttag atgactttgt catactaact
    15781 atattgtgct atgttagggc tcggcaccat atcaagtttt aggtgccttg ccattattta
    15841 agggtcacac ccatactctg ggcattttaa cgccagaata aatcccccgc cactatgtga
    15901 agtgcggggg atcatttgca tccttaagaa aatgatatag tttattttta tctttctttg
    15961 tcatttttgc gaaatataac aagaacttaa tttcagtcgg agctgctaaa aattcatctt
    16021 tgtattcttt catgttgcct ctttttttca actcagaaat catgttgtta tgcagtttcg
    16081 tcattggtat ttctttaaac acgtcaaata acacatcata ctctgtgaaa tcattataag
    16141 ttggcacatc catatgattt tgtgcaataa ctctttttct ttcttctttt cgtaaaaagt
    16201 tatacatcgc ttttgagttt aagagactat tgttttcttt tggtttttga acttcactca
    16261 gtgtatttct agatgttaat actaaaattc cgactgttgt atctttgtat ctagccataa
    16321 ctttattcaa gtgtttgtca tttgtaatga ctacgacata attaaacact tcataataat
    16381 cattaatttg attatctaat ctatccagct tatctaattc tgttttaatc tcatagacaa
    16441 cacctttgcc gtttaacaat ataaaatcag caatactttt ccctatgggc atctcagaaa
    16501 gtgcagtagt tgtattaaca gaatgtcgtc ctagaaggag tttattaagc attgtgtttt
    16561 tgtaaaaata ttcgttacga taagactttt taagatagtt atataaagac ttcattttct
    16621 gtattacatt gatattttta ggcccttgta tataaaaatc atatgcctca tcaacaacat
    16681 cacttttgtt tttgattgca atttgatgta atatcttttg actaaaaaca cgattaatca
    16741 tatttctatt atccatagct acgcacctcc ttcaacatac atttaatact tatcctgtta
    16801 atattataaa ctattttaaa tgcctataga cattatttct atacacatac gatgataaaa
    16861 ttttattgaa accaaatgat gattgccaaa caaatgagcc ttttctaatg aaataataat
    16921 gccccctaca ctaatctagc gagggggctg aatcattaca caccgtattt ttcagttgtt
    16981 tctgccacga aagattcaat ggcttttctt gctttacgct tttctttcaa tggcaaatca
    17041 ccaatcattt ttttaaggtg atgtgggttt acaacaccac tatactggta gtcatttgaa
    17101 attgttttta gggcttgttc atcgatagat ctctctccag caaatccttt gaactccgct
    17161 tcttttttaa tactttcgaa attaacatat tcttgatcga tatcatcatc cttatttaaa
    17221 gaaggtacaa cattgtcgat gaattctcta attagatctc gttttaacct caatgtcgga
    17281 tcatctgcat gatctaaaat gcgtctaatt tgttcttggt tacgacgttg ttccgctttg
    17341 tcttcaagat caatttgtct caatatattc ataatataat tcacattaat cgtatcatta
    17401 cgcatcattt ctatttcgaa atcaatatca tttaaaatgg atactttatt tttctcagcc
    17461 gtcgctcttt ttacttgatc gtacacagct aaatatttac ttttatagtc ttcattctct
    17521 tgttcatcca ttccaatttc atcaattgta aactcaaact cgtcaaatgc ttttaaacgt
    17581 aatattattt tagctaataa acgataagct tcaacaaagc gctttagctc ttcttcatct
    17641 tgaatgtcat caaccatatg tggtgtcggc acaatcattt taagctcacg ataagcgtcc
    17701 ataaattctt ttttatactc ttcataacta cgcattaaaa ttgtatccgt atcatttgtt
    17761 tgtgagaata ctctcagtgc atcgtctgtc tcttttttca agtcacgata gtttacaatt
    17821 ttaccaaatg gctttgattc tttttcaacc ctatttgtac gtgaatacgc ttgaattaaa
    17881 tcatgataca ttaaattctt gtcaacatat aaagtgttca gtactttact atcaaaacca
    17941 gttaagaaca tattaacaac gattaagata tcaattttac tatctttaac gcccttttta
    18001 acgttttttg aaatatgatt aaaatactca ttagttgtgt ctgttgaaaa attcgtctcg
    18061 aactttttat tataatcgct aatcattatc tctaattttt cacgtgaatg atatggcact
    18121 tcaccatcac gatcatcttc attaggttta aacgtaaata taccagctac cgttaatggt
    18181 tgttccaact ttttgttaag tcgcttaaat gtctcataat atttaataag cgcgtgaata
    18241 ctttggactg taaatatact tgaatattga cgattacgtg tatatttatc atgattattg
    18301 atgatatgtc gtgttactaa ttccacacgt ttatccgcta accatacttc ttccgtatca
    18361 attgcttcaa ccatgctgtt atcttctgct tttaaagctt tatttttaaa agtattaata
    18421 tagtcaactg agaaaccaag tacattacca tcatgaatgg catctctaat taaatacgta
    18481 tgtaagcatc taccgaaaat atctgcagtt gtcctaccat cttgactact attttctgga
    18541 aaacgtggcg taccagtgaa tccaaagtat tgggcatttt tgaaatgttg tttaactaga
    18601 cgatgcatgt caccaaattg actgcgatga cactcatcaa taataaatac aactttattc
    18661 gttttatact gttctaataa atgggcattc ccttgaatcg ctttagccat tttttgaatc
    18721 gtcgttacaa taagtggcaa acttttatca tttagttggc gtaccagttg cgaggtatta
    18781 aaagttttgt ctacagcacc cttagcaaat ttattaaatt cctcttctgt ttgactatcc
    18841 aagtctttac ggtcaaccaa aaagataact ttcttaatgt catcttgttg tgataaaatc
    18901 tgactcgctt taaaagaagt caatgtctta ccacttccag ttgtatgcca cacatatcca
    18961 ttattccctg tctcagtcgc ttgttgaatt agtgcttcta ccgcatacac ttgatacggg
    19021 cgcattgcca tcagtattct atctgtttca ttaataatca tatagcgcga tatcatctta
    19081 gctaattgac aaggtctcat aaacgactca gcaaatgatt gtaatgtatt gattcggtta
    19141 ttctgtttat cactccaata aaacatgtga ctcttcaata gttcgctatc attattagaa
    19201 aagtatcgcg tttcaacacc attactaatg ataaacattt gtatgtagcg gaataagcct
    19261 gtgtaatttt gtttgcggta acgttttact tggttaaacg cctcattaat atcaatacct
    19321 cgacgtttca attcaacttg gacaaggggt agtccgttga ttaatatcgt tacatcataa
    19381 cgtgctttat atgtatcctc gacagatact tgattcgtca cttgaaactt atttttacac
    19441 caacttttcg tatctaaaaa cgacaaataa atctcagact catcatcacg tctaagtggt
    19501 aatttatcac gtaaaatacg ggcactctcg aaaatacttt tcccatcaat catcgttaac
    19561 agacgttgaa actctttatc tgttaaggga ttgccttcta atttgtccgc atgacgctca
    19621 tttaaaatcg ttctaaaatt atcaagcaat tgcttattat cacgtatcgt tactctttcg
    19681 taacccaatt gttcaagttg attcatcatt tcattttcta atgcgtattc actttggtat
    19741 gccattcata tccccttcca tacactttct attgctctaa atatatcata aactttaatg
    19801 aaaaatgttt gttttttatc ttcaaacgta aatttattct aattttattc tctgtatcat
    19861 ttaatatttg tgtttgaggt aagtcgtata ctaaaaacac caacacacta ttctttatat
    19921 ttttatactt ctatgccaga gttcaacttt tgaatatatt gtgtcctcaa ttgtttgttc
    19981 tctgtatata ttttattgaa tatgattgat tgaaaaacaa aagtgaaaat gtatatcaca
    20041 attggaataa aagcattata aatgccatta tcaaaatagg ataaagaaaa aataaataaa
    20101 gtgaacacta tcggcattgt aaaacgacat aaaataatag accaattatt tataaaaagc
    20161 caacttctta ttaagttttt tctattcagc gtaatgatat ggaatatttt gtcgttttta
    20221 ttttgataaa aactttcaac tttttcatag tgtccatata tggtttgatt taggttaatt
    20281 attttgtagt ttgatccagg ttcgtaaagg tcattattgc caactttaaa acaatcttta
    20341 attaatgtac tttctttgtt tacaacaaac ctataggaat gtatgtaatc acaattttca
    20401 cacattttat ataattgaaa tctattaaca atcgctaaat aatttctgta agttgaaata
    20461 gctataaata agattaatcc ataacttatg cctattaatg aaaaccatat tgtattaata
    20521 aattcactat gaggtctttc tagcagttta atatatattt tataattca
//
LOCUS       DAPIDM010000034        20550 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000034 DAPIDM010000000
VERSION     DAPIDM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.034"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..270
                     /locus_tag="RJE42_001109"
     CDS             <1..270
                     /locus_tag="RJE42_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDV5876540.1"
                     /translation="LFRQQKRLNDIILCENHSPILTEQPTYFNEYDLRLQDVCALLKH
                     RERQWLMLYLEGYKQYEIADIMSLSTSTIKLIKASVKRKCQRFLN"
     gene            383..826
                     /locus_tag="RJE42_001110"
     CDS             383..826
                     /locus_tag="RJE42_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDV5876541.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINAKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTNDH"
     gene            complement(813..1256)
                     /locus_tag="RJE42_001111"
     CDS             complement(813..1256)
                     /locus_tag="RJE42_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876542.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSITDKDNVNFNKPI
                     TFANTYLRENVFRSKESKSYSIFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVNPK
                     VDLSTTVVILTIIGAILLMLVSIYLIKITTSYFTKKLLEHKFNGH"
     gene            1554..2189
                     /locus_tag="RJE42_001112"
     CDS             1554..2189
                     /locus_tag="RJE42_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDV5876543.1"
                     /translation="MQKFKDLFYDDLSVARGNYFLTLLSAFLITIILLIGFVVSDTHI
                     LYGVFIVLIGIVLLRLFKINLLSFKKLTMSQVIYIIGGALLIYGLDYLYLHFHDVPTN
                     EQELDRELQHMPLYMSIVTIAIIPAIVEEIVFRGMIIRVVFRKHLFIGLVVSSLVFAS
                     LHESDTWIGYLPYLYSGVIFGLIYLKTKRLEVVILIHFINNLSSLLILLWG"
     gene            2356..2976
                     /locus_tag="RJE42_001113"
     CDS             2356..2976
                     /locus_tag="RJE42_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDV5876544.1"
                     /translation="MFSRNNNIFVDDLSVTKDNYLIAVLKMLVIFLVINYGTDVSYDH
                     EYVGLFIILLGVVLLKILNINLLSFKKLKFTHMLYIIFGFLLMYGLDYLYDTFAPPTL
                     NEILIDEESEDAPYHIALLSTAIIPPITEEIICRGLIIRILFRNHLFLGFIVSTVFFT
                     LIHESDTLIGYLPYFYSGLIFGYTYLKTKRLEVPILIHFINNLLAM"
     gene            complement(3539..4252)
                     /locus_tag="RJE42_001114"
     CDS             complement(3539..4252)
                     /locus_tag="RJE42_001114"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV5876545.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            4511..4813
                     /locus_tag="RJE42_001115"
     CDS             4511..4813
                     /locus_tag="RJE42_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876546.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIILILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVHTFGFI"
     gene            5068..5433
                     /gene="crcB"
                     /locus_tag="RJE42_001116"
     CDS             5068..5433
                     /gene="crcB"
                     /locus_tag="RJE42_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV5876547.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIEFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDYQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            5430..5783
                     /locus_tag="RJE42_001117"
     CDS             5430..5783
                     /locus_tag="RJE42_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDV5876548.1"
                     /translation="MISIILVMIGGGLGAIARSAVTDYFNHIFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFDIKLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(6033..6866)
                     /locus_tag="RJE42_001118"
     CDS             complement(6033..6866)
                     /locus_tag="RJE42_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV5876549.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAIMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFRIFDFELSDEQMTRIDDLNQDKRIGPDPKTFE
                     G"
     gene            complement(7078..7986)
                     /locus_tag="RJE42_001119"
     CDS             complement(7078..7986)
                     /locus_tag="RJE42_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDV5876550.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMISTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKHLGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(8111..9304)
                     /locus_tag="RJE42_001120"
     CDS             complement(8111..9304)
                     /locus_tag="RJE42_001120"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV5876551.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(9410..9521)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            9676..11268
                     /gene="pckA"
                     /locus_tag="RJE42_001121"
     CDS             9676..11268
                     /gene="pckA"
                     /locus_tag="RJE42_001121"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV5876552.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVSEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAKYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(11565..12311)
                     /locus_tag="RJE42_001122"
     CDS             complement(11565..12311)
                     /locus_tag="RJE42_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDV5876553.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVKRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     QAFIHMVGFSRGGLQGLLTFQDLPVSSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRSAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGAAHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHR"
     gene            complement(12316..12789)
                     /gene="ytkD"
                     /locus_tag="RJE42_001123"
     CDS             complement(12316..12789)
                     /gene="ytkD"
                     /locus_tag="RJE42_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDV5876554.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            12855..13112
                     /gene="yidD"
                     /locus_tag="RJE42_001124"
     CDS             12855..13112
                     /gene="yidD"
                     /locus_tag="RJE42_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV5876555.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(13109..14110)
                     /gene="menC"
                     /locus_tag="RJE42_001125"
     CDS             complement(13109..14110)
                     /gene="menC"
                     /locus_tag="RJE42_001125"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV5876556.1"
                     /translation="MKLTAVHFYKYSEPFKSKIVTPKVTLTHRDCLFIEIIDDKGNAY
                     FGECNAFQTDWYDFETIDSVKEALQQWFIDIKNKSFESYEAALELLSPLEDTPAARAT
                     VVMALYQMFHTLPSFSVAYGATVSGLSNKQLAALKETKPARIKLKWTPQIIDQIKSLR
                     ELNFQFQLVIDANESLTRQDMTQLQLLAHENILYIEEPFKEISMIDEVVAEKTPPIAL
                     DEKATSLSAIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(14115..15593)
                     /gene="menE"
                     /locus_tag="RJE42_001126"
     CDS             complement(14115..15593)
                     /gene="menE"
                     /locus_tag="RJE42_001126"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV5876557.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKTY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITMNDLMDNTLGIQYDTSNETVVPKGSPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYDLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHERPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTDTFENGYFNTGDIAEIDHAGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISE
                     VQLIAYLSQHLAKYKVPKYFEKVDTLPYTSTGKLQRNKLNRG"
     gene            complement(15752..16207)
                     /locus_tag="RJE42_001127"
     CDS             complement(15752..16207)
                     /locus_tag="RJE42_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDV5876558.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDELFHLSALQDMNHRPIDIADDVATMDKSPHQNDRMTLKLNKNN"
     gene            16510..17172
                     /locus_tag="RJE42_001128"
     CDS             16510..17172
                     /locus_tag="RJE42_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDV5876559.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSAKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNESSKTQLTQKDEDKSENMPEEKSESETDKDLQSTE
                     EVPANENTENNQHEIDEITTTDQSDDEINTPNVAEDKSQDDLKDDLKDDLKEKQQPSD
                     HHQSTQPKTSPSTETNKQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPD
                     KY"
     gene            17253..18248
                     /locus_tag="RJE42_001129"
     CDS             17253..18248
                     /locus_tag="RJE42_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDV5876560.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQHQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGVTGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QNDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMRQSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            18324..18950
                     /locus_tag="RJE42_001130"
     CDS             18324..18950
                     /locus_tag="RJE42_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876561.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNREKQMTPQEAEDIVRNDYKARGVNEYQTLNFKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDQAETDNESEDI
                     QHHDIDNNKAIQNDTPNQKVDDKNDKNADNKEEKHDNRENNSAETKVK"
     gene            18991..19335
                     /locus_tag="RJE42_001131"
     CDS             18991..19335
                     /locus_tag="RJE42_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDV5876562.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSSLGVKRDIINLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIMQEK"
     gene            19433..20005
                     /locus_tag="RJE42_001132"
     CDS             19433..20005
                     /locus_tag="RJE42_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876563.1"
                     /translation="MEKNEYTAKYNEYSQLLDVTYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLKGIEIYDFLESR"
     gene            complement(20203..>20550)
                     /locus_tag="RJE42_001133"
     CDS             complement(20203..>20550)
                     /locus_tag="RJE42_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDV5876564.1"
                     /translation="TIYINNNLSINNTRFAIARELGRYLYKIKDNNDNSLKNLHEMIY
                     ESDVNQFSVDLLMPKKQIEALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKV
                     AAGLRLYNLGIHI"
BASE COUNT         6875 a         3626 c         3072 g         6977 t
ORIGIN      
        1 ctattcagac aacaaaaacg tttaaatgat atcattttat gtgaaaatca ttcaccaata
       61 cttactgaac agccaacata ctttaatgaa tatgaccttc gtttacaaga tgtttgtgcc
      121 ctactaaaac acagggaacg tcagtggctt atgctttatc ttgaaggcta taagcaatat
      181 gaaattgctg atattatgtc attatcaact tcaacaatta aattaattaa ggcatcagtt
      241 aagcgtaaat gccaacgttt tttaaattag tttattgttt aatttcggtc gatgttttta
      301 cgatgttgaa acaccctaac atcttcatat aaatttattt taaccattaa attaacaact
      361 tattatacga ggagtactat atttgcaaga caattctaca aaatacctac tttatatcca
      421 aactgctact tcaaaccatc tcgaaacaaa ctgtgtcttt ttacattgcg attacattct
      481 taaagttcca attaacaaac tcgtttcata ttatgccaaa ttgcatttat catcacaaag
      541 tgtgctaatt gagactgcaa aaaacatact aaatattaat aaactggttc ccatttacat
      601 caatgcaaaa actatacttt ttccgttaaa acataaacgt gcaccgatac aaatttatat
      661 caatgcacat tatattgttg gtatgactgc catagagaat tcaacattaa tacattttca
      721 agaaggcatt cagctagaag ttgatgagcc attttctcta gtttcaaaaa aatgtcatga
      781 aagtttagca ttgaagcatt tcattgaaaa tactaatgac cattaaattt atgttctaac
      841 agtttctttg taaaatatga tgttgtgatt tttattaaat agatactaac taacattaat
      901 aaaatcgctc caatgattgt taaaataaca accgttgtcg acaaatctac tttagggtta
      961 acgaaaaatt gcacaccaga tagacttgta attaatccaa ataaaaacaa acctaataaa
     1021 gcaacgtatg caaacatcat cactactaat gttacaaaga tgctataact tttagattct
     1081 ttactacgaa atacattttc tcttaagtac gtattagcaa aggtgattgg cttattaaag
     1141 ttcacattgt ccttgtcagt aatactagac aaccgttgat ctaatgcctg tttttccttt
     1201 ttgttcaaaa accataattg ctttttaacc tttttatcga acgatgcttt attcataatt
     1261 aaggatttct ctcctttttc atattgacgc tattttatac atgattgcca atgaacattg
     1321 tattttatat attttacaga aatacatgca tcaaatctac aaaaattaac tttgcaattt
     1381 tatttttagt aatttgacac cgtataatta acaccgttta tattgcaaac aaaaggtttt
     1441 agttatgtgt aatgatgcgc ctttaatcat atctaagttt ctcatataat gaaaatgacc
     1501 ataccaatga aatgctttaa tatttatttt aaagatgata gaaaggatta aatatgcaga
     1561 aattcaagga tttgttttat gatgatttat cagttgcacg tggaaattat tttctaactt
     1621 tattatccgc attcttgatt actattattc tactaattgg tttcgttgtc agtgatacac
     1681 atatacttta tggcgtgttt atagtgctca tcggtatcgt gttattacgg ttattcaaaa
     1741 ttaatttact ctcatttaaa aaattaacaa tgtcacaagt gatttatatc atcggtggtg
     1801 cattgctaat ttatgggtta gattatcttt atttacattt tcatgacgta ccgacaaatg
     1861 aacaagaatt agatcgagaa ttacaacata tgcctttgta tatgtctatt gtcacaattg
     1921 ctattatccc tgccattgta gaagaaatcg tttttcgagg tatgataata agagttgtat
     1981 ttagaaagca tttatttatt ggattagttg tgtctagtct agtgtttgca tcattacatg
     2041 aatcggatac ttggattggg tacttacctt atttatattc tggagtgatt tttggcctaa
     2101 tatatttaaa aacaaagcga ttagaagtcg ttattttaat ccactttata aataacctat
     2161 cctcattact cattctgcta tggggataat actagttgat ataatacaca atgattgccc
     2221 ctcaacatgt tattcacaaa acgatatgca atacaaatta ataatataca agcttttaaa
     2281 acaataaaaa accttgtata attaacttaa ttttcataaa aattaaacgt caaactttat
     2341 tttaggagaa ttaatatgtt ttcgaggaat aacaatattt ttgttgatga tttgtctgta
     2401 acaaaggaca attatttaat tgcagtttta aaaatgttgg tcattttttt agtaattaat
     2461 tatggaacag acgtcagtta tgaccatgag tatgttgggc tatttattat attactaggt
     2521 gttgtacttc ttaaaatttt aaatatcaat ttattatctt tcaaaaaatt aaaattcact
     2581 catatgcttt atattatatt tggattttta ttgatgtatg gattagatta cttatatgat
     2641 acgttcgcac cgcctacatt aaatgaaata ttgatagatg aagagtctga agatgcacct
     2701 taccacatag ctctgcttag tactgctatc attccaccaa ttacagaaga aatcatctgt
     2761 cgtggattga ttatacgaat tttattcaga aatcatcttt tcttaggttt tattgtttca
     2821 actgtattct tcacgctaat acatgaatca gacacgctga ttggttatct tccgtatttt
     2881 tattcaggtt taatttttgg ctatacttac ttaaaaacaa aacgattaga ggtaccgatt
     2941 ttaatacatt ttatcaataa cctcttagcc atgtaacaat aaaatcatta attattttga
     3001 tatttacatg atgacaccaa agcttttaca aagtattaat aggtgacttt ccaaatgtta
     3061 ttgcatattt acgttctgta attatatcaa aacaacaaaa catattaagt ttagctatga
     3121 aaaaaatcta tgacgatagt ttttttcata gctatttttt atagttattg agcctatccc
     3181 agactctcta tactggtata ttagcaatca ttgactgaat gataatacgc ttttcaatta
     3241 taggggcccc aacacagaga atttcgacaa gaaattctac aagcaatgcg cgttggggtg
     3301 tggggcccca acatagagaa tttcaaaaaa gaaattctac aggcaatgcg cgttggggtg
     3361 tggggcctca acataggaaa gatcagctca cattaatgtg caagatggcg gtgcccaaca
     3421 tagagaaatt gtatttcaat ttctacaggc aatgaaatac atattaattt aaaattgact
     3481 ccaatatgaa aaacccccat aagcacaatg cctatgaggg tttaaatata tttatatatt
     3541 ataaaataga aagacctgaa gattgaatat ctttcgcaaa gcctttaact gtatctactg
     3601 ataattcgtt aatatcgcga cctaagtttg tgttcacttt tttcacaaca tctgctgggc
     3661 atgtaataat atctgcacca atttcatcag cttgaatcac attgaataat tcgcggcaac
     3721 ttgcccataa taatttaacg ccgtctttac tatgcgtaac ttttacagcc tctttcatta
     3781 atggtaatgg atctacgcct gtatctgcaa tacgtcctgc aaatactgaa acatatgttg
     3841 gcacaccttc agttactgct tcagttattt ctttaacttg ttcaattgta taaacagctg
     3901 taacgtttaa tctcacattg tcagctgaaa gttttttaat taaaggaatc gttgattcac
     3961 cttttgtatt tacaatagga attttaacaa atacattttc gccatattgt tttaaaattg
     4021 ctgcttcttt ttccatagtt tctaaatcgt ctgcaaatac ttcaaatgaa attgaagcgt
     4081 ctggaatttc tttcacagct tcttcagcaa aagctttgta atctgttacg cccgctttcg
     4141 ccattaaact aggatttgtt gtaaaaccat ccacttgttt gtttttataa gctgctttca
     4201 tttcttcaat atctgcacca tccgcaaata cttctacatt tagtttagcc atataatata
     4261 gcctccttga ttcttattaa aattttaaca acatctgcat gtctttttct tacaaccatt
     4321 tgtaaaaaat gatttttatt tctttgttat ataaaacatt tatatcacgc ttttataata
     4381 tatagctaat tttttgccta tatttcttaa aaatgacttt agcaacattg tgtcaacaag
     4441 gtaattaaac tattgtttta tgattgttta tgatacgata tgtaaatgat aaccatctag
     4501 gaggtctagc atgtctcgtt caaaaaaata cttttactta tctagcttaa tgattatttt
     4561 aagctttttc tttaatacaa ataacgtttt cctaagtggg ctttttaatt cttttattaa
     4621 attaatactt ttctgcagtg ttattaactc aattatactg atattgtcta taatttttgc
     4681 agatcgttca attaaatcac taaagcctga tgcagattgg attagaattg cgagtaaaag
     4741 tttgccttgg attattctaa tcgttatttt agtacatatc ttttcgattg ttcacacatt
     4801 cggttttatt taaaaaagat aaaatgtcaa tgtagcctta atacaaaaca atacaatgta
     4861 tcatgctata ataagtaaat caatttgatg acgttgttgc atataaaaaa tattagattt
     4921 tcgaaatcat aactatgcat ctaatcgcta tagttataca acaagatata acatataatg
     4981 aggtttgata atgcatcgac aatttttgtc gtcgcgttgc caaaacctct tttttaattc
     5041 aaactacttc ttttcgaggt gaaccaaatg caatatgtat atatttttat cggtggtgct
     5101 ttaggcgctt tattacgtta cctcatttct tttctaaata ctgacggagg tttcccaatc
     5161 ggaacactga tagccaattt gactggtgcc tttgtaatgg gattgctaac agccttaaca
     5221 attgaatttt tttcaaacca tccaacccta aaaaaagcta ttacgactgg ttttcttggt
     5281 gctttaacga ctttttcaac atttcaatta gaattaatac atatgtttga ttatcaacaa
     5341 tttataactt tactactata tgctgtaaca agttatgtct ttggtatttt gttatgttat
     5401 gtcggtataa aactaggtgg tggtttatca tgatatcaat cattttagtc atgattggcg
     5461 gcggtttggg tgcaattgct agaagtgccg ttactgatta ttttaatcat atatttactt
     5521 ccaagttacc tatcgcaaca ttgatagtaa atcttgttgg tagtttttta attggattaa
     5581 ctataggctt atcaatttca atctcatggt tccctgcgtt ctttgttacc ggttttttag
     5641 gtggcttaac aactttctca acattagcca aggaacttac attaatgatg acaccaaaat
     5701 ttgatattaa gctttttctc aattattcac ttttacaatt catcattgga tttatagctt
     5761 gttatattgg ctatcatatt taaaaataaa atgcttcatt cagcaaatag gtaaattacg
     5821 acaccttcct gaacgaagca ttttttaatt ttcatgcaaa ttttttagca ccatataatg
     5881 cctatcaaaa taaaacaatc tttgttgccg tttaaataat gtgaatatca acaaattctc
     5941 caaactagtc gaaaataaaa cgttaagcaa tatgtctatt acctaacgtt gacaatttat
     6001 ctgaccatat caaaaattca ccgatttgtt tcctatcctt caaaagtttt tggatcaggt
     6061 ccaattctct tatcttgatt taaatcatca attcgcgtca tttgttcatc tgataattcg
     6121 aaatcaaata ttcggaagtt ttcagagatt ctgtttggtg tcaccgattt agggattgta
     6181 accacaccat gctgcacatt ccatcttaaa acaacttggg caggtgactt tcctaattct
     6241 tgagcaatgt ctttaattgt ctcatcattt aaaatttgtg cattcatcaa tggtgaccaa
     6301 gattccatca cgatatgttg tgctgccaaa tataatttca atttatgttg cgttaaatat
     6361 ggatgatatt caacctgatt aattacaggt tttattgaca cttgtgccaa caaagcttcc
     6421 aaatgttcag gttcaaaatt gctgacacct atatttttaa ctttattatt tttatataaa
     6481 tcttccatac ctttccatgt atcaaccatt atggcttcgt tcgtacctgg ccaatgtact
     6541 agatacaaat ctaagtattt taaacctaat ctagataaac tagcttcgta agcagctgct
     6601 acattttcac gaccaaaatc ctcaaaatat aattttgaag taataaataa gtcttctcta
     6661 gcaataccag ttgattccaa tccggcacga atgccagcac ctacttgttc ttcattccca
     6721 taaacttttg cggtatcaat actacgatat ccttgttcaa tggcatactt aacactttcc
     6781 atgcaatttt catcattttc cacacgaaat gtccctaaac caatttgtgg catcgtgttt
     6841 ccattataaa atgttttaac ctccataaat atcgcctcac ctttttgatg tattataccc
     6901 tgttatcata acaaatctga gttgaataca tgagaaaaaa cacttatagc aatcaaccac
     6961 taaaattcta gtaatatctc tcaaatatta atcaaattgt aaaagtaatt ctgtttaatt
     7021 tatgacaaac taaaaaagcc gaagtaacaa catatagtca tcacttcagc ctaacattta
     7081 attgaatgat tcaattttat ccatcatttg ttgtaagtct tccacattgt attgaatacg
     7141 accatggaat acaaatttgt taaagaactc gtctaattgt tcagcaccga caagcacttt
     7201 gacagcacta ttttgattat aatttgaaat cgttacatcg ccttcatttt taggattaaa
     7261 gtataaaatt gaagtcggtg tatatttagc acctaaatgt ttttgtaagt cttcagccaa
     7321 ttgtttaatc gcctcaattt gatctgaata atttacaaat gataatgaac gtttgtcatc
     7381 attttgatcc atcacaatag tttgcggtct agatttatct aaatccaatg tatcaaatac
     7441 ttgttccata ggtggtaaat ctttaaattg accgccactg ataccattat atacatgacc
     7501 tttcaacaat tgggaatcaa tgatataaag accagttctt gttaatacta aatgactaat
     7561 acgttcaata ttattaaagc catcctttgg taaaaagata tttgccataa tgtgcatatc
     7621 ttctggtcga attcgttttt ctttaactaa tctttcacga ataccaatta atctcatgtc
     7681 cgttacatat tcactatgat ttttcgagaa caattttaat gcgtcaatct cacgatcttt
     7741 tgtactaatc atgtgattat aatcttcttg ttgttttgta attgtctttt tattttgaat
     7801 acgctctttc tctaaagctt cttcatgaga ctttttaatg ttttgttctt gttgttcata
     7861 cttttcttct gtttgtcgct taactttttt cttactacct aaggcaacta agaaaaggac
     7921 aaaaaagatt aatgcaatga ctactgcaat aatgagtcca atgactaccg gtgaagataa
     7981 atccatcaca acaacgctcc tttttaatat atgaataact ttaattataa tagaaaagct
     8041 aaagattttc gatacatatt atcatttata taccgaaaat cttttattta gctatattca
     8101 attcatctta ttattttact gcgtctttta attcttccac tttgtctaat ttttcccatg
     8161 ggaataagac atctgtacgt ccaaagtgac cataagcagc agtttgtttg taaatcggtt
     8221 gtttcaaatc aagcatttta ataatacctg caggtcttag gtcaaagtgt tttctaactg
     8281 cttcgacaag ttgtccttca gaaactttac ctgttccaaa tgtatcaatt gcaattgaca
     8341 ctggttctgc aacaccaatc gcatatgcca attgtacttc acattgatct gctaaacctg
     8401 ctgctacaat atttttagcc acataacgtg cagcgtatgc agctgaacgg tctacttttg
     8461 taggatcttt accgctaaag catccgccac catgacgtgc atagccaccg tacgtatcaa
     8521 caatgatttt acgtcctgtt aatcctgcat caccttgagg tccaccgatt acaaagcgtc
     8581 ctgtaggatt gatgtagaat ttagtttgtt cattaatcaa gttttctgga acagttggat
     8641 aaatgacatg agctttaatg tcttcttgaa tttgttcaag tgtcacatcc tcagcatgtt
     8701 gtgttgatac gacaatcgta tcaatacgta cagggttatc attttcatca tattcaacag
     8761 tgacctgaac tttaccgtct ggtcgtaaat aatttaacgt gccatcttta cgcacatctg
     8821 ataaacgttt tgccaattga tgtgataaat aaattgctag aggcatatac gtttctgttt
     8881 cattcgttgc gtaaccgaac attaaacctt ggtcacctgc acctgttgct tcaatttctt
     8941 cttcgctatc tttatcacga tactctaatg ctttatccac accttgtgca atatcaggtg
     9001 attgttcatc aatcgcagtt aaaattgcca ttgtttcata atcatagcca tattttgctc
     9061 ttgtgtatcc aatttctttg attgtttctc taacaacttt cggaatatca acatatgttg
     9121 ttgtagaaat ttcgccggcg atcaatgcca tacctgttgt aacagttgtt tcacaagcta
     9181 cacgtgcatt tgggtcgtct tttaaaatag catctaatat tgcatctgac acttggtcag
     9241 cgattttatc tgggtgtcct tctgtaacag actctgaagt aaataatcgt ttgttattta
     9301 acatagtttg ctcctttaaa tttatattac gaaaattctc tctctgtgag ctaaataaaa
     9361 aagaccttct aactattaat atagagagaa ggcctaatac gtccattcgc tcttatcgtt
     9421 cagacctatt tgtctgcaaa cggtttggca cctttctttt ataaaaaaag aggttgctgg
     9481 gtttcattgg gtccatgtcc ctccaccact caggataaga gaatccgtta aaaataatac
     9541 tacctaatta atgaattaat gtcaattttt cacatataaa tttacagtaa aatattgtag
     9601 attaattatg ttaatgtgtt atactaatta aatgtaaagg cttacattta aattatcgct
     9661 ttggagggat ttaggatgtc agtagacaca tacactgaaa caactaaaat tgacaaatta
     9721 ctgaaaaaac caacgtcaca ttttcaactt tcgacgacac aactttataa taaaatctta
     9781 gacaataacg aaggggtatt aacagaactt ggtgctgtta atgcgagtac tggaaaatat
     9841 actggtcgtt cgcctaaaga caaatttttt gtctctgaac cttcatatag agataacatt
     9901 gattggggag aaattaatca acctatcgat gaagaaactt tcttgaagtt ataccataaa
     9961 gtactagact atttagataa aaaagatgaa ttatacgtat ttaaaggcta cgctggtagc
    10021 gataaagata caatgttaaa acttacagtc atcaacgaat tagcatggca taatttattt
    10081 gctaaaaata tgtttattag acctgaatca aaagaagaag ctacaaaaat taaacctaac
    10141 ttcactatcg tttctgcccc acattttaaa gcagatccag aagttgatgg tactaaatct
    10201 gaaacctttg tcattatttc atttaaacac aaagtcattt taatcggcgg tactgaatac
    10261 gctggtgaaa tgaaaaaagg tatcttctct gtaatgaatt acttattgcc gatgcaagat
    10321 attatgagca tgcattgctc agcaaacgtt ggtgaaaaag gcgatgttgc attattcttt
    10381 ggtctatctg gcactggtaa aacaacctta tcggccgacc cacaccgtaa actaatcggt
    10441 gatgatgaac acggctggaa taaaaacggg gtctttaata tcgaaggtgg ctgctatgca
    10501 aaagcaatta atctttccaa agaaaaagaa ccacagattt ttgacgcaat caaatatggt
    10561 gcaatcctag aaaacacggt tgtttcagaa gatggttcag tggactttga agataatcgt
    10621 tatacagaaa atacgcgtgc ggcatatcca attaatcaca ttgacaatat tgtagtacca
    10681 tcaaaagcag cacatccaaa tacaattatt ttcttaactg cggatgcatt tggtgttatt
    10741 ccaccgattt caaagttaaa taaagaccaa gcaatgtatc atttcttgag tggtttcact
    10801 tctaaattag ctggtacaga gcgtggtgtg acagaacctg aaccatcatt ctcaacatgt
    10861 ttcggcgcac cgttcttccc gttacaccct actgtttacg ctgatttatt aggtgaactt
    10921 atcgatttac atgatgttga tgtttatctt gttaatactg gatggactgg cggaaaatat
    10981 ggtgtaggac gtagaatcag cttacattac acacgtcaaa tggtaaacca agcgatttct
    11041 ggtaaattga aaaatgcaaa atatacaaaa gatagtacgt ttggtttaag cattcctgta
    11101 gaaattgaag atgtaccgaa aacaatttta aatccaatta atgcttggag cgacaaagag
    11161 aaatataaag cacaagcaga agatttaatt caacgttttg aaaagaactt cgaaaaattt
    11221 ggtgaaaaag ttgaacatat tgctgaaaaa ggtagcttca acaaataact ttgcatacta
    11281 attaaagagc caaccattgt tatagtctaa caatggttgg ctcctcttat tttatatggt
    11341 aaaaatttat aggcattttt attacaacca tgcacattta aggtgacctt catgccaaac
    11401 tcacatcact cattcaatgg aagcagcacg tcttcacata aagtaccgat cccttacgca
    11461 ttgcatgttg ccccactttg cataaagcgt gtaccgctca ttcaacacat gaagcaccac
    11521 atcttcaagg cttgatagtt cccatgcgca caccacgttt catactacct atgcaactca
    11581 acttggttca taaattcttt aatataagtc aatgtttcaa ccatcgctgg tggtcttggc
    11641 acatgtcctt ctgccatttg ataaaatgtt tcatgcgcag caccttttaa ctctagttgg
    11701 tccgctaaat aatacgcatg atgaatacca acttgctggt ctttccctcc atgtacaatt
    11761 aatattggtg gactgttttc attaatgttt ggaatcgctt gacgtgcctc atatgccgat
    11821 cgatcttttt tcggatgacc aatcattctt cgtaacatcc ctcttaaatc aacgcgctct
    11881 tcatacatca aatcaatatc tgagactcca ccccagatga tataactact tactggtaga
    11941 tcttgaaacg tcaacaaccc ttgtagtcca cctcttgaaa aaccaaccat atgaatgaaa
    12001 gcttgcggat atttatcatg tagcaatctt aatagttgcg tcacatcatt taaatcgcca
    12061 cgataaaatt catctttacc ttcactccca ttgttacctc ggtagtatgg cccgatcact
    12121 aaagtttgac tatctgaaaa ttgcattaat ctacctgcgc gcacacgtcc tacttggcct
    12181 ttgccacctc gcaaatatac tacaatgcgt tttacttcat gatgtggtgt catcattaaa
    12241 gcttttactt gtaagtcatc tgacaaatat gtaatttctt cgaattgatg cgtaaaagat
    12301 tcaattggca ttcgtttacg tttgataaaa tccaagtgat tgcaccctct ctacgcattt
    12361 taaaatggta ctatcttgca gtaagaaact ccgttgtgcg agttcaatat cattgataca
    12421 gttaaacaac actggccctg ctgtttctaa ataatcgttc ttgcttacca atgattcaac
    12481 ttcgataaaa tatacatctt ttacaaaatc agtttgatca tgtgtttcaa tggtatattg
    12541 tgctatgtaa tgaatatttt taactttggc gcctgtttct tcatataatt cacgggtaac
    12601 tgcttcagca ctactttccc cgcgttccct tttaccacca ggaaattcaa taccccgtaa
    12661 attatgtttg gtaaaaagca attgattttt aaacgttgga atagctagca catgattgcc
    12721 atctgctatc tcattatcct ttttaaatgt caaattaact tgacgattat ctttatccct
    12781 aaacttcacg cgcatcacat ccctacattg tatgttaata taatagttaa ttactatcgt
    12841 tggaggcatt aattatgaaa aagatattct tggcgatgat acatttttat caacgtttca
    12901 tttcgccact cactccacca acttgtcgtt tttatccaac atgttcagag tacactagag
    12961 aagcgattca ataccacggt gctttcaaag gcctttattt aggcatccgt cgtattttaa
    13021 aatgtcatcc gcttcataaa ggtggctttg acccagttcc gttaaaaaaa gacaagtcag
    13081 caagcaagca ttcacataaa cataaccatt aatatggttg taattgagtt atatctacta
    13141 aaggggggcg aaattcgagt cgcccctctt ttaatatgcc tgaatgcgcc accacatctt
    13201 gttcaaaata ataacctgct ggtgtaacat ctcctggata atcaccttta cgagcaagca
    13261 tcgctgtaaa ataacggctt aaaccatatt cgtacatgcc gccaattacc acttttacac
    13321 catgactttt taaagtatca attgccgttt gcactttatc aatgccacct agacgaaatg
    13381 gttttaatac aacaactttc acattgtata attctatcaa attaattatg gccgacagtg
    13441 acgtcgcctt ttcatcaaga gcgattggag gagttttttc agccactact tcatcaatca
    13501 ttgaaatttc tttaaatggc tcttcgatat aaagtatatt ttcatgtgct aataattgta
    13561 actgtgtcat gtcttgacgg gtcaatgact catttgcatc tataactaat tgaaactgaa
    13621 agtttaactc acgtaacgat tttatttgat ctataatttg aggcgtccat tttaatttaa
    13681 ttctggcagg ttttgtttct ttcaaggcag ctagttgttt gttagataac ccacttactg
    13741 tcgcaccata tgctactgaa aatgacggta aagtgtgaaa catttgatac aatgccatga
    13801 caacagttgc tcttgcagca ggagtatctt ccaaaggtga caataactca agtgccgctt
    13861 catacgattc gaatgattta tttttaatat ctatgaacca ttgttgaagt gcttctttta
    13921 ctgaatcaat cgtttcaaaa tcataccaat ctgtttgaaa agcgttacat tccccgaaat
    13981 atgcattgcc tttatcatca attatttcaa taaacaaaca atcacgatga gttaaagtga
    14041 ctttcggagt tacaatttta gacttaaatg gttcactata tttataaaaa tgcacagccg
    14101 tcaatttcac taaatcaccc tctattcaac ttattccttt gtaatttacc agtcgatgta
    14161 tatggtaaag tatcaacctt ttcaaagtat ttcggtactt tatatttcgc taaatgctga
    14221 gacaaatatg caatcaattg cacttcagaa atgtcacttt cactgacaaa atataattta
    14281 ggtacttgtc cccaagtatc atcaggatgc cctacacata ctgcgtcact gatacctgga
    14341 aattgcttcg ctaccgtttc aatttgatat ggataaatat tttcaccgcc actaataatt
    14401 aaatctttac gtcggtcata aatcatgaca taacctgcat gatctatttc agcaatgtca
    14461 cccgtattaa aataaccatt ttcaaacgta tccgttaaat ctgttggata caaatatcca
    14521 ttcattacat tggcgccttt aatcattaat tctccatgac cttctttatt aggattttta
    14581 atttttacgt caacattggc acttggcatg ccaacggtat caggacgttc atgcaacatt
    14641 tccggtgttg ctgttaaaaa ttgcgaacat gtctcagtca taccaaatga attataaatt
    14701 ggcaggttat attgtaatgc cgtctctatc atagtggcag ataatttagc accgccgagt
    14761 aatatttttt gtaaatcata aggttcatgc aaaccttgtt gcataagcca atttaaagtt
    14821 tgtggcacaa gcgaaatgtg cgtgatgcgt tcatttttta tcatcgttaa aatttgttcg
    14881 gcattgaatt tatcaacaat gcgcacagta aacccttcaa taacagctct taaaagtaca
    14941 ctgagacccg aaatatgata aatcggcaag acagatagcc aattagtatc acgatcaaat
    15001 cccaagctct ctttacatcc gattgcactg gcataatgat tacgaaacgt ttgtggcacc
    15061 gctttttgag ggccagttgt ccctgatgta aacataatcg atgcaatgtc atctaaatta
    15121 aatgatgtat ttaatatgtt ggacggcgaa cctttcggca ccacagtttc attagatgta
    15181 tcatattgga tgcccaatgt gttgtccatt aaatcgttca ttgtaatatc cgttccagcg
    15241 aattcaatat catccaacga tacaatttga aaccctcgca attccagtgg caaggtacaa
    15301 aaaatcaatt gtacatcaat tgacctcatc tgatttgtca tctcattagg tgtcaacctt
    15361 gtattaatca tcgcaatttc aatatttgcc aaccaacatg catgtattaa aatgatcgat
    15421 tgaatcgaat tatctatgta taacccgaca cgagattgtt gataagtctt gagtctttta
    15481 gccaatagac tcgcttcaca gtataaattt tgataagtat aagattcttg accgtctgtt
    15541 atcgcaatat gatgtccatt ttgttgtgct tgtttatata accaaaagtc catgcgctat
    15601 tcctccaaaa tcatttacat tataattata acgattttat gacattctag cagtgcttat
    15661 gtttaaaaat ataaaaaagt agacgaattg attcattgat atgattgtta taatgctcaa
    15721 tacatatcgt tatatcattc gtctactatt atcagttatt tttatttaat tttagtgtca
    15781 ttctgtcatt ctgatgtggt gatttatcca ttgttgccac atcatctgca atgtcaatcg
    15841 gtctatgatt catatcttgt aatgcactta aatgaaatag ttcatcatct aaattttcaa
    15901 tgagatatac ataatatgtt accttgtcct ttttatattt taacgttttc caaaagtccg
    15961 gcttgcaatt caatacatta tccggaatat attcaataaa taagtaacgt ttgctgccta
    16021 ctttgtctac gaaatatttt acagtgcctt tttctatacc tcttatatgt gcatagtctg
    16081 ctgaaaagta aatactacct attgtttcat tatgttgttg tatttcaaat cgttggccta
    16141 ctattttatt atttgtgcca caactactta aaaaaatcag tagatatagc attaaacata
    16201 ttttcatccc cttgaatttt aaaaactttt tcaaaagcaa cacctctaaa aataaataca
    16261 atatattata tcaaatagaa attattattt tgttaaatta tgttgtcatg ttcgtgtcat
    16321 tttgagtcgt aatacaactt tacaattatt aaattaacac ttctacaatc caaaaatatt
    16381 tttaaagttt tgaaaaaaat taaagtattt ccactactaa tttggtaaaa tatgattatg
    16441 tgatatagtg aaagcgtttt acactaaata attccctctt aaatgcatcc aggttttatg
    16501 tagtaaatca tgaaaataac atataaatat agaggagatt tacctttgaa tacagagaac
    16561 aacaagaatc aaaaccaatc tgctaaaaat tctgaaagac gtggcatgtt aaaaggatgc
    16621 ggcggttgcc ttatttcttt tattttatta attatcttat tatcagcctg ttcaatgatg
    16681 tttagtaata atgacaattc cactagtaat gaatcatcaa aaacgcaatt aactcaaaaa
    16741 gacgaagata aaagtgaaaa tatgcctgaa gaaaaatcag aatcagaaac agataaggat
    16801 ttacaatcaa ccgaagaagt acccgcaaat gaaaacactg aaaataatca acatgaaatt
    16861 gatgaaataa caacaacaga tcaatcagat gatgaaatta acacaccaaa cgttgcagaa
    16921 gataaatcac aagacgactt gaaagatgat ttgaaagatg atttaaaaga aaagcaacaa
    16981 ccaagtgacc atcatcaatc cacgcaacct aagacttcac catcaactga aacaaacaag
    17041 caacaatcat ttgctaattg taagcaactt agacaagtat atccgaatgg tgtcactgcc
    17101 gatcatccag catatcgacc acatttagat agagataaag ataaacgtgc atgtgaacct
    17161 gataaatatt aaacaacaag cgaattgaat tcaaattgta tttagcttta tgcactaatc
    17221 acatagtaaa taatgaggga gattttttag gcatgagcaa tcaattcaaa agcgaagaag
    17281 agcgaagaca atgggaacaa ttccaagctt tccaaaatca acaaaaccaa cagcaccagc
    17341 aatacggaca aaagaaatct aaaaaaggat ggttctgggg ctgtggtggt tgtctagtat
    17401 tatttatttt aattatcatc ggtatttcag cttgtacagc tggtgtcact ggtaaccttg
    17461 gcggaaatag ttctaaagaa acgaacaaaa cccataaaat cggtgaaact gttaaaaatg
    17521 gcgaccttga agtcactgta aattcagtgg aaactatgaa atctgtagga ccatctcttg
    17581 caccaacaaa cgctaaaggt atatttgtcg ttgctgatgt gacgattaaa aacaaaggta
    17641 aagaagcgtt aacaattgat agttcaatgt ttaagctaaa atccggtgat aaaacatttg
    17701 aagcagataa tacaggttca atgtctgcta atcaaaatga caatggtagt atagaaaatt
    17761 catttttctt acagcgtata aatccagata gcactgctca aggtaaaatt gttttcgatg
    17821 tgtcagaaaa catagccaac gcaaaagata aaaaattaga agttatttct agtttattta
    17881 gcgtcaagaa gattacattt gatttatccg atgctaaaaa aacatcaaaa gctaaaaaag
    17941 acaagcaaga tacagaagta gctgttgcga gttcaaatag cgataatgta agttatgaag
    18001 cttcggctac tacacctgct acaacttcta gtgcggatac tgattctgaa gatagcgaaa
    18061 agtctagtaa agatgaggat aagcagaatg cgtctaaaag tgataaatct agtgtagaaa
    18121 aaagtgaatc taatgaggaa actgctcctg tagagcctat gcgccagagc aaacctacca
    18181 ctagtgaagc accacctagc caaaatattc acaatgaaga tagcatgtac gacgcttcaa
    18241 cagaataaaa tttcagggta gctcggctac ccttctttta cggaaaaatt aattatacat
    18301 aatcaaatca aggagataaa aaaatgaaat tcaaagctat cgttgcaatc acattatcat
    18361 tgtcactatt aactgcctgt ggtgctaatc aacataaaga aaatagtagt aaatcaaatg
    18421 acactaataa aaagacgcaa caaactgaca acactacaca gtcaaataga gaaaagcaaa
    18481 tgacaccaca agaagccgaa gatatcgttc gaaacgatta caaagcaaga ggtgttaatg
    18541 aatatcaaac attaaatttt aaaacaaatc ttgaacgaag taatgaacat gaatattatg
    18601 ttgaacattt agtccgcgat gcagttggca cacctttaaa acgttgcgct attgttaatc
    18661 gacacaatgg tacgattatt aatatttttg atgatatgtc agaaaaagat aaagaagaat
    18721 ttgaagcatt taaaaagaga agccctaaat acaacccagg tatgagtgat caagctgaaa
    18781 cggataatga gtcggaagac attcaacatc atgatattga caataacaaa gccattcaaa
    18841 atgatacacc aaatcaaaaa gtcgatgata aaaacgataa aaatgctgat aataaagaag
    18901 aaaaacacga taaccgtgaa aataattcag cagaaactaa agttaaataa gggtatactt
    18961 tgattaattg taatttttag gagaaacgct atgataacat ttgagaatat acaacaactt
    19021 gagaaataca ccttaatgac tatgcatggt ctttttaatc aacttaaact cggtattatt
    19081 tcaatcgaca atgcagagca tacgctcttt acaccttata tgatggaaac actctcttcc
    19141 ctaggtgtga aacgagacat tatcaattta attcacaaag ggacagagtt agaagacttt
    19201 gcggcattta atttatcaat tgaagataca gttacagtct gtttacaaag aactgaagaa
    19261 ctattaaatc aatacaaaaa tgtggaattc aatgacaaaa tattaatcaa ttggcgcatt
    19321 atgcaagaga aatagacata taaaagtcga acgtaactat gtggatattg attttattct
    19381 ttctaaatta caagtatttc attttataaa gtttgaaaag aggtatattg aaatggagaa
    19441 aaacgaatat acagctaaat ataatgaata tagtcaatta ttagacgtta cttactcgca
    19501 agctgtagca taccttttaa ataaatatgg cgctgtaacc gatgactatt ataaggaaaa
    19561 atcatacaca cgatttttaa atggagaaat caaaagtatt tcaaaaggaa aatacactag
    19621 agctagcgaa ggattatatt gtcatcacat aagcgaggac aaattccaaa acttatctga
    19681 cctaagattc atttccgaat ttaagtactc atacaattat caaaagaaag aaaacttagt
    19741 ttactgcgat ttaatcgagc atttaatttt acatgcaatt attacaaaag aatccaatgg
    19801 ccaatttggc gtggctggat tatgtcaaat gatcaaacca acagtcattg attggtacat
    19861 tggcgaatat aatccaaaac cagcatggat gcaagccacc aaagcacgtg cctatttgcc
    19921 tagaatatta gtagagaaat tactcattaa aattgacgat atgttaaaag gaatagaaat
    19981 atatgatttc cttgagtcgc gataaatgat taatggatat ttattttttg ctgttgggat
    20041 tttgttatag atgtttaaac ccgtaattaa atatatttta taaaaataga ccacgcatac
    20101 ctatctataa acggacaatg tttataaatg agtttgcatg gtcttgaatt gtattagttt
    20161 tcttttggtt ttaataaatc gactagattt tcacaatatt tatcaaatat gtattcctaa
    20221 attatacagc cttaatccag cagctacttt cgaaacttcc aacttagttg atataaggtt
    20281 caatagtttg tttcgttctt tttcagataa accagaactt aaattgatat tattgacttc
    20341 ataaaaatta tagactaatg cctctatttg ctttttaggc ataagtaagt cgactgaaaa
    20401 ctgatttacg tcgctttcat aaatcatttc atgcaaattc tttagactat tatcgttgtt
    20461 atctttaatt ttgtataaat aacggccgag ttcacgagct attgcaaatc ttgtattatt
    20521 aatcgagaga ttattattga tataaatcgt
//
LOCUS       DAPIDM010000035        20421 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000035 DAPIDM010000000
VERSION     DAPIDM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.035"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1016)
                     /gene="clpX"
                     /locus_tag="RJE42_001134"
     CDS             complement(<1..1016)
                     /gene="clpX"
                     /locus_tag="RJE42_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDV5876565.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELD"
     gene            complement(1167..2468)
                     /locus_tag="RJE42_001135"
     CDS             complement(1167..2468)
                     /locus_tag="RJE42_001135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV5876566.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(2516..2626)
                     /locus_tag="RJE42_001136"
     CDS             complement(2516..2626)
                     /locus_tag="RJE42_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876567.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(2631..3560)
                     /locus_tag="RJE42_001137"
     CDS             complement(2631..3560)
                     /locus_tag="RJE42_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876568.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(3579..4187)
                     /locus_tag="RJE42_001138"
     CDS             complement(3579..4187)
                     /locus_tag="RJE42_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDV5876569.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(4329..4685)
                     /gene="rplT"
                     /locus_tag="RJE42_001139"
     CDS             complement(4329..4685)
                     /gene="rplT"
                     /locus_tag="RJE42_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV5876570.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(4732..4932)
                     /gene="rpmI"
                     /locus_tag="RJE42_001140"
     CDS             complement(4732..4932)
                     /gene="rpmI"
                     /locus_tag="RJE42_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV5876571.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(4961..5488)
                     /gene="infC"
                     /locus_tag="RJE42_001141"
     CDS             complement(4961..5488)
                     /gene="infC"
                     /locus_tag="RJE42_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV5876572.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(5517..5642)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(5717..7210)
                     /locus_tag="RJE42_001142"
     CDS             complement(5717..7210)
                     /locus_tag="RJE42_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5876573.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGMMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGGNDVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFETFGKTNKNGVPYMSLLVTAIIVVIIFVVQSVTEGAYQYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKWFPFGPIFAAILCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(7636..9573)
                     /gene="thrS"
                     /locus_tag="RJE42_001143"
     CDS             complement(7636..9573)
                     /gene="thrS"
                     /locus_tag="RJE42_001143"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV5876574.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTEPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNIDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(9771..9893)
                     /locus_tag="RJE42_001144"
     CDS             complement(9771..9893)
                     /locus_tag="RJE42_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876575.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPAQDRYLKQRYLIHNLY"
     gene            complement(9986..10906)
                     /gene="dnaI"
                     /locus_tag="RJE42_001145"
     CDS             complement(9986..10906)
                     /gene="dnaI"
                     /locus_tag="RJE42_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDV5876576.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(10906..12306)
                     /locus_tag="RJE42_001146"
     CDS             complement(10906..12306)
                     /locus_tag="RJE42_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDV5876577.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLIEHENQMPKL
                     QVKSPATSSSTAKSTEANPMPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEQDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(12307..12777)
                     /gene="nrdR"
                     /locus_tag="RJE42_001147"
     CDS             complement(12307..12777)
                     /gene="nrdR"
                     /locus_tag="RJE42_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDV5876578.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(12987..14012)
                     /gene="gap"
                     /locus_tag="RJE42_001148"
     CDS             complement(12987..14012)
                     /gene="gap"
                     /locus_tag="RJE42_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV5876579.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSENKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(14182..14805)
                     /locus_tag="RJE42_001149"
     CDS             complement(14182..14805)
                     /locus_tag="RJE42_001149"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV5876580.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(14821..15693)
                     /gene="mutM"
                     /locus_tag="RJE42_001150"
     CDS             complement(14821..15693)
                     /gene="mutM"
                     /locus_tag="RJE42_001150"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV5876581.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFIEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(15709..18339)
                     /gene="polA"
                     /locus_tag="RJE42_001151"
     CDS             complement(15709..18339)
                     /gene="polA"
                     /locus_tag="RJE42_001151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV5876582.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASGEDAIEKTFEIETSFDNVDFTSLEEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAF
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIYTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKVFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(18631..20118)
                     /locus_tag="RJE42_001152"
     CDS             complement(18631..20118)
                     /locus_tag="RJE42_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876583.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLSFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTTLPYGVIFSQVIILSMIATLIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFANIIVCGYFAFS
                     IFVGIYLSINNRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAMIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         6303 a         3866 c         2774 g         7478 t
ORIGIN      
        1 tcatctaatt ccagcatttt agtatattgt ttcacaagtg catttttagg ttgcgttaag
       61 atgtttttca acgcagttac atctaatgtt tctaaattag ctacaattgg cacacgtccg
      121 ataaattcag gaatcaaacc ataggcttgc aaatcttctg ggcgaatttg tgctaataat
      181 gcttgttcgt catatttatc agcttcattg cttgagaaac caataacttt ttcaccaaga
      241 cggcgcttaa tcacttcttc aataccatca aaggcaccac caagaataaa taagatattt
      301 gttgtatcaa tttgaatcat ttcttggttt ggatgtttgc gtccaccttg tggcggaaca
      361 cttgcagtcg taccttctaa gattttaagc aatgcttgtt gaacaccttc acctgaaacg
      421 tcacgtgtta tagatgtgtt ttcagattta cgtgcaattt tatcaatttc atctacataa
      481 ataatacctt tttcggcttt atcaatgtca aagtcagctg cttgaattaa tctcaacaag
      541 atattttcaa catcatcgcc tacataacca gcttcagtta aacttgtcgc atctgcaatt
      601 gcaaatggta cattcaacgt cttggctaat gtttgagcta ataatgtttt accactacct
      661 gttggcccaa ttaaggcaat gttacttttt tgtaattcaa catcatcttc ttttggtcct
      721 aattgttgaa tacgcttata gtggttataa acagccacag ctaaagattt tttagctttt
      781 tcttgaccaa taacatattc gtttaaatga tccataattt ctttaggagt aggtaattct
      841 gtaatcgctt cagaagtgtt ttgagctaat tcttcttcga cgatttctga gcataattca
      901 atacactcat tacaaatata tacaccactt cctgctacaa gtttttttac ttgatcttgg
      961 tcttttccgc agaaagagca tttcaaattt tcttcatctt cattgaattt aaacattctt
     1021 tttacacccc tattcgttaa agactatact agattggata ttacaatgca acatattaac
     1081 atacaaactt tttgcttaaa gaatagtagc agatacataa gctatgcatc tgctactcat
     1141 ttttgtaatt taatatttaa tgaagattaa tcttctttag ttccttcaac gaactttgcg
     1201 ttatctctta ataaatcgat aactttttgg atacgaacat catttttaat gatatcagta
     1261 ttacctaaag tatttttgat atcttcaact gagatattaa attgtttact cattttttct
     1321 aattctttat cgatatcttc atcagtagct tcgatttttt cagcttcagc gatcgcagtt
     1381 aaagttaagt tagttttaac acgttgttct gcatcgtctt tcatttgctc tcttaattga
     1441 gattcatctt gacctgagat ttggaagtac gtttgtaaat ctaaaccttg ttgctgaatt
     1501 ctttgtgcaa attcagacac catacgatct aattcagtat taaccattgc ttcaggaata
     1561 tcgattgttg tattatcagt agctttagta atcgcttctt ctttttcaac attttctgca
     1621 tctgtagctt tttgttcagc taaacgttta cgtaagtttt ctttgtactc gtctactgta
     1681 tttgcttctg catctaattc attagcaatt tcatctgtta attctggaac ttccttgaat
     1741 ttaatttcgt taacttttgt tttgaaagtt gcttctttac cagctaattc ttcagcatgg
     1801 tattcttctg ggaatgttac gacaacatct ttttcttcgt caactttcat accttctaat
     1861 tgctcttcga aaccaggaat gaatgaacct gaaccgattt ctaaatcgta accttcagct
     1921 tgtccacctt cgaattcttc tccgtcaact gaaccactaa agtcgatgtt aactgtgtcg
     1981 ccattttcaa caacaccatc ttctttaact accatttcag ctaaatgtcc taagctgtgg
     2041 tcaattgctt cttgtaactc atcatcagat aattcagttt cttgtttttc aatttcaaga
     2101 cctttatagt ctcctaattt aacttctggc tcaactgtaa ctgttgcttc aaaaatgaaa
     2161 tctttacctt tttcaatttg agtaacactt acttctggtt gtgcaactgg tttaatatca
     2221 gtttcgtcaa ttgcttcacc ataagcatct ggtaataaaa tgtcgatagc atcttgatat
     2281 aatgcttcta caccaaagcg ttgttcgaaa attggacgtg gtactttacc tttacggaat
     2341 ccaggtacgt taatttgttt aaccactttt ttgaatgctt gatctaacgc tttgtttact
     2401 ttttctgcag gaacagtaac agttaataaa ccttcgttac cttccttttt ttcccaagtt
     2461 gctgtcatgt atatatacct ccatgattaa ctaatttatt ttttcaactt ccctattata
     2521 tcataggtct attccctata caaacattga aatcacaacg tttatatatt tgtaaatcaa
     2581 cttttttcgt caaaactata ttaaaatgct gagtattatt ttgcaacaat ttaactatta
     2641 ttattcaaat ctaattgctg aataaaatta aatgcatcga tactttgtaa ttcacattta
     2701 agtcctaaca tatttttgaa ataacattcg tatgcattaa tccatttatt cgtttcaaac
     2761 aaagtttcaa tatcaattgg ataaatcatg atagagtgat tattcataat atggtgtgct
     2821 tcttcagcga tatgtaaagc accatcattt aatgtttcca taacgttagg tataactttt
     2881 tctttcagtg ttgtatgttc tagccctttt aaattagctg gtacaaaagt tacatccata
     2941 ccatactttt caattgtcag ttcttgtgta caatttgcga accttaaata ctcaatcatt
     3001 aaactaatta ggttataact gtacgtatta gattttaata tatataatac cgtttcttga
     3061 aattgaaaat gaccattgtc tattaacttc aaaatcaagt gcgtctgttc cctcattgat
     3121 aatgtatcaa aatcagttaa tgactgagtc aatcgttttt catcttcaat taattttgac
     3181 ttagcaaatt ccttaagagg atgtaatgcc attcttgttt tgtgatcttt gacttcatca
     3241 ataatttgat gaattacttc taccgcttca aaatattgtc ccaaaccaat caaacttttc
     3301 acgtaataaa tcatcaatgc atcatattgt tgtatgccag ttttcaataa cacaattgtt
     3361 tcttctctta attcaagaaa tgaccccgtc tcatataaca tacggcattt catcattgct
     3421 atttcttcag tgagttcaaa ttgacgttca tattgtacaa tatattcata catcaggtca
     3481 tagttttggt cttgttcagc acgttttata tctttatata actttttcga agagttagga
     3541 aactgaataa tatctgacat atatgtcacc tcgtttgatt ataaatcttg aaggattaat
     3601 gcgctccacg tctcgaaatc atcatatgac tctatgttgc ctttttcaac ccattttatt
     3661 cgtaaagtat ctgtttcttt agcttctaca tcattcgtac ttacagtgat tttaaataca
     3721 acaccaatat gtaccttgcc cacttcgtta ttatcgtcat taataaaacc gatatattcc
     3781 atattttgtg aatcttgctc acttaaacct acttcttctt ctaattctct ctgtgcatta
     3841 actctcaata cttcgttaat agattctgct cctggaacat cattcatatg accgcctaca
     3901 cctattgaag attgtccatg caatcgagct tctccaccgc cagataatcg ttcatacact
     3961 aatatctcgc catgctcatt ttcaagtaaa caataagaaa tgagttgttt atatgatgga
     4021 tcctcttcca tatcgccgcg tcgcttaact tcatattgac ttagcgcatc aaaaatattt
     4081 tgaccttctg gtttattctt atttaaaaac ccattgaaag tattcttttc attattaaaa
     4141 ataatttctc taggtactac aatgatttgt tcatcaaatt tagacatcgt ataaaactcc
     4201 tcactttaat gtttgttctc ttaaatgcat tttagcaaat atgtcatatt ttagcgatta
     4261 aatgtggcat tttgctattt aaaaaataaa aaagagatga caatgccatc tcttttttat
     4321 aagtgttatt attttaaagc atctttagct ttagttacta attgagcaaa tgctttttcg
     4381 tcagaaattg caatttctga taacatttta cggttaatgt cgataccagc ttttttcaaa
     4441 ccgttcatta aacgtgagta gctcatttca tgttgacgag ctgctgcgtt gatacgtgta
     4501 atccataatt tacggaagtc acgtttacgt tgacgacggt cacggaaagc gtattgacct
     4561 gatttcatta cttgttgttt agctactttg tataatgtat gttttgaacc gaagtaacct
     4621 ttagctaatt taatcgtttt tttacgacgc gctcttgtta ctgttccacc tttaactcgt
     4681 ggcataaaaa attcctcctt aggtaattcc taccgatttc tgtatttgtt cttatttttt
     4741 gtatgctaat aattgtttta cacgtttcat gtcgctctta gacactaatc tagctttacg
     4801 taattgacgt ttttgtttag tgctcttgtt tgcgaataag tgagatgtga aagctcttga
     4861 acgttttaat tgacctgaag cagttctttt aacacgttta gctgctccgc ggtgagtttt
     4921 catttttggc ataatataaa tcctcctgta atagctttaa ttatttttca gctgttggcg
     4981 ctaacatgat aaacatttga cgcccgtcca ttttaggttt ttgttcaact gttgctatat
     5041 ctttgcattc atctgcatat ttttctagca cacgttgacc aatttcctta tgcgtaatgg
     5101 cacgccctct gaaacgaata gatactttac atttatcgcc tttagttaag aatttacgtc
     5161 cgtttttcaa cttagtttgg aaatcatgtt cctcaattgt tggacttaaa cgaatttctt
     5221 taacattgat aattttttgt ttctttttca tttctttttc ttttttctgt tgttcgaatt
     5281 tgaatttacc gtaatccata attcttgcaa ctggtggttt cgcattaggt gcaacgacca
     5341 ctaagtctaa atctacacgt tcagccattt ctaaagcttc acgctttgat ttaacaccaa
     5401 tttgttcacc atcttgaccg attaaacgta attcttttgc acgaattttg tcattgattt
     5461 gagtttgatc ttttgctatg gttgacacct ccaaaatttt tacgaaattt gcaccaagca
     5521 aaaaggaaga gcaggtataa aatacccgct cttccttata cacagttatg tgtaatgtga
     5581 ttaacctgcc aactgcttta tgcgtcgcta caggtgagaa gcgggtgctt ctacttggtt
     5641 cgtttcgtat tcaacgttgt taatcatacc aacatttcac atttaagtca acactataac
     5701 tgtaattatt tttattttaa ccttttattt catccattga cacgtcttga cgtaaatcta
     5761 cttgttctaa tggaattttt ttcgttttat atcgaagctt atgataaata aagaatgcta
     5821 aaaatactgg aattcccata tacgtaatta agaagcggct aaaattaaaa tctcctgttt
     5881 taataaagtc cacatcttga ccgatgatta caacaataca taagatagct gcaaaaatag
     5941 gtccgaacgg aaaccattta gctgtatatt tcaatttaga tttatcataa ttttgtttat
     6001 caaatgctct tctaaatcga taatgactca ctgcgatacc tacccaggca ataaagccag
     6061 ttaacccact tgcagcaacg atatattgat aagcaccttc tgtaacactt tgtaccacaa
     6121 atatgatgac tacaataatt gcagttacta ataaagacat atatggcaca ccatttttat
     6181 ttgtttttcc aaatgtttca aacgccaatt tatctttact cattgaataa agcatacgcg
     6241 ttgaggcata cattccagag ttacctgctg ataaaactga tgttaagata acagcattca
     6301 taaatgaggc tgcaaaagca aatcctgcat ttttaaaaac tagcgtgaac ggagaggttg
     6361 caacgtcatt accgcctccc attaatgcac tactatcata aggaattaac ataccgataa
     6421 caaaaatggc taaaatgtaa aataataaaa ttctccagaa tacttgttta attgctttcg
     6481 gcacagcacg ttcaggattt tctgattcac cagccgtaat accaattaac tcagtacctt
     6541 ggaatgagaa accagcgatt aagaatacac ctagaattga taataaactt cctcctaagt
     6601 tgccacctaa aataggtcct tcacctttat taaatatttc gaatcctaca acatgaccac
     6661 ccatgattcc gacaatcgtt aataaaccaa ttgcaatgaa aacaataact gtaaccactt
     6721 ttatcaatgc caaccagtat tcactttcac catagacgcg aactgataac gaattcagac
     6781 taaaaatgat aactaaaaac aacgcgctcc atgcccaagc tggtattcct tgtaatggtg
     6841 tccaatattg aatgacttgt gcagcaatcg taatatctgc tgctactgtt actacccagt
     6901 taaaccaata gttccaacca agcgcaaatc ctaaagatgg atctacaaat cttgtagcat
     6961 atgtactaaa tgaacctgat actggcaaat acgtagccat ttcgccaagt gacgtcatta
     7021 agaaaaatac catcattccg ataattgcgt atcctattaa tgcacccaaa gcacctgcat
     7081 catgaatcgc tccaccagaa gttacaaata aacctgtacc aatacaacct ccaatcgcga
     7141 tcatagatat atgacgatct ttaagtcccc ttttgacaac attgttactt tcattttgaa
     7201 cttttgacat ataagctccc tttccatgtt ttagaggcca agaaataaat aacctttatt
     7261 cactttcata ccctttttta cagaacatag tgatattaaa agtaaaaaag tggctacata
     7321 cctttaagat atgcaaccac tttggcaatt atctatataa ggtagcacat cacattctgt
     7381 gacagtacag ttcctattcg aaaactgtcc caatagatat aatttatggt ttatatctat
     7441 ttcggcatct ttacctttca cttgttcaac ttatgtacca taaatacttc tgacaagtta
     7501 ctaattaaac atgcaacctc taactcaatt taatatttta actaacttgt aatatacagg
     7561 attcatcacg cataatcaac cctgtaaaac ttgatacgca ataaaaattt caaagcattt
     7621 tattgcgaca actgtctatc tatgtttttt caaacgaatt tcatcaacta gattccagat
     7681 aaattcatct ttttcaactg tttcttggtc ttgtgatcca tattgacgca cattcacttg
     7741 attattttca acttccttat ccccaactac gatttgataa ggtatttttt gcatttgagc
     7801 ttctctaatt ttataaccca ttttttcatt acggtcatca atacttacac gaacgccttg
     7861 agatttcaat tcatcttgta attggcgcgc ataatcataa tgtaaatcaa tgttaactgg
     7921 aatgatttga acttgttttg gcgctaacca agttgggaat gcacctttag tttcttcagt
     7981 taaaaatgct acaaatcgtt ccattgttga tacaacacca cgatgaataa caactggacg
     8041 atgatgttca ccatcttgac caatataagt taaatcaaaa cgttctggta ataagaaatc
     8101 aagctgtgct gttgataatg tctcttcttt acccatcgct gttttaactt gaacatctag
     8161 tttaggacca tagaatgccg cttcaccaat cgcttcttca tacgataagc caagctcatc
     8221 cgctgcctct ttaagcatat tttcagcttt attccacatg tcatcatcat caaagtactt
     8281 ttctttatct tcagggtctc tataacttaa tctaaagcta taatcctcga aaccaaagtc
     8341 tttatacaca tcaataatca tgtttacaac gcgtttgaat tcttctttaa tttgatcagg
     8401 tcgaacaaag atatgtgaat catttaaagt cataccacga acacgttgta atcctgatac
     8461 agcaccactt gcttcatatc tatgcatagt tcctagctca gcgatacgga taggtaattc
     8521 acgatatgag tgtggtttat tcgcataaat catcatatga tgtggacagt tcattggacg
     8581 taataccata gattcagttt catctaactg cattggtggg aacatatctt cttgatagtg
     8641 atcccagtga ccagatgttt tgtataaatc aacattagca agtactggtg tataaacgtg
     8701 gtcatacccc atgctcactt ctttatcaac aatgtaacgt tcaatttcac gtctaattgt
     8761 tgcaccgtta ggtaaccata atggcaaacc agcaccaact aattggctat ttgtgaataa
     8821 ttctaactct ttaccaattt tacgatgatc acgttcttta cgctcttcta acatttgtaa
     8881 atgtgctttt aattcttttt tatcaaagaa agcagtaccg tatatacgtt gtaacatttt
     8941 gttgttacta tctccacgcc agtatgcacc tgctgtagat aatagtttaa actctttaat
     9001 tttagctgtt gatggaacat gaacgccacg acataaatca gtaaaatcac cttgactata
     9061 taatgttaca ttttcatctt ccggaatcgc atcgattaat tctaatttgt attcatcatt
     9121 gctgaataac tctttcgctt catcacgtga aaccactttt cgttcgattt tcatattttc
     9181 attaacgatt tgtttcattg ttttttcaat ttgttcaaag tcatcagatg agatgttttg
     9241 gtcaatgtcg aagtcatagt agaagccacc ttctattaca ggaccaacac caaatttaac
     9301 attaccatat aaccttttaa tcgcatgtgc cattaaatgt gcagtagaat gacgtaatac
     9361 ctctaacgct tcttcactac ctggtgtcac aatttcaatt gacccatcag tttcaagcgg
     9421 ttcagttaaa tctacaagtt gcccgttaaa tttgccggca actgcttttt tacgtaatcc
     9481 aggactaatt gattgtgcta tatcttcagt agtagtacct ttatcaaacg cctttttatt
     9541 accatctgga aattgaatat taatttgttc catgttaaac cctcctattt gctttataaa
     9601 tacttttatc tgacctcgtt aaaaaattga acaacaatta tcgttttaac attagaccta
     9661 ttaacaataa acagggaatt tatgacacca acaaacattt ttgtctgaaa aaaacaaaaa
     9721 atcccgcccc tatacaaggg acgagatcgt cgtggtacca ccctagttat ttaatacaag
     9781 ttatgtatta aatatctctg cttaagataa cggtcttgag ccgggtattc attacaaata
     9841 ccataaatga agtagtaatc atctaattta ttaaccatat tctcatcaaa caatggcttt
     9901 ctgtgtaata ttgattagat catcttgtct tcaatcattt aaacgattta gatttgtatt
     9961 cattatacac caatcatttt aaaattcaat tgtttctgaa attttctcct gataaaaagt
    10021 atggtgttga caaagatttg acacgttcaa taatacgtgc tgctttagtc ttctcttcac
    10081 catcacgagt catcgctaaa tgatgttcca attcactata gtcaaaatta gaactaaaga
    10141 atgttggtaa ttcatgaacc attcgataat gtagcaaagg tccaattacc tcatctctca
    10201 cccatggagt cacttcttct gccccaatat catcaagcat taaaatgttt gcttctctta
    10261 cgcgatgtaa tttcttttca aaagaaccat ctttaaagcc accttttaat gttctaataa
    10321 attccggtaa ataaataatt gtcgaacgta ccttcttaga tttgagttga ttcgcaattg
    10381 cacctagaat aaaagattta cctgtcccaa atggaccata aaggtaaagg cctttcactt
    10441 gttccccatt agttattgct gtacaaatat catctgctgc catagctaca tcaagacggt
    10501 ctcgatgatt catataaata tctttcaatt tggcgtttaa agtatctcgt tgcatatgat
    10561 gagatgtaat taactcagct tcaaagcgtt cttcgtcgta cttgatttta catggacatt
    10621 gtaaatagcg tattttaatt cggttattat caacatataa ctcaggcaca tgccccttta
    10681 cgaaatttgg acaatcagca aatttatgac cgtcataatg tttttgttga tctttatact
    10741 cttgtaacac atttaagtct tcatcaatca tagcattcgt taattcagct cgatgcgctt
    10801 ccaaaaattg cttaacatct gggtcattga ttacttcttt ttttatcttt tctattcttt
    10861 tttcaaagtc ctgcgacgtg ttaattatac ttttaaattg cttcattatt gactgtcctc
    10921 ctcccatttt ttagataatt tatctagaaa tgcttgtcga tcttgctcta attgttgatc
    10981 atctacgcta ttgtctttag ccgaatcttg ttcactaggt ttatctctat tttctaacca
    11041 tttaggtgtt ttttcttttg aaatgcgatt acgctgccca tagtatgaac cacgcttttg
    11101 gtaatttccg ctagacccct catttttagg ttgattaact tttttagcgt aattatatgc
    11161 ttctttagct gtcttaatac cttttttctt ccaatttgat gctatttcca aaatatacgc
    11221 tttaggaagt ttcatatctt cttttaacat gacaaattgc aacaaaatat taatgacgcc
    11281 aaaagacatt ttttcacgtt caattaattc ttcaaccatt gtcttttgcg atatagttgg
    11341 ttctgattca gaccaagaag ctaacatatc aattggactc gtttgttcaa gtaattcaaa
    11401 ccattcatca ctttgtggca ttggattcgc ttctgtagat ttggccgtcg aagatgatgt
    11461 agcaggagat ttcacctgta atttaggcat ttgattttcg tgttcaatta agtaatacga
    11521 gcgtgcttgt ttacgcattt cttcaaagga taactgttgt ccacttgtaa ttgaatttaa
    11581 aataacatgc ttcatgccat ctgctgttaa accatataaa gtcgcgagtt gtgtaattaa
    11641 acgctttgca tctttggtaa caatgtcttg actaataaaa tgtttaccta acatttgtct
    11701 caacatttca aagtcaaaag attcatttga taaatcgata ccttggtacg gttcattaat
    11761 cggaatatca cttgtatcga tatctatttt tgtagacggc actttaaaaa catcagtgaa
    11821 ttgtctcgtt acctgtttaa attcactcaa atcaatttgt tgatactcaa agtatttctt
    11881 caactcatga aatcgacgat gctcgacttc actatataaa aagattgaca acattggatc
    11941 attaaaaaat aaatgtgctg aaggcggttg aattaattgg taaacaaatt gtgtttcttg
    12001 ttcatcatgt ttgacaaacg cctttaacaa tccaatcgct tcaagtaagt ccatttgttg
    12061 tctaaactct agtaaattaa ttttaagttc attcataaaa atataatgag aaagaatcaa
    12121 tgtttcatta tgactttctt taacgaattg agtcataaaa tggtataaac ccactgcttg
    12181 tgttccaatt agcggtgtat acagtcgatt caatacctct aaatgattcg tatttaaatc
    12241 aaagtgttgc ataactttga attgatcctt tggtcttaag ccgaattcga aggcttgtcg
    12301 tcccatttaa gcatcactcc gtttgttttc gcttaaaatc ccttgcatcg atgctaacaa
    12361 ttgatcaaca tctttaaatt ctttatagac tgatgcaaat ctaacatatg aaacttgatc
    12421 aacatgcatt aacaagttca taacgtgttc acctatatct cgtgaagaca cttccgtatg
    12481 accttcatct cgtaattgcc attcaacctt gtttgttatg tcttcaagtt gttgatatct
    12541 aactggtcgt ttctcacaag aacgcacaag tccattaagt attttttctc ttgaaaactg
    12601 ctctcttgtg ccatcttttt tcacaactat aagctgacta acttcgatat gttcaaatgt
    12661 agtgaaacgt gttccacaat tttcacattc tcttcgtctt cgaatggcat ttaattcatc
    12721 ggcatgcctt gaatctacaa ctttagattg tgtagaatta catttcgggc atttcattac
    12781 atcaccctct ttattttgat tatgcctaat tatactataa atctagagat gaaaaaagaa
    12841 tccctcaatt taattcattt aaccaaataa tgaaacaata aaaaacatta tatcgttact
    12901 tattaagtaa taagcatgac aatattattg tattaaaaat aaaagaccta actccgaggt
    12961 cagagttagg ctataaatta attgtattaa cttgcactta cagtttcttt tgatgtcaaa
    13021 agtgctccaa tttgctcagc gacatctaca actctatttg aataacccca ttcattatca
    13081 taccaagcaa taacttttac tttattctcc gacataacca ttgttgattt tgcatcaata
    13141 atagctgaat gtggattagt attaaaatca acagacacta gtggttgatg ttcgacttca
    13201 atgatacctt ctaaacctgc attttcaaaa gcttggttta cttcttctgc agttacttct
    13261 ttttctaaat caacaactaa atcaacaagc gatacattct ttgttggtac acgtaatgcc
    13321 atgccgtgta atttaccttc taattctggt aatacttctt ttaaagcttt cgccgcacca
    13381 gtagaagtag gaataatact ttcattacat gagcgtgcac gtcttaaatc tttatgtgga
    13441 ttatcaatat ttttttggtc atttgtaata gcgtgaacag tagtcattaa accattaact
    13501 attccaaact gattatttaa aacttttgca actggaccaa tgcaattagt agtacatgaa
    13561 gcattactaa aaatgtcaaa tgcttctata tctaattggt catcatttac gcctttaact
    13621 accatttgaa catgtccacc ttttgaagga ccagttaaca atactttttt ggcacctgct
    13681 ttaatatgtg cgatggcttt atcaccatga ttaaatttac cagttgcatc tatagcaata
    13741 tcgatatcta attctttcca tggcaagttt tcaggattgc gatcagcaac caatttaatt
    13801 ttatgatcac caacttgcaa tccattttca atcggttcaa cttttagatt atattttcca
    13861 tgtgttgtat cgtaattgat taaatgtgca attgtttcgg gtggataact agcatttatc
    13921 gctactacat ttaaattttt attttgtaat gcaatacgta ataccattct tccaattcta
    13981 cccataccat taattgcaat attcgttgac attaaaagca ccccttgatt tttatgtgtt
    14041 atactttatg gaattagtat agcatattat atgaaatttg aaagcgtttt ccgttgattt
    14101 atttatcttt tttataaaaa atagtcatta cttacataca tcaacgcata cgtaaagtaa
    14161 tgacttattt atagagtaat attaatcttc ttctccgtaa ttcggctttt caatataacc
    14221 ttcttcttct aacaatctct caaggttttg ttttaattca agtttatcac ctaaattatc
    14281 gataacatga tcggccattc ggcttttttt atcaatagaa atttggctat agacacgagc
    14341 ttttgcatct tctaatgaca aattattacg ttgcattaaa cgatccattt gtatactttc
    14401 agaagtgtat acaacccaca cttcgtctac tgtattttcc aattcatttt caaataataa
    14461 tggaatatcc atgattacat tatatccttg ttttaaatat tcttgctttt cttcttccat
    14521 aatatctcgc acgataggat gtatgatagc atttaattct aagcgttttt ctggatggtt
    14581 aaacactaaa tcacccatat aacgacgatt catctcacca ttttcatcaa ttgcttcatc
    14641 accaaagact tctcgtactt gagctaaacc tttgctcccc tttttaacag cttccctggc
    14701 tgctttatca gcatctacta ctttaaaacc gaatacggat aagagttctg atactgttga
    14761 ttttcctgag gcgattccac ctgttagacc aataactttc ggcataattt cactctttct
    14821 ttatttttga catactggac aataatgact atttcttgtc gcgatgattt ttgtttcaat
    14881 ttgacttcca cacactttgc ataccggctg cttatataca ttgaggtgca attgcatctc
    14941 accagttttt ccatcagcat gacgataatc tgaaatactt gtaccgccat gcttaatacc
    15001 ttcttctagt acttctctaa cataataaaa aaccatttct tgttgttggt gtgttaggtc
    15061 ttttactttt ttatctggta aaacacctgc acgaaacaac gcttcacatg cgtaaatatt
    15121 tccgcaacct gcgattactt tatgatccaa aatcacttgt ttgattggtt tattcttatt
    15181 agactgttga tgaattcgat ttaaataata cgtcaatgct tcatttgtaa aaggttcagg
    15241 cgctatttct ataaatgaag gataagatgc tacagacgca acatttctaa tttctccaaa
    15301 acgacgtata tctgaataaa ttaacttttt gtcatttgac agctcaaaaa taacatgcca
    15361 atgcttacga taattaggta tcataatatc ttcaagttca tctacaatga aaaaaccacc
    15421 cgccatacct aaatggctaa ttaatgtacg ttgttctcgt ttattatcta gctgaaaaac
    15481 gatatattta cttcttcgtt ctacattagt aatggtatag ccttccgata aagttttaaa
    15541 agtatctaat tcaattcctt ttataattgt ttccttgcct tgagctttac cttcaattac
    15601 tttatcagaa aatataacgt gttcaatttt ttgatttata acgtagggtt caattcctct
    15661 ttttacatgt tctacttctg gtaattcggg cataccatta acctcacttt attttgcatc
    15721 ataccaagtt gcaccataac ttgagtctac ttttaatgga acatctaatt gcaatgcatt
    15781 ttccattatc tcttctacaa attcactaaa tgaatctact tctgacttag gtacttcaaa
    15841 aattaattca tcgtgtactt gtaataatag tttagcttga tatgttgtct ctttcatttt
    15901 ttgcgcaaat ttaaccattg ccagtttaat gatatctgca gcactgcctt gtattggcgt
    15961 attcatagca gtacgttcag caaagccgcg taaattaaag ttacgactcg taatatcagg
    16021 aatatagcgt cgacgatgta gcaatgtttc cacgtaacct aaagctttgg catcttttac
    16081 aatatcagac atatattgtt ttacacctgg gaaactagct aaataatcat caatgaatac
    16141 ttttgctttt ttacgagtaa tacctaaact ttgacttaaa ccataatcac ttatcccata
    16201 aacaattcca aagttaaccg cttttgcttg acgacgcatt aaactatcga cttgatcagc
    16261 ttctacacca aatactttca tagcagttgc tgtatgaata tcatcgccgt tgataaatgc
    16321 ttctttcata ctctcatctt gtgtaatgtg tgctaataca cgtaattcaa tttgagaata
    16381 atctgctgat aatataacgc tatcttttga agttggttta aaggcttttc ttattttacg
    16441 cccttcttca agtctaaccg gaatattttg taaattagga tctacacttg ataaacgtcc
    16501 agtttgcgct aaagtttggt taaaacgtgt atggatacgt tgatcatcac taattacctt
    16561 ttgcaatcct tcaacataag tagactgtaa cttagataat tgacgatatt ctaaaatata
    16621 atcaatgata ggatgttcac cttgcaattg ctctaagaca tctacagcag tagaatatcc
    16681 tgttttcgtc tttttaataa caggtaattg taatgtctca aacaatacaa cacctaattg
    16741 cttaggagaa tttatattaa aatcttcacc agctgcatca tggatatttc gaatcaagac
    16801 gtctaatttt tcttgaattt ctttttccat ttcttctaaa tcatgaacat ctgtatatat
    16861 accaatttct tccatttcac ttaaaatctt agctagcggt agctctaaat cagctaagag
    16921 ttctacctga ttgtattctt ctaattgttt atccatattt ggtttcgcaa agtaaattgc
    16981 atcagtaata aaagcaacat atggatttaa aacatcatct tcaggtacct taaatttctt
    17041 accttttcca tatatactca catcgtcttt cacaaaactt tgaccgtaca atgaaacaac
    17101 tgattgaaca tcactaatcg tacgagatgg atcaattata taactggcca acataatatc
    17161 gaatgaaata ttttgaatat caattcccaa tctatgtgat gctacatatg tttttttagc
    17221 atcatatacg acttttttcg tattcggatt ttctaaccat gaaactagtt cggcataatt
    17281 atttatgtca tccgcattaa ttacaatatg tttctcacct gtaaataaag agaattttaa
    17341 aatattattt cgcaaataat tgccaccgtc taattcgaaa tggatggccg cttcttccaa
    17401 tgaagtaaaa tcaacattat caaaagacgt ttcaatttca aatgtctttt ctattgcatc
    17461 ttcaccgctt gctgattgat caatgtcagc caacaattgt ttgaattcta acttcttaaa
    17521 caattcgatt ttttcttgtt gttcatcttg atgagtcatt aacgtatctt caagttttac
    17581 ttcaatcgga ctatctacat taatcgttgc taattcttta ctcattaatg catcttcttt
    17641 gctgttttga agtttttctt ttaacttttt acctgaaatt tcatctaaat gttcatagac
    17701 accttctact gtgtcaaatt ggttcagcaa ttttattgct gttttctctc caacacctgc
    17761 aacacctggt atattatcag aagtatctcc cattaaacct ttcatatcaa taatttgatt
    17821 aggtgttaac ccgttgtatt tttccgcaat aaagtcaggt gtataatgat caacatcagt
    17881 aacacctttt ttagtgtaat aaatggttac attatccgtt gcaagttgtg ttaaatctcg
    17941 gtctcccgta ataataattg tctgaaagcc cgctttatct gcttctttac ttaaagttcc
    18001 gataatatca tctgcctcat agttatctaa ttcataacgt ttaatatgat aagcatctaa
    18061 taattggcgt atataaggaa attgctcact tagttcaggc ggcgttttct ggcgtccacc
    18121 tttatattca ctatattttt catgtctgaa agtcgtttta cctgcatcaa acgctactaa
    18181 aaaatgattt ggcttttctt cttttaaaat cttctctagt aacattgcaa aaccatatac
    18241 tgcattggta tgaatgcctg ctttgtttga taacaaaggt aatgcataaa aagctctaaa
    18301 acttaagcta ttaccatcga ttaatactaa tttattcaca attttaacct ccagaactaa
    18361 tttatatata ttttagcata ttcgactgtg atgtcgtaat taaagaaact atctcataga
    18421 gtatttatat acgacctaat ttcaaataaa acaagtgact atatagacta agctaaaata
    18481 aagaagacat aatcatgatt gttcttgatg agcacttcat aaagtcaggt taaaagtcca
    18541 acttttgaga tgcacattat catttcatga ttatgtctac tttttaatat ttttatgctg
    18601 ctattttaaa tttttatagg taagaataag ttatgaatcg tcagaaccaa atggaatata
    18661 tttaactttg gtcattttcg gtaattcttc ataattatta aaccactctt ggtaattttt
    18721 attgattggt gacggatgat gcatataatg ttcaaacggc aatgattcca ctgtataaac
    18781 ctcttgtcgt ggatgatctt taatttgttg ttttaataac tgaactcttt tttcatgttc
    18841 ataatgaaca taaataaatg cactaagata tatcatagct aaaactagtg atgcaccttt
    18901 aataaaatta acattgattg acttatattt ccgaaattct tttaataaaa taattaaaat
    18961 gattacatgt atcgtataaa caatcaaaaa attacctggt tcaattggag taacaatgac
    19021 taatgtcgac gcagaaacac atattgcaat aagtaaggaa tataaggtaa tttgtgtttt
    19081 acgattatta atagacaaat aaatacctac aaatatcgaa aacgcaaagt aaccacatac
    19141 aattatgttc gcaaaaccaa ccaatccaat atctgtattt ttatttaata aaaactgatt
    19201 gtaaaatagt aaataataaa gcggtaaagt gataaatcct atcataatga tacgacgctt
    19261 taatattgtt aaatgtacat accgatcact tctaagcagt agataaataa taagcgttgc
    19321 tatcatactt aaaattataa cctgactgaa aatgacacca tatggaagtg ttgttgatat
    19381 catttctgca aattttgaaa agataccttg attattagaa acttgttgat attctgatcc
    19441 ttcaaataaa attttgcgat aatttggatt ggaaaacata atgattgtac ctatagtagc
    19501 aatcataaat gaaaacaata atttataatt aagtgttcga ttggcagtaa attcatacag
    19561 aaaagctaaa attaaaatga tacagtggaa taatgtcaca ttctccataa ataattgacc
    19621 gaagaagctt aaaacataga atagtactgt aacacttacc ggctgctttt ctttatatat
    19681 aattgcatta atacaataat aaatgataaa gagtgaaatt agtgttgatg tcgcataatt
    19741 gtaaaatcct gcaaaccagc catatgtatc tgcataaata gcacttggta aaattaacat
    19801 caaagaaaat gctaacaaat aatagcttgt ccacgcttta caacgtgtaa tgtgcataat
    19861 catccaaatg atacccatac taatgagacc atatgaaagc caacgtaacc agcttacatg
    19921 tacagctata atttcaaaga tatttccgat atagcgacca ttaagtgatg caaatcctac
    19981 ttttaaaata tcagtattat aattgctaaa ccattgtaaa tcatcgtgca tgagtggtag
    20041 taagataccc ataaaagtat aaaacaataa tatcgcaatt aatatcaaag ttgtcttgtg
    20101 taattgaatt gttttcactt tactaatcct caaatctagt taaattttcc tcaacttgta
    20161 ggtcgaaaaa ttaattcaat atttttaatg tatttctaat tttcacctat gcatgtttcc
    20221 tcaatcaaaa tagataaaca aggtatttaa tattactttt caacaattta tctaaatcgc
    20281 ccctcgtctt tttctatgac gaatgattac acttgtgtat tcaagtgttt aaattaagat
    20341 ttatttcctt tcaaataaaa cttattactt tttaattaag tatctatttt aagcgcgtta
    20401 ctaaaatttc tttattataa t
//
LOCUS       DAPIDM010000036        18926 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000036 DAPIDM010000000
VERSION     DAPIDM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.036"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..217
                     /locus_tag="RJE42_001153"
     CDS             <1..217
                     /locus_tag="RJE42_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV5876584.1"
                     /translation="MIEHQQIDALTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTI
                     KSYQQPVTIAEIQTLESLIEKILESRG"
     gene            220..1194
                     /gene="hemB"
                     /locus_tag="RJE42_001154"
     CDS             220..1194
                     /gene="hemB"
                     /locus_tag="RJE42_001154"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV5876585.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGTDMIITYFAKDICRYLDK"
     gene            1242..2528
                     /gene="hemL"
                     /locus_tag="RJE42_001155"
     CDS             1242..2528
                     /gene="hemL"
                     /locus_tag="RJE42_001155"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV5876586.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITSEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            2589..2690
                     /locus_tag="RJE42_001156"
                     /pseudo
     CDS             2589..2690
                     /locus_tag="RJE42_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2724..3797
                     /locus_tag="RJE42_001157"
     CDS             2724..3797
                     /locus_tag="RJE42_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV5876587.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHVNGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(4020..4580)
                     /locus_tag="RJE42_001158"
     CDS             complement(4020..4580)
                     /locus_tag="RJE42_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV5876588.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAFGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPRHN"
     misc_binding    4638..4939
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4983..7613
                     /locus_tag="RJE42_001159"
     CDS             4983..7613
                     /locus_tag="RJE42_001159"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV5876589.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNVLVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHQGETIVKASWPEVRESLVFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            7626..8897
                     /locus_tag="RJE42_001160"
     CDS             7626..8897
                     /locus_tag="RJE42_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV5876590.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPI
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKYLSLIAHTFYATEFDFPKALRKEEIVGSIENDDIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            9159..9866
                     /locus_tag="RJE42_001161"
     CDS             9159..9866
                     /locus_tag="RJE42_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV5876591.1"
                     /translation="MVVLLSYICSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCHKRISLTHFFGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSIIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVIIFTFVIAGLVALITLIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            9863..10549
                     /gene="radC"
                     /locus_tag="RJE42_001162"
     CDS             9863..10549
                     /gene="radC"
                     /locus_tag="RJE42_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV5876592.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIQVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            10617..10901
                     /locus_tag="RJE42_001163"
     CDS             10617..10901
                     /locus_tag="RJE42_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876593.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(11018..11215)
                     /locus_tag="RJE42_001164"
     CDS             complement(11018..11215)
                     /locus_tag="RJE42_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876594.1"
                     /translation="MSKLTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            11375..11848
                     /locus_tag="RJE42_001165"
     CDS             11375..11848
                     /locus_tag="RJE42_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDV5876595.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            11922..12041
                     /locus_tag="RJE42_001166"
     CDS             11922..12041
                     /locus_tag="RJE42_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876596.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNR"
     gene            12239..13081
                     /gene="mreC"
                     /locus_tag="RJE42_001167"
     CDS             12239..13081
                     /gene="mreC"
                     /locus_tag="RJE42_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV5876597.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKEAKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSKGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            13081..13611
                     /gene="mreD"
                     /locus_tag="RJE42_001168"
     CDS             13081..13611
                     /gene="mreD"
                     /locus_tag="RJE42_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV5876598.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRSFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    13638..13833
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            13846..14154
                     /gene="rplU"
                     /locus_tag="RJE42_001169"
     CDS             13846..14154
                     /gene="rplU"
                     /locus_tag="RJE42_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV5876599.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            14160..14480
                     /locus_tag="RJE42_001170"
     CDS             14160..14480
                     /locus_tag="RJE42_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDV5876600.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            14492..14776
                     /gene="rpmA"
                     /locus_tag="RJE42_001171"
     CDS             14492..14776
                     /gene="rpmA"
                     /locus_tag="RJE42_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV5876601.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            15263..16555
                     /gene="obgE"
                     /locus_tag="RJE42_001172"
     CDS             15263..16555
                     /gene="obgE"
                     /locus_tag="RJE42_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDV5876602.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVDTDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLILFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            16568..17023
                     /locus_tag="RJE42_001173"
     CDS             16568..17023
                     /locus_tag="RJE42_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDV5876603.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            17037..17639
                     /gene="ruvA"
                     /locus_tag="RJE42_001174"
     CDS             17037..17639
                     /gene="ruvA"
                     /locus_tag="RJE42_001174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV5876604.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            17671..18675
                     /gene="ruvB"
                     /locus_tag="RJE42_001175"
     CDS             17671..18675
                     /gene="ruvB"
                     /locus_tag="RJE42_001175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV5876605.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDAIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            18677..>18926
                     /locus_tag="RJE42_001176"
     CDS             18677..>18926
                     /locus_tag="RJE42_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HDV5876606.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLM"
BASE COUNT         6594 a         2570 c         3669 g         6093 t
ORIGIN      
        1 aatgatagaa catcaacaaa tcgatgcatt aacattttca agttcgtcgg cagtacgtta
       61 ttattttaat gaaggatttg taccaaaatt caagtcgtat tttgctattg gagaacaaac
      121 agcacggacc attaaatcat atcaacaacc agtaacaatt gcagaaattc aaacactcga
      181 atcactaatt gaaaagattt tagaaagtag gggctaaaaa tgaaatttga tagacataga
      241 agattgagat catcagcgac aatgagagat atggttagag aaaatcatgt aagaaaagaa
      301 gatttaatat atccaatttt tgtagttgaa aaagacgatg tgaaaaaaga aattaagtca
      361 ttgccaggtg tataccaaat cagtttgaat ttacttgaaa gtgaattaaa agaagcttat
      421 gacttaggca tacgtgccat tatgtttttc ggtgttccaa actcaaaaga tgatataggt
      481 actggtgcat acattcacga tggtgttatt caacaggcaa cacgtattgc taaaaaaatg
      541 tatgatgact tattaattgt tgcagacact tgtttatgtg aatatactga tcatggtcat
      601 tgtggtgtga ttgatgacca tacacatgac gttgacaatg ataaatcttt gccactactt
      661 gttaaaacag caatttctca agtagaagct ggtgctgata ttattgcacc aagtaatatg
      721 atggatggtt ttgttgctga aattcgtcgt ggattagatg aagccggcta ttacaatatt
      781 cctataatga gttatggtgt caagtatgca tcaagtttct tcggaccttt tagagatgca
      841 gcagattcag cgccatcatt tggggataga aaaacgtatc agatggaccc tgctaaccgt
      901 ttggaagcac ttcgtgaatt agaaagtgat cttaaagaag ggtgcgacat gatgattgtt
      961 aaacctgctc taagttattt agacatagtt cgagacgtta aaaatcatac gaatgttcca
     1021 gttgttgcat ataatgtgag tggagaatat agtatgacta aagcagcggc acaaaatggt
     1081 tggatagatg aagaacgtgt cgttatggaa caaatggttt caatgaaacg tgcaggtact
     1141 gatatgatta ttacgtattt tgcaaaggac atttgtcgct atttagataa ataaggtttt
     1201 atatgtatga ttttccataa actataggag gaatttactt tatgagatat acgaagtcag
     1261 aagaagcaat gaaggttgct gaaactttaa tgcctggtgg tgtaaatagt ccagtacgcg
     1321 catttaaatc agtagataca ccagcaattt ttatggatca cggtaaaggc tcaaaaattt
     1381 atgatatcga tggtaacgag tatatcgact atgtactaag ttgggggcca cttattttag
     1441 gacatagaga ccctcaagtt attagtcatt tacatgaagc aattgataaa ggtacaagtt
     1501 ttggtgcatc aacattactt gaaaataaat tggcgcagct cgttattgat cgagtacctt
     1561 caatagaaaa agtacgtatg gtgtcatctg gtacagaagc tacattagat actttaagat
     1621 tagcacgagg ttatactggc agaaataaaa ttgtgaaatt tgaaggttgc tatcatggtc
     1681 atagtgattc gttattaatc aaagctgggt ctggagtggc aacattagga ttgccagatt
     1741 ctcctggtgt gcctgaaggt attgctaaaa atacaattac agttccatac aatgatttag
     1801 atgcacttaa aatcgctttt gaaaaatttg gagacgatat tgctggtgta atcgtagaac
     1861 ctgttgctgg taatatgggt gtcgtaccgc cgattgaagg ttttttacag ggattaagag
     1921 atattacgtc tgaatacggc gcattgctaa ttttcgatga agtaatgact ggtttcagag
     1981 tcggttatca ttgtgcacaa ggtcactttg gtgtgacacc agatttaact tgcttaggaa
     2041 aagttatcgg tggaggacta cctgtaggtg cttttggtgg taaaaaagaa atcatggatc
     2101 atatagcacc attaggaaat atttatcaag cgggtacgtt atcaggaaat cctcttgcaa
     2161 tgacaagtgg ttatgaaaca ttaagtcaat taacgccaga gacatatgag tattttaata
     2221 tgttaggcga tatacttgaa gacggtttaa agcgtgtatt tgctaaacac aatgtaccaa
     2281 taactgtaaa tagagcaggt tcaatgattg gttatttctt aaatgaagga cctgtaacta
     2341 attttgaaca agcgaataaa agtgatttga aattatttgc agaaatgtat cgtgaaatgg
     2401 caaaagaagg tgtgttttta ccaccatctc aatttgaagg tacattctta tctacggcac
     2461 acacgaaaga agatattgaa aaaacaattc aagcatttga tacggcttta agtcgtattg
     2521 taaaataaat atacggacaa attgagagcc tgaactttgt tcaggctctt tttaaatgta
     2581 tatcaggcat ggactacgac ttgatagtga aagtccatat atgatttgta gtcaaaactg
     2641 ctagcggata ttgttatctt aacaaacgtg aagctcaagc agcaatttag tcattttatt
     2701 ttttattgaa agaagtggaa aacatgacaa tgatatatag aaataatttc attgtgttcg
     2761 ttttatcatt ttttattagt attctactgt attcatcgca cgttttactc ccatttatgt
     2821 ttggtcctat tatcgcatca atcatttgtg taaaagtttt caaacttgat attaaatggc
     2881 cattcttact tagtgaatta ggtattgtac tattaggtgt gcaaatcgga tcaacgttta
     2941 cgaaaaatgt tgttatggat attaaagaca attggctttc gattattgtt gtatctattt
     3001 cgatattatt aattgcctta gtaatggcat ttattttcaa aaaaattgca cgtattaata
     3061 cagaaacagc tattttaagt gttataccag gagcactaac acaaatgttg gtcatggctg
     3121 aacaagacaa acgtgctaat ttgttagttg ttagcttaac gcaaacatca cgaattatat
     3181 ttgttgttgt tttagtaccg ttcatttcat atttttttca tgatggtaac atgcatgtga
     3241 atggtaagtt aacaaaagtc ttgcctttat cacaagtatt aaacataggg caaatagtta
     3301 ttttagcgat agctatattt atagtttatc taattatgtc taaaataaag tttccaacat
     3361 ttcaattatt agcaccactc attgtattaa ttgtttggaa tttttctaca ggtctaacat
     3421 ttacactaga tcattggttg ttgaacatgg cacaactaat atatatgatt agaattggag
     3481 ttcaaattgc gcatttattg tcagatttaa aaggtagact agcaatcgca attacaattc
     3541 aaaatattat gttgataatt ggtgcgctaa tcatggttta tgtcatacat ttctttgata
     3601 ataatcctat taatgaattg tttcttggtg cagcgccagg aggggttaat cagatagttt
     3661 tagttgcatt agctactggt gccgatgttg ctatgattac gagttatcat attttccgaa
     3721 tctttttcat cctatttatt attgcaccag gaattaatta ctttttaaaa tatcgttcga
     3781 ataagcgtga agattagaaa ctatcatcca tttaagttaa attatgctta ggatagaatt
     3841 cattattaag gtttaagcaa aggttttaca tctattttta aattaagata tctaatatta
     3901 gcagtgcatt aacatggaac tttaacttgt gatttgaata aatttctaca ttaaaaaacg
     3961 agacagtcta ttagttaaaa atagatgatg tctcgtttat tttatgatgc tatagcaaat
     4021 taattgtgtc taggctttga tggacaatct tttaaatgtg catcatataa accggctgct
     4081 tctaaaaacg aaaatactgt tactggacct aaaaatttga acccgtattg ttttaaatct
     4141 ttagatagtt gtgttgcagt atcatcaact gtgatacgat cagaagcatg ttcatactgc
     4201 aaatctttag gcttaccatt tacataagac cataaaaatt tactaaaact accaaatgct
     4261 tgttcaattt ttaaataccc ttgagcttga ttaacaattg cttctaattt tttacgatga
     4321 tgaacgatat ttggaaaagt cattaagcgg tcgatatctt gagcggtcat ttgtgctacc
     4381 ttttctggtt cgaaatcata aaatgcttct tcataggctt ctttcttttt taaaatagtt
     4441 aaccaagata gcccagcatg ttgtgattct aatgctaaaa gtttaaacaa tgccttgcta
     4501 tcatagagcg gttgtcccca tacatgatca tgatagtcta agtagactgg atctttagtt
     4561 ccaaatgcgc attcattcat tttattccca cctatacatt gacttatttt gtcaatcatt
     4621 atactataag ttaagagttt aatataggat ttgggaagag taagttcaaa ttattttaaa
     4681 gagagcatat gatttggtgt gagtatgtaa atgattttta ctgaaggtag cccacagagt
     4741 atcttagctg aacgtgatta atagtaggct aagacgtgtt tgagcgttaa tcataatcta
     4801 gagtgcttga agagatgaag catttcaaat ttcacgatga tagcattggt atgattattc
     4861 caattatgtt aaaagagtga atttgatatt aatcatcctt cttgaatata ggtggtacca
     4921 cggaatatcc gtcctatttg tatataggat ggatattttt tattttttag gaggtatagc
     4981 aaatggaaat gaaaccaaaa tatgatcctc gtgaagttga agcgggacgt tatgaagaat
     5041 gggtaaagaa tggttatttt aaaccgtcag aagataaatc aaaagaaaca tatacaattg
     5101 ttatcccgcc accaaatgta actggtaaat tacatttagg acatgcatgg gatacaactt
     5161 tacaagatat cattacacgt atgaaacgta tgcaaggata cgatacgtta tacttaccag
     5221 gtatggatca tgctggtatt gcgacacagg caaaggtaga agctaaatta aatgaacaag
     5281 gaataactag atatgatctt ggtcgtgaaa agtttttaga acaggcatgg gattggaaag
     5341 aagagtatgc gtcatttatt cgtgcgcaat gggctaaatt aggtctaggt ttagattata
     5401 gtagagaacg ttttacttta gatgaaggtt taagtaaagc agttaaaaaa gtttttgttg
     5461 atttatacaa taaaggaatt atttatcgtg gcgaacgtat tataaattgg gatcctaaag
     5521 cacgtacagc tttatctgat attgaagtaa tacatgaaga tgttcaaggt gcgttttatc
     5581 attttaaata tccttacgct gatggtgaag gttttattga aattgcaaca acaagaccag
     5641 aaacgatgtt aggtgataca gcgattgttg ttaaccctaa tgacgaacga tacaaagatg
     5701 taatcggtaa aactgttata ttaccaatcg taggacgcga actgcctatt ttagcagatg
     5761 agtatgtcga tatagacttc ggttctggtg ctatgaaagt gacaccagca catgacccta
     5821 atgattttga aattggtcaa agacatcaat tagaaaatat tatcgttatg gatgaaaatg
     5881 gtaaaatgaa cgacaaagct ggtaaatacg aaggtatgga ccgttttgat tgtcgtaaac
     5941 agctagttga agatttaaaa gaacaagatt tagttatcaa gattgaagat catgttcatt
     6001 ctgtaggtca ttcagaacga tctggcgctg ttgttgaacc atatttatca acgcaatggt
     6061 ttgtgcgcat ggaagactta gcgaaacgtt cattagataa ccaaaaaaca gatgatcgta
     6121 ttgattttta tccgcaacgt ttcgaacata catttaacca atggatggaa aatattagag
     6181 attggacgat ttcaagacaa ttatggtggg gtcatcaaat tccggcttgg tatcataaag
     6241 aaacaggcga aatatatgtt ggagaagaag cgccaactga tattgaaaat tggcaacaag
     6301 atgaagatgt attagatacg tggttctcaa gtgctttatg gcctttctct acgttaggtt
     6361 ggcctgattt agaaagcgaa gactttaaac gatactaccc aacaaatgtc ttagttacag
     6421 gttacgacat tatctttttc tgggtagcac gtatgatatt ccaaggctta gaatttacag
     6481 atcgtcgtcc atttaatgat gtgttattac acggcttagt tcgcgctgaa gacgggcgta
     6541 agatgagtaa atcattaggt aatggtgtgg atccaatgga tgttattgac gaatacggtg
     6601 ctgatagctt gcgttacttc ttagcaacgg gttcatctcc aggacatgat ttaagatact
     6661 caactgaaaa agttgagtca gtgtggaact ttatcaataa aatttggaat ggagcacgtt
     6721 tcagtttaat gaatatcggt gaagacttta aagttgaaga tatcgattta agtggtaact
     6781 tatcattagc agataaatgg attctaacac gtttaaatga aacgattgca acagttactg
     6841 atctaagtga caaatatgaa ttcggtgaag ttggacgagc attatataat ttcatttggg
     6901 atgatttctg tgattggtac attgaaatga gtaaaattcc aatgaatggt aatgatgaag
     6961 aacaaaaaca agttacccgt tcagtattga gttatacttt agacaatatt atgagaatgc
     7021 tacatccatt catgccattt gtaacagaga aaatatggca aagtttacca catcaaggtg
     7081 aaacaattgt taaagcttca tggccagaag tgcgtgaatc attggttttt gaagaaagta
     7141 aacaaacaat gcaacaactt gttgaaatca ttaaatctgt aagacaatca cgtgtagaag
     7201 taaatacgcc attgtctaaa gaaataccta ttttaattca agctaaagat aaagaaattg
     7261 aaacaacttt atcacaaaac aaagattatt taatcaaatt ctgtaatcct agtacgttaa
     7321 atataagcac tgacgtggaa attcctgaga aagcaatgac atcagttgta attgcaggta
     7381 aagtggtatt accattagaa gggctaattg atatggataa ggaaatcagc cgtttggaaa
     7441 aagaattagc taaacttcaa agcgaattag atagagtaga taaaaagctc tctaatgaaa
     7501 actttgtaag taaagcacct gaaaaggtta taaatgaaga aaaacgtaaa aaacaagatt
     7561 atcaagaaaa atatgatggt gtgaaggcaa gaattgaaca attaaaagca taggagttag
     7621 taacaatgaa ttacctagag agcttgtatt ggatacacga aagaactaaa tttggcatca
     7681 aaccaggtgt taaacgtatg gaatggatgc tagcacaatt taataatcct caaaataaca
     7741 ttaagggtat tcatgtaggt ggcacgaatg gtaaaggctc tacagttgct taccttagaa
     7801 cagctttagt tgaaaatggt tatgaagtag gtacatttac gtcgccgttt attgaaacat
     7861 ttaatgaacg aattagtcta aatggtgtgc caatatcaaa tgacgctatt gtagaattag
     7921 tatctcgtat taaaccagtt agtgaaatga tggaacgtga aacagattta ggtgttgcaa
     7981 ctgaattcga aataatcaca gcgatgatgt ttttatattt tggtgaaata catcctgttg
     8041 attttgtcat tgttgaggct ggattgggta taaagaacga ttcgacaaat gtctttacac
     8101 cgattttatc aatcttaact agtatcggtc tagaccatac agatatttta ggtggtactt
     8161 atctagatat tgctagggat aaaggcgcga ttataaagcc taacgttcca gtgatatatg
     8221 ctgttaaaaa tgaagatgca ttaaagtatg ttcgtgaacg cgcaattgaa caacatgcaa
     8281 agccaattga attagataga gaaattgttg ttgtatcgca aaatgatgaa tttacttacc
     8341 gttataaaga ttatgaatta gaaacaatca ttttaagtat gttaggtgaa catcagaaac
     8401 aaaatgctgc attagccata acagctctta ttgaattaaa tgaacaagga ttaattgaat
     8461 tagatttcaa taagatgata gacggtattg aatcagttcg ttggactgga cgtattgagc
     8521 aggtgcatga caaaccttta atcattttgg atggcgcaca taattcagag agtatagatg
     8581 ctctaattga tacaattaaa cagtaccatg ataaagaaaa agtagatatt ttgttctcag
     8641 caataaacgg aaaaccgatt aacgagatgg tcaaatattt aagtttaatt gcgcatacat
     8701 tttatgcaac tgaatttgat tttccgaaag cgttacgcaa agaagaaatt gtaggtagta
     8761 ttgaaaatga cgatatacaa ttagtagatg actacgtaga atttataaaa aattatcaag
     8821 gtgatacatt agtaattacc ggtagtctgt acttcataag tgaagttaaa tcaaaattag
     8881 actttgaagc attataaaac taaaatagcc atgaagaatc aatttaatca ataggtagct
     8941 tcatggttat tttttatgct ctaaatcgta tgtgtttata aggtttaatt tcttgccatt
     9001 attaaatttg ccgaacagta atgggctgtt aaagtatcca tcaattatgc ttagtaatat
     9061 tgaaaaattt aaatgaaaaa gaggttcaat atttggagaa tggtgtatat ttacattttg
     9121 gtaataagta actataattt acgtaaggag gttgtcattt ggtagtattg ttatcttata
     9181 tttgcagttg tatttttagt tttctatatc aatttatatc tattgaagaa acgtcatttg
     9241 attatttaca tagaagatcg aaatgtgatt attgtaattc atcactcaaa tggtatgaat
     9301 taatgccgat tattagtttt ttattattaa aagggagatg tcgaaactgt cataagcgta
     9361 tttcactaac acatttcttt ggggaaacct ttgctttaat acctatcgtc tttattaagt
     9421 atgatttcac atacgtaaat gctacgcttt ttataactac atatgttttt ctgcttatat
     9481 ttactatgac cgatatcact tctttaatgt tagattgtcg cttaattata atttattgta
     9541 tcgtttctct ctcgttaagt attatttatc cagtagcttt tatcattatt agtatgacca
     9601 cgcatatatt ctacttttta tttcgggcat atattggtta tggtgacgtt ttactaatat
     9661 ctgcactttc tttgtttttc cctctccaat tcactattta tgtcattata tttacatttg
     9721 tcattgctgg tttagttgct ttaattacct tgatatttaa gccgattaaa ctattacccc
     9781 ttgttccatt tatatttatt tcctttttta tcaattcact tttttataat gatatccatc
     9841 aatttttagg aggcgtatat ttttgaaaat taaagaaatg gtaacttcag aaatgccaag
     9901 agaacgtttg ttaagccatg gtgcaaaaag tctttcgaat acagaattat tagctatatt
     9961 aattaacacc ggaagaaaag gattctcgag tattgacatt agtaatgaac tgcttaaatc
    10021 tgcatcgaat ctgaatgaat tgaaaaaatc ttcaattaac gatttgatac aagttaaagg
    10081 gattggatta caaaaagcaa ttacattaaa agcggcattt gagttagggg aaaggatggg
    10141 gagaagagct gaaaataatc gtataaaaat aacgcaacca agtgatgttg ctgattatat
    10201 gattccaaca atgaaagatt taacacaaga acattttgtc attttattgt taaattcaaa
    10261 aaatgtagtg attaaagaaa cctgtgtttt taaaggtaca ttaaatagtt cgattgtaca
    10321 tccacgtgaa atttttagta ttgcggtgag agaaaatgcc aatgcaatca tcgcagttca
    10381 taatcatcca tccggtgatg taacgccctc acaagaagat atcataacaa caatgaggtt
    10441 gaaggagtgt ggtttgattt tagggataga tttattggat catattataa tcggtgataa
    10501 tagatttacg agtcttgtag aagcgggtta ctttgatgaa aatgattgaa gttgatagaa
    10561 ttaatgacgt atcttgtgta taatacctac gaagtacttt cattggagga aaaatagtga
    10621 ctctatttat tattatcggg gttctcgtgc caatggttta taccatgcag ttaaatatta
    10681 aaaatgaacc tgtaacaaag cgcaatctat taataacatt agctttatct acgttaggta
    10741 ttttagtaac ggcattagca ggtgtgattg ttacgaaaca agcttttcct ttattaagtg
    10801 tagcaatagg ctcaattttt actggaatcg tttggggcct attactaagt ggtagctacg
    10861 cgctgatacg atttttatct aacgcatttg ggcgtaagta atagtaatct gttcatcaag
    10921 tagtaaacgt gcttggaaac aaattaaaac tcctaatgtg gaactagtta aaaaattcta
    10981 aactagtaca taaggagttt tgttatgcag aataaattta attgttaaat tgaaagtgtg
    11041 gtaaaaaccc actatttatt tgaaaaaaat cgagcaacca tattaaacct tgatatccta
    11101 agtaaataca taataaaaca agtccgacat aaattaaaaa tcgcaaaata gaaagtccaa
    11161 ctctaaaaag gaggatgact aatagtgcta ttaaaataat tgttagttta ctcaatgcta
    11221 caaacctcct catacgcttt taaatcataa caaaattata agctatttta aatgaacgtg
    11281 catcagtcct aagatatatc atgattaagg ctgaagactg aggaaaatat acataataaa
    11341 atatataatt agatgaagac attggaggcg aactatgaga ttgatattta gtatgattaa
    11401 aaacattata gcagtaatag caattgtgat aattgtttat atcgccttga aatacgcacc
    11461 ttttttaaaa gaacaagatt ggaatcctgt aaatcatgat aatgatcaaa tgaatcaagt
    11521 tacgcaaccc actaatgatg cccaacatgt ttatgtaagt ggtgagaaat attcattaaa
    11581 agaaaatgat ttaataaaga atgtaccatt aagccaaatt aaaaatgtat ttaaaatgat
    11641 agataaacaa gagtttatgg ctgtgtctgg aatgaatcgc atggcttata atgatcaata
    11701 tattataggt caaagaggag acgaatttat tctttataaa tttggagatg agtcaatgcg
    11761 tgtttacaat actgaatttg aaatgcaaca agacttaaat gaattagggc aaaatttaca
    11821 actaaaacct gaaaatgctt atcaatagga aattattgca taagaaatat attttagtta
    11881 tcctgtcact agaataaatt taaaaggttg gtgtagttca aatggcagaa caatcaaaac
    11941 agaaacaagc taatgaacaa caaaaggccc agaacttatt cgcacgttgg agacaacttc
    12001 agaattcgaa cagtgaatcc tcgaacgata caaacagata attttaaaat ttaaatcgat
    12061 tatttacact actagtgact agaatgtttg ctagtagttt tttgttttta aaatgccaca
    12121 aatttttacg gagttgctca ttttcaaagt aattcgtagt tagtgcttta tttcataatg
    12181 ggagtaatat aaaatagaaa aggatataca atattttgac ataatagagg tgttctgggt
    12241 gcttaagttt tttaaaaata acaaattaat tgttgtttta tgtgcaatta tcgtttttat
    12301 tgcattaatt gggctgtcca tacgttcaca atctcaatca cctcctgaac aatatatagg
    12361 tgattctgtg tcttttggac aacgagttgt gagttatcca gttaattttg ttgctggtac
    12421 gattggggac ttttttaaaa aaggagattc taaagaagct aaaaataaga ttagccagtt
    12481 agaatctaag aaccaacaat tagaagcgga aaatgaaaaa ttaaaaaaag agcttgattt
    12541 aaaagatatt tcaaaatttg atcctatttc tactacggtt ttagcaagaa atccggatca
    12601 gtggatgaat acaattgtaa ttgataaggg atctaaatca ggtataactt caaatatggc
    12661 tgtgatgaca tcaaaaggtt ttgttggaag agttactaaa gttaataaat tttcttcaca
    12721 agttgattta atctcaacta atacacgtgc gggtaaatta tctgtaaata tacaacacgg
    12781 ttctaaaaat atatttggct taattgatcg ttatgatgaa aagaactcag aacttgtaat
    12841 tagtgatatt aataatagag ataatatctc aaaaggagat aaagtcgtta caagtggatt
    12901 agctgatcaa ttaccaagta atttatatat tggagaagtg actaaggttc aaaatgatca
    12961 atacggctta gctaaagaag ttagggttaa gactggtgca gacttaacag atttgagcca
    13021 tgtttatgtt gcaaaaagag atcctaaaac aattcctgat gatgaaagca gggataaata
    13081 atgcgtacac tgtattattt tttgataggt attttactat tttatataga tactgcaatt
    13141 gggcttctta taccaatgca cataggtaag tttgaacttg tatttgtacc acaccttaca
    13201 tttatgtata ttttaatgat ggtagtgtat cgtagctttg gcgtatcatt attgctcagc
    13261 atatttttag gtgtaatgac tgatgtatac tttggtagta tttatggagt gtacttattt
    13321 ggctatatat tgtttttagc acttatagat cgattcttta aaatttttta caaagatcat
    13381 tcgatgttat tcattattat attagccagc accttattat tagaagtcta tgtggcatta
    13441 atatacggta tgttaggatt cattcaattt gatattattc attttgtagt ctttagatta
    13501 ttgccaacat taattatgaa ttttgtactg ctaatcatgc tttatccgtt gattataaag
    13561 ttccttaaaa aaacaaacaa tgacattgac atgaaacgcc gtcaatggta agatgcaata
    13621 gttgagtagt tataagtagc tacatacaac cgctcaaata taggttcaag aacattttta
    13681 atgaagaaag cgattgtcaa tcaaggcgtt gaaggaagaa acgagagagt ttatctatat
    13741 aaatgactcg agttgcgacc gaaacaacgt agagtgcaaa cgcttgactc aataacgaaa
    13801 tgtcacctat gtatggcgtg acttattata caggaggtgc aaagtatgtt tgctattatt
    13861 gaaacaggtg gaaaacaaat caaagtagaa gaaggtcaag aaatcttcgt tgaaaaatta
    13921 gacgtaaacg aaggagatac ttttacattt gataaagtat tatttgtagg tggagattca
    13981 gttaaagttg gagcgccaac agttgaaggt gcaacagtta ctgctactgt taataaacaa
    14041 ggtcgcggta aaaaaatcac tgtattcaca tacaaacgtc gtaaaaattc aaaacgtaaa
    14101 aaaggccatc gtcaaccata cactaaatta acaatcgata aaatcaacgc gtaattatta
    14161 tgattactgt tgatattaca gttaatgatg aaggcaaagt aacagacgtt attatggatg
    14221 gccatgctga ccatggtgaa tatggtcatg atatcgtttg tgctggagct tcagctgtat
    14281 tgtttggtag tgttaatgcg attataggat tgacatctga gagaccagat atcaattatg
    14341 acgacaatgg tggtcatttt catataagaa gcgttgatac aaataacgat gaagcgcaac
    14401 taattcttca aacaatgctt gtgtctttac aaactattga agaagaatat aatgagaata
    14461 ttagattaaa ttataagtga ggtgcattcc gatgttaaaa ttaaacttac aattcttcgc
    14521 atctaaaaaa ggggtaagtt ctacaaaaaa cggacgtgac tctgaatcaa aacgcttagg
    14581 tgctaaacgt gctgacggtc aattcgtaac aggtggttca attttatatc gccaacgtgg
    14641 tactaaaatt taccctggtg aaaatgtagg tcgtggtggc gatgatacat tattcgctaa
    14701 aatcgacggc gttgttaaat tcgaacgtaa aggtcgcgac aaaaaacaag tttctgtata
    14761 tgcagtagct gaataatttt gtctagttaa caccagaagt gaatcttctg gtgtttttta
    14821 ctttttataa aacgtttttc attattattt tcattttgtt attaaaaaat gggaattata
    14881 gacgttatac taaatgtgca actgcataag agcccctaat cgctaaacta taaggggaca
    14941 aaggattaca gttgcagtat aaaaagcaaa aagtatcgaa tttggtactt ggaaatatca
    15001 gcaatttgag taaagtaaac ttaaaagcaa ctgcataaga gcccctaatt atttacaata
    15061 tataaggggc taaaaggatt acagttgcag cgtttaaaga ataaactgta ccacaattgg
    15121 tgctgagaaa tataagtatt ttaaagcaaa gatttataaa agcaactgca taagagcccc
    15181 taattattta caatatataa ggggctctaa tatgctataa ttattgggaa aatgaaaatt
    15241 atatgtaaaa gaggtgagat atatgtttgt cgatcaagtc aaaatatctc ttaaagccgg
    15301 tgatggtggt aatggtatta ccgcatacag aagagaaaaa tatgtaccat ttggtggacc
    15361 agctggcggt gacggtggta aaggtgcttc agtcgtattt gaagtggatg aaggtttaag
    15421 aacgttatta gattttagat atcaacgtca ttttaaagca agcaaaggtg aaaatggcca
    15481 aagtagtaat atgcatggta aaaatgcgga agatttagta ttaaaagttc cacctggtac
    15541 aattattaaa aatgttgata cagacgaagt gttagcagat cttgttgaag atggtcaaag
    15601 agctgtagta gcgaagggcg gtcgaggtgg acgaggtaat tcacgttttg caacacctag
    15661 aaaccctgca cctgacttca gtgaaaaagg tgaaccaggt gaggaattag atgtatcttt
    15721 agaattgaaa ttattagctg atgtaggatt agtaggtttc cctagtgtgg gtaaatcgac
    15781 tttattatct atcgtttcaa aagctaagcc taaaattggg gcatatcatt ttacaacgat
    15841 taaaccaaac ctaggtgttg tttcaacacc tgatcaacga agttttgtta tggcagattt
    15901 accaggttta attgaaggtg catctgatgg cgttggatta ggacatcaat ttttaagaca
    15961 tgtagagaga acaaaagtta ttgttcacat gattgatatg agtggttctg aaggtagaga
    16021 acctattgaa gattataaag tcattaatca agaattagcc gcgtacgagc aacgtttaga
    16081 agatagacct caaatcgtag tagctaacaa gatggattta cctgaatcac aagataattt
    16141 aatcttgttt aaagaagaaa ttggcgaaga tgtgccagtt attccagttt caacaataac
    16201 gcgtgataat attgatcaat tattatatgc aatagcagat aaattagaag aatataaaga
    16261 tgttgacttc acagttgaag aagaggagtc ggttggcatt aaccgagtat tatataaaca
    16321 tacaccgtca caagataaat ttacaatttc aagagatgat gatggtgctt atgtggtaag
    16381 tggtaatgct attgaaagaa tgtttaaaat gactgacttt aacagtgatc cagcagtacg
    16441 tcgatttgct cgtcaaatgc gttcgatggg tattgatgat gcgcttagag aacgtggttg
    16501 taaaaatggt gatatcgtta gaattcttgg cggagaattt gaattcgttg aataggagcg
    16561 taacatgatg gacaataaag attataaaaa gttttattta attagagaag atgtcttgcc
    16621 tgaatccgtg gttaaaacat tgaagattaa agatgccttg aaaagtgatc cgacattgtc
    16681 catttatgat gccgttaaac agtttgatct atctagaagt gctttttata aatatagaga
    16741 aacgatattt ccagtagacg ataaaatgct tgaccatcga gaatttacat taattttata
    16801 tgtaactgat attgtgggta tgttggcacg tgtactagat gttatatcaa agttagaact
    16861 atctgtatta acgattcatc aaagtattcc aatggaagaa aaagcaacaa taacattatc
    16921 actgaatgct aaatctaaag aaacttcagt ggaggatgtt attggcgctt tgagaaattt
    16981 agattatgta tcaaaagtag aattaattag tatgagtatg taaggatgtg cctataatgt
    17041 acgcgtatgt caaaggtaag ttaacacatt tatatcctac acacgtagtt gttgaaactg
    17101 ctggtgttgg ttatgaaatt caaacaccaa attcttatcg ttttcaaaag catctagatc
    17161 atgaagtttt aattcataca tctttaattg ttcgtgaaga tgcacaatta ttgtatggat
    17221 ttagtagtga agaagagaaa gatatgttct tgagtttaat taaagttact ggtattggtc
    17281 cgaaatcagc tttagctatt ttagcgacaa gtacgcctaa tgaagtaaag cgtgccattg
    17341 aaaatgaaaa tgatacgtat ttaactaaat tcccaggaat tggtaagaaa acggcaagac
    17401 agattgtttt agatttaaaa ggtaaagtga aaatcactga agaagatagc gattcattat
    17461 tacaagtaga cgctacatcg acggtgcaag atcaattcgt gcaagaagca atgttagcgt
    17521 tagaagcatt aggttattct aaacgagagc ttgcaaaagt tgagaaaacg ttaaataaaa
    17581 ataaatatga ctcagttgat gaagctgtta aggcaggtct tcaattagtt gtatcttaat
    17641 tttaaataga ttaatagggg aagtgttgtc atgaatgagc gtatggttga tcaatcaatg
    17701 catagtgaag aaactgattt cgaattgtcg cttagaccta cgagattacg acaatatatt
    17761 ggtcaaaatt caataaaaag taatttagaa gtatttatta aagcggctaa acttcgtcat
    17821 gaaccattag atcatgtatt gctttttggc ccccctggat taggtaagac aacattatct
    17881 aatattattg ccaatgaaat ggaagtaaat atacgtacag tatcagggcc ttcattagaa
    17941 agacctggtg atttggctgc aattttatca ggacttcaac ctggagatgt tttgtttatt
    18001 gatgaaatac acagactgag tagtgttgtt gaagaagtgt tataccctgc aatggaagat
    18061 ttctttttag atattatcat tggtaaaggc gatgaggcta gaagtatccg tatcgactta
    18121 cctccattca ctttggtagg tgcaacaact cgagctggta gcttaacagg tccactaagg
    18181 gatcgatttg gtgtgcactt aagattagaa tattataatg aatcagattt aaaagaaatc
    18241 attattagaa cagctgaggt tttaggcaca ggtattgatg aagaaagtgc cattgaacta
    18301 gctaaacgtt ctagagggac tccaagagta gccaatcgac tattgaaacg ggtaagagac
    18361 ttccagcaag tgaatgaaga tgaacaaata tacattgaaa caacaaagca cgcattaggt
    18421 ttacttcaag ttgatcaaca cggactagat tacattgatc ataaaatgat gaactgtatt
    18481 attaagcagt ataatggtgg acctgttggt ttagatgcga ttgccgtaac aattggcgaa
    18541 gaacgtatta caattgagga cgtttatgag ccatttctta ttcagaaagg ctttttagaa
    18601 cgtacgccac gtggcagaaa agcaacacca ttagcttatg aacattttgc aaagtcgaat
    18661 gaggagagag aataacgtga atattgaaga atttgactat gacttaccag aatcattaat
    18721 tgctcaaacg cctttaaaag atcgtgatca tagtcgttta ttagtcatgg atagagaaac
    18781 tggtgaaatg aaacatttac atttcaaaga tatcattgaa tattttagac ctggtgatac
    18841 attagtgctt aacgatacgc gagtaatgcc agctagactt tttggtttaa aagaagaaac
    18901 tggtgcaaaa gttgaaatgt taatgc
//
LOCUS       DAPIDM010000037        18786 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000037 DAPIDM010000000
VERSION     DAPIDM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.037"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            355..492
                     /gene="rpmH"
                     /locus_tag="RJE42_001177"
     CDS             355..492
                     /gene="rpmH"
                     /locus_tag="RJE42_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV5876607.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            613..966
                     /gene="rnpA"
                     /locus_tag="RJE42_001178"
     CDS             613..966
                     /gene="rnpA"
                     /locus_tag="RJE42_001178"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV5876608.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            1110..2489
                     /gene="mnmE"
                     /locus_tag="RJE42_001179"
     CDS             1110..2489
                     /gene="mnmE"
                     /locus_tag="RJE42_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV5876609.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            2556..4433
                     /gene="mnmG"
                     /locus_tag="RJE42_001180"
     CDS             2556..4433
                     /gene="mnmG"
                     /locus_tag="RJE42_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV5876610.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFEIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            4433..5152
                     /gene="rsmG"
                     /locus_tag="RJE42_001181"
     CDS             4433..5152
                     /gene="rsmG"
                     /locus_tag="RJE42_001181"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV5876611.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKHQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            5195..6034
                     /gene="noc"
                     /locus_tag="RJE42_001182"
     CDS             5195..6034
                     /gene="noc"
                     /locus_tag="RJE42_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDV5876612.1"
                     /translation="MKKPFSKLFGLKNKDDILGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            6351..6704
                     /locus_tag="RJE42_001183"
     CDS             6351..6704
                     /locus_tag="RJE42_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDV5876613.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            6772..7167
                     /locus_tag="RJE42_001184"
     CDS             6772..7167
                     /locus_tag="RJE42_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDV5876614.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            7427..7996
                     /locus_tag="RJE42_001185"
     CDS             7427..7996
                     /locus_tag="RJE42_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5876615.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIIMGIGFYASMKIEDLKEVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVVGTLSLIIFLSVYLANKFL"
     gene            complement(8114..8314)
                     /locus_tag="RJE42_001186"
     CDS             complement(8114..8314)
                     /locus_tag="RJE42_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV5876616.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(8706..8897)
                     /gene="vraH"
                     /locus_tag="RJE42_001187"
     CDS             complement(8706..8897)
                     /gene="vraH"
                     /locus_tag="RJE42_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDV5876617.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSAVTTPMIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(8989..10869)
                     /gene="vraE"
                     /locus_tag="RJE42_001188"
     CDS             complement(8989..10869)
                     /gene="vraE"
                     /locus_tag="RJE42_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDV5876618.1"
                     /translation="MTFNHIVLKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     VIAYILILFQSSLFLKRRSILSMMKDSIKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNDFNVVTAQ
                     DAQQFESKLSQQQITFSKNAYETINVDNVKDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNVINIHLNKDLVVKGSKNETFRVTQEDKDKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKKKAQAIAKQFGDKIITYDDMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIIVIIVYTLIYIVFALIAFVHSNKLIKK
                     TI"
     gene            complement(10859..11617)
                     /gene="vraD"
                     /locus_tag="RJE42_001189"
     CDS             complement(10859..11617)
                     /gene="vraD"
                     /locus_tag="RJE42_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDV5876619.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            11907..12419
                     /locus_tag="RJE42_001190"
     CDS             11907..12419
                     /locus_tag="RJE42_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDV5876620.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(12722..13522)
                     /locus_tag="RJE42_001191"
     CDS             complement(12722..13522)
                     /locus_tag="RJE42_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDV5876621.1"
                     /translation="MNIAKLEDYLQIEPTRYDQPSIEALNYYATRFMLTVPFENIDVQ
                     NGKPISVDIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     QNGSHASLVVSINNVFYVTDVGFGDLPLNAIPITLPDDTQPITDISGTFRAIFNSEDK
                     DIFYVQKYENNHWHTKYEAEFKARQIEEFDQNIEYNQTHPDSVFVKHLLITMPQSFGR
                     ATMSENHLTLTKDGTPEKLAITKDNYKQFLTTYFGLNVTISRLENSDN"
     gene            complement(13762..14778)
                     /locus_tag="RJE42_001192"
     CDS             complement(13762..14778)
                     /locus_tag="RJE42_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDV5876622.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVIAALV
                     IGMIELLQILADKLDLHGAFWAFISSIEFNYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            complement(14937..15512)
                     /locus_tag="RJE42_001193"
     CDS             complement(14937..15512)
                     /locus_tag="RJE42_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV5876623.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEDVSQYTKSSLMEMHGVGPKAI
                     SILENALFQHQLHFKTEVHSSLPFLLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYKEIASLNIQSIITHGSLGSMHGSQILKT
                     GTVIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            15734..16642
                     /gene="rarD"
                     /locus_tag="RJE42_001194"
     CDS             15734..16642
                     /gene="rarD"
                     /locus_tag="RJE42_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV5876624.1"
                     /translation="MNTEYKKGIFLSLSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SVIFMIFILAVGQKQRNAFQRDMNQLLAKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKIVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(17192..18610)
                     /locus_tag="RJE42_001195"
     CDS             complement(17192..18610)
                     /locus_tag="RJE42_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV5876625.1"
                     /translation="MKNTVKYRKFILPIVVGLLIWALTPIKPEAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDESARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MAAQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
BASE COUNT         6475 a         2786 c         3453 g         6072 t
ORIGIN      
        1 cagcttggga aataatttat aaagtatgta taagaattgt ataaggtatt caaacattgt
       61 aaacactcat gcttcggacc aaaatcatgg tgatgttatg aagattgatt gctcgcatcg
      121 tgtatttcta tctttaatcg ataaaagtaa aaaaattgag cagtagtata aaatgaactt
      181 gcgttataaa aaggattttg ttatattgta gtagttgctt gaattatgac tagataatca
      241 atgagctaat acgagaattt taatctaaga aacgcaaaat cttatctatt aaagtgatca
      301 gagtctgcat aatctggtat attagataac tgtataaaga tggaggtgtt ttgcatggta
      361 aaacgtactt atcaaccaaa taaacgtaaa catagtaaag ttcatggttt cagaaaacgc
      421 atgagcacaa aaaatggccg taaagtttta gcgcgccgtc gtcgtaaagg ccgtaaagtt
      481 ttatctgcat aagatcactg actcatcagt gatctttttt ttcgtttaaa ttaagaataa
      541 atagaaattt atgttataag ctcaatagaa gtttaaatat agcttcaaat aaaaacgata
      601 aataagcgag tgatgttatt ggaaaaagct taccgaatta aaaagaatgc agattttcag
      661 agaatatata aaaaaggtca ttctgtagcc aacagacaat ttgttgtata cacttgtaat
      721 aataaagaaa tagaccattt tcgcttaggt attagtgttt ctaaaaaact aggtaatgca
      781 gtgttaagaa acaagattaa aagagcaata cgtgaaaatt tcaaagtaca taagtcgcat
      841 atattggcca aagatattat tgtaatagca agacagccag ctaaagatat gacgacttta
      901 caaatacaga atagtcttga gcacgtactt aaaattgcca aagtttttaa taaaaagatt
      961 aagtaagaat agggtagggg aaagggagga aaacattaac cactcaatac accccgaagt
     1021 cttacctcag acaagcgtaa gactaacccc agggatataa taacttattt taaaatgtat
     1081 tcacatcgtt gaaggaggcg agtagtaata tggatttaga tacaattacg agtatttcaa
     1141 caccaatggg tgaaggggca attggaattg ttcgattgtc tggaccgcaa gccgttgaaa
     1201 ttgctgacaa attatataaa ggaaaacatc ttttaaatga tgttccatca catacgatta
     1261 attacggtca tattattgat ccagagtcta aagaagtggt tgaagaagtt atggtgtctg
     1321 tgttaagagc accaaaaaca tttacacgcg aagatattat agaaattaat tgtcatggtg
     1381 gtattttaac gattaataga gtgctggaac taactatgac atatggcgct agaatggctg
     1441 agccaggtga atttacaaaa cgtgcctttt taaatggtcg tattgattta tctcaagctg
     1501 aagcagttat ggactttatt cgctcgaaaa cagatagagc ttctaaagtt gcgatgaatc
     1561 aaattgaagg tcgtctaagt gacttaatca aaaaacaacg tcaatctata ttagagatac
     1621 tcgctcaagt ggaagtgaat attgattatc ctgaatacga tgatgttgaa gatgcgacta
     1681 ctgaatttct tttagagcag tctaaagaaa tcaaacagga aattaatcgt ttattagata
     1741 ccggtgcgca gggtaaaatt atgcgtgaag gtttatctac agttattgtt ggtaaaccaa
     1801 acgtaggtaa atcatcgatg ttaaataatt taatacaaga taataaagcg attgtaactg
     1861 aggtagcagg tacgactaga gatgtcttag aagagtacgt caatgttcgt ggcgtgccat
     1921 taagattagt tgatactgct ggtatacgtg agacagaaga tatagtagag aagattggtg
     1981 ttgaacgctc tagaaaggct cttagtcaag cggacttaat tttatttgta ttaaacaata
     2041 acgaagcatt gactcaagaa gattacacat tatatgaagt ggttaaaaat gaagatgtaa
     2101 tcgtaattgt taataaaatg gatttagagc aaaatataga tattaatgaa gttaaagata
     2161 tgataggtga tacgccatta attcaaactt caatgttaaa acaagaaggt attgatgaat
     2221 tagaaataca aattcgagat ttgttctttg gtggagaagt acaaaatcaa gatatgactt
     2281 atgtttctaa ttcaagacat atttcattat taaaacaagc aagacaaacg atacaagatg
     2341 cgattgatgc agcagaatct ggtgtgccta tggatatggt acaaatcgat ttaactagaa
     2401 cttgggaaat attaggagaa attattggtg agactgcaag tgatgaactc atcgatcagt
     2461 tattcagtca attctgctta ggtaaatagt aattgtaata gacggaatac cgtcttaaga
     2521 aggctagtaa gatatcaaat aaggaggttt atattgtggt tcaagaatat gatgtaatcg
     2581 ttataggtgc gggacatgca ggtgtagaag caggtttagc atctgcaaga cgtggtgcta
     2641 aaacattaat gctaacaata aatttagata atattgcatt tatgccatgt aacccatctg
     2701 taggtggacc agctaaaggt atcgttgttc gtgaaattga tgctttaggt ggacaaatgg
     2761 caaaaacaat cgataaaaca cacattcaaa tgagaatgtt aaatactggt aaaggacctg
     2821 ctgtaagagc actaagagcg caagcagata aagtacttta tcaacaagaa atgaaacgcg
     2881 tgattgaaga tgaagaaaat ttgcatataa tgcaaggtat ggtagacgaa cttattatag
     2941 aagataatga agttaaaggt gtacgtacaa atattggtac agagtattta tctaaagcag
     3001 taattattac aacgggaaca tttttacgtg gtgaaatcat tttaggtaat atgaagtatt
     3061 caagtggacc aaatcaccaa ttaccatcaa tcacattatc agacaattta agagaacttg
     3121 gttttgaaat tgttcgtttt aaaacaggta caccaccgcg tgtaaattca aaaacaattg
     3181 actattcgaa gactgaaata caaccaggtg acgatgtagg tcgtgcattc agctttgaaa
     3241 caacagaata tatattagat caattgccat gttggctaac gtatactaat gctgaaacac
     3301 acaaagttat cgatgataat ttacatctat ctgcaatgta ttcagggatg attaaaggaa
     3361 ccgggccacg ttattgccct tcaattgaag ataaatttgt tcgatttaat gataagccgc
     3421 gacatcaact tttcttagag cctgaaggtc gtaatacaaa tgaagtatat gtgcaaggat
     3481 tgtctacaag tcttcctgaa catgtgcaac gtcaaatgtt agagacgata ccaggtcttg
     3541 aaaaagcaga tatgatgcgt gccggctatg caattgaata tgatgcaatt gtgccaacgc
     3601 agttatggcc tacacttgaa acgaaaatga ttaaaaactt atacactgca ggtcaaatta
     3661 atggtacatc tggttatgaa gaagcagcag gacaaggatt gatggcaggt attaacgctg
     3721 caggtaaagt gttaaacaca ggcgagaaga tattaagtcg ttcagatgca tatattggtg
     3781 tattaatcga tgatcttgta actaaaggta ctaatgaacc ttatcgttta ctaacatcac
     3841 gtgcagaata tcgtttgtta ctacgtcatg ataatgctga tttgagattg acggatatgg
     3901 gatatgaact tggtatgatt tctgaagaac gatatgcacg ttttaatgaa aaacgtcagc
     3961 aaattgatgc ggaaattaag cgtttatcag atattcgtat taaaccaaac gaacatacgc
     4021 aagcgattat tgaacaacat ggtggttctc gcttaaaaga tggtatttta gctatcgatt
     4081 tattacgcag acctgaaatg acttacgata taattttaga aattttagaa gaagaacatc
     4141 aattgaatgc ggatgttgaa gaacaagtag aaatacaaac aaaatatgaa ggttatatca
     4201 ataaatcact acaacaagtt gagaaagtta aacgtatgga agagaagaaa attccagaag
     4261 acttagatta tagtaagatt gatagtttgg cgactgaagc gcgagaaaaa ttatcagaag
     4321 taaaaccttt aaatattgca caagcttcta gaatatcagg ggtaaatcca gcagacatat
     4381 ctatattatt gatttactta gaacaaggta aactccaaag ggtgagtgac taatgactgt
     4441 agaatggtta gcagaacaat taaaagaaca taatattgaa ttaactgaga ctcaaaaaca
     4501 tcagtttcaa acatattatc gtttacttgt tgaatggaat gaaaagatga atttgacaag
     4561 tattacagat gaacacgatg tatatttgaa acatttttat gattccattg cacctagttt
     4621 ttattttgat tttaatcagc ctataagtat atgtgatgta ggcgctggag ctggttttcc
     4681 aagtattccg ttaaaaataa tgtttccgca gttaaaagtg acgattgttg attcattaaa
     4741 taagcgtatt caatttttaa accatttagc gtcagaatta caattacagg atgtcagctt
     4801 tatacacgat agagcagaaa catttggtaa gggtgtctac agggagtctt atgatgttgt
     4861 tactgcaaga gcagtagcta gattatccgt gttgagtgaa ttgtgtttac cgctagttaa
     4921 aaaaggtgga cagtttgttg cattaaaatc ttcaaaaggt gaagaagaat tagaagaagc
     4981 aaaatttgca attagtgtgt taggtggtaa cgttacagaa acacatacct ttgaattgcc
     5041 agaagatgct ggagagcgcc agatgttcat tattgataaa aaaagacaga cgccgaaaaa
     5101 gtatccaaga aaaccaggga cgcctaataa gactccttta cttgaaaaat aatgcataat
     5161 cctttacaac taacataaaa ggagcgaatg gataatgaaa aaaccttttt caaaattatt
     5221 tggtttgaaa aacaaagatg acatccttgg acatattgaa gaagatcgca atagtaatgt
     5281 tgaatccatt caaattgaac gtatcgttcc caaccgttat caaccaagac aggtgtttga
     5341 accaaataaa attaaagaac ttgctgaatc aatacatgaa catggcttat tacagcctat
     5401 tgttgtaaga ccgattgaag aagatatgtt tgaaattatt gctggagagc gccgatttag
     5461 agcaatacaa tcactaaatt tacctcaagc agacgttatt attcgtgata tggatgatga
     5521 agagacggct gttgtggcat taattgagaa tattcaaaga gaaaatttgt ctgttgttga
     5581 agaagcggaa gcctataaga aattattgga aattggtgat acaacgcaaa gtgaattggc
     5641 aaaaagttta ggtaaaagtc aaagctttat tgcaaataag ttgcgtttat tgaagttggc
     5701 gccgaaagta ctacttcgct taagagaagg taaaattact gagcgccatg cgagagcggt
     5761 attatcattg tctgatagcg aacaagaagc gttgattgag caagtcattg cacaaaaact
     5821 aaatgtgaaa cagactgaag atagagtacg ccaaaaaacg gggcccgaaa aagtcaaagc
     5881 acaaaacctt cgctttgcac aagatgtcac tcaagcacga gatgaggtag gcaaaagtat
     5941 ccaagcgatt caacaaacag gattacatgt tgagcataaa gacaaagatc atgaagatta
     6001 ttatgaaata aaaattcgaa tatataaacg ttagtagtag gatgtcgtat acatgatgac
     6061 taacacataa aagacaaagc taagatcata acagctttgt ctttttttgt tttacgtgaa
     6121 acataaaaat ttatatttat atgttgattt gaaaattaca gttaatggta attttcaata
     6181 atttgagatg gatatacctt tatttgaacc ctttattgct agtttatgaa agtatcatga
     6241 aagctttatg aacatcgctt gagttgcctt tacagtagaa aatttaagtt ttacactttg
     6301 tgtgaatgat acgttttgta ttgaattaat tatagaaagg tacgttgaag atgttttcaa
     6361 ttggaagtgc aattcttcat tttgtcattg gtggtatcgc tgttgcatta gcttcaatta
     6421 ttgctgataa ggtaggtggt aagttaggag gtattatagc tactatgccg gcagtctttc
     6481 ttgcggctat tatcgcatta gctttggatc atcgtggtac gcaattagtg gagatgtcga
     6541 tgaatcttag tactggagca attgtcggta ttctgtcttg tatattaact gtatttttga
     6601 catctctcta cattaagcat aaaggttatc ggaaaggcgc aatattcaca gttgtttgtt
     6661 ggtttgttat ttccctcgca atattcagta ttagacattt atagtttgga aaatacgtga
     6721 taattagttg tattcagtta ttaagtaata aattattgga ggcagaacat catgaaatta
     6781 acattaatga aattttttgt ggggggattt gcagtattat taagttatat tgtatctgta
     6841 acactacctt ggaaagaatt tggcggtata tttgcaacgt ttccggcagt atttttagtg
     6901 tctatgttta ttacaggtat gcaatatggt gataaagtcg ctgtgcatgt aagtcgtggc
     6961 gcagtatttg gtatgacagg ggtattagtt tgtattttag ttacatggat gatgttacat
     7021 atgacgcaca tgtggttgat tagcattatt gttggtttcc taagctggtt catcagtgcc
     7081 gtatgtattt ttgaagcggt agaatttata gcacaaaaaa gattagaaaa gcatagttgg
     7141 aaagctggaa aatcgaatag taaatagtgt gaacgtaatc tcttaaatag gactaacttc
     7201 gcaagcattg aatagcatgg aaaagtggca tcattaataa gtgaaattca agttggcatt
     7261 gagaaaatta caagcgcgta atcatacaag tttgtcttaa gggagtcttc gaacccttat
     7321 gttgtcgtat gtcaaaacat ttagtcaatc atacaggtga cttgatttaa ctttatctga
     7381 tagtctgatt gtaatgattg tactaattga atggaggcgt atgtaattga atctgagtaa
     7441 acaaattaaa aagtataggg aacgagatgg ttattcacaa gaatatcttg ctgaaaagtt
     7501 atatgtatct aggcagagta tttctaattg ggaaaatgac aaaagcttac cagacataca
     7561 taacttatta atgatgtgcg aattgttcaa tgtaacttta gatgatttag taaaagggac
     7621 tattccattt gtacctgata ttaaagcgca acgaagtctt aacttatgga catatgtgat
     7681 gcttattttc atgacattag ctgcaatttt aatgggacct ttagttgttt attggaattg
     7741 gacttggggt ataacggtgg caatcattat gggaataggt ttttatgcat ctatgaaaat
     7801 agaagattta aaagaagtgc ataaaatgga caactacgat cgaattgttg cttttatgaa
     7861 tggaaaagat cctagtgaag tacaaacgac taaagctaga aatacgatga caaatgcgct
     7921 ttctattata tcagtagttg gtacactcag cctcataatt ttccttagtg tgtatctggc
     7981 aaataagttt ttataaatca tcatcgtatc gtctcatatt atccaaaata gcataaaaaa
     8041 ataccaacaa gatttagaac cttgttggta atcaaagcga ttcatttata atgagtcgtt
     8101 ttattttgta agattaaaca gtttgtacgt taactgcttg gtctccacgt tgaccttcag
     8161 tgatttcgaa agtaactttt tgaccttctt ctaaagtttt gtagccatcg ctagcgatac
     8221 ctgagaaatg tacgaatacg tctccgccat tttcttgttc gatgaaacca aaaccttttt
     8281 ctgcgttaaa ccattttact gtaccgttat tcatatagaa aacctccgtg tgcttttgca
     8341 cttaatattt gtaacaaatt cataactaaa aaagaggata ttctaaacaa atacactaca
     8401 atttaattca cgagctttta ttacgtaaga ccaactatac gctcatattg gcataatgta
     8461 cagtgttttt tgaaaataaa ttaaaaaaga tttttaaaaa ccttagaaac gttgatttaa
     8521 aggggtttat aaaaataaaa aattgtagtc ttttatggtg tttgctagtt ttcaaagtga
     8581 cataacgttt aaacatgacg attttataag caattcataa aaaacaagca gcgataaacg
     8641 ctacttgttg atattaaaat ctgacatgaa aggtcatagc aatgtaatat actgatggaa
     8701 tacgtttatt tacctttttc ttcgcggcgt tttaaataat aagcgccacc taataaacca
     8761 gctggaatac ctatcattgg tgttgtaacg gcgcttaaga caataacaat cattgctaaa
     8821 gcaataacgt tataccaagt tacagtcaaa tttttcaaat cctcgtatga ttgttttact
     8881 aattctctaa atttcatgat tcaatctctc ctttttataa attagttaat agttatactg
     8941 cattgcttaa aatcaaaatc tcaatgaagc atcttttaat cacaagtgtt aaatggtttt
     9001 cttaatcaat ttatttgaat gtacaaaagc aattaaagca aaaacaatgt aaatgagtgt
     9061 gtatacaatg attactataa taaccggcat gaaggatata ttacccatca atttcataaa
     9121 tgctattgcg gcgaatactg catgtaatat agcaatcaat aatggtaaac cgaagttgaa
     9181 agtaattttt aataataatc ccttaagcat atctgtatgc gtaaagccta tgcgttttaa
     9241 tattctgaag ttacttagtt catcctcagt ttcatccatt tgtttaatat aaataataca
     9301 tccagctgct actagaaatg ctaatcctaa aaatgatgta acgaatatta gaataccgtt
     9361 agtagcatcg acctcttttt tcatgtcatc ataagtgatg attttgtctc caaactgttt
     9421 tgcaattgct tgagcttttt tcttttgtga tgtttgttta atatcatatc cataaaaagt
     9481 atgaacatta ttttgtgttt tcaattgctg atacttttct ggacttactt caatgacagg
     9541 tgaattaaag cttagattta aaggataaac cttgtctttg tcttcttgtg taacacggaa
     9601 agtctcgttc tttgaacctt ttactactaa atctttgttt aaatgaatat taatcacgtt
     9661 aggcagcgat tttgtatttg taatgatggc attgttgcct gttaacttgt tatttgcact
     9721 taatattgaa ttcgtgcgac ctgaatcact accattttcc aaagtaataa cctgatcttt
     9781 aacattatct acattaatag tttcgtaagc atttttagaa aatgtaattt gttgttggct
     9841 tagtttagat tcaaattgtt gcgcgtcttg agcggtgacg acgttaaaat cattgggtgc
     9901 catagatgta agggtttgat cggtatttga tttagataac gctgcgaaac acaatacagt
     9961 tactgtaact gcagaaataa ttgcaatgat agttaaagac atggcatttt tcttcattcg
    10021 gtacataata gacgatgtaa atacaacatc tgtaatagat acgcgtccat ttttagattt
    10081 tttcaaagtt ttaaaaatca gtgacacgga acttctgaag aataaatagg cgcctacaac
    10141 cgttaaaaat aaaatgataa acggtgatgt catagccata gttagtgctt tgaacgtacc
    10201 aaacatttct gtcgccatat aatatcctag tgcaatcata gcaataccta atacgcctga
    10261 aatgacttct ataactgtaa ctttcgcagt tgtaacatca gtcttaattg agtctttcat
    10321 cattgataag atactgcgtc tttttaaaaa taaactactt tgaaatagaa tcagtatata
    10381 cgcaataact agcatgaaaa tagttaaaac aagggccata ggttcgaaat gtatcgataa
    10441 gttaatcgat aacgacatca atttagatac aatggaaagc aataattgtg cacctgcaat
    10501 gccacataat acaccgacaa cacctgtgat taaaaataca atcatttgtt caagtgctaa
    10561 cattttcaaa atgttttgtc gtgttaaacc aatcaactga aatagcgcaa attcacgtgt
    10621 acgacgtttc acgaataaat gattggcata cattaaaaag atgacaataa tgataaataa
    10681 aaatattgat ccgactaaag cacctttctt aatgatagcc atcgagtcgt cattatttac
    10741 acctttagta aactgtaagg ttgtaaaact gaaatataag acgatgctaa aaaataatga
    10801 aaataaatac attgcataat gttttaagtt ttgacgtaag ttttttaaaa cgatatggtt
    10861 aaatgtcatt tgagacacca cctaatactg attgaagatg tacaatgtct tcataaaagg
    10921 cctgtttaga acgtccttcc tgataaagtt gtgtatgaat ttggccatct tttagcatga
    10981 tgacacgttc tgcaaaactt gctgcaaccg gatcatgtgt aaccatgaca attgtggtat
    11041 caaacgattt attcatttct tccaaacgtt gtaataaatc atttgcactt ttcgagtcga
    11101 gtgcgcctgt tggctcatct gcaaatatga tttgtggttt gtgaacaaat gctctcgctg
    11161 ctgcagttct ttgttgttga ccaccagata attcgctagg gtatttattt cccaggtcat
    11221 aaatacctaa tgctgtcgta atcgctttat aattttcttc cattgttgct ttcgacattt
    11281 tttgaacaga taaaggtaac ataatgtttt ctttaacagt taatgtcggc agaatactgt
    11341 aatcttggaa gatgaaacct aatgattctt tacggaattt ggcaagtgct ttttgattaa
    11401 gtttattaag ctcttgtccg ttagcaatca cgctaccgct agaaatttgg tcaattgaac
    11461 ttagtacatt taataaggtt gtcttacctg atccagaagg ccccataatc gcaacgaatt
    11521 cgcctttttg tatgtcaaag ttaatatctt taagtgcttg aaatgtgtgc tttttaccgt
    11581 atgtttttga aacatgttgc actgataata tcgtcatagt atcactcctt ttgtatttaa
    11641 tttcatttta aataataagt agaacagtag cctttatcta aacttacaat tcaatgtttg
    11701 aatcttacat agttgaaaga tattaatata tcgtgatttt ctcgggtgaa tttcgattat
    11761 tggaaagcag ttcaactaat ttgagcatca cagtatttgc actgaggaaa tgtttctata
    11821 agttaagatg agggaattta aacaatgaat aatagtgcca ctaatgaaaa tataaagagg
    11881 gcgttgtttg aaaggaaatg attgttatgg aaaaaggaaa tcaaggcatt aaatggtcta
    11941 gtttgattat gggtgtgttg ttattaatgt tagcagttgt tatttttaca ttcccaattg
    12001 aaaactttta tgcaatcaca tggttaattg gattatttgt attgattaat ggtgtgattc
    12061 aaattgttta tcgtagaaaa gccaaagctt tagttggtgg taaccaaaat tggattctgt
    12121 ttatggggat tgtagatatt ctatttggtc ttattgtcat ttttaatgtt ggtgctagct
    12181 cggcattttt tatctacatg tttgcatttt ggtttatttt tagttctatt gctggattat
    12241 ttacgttttc aggtagtggt agcttaaaac taatttcagt gatttttaat ttattaggta
    12301 ttgttttcgg tgtcatttta ttatttaatc cattaatggg tatcgtcttt atttcgacga
    12361 tgattgctat tgcatttgta ttcgtaggtg tcatttatgt tgtagatgca cttgcttaag
    12421 taaaatgaag cggttcaaaa gaagggtgtg gcatgaagtt tgtgccatat cctttttgtt
    12481 gtgtttgcac agagtgtcaa taaagaaata ttttatcttt agaaactagt attttttatg
    12541 catgcgtttt acgcatgaat gtctatttat aagtacacat gagctctggc taatgtgtga
    12601 gaaccactaa atcattaatt gttagtggcg ctgtatcatt tttgtcccac atcctaacga
    12661 acatgtgtat ttaaaaagga cgttacctac attaaagtaa gttacgtccg tatttttatg
    12721 attaattgtc gctgttttca agtcgactaa ttgttacatt tagtccaaag tatgttgtta
    12781 aaaattgttt atagttatct tttgtgatag ctaacttttc tggcgttcca tcttttgtta
    12841 aagttaaatg gttttcagac attgtagcac gtccaaatga ttgtggcatt gtaattaata
    12901 aatgttttac aaatactgaa tcaggatgtg tttgattata ttcaatgttt tgatcgaatt
    12961 cttctatttg tctagccttg aattcagctt cgtattttgt atgccaatga ttattttcat
    13021 atttttgaac atagaaaatg tccttatcct cactgttgaa aatagcgcgg aatgtaccac
    13081 tgatatcagt aatcggctgt gtatcatctg gtaaagtaat tggtatggca tttagaggta
    13141 agtctccaaa gccgacatcg gttacataaa agacattatt aatagaaaca acaagtgatg
    13201 catgtgaacc gttttgacta cgaccgccac cgggtgtatg aatggtagcc gacattaatt
    13261 ctgggttaaa tcctttttgt tgtaaatatg ctttgaaaaa tgtgtttaat tcataacaaa
    13321 agccaccacg tttgtcatga acaattttat tgaaaagtgc atcgatatct acagatattg
    13381 gtttaccatt ttgaacatca atattttcga aaggcaccgt taacataaag cgagtcgcat
    13441 aataatttaa tgcttcaata ctcggttgat cataacgagt tggttcaatt tgtagataat
    13501 cttctaactt tgcaatattc attagtatac cgcctcctgt attaaagact ataagagaat
    13561 tgtaaacaga aatgataaaa ttttaaataa gaaagcactg gattttacaa gatgtatttt
    13621 agatagaaaa atacgctctc aatgctgtca tctttaaaag taagattcaa aatatgacaa
    13681 tagagagcgt atcctaaagg aggcagtatg cagggtgtct aaaaacgcaa taataaaaag
    13741 atgattgcaa gacaagctac tttatttaga ccatttgtgt tctatgcgac caaacttcca
    13801 aattaagctt gaaataagcc aagttattaa aaataaagcg actagaatat agcctaaata
    13861 attaaattcg attgaactaa taaatgccca aaatgcaccg tgtaagtcta gcttatcagc
    13921 aagaatttgc agcaattcaa tcatcccaat cactaatgca gctatgacgg atatcgcagt
    13981 aatcgttata ttgtaataga ttttgcgaat aggattgaag aatgcccaat tataggcata
    14041 tttcattaca acaccatcta aagtatccaa caaactcata cctgatgcga ataaaattgg
    14101 taaagataag attccgataa atgaaatggc ttgttgtgac gcgcctgaag aaagagcaag
    14161 taacgcaatt tcactagctg tatcaaaacc aagtccaaat aaaaagccaa gtggcaatac
    14221 gtgccaacta cgcgtgatta atttgaaata aggtcctaca aatcgagaaa caaatcctcg
    14281 agattcaagt aatgcatcga cttcagcttc ttcaatgtgt tcacgacgta atttagcgaa
    14341 caagttaatt aaagagatta aaataattag attcaacaca ccgataagca ctaaaaagaa
    14401 acctgaaact agtgtaccaa tcgttccacc aatatcttgg aaatgtggta attcatcttt
    14461 agcccatttt acagataccc ctaaaaaaac agccattaaa aatacaacag atgaatgtcc
    14521 aattgaaaag tagaaaccaa caccagatgg atctttccgt tgctgtaata atttgcgaac
    14581 cgtattatct attgcggcga tatgatcagc atcaaatgca tgacgtaaac ctaatgtata
    14641 tgcaagaatc cccataccaa ataagatatg atggtctttt ccagcaatcc ataaaaaact
    14701 aaacccaata acgtgtaaca aaatgacaat agctatgtat ggtaaccagc ttaagcgctc
    14761 atttttaaaa acagtcaaaa tatatccttc tttcataaca tgatatttaa tcatactgta
    14821 tgttcaatgt gcactctagt aaaagtattc atataacaaa aatattatgc aaaattattt
    14881 gtattattaa tatatatgta attacgatag tttttagcgt tatagattat gagtcattaa
    14941 ccaacaacaa tgtaagatgt cactttgctt aacttggcat cctttttatg attttcaaat
    15001 tcaaaaaaat gagcaaaatg aatcactgta ccagttttta atatttgact accgtgcatg
    15061 gaacctaagc tcccatgtgt gatgatggat tgaatattga gagaagcaat ctctttataa
    15121 tgattagata gttcttgaat taatatttga ggtccgtata tgtcaaagcg gccagggaca
    15181 gaccaaataa attctgttgt aaccagtgaa cgtaataatt caatatctaa tgctgctgta
    15241 gcaactataa aatctatcat ttgttgacgt ttaggcgcat gattgcatga gacatctcct
    15301 gttaataaaa aaggtaatga tgaatgaact tccgttttaa aatgtagttg gtgctgaaat
    15361 aaagcatttt ccaatattga tatagctttg ggacctacgc catgcatctc cattagagat
    15421 gactttgtat attgtgatac atcttctaat gtgtatatac cttgtgaatt tagcgcacgt
    15481 gttgcaggct ttccaatttt aggtaaagtc atttgtatcc atcctttatg ttgtttggta
    15541 gtctttctgg caatataccc tgatttatac agaattattt ataaaattaa tattatttag
    15601 tggttagcga ataaatgtgg aaaatataaa caaaatgata tctttatcga aatattatta
    15661 gtaaaatcgt tatattagct taactaagtg agcgtaacat gttaaaaatt tgggagagta
    15721 aggaggtatg taagtgaata cagaatataa aaaaggtata tttttatcac ttagtgctta
    15781 cattctttgg ggcatactac ctatatattg gcagttcgtt gatgcaatag gcgcatttga
    15841 aattttagcc tttcgtatta tattttcagt aatattcatg attttcatac tcgcggttgg
    15901 acaaaaacaa cgcaatgcat ttcaacgaga tatgaatcaa ttgttagcca agcccattca
    15961 attactagca attgtcgtag caggctatgt cattacatta aattggggta catttatttg
    16021 ggctgtaacg aacggtcacg tactacaaac aagtttaggt tattatataa atccacttgt
    16081 tagcattttg ctcgcactta tctttttaaa agaaagattc aataaatttg aatggctagc
    16141 catattattc gcattcattg gtgtattata tatgacactg aaaattggag aattcccaat
    16201 cgtctctatt atattagcgt tatcctttgg tacatacgga ttattaaaaa aaatagtaca
    16261 tattgatgcc atcagcagta ttacgattga atgtattgtt accgcacctg ctggactaat
    16321 atacgttatt tatttatggc agcaacatca gatgtcattt ggattgaaca tgtcatcatt
    16381 ttggttgtta ttttctggtg ctattacggc aataccacta atcctattct cagccggggc
    16441 aaaacgtatt ccactttcgc taataggatt tattcaatac gttggaccaa caataatgtt
    16501 tgtactcggt atatttgttt tcaaagagcc ttttagtata gatcaattaa ttacgtttat
    16561 atttatttgg acaggtattg tgttatatag tctttctcaa tacattaaat tgaagaaaca
    16621 tccggtcgca aaaaccctat aaaatgcgag gagagataat tatacgtgtt ttcaacttcc
    16681 tttgtgtcat gttaaggtga gactgaggta tgtgtgcagt aatagtgaca gccgtataaa
    16741 tttgaaacat taaatataaa ataaagcact ttctttgaat tcataattaa atgaatttaa
    16801 acgaaggtgt tttttatttt gtgatttatg agggcataag taatgttaat caacgaagtg
    16861 cgtttataca gaaatgtatt ttctgtgatg ttacacaatg atttaaagcg ttatatagat
    16921 ttttagtgaa gcatgcaaat gatttgaagt atgaatgtat tttaaaaagg gagtcttgat
    16981 ttagcaaaga ttgattttaa atacaaatta aaaacaggct gggacataaa tcaatgttct
    17041 atgctctacg atgttatatt ggcagtagtt gactgaacga aaatgcgctt gtaacaagct
    17101 tttttcaatt ctagtcaacc ttgccggggt gggacgacga aataaatttt gcgaaaatat
    17161 catttctgtc ccactcccta atttaaacat tttaaaatat accaattact ttcatccaaa
    17221 gtgatcctaa accaatccag ataataaagt agacgaaacc taatattaag ttcattgtcc
    17281 accaacgttt ttgagtcacg taacctgaag agaatagaat cggcgctgga ccactactat
    17341 agtgtgttgt tgaagctaat aggttaccaa agaaacctaa cattaatgca ctgaataatg
    17401 gtggtgcacc ggctgcgatg gcaacgccta gtaatgctgc atacatcgca ctgatatgtg
    17461 ctgtagaact tgcaaataag taatgtgaat agaagtagaa caatattaaa atgaccagta
    17521 ctataggcca gcttaagcca ccaagacttg tagcaataga tttacttaac caaggaataa
    17581 aaccaagttt gtttaattgg gcggccatta acactaacac tgagaaccat actaatgtgt
    17641 tccaagcacc tgtttcgttt aaaatgtctt gccatgttaa gacgcctgtc aataataaca
    17701 atgctagcgc aataaaggcc gttaaagttg catcaatatg aatgaaactt ccgacaatcc
    17761 atagtgttag agcaatgaca aaaataccaa tcataaattt ttcagctaaa gcgattttac
    17821 ccatagtcgc taattcattt tcagcccaac ttttagcatt aggtgtttct ttaacagttg
    17881 gtggataaat tttataaatg ataaaaggta caacaattaa ggaaacaagt ccaggaacta
    17941 aagcagctaa gaaccaattc atccatgtaa tgtgaacatt agatgtgcta gatgctaaat
    18001 tttgtgcaag gggattaccg gccattgcag ttaaaaacat agcagcagta attaagttac
    18061 cttggaattc tgtgaaaaca agaaatgcac ccattttgcg tgctgattcg tctttcggtt
    18121 tcgaaccaaa tgattcagaa agtgatttga taattgggaa catgattcca cccgcacgcg
    18181 cggtattact tggtgtagca ggcgctagaa ttaaatctac accgacgata gaatatgcta
    18241 agcctaatgt ttttttacca aataatttga cgaaatgaag tgcgatacgt ctaccaagac
    18301 ctgttttcac aaatcctctc gaaatgaaaa atgccatagc aattaaccat atactattat
    18361 taccaaaacc agcgacagcc gttttcatgt caacaatgcc aacgagcacc atgattgtaa
    18421 atccaattat agagacagcc ccgattggca tcggttgtgt aatacaagca atgattgtcg
    18481 cgacgaatat tgcgaacata taccatgctg ttggatccac agcttccggt ttaataggtg
    18541 taagtgccca aataaggaga cctacaacga tagggagtat aaacttacga tatttaaccg
    18601 tgtttttcat gttaaaacgt ccttctttct atgttttata catatttcaa tttaagaata
    18661 aagctaacta caaaagatgt acagtaataa ttaaatataa aattaaatta acgaaatcat
    18721 taatataatt atttttcgag aagcggtgaa gaactggtat agttggtgtt tattaaattt
    18781 aaaaga
//
LOCUS       DAPIDM010000038        18287 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000038 DAPIDM010000000
VERSION     DAPIDM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.038"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..73)
                     /locus_tag="RJE42_001196"
     CDS             complement(<1..73)
                     /locus_tag="RJE42_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HDV5876626.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFY"
     gene            372..860
                     /locus_tag="RJE42_001197"
     CDS             372..860
                     /locus_tag="RJE42_001197"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV5876627.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            853..1788
                     /locus_tag="RJE42_001198"
     CDS             853..1788
                     /locus_tag="RJE42_001198"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV5876628.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIEEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDINLKIYDAELVETNKINEPGTIIDTTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            1785..3092
                     /gene="rsmB"
                     /locus_tag="RJE42_001199"
     CDS             1785..3092
                     /gene="rsmB"
                     /locus_tag="RJE42_001199"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV5876629.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEGTETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFKHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVDLQLEILENVKNNVKTGGEIVYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            3095..4189
                     /gene="rlmN"
                     /locus_tag="RJE42_001200"
     CDS             3095..4189
                     /gene="rlmN"
                     /locus_tag="RJE42_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDV5876630.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            4196..4939
                     /locus_tag="RJE42_001201"
     CDS             4196..4939
                     /locus_tag="RJE42_001201"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDV5876631.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            4936..6930
                     /gene="pknB"
                     /locus_tag="RJE42_001202"
     CDS             4936..6930
                     /gene="pknB"
                     /locus_tag="RJE42_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDV5876632.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPTPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKTVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDIAIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKAIEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDI"
     gene            7158..8033
                     /gene="rsgA"
                     /locus_tag="RJE42_001203"
     CDS             7158..8033
                     /gene="rsgA"
                     /locus_tag="RJE42_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDV5876633.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYKRKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQLEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            8034..8678
                     /locus_tag="RJE42_001204"
     CDS             8034..8678
                     /locus_tag="RJE42_001204"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV5876634.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            8685..9326
                     /locus_tag="RJE42_001205"
     CDS             8685..9326
                     /locus_tag="RJE42_001205"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDV5876635.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVIVHDGLILQIRSTDLN"
     gene            complement(9707..9895)
                     /locus_tag="RJE42_001206"
     CDS             complement(9707..9895)
                     /locus_tag="RJE42_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV5876636.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            10338..10712
                     /locus_tag="RJE42_001207"
     CDS             10338..10712
                     /locus_tag="RJE42_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDV5876637.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            10727..12373
                     /gene="fakA"
                     /locus_tag="RJE42_001208"
     CDS             10727..12373
                     /gene="fakA"
                     /locus_tag="RJE42_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDV5876638.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINLKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAVEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            12563..14623
                     /gene="recG"
                     /locus_tag="RJE42_001209"
     CDS             12563..14623
                     /gene="recG"
                     /locus_tag="RJE42_001209"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV5876639.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFSSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDGLREKYKLETLDFTLNTLHHPKSKEGLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLI
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQNYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            14826..15398
                     /gene="fapR"
                     /locus_tag="RJE42_001210"
     CDS             14826..15398
                     /gene="fapR"
                     /locus_tag="RJE42_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDV5876640.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            15403..16389
                     /gene="plsX"
                     /locus_tag="RJE42_001211"
     CDS             15403..16389
                     /gene="plsX"
                     /locus_tag="RJE42_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV5876641.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            16382..17308
                     /gene="fabD"
                     /locus_tag="RJE42_001212"
     CDS             16382..17308
                     /gene="fabD"
                     /locus_tag="RJE42_001212"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV5876642.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLYNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPSGVGSMAAVLGLDFDKVDEICKSLSYDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMTLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            17301..18035
                     /gene="fabG"
                     /locus_tag="RJE42_001213"
     CDS             17301..18035
                     /gene="fabG"
                     /locus_tag="RJE42_001213"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDV5876643.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
BASE COUNT         6719 a         2498 c         3562 g         5508 t
ORIGIN      
        1 cataaaattt atgaatacca aaactaccta agaacaatgc taataaaata taaataactt
       61 tgtttacttg catttcttac cctccagttg aattgcttat aatgacatta gcttctcttt
      121 ttattagagc cacttttagt tcaaacattc tagtttaagc attcccaatc atctaaattt
      181 cagttattca atccttacaa taaatttatg attacacttc agttgcattg tattatttta
      241 cgtgtgaaat atacgtaatg aatcacatga caatctttaa attgaaaaat atacattcta
      301 tgatgtaagg tcgcattttt aatatattta cgttataatg gtttgatgaa ttttattgag
      361 gagtaagagc tatggcgatt aaaaagttag tacctgcatc gcatcctatt ttaacgaaaa
      421 aagcgcaagc agttataaaa tttgatgatt cgttaaaaag attattacaa gatttagaag
      481 atacaatgta tgcacaagaa gctgctggct tatgtgcacc tcaaattaat cagtcattgc
      541 aagtggcaat cattgatatg gaaatggaag gattattaca acttgttaat ccgaaaatta
      601 ttagtcaatc aaatgaaacg ataacagact tagaaggttc aattacattg ccagatgttt
      661 acggcgaagt gacaagaagt aaaatgatcg ttgtcgaaag ttatgacgtc aatgggaaca
      721 aagttgaact aactgcacat gaagatgtag caagaatgat tttgcatatt atagatcaaa
      781 tgaacggtat cccttttaca gaacgtgcgg accgtatttt aacagataaa gaagtggagg
      841 catattttat aaatgactaa aataatattt atgggtacac cagacttttc aacaactgtt
      901 ttagaaatgc tcattgaaga acatgatgtc attgcagtcg taacgcaacc agatcgacct
      961 gttggacgta aacgtgttat gacaccacca ccagttaaaa aagttgcaat gaaatatgat
     1021 ttacctgtat atcagcctga aaaattaagt ggatcagaag aattagaaca attgcttcaa
     1081 ttagatgtag atttaattgt aactgctgct tttggacaat tattacctga atcattgttg
     1141 gcattaccaa aacttggcgc aattaatgta catgcatcat tgttaccgaa gtatagaggt
     1201 ggtgcaccaa ttcatcaggc aattatcgat ggtgaacaag aaaccggcat aacaattatg
     1261 tatatggtta aaaaattaga tgcgggtaat attatttcgc aacaagcaat taaaatagaa
     1321 gaaaatgata atgtcggtac gatgcatgat aaattaagcg tattaggagc agatttatta
     1381 aaagaaactt taccatctat tatagagggc acaaatgaaa gtgtacctca agatgatacg
     1441 caagcaacat ttgcttcgaa tattcgacgc gaagatgagc gaattagctg gaataaacca
     1501 ggaagacaag tgtttaatca aattcgagga ttatcaccat ggccagttgc ttatacaact
     1561 atggatgaca ttaacttgaa aatatacgat gctgagctcg ttgagactaa taagataaac
     1621 gaacctggaa caattataga cacgactaaa aaagccatta ttgttgctac aaatgataat
     1681 gaagctgttg caattaaaga tatgcaattg gctgggaaaa agagaatgtt agctgccaat
     1741 tatttaagtg gtgcgcaaaa cacactagta gggaagaaac ttatatgata gaaaacgtga
     1801 gaagtcttgc ttttgacacg attcaagata tattaaatga aggtgcgtat agtaacttgc
     1861 gtatcaatga agtgttgtca gaaaatgaat taaatgcaat ggataaagct ttatttacag
     1921 aaattgtcta cggaaccgtt aaaagaaaat atacgttaga tttttattta aagccttttg
     1981 tgaaaacgaa gattaaggca tgggttaggc aattattatg gatgagtatt tatcaatatg
     2041 tttatttaga taaagttcca aatcacgcca tcattaatga agcagttgaa atagcaaaag
     2101 aacgaggtgg ctatcacaac ggaaatgtcg taaatggcat tttacgaaca atgatgcgta
     2161 gtgacttacc tgattttaat gaaattgcag atcctaaaaa aagaatggca atcgagtata
     2221 gtatgccgaa gtggattata gatcattggg caacacatta tggtctcgaa ggaactgaaa
     2281 caattttaca gtcattttta gaaacgacat caacaactgt gcgtgccaac ctgacgcgag
     2341 catcattaga tgatattatt gaaaagttgc aagacgaagg ttatgacgtt gaaaaagatc
     2401 atgacttacc ttattgtctc catataggag gacaaccaat tattcattct cgttcattta
     2461 aggatggatt cgtttcaatt caagataaaa gctcaatgtt tgttgcacac attatgaatg
     2521 tagaccgaca tgatcacgta ttagatgcat gtagtgctcc tggcggtaag gcttgtcaca
     2581 ttgctgaagt tttaatgcca gaagggcaag ttgacgcttc agatatacat gatcacaaaa
     2641 tagacttaat taattttaat ataaaaaaat tacgattaac aaatattaaa gcctttaaac
     2701 atgatgcgac aaaaccttat gataaaacat acgataagat acttgttgat gcaccatgta
     2761 gcggattagg tgttatgaga cacaagccag agattaaata tactcaaagc aaacaacata
     2821 ttgagtcact agttgattta cagcttgaaa tattggaaaa tgtaaaaaac aatgtaaaaa
     2881 caggtggcga aatcgtctat tcaacatgta caatagagca actagaaaat gaaaacgtga
     2941 tttatacgtt tttgaaaaat aataaaaact tcgaatttga accgtttcaa catccgataa
     3001 ctggagagtt ggtcaaaacg ttacaaatca tgccgcaaga ctttaattca gatggattct
     3061 ttatcactaa gataaaaaga aaggacaatt aggaatgata actgctgaaa agaaaaagaa
     3121 gaataaattt cttccaaatt ttgacaagca atcaatatat tcattgcgat ttgacgaaat
     3181 gcaaaactgg ctcgttgaac aaggtcaaca aaaatttcga gcgaaacaga tttttgaatg
     3241 gttatatcaa aaaagagtag attcgattga tgaaatgacg aacttatcga aagacttacg
     3301 acagctttta aaagataact ttactgttac aactttaaca actgtagtaa aacaagaaag
     3361 taaagacggt acaattaaat tcttatttga attacaagat ggctatacaa ttgaaactgt
     3421 tttaatgaga catgattatg gaaattcagt ctgtgtaacg acacaagtag gttgtcgaat
     3481 cggatgtacg ttttgtgctt ctacacttgg aggcttaaaa agaaaccttg aagctggcga
     3541 aattgtttca caagttttaa cagttcaaaa agcccttgat gctacagaag agcgcgtatc
     3601 tcaaattgtc ataatgggta tcggtgaacc atttgaaaat tatgatgaaa tgatggactt
     3661 tttaagaatc gtcaatgatg ataacagttt aaatattggt gcacgtcaca ttacagtatc
     3721 aacatcaggt atcattccta gaatatatga ctttgcggac gaagatattc aaattaattt
     3781 tgctgtaagc ttacacgccg caaaagatga agtgcgatca cgcttgatgc caattaaccg
     3841 tgcatataat gttgagaagt taatcgaagc aattcaatat tatcaagaaa aaacaaatcg
     3901 tcgtgttact tttgaatatg gtctgtttgg tggtgtgaat gaccaactag aacatgcaag
     3961 agaattagcg catttaataa aaggcttaaa ctgccatgtt aacttaattc ctgtcaacca
     4021 tgttccagaa agaaattatg taaaaacggc taaaaatgat atctttaaat ttgaaaaaga
     4081 attaaagaga ctaggaatta atgccacaat acgtcgtgaa caaggttcag atattgacgc
     4141 agcttgtggt caattaagag caaaggaacg acaagtagaa acgaggtaaa gacaaatgct
     4201 agaggcacaa ttttttactg atactggaca acatagagat aagaatgaag atgcgggtgg
     4261 tattttttat aatcaaacta atcaacaact tttagttctg tgtgatggta tgggcggcca
     4321 taaagcagga gaagttgcaa gtaaatttgt tacagatgag ttgaaatccc gttttgaagc
     4381 ggaaaatctt atagaacaac atcaagctga aaattggttg cgtaataata taaaagatat
     4441 aaattttcag ttatatcact atgcacaaga aaatgcagaa tataaaggta tgggtacaac
     4501 atgtgtttgt gcacttgttt ttgaaaaatc agttgtgata gcaaatgtcg gtgattctag
     4561 agcctatgtt attaatagtc gacaaattga acaaattact agtgatcact catttgttaa
     4621 tcatcttgtt ttaacgggtc aaattacgcc ggaagaagca tttacacatc cacaacgtaa
     4681 tattattacg aaggtgatgg gcacagataa acgtgtgagt ccagatttgt ttattaagcg
     4741 attaaatttt tatgattatt tattattaaa ttcagatgga ttaactgatt atgttaaaga
     4801 caatgaaatt aagcgtttgt tagtaaaaga aggtacaata gaagatcatg gtgatcaatt
     4861 aatgcaattg gcattagata accattcgaa agataacgtt actttcatac tcgcggctat
     4921 tgaaggtgat aaagtatgat aggtaaaata ataaatgaac gatataaaat tgtagataag
     4981 cttggcggcg gtggcatgag taccgtttat cttgcagaag atacgatact taacattaaa
     5041 gttgcaatta aggcgatttt tataccacct agagaaaaag aagaaacatt aaaacgtttt
     5101 gaacgagaag tacataactc atcacagcta tcacatcaaa atatagtaag tatgatcgat
     5161 gttgatgaag aagatgactg ttactactta gtaatggaat atattgaagg tccgactttg
     5221 tctgagtata tagaaagtca tgggccatta agtgttgaca cggcgattaa ttttacgaat
     5281 caaatattgg atggcattaa acatgcacat gatatgcgta ttgtacatag agatattaag
     5341 ccacaaaata tattaattga cagcaataaa acgctgaaaa tatttgattt tggaattgct
     5401 aaagctttaa gtgagacgtc tttaactcag actaatcatg tgttaggtac tgtgcagtac
     5461 ttttcaccag aacaagcaaa aggtgaggca acggatgaat gtacagatat ttattctata
     5521 ggtattgtgt tatatgaaat gcttgttggt gaaccaccat ttaatggaga aactgcagtt
     5581 agcattgcga ttaaacatat tcaggattct gtgccaaatg tgacaacaga tgtacgtaag
     5641 gatattccgc aatctttaag taatgtcatt ttacgtgcta cagaaaaaga caaagcaaat
     5701 cgttacaaaa caattcaaga aatgaaagat gatttgagta gtgttttaca tgaaaatcga
     5761 gcgaatgaag atgtctatga actcgataaa atgaaaacga tagcggtacc tttgaaaaaa
     5821 gaagatctag caaagcatat tagtgaacat aagtcgaatc aacctaaacg tgaaacgacg
     5881 caagtaccta ttgtaaatgg gcctgctcat catcagcaat tccaaaagcc agaaggtacg
     5941 gtgtacgaac caacacctaa aaagaaatca acacgaaaga ttgtgctctt atcactaatc
     6001 ttttcgttgt taatgattgc acttgtttct tttgtggcaa tggcaatgtt tggtaataaa
     6061 tacgaagaga cacctgatgt aatcgggaaa actgtaaaag aagcagagca aatattcaat
     6121 aaaaataacc tgaaattggg taaaatttct agaagttata gtgataaata tcctgaaaat
     6181 gaaattatta agacaactcc taatacaggt gaacgtgttg aacgtggtga cagcgttgat
     6241 gttgttatat caaaaggccc tgaaaaggtt aaaatgccaa atgtcattgg tttacctaag
     6301 gaggaagcct tgcagaaatt aaaatcgtta ggtcttaaag atattgcgat tgaaaaagta
     6361 tataataatc aagcaccaaa aggatacatt gcaaatcaaa gtgtaaccgc aaatactgaa
     6421 atcgctattc atgattctaa tattaaacta tatgaatctt taggcattaa gcaagtttat
     6481 gtagaagact ttgaacataa atcctttagc aaagctaaaa aagccataga agaaaaagga
     6541 tttaaagttg aaagtaaaga agagtatagt gacgatattg atgagggtga tgtaatttct
     6601 caatctccta aagggaaatc agtagatgag gggtcaacga tttcatttgt tgtttctaaa
     6661 ggtaaaaaaa gcgattcatc agatgtcaaa acgacaactg aatcggtaga tgtaccatac
     6721 actggtaaaa atgataagtc acaaaaagtt aaagtttata ttaaagataa agataatgac
     6781 ggttcaactg aaaaaggtag tttcgacatt actagtgatc aacgtataga cattccttta
     6841 agaattgaaa aaggaaaaac agcaagttat attgttaaag tagacggtaa aactgtagct
     6901 gaaaaagaag tcagctatga tgatatttaa atataattga agtaaatgta ccgaggtttc
     6961 tatttggaag tctcggtatt tttatattga gattgcggta gttttaaaat gcttcatgtc
     7021 atcatataca ggtgaggatt gtctatcaat tctgtttcac tcccacaata tttataccat
     7081 ttgaaatgat catttatata ttaattttcc agtctttgat ataataaatg cagtaaaatt
     7141 aatcgagagg tgccattttg aagacaggtc gaatagtgaa atcaattagt ggtgtatatc
     7201 aagtagacgt taatggcgaa cgtttcaata caaaaccacg aggattattt agaaagaaaa
     7261 aattttcacc ggtagttgga gatatagtgg aatttgatgt acaaaacatt aacgaaggct
     7321 atatccatca agtgtataag cggaaaaatg agttgaaaag accacctgta agtaatatag
     7381 atacacttgt aattgtgatg agtgctgtcg agccaaattt ttcaacacaa ttattagatc
     7441 gatttttagt tattgcacat tcgtaccagt taaatgcgag agttttggtg actaaaaaag
     7501 ataaaacacc aattgaaaag cagcttgaaa ttaatgagtt gttgaaaata tatgaaaata
     7561 ttggctatga gactgaattt attggaaatg atgatgatcg aaaaaaaatt gtagaagctt
     7621 ggccagcagg acttatagta cttagtggtc aatcaggtgt cggtaagtcc actttcttaa
     7681 atcattatcg tccagaactt aatcttgaga caaatgatat atcaaaatca ttaaatcgag
     7741 gaaagcatac tacaagacat gtcgaactat tcgaacgtca aaacggttat attgcagaca
     7801 cacctggatt cagtgcttta gattttgatc acatagataa agatgaaata aaagattatt
     7861 ttcttgaatt aaatcgatat ggtgaaacat gtaagtttag aaattgtaat catatcaaag
     7921 aacctaattg taatgttaag catcaattag agatagggaa tattgcgcaa tttagatacg
     7981 accattattt acaactattt aatgaaattt caaatagaaa ggttagatat taaatgacaa
     8041 aactatatcc atcattatta tctgttgatt ttttggattt acaacatgaa ttaaaacgac
     8101 ttgaagaagc aggtgtcgac ggggttcatt ttgatgttat ggatggtcaa tttgtgccta
     8161 atatatctat tggtttacca atattagatg cagtaagaaa aggcacaaca ttacctatag
     8221 acgtacattt gatgattgaa aatccagaaa agtatattgc atcatttgca gaacatggtg
     8281 ccgatatgat ttcaattcat gtcgaatcaa cgcctcatat ccatcgtgct attcaaatga
     8341 ttaaacattt agataaaaaa gctggtgtag taattaatcc tggcacaccg atatcacaaa
     8401 ttgaacctat tttagacatt gttgattatg tactagtgat gacagttaac ccagggtttg
     8461 gtggccaatc atttattgat caatgcgtag aaaaaatagc gggtcttaat gctattaaaa
     8521 tggaacgtca attaaacttt gatattgaag ttgatggagg cgtaaatacc gatacagcga
     8581 aagtttgtgt tgaaaatggt gctacaatgc tagtaacagg ttcatttttc tttaaacaag
     8641 aggattataa aaaagtcaca caagcattga aaggttgagt gtatatgcat ataaatttat
     8701 tatgttctga tcgacacttg ccgcaagata tttgggccaa aagtaatgaa ggtaaatggg
     8761 gcggtgttga tagaggtgct ttgattttat tgaagcatca aattattcct tttttctcag
     8821 tgggagactt tgattcagtc agtaaagaag aacgccaact tctaacagaa cagttacaaa
     8881 tcaaacctgt tcaagctgaa aaagctgata cggatttagc tttagcggtt gataaagctg
     8941 ttgcacttgg atttgatagt attacaattt atggtgcaac aggcggacga ttagatcact
     9001 tttttggggc aattcagtta ttattgaaaa aagcatatta taaacatgat gttcatatag
     9061 aagttatcga tcaacaaaat aaaattgaat tattgcctaa aggtcaacat acagttgaaa
     9121 aagataagag ttatccgtac atttcattta taccgatgac tgatgatgta gaactttctc
     9181 tagcaggttt taaatataat ttagctagac aaatgcttaa tataggttct actttaacta
     9241 tttcaaatga aattgagtct ttgcaagcga aagtaattgt acatgatggg ttgattttgc
     9301 aaattagaag tacagattta aattaagtat attcaaattt gtgaagaatt ttaataaagt
     9361 gcatgagtta tttaataaag tgacgtttat agatagaatt tatagcatta taatagatga
     9421 atcacgctgt aattaatgaa gattatggcg tgatttatta ttgaaaggaa aattgtaggt
     9481 cctagtacat tggtttatgt gccaaaatcg atattgaacc cataagtcca atttgacaga
     9541 gaaccattat taaacgtttt aaccaaatta tattgatatt tcgaatttca ctatgaacca
     9601 ttatatattg ttttgtaaca aaaaaagaac taagaggttg agctcttagt tcttaagtaa
     9661 atgcgtcctc cacataaggt cacatttttt atgtgcaata tatttattaa actctagtta
     9721 ctttaccaga ttttaaagca cgtgcagaaa cccaaacttt tttaggttta ccgtcaacta
     9781 ggattctaac tttttgaagg ttagcgttcc atctacgttt agtagagttt aaagcgtgtg
     9841 aacgtctgtt accagtcgaa gctttacgac ctgttacgaa acattgttta cccatatgag
     9901 tacctccttt aataaatata aatacacata actacttata tacttaatta agatagcata
     9961 gtttcatttg aaaaacaatg aataattttc acataaaagt caaaaatact gagttttgtg
    10021 atataattgt agactgtgaa gttatgtagt atgatatttt agagaaataa gtgaatgaaa
    10081 catttaaact ttttatttaa tgatactaca tctattaaaa gaaaatgcct atagataatc
    10141 gtcatgagtg taaattcagt ttactcaaaa tataataggt gatttaagcg taagtcattc
    10201 tgataagttg taccattgat attttataaa aataatcaat gaaacttaaa gcgtttatga
    10261 ctacactaag tggtaaaatt ttaaaagtga ttagggaaaa cgaataataa actttgaaat
    10321 ttaggaggga caaagatatg acattagaga tttcaaatga ttacggtaaa attgatattt
    10381 caaacgaagt gattgcttcg gttgtaggtg gaaaggccgt tgaatgttat ggtattgtag
    10441 gtatggcatc tagacaacaa gttagagatg gtattgcgga aatactagga catgaaaatt
    10501 atgctaaagg catcaaagta actgaaaata atggcgtagt ggatatagat atgtacatta
    10561 ttgtaagtta cggtgtgaaa atatctgaag ttgccaataa tgtacaatca acagtgaaat
    10621 atactttgga aaaatcactt aatgtatcag taaattcaat caatatatat gtacaaggtg
    10681 tacgtgtgaa taatacaggc aagaaagctt aggaggacaa cttgaaatga ttagcaaaat
    10741 taatggtaaa ttatttgccg atatgattat acaaggggca caaaatttat ctaacaatgc
    10801 agatttggta gattctttga atgtgtatcc agtgccagat ggtgatacag gaacaaatat
    10861 gaatcttact atgacttcag gtcgcgaaga agtagagaat aatttgtcga aaaatatcgg
    10921 cgaattaggt aaaacattct cgaaaggttt actaatgggt gcaagaggta actctggtgt
    10981 catcttgtca caattattca gaggattttg taaaaatatt gaaagtgaat ctgaaattaa
    11041 tttaaaattg ttagctgaaa gttttcaagc tggtgttgaa acggcatata aagctgttat
    11101 gaaaccagtt gaaggtacaa tacttacagt tgcaaaagat gctgcgcaag ctgcagtaga
    11161 aaaagcaaat aatactgaag attgtataga attaatggag tacattattg taaaagccaa
    11221 tgaatcactt gaaaacacac caaacttatt agctgtactt aaagaagttg gtgttgttga
    11281 tagtggcggt aaaggtttgt tatgcgttta cgaaggattc ttaaaagcgc ttaaaggtga
    11341 aaaagttgaa gccaaagttg caaagataga taaagatgaa tttgtacatg atgaacatga
    11401 tttccatggt gtaattaata ctgaagatat tatttatggc tattgtactg aaatgatggt
    11461 tcgttttgga aagaataaaa aagcctttga tgaacaagaa ttcaggcaag atatgagtca
    11521 atttggtgat tctttattag tcattaatga tgaagaaatt gtgaaagttc acgtgcatac
    11581 cgaataccca ggtaaagtgt ttaattatgg tcaacaatat ggtgaattaa ttaaacttaa
    11641 ggttgaaaat atgagagaac agcaccgtga agtgattcga aaagaacagc acacagctaa
    11701 accgaaaatg gaaacggttg aaacagcaat tattactatt tctatgggtg aaggtatttc
    11761 agagatattt aaatcaatgg gtgccacaca tatcattagt ggtggacaaa cgatgaatcc
    11821 ttctacagaa gatatcgtta aagtcattga acaatcaaaa tgtaaacgtg caattatttt
    11881 accgaataat aaaaatatct taatggcaag tgaacaagca gcgagtattg ttgatgcaga
    11941 agcagttgtt attccaacga aatctattcc tcaaggtata agcgcactat tccaatatga
    12001 tgtggacgca acacttgaag aaaataaagc acaaatggct gattcagtaa ataacgttaa
    12061 atctggttca ttaacgtacg ctgttcgtga tacgaaaatt gatggcgttg agattaaaaa
    12121 agacgcgttt atgggcttga ttgaagataa gattgtaagc agccaaagtg atcaattaac
    12181 aacggttact gagttgttaa atgagatgtt agcagaagat agtgaaatat tgactgtgat
    12241 tattggtcaa gatgcagagc aagcagttac agataacatg ataaactgga tcgaagagca
    12301 atatccagat gtagaagtgg aagttcatga aggtggacaa ccaatttatc aatatttctt
    12361 ttcagtagaa taaaaattta aaataaaaaa ctaccaatga taaatcatca gttggtagtt
    12421 ttttattttg ctattttagt gagatagcgg gttaaaagta tcattctcga gttgctaaca
    12481 atgtcatgtt caacttagtc atgataaaat aaataacata ctaaatgata cgtaaaatca
    12541 aataaaacat aggtgattta ttttggcgaa agtaaactta atagaaagtc catattctct
    12601 tttacaatta aaaggtatag gtcctaagaa aatagaagta ttgcaacaac taaatattca
    12661 tacagtggaa gatcttgttc tttatttgcc aactagatat gaagataata cagtgattga
    12721 tttgaatcaa gcagaagatc aatctaacgt tacgatagaa ggacaagtat atacagctcc
    12781 agttgttgca ttttttggaa gaaataaatc aaaattaact gttcatttaa tggtaaataa
    12841 tattgctgtc aaatgtattt ttttcaatca accgtattta aaaaagaaaa tcgaattaaa
    12901 tcaaactata actgttaaag gtaagtggaa tagggttaaa caggaaatta ctggtaatag
    12961 ggttttcttt agttcgcaag gtacacaaac tcaagaaaac gcagatgttc aattagaacc
    13021 agtctatcgt attaaggaag gtattaaaca aaagcaaata cgagaccaaa ttagacaagc
    13081 gttaaatgat gtgacaattc atgaatggtt aactgatgga ctaagagaaa aatataaatt
    13141 agagaccttg gactttactt tgaacacatt acatcatcct aaaagtaaag agggtttatt
    13201 acgtgctcgt agaacctatg catttactga actgttttta ttcgaattac gtatgcaatg
    13261 gctaaataga ttagaaaagt catctgacga agcaattgaa attgattatg acttagacca
    13321 agttaaatca tttattgatc gtttaccttt tgaactaact gaagcacaga aatccagtgt
    13381 taatgaaatt tttagagatt taaaagcacc aatacgtatg catcgattac ttcaaggtga
    13441 tgtaggttca ggaaaaacag ttgttgctgc aatttgtatg tatgcgttaa aaacggctgg
    13501 ttatcaatca gcattgatgg taccaactga aattttagca gagcaacatg ctgaaagttt
    13561 aattgcttta tttggagatt ctatgaacgt tgcgttgtta actgggtcag taaaaggtaa
    13621 gaaacgaaaa atacttttag aacaacttga aaatggtacg attgattgtt taattggaac
    13681 ccatgcattg attcaagatg atgtgatttt ccataatgtt ggtttagtaa ttacagatga
    13741 acaacatcga tttggtgtga atcaacgcca gcttttaaga gaaaaaggtg caatgacgaa
    13801 tgtgttattt atgacagcaa ccccgatacc aagaacacta gcaatatcag tttttggtga
    13861 gatggatgtg tcttcaatta aacaattacc aaaaggtcgt aaacctatca ttactacttg
    13921 ggcaaagcat gagcaatacg ataaagtttt gatgcaaatg acctcagagt tgaaaaaagg
    13981 tcgtcaagca tatgtcattt gcccgcttat agaaagttct gagcatctcg aagatgttca
    14041 aaatgttgtc gcattgtacg agtctttaca acagaattat ggtgtttccc gtgtagggtt
    14101 attgcatggt aagttatctg ccgatgaaaa agatgaagtt atgcaaaagt ttagcaatca
    14161 tgagatagat gttttagttt ctactactgt tgttgaagta ggtgttaatg taccgaatgc
    14221 aacttttatg atgatttatg atgcggatcg ctttggatta tcaactttac atcagttacg
    14281 cggtcgtgta ggtagaagtg accagcaaag ttactgtgtt ttaattgcat cccctaaaac
    14341 agaaacagga attgaaagaa tgacaattat gacacaaaca acggatggat ttgaattgag
    14401 tgaacgagac ttagaaatgc gtggtcctgg agatttcttt ggtgttaaac aaagtggatt
    14461 gccagatttc ttagttgcca atttagttga agattatcgt atgttagaag ttgctcgtga
    14521 tgaagcagct gaacttattc aatctggcgt attctttgaa aatacgtatc aacatttacg
    14581 tcattttgtt gaagaaaatt tattacatcg tagttttgac taattgccat gctgatttgt
    14641 caatttgagt gcaacacttc gttaattgag tgatatgaca cttgaactat ttaaatgtat
    14701 agtggtattt taacaattta taaattttcg actaaataat agctaaataa tacagttatt
    14761 tgttgagtcg gttaaataga aagtgttatg atatgtgagg aatgtttaag actaggtact
    14821 aaaaaatgag gggtgagacg ttgaaactaa agaaagataa acgtagagaa gcaatcagac
    14881 aacaaattga tagcaatccc ttcatcacag accatgaact aagcgactta tttcaagtga
    14941 gtatacaaac aattcgttta gatcgcactt atttaaacat accagaatta aggaagcgta
    15001 ttaaattagt tgctgaaaaa aattatgacc aaataagttc tattgaagaa caagaattta
    15061 ttggtgattt gattcaagtc aatccaaatg ttaaagcgca atcaatttta gatattacat
    15121 cggattctgt ttttcataaa actggaattg cgcgtggtca tgtgctgttt gctcaggcaa
    15181 attcattatg tgttgcgcta attaagcaac caacagtttt aactcatgag agtagcattc
    15241 aatttattga aaaagtaaaa ttaaatgata cggtaagagc agaagcacga gttgtaaatc
    15301 aaactgcaaa acattattac gtcgaagtaa agtcatatgt taaacataca ttagttttca
    15361 aaggaaattt taaaatgttt tatgataagc gaggataaaa ttatggttaa attagcaatt
    15421 gatatgatgg gtggcgacaa tgcgcctgat atcgtattag aagccgtaca aaaggctgtt
    15481 gaagacttta aagatctaga aattatactt ttcggtgacg aaaaaaagta taatctgaac
    15541 catgaacgaa tcgaatttag acattgttct gaaaagattg aaatggaaga tgagcctgtt
    15601 agagcgatta aacgtaaaaa agatagctca atggtaaaaa tggctgaagc tgtgaaatct
    15661 ggtgaagcag atggatgtgt gtcagcaggt aatactggtg ctttaatgtc agctggttta
    15721 ttcattgttg gacgtattaa aggtgtagct agaccggctt tagtagtaac attgccaacg
    15781 attgatggaa aaggttttgt ctttttagac gttggtgcaa atgctgatgc taaacctgaa
    15841 cacttattac agtatgcgca actaggggat atttatgctc aaaaaatcag aggtattgat
    15901 aatccgaaaa tctcattatt aaatataggt accgagccag ctaaaggtaa tagtttaacg
    15961 aaaaaatcat atgagttatt aaatcaagat cattcattga attttgttgg gaatattgaa
    16021 gcgaagacat taatggatgg cgatacagat gttgtagtta ccgatggcta tactgggaac
    16081 atggtcctta aaaatttaga aggtactgca aaatcaatcg gtaaaatgtt aaaagatacg
    16141 attatgagta gtactaaaaa taaattagca ggtgcaatat tgaagaaaga tttagcagaa
    16201 ttcgctaaaa agatggatta ctcagaatac ggtggttccg tattattagg attggaaggt
    16261 accgtagtta aagcacacgg tagttcaaat gctaaagctt tttattctgc aattagacaa
    16321 gcaaaaatcg caggagagca aaatattgta caaacaatga aagagactgt aggtgaatca
    16381 aatgagtaaa acagcaatta tttttccggg acaaggtgcc caaaaagttg gtatggcaca
    16441 agatttatat aacaacaacg atcaagcaac tgaaatttta acttcagcag caaacacatt
    16501 agactttgat attttagaga caatgtttac tgacgaagaa ggtaaattgg gtgaaactga
    16561 aaacacgcaa ccagctttat tgacgcatag ttcggcatta ttagcagcac taaaaaattt
    16621 gaatcccgat tttactatgg ggcatagttt aggtgaatat tcaagtttag ttgcagctga
    16681 cgtattatca tttgaagatg cagttaaaat tgttagaaaa cgtggtcaat taatggcgca
    16741 agcatttcct tctggtgtag gaagcatggc tgcagtattg ggcttagatt ttgataaagt
    16801 cgatgaaatt tgtaagtcat tatcatatga tgacaaaata attgaaccag caaacattaa
    16861 ttgcccaggt caaattgttg tttcaggtca caaagcttta attgatgagc tagtagaaaa
    16921 aggtaaatca ttaggtgcaa aacgtgtcat gactttagca gtatctggcc cattccattc
    16981 atcgctaatg aaagtgattg aagaagattt ttcaagttat attaatcaat ttgaatggcg
    17041 tgatgctaag tttcctgtag ttcaaaatgt aaatgcgcaa ggtgaaactg acaaagaagt
    17101 aattaaatct aatatggtca aacaattata ttcaccagta caattcatta actcaacaga
    17161 atggctaata gaccaaggtg ttgatcattt tattgaaatt ggtcctggaa aagttttatc
    17221 tggcttaatt aaaaaaataa atagagatgt taagttaaca tcaattcaaa ctttagaaga
    17281 tgtgaaagga tggaatgaaa atgactaaga gtgctttagt aacaggtgca tcaagaggaa
    17341 ttggacgtag tattgcgtta caattagcag aagaaggata taatgtagca gtaaactatg
    17401 caggcagcaa agagaaagct gaagcagtag tcgaagaaat caaagctaaa ggtgttgaca
    17461 gttttgcgat tcaagcaaat gttgccgatg ctgatgaagt taaagcaatg attaaagaag
    17521 tagttagcca atttggttct ttagatgttt tagtaaataa tgcaggtatt actcgcgata
    17581 atttattaat gcgtatgaaa gaacaagagt gggatgatgt tattgacaca aacttaaaag
    17641 gtgtatttaa ctgtatccaa aaagcaacac cacaaatgtt aagacaacgt agtggtgcta
    17701 tcattaattt atcaagtgtt gttggagcag taggtaatcc gggacaagca aactatgttg
    17761 caacaaaagc aggtgttatt ggtttaacta aatctgcggc gcgtgaatta gcatctcgtg
    17821 gtatcactgt aaatgcagtt gcaccaggtt ttattgtttc tgatatgaca gatgctttaa
    17881 gtgatgagct taaagaacaa atgttgactc aaattccgtt agcacgtttt ggtcaagaca
    17941 cagatattgc taatacagta gcgttcttag catcagacaa agcaaaatat attacaggtc
    18001 aaacaatcca tgtaaatggt ggaatgtaca tgtaatatat ttgagctaaa gctcattgac
    18061 gcagtggttg actggtcatc caatggagaa atgcctgacc tagtcaactt tgcgggggaa
    18121 attctaagca accaagataa ggttccagaa tttctcccta agaaacacta atcaataaat
    18181 tgataactaa taagcggaac gcctgagcta aaactcattg acgcagtggt tgactggtca
    18241 tccaatggag aattgcctga cctagtcaac tttgcggggg aaattct
//
LOCUS       DAPIDM010000039        18150 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000039 DAPIDM010000000
VERSION     DAPIDM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.039"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            49..1230
                     /locus_tag="RJE42_001214"
     CDS             49..1230
                     /locus_tag="RJE42_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876644.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMVLLVFALILLMVFKENNTQPKKID
                     A"
     gene            1518..3935
                     /locus_tag="RJE42_001215"
     CDS             1518..3935
                     /locus_tag="RJE42_001215"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV5876645.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNAEGKVEVFTTRPDTIYGASFLVLSPEHALVDSITTDEYK
                     DQVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            3957..4268
                     /locus_tag="RJE42_001216"
     CDS             3957..4268
                     /locus_tag="RJE42_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV5876646.1"
                     /translation="MKSITTDELKTKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLDVFNKNETYYIVCAGGVRSAKVVDYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            4592..11140
                     /gene="sasC"
                     /locus_tag="RJE42_001217"
     CDS             4592..11140
                     /gene="sasC"
                     /locus_tag="RJE42_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDV5876647.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDHNV
                     QGGSNQALPGNSPNTNADTNRDIVNGSQNTPNAHATDNTSTNQALTNHQNVGVANQVA
                     PAPIQPSTSSASNNNHSDANSTATEPAANTNNNLASNNNTLNVPNNTDNNDSARHLTL
                     KEIQEDVRHSSDKPELVAIAEEASNRPKKRSRRAAPADPNATPADPTAGNGSAPVAIT
                     APFTPTTDPNANNIGQNAPNEVLTFDDNNIRPSTNRSVPTVTVVDNLPGYTLINGGKV
                     GVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIKGNDTNDHGGFNGIEKTLTVNPNSE
                     LIFEFNTMTTKNYQGMTNLIIKNADNDTVIGEKVVAYGPIWRLLKVPENVSHLKIQFV
                     PKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNKEFTVTTS
                     LKNNGNFGASFNTDDFVYKIQLPEGVEYVNNSLTKDFPSGNSGVDINDMNVTYDAANR
                     IITIKSTGGGTGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDILTYKTYTQDFIN
                     SPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNELKRRAQTILDE
                     NRNNVPLNKRVSQADIDSLVNQMQHTLIRSVDAENAVNRKVDDMEDLVNQNDELTDEE
                     KQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKQAIRD
                     KAAKQREIINNTPDATQDEIQDALNQLTTDETDAIDNVTNATTNADVETAKNNGINTI
                     GAVAPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHALEQIN
                     QATTNDDVDTAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDATQEER
                     QAAIEKVNAAVAAANTNILNANTNADVEQVKTNAIQGIQAIEPATKVKTDAKNAIDQS
                     AETQHNAIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGTQNIV
                     VIQPATQVKTDARNAVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALNDIGV
                     TSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLTDLATAKKQEINQNTNATDEEKQ
                     VALNQVDQDLATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPTALAQINQHY
                     NAKLAEINATPDATDDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAENNIDA
                     VQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAFNQINQN
                     QTNDQVDATTNQAINAIDNVEAKVVIKPKAIADIEKAVKEKQQQIDNSLDSTDNEKEV
                     ALLALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKINQKAN
                     ELRAQINQDKEATAEERQAALDKINDLVAKAMTNITNDRTNQQVNDSTNQALDDIALV
                     TPDHIVRATARDAVKQQYEAKKHEIEQAEHATDEEKQVALNQLANNEKRALQNIDQAI
                     ANNDVKRVESNGIATLKGVEPHIVVKPEAQEAIKASADNQVESIKDTPHATTDELDEA
                     NQQINDTLKQGQQDIDNTTQDAAVNDVRNQTIKAIEQIKPKVRRKRAALDNIDESNNN
                     QLDAIRNTLDTTQDERNVAIAALNKIVNAIKNDIAQNKTNAEVDQTEADGNNNIKVIL
                     PKVQVKPAARQSVSAKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQINHADK
                     TAQVNQNSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQNAAI
                     VQVEKELIKAKQQIASAVTNADVAYLLHDGKNEIREIEPVINKKATAREQLTTLFNDK
                     KLAIEANVQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIHDLDV
                     HPIKKPDAEKTINDDLARVTHLVQNYRKVSDRNKADALKAITALKLQMDEELKTARTN
                     ADVDAVLKRFNVALGDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKVKALI
                     DQYVADGIRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVLPKVGQPAPKLCTSI
                     KKVDKQEVRKVVKELPNTGSEEMDLPLKELALITGAALLARRRNKNEKES"
     gene            complement(11257..12525)
                     /locus_tag="RJE42_001218"
     CDS             complement(11257..12525)
                     /locus_tag="RJE42_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5876648.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSNVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIENQHVTVKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDTFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            12929..14590
                     /locus_tag="RJE42_001219"
     CDS             12929..14590
                     /locus_tag="RJE42_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDV5876649.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVPYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGVPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAVLSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVEAVFFIANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            14587..15282
                     /locus_tag="RJE42_001220"
     CDS             14587..15282
                     /locus_tag="RJE42_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDV5876650.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEEDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            15299..15721
                     /locus_tag="RJE42_001221"
     CDS             15299..15721
                     /locus_tag="RJE42_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDV5876651.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            15927..17336
                     /gene="sapep"
                     /locus_tag="RJE42_001222"
     CDS             15927..17336
                     /gene="sapep"
                     /locus_tag="RJE42_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDV5876652.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDVIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAKAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            17340..>18150
                     /gene="dat"
                     /locus_tag="RJE42_001223"
     CDS             17340..>18150
                     /gene="dat"
                     /locus_tag="RJE42_001223"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDV5876653.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQ"
BASE COUNT         6664 a         2726 c         3425 g         5335 t
ORIGIN      
        1 tatagtgttt attatagttt gacaaaaaag agagaggaac tatgaaatat gaatatacct
       61 aaatcagtct ggtggctagt aattggcatg gcgttaaata ttactggttc cagttttttg
      121 tggcctttaa atacaattta tatgaaacaa gaacttggaa aaagtttaac tgttgctggt
      181 ttagtgctaa tgataaattc atttggcatg gttattggaa acttattagg tggttcacta
      241 tttgataaat taggtggata caagacgatt ttaattggaa ctttcacttg tctttgtagt
      301 acaacgctac ttaatttctt tcacgggtgg ccttggtatg ctgtatggct tgtaatgtta
      361 ggctttggtg gcggaatgat tattcctgcg atatacgcaa tggctggagc agtgtggccg
      421 aatggcggta gacaaacgtt taatgcgata tacttagcgc aaaatattgg tgttgctgtc
      481 ggcgctgcaa tgggcggctt tgtcgcagaa tttagcttta actatatctt tttagccaat
      541 cttattatgt atgttgtgtt tgcacttgtc gcggtaacac aatttaatat tgaaattaat
      601 gcgaaagtta aatatccaac tcatttagat attactggta aaaagaataa agcaagattt
      661 atttcattag tactaatttg tgcaatgttt gcaatttgtt gggttgcata tattcaatgg
      721 gagtctacaa tcgcatcatt tacacaatct attaatattt caatggcaca atatagtgtt
      781 ttatggacaa ttaacggaat aatgatttta gtagcacaac cattaattaa accgattctc
      841 tatctgttaa aaggaaactt aaagaagcaa atgtttgtcg gcatcatcat ttttatgttg
      901 tcgttctttg tcacgagttt tgccgaaaac tttacaatat ttgttgtcgg tatgattatt
      961 ttaacttttg gagaaatgtt tgtatggcct gcagttccaa ctatagccaa tcagttagcg
     1021 ccagatggta agcaaggaca gtaccaaggt tttgtgaatt cagctgctac agtaggaaaa
     1081 gcattcggtc catttcttgg tggtgtatta gttgatgcat ttaatatgcg catgatgttt
     1141 atcggtatga tggtactact tgtatttgca ttaatattat taatggtttt caaggagaat
     1201 aatacgcaac ctaaaaaaat agatgcataa tgagtaaata gaattaacgt tatagacttg
     1261 aaataaatgt cgttataaca taatattaat ttgtataatt taatttcgtt tggagctttt
     1321 ctacagaaag ctagtgatgc tgagagctag tgttaaggac taaatgtaaa tcgtattaat
     1381 tttaaattga atgaatgaca tctcttacta ttaaaatgag tgcacaattt ttgtgaaata
     1441 gggtggtaac gcggcaaatg tcgtccctat gtaaatagaa tagttagagg tgtctttttt
     1501 attgaatagg aggaaatgtg ttgaattaca accacaatca aattgaaaag aaatggcaag
     1561 actattggga cgaaaataaa acatttaaaa caaatgataa cttaggtcaa aagaaatttt
     1621 atgctttaga catgtttcca tatccatcag gtgctggttt acatgttgga catcctgagg
     1681 gctatacagc aacagatatc atttcaagat ataagagaat gcaaggatat aatgtgttac
     1741 atccgatggg gtgggatgca ttcggattac cagcagagca atatgcttta gacactggca
     1801 acgacccacg tgaatttaca aagaaaaata tccaaacttt taaacgacaa attaaagaat
     1861 tagggttcag ctatgattgg gatcgtgaag ttaatacaac ggatccagaa tactataaat
     1921 ggacacagtg gattttcata cagttatata acaaaggttt agcatacgtt gatgaagttg
     1981 cagttaactg gtgtccggca ttaggcactg ttttatctaa cgaagaagtg attgatggtg
     2041 tctctgaacg tggtggacat ccagtatatc gtaagccgat gaaacaatgg gtacttaaaa
     2101 tcacagaata tgcagatcaa ttattagcgg atttagatga tttagattgg cctgaatctt
     2161 taaaagatat gcagcgcaat tggattggac gttctgaagg ggccaaagtt tcatttgatg
     2221 tagataatgc ggaaggaaaa gtagaagtat ttacgactag accagataca atctatggtg
     2281 catcattctt agtcttaagt cctgagcatg cattagttga ttcaattaca actgatgaat
     2341 ataaagatca agtgaaagcg tatcaaacag aagcttctaa aaagtcagat ttagaacgta
     2401 cagatttagc aaaagataaa tcaggtgtat tcactggtgc ttatgcaatt aatcctttat
     2461 ctggtgaaaa agtacaaatt tggattgctg attatgtatt atcaacatat ggtactggtg
     2521 caattatggc agtaccagcg catgatgaca gagattatga atttgctaaa aagtttgatt
     2581 tgccaatcat tgaagtcatt gaaggtggaa atgttgaaga agcggcatac actggtgaag
     2641 gtaaacatat taattctggt gaacttgatg gtttagaaaa tgaagcggca attactaaag
     2701 ctattcaatt attagagcaa aaaggtgctg gcgaaaagaa agttaattac aaattaagag
     2761 attggttatt cagtcgtcag cgttattggg gcgaaccaat tcctgtcatt cattgggaag
     2821 atggaacaat gacaactgtt cctgaagaag aactaccatt gttgttacct gaaacagatg
     2881 aaatcaagcc atcagggact ggtgagtctc cactagctaa tattgattca tttgtaaatg
     2941 ttgtagatga aaaaacaggt atgaaaggac gtcgtgaaac aaatacgatg ccacaatggg
     3001 caggtagttg ttggtattat ttacgttaca tcgatcctaa gaatgaaaat atgttagcag
     3061 atcctgaaaa attaaaacat tggttacctg ttgatttata tatcggtggc gtagaacatg
     3121 cggttcttca cttattgtat gcaagatttt ggcataaagt actttatgat ttaggtatcg
     3181 tacctacgaa agaaccattc caaaaattat ttaaccaagg tatgatttta ggagaaggta
     3241 atgagaagat gagtaaatct aaaggaaatg taatcaatcc tgatgatata gtacagtctc
     3301 atggtgcaga tactttacgt ctttacgaaa tgtttatggg acctttagat gctgcaattg
     3361 catggagtga aaaaggatta gatgggtctc gtcgattctt agatcgtgta tggcgtttaa
     3421 tggtaaatga agatggaaca ttgagttcaa aaatagtaac tacaaataat aaatctttag
     3481 ataaagttta taaccaaact gttaaaaagg taacggaaga ctttgaaaca ttaggattta
     3541 atactgctat tagtcaatta atggtattta ttaatgagtg ttataaagtt gatgaagttt
     3601 ataaacctta cattgaaggc ttcgttaaaa tgttagcacc tattgcacca catatcggtg
     3661 aagaattatg gtcaaaatta ggacatgaag agtctattac gtaccaacct tggccaactt
     3721 atgacgaagc actacttgta gatgatgaag tagaaatcgt tgttcaagtt aatggtaaat
     3781 tgagagctaa aattaaaatt gctaaagata catcaaaaga agaaatgcaa gaaatagcct
     3841 tatctaatga caacgttaaa gcgagtattg aaggtaaaga catcatgaaa gtcatcgctg
     3901 ttcctcaaaa attagtcaat attgtagcta aataatgttt taaggaggac tttaaaatga
     3961 agtcaattac tacagatgaa ttaaaaacca aacttttaga atctaaacca gttcaaattg
     4021 ttgatgttcg tactgacgaa gaaacagcaa tgggatatat acctaatgca aagttaattc
     4081 caatggatac gattccagat aatcttgacg tatttaataa aaatgaaacc tactatattg
     4141 tttgtgctgg cggtgttcgt agtgccaaag ttgtcgacta tttagaagct aatggtattg
     4201 atgccgttaa tgtcgaaggc ggcatgcacg cttggggcga tgaaggtttg gaaataaaaa
     4261 gtatttaaag tagtgacata atttaaaata atattacatt tgtaatgaca ccaagtaacg
     4321 tttcggttgc ttggtgtttt ttgctatgaa ttgctttctg ttacaaaaca atctaaagcg
     4381 ttcttgttat gattttttaa gtgattaatt acaaaacgga aactaaattg taataaaata
     4441 aaactttatt ttataaaatg atgatgataa aattgagtga acttaaaatg ttgtacaaaa
     4501 taatatggtt ataaacataa tcggggagct tatattttta gcataattct aaacaacaca
     4561 gcagagaata gacaaccagg aggaaaacga aatgaatttg ttaaagaaaa ataaatatag
     4621 tattagaaaa tataaagtag ggatattctc tactttaatc gggacagttt tattactttc
     4681 aaacccaaat ggtgcacaag ctttaactac ggatcataat gtgcaaggtg gttcaaatca
     4741 agcattacct ggcaactcac caaatacaaa tgccgatact aatcgtgaca tagtaaatgg
     4801 ttcgcaaaat acaccaaatg cacatgcaac agacaataca tcaacaaatc aagcattgac
     4861 taatcatcaa aacgttggtg tggcaaatca agttgcgcca gctccaatac agcctagtac
     4921 gtcatctgca tcaaacaata atcattctga tgctaattca acagcaacag agccagcggc
     4981 gaatacaaat aataatttag catcaaataa caatacatta aacgtgccta ataatacaga
     5041 taacaatgat tcagcgcgtc atctaacttt aaaagaaatt caagaagatg ttcgtcattc
     5101 gtctgataag ccagagttag ttgcgattgc tgaagaagca tctaatagac cgaaaaagag
     5161 aagcagacgt gctgcgccag cagatcctaa tgcaactcca gcagatccaa cggcaggaaa
     5221 cggtagtgca ccagttgcaa ttacagcgcc attcacgcca acaactgatc ccaatgccaa
     5281 taatatagga caaaatgcac ctaacgaagt gcttacattt gatgataaca acattagacc
     5341 aagtacgaac cgttctgtgc ctacagtaac cgttgttgat aatttaccag gctacacact
     5401 gattaatggt ggtaaagtag gggtgtttag tcatgcaatg gtaagaacta gcatgtttga
     5461 ttcaggagat gctaagaact atcaagccca aggcaatgta attgcattgg gtcgtattaa
     5521 aggaaatgat acaaatgatc atggcggttt taatggtatc gagaaaacat taacagtaaa
     5581 tccgaattct gaattaatct ttgaatttaa tactatgact actaaaaact atcaaggtat
     5641 gacaaattta atcattaaaa atgctgataa cgatactgtt attggtgaaa aagtagttgc
     5701 ttatggtccg atttggcgct tattaaaagt acctgaaaat gttagtcatt taaaaattca
     5761 atttgtacct aaaaatgacg caataacaga tgcacgtggt atttatcaat tacgagatgg
     5821 atataaatac tatgactttg tagactcaat cggtcttcat tctgggtcac atgtctatgt
     5881 tgaaagacgt acaatggagc caacagcaac aaataataaa gaatttacag ttacaacgtc
     5941 attaaagaat aatggtaact ttggcgcttc attcaataca gatgattttg tatataaaat
     6001 tcaattacct gaaggtgttg aatatgtaaa taattcattg actaaagatt ttcctagcgg
     6061 taattcaggt gttgatatta atgatatgaa tgtgacgtat gacgcagcaa atcgaattat
     6121 tacaattaaa agtactggtg gaggtacagg gaattcgccg gcacgactta tgcctgataa
     6181 aatattggat ttgaagtata agctacgtgt gaacaatgtg ccaacaccaa gaacagtaac
     6241 atttaatgat atattaacgt ataaaacata tacacaagat tttattaatt cacctgctga
     6301 aagtcatact gtaagtacaa atccatatac tatcgatatc ataatgaata aagacgcatt
     6361 gcaagccgaa gtcgatagac gtattcaaca agcggattat acatttgcat cattagatat
     6421 ttttaatgaa cttaaaagac gcgcacaaac aattttagat gaaaaccgta acaatgtacc
     6481 tttaaacaaa agagtttctc aagcagatat cgattcatta gtaaatcaga tgcaacatac
     6541 gttaattcga agtgttgacg ctgaaaatgc cgttaataga aaagttgatg acatggaaga
     6601 tttagttaac caaaatgatg aactgacaga tgaagaaaaa caagcagcga ttcaagtcat
     6661 cgaggaacat aaaaatgaaa ttattgggaa tattggtgac caaacgactg atgatggcgt
     6721 tactagaatt aaagatcaag gtatacagac tttaagtgga gacactgcaa caccagttgt
     6781 taaaccaaat gctaaacaag ctatacgtga taaagcagcg aaacaaagag aaattatcaa
     6841 taacacacca gatgctactc aagacgaaat tcaagatgca ttaaatcaat taacaacgga
     6901 tgaaacagat gctattgata atgttacgaa tgctactacc aatgctgatg ttgaaacagc
     6961 taaaaataat ggtattaata caattggtgc agttgcgcca caagtgacac acaaacaagc
     7021 agcaagagat gcaattaacc aagcgacagc aacgaaaaga caacaaataa atagcaatag
     7081 agaagcaaca caagaagaga aaaatgcagc attgaatgaa ttaacgcaag ccacgaacca
     7141 cgcattagaa caaatcaatc aagcgacaac caatgatgat gtagatactg ccaaaggtga
     7201 tggtctgaat gccattaatc ctattgcgcc tgtaactgtt gtcaagcaag cagcaagaga
     7261 tgctgtatca catgatgcac aacagcatat cgcagagatc aatgcaaatc ctgatgcgac
     7321 tcaagaagaa agacaagcag caattgagaa agtaaatgct gctgtagctg ctgcgaatac
     7381 taatatttta aatgctaata ccaatgctga tgttgagcaa gtaaagacaa atgcaattca
     7441 aggtatacaa gccattgaac cagctacaaa ggttaaaaca gatgctaaaa acgctattga
     7501 tcaaagtgcg gaaacgcaac ataatgcgat atttaataat aatgatgcga ccttagaaga
     7561 gcaacaagca gcacaacaat tgcttgatca agctgtagcc acagcgaagc aaaatattaa
     7621 tgcagcagat acgaatcaag aagttgcaca agcaaaagat cagggcacac aaaatatagt
     7681 tgtgattcaa ccggcaacac aagttaaaac ggatgcacgc aatgctgtaa atgataaagc
     7741 gcgagaggcg ataacaaata tcaatgctac acctggcgcg actcgagaag agaaacaaga
     7801 agcgataaat cgtgtcaata cacttaaaaa tagagcatta aatgatattg gtgtgacgtc
     7861 tactactgcg atggtcaata gtattagaga cgatgcagtc aatcaaatcg gtgcagttca
     7921 accgcatgta acgaagaaac aaactgctac aggtgtatta acggacttag caactgctaa
     7981 aaagcaagaa attaatcaaa atacaaatgc aactgatgaa gaaaagcaag tagcattaaa
     8041 tcaagtagac caagatttag caacggcaat taataatata aatcaagctg atactaatgc
     8101 agaagtagat caagcgcaac aattaggtac aaaagcaatt aatgcgattc agccaaatat
     8161 tgtaaaaaaa cctacagcat tagcacaaat caatcagcat tataatgcta aattagctga
     8221 aatcaatgct acaccagatg caacagatga tgagaaaaat gctgcgatca atactttaaa
     8281 tcaagacaga caacaagcta ttgaaagtat taaacaagcg aatacaaatg cggaagtaga
     8341 ccaagctgcg acagtggcag agaataatat cgatgctgtt caagttgacg ttgtaaaaaa
     8401 acaagcagcg cgagataaaa tcactgctga agtggcgaag cgtattgaag cggttaaaca
     8461 aacacctaat gcaactgacg aagaaaagca ggctgctgtt aatcaaatca atcaacttaa
     8521 agatcaagca tttaatcaaa ttaatcaaaa ccaaacaaat gatcaggtag acgcaactac
     8581 aaatcaagcg attaatgcta tagataatgt tgaagctaaa gtagtaatta aaccaaaggc
     8641 aattgcagat attgaaaaag ctgttaaaga aaagcaacag caaattgata atagtcttga
     8701 ttcaacagat aatgagaaag aagttgcttt actagcatta gctaaagaaa aagaaaaagc
     8761 acttgcagct attgaccaag ctcaaacgaa tagtcaggtg aatcaagcgg caacaaatgg
     8821 tgtatcagcg attaaaatta ttcaacctga aacaaaagtt aaaccagcag cacgtgaaaa
     8881 aatcaatcaa aaagcgaatg aattacgtgc gcaaattaat caagataaag aagcgacagc
     8941 agaagaaaga caagcggcgt tagataaaat caatgattta gttgctaaag ctatgacaaa
     9001 tatcacgaat gatagaacaa atcagcaagt taatgactca acaaatcaag cgcttgacga
     9061 cattgcatta gtgacgcctg accatattgt tagagcaact gctagagatg cagttaagca
     9121 acaatatgaa gctaaaaagc acgaaattga gcaagcggaa catgcgactg atgaagaaaa
     9181 acaagttgct ttaaatcaat tagcgaataa tgaaaaacgt gcattacaaa acatcgatca
     9241 agcaatagcg aataatgatg tgaaacgtgt tgaatcaaat ggtattgcta cgttaaaagg
     9301 cgtagaaccg cacattgtgg ttaaacctga agctcaagaa gccataaaag cgagcgcaga
     9361 taaccaagta gaatctataa aagatacacc acatgctacg actgatgaat tagatgaagc
     9421 aaaccaacaa ataaacgaca cacttaaaca aggtcaacaa gatatagaca atacgacaca
     9481 agatgcagct gtcaatgatg ttagaaatca aacgattaag gcaatcgaac aaattaaacc
     9541 gaaagttaga cgcaaacgtg cagcgttgga taacattgat gaaagtaata ataatcaact
     9601 cgatgcaata cgaaatacgc tagatacaac gcaagatgaa cgaaatgttg ctattgctgc
     9661 gttaaataaa attgttaatg caattaaaaa tgatattgca caaaacaaaa cgaatgcaga
     9721 agtggatcaa actgaggctg atggtaacaa caacatcaaa gtgattttac ctaaagttca
     9781 agttaaacca gcagcgcgtc aatctgtcag cgcaaaagct gaagctcaaa atgcacttat
     9841 tgatcaaagt gatttatcta ccgaagaaga aagattagct gctaaacatt tagtagaaca
     9901 agcacttaat caggctattg atcagatcaa tcatgcagat aagactgcgc aagttaatca
     9961 aaatagtatc gatgctcaaa atattatttc aaaaattaaa ccagcgacaa cagttaaagc
    10021 aacagcatta caacaaattc aaaatatcgc tacaaataaa attaatttaa ttaaagcaaa
    10081 taacgaagcg acagatgaag aacaaaatgc tgcaatagta caagttgaaa aagagttaat
    10141 taaagctaaa caacaaattg ctagtgcagt gactaatgct gatgtggcat atttattgca
    10201 tgatgggaaa aacgaaattc gtgaaatcga acctgttatt aataaaaaag caactgcgcg
    10261 agaacaatta acaacattat tcaacgataa gaaactagca attgaagcga atgttcaagc
    10321 aacggtagaa gaaagaaata gtattttagc acagttacaa aacatttatg acactgctat
    10381 tggacaaatt gatcaagatc gtagcaatgc acaagttgat aaaacagcat cattaaatct
    10441 acaaacaata catgatttag atgtacatcc tattaaaaag ccagatgctg aaaaaacgat
    10501 taatgatgat cttgcacgtg ttacacattt agtgcaaaat tatcgaaaag taagtgatcg
    10561 taataaggct gatgcattaa aagctataac tgcattaaaa ttacaaatgg atgaagaatt
    10621 aaaaacagca cgcactaatg ctgatgttga tgcagtttta aaacgattta atgttgcatt
    10681 aggcgatata gaagcagtaa ttactgaaaa agaaaatagc ttactgcgca ttgataacat
    10741 tgctcaacaa acatatgcga aattcaaagc gatcgcaaca ccagaacaat tagctaaagt
    10801 aaaagcatta attgatcaat atgttgcaga tggcataaga atgattgatg aagatgcgac
    10861 attaaatgac atcaaacaac atacgcaatt cattgttgat gaaattttag caattaaatt
    10921 accagctgaa gcgacgaaag tattaccaaa agtggggcaa cctgctccaa aactttgtac
    10981 gtctattaaa aaagtagata aacaagaagt acgaaaagtt gtaaaagaac ttccaaatac
    11041 tggttctgaa gaaatggatt taccattaaa agaattagca ctaattacag gcgcagcatt
    11101 attagctaga agacgtaata aaaatgaaaa agaatcataa ttaacaaaaa gaccttaata
    11161 taacgacact ttttagtgac taagttatat taaggtcatt tttaattgaa tgaaaaggtt
    11221 gatctactag agagaatcga acccaatttt atactattat tccattgatg catgagagta
    11281 atgaccggca tataatccag cgacatgtcc tgttacgagt gcacttgtaa tattataacc
    11341 accagtataa ccatgtatat ctaatacttc accacataaa aataatcccg gaactaattt
    11401 agacatcatt gttttaggtt gaatttcttt aagtgacaca ccaccacctg tgacaaatgc
    11461 cttatctata ggtaatgtcc cgtttacctt aaatacaaac cctttaaaca tgtttactaa
    11521 gtcgttcaat tgttgatttg ataagtgatg tgatgtggta ttttcatcga tcccagcttg
    11581 ttctaacatg aacagtaagt agcgttcttc aattagtcca tgcaagctgt ttttaatgat
    11641 tttatctggt gtgtccgata ataatgatgt gatgtgttgt tttaattgtt catggtttaa
    11701 ttcaggaaat gtatcgattg ccattgaaat atgctgtgtc ttttgatttt tttgttcttt
    11761 ataaacaaac tgactacatc ttaatgcagc tggaccactg ataccaaaat gagtaaataa
    11821 catatccatt tgatgactga tgcgtttttt accatttttc ttaagtactg acaattcaac
    11881 atcttttaaa cttaaacctt ttagacgatt ggatttaatg aaaggttcag ctgatgtaat
    11941 tggaacttcg gtcgggaata actccgtaat ggtatgacct aaatcttgtg caaacttata
    12001 accatcacct gttgaaccag tttgagggac acttgtacca cctgtagcaa ttactagcga
    12061 atggctttca taactattat tttgagtatg tacagtgaaa gtttggtctg tattaacttc
    12121 gattctacta acagcttctt cttccttaac ggttacatgt tgattttcta tagttgtcac
    12181 taaagtatca acgacgtctt gtgccttatt ggaaactgga aacatacgcc cgtgatcttc
    12241 ttcttttaat ttaacacccc tagactcaaa aaaatctatg atggattcat tatcaaaaat
    12301 tgaaaaggga ctatataaaa atttcccatt tccaggaatg ttcttaataa tttcagcata
    12361 tggtaatcga ttagttacgt tacatctacc gccaccagat attttgagtt tacgacctag
    12421 acctttcttt ttttcaatga gtaacacatt gctactttgt tcgcttgcag ctactgccgc
    12481 cattaagccg ctaggtccgc ctccgataat aattgtttga tacatatacg tttcctccaa
    12541 atttgttact ggacattcaa ttgctatttt atagtacttt tttaaacttt gctatatctc
    12601 ttgtgttatg aaatgacact cacgctgaat atatttataa tgtgtttttc ataaaaggat
    12661 aagcatttat tcgaacacta ttattatttt aagagttgtc cgccatttat tggcgttagt
    12721 agtataatga caatatgaaa atttatgaat ttcaaagcaa ctttgagtat tcagtgacaa
    12781 cacgttatta tttttgttat gatatttcat tgaatatacg acaatgaaat aaagttttgt
    12841 gatatattca taataatgaa aatgatttta cagagaaata gctttttatt tacaaaacat
    12901 taataaacat gagataggga gatacgtaat gagtgaaagt aaagaaatgg tgcgtgggac
    12961 ctttttaatt acaattagta ttttgattac aaaggtgtta ggcgtacttt ttatcattcc
    13021 attcaactat ttaattggtg gacaagaaaa tatggcgccg ttcacatacg cttatgcacc
    13081 atataatatt gcaattgcag ttgcgacagc aggtgtacca ttagctgctt ctaaatatgt
    13141 agcgaagtat aatgcgattg gtgcatataa agtcagtcag aaattttata aatctagttt
    13201 tattgttatg agtattaccg gtgttttagg tttcttagta ctttatttcc tagcaccata
    13261 tatttctgaa ttaactttag ctagaaatgt tcacgataaa aatggttggt cagttgatga
    13321 cattacatgg attattagaa tcattagtat ggtagtcatc ttcattcctg tattagcaac
    13381 atggagaggt atattccaag gttataaatc tatgggacct actgcggtat cggaagtaac
    13441 tgaacaaatt gcacgcgtga ttttcatatt aatcggaagt tatttagtac taaatgtctt
    13501 tgacggatct attttgttag ccaatggtat agctacattt gctgctgctg tcggtgcgat
    13561 tattgggata ttcacacttt ggtattattg gagaaaacgt aagcataata ttgatcgcat
    13621 ggttgaatct gattataccg atatcgatgt gccgtacggc aagatgtata aagaaattat
    13681 tgcttacagt attccattcg ttatcgtaag tttgaattat ccattattta acttagtaga
    13741 tcaatttacg cataatggtg cattatcatt agttggtgtg ccatcacagt tacaagatat
    13801 attctttaat atgctgaata tgtctacaaa taaaattgtt atgattccaa cgtcattaag
    13861 tgctggtttt gcagtaagtt taattcctta tattacaaaa acatttgcag aaggtcgctt
    13921 acatgaaatg catcatcaaa ttagaacatc tattggtgta ttaatgttta taactgttcc
    13981 agctagtatt ggaattatgg ccttggcaca accattattc acagtattct atggttacga
    14041 tccaattgta ttaggtcatg accctaacca tgatggtagt cgcttattat tctattatgc
    14101 gcctgtagca atattaattt cactattaag tgtaacggcg tcaatgttac aaggtatcga
    14161 taagcagaag ttaacagttt atgttatttt agcttctgtt gtaattaaat tagcattaaa
    14221 ctacccatta attatgctat tccatacgcc gggggctgtt ttaagcacaa gtatcgcatt
    14281 attattcgca attggttgta acttctatat tcttaaaaaa tacgcgaaat ttaaatttag
    14341 ctatagctgg attcatttcg caaaaatatt cttatactca tttattatga tgttaggtgt
    14401 agaagcagta ttcttcattg cgaacctatt cttagagcca actaaacttg gttacttaat
    14461 cattattatt ttaggtgtta cagtaggtat tctaatttac ggtacaatca caattaaaac
    14521 gcgtcttgcg gacgaattct taggtgaaat tcctgaaaaa ttaagacgta gagttaggtt
    14581 tttacgatga gaatagataa atttttagca aatatgggcg tcggaacacg taatgaggtc
    14641 aaacagttac ttaaaaaagg ccttgtaaat gtcaacgaac aagtaattaa atcaccaaaa
    14701 acacatatag aaccagaaaa cgataagata tcagttcgtg gagaattaat agaatacgtt
    14761 gaaaatgtct atattatgtt aaataagcca aaaggttata tatcggcaac tgaagatcat
    14821 cattcaaaaa ccgttattga tttaatacct gaataccaac atttaaatat ttttccggtt
    14881 ggtcgtcttg ataaagatac agaagggctt ttattgataa cgaatgatgg tgattttaat
    14941 catgaattaa tgagtccaaa caaacatgtt tctaaaaaat atgaagttat ttcagcaaat
    15001 cctatcactg aagaagacat tcaagctttt aaagagggtg taacactgtc tgatggcaag
    15061 gttaagcctg caatattaac ttatattgat aatcaaacgt cacacgtaac aatttatgaa
    15121 ggaaaatatc atcaagttaa acgaatgttt catagcattc aaaatgaggt attacattta
    15181 agacgtatca aaattgctga tttagaattg gatagcaatt tagattcagg agaatatcgt
    15241 ttattaactg aaaatgattt tgacaaatta aattataaat aaaagggaga cgattgatat
    15301 ggcaaaaggt aatttattta aagcgatttt aggtataggt ggcgctgtag cagctgtact
    15361 tgttacacgt aaagatagtc gtgacaagct gaaagcagaa tataataaat acaaacaaga
    15421 tcctcaaagc tataaagata atgctaagga taaagcgacg caattaggaa caattgcaaa
    15481 tgaaacaatt aaagaagtaa aaacaaatcc gaaagaatat gctaatagat taaaaaataa
    15541 tccaaaagca tttttcgaag aagaaaaatc aaaatttacc gaatatgaca ataagactga
    15601 cgaaagtatt gaaaaaggta aatttgatga tgaaggtggc gcagcaccaa ataataattt
    15661 acgtatcgtc actgaagaag atttaaaaaa gaataaaaat gcattgtctg ataaagaata
    15721 aggcatacaa ttaataagtt ggtattgaac tggcatatta aattaaatta atacagaaaa
    15781 ggaacaaagc agttatcatt aattttatgt taaatcgata gcgttgtttt gttccttttt
    15841 attattttct gacatcttat tgtagttttt atatcatttc atgtaaaata ggatttaact
    15901 tatagaaaaa aggaagtcga tttcgaatgt ggaaagaaaa agttcaacaa tacgaagatc
    15961 aaatcattaa tgacttaaaa ggattattag caattgaaag tgtgagagat gatgcaaaag
    16021 catcagaaga cgcaccagtt ggtccaggtc ctcgtaaggc attagactac atgtatgaaa
    16081 ttgcacatag agatggattt acaacacatg atgtggatca tattgcagga agaattgagg
    16141 caggtaaagg aaatgacgta ttaggtatct tatgtcatgt tgacgttgtt cctgctggtg
    16201 atggatggga tagtaatccg tttgagccgg ttgtaacaga agatgttatc atagctagag
    16261 gtacacttga tgacaaaggt ccaacaattg ctgcttatta tgcaattaag atattggaag
    16321 atatgaatgt ggattggaag aaacgtattc atatgattat tggtacggat gaagaatctg
    16381 attggaaatg tacggatcgc tattttaaaa cagaagaaat gccaacatta ggttttgcac
    16441 cagatgcaga atttccgtgt attcatggtg aaaaaggcat tactacattt gacttagttc
    16501 aaaataagct tactgaagat caagatgaac ctgattatga attaataact tttaaatctg
    16561 gtgaacgtta caacatggta cctgatcatg cagaagcaag agtgcttgtt aaagaaaata
    16621 tgacagatgt tattcaagac tttgagtact ttttagaaca aaatcattta caaggtgata
    16681 gtactgttga tagtggcatt ctagttttaa cagttgaagg taaagcggtt catggtatgg
    16741 atccatctat cggtgtgaat gcgggtcttt acttactaaa attcttagca tcattaaatc
    16801 ttgataataa tgcaaaagcg tttgtagcat ttagtaatcg ctacttattt aattcagatt
    16861 ttggtgaaaa gatgggaatg aaattccata cagatgtcat gggtgatgtg acaactaaca
    16921 ttggtgttat tacatatgat aatgaaaatg cgggtctttt cggtatcaac ttgcgctacc
    16981 cagaaggatt tgaatttgaa aaagctatgg atcgttttgc aaatgagatt caacaatatg
    17041 gctttgaagt gaaattaggt aaagtccaac caccacatta tgttgataaa aatgatcctt
    17101 ttgtacaaaa gttagttact gcatatagaa atcaaacaaa tgatatgact gaaccttata
    17161 ctataggtgg cggtacttat gcgagaaact tagacaaggg tgtagcattt ggcgcaatgt
    17221 ttagtgattc tgaagactta atgcatcaga agaatgaata tatcactaaa aaacagttat
    17281 ttaatgcaac tagtatttat ttagaagcaa tttattcatt atgtgtggag gaataatata
    17341 tggaaaaaat ttttttaaat ggtgagtttg taagtccaag tgaagcaaag gtttcataca
    17401 acgacagagg atacgtattt ggcgatggta tttatgaata cattcgcgta tataatggta
    17461 agttatttac agtaacggaa cattatgaaa gatttttacg tagtgccaat gagattggtt
    17521 tagatttaaa ttattctgta gaagaattaa ttgaactatc tcgtaaatta gttgatatga
    17581 atcaaattga aactggggca atttatattc aagcaacgcg tggtgtagct gaaagaaatc
    17641 atagtttccc gacacctgaa gtagaaccag caattgttgc ttatactaag agttatgatc
    17701 gtccttatga tcatttagaa aatggtgtga atggtgttac cgttgaagat atccgatggt
    17761 tacgttgcga cattaaaagt ttgaacttat taggaaatgt attagcaaaa gaatatgctg
    17821 tgaaatataa tgcagttgaa gcaatccaac atcgaggcga aactgtcact gaaggatctt
    17881 caagtaatgc ttatgcaatt aaagacggtg tgatttatac acatccgatt aacaactata
    17941 ttcttaatgg tattacacga attgtaatta aaaaaattgc cgaagactat aacatcccat
    18001 ttaaagaaga aacgtttact gtagatttct tgagaaacgc agatgaagtt attgtttcaa
    18061 gtacttcagc tgaggttaca cctgttatta aattagatgg tgaaccagtt aatgatggta
    18121 aagttggccc aattacacgt caactacaag
//
LOCUS       DAPIDM010000040        17697 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000040 DAPIDM010000000
VERSION     DAPIDM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.040"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            77..694
                     /locus_tag="RJE42_001224"
     CDS             77..694
                     /locus_tag="RJE42_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDV5876654.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            822..2288
                     /locus_tag="RJE42_001225"
     CDS             822..2288
                     /locus_tag="RJE42_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDV5876655.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHAAPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAAALGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHNHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            2395..3525
                     /locus_tag="RJE42_001226"
     CDS             2395..3525
                     /locus_tag="RJE42_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5876656.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADRFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDANLFSLKGALWTLAQEVYQEWYLGSKLYEEVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNKRDKYVSYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(3486..3581)
                     /locus_tag="RJE42_001227"
     CDS             complement(3486..3581)
                     /locus_tag="RJE42_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876657.1"
                     /translation="MKPLFQSLTSSYAIKQQSNYKLSSTPLSSPK"
     gene            complement(3675..5279)
                     /locus_tag="RJE42_001228"
     CDS             complement(3675..5279)
                     /locus_tag="RJE42_001228"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV5876658.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHKQFKVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(5266..6426)
                     /locus_tag="RJE42_001229"
     CDS             complement(5266..6426)
                     /locus_tag="RJE42_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDV5876659.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(6540..6986)
                     /locus_tag="RJE42_001230"
     CDS             complement(6540..6986)
                     /locus_tag="RJE42_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV5876660.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVKGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            7066..7809
                     /locus_tag="RJE42_001231"
     CDS             7066..7809
                     /locus_tag="RJE42_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV5876661.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKFDKKLLIELKSPNLYPGIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRKYWYKKPNFSRIAQIASYVNPNYALVTRKFVDEAHHHQLQVLPY
                     TVNKLKTGEKLRHMGVDGLITDDPKLFIK"
     gene            complement(8027..8629)
                     /gene="rpsD"
                     /locus_tag="RJE42_001232"
     CDS             complement(8027..8629)
                     /gene="rpsD"
                     /locus_tag="RJE42_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV5876662.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(8873..9337)
                     /locus_tag="RJE42_001233"
     CDS             complement(8873..9337)
                     /locus_tag="RJE42_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDV5876663.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            9474..11168
                     /gene="ezrA"
                     /locus_tag="RJE42_001234"
     CDS             9474..11168
                     /gene="ezrA"
                     /locus_tag="RJE42_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDV5876664.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYGNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            11496..12644
                     /locus_tag="RJE42_001235"
     CDS             11496..12644
                     /locus_tag="RJE42_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV5876665.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     DQLLQQAKVQINDMVNTNMTYDVVFTSGATESNNIALKGVAYRKFDTAREIITSVLEH
                     PSVLEVVRYLEEREGFKVKYVDVTKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMADVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNVSFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLTAMGLSDKAIEGSIRLSFGAMTTKEDITKFKETFTIIYEEIKELLK"
     gene            12644..13867
                     /gene="thiI"
                     /locus_tag="RJE42_001236"
     CDS             12644..13867
                     /gene="thiI"
                     /locus_tag="RJE42_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV5876666.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTLEAMSATAIKFAQQFEE
                     NSTFKIDVKRADKNYPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVQGAGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGAYAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            13911..14681
                     /locus_tag="RJE42_001237"
     CDS             13911..14681
                     /locus_tag="RJE42_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDV5876667.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSAIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLIFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLVMAASMIVGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            14779..15273
                     /gene="tpx"
                     /locus_tag="RJE42_001238"
     CDS             14779..15273
                     /gene="tpx"
                     /locus_tag="RJE42_001238"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV5876668.1"
                     /translation="MTEITFKGGPIHLKGQLINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            15396..16343
                     /locus_tag="RJE42_001239"
     CDS             15396..16343
                     /locus_tag="RJE42_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV5876669.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSRDFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            16431..17633
                     /locus_tag="RJE42_001240"
     CDS             16431..17633
                     /locus_tag="RJE42_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV5876670.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVDGEKVKTVQDIKDHVEAVDIMLDAFKKHNIINDINDIDGTGHRVVHGGEKFPESA
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLQRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTYGGLK"
BASE COUNT         6061 a         2532 c         3278 g         5826 t
ORIGIN      
        1 cagactatct tgttctagta aaaatatagt gataaaatct agttgttata aacaaataag
       61 ttaagtaggt ggcacaatgt attcagtgat tagtaagatt ttgaatttca ttttagttaa
      121 aatgtcaaaa tcattatacg taatcggtaa agataatatt cctaaagata gtaaatatgt
      181 cgtaacttgt acgcatgaaa gttataacga agtcattatg ctaggtatgg cattatatcc
      241 taatcaaatt cattatatgg ctaaaaaaga attattcaaa aataaatgga ttggtaaatt
      301 tttaacgtct ttaaatgcat ttcctgtaga tcgtgaaaat ccaggaccta gtactttaaa
      361 acgcccaatc aatttgttga aagataataa gacagttggt atcttcccaa caggttctcg
      421 cacatcgcaa gaaggtgcac ctttgaaacg tggtgcttca acaattgcaa tgttaagtaa
      481 gtcaccaata ttacctgttg cttatgttgg tcctactaag attcatggtc ttttaacagg
      541 acaagcatac ataaatattg gtaaaccaat cgatattaac gatttaccta aagatttaaa
      601 acgaaacgag cgtatcgatt acattacaaa agagattgaa acacgaacag caaagttaca
      661 acaagaattg catgaaattg taaaaagttt ataaaataac ttgaggtaga aatatagctg
      721 gttagtaagc ctaggtgact ataacggagt atcgaagtag ttattctagt tacattagta
      781 atctttatat ttctacctta ttttaagaag gatgacattc aatgtataaa tttttccaaa
      841 atttagggcg ttcgttaatg ttaccagttg ccattttacc ggctgcagca attattgccg
      901 gaattggtaa tacgttaaat gcacttcatg cggcgccgaa aatcgcaatg ttctttacta
      961 cggtaggtac tacgatttta gagcaattag gtattttatt tgcaattggt gtagctatag
     1021 gaatggcaaa gaaaaatgat ggtgcagtcg cgcttgcagc tgcgttagga tattttttag
     1081 ttacagttgt attatcacca atgaaattag cgcctttatt aggaatgaaa gcttctgaaa
     1141 taaattcagc ttttgaaaag atgaataatg gcaatgtctt tgttggtatt gtaataggtt
     1201 taatagcagc ttatgcatac aataagttta gtgaaacaga attaccatta gcattatcat
     1261 tttttagtgg taaacgttta gttccaatta tgactgcatt ttactgtaca tttttagttg
     1321 tcatattgtt attcttatgg ccactacttt attcatggat tgtaaaattt ggtgaatcta
     1381 tagttggttt aggatcgttt ggtgctttca tatatggtgt tgccaacaga ttattaattc
     1441 ctacaggatt acatcatgca ctgaatagtg tgttctggtt tgatacgatt ggtattaatg
     1501 atattggtaa attccaaagt ggcaaagatg ctataaaagg tattacagga cgctatcaag
     1561 cgggattctt tccaatcatg atgttcggta ttccagcggc tgcgttagca atgtatcata
     1621 cagctaaaac tactcagaaa aaacaagttt acggttggtt cctagcgagt tcagttgcag
     1681 catttttcgt tggtgttaca gagccgattg agtttgcatt tatgtttgtt gcgccaatct
     1741 tatatgttgt tcatgcgtta ttaacaggat tatctttatt tatcgcagct acattccatt
     1801 ggacagctgg attttcattt agtgcaggtt tgatagatta tgtgttatca ctaattaatc
     1861 cggtatcaaa tcatccattg atgttgcttg ttcaaggtgt tgtatttttc atcctatact
     1921 atgtgatttt ccgtgtagta atccaagtat ttaacttgaa cacgattggt agaggtgaaa
     1981 atgaattagt tgatccaaca gttgtaaaag ataatattgc tcctggtgaa aatgatatta
     2041 aacaaagtaa atatcataat catgctatac aaatattaga aggtttaggt ggacaagaga
     2101 atattgttaa tttaaccaat tgtgcaacaa ggttgcgtct agagttaaaa gacacatcta
     2161 ttattgatca acagaagatt aaaaacgccg gtgcagttgg tgttactgtt aatggcaaac
     2221 attcaactca agttattgtc ggtacacacg tacagcaggt tgctgatgaa attgaaaaac
     2281 atttataaaa tttaaacagc taaagcgcaa atctaacttc aatagggttt gcgctttttt
     2341 tagtcaaata gtagaatgat ttatatctga attttcagaa atggagtggg gattgtgaag
     2401 aaaattttat ttgatgttga tggcgttttt ttaagtgaag aacgttgttt tgatgtctct
     2461 gcattaacgg tatatgaatt attaatggat aagtgttatt taggtcttca ttcacatata
     2521 gattgggaaa ctttgactga taacgatata caagacatta ggaatcgaat ttttcaaaaa
     2581 gataagattt taaacaaatt aaaatcgcta gggctgaatt caaattggga tatgttattt
     2641 atcgttttta gcattcattt aattgatatt ttgaaaaagt tatcacatga tgaaattgag
     2701 gcattcatgt atcaagatga gcctgtcgaa ttgaagttgc aaaatataag cacaaactta
     2761 gcagaccgtt ttaatttaaa tgaacaacta cctttacaat ttttggataa tgtaaaagtt
     2821 ggtaaaaata atatttatgc tgctttggaa gagtttgcaa caacagagtt acatgtttcg
     2881 gatgctaatt tatttagttt gaagggtgca ttatggacgt tagcgcaaga agtttatcaa
     2941 gaatggtatt taggatcgaa gttgtatgaa gaggttgaaa agaaaatagc acgaactact
     3001 tttaagacag gttatattta tcaagaaatt attttgagac cggtagatga agttaaggta
     3061 cttctgaatg atttaaaagg tgctggtttc gaattaggta tagcaacagg tcgtccttat
     3121 actgagactg ttgtgccatt tgaaaattta ggattgttac catattttga agctgatttt
     3181 attgcaacag caagtgatgt tttagaagca gagaatatgt atccgcaagc acgaccatta
     3241 ggaaagccaa atccttttag ttatatcgca gctttatatg gtaataaacg cgataaatat
     3301 gtatcttata tcaataagca agataacatt gtaaataaag atgacgtatt tatagtaggc
     3361 gattcgttag ctgacttatt aagtgctcaa aaaataggtg caacgtttat tggaacatta
     3421 acaggtttaa aaggaaagga tgctgcaggc gagttagaag cgcatcatgc cgactatgtt
     3481 attaatcatt taggtgaact tagaggtgta ctagataatt tgtaatttga ttgttgtttg
     3541 atagcatagc ttgaagttaa tgattgaaac aaaggtttca tattgagtta caatgaaatt
     3601 aataatgaaa aaatgccaag aagcaatgga agtaatccaa tgtcttcttg gcattttgaa
     3661 tttacataaa ttgtttatga ctgtaccgtc aattcagttg tgaaaatttg attgtattca
     3721 ccaacttgtt taagttcatc aattatattg tttgaaacag gttgatcaac ggataaaatc
     3781 attagcgcat ctccgcccgc ttcagttcta cctaaagtca tagatgcaat gttgatattg
     3841 tatttaccta acaatgcgcc agttttacct accatacccg gggtatcatt atgatatgac
     3901 acaatttgat attgatttgg ctttaagtct acagaaaaat tattaattct aacaattctt
     3961 ggaccgaaac ctgtaaagac agaagcgcca actttaacgg aatcgctatc gtttgatagt
     4021 tctacctcta agtagttact aaaacctgtc tctgctttat tattttcaat atttaatgtc
     4081 acttgttgtt gatttaacaa cattagagca ttgataatat tgacttcagg accaagatca
     4141 tcttttaaaa tatgagtaat aattgtacgt gttaataaac tactatcgat agaggctaag
     4201 tcgccaccgt aagtaatttt aatagagctt ggtgcattat acattaattg aatagctaat
     4261 tcaccaactg tttggcttaa attgatgaat gattttacag tatcatctat attgcttaag
     4321 tccattttag gtgcattcac tgcatgcgtt acagtaccat caattaaaat ttcgatgatt
     4381 tcatttgaaa cagaaattgc cactttttct tgagcttcga ctgttgaagc acccaaatga
     4441 ggtgtaacaa taattttatc atgtgcaaca agaggcgaat cagttgcagg ttcatgttca
     4501 aacacatcga tagctgcccg actaatttgt ccttcgtcta atgcttttat taaggccttt
     4561 tcatcaataa taccaccacg tgccacattg attatttgca aactaggttt tgctttggca
     4621 aaaaagtcag cattaattaa gccctttgtt ttaggtgtta gtggtgtatg caatgtaacg
     4681 aaatcagaat gttgggcaat ctcatcaact gttgctttcg taatgcttaa agattttgct
     4741 ttttcatccg ttaagtaagg gtcaaaagct agtattttca ttccgaaact ttgcgcacgt
     4801 ttagcaacac ctaaaccaat tctaccagca ccaatgacac ctaatgtttt atgataaagc
     4861 tcagtacctt taaatgcatt tcgattccat tctttatttg taagtgattg gtgtgcttgc
     4921 ggaatatttc gagccattga taataacatt gctagtgaat gttcagtagc tgaaatcgta
     4981 ttaccatctg gggcattaat aactaaaata ccttttaatg ttgcagcatc aatattaata
     5041 ttatctacac caacaccggc gcgtgcgatt acttttaaag aatcagcagc atttatgata
     5101 ttttcagtaa ccgtagtttg actacgaacg attaaagcat ggtatgaagg tataattttg
     5161 attaatgctt cttcggacaa gccagtttga atatctacct tgaattgttt gtgatctaat
     5221 aatgctttga taccatcttt tgatatggga tctgcaacga gtacattaaa ttgcttcatg
     5281 aataacctcc atatattttg atataccttt accgatatag ttaactttac ggtgttcagt
     5341 taaaataatt tctaaagcag atactaccga taaaatatcg aaaggactaa ttttccccat
     5401 atgaccaatt cttaaaattt gacctttaag atggccttga ccaccagcaa ttgttatttt
     5461 aaagcgattt ttaagttcat ctttgattat tttcacttca tcatttgtgt taggtttgaa
     5521 tgccgtaacc gttggagatg catctttatc atttactaat aaagttaaat ctaatgcctt
     5581 taaggcgctt cttaatgcat ttctaattgc atagtgtcgt gctattacgt gattgaaacc
     5641 ttctgctttt acggtttcaa cgtatgcatt tacacctcta aataaagaca catttggagt
     5701 gaacggtgta gaattgtcag cttgtgacga aatgtattta tttaagtcta gataaaattt
     5761 tggtgtagtt acttctttga atcgttcttt tgcacggtgg ctatacgcta caaaagctaa
     5821 tcctggaggt aacataattg ctttttgact accagaaaca agtacatcaa ttttatcttt
     5881 gttaatgtca acatcaacag caccaatgca acttacgccg tcaactacaa aataaatatt
     5941 actattaaat tgattgatgg catttcctaa ctcgtgaata gggtgtagta ctgtcgtaga
     6001 tgtttcgcaa tattgactaa atactgcttt aacttcaaca tttaaagttg aaaggtaatt
     6061 gatgaaatct ttgacatcta cagcttctcc ccatgttacg tcataaatat gtacattttt
     6121 gtaataagtt tgtgcaattt gtttaaatcg gttaccaaag gcacctgaaa caatgacaac
     6181 gaagtgatct tcagggttta caatgttcaa catactagct tctaagacgc ttgtaccgct
     6241 agatgttaat ataagtacat cattttgact cccaaatatt ggctttagac cttgaaatgc
     6301 ttgttgtgcg atgtcttcaa aatctttaga acgatgacca accataggtg cttgaatttc
     6361 tctcataatg gcatcaggta caggggttgg gccaggtgtt aataacaacg gttgatgata
     6421 atacatttat atgcctccta taaataagat ttcactattt tagcaaattt tctgaaaatt
     6481 taaaagccta aaaaagtgat aacgctatat tgtcgaacaa atgttatcac ttaacgattt
     6541 tatttggcct gaatgatggg atagattttt atgtccacat tatttcttac agcatttgaa
     6601 atcatgcaat tattatctgc aattgttatc aattttggta atcgcttttc taattgtgct
     6661 atttgatcac ttggaatttg aatgtgagga tggtgcacaa tttttgacat actgaatttt
     6721 ccattattta aacaagctgt tccaatcgat tgttgttcaa ttgaaatatc tgtgaacttt
     6781 gcacgttcaa gagtagctgc taatgagatg atataacatg atgaagcggc tgatactaac
     6841 atttcatcgg gatttgttcc aataccaaca ccgcctaaag aagcaggtat tgaaatattc
     6901 tctgaaagta tgtcgccttt aacgttcccg acgttgttac gaccaccttg ccaagaagtt
     6961 tggactttaa agtcatgttg atgcaattgc tttaacctcc aatataattg tgataattta
     7021 attttagaat agtttatcaa agtttaacag aaaggtgact tatcaatgac tctgaataaa
     7081 ctgaaagatg aattacaaat tgtttcgcat cgtggattgc cgagtgattt tcctgaaaat
     7141 acaatggtcg gttatcgaga ggtaatgggg ctcaatgttg ctatgttaga aatagatgtt
     7201 catttgacca aagaccaaca ttttgttgtg atacatgatg aaacaattga tagaacatcg
     7261 gatggtaagg ggcgtattgc tgattacaca ttatcgcaat taaaatcatt tgattttggt
     7321 agttataaag atgttgcttt taagggagaa cgaataccca cgttagatga agtactttca
     7381 ttatgcttaa agtttgataa aaagctattg attgaattaa aaagtccgaa tttatatcca
     7441 ggaatagaat gtaaattgct ggcattttta gaagaaaaga aagtcgatgc gacacaagta
     7501 gttatacaat cttttgatat cgaatgcatc gaaaagttaa acacattagg tagtatatat
     7561 gaattaggtg tgctttgtag taaacgtaag tattggtata aaaagccaaa cttttcaaga
     7621 attgctcaaa ttgctagtta tgttaatcct aattatgcat tagtgaccag aaaatttgtt
     7681 gatgaagcac atcatcatca attacaggtt ttgccataca cagttaataa attgaaaact
     7741 ggagaaaaat taagacatat gggtgtagat ggattaatta cagatgatcc gaaattattt
     7801 attaagtaag acgatgaatg gttatttgat agagggataa gggttcgtat atcgcaattg
     7861 ataattttaa aaactaatga ggaaatatta tagataattc aacaaataag acttgtaaat
     7921 tctaagttga ttgtaaaata gggtagctcc atataatctt gaatctatca tttttaaatt
     7981 aatataaaaa aacacccaca attgtgggtg tttgtattga acggtattat cttgagtagt
     8041 actcaacgat taattgttcg ttaatttcag caggtaattc gctacgttct ggtaaacgta
     8101 cgaaagtacc agttaagctg tcagcatcaa agtttaagta ctcaggtacg aaattgttga
     8161 tttcaactga ttcaacgatg atgtttaatt tttgagattt ttcacgaact gaaattgttt
     8221 gaccaggttt aacagaataa gatggaatat caacacgttt accatctact aagatatgac
     8281 cgtggttaac taattgacgt gcttgacgac gagtacgagc taaacctaat gaataaacaa
     8341 cagcgtctaa acgacttgct aataagatca tgaagttttc accgtgtaca ccgaattttt
     8401 taccagcgat gtcaaatgtg ttacggaatt gtctttcagt cattccatat aagtaacgta
     8461 atttttgttt ttcacgtaat tgtaaaccat attctgataa ttttttacgt tggtttggac
     8521 catgttgtcc tggtgcgtaa ggacgttttt ctaattcttt accagtaccg cttaaagaga
     8581 tacctaaacg acgagatttt ttccagtttg aacctctgaa tcgagccata ataagactcc
     8641 tcctttaatt cttttttgtt gttatgaata aacaaaaaag agtgttgcat gcttactaag
     8701 atatattgtt ttatgtgtct tcaccttata gcgacagtta cgtgacacgt ccacattggg
     8761 aacaacatag cgctcaataa tatacaactg ctactttcgt taattcacac aaagtttatt
     8821 gtaacatttt cctgctaact gtcaacactt tattgaaaat gctgatgtcc ttttatgcga
     8881 gttgcttttc aataatttta acaattgctt caagatgttc cttgtcattg tcatcaaatc
     8941 gatccgttat aggcgcatcg atatctaaga caccgataat tttatcatct ttgaaaattg
     9001 gaacgacaat ttcagactta ctattagcat cacaagcgat atgtccttcg aattgatgga
     9061 catctgctac aacttgtgta cgacgttctg aaacggctgt accacataca ccttttccaa
     9121 ttggaatgtg gacacaagcg gggtgtcctt ggaaaggtcc aagtataagt tcattttgtt
     9181 ccaataagta aaagccgacc caattaattt gatctagatt atcatttaat aaggcagaca
     9241 tgttacttaa aatagcaatc atgtgatgtt catcttcaat aaggcttgct gcttgtttct
     9301 ttaataatgt gtagtttgtt gggttaattg ttgtcatatt taaaacctct tattttcata
     9361 gattttataa tagtatagaa tttcgaaaat attatggcaa taaattcatt ttataattta
     9421 tccttaagca aaattacgtt ataatagtaa tgataaatta ggaggagaag catatggtgt
     9481 tatatatcat tttggcaata attgtgatta tattgattgc tgtaggtgta ttattctatt
     9541 tacgttcaaa taaacgacaa attattgaaa aagcaatcga acgtaaaaat gaaattgaga
     9601 cgttaccttt tgatcaaaac cttgcacaat tatctaagtt gaatttaaaa ggtgaaacaa
     9661 aaacgaaata cgatgcaatg aaaaaggaca acgtagaaag tacaaataag tatctagctc
     9721 ctgtggaaga aaaaatccat aatgctgagg ctttattaga taaatttagt ttcaacgcat
     9781 ctcaaagtga aattgatgat gcaaatgagt tgatggatag ttacgaacaa agctatcagc
     9841 aacaattaga agatgtaaat gaaattattg cgttatacaa agataatgat gaattatatg
     9901 acaaatgtaa ggttgattat cgtgaaatga aacgtgatgt tttagcaaat cgtcatcaat
     9961 ttggtgaggc agcaagtctt cttgaaactg aaattgaaaa attcgagcca aggttagagc
    10021 aatatgaagt actaaaagct gatggtaatt atgtacaagc gcacaaccat atagctgcct
    10081 tgaatgaaca aatgaaacag ctaagatctt atatggaaga aataccagaa ttaattagag
    10141 aaactcaaaa agaattacct ggtcaattcc aagatttaaa atatggttgc cgtgatctta
    10201 aagttgaagg gtatgatctg gatcacgtaa aagtagacag tacattacaa agcttaaaaa
    10261 cagagcttag tttcgttgaa ccattaatta gtcgcttaga attagaagaa gctaatgata
    10321 aactagctaa tatcaatgat aagttagatg acatgtatga tttaattgaa catgaagtta
    10381 aagctaaaaa tgatgtcgaa gaaacaaaag atatcattac ggataactta ttcaaagcta
    10441 aagacatgaa ttatacattg caaacagaaa ttgaatatgt acgtgaaaac tactatataa
    10501 atgaatctga tgctcagagt gttcgtcaat ttgaaaatga aattcaaagt ttaatttctg
    10561 tatatgatga tattttaaaa gaaatgtcta aatctgctgt acgatatagc gaggttcagg
    10621 ataatttaca atatttagaa gatcatgtca cagttattaa tgacaaacaa gaaaagctac
    10681 aaaatcacct gattcaattg cgtgaagatg aagcagaagc agaagacaat ctgttacgag
    10741 tacaatcgaa gaaagaagaa gtgtatcgtc gattacttgc ttctaactta acaagcgttc
    10801 ctgaaaggtt tatcatcatg aaaaatgaaa ttgatcatga agttcgtgat gttaacgaac
    10861 aatttagtga acgtccaata cacgttaaac agttaaaaga taaagtgtct aaaattgtga
    10921 ttcaaatgaa tacatttgaa gatgaagcaa atgatgttct tgttaatgct gtttatgcag
    10981 agaaattaat tcaatatgga aatagatatc gtaaggacta tggcaatgtt gataagagct
    11041 taaatgaagc tgaacgatta tttaaaaata atcgctataa gcgtgcgatt gaaattgcag
    11101 agcaagctct tgaaagtgtt gagccaggtg tcactaaaca tattgaagaa gaagttatta
    11161 agcaatagca tttattatac ttaatcgtat tttacgaagt gataggagtt agccaactgt
    11221 atgttgtcta gcttcttttt tatatggata tatgataagg agattcaaat tagcaatcgt
    11281 ttaaaaataa atgtcattca atgtgattgt tgaaataatg caggtagcga aaatgcgtcg
    11341 ttccacagct gtatgcatag agtgaaatag ttcaattctt attaaaatgg gtggaaaaat
    11401 gattaatttt tgttatagcc tttatttttt atgtgataat agtataatgc ttgagtaaaa
    11461 acaattttaa aagattgatg attaatgaaa gagaagtgtt tattttgata tatctagata
    11521 atgcggcaac gacgaaagca tttgaagaag tgttagatac ttatttaaaa gtaaatcaat
    11581 caatgtatta taatccgaat agtccgcata aagctggttt gcaagcagat caattattac
    11641 agcaagcaaa agttcaaata aatgatatgg tcaatactaa tatgacatat gatgttgttt
    11701 tcactagtgg tgcaacggaa tctaacaata tcgcgcttaa aggtgtggct tatcgtaaat
    11761 ttgatacagc aagagaaata attacatctg ttttagaaca tccgtctgtt ttagaagttg
    11821 tcagatattt ggaagagcgc gaaggcttta aagttaaata tgttgatgta acaaaagatg
    11881 gcagtattaa cttagaacac ttcaaagaat taatgtcaga caaagtcggt ttagtaacat
    11941 gtatgtatgt aaataatgta acaggacaaa tacagcctat tccacaaatg gcagatgtta
    12001 taaaaaatta tcctaaggca cattttcatg tagatgcggt tcaagcattc ggcaaaattt
    12061 caatggattt caataacgta gatagtatta gtttaagcgg acacaagttt aatggtttaa
    12121 aaggtcaagg cgtcttactt gtaaatcaca ttcaaaatgt tgaaccaact gtccatggtg
    12181 gtggtcaaga atatggcgtt agaagtggaa cagttaattt gccaaatgat attgcaatgg
    12241 ttaaagcgat gaagatagct aatgaaaacc ttgaagcatt gaatgcattt gttactgagt
    12301 taaataatga cgtccgtcaa tttttaaata aatatcatgg agtttatatt aattcttcaa
    12361 cttcaggttc accattcgtt ttaaacgtta gttttcctgg tgtaaaaggt gaagtgttag
    12421 tcaatgcatt ttcaaaatac gatattatga tttctacaac aagtgcttgc tcatcaaaac
    12481 gtaataaact aaatgaagta ttaactgcaa tgggattatc agataaagcg attgaaggca
    12541 gtattcgact gtcatttggt gctatgacga cgaaagaaga tataacaaaa tttaaagaga
    12601 catttacgat aatatatgag gaaattaagg agttgctaaa ataatgaagt atgatcactt
    12661 gcttgttaga tacggggaat taacactaaa aggttcaaat agaaagaaat ttgtgaatca
    12721 attaagaaat aatgtaaata agtcattaaa aggacttgat gggtttgtcg ttaaaggcaa
    12781 acgagatcgt atgtatattg aacttgaaga ccatgcagat ataaatgaaa taacatatcg
    12841 attatcaaaa attttcggta ttaaatctat tagtccggta ttaaaagtag aaaaaacatt
    12901 agaggcaatg agtgcaacgg caattaaatt tgcgcagcaa tttgaagaaa acagcacatt
    12961 taaaattgat gtaaagcgtg ctgacaaaaa ttatccaatg gatacgtatg aattacagcg
    13021 tgaattgggt ggtgcagtat tgaagcactt cgacaatatt tcagtgaatg ttaaacgtcc
    13081 agatcatgaa attcgagtgg aagttagatt agatgcaatt tatatgtatg aagaagttgt
    13141 tcagggcgca ggcggattac cagttggtac tggtggtaag acgttactaa tgctttcagg
    13201 cggtatagac tcaccagttg ctgggatgga agtgatgaga cgtggcgtaa caattgaagc
    13261 gattcatttc catagtccac catttacaag tgatcaagca aaagaaaaag ttattgaatt
    13321 gacacgtatt ttagctgaac gtgttggacc aattaaattg catattgtac catttacaga
    13381 attgcaaaaa caggtaaata aagttgtaca tccaagatat acaatgactt caacgagacg
    13441 tatgatgatg cgtgttgctg ataaattagt acatcaaata ggcgcttatg ccatcgttaa
    13501 tggtgaaaat ctaggccaag ttgccagtca aacacttcat agcatgtatg caattaataa
    13561 tgtaacttct acccctgtat tacgtccttt attaacttac gataaagaag aaattattat
    13621 taaatcgaaa gaaattggta catttgaaac atctattcaa ccatttgaag attgttgtac
    13681 aattttcacc cctaaaaatc cagtaaccga accaaacttt gataaggtag ttcaatacga
    13741 aagtgtcttt gattttgaag agatgattaa tcgtgctgtt gaaaatattg aaacacttga
    13801 aataacaagt gattataaaa ctattaaaga acagcaaaca aaccaattaa taaacgactt
    13861 tttataaata aaatcctaga gtaaatttaa acataagggg atgttaaact atggatttga
    13921 atttaacgat gattataatc ataattttat ttggttttat cgcggcgttt atagattcgg
    13981 ttgtaggagg tggcggttta atttctacgc cagcattatt agcaatcggt ctaccaccat
    14041 ctgtggcttt aggtacaaat aaattggcaa gttcatttgg ttctttaact agtgcgataa
    14101 agtttataag gtccggtaaa gtggacttat atgttgttgc caaattattt ggttttgtat
    14161 ttttggcatc tgcatgtggc gcatatattg caaccatggt tccgtcacaa atattgaaac
    14221 ctttaatcat tattgcactt tcgtcggtgt ttattttcac attacttaaa aaagattggg
    14281 gcaatacacg tacgtttact caatttacat ttaagaaagc catactattt gcagcacttt
    14341 ttattttaat cggcttttat gatggatttg taggtggagg aacaggttca tttatgcttt
    14401 ttgtattgtt aatctttggt tttgattttt taagcgctgc aggaaatgct aaagttttga
    14461 attttgcttc taatataggt gcgcttgtat tatttatggt attaggacaa gtagattatg
    14521 taataggttt agttatggct gcgagtatga ttgttgggtc atatgctggg gctcattttg
    14581 ctattaaaca aggtgttggc tatgtaaaag tattatttat tattattact gcgattttaa
    14641 ttttaaaaaa tgcttttgat tatattcaac aatttgtata aaattaaatt tgcttttgat
    14701 taaagcatga aaattgtaat caaaccataa attgtcgtat gatgtagtta gaattttaaa
    14761 atgcaggagg tcaagtatat gactgaaata acattcaaag gtggaccaat ccacttaaaa
    14821 ggtcaactaa ttaatgaagg tgattttgca cctgatttta cagtgttaga taatgactta
    14881 aatcaagtaa cattagcaga ttatgctggt aaaaagaaat taattagtgt ggtaccatca
    14941 attgatacag gtgtttgtga tcagcagact cgcaaattca actctgaagc ttctaaagaa
    15001 gaggggattg tgcttacaat ctcagcagac ttaccattcg cacaaaaaag atggtgcgct
    15061 tcagcaggtt tagacaatgt cattacatta agtgaccacc gtgacttatc atttggtgaa
    15121 aactatggcg ttgttatgga agagcttcgc ttattagctc gtgcagtatt tgtattagat
    15181 gcagataata aagttgttta taaagaaatc gttagtgaag gtactgattt cccagatttt
    15241 gatgctgctt tagctgcata caaaaatatt taatcattaa agagataaat cttaaaatgt
    15301 atacatcgtg tccatcgttg tcaacagcat taaaatagaa ttgttttcta tgattgctaa
    15361 gacctatggg cactttttat tggagaggga cgaatatggc agaacaacaa acaattatgg
    15421 aacgcttgtt tcatacatta gatgaaaaag ctaaaacatt aaataatgaa aatggccaaa
    15481 gttttattga aaatcttggg ctagcaatgg aacaagttta tacaaatgaa agaggattgt
    15541 tagaacaatc gacgttacaa gatcgacgta aagcattcca atttgcatat ttaagtttaa
    15601 tgcaggaaga aaagatacaa gcaaatcatc aaattacacc agattcaatt ggattgatat
    15661 taggattttt agttgagcga tttatgaaca accaagaaga attacatatt gttgatattg
    15721 caagtggtgc tggtcattta agtgctactg taaaagaagt gttacctgaa attgcggtta
    15781 tgcatcattt aattgaagtt gatccagttt tatcacgtgt tagtgtacat ttagcaaact
    15841 tcttagaaat tcctttcgat gtgtatcctc aagatgccat tatgccacta ccgttagaag
    15901 aagcagatat cgttattggt gattttccag taggctatta tccaattgat gaaagaagca
    15961 gggactttaa gctaggtttc gaagaaggac atagttattc acattattta ttaatagaac
    16021 aagcaataaa tgcattaaaa gatgctggat atgcctttct agtggtacca agtaatattt
    16081 ttacaggtga acatgtaaaa cagcttgaaa aatatattgc aacagagaca gagatgcaag
    16141 catttttaaa tttaccacca actttattta aaaatgaaaa agcgcgaaaa tctatattaa
    16201 ttttacaaaa gaaaaaatcg ggtgaaacaa agccagttga agtattattg gcaaatattc
    16261 ctgatttcaa aaatccttca caatttcaag gatttatgac agagttaaat cagtggatgg
    16321 acacaaatcg tcctaaaaaa taatctgtaa tagtatagtc ataaaactgt atgaatgata
    16381 aaatgaaaaa tgaaatagta taaaaagcaa acgatggagg atcattactt atgtcaaaat
    16441 taatcttggc tatcaacgct ggtagttcat cattaaagtt tcaattaatc agaatgcctg
    16501 aagaggaatt agtaacaaag ggattagtag aaagaatcgg attaaaagat tcaattttta
    16561 caattgaagt agacggggaa aaagttaaaa cagtacaaga tatcaaggat catgttgaag
    16621 cagttgatat catgttagac gcatttaaaa aacataatat cattaatgat attaacgata
    16681 tcgatggaac tggtcaccgt gttgtacatg gtggtgaaaa attccctgaa tcagcagcaa
    16741 ttactgatga agttgaaaaa gaaattgaag aattaagcga attagcaccg cttcacaacc
    16801 cagctaactt aatgggtatt cgtgctttcc gtaaattatt accgaatatc cctcatgtgg
    16861 cgatttttga tacagcattc catcaaacaa tgcctgaaaa agcatattta tatagcttgc
    16921 catatcatta ttataaagat tacggtattc gtaagtatgg cttccatggt acaagtcata
    16981 aatttgtgtc acaaagagcg gcagaaatgt tagataaacc aattgaagat ttacgcatta
    17041 tctcttgtca tattggtaac ggtgcatcaa tcgcagctat cgatggcggt aaatcaattg
    17101 atacatcaat gggcttcaca ccacttgcag gtgtaacaat gggtacgcgt tcaggtaata
    17161 ttgaccctgc tttaattcca tttattatgg aaaaaactgg caaaactgct gaacaagtat
    17221 tagaaatctt aaacaaagaa tctggtttat taggattatc aggtacatca agtgatttac
    17281 gtgacttatc tgaagaagct gaatcaggca aagcacgttc tcaaatggct ttagatgtct
    17341 ttgcttctaa aatccacaaa tatattggat catacgctgc aagaatgcat ggcgttgatg
    17401 taatcgtatt tactgcaggt attggtgaaa attcagtgga aattcgtgca aaagttcttg
    17461 aaggtttaga atttatggga gtttactggg atcctaagaa aaatgaaaac ttacaacgtg
    17521 gtaaagaagg atttattaac tatccgcatt cacctgttaa agttgtcgtt ataccgactg
    17581 acgaagaaag tatgattgct cgtgatgtta tgacatacgg tggtctaaaa taatttttta
    17641 ataaatatta agcgtctatc tccgctacaa ttataagtag gaggtagacg ctttttg
//
LOCUS       DAPIDM010000041        17328 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000041 DAPIDM010000000
VERSION     DAPIDM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.041"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            284..1084
                     /locus_tag="RJE42_001241"
     CDS             284..1084
                     /locus_tag="RJE42_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5876671.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKSRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDFLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(1330..2322)
                     /locus_tag="RJE42_001242"
     CDS             complement(1330..2322)
                     /locus_tag="RJE42_001242"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV5876672.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSVTVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            2592..3263
                     /locus_tag="RJE42_001243"
     CDS             2592..3263
                     /locus_tag="RJE42_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDV5876673.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            complement(3383..4189)
                     /locus_tag="RJE42_001244"
     CDS             complement(3383..4189)
                     /locus_tag="RJE42_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5876674.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLPVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(4364..4648)
                     /locus_tag="RJE42_001245"
     CDS             complement(4364..4648)
                     /locus_tag="RJE42_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV5876675.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            4875..5309
                     /locus_tag="RJE42_001246"
     CDS             4875..5309
                     /locus_tag="RJE42_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5876676.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            5387..6355
                     /locus_tag="RJE42_001247"
     CDS             5387..6355
                     /locus_tag="RJE42_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDV5876677.1"
                     /translation="MTNNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALIYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHAAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHD"
     gene            6377..6970
                     /locus_tag="RJE42_001248"
     CDS             6377..6970
                     /locus_tag="RJE42_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5876678.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPIEITSTKNLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAQVEEVWVNGHEITETGLTAGQQILEK"
     gene            complement(7075..8154)
                     /locus_tag="RJE42_001249"
     CDS             complement(7075..8154)
                     /locus_tag="RJE42_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876679.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(8499..10463)
                     /locus_tag="RJE42_001250"
     CDS             complement(8499..10463)
                     /locus_tag="RJE42_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDV5876680.1"
                     /translation="MTQITERELKKKYLDLLSQHFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKDKLSTKELNDLTALVYYPEDKLQL
                     IKCDFQNYGQLNVWYITTIEHLIQLIKYCSSKYTRSKLRRALPEQYVFIVEELLYKNN
                     EFKNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIVEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPVIKRRP
                     NFEMDERLVLEKVNYDTNEITVYGKTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPCNGNLLIHGCIPVDENGEMESFEIDGQTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRIVDKELQ
                     RKKIRNTNKGKELQAQIDILKMLMHDRYLD"
     gene            complement(10837..11607)
                     /locus_tag="RJE42_001251"
     CDS             complement(10837..11607)
                     /locus_tag="RJE42_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876681.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            complement(11609..12304)
                     /locus_tag="RJE42_001252"
     CDS             complement(11609..12304)
                     /locus_tag="RJE42_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV5876682.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKENDLE
                     NVLLEIIEREDQA"
     gene            12744..13205
                     /locus_tag="RJE42_001253"
     CDS             12744..13205
                     /locus_tag="RJE42_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5876683.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKGLVVESHNEFNKREVNYSLTQEGKK
                     LSYIHDELHEKAVKKYEEVLKVFDNDEMAVIVEFLNRSIEELKKEEDGLND"
     gene            13198..14361
                     /locus_tag="RJE42_001254"
     CDS             13198..14361
                     /locus_tag="RJE42_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876684.1"
                     /translation="MTKSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSIKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPTLAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAMFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLATIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIIIFLYIKIGDFQLKYAK"
     gene            14351..14489
                     /locus_tag="RJE42_001255"
                     /pseudo
     CDS             14351..14489
                     /locus_tag="RJE42_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14459..15070)
                     /locus_tag="RJE42_001256"
     CDS             complement(14459..15070)
                     /locus_tag="RJE42_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV5876685.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENILPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            15617..16894
                     /locus_tag="RJE42_001257"
     CDS             15617..16894
                     /locus_tag="RJE42_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876686.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
BASE COUNT         5977 a         2571 c         3068 g         5712 t
ORIGIN      
        1 agacaggcgt ttgggaaaca cgtaaaatct ttgtagctac tgaagttaaa taatccatta
       61 cgctaatgga tgaatatatt gagtggaaaa cagtcttgtt tgcgagactg ttttttgttt
      121 ggtgtgaggt gaaaagtaat ttaatatcca gtgtaatgtg ttttgcatat atgaaagcaa
      181 tttcaaaatg tgaatatagg ttcattgtgg tatgacaaac ttcattgctt gtcatgagat
      241 ggatataatg aagacgacta aaagagcaat gaggaggata atcatggtta aggctattgc
      301 ggtagatatg gacgggacgt ttttagattc gaaaaagaca tacgataaat caagatttga
      361 agcgattttt actgaactta gaaatagaga tattacattt attgctgcga gtggcaatca
      421 atatgcgaag ttgaagtcta ttttcgggga tagagatatg tattttattt ctgaaaatgg
      481 tgcagttatt tataaaggca atgagttata taattataaa agctttaatc gtcaggtgtt
      541 tcaacaggtt gtcgatttct taaatatgaa ccaaagtatt gatcaactcg tcatctgtgg
      601 tttgaaaagc gcgtatattt taaaacatac ttctgaagcg tttaaagaag atacgagatt
      661 ttattatcat cagttaaaag aaattgacag tctacaacaa ttacctgagg atgattatgt
      721 caaaatagca ttcaatatta atcgtgagac gcatccaaat gtagacgaag aagtagcgac
      781 gcaattcagc aatgatatta aacttgtctc aagtgggcac gatagtatag atattattat
      841 gccaaacatg actaaaggac aggcattaaa acgattgtta gataaatggg aaatgtctcc
      901 ttcagagctc atggccttcg gagatgcgaa taatgataaa gatatgctag cgttcgctaa
      961 gcatagttat gtgatggaaa atagtcatga tgaagaattg ttcaatattg cctctgctgt
     1021 cgctccaagt aatgataagc agggtgtttt aacgattatc gaacaagaag tgttgaaaaa
     1081 gtaggtagta tatcatcaat gaagcagcta tcatgatact aaatacatgt taaatgaaaa
     1141 ctttgaatac gaatcatcaa atattaatat atctcaaaca ggttaggcaa taatgccata
     1201 gtaaaataag cgcactacaa tcatttgaat agatgataaa ataaagcgga ttttaatcaa
     1261 atcgattttg atacagatat acaaaaaaaa agcaaaggca catggcgcgc ctttgctttt
     1321 aaatatatat taatttaaac gtgtttcaca tgtaccagtg ttaatgacag ataatgctgc
     1381 gtttaaacca ccttcaacaa ggttttgtac tgcttcatca gagaagaaag caatatgtgg
     1441 tgttactaaa attctttcat gttcgattaa ctctaataat gttttatcgt caatgtcttt
     1501 attagtccag tcatttgtga agtatgctgc ttcattttca taagtatcaa tcgcagcacc
     1561 taacaaagta ccatcgttca ctgcagcgat taaatcaggt gtattgatga ctgcaccacg
     1621 tgctgcgtta actaagattg cacctttttt cacatgatca aacatagctt tatcgaataa
     1681 atgatagctt tctttgttcg ctggaacatg taaagaaata atatcagcat ctttaatagc
     1741 ttctttaaca ctatctttgt atgttaaaaa gtctaaatct ttattaggat aagcgtcata
     1801 agctgtaatt gtagcaccaa atcctgcata tattttagct gtagcagcac cgatacgacc
     1861 cgtaccgata attgcaacag tcatattttt aactggttta gacatgattt ctgcttgcca
     1921 agtaaaatca tgtgcttgta cacggcgttc aatatctggg aagcgacgca ctaattgtag
     1981 ggcgatagaa acagaatact ctgcaattgt ttcaggtgaa taactaggaa cgttagatat
     2041 cacaatattg tgttttttag ctaaatctaa atcatacata tcaaatccag cagtacgttg
     2101 tgcaatttgt ttaataccgt aagattctaa tttaggataa acgtcatttt ctaacttacc
     2161 aaattgcatt gtagttacgc catcgtaatc ttttaattga tcgactgtaa cacttgataa
     2221 tagctcttta gaagtagtta cttcgacatt attctttttc ccccaattta atgccatctc
     2281 tttctcataa tcacgcgtac caaagaacat aatttttgtc attattaaaa acctcgcttt
     2341 taaaagattg aaaagtaaat gagtgaaatt aaagattatg tacattaaaa tcacgccaca
     2401 atttaattgt gaaaaatatc acaaatatat tataacacta aattttccaa aattcaaaag
     2461 tgtgttttat tgcagaaaac ttataacatg tgcacaagtt atagtgaatt gcaaacggat
     2521 tactttagtc tttttaaaac atgaagtata atttgtatag caataaatat aaaaattgga
     2581 ggctatgttc aatgagcaat atgaatcaaa caattatgga tgcatttcat ttcagacatg
     2641 cgactaagca attcgatcca caaaagaaag tttcgaaaga agattttgaa acaatattag
     2701 agtcaggtag attgtctcca agttctcttg ggttagaacc ttggaagttt gtcgtgattc
     2761 aagatcaagc gttacgtgat gaattaaaag cgcacagttg gggcgcagcg aagcaattag
     2821 atacagcgag ccattttgtg ctaatttttg cgcgtaaaaa tgtaacgtca agatcaccgt
     2881 atgtacaaca tatgttaaga gatattaaaa aatatgaggc acaaacgatt ccagctgttg
     2941 aacaaaaatt cgatgcattc caagcagatt tccatatttc tgataatgat caagccttgt
     3001 atgactggtc aagtaaacaa acgtatatcg cattaggcaa tatgatgacg acagccgcat
     3061 tgttaggtat tgattcatgt ccgatggaag gttttagtct ggatacagtg acagacattt
     3121 tagcaaataa aggtatctta gatactgagc aatttggttt atcagtgatg gtcgcatttg
     3181 gctacagaca acaagatcca ccgaaaaata aaacacgcca agcatatgaa gatgttattg
     3241 aatgggttgg accaaaagaa tagatagaat acagtatgtc taaatatata aaattaaaaa
     3301 gttagcaata aaaaagcctg cgattacata aatgaatcgc aggcttttgc gtgaaaaaat
     3361 tgtattaata aagtatggat gattattttt ctggaacaag gtcagtattt gaatgaactg
     3421 tgatgtcaaa cccttctggt gccgtaaatg tatgtgttga ggcgtcgggt tgataaatat
     3481 caacatgtgt taatccataa ctttgtgcat tgttttgtct tgcttgattg gattgccaag
     3541 tattagcagc aatatgatgg tgataatgat tcgttgacat aaatagcgca cgtggaaaat
     3601 cagatacatg ctggaatcct aattgttcaa tgtaacattg atatgctgcg tctaagtcat
     3661 gtgtttttaa atgtaagtgt ccaatcatgc cttttgctgg cattccttgc caaccttcat
     3721 cagtacgatg tgttaataag gtttggctat caacttctaa agtatccatt ttaactttgc
     3781 cattttgcca ttcccatgaa gatgaaggtc tatcgcgata gacttcaata ccattacctt
     3841 cggggtcgtt gaaatataaa gcttcactta ctaaatgatc accagcgccg attcccatat
     3901 ttttttgtgc cacgaaatat aagaagttag caagatcctc agtagttggt aataaaaatg
     3961 ctatatgaaa aagccctgct tcacgtgggg aagtctgacg gccgtcttct aataaatgta
     4021 acgttagagt atggccacca gtcccaacag ataatacggt tgtgttatcg tcagaacttt
     4081 taacgggtag tcctaaaatg tttttgtaaa atgttgtcat taagtctaag tctcttacgt
     4141 tcagttcaat gtttgtcact tgtgttgctg ttttatcgtg aaatgccatt atgcatcgcc
     4201 tcgttttcta tttttctata agttagtata aaaagtatac cagaaaagaa aatgaattga
     4261 cagcaaaaag tttgaaatgc aaaataacat gatgtttcgc aattttatat tgatacgaac
     4321 aaataaaaaa gcgatggtac tggagcacca tcgcgaggtt tatttaacca agatacacat
     4381 cttgatatga atcttctttt tctaacatat gtttggcaaa tgaacatgag gcaataattt
     4441 ttaaatgatt ttcgcgagca tgttcaacaa ctgctttaac tagttttttg ccaacacctt
     4501 gaccaccgag ttcatcagat acgcctgtat gatcaatatt aatttcatta ttatctacaa
     4561 aacggtatgt gatttcagct aaagcatgat tttcatcatc accaatatag aatttgttct
     4621 cgccttgttt aatttcaagg ttactcatac atatcaactc ctatcatgat tgattatagt
     4681 atttccctat tctattttaa cttaaacgaa gacaaaggtg catgacagtc atgtgacgac
     4741 attgccgctg tacaagtagt tatttttatt aagcgcagtt tgaaatgaag atgaaaacac
     4801 gtatcttgac attaaattca ttcagctata taatttatct cgaaatcgaa ataaattaaa
     4861 aagttggtga tgatatggat cgaacgaaac aatcgctcaa tgtctttgtc ggaatgaata
     4921 gggcgttaga cacattagag caaattacaa aagaagacgt aaagcgatat ggcttaaata
     4981 ttactgaatt tgcagtgcta gagttgcttt ataataaagg tccgcaacca attcaacgta
     5041 ttagagaccg cgtattaatt gcaagtagca gcatttcata tgttgtaagt caattagagg
     5101 acaaaggttg gattacacgt gaaaaggata aagatgataa acgtgtatat atggcttgtt
     5161 taactgaaaa aggtcaaagt caaatggcag atattttccc taagcatgct gagacattaa
     5221 caaaagcgtt tgatgtgtta acaaaggatg aattaacaat cttacaacaa gcgtttaaga
     5281 aactaagtgc acaatctaca gaagtgtaag gcgtgcacta aaaatttaca ttaaaatatc
     5341 tcggtttcga gataagtaca ctaaaaatat aaagagggta tataaaatga caaataatca
     5401 tgaattacta ggtattcacc atgtcacagc aatgacagat gatgcagaac gtaattataa
     5461 attttttaca gaagtattag gaatgcgttt agttaaaaag acagtcaatc aagatgatat
     5521 ttatacgtat catacatttt ttgcagatga cgtaggttcg gcaggtacag acatgacgtt
     5581 ctttgatttt ccaaatatta caaaagggca ggcaggaaca aattccatta caagaccgtc
     5641 ttttagagtg cctaacgatg acgcattaat atattatgaa cagcgctttg atgaatttgg
     5701 tgttaaacac gaaggtattc aagaattatt tggtaaaaaa gtgttgccat ttgaagaagt
     5761 cgatggccaa gtgtatcaat taatttcaga tgagttaaat gaaggggtag cacctggtgt
     5821 accttggaag aatggaccgg tgccagtaga taaagcgatt tatggattag gacccattga
     5881 aattaaagta agttattttg atgactttaa aaatatttta gagactgttt acggtatgac
     5941 aactattgcg catgaagata atgtcgcatt acttgaagtt ggcgaaggag gcaatggtgg
     6001 ccaggtaatc ttaataaaag atgataaagg gccagcagca cgtcaaggtt atggtgaggt
     6061 acatcatgtg tcatttcgtg tgaaagatca tgctgcaata gaagcgtggg caacgaaata
     6121 taaagaggta ggtattaaca actcaggcat cgttaatcgt ttctattttg aagcattata
     6181 tgcacgtgtg gggcatattt taatagaaat ttcaacagat ggaccaggat ttatggaaga
     6241 tgaaccttat gaaacattag gcgaagggtt atccttacca ccatttttag aaaataaaag
     6301 agaatatata gaatcggaag ttagaccttt taatacgaaa cgtcaacatg attaattgga
     6361 atgagaggga tttgtgatgg aacatatatt tagagaagga caaaaaggtg cgccgacgct
     6421 aatattattg catggtacag gtggtgatga gttcgattta ttaccattag gtgaagcatt
     6481 gaatgaaaat tatcacttgt taagtattag aggacaagtt tcagaaaatg gaatgaaccg
     6541 ttatttcaaa cgtcttggag aaggtgttta tgatgaagaa gatttggcat ttcgtggaca
     6601 agaattgtta acgttcatta aagaagctgc tgaacgttat gattttgata ttgaaaaggc
     6661 tgtacttgtt ggattttcaa atggatcaaa tatagcgatt aacttaatgt tgcgttcaga
     6721 agcaccattt aaaaaagcat tgttatatgc accgttatat ccaattgaaa taacgtcaac
     6781 aaagaattta tcagatgtca gtgtgttgct ttctatgggg aaacatgatc caattgtgcc
     6841 attagctgca agtgaacaag tcattaactt gtttaagaca cgtggggcac aagtcgaaga
     6901 agtttgggtg aatggtcatg aaattacaga aactggatta acggctggtc aacaaatact
     6961 tgagaaataa cagttctatt aagaagcgga cagatggaaa agatttttac ttttcatctg
     7021 cccgcttttt tgattttgaa gtgttgttct aaattttaca atagtataga tattttaatc
     7081 gatatgagat ttgccggtaa tacgcttaat taaaccttta tagagtacag gtatgagtaa
     7141 aatgaaaccg aacaatccca taatagggaa tacttttcca attaatgaaa tgaaaccgat
     7201 aaatgtacta atataagtga tgacagccat tgtaataata atgatgaagt aacgtctgct
     7261 aaatggaacg ctgaaacgtg acgcaaatgc atacattaat ccaacaacag tattgtagat
     7321 gacaagtatc ataatgacag acataataat accaattgac ggagacattt gtgtcgctaa
     7381 ttttaatgta ggtaaatcga cgtgtttaat tttatcgaat tgagaaatta aacctaggtt
     7441 aatcatcatt agtaaaaatg taatgattaa accgccaatc aagcccccgt ataacgttga
     7501 gtcacgatac ttaactttac tacccatcac tgataagaag ctgaaggcag cagcaatttg
     7561 caagcttgca tagttaatcg catcaaacca ccatccaggt gataatgatt tctgcttatg
     7621 aatctgagca tcattattag cggcagtaaa atcaagatga ctcgttgtga aatagtagac
     7681 cgcaatcata atgacaatcg caattaaaaa tggggtaact ccaccaagca cagcaattaa
     7741 acgatcgaat tttagaaaca gtgttgctaa aataaaggcg actaatatga gtgcgctcag
     7801 ccaatacggt aagttgaaac tttgatgaat ggttgacgcg ccacctgcag tcataataat
     7861 agctaaagac aacataaaca ttgtcaaaat aatatcaaaa cctcttgcaa tagaggggta
     7921 taagaaatag ttaattgaat cagaatgatt tctagacttt agatgatgac ctgtatgcat
     7981 gacaatcatt ccacctaaag taatcaatag tcctgtcaca ataatgcctg aaatgctata
     8041 cgcgccatga cttgtgaaaa actggaaaat ttcttgacca gtagcaaagc cggcaccaac
     8101 gacaacacca acaaaggcaa atgccacaat aatggactct tttaagatgc gcatgattta
     8161 aaaatgtccc ttcgtaattt taagtaatat agaaaatgta acatacatgt taatgaaaaa
     8221 tatagtacta atatagtatt ttgttaaatt ggagtagaag caagggcgtc ggtcatttta
     8281 ttaatttatt agttaatttt gcattttttt gctgtgaagt tgttataata caggtaacaa
     8341 ggaactagca tagatacacc aatcccctca ctactcgcaa tagtgagggg atttttttcg
     8401 gtgtagctag gtcgcctatt taccatcgcg tttgcgtagc caatgcgtaa acactgcacc
     8461 actatataag tgcacgatac atgcatcaaa tgtcgtcttt agtctaagta acgatcatgc
     8521 attaacattt tcaaaatatc tatttgagcc tgaagctctt tacctttatt tgtattgcgg
     8581 atttttttac gttgtaattc tttatcaaca atacgtttaa ttgacagttc gtcgatacct
     8641 tcggaaagaa ttttttcttt tgcgttaaat tgttgatgtg caacgagttg cattccgaat
     8701 gaattataca acaatgtata gcctgcaatg ccagttgttg actgataagc ttttgaaaag
     8761 ccaccatcaa tgacaagcat ctttccatca gccttgatag gatcttcccc attaatttct
     8821 ttcactggtg tgtgaccatt aataatgcgt ccttcatctg gatttaatcc gaaatcactg
     8881 agcattttac gcaccatatt cacatcttca cgaagatgat agtacggatt cttttcttct
     8941 ttatgagaag ctttatctgc aataaagtat cgttcaaacg tagtcatcgc acgtttacca
     9001 aatagtgaag agtatttccc agtccataaa taccaaacta aatcagtgga taagtcatca
     9061 gtattttctt tttcatcaaa tgatttacgg acatgatatt caaacacatc taataactct
     9121 tggccactgt atgtctgacc atcaatttcg aatgattcca tctcaccatt ttcatcaact
     9181 ggaatacaac catgaatgag taaattgcca ttacaaggta agtaaagtga gcctttacgc
     9241 atcaagaaag acatatgacg acgtaatttt tcagattgtt ggaatgacaa taatagttta
     9301 ttcatgactt cttcttcttc aggtagtaat tctgctgggt tatcacggtt aacagtttgg
     9361 aagcatgtat cttttaatgg atatgttttt ccgtatactg ttatttcatt agtatcataa
     9421 ttaacttttt caagtacaag tcgttcgtcc atttcaaagt ttggacggcg cttaataacc
     9481 ggtatttcca gtttgaattg aatcatcgca atagcttgat gaattttagt aatctgactt
     9541 tcttcgcgtt gtgttaaacg ttcatgtttg tcaggtcttt ttttaggttt aaaagcaggg
     9601 ttatctgcat catagtattt ttcggctaat gttagcaaag gtcttaaatt aataccgtaa
     9661 gcatcttcaa cgatatctag attgtcataa cgtgcacaaa tacgtagtaa attagctaaa
     9721 catactttag accctgcata agcaccaacc catagcacat catgattacc ccattgaata
     9781 tccagagaat gataattaat cagcgtgtcc atgattttat ctggttgcgg gccacgatca
     9841 taaatatcac caacaacgtg taaatgatcg acgactaagc gttgtacaga atacgcaagc
     9901 cctataatta aatcatccgc ctgcttaagc tcaatcactt gatttacaag tgtttcgtag
     9961 taagattttt tatttttgaa ttcgttgttc ttatataata actcttctac gatgaaaacg
    10021 tattgctccg gcaaggcacg tcgaagtttt gaacgcgtat attttgaaga acaatattta
    10081 attagctgaa tgaggtgttc aatcgtcgta atataccaaa catttaattg tccataattt
    10141 tgaaaatcac atttaataag ttgcaatttg tcttctggat agtaaacaag ggcagtcaaa
    10201 tcgttaagtt ctttcgttga taacttgtct ttgaaaatat cattaatttt cgctcgcacg
    10261 ttcccagaac cgttgcgtaa tacgtgttgg aaagcttcat attcaccatg taaatcgcta
    10321 acgaaatgct ccgtaccttt aggtaattct aaaatagatt ctaaattaat aatttcagtc
    10381 gcaagctttt ctggagtatc gaaatgttga gataataaat ccaaatattt cttttttaat
    10441 tctctttcag taatttgagt cattgtgtgt cactcctgta gtatatttca atcattggtt
    10501 tgtaaaatta atacgcttac aatgattatc aagaaaacgt taacattttt caataagtat
    10561 gcttgaattg ataaaaatag agggcagaat tttggtatta ggagccgttg cagtttagtt
    10621 aagtaaaagt ggagaagtga tatatctatt ttaaaaatga gaggagggat ttgttatatt
    10681 cactagtttc ctaaaaaatt acgatatgta aaatgtggaa ttgccaaaat ttggatgaaa
    10741 aaaatccccg caccattatt tgggtgaagg gatttgaaaa tgattttgaa ttacgatgga
    10801 atatgtgtgt atttagacat ctaatacttt aagttactaa atatttttct tcttgaaaat
    10861 aaatactaca agtattaaga aaatagaaat ataagcaata ttaccaataa acaattcatg
    10921 aagttctaat ttcgtcgact tactaaagcc tttttcaact gtttgaagca taatattcat
    10981 catattaata ggattccact ttagccagtc tattttttct aaaagtgccg tttgaataac
    11041 tgctaaaata gaacttgcaa aataaaaaac aatacctaca gcaatggcta cacccgttga
    11101 attagttgca gatgataata gcaacgttaa gcttaaaact aaccaaacgc caacaaatgt
    11161 acctaaacta actaataata attgattcaa taaagataat tgattaccgc tactttcaaa
    11221 tatatttaaa tcattaaaga ataaagaccc aataacaatt gaagcaataa ttgtaataac
    11281 aaaataaatt aaagaaataa taaataatgt gatgattttg ctaacaatca tagttgttct
    11341 tgaatattca cgatagagta aatttttaat cgtaccgtaa tgaaattcca ttgaaatgat
    11401 tgtacttgct tgaataatta ataaaaatgc aaaccatgaa aaaccattat aagcagatgt
    11461 gaaagattcc tgtggcgtaa aaatttcatt gtattttgta gcgatataac cttgagcaac
    11521 cattagtaga ataaagacaa taggtgcgat aaaagttgat ttctttttaa ataatttgaa
    11581 acattcttgt ttaattaaag ttcccatttt atgcttgatc ctccctttct atgatttcta
    11641 gtaatacatt ttctaaatca ttttctttaa attgtttaag ttcttcttcc gatgtttctg
    11701 taacaatttt acctttgtta ataataagga tagaatttgt gattttaact aattcactta
    11761 aaatatgact cgaaattaag aaagtgacac cttcttgcgc tttttggaca atcaattcac
    11821 gtacatctcg cacagctttt ggatctaagc cattcattgg ttcatctaag ataatgaatt
    11881 gaggtttatt taaaaatgct atagcaattc ctaatttttg tttcatacca agagaatacg
    11941 ttttagcttt tttatgaatg tattcatcca tatgaagttg tgagacaatt ttatcgatat
    12001 cttgagtatt ttttgattcg ttcaataact tcaagttttc atatccagac ataaaaggat
    12061 atattcctgg attttcaatc aatgcaccga tattgctttt gctgtccttg ctgttaataa
    12121 cattaaaatt cccactttgg aaactagagt aacctaatat aactttcatt aaagttgttt
    12181 tacctgcacc attcgctcca acaagaccaa ctatttgtcc gcgtttcagc ttaaatgata
    12241 catcttcgag aatcgttttg ttgcctatct tctttgttaa atgttctatt gttaaaacat
    12301 ccatatcact atctccttta ctattaaata atctgaatct taagatttta tatataaaat
    12361 taattttatc atatagcaag tgttataaaa atatgtattt aaaaaactta aataatataa
    12421 aatataaata ttaatatgag tattttttca gcatgcaaat ggcattgcta catttattaa
    12481 aatgcgcaat ttattatgag tgactttttt gaatagaagg catattaaag tgacgaaaat
    12541 agtttgtgtg gaagacaggg taaattctgc ttatttaaaa tgatagtttg tattatagaa
    12601 ggatttgtga gatgcgtttg cggaattaat ggtgcataac taagtaaata tatgttaatt
    12661 acaacaatat ttgacaaaaa tgggagtgac aacaataata tagttaaccg gttaactaat
    12721 gaagaggagg aacaatagtg gatatgaaga gagaagttat tttaaagtta caacaattta
    12781 ttatagaaag agaaaatgcc gataaaagaa gacagtataa aaaggaaaat gaggaactgg
    12841 atttatctct aacacaattt catatcatag agttgattga taataatgat aaagtgaata
    12901 ataaattttt gtctgaaatg ttaaatgtat caaaaccagc aattactaaa tcgataaaaa
    12961 aacttttagc gaaaggctta gtagttgaat cacacaatga atttaacaaa agagaagtta
    13021 attattcatt aacacaagaa ggaaaaaaat tatcttatat acatgatgaa ttacatgaaa
    13081 aagcggtaaa aaaatatgaa gaagtactta aagtctttga taatgatgaa atggcagtta
    13141 ttgtagagtt tttaaaccgt agtatagaag aattaaaaaa agaagaagat ggtttaaatg
    13201 actaaaagta atatcagatt atatattcta ggatttttag catttttcgc atcattgatt
    13261 caaaacatat acacgcctat tattccgaga ctatatgacg attttcaagt accactattt
    13321 tggataaatg cgacagttgg cggttttata tttatcgttg cagttatgca aattgtactt
    13381 ggtagaagta tagattctcg tgattctata aaagtacttt taacgggatt gggaatagta
    13441 atcatatcta gctttatttg tgcagtgaca cataacttta tattatttgc tatatcaagg
    13501 ttatttcaag caattggttg cggaattatt cctctcgtga ctttaacttt attagccaaa
    13561 ctaagtacag ataatggtag agctcaagct atggcgaact atcaaatatt tttatcatgt
    13621 gcgccaactt tggcaccaat cttagggagt actttaggcg caagatggga ttatattggt
    13681 atttttagtt ttttgctcgt tatatctatc gtattatttc tgattatctt ttttattgat
    13741 attccaaatg ttgaaaaagg gattgtaaaa ttaactgaaa aaattgaaga aaaatatttg
    13801 accgataaag tgtttattac tttagtgaca ttgggatttt taatattttt tacgtacttt
    13861 tcaatattag tttatttacc aacattatta aataatacct atgatatagg cgttggaata
    13921 tcaggagttt tgtttttacc tattactgtt agtgttatat taggaagttt attttataag
    13981 cgattttcaa aaaaatataa tgaaataatg atacttcgtg ttactataac tggatttgct
    14041 atgtttacgt tattgtttgg ttggttaaac gaatcaaatg taattatatt atcagtaatt
    14101 atatttatat taggtacttg tgttggtatt gtgccagctt tgctggctac tataatttct
    14161 aaaagatttg aacatataaa aggaaaagtg ctaggcgtat ttaattttgt cagatatatt
    14221 ggaatgactg taggtgcatt attaattggt atcatttctc agccgttggt agccttttac
    14281 ttcacaacta taactatcat gttaataata atatttcttt atataaaaat aggtgacttt
    14341 cagctaaagt atgccaaata aacttaaaaa agcagtgaat catttcaata gatggaatga
    14401 ttttactgct ttttgtttat agaaatttaa aattcatgcc tttatgctta caacaatatt
    14461 attttactct cttacgtttg ccaatgtaac gtatagcaaa gtaaatgact gcgataatga
    14521 tgacaacata cataatacgt gaatatgtat gaagaccagt catcaacata ccaaagctat
    14581 cgctcaacgt gcgtcctagt aaaattaaac cgaagttcca aattgttgta cctattaatg
    14641 aaataacggt aaatgtcaca acattcatgc ggtttacacc ggctggaatg gtaatcaata
    14701 cacgtaatac aggtataaaa cgacagataa atacagccca tacgccatac tttttaaacc
    14761 aatcatttgc tcgctttaaa tccttacttt tcaatttaat ccacttaccg tgtcgatcaa
    14821 taaagcgata tagacgttct tctgaaatca aacgacagat ataatataaa attaacagtc
    14881 ctataaacga tgcgatggtt gcaataataa ataaggttaa aattgataaa tgtgatttaa
    14941 cagacataag accagcaaat gtcagaataa tttccgatgg tacgataggt aaaatatttt
    15001 ctaataaaat taatataaaa atggctgcat acccaaaacg actaataaat tcagtgataa
    15061 tttgttccat tattaaaaat aggctccttt aattttcagc aattgcagac aaatagaaac
    15121 tggataaatt caaatgaata atgaatgatg tcgtatgcga tgttgaaaca agcatttgtc
    15181 ttaaaatgta ttataagcaa taaatcaaaa aattgcttgc tataagtacg aaaagtcaac
    15241 aaatgatcat ctaaataaca ggcgctgcac aatgaatgag caatgtagtt ttatgcaatt
    15301 taaaatgaat gtcaaatatg taagaaagcc tgcaatatac gttttaaaac actagtattg
    15361 cagtattttt aattaacaaa tgataggcta ctatgtaact aaaagttcct ttatatcgtt
    15421 ttaatagcaa aagttaagcg gaatttaata aaatttttat agattctatt gtaaacgctt
    15481 tccatgtctg ataatattat aaaaaataac tttggtacaa ttttgtgaca aagtaggggt
    15541 ggacatgcca gatgtccgaa aattttaacg tttcagggta agttttaaaa aattaattag
    15601 ggaagtagga atagttatga aggaaaaacg aactaacgta aggtggatgt tcgcgcttgc
    15661 gttcttcttt attggggtta ttgcgtatat ggatagagca aacatttcat atatcgctaa
    15721 gcaaatgatg gatgatttag ggatgacaaa accacaattt ggtttattgg catcattctt
    15781 ctctctaggt tatgcattaa tgcaagtacc atcggggatg ttggctgaaa aattcggtcc
    15841 acgtaagatg attactattg cattagtttg gtggagtgca tttacaatct taacgggtat
    15901 gattaagaac cacggtttaa tttatttagt gagattctta tttggtgtgg gtgaggcgcc
    15961 aatgtaccct tctaatgctg tgtttaactc attttggttc tctaaaaatg aaaaaggtag
    16021 agcatcaagt gcattattag caggatcata tttcggacct gtattagcac caatagttac
    16081 aattgctatt gttaacgcat ttaactggca agcagtattt tacatttttg gtgcagtagg
    16141 tattttaatg gctgtattat gggcgattat tgccaaagac ttacctgagc aacatagaat
    16201 ggttaatgaa gcggagaaac gtttcattat ggaaaatcgt gatatcgtag ctactgaaaa
    16261 gtcatcacca ccatggaatg atttctttaa acgttttagc ttctatgcaa ttgcaattca
    16321 atactttgtt gtacaattta tcattacatt attcttaatt tggttaccga cgtatttaac
    16381 agaagtattc cacgttaact ttaaagaaat gagcattagt tcattacctt ggttattaat
    16441 gttcttctta atcttatcag caggtgcaat ttctgaccgt gtattaggat taggacgttc
    16501 aaaattcgta gctagaggtg tcattgcaat cgcaggattt attgtatttg cagtttcaat
    16561 tatctttgct gtacgtacag gaaatttata tgtaagtatt ttctggttat cactaggtct
    16621 tggtggtatc ggtatttcaa tgggtatgag ttgggctgca gcaactgatt taggacgtaa
    16681 cttctctggt acagtatcag gatggatgaa cttatggggt aatataggtg cattaatcag
    16741 tccgctatta gcaggtctat tcgtagaaca tttaggttgg acaatgacat tccaattgtt
    16801 aatcgttcca gcagtaatcg ctgtgattat gtggttctat gtgaaaccag atcaaccttt
    16861 aattgttagt gatgataaag caatagaaaa ataatttaaa catgcagtaa gctttcacat
    16921 tgttgaggct tattgctttt tttacgttaa aattgaaaaa ttttaaaaca gatatggttt
    16981 aagatgaatg ggaagttatt aaaatgatat atgtagaaga aataaagttt taaaacctta
    17041 gtcaggtaac gcttgtaaaa gtacatataa attttaggag gttataaata tgaagggaaa
    17101 cttagaaaaa tatattagaa gtttgcctct tataggtcta attataagta tttttttaat
    17161 tattttatac ttcttaattt atcgagtaga aggaaatttt tgtgtaatca ttttgtactg
    17221 cttattgcca ttatttgtaa atacgtcact atatatatta tacataatca tctttcgata
    17281 ttttaagaaa tgaactgaca tcgaatgtca ttttaaacaa caaatata
//
LOCUS       DAPIDM010000042        17296 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000042 DAPIDM010000000
VERSION     DAPIDM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.042"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            244..1368
                     /locus_tag="RJE42_001258"
     CDS             244..1368
                     /locus_tag="RJE42_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDV5876687.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLKGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVDV"
     gene            complement(1563..2789)
                     /gene="pepT"
                     /locus_tag="RJE42_001259"
     CDS             complement(1563..2789)
                     /gene="pepT"
                     /locus_tag="RJE42_001259"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV5876688.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGDTLMVTDGTSLLGADDKAGIVEIMEAICYLQKHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENN"
     gene            complement(2803..3297)
                     /locus_tag="RJE42_001260"
     CDS             complement(2803..3297)
                     /locus_tag="RJE42_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDV5876689.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(3315..4076)
                     /locus_tag="RJE42_001261"
     CDS             complement(3315..4076)
                     /locus_tag="RJE42_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV5876690.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRIQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(4272..5342)
                     /locus_tag="RJE42_001262"
     CDS             complement(4272..5342)
                     /locus_tag="RJE42_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV5876691.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIR
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            5660..6715
                     /locus_tag="RJE42_001263"
     CDS             5660..6715
                     /locus_tag="RJE42_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDV5876692.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKED"
     gene            complement(6879..7520)
                     /locus_tag="RJE42_001264"
     CDS             complement(6879..7520)
                     /locus_tag="RJE42_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDV5876693.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            7664..8530
                     /gene="fakB1"
                     /locus_tag="RJE42_001265"
     CDS             7664..8530
                     /gene="fakB1"
                     /locus_tag="RJE42_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDV5876694.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHLEDGQALYKKLQEDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            8894..9976
                     /locus_tag="RJE42_001266"
     CDS             8894..9976
                     /locus_tag="RJE42_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV5876695.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     IEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGQF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     CMNMIQAKKEIQKMNKLALKRGWIDE"
     gene            9969..10643
                     /locus_tag="RJE42_001267"
     CDS             9969..10643
                     /locus_tag="RJE42_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDV5876696.1"
                     /translation="MNNCLSCGAELYEKITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKYLNQNEAYCLDCKFLSAHFNLMEQLYCQFQYDGIMKEMIHQYKFSKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGILFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDMELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            10704..11276
                     /gene="raiA"
                     /locus_tag="RJE42_001268"
     CDS             10704..11276
                     /gene="raiA"
                     /locus_tag="RJE42_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV5876697.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            11690..14221
                     /gene="secA"
                     /locus_tag="RJE42_001269"
     CDS             11690..14221
                     /gene="secA"
                     /locus_tag="RJE42_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV5876698.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTDFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            14263..14382
                     /locus_tag="RJE42_001270"
     CDS             14263..14382
                     /locus_tag="RJE42_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876699.1"
                     /translation="MLWEESFYFTSIKYFKKCEEVVKRCYVLSFKKIGLGICR"
     gene            14537..15647
                     /gene="prfB"
                     /locus_tag="RJE42_001271"
     CDS             join(14537..14608,14610..15647)
                     /gene="prfB"
                     /locus_tag="RJE42_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV5876700.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            16253..17092
                     /locus_tag="RJE42_001272"
     CDS             16253..17092
                     /locus_tag="RJE42_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5876701.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLMLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKTVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQTVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
BASE COUNT         6167 a         2518 c         3088 g         5523 t
ORIGIN      
        1 ttattgaaaa tattttggaa aacgacgaat ccaataagaa agtttaaaca tgatttgtaa
       61 gttagtttaa tagcaaatat atgctaaacc agaagaagca tactgttatt tactggaata
      121 attaataatc atgtcatgtt aaatgttcgc atataatcac gagataaaat ctgaaattta
      181 agattaatct tttatgaata aaaaacgtat cacaacaaat aataaagtaa ggtggtcaag
      241 gttatgaaag tattagtagc tatggatgag tttcatggaa ttatttcgag ttatcaagct
      301 aatagatatg ttgaagaggc agttgcaagc caaattgaaa ctgcagatgt agttcaagta
      361 ccattgttta atggaagaca tgaattatta gattctgtat ttttatggca atctgggcaa
      421 aagtatcgta taccagtaca tgatgcagat atgaatgaag ttgaaggtgt ttacggacaa
      481 actgatacag ggatgaccgt tattgagggg aatttatttt taaaaggtaa aaaaccaatt
      541 gttgaacgaa caagttatgg tttaggagaa atgattaaac atgcattaga taacgacgca
      601 aaacatgttg taatttcact aggtgggatt gatagttttg atgctggtgc aggtatgtta
      661 caagcattag gtgctcaatt ctatgatgac gaagggcgtg tcgtagatat gagacaaggt
      721 gctggcgtaa ttaaatatat tcgtcgtatg gatatgtcga acttacaccc taaaatggaa
      781 acagcaagaa ttcaagtaat gtcggatttt tcaagtcgat tatatggtaa gcaaagtgaa
      841 atcatgcaaa cttatgatgc gcatcagttg aatcataatc aagcagcaga aatcgataat
      901 ttaatttggt attttagtga gttatttaaa agtgaattga aaattgcaat tggtccagtt
      961 gaacgtggtg gtgctggtgg tggaattgca gcagtcttga aaggactgta tcaagctgaa
     1021 atattaacca gtcatgcatt agtagaccaa ctaacgcatt tagaaaattt agttgaacaa
     1081 gcggatttaa ttatttttgg agaaggatta aatgaaaatg atcagttgct agaaacgaca
     1141 acattgcgta ttgcagaact ttgtcataaa catcaaaagg ttgccattgc aatttgtgca
     1201 actgctgaaa agtttgattt atttgaatca caaggggtta cagcaatgtt taatacattt
     1261 atcgatatgc cggaaagtta taccgacttt aaaatggggt tacaaattag gcattatact
     1321 gttcagtctt taaaactgtt gaaaacacat tttaatgttg atgtttagta aagaaggact
     1381 aaattggtga tgttgtcatg atggttaata acatttatga tggttagcaa aacgaattag
     1441 aagatcgaaa gtatacttaa aataaaataa catgaaaaaa tcacgctatc attgcactga
     1501 atgttagcgt gattttttat attaattaag cctgagttga actagtatat aatcgttggt
     1561 ttttagttat tttcagcgat atcttctaca attccaatga ttacttgcac tgctttttcc
     1621 atgacatcaa tggatgcata ttcatatggg ccatggaagt taccgcaacc tgtaaagatg
     1681 tttggagttg gtaaccccat aaatgataat tgtgaaccat ctgtaccacc acgaataggt
     1741 tcagtgtttg ctggaatatc taatttagca aagacacgtt taggtatatc aataatatga
     1801 ggcaatggta atattttttc tgccatattg aaatattgat ccgatatatc aactttaact
     1861 ggataatttt caaaatgggc attgatatcg tcacgtattt ctaaaatacg tttcttacgc
     1921 aattcgaatt gttttttatc atgatcacga ataatgtatt gcaaagttgc tttttcaaca
     1981 gttccttcaa agttcattaa gtgataaaag ccttcatatc cttctgttcg ctccggaact
     2041 tcactatcag gtagcaaact atcgaattgt tcacctaaac gtattgcgtt taccattgca
     2101 tttttagctg aaccaggatg aacattcaca ccgtggcatg taataaccgc ttcagcagcg
     2161 ttaaagcttt catattgtaa ttctccatat tgactaccat ccatagtata agcaaaatcg
     2221 gcattgaagc ggtcaacatc aaatttatgt ggaccacgac cgatttcttc gtctggtgta
     2281 aatccaatgc gaatggtacc atgtttaatt tctggatgtt tttgtaaata acaaatagct
     2341 tccataattt ccacaatacc cgctttatcg tctgcaccta gtaacgatgt accatcagtt
     2401 accattaatg tatcaccaac taaactgtta agttctggaa atactttagg atctaagaca
     2461 cgtttagtat tgcctagttt atatggcttg ccatcatagt tttcaataat ttgcggttta
     2521 acatttgaag cattgaaatc aggtgatgta tcaacatgag ccaaaaaacc aacagttggg
     2581 acgtcaacat cgatgttact ttctaatgta gcaaataagt agccattttc atctaaatca
     2641 gttggcaatc ctaattgttg taattctttt tctaataaat gtaacaaatc ccattgcttt
     2701 tcagttgaag gtgttgttgt agattttgga tcagattgcg tatcaattgt cgtatatctt
     2761 gttaatctat ctatcaattg gttcttcatt atattcgacc ccttaaactc tattattcat
     2821 gttgtaagat tttttatatg tcttaccttt gattttacca tacagttgtt tgatacgtgt
     2881 gtataggtaa tatagaattt cagaaactaa tataccgaaa gcaatcgcac ctgaaatcag
     2941 tgtaacttct aaaaatgtat ttacagcact tgtataatca ttcgatacta aaaaacgagt
     3001 cgcttgataa gctgcaccac caggtactaa tggtataatg ccaggcacta tgaatataat
     3061 taccggtcgt ttatatctgc gactcatagt atgactcatt aagcctaaaa ttaagcttcc
     3121 caaaaatgaa gcgccaactt ttccaaactc taaatctacc gttaattggt aaatcgtcca
     3181 tgcaatggca cctacaaatc cacatgctac taagagacgt ttgggtgcat tgaaaatgat
     3241 agagaaaagt actgttgata taaagctgat tgtaaaatga aataaataaa ataacatgct
     3301 ttaacagtcc ttccttaaat gattaataaa acgattgcga caccagcacc gattgcgaat
     3361 gctgttaatg cagcttcaac accacgagac atacctgcaa gtaattcacc cgctaataaa
     3421 tctcgaatgg cattggtaat taatatacca gggacaagtg gcatgacact ggctatagta
     3481 atgatatctt gattggttgc aatacctaat ttagtaaatg tggctgcaat ggatatgacc
     3541 acagcggctg caacaaactc tgagaaaaat ttaatttgta tatagcgttg cacaaagctg
     3601 aatgttaaaa atgcggatcc accagcaatg actgcaatcc aacaatctga tgcgacacca
     3661 ccaaacatga acaggaagaa gccacatgca atggcagcgg caaagaaatt cgttaaaaaa
     3721 gaatattgta atgatgcatg ctgtaaatga ataaattcag atttagcttc atcaattgtg
     3781 agttctttat ttgatatttt acgtgaaaga ctattcgtta aagcgatttt ctctaaatct
     3841 gttgtacgct cttgtatacg aattaatctt gtacttgttc gatcgtttaa tgaaaaaata
     3901 attgcagttg aactgacaaa actatatgta ttatgaagac cataactatg tgcgatacga
     3961 ttcattgtat cttcaactcg atatgtttca gcacctgatt caagtaaaat tctacctgca
     4021 attaatacaa catcaatcac tttgttttca tctataattg tgattgaatc tggcatatca
     4081 attcacctcc aatgatatgt gttatttatt tgaacaattg aagtttacaa cttgttgttg
     4141 caactttcaa tagtgagact ttgtgttagt atgatgaact tgtatggttc aaatttaaat
     4201 aagaaaaact gttaatcttt gctattatac tatgatttaa taatagcaaa ggattaacag
     4261 ttttgtcgtt gttatagatt gatgataggg ttaaacatta ctttatttcg cccttgattt
     4321 ttggctacat gcaccatatc gtctgcatct ttaaacactt tacgctgtga ttttggatcg
     4381 tcatctgtta aataaccaac accgatagac actgacaatt taataacttc tttgtttggt
     4441 aaatggaatg atgatttttc aacacccgaa cgaatatttt cagctaattt aacactttga
     4501 tcaagtgaat agttgcgaat gacaactgag aactcttcgc caccatttct aaaaatttta
     4561 aattgattcg gcacatagtt tttaagtaat tgagacattt gttttaatac agcatcacct
     4621 gatttgtgtg agtaggtatc attgacatct ttaaatccat cgatatcgat taataataat
     4681 gcgatacttt gatgttcttt ttcagctttt cgtgaaattt catttaaatg tctatcaaat
     4741 tcttttacat tacctaagcc tgttaagtaa tcatatttat cttcgttttc ataacgattt
     4801 acgagtgaga agaaatgcca aatatcgaca aatgttatcg ctgaagctaa agtgataatt
     4861 aatgaaattg gtattaaaat gataacttcc gatagtgtgt aaataggact cactaatgcg
     4921 acaccaaata aaatgattat tgtaacaaca ttaagtatta ataatgatag cacatcattt
     4981 tgttttaaaa atggtccaat agcacttgtt actgcggcaa taacaatcaa cgtaacaccg
     5041 tacataatcg agttgttaaa tactacaatt tcaacaattg ctacaattac tgtggcagat
     5101 aatgtataga ccatatttgt aaatctacct aaaaacaata aaggaacgaa tgttaagtga
     5161 attaaataat cttcacgata agggataggg tagacagata ataataatga tacgattgtc
     5221 attaatacag tgacatatgc cttagaaaaa accatacgtt tgttttctga atactgtaag
     5281 cgatggaata aatagattcc agcgactata acagatatat tgtatataaa tgcttcgaac
     5341 atgtctgaat cgactccttt aattgaccac tagctattgt aagtgaaaac ttacaatttg
     5401 tcattagttt acatataaaa ttaatgtatg atatagactt tgatgttaaa atgttgcctt
     5461 aaatgatatg atgaaaaaat gaataatagg cgatatataa gaaatgaatc gtatagttgt
     5521 aaatatgata tcattgattg agcgaattaa tttataataa agctataaga tatacgtaga
     5581 aaatagatat atcattctat aaagacaata ttaaataaat ataacgttaa aaacaattaa
     5641 tatcgatgaa ggtgaataaa tggttacatt attactagtt gcagtaacaa tgattgtcag
     5701 tttgacgata acaccaattg ttattgcaat atcgaaaaga ttaaatttag ttgataaacc
     5761 aaattttaga aaagtacaca ctaaacctat ttcagttatg ggtggtacgg tgattctctt
     5821 ttcattttta ataggtattt ggattggtca tcctattgaa acagaaatca aaccacttat
     5881 tattggtgcg attattatgt acgtacttgg gcttgtagat gatatctacg atttgaaacc
     5941 gtatataaaa ttggctggtc aaattgctgc tgccttagta gttgcttttt atggtgtgac
     6001 tattgatttt atttcgttgc caatgggtac aactattcat tttggatttc ttagtattcc
     6061 aattactgtg atttggattg ttgctattac aaatgcaatt aacttaattg atggactcga
     6121 tggtttggca tcgggtgttt ctgcaatcgg actcattaca ataggattca ttgcaatttt
     6181 acaagctaat attttcataa cgatgatttg ttgtgtttta ttaggctctt taattgggtt
     6241 tttattttac aatttccatc ctgccaaaat atttttaggt gatagtgggg ctttaatgat
     6301 tggatttatc atcggattcc tttctttact cggattcaaa aatattacaa ttattgcatt
     6361 gttcttccca attgttatcc tagcagttcc attcattgat actttgttcg caatgattcg
     6421 acgtgtgaaa aaagggcagc atataatgca agctgataaa tcgcatttgc atcataaact
     6481 attagcttta ggctacacac atagacaaac agtattatta atctattcaa tctctatttt
     6541 atttagtctt tcgagcatta tcttgtatgt atcgccacca ttaggtgttg tattaatgtt
     6601 tgtattaatc atatttagca ttgaattaat tgttgaattt acaggattaa tagataacaa
     6661 ctaccgacca atattaaatt taattagtcg taagtcatct cataaagaag attagatttt
     6721 aaaacggtaa gtaataaaat aaaaaatgat gtctgtactt ttatgttgtt ataaagaagg
     6781 catactcaca aaaaacacta gcatggaacg tgctttccaa attgcaaagt aacaatacgc
     6841 aattagagtt agccatccta agctagtgct ttttattttt attcagttgg tatatcgaaa
     6901 ggtaactgct ttggagtttc ttcagtcaaa tcgaaatttc cagcagtcat ttgatttaaa
     6961 aagttaataa acgcttcata gtcactttta acgacatcga tatagtagct taccttatca
     7021 gtgtaagttt ggtttcttaa cataaaatga gttgaagcta attcatattc aaatttacca
     7081 gtttgatcat aattcagtgt tactatacat ggtactgctt ctcgtagttc gacacgtccg
     7141 acatcataaa tgacgtctct aacagcaccg ctataggcgc gaattaaacc gccaccacct
     7201 aatttaatac caccaaaata tcttgttact acgacacacg cattatgaac atcgagcttt
     7261 tttaatatgt ctaacattgg aacaccggca gttcctgtcg gttcaccatc atcattcgct
     7321 ttttgaatat tcatttcagg tccaatagta tatgcagaac aattatgagt ggcatcttta
     7381 tgttcttttt ttattgcagc aataaatgct ttagcttcat cttcattttg aacaggtttg
     7441 atatgagcaa tgaatcttga tttactaatc acattttcaa taatgtgttc ttttttaaca
     7501 gtaatgatat tttgtgtcat aataactcct taattcataa gcttaagatt atttaatctt
     7561 cattatacac tgaaaatgat atgactataa atcgtttgat tgccattttc tttttaactg
     7621 aaatattgta tcattgcaat gagtatattt taggaggacg actatgaaaa ttgctgtgat
     7681 gaccgattct acaagttatc tgtcgcagga cttaatcgat aaatataata ttcaaatagc
     7741 gccattaagt gtgacttttg atgatgggaa gaactttaca gaaagtaatg aaatagcaat
     7801 tgaagaattt tataataaaa tggcatcgtc tcaaacgatt ccaacaacaa gccaaccagc
     7861 aattggcgaa tggattacta aatatgaaat gctaagagat cagggttaca cagatatcat
     7921 tgtcatttgc ttatcaagtg gaattagtgg aagttatcaa tctagttatc aagcagggga
     7981 aatggttgaa ggtgttaatg tacatgcatt tgatagtaaa cttgcagcaa tgattgaagg
     8041 atgttatgta ttacgtgcta ttgaaatggt tgaagaagga tacgaaccac agcaaattat
     8101 tgatgattta actaatatgc gtgaacacac aggtgcatat ttaattgttg atgatttaaa
     8161 gaatttacaa aaaagtggtc gaattactgg tgctcaagca tgggttggaa cattattgaa
     8221 aatgaagcca gttcttaagt ttgaagatgg caagattata ccagaagaaa aagttcgtac
     8281 taaaaagcgt gccattcaaa cattagaaaa gaaagtatta gatattgtaa aagactttga
     8341 agaagtaact ttatttgtca taaatggaga tcatttagaa gatggtcaag cgttatacaa
     8401 aaaattacaa gaagattgtc cttcagctta tcaagtagca tactctgagt ttggtccagt
     8461 tgttgcagca catttaggtt ctggtggatt aggtttaggc tatgttggca gaaaaataag
     8521 attaacataa ttataaaatt ttaataaaag agtctatatt gtaattggaa attatctctc
     8581 gtatacatgg ctttaaatgt tcatcatttg aaagtcaaaa tactaaagat ataagaaaat
     8641 cattataata ttaggctctt ttttacgttg aaatgaggtt ttaagcatta aacattacgg
     8701 gaaattaatt catcctcata cttcacttac taatgaaaaa attaaaaaag aagtaacagg
     8761 tgtcatcaaa caaaagtcaa actattattg tgttcaatgt gaaagtacaa atccaaagca
     8821 tttttatcag tatgattcct cagtacattc caagaaaatt gtatattgca gaaattgtat
     8881 atcactgggt cgaatggata atgtaacaag atataaaata acagaaagtt cgcaaagttc
     8941 atcacaagca tattatcatc tctcatttga attgtcggaa caacagtctt atgcctcaga
     9001 acatattgtt cgagccatta gaatgagaca aacgattttg ttatatgcag taacaggagc
     9061 aggtaagaca gaaatgatgt tccaaggcat tcaatatgca agacgacagg gagataatat
     9121 agctattgtg tcaccacgtg tagatgttgt tgtagaaatt agtaaacgta ttaaagacgc
     9181 atttcttaat gaagatatag acatactaca ccagcaatca agtcaacaat ttgaagggca
     9241 ttttgttgta tgcacagtgc atcaacttta ccgattcaaa cagcactttg atactatttt
     9301 tattgatgaa gtcgatgcct ttcctttatc aatggataaa agtttacaac aagcattgaa
     9361 gtcatcttct aaaattgaac atgcaacgat ttatatgaca gcaacaccac cgaaacaact
     9421 tctgtcagag attccccacg aaaatataat taaattgcca gctcgctttc ataaaaaatc
     9481 acttccagtt cctaaatatc gctatttcaa acttaataat aataagattc agaaaatgtt
     9541 atatcgaatt ttacaagatc aaattaataa tcaacgttat acactggtgt tttttaacaa
     9601 tatagaaaca atgattaaaa cattttcggt ttataagcag aaaattacta aattaacata
     9661 tgtccatagc gaggatgttt ttcgctttga aaaagttgaa caattaagga atggacaatt
     9721 cgatgtcatt tttactacga caatattaga acgtggattt acaatggcaa atttagatgt
     9781 tgttgttatc gatgcacatc aatatactca agaggcttta atacaaattg ctggacgtgt
     9841 tggacgaaaa ttagaatgtc ctactggaaa agtattgttt tttcatgaag gtgtatgtat
     9901 gaatatgatt caagctaaaa aagagattca aaagatgaac aaattagcat taaaaagagg
     9961 ttggattgat gaataattgt ttgagttgtg gtgctgagtt atatgaaaag ataaccattt
    10021 ataatttgtt caagaaacct aatagattat gtgacagatg caaagagaat tgggacaata
    10081 ttaaacttga tattaaagca aggcgatgtt caaggtgctt aaaatactta aatcaaaatg
    10141 aagcgtattg tttagactgc aagtttctat cggcacactt taatttaatg gaacaattat
    10201 attgtcaatt tcaatatgac ggtataatga aagagatgat acatcagtat aaattttcga
    10261 aagactatta tttatgtgaa ttattggcac atttgattga aataccacaa acatcttatg
    10321 actatattgt gccaattcct tcttcgccgg cacatgattt atctagaaca tttaacccgg
    10381 tagaagcagt actaaaagct aaagggattc tctttgataa gattttaaag atgtcaaata
    10441 gaccaaaaca gtctcattta actaagaaag agcgtctggc agatgaaaat ccatttatta
    10501 ttgatatgga attagattta aacggaaagg aaatattact cgttgacgat atttatacaa
    10561 ctggattaac aattcatcgt gcagggtgta aattatatgc taaaaatatc agaaaattca
    10621 aagtgtttgc gtttgcacga tagcgtaaaa atgttaaaat ataataaaga gttaccaata
    10681 aagaggttta aggagagatt actatgatta gatttgaaat tcatggagat aacctcacta
    10741 tcacagatgc tattcgcaac tatattgagg aaaaaattgg taagttggaa cgttatttta
    10801 atgacgtacc aaatgcagtg gcgcatgtta aagttaaaac ttattcaaat tcagctacta
    10861 aaattgaagt aacaattcca ttgaaaaatg ttacgttaag agctgaagag cgaaacgatg
    10921 atttatacgc aggtattgat ttaattaata ataaacttga aagacaagtt cgaaaatata
    10981 aaacacgtat taatcgtaag agccgtgatc gaggagatca agaagtgttt gttgccgaat
    11041 tacaagaaat gcaagaaaca caagttgata atgacgctta cgatgataac gagatagaaa
    11101 ttattcgttc aaaagaattc agcttaaaac caatggattc agaagaagcg gtattacaaa
    11161 tgaatctatt aggtcatgac ttctttgtat tcacagacag agaaactgat ggaacaagta
    11221 tcgtttaccg ccgtaaagac ggtaaatatg gcttgattca aactagtgaa caataaatta
    11281 agtttaaagc acttgtgttt ttgcacaagt gcttttttat actccaaaag caatttctaa
    11341 ctatttcata gttcgataat gtaatttgtt gaatgaaaca tagtgactat gctaatgtta
    11401 atggatgtat atatttgaat gttaagttaa taatagtatg tcagtctatt gtatagtccg
    11461 agtcgaaaat cgtaaaatat ttataatata atttattaga aagtataatt gcgtattgag
    11521 aatatattta ttaaggataa acttgttgac aacagaatgt gaatgaagta tgtcataaat
    11581 atatttatat tgattctaca aatgagtaaa taagtataat tttctaacta taaatgataa
    11641 gatatattgt tgtaggccaa acagtttttt agctaaagga gcgaacgaaa tgggattttt
    11701 atcaaaaatt cttgatggca ataataaaga aattaaacag ttaggtaaac ttgctgataa
    11761 agtaatcgct ttagaagaaa aaacggcaat tttaactgat gaagaaattc gtaataaaac
    11821 gaaacaattc caaactgaat tagctgacat tgacaatgtc aaaaagcaaa atgattattt
    11881 agataaaatt ttaccagaag catatgcact tgttagggaa ggctctaaac gtgtattcaa
    11941 tatgactcca tataaagttc aaattatggg tggtattgca attcataaag gtgatatcgc
    12001 tgagatgaga acaggtgaag gtaaaacgtt aacagcgaca atgccaacat acttaaatgc
    12061 attagctggt agaggtgttc acgttattac agtcaatgaa tacttatcaa gtgttcaaag
    12121 tgaagaaatg gctgagttat ataacttctt aggtttgact gtcggattaa acttaaacag
    12181 taagacgact gaagaaaaac gtgaagcata tgcgcaagac attacttaca gtactaacaa
    12241 tgagttaggt tttgactatt taagagataa catggtgaat tattctgaag atagggtaat
    12301 gcgtccatta catttcgcaa tcattgatga ggttgactca attttaattg atgaggcacg
    12361 tacgccatta attatttctg gtgaagctga aaagtcaacg tcactttata cacaagcaaa
    12421 tgtttttgcg aaaatgttaa aacaggacga agattataaa tatgatgaaa aaacaaaagc
    12481 agtacattta acagaacaag gtgccgataa agctgaacgt atgttcaaag ttgaaaactt
    12541 atatgatgta caaaatgttg atgttattag tcatatcaac acagctttac gtgcgcacgt
    12601 tacattacaa cgtgacgtag actatatggt tgttgatggc gaagtattaa ttgtcgatca
    12661 atttacagga cgtacaatgc caggccgtcg tttctctgaa ggtttacacc aagctattga
    12721 agcgaaagaa ggcgttcaaa ttcaaaatga atctaaaact atggcgtcta ttacattcca
    12781 aaactatttc agaatgtaca ataaacttgc gggtatgacc ggtacagcta aaaccgaaga
    12841 agaagaattt agaaatattt ataacatgac agtaactcaa attccgacaa ataaacctgt
    12901 gcaacgtaat gataagtctg atttaatcta cattagccaa aaaggtaaat ttgatgcagt
    12961 agtagaagat gttgttgaaa aacacaaggc aggacaacca gtgctattag gtactgttgc
    13021 agttgagact tctgaatata tttcaaattt acttaaaaaa cgtggtatcc gtcatgatgt
    13081 gttaaatgcg aaaaatcatg aacgtgaagc tgaaattgtt gcaggcgctg gacaaaaagg
    13141 tgccgttact attgccacta acatggctgg tcgtggtaca gatattaaat taggtgaagg
    13201 tgtagaggaa ttaggcggtt tagcagtaat aggtacagag cgacatgaat ctcgtcgtat
    13261 tgatgaccag ttacgtggtc gttctggacg tcaaggtgat aaaggggata gtcgcttcta
    13321 tttatcatta caagatgaat taatgattcg ttttggttct gaacgtttac agaaaatgat
    13381 gagccgacta ggtttagatg actctacacc aattgaatca aaaatggtat caagagctgt
    13441 agaatcagca caaaaacgtg tagaaggtaa taacttcgac gcgcgtaaac gtatcttaga
    13501 atacgatgaa gtattacgta aacaacgtga aattatctat aacgaaagaa atagtattat
    13561 tgatgaagaa gacagctctc aagttgtaga tgcaatgcta cgttcaacgt tacaacgtag
    13621 tatcaattac tatattaata cggcagatga cgagcctgaa tatcaaccat tcatcgacta
    13681 cattaatgac attttcttac aagaaggtga cattacagag gatgatatca aaggtaaaga
    13741 tgctgaagat attttcgaag tcgtttgggc taagattgaa gcagcatatc aaagtcaaaa
    13801 agatatctta gaagaacaaa tgaatgagtt tgagcgtatg attttacttc gttctattga
    13861 tagccattgg actgatcata tcgacacaat ggatcaatta cgtcaaggta ttcacttacg
    13921 ttcttatgca caacaaaatc cattacgtga ctatcaaaat gaaggtcatg aattatttga
    13981 tatcatgatg caaaatatcg aagaagatac ttgtaaattc attttaaaat ctgtagtaca
    14041 agttgaagat aatattgaac gtgaaaaaac aacagatttt ggtgaagcga agcacgtttc
    14101 agctgaagat ggtaaagaaa aagtgaaacc aaaaccaatc gttaaaggcg atcaagtagg
    14161 tcgtaacgat gattgtccat gtggtagtgg taaaaaattc aaaaattgcc atggaaaata
    14221 aatgatataa aataactcct tccaattaaa cacctatagt ttgtgttatg ggaggagtct
    14281 ttttatttta caagcattaa atactttaaa aaatgtgaag aagttgttaa acgttgttat
    14341 gtacttagtt ttaaaaaaat aggtttaggc atttgtcgat gataaatgta ctgattttta
    14401 acaataaatg cataaactaa ttgtcagtgt gcttatattt tttaacattg ttatttaaca
    14461 aaattatgtt aaaatttagc attataaaag atacaaatca atgacttgaa ttgaattata
    14521 aataggagcg aaggctatgg aattatcaga aatcaaacga aatatagata agtataatca
    14581 agatttaaca caaattaggg ggtctctttg acttagagaa caaagaaact aatattcaag
    14641 aatatgagga aatgatggca gaacctaatt tttgggataa ccaaacgaaa gcgcaagata
    14701 ttatagataa aaataatgcg ttaaaagcaa tagttaatgg ttataaaaca ctacaagcag
    14761 aagtagatga catggatgct acttgggatt tattacaaga agaatttgat gaagaaatga
    14821 aagaagacct agagcaagag gtcattaatt ttaaggctaa agtggatgaa tacgaattgc
    14881 aattattatt agatggacct cacgatgcca ataacgcaat tctagagtta catcctggtg
    14941 caggtggcac ggaatctcaa gattgggcta atatgctatt tagaatgtat caacgttatt
    15001 gtgagaagaa aggttttaaa gttgaaactg ttgattattt acctggagat gaagcaggga
    15061 ttaaaagtgt aacattgctc atcaaagggc ataatgctta tggttattta aaagctgaaa
    15121 aaggtgtaca ccgactagta cgaatttctc catttgattc atcaggacgt cgtcatacat
    15181 catttgcatc atgtgacgtg attccagatt ttaataatga tgaaatagag attgaaatca
    15241 atccggatga tattacagtt gatacattca gagcttctgg tgcaggtggt cagcatatta
    15301 acaaaactga atcggcaata cgaattaccc accacccctc aggtatagtt gttaataacc
    15361 aaaatgaacg ttctcaaatt aaaaaccgcg aagcagctat gaaaatgtta aaatctaaat
    15421 tatatcaatt aaaattggaa gagcaggcac gtgaaatggc tgaaattcgt ggcgaacaaa
    15481 aagaaatcgg atggggaagc caaattagat catatgtttt ccatccatac tcaatggtga
    15541 aagatcatcg tacgaacgaa gaaacaggta aggttgatgc agtgatggat ggagacattg
    15601 gaccatttat cgaatcatat ttaagacaga caatgtcgca cgattaatat atattttaag
    15661 ggagtgggac agaaatgata ttttcgaaaa atttatttcg tcgtcccacc ccaacttgca
    15721 ttgtctgtag aaattgggaa tccaatttct ctttgttggg gccccgccgg caaggatgac
    15781 tagaattgaa aaaagtttgt tacaagcgta ttttcgttca gtcaactact gccaatataa
    15841 ctttgtagag cattgaacat tgatttatgt ctcaggcttt tttgttttct taaaggtagc
    15901 taaataaatt gtaaattaga ttttggaata tgatttgttt atgaatattt aagtacaatt
    15961 cggtggtttt tgacgaaata ttatccttta taaaagtttc gtttgttaaa caaatctatg
    16021 tactgtgcat taagtaatat taccttcaac gaatttcaat gtattgaatt aaatgttgaa
    16081 aacgttgtca atcatttgca gtgaatacat tttacaccga gtaggtagtt tgcattcttt
    16141 tttatattgt taaccatttg attacatcgt tataacaata gcttttgaca aaatgtattg
    16201 tgctatagta tttgcatact taaaatacta acagcaaagg aatgacagca agatgaaaaa
    16261 atctcttaca gtgacggttt cgtcagtgtt agctttttta gctttaaata atgcagcaca
    16321 tgcacaacaa catggcacac aagtaaaaac acctgttcaa cataattatg tatcaaatgt
    16381 tcaagcacaa acgcaatcac cgacaactta tacagtagtt gctggcgatt cattatataa
    16441 gattgcttta gagcatcact taatgttgaa tcaattatat tcatacaatc ctggtgtaac
    16501 acctttaatt tttcctggtg acgtgatttc acttgtgcct caaaataaag tgaaacaaac
    16561 taaaacggtt aaatcaccag taagaaaagc aagccaagct aaaaaggtag taaaacaacc
    16621 tgtacaacaa gcgtctaaaa aagtagtagt taagcaagca cctaagcaaa cagtagctaa
    16681 gacagttaat gtagcataca aacctgctca agtacaaaaa tcagtaccaa ctgtacctgt
    16741 tgcacataac tacaataaat cagttgctaa cagaggaaac ttatatgctt atggaaactg
    16801 cacatattat gctttcgatc gtcgtgcaca attaggtaga agtataggaa gtttatgggg
    16861 caatgcaaat aactggaatt acgcagcaaa agttgcagga tttaaagtag ataaaacacc
    16921 agaagttggc gctattttcc aaacagctgc tggaccatat ggacatgttg gtgttgttga
    16981 atctgtaaat cctaatggaa caattactgt ttcggaaatg aactatgctg gatttaatgt
    17041 taaatcttca agaacaattt taaatccagg aaaatataat tacatccact aagtaacata
    17101 tcaagacaag actatcctct tagcctgttt aagtaacagg ttgagaggat tttttggtat
    17161 catctaatca gacttatata aagaagatat ttaaatgata tttaattgaa tgatatgtaa
    17221 aaagaaagta taatttatga ttaattaatg gaggaggtaa ttgaaatggg tgtacatcaa
    17281 tattttaaaa gattat
//
LOCUS       DAPIDM010000043        17241 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000043 DAPIDM010000000
VERSION     DAPIDM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.043"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            97..2193
                     /gene="coa"
                     /locus_tag="RJE42_001273"
     CDS             97..2193
                     /gene="coa"
                     /locus_tag="RJE42_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDV5876702.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GKQIADGYYWGIIENLENQFYNIFHLLDQHKYAEKEYKDAVDKLKTRVLEEDQYLLER
                     KKEKYEIYKELYKKYKKENPNTQVKMKAFDKYDLGDLTMEEYNDLSKLLTKALDNFKL
                     EVKKIESENPDLRPYSESEERTAYGKIDSLVDQAYSVYFAYVTDAQHKTEALNLRAKI
                     DLILGDEKDPIRVTNQRTEKEMIKDLESIIDDFFIETKLNRPQHITRYDGTKHDYHKH
                     KDGFDALVKETREAVSKADESWKTKTVKKYGETETKYPVVKEEKKVEEPQSPKVSEKV
                     DVQETVGTTEEAPLPIAQPLVKLPQIGTQGEIVKGPDYPTMENKTLQGVIVQGPDFPT
                     MEQNRPSLSDNYTQPSVTLPSITGESTPTNPILKGIEGNSSKLEIKPQGTESTLKGIQ
                     GESSDIEVKPQATETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPR
                     FNKPSETNAYNVTTHANGQVSYGARPTQNKPSETNAYNVTTNQDGTVSYGARPTQNKP
                     SKTNAYNVTTHANGQVSYGARPTYNKPSETNAYNVTTHANGQVSYGARPTYKKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNV
                     TTHADGTATYGPRVTK"
     gene            complement(2375..2482)
                     /locus_tag="RJE42_001274"
     CDS             complement(2375..2482)
                     /locus_tag="RJE42_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876703.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVCHIDVRGLF"
     gene            complement(2780..3964)
                     /locus_tag="RJE42_001275"
     CDS             complement(2780..3964)
                     /locus_tag="RJE42_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDV5876704.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQLDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTIEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(3994..6255)
                     /locus_tag="RJE42_001276"
     CDS             complement(3994..6255)
                     /locus_tag="RJE42_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDV5876705.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIKIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAKAFNKKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLYENGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENEHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRHGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(6441..7652)
                     /locus_tag="RJE42_001277"
     CDS             complement(6441..7652)
                     /locus_tag="RJE42_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDV5876706.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLVHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(7764..9269)
                     /locus_tag="RJE42_001278"
     CDS             complement(7764..9269)
                     /locus_tag="RJE42_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDV5876707.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYD
                     KILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(9295..10872)
                     /locus_tag="RJE42_001279"
     CDS             complement(9295..10872)
                     /locus_tag="RJE42_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV5876708.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGVAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     IDDETYLHYTFPSVDVALLRGTYADQQGNIYLTQESYLSECYHVALNAKANHGKVIVQ
                     VKDLVDEYQLNPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGELKQSIHKV"
     gene            11231..11902
                     /locus_tag="RJE42_001280"
     CDS             11231..11902
                     /locus_tag="RJE42_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDV5876709.1"
                     /translation="MRVNVLQHTPNEGVGYIGEWAKERGHEVYIYHPYMNDGVLPLAE
                     ETDMLVILGGPMNPDDEMPWIQSERQLILVLMAQNKPIYGACFGAQQISKALGYAVLK
                     SPVKEVGWDDVYLESEIIPNIPSRLKALHWHEDMFQIPQQAELLFSGNHIRNQGFIID
                     HHVIGLQFHFEPGPDDVREIVLNDAQYINHSILEQSAEDILAFEVPKQNRQAIFRILD
                     FITQA"
     gene            12128..13270
                     /locus_tag="RJE42_001281"
     CDS             12128..13270
                     /locus_tag="RJE42_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDV5876710.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFASLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIVKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTI
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(13586..15061)
                     /locus_tag="RJE42_001282"
     CDS             complement(13586..15061)
                     /locus_tag="RJE42_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5876711.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTSSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKAHIEIDIKSVDDIESYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNNNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(15259..15615)
                     /locus_tag="RJE42_001283"
     CDS             complement(15259..15615)
                     /locus_tag="RJE42_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV5876712.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLYGAFKEKYEKELRDNETQKEAFEKLKDIVNQHNHVLLLYAAK
                     DTEHNQAVVLQQLLNT"
     gene            complement(15772..15945)
                     /locus_tag="RJE42_001284"
     CDS             complement(15772..15945)
                     /locus_tag="RJE42_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876713.1"
                     /translation="MKLNQRYVKVFALYFASIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(15970..17115)
                     /locus_tag="RJE42_001285"
     CDS             complement(15970..17115)
                     /locus_tag="RJE42_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDV5876714.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETDAYDFSQYVPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EDASAKGLDTQMVQVAENEQHLPFKDNFNSIANHYDNAKLYTHLKGEQGYIGAEELKA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
BASE COUNT         5539 a         3084 c         2995 g         5623 t
ORIGIN      
        1 ctttgtttat tctagttaat atatagttaa tgtcttttaa tattttgttt ctttaatgta
       61 gattgggcaa ttacattttg gaggaattaa aaaattatga aaaagcaaat aatttcgcta
      121 ggcgcattag cagttgcatc tagcttattt acatgggata acaaagcaga tgcgatagta
      181 acaaaggatt atagtgggaa atcacaagtt aatgctggga gtaaaaatgg gaaacaaatt
      241 gcagatggat attattgggg aataattgaa aatctagaga accagtttta caatattttt
      301 catttattgg atcagcataa atatgcagaa aaagaatata aagatgcagt agataaatta
      361 aaaactagag ttttagagga agaccaatac ctgctagaaa gaaaaaaaga aaaatacgaa
      421 atttataaag aactatataa aaaatacaaa aaagagaatc ctaatactca ggttaaaatg
      481 aaagcatttg ataaatacga tcttggcgat ttaactatgg aagaatacaa tgacttatca
      541 aaattattaa caaaagcatt ggataatttt aagttagaag taaagaaaat tgaatcagag
      601 aatccagatt taagaccata ttctgaaagt gaagagagaa cagcatatgg taaaatagat
      661 tcacttgttg atcaagcata tagtgtatat tttgcctacg ttacagatgc tcaacataaa
      721 acagaagcat taaatcttag ggcaaaaata gatttgattt taggtgatga aaaagatcca
      781 attagagtga cgaatcaacg tactgaaaaa gaaatgatta aagatttaga atctattatt
      841 gatgatttct tcattgaaac aaagttgaat agacctcaac acattactag atatgatgga
      901 actaaacatg attaccataa acataaagat ggatttgatg ctctagttaa agaaacaaga
      961 gaagcggttt ctaaggctga cgaatcttgg aaaactaaaa ctgtcaaaaa atacggggaa
     1021 actgaaacaa aatatcctgt tgtaaaagaa gagaagaaag ttgaagaacc tcaatcacct
     1081 aaagtttctg aaaaagtgga tgttcaggaa acggttggta caactgaaga agcaccatta
     1141 ccaattgcgc aaccactagt taaattacca caaattggga ctcaaggcga aattgtaaaa
     1201 ggtcccgact atccaactat ggaaaataaa acgttacaag gtgtaattgt tcaaggtcca
     1261 gatttcccaa caatggaaca aaacagacca tctttaagtg acaattatac acaaccatct
     1321 gtgactttac cgtcaattac aggtgaaagt acaccaacga accctatttt aaaaggtatt
     1381 gaaggaaact catctaaact tgaaataaaa ccacaaggta ctgaatcaac gttgaaaggt
     1441 attcaaggag aatcaagtga tattgaagtt aaacctcaag caactgaaac aacagaagca
     1501 tcacattatc cagcgagacc gcaatttaac aaaacaccta aatatgttaa atatagagat
     1561 gctggtacag gtatccgtga atacaacgat ggaacatttg gatatgaagc gagaccaaga
     1621 ttcaataagc catcagaaac aaatgcatat aacgtaacaa cacatgcaaa cggccaagta
     1681 tcatacggag ctcgcccaac acaaaacaag ccaagtgaaa caaatgcata caacgtaaca
     1741 acaaatcaag atggcacagt atcatatggc gctcgcccga cacaaaacaa gccaagcaaa
     1801 acaaacgcat ataacgtaac aacacatgca aatggtcaag tgtcatacgg agctcgtccg
     1861 acatacaaca agccaagcga aacaaatgca tataacgtaa caacacatgc aaacgggcaa
     1921 gtatcatacg gagctcgtcc gacatacaag aaaccaagcg aaacgaacgc atataacgta
     1981 acaacacatg caaacggcca agtatcatat ggcgctcgcc caacacaaaa caagccaagt
     2041 aaaacaaatg catataacgt aacaacacat gcaaatggtc aagtgtcata cggagctcgt
     2101 ccgacataca agaagccaag cgaaacaaat gcatacaatg taacaacaca tgcagatggt
     2161 actgcgacat atggtcctag agtaacaaaa taagtttata actctatcca cagacataca
     2221 gtcaatacaa aacattacgt atctttacaa cagtaatcat gcattctatg atgcttctaa
     2281 ctgaattaaa gcatcgaaca atcggaagca tatttctaaa ttatttattc attatagtct
     2341 taaacataac atgacctaat atattactaa cctattaaaa taaaccacgc acatctatgt
     2401 gacatacgac aatcacagca ataataattg ctttagaaag tcgtaccgaa ctggaactta
     2461 caagtctagt tcgaacacac actgatgtga gtggttttct ttattttaaa catgaacaat
     2521 cagataagtt actagcatta gcaaatatta ttaaatcaaa gggcttcgat tcataaaatt
     2581 taaaacaatg attaaaatta gacgtgtaaa tgttaaattc taaaacggaa atacccgcta
     2641 tcctattaaa ccactttttt gttcaatcac tatatttcac acagcttcaa taataaaatg
     2701 aaactgcttc aatccgcttc aacttcagtc tacttcattt aataacaaaa cgaatccgct
     2761 tcatccaaaa tcaaccattc taacgcacat actcaaatat agcagctgca cccataccga
     2821 caccaataca catcgtaacc atgccgtaac ggctatcggg acgtctaccc atttcattaa
     2881 gtaaacgcgc ggttaacatt gcgcctgtag cacctaatgg atgacctaaa gcaatagcgc
     2941 caccattcac attcgtacgt gatatatcta gacctacttc tttaatagat gcaatcgttt
     3001 gagaagcaaa tgcttcgttc aattcgatca aatcaatgtc ttcaacagat agattgctga
     3061 gtgacaatac ttcaggaatc gcatatgcag gcccaatacc cataattttc gggtcaacgc
     3121 ctactgcctt ataaccaacg aatcgtgcaa taggtgtcac gccgagttct ttcactttat
     3181 ctccagacat taaaactaca aatcctgcgc catcagaaag tggggcagat gttcccgcag
     3241 tcacagtgcc gtcagcttta aatactgtac gtaacttggc taatgcctct atcgtggtgt
     3301 caggtcgtat aaattcatct tggtcaaaga tatttgtgtg tacttttggt cctgcatttg
     3361 tatattcaac tgagtttact tgtattggaa taatttcatc tttgaaccga ccatcaagtt
     3421 gtgcgtcata ggcacgttga tgacttctga cagcataagc atcttgatct tcgcgtgata
     3481 cgtcaaattg ggatgctaca ttttcagcag ttaaacccat aggatatgac gcacctatat
     3541 catcatattg taaggtagga ttgtttgtgg gctcgttgcc acccattggt acggcactca
     3601 tcaattcaac gccaccagct acaagtatat ctccttgacc agccataatt tgattggctg
     3661 caatcgcgat ggtttgtaat cctgatgagc agtagcgatt cactgtttga cccggtaccg
     3721 tgtcagataa tcccgtacgc aatgcaatcg ttcgtgcaat gttttgtcct tgtaatcctt
     3781 ctggaaaagc cgtaccaaca atgacatctt caatcatatt cttattgaat tttccgtcaa
     3841 tacgtttcaa tacgccttgt aatactttgg ctgcgacatc atcaggtctt tcgtggaata
     3901 atgcgccttg ctttgctttc gctgcggctg aacgcccata agctacaatg tatgcttctt
     3961 gcatggttat catcctctct taatgactat cttttaatta cgtaatggct taccagtttt
     4021 taacatatgt gcaattcttt catatgattt tttagatttt agtaagtcaa taaagccaat
     4081 tttctccaac gattgaatgt aacgttgatt gataaatgta tttcttggta aatcaccacc
     4141 tgctaaaatt gtggcgatat ttaaggcgat atgataatca tggtcgctaa taaaatgacc
     4201 gtgtctttgc gcatctaatt gtccttggat caatgctttg aagtcttcgc ctaaagcgat
     4261 atattgatgt ctaggattcg gaatatagtt tgtttctgct tcatatttcg cacgtttgag
     4321 cgcaacttcg acacgttgtg ctgtattgaa aataatcgta tctgtatcac gtaaataacc
     4381 ataacgacgt gcctcaaagg catttgtaga gactttcgca aatgcgatat tcgtcagtac
     4441 ttttgtcatg gaagcttgtt tgtcatcaaa cttatgcgat gtgcgtaata tgcgatcagc
     4501 catttctgca aggccaccgc cactcggtaa taagccaaca cctgcttcaa caagaccgat
     4561 ataagtttca cttgcagcga caacaatagg tgagtaaagt acaagctcac agccaccgcc
     4621 taaggcacga ccttgaacag ctgtgactac tggtttcaaa ctatacttca aacgattaaa
     4681 gctataatgt aatttatcaa ttgattgtgc aacgacatca tctacaagac catcttcatg
     4741 cgcctttttc attaagaaaa ggttagcacc cacactgaaa ttattaccat ctgcataaat
     4801 aaccatactt gtgtaatgtt cattttccag taaatcaatc gcatcaacta acgcatcgtt
     4861 gaattcatcg gtaatgacat tatttttact ttgtaatttc agtaacagtt gatcatcatg
     4921 agttacggaa agtttggcat cacctttatc ccaaagttca tcttttacga agtgagaaat
     4981 aggcgttgca tattcaatgg tctcatcttg tttataaaag ccaccgtcta aatcactaat
     5041 ccattgtggt aagtctccaa gttcgtcttc catacgtgtt ttaacacgtt cgtatcccat
     5101 tgcatcccat aattggaatg gaccaagttt ccagttgaac ccccagacaa gcgcacggtc
     5161 tatgtctcgg aaatcatcgg tagctttagg tacattgata gcagagtaat agaaattatt
     5221 acgtaatgtc tcccataaaa atagtcccgc ttcgtcttgc gcattgaata tgacatcaag
     5281 gttatgcact aagtctttat taaattcatt taaaattggt aattgtggtt gcgatacagg
     5341 tacataatct tgtttttcaa catcgtaaac aagtcgagct ttagtttctt tatccttttt
     5401 gtaaaatcct tgttttgttt tacgtccgag tgcgccattt tcatacaacg tatttacaat
     5461 tttgacatca tgaaaataag gtgtttcttc aggtacttgt tgcatgcctt taattacaga
     5521 cactgcaata tctaaaccga ctaggtcaga tagggcatat gtacctgttt taggacgacc
     5581 aatcgcttgc ccagttaaag catctacatc tacaatgctt aacttgtgtt gctcggcgcg
     5641 atacataata tcattcattg tttgcgtgcc gactctattt gcgacaaagc caggcacatc
     5701 attgacgaca atgacacctt tacctaacac attttgcgcg aaatttttta catctaatat
     5761 aatagattcc ttcgtgtgtg acgtaggtat taactccact aatttcataa tacgtggtgg
     5821 gttaaagaaa tgtagaccaa agaatcgttc ttgatccttc ttgttaaatg ctttagcaat
     5881 cgcattaatt ggaatacctg atgtatttgt ggcgaataaa gcatcttctt tagcatgttg
     5941 tagaacttgt tgccaaacag catgcttaat tttgatatct tctttgactg cttcgatata
     6001 taaatcagca tcatcattta ccaagtcatc atcaaaatta ccatatgtta aatgactcgc
     6061 tagatttaag tcgaatagaa gcggccgttt cttatctgta attttatcgt aagatttttt
     6121 cgcaatgaga tttggatcgt ttttgtccac tacaatatct aatagtttta ctttaagtcc
     6181 agcattcaca aagagtgctg ccagttgagc gcccattgtg cctgcgccaa gaacggttac
     6241 tttattaatt gtcatagtga ttcctccaat ttagttgagg ataagataac cattaagata
     6301 attggaataa cgttgctatt ttataaaatt aattaagtat ctttgacagt catcttagcc
     6361 tcttatttaa ggaaaaagct ttatgcttaa aataagtctt ttttagtgaa attactgcat
     6421 cacatataat tctttgctat ttatacgaaa gcagaatctc cagtcaaagc gcgtccaatt
     6481 actaaggcat taatttcatg tgtgccttcg tatgtgtaaa tcgcttctgc atcagagaag
     6541 aaacgtgcaa tatcataatc gtcagctagt atgccattac cacctgtaat accacggccc
     6601 atagctactg tctcacgcaa acgtaaggca ttcatcatct tcgccgttga agttgcaacc
     6661 tcgtcatatt caccatgtgc ttgcatatta gctaattgag cacatgttgc cattgcttga
     6721 gctaaattac cttgcatcat tgctagcttt tcttgtatta actgatattt actaattggt
     6781 ttgccgaatt gcttacgctc agtgacataa tctaatgtgg cacgtaaagc gccagccata
     6841 ccacctgtag ccatataagc aacgcctgct cttgttgaat aaagaatttt ggcaatatct
     6901 ttaaagcttg ttatgttttg taagcgatcc gcttcatcta ctttgacatt agttaattta
     6961 attagtgcgt taggaacaat gcgaagtgcg attttattat caatgacttc aatatcgacg
     7021 ccatcttgtt ctggtctgac tacaaagcaa tggggtttgc cagtttcttt atttactgcg
     7081 aatactggaa tgacatcaga tacatgtgca ccaccaatcc atttcttttc accattgata
     7141 acccaagtat cgccctggcg ttcagcgact gtttcaagac ctcccgcaac gtccgaaccg
     7201 tgttctggtt cagttaaagc aaagcatgta cgcagttcat gtgactgtaa tttaggtaca
     7261 tatttcgcaa tttgttcttt gctacctccg aaatagaaag tgttatgcac taaaccttga
     7321 tgaacaccga gtagggtagc taaggaaata tcaaatcgcg cgagtaggta agacatgaaa
     7381 aactgaaata gttgactagg cattttggcg tttggacgat ccttgtatag taatggattg
     7441 ttaaaataat ttaattctcc cagatcttta aaataatcct cgggtacagt agcgtctatc
     7501 caatgttgat taatattttc acggtactta ctttctagca atgaatctac ttgttgtaaa
     7561 aattcgactt caccgtctgt taaaccttta gcaatactaa gtacatcttc aggaaataat
     7621 gtttttaaga ctgtttcttt ttcaaatgtc atataaattc ctcctaaaaa taatatgaat
     7681 actaatgtga aatgcattta attcaaaaac aacacgattc gcttgtaaac gcttacacaa
     7741 aatatcaaaa atttttacca catttaaagt gtttgcgaga ctttgtcatt catcatttgt
     7801 cgaatcgcaa gtttatctgg tttctgcgta ctgtttaacg gcatatgtgt cactggtaca
     7861 tacattcttg ggactttata acctgctaaa cgacttcgca tatgttgatc taaaatttta
     7921 tcgtaatgag gttcatcttc gcgaagtata atggctgcag caattgactc accatatttt
     7981 ggatgatcat agccaacgac cacacaccgg tctactagtg gatgctccgc taaagcattt
     8041 tcgacttcgg atggtaagac attttcgcca ccagttatga ttaattcttt tttgcggtca
     8101 ataataaata tatcgccatc gtcgtccatc ttcgctaaat ctcctgtcaa taagtaacga
     8161 ccatgaaacg ctttagcagt ctctgctggt ttattccaat atcctggcgt gacattttta
     8221 gccttaattg caagttcacc aatctcacca gtaggtactt cttcaccgtt atcatcaagg
     8281 atacgtgcat caacaaacat gactgcttta ccaatactca ttggtttacg ttttgaattt
     8341 tctggtgtat taacaagtac aagaggtgct tcagttaagc catagccgtt aataatgttt
     8401 atgccatatt gtttaaacgc tgcttggata cttggtaatg gttgtgaacc accttggatg
     8461 atataatcca tagctttaaa attttcagga ttaaaattac tggcacgtag cgtactataa
     8521 tacattgtcg gaatcatgat aataaatgta gggtgatatt gtgcaatcat gtcattcaat
     8581 tcttcgccgt taaagtaacg ttgaagaata agtgtgccac ctgacattaa tactggtaat
     8641 acagtatcgt taaaccctaa aacatggaac attggtgttg atacaatcgt aatatagttt
     8701 gaattgaact tgtacgtcag ctctaagttt gcgccgttat gaacaaatga ttcatatgag
     8761 aacatcacac ctttaggtga tccggttgta ccacttgtat aaattaatgc tgcaagatct
     8821 tgtggttcaa caggtgttgc ttgaaaaggt tggtgataat ctggatttac gatttcatca
     8881 tattgcgcta catcaatatc catatgcaat aagttttggt caatatcggt gagtgaactt
     8941 aaatgttttt cagcatagaa gagcagtttt aattgtgcat cttccacaat ggctgcaatt
     9001 tcttttgggt taagccgcca attcaatggt aaaaaaaccg cacctgtttt aaaacaagca
     9061 aataataaat ctaatattgc aatatcattt ggcgcaaaaa taccgataac atcgcctttt
     9121 ttaacacctt gagatgttaa ataatgtgcc atattatcag cgcgtgcatt gagttgttgg
     9181 tatgtccaag atgtttgttt tgcgtgatca ataacggcag gcttgtcatc atcgaagtct
     9241 gagcgcgttt ttatccaatc gaaattcatt agtatacccc ctttagcttc actttcatac
     9301 tttatgaatt gattgtttaa gttctcccca tttttctttg taaatgctgg tatcaattaa
     9361 ttttaaatga tcagcaataa ttggtttaaa agccatttga ttcaaaatat ctttatgcaa
     9421 atcaagacct ggtgcaattt caattagttt caagccttga ttggtgagct cgaatactgc
     9481 acgatcagta acaaaataga cttcttgctc gagtgattgt gaatattgtg cattaaagtc
     9541 gatatggctc acatctgata caaatttctg gttttgtcct tcagtttcaa tgtttaatcg
     9601 ttgattatga catgagacat gactgccagc tacaaaagta cctgaaaaga taattttatt
     9661 tacagattgt gtaatgtcta taaagccgcc acatccattt agtcggtcat tgaagtaaga
     9721 cacgttgaca ttgccgtatt gatcaacctc agcaaagcta agataggcaa ctgatacacc
     9781 attgttataa ataaaatccc atgctcgatc atgaggcatg cgcacatctg cattgtaatt
     9841 cataccaaaa tgttcacgac tcccaacgaa tccaccgaaa atgccaacat ctagaatcgg
     9901 ttgcacatca tgttcaacac attcttcatg caataaatta gagagttcat tattgatgcc
     9961 ataaccgatg ctaattgtat cgccataagt taaaaactga gcagcacgtc ggagaatcaa
    10021 cttgcgacta ttaaaaggta atgcgggttc aggtattcca tcaatccgtt cttctccaga
    10081 caaggctggt aaataatgac tctgaattac ttggcggtga ttcttttcat cttctgtgac
    10141 gtatacataa tcgacaagat ttcctgggat aacaacttca ttcggattta gttgatattc
    10201 atcaactaaa tctttaactt gtacaataac tttcccatga ttggctttcg cgtttaatgc
    10261 gacatgataa cactcgctca agtacgattc ttgagttaaa taaatgttac cttgttgatc
    10321 tgcgtatgtt cctctcagta gtgccacatc aacgctaggg aatgtgtagt gtaagtatgt
    10381 ttcatcgtcg atagttacta atgaaactaa atcatcagtt gttcgtgtat ttactttacc
    10441 gccaccgtat ctaggatcaa cagctgtgtt taatccgatt ttagtaataa ctcccggtaa
    10501 taattgatta ctctgacgat aatgagttgc aatgatacct tgtggtaaaa aataagcttc
    10561 aatgtcatta tttttcattg cttgtgccgt tttggaagaa gccgttaaaa tgctcataat
    10621 gacacgttta atcatgcgac gttctataaa atcatctaaa tccggtgcga cacctaaact
    10681 atggatatca ttcgctaata taaatgttaa atcattgggc gtatgatatg tgtcatgttg
    10741 cgctaacaca gcacgtagaa cctcggcggg taagttggct acagctaatg ctggtaaacc
    10801 aatcacatca ccatctttaa tgatatgttg taagtcgtgc catgtgattt gtttcaagca
    10861 agtcacctcc atcacatttg ataaattata gcgtttttac actttgtgta aacccttaca
    10921 agaaatataa cataacgacg tttaaaatca attagaaata tctttttatt ctgataatag
    10981 acacagtata gacacatttt aatgatcgat aacaattgta atatcaaggg tttgtaatga
    11041 attgaatatc attaaaatac ttatataaaa atattgctcg gaatataaaa agtaacccat
    11101 gtttatttaa agaatgcaaa gactaattat cattttgatg catcagtaag gcgagggtga
    11161 tcagcgtaca attagtggtg tataatgtaa ctgataaaat cattggagag ttgaaggaga
    11221 tgttgaacat atgagagtca atgttttaca acatacacca aatgaaggtg ttggatacat
    11281 aggtgagtgg gcgaaggaaa gagggcacga agtctatatt tatcatccat atatgaatga
    11341 tggcgtgcta cctcttgcgg aagaaacgga tatgttagtg atacttggtg ggccaatgaa
    11401 tccagacgat gagatgcctt ggattcaatc agagcgccaa ttaattttag tattgatggc
    11461 acaaaacaaa ccaatttatg gtgcttgctt tggtgcacaa caaatttcga aagcattagg
    11521 ttatgcggtg ttaaaatcac cagtaaagga agtaggttgg gatgatgttt acttagaatc
    11581 agaaatcatt ccgaatatac caagtcgatt gaaggcacta cattggcatg aggatatgtt
    11641 tcaaataccg caacaagcag aattattatt ttcagggaac catattagga accaaggatt
    11701 tatcatcgat catcatgtga ttggattaca atttcatttt gagcctggtc cagacgatgt
    11761 cagagaaata gtactaaatg atgcccaata catcaatcat tctattttag agcaatcggc
    11821 ggaggatata ttagcctttg aggtgcctaa acagaatagg caggccattt tccgaatact
    11881 tgattttatt actcaagcgt aagatgtgag ttgtatttta aaatgagtaa aaaaaaaacg
    11941 aatgtacaca atcgaacaaa gtagttatat aaatatatgg aaaatccatc aatattaaat
    12001 taaaatagtt ttattatgaa aagtgaaagt aggtaagtct atggaaggtc ttaatcatcg
    12061 aagaaataca gaaaacgaag agacaacaca aacgcaatca gttgcaccta atacaggtga
    12121 agaggggatg tcatcagcaa gtacacaatc aactaagacg tccgacatac ataatgaatc
    12181 tatcgataaa caaatggaag ccaaagcgca tgaaacagcg caaaatacag atttaaaaaa
    12241 tgaagcaaga agtttatttg ataatgcaac caaatcaatc ggtagactag cgggcaatga
    12301 tgaaagctta aatcttaatt taaaagatat gttttctgaa gtatttaagc cacatactaa
    12361 aaacgaagca gatgaaatat ttatagcggg tactgctaaa actacgccag caatttgtga
    12421 catatcagaa gaatggggga agccatggct cttttctcga gtattcatcg ctttcacagt
    12481 aacatttatt ggattatggg tcatggcagc gatttttaat aacacaaacg cgattccggg
    12541 tctcattttt ataggggctt taacagtacc attatcgggt ttgttcttct tttatgaatc
    12601 aaatgcgttt aaaaatatta gcatttttga agttattatc atgttcttta ttggcggcgt
    12661 attttcatta ctaagtacga tggtattata tagatttgtc gtttttagtg atcaattcga
    12721 aaggtttgct tctttaacat ttttcgatgc atttttagta ggattagttg aagaaactgg
    12781 aaaggcgctt attattgttt atttcgttaa taaattgaaa acaaataaga ttttgaatgg
    12841 attattaatc ggtgctgcta ttggtgcagg gttcgcagtt tttgaatcag caggttatat
    12901 tttgaatttc gctttagggg aaaatgtccc attattagat attgtcttca cacgtgcgtg
    12961 gactgcgatt ggtggtcatt tagtttggtc agcgattgtt ggtgctgcaa tagttattgt
    13021 gaaagaacag catggctttg aattcaaaga tatttttgat aaacgctttt taatattctt
    13081 tttatcagcc gttggtttac atggcatttg ggatacatct ttaactatac ttggcagtga
    13141 tacgttgaaa atatttattt taatcgttat tgtgtggata cttgtattca ttttaatggg
    13201 ggcaggttta aaacaagtga atttactgca gaaagaattt aaagaacaac agaaaaaagt
    13261 agacgaataa taattaaagc ttatgttgct atgtttttta gcgacataag ctttttttat
    13321 aatttgtctt taaaagagtg gaagtggaat acttttttgg agttaaaaaa gtgtttcacg
    13381 ttaaacaaat agtgacaatt aggtttatat aaaatgaata tgattcactg aaagtatgta
    13441 ataatcattt tattgaaatt catcaaacag aaagtaatac aatcatataa gcaaattaaa
    13501 ccacgccata atcatattgg atgacttcgg cgtggttttt atagttgaag cagggctgag
    13561 acataaatca atgtcttaca ctcccttatc gttcaatcgt agttcgataa tcgattaaat
    13621 agataccttc aggtgttact ttataatttt taaccttaga gttagcagcg actatttgat
    13681 cgttgtaagc aatataactg ttaggtacat ctcgacttga taatttaata atatcattag
    13741 aaatattgtg acgttcctta acatctacag tatgattcaa ttgattaatt aaatcatcaa
    13801 cgttgttatt attgtagtct cctttattaa tagcaccatc ttttttatat gcttgattaa
    13861 agaaatagcc tgtatctcca cgaggaatcg ttccgaaact atacattgtt gcatcccatg
    13921 cagaacggtc ttttaagtaa ctttctatgt catcaacact tttaatgtcg atttcaatat
    13981 gtgctttttt agcatctgat tgtaatactt gcgcaatttt cgatagctct ggacgaccgt
    14041 catacgtaat taacttaatt tttaaagggt gttcttttgt ataaccatct ttagctaata
    14101 acgtttttgc ttgttcgata ttttgtttgg ttaacttagg ttctttaata tatggaattt
    14161 tatcattaaa tggactcgtt gctggtttcg cgtaaccttg ataaatatga tctgcaatac
    14221 cttgtctatc aatgatatga tctaatgctt cacgaacgga tttagtcatt tttttattag
    14281 tatgattata cataagtaaa gaagttctaa atcctgattc ttttgacact tttaaatttt
    14341 gattgttttc tatgtcttga actttattaa ctgggacatc agttattaaa tcatcttttt
    14401 gagattctaa atttctgacg cgattattgc cgtcttcttg gtacgtcaca gtaatatgat
    14461 caagtttcgg tttaccttgc caatagtcct taaaattcga caatgatatt tttcgagatt
    14521 gcttataatc ttttatttgg taagggcctg taccaacagg agtttgatta acatctgatt
    14581 tagcatctgt atcataaatt gccataaaag gattagccaa ttcagataca agttcagggt
    14641 aagcggagct ggttttaatt gtcagttttt gaccttttgc ggtaattgat gatattggta
    14701 atgaatattt gaccaagtcg ctttttttca tgctattttc aaggctagat ttcacttttt
    14761 ctgcagtcaa tttttgaccg ttttgaaatt taatattatc ttttaattct atatctaacg
    14821 ttgtatcatt tggttgatga tacgatttca ctaatacttt ttctattttt ccttgatcat
    14881 ttgttttaaa taatgattct gcagcaccga tcttaactgg tacatctgtt tcataaggtg
    14941 caatagactt tgtttttaac ggtaacgaaa tatttaagtc tttgccagat gaatgcattg
    15001 agccacatcc tgataacact aatactgctg aaaatatagt tgctagtctt ttaaacttca
    15061 tttcattaac actctctttc taattactat gtaaaaccca ataattaata ttttaaaact
    15121 ttattttgtt aaagtaaaat gttgttcaag tttagtaatt attaaagttc aattaattgt
    15181 agtaattatg ctttttaaaa ataaatatta gaaatgaagt tagcgacatg tatagtgatt
    15241 cgcgataagc atatatacct aagtattgag caactgctgc agtaccacag cttgattatg
    15301 ttctgtatct tttgcggcat ataacaatag aacatgatta tgctgattta caatatcctt
    15361 taatttttca aaagcttctt tttgcgtctc attgtcacgc aattcttttt cgtatttttc
    15421 tttaaaagca ccataaagtt taggatcatg ttggaaccat tgtcgtaact cagtagaagg
    15481 ggcaatgtct tttaaccaat aatctaggtt agcagttctt ttcgaaatac ctcttggcca
    15541 gactctatcg actaggatac gaatagcgtc ggtattatct ttattgtcat aaatccgtcc
    15601 aatatctacg gtcatcttgt gaactccttt cttatgaaat tcagtgagca tacatcaatg
    15661 catgttgggg tgggacgacg aaataaattt tgcgaaaata tcattacagt cccactccca
    15721 attaaaaaac aaccatgaca aagtaaagtc atagctgttt ttgtatatat gttatttatt
    15781 tttacgcttg gttaaatact tcaaaccaac tgcaaagacg gtgtaaccgg caagtgtttg
    15841 aatcaatgtt ttgattaaat tgttattttt gacaacaata tttgcggtaa caatactcgc
    15901 gaaatataat gcaaatactt ttacgtaacg ttgattaagt ttcatatgag cactaacctc
    15961 ttttctcagt tatactgcaa cgcttaatct tggaataaat gtttcgtagt gtacgcgatc
    16021 catatcgtaa tttaaagatt taagtgcttc gatcatagat tgtaagaatt ttgtaccacc
    16081 acagatataa atttcaggtt tatttgctaa aaatgctttt aattcttcag caccgatata
    16141 accttgttca ccttttaagt gggtatataa tttagcgttg tcataatgat ttgcgatact
    16201 gttgaagttg tctttgaaag gtaaatgttg ttcattttca gcaacttgaa ccatctgtgt
    16261 atctaaacct ttggcagagg catcttcata cattgctact aaaggtgtga cgccaatacc
    16321 tgaacctaag aaaagttgtg gttcagtcgt attctctaat acgaatccac ctacaggcgc
    16381 agctaaatta atcatatcgc cttctttaat ctcatcgtgt aaaattgttg aaacttcgcc
    16441 ttcatgttct gttgtgacat cacgtttaac gccaaaagtt aaatgatttt tatcacctga
    16501 tacgatagaa tagtgacgtt tagctctata tggaagttta tcactagaaa catcaactgt
    16561 gatgtattgg cctggtacat attgactaaa gtcatatgca tcagtttcaa ctgtaaatga
    16621 tttaatgtct tcagattctt gtttaatatt ggtaattttg aatggtttaa aaccaatcca
    16681 catcatttga tcataaattt ctttttcaat ttggatgaac acatccgcaa taacgccata
    16741 tgctttcgcc caagcttgaa tgacagggtc attttcttct aatcctgtca cgtcttgaat
    16801 ggcttttaat aaattttccc ccacaattgg ataatgttca gcataaactt gtagtgcgca
    16861 gtgtttatat gcaactggca taataactgg tttaataaca cttaaattat cgatattaac
    16921 cgctgcggcc attactgctt gtgctaatgc tgaagattgc atgcctcgtt tttggttcgt
    16981 ttgattaaac atgtttaaaa gttcaggatg tgctttaaac atttttggat aaaagattga
    17041 cgtaatttct gtccctttct ctttaagtaa aggcaccgtt tgtttgataa tgtctttctc
    17101 ttgttctgta agcatgatac tcctccttta atctgtctac tttgattatt ctactaattt
    17161 tattgatatt caattgattg gtttgagaaa atataaataa attggcaaat ttgataattg
    17221 tatgacattt ttattttttt a
//
LOCUS       DAPIDM010000044        17043 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000044 DAPIDM010000000
VERSION     DAPIDM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.044"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(457..786)
                     /locus_tag="RJE42_001286"
     CDS             complement(457..786)
                     /locus_tag="RJE42_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876715.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVFYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            835..1572
                     /gene="sdpB"
                     /locus_tag="RJE42_001287"
     CDS             835..1572
                     /gene="sdpB"
                     /locus_tag="RJE42_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDV5876716.1"
                     /translation="MENEKKKYTFKDIAWRDLFIIPIIGVLSFALTIAAYIVPILFGY
                     YEIDSNLIFVGTITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPVFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYIKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1597..2289)
                     /locus_tag="RJE42_001288"
     CDS             complement(1597..2289)
                     /locus_tag="RJE42_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDV5876717.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2674..3471
                     /locus_tag="RJE42_001289"
     CDS             2674..3471
                     /locus_tag="RJE42_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDV5876718.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFQEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            3604..4551
                     /locus_tag="RJE42_001290"
     CDS             3604..4551
                     /locus_tag="RJE42_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDV5876719.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRDTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            5153..8107
                     /gene="fdhF"
                     /locus_tag="RJE42_001291"
     CDS             5153..8107
                     /gene="fdhF"
                     /locus_tag="RJE42_001291"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV5876720.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGQIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATFADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGESKPDWKIFQAIANKLG
                     FDWNYKHPSEIMDEIARLTPLYAGVSYERLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMLKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
     gene            8107..8580
                     /locus_tag="RJE42_001292"
     CDS             8107..8580
                     /locus_tag="RJE42_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDV5876721.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKVLDDAKILDALNGAVRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTSVRSLFGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            8875..9108
                     /locus_tag="RJE42_001293"
     CDS             8875..9108
                     /locus_tag="RJE42_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876722.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            9130..9906
                     /locus_tag="RJE42_001294"
     CDS             9130..9906
                     /locus_tag="RJE42_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDV5876723.1"
                     /translation="MKKNFKLRISTLLLIVILVVFSVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAILGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            10515..11639
                     /locus_tag="RJE42_001295"
     CDS             10515..11639
                     /locus_tag="RJE42_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDV5876724.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRPLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(11731..12684)
                     /locus_tag="RJE42_001296"
     CDS             complement(11731..12684)
                     /locus_tag="RJE42_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDV5876725.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKD
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKNNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(13047..13547)
                     /locus_tag="RJE42_001297"
     CDS             complement(13047..13547)
                     /locus_tag="RJE42_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDV5876726.1"
                     /translation="MKKLVTATTLTAGIGTALVGHAQHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(13784..14206)
                     /locus_tag="RJE42_001298"
     CDS             complement(13784..14206)
                     /locus_tag="RJE42_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDV5876727.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITFIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            14454..15536
                     /locus_tag="RJE42_001299"
     CDS             14454..15536
                     /locus_tag="RJE42_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDV5876728.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGEERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVESIGLDKLDLEKYLK"
     gene            15631..17031
                     /gene="nhaC"
                     /locus_tag="RJE42_001300"
     CDS             15631..17031
                     /gene="nhaC"
                     /locus_tag="RJE42_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDV5876729.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     AWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLSISPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIRSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAHIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKAIFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRFVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
BASE COUNT         5905 a         2373 c         3174 g         5591 t
ORIGIN      
        1 tacgtttgac atattaatca ctccacttta acgcaatata ttttattagc gttggctatt
       61 gttgtttaac gcgatggtat ttacaacttg taacgtaaga gaatgaaaaa taagctgaat
      121 atgttgtttg tttaatgata ttgaattata aaactgtaaa tttttttaag atgtatagat
      181 aaaaaatagc atactcccat gtgcaaataa tatatcaata tactgatata ttaccgtaag
      241 tcatactgta tattcccaat ttatacatta tgtactcatc tatttaaatc ttagtaaatg
      301 tagttatatt agactaaatc taccatctta atcattatga ttataactcg attaatgcat
      361 ctttacaatt tgaagaaact ttttaattgt ataatttatg taataaagtg aattttaata
      421 aataacttgt ttaaatgaca ttttatgaaa aataggttaa tgggcaaatt taattgcaaa
      481 taatgcgtta gatagttctg tatgataaaa attaagatat tgaattttgt taccatagtc
      541 gacttgtcgg aggggacgtt tattaagtag tgttataatt tcataataga aaacttgatt
      601 ttcgtttatt atatatgatg tttcaaagcc tttgaactta ttcaattcat tagtaactat
      661 ttggttaaat ttataaatat gcgtttgaat gttccaatct gtcattaaag tattatattt
      721 aatcgtatca atgatttgaa tcaaattagt aatttgttgt ctcatatact tagtttcgtt
      781 tatcatgtag tagtacctcc aaaaatcaat tattaaacag gaaaggatat aacagtggaa
      841 aatgaaaaaa agaaatacac gtttaaagat atagcatgga gagatttgtt tataattcca
      901 attataggtg tattatcatt tgcactcaca attgcagctt acatagtacc aattttgttt
      961 ggttattatg aaatcgatag taatctgata tttgtaggga ctatcacaca agctttagca
     1021 tatacgattg ggattggcgc attttattta ttccatttaa aagtgatgcc atcaaggctt
     1081 cgagctggtt ttgcgtatat caaaaaatat tggcttagat tgtcaataac gtatattatt
     1141 gctctcatct taatttatac atatgaatat atgacacagt ttctacctaa acatttgcaa
     1201 tatagtgaaa cagccaatga actggagcta aataaaatgt ttgaagtgcc tgtattttta
     1261 cctgtagcat tcttattaat tgtgattgta ggtccaatag ttgaagaaat cgtatttaga
     1321 catattttaa ttggagaatt aggtaagaag tttaatttta tagctatgag tattgtttcg
     1381 gtgttcttat ttgcatttat tcatgtgact gatgcaaagt cgccgtttga gtttggtcca
     1441 tatctcatac tatccatcat attagttttc acttatataa aatctggaag aaacctaggt
     1501 tcaacaattg cattacatat agcaaataat tttgtttctt tcgttatatc agtaataagt
     1561 atttatgcgt agataaaacc ccgtcgactc catagtttag gaatcgatgg ggtttaaaaa
     1621 ttgatgatag ttatcaatgg atatgtctct ggtttgaatc gtcttaccga atgaatacga
     1681 aatatctata atattttcaa tttcgagttc ggcagttttg tcttgagcaa tgtcataaat
     1741 gactgcatgc caatgtccat gtttacacaa aaggccaata aatataacgt tttcggcttt
     1801 aatattatca tcaaccctat gatctactaa catcacattt cgttctgtac aataaattaa
     1861 aatgtcggaa aacaacattg ggagtgtcat atgttgtgtg tcatcaaatt taatataaaa
     1921 gtccatgcgc ttaagcaact ttcttaagcg tgtttgtgga agacttaaac attgttttat
     1981 aattgcgttg atttcgcttt tatatggtaa tgtcgagtac gactgacttt cattaagcac
     2041 taaaaataag gccgataatt gtgtttcaga taagtttaat gcaattttag attgtgcttg
     2101 ttgtatttgg taaccaccat ttttcccata atgcgcataa attttaacac cttgattttc
     2161 taaatcatca atatctctta aaattgtgcg tttggatacg ttgcaatatt tagctaattc
     2221 taaagcggtc atttttttat tttgttgaat tgcagtaatt attaaatttt gcctttcagc
     2281 tttattcatg acagccacct cctaaaagat gatatattac acaaagtata acataagagt
     2341 gacaatgagg acattttcaa tatcttttta aaaaaatcgg aaaaattttg tggaaaacgc
     2401 ttacatttta aaaaagattg tgctatgata ttacttgtaa cagggggagc gattaaacaa
     2461 aggggtagag ctatataaca gatagctttt aatcgttcaa gttacatcac aacatcttca
     2521 atatttatta cttactttcc tttctatttg tcggctagca cgtgactagc cgattttttt
     2581 atgcataaaa tgaagaagtc ttcagttgta ttgttgattc ttttaatata gggtaataat
     2641 aaaatgataa tgatatgaga agtggtggac gaaatgacag ataaaacatt acagcaaata
     2701 gataagttaa tatgttcgtg gttgaaacaa atagacaatg ttattcctca attaattatg
     2761 gagatgacta cagaaactaa gcgtcatcga tttgatttag ttacaaatgt agataaacag
     2821 attcaacaac aatttcaaca atttttagca acacattttc aggaacacca actattagcg
     2881 gaagaaaaaa gtaatgaaat gattacgaat gaaattaatc atttatggat tatggatccc
     2941 attgatggaa ctgccaattt agtaaaacag caagaagatt attgtattat attggcgtat
     3001 ttttatgaag gtaaaccaat gttatcgtac gtatatgatt atccgcataa aaagctttat
     3061 aaagcaatac gaggagaagg tgctttttgt aatggaatta agatggaaga accaccatcg
     3121 ttaaaattgg aagacgctat tatatcgttt aatgcacaag tgatgaatct ggatacggtg
     3181 caagatttat ttgacgcctc atttagttat cgtttagttg gtgcttgcgg attagattct
     3241 atgcgagtgg caaaaggtca atttggggca catattaata caaatcctaa accttgggac
     3301 attgctgcac agtttttatt tgcagaacta ttgaatctaa aaatgacaac tttagatggt
     3361 aaggcaattg atcatttaaa gggtgcacct tttattataa gtaataaagc atgtcatgaa
     3421 acggtactta aaattttaaa tgcaaatggt ggttatcaaa aatacagata gatattctcg
     3481 agagataaaa aatgataaaa aatagttata cgtctacaga agtatagtaa aaaaattatt
     3541 ttactatata ataaatagag aaaacagaca aagtatgtaa ggaatagtaa aggagtggtg
     3601 ggattgaaac gttcgtctaa aagtaaaatg agcctaccga aaaaaatatt tttatgggta
     3661 ttcggtattt tagtaatctt agcgattgta gctgttgtgt atgtagctgc taaaattttt
     3721 attactggta ataagataca caatccgtta gatcgcaatc attcagaatt aagagataaa
     3781 aaagtgagtt taaacgatgg tgatccattc acaatcgctt tatttggtgt cgattcagat
     3841 gctgatcgta agaaaaaggg tggcggagag cgtagtgata gtattatgat tttatctatc
     3901 aaccctaaaa cgaagaaaac tgaaatagta agtataccgc gtgatacaag agcggaaatt
     3961 gttggccgtg atactactga aaaaattgca catgcctatg catatggtgg accaaatatg
     4021 gctgttaaat cacttgaaaa attaatgaat gtgccaattg atcattatgc gacaatcgat
     4081 atggatggtt tgcataatat gatagatagc atcggcggtg ttgatgtagt aagtaatgat
     4141 acattcactg ttgatggtgt tcgttttaca aaaggacagc aaacacatgt aaatggtgat
     4201 caagcattga agtttattag aagtcgtaaa gaagaaggtg ctggtggtga ctttggtcga
     4261 caacaacgtc agcaaattgt acttgaagcg atggctaata agattgcaag cccatcatcg
     4321 attacacatt ttaatagtct aatgaatgaa attcaaaata atgttaaaac agatttaaca
     4381 ttaggtgatt tgaatacgat tagaagcaat tataaagatg ctaatgacac gattaataaa
     4441 catcaattga gtggtcaagg cggcattcaa agtgacggat tgtactactt cattcctagt
     4501 gagcagtcaa aagctgaaag tactaagtta ttaaaagata atctagagta gtaaactaag
     4561 ggaaaataag ttatcaaaat tgtacacaaa ttaattagat agaatgatct gtaaaaagaa
     4621 tgacgaatag aactaatagc ccctaaatag aaggtgataa cactttgtat ttatgggcta
     4681 ttttatttaa tgcctgattt gatttttata aattaatcca ttacaaattt aatttaaaga
     4741 taaatcttac cttctaaatg atagtagata tttatcaaac ttgggttatt aattttcaga
     4801 aaaagggtat ttataaatag tataacgatt tttaggtgag gtgattatta agtattgtca
     4861 ttaataaggt tgtgaaagat atggcaaaaa gcgtgaaaca gataattgga aaactaataa
     4921 tgataattat tttcaattaa acaaaaagat gatttaattt cgcgcaaaat atttcaaata
     4981 aaaatgattg caaaaataaa aaatacaaaa atacgcttaa cattatacac ttaaagtaat
     5041 acaattatat tgattctatc tgaaagatgt gtggggcatc tttattttag gtggaaatta
     5101 ggcaatttat taaaagtcat ttacggaaaa atatatagat ggggtgaata gtatgcaaga
     5161 acatttggtg gtcacgcttg atggtaaaga ttatcttgta gaaccaggta caaatttact
     5221 tgaattcatt aaatcgcaag atacttttgt gccttcaatt tgttataacg aatcaatggg
     5281 accgatacaa acgtgtgata catgtacagt tgagattgat ggtaaaattg aacgttcatg
     5341 tagtacagtg attgatcgtc caatgacagt aaatacagtg aataatgacg tgaaagatgc
     5401 tcaaaaagaa gcgcttgatc gaattttaga aaagcatatg ctgtattgta cagtatgtga
     5461 ttataataat ggtgattgtg aaattcataa tacgatggat gcatgggggc ttcagcatca
     5521 aacgtatgaa tataaagaga agccatatga aaaagattat ggcccatttt atcgctatga
     5581 tccaaatcaa tgtatcttat gtggccgttg tgtagaagcg tgtcaagata ttgaagtaaa
     5641 tgaaacaatt agaattgatt gggatcgtga gcatccacgt gtcatttggg ataatgatgt
     5701 accgattaat gaatcgtcat gtgtatcttg tggtcaatgc gcaactgtat gcccatgtaa
     5761 tgcgatgatg gaagttaaca tggaaggtaa tgcaggttat atgactgata cggaacctgg
     5821 ttcattagcg gcaatgattg atttaactaa aaaagcagaa cctggttatg gtccactatt
     5881 tgcaatctca gattcagaag cagaaatgcg taaagagcgt attaagaaaa ctaaaacagt
     5941 gtgtacatat tgtggtgtgg gctgttcatt tgaagtttgg actaaagata gagaaatttt
     6001 aaaagtacaa ccatcacatg attctccagc aaataaaatt gcaacttgtg ttaaaggtaa
     6061 gttttcatgg ggacatatta attcagatca acgcttaacg aaaccattag taaggaaaaa
     6121 tggtgagttc catgaagtag aatgggatga agcattaaat gtcattgcag ataattttac
     6181 atctattaaa gaaaagcatg gtccagatgc attatcattc atttcttctt ctaaagcgac
     6241 gaatgaagaa tcgtatttaa tgcaaaaatt agcaagacaa gttattggca caaataacgt
     6301 tgataactgt tcaagatatt gccaagcacc tgcaacaaaa ggcttattta gaacggttgg
     6361 acacggcggt gactcaggta gtattgaaga tttagaaaaa gcagcaatgt ctgtattgat
     6421 tggtacgaat acagccgaag cacatccggt tattgcatca cgcatgaagc gtgctcaaaa
     6481 attatttgga caaaaaatac atgtgtttga tattagaaaa catgaaatgg ctgaacgcgc
     6541 agatcgtttt tatcaaccga aaccaggtac agatttggct tggttaagcg cagtgacgaa
     6601 atatatcatt gatcatgact tacacgataa agcctttatc gaagagtggg ttgaagattt
     6661 tgatgaatat tacaagtcat tagaaacatt tacaatggca ttcgctgaag aggctactgg
     6721 tattccagaa gcagagttga ttaagttcgc tgaagaatgt gccaaagcag agtccgttgt
     6781 catttgttgg gcaatgggta ttacacaaca agacattgga agtgattcaa gtacagcaat
     6841 ttcaaactta ttattagtta ctggaaatta tcgtcgacct ggtacaggcg cgtatccatt
     6901 acgtggacat aataatgttc aaggttgtag tgatatgggt agtatgcctg ataaaattac
     6961 tggctatcaa agtattgaag cggacgatat tcgcgctaaa ttcgaaaaag aatatggcgt
     7021 taaattgaat ccaaaagctg gtaaagataa tcatgaaatg gttgaaggta tccatgatgg
     7081 gcaaattcac tcattatact tatatggaga agatactggt attgtagatt caaatattaa
     7141 ctttgtacaa gctgcttttg aaaaattaga tttcatggta gtccaagatg aatttttaac
     7201 atttacagct acatttgcag atgttgtctt accagcaagt ccttctcttg aaaaagatgg
     7261 tacttttacg aatactgagc gacgtattca acgtttatac caagcattga aaccattagg
     7321 tgagtctaaa cctgactgga aaatattcca agcaattgct aataaattag gcttcgattg
     7381 gaattacaag catccaagcg aaattatgga tgaaattgct agactaacac cattatatgc
     7441 tggcgtaagc tacgaacgtt tagaaggatt taatagtttg caatggcctg tgcatccaga
     7501 tggtacagat gaaccaatac tttatttaga aggatttaat ttcgataatg gtaaagcaaa
     7561 attattccca ttatcatttg ataattactt taagcaagat gaagtttatg atattcatgt
     7621 aaacaatggt cgattgttag agcacttcca cgaaggtaac atgacatatc aaacaccaat
     7681 gcttaaatac aaagtgccac gtgcatttgt tgaaatttct ccagaacttg ctgaagatag
     7741 aggcattcat gaaggtgcag aagttaagtt gatatctgaa acaggagaag cggtattaca
     7801 agttcacgtg acagatcgtg ttaaaggtaa agaaatctat attccattaa ataatgatgc
     7861 gatggaaaat ggagatctag gcgcaattaa cttattaact aatagtgatg ttgatcaata
     7921 cacggataca ccatcttata aacgtacaag ctgtcgtttg gaggtaatta cgaaacgtgg
     7981 taagtcaccg ttgaacccta ataatttccg tgtcaataaa aaacgtcatc cgcagtacag
     8041 tgttcaagta cagaaaaaat gggaacgtcc agattatgtt ttcccaggaa atcaggtgga
     8101 taaataatgg ctgagagaat atcttctaaa attagacgct tagaaaaatc agaagaacaa
     8161 attaagttag aaagtttaaa tgaagttaca gaggcaattg cagcgaataa agatagtata
     8221 ttaaaagcaa tcaaacttat taaagtgtta gatgatgcta aaatattaga tgctttaaat
     8281 ggcgcagtta gaggacgtca agtgattatt aataaatttg ctgttgagct caacaaagat
     8341 atctatacag gactattatc taatatggca tcaatggtat ttttattagg tgaattaaat
     8401 gtatcagatt taagtgactt ccttaataaa gttaataaag gattacatgt tgcaaatcaa
     8461 gcgagtccta atgcgaagac atctgtaaga agtctgtttg gcgttttaaa agatgacgac
     8521 atgaaccgta gtttaacata tatgcttaat atgttaaaag gtatgtctcg agaagattaa
     8581 tatgagtcta taataaatcg gattgatgta aaagtttttt acttttcatc tttccgattt
     8641 tttgtttttg aatataacag tgtttctgca tgtcggtgta ggggcactgt tttaagttat
     8701 attgaaatat tattttgtat ccgagtcttc atcaaacttc aaacaatatt tttgagaatc
     8761 gagaaaatca tgtgctattg ataatcaacc atcgacaggg ctatgaaaca tcatgtatta
     8821 ttatgattat gtaagttatt taaataaaaa gggtgagtgc atattaattc gagaatgtta
     8881 aaaacgtcag ctgtaatttc taatacatta ttagtcatag gtttagtctg tctatttatg
     8941 atgaaattag tgttagctat tacttttttt gctgtttcat tatcaattag tctagttgtc
     9001 tttaatatga tgttcagaga tagaacaacg atgaaagtaa tcgttaacgc ttctttttta
     9061 atcgtaatat tagcgattgt agtggcatat tttttattgt ctaagtaaga aaaatgaggt
     9121 tttgtctata tgaagaagaa tttcaagttg cgtatttcaa cgctactatt gatagttatt
     9181 ttagttgttt tttctgtatt gctcatcgtg aatgaaacta aattgtttaa aaatgatgtg
     9241 aattactctt ttgatgaggc tgtttcaatg caacaaggga aaggtattgt acagacaaaa
     9301 gaagaggatg gtaaatttgt tgaagcaaat aataatgaaa ttgctaaagc aatgactatt
     9361 tcacataaag acaatgatat gaagtatatg gatatcacag aaaaagtgcc aatgtcggaa
     9421 tctgaagtta atcaattgct aaaaggtaag gggattttag aaaatcgagg gaaagttttt
     9481 ctagaagctc aagaaaaata tgaggttaat gtcatttatc ttgttagcca tgcattagta
     9541 gaaacaggta acggcaaatc agaattagca aaaggcatta aagatggaaa aaaacgctat
     9601 tacaactttt ttggtatagg agcattcgat agtagtgctg ttcgtagtgg gaaaagttat
     9661 gctgaaaagg aacaatggac atcaccagat aaggcgattc ttggtggtgc aaagttcatt
     9721 cgtaatgaat attttgaaaa caatcaactg aatttatatc aaatgcgatg gaatccagaa
     9781 aatcctgcgc aacatcaata tgcgagtgac attcgctggg cagataaaat tgccaaatta
     9841 atggataaat cctataagca gtttggtata aagaaagatg atattagaca aacatattat
     9901 aaataagaca tcggtgctta aaggagctgg aacaatttat tgtttcgagc tcctttagcg
     9961 cattctgagt gtgttagtta aaaggatttt aacctaacat aaaacgcggg agtgggacag
    10021 aaatgatatt tttacaaaat ttatttcgtt gtcccacccc aacttgcaca ttattgtaag
    10081 ctgactttcc gccagcttct atgttggggc cccgccaact tgcacattat tgtaagctga
    10141 cttttcgtca gcttctgtgt tggggcccac accccaactc gcattgcctg tagaatttct
    10201 ttttgaaatt ctctgtgttg gggcccctga ctagagttga aaaaagcttg ttgcaagcgc
    10261 attttcattc agtcaactac tgccaatata atattataga ccctaggaca ttgatttatg
    10321 tcccaagctc ccacatcatt gttacaaatg tacattatgt gaatgaatat tgctgtctaa
    10381 atatgcatgt aatatacaat ggtgcagata atacacttaa gtctttaaaa atgaaacgtt
    10441 agttccaaga gtcattttta aacaatagtg catgtgataa aatagaaaag aatgaaaaat
    10501 atagaggtga caatatgaag atagcaatta taggtgcagg catcggtgga ttaacagctg
    10561 ctgcactatt acaagaacaa ggtcatacta ttaaagtctt tgaaaaaaat gagtcagtta
    10621 aagaaattgg cgctgggatt ggtatcggag ataatgtgct taaaaaatta ggtaatcatg
    10681 acttagctaa aggtattaaa aatgctgggc aaatcttatc tacaatgaca gtgttagatg
    10741 acaaagatcg cccgttaact actgttaaat taaaaagtaa tacattgaat gtgacgttac
    10801 cacgccaaac attaattgac attattaaat cttatgtaaa agatgatgca atatttacaa
    10861 atcatgaagt cacgcatata gataatgaga cagataaagt taccatacat ttcgcggaac
    10921 aagagagtga agcatttgat ttatgtattg gtgctgatgg aattcattct aaagtgagac
    10981 aatctgtaaa tgctgacagt aaagtattat atcaagggta tacatgcttt agaggtttaa
    11041 ttgatgatat tgatttaaaa catccggatt gtgcaaaaga atactgggga agaaaaggaa
    11101 gagtaggtat tgttccgtta ttaaataatc aagcatattg gttcattaca attaactcga
    11161 aggaaaacaa tcataaatat agttcgtttg gtaaacctca tttgcaggca tactttaatc
    11221 actatccaaa tgaagttaga gagatcttag acaaacaaag tgaaacaggt atcttattgc
    11281 ataatattta tgatttgaaa ccactcaaat cttttgttta tggtcgtact attttactag
    11341 gagatgcagc acatgcgaca acgcctaata tggggcaagg tgctggacaa gcaatggaag
    11401 atgctatcgt attagtaaat tgttttaatg catacgactt tgaaaaagca ttacagcgtt
    11461 atgataaaat acgtgtcaaa catactgcaa aagtaattaa acgttctaga aaaatcggta
    11521 aaattgccca atatcgtagt cgtttagttg ttgcagttag aaatcgtatt atgaaaatga
    11581 tgccaaatgc attagcagct ggacagacta aattcttata taaatcgaaa gaaaagtaat
    11641 acaacaatat gaaaaccccc gtatgttgaa atgagagctc aacatatggg gggtcttgtt
    11701 tttataatgt cattataata aattcaatta ttagttaacg acaaattgtg gtttctcacc
    11761 ttgaacggca ctaattgcag cattagcaac aattttagac atcatgtcac gtgcttcaaa
    11821 tgtagcatta ccaatatgcg gtgttaatac tacattatta agtgatttta agtcatcggt
    11881 aatgtctggt tcgaattcat atacatcaag tgcagcacct tcaatttcat tatttttcaa
    11941 tgcttgcact agtgcttgtt cgtgcacgat tggaccacga gaggcattga ttaaatacgc
    12001 cgtagatttc atcattttaa attgttctgt atcaattaaa tgatgcattt taggattgta
    12061 agcagcgttg atagtgataa aatctgcatt ctttaatagt gtatctaaat ctacatattt
    12121 tgcaccgatt tctcgttctt tttcttcttt gcgattaggt cctgtgtata gtacatccat
    12181 gtcaaatgct cttgcacgac gcgctactgc actaccaatt tcacctaaac cgataatgcc
    12241 gattgttttc ccagatactt ctctacctct gaaaaataaa ggtgcccatc cgtcaaatcc
    12301 agttgtacgt gataattggt cgccttcaac aatacgacgc gctactgcaa gtactaatcc
    12361 aattgttaaa tcagcagtcg cgtttgttga tgctttaggt gtgtttgtaa catctatact
    12421 tttttctcga gcatactcga tatcaatatt attaaaacca gcaccatagt tggcaatgat
    12481 ttttaagtct ttaccagcat cgataacatc tttatcaacg tttgtagata ataaactaat
    12541 taaggcagtc gcatctttaa cacctttaat taaagtgtct ttatcgacta atcctttacc
    12601 ttcatacatt tcaacttcaa aatgttcttg taaaagtttt aaacctactt ctggtattgc
    12661 accagcaaca taaacttttt ccataaaaga tcactccttt tatcttagta tagtagaaga
    12721 ttagacagta tacaactatg tcataatgtc ttgtgtatca atgatgtaag cgtgtacttt
    12781 tgatggaggt gatataactt aggcactgta ggactatgaa tattgtaatg tggaaaactg
    12841 gatcaattaa attagataac gtagttttaa agttaatagt attgaaaaaa attaatatta
    12901 tgaatatggg agaaaaaaat atatataagt gggtggcaac gaaaaatagc aaaaaaagag
    12961 cttctcctat aaaggaaagc tcaaagtttt tgatgacata tgtactagaa ttaagtttca
    13021 agacaatatg tatcatcgtg tttatattaa atatggatgt agttgtagtt acctgcttca
    13081 cttgcagaaa tagttcttga gcttactgag aaaggtccgc cactataatt catttctgaa
    13141 attgtaactg aaccatcact gtttacactt tctacatatg caacgtgacc aaatggtcct
    13201 tcagaagatt gtaggatagc gcctttagaa ggtgtatggt ttactgtgaa tccagcacct
    13261 tgtgcagcag cagcccaatt attagcattt ccccaagtag atccgatttc tccgcctact
    13321 ttatcatata cataccaagt acattgtcct gcagtgtata agttaccaga atgtgaaatt
    13381 gatgatgtag ttgtcgtagt tgttgtagtc gttgtagttt gagtcgtatt atagttatag
    13441 ttgttataat ttgtataatt ttcagcagcg tctgcatgtt gtgcatgacc tactaatgct
    13501 gtaccaattc ctgctgttaa cgtagttgct gttactaatt ttttcatgaa taaagtcctc
    13561 caaagttcta tatctttttt tataaataaa acgtagcgac tgttttattc tcacatctcg
    13621 aattgatgac aatagttact ttaacaaaat taatgcttct tgtggggaat gttattgatt
    13681 tgtaaaagaa taaaaaaact ttgactaatt ttgtaataaa aattagtcaa agttacagtg
    13741 agattaacag ataattaata ggaaatattt atttgtaata tgtttaaatg aatcgaattg
    13801 ttaaaggtat tacatattct tggccattat aatatttgac acacgcaata attgtgaata
    13861 caaaagataa tattgagaaa gcgaatatgg ataaaatacc gataaacgtg atgatgaaac
    13921 ctataatgat aatgaaatca atatcggtag caattaggaa aacgcctata aaagtgataa
    13981 cgactaaaac gatagaccaa ataatataag aaatcgtata gttaagataa ttttttccag
    14041 cacgatcaac tagtttcgat tcatcttttt tcaataacca tataatcagt ggaccaataa
    14101 tagatgtgaa taaacttaat aaatagataa gcatcgccat aatgttctca tcattggatt
    14161 tgcgattcgg ttgatgattt gttacgtcgt tcatttcagt tgtcatatta gacactcctt
    14221 tgaaaattgt aatattatct ttaactataa caaaatataa tcaaaaataa acatgtttat
    14281 taaacaatta ttaaaaataa aaataattgg tggacgtcgg cgtttaaata ggttaattta
    14341 aggttatata tacttaacat ttataatgat gcgtaatgaa ttcgcatcat ttttatattg
    14401 tcttacgtat aatttgtttt taattttaac caaagataga aagagggttg tttatgaaaa
    14461 tagcaattgt aggatcagga aatggcgcag ttacggcagc agtagatatg gtgagcaaag
    14521 gccacgatgt taaattatat tgtcgtaatc aatctataag taagtttcaa aacgcaatcg
    14581 aaaagggcgg atttgatttt aataatgaag gtgaagaacg tttcgtaaaa ttcactgata
    14641 ttagtgatga tatggaatat gttttaaaag atgctgaaat tgttcaagtg attattccat
    14701 cttcatacat agagtattat gctgatgtaa tggcagagca tgtaactgat aatcagttga
    14761 tattcttcaa catggctgca gcaatggggt caattcgttt tatgaatgtt ttagaagata
    14821 gacatattga aacaaaacca caattagcag aagctaatac gttgacgtat ggtacgcgtg
    14881 tcgattttga aaatgcagca gttgatttat ctctaaatgt acgtcgtatc ttcttttcaa
    14941 catatgatag aagctgtcta aatgattgtt atgacaaagt ttcaagtatt tatgatcatt
    15001 tagtaaaaga ggaaagctta attaaaacaa atcttgaaaa tggtaatcct gaagtgcatc
    15061 cagggccaac attattgaat gtcggtcgta ttgattatgc tggtgagttc gctttatata
    15121 aagaaggaat tactaaacat acagttagat tacttcatgc aatcgaatta gaacgtttaa
    15181 atttaggccg tagattaggt tttgaattat caacagctaa agaatcacgt attgaacgtg
    15241 gttatttaga acgtgataaa gaagatgaac cattaaatcg tttgtttaat acaagcccag
    15301 tattttcaca aattccagga ccaaatcatg tagaaagcag atatttaact gaggatattg
    15361 catatggttt agtattatgg tcaagcttag gtcgtgttat tgatgtaccg acaccaaata
    15421 tagatgcagt aattgtcatt gcatcgacaa tcttagaaag agatttcttt gaagaagggt
    15481 taacagttga atccattggc ttagataagc ttgatttaga aaaatattta aaataaatga
    15541 tggcttgaag atagaaaagg atatagcatt atgcaaaagc aataaattga agaaaagagg
    15601 tttctcatca ataagcgaag gggacgatag atgatgacaa gaaaaccaac ctttttagaa
    15661 tcaatttcga caatgattgt aatggttatt gttgttgtaa caggctttgt gttttttgat
    15721 attccaattc aagtattatt aattattgcc tcagcatatg ccgcatggat tgcaaaacgt
    15781 gtaggcttaa catggcaaga tttagaaaaa ggcattgcag aacgtttaaa tactgcaatg
    15841 cctgcaattt taataatact agcggtagga attatagtag gcagttggat gttttctggc
    15901 acagtgccag ccttgattta ttatggctta gatttattga atccaagcta ttttttaata
    15961 tcagcctttt ttataagtgc tgttacatct gtagcaactg gtacagcatg gggctctgca
    16021 tcaactgcag ggattgcact tatttctatc ggtaatcaat taagtatctc accaggaatg
    16081 gctgcgggtg ctattatagc aggcgctgta tttggcgata aaatgtcacc tttatcagat
    16141 acaactaatt tagcggcgct tgtcactaaa gtgaatatat ttaaacacat tcgttcaatg
    16201 atgtggacaa caatacctgc gtcaatcatc ggtttattag tatggtttat tgctggattt
    16261 caatttaaag ggcattcaaa tgataagcag attcaaacat tgttatcaga gcttgcacat
    16321 atttatcaaa ttaacatatg ggtctgggtt cccttaattg tgatcattgt ttgtttgcta
    16381 tttaaaatgg ctacagtgcc agctatgcta atatcaagct tttctgccat tatagtggga
    16441 acttttaatc atcatttcaa aatgacagat ggtttcaaag caatatttag tggttttaac
    16501 gactcaatga tacatcagtc tcatatttca tccagtgtga aaagcttgtt agaacagggt
    16561 ggtatgatga gtatgaccca aatattagta acgatatttt gcgggtatgc atttgcaggt
    16621 attgtagaaa aagcaggatg tttagaagtc ttattaacta ctatttctaa aggcatccat
    16681 tctgtaggaa gtttaatatg tattactgtt atttgttgta ttgcgcttgt attcgctgca
    16741 ggtgttgctt cgattgtaat tataatggtc ggtgtgttaa tgaaagattt gttcgaaaaa
    16801 taccaagtct ctcgtttcgt tttatcaaga acacttgaag actcaagtac gatggtctta
    16861 cctttaatac catggggaac atcgggtatt tactatacga atcaacttca tgtctctgtt
    16921 ggagaatttt tcatatggac agtaccatgt tatttatgcg caattatagc aattatctat
    16981 ggttttacag ggataggtat taaaaagtca tcgaattcac gtttaactta atgtgagcgt
    17041 gga
//
LOCUS       DAPIDM010000045        17026 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000045 DAPIDM010000000
VERSION     DAPIDM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.045"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            496..1677
                     /locus_tag="RJE42_001301"
     CDS             496..1677
                     /locus_tag="RJE42_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV5876730.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEINEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPAIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     SEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(1760..2512)
                     /locus_tag="RJE42_001302"
     CDS             complement(1760..2512)
                     /locus_tag="RJE42_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDV5876731.1"
                     /translation="MTHYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(2555..4783)
                     /gene="metE"
                     /locus_tag="RJE42_001303"
     CDS             complement(2555..4783)
                     /gene="metE"
                     /locus_tag="RJE42_001303"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV5876732.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKALIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     SSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(4780..6621)
                     /locus_tag="RJE42_001304"
     CDS             complement(4780..6621)
                     /locus_tag="RJE42_001304"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDV5876733.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TNRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(6590..7750)
                     /locus_tag="RJE42_001305"
     CDS             complement(6590..7750)
                     /locus_tag="RJE42_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDV5876734.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHKLLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTAAHLEQAKNGGAVIGLRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     ATLPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(7747..8850)
                     /locus_tag="RJE42_001306"
     CDS             complement(7747..8850)
                     /locus_tag="RJE42_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV5876735.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAEKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(8916..9285)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9514..10359
                     /locus_tag="RJE42_001307"
     CDS             9514..10359
                     /locus_tag="RJE42_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV5876736.1"
                     /translation="MSELSKSEDQRITKTKDEQIKKIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQDDFVRIIEQLN
                     RKYGK"
     gene            10513..11394
                     /locus_tag="RJE42_001308"
     CDS             10513..11394
                     /locus_tag="RJE42_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDV5876737.1"
                     /translation="MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            11424..11627
                     /locus_tag="RJE42_001309"
     CDS             11424..11627
                     /locus_tag="RJE42_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDV5876738.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            11639..12736
                     /gene="ychF"
                     /locus_tag="RJE42_001310"
     CDS             11639..12736
                     /gene="ychF"
                     /locus_tag="RJE42_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV5876739.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYFMQDGDIVHFRFNV"
     gene            complement(12822..13013)
                     /locus_tag="RJE42_001311"
     CDS             complement(12822..13013)
                     /locus_tag="RJE42_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876740.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYNGEG
                     KQFFSTQDIINASKIIPFF"
     gene            13257..13553
                     /locus_tag="RJE42_001312"
     CDS             13257..13553
                     /locus_tag="RJE42_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV5876741.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            13574..14077
                     /gene="ssb"
                     /locus_tag="RJE42_001313"
     CDS             13574..14077
                     /gene="ssb"
                     /locus_tag="RJE42_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV5876742.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            14129..14371
                     /gene="rpsR"
                     /locus_tag="RJE42_001314"
     CDS             14129..14371
                     /gene="rpsR"
                     /locus_tag="RJE42_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV5876743.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            15183..15497
                     /locus_tag="RJE42_001315"
                     /pseudo
     CDS             15183..15497
                     /locus_tag="RJE42_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            15863..16231
                     /locus_tag="RJE42_001316"
     CDS             15863..16231
                     /locus_tag="RJE42_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDV5876744.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLIYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            16411..16983
                     /locus_tag="RJE42_001317"
     CDS             16411..16983
                     /locus_tag="RJE42_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDV5876745.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDANTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDQ"
BASE COUNT         5736 a         2620 c         3178 g         5492 t
ORIGIN      
        1 tactatttat tttttctagt gaataggggc aaatttggcg atgaagtgga aggagaggtg
       61 actgcaaggt aattgcggaa ttaacaatca tcagcgattt aatatttgac tagagacgta
      121 atggtaagaa aaagttgatg agaaattgat ggtgaaacca gatgtgaata gcgctgcaat
      181 gatagatagc atttaattag agtcataacg cgaaatgatt aatgataatt tgtggtaaat
      241 gaaagcataa ttttgtacta tagatgagga tgatagagca tatttaagag tgtgaaatgt
      301 taaagtgaaa ccgtttacgt ttccgattgc ccaaacaaat tacatcattg tataatatga
      361 tttgttaaat gcataacaag aatgaaaatg taacatacgt agcaattggt ttcataaatt
      421 ggatgttagt ggcgtattgg ttcattagac gtattagtaa taaaattgta tatatcataa
      481 ggagatgaat atgacatgac gagagtcgta ttagcagcag catacaggac acctattggc
      541 gtttttggag gtgcgtttaa agacgtgcca gcctatgatt taggtgcgac tttaatagaa
      601 catattatta aagagacggg tttgaatcca agtgagatta atgaagtcat catcggtaac
      661 gtactacaag caggacaagg acaaaatcca gcacgaattg ctgctatgaa aggtggcttg
      721 ccagaaacag tacctgcatt tacagtgaat aaagtatgtg gttctgggtt aaagtcgatt
      781 caattagcat atcaatctat tgtgactggt gaaaatgaca tcgtgctagc tggcggtatg
      841 gagaatatgt ctcaatcacc aatgcttgtc aacaacagtc gctttggttt taaaatggga
      901 catcagtcaa tggttgatag catggtatat gatggtttaa cagatgtatt taatcaatat
      961 catatgggta ttactgctga aaatttagta gagcaatatg gtatttcaag agaagaacaa
     1021 gatacatttg ctgtaaactc acaacaaaaa gcagtacgtg cacagcaaaa tggtgaattt
     1081 gatagtgaaa tagttccagt atcgattcct caacgtaaag gtgaaccaat cgttgtcact
     1141 aaggatgaag gtgtacgtga aaatgtatca gttgaaaaat taagtcgctt aagaccagct
     1201 ttcaaaaaag acggtacagt tacagcaggt aatgcatcag gaatcaatga tggtgctgcg
     1261 atgatgttag tcatgtcaga agacaaagct aaagaattaa atatcgaacc attggcagtg
     1321 cttgatggct ttggaagtca tggtgtagat cctgctatta tgggtattgc cccagttggc
     1381 gctgttgaaa aggctttgaa acgtagtaaa aaagaattaa gtgatattga tgtatttgaa
     1441 ttaaatgaag catttgcagc acaatcatta gctgtagatc gcgaattaaa attaccttct
     1501 gaaaaggtga atgttaaagg tggcgctatt gcattaggac accctattgg tgcatctggt
     1561 gctagagttt tagtgacatt attgcatcaa ctgaatgatg aagttgaaac tggtttaaca
     1621 tcattgtgta ttggtggcgg tcaagctatc gctgcagttg tatcaaaata taaataataa
     1681 gaaaacaggt tatcacaaca gtattaatta catgttggca taacctgttt ttatttgttt
     1741 atggatttat tgggtaatat tagtcatttg atggtttaat tgcaaatgct ctaacaggga
     1801 acccaggtgc atcttttggt ttagggctga tagcgtaaat gatggcgcca cgagttggta
     1861 attgatctaa attagttaat aactcgactt ggtatttatc ctgaccaaga atataacgtt
     1921 cgccaactaa atcaccattt tttacaacgt ccacagatgc atcggtatcg aatgtttcat
     1981 gaccaacagc ttcaacacgg cgttcttcaa ttaagtactt caaagcatct aatccccaac
     2041 ccggtgcatg ttgttgtccg ttcgcatctt tgttttcaaa cgtttcaata ttaggccaac
     2101 gttttgacca atcggtacga agtgcaacaa aagtgccagg ttcaatagta ccatgctctt
     2161 tttcccatgc ttctatatgc gcacgtgtta cgatgaaatc attgttgttc gctacttctg
     2221 ttgaaaagtc taatacaatt aacggcaata ccaattcttt taaatcaatg tcttctaaat
     2281 aacgtttgtt ctcgacaaag tggattggtg catcaatgtg agtaccatat tgcgttacaa
     2341 tattccaacg ttgcacatag aaaccatgat ctttaaccgt gaataaagtt gaaacttcac
     2401 ctttttcaaa ctcactaaaa cgtggtattt ctggatcaaa tgtatgcgtt aaatcaaccc
     2461 aagttgcttg ttttaaagta tttaattgtt gccataaagg atagtgtgtc ataaaatcac
     2521 ccgtttttag tttattatat gataaatgct gcgattattc ttggcgttta gctttaacag
     2581 cattcacaag tacagtcaat gcatctttaa cttcttcttc ttttcgcgtt ttcaatccac
     2641 agtcagggtt tacccagaat aatgagcggt cgatttgttg tagtgaacga ttgattgctg
     2701 tagtaatttc ttcttttgtt ggaatacgtg gactatgaat atcatataca cctagaccaa
     2761 tacctaaatc ataattaata tcttcaaagt ctttaattaa atcaccatgg ctacgagatg
     2821 tttcaattga aataacatca gcatctaagt catgaatagc atgaataatt tgaccgaatt
     2881 gagaataaca catatgtgta tggatttgag tttcatcacg aactgaagac gttgcaagtt
     2941 taaatgataa aacagcatct ttaagatatt gttcgtgata ttcagagcgt aatggtaagc
     3001 cttcacgtaa tgcaggttcg tcaacttgga taactttgat tcctgcagct tcaagtgcta
     3061 atacttcttc gttgattgct aaagcaattt gatcttgaac aactttacgt ggtaaatcaa
     3121 cacgttcaaa tgaccaattt agaattgtta caggcccagt taacatacct ttaactggtt
     3181 tatctgttaa gctttgtgca taaactgttt catcaacagt taaaggcgct gtccatttta
     3241 catcaccata aatgattggt ggttttacgg cacgtgaacc atatgattgc acccaaccga
     3301 atttagttac taagaaacct tgtaattttt ctccgaagaa ttcaaccatg tcattacgtt
     3361 caaattcacc gtgaactaat acatctaagc cgatgtcttc ttgaatttta atccatcgag
     3421 caatttcatt ttttaagaat gtttcatatg cttcgtctgt aatgcgtttg tttttccaat
     3481 ctgcacggta ttttcgaact tctcggcttt gtgggaatga tccaatagtt gttgttggta
     3541 aatccggtaa gttcaaacgt ttttgttgtt gttcaatacg ttgcgcgaat ggtgattgtc
     3601 ttgaagtacg cacgctttcg aaatcataat ctaagttttt gaatgattga ttttggaaac
     3661 gctcataacg tgcttttaat ttatcatatt taacactatc gttttgatta aataggcgac
     3721 gcaatgcatc taattcgtct aatttttcag ttgcaaagct taagccttcg ccaacacttg
     3781 aatctaatgt ttcatcatct aaagatactg gaacatgtaa taatgaagat gatggttgaa
     3841 tgacaagctc attagtgtgt gctaacaatt tatcgattaa ggctttttta gcttcaatgt
     3901 cacttgccca tacattacga ccatcaataa ttccagcgta taatgttttt gatttatcaa
     3961 aatctcctgc ttcaatttgt ttaaggttat agccattatc atggacaaag tctaaaccta
     4021 aaccaccaac aggtaaagaa cttaagaatt taagatgtgc acgttcgaag tatgtttgaa
     4081 tcactaattt tttagcaaca ccagcttttt cgaaatagtc ataagcttca cgtgtaatat
     4141 tttcatagct ttcgctgtcg tctgtaacta agattggctc atcaacttga atgtactcag
     4201 cacctgcatc aattaatgat tcaaacactt ctttataaag tggtaataac gttttaactt
     4261 tttcttcaaa agtttggtga ccgccttttg ataatttaac aaaagtaatc ggaccaacga
     4321 tgacagggtg agcgttaacg tttaaagatt gggcatattt aaagcgatct aataatacgt
     4381 tgcgactcac tttaggctca acattgtccc attcaggtac gatgtaatga tagttagtgt
     4441 taaaccattt tataagtgca cttgcaacat ggtctttatt accgcgagca atatcaaata
     4501 ataagtcatc atcgatagtt ctaccttgga aacgttcagg gatgatgttg aataataatg
     4561 acgtatctaa aatatggtca tataaagaga aatcaccaac tgggatgcta tctaagtgat
     4621 agtacttttg taataataaa ttttctttat gtagatcagt taatgtttga tctaattctt
     4681 ctttagaaat tttctttgcc caataacttt cgatggcttt tttccattct ctttttctac
     4741 ctaatcttgg gaatcctaag tttgatgttt taattgttgt cataatattg cctccttgtg
     4801 agcagtaata gattttgagt atgctgcaag ttctaatgaa tcttcgacat tttgaaacgg
     4861 tgtgataatg tataaaccat taaaatattc atgaacagta tcgattaaat cctttgaaag
     4921 cttaagactt agttctcgtg ttttggcttt atcatcttta actgcttcaa attgttgtaa
     4981 aatttcatct gacatcttga ttcctggcac ttcattatgc aaaaagagtg cgtttttgta
     5041 acttgcgata ggcataatgc ctatgaaaaa tggtttgttc aagtgcttag tggcatggta
     5101 aatttcaatg attttctctt tgctgtacac gggttgtgtt ataaaataag acattccgct
     5161 ttctatcttt ttctctaatc gttttacggc accatctaat ttacgaacat tagggttaaa
     5221 ggcgccagcg atgttgaagt gtgtacgttt cttcagcgca tcaccgtcag tgttaatacc
     5281 ttgattaaat cttagagcga gttcagttaa tcctttagaa ttaacatcat agacattggt
     5341 tgcacctggt aagtgaccaa cttttgaagg atcaccagtt atggctaata tttcgttaac
     5401 gccaatgagc gataatccaa gtaaatggga ctgcaagccg attaagtttc ggtctcgaca
     5461 tgtaatatgt acgagtggtt caatgttata atattgttta attaagcttg cagcagcaat
     5521 gttgctaatt ctgacagttg ccaatgaatt atctgcgagt gttaccgcat ctacattagc
     5581 tttgtcaagt ttagcgatat tttcaaaaaa tctattcgtg tctaaatgtt tcggtgtatc
     5641 caattcgata ataacggttg gacgttcttg aaccttagat gttaatgatt gtctaacttt
     5701 attttgagat ggattgaaaa gtgctttcgt tggtatcgga atcacttttt tgtcattaac
     5761 aggtttaagt gtctgaatag attctttaat aaatttgatg tgctcagggg ttgtaccaca
     5821 gcaaccacca attaaacgaa caccttcgcg aattagattt tgagcaactt gaccgaaata
     5881 ttgtgcattg tcactatact taaattcact attttcaata tctaataagc tggcatttgg
     5941 ataacaagat aagtatgcgt gctctggtaa ttcaatatgt gtgaaagact cttgcatatg
     6001 gtgcgggcca tgatgacaat tgagtcccac gatatttgca ccacattgaa cgagttgttt
     6061 taatccttca ttgattgcct gaccattaac taagtaattt gtgtttgaag cggttaattg
     6121 agcaatgatt ggaatgtcgt atttctttct cgttcgtgaa atgacatttg ttagctcttc
     6181 taggtcgtaa tacgtttcga aaagtagcgc gtcaacacct tcttcaatta aggtgtctat
     6241 ttgaatttca gtatgataaa gaatagtttg taagctgata tcctcttgtt tgatacctct
     6301 aaacccacca actgtgccta atatatacgt atctttattt gctgcttttt ttgcgatgcg
     6361 aacggcggct tgatgtattg ctttaacttt atcttcgaga ccgaatcgtt ttaacttttc
     6421 aaaatttgca ccataagtat tggtttgaat gacatcagca ccagcttcga tatatgaacg
     6481 atggatacgc tcaactttat ctggatggct aagattatat gcttctggac aggtgtctaa
     6541 accttcagag tataaaatgg ttccaatagc accatcagct actaaaacat tatctttcaa
     6601 ttgtgtgagg aattgactca ttgaatgcct cctttaatgc gtatttgatg tctgcaatga
     6661 gttcatcagg atcttcgaga ccaacactta atcggaatag accgaaagtg ataccacgtt
     6721 cttgtctcac ttcttcaggt agtgttgcgt gagacattgt tgctgggtgc gaaagtattg
     6781 tttccacacc acctagactc actgaaacga gtggtaatgt tagtgcatcg acaaattgtt
     6841 gtgctttaga ctcatcagct aaacgtaagc caataacggc accgccattt ttagcttgtt
     6901 ctaaatgagc tgcagtgagt cccggataat aaacttctga aatttcatct tgctttatta
     6961 aaaatgacac gattttttgc gcgttttcga cagattgttt aaatctgatt ggaaatgttt
     7021 ttaaatgttt agcaagcgtc cagctatctt gagcagataa catattgcct gtaccatttt
     7081 gtattaaata aagagcttca ctaattgcct cattattagt aatgacagca ccagcaatta
     7141 aatcgctatg tccacttaaa aattttgtag cactatgaat gacaatatca gcgccaagta
     7201 ataaaggtga ttgacctaac ggtgtcataa atgtattgtc cacagctacc agtagcttat
     7261 gcttttcggc tattttagaa acagctttga tatcagtaat tttaaaacag ggattcgatg
     7321 gtgtttcgat ataaattaat tttgtgtttg attgaatggc accttcgatt tgttcgagct
     7381 ttgtagtatc tacggttgta aattcaatat taaaacgatt caaaatttgt tcagtgaggc
     7441 gaaaggtacc gccatataca tcatcaggta agatgacatg atcaccagat ttgaaagtca
     7501 aaagtactgc tgaaatagca gcaatacctg atgcaaaagc aaaagcgaat tttccctgtt
     7561 ctaatcgtgc taacttctct tctaaaagtt cacggttagg gttgccactt cgtgcataat
     7621 catatttaac atcgccacca agacttgttt gatggaatgt tgaagaatca tagagtggtg
     7681 ggttagctga atgatattcc acacctctac gccaatcgaa tatcacttct gtctcttttg
     7741 aaagtgtcat acaatctctc caatctgagc tttatctaat gcttggatga tatcgcgttc
     7801 gatgtcttca taattttcaa cgcctagtga taagcggatt aaatactcat caatgccacg
     7861 tttatctttt tcagcatctg gcatatcaac atgtgtttgt gtgtaaggga aggtcactaa
     7921 tgtttcagtg cctcctaaac tttctgcaaa aatacaaata tctaaatttt ctaataattt
     7981 agcgacgcta taggccttgt taagtcttaa actaagcatg ccagtttgcc cgctatatag
     8041 tacttcgtca attgcttgaa gtgactgaca tttttcagca agttttcttg cgtttgattg
     8101 cgcacgctca atgcgtaaat gcaaagtttt aagtccacgt aacaacaaat aactatctat
     8161 tggtgatagt gttgcgccag tcatgttgtg aaaatcaaac aactgttgcg cgagtgattc
     8221 atctttgacg gttacgacac ctgctagtac atcgttatgt ccaccaatat atttcgtagc
     8281 tgaatgtaag actatatcag caccttctgc taatggtgtt gaaagataag gtgttaaaaa
     8341 agtattgtcg ataattgaca ataagccttt agctttacaa agttgatagt acggctttac
     8401 atcaatagca atcatttgtg ggttagatat aggttcaatg aataatgcaa ctgttttatc
     8461 agtgatttct ttttcaactt gttcataatc tgtaaaatca acgtacttaa atttgatatc
     8521 gtattgttgc tcgtaaaatt caaataatct aaatgtgccg ccgtataaat cgaatgaaac
     8581 taaaatttca tcgtgtggtt taaataaatt acatattaat tgaatggctg acattccact
     8641 tgatgtagcg aatgatgcaa taccatgttc gagtttggca aaacaggttt caaatgttga
     8701 gcgtgtagga tttttagtac gtgtgtaatc aaatcctgtc gattgtccta gttttggatg
     8761 cttgtaggca gtagataaat ggattggatt cgctatagca ccggttgaat catcagttaa
     8821 tgtgatttgg gctaactgtg tatccttcat attaagaccc tcctagtaag aaaaaataaa
     8881 aaagcttccg tccttcgtac ccgaatgaat cggataaaaa ggacgaaagc ttatgtttcg
     8941 cggtaccacc tttatttgtt attccatcgc tgaaataacc ttattcagta cgcattaaaa
     9001 gtaaatatgc ttactgaaca attatcacaa tttaagtcgt taagtaagga tatagtaatg
     9061 tgctatccca tacttattaa caaaaaatcg tgcgtaaaga atccagtacg ccatttaaca
     9121 tcaatgttaa tactgtatcg ctataacggg cgaacccgta gacacctcat attggcatca
     9181 acactccaag gccattttca aacacgcttt caaaatcttc tctcagctac taaagactct
     9241 ctgtataagc agggtgtgtt ttactttcct ctttattgtg tttacgtttc attaaactgt
     9301 tataagatat taattagctt acagagtaaa aaaagatttg tcaacaatta ttcagaaaat
     9361 tttgatttaa aagttaattt gtttgtgaaa ttgtaattgg tatcttgaag ttgaaaaatg
     9421 aattattttt taaataaagt gtggttaatg gttgtctgac tcatttagaa tacataaaat
     9481 atatttaact gttgttatca aataaaaagt gatgtgagtg aattgtcaaa aagtgaagat
     9541 caacgtatta ctaaaacaaa agatgaacaa attaagaaaa tagatatatc ggatatcaaa
     9601 ccgaatccgt atcagccccg aaaaactttc gatgaaaatc atttaaatga tttggcagat
     9661 tcaattaagc aatatggtat tttgcaacca attgtgctaa gaaaaacagt tcaaggttat
     9721 tacattgtag ttggtgaaag aaggtttaga gcttcgaaaa ttgctggtct aaaatacgta
     9781 tcagcgatta tcaaagattt aacagatgaa gatatgatgg aactggcggt catcgaaaat
     9841 ttacaacgag aagacttaaa tgcgattgaa gaagctgaaa gttatcaacg tttgatgaca
     9901 gatttgaaaa ttacacaaca agaagtagcg aagcgattga gtaagtcgcg cccgtatata
     9961 gcgaatatgt tgaggttatt acatttaccg aaaaagattg ctgacatggt aaaagatggg
    10021 cgactgacaa gtgcacatgg acgaacgtta ttggcaatta aagatgaaca acaaatgctt
    10081 aggttagcga aacgggttgt taaagaaaag tggagtgtta gatatttaga aaaccatgtt
    10141 aatgaattaa aaaatgtttc gtcaaagtcg gaaacagaca aggtagatat aactaagcct
    10201 aaatttataa agcaacaaga acgacagttg cgagaacagt atggcgcaaa agtagatata
    10261 tcgataaaaa aatcagtcgg caaaatctca tttgagtttg aatcacaaga tgactttgtg
    10321 agaatcattg aacaattaaa tcgtaagtat ggtaaatagt tacacaattt tatataatat
    10381 cactttgtga atctatagat atatcgatag ttatctatat tctttgaaga tgatgcgatt
    10441 tcagtaggtt ggaagaatac aacaatatta ataaaattat ttgagactat atagaaggga
    10501 gtaacgctaa ttatggatca agttatgagt attatttcat cgctattcga accattaact
    10561 aaaattgaaa catatgaaaa tattgcaact aaaatcgcta tgattgttat ttatattatc
    10621 gtagccctca tagttattaa aatactgaat aaaatgattg aacagggatt taagattcaa
    10681 aataaaagta aaaagagtaa caaaaagcgc tctaaaactt taatatctct tgttcaaaat
    10741 gtagtgaagt atatcgtttg gtttatagtt attacgacga ttttaagtaa atttggcatt
    10801 agtgttgaag gtgttattgc aagtgctggt gtcgtaggtt tagcagtagg ttttggtgcg
    10861 cagaccattg ttaaagacgt tattactgga tttttcatta tatttgaaag ccaatttgat
    10921 gtaggtgatt atgttaaaat aaacaacggt ggtacaactg tagcagaggg aacagttaaa
    10981 tcaattggac tccgttcaac acgtattaat acaatatctg gagaacttac aatcttgcca
    11041 aatagtagca tgggtgaaat aacgaattac tcaattacga atggaacagc aattgttaaa
    11101 attccagtat ctgtagatga aaatattgat aatgttgaga agaaattaaa caaattattt
    11161 acatctctgc gtagtaaata ttatttattt gtaagtgatc ctgttgtgat tggtattgat
    11221 gcctttgaag atactagagt aatattaagg gtttcagcag aaacaatacc aggtgaaggt
    11281 ttctctggtg cgcgtattat tcgtaaagaa gtacaaaaaa tgtttttaca agaaggtatc
    11341 aagacaccac aaccaataat gacacctttt aatcatagtg aaaacggtgt ttagtagttt
    11401 ataatacatg gaggtcatat ttaatggcgt caaaatatgg aataaatgat atagtagaaa
    11461 tgaaaaaaca acatgcgtgt ggaaccaatc gttttaaaat tattagaatg ggtgctgaca
    11521 ttagaattaa gtgtgaaaat tgtcaacgaa gcattatgat tccacgtcaa acgtttgata
    11581 aaaaactaaa aaaaatcatc gtatctcagg atgatacaca aagataggag aatgattcat
    11641 ggctttaaca gcaggtatcg ttggcttacc aaacgttggt aaatcaacat tatttaatgc
    11701 aattacaaag gcgggtgcct tggcagcaaa ctatccgttc gcaactatag acccaaacgt
    11761 gggtattgta gaagtgccag atgcaagact acttaaatta gaagaaatgg ttcaacctaa
    11821 aaaaacatta cctactacat ttgaatttac agatattgcc ggaattgtta aaggtgcttc
    11881 taaaggtgaa ggtttaggta ataaattctt atcacatatt agagaagtag atgcaatttg
    11941 tcaggttgtt cgtgcttttg acgatgataa tgtaacacat gtagctggtc gagttgatcc
    12001 aattgatgac atcgaagtta tcaatatgga attagtactt gcagacttag aatcagttga
    12061 aaaacgctta cctagaattg aaaaattggc acgtcaaaaa gataagactg ctgaaatgga
    12121 agtgcgtatt ttaacaacta ttaaagaagc tttagaaaat ggtaaacccg ctcgtagtat
    12181 tgactttaat gaagaagatc aaaaatgggt gaatcaagcg caattactga cttctaaaaa
    12241 aatgctttat atcgctaatg ttggtgaaga tgaaattggt gatgatgata atgataaagt
    12301 aaaagcgatt cgtgaatatg cagcgcaaga agactctgaa gtgattgtta ttagtgcaaa
    12361 aattgaagaa gaaattgcta cattagatga tgaagataaa gaaatgttct tagaagattt
    12421 aggtatcgaa gaaccaggat tagatcgatt aattagaaca acttatgaat tattaggatt
    12481 atcaacatat tttactgcag gtgtgcaaga agtacgtgct tggacattta aacaaggtat
    12541 gactgcacct caatgtgctg gtatcattca tactgatttt gaacgtggat ttatccgtgc
    12601 tgaagtaaca agttatgacg actatgtaca atatggtggc gaaagtggcg ctaaagaagc
    12661 gggcagacaa cgattagaag gtaaagaata ttttatgcaa gatggcgata tcgttcattt
    12721 cagatttaat gtataaacga tagagtgaag gtaattaaat agtatatatg tagaagaggc
    12781 ggaatcaatt gttcgcctct tttaattatg cgtataattt attaaaagaa tggaataatt
    12841 ttactcgcgt taataatatc ttgagtgcta aaaaattgtt tgccttcgcc attataagca
    12901 ggctctaaaa caagattagc ctttgcacaa taaagccatt caggatgaat gccactatta
    12961 agtatctctt ggaattcttg aaaatcttta gaccaatcaa tatttaaatt cattccttac
    13021 cacctcacaa ccattataga acatgcgttc gcttttgtga agtgtatttt taaaaatatc
    13081 atagaaaatt tcaaatgaat taatgtcaaa aatgatatga agagtagttt gaattattat
    13141 tgtaaaaagt aatggcgcat gatataattc tttattgtga gtaatgaaaa ttattccttg
    13201 cttatctgtt ttaagattga taagccgtat agaccacaag gaggtgcaaa tataaaatga
    13261 gaacatatga agttatgtac atcgtacgcc caaacattga ggaagatgct aaaaaagcgt
    13321 tagttgaacg tttcaacggt atcttagcta ctgaaggtgc agaagtttta gaagcaaaag
    13381 actggggtaa acgtcgccta gcatatgaaa tcaatgattt caaagatggc ttctacaaca
    13441 tcgtacgtgt taaatctgat aacaacaaag ctactgacga attccaacgt ctagctaaaa
    13501 tcagtgacga tatcattcgt tacatggtta ttcgtgaaga cgaagacaag taataattag
    13561 agggggcgtt taaatgctaa atagagttgt attagtaggt cgtttaacga aagatccgga
    13621 atacagaacc actccctcag gtgtgagtgt agcgacattc actcttgcag taaatcgtac
    13681 gttcacgaat gctcaagggg agcgcgaagc agattttatt aactgtgttg tttttagaag
    13741 acaagcagat aatgtaaata actatttatc taaaggtagt ttagctggtg tagatggtcg
    13801 cttacaatcc cgtaattatg aaaatcaaga aggtcgtcgt gtgtttgtta ctgaagttgt
    13861 gtgtgatagc gttcaattcc ttgaacctaa aaatgcacaa caaaatggtg gccaacgtca
    13921 acaaaatgaa ttccaagatt acggtcaagg attcggtggt caacaatcag gacaaaacaa
    13981 ttcgtacaat aattcatcaa acacgaaaca atctgataat ccatttgcaa atgcaaacgg
    14041 accgattgat ataagtgatg atgacttacc attctaataa aaattaacga aattaaagcg
    14101 aaaaaattat caaaggaggc acacaatcat ggcaggtgga ccaagaagag gcggacgtcg
    14161 tcgtaaaaaa gtatgctatt tcacagcaaa tggtattaca catatcgact acaaagacac
    14221 tgaattatta aaacgtttta tctcagaacg cggtaaaatt ttaccacgtc gtgtaactgg
    14281 tacttcagct aaatatcaac gtatgttgac tacagctatc aaacgttctc gtcatatggc
    14341 attattacca tatgttaaag aagaacaata atatataatt tattgtcaaa ccccgtaggc
    14401 ataggcttat ggggtgtttt tgtgttttgg gataaaagat gaagggcaaa aaagaggatg
    14461 tgaatgtttt gtgttcggaa tttgcacaaa gatatgttta tattgcaaaa ataatatgaa
    14521 tttagatgca taaaaaaaga ggcaaccgtc agtaacagtt aacctcaagt gcattccgca
    14581 gatatgcacc gctatttatg tataatatta atctgttttt agataaaaat caaggttatt
    14641 gataaagttc tttaaacttc ttaatttagg tcttgctcga acgcctataa ttttatcata
    14701 agtcgactcg cttatctttg atcgtaaaaa atcaaggttt tgttcgaatt gccttataat
    14761 tctttggagt tctgctttag aataagagag accagaacaa attagaatta ataaaatgag
    14821 ataatcaatt aactttaccg tcgatatcta gcgttttatc ttcatagttt atattttctc
    14881 tttttattgc aagtagctca ccgatacgca tgccattagc aatttggaac tgcactatag
    14941 attttacctt tttataatta cgttttctcg ttggatattt ttatgctgaa ttagatagtc
    15001 gaaacaatcc ggtatttctt tcactacatt atcttttaaa ttgttattac atatagttag
    15061 taacgcatca ctatagggat atctatttta tctattacac atataacttt gaattgctta
    15121 ctattttaaa ttaacaatat atattctcat agaatttgtc caattaagtg tagacgattc
    15181 aacatttaga aggaacatac agagttgctg atagagtgta tacacctaag aggaacatta
    15241 ctcttaataa agaagttgtc actttaaaag aattggatca tatcataaga tttgctcata
    15301 tttcttatgg cttatatatg ggagaacatt tgcctaaagg taacatcgtc ataaatacaa
    15361 agaatggtgg taaatataca ttagagtcgc ataaagagct acaaaaagat agggaaaatg
    15421 taaaaattaa tacagccgat ataaaaaatg taactttcaa acttgtgaaa agtgttaatg
    15481 acattgaaca agtttgaaat taagttaaat tagtatatat agtgttttat cgctaatact
    15541 ttgaaagtta ggtatccgaa ggtgcctaac tttctttgtt atgaccatca tcatatagaa
    15601 aattctttaa tgatgttaat gccgaaattt gcaaaaataa tttctctttt atggttaatc
    15661 aatatgtttt tacattcgac aaccttaaaa ttaacttctt tcaattccat aataaagtct
    15721 ctataaaata acttagttta aaaacgattc gtatctttca gattcaaata ccatcatttt
    15781 ctctttagac ttacatttta attacaatta tgtaagttgt tttcagagac atcctatttt
    15841 atattttgtc agaggtgatt ttttgaaaac gattttaaaa acaataacat atcttgcact
    15901 tactatcatt ggcgcttatg ctgctttatt cattttaaaa acaatagact ctcatggtat
    15961 aacagatcaa tttaacccat tagtaaagga agatgattct tatgttaaaa cgacagaggt
    16021 gtctactaga atggatgatc aactccgaag ttatagtcaa agtgctttta ataaagaagg
    16081 gaaagagacg caattaatat atactgctac atttgatgtt aaaccgcata gatacttaaa
    16141 aattacacat aagggtcatc atgtagaaac ttttgaagaa gttgaaaagg aagaagtacc
    16201 aaagaaagca ttagacaaac tgagtcgata atagcatcct tatattacat ggttcatttt
    16261 aaagatgagt acgaaatgaa gtaacgcatg acgattaatt taaaaatatt tgtaataatt
    16321 atgaatagaa ttaaaaacaa ggggtaatac aatctatata gcatataagc ttttgttatg
    16381 agtttcaaaa aaggaagaga gagtgatatt atgaaattaa aatcattagc agtgttatca
    16441 atgtcagcgg tggtgcttac tgcatgtggc aatgatactc caaaagatga aacaaaatca
    16501 acagagtcaa atactaatca agacgctaat acaacaaaag atgttattgc attaaaagat
    16561 gttaaaacaa gcccagaaga tgctgtgaaa aaagctgaag aaacttacaa aggccaaaag
    16621 ttgaaaggaa tttcatttga aaattctaat ggtgaatggg cttataaagt gacgcaacaa
    16681 aaatctggtg aagagtcaga agtacttgtt gctgataaaa ataaaaaagt gattaacaaa
    16741 aagactgaaa aagaagatac agtgaatgaa aatgataact ttaaatatag cgatgctata
    16801 gattacaaaa aagccattaa agaaggacaa aaagaatttg atggtgatat taaagaatgg
    16861 tcacttgaaa aagatgatgg caaacttgtt tacaatatcg atttgaaaaa aggtaataaa
    16921 aaacaagaag ttactgttga tgctaaaaac ggtaaagtat taaagagtga gcaagatcag
    16981 taaaatatgc cgtaacacta atgataataa catgtcttaa ttcata
//
LOCUS       DAPIDM010000046        16412 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000046 DAPIDM010000000
VERSION     DAPIDM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.046"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            180..1136
                     /locus_tag="RJE42_001318"
     CDS             180..1136
                     /locus_tag="RJE42_001318"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV5876746.1"
                     /translation="MLNSFDAAYHSLCEEVLELGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1336..1815
                     /locus_tag="RJE42_001319"
     CDS             1336..1815
                     /locus_tag="RJE42_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDV5876747.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEDVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1830..2669
                     /gene="fakB2"
                     /locus_tag="RJE42_001320"
     CDS             1830..2669
                     /gene="fakB2"
                     /locus_tag="RJE42_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDV5876748.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2755..3288
                     /gene="msrA"
                     /locus_tag="RJE42_001321"
     CDS             2755..3288
                     /gene="msrA"
                     /locus_tag="RJE42_001321"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV5876749.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3281..3709
                     /gene="msrB"
                     /locus_tag="RJE42_001322"
     CDS             3281..3709
                     /gene="msrB"
                     /locus_tag="RJE42_001322"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV5876750.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3721..4221
                     /locus_tag="RJE42_001323"
     CDS             3721..4221
                     /locus_tag="RJE42_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV5876751.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQATSINIYDENAVIKGETKV
                     IDVTMN"
     gene            4221..4442
                     /locus_tag="RJE42_001324"
     CDS             4221..4442
                     /locus_tag="RJE42_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDV5876752.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4627..6117
                     /locus_tag="RJE42_001325"
     CDS             4627..6117
                     /locus_tag="RJE42_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDV5876753.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQQTNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQNSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6518..7027
                     /locus_tag="RJE42_001326"
     CDS             6518..7027
                     /locus_tag="RJE42_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876754.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENNKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGTEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            7039..8109
                     /locus_tag="RJE42_001327"
     CDS             7039..8109
                     /locus_tag="RJE42_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV5876755.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKDDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNKMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            8126..8740
                     /locus_tag="RJE42_001328"
     CDS             8126..8740
                     /locus_tag="RJE42_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDV5876756.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
     gene            8857..9057
                     /locus_tag="RJE42_001329"
     CDS             8857..9057
                     /locus_tag="RJE42_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876757.1"
                     /translation="MTNPTRTKRCGGDVLCGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKNCISQ"
     gene            9134..9266
                     /locus_tag="RJE42_001330"
                     /pseudo
     CDS             9134..9266
                     /locus_tag="RJE42_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9528..10187
                     /gene="arlR"
                     /locus_tag="RJE42_001331"
     CDS             9528..10187
                     /gene="arlR"
                     /locus_tag="RJE42_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDV5876758.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            10184..11539
                     /gene="arlS"
                     /locus_tag="RJE42_001332"
     CDS             10184..11539
                     /gene="arlS"
                     /locus_tag="RJE42_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDV5876759.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIDYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYHFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            11828..14626
                     /locus_tag="RJE42_001333"
     CDS             11828..14626
                     /locus_tag="RJE42_001333"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV5876760.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            14640..15911
                     /gene="sucB"
                     /locus_tag="RJE42_001334"
     CDS             14640..15911
                     /gene="sucB"
                     /locus_tag="RJE42_001334"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV5876761.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNDETTNNK
                     KEETTNKSADKAEVNQTNDDNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDI
                     DKKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAML
                     TTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGD
                     DMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMV
                     NGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSY
                     DHRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         6160 a         2294 c         2925 g         5033 t
ORIGIN      
        1 aacatagatc acgttatgat gtggcaggta cttaatcatc attttataaa taaagtaacg
       61 gaattcatga tattaatatc atattcctaa aatgagtgat aacaaaatgc tacataaagt
      121 taaattatat agaactaaat atacatacta taaataataa aaatgaggtg ttatcgcata
      181 tgttgaattc atttgatgca gcatatcaca gtctttgtga agaagtttta gaactaggga
      241 atacacgaaa tgatcgcaca aatacaggta cgatttcgaa atttggtcat caacttcgct
      301 ttgacttatc taaaggattt ccactattaa cgacaaagaa agtttctttt aaattagtag
      361 caaccgaatt attatggttc attaaaggag atacaaatat ccaatactta ttaaagtata
      421 ataataatat ttggaacgaa tgggcttttg aaaattatat caaatcagac gagtataaag
      481 gtccagatat gacagatttc gggcatcgtg cattgagtga tcctgaattt aacgaacaat
      541 ataaagaaca aatgaaacaa tttaagcaac gtattcttga agatgataca tttgcgaagc
      601 aattcggtga tttaggaaat gtttatggta aacagtggcg agattgggtt gataaagatg
      661 gtaatcattt tgatcaactt aaaacagtaa ttgagcaaat taagcataat ccagattcaa
      721 gacgacacat cgtatctgca tggaatccaa cagaaattga cacaatggct cttccgcctt
      781 gtcataccat gttccagttt tatgtccaag atggtaagtt aagttgccag ttataccaac
      841 gtagcgcaga tatcttttta ggtgtgccat ttaatatcgc aagctacgct ttattgacac
      901 accttattgc caaagaatgt ggccttgaag taggagaatt tgtgcatact tttggagatg
      961 cacatattta ttcaaatcat attgatgcga ttcaaacaca attagcacgt gaaagcttca
     1021 atcctccaac attaaaaatt aacagtgata agtctatttt cgacataaat tatgaagatt
     1081 tggaaattgt tgactatgaa tcacatccag caataaaagc tccaatagca gtgtagtcgt
     1141 tgcatagtta gctaaccata tagacatcaa aatgacatca aagtattttc aagtgcaaaa
     1201 aagtactttt ttgtgttaaa cgttttcata aattatgcaa aatcattatt tctatcacac
     1261 tttatgataa aaattgtgtt aaattaaaga taacttagta ataaaaaatg aaatgataga
     1321 agaaggagga taattatgac tttatccatt ctagttgcac atgacttgca acgagtaatt
     1381 ggttttgaaa atcaattacc ttggcatcta ccaaatgatt tgaagcatgt taaaaaatta
     1441 tcaactggtc atactttagt aatgggtcgt aagacatttg aatcgattgg taaaccacta
     1501 ccgaatcgtc gaaatgttgt acttacttca gatacaagtt tcaacgtaga ggacgttgat
     1561 gtaattcatt ctattgaaga tatttatcaa ctaccgggcc atgtttttat atttggaggg
     1621 caaacattat ttgaagaaat gattgataaa gtggacgaca tgtatattac tgttattgaa
     1681 ggtaaatttc gtggtgatac gttctttcca ccttatacat ttgaagactg ggaagttgcc
     1741 tcttcagttg aaggtaaact agatgagaaa aatacaattc cacatacctt tctacattta
     1801 attcgtaaaa aataaggggg aaaacgacca tgacaaaaca gattatagta acagactcaa
     1861 catccgattt atctaaagaa tatttagaag caaacaacat acatgtaatt cctttaagtt
     1921 taactattga aggagcttca tacgttgacc aagtagatat tacatcagaa gaatttatta
     1981 atcatattga aaatgatgaa gatgtaaaga caagtcagcc agccataggt gaatttatat
     2041 ctgcttatga agaactagga aaagatggct ctgaaatcat aagtattcat ctttcttcag
     2101 gactaagtgg tacatttaac actgcttacc aagcaagtca aatggtagat gctaatgtaa
     2161 ctgttattga ttcaaaatct atttcttttg gtttagggta tcaaatacaa cacctagtag
     2221 agcttgtaaa agaaggtgtc tcaacttctg aaatagttaa aaagttaaat catttaagag
     2281 aaaacattaa attatttgta gttatagggc aattgaatca attaattaaa ggtggcagaa
     2341 ttagtaaaac aaaaggtttg attggtaatc ttatgaaaat taaaccaatt ggtacactag
     2401 atgatggtcg cttagagctt gtgcacaatg cgagaactca aaattctagt atccaatact
     2461 tgaaaaagga aattgctgaa tttataggag atcatgaaat caaatccgtt ggtgtcgcac
     2521 atgctaacgt cattgaatat gttgataaat tgaagaaagt ttttaatgaa gcttttcatg
     2581 tgaataatta cgatataaat gtaactacac cagttatttc tgcacatact ggtcaaggtg
     2641 cgattggcct cgtagtcctt aagaagtaaa tttaatcttt tcagtgttaa ttacttccat
     2701 ttcaatcctt tatagactaa atttataatt agatagatag aggaggtaat tcatatgaca
     2761 aaagaatatg caacattagc aggaggatgt ttctggtgca tggttaaacc atttacatca
     2821 tatccaggca tcaagtcagt cgtatctggt tatagtggcg gtcatgttga taacccaact
     2881 tatgaacagg tatgtacgaa tcaaaccggc catgtcgaag cagtacaaat tacgtttgat
     2941 ccagaggtta cttcctttga aaatatatta gacatatatt tcaaaacatt tgacccaacc
     3001 gatgatcaag ggcaattttt cgatagaggc gaaagctatc aaccagtcat tttctatcat
     3061 gatgagcatc agaaaaaggc tgctgagttt aaaaagcaac aattaaatga acaaggtatt
     3121 ttcaagaaac cagtgattac acctattaaa ccatataaaa atttctatcc agctgaagac
     3181 taccatcaag attattataa aaagaacccg gtacattatt accaatatca acgtggttca
     3241 ggtagaaaag cgtttataga atcacattgg gggaatcaaa atgcttaaaa aagataaaag
     3301 tgaactaaca gatatagaat atattgttac acaagaaaat ggcactgaac caccatttat
     3361 gaatgaatat tggaatcatt ttgctaaagg gatttatgta gataagattt ctggtaaacc
     3421 tttatttaca tctgaagaaa agtttcattc tgagtgcgga tggcctagct tttccaaagc
     3481 gcttgatgac gatgaaatta tagaattagt cgacaaatca tttggtatgg taagaactga
     3541 agtgcgttca gaagaatcaa atagtcattt aggacacgtc tttaatgatg gacctaaaga
     3601 aagtggcggc ttaagatact gtatcaattc cgctgcaatt caatttattc catatgaaaa
     3661 attagaagaa ttgggttatg gcgatttaat atcacatttt gataagtagg agtggaaagt
     3721 atgtttaaaa aattattcgg aaaaggcaaa gaagttcaga aagatatcgc aatttatgca
     3781 ccattaactg gagaatacgt gaaaattgaa gatattccag atcctgtatt cgcacaaaaa
     3841 atgatgggcg aaggttttgg tattaatcca actgaaggag aagttgtgtc tccaattgca
     3901 ggacgtgttg acaatgtctt tccaactaag catgcgattg gtttaaaagc agataacgga
     3961 ttagaattat tagttcatat cggtttagac acagttcaat tagacggtga aggatttgaa
     4021 gtgttagtgt ctagtggtga tgaagttaac gtcggagatc cattagtaag attcaacctt
     4081 gaatatatta ataataatgc taaatctgtt atttcaccaa ttataattac taacactgat
     4141 caagcaactt caattaatat ttatgatgaa aatgctgtga ttaaaggtga aactaaagtg
     4201 attgatgtga caatgaacta atgaaaaact attcgtttta tcaatttgtc atgacagttc
     4261 gtggtcgaca cgacgataaa ggtcgtctag cagaagagat atttgacgat cttgctttcc
     4321 caaaacacga tgatgatttt aacatactgt ctgattatat tgagacacat ggtgatttca
     4381 cattgccaat gtctgtattt gatgatctat atgaagaata tacagaatgg ctaaaatttt
     4441 aatataattt ttaatactaa tagttagaac cagggtgatg caagttgtta tcctggtttt
     4501 aatttaaaat aaactaagtt gtgactaaaa attaatcaat tatagtgaaa tatggtgcgc
     4561 tatcttgcat aaattgatat gattaactac acagaattta aaagtacata ataaggaagt
     4621 gatacaatgg atgataagca acacacatct tcatccgatg atgaacgcgc tgaaattgca
     4681 acaagcaatc aagaccaaca aactaattca tcgaaacgcg ttcacttaaa acgttggcaa
     4741 ttcatatcaa ttttaattgg tacaatccta ataacagctg tcatcacagt tgttgcatat
     4801 atttttataa atcaaaaaat aagtggttta aacaaaactg atcaagcaaa cttaaataaa
     4861 attgaaaatg tgtataaaat cttaaatagt gattattata aaaaacagaa ctctgacaag
     4921 ttgagtaaag ctgcaattga tggcatggtc aaagaattaa aagatcctta ttctgaatat
     4981 ttaacaaaag aacaaactaa atcctttaat gaaggtgttt caggtgattt tgtaggtatt
     5041 ggtgcagaaa tgcaaaagaa aaatgatcaa attatggtta ctagccctat gaagggttct
     5101 ccagcagaac gtgctggcat tcgtcctaaa gatgtcatta ctaaagtaaa tggaaaatca
     5161 attaaaggaa aagcattaga cgaagttgtc aaagacgttc gcggtaaaga aaacactgaa
     5221 gtaactttaa ctgttcaaag aggcagtgaa gaaaaagacg ttaagattaa acgtgaaaaa
     5281 attcatgtta aaagcgttga gtataagaaa aaaggtaagg ttggagttat tactattaat
     5341 aaattccaga atgatacatc aggtgaattg aaagatgcag ttcttaaagc tcacaaagat
     5401 ggtttgaaaa agattgtttt agatttaaga aataatccag gtggactact agatgaagct
     5461 gttaaaatgg caaatatttt tatcgataaa ggaaaaactg ttgttaaact agaaaaaggt
     5521 aaagatactg aagcaattca aacttctaat gatgcgttaa aagaagcgaa agacatggat
     5581 atatccatct tagtgaatga aggttcagct agcgcttctg aagtgtttac tggtgcgcta
     5641 aaggactata ataaagctaa agtttatggg tcaaaaacat tcggcaaagg tgtcgtacaa
     5701 actacaagag agtttaagga tggttcgttg ttaaaatata ctgaaatgaa atggttaacg
     5761 cctgatggtc attatattca cggtaaaggt attaaaccag acgttactat tgacacacct
     5821 aaatatcaat ctttaaatgt cattcctaat acgaaaacat ttaaagttgg agacgatgat
     5881 aaaaatatta aaactattaa aattggttta tcagctttag gttataaagt tgataatgaa
     5941 tcaacacaat ttgatcaagc tttagaaaat caagttaaag ctttccaaca agcgaataaa
     6001 ctcgaggtaa ctggtgaatt taataaagaa acgaataata aatttactga gttattagtt
     6061 gaaaaagcta ataaacatga tgatgttctc gataaattga ttaatatttt aaaataaagc
     6121 gatacacact actaaaattt tattattatt atgttaatga cacgcttcct aaatttgcaa
     6181 agatagcaat ttaggaggcg tatttatttt tattgacgtc taactctaaa agatataaat
     6241 tagacattta caaatgatgt aaataacaca atttctatca tcgctgttaa caattcatgc
     6301 tttaatatac tatgagcata tagtttttaa atagtattat tcactagttt taacaatcaa
     6361 ttaattggta tatgatactt ttattgatta tttttatccc atagtgtgat aattactatt
     6421 tttcattcat aataaaggtt taaagcatgc taatagtgtg taagattaac atgtactgaa
     6481 aaacatgttt aatataacga tataaggagt gacgtacatg atccgtctag gtaaaatgtc
     6541 agatttagat caaatcttaa atctagtaga agaagcaaaa gaattaatga aagaacacga
     6601 caacgagcaa tgggacgatc agtaccctct tttagaacat tttgaagaag atattgctaa
     6661 agattatttg tacgtattag aggaaaataa taaaatttat ggctttattg ttgtcgacca
     6721 agaccaagca gaatggtatg atgacattga ctggccagta aatagagaag gtgcctttgt
     6781 tattcatcga ttaactggtt cgaaagaata taaaggagct gctacagaat tattcaatta
     6841 tgttattgat gtagttaaag cacgtggtac agaagttatt ttaacggaca cctttgcgtt
     6901 aaacaaacct gcacaaggtt tatttgccaa atttggattt cataaggtcg gtgaacaatt
     6961 aatggaatat ccgccatatg ataaaggtga accattttat gcatattata aaaatttaaa
     7021 agaatagagg taatattaat gacgaaaatc gcatttaccg gagggggaac agttggacac
     7081 gtatcagtaa atttaagttt aattccaact gcattatcac aaggttatga agcgctttat
     7141 attggttcta aaaatggtat tgaaagagaa atgattgaat cacaactacc agaaattaag
     7201 tattatccta tttcgagtgg taaattaaga agatatattt ctttagaaaa tgccaaagat
     7261 gtatttaaag tattaaaagg tattcttgat gctcgcaaag ttttgaaaaa agaaaaacct
     7321 gatctattat tttcaaaagg tggatttgta tctgtgcctg ttgttattgc agccaaatca
     7381 ttaaatatac caactattat tcatgaatct gacttaacac caggattagc gaataagata
     7441 gcacttaaat ttgccaagaa aatatataca acatttgaag aaacgctaaa ctacctacct
     7501 aaagagaaag ctgattttat tggagcaaca attcgagaag atttaaaaaa tggtaatgca
     7561 cataatggtt atcaattaac aggctttaat gaaaataaaa aagtgttact cgttatgggt
     7621 ggaagcttag gaagtaaaaa attaaatagc attattcgcg aaaacttaga tgcattatta
     7681 caacaatatc aagtgataca tttaactggt aaaggattaa aagatgctca agttaaaaaa
     7741 tcaggatata tacaatatga atttgttaaa gatgatttaa cagatttatt agcaattacg
     7801 gacacagtaa taagtagagc agggtcaaat gcgatttatg agttcttaac attacgtata
     7861 ccaatgttat tagtaccatt aggtttagat caatcccgag gcgaccaaat tgacaatgca
     7921 aatcattttg ctgataaagg ttatgctaaa acgattgatg aagaacaatt aacagcacaa
     7981 attttattac aagagctaaa taaaatggaa caggaaagaa ctcgaattat caataatatg
     8041 aaatcgtatg aacaaagtta tacgaaagaa gctttatttg ataagatgat taaagacgca
     8101 ttgaattaat ggggggtaat gctttatgag tcaatggaaa cgtatctctt tgctcatcgt
     8161 ttttacattg gtttttggaa ttatcgcgtt tttccacgaa tcaagactag ggaaatggat
     8221 tgataatgaa gtttatgaat ttatatattc atcagagagc tttattacga catctatcat
     8281 gcttggggct actaaagtag gtgaagtctg ggcaatgtta tgtatttcat tacttcttgt
     8341 ggcatatctc atgttaaagc gccacaaaat tgaagcatta ttttttgcat taacaatggc
     8401 attatctgga attttgaatc cagcattaaa aaatatattc gatagagaaa gacctacatt
     8461 gctgcgttta attgatataa caggatttag ttttcctagc ggtcatgcta tgggatcaac
     8521 tgcatatttt ggaagtggta tctatttatt aaatcgatta aatcaaggta attcaaaagg
     8581 tattcttata gggttatgtg cagctatgat tttattgatt tccatatcac gtgtatatct
     8641 aggtgtacat tatccaacag atattattgc cggcattatt ggtggcgtat tttgcattat
     8701 tttatcaacg ttattactta gaaataaatt aatcaattaa atagtaaaaa aacaaaagca
     8761 gtaaacctaa agtgtcgtaa gggtttactg cttttataaa acgttgttat aacgtatatt
     8821 gtcttttacg ggcatataaa aggggaatat ttgaaaatga ccaatccaac aagaacgaaa
     8881 cgttgtgggg gggatgttct atgtggtatt gataatcatt ttcaactact attatacatt
     8941 agtgagaatc attgtcaatt agaaactaaa actttttttg aatatttttt aagaatagta
     9001 aataaaacgc atgattacgc tattttagaa aataaaaaaa attgtatttc tcagtagaat
     9061 tagaatattt aaaagtgatg aggcttaaaa attatattgt ttacatactt cttttgaatt
     9121 catacattaa gaaatgttac ttgtaagttc aaaatcgcac attgaaatga tgtgtgacaa
     9181 tgtttaaact acggtcattt tgtgaaaata aagataataa ctattcattt tacaatagtg
     9241 aaaagtcagt atatgacaac aattaatatt gcggtaaggc cttgtgttac agtattctat
     9301 atttaagtac tgcaatcaga attaacagaa tgccattaac tgattattaa atatttgagt
     9361 taataaataa ttaatgattg tagcttgaaa aatttaaaac atggttattg atttgtgata
     9421 aaatttaaac gtaaacaaac taatttaaaa agcaactatt gtatggaaaa atacaaaatt
     9481 taaaatatat taccttatta gaaaaagtcg taatatgagg tgtacaaatg acgcaaattt
     9541 taatagtaga agatgaacaa aacttagcaa gatttcttga attggaactc acacatgaaa
     9601 attacaatgt ggacacagag tatgatggac aagacggttt agataaagcg cttagccatt
     9661 actatgattt aatcatatta gatttaatgt tgccgtcaat taatggctta gaaatttgtc
     9721 gcaaaattag acaacaacaa tctacaccta tcattataat tacagcgaaa agtgatacgt
     9781 atgacaaagt tgctgggctt gattacggtg cagacgatta tatagttaag ccatttgata
     9841 ttgaagaact tttagcaaga atacgtgcga ttttacgtcg tcagccacaa aaagatatta
     9901 tcgatgtcaa cggtattaca attgataaga atgcttttaa agtgacggta aatggcgcag
     9961 aaattgaatt aacaaaaacg gagtatgatt tactatatct tctagctgaa aataaaaacc
    10021 atgttatgca acgagaacaa attttaaatc atgtatgggg ttataatagt gaagtagaaa
    10081 caaatgtcgt agatgtttat ataagatatt tacgaaacaa gttaaaacca tacgatcgtg
    10141 acaaaatgat tgaaacagtt cgtggcgttg ggtatgtgat acgatgacaa aacgtaaatt
    10201 gcgcaataac tggattattg ttaccacgat gattacgttt gtcacgatat ttttgttttg
    10261 tttaattatt atctttttct tgaaagatac actgcataat agtgagcttg atgatgcaga
    10321 aagaagctca agcgatatta ataatttatt tcattctaag cctgttaaag atatatctgc
    10381 attagacttg aatgcatctt taggtaattt tcaagagatt attatttatg atgagcataa
    10441 taataaatta tttgagacat cgaatgataa cacagtgaga gttgaacctg gttatgaaca
    10501 ccggtatttt gaccgcgtaa taaaaaaacg ttataaaggc attgattatt taattattaa
    10561 agaaccaatc acaacgcaag atttcaaagg gtatagtttg ttaattcatt cactagaaaa
    10621 ttatgataac atcgtaaaat cattgtatat tattgcgctc gcatttggag tgattgcaac
    10681 aattataact gccacaatta gttatgtatt ttcaactcaa attactaaac cgcttgtcag
    10741 tttatcaaat aaaatgattg agattcgacg agatggtttt caaaataaat tgcaattaaa
    10801 tacaaattat gaagaaatag ataatttagc aaatacgttt aatgagatga tgagccaaat
    10861 tgaagaatca tttaatcaac aaagacaatt tgttgaagat gcgtcacatg aattacgaac
    10921 accattacaa attattcaag gtcatttaaa tttgattcag cgatggggaa aaaaagaccc
    10981 agcagtatta gaagaatcgt taaatatttc tattgaagaa atgaatcgta tcataaaatt
    11041 agtcgaagaa ttacttgaat tgactaaagg agatgtaaat gacatttctt ctgaagcgca
    11101 gaccgtgcat attaatgatg aaattcgctc gcgaatacac tcattaaaac aattgcatcc
    11161 tgattatcat tttgatacgg atttgacatc taaaaatcta gaaattaaaa tgaaacctca
    11221 tcaattcgaa caattatttt taatctttat tgataatgca atcaaatatg atgtgaagaa
    11281 taagaaaatt aaagttaaga caaggttaaa aaataagcaa aaaataattg aaattacaga
    11341 tcatggaatt ggtattccag aggaagatca agatttcatt tttgatcgct tttatcgagt
    11401 ggataaatct cgttcaagaa gtcaaggcgg taatggactc ggattatcta ttgcgcaaaa
    11461 aatcattcaa ttaaacggtg gatcgattaa aattaaaagt gaaattaaca aaggaacaac
    11521 gtttaaaatc atattttaat catgtctaag acgtcaatca aagtcatagg atcaattttt
    11581 taagtacaca ttagctgtga ctaatgtata agaacaacta taaaacaaat aaacagtggt
    11641 tctttatcat ttctgtggaa ttcacctccc aaaatttaca ataaaatata tctataaacc
    11701 tagaagaatc aacgcttttg ttgattcttc tttttagtag ataaataggt aaatctactt
    11761 taacaaataa ctaaatagtg atattattac attgtaagcg tttcaacatt tttgtggagg
    11821 gtgtaaaatg actaacgaaa gaaaagaagt ttcagaggct cctgtaaact tcggtgcgaa
    11881 tttaggtcta atgttagatc tatatgatga ctttttacaa gatccatcat ctgtaccaga
    11941 agatttacaa gtcttattca gcacaattaa gaatgatgac tcaattgtac cagctttaaa
    12001 aagtacaagt agtcaaaata gcgacggcac aattaagcgt gtcatgcgtt tgattgataa
    12061 tattcgccaa tacgggcatc ttaaagccga tatttatcct gtaaatcctc cgaaaaggaa
    12121 acatgtacct aaattagaga ttgaagactt tgatttagat caacagactt tggaaggtat
    12181 atcagcagga attgtttcag atcactttgc cgacatttat gataatgctt atgaagcaat
    12241 tttaagaatg gaaaaacgtt acaaaggacc aattgcattt gagtatacac atattaataa
    12301 caataccgaa cgtggttggt taaaaagaag aattgaaacg ccatataaag taacgttaaa
    12361 taataacgaa aaaagggcac tatttaaaca attagcgtat gttgaagggt ttgaaaaata
    12421 tcttcataaa aacttcgttg gtgcaaagcg tttttcaatt gaaggggtag acgcacttgt
    12481 accgatgttg caacgtacta ttacgattgc tgctaaagaa ggtattaaaa atatacaaat
    12541 aggcatggct caccgtggac gtttaaacgt tttaacgcat gtcttagaaa aaccgtacga
    12601 aatgatgatt tcagaattta tgcatacaga tccaatgaaa tttttacctg aagatggtag
    12661 cttgcagtta actgctggat ggactggtga tgtgaaatat caccttggtg gtattaaaac
    12721 tactgattca tacggtacaa tgcagcgtat tgcactggct aacaatccaa gtcacttgga
    12781 aattgttgca cctgttgttg aagggcgtac aagagcagca caagatgata cacagcgtgc
    12841 tggggctccg acgactgatc atcataaagc aatgccaatt attatacacg gcgatgctgc
    12901 ttatcctggt caaggaatta acttcgaaac aatgaactta ggaaacttga aaggctattc
    12961 tacggggggt tcattgcata ttattactaa caatagaatt ggatttacta cagaaccaat
    13021 tgatgcacgt tcaacaactt attctacaga tgtggccaaa ggttatgatg tgccaatatt
    13081 ccatgtcaat gcagatgacg ttgaagctac tattgaagca attgatattg caatggaatt
    13141 tagaaaagag tttcataaag acgtcgttat tgatttagta ggttatcgtc gtttcggaca
    13201 taacgaaatg gatgaaccat caattactaa tccagttcct tatcagaata ttcgcaaaca
    13261 tgactctgtt gaatatgtgt ttggtaaaaa gcttgttaat gaaggtgtca tttcagaaga
    13321 tgaaatgcat tcatttatag aacaagtcca aaaggaacta agacaagctc atgataaaat
    13381 taataaagct gataagatgg ataatccaga tatggaaaag cctgcagatc ttgcattacc
    13441 gttacaagca gacgaacaat catttacgtt tgatcacttg aaagaaataa atgatgcatt
    13501 gttaacatat ccggatggct ttaacatttt gaaaaagtta aacaaagttc ttgagaagcg
    13561 tcatgagcca tttaataaag aagatggttt agttgattgg gcacaagcag aacaacttgc
    13621 atttgcgaca attttacaag atggtacacc gattcgctta actggtcaag atagtgaacg
    13681 tggtacattc agtcataggc atgccgtgtt acatgatgag caaacaggtg aaacatatac
    13741 acctttacat catgttcctg atcaaaaagc gacatttgat atacacaatt ctccgctttc
    13801 agaagcagca gtagttggtt ttgaatacgg ctataatgtg gaaaacaaaa aaagcttcaa
    13861 tatttgggaa gcacaatatg gtgattttgc aaatatgtca caaatgattt ttgacaactt
    13921 cttattcagt tctcgctcaa aatggggaga acgttcagga ttaacattat tcttacctca
    13981 tgcatatgag ggtcaagggc ctgaacattc atcagcaaga ttagagagat ttttacaatt
    14041 agctgctgaa aataattgca cagttgtcaa cttatctagt tcaagtaatt atttccactt
    14101 attgcgtgca caagcggcta gtttagattc tgaacaaatg cgaccattgg ttgttatgtc
    14161 accaaaaagc ttactgagaa ataaaacagt tgcaaaacca attgatgaat ttacttctgg
    14221 tggatttgag ccaattttga cagaatcata tcaagcggat aaggttacaa aagttatttt
    14281 ggcaactggt aaaatgttca ttgatttaaa agaagcatta gctaaaaatc cagacgaatc
    14341 agtattactc gttgcgattg aaagattgta tccattccca gaggaagaga ttgaagcatt
    14401 actagcacaa ttgccaaacc ttgaagaagt gtcatgggta caagaagaac ctaaaaatca
    14461 aggtgcctgg ttatatgttt atccatatgt taaagtgctt gttgcagaca aatatgattt
    14521 aagttatcat ggcagaattc aaagggctgc tccagctgaa ggcgatggag aaattcataa
    14581 acttgttcaa aataaaatta tagaaaatgc attaaaaaat aactaggggg aaataagtca
    14641 tgccagaggt taaagttcca gaattagcag aatctattac agaaggtacc attgcagaat
    14701 ggttgaaaaa cgtaggggat agcgtagaaa aaggtgaagc tattcttgaa ttagaaactg
    14761 ataaagttaa tgtcgaagtt gtatctgaag aagcaggtgt attatctgaa caacttgcaa
    14821 gtgaaggcga cactgtagaa gttggacaag caattgctgt catcggcgaa ggtagtggca
    14881 atgcttctaa agaaaatagt aacgacaata ctccacaaca aaatgatgaa acaacaaata
    14941 ataaaaaaga agaaacaaca aataaatcgg cagataaagc tgaagtcaat caaacaaatg
    15001 atgataatca gcagcgtgtt aatgctacgc cttctgcgcg tcgatatgct cgtgaaaacg
    15061 gtgtgaatct tgctgaagta agtccgaaaa caaatgatgt tgttcgtaaa gaagatattg
    15121 ataaaaaaca acaggcaccg gcatcaacac aaacaacaca acaagcacct gcaaaagaag
    15181 agaaaaaata caatcaatat cctacaaaac cagtgattcg cgaaaaaatg tcacgcagaa
    15241 agaaaacagc tgctaaaaaa ttattagagg tatctaataa tacagctatg ttaacaacat
    15301 ttaacgaagt tgacatgaca aatgttatgg aattgcgtaa acgtaagaaa gaacaattta
    15361 tgaaagatca tgatggtact aaattaggat ttatgtcatt ctttactaaa gcttctgtag
    15421 cagctttgaa aaagtatcca gaagttaatg cagaaatcga cggcgacgac atgattacga
    15481 aacaatatta tgacattggt gtagctgttt ctacagatga tggactatta gtaccattcg
    15541 taagagattg tgataaaaag aattttgcag aaatcgaagc agaaattgct aatttagctg
    15601 ttaaagcacg agagaaaaaa cttggcttag atgatatggt taatggttca tttacgatta
    15661 caaatggcgg tatttttgga tcaatgatga gtacgccaat tatcaatggt aatcaagctg
    15721 caatcttagg catgcattca attattacaa gaccaattgc gattgatcaa gatacaattg
    15781 aaaatcgtcc aatgatgtat attgcattaa gctatgatca tagaattatt gacggtaaag
    15841 aagcagttgg attcttaaaa acaattaaag aattaattga aaacccagaa gacttattat
    15901 tagaatctta atccacaaca caaaatagtt tactattcct aaaaatggga tcaaatcaat
    15961 aacaaacagc agtaagatta ttttctagtc gaaatatctt actgctgtac tttattttta
    16021 caatgcttcg tattaaaatg tgagtttaat tttgactact taaaaaatat atcatttcta
    16081 ttgaaataga ctcacaatag aaatataggt agaatatcgt gtttcaatat gctaatgaat
    16141 tcattttaga taataacaag cgatgaaatt tatgaattct ataagcgcct ttagttaatt
    16201 taaaatcatc tgaacaagat gtaaattcac tttatggaca cgacatatat atgtttttca
    16261 tgtcctacaa aaaacgaaat attcaattgc tatatacaga tattcattaa tgacacactc
    16321 actagcaacc ataacaatat tatgttacct atttaaatga aatagttctt ttaaagaaat
    16381 acatttttca catattaatc tataatcaaa at
//
LOCUS       DAPIDM010000047        16318 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000047 DAPIDM010000000
VERSION     DAPIDM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.047"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(123..629)
                     /locus_tag="RJE42_001335"
     CDS             complement(123..629)
                     /locus_tag="RJE42_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876762.1"
                     /translation="MIIKILTILLLLCILSYLVTNKKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            1303..1707
                     /locus_tag="RJE42_001336"
     CDS             1303..1707
                     /locus_tag="RJE42_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDV5876763.1"
                     /translation="MKKNITKVIIASTVIATGLLTHSNDAKAFFSYEWKGLEIAKKLA
                     DQAKQEEERIDKLMKEADKNQKPYKGETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSIYWDQ"
     gene            1664..1756
                     /locus_tag="RJE42_001337"
     CDS             1664..1756
                     /locus_tag="RJE42_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876764.1"
                     /translation="MLKTVKSTRSIGINKHTELNSCTPNVGKQC"
     gene            complement(2056..2373)
                     /locus_tag="RJE42_001338"
     CDS             complement(2056..2373)
                     /locus_tag="RJE42_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HDV5876765.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKAEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
     gene            complement(2567..2734)
                     /locus_tag="RJE42_001339"
                     /pseudo
     CDS             complement(2567..2734)
                     /locus_tag="RJE42_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2864..3367)
                     /locus_tag="RJE42_001340"
     CDS             complement(2864..3367)
                     /locus_tag="RJE42_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV5876766.1"
                     /translation="MSKWIVVSDNHTESGILYQIYEAHPDADMYLHLGDSEFEYDDTE
                     LSLFRRVKGNCDFYPEFENEAVAKCNDVKAFYTHGHLYQVNRTRHLLAEQARELGCVF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEESYAEVLIDDQTLQGTVNFKNRQHE
                     TISHTTF"
     gene            complement(3360..3947)
                     /locus_tag="RJE42_001341"
     CDS             complement(3360..3947)
                     /locus_tag="RJE42_001341"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV5876767.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DRTMAQLSKEQKGQISHRRNAINLLEAYLAGDQNV"
     gene            complement(3959..4759)
                     /gene="racE"
                     /locus_tag="RJE42_001342"
     CDS             complement(3959..4759)
                     /gene="racE"
                     /locus_tag="RJE42_001342"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV5876768.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYNDPTI
                     TSIVIHQTLKRWRNSKADTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDTTHITNIIKEWLNLSVNVERISVND"
     gene            complement(5000..5815)
                     /gene="sdhB"
                     /locus_tag="RJE42_001343"
     CDS             complement(5000..5815)
                     /gene="sdhB"
                     /locus_tag="RJE42_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV5876769.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(5815..7581)
                     /gene="sdhA"
                     /locus_tag="RJE42_001344"
     CDS             complement(5815..7581)
                     /gene="sdhA"
                     /locus_tag="RJE42_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV5876770.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(7633..8247)
                     /locus_tag="RJE42_001345"
     CDS             complement(7633..8247)
                     /locus_tag="RJE42_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDV5876771.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(8571..10352)
                     /gene="uvrC"
                     /locus_tag="RJE42_001346"
     CDS             complement(8571..10352)
                     /gene="uvrC"
                     /locus_tag="RJE42_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV5876772.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKEMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFIDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            10518..10613
                     /locus_tag="RJE42_001347"
     CDS             10518..10613
                     /locus_tag="RJE42_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876773.1"
                     /translation="MLDYHLTFLDLLIPPFSSRKQYDDLSFNFIY"
     gene            complement(10676..10990)
                     /gene="trxA"
                     /locus_tag="RJE42_001348"
     CDS             complement(10676..10990)
                     /gene="trxA"
                     /locus_tag="RJE42_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDV5876774.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(11163..13511)
                     /locus_tag="RJE42_001349"
     CDS             complement(11163..13511)
                     /locus_tag="RJE42_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDV5876775.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKRADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEDAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRVGMPSEGGFGVTVATLK"
     gene            complement(13521..15233)
                     /gene="polX"
                     /locus_tag="RJE42_001350"
     CDS             complement(13521..15233)
                     /gene="polX"
                     /locus_tag="RJE42_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDV5876776.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTFSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMHYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(15306..15827)
                     /locus_tag="RJE42_001351"
     CDS             complement(15306..15827)
                     /locus_tag="RJE42_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDV5876777.1"
                     /translation="MIIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQHRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(15828..16094)
                     /gene="zapA"
                     /locus_tag="RJE42_001352"
     CDS             complement(15828..16094)
                     /gene="zapA"
                     /locus_tag="RJE42_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV5876778.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         5146 a         3026 c         2220 g         5926 t
ORIGIN      
        1 atcagcactt atatattagt caaaaaatca tacctctact ttttcgaatt ttaaaaataa
       61 aactacacaa caaaaaagac accatttgaa tgttggtgtc ttatagtatc atgtcattaa
      121 atttatttgt aacttctcat tctatagtta aatattgtaa cccagctaat aatcaaagtt
      181 ggtagaatta ctaaattcaa attagacacg tcaaaaaaga ctgattggaa attgtcgata
      241 ttaatatcat taatgtccat tttttcattt actgcttgat agatcgcaaa ataaactaag
      301 aaccattgaa ttaaatgttc taaaacgaca acctctgtta aatcgacatt cttttttcta
      361 aaaagtaatc taactataaa taattggaat atacttgaaa tcaagatcaa tataataccc
      421 attaagaaaa aactaccaaa atcaattgtg ataaattgaa taataccata aataattacg
      481 gcagatattg ccagtgaaat atatcctata aagataaaca ccacacccat aaagagtgtc
      541 tttaagaaca gaaaaggctt ctttttattt gtaaccaaat agctcaatat acaaagtagt
      601 aatagaattg ttaaaatttt aattatcatc gtttgacctc aatatattta tttatatctt
      661 tattattgac cacgaaaaaa taacaatcac ttaagtgatt gccaataata ctcaatgcct
      721 actctatagt aatcttggca cgtgatttta cactatttta tctatttaaa agtgtaagtg
      781 ttgaattatg cttacttcga aaatgcataa tacaactaaa tagtatattt tttaacttat
      841 atattaatca taacatatca accttaatta gaatatatct cttatttctt tataagtaaa
      901 attatacata ttaattactt ctctactatg aatcatttct ttgttttata aacattaatt
      961 atcaaaacag ctacatgtat aacactttgg agtgtttgtc tacccatcat tgcaaataaa
     1021 atagtagaga aatataaata tcgttttaac aataaaaatc attcaatcat agttgagtga
     1081 tgtttactaa tattattata ctatcaataa acccaataat acctcaaata aaaataataa
     1141 tatcaaattc atcaatattc tttttcaatt gcattaaacc ttattaattt gcacttaaat
     1201 ttaattaatg aagtgttaac tggtaaatgc ttgaaaccgt ttgtaaaata gcagtgtact
     1261 cgcattagta agtacaaatc tattttacag ggagaactta ttatgaaaaa aaatattaca
     1321 aaagtaatta ttgcatctac agtcatcgct acaggtcttc taactcattc aaatgatgct
     1381 aaagctttct ttagttatga atggaaaggt ttagaaattg ctaaaaaatt agcagatcaa
     1441 gctaaacagg aagaagaacg tattgataag ttaatgaaag aagctgataa aaatcaaaaa
     1501 ccttacaaag gtgaaactgt taatgatctg taccttattg ttaaaaaatt aagccaaggt
     1561 gatgtgaaga aagcagttgt cagaattaaa gatggtggtc ctagagatta ctatactttt
     1621 gacttaactc gtcctttaga agagaacaga aaaaatatta aagttgttaa aaacggtgaa
     1681 atcgactcga tctattggga tcaataaaca cactgaatta aatagttgca cgccaaacgt
     1741 tggaaaacaa tgctaacgtt ttggcgtgct tttttatttt aagcaactcc caacttattt
     1801 atcatcttta cgactgttta ataaagcacg tatgattaac acaatacctc taaatccgcg
     1861 aaaattctaa tgattgttaa tacatatgct tggcgttatc tcgtcattca aaacacctat
     1921 cacatacatt ttcaaacgat tttctaagat ttaccccaat atactcatca agtaaaatac
     1981 atacaatgtt taaataactt tcatcagatt acacatagta aaaaaagcca cactctatag
     2041 catggcttct atcaattatt ttaaagtatt atattttaaa actagatcga tttgtctttg
     2101 taattttttc ttttcatagc tgtgctggaa atgaattaag ttaacagctc tttgtgcttt
     2161 acggtgtgtt gcaacagttc ttgtacgttt gaacaagttt acagcttttt gtgcatccac
     2221 aactttttta tttacttgtt ttttaaagtt tgtttgatac tcgttatatt tttcagcctt
     2281 agtttgtgca tgagattcac cggcaaatgt tgataccgtt aaactaatag cagctatgct
     2341 taaaattgat ttcccaataa aattcttttt cataattaat aacacctatc ctcaaatatt
     2401 tattttgtat acatttataa taatagacag atgcaaaaaa ctacattaac tatttattaa
     2461 ctaaacatta atatttgtgt cactacattt ctaaatttca agaattttgc tactccatat
     2521 tttaaccacc aaattattga tataatttat gcctttagaa atccttttaa atatgtatac
     2581 gattcatgat tttaactaaa atttcaaatt tcctgacttc ttggaatttt tattttatac
     2641 tttttaataa tacactttta accaaataat caacatgatt caaccatata ggacaatcaa
     2701 caatcacttt cccaaaaagt acaattcttt tcattacata aatgtgttat tcattccagt
     2761 taccttctaa aaattgtcag caacactttt aaacaaacat tcatgtcacg acctcctagt
     2821 taaatcatgc cgcaaaataa aaagagccat agctactttt aatttaaaaa gtagtatgac
     2881 tgattgtttc atgttgtcga tttttaaaat tcactgtacc ttgtaatgtt tgatcatcaa
     2941 tcaaaacttc cgcatacgat tcttccattg aacttctaga ttgagatata cttccaggat
     3001 taataacatg aacaccatta atatactcat attttgcaac atgtgtatgt ccataaaaag
     3061 caaatacaca acctaattca cgtgcttgct cagctaataa atgtcttgtt cgattgactt
     3121 gatataaatg tccatgagtg taaaatgctt tcacgtcatt acattttgcg accgcttcat
     3181 tttcaaattc tgggtaaaaa tcacaattgc cttttaccct acgaaataag ctaagttctg
     3241 tatcatcata ctcaaattct gaatcgccta aatgcaaata catatcagca tctgggtgtg
     3301 cctcgtaaat ttggtataaa atgccagatt cagtatggtt atcactcaca acaatccatt
     3361 tagacatttt gatcacccgc aagataagct tctaataaat taattgcatt tcgtctatgg
     3421 ctaatttgac ctttttgttc tttagaaagt tgcgccatgg ttctatctaa tttcggtaca
     3481 taaaatatcg gatcatatcc gaaaccattt tcgccatatt tcccatctgc aatttcacct
     3541 gaaacagtac ctttaaatac ttttgtttcc atatcagggc cactcatact tatgacacaa
     3601 acaaattgcg cacgacgatc agttgtatta ccaagcttat ttaataattt ttcaatattt
     3661 gcttcatcgc ttttattttc accagcataa cgtgcagagt atatacctgg ctcaccattt
     3721 aatgcaaaaa cttctagtcc actgtcatca gctatgaccg ttttattcaa tgcttttgca
     3781 gcagcttctg attttaatat agcattttct tcaaatgttg atcctgtttc ttccacatca
     3841 aaatctggta ttagttctga aatacctatt acgtggtaat ctggaaatat cactttaaaa
     3901 tcatttattt ttccttgatt attcgatgca ataacaatct ctttcattaa aaatcctcct
     3961 agtcattcac tgatatacgt tccacattga cagataaatt tagccactct ttgataatgt
     4021 tagtaatatg tgtggtatca cctgttgcaa aaaatcgatg atctggatgt tcagtataac
     4081 ttgcatgttc attactaaat gttagcaatg cactaacttc acgagctgtt tctaatcctg
     4141 acgaaatcac tgtctttttg ccaccaaaat aatcatagat aggtttatag agcaatggat
     4201 agtgtgtaca acctaaaata accgtatctg ctttactatt tctccatcgt ttcaatgttt
     4261 gatgaatgac gatactcgta atcgtcggat cattgtatcg catttgttct actaatggca
     4321 caaaacctgg acaagctacc ccatgtacct ctacatgtgg attgatacgt ttaatatgtg
     4381 tacgatatgc ttcagactta atagtacctt ccgtacctaa tacaagtaca ttttgatttc
     4441 tagttgtcat aatcgcagtc ctagcacctg gctctattac accaataaca ggaatcgata
     4501 atgttttttg taaatattct aaagctacag cagttgcagt attacaagca atcacgagca
     4561 ttttaatatc aaattccatt aatttacgag cgatttcaac tgtatattgt tttacttgtt
     4621 ctcctggtct tggcccatat ggacatcgcc caatatcacc taagtaataa atcgtctcat
     4681 ttggcaactg acgcataatt tctttagcta ctgtcaaacc tccgacacca gagtctatta
     4741 cacctattgg tttattcata ttcggtcatc cttactttta atttaatttt tagtttatca
     4801 taactaagca ttggatttta gtattatgca ctgtgtttac catttttgtc attataatat
     4861 aatacttgtt ttaaatcagc ccactatcat agtgtcatgt aatctgctta ttaaaaaaat
     4921 cccttctaag ttattgtgta tctccattca atttaatttt gaaaggaaca taacatttta
     4981 actcaaaagg gattaatttt tattctactt catggtctga accaaagaat gatttaaaca
     5041 tgtggaatgt tgtttctctg ttcattgctg caatggatgt tgttaatgga atacctttag
     5101 ggcaagcatt aacacagttt tgtgaattac cacactgctg taagccacct gtacccatta
     5161 atgcatttaa acgttcatct ttagtcatag atcctgttgg gtgcaagtta aacaaacgaa
     5221 cttgcgagat tgcttgcgca ccaacgaatt tattattttc agttacatta ggacaaacct
     5281 ctaaacatac accacatgtc atacatttag ataattcata agctgtttga cgttttttct
     5341 ctggcatacg tggtcccgga cctaaatcat atgttccatc aattgggatc catgctttca
     5401 tacgttttaa gttatcgaac attctagaac gatcaacttg taagtcacgg ataactggga
     5461 aagtattcat tggctctaaa cgaataggtt gttctaattg atcaacaatc gcagaacaag
     5521 attgtcttgc acgaccattg ataaccatag aacatgctcc acatacttct tctaagcagt
     5581 tcatatccca gacaacaggt gttgtttttt cacctttaat attaactggg ttacgtctaa
     5641 tttccattaa acatgcaatg acgtttaaat tttcacgata tggaatttca aatgtttctt
     5701 cataaggctt agaatcactt gtatcttgtc gtttaataat taattttact gttttttgtt
     5761 tcggtttaga ttgtgtttca tgttgtggag tgtttttcac tgattgttca gtcattattt
     5821 tttaccccct ttagacttac ttgtgtaatc acgtttacga ggtggtatta aactcacatc
     5881 gacgtcatca taagtaaact gtggtttttc aaatgcgcct tggaatgagg ccattgtcgt
     5941 ttttaaccac tcttcatcat tacgctctgg gaattctggt ttataatggg caccgcgtga
     6001 ttcgttacgg ttatatgcac caatcgtaat aacacgtgca agtactaaca tgttccataa
     6061 ttgacgagta aagaataccg cttggttact ccaagtttga gtatcttcca tatcaatatc
     6121 ttcatagcgt ttcatcaatt caacaatctt tttatctgtt tctaacagtt tttcattttc
     6181 acgaacaaca gttacatttg ctgtcataat ttcaccaagt tcacggtgta atttatatgc
     6241 attttctgta ccgcgcatag ctaataattt atcaaaacgt tcttgctctt cagctttacg
     6301 tttttcaaaa atactttcgt ccatatcagt atatgatcga tcaatatttg aaatataatc
     6361 aatcgcgttt ggacctgcta ctgtaccacc ataaatcgct gataacaatg aattggcacc
     6421 taagcggtta ccaccatgtt gagagaaatc acattctcca gctgcaaata accctttaat
     6481 atttgtcatt tgatcataat ctacatatag accacccatt gaatagtgga cagctgggaa
     6541 aatcttcatt ggtactttgc gtgggtcatc accagtgaat ttttcataaa tttcaatgat
     6601 accacctagt tttacatcta actcatgtgg atctttatgt gacaaatcaa gatataccat
     6661 gttttcgcca ttaataccta atttttggtt aatacataca tcgaaaattt cacgcgttgc
     6721 gatatcacga ggtactaagt taccataatc aggatatttc tcttctaaga agtaccaagg
     6781 cttaccatct ttatatgtcc aaattcgtcc accttcacca cgtgctgatt cactcattag
     6841 tcgtagttta tcatcaccag ggattgcagt aggatgaatt tgaatgaact caccattagc
     6901 ataaatagcg ccttgttggt aaacaatgga agccgctgat cctgtattaa tcattgagtt
     6961 tgttgtttta ccgaagatga taccagggcc acccgttgcc ataataactg catctgaacc
     7021 aaatgtttca atctcagcag ttgtcatatt ttgtgcaacg atacctcttg cactatcatc
     7081 gtcaccttta actatgccaa ggaattccca tccttcatac ttcgtaacta atccatctac
     7141 ctcatatgca cgaacttgtt catccaatgc atataataat tgttgtccag ttgttgcccc
     7201 tgcatatgct gttctgtggt gtaatgtacc accgaaacgt ctaaaatcta gtagaccttc
     7261 atttgttcta ttgaacatta cgcccatacg gtctaataaa tgaataattt taggtgctgc
     7321 ctctgtcatc gctttaacag gtggttggtt tgcaaggaag tcgccaccat acactgtatc
     7381 atcaaagtga atccaaggag aatcgccttc ccctttagta ttgaccgcac cattaatgcc
     7441 accttgggca caaacagagt gcgaacgttt tactggtaca accgagaata aatctacatg
     7501 tgcacctttt tctgccgctt taattgttga cattaagccc gctaggccac ctccgacaac
     7561 aataagatgt ttctctgcca taaaaatgtc actcccctaa attttcaatc tatttttgtt
     7621 aaatgcgatg tattacataa aggcaataat tgctgtaaca ccaatatacg aaataactaa
     7681 gaatacgatt aatgaaaccc atgtaaatac tcgttgtgat tttggagatt gaagtccacc
     7741 ccaagtaact aagaatgacc ataagccatt tgcaaaatgg aacacaacag caataataca
     7801 aataatataa aatattgccc atccaggatg ttgcaatgtt tcgtgcatta aatcgtaatt
     7861 cacttctttg ccgtaaaatg ctttttgtaa acgtgtttgc cataaatgga taccaataaa
     7921 gatgaatgtt aagataccac tcactctttg gaagaagaac atccagtttc taaaaatcga
     7981 gtaatgtcca acattttctt ttgctgtaaa tgcaatgtgt ataccaaaca aaccgtgata
     8041 taacaacgga atgtatataa ataaaaattc tacaataatt agaaatggta atgattccat
     8101 aaagttagat gccttattaa acgcttcagc accttgtgtt gcttggtgat tcactaataa
     8161 atgaactacc aaaaatgcac ctattgggat aatacctaat aacgaatgaa tacgtcttag
     8221 ataaaattca ttttttgatt gagccaaaag gagtcccccc tgtgaacgaa tatttaattt
     8281 attgagctat ttatattaaa cgtacgctta accccctaaa gtgatatcgt ttctagcgtt
     8341 gtcattatca ttaagcgaaa cattttaaag acaaatacac actgcacgat caccaaactg
     8401 catgtcgaac aatgtaacat ttggattcga tatttaagat tgcttgtgat gataaacttt
     8461 ctcatttaga aaacgcttcc acgtacattc aaaaaaataa ctttgttaac catattgtaa
     8521 cattatttca tatattttgg ggcatgagaa tgattctcac gcccagtaat ttatttatgc
     8581 aattgttcat gtaggttctt tgcgacgttt tcaggaatac ctatattttt aaaatcttca
     8641 agtgtagctt ccttcatttt cttgattgaa ccgaatgaac gcaataataa tgttttacgt
     8701 ttgctaccga taccatctat atcatcaagg attgatttca agcctgtctt ttgacgtgtt
     8761 tgtctatgaa atgtgattgc gaatctgtga acctcatctt ggatacggtg caacaaataa
     8821 aatgcttggc tatttttctt cagtggtaca atttctgcac tagcgccata taataattca
     8881 gatgtttggt gtttatcatt tttttgcaaa cctgcaacag ggatatcaag acctaactcg
     8941 ttttgtagca catcaataac cccgttcata tgtcctttac caccatctac tattattaaa
     9001 tcaggtaatg gtaatccttc gtttaaaacg cgagagtatc ggcgtcttac tacttctctc
     9061 attgatttgt aatcatctgg acctttaacc gttttgattt tatactttct ataatttttc
     9121 ttgtctggtt taccgtcgat aaatgtaacc attgctgaca ctggatccac accttgaata
     9181 ttagaattat cgaatgcttc aattctaatt ggtgtttgaa ttcccatttg tgttccaagt
     9241 tcttcaattg ctttaattgt tctggactca tcacgtgaaa ttaattcaaa tttattattt
     9301 aaggatactt tagcgttatg tgcagctagg tcaaccatct cttttttggg acctcgcgcg
     9361 ggttgaacga ttttagtgtc cacaacagat tgaatcattt ctttatccaa attacgtggc
     9421 acatgaactt ccttaggtaa aatatgttgg tttaagctat aaaattgtcc aataaatgta
     9481 taaaattctt cttcttctgt ttgctgtaat ggaatcatcg ttgtatctcg ttttatcata
     9541 ttaccttgtc ttataaagaa aacttggata cacatccatc ctttatcaac actataacca
     9601 aagacatcac gaatcgtttt atctgatgac ataatttttt gtttgttagt tagattttga
     9661 atatgttgaa ttaaatctct atattcttta gcccgttcaa aatcaagtga ttcacttgca
     9721 gttaacattc gctcttctaa actttttaaa attgttttgt cttccccatt cagaaaatca
     9781 gtaatttcct tcgtcatttg tgcgtattta ctcaaatcaa cgtcatatac acatggtcct
     9841 aaacattgtc caatatggta ataaagacat aatttatctg gcatcttatc acatttgcga
     9901 tatggatata ttctgtctaa taacttttta gtttcttgag cagaatatgc atttggatac
     9961 ggtccgaaat atttgccagt accttgtttt acagttctcg tcactagtag tctaggatat
    10021 ttttcctttg ttattttaat aaatggataa cttttatcat cttttaataa aatattatat
    10081 cttggttgat attgtttaat cagattcaat tccagtaaaa gtgactctgt ttcacttgac
    10141 gtgacaataa actcaaagcg acgtatttca ccaaccagtc tcgttgtttt agcatcatga
    10201 gcacccgtaa aatatgatcg caatcgattt cttagctttt tagctttgcc aacatatatc
    10261 acttgatcat tacgatcttt cattaaatag cagcctggtt ccataggtac gacatttaat
    10321 ttatttttaa ttcgttgctt atagtcttcc aaaaatatcc ccccttctaa ttttaaaatg
    10381 aatgatgcta caaaagtgct tcaaatttat caccactaat tctagttcat aatcagcatc
    10441 ttcgagtatg acttttcatg cacatcatgt aatattgaac attgttttat caaaccaact
    10501 gctacatgtc tatcagcatg cttgattatc atttaacgtt cttagattta ttaatcccac
    10561 cattctcaag tcgtaaacaa tatgatgact tatcattcaa ctttatatat taaaaacaca
    10621 aacaaaaact ggagcaattc attaaagaaa tgccccagtc gtcattggtt gtaacttata
    10681 aatgtttatc taaaacttca gctaagtttt cttttggttg gaaaccaaca actttatcaa
    10741 ctggttgacc gtctttaaag acgattaatg ttggaatact catcacttca tatttagctg
    10801 cagttgatgg attttcatca acatctaatt ttaaaatgtc agctttacct tcatagtcag
    10861 ctgctaattc ttctaatacc ggagcgatca ttttacaagg accacaccat gttgcccaaa
    10921 aatctactaa ttgtacacca gattctactt ttgaatcgaa atctgcatct gttactttaa
    10981 cgattgccat aaatgccaat cctccttaat tattattatg atgttcaaat tatatcagat
    11041 aacatgcttc atgtatttct atttgctcaa tttttatgct tatttcaaat aacatgtttg
    11101 aaatgtattc ttgttgcttt acattatttt aactgcagct aattaattta tcaaattata
    11161 atttatttta gtgttgcaac ggtaacgcca aatccacctt cacttggcat accaactcta
    11221 aagtcactaa cacttttatg ctttttcaaa tgttgttgta cacctttttg aagtgcacct
    11281 gtacctttac catgaatgat ataaacttgt tcgtaattac ttaaaacggc ttgatctaaa
    11341 tattgatcta gttcaattaa agcatcctca taacgatagc ctcgtaaatc aagttcagtt
    11401 ttaattgttt gacgattttg acgtgtaacc attttcgttg gcttaacttt ttctttttgt
    11461 tttttctcta aatcttcaat aggtaacttc attttaataa ttcccatttg aacaattgct
    11521 tcttcatcat tgacaatttc taaaacttca cccttttgac cgtaagataa tacttttact
    11581 tcatcaccag caacaatttt atcgtatttt tgcttttgta cattttgctt tatagatttc
    11641 gcttcataat gatcatctaa tcgtttcttc ttatcaatca attcatgttc tttaacatct
    11701 gcaccttttt gttcacgcaa ttgtcttagg tctttaataa tatcgtcagc ttcttttgtt
    11761 gctgctttaa ttttttgatt cgctttttct ttagcatctt caattagaga tttttcataa
    11821 ttttggaatt gttgatactg cttagataaa tcatcatgca cttgctccgc ttcttttaca
    11881 agacggtcca gttctaacct ctgtgtctct acacgtttgt aattacgctc taatgactca
    11941 atcatttcat ttatttcttt ttcatcagta ccaatcatcg tcttagcctt atttataatg
    12001 ttcaaactaa gacctaactt tttagaaata tcaaaagcat tggaacgacc tggtactccc
    12061 attaacaatt tataagttgg gcttaatgta tctacatcaa attctacact cgcattcata
    12121 acgccttctc gattataact atatgcttta agttcaggat agtgcgtcgt tgccattact
    12181 agagaaccaa tttttctaac atggtctaaa atgctcattg ctaaggcagc accttcactt
    12241 ggatctgtac ctgcacctaa ttcatcaaat aaaactaaac tatgtttgtc tgcacgcttt
    12301 aaaatttcaa ctatattcgt catatgagat gaaaaagttg ataatgattg ttctattgat
    12361 tgttcatctc cgatatcgca atatacattt ttaaatacac ttaactgact accatcaagt
    12421 gttggaatca ataatcctga ttgagccata acaataatta aacctaatgt ttttaatgtt
    12481 actgttttac cacctgtatt cggtcctgta ataattaccg tttcaatatc ttccataaat
    12541 tcgatggtat tagctacaac agtctcacga tttaataatg gatggtatgc tttaggtaaa
    12601 tatacagtac gttcctcttt aaatatcggc tttgttcctt taatacttct actatatctc
    12661 gcttttgcga ttaaaaaatc taactgaccc atgacttgtt ctgccacaag tagtgcatct
    12721 ttgtccgcag caacataacc agttagttgc gttaaaatgc gttctttttc aattgcttca
    12781 tcatgacgta atcgactaat ttgattattc atttcaacaa ctgatgatgg ctcaatatac
    12841 aatgtttgtc ctgaagcaga ttgatcatgt acaatcccat taaaatcttg tcgatattca
    12901 gctttgacag gtataacgtt tctttcattc ctaactgtta caatagcatc tgataatttt
    12961 ttctgatttg cttggctttt aacaatacgg tccaaatttt gtctaatacg ttgattcgtg
    13021 ctagaaattt tacttctaat cccttgcaat tcataactcg cattatcata taaatcatac
    13081 gtatcgcatg tttcatttat ttgttgaaaa agatcggtta acacaggtaa ttgattcatc
    13141 ttgtcatcta atattgggta tttaacacct tcatcttctt caaccaattg attataaaat
    13201 gtcttaaatt gattttgtac ttgaattaat ctttttatca agttaagctc tgatacattt
    13261 aaaacgccgc caatatcagc gcgatgaatg aatgctgata ctttagataa gccactcaag
    13321 cttggtaaac gatgcttatt atagatttga gcaatctcat ccgtttcttc catttgaaaa
    13381 acaaccgttt caaaattagt agctggcatc atttgattga ccttttccaa gcctaagtca
    13441 cttatagttt cattggcaac gagtgatttt attttttcaa attctaagac gtctaatgtt
    13501 ttttgtctca taaaatccct ctatttctta agttttatat tattttcaat aaagtcttta
    13561 aaagcttcac gcgataatgc gttaatcact ctatcttttg ttacaaatcc tttttgcgca
    13621 gttgctacac cataatgcat aaaatctaaa tgatttgtat gatgcgcatc agtgttaata
    13681 gttaatttca catttggata tttacgaacg atatcagcgc tcaaatccag tcgatgtgga
    13741 ttggcattaa tttctaatac tgtattcgtt tcttcagcta atgccattaa ttgttcaata
    13801 ttcggtttat aaccatctct tctacctata atacgccctg ttggatgcgc tatatgtcgc
    13861 acgtatggat tgcgacatgc attagctaat cgttccataa tttgttcttc tgattggtta
    13921 aagctttgat gaatagctcc aattacgtaa tcaagttgtg ctaaaatttc atcatcataa
    13981 tccagcgagc catcaggtaa tatatccatt tctgtacctg aataaatatc aatttcacta
    14041 tattctttat ctaaagcttt aatttcttcg ttttgtctta aaagtctttc tacttgtaag
    14101 ccattagcta cacgtaaact ttgtgaatga tcagtaatta ccatgaattt ataacctttt
    14161 gcgatatttg cttctaccat gtctcgaatg gaaaacgcac catcactaaa cgttgtatgc
    14221 atatgaatat caccattaat atcatctaat gtaatgatat tacttagatc tttatcaaat
    14281 tcgctaccat cttctctcat agcaggtggt ataaaattca cattaaaatg ttcatatatt
    14341 ttggcttcac tatcatattg aattaatgta ccatcagctt gttcaattcc atattcactt
    14401 actttttcat cacgtgcttt agcaagttgt cgaattctta tattatgttc ttttgaccca
    14461 gtaaaatgct gcaatgtatg ataaaaagca cttggttcaa ttaatcgaaa atcgacccca
    14521 atcgtttcat catcatacgc taattctaac gaaacttttg tgttccccac tgcaacttct
    14581 tttactttat tgggaatatt taataattgt tgctgcactg cttttgggtt atcggtgctt
    14641 attatgaaat ctaaatcttt gctcatttct ttaaaacgac ggaaacttcc tgcagatgaa
    14701 tattgatcga tataatttaa tgtatctata taatcaatga tttcttgatt aagtcttctc
    14761 atttgatcaa ttggatatct atctttctta gcaccaagtt gtttcacagc ttctaaaatg
    14821 ttttgttcgg ttttcttagc aaatccgctt aattcactaa cttttccatt ttcacaagca
    14881 acttgaagtg acgctttatc aacaatattc aactctttat atagcttagc aattttcttg
    14941 cttccaagtc cttgaatttt caaaagtgga ataagacctt ccggaacttc ttcctgtaat
    15001 tgctgtaaat actgagattc accggtctca cggtaatcat tgattacttc tgcaacacct
    15061 ttaccaatgc cttttaactc cgttacatca gatatttcat ctaatggtcg ttcatctaat
    15121 tcaagacttt gagctgcttt tcggtacgct gatattttaa aagtattttc cccttttaat
    15181 tccatataag tagcaatttg ttctaatagt ttgataacat ctttttttgt cataataaca
    15241 ctccataaaa agaagaccag gacgtatcat taatatatac ctttgtcctg acctcttatg
    15301 ataatttata aattaatggt gaaagccgat aaatatggga tgtgaaaaat caatgaatga
    15361 ctcacaatag atattttaag ctgatgttgt aatgcttcgt tgggatataa tgccaataga
    15421 tataacgtaa agtgtaagac aattatcgtc ataaacacac caactatcat tcccattgca
    15481 cgactgaaaa tatgaatgtt ttgatacgct attattttat caaaagttac gataattaga
    15541 tataaaatga acttacaaaa caatgtaatc attaaaaaag ctacaatcgc ttcaaatcga
    15601 tgttgtagat gattaaaatg aaacgcaaaa gttgtattaa atgctgttgt tttaggatat
    15661 ggaataaata caattaatct ttctacaata gatttgtaaa attgactggc aatccacaat
    15721 gatacaatcg ttgcactcaa atgtatcata gataaccaaa aacctcgtct gaatccaacg
    15781 atgacaaaat acacaaagaa aatgattata ataaaatcaa tgatcattta ttgctcacgc
    15841 tgctgcaatt tgtgaatttg ttgtttcaaa cgtcgatttt cttcttctag tagtactttt
    15901 tcatgcataa tattcacagc agttagtatt gcttttcttg aagtatctaa acctgctgct
    15961 ttatacccta attcttttat tttatcatca actaaatgtg ctacatatcg tatgtgctct
    16021 gggttatctt ccccaacaat tgtaaaaagc tgatcattaa ttgatacatt taccttgttt
    16081 ttaaactgtg ccatttataa tttctcctga tccttttttt aaaatctaaa ttcacgttat
    16141 aaaatatgac tgaatagttt gtctgaattt gatactaata ttgttatatt gcaattatga
    16201 taaaacaaca acacaatctc tatagatgac ttaatgttct ttttataatg aaatgaaatt
    16261 atgtaaagaa ttttctattc aatactttat catgtttaaa ttgtgtcact ataacatt
//
LOCUS       DAPIDM010000048        16262 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000048 DAPIDM010000000
VERSION     DAPIDM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.048"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            63..1400
                     /locus_tag="RJE42_001353"
     CDS             63..1400
                     /locus_tag="RJE42_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDV5876779.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLDYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRENIIEYLSYFQTSSPSYLIMASLESAAQFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1402..2019
                     /locus_tag="RJE42_001354"
     CDS             1402..2019
                     /locus_tag="RJE42_001354"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV5876780.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2047..2376
                     /locus_tag="RJE42_001355"
     CDS             2047..2376
                     /locus_tag="RJE42_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDV5876781.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2590..3516
                     /locus_tag="RJE42_001356"
     CDS             2590..3516
                     /locus_tag="RJE42_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV5876782.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNALALNEEFDLLALRKSVIRWCELLLTNKSMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3517..4320
                     /locus_tag="RJE42_001357"
     CDS             3517..4320
                     /locus_tag="RJE42_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDV5876783.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4337..4684
                     /gene="yabA"
                     /locus_tag="RJE42_001358"
     CDS             4337..4684
                     /gene="yabA"
                     /locus_tag="RJE42_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDV5876784.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4958..5683
                     /locus_tag="RJE42_001359"
     CDS             4958..5683
                     /locus_tag="RJE42_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDV5876785.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGYFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5676..5924
                     /locus_tag="RJE42_001360"
     CDS             5676..5924
                     /locus_tag="RJE42_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDV5876786.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5926..6765
                     /gene="rsmI"
                     /locus_tag="RJE42_001361"
     CDS             5926..6765
                     /gene="rsmI"
                     /locus_tag="RJE42_001361"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV5876787.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDILKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAARDA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIN"
     gene            7050..9023
                     /gene="metG"
                     /locus_tag="RJE42_001362"
     CDS             7050..9023
                     /gene="metG"
                     /locus_tag="RJE42_001362"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV5876788.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     QVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMINKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTEP
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAIVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9054..9827
                     /locus_tag="RJE42_001363"
     CDS             9054..9827
                     /locus_tag="RJE42_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDV5876789.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            9994..10530
                     /gene="rnmV"
                     /locus_tag="RJE42_001364"
     CDS             9994..10530
                     /gene="rnmV"
                     /locus_tag="RJE42_001364"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDV5876790.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10541..11434
                     /gene="rsmA"
                     /locus_tag="RJE42_001365"
     CDS             10541..11434
                     /gene="rsmA"
                     /locus_tag="RJE42_001365"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV5876791.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11534..11797
                     /locus_tag="RJE42_001366"
     CDS             11534..11797
                     /locus_tag="RJE42_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDV5876792.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12108..12956
                     /locus_tag="RJE42_001367"
     CDS             12108..12956
                     /locus_tag="RJE42_001367"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV5876793.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFNIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRNYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            12970..13794
                     /gene="purR"
                     /locus_tag="RJE42_001368"
     CDS             12970..13794
                     /gene="purR"
                     /locus_tag="RJE42_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDV5876794.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13811..14191
                     /locus_tag="RJE42_001369"
     CDS             13811..14191
                     /locus_tag="RJE42_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV5876795.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKIVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14263..14565
                     /gene="spoVG"
                     /locus_tag="RJE42_001370"
     CDS             14263..14565
                     /gene="spoVG"
                     /locus_tag="RJE42_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDV5876796.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            14754..14884
                     /locus_tag="RJE42_001371"
                     /pseudo
     CDS             14754..14884
                     /locus_tag="RJE42_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14907..16259
                     /gene="glmU"
                     /locus_tag="RJE42_001372"
     CDS             14907..16259
                     /gene="glmU"
                     /locus_tag="RJE42_001372"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV5876797.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQHPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTFIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTVGDGVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
BASE COUNT         5902 a         2170 c         3279 g         4911 t
ORIGIN      
        1 tataattaat acttttgaaa gtaactaatt ccaaaacatt gtaaataaag gaagcgtata
       61 tcatgaagca acctatttta aataaattag aaagtttaaa tcaagaagaa gcgatttctt
      121 tgcatgttcc gggtcataaa aatatgacta tcggtcattt atctcaatta tcaatgacaa
      181 tggataaaac tgaaatacct ggattagatg atttacatca tcctgaagaa gtcattttgg
      241 aaagtatgaa gcaggtggag aaacattcag attatgatgc ttatttctta gtgaatggca
      301 ccacttcagg aatattatct gtcatccagt ctttttcaca gaaaaaaggc gatatcttaa
      361 tggcaagaaa tgtacataaa tctgtattac atgcgctcga tattagccaa caagaagggc
      421 attttattga aacgcatcaa agtccgttaa cgaatcatta taataaagtt aatttaagcc
      481 gtttgaataa tgacggtcac aaacttgctg tgttgactta tcctaactat tacggtgaaa
      541 catttaatgt agaagaggtt atcaaatctt tgcaccaatt aaatatccct gtactcattg
      601 acgaagcaca cggcgcgcac tttggattgc aaggatttcc agattctaca ttagattatc
      661 aagctgacta tgttgttcaa tcttttcata aaacgttacc tgctttaacg atgggctcgg
      721 tactttatat tcataaaaat gcaccttata gagaaaatat tatagaatat ctaagctact
      781 tccaaacatc tagtccttcg tatttgatta tggctagttt agagtcagct gcccagttct
      841 ataaaacata tgatagtacc gtgttttttg ataagagagc gcaattaatc gaatgtttgg
      901 agaagaaggg ttttgaaatg cttcaagttg atgatccgtt gaagctgttg ataaaatatg
      961 aaggctttac aggtcatgat attcaaaatt ggtttatgaa tgcacatatc tatttagaat
     1021 tagcggacga ctatcaagca ttagcgatat tgccgttatg gcatcatgat gatacgtatt
     1081 tatttgattc gcttttacgt aaaattgaag atatgatttt accgaaaaaa tcagtttcta
     1141 aagttaaaca aacacaactt ttaacaactg aaggtaacta taaaccaaaa cgctttgaat
     1201 atgttacttg gtgtgatttg aaaaaggcaa aaggtaaagt tctggcgcga catattgtcc
     1261 cgtatccgcc agggattcct attattttca aaggagaaac aataactgaa aatatgatag
     1321 aattggtaaa tgaatatctg gaaactggaa tgatagttga aggaattaaa aataataaaa
     1381 ttttagttga ggatgaataa aatgtcagct tttataactt ttgagggccc agaaggctca
     1441 ggtaaaacaa ctgtaattaa tgaagtttat catagattag taaaagatta tgatgtcatt
     1501 atgacaagag aaccgggcgg cgttcctact ggtgaagaaa tacgtaaaat tgtattagaa
     1561 ggcaatgata tggacattag aactgaagca atgttatttg ctgcatctag aagagaacat
     1621 cttgtattaa aggtcatacc agctttaaaa gaaggtaagg ttgtgttgtg tgatcgctat
     1681 atcgatagtt cattagctta tcaaggttat gctagaggga ttggcgttga agaagtaaga
     1741 gcattaaacg aatttgcaat aaatggatta tatccagact tgacgattta tttaaatgtt
     1801 agtgctgaag taggtcgcga acgtattatt aaaaattcaa gagatcaaaa tagattagat
     1861 caagaagatt taaagtttca cgaaaaagta attgaaggtt accaagaaat cattcataat
     1921 gaatcacaac ggttcaaaag cgttaatgca gatcaacctc ttgaaaatgt tgttgaagac
     1981 acgtatcaaa ctatcatcaa atatttagaa aagatatgat ataattgtta gaagaggtgt
     2041 tataaaatga aaatgattat agcgatcgta caagatcaag atagtcagga acttgcagat
     2101 caacttgtta aaaataactt tagagcaaca aaattggcaa caacaggtgg gtttttaaga
     2161 gcaggtaata caacattctt atgtggtgtc aatgatgacc gtgtagatga aatattgtct
     2221 gtgattaatc aaacgtgtgg taatagagaa cagttggttt cacctattac acctatggga
     2281 ggcagtgcgg attcgtacat tccatatcca gttgaagttg aagttggcgg tgctactgta
     2341 tttgttatgc cagttgatgc attccatcaa ttttaattct ataatacaat catcaattag
     2401 agatacttaa aatagtgtat taataagtta ttaacaattt tgggttgctt gactgcgact
     2461 agttcagatg ccaatagatt tgatttttgt ggttctaaga ataatcacaa atcatggtcg
     2521 ctattgttgc agtaattagt tgctctttgg caaccttttt atataaaagc aaaagggagt
     2581 ttgtaatgaa tggatgaaca gcaacaattg acgaatgcat atcattcaaa taaattatcg
     2641 catgcctatt tatttgaagg tgatgatgca caaacgatga aacaagttgc gattaatttt
     2701 gcaaagctta ttttatgtca aacagatagt caatgtgaaa caaaggttag tacatataat
     2761 catccagact ttatgtatat atcaacaact gagaatgcaa ttaagaaaga acaagttgaa
     2821 caacttgtgc gtcatatgaa tcaacttcct atagaaagca caaataaagt gtacatcatt
     2881 gaagactttg aaaagttaac tgttcaaggg gaaaacagta tcttgaaatt tcttgaagaa
     2941 ccaccggaca atacgattgc tattttattg tctacaaaac ctgagcaaat tttagacaca
     3001 atccattcaa ggtgtcagca tgtatatttc aagcctattg ataaagaaaa gtttataaat
     3061 agattagttg aacaagacat gtctaagcca gtagctgaaa tgattagtac ttatactacg
     3121 caaatagata atgcactggc tttaaatgaa gaatttgatt tattagcatt aaggaaatca
     3181 gttatacgtt ggtgtgaatt gttgcttact aataagtcaa tggcacttat aggtattatt
     3241 gatttattga aacaggctaa aaataaaaaa ctgcaatctt taactattgc agctgtgaat
     3301 ggtttcttcg aagatatcat acatacaaag gtaaatgtag aggataaaca aatatatagt
     3361 gatttaaaaa atgatattga tcaatatgcg caaaagttgt cgtttaatca attaattttg
     3421 atgtttgatc aactgacgga agcacataag aaattgaatc aaaatgtaaa tccaacgctt
     3481 gtatttgaac aaatcgtaat taagggtgtg agttagatgc caaatgtaat aggtgttcag
     3541 tttcaaaaag cgggaaaatt agaatattat acacctaatg atatacaagt agatatagat
     3601 gactgggtag ttgtcgaatc taaaagaggc atagagatag gtattgttaa aaatccatta
     3661 atggatattg ctgaagagga tgttgtgtta cctcttaaaa atattattcg cattgctgat
     3721 gacaaagata ttgataaatt taattgtaat gaacgagatg ctgaaaatgc attaatacta
     3781 tgtaaagaca ttgtaagaga acaaggtttg gacatgcgtt tagtcaattg cgaatataca
     3841 ttagataaat cgaaagttat ttttaatttt acggcggatg atcgtattga ttttagaaaa
     3901 ttagtaaaaa tattagcgca acatttaaaa acacgtatcg aattgagaca aattggtgta
     3961 agggatgaag ccaaattgct tggcggtatc ggaccttgtg gtaggtcgtt atgttgttct
     4021 acatttttag gagattttga accagtatcg attaagatgg ctaaggatca aaatttatca
     4081 ttaaatccaa ctaaaatttc cggtgcatgt ggtcgtttga tgtgttgttt aaaatatgaa
     4141 aatgactact atgaggaagt acgtgcgcaa ttacctgata tcggtgaagc aattgaaacg
     4201 cctgatggta acgggaaagt agttgcttta aatatattag acatttctat gcaggtgaag
     4261 cttgagggac atgaacagcc acttgaatat aaattagaag aaatagaaac tatgcattaa
     4321 ggaggcatta ttacatttgg atcgcaatga aatatttgaa aaaataatgc gtttagaaat
     4381 gaatgtcaat caactttcaa aggaaacttc agaattaaag gcacttgcag ttgaattagt
     4441 agaagaaaat gtagcgcttc aacttgaaaa tgataatttg aaaaaggtgt tgggcaatga
     4501 tgaaccaact actattgata ctgcgaattc aaaaccagca aaagctgtga aaaagccatt
     4561 accaagtaaa gataatttgg ctatattgta tggagaagga tttcatattt gtaaaggcga
     4621 attatttgga aaacatcgac atggtgaaga ttgtctgttc tgtttagaag ttttaagtga
     4681 ttaatcaagc acactcaaat agtgttataa ttataaatga atatggtttg gataagtctg
     4741 agacaatgca tgtttcaggc tttaattgtg tataaagttt tggtgattgc ataagagata
     4801 gcggtactaa atgttattat taagtgtgca cgcagtatca ttagttataa aatgtagctg
     4861 ttaaaagcca aaaatacatc gaatgtagat aggcatataa tataaaaaga gatttcaatt
     4921 actcaataga aaaaggttgt cttcatagga gttaaaaatg ttaaaagaga atgaacgatt
     4981 tgatcaacta atcaaagaag attttagtat tattcaaaat gatgatgttt tttcgttttc
     5041 aacggatgct ttgttgttag ggtattttac aaaacctaga acaaaagata ttgtgttgga
     5101 cttatgttca ggcaatgggg tgataccctt gttattgttt gcgaaacatc cacgacatat
     5161 agaaggtgtt gagattcaaa aaacacttgt cgatatggcg cgacgcacat ttcaattcaa
     5221 tgatgttgat gaatatctaa caatgcatca catggatttg aaaaacgtta ctaaagtatt
     5281 taaaccttca caatatactt tagtaacgtg taatccgcct tattttaaag agaatcagca
     5341 acaccaacat caaaaagaag cacataagat agcgagacat gagattatgt gtacacttga
     5401 agattgcatg attgcagccc gtcatttatt aaaagagggt ggcaggctaa acatggtaca
     5461 tcgtgcagat agactaatgg atgtcttgtt tgaaatgaga aaagtgaata ttgaacctaa
     5521 gaaagtcgtt tttatatata gtaaagtagg gaaatcggca caaacgatag ttgtagaagg
     5581 tcgaaaaggt ggaaatcaag gtttagaaat catgccccca ttttatattt ataatgaaga
     5641 tggtaattat agcgaagaaa tgaaggaagt atattatgga tagtcatttt gtatatattg
     5701 taaaatgtag tgatggaagt ttatatacag gatacgctaa agacgttaat gcacgtgttg
     5761 aaaaacataa ccgaggtcaa ggagccaaat atacgaaagt aagacgtccg gtgcatttag
     5821 tttatcaaga aatgtatgag acaaagtctg aagcattgaa gcgtgaatat gaaattaaaa
     5881 cttataccag acaaaagaaa ttgcgattaa ttaaggagcg atagtatggc tgtattatat
     5941 ttagtgggca caccaattgg taatttagca gatattactt atagagcagt tgatatattg
     6001 aaacgtgttg atatgattgc ttgtgaagac actagagtaa ctagtaagct gtgtaatcat
     6061 tatgatattc caactccatt aaagtcatat cacgaacata acaaggataa gcagactgct
     6121 tttatcattg aacagttaga attaggtctt gacgttgcgc tcgtatctga tgctggattg
     6181 cccttaatta gtgatcctgg atacgaatta gtagtggcag ccagagacgc taatattaaa
     6241 gtagagactg tgcctggacc taatgctggg ctgacggctt tgatggccag tggattacct
     6301 tcatatgtat atacattttt aggatttttg ccacgaaaag agaaagaaaa aagtgctgta
     6361 ttagagcaac gtatgcatga aaatagcaca ttaattatat acgaatcacc gcatcgtgtg
     6421 acagatacat taaaaacaat tgcaaaaata gatgcaacac gacaagtatc actagggcgt
     6481 gaattgacta agaagtttga acaaattgta actgatgatg taacacaatt acaagcattg
     6541 attcagcaag gcgatgtacc attgaaaggc gaatttgtta tcttgattga gggtgctaaa
     6601 gcgaacaatg agatatcgtg gtttgatgat ttatctatca atgagcatgt tgatcattat
     6661 attcaaactt cacagatgaa accaaaacaa gctattaaaa aagttgctga agaacgacaa
     6721 cttaaaacga atgaagtata taatatttac catcaaataa attaatcact ttatcgatta
     6781 tatgaaattt taaacgattt tataaacgca agctgtaatt ttaaatggta agttatcatt
     6841 ttgcattgat actgataaaa tgatgttgac tatgataaaa aaatgatgac atcgacgttt
     6901 tttaatgtaa aataaataca ttgaaagtaa taaatacctt aacattgaat aagatgaaaa
     6961 tgagatgacg agataaatgt tcgcgtccgt tgaaatgcat agaaatctta gatattattt
     7021 gaagtgagac attacgagga ggaacagtta tggctaaaga aacattttat ataacaaccc
     7081 caatatacta tcctagtggg aatttacata taggacatgc atattctaca gtggctggag
     7141 atgttattgc aagatataag agaatgcaag gatatgatgt tcgttatttg actggaacgg
     7201 atgaacacgg tcaaaaaatt caagaaaaag ctcaaaaagc tggtaagaca gaaattgaat
     7261 atttggatga gatgattgct ggaattaaac aattgtgggc taagcttgaa atttcaaatg
     7321 atgattttat cagaacaact gaagaacgtc ataaacatgt cgttgagcaa gtatttgaac
     7381 gtttattaaa gcaaggtgat atctatttag gtgaatatga aggttggtat tctgttccgg
     7441 atgaaacata ctatacagag tcacaattag tagacccaca atacgaaaac ggtaaaatta
     7501 ttggtggtaa aagtccagat tctggacacc aagttgaact agtaaaagaa gaaagttatt
     7561 tctttaatat tagtaaatat acagaccgtt tattagagtt ctatgaccaa aatccagatt
     7621 ttatacaacc accatcaaga aaaaatgaaa tgattaacaa cttcattaaa ccaggacttg
     7681 ctgatttggc tgtttctcgt acatcattta actggggtgt ccatgttccg tctaatccaa
     7741 aacatgttgt ttatgtttgg attgatgcgt tagttaacta tatttcagca ttaggctatt
     7801 tatcagatga tgagtcacta tttaacaaat actggccagc agatattcat ttaatggcta
     7861 aggaaattgt gcgattccac tcaattattt ggcctatttt attgatggca ttagacttac
     7921 cgttacctaa aaaagtcttt gcacatggtt ggattttgat gaaagatgga aaaatgagta
     7981 aatctaaagg taatgtcgta gaccctaata ttttaattga tcgctatggt ttagatgcca
     8041 cacgttatta tctaatgcgt gaattaccat ttggttcaga tggcgtattt acacctgaag
     8101 catttgttga gcgtacaaat ttcgatctag caaatgactt aggtaactta gtaaaccgta
     8161 cgatttctat gattaataag tactttgatg gcgaattacc agcgtatcaa ggtccacttc
     8221 atgaattaga tgaagaaatg gaagctatgg ctttagaaac agtgaaaagc tatactgaaa
     8281 gcatggaaag tttgcaattt tctgtggcat tatctacggt atggaagttt attagtagaa
     8341 cgaataagta tattgacgaa acaacgcctt gggtattagc taaggacgat agccaaaaag
     8401 atatgttagg caatgttatg gctcacttag ttgaaaatat tcgttatgca gctgtattat
     8461 tacgtccatt cttaactcat gcgccgaaag agatttttga acaattgaac attaacaatc
     8521 ctcaatttat ggaatttagt agtttagagc aatatggtgt gcttactgag ccaattatgg
     8581 ttactgggca acctaaacct attttcccaa gattggatag cgaagcggaa attgcatata
     8641 tcaaagaatc aatgcaaccg cctgctactg aagaggaaaa agaagagatt cctagcaaac
     8701 ctcaaattga tattaaagac tttgataaag ttgaaattaa ggcagcaacg attattgatg
     8761 ctgaacatgt taagaagtca gataagcttt taaaaattca agtagactta gattctgaac
     8821 aaagacaaat cgtatcagga attgccaaat tctatacacc agatgatatt attggtaaaa
     8881 aagtagcaat tgttactaac ttgaaaccag ctaaattaat gggacaaaaa tctgaaggta
     8941 tgatattatc tgctgaaaaa gatggtgtat taaccttagt aagtttacca agtgcaattc
     9001 caaatggtgc agtgattaaa taactgtatt tttaaaaatt aggagagata attatgttaa
     9061 tcgatacaca tgtccattta aatgatgagc aatacgatga tgatttgagt gaagtgatta
     9121 cacgtgctag agaagcaggt gttgatcgta tgtttgtagt tggttttaac aaatcgacaa
     9181 ttgaacgcgc gatgaaatta atcgatgagt atgatttttt atatggcatt atcggttggc
     9241 atccagttga cgcaattgat tttacagaag aacacttgga gtggattgaa tctttagctc
     9301 agcatccaaa agtgattggt attggtgaaa tgggattaga ttatcactgg gataaatctc
     9361 ctgcagatgt tcaaaaggaa gtttttagaa agcaaattgc tttagctaag cgtttgaagt
     9421 taccaattat cattcataac cgtgaagcaa cacaagactg tatcgatatc ttattggagg
     9481 agcatgctga agaggtaggc gggattatgc atagctttag tggttctcca gaaattgcag
     9541 atattgtaac taataagctg aatttttata tttcattagg tggaccagtg acatttaaaa
     9601 atgctaaaca gcctaaagaa gttgctaagc atgtgtcaat ggagcgattg cttgttgaaa
     9661 ccgatgcacc gtatctttcg ccacatccat atagagggaa gcgaaatgaa ccggcgagag
     9721 taactttagt agctgaacaa attgctgaat taaaaggctt atcttatgaa gaagtgtgcg
     9781 aacaaacaac taaaaatgca gagaaattgt ttaatttaaa ttcataaagt taaaagtgag
     9841 aaagatcacc gccataaatg taaacgatgc tatattcgtt taatatgcta tggttctttc
     9901 tcactttttt aaattaaaat atcgtgcatg tgcaatacgt gcgatagaga tggttagagc
     9961 tttgaaatta agaattgtag gaaggcgttt taaatgaaaa tcaatgagtt tatagttgta
    10021 gaaggacgag atgatactga gcgtgttaaa cgagctgttg aatgtgatac gattgaaacg
    10081 aatggtagtg ccatcaacga acaaacttta gaagtaatta gaaatgctca acaaagtcga
    10141 ggcgttattg tattaacaga tccagatttc ccaggagata aaattagaag tacaattact
    10201 gaacatgtca aaggtgttaa acatgcgtat attgatagag aaaaagctaa aaataaaaaa
    10261 gggaaaattg gtgttgaaca tgccgactta attgatatta aagaagcgtt aatgcatgtt
    10321 agttcaccct ttgatgaagc ttatgaatca attgataaat ctgtgctaat agagttgggg
    10381 ttaatcgttg ggaaagatgc aaggcgccgt agagaaattt taagtagaaa attgcgaatc
    10441 ggccattcca atggtaagca gttattgaaa aagttaaatg catttggtta taccgaagcg
    10501 gatgtaaggc aagctttaga agatgaatga ggaagtgaaa atgttggata ataaagatat
    10561 tgcaacacca tcaagaacgc gagcgttgtt agataaatat ggctttaatt ttaaaaaaag
    10621 tttaggacag aactttttaa tagatgtgaa tatcattaat aatatcattg atgcaagtga
    10681 tattgatgca caaactgggg tgattgaaat tggtccaggt atggggtcgt tgacagaaca
    10741 attggccaga catgctaaaa gagtattggc atttgaaatt gatcaacgtt taatacctgt
    10801 attaaatgat acactatcac cttatgataa tgtgacggtg attaatgaag atattttaaa
    10861 agcgaatatt aaagaagctg ttgaaaatca tttacaagat tgcgaaaaaa taatggttgt
    10921 tgcaaacctg ccgtactata ttacgacgcc aattttatta aatttaatgc aacaagatat
    10981 accaattgat ggctacgtgg tgatgatgca aaaagaagtg ggcgaacgct taaatgctga
    11041 agtaggttca aaagcatatg gttcattatc aattgtcgta caatactata cagagactag
    11101 taaagtatta acggtaccta aatctgtatt tatgccacca cctaatgttg attcaatagt
    11161 tgtaaaactg atgcagagaa ctgaaccgtt agtaacagta gataacgagg aagcattttt
    11221 taagttagca aaagcagcat ttgcacaaag aagaaagaca attaacaata actatcaaaa
    11281 ttattttaaa gatggtaaac aacacaaaga agtgatttta caatggttgg aacaagcggg
    11341 tattgatcca agacgtcgcg gtgaaacgct atctattcaa gattttgcta aattgtatga
    11401 agaaaagaaa aaattccctc aattagaaaa ttaaatgatt gacaaagcaa agcactattg
    11461 ttaaaattta aattttgttt gacgaaaacg ctgcaaatat ggtattatgt aacttgtagc
    11521 gaggtggagc aatatgccaa aatcaatttt ggacatcaaa aattctattg attgtcatgt
    11581 aggaaatcgt attgtactga aagccaatgg aggccgtaag aaaacaataa aacgttctgg
    11641 aattttaaaa gaaacatatc cgtcagtttt cattgttgag ttagatcaag acaaacacaa
    11701 ctttgagaga gtatcttata catacactga tgtgttaact gaaaatgttc aagtttcatt
    11761 tgaagaggat aatcatcacg aatcaattgc acactaaata agacatatag agatgttaga
    11821 cgtttcttag tataagaagt aaatattatg ataattattt gagtgttggg cattatgttc
    11881 aatactcttt ttatttacaa aatgtttaac actgatgttt cgcttataga tttttcagta
    11941 aatggataat tgtatttata aacacaaata caagtaaata ctaagtaatt agatggagaa
    12001 aattactttt ttattaaaaa aacactaaaa aacaaattaa aatgtcaaat attaattctc
    12061 tttatgttaa aatcatcata ttaagataac gaaaagaggg cgcaaaaatg atatatgaaa
    12121 cggcaccagc caaaattaat tttacgctcg atacactttt taaaagaaat gatggctatc
    12181 atgagattga aatgataatg acaacagttg atttaaatga tcgtttaact ttccataaaa
    12241 gaaaagatcg aaagatagtt gttgagattg aacataatta tgtgccttct aatcataaaa
    12301 atctcgcata tcgtgcagcg caactattta ttgagcaata tcaactaaag caaggtgtaa
    12361 caatttctat cgataaagaa atacctgttt ctgctggctt agctggaggt tcggctgatg
    12421 cagcagcaac gttgagagga ttgaatcgac tttttaatat aggggcgagt ttggaagaat
    12481 tggctctact aggcagtaaa atcgggacag atattccgtt ttgtatttat aataaaactg
    12541 cactatgtac tggaagagga gagaaaattg agtttttaaa taaaccacct tcagcttggg
    12601 tgattcttgc taaaccaaac ttaggcatat catcaccaga tatatttaag ttgatcaatt
    12661 tagataagcg ttacgacgta catacgaaaa tgtgttatga ggccttagaa aatcggaatt
    12721 atcaacaatt atgtcaaagt ttgtctaatc gattagagcc aatttctgtt tcaaaacacc
    12781 cacaaatcga taaattaaaa aacaatatgt tgaaaagtgg tgcagatggt gcgttaatga
    12841 gtggaagtgg accgactgtg tatgggctag cacgaaaaga aagccaagca aaaaatattt
    12901 ataatgcagt taacggttgt tgtaatgaag tgtacttagt tagactatta ggatagaagg
    12961 gttgaaaaga tgagatataa acgaagcgag agaattgttt ttatgacgca atatttgatg
    13021 aaccatccga ataaattgat tccattaact ttttttgtga aaaaatttaa acaggcgaag
    13081 tcttcaataa gtgaagatgt ccaaattata aaaaatacat tccaaaaaga aaagttaggt
    13141 acagtaatta ctactgctgg cgcaagtggt ggtgttacgt ataaaccaat gatgagtaaa
    13201 gaagaggcga ctgaagttgt taatgaggtc attactctat tagaagagaa agaacgtttg
    13261 ttacctggcg ggtatttatt tttatcagat ttggtaggta atccatcgct actaaacaaa
    13321 gttggtaagt taattgccag tatttacatg gaagaaaaat tagatgctgt tgttaccatt
    13381 gcgacaaaag gtatttcatt ggcaaatgcg gttgctaata ttttaaattt accagtagta
    13441 gtgattagaa aagacaacaa ggtgactgaa ggttctacag tttcaattaa ttacgtttca
    13501 ggatcttcaa gaaaaataga gacaatggta ctttcgaaga gaactttagc agaaaattca
    13561 aatgttttag ttgtcgatga ttttatgagg gctggtggct ctattaatgg tgttatgaat
    13621 ttaatgaatg agtttaaagc ccatgtaaaa ggggtatcag tacttgtaga atcaaaagaa
    13681 gttaaacaaa gattgattga agattatact tccttagtga aattatctga tgtagatgaa
    13741 tataatcaag agtttaacgt agaacctggc aacagtttat ctaagttttc ataaaaggag
    13801 ttttagtatt atgaaaatca ttaacacaac aagattaccg gaagcacttg gaccatattc
    13861 gcatgcaaca gttgtaaatg gtatggttta tacttctggt cagattccat tgaatgttga
    13921 tgggaaaatc gtaagcgctg atgttcaagc acagacaaaa caagttttag aaaatttaaa
    13981 gattgttttg gaagaagcag gatctgattt gaattctgtt gcgaaagcga ccattttcat
    14041 taaagatatg aatgatttcc aaaaaataaa tgaagtgtat ggtcaatatt ttaatgaaca
    14101 caagccagcg cgtagttgtg tagaggttgc gcgtttgcca aaagatgtga aagtagaaat
    14161 tgaattagta agtaaaatta aggaattata attttcgatt aatatgttta atcaagcttc
    14221 taaataaaac agagagatat atactatagg ggggctcact acatgaaagt gacagatgta
    14281 agacttagaa aaatacaaac agatggacga atgaaagcac tcgtttccat tacattagat
    14341 gaagctttcg taattcatga tttacgtgta attgaaggaa actctggctt gttcgttgca
    14401 atgccaagta aacgtacacc agatggtgaa ttccgcgaca tcgcgcatcc tattaattca
    14461 gatatgagac aagaaattca agatgcagtg atgaaagtat atgatgaaac agatgaagta
    14521 gtaccagata aaaacgctac atcagaagat tcagaagaag cttaatcaat tttatattta
    14581 gcgatgtaat acatttgcaa taagttgatt tgatactgtc gataaagcat aaagctttgt
    14641 cggcagtttt tttagtttgt attaatgtta attttatgaa atgaaaggct aataaatatt
    14701 tatgttaaca gattatgatg atatgaaaat tattgataca ttgctgataa aaattgcgtt
    14761 tgaatgatgc tcgtattttt gaagtaagaa aaagttgttt ttaaaattac aacgaattaa
    14821 aaacaatgcc ttttatatgt tgaaagagta ttgcagatta aattataata atgacgaagt
    14881 gtaaaattta atgggggtta atgttcatgc gaagacacgc gataattttg gcagcaggta
    14941 aaggcacaag aatgaaatct aaaaagtata aagtgctaca cgaggttgct gggaaaccta
    15001 tggtcgaaca tgtattggaa agtgtgaaag gctctggtgt cgatcaagtt gtaaccatcg
    15061 taggacatgg tgctgaaagt gtaaaaggac atttaggcga gcgttcttta tacagttttc
    15121 aagaggaaca actcggtact gcgcatgcag tgcaaatggc gaaatcacac ttagaagaca
    15181 aggaaggtac gacgattgtt gtatgtggtg atacaccgct catcacaaag gaaacattag
    15241 aaacattgat tgcgcatcat gaggatgcta atgctcaagc aacagtatta tctgcatcga
    15301 ttcaacatcc atatggatac ggaagaatcg ttcgaaatgc gtcaggtcgt ttagaacgca
    15361 tagttgaaga gaaagatgca acgcaagctg aaaaggatat taatgaaatt agttcaggta
    15421 tttttgcgtt taataataaa acgttgtttg aaaaattaac acaagtgaaa aatgataatg
    15481 cgcaaggtga atattacctc cctgatgtat tgtcgttaat tttaaatgat ggcggcatcg
    15541 tagaagtcta tcgtaccaat gatgttgaag aaatcatggg tgtaaatgat cgtgtaatgc
    15601 ttagtcaggc tgagaaggcg atgcaacgtc gtacgaatca ttatcacatg ctaaatggtg
    15661 tgacaatcat cgatcctgac agcactttta ttggtccaga cgttacaatt ggcagtgata
    15721 cagtcattga accaggcgta cgaattaatg gtcgtacaga aattggcgaa gatgttgtta
    15781 ttggtcagta ctctgaaatt aacaatagta cgattgaaaa tggtgcatgt attcaacagt
    15841 ctgttgttaa cgatgctagc gtaggagcga atactaaggt cggaccgttt gcgcaattga
    15901 gaccaggcgc gcaattaggt gcagatgtta aggttggaaa ttttgtagaa attaaaaaag
    15961 cagatcttaa agatggtgca aaggtttcgc atttaagtta tattggcgat gctgtaatag
    16021 gcgaacgtac gaatattggc tgtggaacga taacagttaa ctatgatggt gaaaataaat
    16081 ttaaaactat cgtcggcaaa gattcatttg tcggatgcaa tgttaattta gtagcgccag
    16141 taacggttgg tgatggtgta ttggtggcag ctggttccac aatcacagat gacgtaccaa
    16201 atgacagttt agctgtggca agagcaagac aaacaacaaa agaaggatat aggaaataat
    16261 ca
//
LOCUS       DAPIDM010000049        15830 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000049 DAPIDM010000000
VERSION     DAPIDM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.049"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..264
                     /gene="sarX"
                     /locus_tag="RJE42_001373"
     CDS             <1..264
                     /gene="sarX"
                     /locus_tag="RJE42_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDV5876798.1"
                     /translation="DLAVLDLTMKYCKDEKVLMQSFLKTAMDELDLSRTKLLVSIRRL
                     IEKERLSKVRSSKDERKIYIYLNDDDISKFNALFEDVEQFLNI"
     gene            454..1170
                     /locus_tag="RJE42_001374"
     CDS             454..1170
                     /locus_tag="RJE42_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV5876799.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIEKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDDILETLMEQDVDVND
                     VFDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            1170..1643
                     /locus_tag="RJE42_001375"
     CDS             1170..1643
                     /locus_tag="RJE42_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV5876800.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1995..2639
                     /locus_tag="RJE42_001376"
     CDS             1995..2639
                     /locus_tag="RJE42_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDV5876801.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKIHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2756..3622
                     /locus_tag="RJE42_001377"
     CDS             2756..3622
                     /locus_tag="RJE42_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5876802.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMTSFVEEPKA"
     gene            3980..5200
                     /locus_tag="RJE42_001378"
     CDS             3980..5200
                     /locus_tag="RJE42_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV5876803.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSSASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            5197..5685
                     /locus_tag="RJE42_001379"
     CDS             5197..5685
                     /locus_tag="RJE42_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDV5876804.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWIGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5784..6473)
                     /locus_tag="RJE42_001380"
     CDS             complement(5784..6473)
                     /locus_tag="RJE42_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDV5876805.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6600..7043
                     /locus_tag="RJE42_001381"
     CDS             6600..7043
                     /locus_tag="RJE42_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876806.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            7110..7493
                     /locus_tag="RJE42_001382"
     CDS             7110..7493
                     /locus_tag="RJE42_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876807.1"
                     /translation="MKKLIISIMSVMILLAGCGKSEEKATLEKDIDKLEKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKDDKKDEDKNKDKDKDKEASQDKQSKDQTK
                     SSDKDNHKKPTSTDKDQKANDKHQS"
     gene            7631..7930
                     /locus_tag="RJE42_001383"
     CDS             7631..7930
                     /locus_tag="RJE42_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876808.1"
                     /translation="MHEQDFRILEGQDITLPELGKELENITGRTITDSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRTRLKEVPVHVELISYISKSK"
     gene            8010..8552
                     /locus_tag="RJE42_001384"
     CDS             8010..8552
                     /locus_tag="RJE42_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876809.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDSVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8647..9213)
                     /locus_tag="RJE42_001385"
     CDS             complement(8647..9213)
                     /locus_tag="RJE42_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDV5876810.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(9215..9673)
                     /locus_tag="RJE42_001386"
     CDS             complement(9215..9673)
                     /locus_tag="RJE42_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV5876811.1"
                     /translation="MTHIIIDGDACPVVDFIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVITQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLRVIHRIKELD"
     gene            complement(9676..10359)
                     /locus_tag="RJE42_001387"
     CDS             complement(9676..10359)
                     /locus_tag="RJE42_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876812.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKNSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10530..11405)
                     /locus_tag="RJE42_001388"
     CDS             complement(10530..11405)
                     /locus_tag="RJE42_001388"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV5876813.1"
                     /translation="MFIIELIKGIILGIVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSVKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIILVI
                     FIAILYFGFGIGKGI"
     gene            11624..13255
                     /locus_tag="RJE42_001389"
     CDS             11624..13255
                     /locus_tag="RJE42_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV5876814.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENINGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHINTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQNPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNKRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTVEEVLDEVGLLDKVQSFTQGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            13252..14925
                     /locus_tag="RJE42_001390"
     CDS             13252..14925
                     /locus_tag="RJE42_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDV5876815.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVSGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGNNRLTDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGKKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTILSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(15052..15495)
                     /gene="mgrA"
                     /locus_tag="RJE42_001391"
     CDS             complement(15052..15495)
                     /gene="mgrA"
                     /locus_tag="RJE42_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDV5876816.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            15724..>15830
                     /locus_tag="RJE42_001392"
     CDS             15724..>15830
                     /locus_tag="RJE42_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV5876817.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLK"
BASE COUNT         5544 a         2212 c         2727 g         5347 t
ORIGIN      
        1 gatttagcag tcttagattt aacgatgaag tattgcaaag atgaaaaagt acttatgcaa
       61 tcatttttaa aaactgcaat ggatgagcta gatttaagta ggacaaaatt attagtttct
      121 ataagaagac taattgaaaa agaaagactt agtaaagtta gatcatctaa agatgagcgt
      181 aaaatttata tttatttaaa tgatgatgat atatctaaat ttaatgcttt atttgaagat
      241 gtagaacaat ttttaaatat ttaattgaaa ttgagtgtcg aaagcataga aattgcttat
      301 cggcacattt ttaatttata catattttaa aactaagtga cagattgaag aaatcgtagt
      361 tcaataatgt taattgtgaa aatgtatata atcataaaaa aatcatgtat aatatatgtt
      421 gttaattaaa cagttcgaaa gcgagatgac attatgggac gtaaatggaa taacattaaa
      481 gaaaaaaagg cccaaaaaga taaaaataca agtagaatat atgcgaaatt tggtaaggag
      541 atttatgttg cagcaaaatc tggtgaacca aatccagaat ctaaccaagc tttaagattg
      601 gtacttgaac gcgctaagac atattcagta ccgaatcata ttattgaaaa agcaatagaa
      661 aaagctaagg gtgctggaga cgaaaacttt gatcacctaa gatatgaagg atttggccca
      721 agcggatcaa tgcttattgt tgatgcgtta acaaacaatg taaatcgtac tgcatctgat
      781 gtgcgtgctg cttttggtaa aaacggcggt aatatgggtg tatctgggtc agttgcttat
      841 atgtttgacc atgtggcaac gtttggtatt gaaggtaaat ctgttgacga tatacttgaa
      901 acattaatgg aacaagatgt agatgtaaat gatgtgtttg acgacaatgg attgacaata
      961 gtctatgctg aaccagatca atttgcggtt gttcaagatg cgctacgtgc agctggtgta
     1021 gaagaattta aagtagcaga atttgaaatg ttacctcaaa cagatattga gctttctgaa
     1081 gcggaccaag taacatttga aaaattaatc gatgcattag aagatttaga agatgtacaa
     1141 aacgtattcc ataatgtgga tttgaaataa tgaaatcagc agaacaatgg attgatgaat
     1201 tgcaacttga atcacatcct gaaggtggtt tctatagaga gacaatccga gaagtattga
     1261 aagatggacg cagagcgccg tttagtagta tttatttttt gcttacagat gacaatattt
     1321 cgcattttca tcgaattgat gctgatgaag tatggtacta tcatgctggt gattctctaa
     1381 caattcatat gataaatccg gatggggaat atacgactgc aacattgggt actgatatcc
     1441 aaaatggaga tgtattgcaa tatgtagtgc ctaaaggaac aatttttgct tcttcaatcg
     1501 aaatttcaaa tacttatagt ttagtaggtt gtatgtgtca accagcattt gagtttaagc
     1561 agtttgaatt gtttaagcaa tctgaattaa ttacacaata tccgcatctt aaatcagtaa
     1621 tcgaaaaata tgctttaaaa taaaaagaat caatgaagtg atttgaaggt tgttagtaaa
     1681 cctttgagtc acttcatttt tgtatgtatt ctgattcaat cagaatagaa ttgatgctac
     1741 agctttttct aatgaaatta acattaaatg gttttgaaaa tgataagact ataatattta
     1801 tatttttgaa aaatcactgt tatgaagaaa tatgtgcacg atctaattaa tattgtctat
     1861 tttaagtaaa ttgaggcgag ttgtagtaaa ataataataa atattttcat atgattaaca
     1921 aaaactataa aactgtatca tgacatgaca tcatccatgt agttttatat aataatgaca
     1981 aggagttggt gactgtgaaa gtaaagtata tagataaacg tcactggcgt cgcctaattg
     2041 atagggaata cacagaggta aaagttaata ataataggtt caagggtatt ataggcttag
     2101 tcacgatgaa aaaggttcgt gatcctttag aggtgacggt agttggacaa aatattattg
     2161 tcgcagatga caattataaa tggttgcaaa tactacctga aaagaaacgt tatagtatta
     2221 ctgtaatgtt tgataataaa ggcaatccat tagaatatta ctttgatata aatattaaaa
     2281 atataacgca aaaaggtaat gcgcgtacag tagatttatg tttagatgtt ttagcattac
     2341 ctagtggtga atatgagttg gtagatgaag atgatttaat gtttgcatta gaaagtgagc
     2401 aaattacaaa aaagcaattt catgaagcat atatgattgc gcatcaaatt atggcagagt
     2461 tagaaaatga ttttaaagga ttccaaaaga aaatcatgta ctgctttaat aaaattaatg
     2521 caaaggctca aaaaattcat caaaagccac aaaataaaac taattttgaa aaaagcaaac
     2581 aaataaaacc taagcaatat aatcaaacta aaaatcacca acaacaaaag aaaaactaag
     2641 taattcaagc tgcagccata ccaataaaat tggtgaaaat tattcgttag taatgaactc
     2701 ttgaatgctc gtagtcatga aagttcaata attgaataat ttatgggggg aaattatgaa
     2761 gattgaagac tatcgtttac taataacatt agacgaaacg aaaacgttac gtaaagcggc
     2821 tgaaatttta tatatatctc aacctgctgt tacacaaaga ctaaaagcta ttgaaaatgc
     2881 ttttggagta gatattttta tcagaacaaa aaaacaattg attacaacaa ctgaaggaac
     2941 aatgattatt gagcatgccc gtgacatgtt gaaaagagag cgattatttt ttgacaaaat
     3001 gcaggcacat attggtgaag tgaatggaac aatatcaata gggtgttctt cattgattgg
     3061 acaaacctta cttcctgaag ttttgagcct atataatgcc caatttccta atgttgaaat
     3121 tcaagtacaa gttggttcaa ctgaacaaat taaagcaaat catagagatt atcatgttat
     3181 gataactcgt ggaaataagg taatgaattt agctaataca catttattta atgatgatca
     3241 ttattttatt ttcccgaaaa atagacgaga tgatgttaca aagttaccat ttatagagtt
     3301 tcaagctgat ccgatttata taaatcaaat aaaacaatgg tataacgata atttagaaca
     3361 agattaccat gcaactatta cagtggatca agtagcaact tgcaaagaaa tgttgattag
     3421 tggtgtaggt gttacaatct tgccagaaat tatgatgaaa aatatcagca aagaacaatt
     3481 tgagtttgaa aaagtagaaa ttgataatga accgctgatt cgttcgacat ttatgagtta
     3541 tgatccgagt atgttgcaat taccacaagt tgattcgttt gtaaatctta tgacgagctt
     3601 tgttgaagaa ccaaaggcgt agtcttagac taatttaagg ttagtattta attttaagat
     3661 atacggttaa attgaacaca gttggatgct aatgcaccaa cacagaagct gacgaaaagt
     3721 cagcttacaa taatgtactg gggtgggacg acaaaataaa ttttgcaaaa atatcatttc
     3781 tgtcccactc ccataagagc ccctaattaa taaattaaaa ggggacaaag gaatacagtt
     3841 gattgctaac gcaccaactg cataagagcc cctaattaat aaattaaaag gggacaaagg
     3901 aatacagttg attgctaacg caccaactgc ataagagccc ctaattaata aattaaaagg
     3961 ggctctaaga agcggggtta tggatatgtt tgcagcgtta ttacaaataa agaattataa
     4021 actctttgtt gctaatatgt ttctactagg tatgggtatt gcggttacgg tcccatatct
     4081 tgttcttttt gcaactaaag atttgggtat gacaacaaat cagtatggat tacttctagc
     4141 atctgcagcg attagtcagt ttacagtaaa ttcaattatt gctagatttt cggatacgca
     4201 tcactttaat agaaaaatta ttattattct cgcattatta atgggtgcgc ttggtttttc
     4261 aatatacttt tttgtagata caatctggtt attcatatta ctatatgcga ttttccaagg
     4321 attatttgca ccagcaatgc cccaacttta cgcatctgct agagaatcta tcaatgtttc
     4381 aagctctaaa gatagagccc aatttgccaa tacagtatta cgttcaatgt tctcattggg
     4441 ctttttattt ggtccattta ttggtgcgca attaatcgga ttaaaaggct atgctggatt
     4501 gtttggtgga acaataagta tcattttatt tactttagta cttcaagtgt ttttctataa
     4561 ggatttaaac attaaacacc ctattagtac gcaacaacat gttgaaaaga ttgctcctaa
     4621 tatgtttaaa gacaaaacgc ttttattacc atttattgca tttattttat tacacattgg
     4681 acaatggatg tatacgatga atatgccttt atttgttact gattatttaa aagaaaatga
     4741 gcaacatgtc ggttatttag ctagtttatg tgctggttta gaagtgccat ttatgatcat
     4801 tcttggcgtt ttatcatcta gattacagac tcgaacattg ttgatttatg gagcgatttt
     4861 tggaggttta ttctacttca gcattggggt atttaaaaac ttctatatga tgttagcagg
     4921 acaggtgttt ttagctattt tcttagcggt tcttttagga attggtatta gttatttcca
     4981 agatatctta ccagatttcc caggatacgc ctcaacacta ttttctaatg caatggttat
     5041 tggacagtta ggcggtaact tattaggtgg tgctatgagt cactgggtag gtttggaaaa
     5101 tgtatttttt gtatcatcag catcaatcat gttaggtatg atacttattt tctttactaa
     5161 aaatcaaaaa attacaaaag aggatgtgat atcaacatga caattatttt atggctactt
     5221 atcatcgctg ccttcatgtt agcatttgtt gggttgatta agccgattat tccttctgtt
     5281 ttagtattat ggattggctt tctaatctat caatttggct ttcataatca gcatttatca
     5341 tgggtgtttt atgtatctat ggcattgcta acaatattaa ttttatgtgc ggacttttta
     5401 gctaataaat attttgtgaa tcgcttcggt ggttctaagt ttggagagta tgcagcttta
     5461 attggtgtcg ttattggatg ttttgtttta ccgccatttg gaattattat cattcctttt
     5521 attttggtat tcatagttga attaatacaa ggctattcat ttgaaagagc agttaaagta
     5581 agtataggtt caatcgtagc atttttaaca agtagtatag ctcaagcaat cattatgttt
     5641 ataatgattg tatggttctt tatagatgct ttattgatta attaataaaa agcttattgc
     5701 aaaatatgtt tttaggtaac tgtaatttag tgattttatc attaacagta ccaaattagc
     5761 atatgatgta ataagctttt tgtttataaa aatgtatgag aatgtttttt cgaaatattt
     5821 ctttcaatgc gtaatccaat aggcataact ataaatgaaa atataataaa tacccaattg
     5881 cttacctgaa tatatggtcc aaaggcaagt aatgattgtg gaataaagaa tacatagaaa
     5941 aatcctacaa ttagtagaat gattgctaaa taggtgaata tccatatcca atttgaattg
     6001 ttaaaacact gcagttgaat tgttttaaaa ctaaaccata taaataaaaa gacaataaag
     6061 aatccaacga ctactgaaaa cgggaatgaa acaaaatata aattacttcc acttttttcc
     6121 atgaaaaatc ctaaaaatcc tttgagaaaa ctaacaatac caattaatag aacgatgtgc
     6181 tgatatatat atttaaaaat atttttaaat gtttcattag gcatcgcttt tagttctttt
     6241 attgcatgtg cttttgggtc gtgattgaaa aaatctaagg ctaataaacc atgttgttct
     6301 gcgcttaata attgtttaag tatacggtta ataattaact ctgtatcatg aggattgacg
     6361 cgaaagtcag agcgcatata agtcatataa ttctcgaaga tttctctatc agtattgctt
     6421 aatcttaatg atttaacatt attttctttt gttaattgcg cagtactttt cattgttact
     6481 aaagcgctcc tttaaaaatg tttaattcca aactaaaatg gaaatgattt tatagtatta
     6541 ataaggtcaa tcatatcata ttaaacgcat aaatataacg attaatattg gagaagaaaa
     6601 tgaggacact taataaagat gaacataatt atatcaagca aatagctaat atacatgaga
     6661 cattattgtc gcaagcagaa tccaactata aatgtactaa actgagtatt gctcttaggt
     6721 acgagatgat atgttcaaga ttagaacata caaatgataa aatttatata tatgaaaatg
     6781 aaggtcaatt aatagcgttt atttggggac attttagtaa tgaaaaaagt atggttaaca
     6841 ttgaactgct atatgttgaa ccacaatttc gcaaactggg aatagctacg caactgaaga
     6901 ttgcgcttga aaaatgggca aaaactatga atgcaaagcg aataagcagt acaattcata
     6961 aaagtaattt accaatgata tctttgaata aagatttagg ttatcaagtg agtcatgtga
     7021 aaatgtataa agatattgat tagaattagg attatgttgc taattcatgt taaaattaaa
     7081 aaagatttaa tgacgttaag gagttttata tgaagaaatt aatcatcagt attatgtcag
     7141 ttatgatttt attagcaggt tgcggtaaaa gcgaagaaaa agcaactctt gaaaaagata
     7201 tcgataagtt agaaaaagaa aacaaagaat taaaagataa aaaagaaaag cttcaacaag
     7261 aaaaagaaaa attagcagac aaacaaaaag atcttgaaaa agaagtgaaa gatctaaagc
     7321 cgtctaaaga tgataaaaag gacgaagaca aaaataaaga caaagataaa gataaagagg
     7381 catcacaaga taagcaatca aaagatcaaa ctaagtcatc ggataaagat aatcacaaaa
     7441 agcctacatc aacagataaa gatcaaaaag ctaatgacaa acaccaatca taattgaatt
     7501 gcttacttgt tatagatgga aggtacagcg ttttaaacct tattttaagg gtatgtatta
     7561 attaaaatgt ggtcatgatt gaaacagaac gtaaaaatag acaacgtaat taataaagga
     7621 gagaaacggc atgcatgaac aagattttag aattttagag ggtcaagata ttactttgcc
     7681 agaattaggt aaagaattag aaaatattac gggacgtacg attactgatt ctactggcga
     7741 aattaagcgt gtaattgcac atttaccaaa ctttgagtcc gatacagata cttttgttgc
     7801 tacatatcgt ttaaaccatc aacaagattt tatagatgca acttttactg cgctgaaatc
     7861 agatagaaca cgtttaaaag aagtgccagt tcatgttgaa cttataagct atatttctaa
     7921 atcaaaataa actgctatct aaaacgcaaa gttgatcaaa atatcgattt tgtgtttttt
     7981 attgagaaat tatataggag tgtcaatcga tgatttattg tgaaacagag cgtttaatat
     8041 taagagactg gcatgaagat gatctgttac cttttcaaaa aatgaatgcg aattatgacg
     8101 tacgtaaata ttttccaagt ttattgagtt atcgtcgttc agaattagat atgagaacta
     8161 tggattcggt aattaaagat tatggcattg gattatttgc tgtagaagat aaagagtccc
     8221 atcaatggat aggatttata ggtttgaatt atattccaga aacaagcgat tatccattta
     8281 aagaattacc gctttatgaa ataggttggc gcttgttgcc agaattttgg ggaaaaggat
     8341 tagcaactga aggcgcaaag gcaacgttga agttagcaga acaacatcaa atatacgatg
     8401 tctatagttt tacagccgaa gcaaataaag cttcacaacg tgtaatggaa aaaattggca
     8461 tgacagtgta tgatcatttc gaattaccca atctaagtaa gtatcattta ttaaaaaggc
     8521 aagtgcgcta ttacattaat cttcgaaagt gaaaatttat acataagcgt aacaaacacc
     8581 cctaatattg tttagctgat gataacaata ttaggggtgt ttgtttattt tttaacctta
     8641 gaatgattaa tcgtatgaac gagtacctag aggtttgaaa ttcaatattg attctattaa
     8701 tgattcctta gtgtcgcata acggcgcaag tgcacgatac ttaggatcaa taaaaccttc
     8761 ctcaatcata tggtcaatca ttgtttgtag tggattgaaa aagccattaa tattataaat
     8821 ggcaataggc ttttcatgga tacctatttg agcccaacta tacatttcga aaaattcttc
     8881 tagtgaacct gcgccaccag gagccatgac aaatgcatct gcaagttctg ccattttatt
     8941 tttacgttca tgcatagaat caactaaaat taattcagtt aaacgttggc ttgtgatttc
     9001 atgttcatct aacattttag gcatgacgcc aatagctttg ccaccatgat ctaatacacc
     9061 atcttgaatg gcacccataa taccaattga ccctgcacca aatactaatt cataaccttg
     9121 ttcagcaaaa tatttaccta aatcgtatgc tttttgtaca tatgaagggt catgaccttt
     9181 gcttgcacca caataaactg cgattcgctt catattaatc cagctcctta attcgatgaa
     9241 tgactcttaa tagtgattgt tcaaacactt tttgatcttg ctttgtaaaa ggtgggggac
     9301 ctttgtggcg accaccttgt tttctaattt gtgcattcat atatcgttta tctaatagtt
     9361 gttgaatatt tttggaattg tatatcttcc cattatgatg catgacaatt aagactttgt
     9421 cgactaataa acttgcgagt ccataatctt gagtgattac gatatcatct ttcgttgata
     9481 attgaacaat tttgtaatca actgcatctg gtccatcatc aacatataat gttgatacat
     9541 gtgggggata taattggttc gaaaaatggc tgaagctccg aataattgtc acaaaaatgc
     9601 ctgtctcagt tgttaaatct ataataaaat caacaacagg acaagcatct ccatcaataa
     9661 taatatgtgt cacaattatg cctctgtatt gttttcttta ttttgttgag aggcgctttt
     9721 ggcaacataa tctttatatt ttttaaatga cttgatgcgt gctttatcag cttcttgttg
     9781 gcgtttttgt tcttctttgt gtcgtttttc aatatttttt tgtaactttt tattcatttt
     9841 agcgatttct ttgcgatttt tttcagctag tttatcgcct tttttctcag ttttctcatc
     9901 taatttatta ggtgttaagc ctgctttttc ttcgtatttt tgtgagtttt tcatatcttt
     9961 aatacgttgt atttcattct tttcgcgggc tttttgctct tctttatgac gcttttcgat
    10021 atttttttga agtattttat tcattttatc agcgtcttta cgattttgtt tagctaattt
    10081 ttcgcctttt ttctcaatat aggcaggatc atgttctcta gcaaactttt taagttcacg
    10141 cttattttca aaatcttgct ttttatcgcc gacatattct ttaacatcac tcgctgtgtt
    10201 actgattgct gcagatgttt ttgaagcaac tttacttgta gcatctgtaa ctttttgtac
    10261 gtccggatgt tgtttgatac gtttacgttc aacaattaac ggtaccaata caattggtaa
    10321 tacattaatc ataaatttga tgactttttt cttatccata gatcttgcct ccataattac
    10381 tttattaatt ttacataccc tatgatacat caatataaac gatgagagta gtgaatcact
    10441 tataagtatt tcagatgttt ttaaaagaag acaataaaaa ctgccaatca agtggttcct
    10501 taattgacag tctatatttt aaaaggaaat taaatacctt taccaatccc aaatccgaag
    10561 taaagtatag caataaagat tactaatata attctgtaaa tggcaaatgg aattaatttg
    10621 attttgttaa ttagatgcaa gaatgttttg attgcaatta gtccaacagt aaatgcagct
    10681 aaaaagccta aaatataaaa aggtatatca gcaatctgaa tatcttgata atgttttaat
    10741 aaagataaac cacttgctgc caacataatt ggaacagcca taataaatgt gaagtccgat
    10801 gctgctttat gatttaattt cattaataca ccagttgaaa ttgttgagcc tgaacggctg
    10861 aaaccaggcc acatagctac tgcttgagag ataccaatta caaacgcttg gaaataattg
    10921 atttgatcca ctgtttgtgg gtttttaact ttaactgaat atttatcagc gataatcata
    10981 tagatagcac ctacgaataa gccaatcata acagttggca cactaaataa atgttcttcg
    11041 atgaaatcat caaatagtaa gcctaaaata cctgctggca ccatacccac taatacatgt
    11101 aataaattta aacgtcttgg ctttgaacgt ctttgttgat tgttatctcc ttcaacatgt
    11161 ttgtgtttac caatatgtaa aatctctaag aagcgttcgc ggaacaccca tgctgctgca
    11221 aagacggatc ctaattggat gacgatttta aatgtaaatg ctgattgaga acctaaaaat
    11281 tcagatgatt ttaaccacat atcatcaact agaatcatat gtccagtaga ggaaacaggg
    11341 gcaaattccg ttaatccttc gacgatccct aagataatac cttttattaa ttcaatgata
    11401 aacataatgt acccactttc attactcaat ttaatttatt taaatatcaa aattaccata
    11461 tcatgatagc atattcattt aaagacatgc tagttatagt tataatacta gactaaagat
    11521 gtatatattc attttctttt acatgtaaaa ctacaatatt ttattgagct atttaatttg
    11581 attttaagga aaacctttta taataagttt aggtgatata attgtgaaaa aattaacaac
    11641 aatactgttt caatataaaa tttttccggt actcatgttc ttggtcagta ctggtctcgg
    11701 catactcgtt ataacgcaaa atattttaat agcagatttt ttagctaaaa ttataagaca
    11761 tcaatttcaa ggtttatgga ttgtattatt tattttatta ggtgttttac ttttaagagc
    11821 aactgtgcaa tttctaaatc aatggttagg tgatacatta gcatttaaag ttaagcatat
    11881 gcttagacag cgggttattt ataaaaataa tggtcatcca atcggtgaac aaatgactat
    11941 actcacagaa aacattaatg gtctagcacc tttttataag agttatttgc ctcaagtgtt
    12001 caaatcaatg atggttccgc tcatcataat cattgcaatg tttttcatcc atatcaatac
    12061 cgcattaatt atgttaataa ctgcaccatt tattcctttg ttttatatta ttttcggttt
    12121 gaaaacgcga gatgagtcaa aagatcaaat gacttattta aatcaattta gtcaacggtt
    12181 tttaaatatt gctaaaggtt tagtgacgtt aaagctattt aatcgtacag agcaaacaga
    12241 gaagcatatt tacgacgata gtactcagtt tagaacttta acaatgcgca ttttacgcag
    12301 tgctttttta tcgggattaa tgctcgaatt tataagtatg ttaggtattg gattggttgc
    12361 attggaagca acgctaagct tagtagtatt tcataatatt gattttaaaa ctgcggcaat
    12421 tgcgattatt ttagcgcctg aattttataa tgcaattaag gatttaggac aagcgttcca
    12481 tactggaaaa caaagtgaag gtgccagtga cgttgtgttt gagtttttag aacaaccgaa
    12541 caataataat gaatttctat taaagtatga ggaaaatcaa aatccattta ttcagttaac
    12601 agacatatca tttcgatatg atgattctga tagattggta ttaaatgatt taaatttgga
    12661 aatatttaaa ggtgatcaaa ttgcacttgt aggtccaagc ggggcaggta aatcaacttt
    12721 gacacatctt attgcaggtg tttatcagcc aacaataggt actataagta caaacaagcg
    12781 tgatttaaat ataggaatac ttagtcaaca gccatatatt ttcagtgctt ctataaaaga
    12841 gaatattacg atgtttaaag atatagaaaa taatactgtt gaagaagtgc tagacgaagt
    12901 aggtttatta gacaaagttc aatctttcac acaaggcatt aatacaataa taggtgaagg
    12961 aggcgaaatg ttatctggtg gacagatgag acgcatagaa ctttgccgtc ttttagttat
    13021 gaagccagat ctcgttatat ttgatgagcc tgcaactggt ttagatattc aaacagaaca
    13081 catgattcag aacgttctgt ttcaacattt taaagataca acgatgattg tcattgcaca
    13141 tagagataat acaattcgcc atttacaacg acgtttgtat atagaaaatg gaagactgat
    13201 tgctgatgat cgaaatattt cagtaaatat aacagaaaat ggtgatgact tatgaaaaca
    13261 cgactaaaat ttcaagtaga taaggattta ttgttagcta tagttgttgg tgttagtgga
    13321 agtttagttg cgctcgccat gtttttctta agtggttata tggtgacaca aagtgcactt
    13381 ggtgcgccac tatacgctct gatgatttta gtcgttacag taaaattgtt tgggttttta
    13441 agagctatta ctcgatacgt agagcgcctt atttctcata aagctacatt tacaatgcta
    13501 cgtgatattc gggtacagtt tttcggtaaa ttagtaaatg tcattcctaa tgtttaccgt
    13561 aaactgagtt ctagtgattt aatttcacgt atgattagtc gtgttgaggc attacaaaat
    13621 atatatttac gtgtttatta tccaccagtc gtcatcggtt tgacagcgct agttacagta
    13681 atagtattgg cgttcatttc aatcggccat gcgttattga ttatggttag catgttgttt
    13741 actttactca ttgttccttg gttaagctca aaaaaagcac gtactttaaa gaaacatgca
    13801 gctaatgaac aggctcgatt tttaaatcat ttttatgatt ataaagctgg tatggatgaa
    13861 ctacgtcgat ttaatcaaat taatcattat cgagataatt tgatggctaa attaaatcat
    13921 tttgataaat tacaacttaa agagcaacgc tttttaacga tttatgattt tatattaaat
    13981 attattgcta tgctttcgat ttttggtagt ttagttctag gattaattca aattaatgca
    14041 ggccaactaa atattattta tatgacgagt atcgttttaa tggtcttaac tttatttgaa
    14101 caagctgtac caatgacaaa tgtcgcgtat tataaagcgg atactgacca agcattgcac
    14161 gatattaatg aagtgatatc tgtaccttct actaatggaa ataaccgcct tactgataag
    14221 tatgacgcaa cgaacattta tgaagttaag gacgctagtt ttaagtattg gaaccagcaa
    14281 acgtatgtgt tatcggatat taattttaat gttaatagag gcaaaaagat tgcgattgtg
    14341 ggtccttctg gttcaggaaa aagtacatta ctacaaatta tggcagggtt atatcaatta
    14401 gatagtggct ctattcgttt cgaaaatatg gacatgtttg aaatagatga caaagataag
    14461 tttgaatcgt taaatgtctt gctacaatct caacaattat ttgatggtac aatacgtcaa
    14521 aatttattta ccgatgaaaa agatgaagcg gtgcaagcaa tatttaagca attagattta
    14581 gaacatttgg cactagaacg tcaaattgac ttagatggtc atacattatc tggcggagaa
    14641 attcagcgtt tagcgattgc gaggatgtta ttaaaagata ctgcatcaac atggatttta
    14701 gatgaaccaa caacggcatt agataaacaa aatagtttaa aagttatgga tttaattgaa
    14761 gcacatgcag aaacattaat tgttgctaca cacgatttaa ctatattgtc acgttttgag
    14821 accatcattg tgatgataaa tggtaaaata gttgaaaagg gaaactatca acaattactc
    14881 gctaatcaag gtgctttatg gaatatgatt caatataatg cataaaaaaa ctgcttgata
    14941 ggctaagaaa cctttcaagc agttttttaa ctaacaagct tacgcgtgct aattgttttt
    15001 ctattcttaa taaattctaa acattacttt aattgtcatg acaaaagtta attatttttc
    15061 ctttgtttca tcaaatgcat gaatgacttt acctaataag cgattaagtt ctttaacttc
    15121 atcttgagat aaagaagaag ctgaagcgac tttgtcagat gcattactta attctggtct
    15181 aatagtttca cttttgtcag tcaagtgaat aaatacttca cgttgatcga cttcggaacg
    15241 ttcacgctta attaagtcta cttgttccat tcgttttaat aatggtgata ctgtaccagt
    15301 atcgagtgct aattcagtta cgactttctt gacgtttaca ggagattcat cccataaaat
    15361 agttaagaca agaaattgtg ggtatgttag attgtacttc ttaaaaactt tgttagagta
    15421 gtagcgatta acttgtcttt gagcattgta caaactaaag catagctgtt cttttaaatt
    15481 atgttgatca gacattaaag ttctcctcca gacatactat ccgttttttt ctcttttcgg
    15541 attggtaatc attaaaaagt tgattgttta ttaattcaca actttctttg attcaatgcc
    15601 atgctaaaat taaagtatgt ttaaagttta gaagatattt ttgattaaat caagcaaaaa
    15661 gataatttaa tatatatatg ttatcatttt taaaaataac tgtaatagaa aagagaatat
    15721 aaaatgaaaa ataataaaga tgaaaaaata agaatatcca taattaacgg atttttgggt
    15781 agtggtaaaa ccacgttact gacacattat attagtgaat tattaaaaaa
//
LOCUS       DAPIDM010000050        15628 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000050 DAPIDM010000000
VERSION     DAPIDM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.050"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            126..1202
                     /locus_tag="RJE42_001393"
     CDS             126..1202
                     /locus_tag="RJE42_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV5876818.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMTAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLYKSAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            1227..2267
                     /locus_tag="RJE42_001394"
     CDS             1227..2267
                     /locus_tag="RJE42_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV5876819.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            2322..3290
                     /locus_tag="RJE42_001395"
     CDS             2322..3290
                     /locus_tag="RJE42_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV5876820.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPTQAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(3648..4142)
                     /locus_tag="RJE42_001396"
     CDS             complement(3648..4142)
                     /locus_tag="RJE42_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDV5876821.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            4375..5754
                     /gene="uhpT"
                     /locus_tag="RJE42_001397"
     CDS             4375..5754
                     /gene="uhpT"
                     /locus_tag="RJE42_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV5876822.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(6114..6872)
                     /locus_tag="RJE42_001398"
     CDS             complement(6114..6872)
                     /locus_tag="RJE42_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5876823.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVVLGRLVRTLLEQQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTISDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(6865..8421)
                     /locus_tag="RJE42_001399"
     CDS             complement(6865..8421)
                     /locus_tag="RJE42_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDV5876824.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSIHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEALHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(8418..9386)
                     /locus_tag="RJE42_001400"
     CDS             complement(8418..9386)
                     /locus_tag="RJE42_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDV5876825.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNDFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            9974..12223
                     /gene="pflB"
                     /locus_tag="RJE42_001401"
     CDS             9974..12223
                     /gene="pflB"
                     /locus_tag="RJE42_001401"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV5876826.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            12246..13001
                     /gene="pflA"
                     /locus_tag="RJE42_001402"
     CDS             12246..13001
                     /gene="pflA"
                     /locus_tag="RJE42_001402"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDV5876827.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            13323..15086
                     /locus_tag="RJE42_001403"
     CDS             13323..15086
                     /locus_tag="RJE42_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDV5876828.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINDWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVVFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSSLFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFVGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNRHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     TLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(15250..15594)
                     /locus_tag="RJE42_001404"
     CDS             complement(15250..15594)
                     /locus_tag="RJE42_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDV5876829.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
BASE COUNT         5209 a         2400 c         2981 g         5038 t
ORIGIN      
        1 tagaaaaagc atttctttat gttggggccc cgccaatata acattgtaga gcctagaacg
       61 ttgattgatg tttaaggctc gacatacgca ctaatgacga taaagattta aaggaggacg
      121 ttatgatgac gattaaagtt ggaatcattg ggtgtggtgg tattgcgaat ggcaagcaca
      181 tgccaagttt acaaaaagtt gaaaatgttg aaatgaccgc attttgtgac gtagacattt
      241 cgaaagcagc gagtgcggca gaagcatacg gtactgacaa tgcaaaggtt tatgatgatt
      301 acaaagcatt gttaaaagat gacacgattg atgttatcca tgtttgtacg ccaaatgact
      361 cgcattgtga aattactgta gcagggttgc atgccggtaa acatgtgatg tgtgaaaaac
      421 caatggctaa aacgacagca gaagctcaaa aaatgataga tacagctaaa tcaacaggta
      481 aaaaattaac aataggttat caaaatcgct tcagaccaga tagtcaattt ttatataaat
      541 cagcgcaacg tggcgactta ggtgacattt atttcggaaa ggcacatgct attcgtcgtc
      601 gagcagtacc aacatggggt gtctttctag acgaagaagc tcaaggtgga ggaccattaa
      661 tcgatatcgg tacacacgct ttagatttaa cgttatggat gatggataat tatgaaccag
      721 aatcagtgat gggttcaaca ttccataaat taaataagca gcatgatgct gcaaacgctt
      781 ggggttcatg gaatccagat gaatttacag ttgaagattc tgcgtttggc tttattaaaa
      841 tgaagaatgg agcgacgatc attttagaat ccgcttgggc gattaattct ttagaagtgg
      901 atgaggcaaa atgttcatta tcaggaacga aagcaggtgc tgatatgaaa gatggtctac
      961 gtattcatgg tgaagatatg ggcacacttt ataccaaaca cgttgaattg gaaaacaaag
     1021 gcgtcgactt ttatgaaggt aatgaagtgg atgaagctga agaagaagca aaagcttgga
     1081 ttgatgcagt tgtaaatgat actgaaccag ttgtgaaacc ggaacaagca atggtagtta
     1141 caaaaattct tgaagcgatt tatcagtctg caaaatcagg caaagcaatt tactttgaat
     1201 aacatcatac ggtaaggagg cacatcatga caaaattaaa agttggtgtg ataggtgttg
     1261 gtggtattgc acaagaccgt catattccag cattgctgaa actcaaagac acagtctcgt
     1321 tagttgcagt acaagatatt aatacagtgc agatgattga tgttgcgaag cgctttaata
     1381 tacctcatgc agttgagaca cctagtgagc tgtttaaact tgttgatgcg gtggtcattt
     1441 gtacacctaa taaattccat gctgatcttt ctatagaagc attgaaccat ggtgtccatg
     1501 tattgtgtga aaagccaatg gcaatgacga cggaagagtg tgatcgcatg attgaagcgg
     1561 ctaataaaaa tcacaaatta ttaactgtcg catatcatta tcgccatacg gatgtcgcaa
     1621 ttactgctaa aaaagcaatt gaatcaggtg tggttggtaa acctttagta gcacgtgtac
     1681 aagcgatgcg taggcgtaaa gtgcctgggt ggggtgtttt taccaataaa gcgttgcaag
     1741 gtggcggtag tttaattgat tatggttgcc acttgttaga cttatctttg tggctactag
     1801 gtaaagatat ggtgccgcat gaagtgctag ggaaaacata taatcaattg agcaaacaac
     1861 cgaatcaaat taatgattgg ggaacatttg atcacactaa atttgatgtc gatgatcatg
     1921 ttactagtta tatgacattt gccaatcgag caagcatgca gtttgaatgt tcgtggtctg
     1981 caaatataaa ggaagataag gtacacgtta gtttatcagg cgaagatggc ggtatcaatt
     2041 tatttccatt tgaaatatat gagccccgct ttggaactat ttttgaaagc aaagctaatg
     2101 ttgagcataa cgaagacatt gctggtgaga gacaggcgcg taactttgtc aatgcatgtt
     2161 tagggataga agagattgtg gtgaaaccgg aagaagcacg caatgtaaat gcccttatag
     2221 aagcgattta tcgtagcgat cttgataaca agagcataca actttaatga ttattatata
     2281 taatacaaaa ttctcaatat aaaaagaagg agtgctttgc aatgaaaata ggtgtatttt
     2341 cagtattatt ttacgataaa aatttcgaag atatgttaga ttatgtcgca gaatctggat
     2401 tggatatgat tgaagttgga acaggtggta acccaggaga taaattttgt aagttagatg
     2461 agttgttaga aaatgaagac aagcgacaag catttatgaa gtcaatcaca gacagaggct
     2521 tacaaataag tggtttcagt tgtcataaca atccaatttc tccagatccg acacaagcga
     2581 aagaagccga tgaaacgtta cgtaaaacca tccgtttagc aaatctatta gacgtgccag
     2641 ttgttaatac attttctggc attgcaggat cagatgatac cgctaaaaag cctaattggc
     2701 ctgttacacc atggccaaca gcctactctg aaatttatga ttatcagtgg aatgaaaagt
     2761 tgataccata ttggcaagat ttagctgagt ttgcaaaaga gcaagatgta aaaattgcca
     2821 tagagttgca tgcaggattt ttagttcata caccatatac gatgttgaag ttacgtgagg
     2881 ctacaaatga gtatatcggt gctaacttag atcctagtca tttatggtgg caaggtatcg
     2941 acccaattgc tgcgattcgc atattaggcc aagcaaatgc aattcatcac ttccatgcta
     3001 aagatacgta cattaatcaa gaaaatgtaa atatgtatgg tctaactgat atgcaaccat
     3061 atggtaacgt tgcaacaaga gcatggacat tccgtacagt tggttatgga catagtccat
     3121 atgtatgggc agatatcata agtcaactta ttattaatgg atatgattat gtattaagta
     3181 ttgaacatga agatcctatt atgtcagtag aagaaggttt ccaaaaagct tgtcaaactt
     3241 tgaaatctgt taatatttac gacaagccag cagacatgtg gtgggcataa tacgaacgcg
     3301 aggttagtct gaagtttgtc tgaagtaaga ctgatggcag tgtcgaataa atgcatatat
     3361 cgccaagcca ttgccaaaaa tttcacacct taaattaagt cattgtttgt aaagaaggtg
     3421 tactttatat aagtatatag cgatggtcat acccattcac agtaacaatc ctcaccattg
     3481 aaaagagtat ataacctttt caatagtgag gtatatgata ataaaaaagc ctgttgtcac
     3541 aatggtcata gacacgacat actttaaagg tttctgaata taatatttca aaacgcactt
     3601 taaagatgga cgtcgatgtc gactaatgtg atgacaggct ttcattttta aatattcatt
     3661 aatttctctt cttgtttaat acgtacatat aagaaatacg catacggtac taataaaata
     3721 gttgtatatg ttgcgtgtgt taataataat acaccgatta attcaggaat gatgtttaag
     3781 aagtaatttg ggtgttttgt aattttatat aatccagatt taataatagg atggttaggt
     3841 aaaatgaata attttaatgt ccaaatacca cctaaagttt taataaccat aaataacatg
     3901 atataagcaa agattaatat aactaagcca ataccatttg caaagctaaa tgtatcttta
     3961 ttaataaatg cctctacacc agccaataca taaattaaaa cgtgtgttat tgctaaaaac
     4021 ttcgaatttt taacgccata ttcaactgca ccgtctgctt ttaattgttt tgagtgatta
     4081 atagatatct ttaagctgac aagtctgata cagaaaaaga taagtaatat agatagaatc
     4141 atgatgtcct ccgtcattat gtcatatgta taagcgttga ttttgacaac ataaagtatt
     4201 ttatagataa aacttgtcaa atactattaa ctatttatta attttagtac ataaatatgt
     4261 ttctaagtat gtgtttatgt tcagtatttt ggataattta ataattttaa ggatattaag
     4321 cgcttacacc gacgtgatat atttggctta acgaaaatga ttgaggtgac agagatgaac
     4381 ttttttgata tccataagat tccgaacaaa ggcattccat tatcggtaca acgtaaatta
     4441 tggcttagaa acttcatgca agctttcttc gtagtgttct ttgtttatat ggctatgtat
     4501 ttaattcgaa acaactttaa ggcggcacaa ccgtttttaa aagaggaaat tggattatct
     4561 acattagaac ttggttatat cggattagca tttagtatca cgtacggttt agggaaaaca
     4621 ttacttggat attttgtcga tggacgtaac acaaaacgca ttatctcatt cttacttatc
     4681 ttatctgcga ttacagtttt aattatggga tttgtgttaa gttactttgg ttctgtaatg
     4741 ggattattaa ttgtactttg gggacttaac ggggtgttcc aatcagttgg tggacctgca
     4801 agttattcaa cgatttcaag atgggcacca agaacgaaac gtggtcgata cttaggattc
     4861 tggaatacat cacataatat cggtggtgcc attgcaggtg gtgtcgcact ttggggtgct
     4921 aatgtattct tccatggaaa tgttatagga atgttcattt tcccgtcggt gattgcatta
     4981 cttattggta tcgcaacatt atttatcgga aaagatgatc cagaagaatt aggatggaat
     5041 cgtgctgaag aaatttggga agagccggtt gataaagaaa atattgattc tcaaggtatg
     5101 acaaaatggg agatctttaa aaaatatatc ctgggaaatc ctgttatatg gattctatgt
     5161 gtttcaaacg tctttgtata cattgtacga atcggtattg ataactgggc accgttatat
     5221 gtgtcagagc atttacactt tagtaaaggc gatgcagtta atacgatatt ctactttgaa
     5281 attggtgctt tagttgcaag tttattatgg ggctacgtat cagacttatt aaaaggtcgt
     5341 cgtgcaattg tagctattgg ctgtatgttt atgattacat ttgttgtctt attctacaca
     5401 aatgctacaa gtgtaatgat ggttaacatt tcattgtttg cattaggtgc gttaatcttt
     5461 ggtccgcaat tattaattgg tgtatcattg actggttttg ttcctaaaaa tgccatcagt
     5521 gtagcaaacg gaatgacagg ttcattcgcg tatctattcg gtgactcaat ggcaaaagtt
     5581 ggtttggcgg ctattgctga tccaacacgt aacggtttaa acatctttgg atatacatta
     5641 agtggatgga cagatgtttt catcgtcttc tatgttgcat tattcctagg tatgattcta
     5701 ttaggaatcg ttgctttcta tgaagaaaag aaaattagaa gtttaaaaat ctaatatcaa
     5761 tcggattaaa agtatcgcca atttgttgca gtatagttgg caatcctgcc ccgacggcat
     5821 gtgcgtgaag agatgaaaga tactgcttct acccttgcaa atatatcatc tctatgtctc
     5881 ggggcagatc ataattccct gttatgaagt atccttattt gcccgactta gggtgactca
     5941 atgaatttac tccttacaat aaagacatat agcggtgtca atattgtagg gagtattgtt
     6001 tttatattta aactctctaa aaagcggact gaaagaaaag tgaaaacttc tctagcagtc
     6061 cgctttttca tagaacaaaa tgtaggcgcc ataatcatta gttatttgct aatctatttt
     6121 gcttgcttac aataatcact tggcgacatt tgtaaatatt ttttaaaatg atagctaaac
     6181 attttatact ctgaaaagcc tactttgtct gcaatttcat agtgtttata atgtcgatct
     6241 aacaattgca gagattgtaa aatacgatag cgatttaaat aatcgacaat tgtaatacca
     6301 acatgatctt taaatgttcg catcgcatac gattcactaa catcgatatg ttgaattaaa
     6361 tctgaaatag tcactttcgt ttgataagat tgcttaattt gatcaacaat ctggtttaca
     6421 taataatcat cgtattctac ttttaatagt ggttggaagg catcatgaca aggtgctaag
     6481 ctacggccgt tctgtgattg ttgctctaat aaggtacgga caagtcttcc taaaacaact
     6541 tctaattgtg catggtccac tggttttaat aaataatcaa gaacatgatg ttgaatgccg
     6601 gctttcatat attcaaagtc atcgtaactc gataatatga tgacattaca atctagatgc
     6661 gcaatatcat tgagtaaatc gacgccattt ttacgtggca tacgaatatc agtaattact
     6721 aattctggct gatgttgttg aattagtgat aatgcttcaa caccatcttt agcagtgtat
     6781 attgtattga aatgatagtc tccccaagga atgatttgct ttaaaccttc tcgaataatt
     6841 cgctcatcat cacaaataac taccttaaac atctacattc ccccttgaaa gtggtatttt
     6901 ataacaaatt aacgtacctt gattacgctt tgaaaaaata tggagtcgtg catgtgaacc
     6961 atattgaatc attgctttat tgtgtaaatg atttaatccc aaatgcttag tatcaaatac
     7021 atcattatta agagattggc gtacatattg caggcgagat gacgacatcc caataccatt
     7081 gtcgcaaact aaaacatgta aattctgacg tgccaatgtc aggcgtatag taatgtccaa
     7141 tgactcagta tctctaccat gtttaatagc attttctatg agtggctgaa gcatcatttt
     7201 accaattgtc tggtgaagcg cttcttcaga actttcgata tggagcttaa tcatgtcatc
     7261 aaaacggatg ttttgtatcg caacatactg ttcaatgtag ttcaactctt cgtttaattc
     7321 cactgtatgt gagtttgtac gtaatgagta acgtaacatt tgcgataatt gttgaaccac
     7381 agtttgtgct aatttcggag ataacgtaat taaatattgt attgtttgca tcgtattgaa
     7441 taggaaatga ggttggaatt ggcgttctat ttcctttaac tgaatatcac gcaagcgacg
     7501 ttctgtatgc tcaatagaat ggatcagttg ctcatttgat tcaaataaat cgtaaatata
     7561 attattaatt tcttctagtt cactgttgtt ttttaaaggc gtatatgtac ctagatgacg
     7621 atttttggca tagtaaattt tttgaataat cgtttcgata tcttttgttt gtcgtttagc
     7681 catattatct gcactaatga aaccaaatat tactagtaaa acaagaacta cagccataac
     7741 aattaacaac gtgataccat cttcaatgtt ttcatggata tctttataaa taatgagacg
     7801 atggtcagca tggtttaatt ttacagattc attcataaat ccgaattgtt gcggtctata
     7861 cttttcacct atagtaaaac ggtcatcgtt ggcgtataaa atattgtcat attgatcaac
     7921 gataagtgcg aattgtcggc tatctttctt aatttcactt aaacgtgggg tgttagccat
     7981 ataaatttta agcatatatg tactattttt gaatttaagc tgatgcgttg aaaataaata
     8041 catattttta gtgtttaaat gttcataatt attggttata aactgatttg gtccagataa
     8101 ttcataataa agtgttgcgg gctgttggtg tattaatttt aataattcac gttttgtagc
     8161 ggtaacatca tgatgatttg ttaaatcaag ctcttgaaac gaattgttat gctgtgtaat
     8221 aaatgtctga atctgctttt cagtatgatg tatagatgac tgactttcat caacatgttg
     8281 atgaatcgta cgatgctcaa tccaaatata gatggcatag aagcttacta gtccaataat
     8341 aatgactaaa aatactggaa aaatagtaga cgcaaataac gatcgtctta attgatgtct
     8401 ataaggtttg tatgccgtca ttgaatcatc tccaaaaatt tatgatgtgg aatgtccggt
     8461 aatttagatt tcggtattaa aggtatgttc ttaagatttt cgatagactg atcgctttgt
     8521 tcactaacat cctttcgaat tgacttggca tcgaactctg caactaatcg ttgttgtact
     8581 gagcggcttg ttaaatattg cactaacttt ttacgcttag gatgagggtg tgcattttta
     8641 actaaagcaa taccatcaac atttaacatt gttccttcaa ttggataaac gattgagaca
     8701 ggataacctt tgtttttcca tgtgcgtgca tcttgttcgt agcttagacc tgcgtaatat
     8761 ttaccttttg caacatcttc aatgacttta gacgtctttg acagttgcat cgcgtggtct
     8821 tggaattgat gcacatcact tactcgatga tgcatgctat aaatagcgcg catatgttga
     8881 tagcctgtcg ttgttgtatt tggatttgag tacgcaattt tacctttaag tataggttgt
     8941 aataaatctt gataacctcg aatcttaata tctccttgta aatctgaatt aactactata
     9001 actgttggca ttaatagaaa actagtaaca tatttattgt tcgagcgata atcctctaat
     9061 tgctgtgtta cagatgtatc ttgataggga acaaaatctt ctggatgatc aattgtttct
     9121 gacaacacac cacccataaa gacatcacca cgctccgaaa aatcttcgtt atgcaagttt
     9181 gaaagcagta cttgagtaga tccgtgttta atttcaattt tgacatgctc ttgtttttca
     9241 aaatcattta aaattggacg aatcaagttt gattgatacg gagaataaac tgttaataca
     9301 tttttatctg attcagagtg acgcgtatta gcgcatgctg ataaaaaaat gagaaataat
     9361 agtaagatat aaatttttga tttcatgata tcccatcaat tctatgtata ttttaataca
     9421 ataattttag caataaatga cgcataagta atgttaaata tttagaaatg tttatagatg
     9481 acttgttaag acgttgcaaa tgttgtgata gcacataatt tttgtttgtc aagacgactt
     9541 accgaagctg taaaatcaaa ctgttatatt ttatttgtag ctgttatata aaaattggca
     9601 agatattgaa tggttcaaaa gtaaattttt acgtcaataa aagtatttaa tcctgtctct
     9661 tcatatataa aagtaaatct ttctaagtat tgatttaacg cttattaaca atcatttttt
     9721 ataaacaaat atatccacct aaattaactt ttaaagcaat gaaaatagtg aacattataa
     9781 ctgttgtgta acagaatgca attagcatat tactgttaca caaattagta cagtttctat
     9841 gttttgacat acatttgatg aaaattgtac ataatttatg tgaaaaaaat cacaacaaac
     9901 atgctacaat gactatgaaa acgttaacat agcatttcaa attcacaaca ttatacagat
     9961 ggaggcgttt agtatgttag aaacaaataa aaatcatgca acagcttggc aaggatttaa
    10021 aaatggaaga tggaacagac acgtagatgt aagagagttt atccaattaa actacactct
    10081 ttatgaaggt aatgattcat ttttagcagg accaacagaa gcaacttcta aactttggga
    10141 acaagtaatg cagttatcga aagaagaacg tgaacgtggc ggcatgtggg atatggacac
    10201 gaaagtagct tcaacaatca catctcatga tgctggttat ttagacaaag atttagaaac
    10261 aattgtaggt gtacaaactg aaaaaccatt caaacgttca atgcaaccat tcggtggtat
    10321 tcgtatggcg aaagcagctt gtgaagctta cggttacgaa ttagacgaag aaactgaaaa
    10381 aatctttaca gattatcgta aaacacataa ccaaggtgta ttcgatgcat attctagaga
    10441 aatgttgaac tgccgtaaag caggtgtaat cactggttta cctgatgcat acggacgtgg
    10501 acgtattatc ggtgactatc gtcgtgttgc tttatatggt gtagatttct taatggaaga
    10561 aaaaatgcac gacttcaaca cgatgtctac agaaatgtca gaagatgtaa ttcgtttacg
    10621 tgaagaatta tcagaacaat atcgtgcatt aaaagaatta aaagaacttg gacaaaaata
    10681 tggtttcgat ttaagccgtc cagcagaaaa cttcaaagaa gcagttcaat ggttatactt
    10741 agcatatctt gctgcaatta aagaacaaaa cggtgcagca atgagtttag gtcgtacatc
    10801 aacattctta gatatctatg ctgaacgtga ccttaaagca ggtgttatta ctgaaagcga
    10861 agttcaagaa attattgacc acttcatcat gaaattacgt attgttaaat ttgctcgtac
    10921 acctgattac aatgaattat tctctggaga cccaacttgg gtaactgaat ctatcggtgg
    10981 tgtaggtatt gacggacgtc cacttgttac gaaaaactca ttccgtttct tacactcatt
    11041 agataactta ggtccagcac cagaaccaaa cttaacagta ttatggtcag tacgtttacc
    11101 tgacaacttc aaaacatact gtgcaaaaat gagtattaaa acaagttcta tccaatatga
    11161 aaatgatgac attatgcgtg aaagttatgg cgatgactat ggtatcgcat gttgtgtatc
    11221 agcgatgaca attggtaaac aaatgcaatt cttcggtgca cgtgcgaact tagctaaaac
    11281 attactttac gctattaatg gtggtaaaga tgaaaaatct ggtgcacaag ttggtccaaa
    11341 cttcgaaggt attaacagcg aagtattaga atatgacgaa gtattcaaga aatttgatca
    11401 aatgatggat tggctagcag gtgtttacat taactcatta aatgttattc actacatgca
    11461 cgataaatac agctatgaac gtattgaaat ggcattacat gatacagaaa ttgtacgtac
    11521 aatggcaaca ggtatcgctg gtttatcagt agcagctgac tcattatctg caattaaata
    11581 tgcacaagtt aaaccaattc gtaacgaaga aggtcttgta gtagactttg aaatcgaagg
    11641 cgacttccct aaatacggta acaatgacga ccgtgtagat gatatcgcag ttgatttagt
    11701 agaacgcttt atgactaaat tacgtagtca taaaacatat cgtgattcag aacatacaat
    11761 gagtgtatta acaattactt caaacgttgt atacggtaag aaaactggta acacaccaga
    11821 cggacgtaaa gctggcgaac catttgcacc aggtgcaaac ccaatgcatg gccgtgacca
    11881 aaaaggtgca ttatcttcat taagttctgt agctaagatc ccttacgatt gctgtaaaga
    11941 tggtatttca aatacattca gtatcgtacc aaaatcatta ggtaaagaac cagaagatca
    12001 aaaccgtaac ttaactagta tgttagatgg ttacgcaatg caatgtggtc accacttaaa
    12061 tattaacgta tttaaccgtg aaacattaat agatgcaatg gaacatccag aagaatatcc
    12121 acagttaaca atccgtgtat ctggttacgc tgttaacttc attaaattaa cacgtgaaca
    12181 acaattagat gtaatttctc gtacattcca tgaaagtatg taacaaaatt taaggtggga
    12241 gcactatgct taagggacac ttacattctg tcgaaagttt aggtactgtc gatggaccgg
    12301 gattaagata tatattattc acacaaggat gcttacttag atgcttgtat tgccacaatc
    12361 cagatacttg gaaaattagt gagccatcaa gagaagtcac agttgatgaa atggtgaatg
    12421 aaatattacc atacaaacca tactttgatg catcgggtgg cggtgtaaca gtcagtggtg
    12481 gcgaaccatt gttacaaatg ccattcttag aaaaattatt tgcagaatta aaagaaaatg
    12541 gtgtgcacac ttgcttagac acatcggctg gatgtgctaa tgatacaaaa gcatttcaaa
    12601 ggcattttga agaattacaa aaacatacag acttgatatt attagatata aaacatattg
    12661 ataatgacaa acatattaga ttgacaggaa agcctaatac acacatcctt aacttcgcgc
    12721 gcaaactgtc agatatgaaa caacctgtat ggattcgcca tgtccttgtg cctggttatt
    12781 ctgatgataa agacgattta attaaactag gggaatttat taattctctt gataacgtcg
    12841 aaaagtttga aattctgcca tatcatcagt taggtgttca taagtggaaa acattgggca
    12901 ttgcatatga attagaagat gtcgaagcgc ccgatgatga agctgttaaa gcagcctacc
    12961 gttatgttaa cttcaaaggg aaaattcccg ttgaattata aatacaattc agaccgaaaa
    13021 gaaagcatat gcaacttcaa gagtgaaggg gcatatgctt ctttttcaat tgagtattga
    13081 gtattagcaa ggcgtagtta gtatatgaga caagtccaac aatggttgaa ggtagacgtt
    13141 tttgcaagta gctatgctga taaagaatgt gatgccttgt taaaggtggg gttccaatat
    13201 catcgaatgg gttattaatt gctacttgca tatgaatatg agtcttttca aatttttatt
    13261 gaccctgagt aatgaaaaat attaagatga aacttaatat taaagcaatg cggagcgtga
    13321 ttatgaagag aattagtaaa gatatatggg cagtatttaa attactgtat caaaataaag
    13381 ggcgttttag cattaatgcc ttactattgc aattaatcat gatttttatt agtagtacat
    13441 acttaatttt actatttaat atgatgttaa aagtagctgg gcaaagccaa cttacgatta
    13501 acgattggat ggaaatcgta agtcaccctg ccagtgtgat acttcttatt atattcatat
    13561 taagtgttgc ctttctgatt tatgtagagt tttcattgtt agtttatatg gtttatgccg
    13621 gctttgatcg acagattatt acatttaaat ccatttttaa aaatgccttt gtcaatgtgc
    13681 gtaaactcat aggtgtacca gttgttttct ttgtcattta tttaatgtta atgataccca
    13741 ttgccaacct aggactaagt tcagtattaa caaaaaatat ttacatacct aaatttttaa
    13801 cggaagaact tatgaaaacg acgaaaggta taatcattta cggtaccttt atgattgctg
    13861 tatttatatt aaactttaaa ttaatattta ccttaccgtt aacgatttta aaccgccagt
    13921 cgttatttaa aaatatgaga ctaagttggc aaattacgaa gcgaaataag tttcgacttg
    13981 ttatagaaat agttatattg gaactcatca ttggtgcgat tttaacatta attatttcag
    14041 gagcaacata tcttgctatt tgtgtagatg aagaaggaga taagttttta gtctcctcaa
    14101 ttttatttgt tgtattgaaa agctcattgt tcttttatta tttatttacg aaattatcat
    14161 taatcagtgt gttagtactg cacttaaaac aagagaatgt attagaccaa ccgggcttag
    14221 aatttaaata cccaaaaccg aaacggaagt ctaggttctt tataatttca atggtgcttg
    14281 cagtgacatg ttttgtcggt tataacatgt acttacttta caataatact atcaatacaa
    14341 acatctccat tattggccat cgtggtttcg aagataaagg cgttgaaaat tcgattccgt
    14401 cattgaaagc tgctgcaaaa gcgaatgtcg aatacgttga gttagataca attatgacga
    14461 aagataaaca atttgttgtt agtcatgata acaatttgaa acgtttaaca ggtgttaata
    14521 aaaatatttc tgaatctaat ttcaaagata tcgtcggttt gaaaatgcgt caaaatcgac
    14581 atgaagcaaa acttgtatcc ttagacgaat ttattgaaac ggctaaacaa tcaaatgtga
    14641 agctactagt agagttaaag ccacatggta aagaaccagc agattataca caacgtgtta
    14701 ttgatatttt gaaaaagcat ggtgttgaac atcaatatcg tgtgatgtca ttggattatg
    14761 atgtgatgac taagttgaaa aaagaagcgc catatctcaa gtgtggttat atcattccgt
    14821 tacagtttgg tcattttaaa gaaacaacat tagatttctt tgtcatcgaa gatttttctt
    14881 attcgccaag acttgttaat caagcacact tggaaaataa agaagtctat acttggacca
    14941 ttaacggcga agaagattta acgaaatact tacaaaccaa cgttgatggt attatcacag
    15001 atgacccagc attagctgat cagattaaag aagaaaagaa agacgaaaca tacttcgatc
    15061 gttctataag aattttattt gaataatata aataaagacc tctaaagtta tcaagatgat
    15121 accttcagag gtctttttaa cgttgccatc aatgggatag gcaatcgttt tattcgttta
    15181 taatcatatg acaaggattt ataggataat gtggggtcca aaacacatat atgatttatt
    15241 ggtaagttgc taattgtttt gtgaatgcaa agggttagaa attgaatcgt aaatactttc
    15301 taatctatgt ttcgctttag tcatttgatc caaattttta gtgcgtatag cggattttgc
    15361 aatatagtgc gcagctaaaa tatcgcgttt ttgatacgca tctaaattta ggtacgataa
    15421 tttatttaag tcagtgtttg ctattaattc atgtaattga tctacaagcg cttgatgttg
    15481 atacgtatgt gatgtagttt cagatttgct tgctaattta ataccagtcg tatcaaggag
    15541 cgccgcttta ataccagcaa ctaaatatgt tttgattttc atttgtgttg tcatgctttg
    15601 ttactccttt gatgtacatt aatcaaaa
//
LOCUS       DAPIDM010000051        15619 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000051 DAPIDM010000000
VERSION     DAPIDM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.051"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            64..1029
                     /locus_tag="RJE42_001405"
     CDS             64..1029
                     /locus_tag="RJE42_001405"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV5876830.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            1179..1832
                     /locus_tag="RJE42_001406"
     CDS             1179..1832
                     /locus_tag="RJE42_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDV5876831.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            2143..2715
                     /locus_tag="RJE42_001407"
     CDS             2143..2715
                     /locus_tag="RJE42_001407"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV5876832.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            2715..6221
                     /gene="mfd"
                     /locus_tag="RJE42_001408"
     CDS             2715..6221
                     /gene="mfd"
                     /locus_tag="RJE42_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV5876833.1"
                     /translation="MTILTTLLKEDNHFQGLNQVFGQANTLVTGLSPSAKVTMIAEKN
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            6211..7737
                     /locus_tag="RJE42_001409"
     CDS             6211..7737
                     /locus_tag="RJE42_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDV5876834.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGMLLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NVVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            7737..8930
                     /locus_tag="RJE42_001410"
     CDS             7737..8930
                     /locus_tag="RJE42_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDV5876835.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYETHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEETLNVRTHTLIT
                     QVYSAMVAANLKITLMERYADDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKITTPTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            8927..9190
                     /locus_tag="RJE42_001411"
     CDS             8927..9190
                     /locus_tag="RJE42_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDV5876836.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            9208..9600
                     /locus_tag="RJE42_001412"
     CDS             9208..9600
                     /locus_tag="RJE42_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDV5876837.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            9705..10106
                     /locus_tag="RJE42_001413"
     CDS             9705..10106
                     /locus_tag="RJE42_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDV5876838.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            10286..11581
                     /gene="tilS"
                     /locus_tag="RJE42_001414"
     CDS             10286..11581
                     /gene="tilS"
                     /locus_tag="RJE42_001414"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV5876839.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIIYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIISAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQSFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            11586..12125
                     /gene="hpt"
                     /locus_tag="RJE42_001415"
     CDS             11586..12125
                     /gene="hpt"
                     /locus_tag="RJE42_001415"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV5876840.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGEQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            12383..14476
                     /gene="ftsH"
                     /locus_tag="RJE42_001416"
     CDS             12383..14476
                     /gene="ftsH"
                     /locus_tag="RJE42_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV5876841.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            14705..15586
                     /gene="hslO"
                     /locus_tag="RJE42_001417"
     CDS             14705..15586
                     /gene="hslO"
                     /locus_tag="RJE42_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV5876842.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISAMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
BASE COUNT         5639 a         2139 c         3108 g         4733 t
ORIGIN      
        1 agtaatggag ttacatttta tatataatag taattgcgta agtaaataat tggaggacta
       61 taaatgttaa ataatgaata taagaattcg tcattaaaga ttttttcatt gaaaggaaac
      121 gaagcattag cgcaagaagt tgctgaccaa gtaggaattg aactaggtaa atgttcagtt
      181 aaacgtttta gtgatggaga aattcaaatt aatatcgaag agagtattcg tggttgtgac
      241 gtatttatta ttcaaccaac atcatatcct gtgaatctac atttaatgga attattaatt
      301 atgattgatg cttgtaaacg tgcttctgca gcaacaatca atattgtagt tccatattat
      361 ggatatgcaa gacaagatag aaaagcccgt agccgtgagc caatcactgc taaattagtt
      421 gcaaacttaa tcgaaacagc tggcgcaact cgtatgattg cgttagactt acatgcacca
      481 caaattcaag gattctttga tattccaatt gaccacttaa tgggtgtgcc aattcttgct
      541 aaacatttca aagatgatcc gaatattaac ccagaagaat gtgtcgttgt ttcaccagac
      601 catggcggtg ttacacgtgc acgtaaatta gctgacattt taaaaactcc aattgcaatt
      661 atagataaac gtcgtcctag accaaatgtt gctgaagtga tgaacattgt tggtgagatt
      721 gaaggacgta cggcaattat tattgacgat attattgata cagcaggtac aatcacgtta
      781 gctgcacaag cattaaaaga taaaggtgct aaagaagtat atgcttgttg tacacaccct
      841 gttttatcag gaccggctaa agaacgtatc gaaaattctg ctataaaaga attaatcgta
      901 acaaactcaa ttcatttaga tgaagaccgc aaaccatcta acactaaaga attatctgtt
      961 gctggtttaa tcgcacaagc tatcattcgt gtatacgaaa gagaatcagt tagcgtatta
     1021 tttgactaat atttaaaagg cgtttgacga acatattcca aacgtgtata atagtttcgt
     1081 tcgtgattat acgaataaat aaacacttgc aagcaacgat gatgttgatg ggtaagtgag
     1141 gtgctcgttt tgagcaaaaa tgaaaggtgg aaatgagaat ggcttcatta aagtcaatca
     1201 tccgtcaagg taaacaaaca cgttcagatc ttaaacaatt aagaaaatct ggtaaagtac
     1261 cagcagtagt atacggttac ggtactaaaa acgtgtcagt taaagttgat gaagtagaat
     1321 tcatcaaagt tatccgtgaa gtaggtcgta acggtgttat cgaattaggc gttggttcta
     1381 aaactatcaa agttatggtt gcagactacc aattcgatcc acttaaaaac caaattactc
     1441 acattgactt cttagcaatc aatatgagtg aagaacgtac tgttgaagta ccagttcaat
     1501 tagttggtga agcagtaggc gctaaagaag gcggcgtagt tgaacaacca ttattcaact
     1561 tagaagtaac tgctactcca gacaatattc cagaagcaat cgaagtagac attactgaat
     1621 taaacattaa cgacagctta actgttgcag atgttaaagt aactggcgac ttcaaaatcg
     1681 aaaacgattc agctgaatca gtagtaacag tagttgctcc aactgaagaa ccaactgaag
     1741 aagaaatcga agctatggaa ggcgaacaac aaactgaaga accagaagtt gttggcgaaa
     1801 gcaaagaaga cgaagaaaaa actgaagagt aattttaatc tgttacatta aagtttttat
     1861 actttgttta acaagcactg tgcttatttt aatataagca tggtgctttt tgtgttatta
     1921 taaagcttaa ttaaacttta ttgctttgta ctaaagttaa attaatttta gtgagtaaaa
     1981 gacattaaac tcaacaacga tacatcataa aaattttaat gtacttgatt ttaaaataca
     2041 tacttactaa tctaaagaat aatgataatt gatggcaatg gcggaaaata gatgttgtca
     2101 ttataataat aaatgaaaca attatgttgg aggtaaacac gcatgaaatg tattgtaggt
     2161 ctaggtaata taggtaaacg ttttgaactt acaagacata atatcggctt tgaagtcgtt
     2221 gattatattt tagagaaaaa taatttttca ttagataaac aaaagtttaa aggtgcatat
     2281 actattgaac gaatgaacgg agataaagtg ttatttatcg aaccaatgac aatgatgaat
     2341 ttgtcaggtg aagcagttgc accgattatg gattattaca atgttaatcc agaagattta
     2401 attgtcttat atgatgattt agatttagaa caaggacaag ttcgcttaag acaaaaagga
     2461 agtgcgggcg gtcacaatgg tatgaaatca attattaaaa tgcttggtac agaccaattt
     2521 aaacgtattc gtattggtgt gggaagacca acgaatggta tgacggtacc tgattatgtt
     2581 ttacaacgct tttcaaatga tgaaatggta acgatggaaa aagttatcga acacgcagca
     2641 cgcgcaattg aaaagtttgt tgaaacatca cgatttgacc atgttatgaa tgaatttaat
     2701 ggtgaagtga aataatgaca atattgacaa cgcttttaaa agaagataat cattttcaag
     2761 gccttaatca ggtatttgga caagcaaaca cactagtaac tggtctttcc ccgtcagcta
     2821 aagtgacgat gattgctgaa aaaaatgcac aaagtaatca acagttatta ttaattacca
     2881 ataatttata ccaagcagat aaattagaaa cagatttact tcaatttata gatgctgaag
     2941 aattgtataa gtatcctgtt caagatatta tgaccgaaga gttttcaaca caaagccctc
     3001 aactgatgag tgaacgtatc agaactttaa ctgcgttagc tcaaggtaag aaagggttat
     3061 ttatcgttcc tttaaatggt ttgaaaaagt ggttaactcc tgttgaaatg tggcaaaatc
     3121 accaaatgac attgcgtgtt ggtgaggata tcgatgtgga ccaatttctt aacaaattag
     3181 ttaatatggg gtacaagcgg gaatccgtgg tatcgcatat tggtgaattc tcattgcgag
     3241 gaggtattat cgatatcttt ccgctaattg gggaaccaat cagaattgag ctatttgata
     3301 ccgaaattga ttctattcgg gattttgatg ttgaaacgca gcgttccaaa gataatattg
     3361 aagaagtcga tataacaact gcaagtgatt atatcattac tgaagaagtg atcagccacc
     3421 ttaaagaaga gttaaaaact gcatatgaaa atacaagacc caaaatagat aaatcagtgc
     3481 gcaatgatct gaaagaaacg tatgaaagct ttaaattatt cgaaagtaca tactttgatc
     3541 atcaaatact acgtcgttta gtagcattta tgtatgaaac accttcgaca attattgatt
     3601 atttccaaaa agatgcaatc attgcggttg atgaatttaa tcgtattaaa gaaactgaag
     3661 aaagtttaac agtagagtct gattcgttta ttagcaacat tattgaaagt ggtaatggat
     3721 ttataggaca aagttttata aaatatgatg attttgaaac gttgattgaa ggctatcctg
     3781 tcacttattt ttcattattc gctacaacaa tgccgataaa actaaatcat attattaaat
     3841 tttcatgtaa acctgtccaa caattttatg ggcaatatga cattatgcgt tctgaatttc
     3901 aacgatatgt taatcaaaac tatcatatcg tggttttggt cgaaaccgaa actaaagttg
     3961 aacgtatgca agcgatgtta agtgaaatgc atattccatc aataacaaaa ttgcatcgct
     4021 caatgtcatc ggggcaagca gtgattattg aaggcagttt atctgaagga tttgaactac
     4081 ctgatatggg attagttgtc attactgaac gtgagctttt taaatcaaaa cagaaaaagc
     4141 aacgaaaacg tacgaaagct atttcaaatg ctgaaaaaat taagtcttac caagatttaa
     4201 atgtgggaga ttatattgtt catgtgcatc atggtgttgg tagatattta ggtgttgaga
     4261 cgctcgaagt agggcaaaca catcgtgatt atattaaatt acaatataaa ggtacggatc
     4321 aactatttgt tccagtagat caaatggatc aagttcaaaa atatgtagct tcggaagata
     4381 agacgccaaa attaaataaa ctcggtggca gtgaatggaa aaaaacaaaa gctaaagttc
     4441 aacaaagtgt tgaagatatt gctgaagagt tgattgattt atataaagaa agagaaatgg
     4501 cagaaggtta tcaatatggg gaggacacag ctgagcaaac aacatttgaa ttagattttc
     4561 catatgaact tacgcctgac caagcgaaat ctatcgatga aattaaggat gacatgcaaa
     4621 aatcgcgtcc aatggatcgc ttgctatgtg gtgatgttgg ttatggtaaa accgaagttg
     4681 cagtgagagc agcattcaaa gctgtaatgg aaggaaagca ggttgcattt ttagttccta
     4741 caactatttt agctcagcaa cattatgaga cgttaattga gcgtatgcag gattttcctg
     4801 ttgaaattca attaatgagt cgttttagaa cgccaaaaga gataaaacaa actaaggaag
     4861 gacttaaaac tgggtttgtt gacatagttg ttggtacaca caaattactt agtaaagata
     4921 tacagtataa agatttaggg ctgttgattg tagatgaaga acaacgattt ggtgtacgcc
     4981 ataaagagcg tattaaaaca ttaaaacata atgtagatgt actaacattg actgcaaccc
     5041 caatacctag aacattgcat atgagtatgc taggtgtgcg cgatttgtca gtgattgaaa
     5101 cgccgccaga aaatcgtttc ccagttcaaa catatgtatt agaacagaac atgagtttta
     5161 tcaaagaagc tttagaaaga gaactatccc gtgatggcca agtattttat ctttataata
     5221 aagtgcaatc catttatgaa aaacgagaac aactccagat gttaatgcca gatgctaaca
     5281 ttgcagttgc tcatggacaa atgacagagc gtgatttaga agaaacgatg ttaagtttta
     5341 tcaataatga atatgatatt ttagtaacga cgacgattat tgaaacaggt gtcgatgtcc
     5401 caaatgcaaa tactttgatc attgaagatg cagatcgctt tggattgagt cagttgtatc
     5461 aattaagagg tcgtgttggt cgttcaagtc gtattggtta tgcatacttc ttacatccag
     5521 caaataaggt actaactgag actgcagaag atcgattaca agcgattaaa gaatttacgg
     5581 agttaggctc aggatttaag attgcgatgc gtgatttgaa cattcgtggt gctggtaatt
     5641 tgttaggtaa acaacagcac ggctttattg atacagttgg atttgatttg tacagtcaaa
     5701 tgttagaaga agctgtaaat gaaaaacgtg gtattaagga accagaatct gaggtgccag
     5761 aagtcgaagt tgatttaaac ttggatgcat atttgccaac agaatatatt gcaaatgaac
     5821 aagctaaaat tgaaatttat aaaaagctac gaaaaactga aacatttgat caaattatcg
     5881 acattaaaga tgaattaatt gatcgtttca atgattatcc tgttgaagta gcacgtttgc
     5941 ttgatatagt ggaaataaaa gtacacgcat tacattcagg tatcacgttg attaaagata
     6001 aagggaaaat aattgatatt catttatctg taaaagccac tgaaaatatt gatggcgaag
     6061 tgctgttcaa agcaacacaa cctttaggta gaacaatgaa ggttggtgtt caaaataatg
     6121 caatgacaat tactttaacg aaacaaaatc aatggcttga tagtttgaag tttttagtta
     6181 agtgcattga agaaagtatg agaatcagtg atgaagcata aagaagcatt taatggcgtt
     6241 gtcgtgttaa ctgctgcatt aattgtcatt aaaattctga gtgctgtata tcgaattcca
     6301 tatcaaaata tattaggcga tacaggtttg tatgcatatc aacaagtgta tccaattgta
     6361 gcattaggaa tgatattatc gatgaatgcc attcctagtg caattacaca aaatataggg
     6421 aagtatcata gtgacgaagc atatgcaaaa gcagtcgctt atatacaatt agttggtatg
     6481 ttattattta ttgctatttt tgtgtttgcg aacaacattg cacatatgat gggtgatagc
     6541 catttaacac ctatgattca agctgcaagt ttaagcttta tatttatagg tatgcttggc
     6601 gtgttaagag gttattatca atctgcaaat aatatgacag ttccggctat ttcccaggtt
     6661 atagaacaag ttatacgagt aggtattatc attgttacta ttgttatttt tgtagataga
     6721 ggttggacga tatatgaagc gggaacaatt gctattttag catcaacgat aggtttttta
     6781 ggttcttcaa tttatttagt agcgcaccga ccttttaagt ttaaaatggt aaataacact
     6841 gcaaagattg tttggaaaca gttcgcactt tcggttttga ttttcgctat cagtcaatta
     6901 atcgtaattt tatggcaagt gattgatagt gttactatta ttaagtcact tcaagcgata
     6961 cgcgtgccat tcgatgttgc cataactgaa aaaggagtct atgaccgtgg tgcatcattt
     7021 attcagatgg gattgattgt aactacaaca tttagttttg cgctcattcc tctgttaagt
     7081 gacgcaatca aaatgaacaa tcaggtactt atgaatcgtt atgcaaatgc gtcattaaag
     7141 attacgattt taataagtac agcagcggga ataggattaa ttaatttatt gcctttaatg
     7201 aacggtgtat tttttaagac gaatgattta accttaacgt taagcgttta tatgattacg
     7261 gtcatttgtg tatcgttaat tatgatggat atggcattat tacaagctca acatgctgtg
     7321 agacctattt ttgtcggtat gacggcagga ttggttatta aatttatact taatatcatt
     7381 ttgattcgtt taagtggcat tattggtgcg agcattagta ctgttgtatc attaattata
     7441 ttcggtacga ttatccatat tgctgtcacg agaaaatacc atttacatgc gatgagacga
     7501 ttttttatca atgttgtttt aggtatggta tttatgtcga ttgttgtaca atgcgtgtta
     7561 aacgtagtga caacacacgg tagaatcact ggactcattg aattattatg tgcagcagta
     7621 ttaggtatca ttgcattgtt tttctatatt tttagattta atgttttgac atataaagag
     7681 ttaacttatt taccatttgg ttcaaagttg tatcaaatta agaaaggaag acgttgatgg
     7741 cacataccat tacgattgtt ggcttaggaa actatggcat tgatgatttg ccgctaggga
     7801 tatataaatt tttaaagaca caagataaag tttatgcaag aacgttagat catccagtta
     7861 tagaatcatt gcaagatgaa ttaacatttc agagttttga ccatgtttat gaaacacatg
     7921 accaatttga agatgtctat aatgatattg tggcgcaatt ggttgaagct gctaatgaaa
     7981 aagatattgt ctatgcggtt ccgggtcatc ctagagttgc tgagacaact acagtgaaat
     8041 tactggcttt agcaaaggac aatactgata tagatgtgaa agttttaggt gggaaaagct
     8101 ttattgatga tgtttttgaa gcagttaatg tagatccaaa tgatggcttc acactgttag
     8161 atgcgacatc attacaagaa gaaacactta atgttagaac gcatacattg attacgcaag
     8221 tttatagtgc aatggttgct gctaatttga aaatcacttt aatggaacga tatgctgatg
     8281 attatcctgt tcaaattgtc actggtgcac gaagcgatgg tgcggataac gttgtgacat
     8341 gcccattata tgaattggat catgatgaaa atgcattcaa taatttgacg agtgtattcg
     8401 taccaaaaat tacaacaccg acatatttgt atcatgactt tgattttgcg acggaagtga
     8461 ttgatacttt agttgatgaa gataaaggtt gtccatggga taaagtgcaa acgcatgaaa
     8521 cgcttaaacg ttatttactt gaagaaacat ttgaattgtt cgaagctatt gacaatgaag
     8581 atgattggca tatgattgaa gaactaggag atattttatt acaagtgtta ttgcatacta
     8641 gtattggtaa aaaagaaggg tatatcgaca ttaaagaagt gattacaagt cttaatgcta
     8701 aaatgattcg tagacaccca cacatatttg gtgatgctaa tgctgaaact atcgatgact
     8761 taaaagaaat ttggtctaag gcgaaagatg ctgaaggtaa acagccaaga gttaaatttg
     8821 aaaaagtatt tgcagagcat tttttaaatt tatatgagaa gacgaaggat aagtcatttg
     8881 atgaggccgc gttaaagcag tggctagaaa aaggggagag taatacatga gattagataa
     8941 atatttaaaa gtatcacggt taataaagcg acgtacgcta gcaaaagagg taagtgatca
     9001 aggtagaatt acaataaatg gtaatgttgc taaagctgga tcggatgtta aagttgaaga
     9061 tgtgctgacg attcgctttg gtcaaaaatt agtaacagtt aaagtaactg cattaaatga
     9121 acatgcatct aaagataacg cgaagggcat gtatgaaatc attgaagagc gtcgacttga
     9181 agaagcgtaa attggaggtg acaagcaatg aaaaataaag tagaacatat agaaaatcag
     9241 tacacgtcgc aagagaataa gaaaaaacaa cgtcaaaaaa tgaaaatgcg tgttgttcgt
     9301 aggcgtatta cagtatttgc gggcgtatta cttgcgataa ttgttgtttt atcaatcttg
     9361 cttgttgtcc aaaaacatcg caatgatatt gatgcacagg agcgaaaagc gaaagaagca
     9421 cagtttcaaa agcaacaaaa tgaagaaatt gcgttaaaag aaaagttgaa taatctgaat
     9481 gacaaagatt atattgaaaa aattgcgcgt gatgattatt acttaagcaa caaaggtgaa
     9541 gtgattttta ggttgccaga agacaaagat tcgtctagct caaaatcttc gaaaaaataa
     9601 atccaaattg attcaaaatt atccgagtat agacattgtg aaaaaatcca aacaaggata
     9661 taataaggga aaatcgaatc aaatcgggag gatttattta acatatgtca atcgaagttg
     9721 gaaataagct taaaggtaaa gtcactggta ttaaaaagtt tggtgcattc gtagaattac
     9781 ctgaaggaaa aagtggttta gttcacatta gtgaagtcgc agataattat gttgaaaacg
     9841 tagaagaaca cctttctgtt ggtgatgaag tagacgtaaa agtattatct attgctgatg
     9901 atggaaaaat tagtctttca attaagaaag ctaaagaccg tccacgtaga caacatacga
     9961 gtaaaccaag tcatcaaaaa ccagtgcaaa aagccgaaga ttttgaaaag aaattaagca
    10021 atttcttaaa agatagtgaa gataaattaa cttcaatcaa acgtcaaaca gaatctagac
    10081 gcggtggcaa aggttctaga cgttaattaa aataaataaa gactgtttcg ataaggaata
    10141 tatttagaat gatgcgtatc gaataatcga ttgcagcgtt agacaatcta agactgtttc
    10201 ttaaataagg agcagtctct tttatttgta atgatataac taagacttat accatttttg
    10261 aaaattgtaa aagtgaggtg atgttatgca gttaaatagt aatggttggc atgttgatga
    10321 ccatattgtt gtcgctgttt ctacaggtat tgatagtatg tgtttattgt atcaactact
    10381 aaatgattat aaagatagtt atagaaaact aacatgttta catgtcaatc atggcgttag
    10441 gtcagcttca atcgaggaag ccagattttt agaagcatac tgcgaacgtc atcacatcga
    10501 tttacatatc aaaaagttgg atttgtcgca tagtctcgac cgaaataaca gcattcagaa
    10561 tgaagctcga attaaacgtt acgaatggtt tgatgaaatg atgaatgtat tagaagcgga
    10621 tgtattgcta acggcgcatc atttggacga tcaattagaa actattattt atcgcatttt
    10681 taatgggaaa tcaacgcgta ataaactagg atttgatgag ttatcgaagc gaaaaggtta
    10741 tcagatttat agaccacttt tagctgtctc taaaaaagaa ataaaacaat tccaagagag
    10801 atatcatata ccatattttg aagatgaatc taataaagat aacaaatatg ttagaaatga
    10861 tattcgtaat agaattattt cagctattga tgaaaataat caacttaaag tatcgcattt
    10921 attaaaatta aaacaatggc atgatgaaca atatgatatt ttgcaatatt cagctaaaca
    10981 atttattcaa gaatttgtga agtttgatga acagtcaaaa tatttagagg tttctagaca
    11041 atcttttaat aacttgccaa actcattaaa gatggttgtg ttggattgcc tattatcaaa
    11101 gtattatgag ttgtttaata ttagtgctaa aacatacgaa gagtggttta aacaatttag
    11161 tagtaagaaa gcacaattca gtattaatct cacggataaa tggataattc aaatcgcata
    11221 tggtaaatta ataataatgg ctaaaaataa tggcgataca tattttagag ttcaaactat
    11281 tgaaaagcca ggtaattata tttttaacaa atatcgatta gagatacatt ctaatttacc
    11341 aaaatgttta tttccgctta cagtgagaac acgacaaagt ggcgatacat ttaaactgaa
    11401 tgggcgcgat ggttataaga aagtgaatcg tctgtttata gattgtaaag tacaacagtg
    11461 ggttcgggat caaatgccaa tcgtattgga taaacaacag cgcattattg cggtaggaga
    11521 tttatatcaa caacaaacaa taaaacaatg gattataatt agtaaaaatg gagatgaata
    11581 gcgttatgca taatgatttg aaagaagtat tgttaactga agaagatatt caaaatatct
    11641 gtaaggaatt gggagaacaa ttaacaaagg attatcaagg taaaccatta gtatgcgtgg
    11701 gtatcttaaa aggctcagca atgtttatgt cagatttaat taaacgaatt gatacccatt
    11761 tatcaattga tttcatggat gtttctagtt atcacggagg cactgagtca actggtgaag
    11821 ttcaaatcat taaagattta ggttcttcta ttgaaaataa agacgtatta attattgaag
    11881 atatcttaga gactggtact acacttaagt caattactga attattacaa tctagaaaag
    11941 ttaattcatt agaaatagtt actttattag ataaaccaaa ccgtcgtaaa gcggacattg
    12001 aagctaagta tgtaggtaaa aaaataccag atgaatttgt tgttggttac ggtttagatt
    12061 atcgtgaatt ataccgaaac ttaccatata tcggtacgtt aaaacctgaa gtgtattcaa
    12121 attaattttt taatcaattt cagttattat tactatgcgt ttgagaaata atagtgtaga
    12181 ctcaaaaata tgaaaaatgt atttcatata tatttaattt tagacaagac atatgtctta
    12241 aaaagttgaa aaatatagag attgataaaa ctaacacagg gtgtgaatga cattgatgtt
    12301 aagctcaatt actagcttat aaaacatgtc atatgttaca atttttgtta gttttattat
    12361 gggaagtagg aggaaatgac gcatgcagaa agcttttcgc aatgtgctag ttatcgtaat
    12421 aataggcgtt attatttttg gtctattttc atatttaaac ggtaatggaa atatgccgaa
    12481 acagcttaca tataatcaat ttactgagaa gttggaaaaa ggtgacctta aaactttaga
    12541 aatccaacca caacaaaatg tctatatggt aagtggtaaa acgaaaaatg atgaagacta
    12601 ttcatcaact attttatata acaacgaaaa agaattacaa aaaattactg atgctgctaa
    12661 aaagcaaaac ggtgtaaaat taacgattaa agaagaagaa aaacaaagtg tctttgtgag
    12721 tatactttca acattaattc cagttgtagt catagcgtta ttatttattt tcttcctaag
    12781 ccaagcacaa ggtggcggta gtggcggtcg tatgatgaac tttggtaaat ctaaagcaaa
    12841 aatgtacgat aataataaac gtcgtgttcg tttctctgat gtagcagggg cagatgaaga
    12901 aaaacaagaa ttaattgaaa ttgttgattt cttgaaagat aataaaaaat tcaaagaaat
    12961 gggatctagg attcctaaag gtgtcttact tgttggacct ccaggtactg gtaaaacatt
    13021 acttgctaga gcggttgcag gtgaagcagg cgcaccattc ttctctatta gtggttcaga
    13081 ctttgtagag atgtttgttg gtgttggtgc gagccgtgtt cgtgacttat tcgataatgc
    13141 taagaaaaac gcgccttgta tcatctttat cgatgagatt gatgctgttg gtcgtcaacg
    13201 tggtgcaggt gttggtggcg gtcatgatga acgtgaacaa accctaaacc aattattagt
    13261 tgaaatggat ggattcggtg aaaatgaagg aatcattatg atagctgcta caaaccgtcc
    13321 tgatatcctt gacccagcct tattacgtcc aggtcgtttt gatagacaaa ttcaagttgg
    13381 tcgtccagat gtgaaaggcc gtgaagcaat tcttcatgtt catgctaaaa ataaaccact
    13441 tgatgaaacg gttgatttaa aagcaatttc acaacgtaca cctggtttct caggtgctga
    13501 tttagagaac ttattaaatg aagcatcttt aattgctgta cgtgaaggta aaaagaaaat
    13561 tgacatgaga gatatcgaag aggcaacgga tagagttata gccggacctg ctaagaaatc
    13621 tcgagttatt tctaagaaag aacgtaatat tgttgctcat cacgaagctg gtcatacaat
    13681 tatcggtatg gtacttgatg aggcagaagt agtgcataaa gttactatcg ttccacgtgg
    13741 acaagctggt ggttatgcaa tgatgctacc taaacaagat cgtttcttaa tgactgaaca
    13801 agagttatta gataaaatct gtggtttact tggtggacgt gtatcagaag atattaactt
    13861 taacgaagta tcaacaggtg cttcaaatga cttcgaacgt gcaacacaaa tcgcacgctc
    13921 aatggttacg caatatggta tgagtaaaaa attaggacca ttacagttcg gtcatagcaa
    13981 tggtcaagta ttcttaggta aagatatgca aggtgagcct aattattcaa gccaaatcgc
    14041 atatgaaatt gataaagaag ttcaacgaat cgttaaagaa caatacgaac gttgtaaaca
    14101 aattttatta gagcacaaag aacaattaat tttaattgct gaaacattat taacagaaga
    14161 aacattagtt gctgaacaaa ttcaatcatt attctacgaa ggtaaattac ctgaaattga
    14221 ttatgatgca gctaaagttg ttaaagatga agattctgaa tttaatgatg gtaaattcgg
    14281 taaatcttat gaagagattc gtaaagagca attagaagat ggacaacgtg acgaaagtga
    14341 agatcgtaaa gaagaaaaag atattgctga ggataaaaaa gaagctgata aatctgatga
    14401 aaaagatgaa ccagcacatc gacaagcccc aaatatcgaa aaaccttacg atccaaatca
    14461 cccagacaat aaataatcga ttatattcag tacctctttc tatgataaag ttatagaaag
    14521 aggtactttt atgatttaag gaatacacga ttacttacta agtaatggta ttatggcacg
    14581 aataattaat aatcaaacaa gttgcttcat tatttagaat gatgggtgta gaataagtac
    14641 aattgttgca ttttatgaag taaagtaatt tttaaaatat agagtaatag aggagattga
    14701 aataatgaca cacgattata ttgttaaagc attagcattt gatggggaga ttagggctta
    14761 tgctgctttg acaactgaaa ctgttcaaga agcacaaacg agacattata catggccgac
    14821 agcatctgct gcaatgggaa gaacaatgac agcaacagct atgatgggcg caatgttgaa
    14881 aggtgatcaa aaattaacag tcactgtaga tggccaagga cctattggac gaattattgc
    14941 cgatgcaaat gctaaaggcg aggtgcgtgc ttatgtagac catccacaaa ctcattttcc
    15001 attaaatgag caaggtaaac ttgatgtaag acgagcagta gggacaaatg gatctattat
    15061 ggttgttaaa gacgttggaa tgaaagacta tttctctgga gcaagtccaa ttgtttcagg
    15121 agaacttggt gaagatttta cttattatta tgctacaagt gaacaaacac cttcatcggt
    15181 aggtcttggt gtattggtaa atcctgataa tacgattaaa gcagcaggag gatttatcat
    15241 tcaagttatg ccaggtgcca aagatgaaac aatttcaaaa ttagaaaaag caattagtgc
    15301 aatgacacca gtttctaaat taattgagca aggattaacg ccagaaggat tactaaacga
    15361 aatcttaggt gaagaccatg tgcaaatttt agagaaaatg cctgttcaat ttgaatgtaa
    15421 ttgtagtcat gagaaatttt taaatgctat taaaggattg ggcgaggctg agattcaaaa
    15481 tatgattaaa gaagatcatg gtgctgaagc agtatgtcat ttctgtggaa ataaatataa
    15541 atatactgaa gaagaattaa acgtgttgct agaaagttta gcgtaattta atttaaatca
    15601 atacgctaaa atgtttatt
//
LOCUS       DAPIDM010000052        15580 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000052 DAPIDM010000000
VERSION     DAPIDM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.052"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..921
                     /locus_tag="RJE42_001418"
     CDS             <1..921
                     /locus_tag="RJE42_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDV5876843.1"
                     /translation="TRLSDELDKLKQQRIELNEQIDAQEATLQVCHQDILAIENHYQD
                     IKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKKVKLMKMSIDELG
                     PVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVTERFKETFHAIQG
                     HFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLLSGGERALTAIAL
                     LFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVITHRKGTMEFADR
                     LYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            921..2171
                     /gene="ftsY"
                     /locus_tag="RJE42_001419"
     CDS             921..2171
                     /gene="ftsY"
                     /locus_tag="RJE42_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV5876844.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVNGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            2158..2490
                     /locus_tag="RJE42_001420"
     CDS             2158..2490
                     /locus_tag="RJE42_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDV5876845.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            2516..3883
                     /gene="ffh"
                     /locus_tag="RJE42_001421"
     CDS             2516..3883
                     /gene="ffh"
                     /locus_tag="RJE42_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV5876846.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            4318..4593
                     /gene="rpsP"
                     /locus_tag="RJE42_001422"
     CDS             4318..4593
                     /gene="rpsP"
                     /locus_tag="RJE42_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV5876847.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            4781..5284
                     /gene="rimM"
                     /locus_tag="RJE42_001423"
     CDS             4781..5284
                     /gene="rimM"
                     /locus_tag="RJE42_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV5876848.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLDENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            5284..6021
                     /gene="trmD"
                     /locus_tag="RJE42_001424"
     CDS             5284..6021
                     /gene="trmD"
                     /locus_tag="RJE42_001424"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV5876849.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            6124..6474
                     /gene="rplS"
                     /locus_tag="RJE42_001425"
     CDS             6124..6474
                     /gene="rplS"
                     /locus_tag="RJE42_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV5876850.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(6716..9322)
                     /gene="yfhO"
                     /locus_tag="RJE42_001426"
     CDS             complement(6716..9322)
                     /gene="yfhO"
                     /locus_tag="RJE42_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDV5876851.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKFICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSISTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQYKDLYLEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKSSDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGIMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            9723..10607
                     /gene="ylqF"
                     /locus_tag="RJE42_001427"
     CDS             9723..10607
                     /gene="ylqF"
                     /locus_tag="RJE42_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDV5876852.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            10591..11358
                     /locus_tag="RJE42_001428"
     CDS             10591..11358
                     /locus_tag="RJE42_001428"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV5876853.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKNYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            11467..12633
                     /gene="sucC"
                     /locus_tag="RJE42_001429"
     CDS             11467..12633
                     /gene="sucC"
                     /locus_tag="RJE42_001429"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV5876854.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            12655..13563
                     /gene="sucD"
                     /locus_tag="RJE42_001430"
     CDS             12655..13563
                     /gene="sucD"
                     /locus_tag="RJE42_001430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV5876855.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVESVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            13790..14002
                     /locus_tag="RJE42_001431"
     CDS             13790..14002
                     /locus_tag="RJE42_001431"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HDV5876856.1"
                     /translation="MNKQQSKVRYSIRKVSIGTFLNGDYDGKLMKFQSLDQNWNNGGW
                     RKAEVAHKVVHNYENDMIFIRPLKKA"
     gene            14030..15273
                     /gene="fmhC"
                     /locus_tag="RJE42_001432"
                     /pseudo
     CDS             14030..15273
                     /gene="fmhC"
                     /locus_tag="RJE42_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
     gene            15444..>15580
                     /locus_tag="RJE42_001433"
     CDS             15444..>15580
                     /locus_tag="RJE42_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV5876857.1"
                     /translation="MIRLFLLKLYWAHFSTKQIHQFLMAYPNVIKEGGRKKDSYLCEW
                     V"
BASE COUNT         5780 a         2060 c         2954 g         4786 t
ORIGIN      
        1 acacgcttat ctgatgaatt agataaattg aaacaacaac gtattgagtt gaatgaacaa
       61 atcgatgcgc aagaagctac actacaagtt tgtcaccaag atattttagc tatcgaaaat
      121 cactaccaag atattaaagc tgaacaatca aagctagatg tattaattca tcatgcgata
      181 gatcatttaa atgatgaata tcaattgact gttgaacgtg caaaatctga atatacgagt
      241 gatgaatcga ttgacgcatt acgtaaaaaa gttaaattaa tgaagatgtc gattgatgaa
      301 ctaggtcctg taaacttaaa tgcaattgaa caatttgaag agttaaatga acgttataca
      361 tttttaagtg agcaacgtac agatcttcgt aaagctaaag aaacattaga gcaaattata
      421 agtgaaatgg atcaagaggt tactgaaaga tttaaagaaa ctttccatgc tattcaagga
      481 cattttacag ctgtgttcaa acaattgttt ggtggaggcg atgcagaatt gcaattaact
      541 gaagccgatt atttaacagc tggtattgat attgtggtac aaccaccggg taaaaagttg
      601 caacatttat cgttactaag tggtggtgag cgtgcattaa ctgctattgc tttactattt
      661 gcaattttaa aagtaagatc tgcacctttt gttatattag atgaggttga agctgcatta
      721 gatgaagcga atgttattag atacgcaaaa tatttaaatg agttatcaga cgaaacacaa
      781 ttcattgtta ttacacaccg taaaggaaca atggaatttg cagataggtt atacggtgta
      841 acaatgcaag aatcaggtgt tactaaactt gtgagtgtga atttaaatac aatagatgat
      901 gtgttgaagg aggagcaata atgagctttt ttaaacgctt aaaagataag tttgcaacaa
      961 ataaagaaaa tgaagaagtt aaatccttaa cagaagaaca aggtcaagac aaattagaag
     1021 atacacattc tgaaggttca acgcaggacg taaatgattt agcagaaaat gctgaagtga
     1081 aaaagaagcc acgcaagttg agtgaagcgg attttgatga cgatggctta atatcaattg
     1141 aagattttga agaaattgaa gctcaaaaaa tgggtgctaa atttaaagca ggactcgaaa
     1201 aatctcgtca aaatttccaa gaacaattaa ataatttgat agcgagatat cgtaaagtag
     1261 atgaagactt ttttgaagct ttagaagaaa tgctaatcac tgcagacgtc ggttttaata
     1321 cagtgatgac gttaactgaa gaattacgta tggaagcaca acgacgtaat attcaagata
     1381 ctgaagattt gcgtgaagtc attgttgaaa aaatcgtaga gatttaccat caagaagatg
     1441 ataattcaga agctatgaac ttagaagatg gtcgtttaaa tgtcatttta atggtcggtg
     1501 tgaatggtgt tggtaaaaca acaacaattg gaaaattagc ttaccgatat aaaatggaag
     1561 gtaaaaaagt aatgttagct gcgggcgata cttttagagc gggtgctatt gatcaattga
     1621 aagtttgggg cgaacgtgtt ggtgtagatg taattagcca aagtgaaggt tctgatccag
     1681 ctgctgttat gtatgatgcg attaatgccg ctaaaaacaa aggtgttgat attttaatct
     1741 gtgataccgc tggacgttta caaaataaaa caaatctaat gcaagaatta gaaaaagtta
     1801 agcgtgtaat taatcgagca gtgccagatg cgcctcatga agcattacta tgtttagatg
     1861 ctacaactgg tcagaatgcg ttgtcacaag ctagaaactt taaagaagta acaaatgtta
     1921 caggtattgt attaacgaaa ttagatggta cagccaaagg tggcatcgta ttagccattc
     1981 gtaatgaatt gcacattcca gttaaatatg taggtttagg tgagcaatta gatgacttac
     2041 aaccatttaa ccctgaaagt tatgtcaacg gcttattcgc tgatatgatt gaacaaaatg
     2101 aagaaataac aacagttgaa aatgatcaaa ttgtaacaga agaaaaggac gataatcatg
     2161 ggtcaaaatg atttagttaa aacgttacga atgaattatt tgtttgattt ttatcaatcc
     2221 ttattgacga ataaacaacg taattatttg gaattatttt atcttgaaga ttattcttta
     2281 agtgaaatcg cagatacttt taatgtgagt agacaagcag tttatgataa tataagaaga
     2341 actggcgatt tagttgaaga ttatgaaaag aaattggaat tataccagaa atttgagcaa
     2401 cgccgagaaa tatatgatga aatgaaacaa catttaagta atccagaaca aatacaacgt
     2461 tatattcaac aattagaaga cttagaatag atataaattg agggaggaca tcgatatggc
     2521 atttgaaggg ttatcagaac gcttgcaagc gacgatgcaa aaaatgcgtg gtaagggtaa
     2581 acttactgaa gctgatataa agataatgat gcgtgaagta agattagcgt tacttgaggc
     2641 tgacgtaaac tttaaagtgg taaaagaatt tattaaaaca gtatcagaac gcgcattagg
     2701 ttccgatgta atgcaatcat taacaccagg gcaacaagtt attaaaatag ttcaagatga
     2761 attaacgaag ttgatgggtg gagaaaatac atcgattaat atgtcaaata aaccacctac
     2821 tgttgttatg atggttggtt tacaaggtgc tggtaaaaca acaactgcag gtaaattagc
     2881 attattgatg cgtaaaaaat acaacaaaaa acctatgtta gttgcagcag atatttatcg
     2941 tccagcagcg ataaatcaat tacaaacagt agggaaacaa attgatattc ctgtatacag
     3001 tgaaggagat caagtaaagc cacaacaaat tgtaactaat gcattaaaac atgctaaaga
     3061 agaacattta gactttgtaa tcattgatac agcaggtcga ttacacatcg atgaagcatt
     3121 gatgaacgaa ttaaaagaag taaaagacat tgctaaacca aacgaaatta tgttagttgt
     3181 cgattcaatg acgggtcaag atgctgtcaa tgttgcagaa tcttttgacg atcaacttga
     3241 tgtcacaggt gttaccttaa ctaaattaga tggtgataca cgtggtggtg cagctttatc
     3301 tattcgttcg gtgacacaaa aaccaattaa atttgttggt atgagtgaaa agttagatgg
     3361 tttagagcta ttccatcctg aacgtatggc atcacgtatt ttaggtatgg gtgatgtgtt
     3421 aagtttaatt gaaaaagcgc aacaagatgt ggatcaagaa aaagcaaaag atttagagaa
     3481 aaagatgcgt gagtcatcgt ttactttaga tgatttttta gaacaacttg atcaggtgaa
     3541 aaatctagga ccactggatg atattatgaa aatgattcca ggtatgaata aaatgaaagg
     3601 gctagataag cttaatatga gtgaaaagca aattgatcat attaaagcga ttatccagtc
     3661 aatgacgccg gctgaaagaa acaatccaga cacattgaat gtatcacgta aaaagcgtat
     3721 tgctaaaggg tctggtcgtt cattacaaga agtcaatcgt ttgatgaaac aatttaacga
     3781 tatgaagaaa atgatgaaac aattcactgg tggcggtaaa ggtaaaaaag gtaaacgcaa
     3841 tcaaatgcaa aatatgttaa aaggtatgaa tttaccgttt taatgtttaa tataacacgc
     3901 tgatttttgt tgcaatgaga tgtgcatccc gaaagttata ctaaagaata taactttatg
     3961 gggtgcatat ttttagatac acaataataa ttaaaaactg aaattgcgga tttgctagaa
     4021 catgaggtta ttgttgctaa tattaataag ttatattggt ggaacatgtg cgttagaagt
     4081 ggtaccattc atgtccttac gagataaatt aacttaacac gtaggtggtg aaatttacta
     4141 tgaattttta taacctatta acagtctaat ttagtaatca catagttatt attgtaagaa
     4201 aaaggttcaa taattttcct ggtaaagaaa aaactcttta caaacattca cacacctgtt
     4261 aatattattt cttgtagaaa ataaaaatta aaacatgact taaaggagat tttataaatg
     4321 gcagttaaaa ttcgtttaac acgtttaggt tcaaaaagaa atccattcta tcgtatcgta
     4381 gtagcagatg ctcgttctcc acgtgacgga cgtatcatcg aacaaatcgg tacttataac
     4441 ccaacgagcg ctaatgctcc agaaattaaa gttgacgaag cgttagcttt aaaatggtta
     4501 aatgatggtg cgaaaccaac tgatacagtt cacaatatct tatcaaaaga aggtattatg
     4561 aaaaaatttg acgaacaaaa gaaagctaag taatttagcg taaaattgtt ctaacaataa
     4621 gaataactcg tttacactga cagttattac tcaatgatac gttgggaata tcacatgtta
     4681 gtaatataga acgtattggg taccataatg gtgccctttt tctttgaatt attttcaatt
     4741 aaaatagaag tgttcaaagc atagagttgg aggtaataga atgagagttg aagttggtca
     4801 aattgttaac acacatggta ttaaaggtga aattaaagta aaatccaatt cagactttac
     4861 agacgttcgt tttcaacccg gtcaagtgct gacagttgtg cataacaata acgaccttga
     4921 atatactgtt aagtcacata gagtgcataa agggcttcat atgctcacat ttgaaggtat
     4981 taataatatt aatgatattg agcatctaaa agggagttct atttatcaag agcgtgatca
     5041 tgaagatatc gtacttgatg aaaatgaatt ttattattca gatattatag gatgtacagt
     5101 ttttgatgat caagaaacac caataggtcg tgtaattaat atatttgaaa caggtgcgaa
     5161 tgatgtgtgg gtgattaaag gatctaaaga atatttgatt ccttatattg ctgatgttgt
     5221 aaaagaagtg gatgttgaaa ataaaaaaat tatcatcacg ccaatggaag gattgttgga
     5281 ttaatgaaaa ttgattattt aactttattt cctgaaatgt ttgatggtgt tttaaatcat
     5341 tcaattatga aacgtgccca agaaaacaat aaattacaaa tcaatacggt taattttaga
     5401 gattatgcaa ttaacaagca caaccaagta gatgattatc cgtatggtgg cggacaaggt
     5461 atggtgttaa agcctgaacc tgtttttaat gcgatggaag acttagatgt cacagagcaa
     5521 acacgcgtta ttttaatgtg tccacaaggc gagccatttt cacatcagaa agctgttgaa
     5581 ttaagcaagg ctgaccacat cgttttcata tgtggacatt atgaaggtta cgatgaacgt
     5641 atccgaacac atcttgtcac agatgaaata tcaatgggtg actatgtttt aacaggtgga
     5701 gaattgccag cgatgaccat gactgatgct attgttagac tgattccagg tgttttaggt
     5761 aatgaacagt cacatcaaga tgattcattt tctgatgggt tattagagtt tccgcaatat
     5821 acacgtccgc gtgaatttaa gggtctaaca gttccagatg ttttattgtc tggaaatcat
     5881 gccaatattg atgcatggag acatgagcaa aagttgatcc gcacatataa taaaagacct
     5941 gacttaattg aaaaatatcc attaactaat gcagataagc aaatattaga aagatataaa
     6001 ataggattga aaaaaggtta gtattgtgct aacataatct gagtgtggaa acacaaaaat
     6061 cactatgatc cgctgctata tatttgtcga ggcaagaaca taggttgaag aggagaattt
     6121 ataatgacaa atcacaaatt aatcgaagca gtaactaaat cacaattacg tacagactta
     6181 cctagtttcc gtcctggtga tactttacgt gtacacgtac gtatcattga gggtactcgt
     6241 gagcgtatcc aagtattcga aggcgttgta attaaacgtc gtggcggtgg cgtttctgaa
     6301 acgtttacag ttcgtaaaat ttcatcaggt gttggcgtgg aacgtacatt cccattacac
     6361 acaccaaaaa ttgaaaaaat cgaagttaaa cgtcgtggta aagtacgtcg tgctaaatta
     6421 tattacttac gtagtttacg tggtaaagct gctagaatcc aagaaattcg ttaatcagca
     6481 tttaaacaaa gctatgtatg agtcaaattc gactcaaaca ataaagccat ctaggtcact
     6541 tttatggagt gatatagatg gctttttttg ctgtttagtt aatgtaaagt tgataatgta
     6601 attctttatt ccttctctat tcataacgtg tttgctcaaa atggttatat aaaaaaatct
     6661 actaagtgcc attgaaaagg acttagtaga tttattaaat atatacgtag caacattatt
     6721 tttgtcttgc ccatctcgtg aaaataatac tacatataat gccaaatata gtaattgtta
     6781 taagtaaata atagtatggt ggggtatagc tcaattgaat ctttgttata tttttaggtg
     6841 ctttaatgcc ggtcataata ccatttactt gttcaacttt aagactttga tcacctgatg
     6901 tcgctttcat accttgatta tatgctgttg gcaaaacaat atacccagat gaatttttat
     6961 ttttagtaat agtataacct tgctttgtct tactaacttt gacagcctct aatgaatttg
     7021 aagcgtcttt aagcgtggta taatcttcgc cgtatatccc ttttaaattt actcgatact
     7081 tacctttagg caatgataat ctaatcctat ctgaagattt aatacgtatc gttacgggtg
     7141 ttacaaaacg acggtattta taagtgagtt tgtttctttc ttgtgtatat tcattcactt
     7201 taacatcatg cgctttatct ggcgaaagta attctaaatc catttccaaa tacaaatctt
     7261 tatattgatt tgatactgat tttggcaact gtacagttag accaccatta ttttgtttaa
     7321 cttgtaataa atgttttgta ggagattgcc aggctgcact atttaattta attgttgaat
     7381 ctgatagtaa atttttattg gctttaaaat gtgtattaac atctttagta ttgttagaaa
     7441 caatcccttg caacattgct tgttctttat ctaatggaga ttttaattct ttattggaaa
     7501 agaccttatt tgtaatatgt gcacttggat aatggatggt atttttagaa tgaatccaac
     7561 gaactttatt gtctttgtgt tcagagttaa ttttaaatcc atatggtaag ttatcatcat
     7621 gattcactct aattcgatca ttaacattcc aaagtgatag taaattttga cgattgccaa
     7681 gtagtctata agtgctgttt ttatcaattg gcatattaat ttggagtgtc ttgtcataat
     7741 attttaaaat gtctccatta aaaatactag aatataatga aatgccatta taatgatata
     7801 taaatggtga atttaatgca tagtctgaca tataatcaat gcgattaaat gggcctgttg
     7861 cattttgatt tatctttttt ataagctggt ttacatagtt actatgataa tcatgttgtt
     7921 tcaacgttga tattgattgt tgataaggtt tgattgccat atttttgttg ttatctaaaa
     7981 tgacgatttg ttgaatcata acgattaata ctaatattgc aacggttaat tttttataac
     8041 gccataaact aaattttaaa ataacggcaa gcaccattag caggataata cctactataa
     8101 gtgcaagtgg gtgtgtcggt gatagtaata cataaagtaa tgcgatgatg catacgggta
     8161 ttgttctgat taaataatat ttcatattta atgttgataa atgttgaata aacaatccgc
     8221 aaagagcact tgatgatagt gctaagatat acacccaacg cctttctgga aatgaaaaac
     8281 cattaaaagc actgtcgaaa tactgtgata atgaacctat aaataatatc catgttacta
     8341 ttgcgaaaag tctataaaag taaaaacgat acagtttgaa tgacaataat gcaacgatag
     8401 taagaattga aatcgtaata taaaatccat cgctaaagaa aaaataatga taatcaagtg
     8461 gtgtcaaaaa cggtatatca acattgggat tttgctttct gtcattttcc aaaaacgcag
     8521 aaatgccagt gaataaacca aatacacttg ataacacact caaaactgta gcagatatga
     8581 cgcaaataaa tttttgtgtt ctagagacaa tgtcatattt gtaagtgaaa atgagtcgat
     8641 ataaatagta gcaacctata ataatagctt gataataact gaaataaaaa ttgctaaata
     8701 gtgttaaggc tatcgcaaca atgaaaatac cgattttgcg ttgttgaaaa tatctttcca
     8761 aaccaagaat cgataatggc aataaatata ataaatttcc ataaaatgac caagtaaaat
     8821 taaagtatat aacgacagtt gacatgccgt ataaaatcgt agcgatcata tttgttgagc
     8881 gtttaaagtg taatatttta aataagtaga aggtcacgac aaatgttatg atagctcgta
     8941 tcatagccat aataagttgg tttgtcggcc aaaaatgtat tgttgtcgga ttaaatatac
     9001 caaccgtttc tcctatttta atgaatagaa aatttagcca cattaaaggt gacagcgaat
     9061 aataatatga tagtcctttc atataatcgc cacctaatcc aaacgatgca tcatataaac
     9121 tagaaaaact acttagatgt tcatacaaat acatttgaaa tggcatcatt tgacggaatc
     9181 catctccagc cccactaaaa acagtaccat tcacaatata atcatagata tgagtagaaa
     9241 ataaaataag cgttaatatt acactaatga aagttataac aaagaattgt ttgacgtttg
     9301 aatttagcca cttttttaac acaacattat cctcaacttt caaatttaaa attaagttta
     9361 actgaaacta aagttaatga ggttcttgat aggtaaagac gaagatgact gtggaacaga
     9421 taccttatca tagttactta aactttggat cattttcagt ttatcattaa acaaatatat
     9481 tgaataataa aaatgtcata ctgataaaga tgaatgtcac ttaataagta acttagattt
     9541 aacaaatgat gatttttaat tgtagaaaac ttgaaataat cacttttacc taaatctaaa
     9601 gcattgttaa gaagtgtgac aatgttaaaa taaatatagt tgaattaatg aatttgttct
     9661 ataattaaca ggttatagat tttaataatg agaaaagaat tgacgaaagt aaggtgaatt
     9721 gaatggttat tcaatggtat ccaggacata tggcgaaagc caaaagagaa gtaagtgaac
     9781 aattaaaaaa agtagatgta gtgtttgaac tagtagatgc aagaattcca tatagttcaa
     9841 gaaaccctat gatagatgaa gttattaacc aaaaaccacg tgttgttata ttaaataaaa
     9901 aagatatgtc taatttaaat gagatgtcaa aatgggaaca attttttatt gataaaggat
     9961 actatcctgt atcagtggat gctaagcacg gtaaaaattt aaagaaagtg gaagctgcag
    10021 caattaaggc gactgctgaa aaatttgaac gcgaaaaagc gaaaggactt aaacctagag
    10081 cgataagagc aatgatcgtt ggaattccaa atgttggtaa atccacatta ataaataaac
    10141 tggcaaagcg tagtattgcg cagactggta ataaaccagg tgtgaccaaa caacaacaat
    10201 ggattaaagt tggtaatgca ttacaactat tagacacacc agggatactt tggcctaaat
    10261 ttgaagatga agaagtcggt aagaagttga gtttaactgg tgcgataaaa gatagtattg
    10321 tgcacttaga tgaagttgcc atctatggat taaacttttt aattcaaaat gatttagcgc
    10381 gattaaagtc acattataat attgaagttc ctgaagatgc agaaatcata gcgtggtttg
    10441 atgcgatagg gaaaaaacgt ggcttaattc gacgtggtaa tgaaattgat tacgaagcag
    10501 tcattgaact gattatttat gatattcgaa atgctaaaat aggaaattat tgttttgata
    10561 tttttaaaga tatgactgag gaattagcaa atgacgctaa caattaaaga agttacgcag
    10621 ttgattaatg cggttaatac aatagaagaa ttagaaaatc atgaatgctt tttagatgag
    10681 cgaaaaggtg ttcaaaatgc catagctagg cgcagaaaag cgttagaaaa agaacaagct
    10741 ttaaaagaaa agtatgttga aatgacttac tttgaaaatg aaatattaaa agagcatcct
    10801 aatgctatta tttgtgggat tgatgaagtt ggaagaggac ctttagcagg tccagtcgtt
    10861 gcatgcgcaa caattttaaa ttcaaatcac aattatttgg gccttgatga ctcgaaaaaa
    10921 gtacctgtta cgaaacgtct agaattaaat gaagcactaa aaaatgaagt tactgctttt
    10981 gcatatggga tcgcgacagc tgaagaaata gatgaattta atatttataa agctactcaa
    11041 atcgccatgc agcgagctat tgatggatta tcagtacaac caacgcattt attgatagac
    11101 gcgatgacgc ttgataatgc actgcctcaa gtatctttaa tcaagggtga tgcaagaagt
    11161 gtgtccattg cagctgcaag tatcatggca aaggtttttc gtgatgatta tatgacacag
    11221 ttatctaaaa attatcctga atacggtttt gaaaaaaacg cgggttatgg taccaaacaa
    11281 catttactag caatcgatga tattggcatt atgaaagagc atagaaaaag ctttgaacct
    11341 ataaaatcgt tactgtaaat cgtaaaattc aattaaatga taaaaaagta gcatttaagt
    11401 ttacaatagc gcttacattt tttataattg taagggaact taacctaagt aacaggagga
    11461 tggaagatga atatccacga gtatcaaggt aaagaaatat ttcgttcaat gggcgttgca
    11521 gttccagaag gacgagtagc atttactgct gaagaagcgg tggagaaagc aaaagaatta
    11581 aattctgatg tttatgttgt aaaagcacaa attcatgctg gaggtagagg taaagcaggc
    11641 ggagtaaaaa ttgctaaatc tttatcagag gtagaaacat atgcaaaaga attattaggg
    11701 aaaactttgg tgacacatca aactggtcca gaaggtaaag aaattaagcg tttatatatc
    11761 gaagaaggtt gtgctattca aaaagaatat tacgttggat tcgttattga tcgtgcgact
    11821 gaccaagtaa cattgatggc gtctgaagaa gggggcactg agattgaaga agttgctgcg
    11881 aagactcctg aaaagatctt caaagaaact atcgatccag taatcggact ttcaccattc
    11941 caagcaagac gaattgcgtt taatattaat attcctaaag aatctgttaa caaagctgct
    12001 aaattcttat tagcacttta taatgtattc attgaaaaag attgttcaat cgtagaaatc
    12061 aacccattag ttacaacagc tgatggtgat gtattggcat tagatgctaa aattaatttt
    12121 gatgataatg cattattcag acataaagat gttgtagaat tacgtgattt agaagaagaa
    12181 gatccgaaag agattgaagc gtctaaacat gatttatcat acattgcatt agatggtgac
    12241 atcggatgta tggttaatgg tgcaggttta gccatggcaa caatggatac gattaatcat
    12301 ttcggtggaa acccagccaa tttcttagat gcaggcggaa gcgctactag agaaaaagta
    12361 actgaagcat ttaaaatcat tttaggtgat gaaaatgtta aaggtatttt tgtaaacatt
    12421 ttcggtggca ttatgaaatg tgatgttatc gcagaaggta tcgttgaagc tgtaaaagaa
    12481 gtagatttaa ctttaccact agttgtacgc ttagaaggta caaatgttga gttaggtaaa
    12541 aaaatcttaa aagactcagg attagcaatt gaaccagcag caacaatggc tgaaggtgca
    12601 caaaaaattg ttaaactagt caaagaagca taaggaaagg atgggagcac taagatgagt
    12661 gtatttatag ataagaatac taaagtaatg gtacaaggta ttacagggtc tactgccctt
    12721 ttccatacaa aacaaatgct tgattatggc actaaaatag tagcaggtgt gacgcctggt
    12781 aaaggtggtc aagttgttga aagcgttcct gttttcaaca ctgttgaaga agctaaaaat
    12841 gaaactgggg caacggtttc agtcatttac gttccagcac catttgctgc agactcaatt
    12901 ttagaagcag ctgatgcaga cttagatatg gttatttgta tcactgaaca tattcctgta
    12961 ttagacatgg ttaaagttaa acgctactta caaggtagaa aaacacgttt agttggtcca
    13021 aactgtccag gtgtgattac agcagatgaa tgtaaaattg gtattatgcc tggctatatt
    13081 cacaaaaaag gtcatgttgg tgtagtatca cgttcaggta cactaacata tgaagcagtg
    13141 caccaattga ctgaagaagg tattggtcaa actacagctg ttggtattgg tggagaccca
    13201 gtcaacggaa caaactttat tgatgtttta aaagcattca atgaagatga cgaaacgaaa
    13261 gcagttgtta tgattggtga aatcggtggt acagctgaag aagaagcagc tgaatggatt
    13321 aaagcgaata tgacaaaacc agttgtaggc tttatcggtg gacaaacagc tcctcctgga
    13381 aaacgtatgg gacatgctgg tgcaatcatt tcaggtggta aaggtactgc tgaagagaaa
    13441 attaaaacat taaatagttg tggtgtgaaa acagcggcaa caccttcaga aattggttca
    13501 acattaattg aagctgctaa agaagcaggt atttatgaat cattattaac agttaataaa
    13561 taaagttaaa agatgatatt aatggctata gcccattcca tactttataa aagtatcgga
    13621 aatgggctat ttgtcgtgcg atgaatttta gtacactttt catatttcgg aaaggcaagt
    13681 taattatttt acctccttca atagctttat aaaacaaagc aaatttttat taaaatatat
    13741 agcgcactat gttataatat ttatatatta ttgcaaggaa tgtttcatca tgaataaaca
    13801 acaaagtaaa gtacgctatt caattagaaa agttagtatt ggaacatttt taaatggtga
    13861 ttatgatgga aaattaatga agttccaaag tttagatcaa aactggaata atggtggatg
    13921 gcgtaaagca gaggttgcac ataaagttgt tcataattat gaaaatgata tgatttttat
    13981 tagaccactt aaaaaagcat aatttaaatc gaaggcagga cattgaaata tgaaattttc
    14041 aactttaagt gaagaagaac ttaccaacta caccaaaaag cacttcaaac attatacaca
    14101 gtctatagaa ttatataatt atagaaataa aataaatcat gaagcacata ttgtgggagt
    14161 gaagaatgat aaaaatgaag ttatagctgc atgtttatta acagaggcac gtatttttaa
    14221 attctacaaa tatttctact ctcatagagg tcctttactt gattatttcg atgctaaatt
    14281 agtttgttac ttttttaaag aattatctaa attcatttat aaaaatagag gagtatttat
    14341 tcttgttgat ccatatttaa tagagaattt aagagatgca aatggtagga taataaagaa
    14401 ttataataat tcagtgatag taaagatgct aggaaaaatt gggtatctcc atcaaggtta
    14461 tacaacagga tattcaaata aaagtcaaat taggtggatt tctgtattgg attaaaagat
    14521 aaagatgaga atcaactttt aaaagaaatg gaataccaaa ctagaagaaa tataaaaaag
    14581 actattgaga ttggtgttaa ggttgaagat ttatctattg aagaaacaaa tcgattttat
    14641 aaattgtttc aaatggctga agaaaaacat ggttttcatt tcatgaatga agattatttt
    14701 aaacgaatgc aagaaatata taaagataag gcaaagttaa agatagcttg tatagatctt
    14761 aatgaatatc aagataaatt aaaaatacaa ttattgaaaa tcgaaaatga aatgatgact
    14821 gtgaacagag cattaaatga aaatccaaat tctaaaaaga ataaatcaaa attaaatcag
    14881 ttaaatatgc aattatctag tattaataat agaattagta aaaccgaaga actaatatta
    14941 gaagatgggc ctgttttgga tttagctgct gctttattta tatgtactga tgatgaagtt
    15001 tattatctat caagtggatc aaatcctaaa tataattagt atatgggcgc atatcatcta
    15061 caatggcata tgataaaata tgcaaaatca cataatatta ataggtataa tttttatgga
    15121 ataacaggcg tctttagtaa tgaggcggat gattttggtg tacaacaatt taaaaaaggt
    15181 tttaatgcac atgttgaaga attaattggt gatttcatca aaccagtaag accaattcta
    15241 tataaatttg caaaacttat ttataaggtt tgattataaa gtatgttgga aattgaaatt
    15301 taaaattctt tccaacatac ttttcacttt ttaagtcggt attttactcc ttaaaataac
    15361 attacatcga gaatgttttt gtattagaaa tgtaaaacaa aaatagaata tgattttgta
    15421 tgattaaaaa aggagttgat ttattgatta gactattttt gcttaagtta tactgggcac
    15481 acttttcgac taaacaaatt catcaatttt taatggcata tcctaatgta attaaagagg
    15541 ggggcagaaa aaaagatagt tatttatgtg aatgggtgaa
//
LOCUS       DAPIDM010000053        15460 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000053 DAPIDM010000000
VERSION     DAPIDM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.053"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            202..519
                     /locus_tag="RJE42_001434"
     CDS             202..519
                     /locus_tag="RJE42_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDV5876858.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            763..1905
                     /gene="bshA"
                     /locus_tag="RJE42_001435"
     CDS             763..1905
                     /gene="bshA"
                     /locus_tag="RJE42_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDV5876859.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVRDKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            1910..3112
                     /locus_tag="RJE42_001436"
     CDS             1910..3112
                     /locus_tag="RJE42_001436"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDV5876860.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMI
                     RCLRFQSQLSFEIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHHLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVNNHVKI"
     gene            3099..4070
                     /locus_tag="RJE42_001437"
     CDS             3099..4070
                     /locus_tag="RJE42_001437"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDV5876861.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     DFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            4094..6787
                     /locus_tag="RJE42_001438"
     CDS             4094..6787
                     /locus_tag="RJE42_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV5876862.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNHIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAEEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKNCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            7109..8401
                     /gene="asnS"
                     /locus_tag="RJE42_001439"
     CDS             7109..8401
                     /gene="asnS"
                     /locus_tag="RJE42_001439"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV5876863.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            8730..9416
                     /locus_tag="RJE42_001440"
     CDS             8730..9416
                     /locus_tag="RJE42_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDV5876864.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            9406..10065
                     /gene="nth"
                     /locus_tag="RJE42_001441"
     CDS             9406..10065
                     /gene="nth"
                     /locus_tag="RJE42_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV5876865.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            10070..10411
                     /locus_tag="RJE42_001442"
     CDS             10070..10411
                     /locus_tag="RJE42_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876866.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(10929..13112)
                     /locus_tag="RJE42_001443"
     CDS             complement(10929..13112)
                     /locus_tag="RJE42_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDV5876867.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEEAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDVKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGEIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSAHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(13109..13786)
                     /gene="recU"
                     /locus_tag="RJE42_001444"
     CDS             complement(13109..13786)
                     /gene="recU"
                     /locus_tag="RJE42_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDV5876868.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEIFWKRYQDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            14274..14624
                     /locus_tag="RJE42_001445"
     CDS             14274..14624
                     /locus_tag="RJE42_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDV5876869.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     LLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            14617..15180
                     /locus_tag="RJE42_001446"
     CDS             14617..15180
                     /locus_tag="RJE42_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDV5876870.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            15194..>15460
                     /locus_tag="RJE42_001447"
     CDS             15194..>15460
                     /locus_tag="RJE42_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDV5876871.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNT"
BASE COUNT         5626 a         2141 c         2694 g         4999 t
ORIGIN      
        1 aatcataaag ttataatctc aatttcaatt gatttaattt agttaggaat gcattacatt
       61 tatataaact gaatgattaa acttctaaaa taaacaactt gtcatctagt ttagctagag
      121 tcagtagatt gcactaaaca tgcatgagac ttcaaattga tatatactat attagaaatg
      181 caatataagg agaacgtaga aatgaaatca atggtagaaa tgcaacgtga agttgatgaa
      241 tatattggac aatttaaaac aggatacttt tcaccattag ctaacttagc tagattgact
      301 gaagaagtgg gcgaacttgc acgtgaaata aatcatacct atggtgaaaa aaagaagaaa
      361 gattcagagg aagcaaatac gattaaagca gaattaggtg ataatttatt tgtgttgtta
      421 tgtttagcga attcaatggg aatagatatg acagagagct ttaatgaaac aatggaaaag
      481 tttaatacaa gagataaaaa tcgattcgaa agaaagtgaa tataaaaata tatgctttaa
      541 ttggatttga ttaagattaa aaacatccat caatgaattg catcgcatga aatcatatta
      601 tttttaaagt aagtataact agataaatga ttatatatac aacacctgtt ttttagatat
      661 ttgaaattat aaactcaaca ttactataaa cttaaattta aacaattatc tacgcatcga
      721 gaataataga aagagaaata gaaagaaagg atgggcaagc gaatgaagat aggtataaca
      781 tgttacccgt ccatgggtgg ttctggaatt attgccacag aattagggat taaattagca
      841 gagcgaggtc acgaagtaca ttttatcact tcaaatatcc cgtttagaat aagaaaacca
      901 ttaccgaata tgatttttca tcaagttgaa gtgaatcaat atgcagtttt tcagtatcca
      961 ccgtacgata ttacgttaag tactaaaatc gctgaagtta ttaaagaata tgatcttgat
     1021 ttgttgcata tgcactatgc tgtacctcat gcaatttgtg ggattttagc tcgtgagatg
     1081 tcaggtaaag atataaaaat tatgacaacg ttacacggta ctgatattac tgttttgggc
     1141 tatgatcatt cactccaagg tgcaattaaa tttggcattg aaaaaagtga tattgtgaca
     1201 agtgtgagta aatctttagc gcaagaaaca catgaaatta tcgaaactaa caaagagatt
     1261 ataccgattt ataattttgt acgtgaaaat gagtttccaa ctaaacataa cacagcgtta
     1321 aaatcacaat ttggtatagc accagatgaa aaagtgttaa tacatgtatc gaattttaga
     1381 caagtaaaac gcatagatac tattatagaa acatttgcaa aagtacgtga taagatacct
     1441 agtaaattaa ttttacttgg agatggtcct gaattagtgc caatgcgtca attgacgaaa
     1501 gagttaaacg tagaagagga tgttttattt ttagggaaac aagattgcgt aagtgaattt
     1561 taccaattat ctgacttagt attattatta agtgaaaaag aaagctttgg actaacttta
     1621 cttgaggcta tgaaaacagg cgtcgttcca attggttcaa atgccggtgg aattaaagag
     1681 gttattaaac atggtgaaac tggatttgtc gtagatgtgg gtgattgtga ttcggcgagt
     1741 gactatgcta ttcgattact tgaagataag gtgttataca acaaacttca gaaaaacatg
     1801 ctagcagata ttgctgaacg ttttggatca gaactcatta cagatcaata tgagtattac
     1861 tatcaaaaaa tgctaaatga gcataacaaa agtaaaggcg agtaataata tggataaatc
     1921 attatttgaa caggcaaggc ctatattaga acaaattcaa gacaatggtt ttgaagcata
     1981 ttatgtaggt ggctctgtaa gagattatgt catgggaaga aatattcatg atatagatat
     2041 cacaacaagt gcaacgccag atgaaataga atctatcttt agtcatacga tacctgtagg
     2101 taaagaacat ggcaccataa atgtagtttt taatgatgaa aattatgagg tgacaacatt
     2161 ccgggctgaa gaagattatg tcgatcaccg tagaccaagt ggtgttacat ttgttcgtga
     2221 tttatacgaa gatttgcaac gaagagattt cacgatgaat gcgatagcaa tggatacagc
     2281 atacaaattg tatgattatt ttgatggtca acaagatatt aataatcgaa taataagaac
     2341 tgtaggtata gctgcggaac gattccaaga agatgcttta cgcatgattc gatgtttaag
     2401 attccagtca caattatcat ttgaaattgc aacggaaaca tttgaagcga tgcgtacaca
     2461 aatggcagat attaaatttt tatcaattga gcgtatagtg attgaactaa ctaaattaat
     2521 gcgaggtatt aatgttgaag agagttttaa tcatttaaaa tcgctgaaag catttaatta
     2581 tatgccatat ttcgaacatc ttgatatgaa tcaaattaat gtaactgaac caattgattt
     2641 agaattgttg attgctatag tgtcagttaa atttgatatt aattactcat tgaagccttt
     2701 aaagctaagt aaccgacaag taaaagatat caatcaatat attcaaatta tgaatgcatt
     2761 accaagtatt attacaaaag aacaattaaa aatgtttgtt tatgattatg atacgaatct
     2821 cattaaaaat gtaatggttg cagcagacgt gttaaaggca aatgatattc aaggacatga
     2881 accattaatc gttaaccttc aaacgattga tgaaacatta catcatttac ctatgcataa
     2941 tagaaaagac atgatggtta atggcggtgt acttatggca catttaaatg ccaaaagtgg
     3001 tccgtggtta aaagatgtgc taagacaaat tgagatagcg attgtaacag gtaaagtaag
     3061 caacgaagaa actgaaattt tgaaatgggt gaataatcat gtcaaaatat agtcaagatg
     3121 tacttcaatt actctataaa aataaaccga attatatatc tggacaaagc attgcggaat
     3181 cacttaatat atcgcgcact gcagtaaaaa aagtgattga ccaattaaag ttagagggat
     3241 gtaaaataga ttcagtaaat cataaaggac atttattaca acaactccca gatatttggt
     3301 atcaaggtat aatagaccaa tacacaaaaa gttctgcttt gtttgatttt agtgaagtat
     3361 acgattcaat agattctaca caacttgctg cgaaaaagtc acttgttgga aatcaatctt
     3421 cattttttat cttgagtgat gaacaaacga aaggtcgtgg gcgatttaat agacattgga
     3481 gttcttcaaa agggcaagga ctttggatgt ctgtcgtgtt aagacctaac gttgcattct
     3541 caatgatatc taaatttaat ttatttattg cattagggat aagagatgcg attcaacatt
     3601 ttagtcaaga tgaagtcaaa gtaaaatggc cgaatgatat atatattgat aatggtaaag
     3661 tgtgtggttt cttaactgaa atggttgcta ataatgatgg tatagaagca ataatatgtg
     3721 gtataggtat taatttgacg caacaactag aagactttga tgaaagtatt agacatagag
     3781 caacaagtat acaattacat gataaaaata aattagatag atatcaattt ttagagagat
     3841 tacttcaaga aattgaaaaa agatataatc aatttttaac gttacctttt tctgaaattc
     3901 gtgaagaata tattgcagct tctaatattt ggaatagaac gttgctattt acagaaaatg
     3961 ataaacagtt taaaggacaa gcaattgatt tagattacga tggctatcta attgttagag
     4021 atgaagcggg tgaatcacac cgtttaatta gtgcagatat agatttttaa cactgaagca
     4081 aggagagata gctatgggta tggcaaccta tgccgttgtg gatttggaaa caacaggcaa
     4141 ccaattagat tttgacgata tcattcaaat tggtataacg tttgtgcgca ataaccatat
     4201 tattgataca tatcattcta tgattagaac aaatttagag attccgccat tcattcaagc
     4261 gttaacatcc attgaagaaa atatgctaca acaagcacca tattttaatc aagttgcaga
     4321 agaaatctac gataagataa aagattgcat ttttgtcgct cataatgtag attttgattt
     4381 aaattttatt aaaaaggcat ttaaaaattg taatattcaa tatcgtccca aaaaagttat
     4441 agatacacta gaaatcttta aaatagcttt tcctacagat aaaagttatc aattaagtga
     4501 attagcggag gcacatggta ttaccttagc aaatgcccat cgagctgacg aagatgccgc
     4561 tactactgct aaattgatga tattagcttt tgaaaaattt gaaaagttac cgttagatac
     4621 tttaaaacaa ttatattatt taagtaaaca gttgaaatac gatttgtatg atattttctt
     4681 tgagatggtt aggcaatatg atgcaaagcc attggataaa tcgtacgaaa agttcgaaca
     4741 gataatatat cgtaagcaag ttgattttaa aaagccgaca acaaattata atggcagctt
     4801 gaagtcttta tatagcaaag ctgtagatca acttggctta acatatcgac cgcaacaatt
     4861 atatttagcg gaaactatat tagatcagct catgcatagt gaaaaagcaa tgattgaagc
     4921 atcactaggc agtggtaaat cattagcata tttacttgcg gccttgatgt ataatattga
     4981 aactgggaag catgttatga tttctaccaa taccaaatta ctgcaaagtc aattattaga
     5041 aaaagatatt ccagcaatga atgaagcatt gaattttaaa attaatgcac tattgattaa
     5101 aagtaaaagt gattacattt cattgggact tattagtcaa attttaaaag atgacacaag
     5161 taattacgaa gtgaatatct tgaaaatgca attgctgatt tggattacag agacgccatc
     5221 cggagatata caggaattaa atttaaaagg tggtcaaaag atgtattttg accaaaaaat
     5281 tgaaacatat gtaccggcac gtcacgatgt tcactattat aattttatta agcgaaatgc
     5341 acagaacatt caaattggta ttacaaatca tgcacattta attcattctg atgttgaaaa
     5401 ttcaatatat caattgtttg atgattgtat agtggatgaa gcacatcgct taccagacta
     5461 tgccttaaat caagtgacaa atgagttgag ttatgctgat attaaatatc agcttggttt
     5521 gattggtaaa aacgaaaatg aaaaattgtt aaaggcaatt gatcaactgg aaaagcagcg
     5581 aattttggaa aaattagata ttgctccaat tgatattttt ggcttgaagg ctagtatgaa
     5641 tgagattcat gagttaaatg agcaattgtt ctcaacgata tttacgatta tcaatgattc
     5701 tgatgtttat gatgatgata ttcatcgctt ccataatgta tttacttttg aaacaaaaga
     5761 cattttgaag gatttacatg cgattatcga taagttaaat aaaacgctag aaatattcaa
     5821 tggcattagt cataaaacag ttaaatcatt acggaaacag ttgttgtatt taaaagataa
     5881 atttaaaaat atcgaacaaa gtttaaaagc tggacatact agtttcattt caattaaaaa
     5941 tttatctcaa aaatcaacaa tacgtttata tgtgaaagat tatgctgtaa aagatgtatt
     6001 aacgaaacaa gtattggaaa aatttaaatc actcattttt atatctggta ctttaaaatt
     6061 taatcattcg tttgaggctt ttaaacagtt attcaacaaa gatgttcact ttaatacatt
     6121 tgaagttaac acgtcgttac aaagtgcaaa aaatacaagt gtatttatac cgagtgatgt
     6181 agcatcttat caatataaaa atatagatga gtatgtagca tcgattgtaa gctatattat
     6241 tgaatatacg actataacgt catcaaaatg tttagtctta ttcacgagtt ataaaatgat
     6301 gcatatggta caagatatgc taaatgaatt gccagaattt gaagattatg ttgtattaac
     6361 acaacagcag aaccaaaatt ataaaatagt acaacagttt aataattttg ataaggccat
     6421 actgcttggt acgtcaacat tttttgaagg ctttgatttt caagcaaatg gtattaaatg
     6481 tgtgatgata gcgaagttac cgtttatgaa caagcataat gcaaaatatt ggcttatgga
     6541 ttctgaattc acttcaactt tcaaggaata tgtattacct gatgcagtta cacgttttag
     6601 acaaggactt ggaagattaa ttagaaatga aaatgatcgc ggcataatag tttcatttga
     6661 tgatcgactc ataaatagta attataaaaa cttttttgaa caaacacttg aaaattatcg
     6721 tcagaaaaaa ggcgacatac aacagtttgg aaaattatta agacaaattc aaaaaaagaa
     6781 aaagtaaaat acaaagtaaa attcttgtga catgacatgt gctatgttct gttacaagaa
     6841 tttttgtttg gtcgtcaaaa tttgagtttt ataattaaac ttgaatgtta gcacaaaaaa
     6901 cgaccttcat actaataata ttgaggcttt attatatatt ttgtctgaaa ctgatgattc
     6961 tattgtctta atataaataa atgttcaaat ctgtgcttaa atcattacca tttacacgtt
     7021 atcttttagt ctagttctag aacttgaaag cggattttgt taaaatgaat gagtattgta
     7081 taacgaaaaa ataaatagga gaatggttat gaaaacaacg attaaacaag cgaaagatca
     7141 tttaaaccaa gacgttacaa ttggtgcttg gctaacaaat aaacgttcaa gtggtaaaat
     7201 cgccttttta caattacgtg atggaacagg ctttatgcaa ggcgtagtag ttaaatcaga
     7261 agttgatgaa gaggtattca aacttgcgaa agaaattact caagaatcat ctctatatgt
     7321 tacaggcaca attacagaag ataatcgttc tgacttagga tacgaaatgc aagtaaaatc
     7381 aattgaagtt atttcagaag cgcatgacta tccgattaca cctaaaaatc acggtacaga
     7441 atttttaatg gatcaccgtc atttatggtt acgttctaaa aaacaacatg ctgtaatgaa
     7501 aattagaaat gaagttattc gtgcaacgta tgaatttttc aacaaagatg gatttacaaa
     7561 ggttgatcca ccaattttga cagcaagtgc accagaaggt acaagtgaat tattccatac
     7621 taaatacttt gatcaagatg cgtttttatc tcaaagtggt cagttatact tagaagctgc
     7681 agctatggca cacggaaaag tattttcatt tggtccaact tttagagctg aaaaatcaaa
     7741 aacacgtaga cacttgatcg agttctggat gattgaaggg gaaatggctt tcacaaatca
     7801 tgctgaaagt ttagaaattc aagaacaata tgtaacacat gtagtaaaat cagttttaga
     7861 aaattgtaaa ctagagttga aaattttaga gcgagataca tcaaaacttg aaaaagttgc
     7921 gacaccattc cctagaattt catatgatga tgcaattgaa ttcttaaaag cagaaggctt
     7981 tgatgatatt gaatggggcg aagattttgg tgcgccacat gaaactgcca ttgctaatca
     8041 ttatgattta ccggtgttta ttactaatta tccaactaaa attaagcctt tctatatgca
     8101 accaaatcct gagaatgaag aaactgtatt atgtgcagac ttaattgcac ctgaaggata
     8161 cggtgaaatt ataggtggat ctgaacgtgt ggatgactta gaattgttag aacaacgcgt
     8221 taaagaacat ggattagacg aagaagcata tagttactac ttagacttac gtcgttatgg
     8281 tagtgtacca cactgtggat ttggtttagg tttagagcgt acagtagcat ggatttctgg
     8341 tgttgaacac gttcgtgaaa ctgcaccatt cccaagatta ttaaaccgtt tatatccata
     8401 agttttagga tcgttactta aaatttatat tatgataata aaaagaatcg tccagccttt
     8461 attaatagct gggcgattta tgcttggaaa atgtaaattg ataacgggtt tcattaagca
     8521 tgaacaaact tttgtttagt aactatattt ggaaaataaa tagctcatct gattatacat
     8581 aaagttcatt gttaaaaact agttttaaaa aaatactgtt atttaaaatt tgtttgctac
     8641 tagttaaata tattaagcat tttagtcgaa aattaaagaa aatgatttat actattaagt
     8701 aatggttaaa tttaaaaggg ggcacgaaca tggataaata tcaattaaaa gcaagacctg
     8761 tagtgatacg aagagaatta ttagaccatt acagtgactt aggtttagat gagcaagatt
     8821 tagtcatttt gcttaaattg atttatgctt ccgaaacatc aaataaacag ccttcaattg
     8881 aattacttca gaagggctca actatgcaac cacgcgatat tacaatggtt attcaaaatt
     8941 tgatacaacg tgaattgcta gaattacaag ttcaaaaaga tgaagaaggt agatttactg
     9001 aatatatgaa tctagatcct tttttcgaaa aattaagtca tatattgaag caacaaagta
     9061 tggaaacgaa agagcaaaat agtaaagaaa aatttaaaca attatttaga gttttagagg
     9121 atacatttgc aaggccgtta tctccatatg aaatagaaac gttaaatcaa tggattgatg
     9181 ttgataaaca cgatactgca attatacaag cggcattaga cgaggcgaat agtttgaata
     9241 aacttagctt taaatatatg gatcgtattt tacttaattg gaagaaaaat aatgtcaaaa
     9301 caattgatga ttctagaaaa ataagagaaa aatttaataa accgaaaatg acgcacacag
     9361 ttaaaacggt acctaaattt gattggttaa acggggagaa ccttgatggt aagtaagaaa
     9421 aaagcattag aaatgattga tgttatagca aatatgtttc cggatgcaga atgtgaatta
     9481 aaacatgata atccgttcga attaactatt gctgtattat tgtcagcgca atgtacagac
     9541 gttctagtaa atagagtaac aactgagctg tttaaaaaat acaaaacgcc tgaagattat
     9601 ttggcagtca gtgatgaaga acttatgaat gatattcgtt ctatcggtct gtatcgtaac
     9661 aaagctaaga atattaagaa actttgtcaa tctttgattg atcaatttaa tggagaaata
     9721 ccacaaacac ataaggaatt agaaagttta gcaggtgtag gacgtaaaac tgctaatgta
     9781 gtcatgagtg tagcatttga tgaaccttca ttagctgttg atacgcatgt agaacgtgtt
     9841 tctaaacgct taggtattaa tcgttggaaa gataatgtga gacaggtaga agaccgttta
     9901 tgttctgtca tacctagaga tagatggaat aggagccatc atcaactcat tttctttgga
     9961 agataccact gtttagcccg aaaacctaaa tgcgatattt gtccactatt agaagattgt
    10021 agagaaggac aaaaacgtta taaagctagt ttgaaagaag cgtgataaca tgacaaatat
    10081 tacaaaagag gtatttgata atttagaaca agagattgat gtatttgcca aaaataagac
    10141 attaggttct agtgaagcaa aaccttactt ggatgaatac catagtaaaa ttattgatta
    10201 ttttaagcag gttaatgaca taactggcaa tattgatttt gataatttaa atcaatatcc
    10261 tgttgtgcca atgaatttta aagaaagata tgattatatg attgaacgta aatatcattt
    10321 tatgggctat cgacaaatga agacctttaa aactgaattg attaaaatga atgctagtta
    10381 tcaaacaagg ttaaagaata agcaggtata gacaagtttg atcatttcct aatgttgaaa
    10441 agatgtaaaa ctaattcatg cacacatatt tgtatttatg ttaggtgtcg tgtcattaca
    10501 acgtcttaat ataaaaactg tccatctaca tcataatgga tatcttgagg tatgctttaa
    10561 gatatctatt tcgaatcgta ggtggatttc ttatatctat gattgtgaaa atatgttgca
    10621 aacatgttga tagttcactg attaacgtac tcagggaatt gataaacatt tcaatttaaa
    10681 ataacgtcta agatgactat gagaaaatat aatcataatt ttaaaaatgg gaacatccat
    10741 ttttgcaatt tcaataattt tacatagcta aagatgtgaa tatgaaagac tatttagatg
    10801 tttcaaaatg tatgtgaatg tctgatttta cggtttctac taatatagca atagcgtatg
    10861 aatacttata aaaaaatcct cagctacacc ataaatatgg tataactgag gattttatgt
    10921 tgagtggatt agttgaatat acctgttaat ccaccgctgt ttctagattg ttgagatcta
    10981 gtattgttat ttgattgtgc agtaccactg ctgtttgctg atgtgtcact accaccgtgt
    11041 gcactacgat ttgtagtgtt gttatcttga ctaccagaaa cattgattga tgggatacta
    11101 ccacttactg agctaggacg tttaaagtct tcgccatctc tagatgaaat ttttgacatc
    11161 acattttcat ataagaactg tggatattct tgttggctat gtcccacaaa tgagttttca
    11221 ccatattgtt taactttgct gaagcccatc cacactgaca tagtgtattg aggtgtaaag
    11281 ccgttaatcc acacgtcttt cgctgcatta tcaggtaaat tatattgtga ataagtttca
    11341 gcaccgtaag taccagtacc tgtcttagca cccatattta ctccagatac accatggcca
    11401 tatgcagaac catatggttt aaatgtacct tttagcatct cagctaacat gtatgcagtg
    11461 taatcactca tcgctttatg gctagtatga tcgtattcga ttgtttcacc atcacgagta
    11521 actacttttt gaattgaatg cgcgttgtta taagtaccac cgttagcgat tgcagcaaat
    11581 gctgatgcta attgtgttgg tgagaattct gaagcagaac caccaagtac ttcagatgga
    11641 ccaatttcgc cttcgtagtt taagccaagt ttggcagcga atttcttagg tgcatcatta
    11701 ccagcatttt gcttaactga ttgccaagct tttaaagctg ggatattgaa actttgtcgt
    11761 aaagcatcat aaattgatac agtaccgtga ctcttcacat cgtagtttct aaatgtagat
    11821 ccatcaactt gatatgaaga ttcatcttga atcgcatggt ttgttgccca tttcatattt
    11881 tcaatggcag gtccatacgc taagaaaggt tttaaagacg aaccagtagg gtgtggatct
    11941 gttgcttggt ttctgttaac gacgtctttg aaatcacgtc caccagatat agcaactaaa
    12001 ccaccagttt tactatcaag aatcgttgca ccaacttgtt ggtctttatt cttgtagaag
    12061 ctaccattat caacatcatt ttgtaatgtt ttttgaacat ctttatccat gtttgtataa
    12121 attttaatac cactttgtaa tacattacct aaattttcat ctttgaatgc tttattattc
    12181 attaattcag attttacaaa gttaacgtat gaattatact cagaatcttg atttgtatca
    12241 atgttttgac gttcttcagc agtacgattt actaagttcg cttttaaatc gattttctta
    12301 gcttcttccc actgtttatc tgtaatgcgt ttatgataat gcattaagta taaaacagtg
    12361 tttttacgat cttcagcagc ttttggatga tcataaatgt tatagttgtt tggaacctga
    12421 ggtaaaccgg ctaaataagc ttcttccgct aagtttaaat cttttaaatc tttattaaag
    12481 taatacttag cagcagcttt aatacctgtt acgccatcag aatagtaaat tttatttaga
    12541 tatacttgga agatatcatc tttactatac tcttgttcta aacgatatga taagtatgct
    12601 tcttgagctt tacgtccaat agatttatgt tgtgataaaa atgcatcttt aacaacttgt
    12661 tgcgttaatg ttgaggcacc ttcagaacca aatccaccag tcaagttctt accaattgca
    12721 ccgaataaac gtttataatc aagtgcgcca tgttcgtaga aacgattgtc ttcagttgca
    12781 agtactgcgt ctttcattga tttcggcacg tcttttaaat ttacatgctc atgtctttgg
    12841 ccattatcta atgttttaac aagttctccg ttcttgtcat atatctttgc aggaatcgga
    12901 tcttgtaatt tagcttcggt aaaagcaggt gctttccaag cataataagc aaacaataag
    12961 atacctagta aaagaacaac gaaaaatgca ataatcatga agccaataat cttaataatc
    13021 gttctcttca catttctatt cttttttgag ttggatttcc caccattatt accatttttc
    13081 ttaggctgag aagatccttt gttttccgtc atacgcggtc ctcactttca tctaatatca
    13141 acttatcaac tgcttttaga tagtctaatc ttggttgata ctgataagga atatggtaac
    13201 cattttttcg tatttcatca actgttatag actttttaat attatcttga tatctcttcc
    13261 aaaatatttc gaattttgaa tagggtaaaa gatatacttc atctagcgtt ttaaaacgaa
    13321 tcattaaaaa cacaatacct ttttgttgat atgcattttt catatgttcg acttgatggt
    13381 catgaatatt atttaaagga aaggacgtct tgtttttagt ttcctttgct tcaaaatcaa
    13441 tataataacc ttgataaacg ccgttgtaat cagttgttga aggtgtacga aaataagctt
    13501 cgttaatcac agctttactc cgtttaggat agttgacatt aactatttgt actggcgtag
    13561 gctttttgtg aataactgca atgtcgcttt taagataaaa cgcatttgaa tgttcgatat
    13621 ctttttcaag tgacatacca cgtccaccat actcaatatt actaaaggca gcggtctttt
    13681 tccctccgtc tatagcacta tttttacgat atggtttacc atttggataa ttcataaatt
    13741 tcaccacaca agtttggtag taaacgttgt ttattataca caacattata gtgaaacacc
    13801 aaacttaaat tagtataaat gcgaaagaca caatacacca aagcttattt taacgtgatt
    13861 tcagcattaa cacaataata ttataataca tttaccgtta atgttggaat tttacacaca
    13921 attttaatta attgaaaagt tttgcttata aaaatgaata ctcattacaa tatttttgaa
    13981 ttttgttata aaagtcaaat catgatcagt aattattatt tttgaggcaa gtacaagtat
    14041 gtgttgttaa tcaacagagg tgtatttgat ttaaatgatt ttattagatt aaaagtattt
    14101 tcatcgtata acaacataca cgataaaatt ttgtatttat ataggaagtt tttagattta
    14161 cttgatttaa cagataacct atttgaaaaa aagttaaatc aaattagata gagtgttaaa
    14221 acatgacgaa tacagtatat tgtgataaat taaaaaatgt aggaggcgtc ctcatgaatg
    14281 atgtagtcga atcactaatt tatgaagtta acaacatgca acaaaatttc gaaaatgtga
    14341 aatcacaaca acaagatcat gatttttatc aaactgtaaa gccatatact gaacatattg
    14401 atagcttgct caatgaaatc aaattacatc gtgaatttat aatagaagta ccttatatga
    14461 attcaaggaa atttgagcta ctgattgcta acattgaaca actttctgtc gaatgtcatt
    14521 ttaagcgaac aagtcgaaag ttatttattg aaaagcttaa aagtgttcaa tatgatttac
    14581 aaaatatatt agatggcgta actaaagagg gtacttatgg ttaaaacagt ttatgtaaca
    14641 ggttacaaat cattcgaatt aaacattttt aaagatgacg cacctgaagt acattattta
    14701 aaacaattta taaaacataa aattgaacaa ctgttggatg aaggattaga atgggtgtta
    14761 atacaagggc aaatgggtat agaattatgg actgcagaag ttgttattga attacaacga
    14821 acatatgatt cgttaaagtt tgctgtaatt acaccgttcc aaggacatac agaaaagtgg
    14881 aatgaacata atcaaagtaa gtatgccaac ataattaagc atgcagatta tgttgatagt
    14941 atttttcata cgtcgtatca aggtcctttt caatttaaac aagcagatca atttatgctt
    15001 gaacattcgg atcaaacatt gctcatttat gatgaggaac aagaggcgag tcctaagttc
    15061 ttcaagcaga tgttagttga ttttatggat aaaacaaact atacttgtga tattgtgacg
    15121 ttcgatgaat taacagcatt catcaatgac ttacagtggt ctgaagatca aagtttctaa
    15181 tgaggtggaa aaaatgtcag atgtttcatt gaaattatca gcaaaagata tttatgaaaa
    15241 agattttgaa aaaacaatgg ctcgtggcta tagaagagaa gaagtagatg catttttaga
    15301 tgacattatt gctgattatc aaaaaatggc cgatatgaat aatgaagttg taaaattatc
    15361 agaagagaat cataaactta aaaaagaatt agaagaatta agactacgtg tagcaacatc
    15421 tagacctcag gacaataaaa gtttttcttc gaataataca
//
LOCUS       DAPIDM010000054        15072 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000054 DAPIDM010000000
VERSION     DAPIDM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.054"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            120..1106
                     /locus_tag="RJE42_001448"
     CDS             120..1106
                     /locus_tag="RJE42_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDV5876872.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLQVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASNINPEIAKTIKANGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
     gene            1329..2093
                     /locus_tag="RJE42_001449"
     CDS             1329..2093
                     /locus_tag="RJE42_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5876873.1"
                     /translation="MKRLNEIVICISFLFLTIFIGGCGIGKEAEVKKSFEKTLSMYPI
                     KNLEDLYDKEGYRDDQFDKKDKGTWTISSEMANQKKGEALNIKGMVLKINRNTRSAKG
                     FYYVNAIKKDENGRPQDNQIEYPVKMVDNKIIPTKDIKDEKIKKEIESFKFFAQYGNF
                     KDLKNYKDGDISYNPEVPSYSVKYQLTNDDYNVKQLRKRYNIPTNKASELLLKGSGNL
                     DGSSVGYKKIEFTFVEKKGENTYYTANLHFKPSEDK"
     gene            2187..4424
                     /locus_tag="RJE42_001450"
     CDS             2187..4424
                     /locus_tag="RJE42_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5876874.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     VSGDIFIINHRDLYRFQLQQDGIICYIHFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFAESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKVNYKEITFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFHVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDNNFSLT
                     MHLDHYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHHLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDVTDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4575..5753
                     /locus_tag="RJE42_001451"
     CDS             4575..5753
                     /locus_tag="RJE42_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV5876875.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVTKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5755..7143
                     /locus_tag="RJE42_001452"
     CDS             5755..7143
                     /locus_tag="RJE42_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5876876.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDEK"
     gene            complement(7629..9290)
                     /locus_tag="RJE42_001453"
     CDS             complement(7629..9290)
                     /locus_tag="RJE42_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV5876877.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9615..11390)
                     /locus_tag="RJE42_001454"
     CDS             complement(9615..11390)
                     /locus_tag="RJE42_001454"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDV5876878.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DHASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGNYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            11741..12214
                     /locus_tag="RJE42_001455"
     CDS             11741..12214
                     /locus_tag="RJE42_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDV5876879.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGYAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            12476..14068
                     /locus_tag="RJE42_001456"
     CDS             12476..14068
                     /locus_tag="RJE42_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV5876880.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKNAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(14397..>15072)
                     /gene="spa"
                     /locus_tag="RJE42_001457"
     CDS             complement(14397..>15072)
                     /gene="spa"
                     /locus_tag="RJE42_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDV5876881.1"
                     /translation="KLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLK
                     DDPSVSKEILAEAKKLNDAQAPKEEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKED
                     NKKPGKEDGNKPGKEDGNKPGKEDGNGIHVVKPGDTVNDIAKANGTTADKIAADNKLA
                     DKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLA
                     GRRREL"
BASE COUNT         4980 a         2315 c         2731 g         5046 t
ORIGIN      
        1 aataattcct ataattaata ctctcaaata ttgtttaaat aagttttaaa attttacaca
       61 tatttttatt taaaaaagat gtataattaa tgcattaaat atagaaagaa gttgatatta
      121 tgaaaaagtg tattaagact ttgtttttaa gtatcatttt agtagtgatg agtggttggt
      181 atcattcagc acatgcgtca gattcgttga gtaaaagtcc agaaaattgg atgagtaaac
      241 ttgatgatgg aaaacattta actgagatta atataccggg ttcacatgat agtggctcat
      301 tcactttaaa ggatccagta aaatcggttt gggcaaagac tcaagacaaa gattacctta
      361 ctcaaatgaa gtcgggagtc aggttttttg atattagagg tagagcaagt gctgataata
      421 tgatttcagt tcatcacggc atggtttatt tgcatcatga attaggaaaa tttctcgatg
      481 atgctaaata ttacttgagt gcttatccaa acgaaacaat tgtgatgtct atgaaaaagg
      541 actacgatag cgattctaaa gttacgaaga catttgaaga aatttttaga gaatattatt
      601 ataataaccc gcaatatcag aatctttttt acacaggaag taatgcgaat cctactttaa
      661 aagaaacgaa aggtaaaatt gtcctattca atagaatggg gggtacatac ataaagagtg
      721 gttatggtgc tgacacgtca ggtattcaat gggcagacaa tgcgacattt gaaacgaaaa
      781 ttaataatgg tagcttaaat ttacaagtac aagatgagta taaagattac tatgataaaa
      841 aagttgaagc tgttaaaaat ttattggcta aagctaaaac ggatagtaac aaagacaatg
      901 tatatgtgaa tttcttgagt gtagcgtctg gaggtagcgc atttaatagt acttataact
      961 atgcatcaaa tataaatcct gaaattgcaa aaacgattaa agcaaacggg aaagctagaa
     1021 ctggttggct gattgttgac tatgcaggat atccgtggcc tggatatgat gatatcgtaa
     1081 gcgaaattat agatagtaat aaataaggat tcaataatga tattaagacg agtatgaaaa
     1141 tagttagatt ctaattattt tcactactcg tttttatttt gaaaataagt aataattcaa
     1201 caatattata aatcgaacag attgtttgtg aaatttttga taatattaaa gtgaaaaagt
     1261 gttataaatt gataaatata tgtaattaac aaaaacaaat catacaaaaa gaagagagtt
     1321 gtgatacggt gaagcgttta aatgaaatag tgatatgcat tagtttttta tttttaacaa
     1381 tttttatagg aggttgtggc ataggtaaag aagcggaagt taagaaaagc tttgaaaaaa
     1441 cattgagtat gtatccgatt aaaaatctag aagatttata cgataaagaa ggatatcgtg
     1501 atgatcaatt tgataaaaaa gataaaggca cctggactat tagttctgaa atggcaaatc
     1561 agaaaaaggg agaagcctta aatataaaag gtatggtttt gaagataaat agaaatacaa
     1621 gaagtgcaaa aggattttac tatgttaatg cgataaagaa ggatgaaaat ggcagacctc
     1681 aggataatca aatagaatat cccgttaaaa tggtagacaa taaaattata ccaactaaag
     1741 atatcaaaga tgaaaaaata aaaaaagaaa ttgaaagctt taagtttttc gcgcagtatg
     1801 gcaattttaa agatttgaaa aattataaag atggagatat ttcatataat ccagaggtgc
     1861 cgagttattc agttaaatat caattaacga atgatgatta taatgtaaag caattacgta
     1921 aaagatataa tataccaacg aataaagcat ctgagttatt attgaaaggt tcaggaaatt
     1981 tagatggttc atcagttgga tacaaaaaaa ttgaatttac tttcgtagag aaaaaaggag
     2041 aaaacactta ctatacggct aacctacatt ttaaaccaag tgaggataaa taatcatcca
     2101 cgcacacgat tcaatatgaa attcaaatat gttgctgtaa aataatgtaa aataaacgta
     2161 ttcatattac tgaattgagg gatagtatgc aacgtgatta tttaattcga gtagaaactg
     2221 aaagcatgcc agatttcaaa aggctcaatg gtttaatgat tggttttgtt attaaaggtg
     2281 aggcacatat ttatgatgaa aataatatga cacaatgcgt cagtggcgac attttcatca
     2341 ttaatcaccg cgacttgtat cgatttcaac ttcaacaaga tggcatcata tgttatatcc
     2401 acttccaaat gaaatattta gcagacaagt ttgatgatgt gcattgttta tattttcact
     2461 taacagatgc gaccacaaca aaaaatatac atcaactgag aaatataatg gcaagactgg
     2521 tttcaacaca cattcgacat aatgagttgt ctaaattgac tgagcaacaa cttgtgattc
     2581 agttgcttat gcatatgatt cattatgtcc cgcgtacata tcattcgaac caaagtatct
     2641 taaatgatga taaagtgaat caagtatgcg actatatcga gttacatttt catgaagact
     2701 taagcctttc ggaattaagc gaatatgttg ggtggtcaga gagccatctg tctaaaaagt
     2761 ttgcagaatc gctaggtgta ggattccagc atttcttaaa tacgacgcga attgagcatg
     2821 cgaaactcga tttgacatac acggatgaaa cgattactga tattgcattg caaaatggct
     2881 tttcaagtgc agcgagcttt gcgagaacat ttaaacactt tacgcatcaa acacctaaac
     2941 aatatcgagg tgatcgtcca gcaatcactg aaaatcaaca atcggcacaa cataattatc
     3001 acgaccgtga attgatatta cttttaaatg actacattga agaaatgaat catttcattg
     3061 aagatattga aaaggtgaac tataaagaga ttacctttca accaaccaat caccaactca
     3121 atcaatttaa tcatattatt caagtgggct atttgaggaa tttgctcaat acacagtatc
     3181 aatcacagtt gcttacatgt catcatgatt ttcatgtcaa tgaagtatta gcatatgatg
     3241 tgatgccata tattatgaaa aagctcaatg cgccattcac gtatgatgca gaaatttcga
     3301 atatatttta tgatatcgat ttgtgtttag actttttatt agataataac tttagtttga
     3361 ccatgcattt ggatcattat gactcacggg attatatcga tgcatttaaa atttttatcc
     3421 atcacgttgc cctgcatgtc agtcatagaa aagatttgaa gttcaacttg tatgtgacga
     3481 cattgcacac gtctttgatt gaaatgattg attattttaa agcattattc cctaatggtg
     3541 gcttgtacat tcacttagat caagctacgg aaagacatct accattgttg aaacgacttg
     3601 agccacacat cgaccatttt gtatttgatg ccaattcaaa tgatgctgtt gattttaata
     3661 aaatgaatga tgatgaattt aaaaccgcaa gtcaaatgat tattaataaa acgaattacc
     3721 ttatcgattt aatgcatcgt catcacctaa agcgtccact cattttactc aattggaata
     3781 cattgacggg tgatacattt atcacaaacg gtgaatattt tagaggtggt atcatcattg
     3841 agcaattatt aaaattaagt tctaaagtag agggtatcgg gtattggttg aattatgatt
     3901 tgcacgtcag tcattgtaga aatgaacggg attatatgaa ttctattgaa ctgtttcatc
     3961 aatataatgg aaaacgtccg gtctatttca cggcattgct atttaataaa ttaacaagca
     4021 atattttgta ttctgatgat acatgtattg tcacgggaac tgattcaaat tttcaaatat
     4081 tgttatatga tgcaaagcat tttaatccgt acttagcgtt ggacaatcaa atgaatatgc
     4141 gtgcaacgga aatgatccat ttgaacatta atgccctaga agacggtatg tataagatta
     4201 aacattttac cttagataaa gaaaatggtg cgttatttaa tctttggcgc aaacatcata
     4261 cgatacatgg catggacaag gactctatag attacgttaa tcgaatgagt tttccgaaat
     4321 tagaagtgta cgatatagat gtgacggata cactggcatt aaacattaaa atgattacga
     4381 atgggattca cttaattgaa gtaaaacgtt acccaagttc ataaaatgat cacaaatcac
     4441 aaattttgat atacataatt tgtgattttt tgtattcaaa gttaaaattg caaaaaatta
     4501 atggttaaca tctctgttgt tggcaatata aataatgatt aatcatttat gatgtaacta
     4561 aggagatgaa ggatatgaat caacaattaa ttgaaacttt aaaatctaaa gaaggcaaaa
     4621 tgattgagat cagacgttat ttacatcagc atccagaatt atcttttcat gaggatgaaa
     4681 cggcgaaata catcgctgaa ttttacaaag gtaaagatgt agaagtagaa acgaatgtcg
     4741 gaccacgtgg aattaaagta acgattgatt cagggaaacc tggtaaaaca ttagcaattc
     4801 gtgcagactt tgatgcatta cctattactg aagatacagg attatctttt gcatcacaaa
     4861 ataaaggtgt tatgcacgca tgtggtcacg atgcacatac agcatacatg cttgtattag
     4921 cagagacgct tgctgaaatg aaagatagtt ttacaggaaa agtcgttgtg atacatcaac
     4981 cagctgaaga agtaccacca ggtggtgcta aagcaatgat tgaaaatggt gtattagacg
     5041 gtgttgatca tgtattaggt gtacacgtca tgagcacaat gaaaacaggt aatgtgtact
     5101 acagacctgg ttatgttcaa acaggacgcg cattcttcaa attgaaagtt caaggtaaag
     5161 gtggccatgg ttcatcacca catatggcca atgatgccat tgttgcaggt agctacttcg
     5221 tcacagcgtt acaaacagtt gtgtctagac gactaagtcc atttgaaaca ggtgttgtca
     5281 caatcggttc atttgacggt aaaggtcaat tcaatgtcat taaagatgtt gttgaaattg
     5341 aaggtgatgt acgtggatta acagatgcta caaaagcaac aattgaaaaa gaaattaaac
     5401 gtttatcaaa aggattagaa gcattgtatg gtgtaacttg cacattagaa tataacgatg
     5461 attatcctgc attatataat gatccagagt ttactgagta cgtgactaag acgctaaaag
     5521 aagcagacct tgaatttggt gtcgaaatat gtgaaccaca accaccttca gaagactttg
     5581 catactatgc taaggaacga ccaagtgcct ttatttatac aggtgcagct gtggaagatg
     5641 gtgaaattta cccacatcat catcctaaat ttaacatttc agaaaaatca ttacttattt
     5701 cggcagaagc tgtagggaca gttgttttag attaccttaa aggagataac taacatgaat
     5761 gaaacgtatc gcgggggcaa caagttaatc ttaggtattg tattaggtgt tattacattt
     5821 tggttgtttg cacaatcact tgtaaatgtt gtaccaaatt tacaacaaag ttttggtgca
     5881 gacatgggaa caattagtat tgcggtaagt ctaaccgcac tattttcagg catgtttgtt
     5941 gttggagcag gcggcctagc agataaaatt ggacgcgtga aaatgacgaa tatcggctta
     6001 ttattaagta ttattggttc agcattaatt attattacaa atttaccagc attgctaatt
     6061 ttaggtcgta ttatacaagg cgtatcagca gcgtgtatta tgccttccac attggccatt
     6121 atgaaaactt attatgaggg tgctgaacgt cagcgtgcct taagctattg gtctatcggt
     6181 tcttggggtg gaagtggtat ctgttcactc ttcggtgggg cagttgcgac aactatgggt
     6241 tggagatgga ttttcatctt ctcaattatc gttgccgtac tttcaatgtt actcatcaaa
     6301 ggaacgcctg aaacgaaatc agaagttacc aatacacata aatttgacgt tgcaggtcta
     6361 attgttctag tagttatgtt attaagttta aacgttgtta ttaccaaagg tgcagcactt
     6421 ggttatacat cattatggtt ctttggtttg attgcaatcg taattgtagc attctttatt
     6481 ttcttaaatg ttgagaaaaa agtagataat ccacttattg attttaaatt atttgaaaat
     6541 aaaccatata caggtgcaac gatttcgaac ttcttattaa acggtgttgc aggtacatta
     6601 attgtagcga atacattcgt gcaacaaggt ttaggttata cagcattgca ggcaggatac
     6661 ttatcaatta cttatttaat catggtgtta ttgatgattc gagttggtga aaaattatta
     6721 caaaaaatgg gttctaagcg accaatgtta ttaggtacat tcattgtggt cattggtatt
     6781 gcacttattt cattagtatt cttaccaggc atattttatg ttatcagttg tgtcgtagga
     6841 tatttatgtt tcggactagg cttaggtatt tatgcaacac cttctacaga tacagctatt
     6901 tcaaatgcac cgttagataa agttggcgtt gcttcaggta tttataaaat ggcttcatca
     6961 cttggtggtg cattcggtgt cgcaattagt ggtgcagtat atgccggtgc agttgctgca
     7021 acgagcattc atacaggtgc gatgattgca ctttgggtta acgtattaat gggaatcatg
     7081 gcatttatcg caattttatt cgcgattcct aatgatgata aacgtgtcaa agatgagaaa
     7141 taataacgat gacacgccaa gtgttttgat aatgatataa atgaaagtaa agcaaattaa
     7201 atgaggaaat gtatagagca gtgcatatag ataaatctat gattggatta acactaatat
     7261 aaatataaat gaaaaaccat ccacttaaac ttttgataag ttaggtggat ggttatttat
     7321 tgcgttgttc taaaaacttg gacacttata tggaaagaac tatatataat gtgaaggtta
     7381 tggcaattcg cttcaatgaa ggcgacgtgt aaaagtggat attaaacgtt tcggggtcag
     7441 actccgagaa cagtgtccgt catgcttacc gcatactaat gaagcattat tatatcagtg
     7501 tttatagccg atttaagata ataaagtttg ctaaacaaaa aagccaatcc attgatgttg
     7561 gattgacttt ttacatgcat ctgaatctct aattttaaaa aatatgaata taaataagac
     7621 attaaaagtt aaatttcagt tgttgcaatt tcttcatctg taggtacatc atcgttaaga
     7681 ccaacaagtg cttcagaaac atttcgtgaa tgataaccga tacgttcaag aacaccaatc
     7741 atatcgatat atagtaatcc gccttttgtt gtacattcac cacgattaag gcgtttaata
     7801 tgacctttgc gtagtttatg ttcaatatta aatgattctc tactacgttc tacaatttca
     7861 tcttttttcg ttttgtcata aacatctaac atgtcaatgg ctttatcaaa tgactcagca
     7921 acatggttga ataatttatc cataccgcgt tgtgcatctt ctgtaatgcg aatatcttca
     7981 tcatgttggc gttttaattg agcgacatac tcttctgtta gctctgctac ttttaaaata
     8041 gagcgattga catcaaacat aactgctaaa cgctcaacgt ctgccttcgt aatggctttt
     8101 gtagaaattc taactaaata atttcgaatg ctatcattga ttgtttcaac agcttggtgc
     8161 ttttgttcaa gctttttgat caatttttta tcgtcttttg taatttcgcg aatgtcttca
     8221 aacattgata agacaatctg acccacattt tgtaattctt tttgagtttc ttgtaatgca
     8281 acaccaggtg cgtgataaac aagatctttg tttaagtgct gaggtttata gtcatcagca
     8341 atatctttac ctgggacaag ctttgtaact atccatgcta aacctgctac aaatggtaat
     8401 tgaatcaaag tatttgttat gttgaagata ccatgtgata ctgcaatcgt catcgctggt
     8461 tttaagtgcc atacatcttg taacaaacta atcaaatgaa ttacaactgg caagaaaatt
     8521 gtgaagataa tcaccccgat taagttaaag atgacgtgta caagcgccgc acgttttgca
     8581 gcgattgagc cggctaaact agctaagata gctgtaatcg ttgtaccaat attatcgcct
     8641 agtaacacag ggattgctgc gtttaagcta attaaatctt gttgataaaa ttcttgtaaa
     8701 ataccaatcg tcgcacttga actttgaact agtgctgtta accctgcgcc gacaatgaca
     8761 gcaagtattg gatttgtaga catatcaagc attaattgct taaatccatc taatgatgct
     8821 aaaggtttaa cggcatcacc cataaattct agaccgaaga atagagaacc gaaaccgaat
     8881 agtatgcggc caatgttatt gattttagag cgtttaaaga aaaagattaa aaatgcacct
     8941 aatgctaaaa ttggcattgc atattcgcct aaatctatac cgataataaa tgcagttacc
     9001 gttgttccga tattagcacc cattatcact ccaatggctt gtttcaatgt cataaatcca
     9061 gctgttacca gtccgattgt gataactgtc gtacctgaac tactttgtat taaaatagtt
     9121 acaacgatac ctgcaataac acctaatact ggatttgatg taaatttgtt taaaatatct
     9181 cgtagcctgt ctcctgctga tgcttgaagc ccgtctccca tgatttttaa gccgtaaagg
     9241 aaaataccta aaccacctaa aaaggagaaa atgacttctg taaccgacat ttccattatt
     9301 tcacctcaaa taagctttat atttagatta tcgcttataa ttgtaaattt aatgttaaga
     9361 ttaggtaaaa ttatttaaca atatatgtta tttgtatatg acttgtaaaa tatcgtcact
     9421 tattatgtaa attttcagtg tgaaatggca ggtttgcaat cacttgttta acaaaatgat
     9481 gcaatcaatc atgcaattat gtgtcatcaa aaaaatcatg tgagtgggat aacgaaataa
     9541 agtttgtgaa tatatcattt ctatcccact ccatgatttg aaatcaccat atagacatga
     9601 tttaatgtgc ctcgttataa caatttgtgt tcttttaata atgactcaat gtacgtacct
     9661 tttatctttt taaggaatcc tgctaatgca agtttttgca ttttcgaatc tttagtaatc
     9721 tcgcgcaaat cttgatggtc attcagttcg tatacggcat ccattaagac gcgaagatca
     9781 aatgtactat tgatgacctc tggaatacca cgatctatat ttagtaattg ataaacagct
     9841 tccatggcag tacgaaccga atattctgtt gtaaatacag tatctcgctc tgtttctgca
     9901 aagttaccaa taaatgctaa attttgtgat tgatgcggga cgactaaagg tctgtcgccg
     9961 atagcacgcg tcatgaaata agaggtaata tatggcatat aaacaggaat cgtattagat
    10021 gcatgttttg ctaagtcttc aattttgtca gttggtacac ctaagtgata tagccattct
    10081 tgacatattt cattaccact acattctgtg attggctttt taatataatt gccatttaca
    10141 tctgaatata aggcataaat ccacgtagat atttcatttt taggttggtc tttaaactgt
    10201 tgctgacgat tgattgtaaa actgatttgc catgcagaat cattgattgt aataataccg
    10261 ccagtaaccg ttttacctgc aagtggatca cgtttacaaa tgctttctat tgtatcgata
    10321 atatctttat tgtttgttgt cgatgttgct gaaacaaacc aacttttttt aggaatattt
    10381 tggcaaaact tatcaggatt accaaattca ggactttgtc gcgctaaatt tttccatagt
    10441 gtccaactac cacctaattc gtcagttggt ggcgctggtg tatcattatc accataagta
    10501 gagctttctg taatactacc gtttgtaaca aagacaagat cgtttatagt cagtttaata
    10561 gattctgcat taccattacg gtcaattaat atttctcggg caattttttg acttgtcgta
    10621 acatctactt taatatcttc tacttttaca tcgtattcaa attgaacccc atgcgatttt
    10681 aaatattcaa ccataggtaa tactaaagat tcatattgat tatatttagt gaattttaaa
    10741 gctgaaaagt ctgcgagacc accaatatga tgaacgaatc gcattagata gcgacgcatt
    10801 tccattgcag aatgccacgg ttcaaatgca aacatcgttt tccagtaaat ccaaaagttt
    10861 gaattaaaga aatcgtcgga aaatacatct gttattttga catcatctaa atcttcttca
    10921 ttcgttaagc ataaatctaa aatttcttta atcgccgttt tagtcaaagt gaagtctcca
    10981 tctgtgacta aacgttgacc ctgtttctca ataacacgac aacgagaata gttagggtct
    11041 tctttgttta accaatagaa ttcatctaat actgacgcat gatcaatttc aagtgaaggg
    11101 atagatctga ataaatccca caaacattca aagtggttct ccatttcacg accacctcgg
    11161 acaacatagc cttttaaagg catattttca ccatcaagac taccgcccgc tttgggtaac
    11221 tcttctaaaa tatgaatctt agaaccttcc atttgaccat cccttattaa aaaacaagct
    11281 gcagcaagtg aagctagacc agatccgatt aagtaagcgg atttgttttc tacattttca
    11341 ggttttttag ggcgcgcaaa tgcttcataa tttccataac tgtaatacat atccaccaag
    11401 tcctttcgta ttagaataca ctcagactat acccctttgt gaattgcaaa tattagtatt
    11461 tatattggta aatttgacga aattagatca tttttatatt tttgattttt aaaattttat
    11521 cactcgaatg tttttggatt ctgaggtgcg ggacttggaa taattcgaat cattatgtgg
    11581 catgttaaac agctaaatat aagctatgca catttaacaa gcggatactc ctgacgcaat
    11641 tatagtatca ttacatattg tttaatcgtt tggggtaatg taattaaaag gtaaaatagg
    11701 cagagtgtta tacataaaat ttaaagaaag aggcgataga atgtctaaaa tcaggtcttt
    11761 tacaatatta agtctactta tttacttagc tatgatgtgc tatacagtag tgacctattc
    11821 aaaattacca accaaagtac ctattcacta taatttagca gggtatgctg ataactttgc
    11881 tgataaatgg gttttgcttt tgattaatag cgcatttata gtgatttggc ttatattttt
    11941 cattgcaggt agatactatg aacgatttgc caaatggtca cattataatc atacaccacg
    12001 tgaaattcga gcgattaaat tatttttaag tacgttaaat ttagagatta tgagctatat
    12061 gtctatcttc acagtattag aaatttggca aatacaacac catcatcaat tcaatttact
    12121 atggtttaat atgatattta tcattatcat tggtttgacg cttgtcatat tttgtttact
    12181 tccgacaatt cataaaatga gagattctca ataaaaatat aaactgtcat gatacagttt
    12241 tgaagcggat tcctgagatt attataaata atatctagta tttataatta aaatgatata
    12301 ttatttatta tagttaaata ttgtgtatat tttgtgaact ttttgtaaaa attcgattgc
    12361 ctgtcacata taggagtgtt acattttaaa tatgtgatca tcgcaaaata taagttgaaa
    12421 taggttgtag attaatcagg atgataaata ttttatataa agagagggag tcattatgac
    12481 attacttact gtaaatccat tcgataatgt tggattatca gccttagttg cagcagtacc
    12541 tattatttta tttttattat gcttaaccgt ttttaaaatg aaaggcattt atgcagcatt
    12601 gacaactttg gttgttacat tgattgtggc tttatttgta tttgaattac cagcgcgtgt
    12661 atcagcaggt gcgattacag aaggcgttgt tgccggtatt ttcccaatag gatatatcgt
    12721 tttaatggca gtttggttat ataaagtttc tattaaaaca ggacaatttt ctattattca
    12781 agatagtatt gcaagtattt cagaggacca aagaatccaa ctattattaa ttggattttg
    12841 tttcaatgca tttttagaag gtgcagcagg atttggtgtg ccaattgcga tttgtgcagt
    12901 attattaatt caacttggat ttgaaccatt aaaagcagcg atgttatgtt taattgctaa
    12961 tggtgcggcg ggtgcctttg gtgcaattgg tttaccagtt agtattattg atacgtttaa
    13021 cttaagtgga ggcgttacaa cattagatgt tgcgagatac tcagcattaa cacttccaat
    13081 tttaaacttt attattccat ttgttttagt attcattgta gatggtatga aaggtattaa
    13141 agaaatttta cctgtcattt taacagtgag tggtacatat actggattac aattattatt
    13201 aacaatattc catggtccag aactagcaga cattattcca tcactagcaa caatggtggt
    13261 gttagcattt gtttgtcgta aatttaaacc gaaaaacatt ttcagattgg aagcgtctga
    13321 acataaaatt caaaaacgaa cgcctaaaga aattgtcttt gcttggagtc cgtttgtcat
    13381 tttaactgcc tttgtattag tatggagtgc accattcttc aaaaaattat tccaacctgg
    13441 aggtgcactt gaaagtctag taataaaatt gccaattcca aatactgtga gtgatttatc
    13501 gcctaaagga attgcgttgc gtctcgattt aattggtgca actgggacag cgattttatt
    13561 aacagtaatt attacaattt taattacgaa gttaaaatgg aaaaatgcag gtgctttatt
    13621 ggtcgaagca attaaagaat tatggttacc gatccttaca atttcagcta tcctagctat
    13681 tgctaaagtt atgacatacg gtggtttgac tgtagcaatt ggacaaggta ttgctaaagc
    13741 gggagcaatt ttcccattat tctctccagt attaggttgg attggtgtgt ttatgactgg
    13801 ttcagttgta aataacaata ctttattcgc acctattcaa gcgacagtgg cacaacaaat
    13861 ttcaacaagc ggttcattac ttgtggcagc taacactgca ggtggtgtag cagcgaaact
    13921 tatttcacca caatcaattg ccattgcgac tgcagctgtt aaaaaagttg gtgaagaatc
    13981 tgcattatta aaaatgacgt taaaatacag tattatattt gttgccttta tttgtgtttg
    14041 gacgtttata ctaacgttaa tattctaaat ataaataatg ttgtcacttg gattcaaatg
    14101 acattttaaa tctaattatt catgaatcga actagtacga aatgcaatga gcatcttgtc
    14161 tagttcgatt ttttaatgtc taaaaatgtc gtatatataa tcagagtaga aagtgttgag
    14221 gcgtttcaga agttgtttag aaaagtaagt aaaataaaaa atgcactgag caacaaaaga
    14281 tgttgctcgt gcatttagat gattcttatc atttcaaata agaatgtgtt aatcaacgta
    14341 tataagttaa aattggtttg gataaaatga tatctatcgt tgtgtattgt ttgtttttat
    14401 agttcgcgac gacgtccagc taataacgct gcacctaacg ctaatgataa tccaccaaat
    14461 acagttgtac cgatgaatgg attttcttca ccagtttctg gtaatgcttg agctttgtta
    14521 gcatctgcat ggtttgctgg ttgcttctta tcaacaacaa gttcttgacc aggtttgatc
    14581 atgtttttat cagctaattt gttatctgca gcaattttgt cagcagtagt gccgtttgct
    14641 tttgcaatgt catttactgt atcaccaggt ttaacgacat gtattccgtt gccgtcttct
    14701 ttaccaggtt tgttgccatc ttctttacca ggcttgttgc catcttcttt accaggtttt
    14761 ttgttgtctt ctttaccagg tttgttgcca tcttctttgc caggtttttt gttgtcttct
    14821 ttaccaggtt tgttgccgtc ttctttacca ggcttgttgt tgtcttcctc ttttggtgct
    14881 tgagcatcgt ttagcttttt agcttctgct aaaatttctt tgctcactga aggatcgtct
    14941 ttaaggcttt ggatgaagcc gttacgttgt tcttcagtta agttaggtaa atgtaaaatt
    15001 tcatagaaag cattttgttg ttctttgttg aatttgttgt cagcttttgg tgcttgtgca
    15061 tcatttagct tt
//
LOCUS       DAPIDM010000055        14975 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000055 DAPIDM010000000
VERSION     DAPIDM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.055"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..704
                     /gene="dprA"
                     /locus_tag="RJE42_001458"
     CDS             <1..704
                     /gene="dprA"
                     /locus_tag="RJE42_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV5876882.1"
                     /translation="YAFIKLDHNDIIKELQKLKVSYITYMDTEYPVLLKEIYQFPLLL
                     FYKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAH
                     QIALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERN
                     RIISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGA
                     KVVLNANDIFEDYYI"
     gene            878..2953
                     /gene="topA"
                     /locus_tag="RJE42_001459"
     CDS             878..2953
                     /gene="topA"
                     /locus_tag="RJE42_001459"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV5876883.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITNKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIERDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3109..4416
                     /gene="trmFO"
                     /locus_tag="RJE42_001460"
     CDS             3109..4416
                     /gene="trmFO"
                     /locus_tag="RJE42_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDV5876884.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            4833..5729
                     /gene="xerC"
                     /locus_tag="RJE42_001461"
     CDS             4833..5729
                     /gene="xerC"
                     /locus_tag="RJE42_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV5876885.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDASKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNNIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            5726..6271
                     /gene="hslV"
                     /locus_tag="RJE42_001462"
     CDS             5726..6271
                     /gene="hslV"
                     /locus_tag="RJE42_001462"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV5876886.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            6337..7740
                     /gene="hslU"
                     /locus_tag="RJE42_001463"
     CDS             6337..7740
                     /gene="hslU"
                     /locus_tag="RJE42_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDV5876887.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            7765..8538
                     /gene="codY"
                     /locus_tag="RJE42_001464"
     CDS             7765..8538
                     /gene="codY"
                     /locus_tag="RJE42_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDV5876888.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            8589..8681
                     /locus_tag="RJE42_001465"
     CDS             8589..8681
                     /locus_tag="RJE42_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876889.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            8880..9647
                     /gene="rpsB"
                     /locus_tag="RJE42_001466"
     CDS             8880..9647
                     /gene="rpsB"
                     /locus_tag="RJE42_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV5876890.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            9681..9794
                     /locus_tag="RJE42_001467"
     CDS             9681..9794
                     /locus_tag="RJE42_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876891.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            9829..10710
                     /locus_tag="RJE42_001468"
     CDS             9829..10710
                     /locus_tag="RJE42_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV5876892.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            10847..11569
                     /locus_tag="RJE42_001469"
     CDS             10847..11569
                     /locus_tag="RJE42_001469"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV5876893.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            11588..12142
                     /gene="frr"
                     /locus_tag="RJE42_001470"
     CDS             11588..12142
                     /gene="frr"
                     /locus_tag="RJE42_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV5876894.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            12515..13285
                     /locus_tag="RJE42_001471"
     CDS             12515..13285
                     /locus_tag="RJE42_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDV5876895.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            13292..14074
                     /locus_tag="RJE42_001472"
     CDS             13292..14074
                     /locus_tag="RJE42_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV5876896.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            14286..>14975
                     /locus_tag="RJE42_001473"
     CDS             14286..>14975
                     /locus_tag="RJE42_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDV5876897.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKY"
BASE COUNT         5607 a         2091 c         2874 g         4403 t
ORIGIN      
        1 actatgcttt tataaaactt gatcataacg atattattaa agaactgcag aaattaaaag
       61 taagttacat tacatatatg gatactgaat acccagtgct attaaaagaa atatatcaat
      121 ttccattact tcttttctat aaagggaaca tcaaattaat aaataatatg catcatttgg
      181 ctgtagtagg tgcaagagat tctacaagtt atacccaaca gtctttagaa tttttattat
      241 caaatgataa aagcaaatat ttaacaattg tttccggcct tgctcaagga gctgatgcaa
      301 tggcacatca aatagcttta aaatacaatc tccctacaat agcagtttta gcctttggcc
      361 atcaaacaca ttatcccaaa agtacattag cattaagaaa taaaatagaa gaaaaaggtt
      421 tagttatatc cgaatatcca ccacatacac caattgctaa atatagattt cctgagcgca
      481 atagaattat cagcggtttg tcaaaagggg ttttaattac tgaggctaag gaacaaagtg
      541 gcagtcacat cacgatagat tttgcattag agcaaaatag aaatgtttat gttttacctg
      601 gatctatgtt taatcctatg acaaaaggta atttattacg tatccaagaa ggtgctaagg
      661 tagtattaaa cgctaatgat atatttgaag actactatat ttaaaactaa aaataacgag
      721 ttgcttcata tttcttttgt taccctttgt aatttatttt ttaataaaat ctataaaaaa
      781 tagacaggga aaatgatttg tttagatata aaacgttgac aaaagcaaaa ttaagcgttt
      841 atcatttatc tttagtaatt agattagcga gggggaaatg acattggcag ataatttagt
      901 cattgttgaa tcgcctgcaa aagcaaaaac cattgaaaag tatttaggta agaagtataa
      961 agttatagct tcaatgggac acgtcagaga cttaccaaga agtcaaatgg gtgtcgacac
     1021 tgaagataat tacgaaccaa aatatataac aatacgcgga aaaggtcctg ttgtaaaaga
     1081 attgaaaaaa catgcaaaaa aagcgaaaaa cgtctttctc gcaagtgacc ccgaccgtga
     1141 aggtgaagca attgcttggc atttatcaaa aattttagag cttgaagatt ctaaagaaaa
     1201 tcgcgttgtt ttcaacgaaa taactaaaga cgctgttaaa gaaagtttta aaaatcctag
     1261 agaaattgaa atgaacttag tcgatgcaca acaagcgcgt cgaatattag atagattggt
     1321 tggctataac atctcgccag ttctatggaa aaaagtaaaa aaagggttgt cagcgggtcg
     1381 agttcaatct gttgcacttc gtttagtcat tgaccgtgaa aatgaaatac gaaactttaa
     1441 accagaagaa tattggacta ttgaaggaga atttagatac aaaaaatcaa aattcaatgc
     1501 taaattcctt cattataaaa ataaaccttt taaattaaaa acgaaaaaag atgttgagaa
     1561 aattacagct gcattagatg gagatcaatt cgaaattaca aacgtgacta aaaaagaaaa
     1621 aacgcgtaat ccagcaaacc catttacaac ttctacatta caacaagagg cggcacgtaa
     1681 attaaacttt aaagctagaa aaacaatgat ggtcgcacaa caattatatg aaggtataga
     1741 tttgaaaaaa caaggtacga ttggtttaat aacatatatg agaaccgatt ctacacgtat
     1801 ttcagatact gccaaagctg aagcaaaaca gtatataact aataaatacg gtgaatctta
     1861 cacttctaaa cgtaaagcat cagggaaaca aggtgaccaa gatgcccatg aggctattag
     1921 accttcaagt actatgcgta cgccagatga tatgaagtca tttttaacga aagaccaata
     1981 ccgattatac aaattaattt gggaacgatt tgttgctagt caaatggctc cagcaatact
     2041 tgatacagtc tcattagaca taacacaagg tgacattaaa tttagagcga atggtcaaac
     2101 aatcaagttc aaaggattta tgacacttta tgtagaaact aaagatgata gtgatagcga
     2161 aaaggaaaat aaactgccta aattagagca aggtgataaa gtcacagcaa ctcaaattga
     2221 accagctcaa cactatacac aaccacctcc tagatatact gaggcgagat tagtaaaaac
     2281 actagaagaa ttgaaaattg ggcgaccatc aacttatgca ccgacaatag atacgattca
     2341 aaagcgtaac tatgtcaaat tagaaagtaa gcgttttgtt cctactgagt tgggagaaat
     2401 agttcatgaa caagtgaaag aatacttccc agagattatt gatgtggaat tcacagtgaa
     2461 tatggaaacg ttacttgata agattgcaga aggcgacatt acatggagaa aagtaatcga
     2521 cggtttcttt agtagcttta aacaagatgt tgaacgtgct gaagaagaga tggaaaagat
     2581 tgaaatcaaa gatgagccag ccggtgaaga ctgtgaagtt tgtggttctc ctatggttat
     2641 aaaaatgggg cgctatggta agtttatggc ttgctcaaac ttcccggatt gtcgtaatac
     2701 aaaagcgata gttaagtcta ttggtgttaa atgtccaaaa tgtaatgatg gtgacgtcgt
     2761 agaaagaaaa tctaaaaaga atcgtgtctt ttatggatgt tcgaaatatc ctgaatgcga
     2821 ctttatctct tgggataagc cgattgaaag agattgtcca aaatgtaacc aatatcttgt
     2881 tgaaaataaa aaaggcaaga caacacaagt aatatgttca aattgcgatt ataaagaggc
     2941 agcgcagaaa taatattttt atttcctaga gacattttaa gattgttaaa tagaatcatt
     3001 agtgaatctt attttaaaga tagtaatgga ttaatctaaa taagtgcgga taatataaac
     3061 ataacaacat aattaaaaga cataaatgac aataaaagga gtatagaaat gactcaaact
     3121 gtaaatgtaa taggtgctgg tcttgccggt tcagaagcgg catatcaatt agctgaaaga
     3181 ggaattaaag ttaatctaat agagatgaga cctgttaaac aaacaccagc gcaccatact
     3241 gataaatttg cggaacttgt atgttccaat tcattacgcg gaaatgcttt aactaatggt
     3301 gtgggtgttt taaaagaaga aatgagaaga ttgaattcta taattattga agcggctgat
     3361 aaggcacgag ttccagctgg tggtgcatta gcagttgata gacacgattt ttcaggatat
     3421 attactgaaa cactcaaaaa tcatgaaaat atcacagtta ttaatgaaga aattaatgcc
     3481 attccagatg gatacacaat tatcgcaaca ggaccactta ctacagaaac ccttgctcaa
     3541 gaaatagtgg acattactgg taaagatcaa ctttatttct atgatgcggc tgctccaatt
     3601 attgaaaaag aatctattga tatggataaa gtttacttaa agtcccgtta tgataaaggt
     3661 gaagctgcat atttaaactg tcctatgact gaggatgaat ttaatcgctt ttatgatgca
     3721 gtattagaag ctgaagttgc gcctgtaaat tcatttgaaa aagaaaaata tttcgagggt
     3781 tgtatgcctt ttgaagtaat ggcagaacgt ggacgcaaga cattactatt tggaccaatg
     3841 aaaccagtag gattagaaga tccaaagacg gggaaacgtc cttatgcagt ggttcaatta
     3901 agacaagatg acgctgctgg cacactctac aatattgttg gcttccaaac gcatttaaaa
     3961 tggggagctc aaaaagaagt tattaaatta attccaggtt tagaaaatgt tgatattgtt
     4021 agatatggtg tgatgcatag aaataccttc attaactcac cggacgtatt aaacgagaaa
     4081 tatgaattga tttcacaacc aaacatacag tttgcgggac aaatgactgg tgttgaaggt
     4141 tatgtagaaa gcgcagctag cggcttagtt gcaggcatca atcttgcgca taaaatatta
     4201 ggcaaaggtg aggtagtatt tccgagagaa actatgattg gaagtatggc ttactatatt
     4261 tctcatgcta aaaacaataa gaatttccaa cctatgaatg ctaacttcgg gttattacca
     4321 tctttagaaa ctagaattaa agataaaaaa gaacgctatg aagcacaagc taatagagct
     4381 ttggattact tagaaaattt caaaaaaact ttataaaata gttagaaaga ctagatatgc
     4441 tattcattct taagtcatca acgagtaagt aatgactttc taaatggaaa atacttatcc
     4501 tagtcttttt aattttggaa ttgttacgta tttctgacaa ttgagaattc gtattcaaaa
     4561 aatatctaaa taaataacac gcaataagtt gattgatgta acatgtaaga gaatgtttta
     4621 aataaacttt atttaaaaga caacgaaata ataaatggca aggctattaa taaagacttt
     4681 tagtaattaa tttaaaaaag aggtattcta attaacaggt tttccgatta gttacaatta
     4741 tttaattctc aaaagattta gaattgatta tcaaattact gtaagccctt tgctgtatat
     4801 gctacaattc ttattgatgg agggtaaatg tattgaatca tattcaagaa gcgtttttaa
     4861 atacattgaa agttgaacgg aatttttcgg aacatacatt gaaatcatat caagatgact
     4921 taattcagtt taatcaattt ttagaacaag aacatttaca gttgaatact tttgaataca
     4981 gagatgctag aaattatttg agctatttat attcaaatca tttgaaaaga acatctgttt
     5041 ctcgtaaaat ctcaacgtta agaactttct atgaatattg gatgacgctt gatgagaaca
     5101 ttattaatcc atttgttcaa ttagtacatc cgaaaaaaga aaaatatctt ccgcaattct
     5161 tttacgaaga agaaatggaa gcgttattta aaactgtaga agaggacgct tcaaaaagtt
     5221 tacgggatcg agttattctt gaattgttat atgctacagg tatccgtgtt tcggaattag
     5281 ttaatattaa aaaacaagat atagattttt acgcgaatgg tgttaccgta ttaggaaaag
     5341 ggagcaaaga gcgctttgta ccgtttggtg cttattgtag acaaagcatc gaaaattatt
     5401 tagaacattt caaaccaatt cagtcatgca atcatgattt tcttattgta aatatgaagg
     5461 gtgaagcaat cactgaacgc ggtgtacgat atgttttaaa taatattgtt aaacgaacag
     5521 caggcgtaag tgagattcat ccccacaagc tcagacatac atttgcaacg catttattga
     5581 atcaaggtgc agacctaaga acagtacaat cgttattagg tcatgttaat ttgtcaacaa
     5641 ctggtaaata tacacacgta tctaaccaac aattaagaaa agtgtatcta aatgcacatc
     5701 ctcgagcgaa aaaggagaat gaaacatgag taatacaaca ttacatgcaa caacaattta
     5761 tgctgtaaga cataatggga aagcagctat ggctggagat gggcaagtaa cgcttggtca
     5821 acaagtcatc atgaaacaaa cggcaagaaa agtgcgacgt ttatatgaag gtaaagtgtt
     5881 agctggtttc gcaggtagtg tagcagatgc gtttacgtta tttgaaaaat tcgaaacaaa
     5941 attacaacag tttagtggta acttagaaag agctgctgtt gaattggcac aagaatggcg
     6001 aggcgataaa caattacgtc aattagaagc tatgctaatt gtaatggata aagatgctat
     6061 tttagttgtc agtggaactg gcgaagttat tgccccagat gatgacctta tcgctattgg
     6121 atcaggaggc aactacgcat taagcgcagg acgtgcattg aaacgccatg catcgcattt
     6181 gtctgctgaa gaaatggcat atgagagctt gaaagtagcg gctgatattt gtgtctttac
     6241 caacgataat attgttgtcg aaacactata ataatcagag aacgataaat aattacgagc
     6301 aattaatttt agttaaaaga cggaggaatg aaattaatgg atacagctgg aataagatta
     6361 actccaaaag aaatcgtatc taaattaaat gaatacatcg ttggacaaaa tgatgctaaa
     6421 cgtaaagtgg caattgccct acgtaatcga tacagaagaa gtttattaga tgaggaatca
     6481 aagcaagaaa tttcacctaa aaatattttg atgattggac caaccggcgt tggtaaaact
     6541 gaaattgcaa gaagaatggc caaagttgtc ggcgcgccat ttataaaagt agaagctact
     6601 aaatttactg aggtaggtta tgtaggacga gatgttgaaa gtatggttag agatcttgtt
     6661 gatgtttcag taagattagt caaggcgcag aaaaaatcat tggtacaaga tgaagcaaca
     6721 gctaaggcca atgaaaaact tgttaagtta ttagttccaa gtatgaaaaa gaaagcgtct
     6781 caaacgaata atcctttaga gtcacttttc ggaggtgcaa ttccaaattt cggacaaaat
     6841 aacgaagatg aagaagaacc acctactgag gaaattaaaa caaaacgttc tgaaattaag
     6901 agacaattag aagaaggcaa acttgaaaaa gaaaaggtaa gaattaaagt cgaacaagat
     6961 cctggtgctt taggtatgct aggtacaaat caaaatcagc aaatgcaaga gatgatgaat
     7021 caattaatgc ctaaaaagaa agttgagcga gaagttgctg ttgagacggc aaggaaaatc
     7081 ttagctgata gttatgcgga tgaactaatt gatcaagaaa gcgctaacca agaagcgctt
     7141 gaattagcag aacaaatggg tatcatcttt atagatgaaa tcgacaaagt tgcgacgaat
     7201 aatcataata gtggtcaaga tgtctcaaga caaggtgttc agagagatat tttacctata
     7261 cttgaaggta gcgttattca aaccaaatat ggtactgtga atactgaaca tatgctgttt
     7321 ataggtgctg gagctttcca tgtatctaag ccgagtgact tgataccaga attgcaaggt
     7381 cgttttccga ttagagttga acttgatagt ttatcggtag aagattttgt aagaattttg
     7441 acagaaccaa aattgtcatt aattaaacaa tatgaagcat tgcttcaaac agaagaagtt
     7501 actgtaaact ttaccgatga agcaattact cgcttagctg agattgctta tcaagtaaat
     7561 caagatacag acaacattgg tgcacgtcga cttcatacaa ttttagaaaa gatgttagaa
     7621 gatttatcat tcgaagcacc aagtatgccg aatgcagttg tagatattac cccacaatat
     7681 gttgatgata aattaaaatc aatttcaaca aataaagatt taagtgcatt tattctataa
     7741 aaatatacaa aaggagaaaa attcatgagc ttattatcta aaacgagaga gttaaacacg
     7801 ttacttcaaa aacacaaagg tattgcggtt gattttaaag atgtagcaca aacgattagt
     7861 agcgtaactg taacaaatgt atttattgta tcgcgtcgag gtaaaatttt aggatcgagt
     7921 ctaaatgaat tattaaaaag tcaaagaatt attcaaatgt tggaagaaag acatattcca
     7981 agtgaatata cagaacgatt aatggaagtt aaacaaacag aatcaaatat tgatatcgac
     8041 aatgtattaa cagttttccc acctgaaaac agagaattat tcatagatag tcgtacaact
     8101 atcttcccaa ttttaggtgg aggagaaaga ttaggtacat tagtacttgg tcgagtacat
     8161 gatgatttta atgaaaatga tttggtacta ggtgaatatg ctgctacagt tattggtatg
     8221 gaaatcttac gtgagaagca tagtgaagta gaaaaagaag cgcgcgataa agctgctatt
     8281 acaatggcaa ttaattcatt atcttattct gaaaaagaag cgattgaaca tatctttgaa
     8341 gaacttggcg gtacggaagg cctattaatc gcatcaaaag ttgcagatag agttggtatt
     8401 actagatctg taattgtaaa tgcactacgt aaattagaaa gtgctggtgt aattgaatca
     8461 cgttctttag gaatgaaagg tacttttatt aaagttaaaa aagaaaaatt cttagatgaa
     8521 ttagaaaaaa gtaagtaggt cgatgagtct gggacataat tcaatgtcct agtcgtgacg
     8581 atgttatctt ggcagtagtc aacgaaatga ctttgatgat tatgttgaag tcatttcttt
     8641 atttttgtct aattttcaat caaattgata tttcacatta acttgataga attaccttta
     8701 aatttatgaa aaaatgcttg aaaaacattt gctttagaca cggcattatg ttatatttat
     8761 tttcggctat aaagccaata cacacatatc aaacgtgatt gtgtaaaggg tgcaatattt
     8821 atatattgtc tttacaatag tttgctatgg aggtaattaa ccaataggag gaatttataa
     8881 tggcagtaat ttcaatgaaa caattactag aagcgggtgt tcacttcggt caccaaacac
     8941 gtcgttggaa cccaaaaatg aaaaaatata tcttcactga gagaaatggt atttatatca
     9001 tcgacttaca aaaaacagtg aaaaaagtag acgaggcata caacttcttg aaacaagttt
     9061 cagaagatgg tggacaagtc ttattcgtag gaactaaaaa acaagcacaa gaatcagtta
     9121 aatctgaagc agaacgtgct ggtcaattct acattaacca aagatggtta ggtggattat
     9181 taactaacta taaaacgatc tcaaaacgaa tcaaacgtat ttctgaaatt gaaaaaatgg
     9241 aagaagatgg tttattcgaa gtattaccta aaaaagaagt agtagaactt aaaaaagaat
     9301 acgaccgttt aatcaaattc ttaggcggaa ttcgtgatat gaaatcaatg cctcaagcat
     9361 tattcgtagt tgacccacgt aaagagcgta atgcaattgc tgaagctcgt aaattaaata
     9421 ttcctatcgt aggtatcgtt gacactaact gtgatcctga cgaaattgac tacgttatcc
     9481 cagcaaacga cgatgctatc cgtgcggtta aattattaac tgctaaaatg gcagatgcaa
     9541 tcttagaagg tcaacaaggc gtttctaatg aagaagtagc tgcagaacaa aacatcgatt
     9601 tagatgaaaa agaaaaatca gaagaaacag aagcaactga agaataatca actgttgaat
     9661 ctgacttaga tatagtttaa atgggtgata agatattaat gcttatcacc ttttttaaaa
     9721 agaaaatcga ggcaaattac aaatattcaa ttagagtatt ggcaatcttg cctataataa
     9781 tgctaaaatc ataatatata aaatgataac ttattggagg aataatgaat ggcaactatt
     9841 tcagcaaaac ttgttaaaga attacgtgaa aaaactggcg cgggtatgat ggattgtaaa
     9901 aaagcgctaa ctgaaactga tggtgacatc gataaagcga ttgattacct acgtgaaaaa
     9961 ggtattgcta aagcagctaa aaaagcagac cgtattgcgg ctgaaggttt agtacatgta
    10021 gaaactaaag gtaacgacgc agttatcgtt gaaattaact ctgaaacaga ctttgttgct
    10081 cgtaacgaag gattccaaga gttagttaaa gaaatcgcta atcaagtatt agatacaaaa
    10141 gctgaaactg ttgaagcttt aatggaaaca actttaccaa atggtaaatc agttgatgaa
    10201 agaattaaag aagcaatttc aacaatcggt gaaaaattaa gtgttcgtcg ttttgctatc
    10261 agaactaaaa ctgataacga tgctttcggc gcttacttac acatgggtgg acgcattggt
    10321 gtattaacag ttgttgaagg ttcaactgac gaagaagcag caagagacgt tgctatgcat
    10381 atcgctgcaa tcaaccctaa atatgtttct tctgaacaag ttagcgaaga agaaatcaac
    10441 cacgaaagag aagttttaaa acaacaagca ttaaatgaag gtaaaccaga aaacatcgtt
    10501 gaaaaaatgg tggaaggacg tttacgtaaa tacttacaag aaatttgtgc tgtagatcaa
    10561 gacttcgtta aaaaccctga tgtaacagtt gaagctttct taaaaacaaa aggtggaaaa
    10621 cttgttgact tcgtacgcta tgaagtaggc gaaggtatgg aaaaacgcga agaaaacttt
    10681 gcggatgaag ttaaaggaca aatgaaataa tctgtcataa agtaaaacaa ggaagaagac
    10741 acctttaatg ttgctttatt aaaatgtaaa tcattctaat aaaacgacaa ctgtgtcttc
    10801 tttacttgta tatgttacat atattcacga tagagaggat aagaaaatgg ctcaaatttc
    10861 taaatataaa cgtgtagttt tgaaactaag tggtgaagcg ttagctggag aaaaaggatt
    10921 tggcataaat ccagtaatta ttaaaagtgt tgctgagcaa gtggctgaag ttgctaaaat
    10981 ggactgtgaa atcgcagtaa tcgttggtgg cggaaacatt tggagaggta aaacaggtag
    11041 tgacttaggt atggaccgtg gaactgctga ttacatgggt atgcttgcaa ctgtaatgaa
    11101 tgccttagca ttacaagata gtttagaaca attggattgt gatacacgag tattaacatc
    11161 tattgaaatg aagcaagtgg ctgaacctta tattcgtcgt cgtgcaatta gacacttaga
    11221 aaagaaacgt gtagttattt ttgctgcagg tattggaaac ccatacttct ctacagatac
    11281 tacagcggca ttacgtgctg cagaagttga agcagatgtt attttaatgg gcaaaaataa
    11341 tgtggatggt gtatattctg cagatcctaa agtaaacaaa gatgcggtaa aatatgaaca
    11401 tttaacgcat attcaaatgc ttcaagaagg tttacaagta atggattcaa cagcatcctc
    11461 attctgtatg gataataaca ttccgttaac tgttttctct attatggaag aagggaatat
    11521 taaacgtgct gttatgggtg aaaagatagg tacgttaatt acaaaataaa tttagaggtg
    11581 taaaataatg agtgacatta ttaatgaaac taaatcaaga atgcaaaaat caatcgaaag
    11641 cttatcacgt gaattagcta atatcagtgc aggaagagct aattcaaatt tattaaacgg
    11701 cgtaacagtt gattactatg gtgcaccaac acctgtacaa caattagcaa gcatcaatgt
    11761 tccagaagca cgtttacttg ttatttctcc atacgacaaa acttctgtag ctgacatcga
    11821 aaaagcgata atagcggcta acttaggtgt caacccaaca agtgatggtg aagtgatacg
    11881 tattgcagta cctgccttaa cagaagagcg tagaaaagag cgcgttaaag atgttaagaa
    11941 aattggtgaa gaagctaaag tatctgttcg aaatattcgt cgtgatatga atgatcagtt
    12001 gaaaaaagat gaaaaaaatg gcgacattac tgaagatgag ttgagaagtg gtactgaaga
    12061 tgttcagaaa gcaacagaca attcaataaa agaaattgat caaatgattg ctgataaaga
    12121 aaaagatatt atgtcagtat aaaactaata tacaatgaca tattaaaatg ccagtattaa
    12181 acaataatgt aacatttaaa atgggcatgt ttaattaaat caaagatgca tgtgataatt
    12241 taaattcaca atgagcataa aaatggtgtt taaacaagtt aattaaacat atactttata
    12301 aataataggc attaggtata ttgctataat aaagttatgt aatttttaac ctcagtatgt
    12361 atgtcacatt tctggtgtaa actgtaccga gtcagacttt ggtacagttt ttttatttgc
    12421 ttattcaatg cattaaatga gtatgataaa atgataatga ttgtttagta acttatacta
    12481 tatgacagag atgatcaggc tcggaggaaa gaccatgttt aaaaagctaa taaataaaaa
    12541 gaacactata aataattata atgaagaatt agactcgtct aatatacctg aacatatcgc
    12601 tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta gaattaaagg
    12661 tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg atattggtgt
    12721 taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg aaagtgaagt
    12781 aaattatatt atgaatttgc ctgtcaattt cttaaagaca tttttaccgg aactaattga
    12841 aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat caacgataga
    12901 agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat taatatttgc
    12961 aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt ttgacgagct
    13021 tcatcaacaa ggtttaaata gtgatatcat agatgaaaca tatataaaca atcatttaat
    13081 gacaaaagac tatcctgatc cagagttgtt aattcgtact tcaggagaac aaagaataag
    13141 taatttcttg atttggcaag tttcgtatag tgaatttatc tttaatcaaa aattatggcc
    13201 tgactttgac gaagatgaat taattaaatg tataaaaatt tatcagtcac gtcaaagacg
    13261 ctttggcgga ttgagtgagg agtagtatag tatgaaagtt agaacgctga cagctattat
    13321 tgccttaatc gtattcttgc ctatcttgtt aaaaggcggc cttgtgttaa tgatatttgc
    13381 taatatatta gcattgattg cattaaaaga attgttgaat atgaatatga ttaaatttgt
    13441 ttcagttcct ggtttaatta gtgcagttgg tcttatcatc attatgttgc cacaacatgc
    13501 agggccatgg gtacaagtaa ttcaattaaa aagtttaatt gcaatgagct ttattgtatt
    13561 aagttatact gtcttatcta aaaacagatt tagttttatg gatgctgcat tttgcttaat
    13621 gtctgtggct tatgtaggca ttggttttat gttcttttat gaaacgagat cagaaggatt
    13681 acattacata ttatatgcct ttttaattgt ttggcttaca gatacagggg cttacttatt
    13741 tggtaaaatg atgggtaaac ataagctttg gccagtaata agtccgaata aaacaatcga
    13801 aggattcata ggtggcttgt tctgtagttt gatagtgcct cttgcaatgt tatattttgt
    13861 agatttcaat atgaatgtat ggatattact aggagtgaca ttgattttaa gtttatttgg
    13921 tcaattaggt gatttagtgg aatcaggatt taagcgtcat ttcggcgtta aagactcagg
    13981 tcgaatacta cctggacacg gtggtatttt agaccgattt gacagcttta tgtttgtgtt
    14041 accattatta aatattttat taatacaatc ttaatgctga gaacaaatca ataaacgtaa
    14101 agaggagttg ctgagataat ttaatgaatc tcagaactcc ttttgaaaat tatacgcaat
    14161 attaactttg aaaattagac gttatatttt gtgatttgtc agtatcatat tataatgact
    14221 tatgttacgt atacagcaat catttttaaa ataaaagaaa tttataaaca atcgaggtgt
    14281 agcgagtgag ctatttagtt acaataattg catttattat tgtttttggt gtactagtaa
    14341 ctgttcatga atatggccat atgttttttg cgaaaagagc aggcattatg tgtccagaat
    14401 ttgcgatcgg tatggggcca aaaattttta gttttagaaa aaatgaaaca ctttacacta
    14461 ttaggttatt gcctgttggt ggatatgttc gtatggcagg agatggctta gaagaaccac
    14521 cagtcgagcc tggtatgaac gttaaaatta aactgaatga agaaaatgaa ataacacata
    14581 tcatattaga tgatcatcat aagtttcaac aaattgaagc gatcgaagtt aaaaaatgtg
    14641 attttaaaga tgacttattc atagaaggta tcactgctta tgataacgaa agacatcatt
    14701 ttaaaattgc tagaaagtct ttctttgttg aaaatggtag cttagttcaa attgctccga
    14761 gagacagaca atttgcacat aaaaagccat ggccgaaatt tttaacatta tttgcaggac
    14821 cgttatttaa ctttatatta gctttagtcc tatttattgg tcttgcatat tatcaaggta
    14881 cacctacgtc tactgtagaa caagtcgcag ataagtatcc agctcaacaa gcaggcttac
    14941 aaaaaggcga taaaattgtc caaattggca aatat
//
LOCUS       DAPIDM010000056        14696 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000056 DAPIDM010000000
VERSION     DAPIDM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.056"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            310..1062
                     /locus_tag="RJE42_001474"
     CDS             310..1062
                     /locus_tag="RJE42_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDV5876898.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKLDSKR
                     LYNAKVSFANPTDLENKKTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGEANKTQCSYGGVTFSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEKNVEYDFYNL
                     NGEYGHEVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1265..1534)
                     /locus_tag="RJE42_001475"
     CDS             complement(1265..1534)
                     /locus_tag="RJE42_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876899.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1848..2534
                     /gene="mtnN"
                     /locus_tag="RJE42_001476"
     CDS             1848..2534
                     /gene="mtnN"
                     /locus_tag="RJE42_001476"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV5876900.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQVPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSIEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2554..3081
                     /locus_tag="RJE42_001477"
     CDS             2554..3081
                     /locus_tag="RJE42_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDV5876901.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            3082..4182
                     /gene="yqeH"
                     /locus_tag="RJE42_001478"
     CDS             3082..4182
                     /gene="yqeH"
                     /locus_tag="RJE42_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDV5876902.1"
                     /translation="MSDILKCIGCGASLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4196..5002
                     /gene="aroE"
                     /locus_tag="RJE42_001479"
     CDS             4196..5002
                     /gene="aroE"
                     /locus_tag="RJE42_001479"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV5876903.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDSYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTLARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEETERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            5006..5296
                     /gene="yhbY"
                     /locus_tag="RJE42_001480"
     CDS             5006..5296
                     /gene="yhbY"
                     /locus_tag="RJE42_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV5876904.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKDNKEIELP"
     gene            5299..5868
                     /gene="nadD"
                     /locus_tag="RJE42_001481"
     CDS             5299..5868
                     /gene="nadD"
                     /locus_tag="RJE42_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDV5876905.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKTFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENGMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5858..6442
                     /gene="yqeK"
                     /locus_tag="RJE42_001482"
     CDS             5858..6442
                     /gene="yqeK"
                     /locus_tag="RJE42_001482"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDV5876906.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6443..6796
                     /gene="rsfS"
                     /locus_tag="RJE42_001483"
     CDS             6443..6796
                     /gene="rsfS"
                     /locus_tag="RJE42_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV5876907.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            6799..7515
                     /locus_tag="RJE42_001484"
     CDS             6799..7515
                     /locus_tag="RJE42_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV5876908.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSVNVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLADSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLTQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            7555..8241
                     /locus_tag="RJE42_001485"
     CDS             7555..8241
                     /locus_tag="RJE42_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDV5876909.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRDFENKDTALKQSTSENSSLSKLEDVQVKDGDDSKNKGPVYVDIKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            8333..8794
                     /locus_tag="RJE42_001486"
     CDS             8333..8794
                     /locus_tag="RJE42_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDV5876910.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEDATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            8799..11000
                     /locus_tag="RJE42_001487"
     CDS             8799..11000
                     /locus_tag="RJE42_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDV5876911.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYIAYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNPQFNERVQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVIDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFVFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGLLSNLIFVPYYSIILFPLSILFFITSHFIVGLMPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVINDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGELEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNVKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            11057..12031
                     /gene="holA"
                     /locus_tag="RJE42_001488"
     CDS             11057..12031
                     /gene="holA"
                     /locus_tag="RJE42_001488"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV5876912.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKSPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(12076..12327)
                     /locus_tag="RJE42_001489"
     CDS             complement(12076..12327)
                     /locus_tag="RJE42_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV5876913.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            12673..14496
                     /gene="lepA"
                     /locus_tag="RJE42_001490"
     CDS             12673..14496
                     /gene="lepA"
                     /locus_tag="RJE42_001490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV5876914.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
BASE COUNT         5419 a         1820 c         2671 g         4786 t
ORIGIN      
        1 tttttgttgt gtatcagtat tgtgatgttt taatgcgaat attatatgaa tcattatgaa
       61 atttaataaa aataattgaa ataattatca ttttttctga tatactgtta aacggtttgg
      121 aatttttaag tttacactgt actggttgat ataactcaac taataattgc gaacagagta
      181 tttcaaattg aaaagtatta tgagcgtgat acataatcaa aattgtgggc tcacgaacca
      241 ctacataata aaccatatgt ggttctttat catttatatc tcgctctcaa atgtaaatta
      301 ataattgttt tgggggagtt tgaagttaaa tatttaacag gatttatttt aatattattg
      361 ttagaaggaa tttttacaaa ttcagcaagt gcaatcgaat attcagactt acatcataaa
      421 agtaaacttg attccaagcg tctatataat gctaaggtgt catttgctaa tccaactgat
      481 cttgaaaata aaaaaacaaa cgatagactg ttgaagcatg atttgttatt tcatgacatg
      541 ttcgtaaatg atgattggaa aaaagatttt aaagttgaat tcgaaaatga ggcactttca
      601 aagaaattta taaataagga tatcgatata tttgctggaa attatggata cggatgtcat
      661 gggggagaag ccaataaaac gcaatgtagt tatggtggtg ttacttttag tgacaataat
      721 aagtacgatg attataagaa tataccttgt aatttatgga ttgacggaca tcaaacagaa
      781 atagaactaa ctgcagtaaa aacgaaaaag aaaattgtta ccattcaaga attagatgtt
      841 caattaagaa attatttgaa tgagaagtat aagttgtacg aacaaggtgg cgacattgtt
      901 aaagggtatg ttaaatatca taatgatgat gaaaaaaatg tagaatatga tttttataat
      961 ttaaatggtg agtatggtca tgaggtatta aaaatgtatg ctgataataa aactatcaat
     1021 agtgacaaat tgcatttaga tatctattta ttcaaatcat aatttaagtg agttaatcat
     1081 taaagatctt tgacagtgaa atataaagta cctgtcatta tattgttaca aataaaaaca
     1141 gcagtaagga ggctatcact cagaaaagta cttactgctg ttttatatta gaattatctt
     1201 taatgcacac gggaattcga tacgaaacaa atatgattaa ctgtgtaatt acaaaataaa
     1261 acgactataa taaaaagaag ataaagacga tgataataaa tgcatcgaat ataattttct
     1321 tgatgttttt atcaacaaca tcaggtaagg acattagtat aaatcctaaa atcaaagtat
     1381 agcgacatat gttcataatt gttgctgtaa actcgatatt aaatgaaaac tttaaaaata
     1441 tacttataaa taaaatgaca aacgacgcaa ttgcaataaa actaccgtat gtagctatct
     1501 ttgttacgag actattttga ttattttcag ccattgtaaa atcctcttat ttcatcatta
     1561 ttattttaaa catatcattt tcccgttatt taataaagtt tcttgacacc attggtttat
     1621 gtaattgata tgattagttt ttgaaaaata cacaaattag aagcatcgat ttcactgtta
     1681 tgagttcaaa aataattatt taagatacca agttattgtg atagcttttt ataaaaatat
     1741 agcaaaagtt ttatacagta acgaaaatca tgatacttgt cgttgtagtg attaaaatgt
     1801 gataaaataa caaatgctag gattttttaa aagaggtgaa tcaaaggatg attggtataa
     1861 ttggtgccat ggaagaagaa gtaacaatat taaaaaataa attaacacaa ttaagcgaaa
     1921 tttcagttgc acatgttaaa ttttatactg gcattttaaa agatagagaa gtagtgatta
     1981 cccaaagcgg cattggaaaa gttaatgctg caatttctac gacattacta attaataagt
     2041 ttaaaccgga catcattatt aatacaggtt ctgctggagc tttagatgaa agtttaaatg
     2101 taggtgacgt tcttataagt gatgatgtaa aatatcatga tgcggatgca acagcatttg
     2161 gttatgaata tggtcaagta ccacagatgc cggtagcatt tcaatcaagt aaacctttaa
     2221 tagaaaaagt atctcaagtt gtacaacaac aacaattaac agctaaagta ggcttaattg
     2281 taagtggtga tagctttatc ggtagtattg aacaacgcca aaaaattaaa aaagcatttc
     2341 caaatgcgat ggcggttgaa atggaagcaa ctgcaattgc acaaacatgt tatcaattta
     2401 atgtaccatt tgttgtagtt cgtgcagttt cagacttagc aaatggagaa gcggaaatga
     2461 gcttcgaagc atttttagaa aaagcagctg tatcatcaag tcaaactgtt gaagcattag
     2521 tgtctcaatt ataaaaaggt aaaggtgagt taaatgggtt tagttcgcaa gttttttatg
     2581 ccgaattcat atgttcaatc aatatttcaa attgatttag acaagttagt ggacaaaggc
     2641 gttaaaggta ttattacaga tttagataat acgctagtag gttgggatgt taaagaacct
     2701 acagaacgtg ttaaagcatg gtttaaggaa gctaatgaaa aaggaatcac tattacaatc
     2761 gtgtctaata ataatgagtc tcgtgttgct agttttagtc agcatttaga catcgatttt
     2821 atttttaaag cgagaaagcc aatggggaaa gcgtttgata aagcaataac taagatgaat
     2881 atcagaccag atcaaactgt tgttataggt gaccaaatgc ttactgatgt atttggtggt
     2941 aatcgtcgag gtttatatac aattatggtc gttccagtta aacgaactga tggctttatt
     3001 actaagttta atagattaat tgaaagacga ttattacgtc atttcagtaa aaaaggttat
     3061 atcacatggg aggaaaattg attgtctgac attttaaaat gtatcggttg tggtgcatca
     3121 cttcaatctg aagataaaaa taaacctggt tttgtaccag agcataatat gtttcgtgat
     3181 gacgttattt gcagacgttg tttccgcttg aaaaattata acgaagttca agatgtagga
     3241 ttagaaagtg aagacttttt aaaattatta tcaggacttg cggataaaaa gggcattgtc
     3301 gtcaatgttg tggatgtatt tgactttgaa ggatcattta ttaatgctgt taaacgtatt
     3361 gtcggaaaca aaaaaatcat tttagcagca aataaattgg atttattgcc gaaacaaatt
     3421 aataaacgtc gagttaaaga atggttaaaa cgaacagcaa gaaaatatgg tttggaagct
     3481 gacgatgtcg tattaatttc agctgaaaaa ggctggggca tagacgactt attatcatca
     3541 attgcgaata ttcgagaaaa tgaagatgtg tatattgtag ggacaacgaa tgttgggaaa
     3601 tctacattga ttaataaact gattgaagct agtgttggtg aaaaagatgt agtaacaact
     3661 tcaagattcc ctggaacaac tttagatatg atagatattc cattagatga aacatcattt
     3721 atgtatgata caccaggtat tattcaagat catcaaatga cgcatttagt tagtgaaaaa
     3781 gaattgaaaa ttattatgcc taagaaagaa ataaaacaac gcgtatatca attaaacgag
     3841 gcgcaaacat tattcttcgg cggtctagcg cgcatagatt atgtatcagg tggtaaacgt
     3901 ccgttagttt gtttcttttc taatgacttg aatatacatc gtactaaaac ggagaaggcc
     3961 aatgatttat ggcgtaatca acttggcgat ttattaacgc cacctggaaa tccacaaaat
     4021 tttgatctta atgaggtaaa ggctgttaga cttgaaacag gcaaagagaa acgtgatgtt
     4081 atgatctctg gtctaggctt tataactata ggaccaggag ctaaagtaat cgttcgtgtt
     4141 cctaaaaatg ttgatgttgt attaagaaat tctattttat aaggtgattt aaaaaatgaa
     4201 atttgcagtt atcggaaatc ctatttcaca ttccttgtcg cccgttatgc atagagcaaa
     4261 ttttaattct ttgggattag atgatagtta tgaagcttta aatattccaa ttgaagattt
     4321 tcatttaatt aaagaaatta tttcaaaaaa agaattagat ggctttaata tcacaattcc
     4381 tcataaagag cgtatcatac cgtatttaga tcatgttgat gaacaagcga ttaatgcagg
     4441 tgcagttaat actgttttga taaaagatgg caagtggata gggtataata cagatggtat
     4501 tggttatgta aaaggattgc acagcgttta tccagattta gaaaatgcat acattttaat
     4561 tttgggagca ggtggtgcaa gtaaaggtat tgcttatgaa ttagcaaaat ttgtaaagcc
     4621 caaattaact gttgcgaata gaacgttggc tcgttttgaa tcttggaatt taaatataaa
     4681 tcaaatttca ttggcagatg ctgaaaagta tttagctgaa ttcgatatcg ttattaatac
     4741 aacaccagcg ggtatggctg ggaataacga aagtattatt aatttaaagc atctttctcc
     4801 caatacttta atgagtgata ttgtttatat accgtataaa acacctattt tagaggaaac
     4861 agagcgcaag ggaaaccata tttataatgg cttagatatg tttgtttacc aaggtgcgga
     4921 aagctttaaa atttggacta ataaagatgc tgatattaat tctatgaaaa cagcagtttt
     4981 acaacaatta aaaggagaat aacatatgct tactggcaaa caaaaaagat acttaagaag
     5041 tttagcacac aatattgatc cgatttttca aattggaaaa ggcggtatca acgaaaacat
     5101 gattaaacaa atagatgata cgttagaaaa cagagaattg attaaagtac atgtactaca
     5161 aaataacttt gatgataaaa aagaattagc tgaaacatta agcgaagcta cgcgtagtga
     5221 attagtgcaa gtgattggat ctatgatcgt gatttataga gaatctaaag ataataaaga
     5281 aattgaattg ccataataat gaaaaagata gtactttacg gcggtcagtt taaccctatc
     5341 catactgcac atatgatagt agctagcgaa gtatttcatg aattacagcc agatgaattt
     5401 tattttttac ctagttttat gtctccattg aaaaagcacc atgattttat agacgttcag
     5461 cacagattaa caatgataca gatgattatc gacgagcttg gttttggaga tatttgcgac
     5521 gatgaaatta aacgtggtgg tcaaagttat acctatgaca cgattaagac ttttaaggag
     5581 caacacaaag acagtgagtt gtactttgtc attgggacgg atcagtataa ccaactagag
     5641 aaatggtatc aaattgaata cttaaaagaa atggttactt ttgtagttgt aaatcgagac
     5701 aaaaatagtc aaaatgttga aaatggtatg attgcaattc agatacctag ggtagatata
     5761 agttcgacaa tgattcgaca aagagttagt gaagggaaat ctatccaagt tcttgttcct
     5821 aaatccgttg aaaactatat taagggggaa ggattatatg aacattgaaa aagcaaaacg
     5881 gcttgcaaaa gagaaactgc cagagaaacg ttataatcat tctttaagag ttgcagaaac
     5941 agcgattaaa ttagcagaaa tttatgatgg agacactagt aaagtagaat tagcaggtgt
     6001 attacatgat ttctgtaaat atgatgattt aggtaaaatg tatcaaattg ttcgacaata
     6061 cgaattaggt aatgatctac tgagttatgg tagtgaaata ttgcatggcc cagtgtgtgc
     6121 agcaatcatg gaacatgaat atggtatcaa tgatgaggaa gtattaatgg ctatcaaata
     6181 ccatactacg ggacgtcaac aaatgacaaa aactgaaaaa ctgattttta ttgcagatta
     6241 catcgaacct ggaagaacga ttccaggagt tgatgatatt cgagatatgg catacaatca
     6301 aggtagttta gataagacaa tttatgaaat ttctaaacgt acagtactat ttttaataca
     6361 gaaagatatt acggtatata ataagacgat tgactgttta aattattata actatagtga
     6421 cgaaagaata aaggatgatt aaatgaattc acaagaatta ttagcaattg ctgtggatgc
     6481 aattgacaat aaaaaaggcg aagatacgat ttctttagaa atgaaaggta tcagcgatat
     6541 gacagattat tttgttgtaa cgcacggaaa taatgaacga caagttcaag ctattgctag
     6601 agcggtgaaa gaagtagcca atgaacaaaa tatagaagta aaacgtatgg aaggttacaa
     6661 tgaagcgcgt tggatattaa ttgacttagc tgatgttgtg gtacatgttt tccataaaga
     6721 cgaaagaaat tattataata ttgaaaagtt atatcaagat gcaccattag aatcatatgg
     6781 tcaggttgcg tattaattat gtcgcaatat gcagaaatga gcctagtgta cgatcaattg
     6841 actcaagatc aaccatatga aaaatggttt gaaattgtaa aaaatcactg caaagatgaa
     6901 tcaaatattt tagatattgg atgcggtact ggtagtttaa cagttcaatt agaagcttta
     6961 ggtaatgtta ctggtatgga tttaagtgtt gatatgttga ctgttgcagc tcaaaaatca
     7021 gtaaatgtaa attggcttga aggagatatg acatcgtttg atttgcaaca acaatttgat
     7081 atcatcacta ttttttgtga ctctctaaat tatttgcaag atgaaactgc tgttattgaa
     7141 acattcatca atgtttatca tcatctggct gattcaggtg tatttatttt tgatgtacat
     7201 actgtttata aaatgatgac tttgtttaat aatcaaagtt atattgatga taaaggggac
     7261 atttttttag catgggatgc tgtgcaagga gatttacctt taagtgttta tcatgatatg
     7321 acatttttca ttcgacatga agatgaaaca tattcaagat ttgacgagtc tcattttcag
     7381 agaacatttg atgaaaagac atatttgtct tggttaactc aagttggttt taaacatgtt
     7441 gaaacgttta ctgattttaa tatagatgaa cataatgaag atgcagaaag attgtttttc
     7501 attgcgaaaa aataaaagta aaaccactcg ctcagacgtg tataaagttg aggagtggtt
     7561 ttattgtatc aatttttatt acgctataaa gattttttaa ctcaatggaa gttatatatt
     7621 ataagtgctg ttgttttaat tatggtatta attggtttta tattctggag acaagatgat
     7681 tatacttcaa gagattttga aaataaagat actgctctga aacaaagcac tagtgaaaat
     7741 agtagtttgt ccaaattaga agatgtccag gtcaaagatg gagatgattc caaaaataaa
     7801 ggtcctgtat atgtcgatat aaaaggtgct gttaaacatc ctaatgttta taaaatgaca
     7861 tctaaggata gagtagttga tttacttgat aaagcacaat tattagatga tgcagatgta
     7921 agtcaaatta atttgtctga aaaattaaca gatcaaaaaa tgattttcat tcctcataaa
     7981 ggacaaaaga atgttgaacc acaatttgga gcaaacagtg tgcacgtaaa aaatgggaac
     8041 acaaataata ctaaagtaaa tttaaatacg gcatctgtat cagaattgat gtctgttcct
     8101 ggagtagggc aagctaaagc taatgcaatt gttgaatatc gcaaccaaca aggtgcattt
     8161 caagaaattg acgatttgaa aaaagtaaaa ggttttggaa gtaaaacttt tgataaactg
     8221 aaatcttatt tcacgacata atggattatt ttaattgcaa attttatttt ttgtgattaa
     8281 tcttatatta ttagaaaatg ttactaaatt ttaagtatgg aaggtgaaca tcttggaaag
     8341 aatcaaatgg gaagaatatt ttatggcaca aagtcatttg ctagcattac gttcaacttg
     8401 tcaaagatta tctgtaggtg caacgattgt taaggataat cgtattattg ctggtggcta
     8461 taatggctct gtagctggcg aggtgcattg tatagatgaa ggatgtttaa ttgaagatgg
     8521 acattgtatc agaacgatac atgcagaaat gaatgcttta ttacaatgtg caaaacaagg
     8581 tgtatctact gaagatgcaa caatctatgt tactcatttt ccatgcctaa attgtacaaa
     8641 gtcaattatt caagcaggta taaagcgtat ctactatgca gaagattatc ataaccatga
     8701 gtatgcaact aaattactca aacaatctgg tattgaattt aaaaaaattc cattttcacc
     8761 agaatatgtt gctaaatatc tgactaaagg ttaatgtttt gctgtatgtc gcgttatcaa
     8821 tgattgtagg agtgctttgg aattctagca aagtgctctc tacatttctt ttcattttac
     8881 ttttgtatat tgcttatcgt aaaaataaaa tcgtttatgc ccctatttct ctctttttaa
     8941 tcattttctc ctcatggtat ttacattatt cacaacaagc aatatttaat tatatcaatt
     9001 atattgaacg taatcctcag tttaatgagc gtgttcaagt aatccaaatt caacgtcaag
     9061 gtagtgacac atataaaggt aggttgagtt taaaaaatga aatatatcct ttctttttaa
     9121 cagataaaaa gaattttgat ttaaagaaaa ttgaaagtcg taattgtatt gttaaaggac
     9181 aattcaaagt tattgacaat aagtttgtaa ctcttaaatt acaaagtata gttgtacaaa
     9241 gctgtctaga atcgaaccgg tctaatttaa ttgagaaaca taaacagttt ataatgaatc
     9301 gaatttatga ttcgggtatt aagtttccgg atcgaattat ggcattgatt actggtgacg
     9361 taaaagaaat taatgagcaa tttaaggaac gtgttaaaga gataggtata tatcatttgc
     9421 tggcagttag tggctcgcat atagctgcaa ttgtattctt aatttatcaa cctttaaaac
     9481 gattaaattt acctttattt gtcattaaag gaattacaat cattgtatta gctttatttg
     9541 ctcaatacac aaattatgca ccaagtgctg taagagctat aataatgaca acgcttgtac
     9601 tgcttattac taagcaaatt aaaataaagg gtattcagct attagcattt gtatttataa
     9661 ttatgtttat tttgaatcca ctagttgttt atgatattgg atttcaattt tcattcatca
     9721 tttcattttt tattatgcta ctttttcctt ttttacagca attgtcaaag ttacaatcat
     9781 tattcataat tacgtttatt gcacaattag cttcatttat cgttgccatt ccaaactttc
     9841 atcaacttca atgggtggga ttattatcta atttgatttt tgtaccatac tattcgatta
     9901 tattgtttcc gctatctatt ttattcttta ttacaagtca ttttattgtg ggattaatgc
     9961 cgctaaatta cttggttgac ctaagtttta attttcatga ctggttacta gacctattca
    10021 caagaatcaa gcaatcacat ttttctgttc ccaagtttaa tgattggata tttataatat
    10081 ttataatttc tgtttattac atattttggt tattggctaa acgtaaatat atattggtta
    10141 cgttttggac tataattatt ctgacattat taataacgtt tccaacaaat tcacatcaca
    10201 aaattacaat gttaaatgtg gggcagggag acagtatttt atatgaaggt ggtaagaacc
    10261 aaaatgtctt gattgataca ggtgggaaag tgattaatga tactaaacaa cctagttatt
    10321 caatttctaa atatcatatt ttaccaacgc taaatgaaag agggataaat gaattagagt
    10381 atctaatttt aacacatcca cacaatgacc atattggtga attggaatat attattagtc
    10441 atattaaaat taaacatata gtgatataca ataagggata tagtagtaat acattgatgt
    10501 tattatcgaa attaagccat aagtataacg ttaaacttat ggatgtaagg caagttagta
    10561 gttttaaact tggagatagt agttttctat tttttgatag ttttattcca aatagccgag
    10621 ataaaaatga atattcgatt attactatga ttacatatca aaataaaaaa gttttattaa
    10681 tgggcgatgc tagtaaaaat aatgaatctt tacttctaaa aaaatataac ttgccggaga
    10741 ttgatatttt aaaagtagga catcatggga gcaagacaag tagttctaaa gaatttatag
    10801 agatgattaa gcctaaaata agtttgattt cttctggaaa gaacaatatg tatcatcttc
    10861 ctaacataga agttgttaaa cgattgcaaa ggattcgcag tcgcatttac aatagccaac
    10921 aaaacggtca agttacaata gacttagatg ataatttaaa agttgattca aactcttatg
    10981 gaaatgcaag tggtttatag atacagaaac atgatataat ctattgatgt gttaaacgaa
    11041 tgaaagggtg tactgaatga gcgacaatat tgtagctatt tatggagatg tgcctgaatt
    11101 ggttgaaaaa caaagtgcag aaatcatatc acaatttttg aaaagtgata gagatgactt
    11161 taactttgtg aaatataatt tatacgaaac agagattgca ccaattgttg aagaaacatt
    11221 aacattgcct ttcttttcag ataaaaaagc aattttggtt aaaaatgcat atatatttac
    11281 aggtgaaaaa tcgccaaaag atatggctca taatgtagat caattaatag aatttattga
    11341 aaaatatgat ggcgaaaatt tgattgtctt tgagatatat caaaataaac ttgatgaaag
    11401 aaaaaagtta actaaaactc taaaaaagca tgcaaggctt aaaaaaatag agcaaatgtc
    11461 tgaagaagaa ataaaaaaat ggattcaaag taaattaaat gagaatttca aagatatcaa
    11521 aagagatgca ttagatttat ttattgagtt gacaggtatt aactttaata ttgtctcaca
    11581 agagatagaa aagttgattt tatttttagg cgatagacca acaattaata agcaggatgt
    11641 taaccaaatt atcaatagaa gtttagaaca aaatgtattt ttactgactg aatacattca
    11701 gaaaagaaag aaagaacaag caattcattt agtaaaagat ttaataacaa tgaaagaaga
    11761 acctattaaa ttacttgcac taattacaag taattaccga ttattttatc aatgtaagat
    11821 tctgagtcaa aaaggatata gtggacagca aattgctaaa acaataggcg tacatccata
    11881 cagagtaaaa ttagcgttag gacaagtaag acattatcaa cttgatgaat tattgaatat
    11941 tatagatgct tgtgcggaaa ctgattataa acttaaatca tcatatatgg ataaacagtt
    12001 aatactggaa ttattcattc tatctttata aaaaataaaa gttcaagcat gcgcttgaac
    12061 ttttatttaa aaagattatt tatttgcagt cattaattgt gatttaatac ggtcagcttt
    12121 gtttgaatgt attaaattac tttgagcagc tttgtctact aacttaactg ctaagcttac
    12181 taattcattt ttattatcag cgttatttga aacagctgtt ttagcgtttt taactgctgt
    12241 acgcattgca ctcttttgtg aaatgttgcg tgcttcagct ttttcagttg tttttacacg
    12301 tttaattgca gatttgatat ttgccatttc tgtcacctcc taaaagtgag ttcatcttat
    12361 caaaatttta tttgattaca acaagaaata ttttaacaaa agtctaaccg ttgtgcaatc
    12421 atttatttga tggggttagc ttaaaatttt gcagatttaa taaattgtgt tggaaacaca
    12481 atcaatacgt tttataatta taagtacgta attaacatat taacatatcg caagtatgta
    12541 tttaaataaa gattgttata atttcaaagt tcatccaaga ttatggcgtt tgcatttacc
    12601 tattaaaaac gttattatat caaagatgcg aaagataata cgggtttatt ttatgaaagt
    12661 gagaaggata aaatggataa tgagcaacgc ttaaaaagaa gagagaatat aaggaatttc
    12721 tcgattatag cacatattga ccacggaaaa tctacattgg ctgatagaat tttagaaaat
    12781 accaaatcag ttgaaacaag agatatgcaa gatcagttac tagattcaat ggatttagaa
    12841 agagaacgtg gtattacaat caaattaaac gcagtacgtt taaagtacga agctaaagat
    12901 ggaaatactt atacattcca tttaatcgat acgcctggac acgtcgattt tacatatgaa
    12961 gtgtcacgtt ctttggcagc ttgtgagggc gcgatattag tagtagatgc ggctcaaggt
    13021 atcgaagcac aaacattagc aaatgtttat ttggcattag ataatgagtt agagttattg
    13081 cctgttatta acaaaattga tttacctgct gcagaacctg aacgtgtgaa acaagaaatt
    13141 gaagatatga taggtttaga ccaagacgat gttgttttag caagtgctaa atctaacatt
    13201 ggaattgaag agatactaga gaaaatagtt gaagttgtgc cagctccaga tggtgaccca
    13261 gaagcaccac taaaagcatt aatatttgat tctgagtatg atccatatag aggggtaatt
    13321 tcatcgataa gaattgtaga cggtgttgtt aaagccggag ataaaattcg aatgatggcc
    13381 actggtaaag agttcgaagt aacagaagtt ggaattaata cacctaagca acttccagtt
    13441 gatgaattaa cagttggtga tgttggttat attattgcaa gtattaaaaa tgttgatgat
    13501 tctagggttg gtgacacaat cacattagct agtagacctg catcagaacc attgcaaggt
    13561 tataagaaaa tgaatccaat ggtatattgc ggactgttcc caatagataa caaaaattat
    13621 aatgatttaa gagaagcatt agaaaaatta caattgaatg atgcatcatt agaattcgag
    13681 cctgaatcat cacaagcatt aggttttggt tatagaactg gtttcttagg tatgttacac
    13741 atggaaataa ttcaagaaag aattgaaaga gaatttggca ttgaattaat tgcaacagca
    13801 ccatctgtaa tttatcaatg tattttaagg gacggttcag aagtgacggt tgataaccca
    13861 gcacaaatgc cagatcgtga taaaattgat aaaatatttg agccatatgt tcgtgcaact
    13921 atgatggttc caaatgacta tgtaggtgca gtaatggaat tatgtcaacg taaacgtgga
    13981 caatttataa atatggacta tttagatgat attcgtgtaa atattgttta tgaattacct
    14041 ttagctgaag ttgtatttga tttcttcgat caacttaaat ctaataccaa aggatatgca
    14101 tcatttgatt atgagttcat cgaaaataaa gaaagtaatt tagtgaagat ggatatttta
    14161 ttaaacggtg ataaagtgga tgctctaagc ttcatagttc atagagattt tgcatatgaa
    14221 cgtggtaaag cattagttga aaaacttaaa acgttaattc caagacagca atttgaagta
    14281 cctgtacagg ctgcaatagg acaaaaaatt gtagcgcgta caaatattaa atcaatgggt
    14341 aaaaacgttt tagctaaatg ttatggcggt gacataagcc gtaaacgtaa attacttgaa
    14401 aaacaaaaag caggtaaagc taagatgaaa gcagttggta atgttgaaat tccacaggat
    14461 gctttcttgg ctgtgttgaa aatggatgac gaataagttt ttaaaaaatc tattaacaat
    14521 ttacaatgaa taaagtttaa taactaaaaa gagggagcct aggataaatt aacgtcctgg
    14581 gctttacaat gttatattgg cagccattga cagagttaaa atgagcttat aacaatgggg
    14641 ccccaacata gaagttgacg aaaagtcagc ttacaataat gtgcaagttg gggtgg
//
LOCUS       DAPIDM010000057        14684 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000057 DAPIDM010000000
VERSION     DAPIDM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.057"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(67..1065)
                     /gene="sirC"
                     /locus_tag="RJE42_001491"
     CDS             complement(67..1065)
                     /gene="sirC"
                     /locus_tag="RJE42_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDV5876915.1"
                     /translation="MTEKVNKKDNYHLIFALIFLAIVSVVSMMVGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMVILLVFQTKGHIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTFILGMVIVAVIPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1062..2057)
                     /gene="sirB"
                     /locus_tag="RJE42_001492"
     CDS             complement(1062..2057)
                     /gene="sirB"
                     /locus_tag="RJE42_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDV5876916.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIAFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYLMKKGRQRL"
     gene            complement(2073..3065)
                     /gene="sirA"
                     /locus_tag="RJE42_001493"
     CDS             complement(2073..3065)
                     /gene="sirA"
                     /locus_tag="RJE42_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDV5876917.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDSKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTEGE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3296..4276
                     /gene="sbnA"
                     /locus_tag="RJE42_001494"
     CDS             3296..4276
                     /gene="sbnA"
                     /locus_tag="RJE42_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDV5876918.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4273..5283
                     /gene="sbnB"
                     /locus_tag="RJE42_001495"
     CDS             4273..5283
                     /gene="sbnB"
                     /locus_tag="RJE42_001495"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDV5876919.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5304..7058
                     /gene="sbnC"
                     /locus_tag="RJE42_001496"
     CDS             5304..7058
                     /gene="sbnC"
                     /locus_tag="RJE42_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDV5876920.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSEITKENGQTI
                     LIYKRETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQKIVKMNRDLS
                     REWEVARVEEGLTTAATQLTKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7051..8294
                     /gene="sbnD"
                     /locus_tag="RJE42_001497"
                     /pseudo
     CDS             7051..8294
                     /gene="sbnD"
                     /locus_tag="RJE42_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
     gene            8284..10020
                     /gene="sbnE"
                     /locus_tag="RJE42_001498"
     CDS             8284..10020
                     /gene="sbnE"
                     /locus_tag="RJE42_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDV5876921.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIVSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPTSKLSQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNSLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWKLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            9929..11779
                     /gene="sbnF"
                     /locus_tag="RJE42_001499"
     CDS             9929..11779
                     /gene="sbnF"
                     /locus_tag="RJE42_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDV5876922.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TIDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVK
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEIIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGITDATTISET"
     gene            11754..12530
                     /gene="sbnG"
                     /locus_tag="RJE42_001500"
     CDS             11754..12530
                     /gene="sbnG"
                     /locus_tag="RJE42_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDV5876923.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDED"
     gene            12530..13732
                     /gene="sbnH"
                     /locus_tag="RJE42_001501"
     CDS             12530..13732
                     /gene="sbnH"
                     /locus_tag="RJE42_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDV5876924.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVEKFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIEAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13736..14500
                     /gene="sbnI"
                     /locus_tag="RJE42_001502"
     CDS             13736..14500
                     /gene="sbnI"
                     /locus_tag="RJE42_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDV5876925.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4883 a         2409 c         2928 g         4464 t
ORIGIN      
        1 caacggttag ttagcttaaa cattttgcta acgttgttgt gaggtcattt tgttttaata
       61 atatcgttat aactttttca cggttaataa taagtatatg aagaatgggg caccaaaagc
      121 agcaataaat acacctgctg gcacttcttt aggtaagaat aaggtacgcc caattaagtc
      181 tgcaataaca attgatatgg caccaatcat tgctgacatt agtaactttt tagcataact
      241 tccgcgaacg attgttttcg cgatgtgtgg tgcaattaaa ccgacaaacc caatatttcc
      301 tactaaactg attgccatag atacgagtat tgtagaagtg attaattgaa tcagcttcat
      361 acgttgtaca tgtaaaccta agccaatcgc tacagggtca tcaagtatag atattttcat
      421 tttaggtata acaagaaata acaacggaat aacagctacg attaccatac ccaaaatgaa
      481 tgtatcttta aacgttgcac cgtaaagact tccgactaac caagtataag ctttggctgc
      541 agatagttgc tttgttgtaa taagtagccc ttgtacaagt gcaataaata atgtttgcat
      601 tgaaataccg atgattatga gtgttgtcgg tcgaatgtga cctttcgttt gaaacactaa
      661 tagaatcacc attgcaattg cgccacctaa tacagcaaat agaggaagta aatgtatcgt
      721 taaatggctg aaaaatgcaa taaagacaac agcacttaag ctagcaccac ctgtgattcc
      781 gataatatct ggtgaggcaa ttggattttt caagacattt tgtaacatta aaccgctcat
      841 ccctagtgct gcaccagcta atatcgcaag tgtaatgcga ggtaagcgta atacttctaa
      901 agtgaattga tctatactgt catttggatt tataaagtac atcagtacgc gttgtaatgg
      961 tataaagctt gaaccaacca tcatacttac cactgaaacg atggctaaaa agattaacgc
     1021 gaagatgaga tggtaattgt cctttttatt aaccttttcg gtcataagcg ttgacgtcct
     1081 ttcttcataa gatagattaa gacaatagcg ccaatgacag cggtaacgac accgataggc
     1141 aactctagtg gcttaataat aatacgagca acaatgtctg agataatcat taggattgct
     1201 ccagctaatg cagtaaaagg aattaaatac ttatagtttg gtggtaataa tcgtttgcta
     1261 atattcggta cgataagacc cacaaaggcg attgatccag ctactgctac cgaaataccg
     1321 gctaacatac tgatgagcat aataatcatc catttgatta attttatgtt ttgaccgagg
     1381 ccggttgcaa tgtcgtcact tgtcatcaag atgttgatgt gtgcagccat gctaaatgca
     1441 attaaaataa gtatcaatac aagcggaata atccatggga tatcccaaat gttacgtaat
     1501 gaaacggagc cactcaacca aaataatagg ccttgtaagt ctgtttcatt cataatgaga
     1561 atgccttgag taaaagctgt aaataacatc gcaatcgcag cacctgccaa aatgacacgg
     1621 tgaggtgaaa atagggtttg tctaaacata cctagtgcaa cgactaagac tgtaacaaca
     1681 atagctccca aaaacgcgat gacaacaatc attttaaaag attgaatttg gataaatgta
     1741 atactaaaga tgacaaaaaa tactgcgcct gcattgacac cgaaaagccc tggtgatgct
     1801 attgggtttc gtgtaagtgc ttgcatcaac aagcctgaga cagcaagggc agcaccagtc
     1861 aataacgcaa tgatggttct cgacgcccgt gcaccagtga caacatcatg taaatcgttt
     1921 tcactatcaa agttgaataa cgcctgtatc accgtacctg gtgacacaag cgtatttcca
     1981 atcattaaac ttaagatagc tactattgca agacataaac cagcaataac gatttggtat
     2041 tttggtttaa gtagcatcgt aaaactcctt aattattttg attgtttttc aatatttaac
     2101 ttttcatata aatcgtcaat aagttttaat gatgatttat atccgccagc taaattccaa
     2161 gtgatttcat ctaaatcatc agatacttgg ttgtttttaa ctgcgtctaa atttttccac
     2221 tctttacttg aagtccattc gccttcagtc tttttaacta atgcagcatc tttcgcattt
     2281 ggatctgatt ttactacaaa aatatgatca gcgttcatta atggaatgct ttctttagat
     2341 gtaagttgga taatatcttt accattatca acttgttttt gtaagtcttt attacgtttg
     2401 aatcctaaat catttaagat ttcaccagca tatccaccag cataaattct tgtatgatca
     2461 gcacggaagt taacaactga agctttcaat ggccatgcat ctttatactt tgcttttgca
     2521 tctttttgga atgcagctac tttatcatcg tactttttaa gtaaatcttc agcttctttt
     2581 tctttcccta aagctttccc cattaactta gttgtatctt tgaatttgaa aactgtatca
     2641 gtagaaactg ttggtgcgat tttagataat tgatcgtaaa ctttttcatt tctaactttt
     2701 gacgcgacaa ttaagtccgg ttttaattta gagatttcct ctaagttagg tgcaggttct
     2761 tgacctacaa tcttagtatc ttttaaatca ttttttatgt attcgaattt cggtttttgt
     2821 gtccatgatt ctacagcacc tacaggttta acacctaaag atacagcgac gtcagtggca
     2881 ccttgatata gcgtaacaac acgctttggt ttccctttaa tttcagttgt acccattgca
     2941 tgtttaattg aagttgtttc cttatctttg ctatcagatg attgcttatt tgaattccca
     3001 ctacatcctg ctaaaacaag taggaaagca agcgtaacaa caagcatttt aattacttta
     3061 ttcattgact aattagcctc cttcgtgatg tatgacaatg agaatcatta tcacggttta
     3121 gtatgaatta aattttttcc taagtcaata aaatatttat gatttacatg caacttataa
     3181 ttatttgaca tataaatgca taaaaaatat aatcctaatt acttgatagt gagaatcatt
     3241 atcaattagg taacacacaa tattatagaa ttttaaattt gaggaggaag cgcttttgat
     3301 tgaaaaaagt caagcatgtc acgattcatt gttagattct gtagggcaaa cacctatggt
     3361 tcaacttcat caactatttc cgaaacatga agtgtttgca aagttagagt atatgaatcc
     3421 tggaggcagc atgaaagatc gacctgccaa gtacatcatt gaacatggta tcaaacatgg
     3481 tttaatcact gagaatacac atttaattga aagtacttct ggaaatttag gcattgcgtt
     3541 ggcaatgata gctaaaataa agggattaaa actcacgtgt gttgttgatc ctaaaatatc
     3601 accaacaaat ttgaaaatca ttaaaagtta tggtgccaat gtagaaatgg ttgaagaacc
     3661 tgatgcacat gggggttatt taatgactcg tattgcaaag gtgcaagaac tgttagccac
     3721 tattgacgat gcatattgga ttaatcaata tgcaaatgag ttaaattggc aatcccatta
     3781 tcatggtgca ggcacagaga ttgttgaaac aattaagcaa cctatagatt attttgtcgc
     3841 gccagtcagc acgacaggta gcattatggg tatgagtaga aaaattaaag aagtgcatcc
     3901 aaacgcacaa attgttgctg ttgatgcgaa agggtcagtc atttttggtg acaaacctat
     3961 taatagagaa ttacctggta tcggtgctag tcgtgtaccc gaaatattga atagatcaga
     4021 aattaatcaa gtgatccatg tagatgatta tcaatctgct ttgggctgtc gaaaactgat
     4081 tgattatgaa ggcatatttg ccggaggttc aacaggttcg attattgcag cgattgagca
     4141 gttaataacg tcaattgaag aaggtgcaac aattgtcacg attttaccag atcgaggcga
     4201 tcgttactta gatttagttt attcagatac atggttagaa aaaatgaaat caagacaagg
     4261 agttaaatca gaatgaatag agagatgttg tatttaaata gatcagatat tgaacaagcg
     4321 ggaggaaatc attcacaagt ttatgtggac gcattaacag aagcattaac agcgcatgcg
     4381 cacaatgatt ttgtacaacc gcttaagccg tatttaagac aggatcctga aaatggacac
     4441 atcgcagatc gaattattgc aatgccaagt catatcggtg gtgaacacgc aatttcaggt
     4501 attaagtgga taggtagtaa gcacgacaat ccatcgaaac gtaatatgga gcgtgcaagt
     4561 ggtgtcatta ttttgaatga tccagaaacg aattatccaa ttgcagttat ggaagcaagt
     4621 ttaattagta gtatgcgtac tgcagcagtt tcagtgattg cagctaagca tttggctaaa
     4681 aaaggattta aagacttaac aatcattggt tgcgggctaa tcggagacaa gcaattacaa
     4741 agtatgttag agcaattcga tcatattgaa cgcgtgtttg tttacgatca attctctgaa
     4801 gcgtgtgcac gctttgttga tagatggcaa caacagcgtc cggaaattaa ttttattgcg
     4861 acagaaaatg ctaaagaagc agtatcaaat ggtgaagtag tcattacatg taccgtaacg
     4921 gatcaaccat atattgaata tgattggtta caaaagggtg catttattag caacatttct
     4981 atcatggatg tgcataaaga agtctttatt aaagctgaca aagtcgtagt agatgactgg
     5041 tcacaatgta atcgagaaaa gaaaactatt aaccaattgg tgttagaagg taaattcagc
     5101 aaagaagcac ttcatgctga acttgggcaa cttgtgacag gtgacatacc aggacgtgaa
     5161 gacgatgatg aaatcatatt acttaatccg atgggtatgg ctatcgaaga tatttcaagt
     5221 gcttacttta tttatcaaca ggcacaacaa caaaatattg ggacaacatt gaacctatat
     5281 taagaatgcg aggtgtctga atattgcaga atcatacagc agtcaataca gcacaaacga
     5341 taatattaag ggatttagtt gatgcattat tatttgaaga tatagccggt attgtatcga
     5401 atagtgagat tactaaagaa aatggacaaa ccattttgat atacaaacgt gaaacacaac
     5461 aaataaagat acctgtttat tttagtgctt taaatatgtt tcgttatgaa agttcacaac
     5521 caattacgat agagggaagg gcgtctaagc aacctttaac ggcagctgaa ttttggcaaa
     5581 aaatcgtcaa aatgaatcgt gatttaagtc gtgaatggga agtggctcgc gttgaagaag
     5641 gactgactac tgctgccaca cagcttacta aacaattatc agaattagat ttagcgtcac
     5701 atccttttgt gatgtcagag cagtttgcaa gtttaaaaga tcgtccattt catccattag
     5761 ctaaagaaaa aagaggatta agagaagcgg attatcaagt gtatcaagct gaattaaatc
     5821 aatcatttcc tttaatggtt gcagcagtta aaaagacaca tatgattcat ggcgatactg
     5881 caaatattga tgaattagaa aatttgacag cacctataaa agaacaagcg acagatatgt
     5941 taaatgatca agggttatca atagatgact atgtactatt tccggtacat ccttggcaat
     6001 atcaacatat tctgccgaac gtctttgcga aagagattag tgaaaaattg gttgtactat
     6061 taccgttaaa atttggagat tatctatcgt cttcaagtat gcgttcatta attgatattg
     6121 gcgcaccgta taaccatgtc aaagtaccat ttgcaatgca gtcattaggt gcattaaggc
     6181 taacgcctac gcgttacatg aaaaacggag aacaagcaga acaattatta cgtcagctta
     6241 tagaaaaaga tgaagcacta gctaagtatg tcatggtttg tgatgaaaca gcttggtggt
     6301 catatatggg tcaagataat gatattttca aagatcaatt aggtcattta actgttcagc
     6361 taagaaaata tcccgaagtg ctagccaaaa atgacacgca acagctagtg tcaatggcag
     6421 cactcgcggc aaatgatcgc accttatatc aaatgatttg tggaaaagat aatatttcta
     6481 aaaatgatgt catgacgtta tttgaagata tcgcacaagt ctttttaaag gtaacactat
     6541 catttatgca atacggcgca ttaccagagt tgcatggtca aaatatattg ttgtcatttg
     6601 aagatggacg tgtacaaaaa tgcgtgttac gtgatcatga tactgtcaga atttataaac
     6661 catggctaac agcacatcag ctttcattgc cgaagtatgt cgtcagagaa gatacaccta
     6721 atacgctaat taatgaagat ttggaaacat tctttgctta ttttcaaaca ttagctgtat
     6781 cggtaaatct atatgccatt attgatgcaa ttcaagattt atttggtgta agtgagcatg
     6841 aacttatgtc gttgttaaaa caaattttaa aaaatgaagt ggcaactatt tcctgggtta
     6901 caactgatca gctagctgtc agacatattt tatttgataa acagacgtgg ccatttaaac
     6961 aaattttatt accattgcta tatcaacgtg atagtggtgg aggtagtatg ccttcaggtt
     7021 taactaccgt accaaatcca atggtgacat atgattaatc agtctatatg gcgcagtaac
     7081 tttcgcattt tatggctcag tcagtttata gcgattgctg gactgacagt acttgtgcca
     7141 ttattgccaa tttatatggc atcactacaa aatctatcag tcgtagaaat acagttgtgg
     7201 agtggtatag cgattgctgc tccagctgta acgacgatga tagcttcgcc gatatggggg
     7261 aagctaggtg ataagatcag tcgaaaatgg atggtgttaa gagcgttact tggtttggcg
     7321 gtatgcttat ttttaatggc attgtgtact acaccattac agtttgtact tgtgaggtta
     7381 ttgcagggac tatttggtgg tgttgtcgat gcatcaagtg cgtttgcgag tgcagaggcg
     7441 ccagctgaag atcgtggaaa ggtattagga aaactgcaaa gttcagtcag cgggggccat
     7501 taattggcgg tgttacagct tcgatattag gttttagtgc gttactgatg agtattgccg
     7561 ttattacttt tattgtctgt attttcggtg cattaaaatt gattgaaacg acacatatgc
     7621 gaaaatcaca aacaccaaat attaataaag gtattcgccg ctcatttcaa tgtctattat
     7681 gcacacaaca aacatgtcga tttattatcg ttggcgtttt agcaaacttt gctatgtatg
     7741 gcatgctaac tgcattatca ccacttgctt catcagtgaa tcatacagcg atagatgacc
     7801 gtagtgtgat tggattttta cagtccgcat tttggacggc ttcaatatta agcgcgcctt
     7861 tatggggtcg ctttaatgat aaatcatatg ttaaatcagt atatatattt gccacgattg
     7921 catgtggttg tagtgcgata ctgcaaggtt tagcgacgaa tatagagttt ttaatggctt
     7981 caagaatact tcaaggatta acatatagtg cattgattca aagtgtcatg tttgttgttg
     8041 taaatgcttg tcatcagcaa cttaaaggca catttgttgg aacgacgaac agtatgttag
     8101 ttgttggtca aattattggc agtcttagtg gcgctgccat tactagttat actacaccag
     8161 ctactacgtt tatcgttatg ggcatagtat ttgctgtaag tagtttattt ttaatttgtt
     8221 caaccatcac taatcaaatc aacgatcaca cattaatgaa attatgggag ttgaaacaaa
     8281 aaagtgcaaa ataaagaatt aatacaacat gcagcgtatg cggctatcga acgtatttta
     8341 aatgaatatt ttagagaaga aaatttatat caagtaccac ctcaaaatca tcaatggtct
     8401 atacaattat cagagctcga aactttaacg ggtgaatttc gctattggtc tgcgatgggg
     8461 catcatatgt atcatccaga ggtatggctt attgatggaa aaagtaaaaa aataacaact
     8521 tataaagaag caattgcgcg tattttgcaa catatggctc aaagtgcaga taatcaaacg
     8581 gcagtgcaac aacatatggc gcaaattgtg tctgacatcg ataatagcat tcatcgcacg
     8641 gcgcgttatt tgcaaagtaa cacaatagac tacgtagagg atcgttatat cgtttcagaa
     8701 caatctttat acttaggtca tccatttcat ccgactccta agagtgcaag tgggttttca
     8761 gaagcagatt tagagaaata tgcacccgaa tgtcatacat cattccaatt gcattattta
     8821 gctgtgcatc aagatgttct gctcacgcgc tatgtagaag gtaaagaaga tcaggttgag
     8881 aaagtgttgt atcaattagc agacatagat atatcagaga tacccaaaga ttttatttta
     8941 ttaccaacac atccttatca aatcaatgtg ttgcgacagc atccacagta tatgcaatat
     9001 agtgaacaag gtttaataaa agaccttggc gtttccggtg attcagtgta cccgacgtct
     9061 tcggttagaa ctgtattttc aaaagcatta aacatttatt taaaattacc gatacacgtt
     9121 aaaatcacta attttatacg tacgaatgac cttgaacaga ttgaacggac aattgatgcc
     9181 gcgcaagtta tcgcatcagt caaagatgag gttgaaacac cccattttaa attgatgttt
     9241 gaagaaggat atcgtgcatt gttaccgaat ccattaggac aaacagttga acctgaaatg
     9301 gatttattaa ctaatagcgc catgattgtt cgtgaaggga taccaaatta ccatgctgat
     9361 aaagatattc atgtattggc gtcattattt gaaacgatgc ctgattcacc gacctctaag
     9421 ttatcacaag tgattgagca aagtggttta gcaccagaag catggcttga atgttatttg
     9481 gatcgtacct tattgccgat attaaagctg tttagtaaca caggcattag tctagaagca
     9541 catgtacaaa attcattaat tgaattaaaa gatggcatac ccgacgtatg ctttgtcaga
     9601 gatcttgaag gcatttgtct atctagaacg attgctactg aaaagcaact tgtgccaaat
     9661 gttgttgcag catcaagccc tgttgtatat gcacatgatg aagcatggca tcgtcttaaa
     9721 tattacgttg tagtaaatca cttaggacat ttagtatcaa ctattggtaa agcgactaga
     9781 aatgaagttg tgttatggaa acttgtagcg catcgtctta tgacttggaa aaaagaatac
     9841 gcgaataacg cagtatttgt tgactgtgta gaagatttat atcaaacgcc gaccattgcg
     9901 gctaaagcga atttgatgag taaattgaat gattgtggtg caaaccctat ttatacacat
     9961 ataccaaatc caatttgtca taacaaggag gtatcgtatt gtgaatcaaa caattcttaa
    10021 tcgtgtaaag actagagtga tgcaccaact ggtatcatcg cttatttatg agaatattgt
    10081 tgtgtataaa gcgtcatatc aagacggtgt cggtcatttt acaatagaag gacatgattc
    10141 agagtatcgt tttactgctg aaaagacaca tagctttgat cgtatacgta tcacatcacc
    10201 aattgagcgt gtcgtaggag atgaggcaga tacaacaata gactatacac aattactgag
    10261 agaggttgta tttacatttc ctaaaaatga tgaaaagcta gaacaattta ttgtcgagtt
    10321 attacagaca gaactaaaag atacgcaaag tatgcagtat cgagaatcaa acccaccagc
    10381 aacacctgag acatttaacg actatgaatt ttatgcgatg gaagggcatc agtatcatcc
    10441 aagttacaaa tcacgtttag gatttacatt gagtgataat ttgaaatttg gtcctgactt
    10501 tgtaccaaac gttaaactgc agtggttagc tatcgacaaa gataaagtag aaacgacggt
    10561 atcaagaaat gttgtagtta aagaaatgtt acgtcaacaa gttggcgata agacttatga
    10621 acattttgta cagcaaattg aagcgtctgg caaacatgta aatgatgttg agataatacc
    10681 tgtacaccca tggcagtttg aacatgtcat ccaagttgat ttggctgaag aaaggcttaa
    10741 tggcacagta ctatggttag gggaaagtga tgagctatat caccctcaac aatcgattcg
    10801 tacgatgtcg ccaatagaca cgacaaaata ttatttaaag gtaccaataa gtataacgaa
    10861 tacttcaacg aaacgagtgt tggcgcctca tacaattgaa aatgcagcgc aaattacgga
    10921 ttggttaaag cagatacagc aacaagatac gtatttaaaa gatgaattaa agacagcttt
    10981 tctaggggaa gtcttaggac agtcttattt aaatacacaa ctttcgcctt ataaacaaac
    11041 tcaagtttat ggtgcgttag gtgttatatg gcgtgaaaat atatatcata tgttaatcga
    11101 tgaagaggat gcgataccat ttaatgcact ttatgcaagt gataaggatg gtgtaccatt
    11161 cattgaaaat tggattaaac aatatggttc tgaagcttgg acaaagcaat ttttagctgt
    11221 agcgattcgt ccaatgattc atatgcttta ttatcacggt attgcctttg aatcgcatgc
    11281 acaaaatatg atgctcattc atgaaaatgg ttggcctaca cgtattgcct taaaagattt
    11341 ccatgatggt gttcgtttta agcgtgagca tttaagtgaa gctgcttcac acctgacatt
    11401 aaagccaatg ccagaagcac ataaaaaagt gaatagtaat tcatttattg aaacagatga
    11461 cgaacgttta gtacgcgact ttttacatga tgcatttttc tttattaata tcgccgaaat
    11521 catcttattt attgaaaagc aatatggtat cgatgagcag cggcaatggc aatgggttaa
    11581 aggcatcatc gaggcgtatc aagaagcatt tccagagttg aataactatc aacatttcga
    11641 tttgtttgaa cctacgattc aagttgaaaa gttaacgaca cgtcgattat taagtgactc
    11701 cgagttaaga attcatcatg ttacaaatcc attaggtgta ggaggtatta ctgatgcaac
    11761 aactatctct gaaacataga ttaaacaatg gtgattcagt ttatggcatt tttaattcta
    11821 taccggaccc attgatgata gaggttatcg cagcaagcgg gtatgacttt gttgtgattg
    11881 atacagaaca cgtggcgatt aatgatgaga cactagcgca tttaattcgt gcagctgaag
    11941 cagcgcatat tataccaatt gtacgtgtca ctgcagtaat agatagagat atcattaaag
    12001 tgttggatat gggtgcgaga ggtattattg tgccacacgt taaagatcgt gagacagttg
    12061 agcatattgt gaaattaagt cgttattacc cgcaaggatt aagaagtttg aatggtggtc
    12121 gcatggcaag atttggacgt acaccattac ttgatgcaat ggagatggct aatgagcata
    12181 ttatggtgat tgccatgata gaagatgttg aaggggttat ggccattgac gatatagcac
    12241 aagtcgaagg tttagacatg atagtcgaag gtgccgcaga tttatcccag tcacttggca
    12301 taccatggca aacgcgtgat gatcaagtaa catcacatgt tcaacatatt tttgaagttg
    12361 tgaatgctca tggtaaacat ttttgtgcat taccacgtga agatgaagat attgcaaaat
    12421 ggcaggcaca aggtgtacaa acatttattt taggtgatga tcgtggaaaa atatatcgcc
    12481 atttaagtgc atctctagcg acgtctaaac agaaggggga tgaagactaa tgcgtatagt
    12541 tcaacccgtt attgaacaat taaaagcaca atctcatcca gtttgtcatt atatctatga
    12601 tttagtcgga ctggaacatc atttgcaaca tattacatcg tcattgccta gtaattgtca
    12661 gatgtactat gcaatgaaag caaatagtga acgaacaatc ctagatacaa ttagtcagta
    12721 tgttgaagga tttgaagttg catctcaagg tgaaatagca aaaggtcttg cttttaaacc
    12781 agcaaatcat attatttttg gtggccctgg taagacagac gaggaactaa gatatgcagt
    12841 aagtgaaggt gttcagcgta ttcatgttga aagtatgcat gaattacaac ggctaaatgc
    12901 catcttagaa gatgaagata agacacaaca cattttattg cgtgttaatt tagcaggacc
    12961 atttcccaat gcaacgttgc atatggcagg acgcccaaca caatttggta tttctgaaga
    13021 cgaagttgat gatgtcattg aagctgcgct agcaatgcca aatattcatc tagatggatt
    13081 tcattttcat tctatttcta acaatttaga ctcgaattta catgtcgatg tagtgaaact
    13141 ctattttaaa aaagcaaaat catggtctga aaaacatcga tttccactca aacatatcaa
    13201 tcttggtggt ggtatagggg ttaactatgc agatttaact agccaatttg agtgggataa
    13261 ttttgtagaa aaatttaaaa cactgatcgt tgagcaagaa atggaagatg tgacattgaa
    13321 ctttgaatgt ggtcgcttta ttgtggcaca tattggttac tatgtgacag aagtgctaga
    13381 tattaagaaa gtgcatggtg cttggtatgc cattttaaga ggaggtacgc aacaatttag
    13441 actgccggta tcttggcagc ataaccatcc ttttgacatt tatcgctata aggacaatcc
    13501 atattcattt gaaaaagttt caatttcgag acaggacaca acgttagtcg gtcaattatg
    13561 tacaccgaaa gatgtctttg ctagagaagt acagatagag gcaatcagta caggcgacgt
    13621 tattgttttc aaatatgcag gtgcatacgg atggtctatt tcacatcacg atttcttaag
    13681 ccatccacat cctgaattta tttatttaac gcaaacaaag gaggatgaat aactattgaa
    13741 tcatattcat gaacatttaa aattggtacc agtagataag attgatcttc acgaaacatt
    13801 cgaaccttta agattggaaa aaacgaaaag tagtattgaa gcagatgatt ttatacgtca
    13861 tcctatttta gtgacagcga tgcaacatgg tagatatatg gttatagatg gtgtgcatcg
    13921 gtatacaagt ttgaaagcgt taggatgtaa gaaagttcca gtgcaagaaa tccatgaaac
    13981 acaatattca attagtacat ggcaacataa agttccattt ggtgtgtggt gggaaacgtt
    14041 acaacaagaa catcgcttac catggactac tgagacaaga caagaagcgc catttattac
    14101 gatgtgtcat ggtgatacag aacaatattt gtatacaaaa gatttaggcg aagcacattt
    14161 tcaagtatgg gaaaaggttg tcgcaagtta tagtggttgt tgttctgtag agagaattgc
    14221 acaaggtaca tatccttgtc tttctcaaca agatgtactc atgaagtatc agccattgag
    14281 ttataaggaa attgaagcgg ttgttcataa aggggaaact gtgccagcag gtgtgacacg
    14341 ctttaatatt tcaggacgat gtcttaatct tcaagtacca ctggcattac ttaaacaaga
    14401 tgatgatgtt gaacaactgc gcaattggaa gcagttttta gcagataagt ttgccaatat
    14461 gagatgctat actgaaaaag tatacttggt ggagcaatag ttttactgtg atgttgaggg
    14521 aaatatgatg atttagccta ttgatagcga aaatataata aaacaatata gtgtggagaa
    14581 cttttgatat tttataaata ttgaagttct ccatttttgt attttgcata taaaaattaa
    14641 atgaaataag gtatattaag gtaaagtata aattttaaat aaat
//
LOCUS       DAPIDM010000058        14679 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000058 DAPIDM010000000
VERSION     DAPIDM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.058"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..695
                     /locus_tag="RJE42_001503"
     CDS             <1..695
                     /locus_tag="RJE42_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5876926.1"
                     /translation="DSKETQIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFEKGDKGT
                     WVISSAMVKQPKGKIMRSRGMYLFLNRNTRTAKGYFIVDVTSKDTLKKTEDKEKRYPV
                     KMVNNKIIPIGPINDEGIKKEIENFKFFSQYGDFKGLKNYQNGDYSYNSEAPSYSAKF
                     QLSNDDYNVKQLRKMYDIQTKKAPKLLLKGTGDLKGSSIGHKDIEFTFVENQEENVFF
                     TDSLEFTPSEDY"
     gene            723..1502
                     /locus_tag="RJE42_001504"
     CDS             723..1502
                     /locus_tag="RJE42_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5876927.1"
                     /translation="MNNFRQCALCIGTSVLILLVSGCSGVFDAPEGSKEIQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVVRSEMIIQPKGKSLTSRGMTLYMNR
                     NTRTTTGYFSIEEIDSRKSLDERETEKKYPVKMKNNKIIPTEEIKDEKLKKEIENFKF
                     FVQYGSFKGIENYENGDISYNSEAPIYSAKYKLKNDDYNVKELRKRYNIPTEKAPKLL
                     LKGSGDLKGSSVGYKEIEFIFLENKKENIYFSDGLNLIPSD"
     gene            1563..2363
                     /locus_tag="RJE42_001505"
                     /pseudo
     CDS             1563..2363
                     /locus_tag="RJE42_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2382..3177
                     /locus_tag="RJE42_001506"
                     /pseudo
     CDS             2382..3177
                     /locus_tag="RJE42_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            3346..4158
                     /locus_tag="RJE42_001507"
     CDS             3346..4158
                     /locus_tag="RJE42_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5876928.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKADELSNE
                     GMVLYLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDER"
     gene            4148..5473
                     /locus_tag="RJE42_001508"
     CDS             4148..5473
                     /locus_tag="RJE42_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876929.1"
                     /translation="MKDKYKIDPGPIKYNTEETTAISKISYEIENAKLYGIKSRTIGR
                     QIDQLKEAEKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKL
                     KEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVKLSINEIVDKDQVLASQIGGLL"
     gene            5470..5793
                     /locus_tag="RJE42_001509"
     CDS             5470..5793
                     /locus_tag="RJE42_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876930.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            5812..6126
                     /locus_tag="RJE42_001510"
     CDS             5812..6126
                     /locus_tag="RJE42_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876931.1"
                     /translation="MTLIEPDMNLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(6449..6709)
                     /locus_tag="RJE42_001511"
     CDS             complement(6449..6709)
                     /locus_tag="RJE42_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876932.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYIIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            6841..8043
                     /locus_tag="RJE42_001512"
     CDS             6841..8043
                     /locus_tag="RJE42_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV5876933.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEGAITKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(8702..8764)
                     /locus_tag="RJE42_001513"
     CDS             complement(8702..8764)
                     /locus_tag="RJE42_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDV5876934.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(8828..8896)
                     /locus_tag="RJE42_001514"
     CDS             complement(8828..8896)
                     /locus_tag="RJE42_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDV5876935.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(8948..9013)
                     /locus_tag="RJE42_001515"
     CDS             complement(8948..9013)
                     /locus_tag="RJE42_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDV5876936.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(9045..9110)
                     /locus_tag="RJE42_001516"
     CDS             complement(9045..9110)
                     /locus_tag="RJE42_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDV5876937.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            9631..11115
                     /locus_tag="RJE42_001517"
     CDS             9631..11115
                     /locus_tag="RJE42_001517"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDV5876938.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGS
                     LLSIWVARHRYSKTFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            11128..13833
                     /locus_tag="RJE42_001518"
     CDS             11128..13833
                     /locus_tag="RJE42_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDV5876939.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMADSYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQYLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDAIQVNVSENDNANQS
                     HSVSLNDTQAVDENNSELNQVCTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNTIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVVIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDEDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            13995..14357
                     /locus_tag="RJE42_001519"
     CDS             13995..14357
                     /locus_tag="RJE42_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDV5876940.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
BASE COUNT         5369 a         2061 c         2855 g         4394 t
ORIGIN      
        1 aagattcaaa agaaacacaa atcaaaaaga gctttgcgaa aacgttagat atgtacccta
       61 ttaagaatct cgaggattta tatgacaaag aaggatatcg tgatggtgag tttgaaaaag
      121 gcgataaggg gacgtgggtt atcagttcag caatggttaa acagccaaaa ggtaaaatta
      181 tgagatcaag aggtatgtat ctatttttaa atagaaatac aagaacggca aaaggatatt
      241 ttattgtaga tgtaacaagt aaggatacat taaaaaagac agaagataaa gaaaagcgtt
      301 atccagtaaa gatggtaaat aataaaataa taccgattgg tccaattaat gacgaaggca
      361 taaaaaaaga aattgaaaac tttaagtttt tctcacaata tggtgatttt aaaggattaa
      421 agaattatca aaatggtgat tattcatata actcagaagc accttcttat tctgctaaat
      481 ttcaattaag taatgatgat tataatgtaa aacaattacg taaaatgtat gacattcaaa
      541 ccaaaaaagc tcctaaattg ttgttgaaag gtactggaga tttaaaaggt tcatcaatcg
      601 gacataagga cattgagttt accttcgttg agaatcaaga agagaatgta ttttttactg
      661 atagtttgga gtttacccca agtgaggact attaaatatg atgaaaaaag gggatacgga
      721 caatgaataa ttttaggcaa tgcgcgttgt gtataggtac atcggtctta attctattag
      781 tgtcaggttg cagtggcgta tttgacgctc ctgaaggttc aaaagaaata caaattaaaa
      841 agagctttgc gaaaacatta gatatgtacc caatcaaaaa tcttgaagat ttatacgaca
      901 aagaaggata tcgtgatggc gagtttaaaa agggagacaa agggacgtgg gttgttagat
      961 ctgaaatgat aattcaacca aaaggtaaaa gtttaacttc aagaggcatg acattataca
     1021 tgaatagaaa tactaggacc acaaccggtt atttttcaat agaagagata gattcaagaa
     1081 aaagtcttga tgaaagagaa acagaaaaaa agtatcctgt gaaaatgaaa aacaataaga
     1141 ttattccaac tgaggagata aaagatgaaa agttgaaaaa ggaaattgaa aactttaagt
     1201 tttttgtgca atatggcagt tttaaaggaa tagagaatta tgaaaatggt gacatttcct
     1261 acaattctga agctcctatt tattcggcga aatataaact gaaaaatgat gattataatg
     1321 ttaaagaatt acgaaaaaga tataatattc caacagaaaa ggcgcctaaa ttgttgttga
     1381 aaggttcggg ggatttgaaa gggtcttcag ttggatataa ggaaattgaa tttatattcc
     1441 tagaaaataa aaaagaaaat atatattttt cagatggatt aaacttaatt ccaagtgact
     1501 aaaaataaca caaaggttga tagtgctcaa caaatattaa catgatagag agaagtgaaa
     1561 aaatgaggaa ttcaaaaaga gtagtattgt acataagcat tatggtgttg agcattttta
     1621 taattggttg cgatagatca agcgatatta cagaaaatca aagagaagat tcaaaggaag
     1681 aacaaatcaa aaagagcttt tcgaaaacgt tagatatgta cccaatcaaa aaccttgaag
     1741 atttatatga caaagaagga tatagagatg gtgagtttta aaaaggcgat aaagggccgt
     1801 ggacgatata ttcagacttt gccaaaagta ataaaccggg tgagctaagt aatgaaggca
     1861 tgttcttgag cttagataga aacacacgta cagctaaggg gtactacttt gtcaggacat
     1921 tttatcgcaa ggataagtta ccttatagaa aaaattataa agttgaaatg aaaaataata
     1981 aaatcatttt attagataaa gtagaagata aaaagcttaa acaaaaaata gaaaatttta
     2041 aatttttcag tcaatatgca aatcttaaag aattgaaaaa ctacagcaat ggtgatgtct
     2101 caattaatga gaatgttcca agttatgacg tgaaatataa aatgagtaat aaagatgaaa
     2161 atgttaagca attaagaagt cgttataata ttcctactga taaatcaccg gtattaaaaa
     2221 tgcatattga tggtaatttg aaaggaagtt ccgttggaga taggaagtta gaaattgatt
     2281 tttcgaaacg tgaaaatagc catttatcag taatagattc tttggattac cagccagcga
     2341 aaactaataa agatgatgaa taatgaggat ggtgtgtaac aatgaagtct ataaaaagga
     2401 ttggattgtg cataagtctg ttgattttaa gcatttttgt aacatcttgc gatggtgata
     2461 ataagatcac tggagattca aaagaaacac aaatcaaaaa gagctttgcg aaaacattag
     2521 aaatgtatcc aatcaaaaac ctcgaagatt tatacgacaa agaaggatat cgagacggag
     2581 aatttaaaaa aggtgataaa gggacttggg ttattagatc tgaaatgaaa atccaattaa
     2641 aaggagaaaa tctggaattt aggggagtag ttttagaaat taacagaaat actagaacgg
     2701 ctaaagggaa ttatattgtt agagaagttg ttgaagatag cgatggaatg acacacaatc
     2761 atacaaaaat gtatcctgta aaaatggaaa ataataaaaa tcattccatt aaaacaaatc
     2821 gttgatgaaa aagtaaaaaa agaaattgaa gaatttaaat tctttgtaca atacgggaat
     2881 ttcaaagaat tggaaaacta taaagacgga gaagtgacat ataacccaga agcaccaata
     2941 tactctgcac aatatcaatt gaaaaacagt gattacaatg tagaacaatt acgtaagcga
     3001 tataatatcc cgacgcaaaa agcgcctaaa ttattattga aagggtcagg taatctaaaa
     3061 ggctcatcag ttggatataa aaatattgaa tttacctttg ttgaaaataa gggagaaaat
     3121 atttacttca cagatagtgt ctactttaat ccaagcgagg ataaatataa ttactaatat
     3181 aaaaaataac gttagaacat ttaataataa agttaaatga attacatgta ttaaattgaa
     3241 taagtcagtt ttgtttgata acataaaagt gtaataatca ctattttatt aagttgtatg
     3301 aaatattcaa atgtgtataa aaataaagca gagagatgtg aaatgatgga gtatataaaa
     3361 aaaattgctt tgtacatgag tgtattactt ttaatcattt ttattggggg atgtggaaat
     3421 atgaaagatg aacagaaaaa agaggaacaa acgaataaaa cagattcaaa agaagaacaa
     3481 atcaaaaaga gttttgcgaa aacgttagat atgtatccaa ttaagaatct cgaggatcta
     3541 tatgacaaag aaggatatcg agatggtgag tttaaaaaag gtgataaagg tatgtggacg
     3601 atatatacag attttgccaa aagtaataaa gcagacgaat taagtaatga aggtatggtc
     3661 ttatacttag atagaaatac acggacagca aagggacatt attttgttaa gacattttat
     3721 gaaaaggata aattcccaga tagaaaaaaa tataaagttg aaatgaaaaa taataaaatt
     3781 atcttattag acaaggtaga agatccaaaa ctaaaaaaga gaatagaaaa ctttaaattt
     3841 ttcggacaat atgcaaacct taaagaattg aaaaattaca acaatggtga tgtctcaatt
     3901 aatgagaatg ttccaagtta tgacgcaaaa tttaaaatga gcaataaaga tgaaaatgtt
     3961 aagcaattaa gaagtcgtta taatattcct actgataaag caccggtatt aaaaatgcat
     4021 attgatggta atttgaaagg aagttctgtg ggttataaaa agttggaaat tgacttttca
     4081 aaaggtgaaa aaagcgattt gtcagtaata gattcattaa atttccagcc ggcgaaggtg
     4141 gatgaagatg aaagataaat acaaaataga tccaggacct attaaatata atactgagga
     4201 aactacagct atttctaaaa taagttatga aattgaaaat gcgaaattat atggaattaa
     4261 aagtagaaca ataggtagac aaatagatca attaaaagaa gcggaaaaat tcccttctaa
     4321 tcttgaatat gttgatagtt acactgattc tcttactgga gtaacaactt ctgctttttt
     4381 aaataaagat acaggcaaag taactctcgg gatgactggg actaatttac aagacgaagc
     4441 ctttaaaaag ttaaaagaag gtgaattttc aagacaaaat gttaccaatg ctttggaaac
     4501 agttaaagat ggatatgcag atcttaaaat attatattct cctgcatctg atcaaaacta
     4561 tagatatgcg aatacacaag aatttataaa taaaataaaa agtaagtatg acattgattt
     4621 tattactgga cattcactag gtggaagaga tgcggtagtt ctaggaatga gtaatggtat
     4681 tccgaacatt gtggtttata atccagctcc tatttctata actagtttga atcctaattc
     4741 cccagatgga aaacgattgt tagagttata taaaaattat aaaggtaata ttactaggtt
     4801 tgttgcagaa aatgatgcat tgacagaaaa tctgaagaaa tataagcatt atgttttttt
     4861 cggtaatgat aaagtcttta aaaatggtaa aggtcatgaa atggaaggct ttctgaccga
     4921 agaagaacaa aaagctataa aaaaagaact taaaaaacta caaggttatg cagaagaaaa
     4981 taataagtca tttgtaaaga attcaaataa tgctatctct aaattagcta gtatagaatt
     5041 acttagagca aatatgatga ctacaaatgg aggaggatta tcttcttcgc agcaaaaagt
     5101 tttagaaagt ttaacagctt taacaattgc gcagtcattc agtcaactga tagacgatga
     5161 aattaatcaa atcaaaaaaa tgtataatga aaagaaaaag aaatttggaa aaaattggga
     5221 agacgctcaa aaagctggaa aagctgtagg tgaagatttg agtgtaaatg gagttctcaa
     5281 tgctttagat gaaggtcaag tgaatgaaag tagtatggta agagaacctg aacaaatgat
     5341 atctgcaaaa gaaagacaac tttcaacgat agggtcttct gtatcaaatt atattatgag
     5401 agttaaactc agtattaatg aaatcgttga taaagatcaa gtgcttgcat cacaaatagg
     5461 tggattacta tgatgtttaa tcaaattaat aataaaaatg aattagaaga atcatatgaa
     5521 tctgagaaaa aacgtataga gaatgaactg caaaatttaa atgaacttag gcatagaact
     5581 cgaaaagaaa atgaacgtag ttatgatgtt tttcaatatt tgaagcatga aatgaattat
     5641 agtgaagatg cccaaaggaa aatgacgaga aatatagaag cgtatgagca agaaatcaat
     5701 gagataatta gaaagcaaga atggaaatta gaagaatata aagaagactt aaaaaagtct
     5761 tatgaaaagc agttagataa actaagtgac tgataatttg gaggagatta aatgactcta
     5821 atagaaccag atatgaactt aagaatgcca gatataagta ctacagtaga aacacttaat
     5881 ctcatatcta aaatggaagc gcaaaaagaa aatattcgca cagttattgc acctgaacat
     5941 aagcataaat ataaagatat tgaaaatggg ttaaaaggtg aagaaaaagt attaattgaa
     6001 caaatggcgc agcattgcga agcttttaaa gctaatttta aaggcgcagc tcaaggagat
     6061 tgggttaaaa gtgctatgtc tgagattgac agcattaagg atgacctgaa aaaaattaat
     6121 agttaaacta catatcatat taataaataa agctcccttc aaaattttca ttttttcaat
     6181 gtctactttg gagggagtac ttcagtataa tcacaactga caccaggccg cttcttttta
     6241 atttattcca ctttaaagta acgctatctt aatggaaata tcacactcac aattgtaaaa
     6301 acacaactta aattactgtc ccgcattaaa catatccaca ttccaaacag caacaacaaa
     6361 atcctcaagt accaaaaacg atgaccgctt tatcaaaatc aacgcaagtt aacctctccc
     6421 gaactccccg ctatagatca tcatcacctt acaaaccata aagcgctgca gttaagtatt
     6481 gaatggcata gccaattgaa atgaaaatag caatcgttat aatagcacta ccaatggtat
     6541 ataaagtatg tttcgtagat aatccttcag tctttgaacc aaaaacactt agtagaataa
     6601 atgcaatacc aatgatatat aaaactaaaa tgacaaatag catgtttata cacctcgatt
     6661 ctctttcgtg agatatatct ttgtctccat tataaagtaa agcgctcaat aatttaatta
     6721 agtatactat ttttttatta tcgaagattg cgaaagacga aatgctccta tataatgtaa
     6781 aacggaatta ttcctatttg caaatgaacg aaacaaataa attgaagtga gggtatatat
     6841 atggctaaaa ttccagttac ggtattaagt ggttatttag gctcggggaa gacaacgttg
     6901 ttaaatcata ttttacaaaa tcgagaaggt cgacgtatcg cggtaattgt aaatgatatg
     6961 agtgaagtaa atatcgataa agatcttgtc gcagatggtg ggggactatc gcgtacagat
     7021 gaaaaattag tcgaactttc taatggttgt atctgttgta cacttagaga cgatttatta
     7081 aaagaagttg agcgtttagt gaaaaaaggt ggcatcgatc aaattgttat tgagtcaaca
     7141 gggatttcag agccagtacc tgttgcacaa actttctcat atattgatga tgaacttggc
     7201 attgatctta cagcgatttg ccgtttagat acaatggtta cagttgtgga tgctaaccgc
     7261 ttcgtacatg acatcaactc agaagattta ttgatggatc gtgatcaaag cgttgatgaa
     7321 acagatgagc gttcgattgc tgatttatta attgaccaag ttgaattttg tgatgtattg
     7381 attattaata aaattgattt aattagtgaa gaagaactag cgaagttaga aaaaatgtta
     7441 agcgcattgc aaccgactgc taaaattatt aagacaacaa attctgaagt agatttaaaa
     7501 gaagtcttga atacgcagcg ttttgatttt gaaaaagcga gcgagtcagc aggatggatc
     7561 aaagaacttg agtctggtgg gcatgcatcg catacacctg aaacagaaga atatggtata
     7621 tcatcgtttg tatataaacg tcgtctacct ttccatgcta aaaggttcaa tgattggtta
     7681 gaaagcatgc caaataatgt cgttcgatca aaaggtatcg tatggctagc acaatacaat
     7741 cacgtagcat gtttattatc tcaagcaggg tcatcttgca atattcatcc agttacatat
     7801 tgggtggcta gtatgtctga agcgcaacaa acgcaaatat tagcagaacg ccaagatgtc
     7861 gcagctgaat gggatccaga atatggcgat cgtcatacac aatttgtcat tattggtacc
     7921 gacttagatg aaggtgcaat tacaaaagaa ctcgatgcat gcttagtcaa tgcgcaagaa
     7981 attgatgcag attggcaaca atttgaagat ccatatcaat ggcaaattag accggcacga
     8041 taagttgaat gaagtgtaat catttttaaa ttgtggctta attgtttgca atttatgaca
     8101 attaataaaa agttaaggtg ttcttaggat atttttgttt tcaatctatc taaagtgtga
     8161 tagttttgat aaagcagaaa tttgcatttt atccataggg ctaggacata tatgtgtctt
     8221 agtccttttt atatttgcgt tgattaaaaa tgacaaaacc atcgtaccaa ctgttaattg
     8281 aaagtacaat cattgtttaa cggtcataaa aatgagtgaa gtaattgcag ctatcaatgt
     8341 actattactg cttacagtca cacgagcgtt tcagactaaa cgtgcaacga aattctgtac
     8401 gcggccaata tatcttgcgc tagatcaaga agatcattaa gattttgcat gccaaatcat
     8461 gaatgtctca gacctcgagt atcaaacata aaacacgtca caaaaatata ccgcgtcata
     8521 ctacaaaaat cacgaatatt tcgtacgtcc caccaagaac atcctataca aaaaacacca
     8581 agaccattta tttgcaacaa actacccaga ctcacctcac atcaataaat caacacaaag
     8641 caaagccacc atccctattg gtatagtggc ctgagaattt taagtattca attcgcttaa
     8701 attattttgc gaaaatgtcg ataattgctt tgatgatttt aatgatagta cctacaatag
     8761 ccatcgtttt gtcctcctgt atgttgattt aacatagtga tgagttgttg attgttaatg
     8821 tttgagatta gttgttacct aaaaatttac caagtaaatc tttaaagaat ttgaataatt
     8881 ttgctacgaa ttccatgtga atggccccct tcaaataaga tgttcatatc tatgagattt
     8941 ttataactta cttaccagtg aatttctcaa ttaatccttt aatgaattta atgattcctg
     9001 caatgatacc catgtgaaag acctcctttg tttgttatga aatcttattt accagtgaat
     9061 tgttcgatta agcttttgat aactttaatg atgccagcga tgatacccat taagattacc
     9121 tcctttgctt atgagttaac ttcattgtac atagttatct tgtgcgtaat tgattttttt
     9181 cctaacttga attgttatga tcataactgc gaatttttta ttttaaaagg acgggaaata
     9241 ctgcctaact gtacaggtgc atttacaaaa tctttacaat cattggttta atagaggtta
     9301 agctcatgaa tttgacatga agcagaaatt taattatatt ttatgttata agtttaaaac
     9361 gaataacacg ttaggtctcg tctaggcaaa gcatatttat gaagatgaat tgaacagaca
     9421 gaagtaacat tttatataca tatgtgcatg ggagcacgct taaagagaac aatgcaacaa
     9481 ggtatatcaa cgtaccgtga tgcagtgtct agttaaactg aaacctgcaa tagtaaattt
     9541 tctgccatta tgtacagaat ctactattgt aggtttttat ttttatacat aagcaattat
     9601 gagagagatt agaaataagg aggttatgca gtgttaagtt ttcaattgct attttcactg
     9661 tttgttattg cgcttatcat tgcattgata agtggcttgt tgtttttagc accagttatg
     9721 ccaatgagat atattaaatt acatttatac atactagtca tgccagtatt atttgcagtc
     9781 attggctttt tcggtattca tggtcaacat gtcttaggtc catttaaaat agatcgttta
     9841 tcttggttat tagctggctt tgtaatggcg cttggcttta ttattcaaaa gttttcaatg
     9901 cgatatttac taggtgatca tcattataga cattactttc cattgttcac tgcgattacg
     9961 tcgtttgcat ctttagcatg gatgtctgaa gacttaagac tgatggcact ctgctggggt
    10021 atgacattat tatgtttaac attgctgatg aacgttaatc gtttttggaa agtgccacgt
    10081 gagtctgcga aattatcaag catgacattt ttatgtggtt ggcttgcatt cgttggagca
    10141 attgtaacta tttatattgc gactggcgag tggcgggtac cacaacatat agcgcatccg
    10201 acatggtcat tgttgacgaa tgtactactt gtattagctg tcatgatacc ggcagcacaa
    10261 tttccatttc atcgatggtt gattgaatct gtaacagcac caacgccagt atcggcaatt
    10321 atgcatgcag gaattgtgaa tgcaggtggt gttattctaa ctcgttttgc gccgatattt
    10381 gataatggat ttgcgttatc attattactt atcctttcta gtatttctgt attgttagga
    10441 tcgggtatta gcttggttca agttgactat aaacgccaat tagtgggctc tacgatgagt
    10501 caaatgggct ttatgttagt tcaatgcgca ttgggcgtat attcagcagc gattattcat
    10561 ttaatattgc acggtatttt taaagcaaca ttatttttac aatcaggttc tatcgtgaag
    10621 cgattcaata ttccaaaaca agcatctgct aaagacgctt atggctggat tgtcatggga
    10681 cgtgtattag ctattatcgt ggcatttctg ttttggatga gtagtgacag aagtgcatat
    10741 gaagtgttaa gtgcactcat tctagcatgg tcattacttg tatcttggaa tcaaatggta
    10801 gcctttagta aagggcgtat ggcacgtctg gttggcatga ttttgatttc aattgtgaca
    10861 tttatctaca tcatcacaca taattatttt tacgatgtat tacaaaatat aacaacacat
    10921 gcgacaacac cgcctacgat tagtgtcatc attagtgttg tcatattaat atttggtagt
    10981 ttattaagta tctgggtagc gcgtcatcga tactctaaga cttttgcggt attgtacgtg
    11041 tggttagtta atctaggtga agcacgctcg aaagcgatag aaagtcatcc gaattatttg
    11101 aagaagtatt tataggaggt gaaaggtatg acaacacagt taaatatcaa ttcagtcatt
    11161 gaaaatgcga aacgtgttat tacaccatta tcaccgattt cgatttttgc agcacgaaat
    11221 ccatgggaag gattagaagc ggatacattt gaagatgtcg caaaatggtt acgtgatgtt
    11281 cgagatgtgg atatttttcc aaataaagca ttaatagaaa gtgctgtggc acgcggtgaa
    11341 ttagacgaaa gtgtctttaa tcaacttgtt actgatatgt tacttgaaca tcactacaat
    11401 atcccgcaac actacatcaa tctttatatt gataacatta aaacattaaa agacgtacct
    11461 gcatcatata tgaatcattc aaatgttgat gttgttgctg atctactatt agaaaaatca
    11521 aaacgtgata tggctgattc atatcatcac tatgatgtac gtccgatgag tgatgcaata
    11581 atagatgaac aaggtgagcc acttagcgaa caagtgaatc gtcaaatgat taaatggacg
    11641 aaactttata tcgatcaatt tctatcgagt tggacaatgc cgaagcgaga gcaaagtttt
    11701 taccatgcat ggttgcattt agcgcaacat gaccatagtt ttactaaagc acagcgccaa
    11761 gtgattaaag gcttacccaa tgatcctgaa atgacgatag agtcagtatt aactcatttt
    11821 tcaatagatc aggaagacta ccaagcttat gttgaaggac atcttttggc gttaccgggt
    11881 tgggcaggta tgttgtatta ccgttcacaa cagcatcact ttgaacaata tttgttaacg
    11941 gattatttgg caattcggtt agttgtcgaa caattgctag ttggtgatga gtttaagtca
    12001 gtcactaaag attgtgaaag tagatcggaa aattggttta agcaaactgt tgcatcatgg
    12061 tgttactaca gtgatatgcc tagcgatgta ttactacaac atgacgtcaa tgaaattcaa
    12121 acgtttattc attttgcagc aactatgaat aaaaatgtat ttaaaaattt atggctaatt
    12181 gcctgggaaa tgacatacga atctcagtta aaacaaaaaa ttaaagcagg tcatgagagt
    12241 gtggcgggcg cattagatgc aattcaagta aatgtttcgg aaaatgacaa cgctaatcag
    12301 tcacattcag tatcgttaaa tgatacacaa gcagttgatg aaaataatag cgagctaaat
    12361 caggtgtgca catcaacgaa agcgcaaatt gcattttgta tagatgttcg ttcagaacca
    12421 tttcgtagac atatcgaagc agcagggccc tttgaaacga ttggtattgc aggcttcttt
    12481 ggattaccta ttcaaaaaga tgccgtagac gaacaattca aacatgattc attacctgtc
    12541 atggtaccgc cggcatatcg cattaaagag tttgcggacc gttacgatat gaatgtttat
    12601 cgacaacagc aacagacaat gtcatcgatg ttttacacat ttaagttgat gaaaaataat
    12661 gtcatgccta gtttgttatt gcctgaatta agtggaccat ttttaagctt gagtaccatt
    12721 gtcaatacga ttatgcctag aaaaagtcgc gcgtctttac agaaaataaa acaaaaatgg
    12781 ttgaagaagc ctgaaacaaa gttaacgatt gatcgtgagt ttgaccgaac atcagactta
    12841 cctgttggat ttactgagca agagcaaatt gatttcgcgt tacaagcgtt gaaattgatg
    12901 gatttaaccg aagcatttgc gccgttcgtt gtgttagcag gtcatgctag tcattctcac
    12961 aataatccac atcacgcatc acttgaatgt ggggcttgtg gtggtgcatc aagcggtttt
    13021 aatgctaagt tattagcgat gatatgtaat cgtccaaatg tcagacaagg attaaaacaa
    13081 gcaggtgtag taattccgga gaccacggtc tttgcggcag cagaacatca tacgtctact
    13141 gatacgttgg catgggtata tgtgccagat acattatctt ctattgcttt agatgcatat
    13201 gaatcattga atgacgccat gccgatgatt tctgaacacg cgaatcgcga acgtttggac
    13261 aaactgccaa cgattggtcg tgtgaatcat ccagtggaag aagcgcaacg tttcgcgagt
    13321 gattggagtg aagtacgtcc agaatggggc ttggctaaaa atgcatcatt tataattgga
    13381 agacgccaat taacaaaagg cattgattta gaagggcgga catttttaca caattacgat
    13441 tggcgtaaag atgaagacgg cacattgtta aataccatca tttctggtcc tgcacttgtg
    13501 gcacaatgga ttaatttaca atattatgcg tcgacagttg cgccgcattt ttacggcagt
    13561 gggaataaag cgacacaaac cgtcacgtca ggtgttggtg tcatgcaagg taatgcaagt
    13621 gatctgatgt atggcttatc atggcaatct gttatggctg ctgatcgaac gatgtatcat
    13681 tcgccaattc gtttgcttgt cgttgttcag gcacccgact atgttgtagc tagactactc
    13741 gcgaataatg agcatttcgc taggaaggtg tctaatcatt ggctgcgttt aatgagtgtt
    13801 aatgaggaag ggcgttttaa aagttggatt taacgatgaa atgttgcgat gctaagtaac
    13861 taatatatca tcatatctac aatgttctag cttcttaaaa tatactaaaa atgataaact
    13921 taatgtaata gttgatgtcg tgataacgat acaaagataa agtaatcaat tttaatatta
    13981 ttggagttag tgatatgaaa agaacgaaag gtgaaatcga agctgaaatc agtaaagcca
    14041 ttacgcaatg ggaaaaagat ttccttggca gaggttcctt gtcggttaaa tcagacattt
    14101 taagagatat ggtgattatt agtttacaag gtatcttaac gccagcagaa tatcgtgtgt
    14161 gtagtacgaa tgaaggatta ttaaatatta aacgaacacg ttctgaatta gttgaatccg
    14221 gtgagcaaga tttgaatgat atcattttta aaattacagg tatcaaagtg atgagcttcc
    14281 atagtgattt aagtacagtt acaggtgaac gtattatcgt attcaaactt gaggataatt
    14341 tggaaaagca tatttaagaa ggttaggtat gcttctgctg gcttattaaa gctatagaag
    14401 tatgcttggt taaacagcgc aacgccatat atttgggtgt gctgtttttc tattatatga
    14461 tgtgtttatc acctggaagg atgtgtgact ctgaatatag cccgtgaaag gcatatgcca
    14521 taaaatttgc gaatggtgga cgaggcaacg caagcatatt tgttgcgaga catgtcaaca
    14581 atccttttac ataaagagtg tatgtatgac gtatgcatat aatcgataaa atatacctaa
    14641 aaaattaggg ataatgacca ttgttgcaat aagtttttc
//
LOCUS       DAPIDM010000059        14296 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000059 DAPIDM010000000
VERSION     DAPIDM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.059"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..250)
                     /locus_tag="RJE42_001520"
     CDS             complement(<1..250)
                     /locus_tag="RJE42_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDV5876941.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEV"
     gene            complement(308..1222)
                     /locus_tag="RJE42_001521"
     CDS             complement(308..1222)
                     /locus_tag="RJE42_001521"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV5876942.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVAAKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            complement(1224..2285)
                     /locus_tag="RJE42_001522"
     CDS             complement(1224..2285)
                     /locus_tag="RJE42_001522"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV5876943.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEKGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWEKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(2291..3571)
                     /locus_tag="RJE42_001523"
     CDS             complement(2291..3571)
                     /locus_tag="RJE42_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDV5876944.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            3762..5144
                     /locus_tag="RJE42_001524"
     CDS             3762..5144
                     /locus_tag="RJE42_001524"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDV5876945.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCNKN"
     gene            complement(5198..5773)
                     /locus_tag="RJE42_001525"
     CDS             complement(5198..5773)
                     /locus_tag="RJE42_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876946.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            6104..6958
                     /locus_tag="RJE42_001526"
     CDS             6104..6958
                     /locus_tag="RJE42_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876947.1"
                     /translation="MSFYVVLIIIIVALIGILILNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DRITAIFK"
     gene            complement(7103..7636)
                     /locus_tag="RJE42_001527"
     CDS             complement(7103..7636)
                     /locus_tag="RJE42_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV5876948.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            7775..7963
                     /locus_tag="RJE42_001528"
     CDS             7775..7963
                     /locus_tag="RJE42_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDV5876949.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKH"
     gene            complement(8085..8165)
                     /locus_tag="RJE42_001529"
                     /pseudo
     CDS             complement(8085..8165)
                     /locus_tag="RJE42_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
     gene            complement(8346..9827)
                     /gene="cls"
                     /locus_tag="RJE42_001530"
     CDS             complement(8346..9827)
                     /gene="cls"
                     /locus_tag="RJE42_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV5876950.1"
                     /translation="MQFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKIDHFIDGNNLYDQVLQDIKNAKEYIHLEY
                     YTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDEKYFPKKNKSFGNAPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRAAYEHDITKSKQLTEESYANRPLTVKFKESLAKLVSPIL"
     gene            complement(9885..10082)
                     /locus_tag="RJE42_001531"
     CDS             complement(9885..10082)
                     /locus_tag="RJE42_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876951.1"
                     /translation="MEQIKLKTFTAETLELLESNINEFLGSEEAANLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            10351..11376
                     /locus_tag="RJE42_001532"
     CDS             10351..11376
                     /locus_tag="RJE42_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDV5876952.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            complement(12054..12887)
                     /locus_tag="RJE42_001533"
                     /pseudo
     CDS             complement(12054..12887)
                     /locus_tag="RJE42_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(12963..13214)
                     /locus_tag="RJE42_001534"
     CDS             complement(12963..13214)
                     /locus_tag="RJE42_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876953.1"
                     /translation="MIIMRKLKEDDQVIVYEYIPQDKIEKGKGEITVNKLDSKVIDYK
                     LSKVENEKGILVYRDKSFHAILNFIDENKFPNEYIYAWY"
     gene            13626..13961
                     /locus_tag="RJE42_001535"
                     /pseudo
     CDS             13626..13961
                     /locus_tag="RJE42_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            13974..14296
                     /locus_tag="RJE42_001536"
                     /pseudo
     CDS             13974..14296
                     /locus_tag="RJE42_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         4782 a         2299 c         1990 g         5225 t
ORIGIN      
        1 ctacttcttc attagtcatg ttaatcgttt tactgccgtt ataactaata atatagctat
       61 gatgttcagg taattttaaa tctctagcag ttggaatcat accttcagta ggtctaccag
      121 atgctaaaac aacataataa ccttcatctt gaattgctgt taaataattt gcagtttctt
      181 ctgatatcac attatctgaa tttagcaatg tgtcatccat gtctaaaacg acaactttat
      241 aatttgtcat atcgcattaa atctcctctc tatttcacat tcaatactat tttacaatat
      301 aaagtgtcta ttggtataca attcgttctt taacaccact aacattaata tccactaatt
      361 cagaatgaca tgtaactaca tttctattta aagcgcgaac taattcgcca ctattttctt
      421 tgcgactaaa tattaaaata gttggtccag caccactaat tacagttgca taggcattat
      481 gttgactagc aattgttttc acttcatcaa attcagcaat taaatgctga cgatacggtt
      541 catgaaagcc atcttgttgc atgagtttac ctgctaattc ataattgtgc tgtgctaatg
      601 cacaaatcat tgtattacta attgcactac ttttaaccgc ttcactatgt gttaattttt
      661 gtggtaaagc acgtcttgat gcttctgttt ttagttcata agttggtatc gttacaatca
      721 catccacgtc tggtatgtcg atatgtgcaa ctgacgtttc tttcgagaca tcattataat
      781 acccagcgat taaaccacca taaatggtcg gcgcaacatt atcaggatgt ccttcgattt
      841 cagtcgctaa ttgtaatacc tcgtgtttag atagttggat atcaccaaaa taatttgcga
      901 tatatatagc tcctactaaa gcagaagctg acgaacctaa ccctcttgcc aatggaatat
      961 cacttctcat ttcgatacat aaattaggca agtcaacact atatttagca gcaacttgtt
     1021 gtgccacatg ataaataaag tttgtttcgt cagtaggtaa ttgcttcgat gcatcatcat
     1081 ggaaaatata ttcccatttt gtccctgatg tttcttttac agacagatgc aaaaatttat
     1141 ctaaagccat acctatagaa tcaaagccaa ctccaaggtt ggctgttgat gcaggaattg
     1201 ttaactccaa aacattcgac atgttataaa gctcctttaa tataatcgat aatgctatct
     1261 ttatcatttg gcaatggctt tatcggatta tctagtagtg aaatagcagt atcaggatct
     1321 tttaatccat taccagttaa aatcgcaact acttttttac cttgaggtaa tttaccttgt
     1381 ctatgcaatt taattaaacc tgcaatagaa gcattacttg ctggttcact aaagacacct
     1441 tctttagttg tcattaactg atatgcttct agaatttcat catcagtaac actatctatt
     1501 aatccatttg attctttaag agcattagtc gccttttccc aactagcagg attaccaatt
     1561 cgaatagcag ttgcaatcgt ttcaggattt ttaatgactt tattttgaac aattggtgat
     1621 gcgccttcag cttggaagcc aaacattttc ggcaattgtg atcctttagc ttcatgatat
     1681 tctttaaagc ctttccaata tgcagtaata ttacctgcat tacctacagg aatcgctaaa
     1741 atatcaggtg cttcaccgtc taattgttgc acaatttcaa atgagcctgt cttttgccct
     1801 tcgattctaa atggattgac agagtttaca agctcgattt cgccactttt tgcaatttct
     1861 tttacaattt ctaaagcttc atcaaagttt ccttcaatag aaacgatttc tgcaccatac
     1921 attactgctt gcgacagttt acctaacgca attttacctt ctggtattac gacgatagct
     1981 tttaaacctg ctctagctgc atatgctgct gcagacgctg atgtatttcc agtcgaagcg
     2041 catattacaa ttttcttacc tttctctttt gcttttgtca cagccattac cataccgcga
     2101 tctttaaatg aacctgtcgg attggcacct tcatacttca catataaatc tatacctagt
     2161 attttagaca tattttcaca atgaatgagt ggtgtatttc cctcgttcaa tgttaatttt
     2221 ggtgtatttt catttactgg taaatgtgct ttaaactctt ctactaatcc ttgccatctt
     2281 ctcattataa ttaaactcct tctactgggt atactttgtc aacttcgtat ccatgcttag
     2341 tgattaattc ttccggtgat gattccaatc caactataac agcggcatat gattgattat
     2401 cgtaatttgc aactcttaat gatcggtgga atggtaatat tgcctttaac tcattttcaa
     2461 aattttcaat tgaacctttg acatgattca ctactacaaa gaaattggat ttttctttaa
     2521 tattaatttc tgcatcagaa tccatcattt cccttgtttt atctgtcttt aattcaaaat
     2581 gtggtggcaa tgtgtgtaaa tctgattcaa agaataatgc tacattcaat aaatcactga
     2641 caacggcact acctgttgct aaactgcctg ctccttttcc ataaaacatc gtgtcaccaa
     2701 cggcatcacc aatgacataa atcgcgttat attcatcctc tacagctgct aattgatgct
     2761 ttttatcaat taacgttggt tctactgatg cattaacttt gccattttca tatattccct
     2821 taccaatcaa tttaatttta taccccagtt gatcggctac attaatatca gttaaagtta
     2881 caccactaat tcctcttcgt ttaacatcgt ttaatttaat gacttggtta aatgataaat
     2941 atgatgtaat gacaacttta cgcgctgcat ctaccccttc tacatcatca gttggatccg
     3001 cttcagcaaa accaagtctt ttcgcttcat caagtgcttc ctcaaatgtc gtttgctctt
     3061 tagtcatttt agataaaata aaattagagg taccatttaa aattcccata aattttgaaa
     3121 tattattcgc attcaaacca ttatttatgg catttacgat cggaatacca cctgctacac
     3181 tcgcttcaaa ctttaaagct acaccatttt cttctgctaa atcttctaaa agtttaagat
     3241 gtactgctaa taaatcttta tttgcggtaa taacatgttt tttattttta agtgctgttc
     3301 ttaaccaatc tacagttggt tcaattcctc ccatgacttc aacgataata tctaatgaat
     3361 catcatttaa aatttcatta acatcttcag ttaaatgata ttgactaata tttagcggtc
     3421 tctttttaga tttatctcga acaagaatat gctttatgac aatatcttta tttaatgtat
     3481 cttgaatttg ctgtcggttc tcttcgatga ttttaacaac accagatccg acagtaccta
     3541 atcctaataa tgctatattt aattttttca ttttatgtac ctccgtttgt gcttttgaga
     3601 aaaacaaatg tattattgaa aaacactaga tgatagtata gtatagaatt attctttatt
     3661 tttgtaaagt tcgtaatatt caaaatataa atacataata tcattcttac ttacaaaact
     3721 gaatacaatt ttctgatttt ttagaaaggt tggcatgcac tatgaaagtt tccaaattcg
     3781 gtggtagctc cgtctccaat gctactcaaa ttaaaaaggt tttaaatatt gttaattctg
     3841 atccagagcg aaagattgtt atcgtttctg ctccaggtaa aagacatgat aatgatatta
     3901 aaacaactga tttgttaatc agattatatg aaaaggtcat taatcatctt gattatcatg
     3961 ataaaaaaag agaaattatt cagcgttatg atgatattgt aaaagaattg caaatggatg
     4021 aaagtatttt acggacgata gatgtgactt tggaacatta tattaatcaa ttaaaaaatg
     4081 aaccgaagag actattagat gcattacttt cttgtggtga agattttaat gcgcaattga
     4141 tagctttata taataatagt caaggtatac caacaaaata tatatcaccg aaggaagcgg
     4201 gtatttttgt tactgacgtc ccacaagaag cacaaatatt agatagtgct tactctgaaa
     4261 ttaaaaaatt aagagattat gatgaaaaac taattatccc aggatttttt ggcgtatctc
     4321 atgaaggata tatagttaca tttccacgtg gcggatcaga tataactggc gccatcatat
     4381 caagtggtgt tagagccaca atttatgaga atttcaccga tgtgtctggt atttataaag
     4441 ctaatccaaa tatcatcaaa gatcctgagt taatcgaaga aatcacttat cgagaaatgc
     4501 gtgaattatc ttatgctggt tttagtgttt tccatgatga agccttacaa ccactttata
     4561 aagatcgcat ccctgtggtg attaaaaata cgaaccgccc tcaagataaa ggtacattta
     4621 tagtgcatga tagagaaata aatgcgaaaa atgtgattag cggtataagt tgtgataaag
     4681 attttactgt tattaatatc aaaaagtatt taatgaatag acaagtcggc tttactagaa
     4741 agatattagg tgtcttagag gataataata tttcatttga tcatatgcct tcaggtattg
     4801 atactatcag tatcgttatg cgttcaaaac aaattcaaaa taaagaaaca aaagtattaa
     4861 atgaaattcg tcaaaaatgt gatgttgatg agctaagtat tgagcatgat ttagcaattt
     4921 taatgattgt cggcgaaggt atgaataaag tcattggtac tgcaaataaa attacacatg
     4981 ccttagctga atcaaaaatt aacttaaaaa tgattaacca aggtgcttcg gaaatttcga
     5041 tgatgtttgg aattcatgta gatgatgctg aaaaagctgt attatctaca tatgaatttt
     5101 gctatcacgg ggttgcatta aaaaacttgt gtaataaaaa ctaatataaa agcaattcgt
     5161 aacacatgta ttcaactgcg ttatgaattg ctttttctta attatttatt gttctctgga
     5221 ttgcatcctt aatacgttgc tcacctacta caaattttaa aataaaaaat tgatagtagc
     5281 ttaatgcatc tatgacacgt ttataattgg aataatgatg atactgatgg tgttgcatta
     5341 gatcatacat tagaacgatc tccggtttaa tataatccac atcccaattt aaactatgga
     5401 agtatatgtt actcttaggt atacgtatat tcggatccaa tctaaaaatc cattcatcgt
     5461 ttactacatc ataaacgaaa atactcatta ttgttgtatc atcatattgc acataaactt
     5521 ttgcaatttt tgaaagtgat aaatcttctt tagctattgc ttgatcttgc ttatctatgt
     5581 atagtaacgc atatgattgc ggaataactt tcacaatatc acttaaacgc ttacgttcca
     5641 cgcatatttc aacaatagaa ggtaatttta attcatcatt taagaaaagc tgcatggcaa
     5701 cttcaccgtg aaatttaaat aattgcgatg atgttttcat taaattgtcc actaattgtt
     5761 ctttagcatt catttcattc actctcaaat acacctccta agcaagcgct ttcacaaata
     5821 tcattataca ttttaaaatt aatgaattca atcttcaaaa tactatgagt gacctaattc
     5881 tgtcatttaa atgaccgtta taacataaat tcgacataaa cttgaattca cgaatctttc
     5941 acgtcatatt cattgaacat caatatctgt cagtttataa tttgattata acaatgatga
     6001 actaaaatca actaataaaa atgtgggaaa ttgaataaca attaatcttt attaattatt
     6061 attgtataca aagttaaaga agtcaaaaag gattggtgaa aaaatgtctt tttatgttgt
     6121 acttatcatt atcattgtag cattaattgg tatattaatt ttaaatcaaa ggtatagtaa
     6181 tagtaaaata gataccgaag tttatgcaag aaagcaactt attaaaaaga acaaagcatt
     6241 aagtgcagaa aatgctgaat taagaagtca aatgctgagt tctaataatg atgttggcca
     6301 ccatgcatac aaaaatgcta aaagagaatt acgcaagatt ttagacagct atctcgaaaa
     6361 tggtaaatta aaatattatg atattatcgt tacaagcaat ttagctacta aacatccgtt
     6421 tttcgagtat gcacgttcat ttgattttat cattgtttca gatatcggtt taataaacgt
     6481 tgatgtaaaa agttggggtg aaaaaacatt ttatcacttt gatgtacctg atgagcatga
     6541 tacagaaatg agcaatagta atattgaaaa agttgtcggt cattacatta gtcagcaata
     6601 tcatgatcag tttaattcat caagaaagtc tatctatact tttacagaaa cagttcaacc
     6661 aaatcgtgtt atttatgatt tttatgatta tgatccgtat caattagcag caaataatgc
     6721 taaagcatta aaggatcata ttgaacaaaa ctttaacttt aaagtccaaa gtaccggtgt
     6781 tatttacttt agtgatggta cagttaatat cattcaaggt tctgaagaaa gagataaata
     6841 tgttgataca gtttcaacaa aatcttcgtt acgacgtatt attagcgaag ccatagagct
     6901 atctaaacac cctttaaata aagaacaggt tgatcgaatc acagctatat tcaagtaaaa
     6961 tcaaaggcat aataatccat tacgctcgat acgcactatg gttattatgc ctttaaatta
     7021 tttctatata taaatttatg gtaataataa atcgatgctt caagtatatt ttactttaat
     7081 atatatataa tcacctattt aattatttac tccaaatatt taatttctgt tgtttagctt
     7141 tattttgtgc ttctataaat acatttctat atttgccatt tggtgaaaaa tacttctctc
     7201 tagcaagtcc cttttccact aattccttat tgtacatacg atctttactt atccatacat
     7261 acgccaatgt tctaccatag cggtcttgct tttctttatc atattctaaa taaacatctt
     7321 gatttgttaa tgtcttctta ctataatttg atgcttcttt gccaaatggt tgtacaggcg
     7381 tattcggttt caccgtttct ggcgtatcaa cccctataag cctaacttta atttctttac
     7441 cattttgatt tgcaataaat gtatcaccat ccacaactct ttgaacatgt actttatctt
     7501 taccatttaa atcttgtaca gtttcatgat ttgttccctt tttaaaagga cccgtatgat
     7561 tcataaattg aaatgctaat acacctacaa taatgatggc taccacaatc atagcaagcg
     7621 atttatttga cttcattttg ccacctactt ctcttttttc attccgatta atcatataaa
     7681 agttaagaaa tatcgtcaat agccatatct acatatttca atattttgga aagctatagc
     7741 atgatataat gttattgtta aggagtgaaa atgaatgaac atttcaaatg aaaatacagt
     7801 cacattaatt gcagtaatta tcgcaattat cattggtatt ttcctacaaa tatttttcaa
     7861 attaccatta atagtgacgg cagtactatc tattttattg ggtatctttg taggctttat
     7921 cgtttacctg attgtttcgt tttataataa acgaaaacat tagttaacaa atctaacttg
     7981 cgagtaccaa aataaaagta ttgagtaagt ttttgattat tgactcactt tcaaagtctc
     8041 ttaaatcaaa acactcaata cttttttgtt tgtatttaga tccagccttt ttcaatgcca
     8101 actgaaatgt tttttaaaac acgctttttc ttaaatgatt tattaatatt agatatttga
     8161 atcaaatgaa ccacctctta atattttatt aatttaagat tagtaaattt attcgattta
     8221 aaataggata ctttgtcatt ggttcaaaat gacatttgtc acttcaatgg tttacataat
     8281 ataattatag tatttaatta caaaaataaa aaaagcctca ttaaaatgag acttgaaagt
     8341 attctttata aaattggcga aactaattta gctaacgatt ctttaaactt aacagtcaat
     8401 ggtctattag cataagattc ttcagtaagt tgttttgatt ttgtaatatc atgttcataa
     8461 gccgctctta aatctttagc aagattttca tcatatacaa aggcatttac ttcaaaattt
     8521 aattcaaaac ttctaaagtc catatttgct gtgcccactg atacgatttc atcatcaatt
     8581 aaacacattt tagaatgaat aaatccattt tcatacgtat aaattttaac accgctggat
     8641 aataagtcag atgcatttga aaatgtcgcc caataaacta aaggatgatc tggcttacat
     8701 ggaatcatta gatgaacatc tacccctgat ttagctgcaa ttttaatggc atttatatat
     8761 gagttatccg gaatgaaata aggcgattgc aaatataccg acttcttagc gctcataatc
     8821 atttttgtat atccatattc aatttgatgc cagtcactgg cgggaccact tgcagcaatc
     8881 tggattggcg cattaccgaa tgatttattc tttttaggaa aatatttttc atcatattca
     8941 aattgaggtc gatgtgcttg cgaattccag tctaaaataa atcgcaactg cagtgcatca
     9001 accgcatccc cttgtatacg taaatgcgta tctctccaat accctaattt tcctaaacct
     9061 agatattcat caccaatgtt aaatcctccg acataaccta gttgaccatc gattacgatg
     9121 atttttctat gatttctatt attcattctg aaattcaata acggtaattt agaagcaaaa
     9181 aatgcttcaa cttctccacc taaggattta aaatgatcaa aattagccat cttaactttc
     9241 tttgaaccaa cgtcatcata taaaattttc acttctagac cttgtttcaa tttctcttct
     9301 aacgctttta aaattctttg gcctaagcca tctagagcga atgtataata ctctaaatgg
     9361 atgtattctt tagcattttt aatatcttgt aaaacttgat catataaatt atttccatca
     9421 ataaaatgat caattttatt gttttcagtt aaaaaaccat cttgatccat caaaagcata
     9481 cgtactaaat catgatgttt ttgaacttgt ttgttatcag taccataatt acctttatca
     9541 aagctttcta tttgttgttt taaaagtcca tcgaaatccg ttaacacgtt accattgttt
     9601 ttattcaatt tgcgtgccga aacggttcta ccaaaaaaca agtaaagaat aaaaccaatc
     9661 aatggtaaga caaaaagtac aaatagccat gcccaagttg aactcgctgt acgcctattt
     9721 ctttctaaaa agataataat aaaagccaat actaaattaa tgatgaatcc aatggctaaa
     9781 ataatagtaa ataacgttcc taaatcgttt gaaaatgtaa attgcatagt cttttctcct
     9841 ataaagaaag gcacaaaaat attttttgca cctttcgttc cagattattt atttacagat
     9901 aaagttaata ttgcattaaa ctcttcttca ttatttggaa atgttctttc ttcaatttct
     9961 tttattgtaa tatttactaa tttcaaattt gcagcttctt cagaacctaa aaattcgttg
    10021 atatttgatt ctaataattc taaagtctca gctgtaaaag ttttaagttt aatttgttcc
    10081 atttaaatcc cctcttttct ctatttccta aatcatagaa cttttaacag taaaattcaa
    10141 tcattaatat atgtgtttcg gcacgcaaag taattgtggt aaaaaattaa ttaattttca
    10201 taatgtattg tttgatgtat aatatattta ttcaacttcg cgataatgcg atacaaactt
    10261 taaccattca taatacattt taaatatcga actagttttg aaattggttt atcttattaa
    10321 aatgttgtat tagttttagg gggaaatgta gtgatttcat ttgaaaatga ttatttagaa
    10381 ggtgctcatg aaaaagtatt aaaacgatta gtagatacaa atcttgtaca agcttcggga
    10441 tatggttttg atcaattcac agctcaggca attgaaaaga ttaaagatac aattgattgt
    10501 ccaaatgcta ctattcgttt tttagtaggt ggaacacaaa ccaatcaggt tgttattaat
    10561 tcaatgttag aaagttacga aggggtcatt tcagcagaca ctggtcatgt tgctgtgcat
    10621 gagggcggtg caattgaata tagtggacat aaagtaatta ctataccctc taaggaagga
    10681 aaagttagtg cttcagatgt tgaaacttac atggagacat ttaaaagtga ttttaaaaag
    10741 gaccatatgg tattcccagg aatggtatat atttctcatc ctacagaata tggcacatta
    10801 tactctaaat cagaattgga agagttatgc aaagtatgta agcaatatca gcttccatta
    10861 tttatggatg gtgcacgatt agggtatgga ttgatgagtg atcaatcaga tatgaccatt
    10921 aaagacatcg ctaaatattg tgatgtattt tatataggtg gtacgaagat aggtgcatta
    10981 tgcggagaag caattgtttt tacgaagaat aatgaaccga aacaatttac cactcgaatc
    11041 aaacatcacg gtgccctttt agcaaaagga cggttaacag gcatacaatt tttagaatta
    11101 tttacggata atttatattt taatataagc agacatgcta tcgaaatggc aaataagatg
    11161 aaagacgggt ttataaataa aggctatcgt ctttactttg attctccaac caatcaacaa
    11221 ttttttattt taagtaacga gaaaatagca gagttagaac aaaaagttaa atttgctgtt
    11281 tgggaaaaat acgatgatca acatcgtgtt gttagattcg caaccagttg ggcgacaacc
    11341 gaagaaaatt taaataaact attagaatta atctaataat gctgttatat gtcatctggc
    11401 aaatcattta ttaattaatc aagatcagtt gagtagttgt gtttatttat atatgaaact
    11461 gctcatttgt agccgttgat gatataaaat tacattattc caaaacaagg tactacagat
    11521 tattgagtat cttgttttat tttatttata tatttaaaaa tgttaatttg tgctgtattt
    11581 actttaattt gacgagattt gagaagtatt atgatagttt tagttatttt atgaaagtga
    11641 tgtattaaca atcgaattac taagtctata ttggtaggaa tatcgatgtt tagtttaaat
    11701 gaaatgcact atttaagatt taatatggag atgtttgtga cttttcgatg attaagattt
    11761 ttataggtgt gcatcttttc aaataaactt tgtgtttaaa attgagctta ggaaatcaat
    11821 aggtttagat gaggatattg ttgaaggtat gtgtcttgta tccttagttg ttaaaaaagt
    11881 gtataaaaat agcaccgctt tctctttatc tgtgtagaaa ggatgctatt tttctaattt
    11941 ttgttcttta ttcttatttt ctaattctat tgctttcttg tattctttat ccaataattc
    12001 ttgtgactgt ttattagtta tttcaccatc taactgtgca aaatacattt taatcaccta
    12061 actttatata ttatgttacc actattatat aatctcttta gaatcatatc aatatcctta
    12121 tgattgaatt tttttatatt attattttta ttatattttt taatacaatc aatacaagtt
    12181 gatttgcaat attcttttgt cttagttaag tactttgttt gacctttatt tgtaacaatt
    12241 gtcagtgttt taatagaatt attaaatata aataaataca agtctgtaag agaaaaacta
    12301 gactgcccag gatggttatg taacataaca attgaattcg gtttactgtt aaataacaat
    12361 tctgtttctt tttcccctgg tgcaaaagat acactatctt gatcaccata tacttttgta
    12421 actttaccat cttttaataa ataagctact tcattgctat cattgttttc tttggcgtct
    12481 ttaagcaagg ctttatgttg ttcttgtata aattgatttt cttctttggt atgtgttgga
    12541 atatcaacgt atcttacttt atcaatcgct tgatcagtaa tatatatttt ctttcctaat
    12601 actttagctt gttgtaattc atctttggtt gtgtcatctt ctacttgata tttaccttca
    12661 cgctctttaa agaacttgtc tcgccaactg ccgacatgtg gcactgtggt acttctacac
    12721 caaggatgca taggtggcgc attcacacct ggtatcatat ctttaacttt aaatattttt
    12781 ccgttgagtg aatgacataa tttagatgtt ttactatcta ttttggcaac atatttatat
    12841 tcgccatctt caccaagttc ttttaaatat gttaactttt gtgattctgc tatttcagta
    12901 aatagttaaa aaagcatata aaaatagcac cactttctct ttatctatgt agaaaggatg
    12961 ctttaatacc atgcatatat atattcattt ggaaatttat tttcatcaat aaaatttaat
    13021 attgcatgaa atgatttatc tctataaact aaaatacctt tttcattttc aacttttgat
    13081 aatttatagt caattacctt tgaatctaat ttatttactg ttatttcacc tttccctttt
    13141 tctattttat cttgtggaat atactcatat acaataactt gatcatcttc ttttaacttt
    13201 ctcataataa tcaatcatat caccctattt ctcaatataa ctagaaaaat tgtattttct
    13261 ttctacaatc ccatgtgctt ctctatagta ataattatat ttattcatca aataatgttc
    13321 aagtgcctct tgacgaagta gtattaaatc ttgtttttta ttgtttttcc ttctcttaaa
    13381 cattgccaac ttactgcaat attataatct ggatagaaat tattaataca gttatctaat
    13441 catatttatt cttaaacaaa tgttcatacg ctcttttaac taggttttaa ttcaaattgg
    13501 tatttttaga aattatatct acttctatat ccttattcct ttgacgtact gatttataat
    13561 acattttttc ttttttaaat tatataatca tgttttaact caatcttcga ttggagctta
    13621 tttaattgag acatgacatt tatgaaggag tgctatttta cattacgaaa ggtattaaac
    13681 caaattatgc tgagcttggt cgacaatata attgtgatcc aataacagtt aaaaaatatt
    13741 atgaagcagg aaaagaaaat gaattagaac gattgaagaa aagacaacaa aataagtaaa
    13801 catcaaaact agattcattt aaagaaatta ataataaaaa aattgaatta ggatatacag
    13861 ctatggccat ttttaaatat atagaggaaa agggatatga aggtaaatat actattctac
    13921 gagaatactg taaaaataaa atacaaaatg agactaaaaa aataacattt tttgaagata
    13981 atataaatat ttgtttctta gataaccgtg ttgttggtaa aacacaccta acaatctcat
    14041 taggaattga ggcatgtaaa caaaatataa agactagatt ctatactttc aaagaattaa
    14101 tagacttatt aactgtcaca gattccaaag aaatcattaa taaaacatta aaacaattaa
    14161 gcagaataga gttacttatc attgatgaaa tttgttattc tgccatcacc aaagaacaag
    14221 ctgatttatt ctaccagtta atgtcactaa aatatgaaat gaaatcaaca ataattacga
    14281 ctaatattac attttc
//
LOCUS       DAPIDM010000060        13990 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000060 DAPIDM010000000
VERSION     DAPIDM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.060"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(242..748)
                     /locus_tag="RJE42_001537"
     CDS             complement(242..748)
                     /locus_tag="RJE42_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5876954.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAERDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            861..1784
                     /gene="graX"
                     /locus_tag="RJE42_001538"
                     /gene_synonym="apsX"
     CDS             861..1784
                     /gene="graX"
                     /locus_tag="RJE42_001538"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDV5876955.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTTVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIVETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            1800..2474
                     /gene="graR"
                     /locus_tag="RJE42_001539"
                     /gene_synonym="apsR"
     CDS             1800..2474
                     /gene="graR"
                     /locus_tag="RJE42_001539"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDV5876956.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGEQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEIGMDSAIETKVGK
                     GYMAHE"
     gene            2467..3507
                     /gene="graS"
                     /locus_tag="RJE42_001540"
                     /gene_synonym="apsS"
     CDS             2467..3507
                     /gene="graS"
                     /locus_tag="RJE42_001540"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDV5876957.1"
                     /translation="MNNLKWVVYFLKSRKNWIFWILFLNILMLGISLIDYDFPIDSLF
                     YIVSLNLSLTLIFLILTFFKEVKLYRHFEKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ILAHKDKVVDQQLQLKMHEQTITEFVHDIKTPVTAMKLLIDQEENQERKQALLFEWSR
                     INSMLDTQLYITRLESQRKDMFFDYVSLKRMVIDEIQLTRHISQVKGIGFDIDFKVDN
                     HVYTDIKWCRMIIRQILSNALKYSENYNVDISTELIDQHVALIIKDRGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVDSVKDQLGIQLQVTSTIGKGTTVKLIFPLQNEI
                     VERMSEVTNLSF"
     gene            3654..4415
                     /gene="vraF"
                     /locus_tag="RJE42_001541"
     CDS             3654..4415
                     /gene="vraF"
                     /locus_tag="RJE42_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDV5876958.1"
                     /translation="MTILEVKQLTKIYGNKKMAQEVLRDINMSVEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGHKLEKLSNKALSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKDTMLDRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLMKMNEEIKTTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGINYEF"
     gene            4405..6294
                     /locus_tag="RJE42_001542"
     CDS             4405..6294
                     /locus_tag="RJE42_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5876959.1"
                     /translation="MSFNEIIFKNFRQNLSHYAIYLFSLITSVVLFFSFVALKYTHSI
                     NITETYPIIKEGSQVGSYFLFFIIIAFLLYANVLFLKRRSSELALYQTLGLSKFNIIY
                     ILMFEQLLIFSITAILGITIGIFGSKILLMIVFTLLGIKEKVPIIFSFRAVFETLLLI
                     GVAYFLTAAQNLILVLKQSISELSKHNQPKQMNHNKITFEEVILGILGIILIISGYYY
                     SLRIVHYYDAIGVLMFILFATVIGAYLFFKSSVSLVFKIIKRFKKGVISVNDVMFSSS
                     IMYRIQKNAFSLTVMAIISAITLSVLCFAAISRATLSSEIKYTSPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDDLFDIKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGAIKDLVKHKKHGKAVIGTKKHHVDIKLRKDINKIYFMTDVDLGGPTFVLND
                     QDYQEIRKYTKPKHIVSQFGFDLKHKKDMLALEKATNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGISFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMAKGLKFKIA
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPIFIVMGLYICMYAIFAITAYNHSKRT
                     IRHSI"
     gene            6910..7527
                     /locus_tag="RJE42_001543"
     CDS             6910..7527
                     /locus_tag="RJE42_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDV5876960.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            7543..8550
                     /locus_tag="RJE42_001544"
     CDS             7543..8550
                     /locus_tag="RJE42_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDV5876961.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(9132..9929)
                     /locus_tag="RJE42_001545"
     CDS             complement(9132..9929)
                     /locus_tag="RJE42_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDV5876962.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            10290..10934
                     /locus_tag="RJE42_001546"
     CDS             10290..10934
                     /locus_tag="RJE42_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV5876963.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            11631..13781
                     /locus_tag="RJE42_001547"
     CDS             11631..13781
                     /locus_tag="RJE42_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5876964.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKFNKSIMLNPEPLVGGMAQEVKD
                     VILNYIDHYKNHLTDVIHIDEDKFEIPKLFQTVIQINTYTEMKLVFLEGLFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYYVEEAFMNVI
                     RQLSPNEINHHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFDKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIAQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHQTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSNNSLNINVTFNALYIIKIGKK"
     gene            13927..>13990
                     /locus_tag="RJE42_001548"
     CDS             13927..>13990
                     /locus_tag="RJE42_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5876965.1"
                     /translation="MNTEKLETLLGFYKQYKALSE"
BASE COUNT         5052 a         1785 c         2350 g         4803 t
ORIGIN      
        1 tagactcgaa gatttttata gtaaggaaaa ctaaataata tataaagcac cactaatcgt
       61 agttgatagt taatcaatta cacattagtg gtgttttatt ttgaatttaa atttgataaa
      121 tgaataagag tgtacgtatt tatttgagat tagataatat tgataagtgc gagcaatgta
      181 tcaaggtaac cattaaattc gttgttatac gattctaata caagattatg ataaaatagc
      241 tttaattaag aattttagcc atcatatagt catcataata tttaccatcg ataaataact
      301 tatcttttaa aacgccttcg atttgaaaat cggcactttt aaaaagctcg agggcaggtt
      361 ggttattgag tggtacattt gcttcaattc ggtgtatttg attgtttaaa caccaagcca
      421 taatggcatc aagaagtgct tggccaattc cacgatgttg atataatttc tttacaccta
      481 aatcaatttt agcaacatgt ttaatgcgtt gaaatggtgt cgtattaaca aaggcaaagc
      541 caacgagttg ttcatccctt tcagcaacaa agatgacttt atgtggagaa gtgatatatt
      601 cttctaattg tttactagcc gatgtgacgc taggatcata ttctcctggt gtatagaaca
      661 tatacggaga ttcgtcgtat attttcgcta acattgaaat gaaattttct acatctttga
      721 tactaactct acgtataata tgggccataa aaagcctcca gtattttgaa attttaaaac
      781 atttgctact attataatat atatgtaact aaaaggtgga gtaatatgtt ttttggttac
      841 agtttaaagg agtattttag atgaaaccta aagttttatt agcaggtgga acagggtata
      901 ttggtaagta tttaagtgaa gtgattgaaa atgatgctga actttttgct atatcaaaat
      961 atccagacaa taaaaaaaca gatgatgttg aaatgacttg gattcagtgt gatatatttc
     1021 attacgaaca ggttgttgcc gcaatgaatc aaatagatat tgctgtattc tttatcgatc
     1081 caacaaagaa ttctgccaaa ataacacaat catcagcaag agatttaaca ttaatcgcag
     1141 cagataattt tggtcgagca gcggctatca atcaagtaaa aaaagtaatc tacatacctg
     1201 ggagtcgtta tgataatgaa acaattgaac gcctaggtgc atatggtaca actgtagaaa
     1261 caacaaattt agtttttaaa cgttctttag ttaatgtaga attacaagtt tcaaagtatg
     1321 atgatgttag atcaacgatg aaggtagttt taccaaaggg gtggacatta aagaacgttg
     1381 taaaccattt tattgcatgg atgggttaca ctaaaggaac ctttgtaaaa acagaaaaat
     1441 cacatgatca atttaagata tatattaaga ataaggtgcg accgctcgca gtatttaaaa
     1501 tagtagaaac agctgatgga ataataactt taattttatt gagtggaagt ttagtgaaaa
     1561 aatatacagt taatcaaggg aagttagaat ttagattaat caaagagtcg gcagtcgttt
     1621 atatacatct atacgattat atccctcgat tattttggcc gatttattac tttatacaag
     1681 caccaatgca aaaaatgatg attcatggct ttgaagttga ctgccggatt aaagattttc
     1741 aaagtcgatt aaaatcagga gaaaatatga aatatactaa atgatattgg gtgatatgga
     1801 tgcaaatact actagtagaa gatgacaata ctttgtttca agaattgaaa aaagaattag
     1861 aacaatggga ttttaatgtt gctggtattg aagatttcgg caaagtaatg gatacatttg
     1921 aaagttttaa tcctgaaatt gttatattgg atgttcaatt acctaaatat gatgggtttt
     1981 attggtgcag aaaaatgaga gaagtttcca acgtaccaat attattttta tcatctcgtg
     2041 ataatccaat ggatcaagtg atgagtatgg aacttggcgc agatgattat atgcaaaagc
     2101 ctttttatac aaatgtatta attgctaaat tacaagcgat ttatcgacga gtgtatgaat
     2161 ttactgctga agaaaaacgt acattgactt ggcaagatgc tgtcgttgat ctatcaaaag
     2221 atagtataca aaaaggtgaa cagacgattt ttctatccaa aacagaaatg attatattag
     2281 aaattcttat tactaaaaaa aatcaaatcg tttcgagaga tacaattatt actgcgctat
     2341 gggatgatga agcatttgtc agtgataaca cattaacagt taatgtgaac cgattacgta
     2401 agaaactatc tgaaataggg atggacagcg caattgaaac aaaagtagga aaaggatata
     2461 tggcacatga ataatttgaa atgggtagtt tattttctga aatcaagaaa gaattggata
     2521 ttttggatac tgtttttaaa tattcttatg ttaggcatta gtcttattga ctatgacttt
     2581 ccaatcgata gtttgtttta tattgtttct ttaaatttaa gcttaacact gatttttctt
     2641 atattaactt tttttaaaga agtaaaatta tatagacatt ttgaaaaaga taaagaaatc
     2701 gaagaaatta aacataaaga tttagcggaa acgccatttc aacgtcatac agttgattat
     2761 ttatatcgtc aaattttagc gcataaagat aaagtggtcg accaacagtt gcaattaaaa
     2821 atgcatgaac aaaccatcac agaatttgta cacgacatca aaacaccagt gactgctatg
     2881 aaattgttaa ttgaccaaga agagaatcaa gaaagaaaac aagcgctatt atttgaatgg
     2941 tcacgtatta actcgatgtt agatacgcaa ttgtatatta cgagattaga gtcgcaacgt
     3001 aaagatatgt tttttgatta tgtctcgttg aagcgcatgg ttattgatga aattcaatta
     3061 acaaggcata taagtcaagt taaaggtatc ggatttgata ttgattttaa agtggataat
     3121 catgtgtaca cggatataaa atggtgtcgt atgattatta gacagatttt atcaaatgct
     3181 ttgaaataca gtgagaatta taatgttgat atttcaacgg aattaataga tcaacatgtt
     3241 gcgttaatca tcaaggatcg cggaagagga attagtaaaa aagatatgcc tcgaatattt
     3301 gaacgtgggt ttacgtcaac ggctaacaga aatgaaacga catcctccgg catgggtcta
     3361 tacttagtgg atagtgtgaa agatcaatta ggaatacaat tacaagtcac ttcgactatt
     3421 ggaaaaggaa caacggttaa attgatcttc ccattacaaa atgaaattgt tgagcgcatg
     3481 tcagaagtga caaatctgtc attttaaaca tgcgttttgt tacttagaat tgatacatca
     3541 tgaccacttc aacgatataa tcaaatagat gttaattaaa tacaaaattg aaggtaagcg
     3601 tgccaaattg aatataaagg aaatgaagtt aagataaata taggagtgtt aaagtgacaa
     3661 tattagaagt aaaacaatta acaaaaatat atggtaataa gaaaatggca caagaagttc
     3721 tgcgagatat caatatgtct gttgaagaag gtgaatttat tgctattatg ggaccttcag
     3781 gatctggtaa aacgacctta ttaaatgttt tgagttcaat tgattatatt tcgcaaggtt
     3841 ctatcacact aaaaggacac aaacttgaaa agctttcaaa taaagcatta tctgatattc
     3901 gaaagcatga tattggtttt atttttcaag agtataattt actgcataca ttaactgtta
     3961 aagaaaatat catgttacca ttaacggttc agaaattaga taaggacaca atgttagatc
     4021 gttatgaaaa agtggcagaa gcattaaata tattagacat cagtgataaa tatccatccg
     4081 aattgtctgg tggtcaaaga caacgaacat cagctgccag agcatttata acattgcctt
     4141 ctattatatt tgccgatgaa cctacaggtg cactcgattc gaaaagtaca caagatttat
     4201 taaagcgatt aatgaaaatg aatgaagaga ttaagacgac aattattatg gtgacacatg
     4261 atccggttgc agctagctat gcaaatcgag tagtgatgct taaagatggg caaatattca
     4321 cggagttata tcaaggtgat gatgacaaac atactttctt taaagaaatc atacgtgtac
     4381 aaagtgtttt aggtggcatt aattatgagt tttaatgaga ttatatttaa aaatttccgc
     4441 caaaatttat cacattatgc catttatctt ttttcgctaa ttacaagtgt agttttgttt
     4501 tttagcttcg tagcactaaa atatactcat agcattaaca taactgagac atatcccata
     4561 attaaagaag gatcgcaagt aggtagttac tttctatttt ttatcattat tgcctttttg
     4621 ctatatgcca atgtactatt tcttaaacgt cgtagttctg aacttgcatt atatcaaaca
     4681 ttaggtttat ccaaattcaa cattatatat atattgatgt ttgaacaatt attaatattt
     4741 tcaataacag ctatattggg tattacaatt ggtatctttg gttctaaaat tttattaatg
     4801 atagtgttta ctttattagg tatcaaagaa aaggttccaa ttattttcag ctttagagca
     4861 gtgtttgaga cactattatt aattggcgtt gcttatttcc taactgcagc tcaaaatctt
     4921 atcttagtat tgaaacaatc tatttcagaa ctatcaaagc ataatcagcc taagcaaatg
     4981 aatcataata aaattacatt tgaagaagtt atacttggaa ttctaggtat tattttaatc
     5041 atcagtgggt attactactc tttacgcatt gtacattact atgatgcaat tggcgtcctt
     5101 atgtttatat tgtttgcgac agtaattggg gcgtatttat tttttaaaag ttcagtatcc
     5161 ctggtattta aaattattaa aaggtttaaa aaaggtgtaa taagtgtaaa tgatgtcatg
     5221 ttttcttcat ctattatgta tcgaattcag aaaaatgcat tttcacttac tgttatggca
     5281 attatttcag ctattacttt atcagtactt tgttttgctg ctatcagtag agcaacttta
     5341 tcaagtgaaa ttaagtacac gtcacctcat gatgttacga ttaaagatca acagaaggcg
     5401 aatcagttag caagtgaatt gaacaaccaa aaaattcctc atttttataa ttataaagaa
     5461 gtgattcata caaaacttta taaggatgac ttatttgata taaaagcaaa agaaccatat
     5521 aacgtaacaa ttactagtga caaatatatt cctaatacgg atttgaaacg tggtcaggct
     5581 gatttatttg tggcggaagg cgctattaaa gatttagtga agcataagaa acatggtaag
     5641 gcagtgatag gaacgaaaaa acaccatgtg gatattaaat tacgtaaaga tatcaataaa
     5701 atttacttta tgactgatgt tgacttaggt ggaccgacgt ttgtgttaaa cgatcaagat
     5761 tatcaagaga taagaaagta tactaagcca aaacacattg tttctcaatt tggatttgat
     5821 ttaaaacata aaaaagatat gttagcattg gaaaaagcaa caaataaagt tgacaaatct
     5881 attgaaacaa gaagtgaagc cataagttcg atatcgagtt taacgggaat attgttattt
     5941 gtcacctcat ttttaggaat tagtttccta attgccgtat gttgtatcat atacattaaa
     6001 caaatagatg aaactgaaga cgagttagaa aattatagta ttcttagaaa acttggattt
     6061 acacaaaaag atatggcaaa aggattaaaa tttaaaattg cgtttaattt tgggttacca
     6121 ttagtcattg cactttcaca tgcatacttt acatcgttag catatatgaa attgatgggg
     6181 acaacgaatc aaataccgat atttatagta atgggactat atatttgtat gtatgccatt
     6241 tttgcaataa cagcttataa tcattctaag cgtacaatca gacattcaat ttaatatata
     6301 tatagcggct ttcagtaggc tagtggatta agattcatga gctttactga aagcttttat
     6361 ttttggatag ttatagatcc gggcacgata gttttaatgt atatgcttca actttgtaaa
     6421 ttgtattaaa tgtgagaaaa tagaggcata ggcattgaaa gaaatatgaa ttaagaatag
     6481 ttagaagaaa gataagtgta ttttaatatg taaaagttat gtaataaata ttgtatcgtt
     6541 gcaaattttc cgactatgtc catttacaat tattagagtt ttgcagataa aaatttgcct
     6601 atatatcaag ttttttaaaa caaatgccta ccactattta aattttatgt aaaagttaag
     6661 tttcggaatt gttaaaaatt tgtaaattga attgtgggat tgtaaatttt gtgctatttt
     6721 catataaggg caaatacagt aaattgttta ctgtgaggca tttttgaaat taatatcagt
     6781 acactaaaaa tatactgacg attgataata acaaatttgt atcattagtt tgtaaattca
     6841 cttgtcttaa tttgaaacaa attataactg aatgtgattg gtgacaatcg cttaaatgga
     6901 ggattttaaa tgtttagtaa gaaaaaagat aagtttatgg ttcaattaga agagatggtt
     6961 tttaacctag atcgtgcagc tattgaattc ggtaaaatgg atttcaatac acatctagat
     7021 ttaaaggcat actcagacaa cattaaaact tatgagtcac atggtgacga attagtacat
     7081 caagtaatta ctgatttaaa tcaaacattt atcacaccaa ttgaacgtga agatatttta
     7141 tcattatgtg atgcaattga tgatgtttta gatgcaattg aagaaacggc agctatgttt
     7201 gaaatgtatt caatcgaata cacagatgaa tatatggctg agtttgttga taacattcaa
     7261 aaagcagttg cagaaatgaa acttgctgtc ggcttattag tcgataaaaa attatcacat
     7321 atgcgtattc attcaattaa tattaaagaa tttgaaacaa actgtgatgg tattttaaga
     7381 cagtcaatta aacatatttt caatagcgaa acagatccaa tcacattaat taaaataaaa
     7441 gatatttatg aaagcatgga agaaattgct gataaatgtc aaatcgtagc aaataatttt
     7501 gaaactatta ttatgaaaaa tagctaaggg gagtatatat ttatgtcata tataatcatc
     7561 gtcactatag ctgtagttat tttctcgctg atatttgact ttatcaatgg attccatgat
     7621 acagccaatg cagtagctac tgctgtatct actagagcgt taacgcctaa aacggcaatt
     7681 ttaatggcag cagtgatgaa ctttataggt gctttaacat ttacgggcgt tgcaggcacc
     7741 attactaaag acattgtcga tccatttaaa ttggaaaatg gattagttgt tgtgttagct
     7801 gcaatacttg cggctattat ttggaattta gctacttggt tttacggaat tccaagttcg
     7861 tcttcacatg cacttatagg ttcaattgcg ggtgcagcaa tcgcatctga aggctcattt
     7921 ggcgtattac attaccaagg tttcacaaaa attattattg tattaatcgt ttcaccgatt
     7981 atcgcatttt gtgttggttt cttgatgtat tcaattttta aagttatctt taaaaatgca
     8041 aatttaacaa gagcgaatcg taactttaga tttttccaaa ttttcacagc agcgttacaa
     8101 tcattctctc acggtacgaa tgatgcgcaa aaatcaatgg gtattattac gttggcattg
     8161 attgttgcta atgtacagaa tgatggcagc gttgaaccac agttatgggt aaaatttgcc
     8221 tgtgcgacag caatggggct tggtactgca attggtggct ggaaaattat caaaactgta
     8281 ggtggtaata ttatgaaaat acgtcctgca aatggtgctg cggccgattt atcatctgca
     8341 ttaacaattt ttgttgcatc atcgttacat ttcccattat caacaactca cgtggtgtca
     8401 tcatcaatct taggtgttgg tgcttctaac cgagctaaag gtgtaaaatg gagtactgcg
     8461 caacgaatga tcattacatg ggtgattaca ttacctattt cagcattgtt agcaggttta
     8521 ttattctata tacttaactt atttttctaa ttgaaaatta agctatactg aacttcagta
     8581 tcacaaatat atggtggtat tgaagttcat tttttatgta aatagataac atattttata
     8641 gaatttacat agaatgacca agacaaagca ttttaattag gggctaagtt ataaatgtat
     8701 tccatggaac ataatcattt agttcattaa aatataagta agcgtaaaat aatgatttag
     8761 tttatattct tcaatgaatg aataattaaa tagctatcaa cttgtttaat taacctaaat
     8821 tgtattggac tttatttaat gtaaatttgt atatgtagac gaaaaagcgt aaaatactgg
     8881 tataacaaat taaatcatta tatagtcaac ttagttgcat tattttgatt tttaatatat
     8941 tatgcattta atttaatcaa ataggtaggt cataattttt acctaactac cattcataca
     9001 aaatataaat aagtatgata gggaagggtg agataatttt tgtgccaaaa tgaaaatttt
     9061 tttcaaaaat aaaaaaacta gcaatctcac atgatgtgaa ttgctagtat atatcagtac
     9121 aatttattta attaatggat gaatgcatag ctagaaactt ctgaagctgg aattgtacgg
     9181 tagttcatat tatatggacc atatgtgtaa ttcatttcag aaatcaagat actaccatca
     9241 ccattgacac gttcaacata agcaacatga ccatatggac caggtgtgct ttgcataatt
     9301 gaaccaactg atggtgtgtt gtttacttgg taaccatcat tagctgcgtt accagcccaa
     9361 tacttagcgt ctgaccaata tgtgctaatt ggactaccag cttgagcacg acggtcaaat
     9421 acgtaccatg tacattgacc agcagtgtat aaattttggt gattaaaaga tgatgcattg
     9481 ccattgctac ctgttgtagc tgttggtgtt gtaccacctg atccaccatt aggaatttgt
     9541 aatgtttggt taggcataat taaataacca cgtaagttat tggctgccat taattgatca
     9601 actgaaacac catatctgct agcaatgata tttaatgatt caccagcttg tacagtatga
     9661 gatgatgctg agccagcttg tggagaagtg tttgatgtat tttgtgcatc acttccacct
     9721 actgagataa cttgaccagg gaataccaag ttgttatcta attggttatt ttgtttaata
     9781 ctctctactg aagtgttgta tttttgagca atactccata atgattcacc agattgtact
     9841 gtatgttgtg tagatgcttg tgcatcatga tgcgttagaa atgcagctgc accagatgtt
     9901 gctgttattg caaatgctaa ttttttcaaa gggactcctc cttaaaatta agatttacta
     9961 cttttaaaaa ttattaagac aatttatata gtaaattaag ttttcgtttt atattacgct
    10021 ttccatatta tcaaaaataa gtagctttgt gtggtttgtt attatcttgt aatattatta
    10081 acattttagg atatcggttc atatttttat aaatgctgat ttgatagtgt ataaaagcgt
    10141 tttagtgatt ttaactaatt ataaaaaatt atattttgta acattgtaaa atgtaaaatt
    10201 actatacttg ttaaaggtta tttcttttga tgaaacaaaa tttactgtaa aatttgacgc
    10261 ataaaacgac agtagaagga ggaattattt tgtcgcaaaa tacaaatcat tcatattatc
    10321 atcaaaatca gcatgctcaa tcaataagta aagtgtggct ttattttatg tattattgga
    10381 ttatatttgg cataggatgc tatctaggtc agtttttacc attaagttgg cgacaaccct
    10441 tgtcatttgg attactgatt attattttag caacacttgt ttttgaaaga gcaagaaggt
    10501 tcggtttaat tatttcacat atttacgccg tagtgattgg cttattgtca tacgcaacgt
    10561 ttaccacgta tttacaaaat ttaggaccag atattttcta taaaaatatc gcattagcaa
    10621 tttttgcatt tatagcattt ggcattattg gttatttctt cgttggagat gcatcgagta
    10681 taggcaagta tttattcgtt acattaataa cattaattat tgcgagtcta attggtattt
    10741 ttcttcaaaa tcctattttt tacactatta ttaccgtcgt tagtctgttg ttatttctac
    10801 tttatacttt gtatgatttt aatcgtttaa aaagaggtga ctattcacca agagaaatgg
    10861 gatttaatct atttattaat ttgttgaata ttattaagga tatactttat cttgctaata
    10921 tgttcagaag ataaacagtt aaattaataa agaaatttgt ataaagtttt ggaataatca
    10981 ataatgaata tcaaccttaa actggagcga ttgtcataag ttagaaactt agactgagat
    11041 tttactgata aagcaataaa aatatattac cttaattgta aagatattat taatcattga
    11101 aatgattaac tgctatagtt ctaaagtaaa caaatcttag aagataaact ttaaacaggt
    11161 gtacttgcct ttctaattac acttttatta gaaaagtaaa gtatagctac gtattaattt
    11221 taaaatcgaa atattaatag gaataagtat agataatata aacaatttaa tgttagtttg
    11281 agctcatctc aaaagtagaa tgtgatttaa ataagtgttt atttttaaat tgaaaatgaa
    11341 tgtaaagcta acaaaagata atagtaatac gttgtagatg tggtcattta aaattatttt
    11401 gaaaatgcta aaaagtagtg cattttcaat tggatagttt aattaaactg actacatcta
    11461 tttttgtaat atttaaatga ttgatatata agtttaaaaa ttacccatta tatagtttta
    11521 tttatttttg atttggtatt cgtattgtgt taaatttacg tttaattaaa taagttaaat
    11581 attataggga atgataaaag tggtacttaa atatagaaag aggtaaagtt atggcaaaat
    11641 catgcttgca tatacttact aataatgaat atgcgacaac gcgttgccaa gatggcatag
    11701 tcttattttg gccaattgac ggggaaatcg aactacaaaa atttcgtaaa agtaaaataa
    11761 ttgaagatga tatatatatc attaatcatc tggatgtatt tagtattaag aataataaaa
    11821 aaacgatcat gttgtatttg agtagcgatt ggtttgcgga attaggcttt actttcttta
    11881 attatcacta tacagcaaag ttaattaaat catcctataa tttgaaatgt ttactattaa
    11941 aattgactta tcgatacctt gataatcagc ctcttaatga cgctgatatt agaaaattac
    12001 aggatattat taaaatcatt gcaaaagaag caagtatgga taaaaagatt gcgcaaaatc
    12061 aatatcgtta tgcttattat ggtgatttgc gagatgagct cgaatatatt tatcaaaatg
    12121 taaatcagcg attgacatta aaaagtgtcg ctgataaatt atttgtttca aagtcaaatc
    12181 tgtcttcgca attccactta cttatgggca tgggttttaa aaaatatatt gatactttga
    12241 aaattggtaa atccatagag attctactta ctacagatag tactattagc aatataagtg
    12301 aacatttagg atttagcagc agctcaacgt actctaaaat gtttaaaagt tatatggata
    12361 taacaccgaa tgaatatcgt aatttatcaa aatttaataa atctataatg cttaatccag
    12421 agccacttgt aggcggtatg gctcaagaag taaaagacgt catattgaat tatattgatc
    12481 attataaaaa tcacctaact gatgttatac atattgacga agacaaattt gaaataccta
    12541 agttatttca aacggttatt caaattaata cttatacaga aatgaaacta gttttcttag
    12601 aaggactttt taaaacctta ttgaataaga atagccaagt tgtctttttc atcatgccat
    12661 caattttaaa aagtaaaaat accatgtccg aagaagaaaa gtttacaatc attaaaacca
    12721 taattgaaag tgatttaaag atagcattta atataaatga tattgaaacg acttactatg
    12781 ttgaagaagc ttttatgaat gttattagac aattatctcc aaacgaaata aatcatcata
    12841 ataattacga agtgcatttt gtttttgatt tatcattgat ggaaattagg acaatttacc
    12901 gtatgatttt aaaactacat aacatcatgt taaatgtgaa attaggattg aatattacct
    12961 gtttatttga taaaccttca gtttttaaat cactagtatc acaaataaag cgacttaaat
    13021 tcgattcgtt aataatagat aatgcaaatt taagtagccc ttatttgatg ggggaaagtg
    13081 atgagttact attgaaaaat attttgcatt ttaaaaattt aaaacaagtt attaacgaat
    13141 tggatatagc acaagaaaag ctgatttttc taaatgttga aaatcataaa ctgcttaata
    13201 ataaagaacg agatttaagt aatagtgctc cattaattta taagacatta agtgcgctgt
    13261 atcataactt tgatggtttt ggattaaaca tttttgataa tcatcaaaca tttaatgcga
    13321 tgcatctata tgataaaaat ggatttaaaa caacactagg tcttatatta gaaaagttta
    13381 tcgaatatgt ctcgaaacca aaatacgaaa acagttatta ttctattttt gatatagaga
    13441 attattattg tctggttatt tatgattggc gagtgattga gagcgagaca attatgagca
    13501 attttgaaga tagtcaagtt tatattaatt tcaaaaataa tgttttaaac gataaatatc
    13561 taattgtaat agaaacgttg gatgaaaata gtgggaacat taatcatttg atctctaaag
    13621 aattaagaga taaatatgaa tggaacccca gtttactctc taaaattgac aactacctta
    13681 aaccggcaat cgagattaaa gagcataact ttagtaataa ttccttaaat attaacgtta
    13741 cttttaatgc gttatacata attaaaatag gaaaaaaata acaccttgat atgtattgca
    13801 aaatttaatt tgcatttgtt ggtaattttg acaatgtatt aaaaacaaat gagaaagaca
    13861 atggcagaaa aatttaagat aataatgaca gaagcattgt ctttatatat ttgggggtgc
    13921 aacattttga atactgagaa attagaaaca ttgcttggct tctataaaca atataaagca
    13981 ttatctgaat
//
LOCUS       DAPIDM010000061        13502 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000061 DAPIDM010000000
VERSION     DAPIDM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.061"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..600
                     /locus_tag="RJE42_001549"
     CDS             <1..600
                     /locus_tag="RJE42_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDV5876966.1"
                     /translation="SANAQYYYKQYNRMKTRERELQHQIQLTKDNIDYFSTIEQQLHH
                     ISVHDIDEIREELAEQGFMKQRKNQTKKKKAQIQLQHYVSTDGDDIYVGKNNKQNDYL
                     TNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAMLAGYFSKAGNSGQTPVDYT
                     LIKNVHKPSGAKPGFVTYDNQKTLYATPDYEHIQKMKQS"
     gene            complement(863..1264)
                     /locus_tag="RJE42_001550"
     CDS             complement(863..1264)
                     /locus_tag="RJE42_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5876967.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYAENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(1701..1913)
                     /locus_tag="RJE42_001551"
     CDS             complement(1701..1913)
                     /locus_tag="RJE42_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876968.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTINHNDDLFKHVKDILRKQGQI"
     gene            complement(1943..2554)
                     /locus_tag="RJE42_001552"
     CDS             complement(1943..2554)
                     /locus_tag="RJE42_001552"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV5876969.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(2554..3246)
                     /gene="pyrF"
                     /locus_tag="RJE42_001553"
     CDS             complement(2554..3246)
                     /gene="pyrF"
                     /locus_tag="RJE42_001553"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV5876970.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANGAGLDGVVCSPLES
                     RMLTEKLGASFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(3356..6529)
                     /gene="carB"
                     /locus_tag="RJE42_001554"
     CDS             complement(3356..6529)
                     /gene="carB"
                     /locus_tag="RJE42_001554"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV5876971.1"
                     /translation="MPKRNDIKKILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDEIA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(6522..7622)
                     /gene="carA"
                     /locus_tag="RJE42_001555"
     CDS             complement(6522..7622)
                     /gene="carA"
                     /locus_tag="RJE42_001555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV5876972.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEDVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(7624..8898)
                     /locus_tag="RJE42_001556"
     CDS             complement(7624..8898)
                     /locus_tag="RJE42_001556"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV5876973.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDCLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(8916..9797)
                     /locus_tag="RJE42_001557"
     CDS             complement(8916..9797)
                     /locus_tag="RJE42_001557"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDV5876974.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(9825..11132)
                     /locus_tag="RJE42_001558"
     CDS             complement(9825..11132)
                     /locus_tag="RJE42_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV5876975.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(11350..11877)
                     /gene="pyrR"
                     /locus_tag="RJE42_001559"
     CDS             complement(11350..11877)
                     /gene="pyrR"
                     /locus_tag="RJE42_001559"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDV5876976.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(12278..13195)
                     /locus_tag="RJE42_001560"
     CDS             complement(12278..13195)
                     /locus_tag="RJE42_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDV5876977.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(13195..>13502)
                     /gene="lspA"
                     /locus_tag="RJE42_001561"
     CDS             complement(13195..>13502)
                     /gene="lspA"
                     /locus_tag="RJE42_001561"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV5876978.1"
                     /translation="KMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAGALGN
                     FIDRVLTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEKEVK"
BASE COUNT         4197 a         2564 c         1965 g         4776 t
ORIGIN      
        1 tcagcaaatg ctcaatatta ttataaacaa tataatcgta tgaaaacgag agaacgtgaa
       61 ttacaacatc aaattcaatt gacgaaagac aatatagatt atttttcaac aatcgaacaa
      121 caattacatc atatttctgt ccatgacatt gatgaaatta gagaagaatt agcagaacaa
      181 ggctttatga aacagcgtaa aaatcaaact aagaaaaaga aagcgcagat tcaattacaa
      241 cattatgtat caactgatgg cgacgatata tatgttggca agaataacaa gcaaaatgat
      301 tatttaacaa ataaaaaagc taaaaaaact cacacatggt tacacacaaa agatattcct
      361 ggttcacatg tcgttatatt taatgatgca ccaagtgata cgacaatcaa ggaagcggct
      421 atgttagcag gatacttttc aaaagctggt aattctggac aaacacctgt tgattacaca
      481 ttaattaaaa atgtgcacaa accatcaggt gcaaagcctg ggtttgtaac atatgacaat
      541 caaaaaactt tgtatgctac acctgattat gaacatattc aaaaaatgaa acaatcataa
      601 tcaagttgtc gacaaataaa cacctcacca taagaaagga ctactttctt ttgcgaggtg
      661 tttattttta atttaatgcg ttcattgaaa cgaagtatcg tgtttttaaa taacgtatca
      721 tcatttaatt tacaaattat ctttgtgatt ttaaaaatga gtgaaactaa aatgctaatg
      781 tgtacttaaa atagaaatac ataggtaaaa cgcatgcata agaaaaagtg tttctttatc
      841 attcaacgct gtgcgaatcc ttttactcag gtaatgctaa ttgaaaactt actccaaact
      901 tatcttgaac ccaagcaaac tctctgtatg gtggcatatt cgtttttggc attaaaatgg
      961 caccttcatc ttttaatcca ttaaatagtc gttccatttc aatagtatct tttactgtaa
     1021 caaataatga aatcgcagga ttcattggta attctgtgcc actattagca tcaatcgcca
     1081 tgaatacttg tccatttaat gtaaatattg agtgttgtac agtcccagga tcaccaggtc
     1141 cattttcagc atacttagcc attgttataa tctcactatc ttcaaataag cttgtgtata
     1201 gtttaacagc ttcttcagct tggttattaa acattaaaaa tgtcgtaatt tttggaatat
     1261 ccatgctatg cctcctatag attgaacttc tataactact attatcttaa cataattaaa
     1321 atgacaaaag cgcgtcgtac cgttaggtgc acgaatgaca aatgtcttat tggcctgttg
     1381 attaaatcac atagttaacg ataaaaatta cgcaagatta aaaatatgat acatatgtta
     1441 ataaatgata cttatcatag gtattaatgt tttgacttat tgtcaaattt gattacaatt
     1501 aaaattaagt taaaggaggg acgttatgtt acattatgtg attccagtat tagtcctatt
     1561 actcgtattc ttaattggtt ggattttaat aaagttaatt atccgctaat aaatttttta
     1621 tataataaaa gctatagtgt ttgtaatatt cggatgcggt taagcagccc atgagattaa
     1681 acactatagc ttttttgttt ttaaatttgt ccttgtttac gtaatatatc ctttacatgt
     1741 ttaaataaat catcattatg atttatagtt gttttatttt ttatatgttt ttgcttcgtt
     1801 gaactttcgt cgacatcgtg actactagat agattggaat catttgaatg attgataaat
     1861 tcgttacaat gttttatctt cccatataaa tcattagatg ttttgtcatt catttgttga
     1921 attcctcctt ctagtgtcta tattatgcta agttgtctct ccattcaact aatgtttgga
     1981 tatcttcact agaaatttta ccttcatttt cagctacttc aattaattca ttgtaatcac
     2041 ttaaagtgta aaaaggtagt tgaatattgc taaatgtatc atctgctttt ttcaaaccgt
     2101 aagtaaagat agcaacaaca cctaatactt ctgcacctgc ttgttttaag gcttcaactg
     2161 ctgtgactga agatccacct gtcgaaatta aatcttctat cacaactact tttttacctt
     2221 cacttttagc accttcgatt tgattttgct taccatgact cttatttgat gaacgaacat
     2281 aattcattgg taattttaat ttttcagcta taaaagctgc atgtggaata ccagctgttg
     2341 cagtaccaga aataacttct acttcaggaa agtgttcttt aattaagtta attaaaccat
     2401 cgcggattgc gcctcgaact aaaggataac ctaacgtaac acggttatca cagtaaatcg
     2461 gtgatttaat acctgaactc catgtatata aatcatttgg tgataatgtt acagcttcaa
     2521 tatctaataa tgattttgca atttctttag ccattatact aaccaacttt ctttaatttt
     2581 atgataactt tcgactggat tgtcactttg tgtaatcggt ctaccgacta caatatgcgt
     2641 cgaaccaagc tgtcttgctt cttccggtgt cgtaatacgg tgttggtcat cttgagatgc
     2701 acctttaggt ctaatgcctg gtgttacttt taaaaatgat gcacctaact tttcagtcaa
     2761 catacgactt tcaagaggtg aacaaacaac gccatctaaa ccagctccat ttgctaactt
     2821 ggcataattt aaaacagcct cttcaatcga tgtttgtata ttttgttcgt gtcgtaacat
     2881 gtcttctgtt gttgacgtaa gctgtgttac tgcaataatt tttgtatgtt gattatgttt
     2941 tcttaatcct ttaatggcct cagacatcat ttttacgcca ccagcagcat gaacatttac
     3001 cagatcaaca ttcaatttag ctagtccttc catcgcctta ccaactgtat taggaatatc
     3061 atgcagtttt aaatctaaaa atacatcatg gcctctttct tttatctcat taattaattg
     3121 aggaccttct tggtaaaata gttccatacc tacttttacg aataatgatt catcaaacaa
     3181 atctaaaaat tgatttactt tttcttttga ttcaaaatct aatgcaataa ttggtaaatc
     3241 tttcatcata tatgtctccc tttacgttaa gaaattcatc aaaataatga ggctgtaata
     3301 taaacttctt taagtaaatg gttaattaca gccccaccga tacagtttga ttgatttaca
     3361 tttgacgcat tgtaaatgtc atactttcaa ttacattcgt taaggcatta gctgtatcta
     3421 aagatgtcaa acatggaata ccattttcaa ctgtagtacg tctaatttgg aagccatctc
     3481 tttctacttc tttaccttta gtcattgtat ttataacgat ttgaacatca ccattttgaa
     3541 tacgtgttaa taaatcattt tcgccaccaa ttttgcctac tacttctgca ggtatgtcat
     3601 actcagctaa tttattagct gttccagacg ttgctaaaat tttatagcca acttcattta
     3661 agcgttgtgc caatttaaca acctcctctt tatctttgtc actgacagtc attaatactg
     3721 taccgtggtc tttaacttct acaccactac ctgttaaccc tttgaataac gccttttcta
     3781 atgtagtatc tttccccatt acttcacctg tagacttcat ttcaggtcct aaagtaatat
     3841 caacattttt caatttatta aaactaaata ccggtgcttt cacaaataca ccctcagcat
     3901 atggttgaac cccttcttga taacccatat ctgtcagttt ttcaccaata attgctcgca
     3961 ttgccaattg tgccattgga atatcagtaa ttttacttaa gaatggtacc gttctactag
     4021 aacgtggatt tacttctaaa acatacacac catcgtgagc tataacgaat tgaatgttga
     4081 ttaagccaat gatgtttaaa cctttagcta attttatagt atagtcctca agtgttgcta
     4141 tctcgtcttc tgtcaaagtt tgtggcggat atacagcgat tgagtcacca ctatgcacac
     4201 cagcacgttc aatatgttcc atgattcctg gaataatgac cgtttctcca tcacaaatcg
     4261 catcaacttc aatttcttta ccagttaaat atctatcgac tagtaccgga tgttccggac
     4321 tcgcttttac agcctgggtc atatagtttt ctaactcttt atcattgtct acaatttcca
     4381 ttgcgcgacc acctaataca taagaaggtc tgactacaac cggatatcca atttctgcag
     4441 cattcgctaa tgcttcctca ggtgatgtag ctgttttccc ttgtggctgt ggcacgttaa
     4501 tttttcttaa tagtgcttca aattcttttc tatcttcagc acgatttaga ttttctagtg
     4561 aagtaccaag tattttaact ccatgtttag ccaatttgtc tgctaaatta atcgctgttt
     4621 gtcctccaaa ttgtacaacg acacctttag gtttttctaa attaatgata ttcatcacat
     4681 cttcttcagt taaaggttca aagtataatt tgtcagaaat tgagaagtct gttgatactg
     4741 tttctggatt gttattcaca attatcgctt cgtaccctgc tttttgaatt gcccaaacgg
     4801 cgtgaactgt cgcatagtca aattctacac cttggccgat tcgaattggt ccagagccta
     4861 atactaagat tttttctttg tcagtaacta tagattcatt ttcagtttcg tatgtaccat
     4921 aataatatgg tgttgaagat tcaaattcag ctgcgcaagt atcaaccatc ttgtaaacag
     4981 gtttaatatc attttccata cgcaattgat atacttcttc ttccgtcata ttaaagcgat
     5041 gcgctattgt tttatcacta aatccataat cttttgcata tttaagatat tctaaatcac
     5101 cttgatgttc ttttagttga tgctcaatat caataatgtt ttggaacttg tgtaagaaga
     5161 agtaatctat ctgagtcata ttatgaattt cttctaatgt tgtgcctctt ctaattgctt
     5221 cgccgatgaa aaataatcgt tcatcatctt ggtgtgaaat acgttcttta atataatcaa
     5281 gatcgaagct ttcaccattt ggtaatccta agtgatgcac accatactca agtgatcgaa
     5341 ttgcttttaa caatgattct tcgtaagttc gaccaatggc cataacttca cctgttgctt
     5401 tcatttgtgt gccaagctct cgttctcctt tttcaaattt atcaaaagga aatcttggta
     5461 tttttgaaat cacataatct aaagttggtt caaacgctgc ataagatgtt cctgtaattg
     5521 gatttaacat ttcatctaat gttagaccaa ccgcgatttt agcagctaat tttgcaatag
     5581 gatatcctgt tgcttttgaa gctaacgctg atgaacgtga tacacgcgga tttacttcta
     5641 taatataata atcgaatgaa tgcggatcta atgctaattg aacattacaa ccaccttcga
     5701 tacctaaagc tcgaataact tttaatgaaa catcacgtaa catttgatac tcaacatctg
     5761 ataatgtttg gctaggagcc acaacaattg aatcacctgt atgaatacca actggatcaa
     5821 tattttccat gttacataca acgatggcat tatcgttttt atcacgcatt acttcgtatt
     5881 cgatttcttt aaaacctgcg atagattttt ctaataaaca ttgcgttgct ggactataat
     5941 gaagaccatt tgagacgatt tcatgtaatt cttcatcatt atgacaaata ccgcctccgg
     6001 taccacccat cgtaaatgct ggtctaacaa ttagcgggta tcccacttgc tctttgaatt
     6061 taaaggcttg ctctactgta tttacaatgt cactctctgg cacaggaacg tttaaatcat
     6121 tcattaatgt tctaaacatt tcacggtctt ctgcttgttg aattgatgtt agctcagttc
     6181 ctaataattg gacgttatta tcttgaagca caccactttc gtgtagttga atcgccatgt
     6241 ttaaacctgt ttgaccacct aaagttggaa gtaaagcgtc aggttgctct ttacgtataa
     6301 ttcgcgctat aaaatcatga gttaacggtt cgatgtatac tttatccgca atttccttat
     6361 cagtcatgat tgtcgctgga tttgaattta caagaataac tcggtatccc tcttctttta
     6421 aagctagaca tgcttgtgtt ccagcataat caaattcagc tgcttgacct atgataattg
     6481 gcccagaccc tattactaaa atttttttga tatcattacg tttaggcatt gatatgacgc
     6541 tccttttcct taaaattagt catcattgct acaaaatcat caaatagata gtttgaatct
     6601 gacggtcctg gatttgcttc aggatggtat tgaacagaaa atgctggtaa tgttttatgt
     6661 tttaaacctt ctacagtacc atcatttaat gcaagatgag taacttctaa atcagtactt
     6721 tttaacgaat ctatatctat tgcatatcca tggttttgac tcgtaatatc aacttttcca
     6781 gtctctaaat ttttaactgg atggttcgca ccacgatgac caaacttcat tttaaatgag
     6841 cttgctcctt gagataatgc aaaaagttga tgtcctagac agataccaaa gaacggaatt
     6901 ttccctaaaa ttccttgaat cattggaatc gcacattcta catcttcagg attacctggt
     6961 ccgtttgata gcataacgcc atctggagcc attgctaaaa tttcttcggc agtagttgta
     7021 tatggtacaa ccgtgacgtt acaacctctg acgtttaatt ctcgaacaat attttgcttc
     7081 ttaccaaagt ctacaagtac gacacttaga tccttacctg tcgaaacata cggtgtttta
     7141 gtcgaaacga tttctacttc gtttttaggt aattctactt gttgtaaatg tttgacaagt
     7201 tgatcaatat cttcttttcg atcagtaaaa ccagctttta acacaccgtg ttgtcgaatt
     7261 ttacgcgtaa tacttcttgt atcaacacct gcaatccctg gaatttgatg caattctaaa
     7321 acgtcatgaa gtgtcttttg ttgtctaaaa ttacttggat gcgcactcgc ttctttcact
     7381 acaataccgt ttaatgtagg tactaatgat tcaaaatcgt ctctattgat accataatta
     7441 ccgattaatg gatacgtaaa agtaatgatc tgacctgtat atgatggatc tgaaatagtt
     7501 tcttgataac ctgtcatcgc tgtattaaat acaatttctc ctacagttaa gttatcagat
     7561 cctaaacggt agccttcgta aaaagaaccg tcttctaaca ctagataacg tttgctttgc
     7621 atattattta tccccctcaa atttaacttc gccttcaacc attgttaaga tcggatttcc
     7681 ataaacttta tagccgataa atggtgtatt atctgctttt gataagaaat cttcaccttt
     7741 aatttcttgt tcactatcta aatcaatgat tgttaaatct gcataaccat tttcttttaa
     7801 tgtgccgtat tctaaattaa atgtctcaca tggtttaatt gttaagcaat ctactaattg
     7861 ttgtaatgtc caatcaccat tttttacaaa atgtgtatat aataatggga atgctgtttc
     7921 actaccaaca attccgaatg gtgctttttc cattggttgt gctttttcat cacgtgcatg
     7981 tggtgcatgg tctgttgcga tacagtcaat tgtaccgtct agtaaccctt ctaacaaagc
     8041 ctctctatct tcagtacttc tcaatggtgg attcatttta taaatggcat tgttaccagg
     8101 aatatcatct tctgttaaca ataaatggtg tggtgtaact tcagctgtaa catgaatgcc
     8161 tgcgcgttta gcatcacgaa tgactctaac actttcttta gtagaaacat gacatacatg
     8221 ataatgacaa cctgctgctt cagctaatag tacatctctt gcgatttgaa cagattcaca
     8281 aatgtttgga atacctggta tacctaactc tttactgcgt ttcccttcat gcattgcacc
     8341 accatagatt aatgaattat cttcacagtg tgctacgatg gctttgttta cttttgcagc
     8401 ttcaatcatg ccttcataca tcatgcttgc agtttgtact cctacaccgt catctgtaaa
     8461 cgcaaaggca ccttctttta ctagtgctgg gaaatcaacc aattctttac ctaattgacg
     8521 tgttgtaatt gaagcataag gtaatacacg tacttgagca ttgtcatcga ttaatttttg
     8581 taaagcttca aaatgttcta cagaatctgg taccggtctt gtgttaggca ttggacatac
     8641 agttgtaaat ccacctctag cagcagcttt agtaccagtt tcaattgtct ctttatattc
     8701 accaccaggt tcgcgtaaat gaacatgaac atcgacaaat ccaggtgaca caaagtgacc
     8761 tttcgcatct atgatgtcaa caccattgct tggttcaatt gcaggtgcaa tttgtttaat
     8821 taccttacca tcaattaaaa tatctgcttg ttgtaattcg ccattttgta atactttacc
     8881 gtttttaatt aatttcatca ttttcgtccc cttacctatt ttaataattc atcaatgact
     8941 gccattctta agtaaacgcc attttccatt tgcttaaaaa ttcttgattt tgaagcttct
     9001 actaaatcgc tttgtatttc tactcctcta ttcacaggtg ccggatgcat aacaatagca
     9061 tgttcttgta atttgttata gcgcacttca tttaagccat gcttttgatg ataatcatct
     9121 gctgcaaatc tagtttcttc tgcaagccca tgtctttcat gttgaattct taataacata
     9181 actatatcta ctgtttctat aacatcatct atatttacat aaggtgcttc taaagaatca
     9241 tcaatccaag catttgggct attaaacatt acgtttgcac ctaatgcttt taaactatgg
     9301 taattactac gtgcgacacg tgaattttta atgtctccac aaatcaatac attcaagcct
     9361 tcaaaatatc catattcttc atatatcgtc attaaatcaa gcaaactttg tgttggatgt
     9421 tgtccactac catcaccagc attcgcaatt gggatgttaa tattcgctaa tttttcatag
     9481 tagttattaa acggatgtct aatgactaat aaatcacagc caatactttc taaagtttta
     9541 catgtatcat ataaagattc accttttgaa acagatgatg ttgatgtttc aaagctaatc
     9601 gtttttaacc ctagcttaag ttctgccatt tcaaaactac attttgttcg agtagaattt
     9661 tcaaagaata aatttgcgac atatttccct tcaaagtttg gtagttgacg ttcaccagat
     9721 ttaaattgac ttgccttttg gataagtttg tatatttgat ctgtagataa atgttccatt
     9781 gataataaat gattcataaa agcgccctcc ttaatttgta aatcttaatt gttttgtttt
     9841 ttctctttag gtaagattaa gttcaaaata attcctgaaa gtgcagctaa tgccattccc
     9901 tcaatttgaa ggttgatacc aatttctttt aagttaaata ctaaattacc gatacctaca
     9961 actaaaatta ctgaagctat aactaaattt cgattgttcg caaaatctac tttactttca
    10021 actagcattc ttaagccact tgctgcaata ataccgaaaa gtaatataga tactcctccc
    10081 ataactggtg taggtataga agaaattaat gcagtgaact taccaatgaa tgctaaaaca
    10141 attgctataa ctgctgcacc accaatgacg taaatactgt atattcttgt aatcgctaat
    10201 acaccgatat tttcaccata tgttgtactt ggtggtccac caataatact ggcaaacatt
    10261 gtagaaacac catcaccaat gattgattta tcaagtcctg gcttttcaaa gaagtttcta
    10321 cctacgattt tattcagtac catttggtgc ccaatatgtt cacttactgt tacgaacaca
    10381 atcggaatca ttacaagtac aagtcctaag tgaaatgaag gtacataatc tttaaatggc
    10441 agataaatat gagggaaatc tatccatttt gctgacataa ttgcatcaaa tttcacaatc
    10501 cccataaaaa tcgctaccac ataacctacg ataataccta caagtactgg aattaaagat
    10561 aggaatcctt taaaaaatcc ttgaacgact attgttacaa gcaaggttat cattgcaaca
    10621 attaagaaac tgatattgta acctttcata tctccagggt tttcatacat tgccatattg
    10681 actgcagtag gtgctaagct taaaccaatt accatgatga ctggtccaac aactactggt
    10741 ggtaataatt tcattaacca tgctgtccca cttaatttga ttagaatccc gataatgacg
    10801 tacataacac cactcatgaa taatgctaca agcatgtctc ctaagctatg cgtacttaat
    10861 cccgtgataa ttggtgtgat aaatgcaaag ctagatccca agtatgctgg tatttgcgcc
    10921 ttcgttatta agatataaag taatgtaccg attcccgaag ctagtaacgc tgcggatatt
    10981 ggtaatcctg ttaagaatgg tactagtact gttgcgccaa acatcgcaaa taaatgttgt
    11041 aagcttaaaa atgcccattg cgctggttgt ggtttttcat ttacatctag tacaggtttt
    11101 actgttcgtt caaacatttc atcattttgc ataatattca tttcctccga taataaaaaa
    11161 atctctttac atcagtatgt gtaaagagac aaaaagtgtg acaagttact acaagtcatt
    11221 ttcgtccata gaaattgact tatagttgtc gaacatgagg gtattgttag ataaacaagc
    11281 atatgaaaac ttatttatca ttcaactccc ccaccttttt cagtctctcg tactgaatta
    11341 aaaggggtat tatttaatta taactgcatt tctttgatcc atttcttcta aatagacact
    11401 taccgtttcc tctttagaag taggtatatt tttaccaaca aaatctgctc gaattggtaa
    11461 ctcacgatgt cctcgatcaa ccaaggcagc taaaccaatt ttaataggtc tagcatttag
    11521 caaaatagca tcaagtgaag cacgaaccgt tcgaccagta tacagcacat catcaataat
    11581 gatgactact ttatctgtaa tatctgtgtc gatgtctatt gcgtcttttg tcgtaagtga
    11641 tgacatgtgc tctatatcat ctctaaagta tgtaatatca attgttccag taggtatacg
    11701 ttgttgctca atttgatgaa ttttatcttg tatacgattc gctaaatatt cacctcttgt
    11761 tttgatacct aaaagaatta aattatcagt acctttgtta tactccaaaa tttcatgagc
    11821 gattctcgtt actgtacgtt gtattgcggc atcatccatt atgatacgtt cagacatatt
    11881 ctcacccttt caaaatttag aaacaaaaaa cctcatgtct ttaatatcga catgaggttt
    11941 agtatataca atcatcctat ttacatagaa gaatcgtagt attactactt attcttgcat
    12001 atgtgaaagt gttatgattc attttagtat atatctatat tttcatgtct ttgacgtctc
    12061 tctggacgct caattaaaga ctctttatgt tgtacttaaa ctgttataag gataatacta
    12121 atcaggcgct ttgtcaatct atattgaaaa attaaccatt ctcagtaaaa atgttaacct
    12181 taatctattt tataatgtat tttcatttta aaaaaacgaa aacgataaat acttatagtt
    12241 tacataataa tgttatcgta attatagcac gacacaatta tgcatctctt tttcttattg
    12301 tatctaataa atcttcaaag tcttgtggta attcagcatg tctttcaata tattcacctg
    12361 ttactggatg ttcgaatcca ataagtccag catgtagagc ttgaccacca atatccaatg
    12421 tctttttcgg tccatacttt ggatcaccaa ctaatgggaa gccaatatat ttcatgtgca
    12481 cacggatttg atgcgtacgt cctgtttcaa gttgacattc aacaagcgta taatctttaa
    12541 aatgttctag tacgttaaaa tgtgtcactg cttccttacc atcatcaaca acagccatag
    12601 attgacgatc atttttgttt ctaccaattg gcgcatcgat tgtaccgtaa tcatgaggaa
    12661 tattcccgtg aactaaagcg atatattttc ttttaacaga tttatccatt aattgttcta
    12721 caagtccacg atgagcaata tcatttttag caaccattaa taaaccagaa gtatccatat
    12781 ctatacggtg aacaatacct ggacgaattt ctccattaat acccgacaaa tttttaattt
    12841 gatacattaa accattaact aatgtattgg tataatgacc tggtgatgga tgaactacca
    12901 tgcctttcgg tttatataca actgcaacat cgtcatcttc ataataaata tctaaattta
    12961 aattttcagg tagaatatca gcttcaacca cttctttttc agtgacaact atatgatcat
    13021 taagtttcac tttataatta gatttaacaa ctttatcgtt tgcaacgact aaacctgctt
    13081 taatccaatc ttgtatctgg ttacgagacc aatcactatt taattcaggc agcaacttat
    13141 ctacacgcat acctgtttgt tctttatctg taatgttaaa ttcataagtc tccattactt
    13201 aacctccttc tcctttttat tggaagtatc ctttaataag gcaataataa ttaatattac
    13261 accaattgtt aaacttgaat ctgcgatatt aaatattgga aaatcataac caaaaatatt
    13321 tgtatcaata aagtcaacaa cttctcctgt taaaactcta tcaataaagt ttccaagtgc
    13381 acctgcaaaa agtaaactaa tagcaacttg cataaacaaa ttatattgag catctttaat
    13441 aaagaaatat actaaggcta ttaatataat aatggtaata ataaagaaaa atgtcatttt
    13501 tc
//
LOCUS       DAPIDM010000062        12873 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000062 DAPIDM010000000
VERSION     DAPIDM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.062"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..571
                     /locus_tag="RJE42_001562"
     CDS             <1..571
                     /locus_tag="RJE42_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDV5876979.1"
                     /translation="ELKKFDTENIVRFFRKLNDAEQNKMIRNLAFKYLQDYGHYVKLR
                     KNFKGKKKVYQTERASLDTKKPEDLYNDLTNKNIQSQKVYDIFISHSTKDKKTVEDII
                     RVFNEQNKTCYCDWTMDQDYLKRKYVGEYTKQVLKARMEQSKMLVLLRTENSVQSDWV
                     SFEIEYFENLKKPIYIVESLEELLEAFSD"
     gene            599..1207
                     /locus_tag="RJE42_001563"
     CDS             599..1207
                     /locus_tag="RJE42_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDV5876980.1"
                     /translation="MARKTFISYKYSEAKDLRDKIVESLGEDAKYYQGETSDSPDMSD
                     RITEYIKKELKNKIYSTSVTIVVISPNMKESNWIDWEIEYSVKQMKRGDRTSSTNGVV
                     GVVMKHNGDYSWLRNSVINSDGHTAIHTDNSYLYDIIVKNRFNQEPIEYTCDECKNID
                     ALTGSFISLVEEDEFLGNPTKYIENAYEKSKKATNYNICRRK"
     gene            1497..2279
                     /locus_tag="RJE42_001564"
     CDS             1497..2279
                     /locus_tag="RJE42_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5876981.1"
                     /translation="MLIQLDQVGRMKQGKTILKNISWQINGGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGSVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            2347..3204
                     /locus_tag="RJE42_001565"
     CDS             2347..3204
                     /locus_tag="RJE42_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDV5876982.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQIINLAKRQQIY
                     YEVFPFEGNRVSLKEDETWLRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGINQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(3334..3426)
                     /locus_tag="RJE42_001566"
     CDS             complement(3334..3426)
                     /locus_tag="RJE42_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erm Leader peptide"
                     /protein_id="HDV5876983.1"
                     /translation="MTYGSIVAILCALFAIFFIPYMEKKDKKKK"
     gene            complement(3862..4020)
                     /locus_tag="RJE42_001567"
     CDS             complement(3862..4020)
                     /locus_tag="RJE42_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV5876984.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            4700..4804
                     /locus_tag="RJE42_001568"
     CDS             4700..4804
                     /locus_tag="RJE42_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876985.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(5302..6393)
                     /locus_tag="RJE42_001569"
     CDS             complement(5302..6393)
                     /locus_tag="RJE42_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV5876986.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPGDAYLKVIALLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLVGIAITTQSIIIIVVLLIKAIITWLLLIPVFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILYVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(6659..7639)
                     /gene="czrB"
                     /locus_tag="RJE42_001570"
     CDS             complement(6659..7639)
                     /gene="czrB"
                     /locus_tag="RJE42_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDV5876987.1"
                     /translation="MSHSHHHHDHMHSHVNTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALIAFIYAEKNATATKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(7641..7961)
                     /gene="czrA"
                     /locus_tag="RJE42_001571"
     CDS             complement(7641..7961)
                     /gene="czrA"
                     /locus_tag="RJE42_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDV5876988.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            8113..8778
                     /locus_tag="RJE42_001572"
     CDS             8113..8778
                     /locus_tag="RJE42_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5876989.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFEGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            9212..10150
                     /locus_tag="RJE42_001573"
     CDS             9212..10150
                     /locus_tag="RJE42_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDV5876990.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            10181..10411
                     /locus_tag="RJE42_001574"
     CDS             10181..10411
                     /locus_tag="RJE42_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876991.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQTSASLKFSALLINVLPLCGTFVASN"
     gene            11083..12456
                     /locus_tag="RJE42_001575"
     CDS             11083..12456
                     /locus_tag="RJE42_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDV5876992.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         4642 a         1689 c         2293 g         4249 t
ORIGIN      
        1 ggaattaaaa aaatttgata cagaaaatat agtgcgtttt tttagaaaat taaatgatgc
       61 tgaacaaaat aaaatgatta gaaatttagc ctttaaatat ttacaagatt acggtcacta
      121 tgtgaaacta aggaaaaatt ttaaaggaaa gaagaaagta tatcaaactg agagagcttc
      181 tttagacact aaaaaacctg aagatttata taatgattta acaaataaga atattcaatc
      241 tcaaaaagtc tatgatattt ttatttctca tagtacaaaa gataagaaga cagttgaaga
      301 tattataaga gtttttaatg aacaaaataa aacgtgttat tgtgattgga ctatggatca
      361 agattattta aaaaggaagt atgttggtga atatacaaag caagtattga aggctcgtat
      421 ggagcaaagt aaaatgctgg ttttgttgag aacggagaat tcagttcaat ctgattgggt
      481 tagttttgaa atagaatact ttgaaaatct aaaaaaacct atatatatag tagagtctct
      541 tgaagaatta ttagaggctt tttcagatta gatatattaa aaatggaggg acttagagtt
      601 ggcgcgtaaa acatttattt catataaata ctctgaagca aaagatttaa gagataaaat
      661 agtggaatct ctaggagaag atgctaaata ttatcaagga gagacaagtg attctcctga
      721 tatgtccgat agaataacag agtatattaa aaaagaatta aaaaataaga tatattcaac
      781 ttcagtaaca attgtagtta tttcacctaa tatgaaagaa agtaattgga ttgattggga
      841 aatagaatac tcagttaaac aaatgaaaag aggagataga acatctagta ctaatggtgt
      901 tgtaggggtt gttatgaagc ataatggtga ttattcatgg ttaagaaatt ctgttattaa
      961 ttcagatggt cataccgcaa ttcatactga caatagttat ttatatgaca taatagtgaa
     1021 gaatagattt aatcaggaac ctatagaata tacgtgtgat gaatgtaaaa atatagatgc
     1081 attaacagga tcttttattt ctttagtaga agaagatgaa tttttgggta atccaactaa
     1141 atatatagaa aatgcatatg aaaaaagtaa aaaagcaacg aattataata tctgtagaag
     1201 aaaataaaaa actataacta aaattgtaag tcaattttaa ctcatgtctt aacttgttaa
     1261 gttttaatca atctttattc caaacccaca tatgttttaa aaatttaagt ttaaaaggca
     1321 tccttttaga tgatatgtct aatggggtgt tttgtttaat tatactatcg tattttgatt
     1381 tatataaaaa cgagattcta aatactttaa tttatttaaa tagtctgaca attaagagtg
     1441 atatgttaat atgattttat gagaagtatg gagtagcaat aaaggggtga cctcgcatgt
     1501 taattcaatt agatcaagtt gggcgaatga agcaaggaaa aacaatttta aaaaatattt
     1561 cttggcaaat taatggaggt gacaaatgga ttctatatgg tttaaatggt gctggtaaga
     1621 caacgctatt aaatatatta aatgcatatg agcctgcgac aactggcagt gttaatctat
     1681 ttggtaaaat gccaggcaag gtagggtatt ctgcagaaac tgtacgacaa catataggtt
     1741 ttgtatctca tagtttactg gaaaagtttc aagagggtga gagagtaatc gatgtggtga
     1801 ttagcggtgc ttttaaatca attggtgttt atcaagatat tgatgatgag gtacgtaatg
     1861 aagcacatca attacttaaa ttagttggaa tgtctgctaa agcgcaacaa tatattggtt
     1921 atttatctac cggtgaaaaa caacgagtga tgattgcgcg agctttaatg ggacaacccc
     1981 aggttttaat tttagatgag ccagcagctg gtttagactt tattgcacgc gaatcgttgt
     2041 taagtatact tgactcattg tcagattcat atccaacgct tgcgatgatt tatgtgacgc
     2101 actttattga agaaattact gggaacttta ctaaaatttt gctattaaaa gatggggaaa
     2161 gtgtacaaca agggttaata gatgatattt tgacatctga aaacatgtca cgatttttcc
     2221 agaaaaatgt agcagttcaa agatggaata atcgattttc tatggcgatg ttagagtaaa
     2281 tattttgcaa atactaacta ataatgacaa aatttaatta agctaaaatt gacagtggag
     2341 ggcaatatgg ataacgtaaa agcaatattt ttggacatgg atggtacaat tttacatgaa
     2401 aataatcaag catcaacgta tacgaaagat gtcattaatc gattgagaga gaaaggatat
     2461 aaagtatttt tggcaacagg acgttcgcat tctgaaatac atcaacttgt acctcaagat
     2521 tttgcggtaa atggcatcat tagttcaaat ggaaccattg gagaagtaga tggagaaatt
     2581 atcttcaaac atggtttatc attggctcaa gtgcaacaaa ttattaattt agctaagcgc
     2641 caacaaattt attatgaggt atttccattt gaaggtaata gagtttcttt aaaagaagat
     2701 gaaacatggt tgcgagatat gattcgtagt caagatccta ttaatggcgt aagtcatagt
     2761 gaatggtctt caagacaaga tgcgcttgct ggtaagatag attgggtaac taagtttcct
     2821 gaaggtgaat attcaaaaat ttatctattc agttctaatt tagaaaaaat aacagcattt
     2881 agagatgaat taaagcaaaa tcatgtgcaa ttacagatta gtgtttcaaa ttcatcaaga
     2941 tttaatgcgg aaacaatggc ttatcaaact gataaaggta caggcattaa agaaatgatt
     3001 gaacattttg gcatcaatca agaagaaacg ttagtcattg gtgatagtga taatgataga
     3061 gcaatgtttg aatttggaca ttacacagtc gctatgaaaa atgcacgccc tgaaatccaa
     3121 gcattaactt cagatgtaac ggcatacacg aatgaagagg atggcgcagc aaaatattta
     3181 gcaaagcatt ttttagctga ttaataacat cggcagttat ttattattta atttacaata
     3241 gttgaagagt aatttacaaa ggagcagtaa agttattttc tattagaaaa tgtcttactg
     3301 ctcctttgta tgctagtaat ccagatacta gtgttatttc ttctttttgt ctttcttctc
     3361 catatatgga atgaagaaaa tcgcaaatag ggcacagagt atagctacaa ttgaaccgta
     3421 tgtcatatta gcatcacctt ttatatggtt tatgcattta agtatactat aacaactacc
     3481 gttattttaa gtaggggtgt atttgcgtta gagcccctaa caactccaaa atcgcatttg
     3541 tgattttggg ataatcaaac atttcattcc ataagtaaga tatgtttcac tttatatgag
     3601 atttttacat tcacattctg tagtggttgt aagaaaattt tttctttctc ttaggcagta
     3661 ataggcaatt tacctgtact agtatgaact tagtagttaa atcgttcatt aagattaaaa
     3721 aacttaggaa tctcttccaa atgcaattcg tcttggtaag cattaatttc atctaatatt
     3781 tttgttattg attcaacttt atctttttct agggaggccg gaaatacaag gttttacttg
     3841 aaatttaaaa ccatcagcga attatttaaa tctacgattt attcgaccac ttgtactttc
     3901 ttttcttggc ttatacatga ctattttcaa gttatccgtt acaagttcat tatatacacc
     3961 ttctattagc tcaaacgatt gtgttaatga attaaataag gcatcacttg atttactcat
     4021 tgtcatgcat gattataaaa cgagaataat aaagtagtag cacacctata tatagtggaa
     4081 tcctttgatt atctttcgtt ttaaagttaa tgctttcttt ccagtcaaac tgtgcttgat
     4141 gatcaggttt tgtctcgaat cttgttgtat ctttaggcga catctattga taacatttca
     4201 taatataagg agtaaattca tcatgtttaa gtatataagc tctaaatgta taatatgcac
     4261 aggttaatcc atgtttttcc ttcgaatatt gccaaagtat acgcttatag taaaattttt
     4321 gttcactagt gtctgaaagt aatttttcga ggataggata atacttatca ataatagact
     4381 gacgatttct ctttcaaagt tttcattttt caatgtctat tttgaaggga gcatatcagc
     4441 cgaaaaggtt ggtgctctgc tttatcagag aataaaaaat gagaaattaa atttttatat
     4501 gatgaacaaa tgataacagg ggaagaaata gctagttaga caagggtatg tcactcaact
     4561 gtttatcgag ttgattaaga aatgaaaaaa cttatactat gaattactgt ttaaaagtgt
     4621 gcatgttata atatttattg agcaagttgg atagatggtg gctaatctct taataaaggg
     4681 gtgatgccta tggttatagt tgttactcct agaaaggact agcatgtctg attttgaaat
     4741 gctgatggtt gtattaacaa tcattggttt agtattgatt agtactcaag accataaaaa
     4801 ataaccttct attcgctttg accggcattt ttgaaggcta tttttaaata aaatataagg
     4861 tcaccgtctt tttaacgggc tcattagggt aacatgtttc cgagtgttgc cctttttgtg
     4921 tttcaagagt taaagatttt catcttttgc ttctacttac tacaaatata ttttaacaca
     4981 tttttcttat gaattgtagt tctgaacatg atcagaaata ataaagccaa ctatcaatac
     5041 aacaaaaaac aataaaaagt atagaatgta atcacgaatt atataaaaag tattggagat
     5101 ctcttcatat atagataatt cacttagtta ttttagaaag aagctcctaa caactaaagt
     5161 tgaaaaatag aggaacacag ttggatgcta tgcatcaact gcataagagc cccttacaac
     5221 taaagtttaa aagtaggagc gcctgcgcta agcgcatgca taagagcccc taacaactaa
     5281 agttgtaagg ggctctaaaa tttattttgg ttgattgtct tctggtttat ctgaagtcat
     5341 cgtttttgta gtattatcat ttaatgaatc ttgtgctttt tctgtttttg tttcaacaga
     5401 tgttgtcact ttatcatttt ctacttttgt attttttggt tcagtaacaa cttttttatt
     5461 cttgtcagct gtttttactt tatctttagt aattttttga ccacgtttta agtaatattg
     5521 aacgataccc attaaaatga ttgcatgaat aacaacgtat aaaatgattg tcattactgt
     5581 ataaactctt atgatatttt ctgcaacact ttgagagagt gattgcgtat taaatgaaat
     5641 caagtaacca acaggtgttt taaataaaat aataattaag tttaataata gtattccaat
     5701 gaaaaatttg aaaaatgttt tttgaccatt tttcattgct ttaaatccat tagctaagtg
     5761 tgtttttact ttatcgttag ttgattcaac gaaactagtc ataaagttga aaacaggtat
     5821 taacaataac caagtaataa ttgctttaat caataaaaca acaatgatta tgatgctttg
     5881 agttgtaata gcaataccaa ccaggtgtgt actatctgca tatgatgatt gtactgaatt
     5941 cataatcatt tctgatactg gagaaaataa tttgtatagt aatgatgtca gaagtgaaag
     6001 aacgataatc attgctatag atacaaggcc gatcagaacg ctctttaaat aattaccttt
     6061 tttaaatgca ataaataaat cggtaaattt tactttttca tgactcattg ctcgtttcat
     6121 aacatttgta attccgataa aaatttgaac taaaacaaac agtgaaacta cagctgctat
     6181 aagtaataga gcgataactt ttaaatatgc atctcctggt ggtttttgcc caaattgtga
     6241 ataaattgca atagtttgta catacttagc aagctggaaa ttagcaagta aatatacaac
     6301 tgcaattaca gcaaatgcta taagtgcata tattaatgtt attaataatt gtggtttagc
     6361 atttttaaat gctgatttaa agtaagtaaa caaagtggtg cctccttttc ttcatacaaa
     6421 tacaataaca aaaaacacgt tattttcata ttaatatcta ataaaaatta attaaagtga
     6481 ctaaagttag agaattataa tagtaaattg tcaaaaatat gactaatgta agaaataata
     6541 gttgaaaatt tgttaataaa cattgcatca tcaaagacca aacaagtcgc cataaagaca
     6601 taaaaaacct attgccattg aaaagtttaa tcgatgcaat ggcaataggt taaaattatt
     6661 aatgtacatg cgcgtgatga tgagcatggt ggttatgtga atgactatgt gttcctgaac
     6721 atataatcga ttcatcatgt ttgtgattag gcgtttctaa ttgaatagtc atatggtgaa
     6781 tatttaaatg taataaatca tgctcaatgt tttctaataa taattcacat tctttcattg
     6841 tcaatgtatg gtctacaaca acatgacaac ttaatgcatt catatcattt gaaattgtcc
     6901 aaacatggca atcatgcaca ctttgtattc gtgaatcctt tttaatagta gttataactt
     6961 catctatatc aacatcactt ggtgtgcctt ccattaaaat gttaattgaa gattttgtga
     7021 taccccaagc actttttaaa ataataacgg aaactaaaat acttgcaata ggatcggcga
     7081 ttgtccatcc aaatgcccaa attaaaatag ctgcagtaat ggcgccaact gaacctaata
     7141 agtctccgat aacatgtaga aaagcaccac gcatatttaa attgtgtgaa gtgtcgccgc
     7201 ctttaaacat aaagaatgca acaacgatat tgacaattaa accaataata ctaatgatta
     7261 acatttcttt agattggact tcagaaggaa caaagaaacg tttaatcgct tcaaaaacaa
     7321 tcaaaatact tattacaaaa agcgttacac cattaaataa tgctgcgagt acttcgaaac
     7381 gtttataacc aaatgttttc gtagctgtgg cgttcttttc agcatatata aatgcgatta
     7441 aagcaacacc taatgagaat gtgtcgctaa acatatggat accgtcagat agtaatgcca
     7501 agctgttagc aaggagaccg ccgatgattt cgataaacat atatagacca attattaaaa
     7561 acgatataaa caatactttc ttattatttg tatttacatg actatgcata tggtcatgat
     7621 gatgatgtga atgagacata ttataaccca ctttctttag gatgattcgc gtgatgtatg
     7681 gcttgcttta acatagttgc tacgtggatg tcatctaatg aataaatcat tgattggcct
     7741 tgtcgttttg ctttcacaag atgcacactt ttaagtaatt ttaattggtg cgagacattt
     7801 gattgagata aattcaattg atgtgaaatg tgaccaacac ttgcttcact gactgataac
     7861 aattccatga tacgtattcg attgtaatcg cctaatgcct tgaaaatttc agttacgcgt
     7921 tctaatgtat ctgtatttat ttctgaatat tgttctgaca ttatatgaca atcctttcat
     7981 atgaatattt attcatatat taatttaata cgttcccagt agtatgtcaa ttgattttta
     8041 tagttgtaac aagttttaca tatctcataa agtggtatgg catagggaat aataaatagg
     8101 agagtgttgt aaatgaatat tttggttata ggtgctaatg gcggtgtagg ttcgttacta
     8161 gtgcaacaat tagcaaaaga aaatgtacca tttactgcag gcgttaggca atccgatcaa
     8221 cttaatgcgt taaaatcaca aggtatgaaa gcaattcttg ttgacgttga aaatgattca
     8281 attgagactt taactgaaac gtttaaacca ttcgataaag ttatcttttc agtaggttct
     8341 ggagggaata cgggtgcaga taaaacaatt attgtcgatt tagatggtgc tgttaaatca
     8401 atgattgcga gtaaagaggc taatattaaa cactatgtca tggtttcaac atatgattcg
     8461 agacgtcagg cttttgatga tagcggtgat ttaaagccat atacaattgc taaacattac
     8521 gctgatgact atttaagacg ttcaggtttg aattatacaa ttttacatcc aggggcactt
     8581 acaaatgcag caggatctgg aaagatagaa gcagcacaat attttgaagg taaaggtgaa
     8641 attcctagag aagatgttgc gacagtatta aaagaaattg tcacatccaa tcattttaat
     8701 caccaagagt ttcaaattat tagtggtgac caggacatta aagatgcttt aactcaattt
     8761 gaaaatgaaa cggattaata aggatagtaa atatgatttg aagcgggtta ctttttcgta
     8821 actcgttatt tttttgaaaa tgatgaatta taagtatgaa caagtacgta ttgtttatgc
     8881 gttttacaaa gaatttgaac aatgtatggc acaactatta aagttgttta ttaattggta
     8941 taatgggcga gtaaatttaa agggaaatat gtacaaatgt aagagagata atctcgatgc
     9001 tataaatttt ttaaaatgtt aaagtactgt aaatttaagt gaagcgcttt aattggcagt
     9061 actgcaatgt tagtaaatat ggtacaatca ctaatagtta atttgaaatg tggaagttta
     9121 taatacgatg taaatgagaa caaaatcaaa atataatgga acaacaacat cagagttcta
     9181 ttgcttactt aatcacaatg gaggtgcaac aatgccatta tttttacaac caattttaaa
     9241 aacaaaatta tggggcggtc aacgtctaag tgagtttgga tatcaattag acaatgatac
     9301 aactggggaa tgttggtgtg tgtcagcaca tccaaatggt acgagcgaga ttattaatgg
     9361 accatatcaa ggtcaaacat tagaccgtat ttggtcagaa catcgtgaat tgtttggtga
     9421 tttcccaagc aaagattttc cgcttctaac taaaatagtg gatgcaagag aatcactttc
     9481 tattcatgtg caccctgata attcttatgc ttatgagcat gaaaatgggc aatatggcaa
     9541 atctgaatgt tggtatatta tagatgctga agaagatgca gaaatagtta tagggacatt
     9601 agcagagtct agagaagaag ttgcgaatca tgttcaacac ggaacgatag agtcgatact
     9661 tagatatatt aaagtaaaac ctggagaatt ctattttatt ccagcaggaa cagtacatac
     9721 tatttcttca ggaatattag catacgaaac gatgcaatcg tcagacatta catatagact
     9781 ttatgatttc aatcgtcaag ataatcaata taatgataga ccgttaaata ttgaaaaagc
     9841 tttagacgtt attcagtaca atgcaccatt acctaatatt ttgcctgaaa gtgaaattat
     9901 tgaaaaccat aagtgtacac acattgtatc gaatgatttc tttactttgg ttaaatggga
     9961 aatttctggc acgttaaatt atatgaagcc tagagagttc tgtttagtta cagtgttgga
    10021 aggtgaaggg caaatgattg tcgatggtga aattttcaaa ctgactactg gtacaaactt
    10081 tattttgact tctgaagatt tggatagtgt ctttgaaggt gatttcacat tgatgattag
    10141 ctatgtgtaa tagtgtaata acaatttagg agtgaatgat ttgaagcaat ttctatatat
    10201 tgcgttagta tgtggtgtga tagcaggtct tggtgctttc ttacatatac cgcagtatcc
    10261 gagcatgaca attccacgta tagtggctat tttaggaatt atcagtgcta tgttgacttt
    10321 taaagacaag caaaccagcg cttcattaaa gtttagcgca ttgttaatta atgtgttgcc
    10381 attatgtggt acctttgtag cttcaaatta attgtcgata gcgcgtttga atatagtaca
    10441 gtgcattaac agaagaaata tgattgtgtt aatgacattg atgaaaataa agcacatcaa
    10501 cataacctct cacttgcata gtagtgaggg ggtttttgtt ggtttatata aaatgaagta
    10561 attaatattt aatgaaaatt aagacatgct aaaattctaa aggcgtatag atatttaaaa
    10621 atgcgtatca atggcgctat agagataagc aagcatagtg gtatcatata taaatttgta
    10681 atttttattg catagtgtat gtaagcatgt tttttatatg caaaataggt agctaaatat
    10741 gttaaactgt ttgtaacaat tttatcatta agacagatat aataaataaa atatagaaaa
    10801 ctgattaaaa ggtatacagt tgctcgtatg tgtgtgtcca actgcatata gagtcgcact
    10861 tgttgtaaag caaaatagtt cgacttactt agaagtgaaa acgataaggg tctgggacgt
    10921 aaattattgt cctaggcgct acaatgttat aggaaatgcg attaaaatcg cagtgaacaa
    10981 aggggataca gttgatcgca tacgcgtcca actgcataag aaacactaaa agaatataat
    11041 tcatagtgtt tctaaaattt attcaggtta aggagtatta agatgacagc agaaacgaat
    11101 tatttttggt tgaattgtgg atataaccgt tggaatcata atgaaccgtt agtaggacaa
    11161 acagctttat ttgaatcagg cgcacatttt aacccatctc aaggatttag agcgtttaaa
    11221 aaggctaaag taggagatca agtcatcttt tatcaagttc agacggatac tgggctactt
    11281 ggatgtggtg aaattattag tgttgaaaca ggtgcacaaa ataaaatacg tgtacagttt
    11341 cgttttaatg aacaattgaa accattaaca gcagattatt taaagcgtag tgaagctttg
    11401 gaatttcgta tgagtaatat gaaagagaca ttgttcaacc aaatcactgc agaagagttt
    11461 gatttaataa gtggtcttgg taaaggcgaa attaagatac cacgttattt ctttttagct
    11521 gaaactgaag aatttgaacc tggaaaccaa tatacgattt atacgcacac gtacaacggt
    11581 attaaacgta atggttatca tttttatact cagttagaag aaggcgacaa tattattttt
    11641 tataatcgaa cgaaaaatca atctgtcgtt ggaattggag aagtatcaaa gcatattcat
    11701 gaaaaaccac caattcctgg tagaacaaat agtacagtga ttgaagtgtc ttatgagaaa
    11761 gacattacgc caattacatt aagtacatta aataagcatc ctaaattgaa aaacttatat
    11821 ttccttcaag aaaatgcaaa acaggctatt gcaagtatgt cacaagcaca gtatgatgca
    11881 attattgaaa tgagcgataa taatggattg aaatctccat ttgaaatggt tcaaaaacca
    11941 gatatgcttg aaagtgaaaa agaagaagca ttaaagccat ttattttact tgttgtagat
    12001 cgaaaagaag aaggcttaaa agcggctaat gacttattgc aaaaagcgaa tgcaaatcct
    12061 gttataacaa caggtcatcc tgatttttca gaagatatgc tttatggcaa gtatttacca
    12121 aatgaaacag gggcattata ctatcgtgaa ggatttatta ctcaattaat gcctaaaaag
    12181 gataaaagct atttagttat tgataatttt aatcgtatag atacagacat tttccaaacg
    12241 tatattaatg tgttagaagg ctatgaggta acattaccac gttataataa agacggtaat
    12301 atgattaaat ggtcacgaca aaaagattct ttctattatt tcaatcctaa ttggcatatt
    12361 gttggtatta catatgacag tttagaggaa attaaggaaa aatattcaga gcagttttta
    12421 aaatacacac gtatagtcaa agtcaaacat gactaatgat atatggtagt ttttgaaaag
    12481 aaataaggat aggattaaaa attgcataat tgcatatttg aataaccgta taaagaaaaa
    12541 atgacgggta atcaaccata agagctaatt aagagcttat gatagattac tcgtttttat
    12601 tttaataaat aataaagaca gtatatgaat ataaatgaat ttatgttact gaattgaaac
    12661 tttatttgag ttttccagtg gtatgttgaa taagataaac gactaaacgc taagttgata
    12721 ataagaaata agattacact gtagattgag aaatttaaag taaataaaaa atgcattata
    12781 caataaatac aaaactgaaa aataaatttt atggcaattt cgtgtcgcga ggacttatca
    12841 agtgacaacc ttgtttaaat gtttttaaat tag
//
LOCUS       DAPIDM010000063        12729 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000063 DAPIDM010000000
VERSION     DAPIDM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.063"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            199..762
                     /locus_tag="RJE42_001576"
     CDS             199..762
                     /locus_tag="RJE42_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV5876993.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(826..1566)
                     /gene="pmtD"
                     /locus_tag="RJE42_001577"
     CDS             complement(826..1566)
                     /gene="pmtD"
                     /locus_tag="RJE42_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDV5876994.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGDKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(1566..2438)
                     /gene="pmtC"
                     /locus_tag="RJE42_001578"
     CDS             complement(1566..2438)
                     /gene="pmtC"
                     /locus_tag="RJE42_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDV5876995.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            complement(2435..3115)
                     /gene="pmtB"
                     /locus_tag="RJE42_001579"
     CDS             complement(2435..3115)
                     /gene="pmtB"
                     /locus_tag="RJE42_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDV5876996.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPIYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFAISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            complement(3116..4012)
                     /gene="pmtA"
                     /locus_tag="RJE42_001580"
     CDS             complement(3116..4012)
                     /gene="pmtA"
                     /locus_tag="RJE42_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDV5876997.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQSGVIRVLEEDMDLNPIALKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGALEDLDEELAALLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            complement(4009..4389)
                     /locus_tag="RJE42_001581"
     CDS             complement(4009..4389)
                     /locus_tag="RJE42_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5876998.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            complement(4647..4823)
                     /locus_tag="RJE42_001582"
     CDS             complement(4647..4823)
                     /locus_tag="RJE42_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5876999.1"
                     /translation="MKHLTKIFVALAIILFIVGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNNK"
     gene            complement(5065..5163)
                     /locus_tag="RJE42_001583"
     CDS             complement(5065..5163)
                     /locus_tag="RJE42_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877000.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            5363..6649
                     /locus_tag="RJE42_001584"
     CDS             5363..6649
                     /locus_tag="RJE42_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV5877001.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFSYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSRPIVTNALTHGLSLVGDLFVNQDDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVEAIKA
                     LANKGTKVIAVVDDAYYGLFYEDVYTQSLFTALTNLHSNAILPIRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            complement(6920..8989)
                     /gene="eap"
                     /locus_tag="RJE42_001585"
                     /gene_synonym="map"
     CDS             complement(6920..8989)
                     /gene="eap"
                     /locus_tag="RJE42_001585"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDV5877002.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKQIDKSSSSLHH
                     GYSKIQIPYTITVNGTSQNILSSLTFNKNQQISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAKYTVHFKNGTKRVVDLKAGIHTADLINTSDIKAISVNVDSKKQVKDKEAKANVQ
                     VPYTITVNGTSQNILSNLTFKKNQQISYKDLENNVKSVLKSNRGITDVDLRLSKQAKF
                     TVNFKNGTKKVIDLKAGIYTANLINTGDIKNININVETKKQAKDKEAKANNQVPYSIN
                     LNGTTTNIQSNLAFSNKPWTNYKNLTAKVKSVLKSDRGVSERDLKHAKKAYYTVYFKN
                     GGKRVIHLNSNIYTANLVHAKDIKKIEVTVKTGSKAKAERYVPYSIAVNGTSTPNLSD
                     LKFKGDSRVSYSDITKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVINLNSK
                     ISQLNLLFVKDIKKIDVDVKTGSKAKADSYVPYTIAVNGTSTPIASKLKLSNKQLIGY
                     QDLNKKVKSVLKHDRGINDIELKFAKQAKYTVHFKNGKTQVVDLKSDIFTRNLFSVKD
                     IKKIDIDVKQHTKSNKALNKVANIATKVKFPVTINGFSNVVSNEFAFLHPHKITTNDL
                     NAKLRLALASDQGITKHDIGLSERTVYKVYFKDGSSKFVDLKAAKQDSKVFKATDIKK
                     VDIEIKF"
     gene            9378..10370
                     /gene="sph"
                     /locus_tag="RJE42_001586"
     CDS             9378..10370
                     /gene="sph"
                     /locus_tag="RJE42_001586"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HDV5877003.1"
                     /translation="MVKKTKSNTLKKAATLALANLLLIGALTDNSAKAESKKDDTDLK
                     LVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKK
                     EYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
                     SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPK
                     DETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPE
                     HLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK"
     gene            complement(10632..11648)
                     /locus_tag="RJE42_001587"
     CDS             complement(10632..11648)
                     /locus_tag="RJE42_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HDV5877004.1"
                     /translation="MIKQVCKNITACSLALSTALTVFPASSYAKINSEIKQVSEKNLD
                     GETKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILEPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSESISYQQPSYRTLLDQSTSNKGVGWKVEAHSINNMGHDHTRQLTND
                     SDDRVKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNKHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVRFIKVLND
                     KEQK"
     gene            complement(11670..12725)
                     /locus_tag="RJE42_001588"
     CDS             complement(11670..12725)
                     /locus_tag="RJE42_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HDV5877005.1"
                     /translation="MKNKKRVFIASSLSCALLLLSAANTEANSANKDSQDQTKKEHVD
                     KAQQKEKRNVNDKDKNTPGPDDIGKNGKVTKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEENNSSWLKYPSEYHVDFQVKSNRKTEILDQLPKNK
                     ISTAKVDSTFSYNSGGKFDSVKGVGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVVANDLKYGGEVKNRNDDFLFYRNTRLSTVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDVLKNKPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVIDKYSDENKPYKEG"
BASE COUNT         4238 a         2118 c         1753 g         4620 t
ORIGIN      
        1 aaataatata aacacaaccc agcgattttg aatacgcact gatgtcaccc tttctttgta
       61 cttatttaaa tatagcataa ggtgcttccc gtttcatcct taacaaaaat taacaaagaa
      121 tttgcaatta atttatttca ttacctttta ttactataaa ttggtataat gttgtaaatg
      181 ctaaaggagg tttttacaat gacaaattta gaaacttatt tcaaaaatag ccaaccctta
      241 aatgaataca ttgatggaat gaagacaaat caagataccg ttctcagtat ctatcaatca
      301 tttgagttac cagaagatga ttcacgcatc aaaaaaataa aagagatgaa ttattctaaa
      361 gtattagtaa ttactgaaga ctggtgtgga gatgcaatga tgaatctccc aattctaaaa
      421 cacatcagtg aagcattaaa tttagaagtg cgcgtgttcc atagagatga tgatacaaaa
      481 ctgatcgatc aatatttaac aaatggtaaa tcacgtgcaa ttcctatctt cgtattcttg
      541 aatgatcaat tcgaacaaga aacagtttgg ggaccaagag caaatgaagt ccaaaaattt
      601 gtaactgatg ttcgtgccga taaattaccg agcaaagatc atccagacta tgcagattta
      661 gaaaaagaaa cacacttagt tatatcaaat cgctataaaa ctgacgcttc attttggaaa
      721 gcagtttata attccatcct aaacaaatta gaaaataaat aaatttacaa taagacaaac
      781 tttgaggttg ggaaaattta aaattcccaa cctcaaaatt ttttattaaa tgtctttttt
      841 agcgacatat aagatattca aaatgaagaa tatcactata gaacctaatg aaattaccca
      901 ttgccaattg ctaaaatcaa agttagagct aatcatttta tcagttaagt aagcaaaagg
      961 gatataatca aaagcatcta aaactttatc accatatctt ggaataaatg taatgaatgg
     1021 tttaataaac ggtactatga gtactaaaaa tacacctaat gaaaatatca ttgctgtttt
     1081 ttgtgatgca agtgtgatta agaataaaaa caagccaaag aatagtaaaa agattaggaa
     1141 gaaccataag ttatctacat atttagaaaa cgccaaattt tttccattgc tagatgcctg
     1201 cacaacaatc aaaatcacat aatgaattaa tgcaaataat attgaaatta aaataattga
     1261 aattgttttc gaaataatgt atccaacacg tgttttaact ttactcatat atagttggat
     1321 tgtgccttgt gaaaagtcac gcgtaattgt cttaacaaca aacattaaac caattattaa
     1381 caataaccat tttgctgcag caaatacaga tccatagaca ataatatgtt tcgggttatc
     1441 catcgaattt gccataagta tactttgcgt tgcaatcaaa ctagatacga ctagtatcgc
     1501 taaatatgtt aaaggacttt taaaaatact ataaaaatca tacttaacta aatttaaaat
     1561 tctcattatt tatcacctct ttgatttata ttgaagtacg tatcacgcaa tgaactttta
     1621 cgtgtttcaa tatatttcgg ataaatatct aattcagata atgcttttag tataacttga
     1681 taattttttt gtgcattgat cattaaatgt ccatctgctt tgtcgacatt ttgtaattgg
     1741 aaatgctctg caagatattt ttcagttcta tcaaagtctt tatgatcaac agtaactatc
     1801 gttgtgtcag atgcaacacc ttcctccatg tttacatctt gaacaaaatg tccgtctctc
     1861 aaaaatacag ctctatcaca aattaattca atatcttcta atttatgact tgatattaga
     1921 attctcatat caagttcatt tactagagac ttaattgtag tcagcacatc aattgagcca
     1981 tctggatcca taccatttgt aggctcatcc aagattaaaa atttaggttt attcattaaa
     2041 gatactgcaa ttgctaactt ttgcttcatc cccattgaat atttcttaac tttcttttta
     2101 atataaggtc tcataccaaa tgcatctata attttgtctg tgtatgcttt atcaaaaccc
     2161 ttacctaata cttgtgcaaa taatttcaag ttatacaatc ctgatttatt atcatataat
     2221 ttaggatgct caattaaaaa accgatatta tctgcattat caatatctac ttttccatca
     2281 aatttaataa tattaccatt cattactttc ataacggttg ttttaccaac accgtttttc
     2341 cctattaatc cgacaattct actatcgcca aagtcaaaat caatatcatt taaaacgaca
     2401 tttgagccgt atttttttgt aatatgttct aatttcatcg actgcctcct ttaaattttc
     2461 tagtatttat tttattgagc tgtttaaaat aattaacaat aagtataaca atgcttataa
     2521 ttagaaaacc aatatttaaa atatatgaat atagatctgg gaatgatgat tgacttagaa
     2581 caaaataatt caccaacact attgcaattg aaaataaaaa tggaactaaa atgataatag
     2641 taaacacgta tataccatat ggcactttga ctctacgcaa ttctgtaaat tgactaaatg
     2701 caattggtat agacaagaag ttagatatgg caaatgcata tgcagttgaa aaatagattg
     2761 aatttggttc aatcacatct gcttcataag catataaact aataacaagc gttccaatta
     2821 atgttaaaac aatgcatgta atatagttgg cattaagcaa ttgttttttt gacacaggta
     2881 aactcatata aaaataatat gaatcattac cacctaatct acgattgagt ctaaataagt
     2941 gaccagcatc taaaatccaa ataatcaata ttgttggact tataaaagat tgcaataggt
     3001 aaaagaactt tgttgagtca agcattatat aaataggata taaggttaat aatagaacat
     3061 agatgataag tgtatatcgt cgtaatttaa aattcctaat taacaatgcc ttcatttaaa
     3121 aaccttcttc catcaatttg atgttttcat ggtatttagg ttttctcttt tcaagataaa
     3181 ccattaagtt ctcaatagaa ggtgtagtga tattcacttt atgacctaaa atttctttaa
     3241 atacttgtgc atgttctgtt aatcctataa atcccgtacg cttatgttct tcgtaaatca
     3301 gcaaagcagc caattcttca tctaaatctt cgagggctcc actcacaact tgatatcttt
     3361 gtaacagttg ttcttttgaa ccatttaaga taacttcacc gtcacttaag tgaatgatat
     3421 aatcagctat tttttctaaa tctgagataa tgtgtgtcga catgaatatt gttttatttt
     3481 catcaattaa ttcttgctga attatctcca atagttcatt tctagctaag ggatcaagac
     3541 ctgaagtagg ctcatcaaaa atatacaatt cagcatgatg tgaaaatgca actgctaatg
     3601 ataatttcat tttcatgcca gtcgaaaatg tttttattga tttattgatt ggtaaatcga
     3661 acttttcaag ataaaattca aatacttgat ggtcccattt acgataaaaa ggggcaatca
     3721 ttttttcaag ttgcttcgta gtccatctct cattaaaata attttcagcg taaacaaacc
     3781 caattctatt ctttaacgct atcggattga gatccatatc ctcttctaat actctaataa
     3841 cgccactttg tggttgatac aaatccataa ttaaccttat tattgtggtt ttaccagcgc
     3901 catttctacc aataaaacca gtgacgaatc cttgaggaac tttgaaagag atattcttta
     3961 aattaaactg atcacttgaa taattaacat tacttaattc tatggcattc atgatgattc
     4021 ctcctcataa atgaacgtta aaatatcttg aagttcctca agtgacattt cgatggcttg
     4081 cgcttcatta accaattctt taaccaaatt ttcaatggta aaaaattgtt tctcttttaa
     4141 aatagaacta tcttgctcct taacaaaggt cccttttcct ctaattgtgg taacaaaccc
     4201 atctttctct aaatcttcat aagcacgttt ggtagtaatt aaacttactt gaagatcttt
     4261 ggcaagttcc ctcattgatg gcaaatgctc tccaggagca acatgtccct ttaaaatatt
     4321 ttgttttact tgttgcttaa tctgttcata aatcggaaaa tcactattgt tttttaaaat
     4381 tattttcatc aacgtccccc tatcaccttc tgtatatata caatatacac aatatacaca
     4441 gttttgtaaa tatgcatgtt tcattatcaa ccagtcttca gaaataacac tgggtattgt
     4501 attactagca gttgaataaa atgaaatgac tctaccattt aatcttgcat tgtctacttc
     4561 aataaaaaag actgtctaaa cgaatattct tatactttaa cggtgattgt cgcgtcatta
     4621 agaacagtcg tttaaaacag tcaattctat ttgttatttt tacgccgatc attacttcta
     4681 cttataaaag cacaaatcat aaacataatt gctgccaata atagttttat accttggctc
     4741 tcatggtttg ttgcttgtaa ataataacca acaataaata aaataattgc taatgctaca
     4801 aatatttttg ttagatgttt caatttattt cacctcaaca tcgacattat aacaaatata
     4861 aagatttatc ataacttaat atattgtata gtgtaatata accaatctct cattggctta
     4921 catgacaaca aaactattca ttcaaaatga ttattgattg cgatatattg tgttcaatga
     4981 tattatttaa gtgtatccac agaattatac tacacaaccc caagtctggt accagcagac
     5041 ttggggttat ttatgtgtgt aggatcattt tttatttttc catttcagtt ctaaccaaag
     5101 ccgaaaaata attagtaacc cacctgtaat aagtgtcatc atcccttctt gccaccacat
     5161 cattacacta cacctccgtt cgactaaagt atcatactga aggatgacct acacctattc
     5221 taacacaaaa acagaacgtg tgttcgtatt ttggtagcgg attttacata ttattcgtta
     5281 attcaccaac aacactttat ctttggtata attgaagata atatataggc atacaatttt
     5341 tttaaagaga ggttaagcaa ttatgaatcc tttagcccaa agcttaaatg aacaacttca
     5401 acagtcaaat gcaactgcct ttgcaatgtt atctgactta ggtcaaaata tgttttatcc
     5461 aaagggcatt ttatcccaat ctgctgaagc aaagagtaca acatataatg caactatagg
     5521 tatggcgaca aataaagacg gaaaaatgtt tgcatcatct ttagatgcaa tgtttaatga
     5581 tttaactcca gatgaaatat tctcttatgc gccaccacaa ggcatcgaag aattacgtga
     5641 tttatggcaa caaaaaatgt tgcgtgacaa tccagagcta tcaatcgaca acatgtcacg
     5701 accaattgtt acgaatgcat taacacatgg tttatctcta gttggcgatt tatttgtaaa
     5761 tcaagatgac actatcttgt taccagagca taattggggt aattacaaac ttgttttcaa
     5821 tacgagaaat ggtgcaaacc ttcaaacata tcctatcttt gataaagacg ggcattatac
     5881 tactgattca ctagtagaag ctttacaatc atacaataaa gataaagtca ttatgatttt
     5941 aaattatcct aataatccga caggttacac acctacgcat aaagaagtga ctaccatcgt
     6001 cgaagcaatt aaagcattag ctaataaagg tacaaaagtt atagctgttg tggatgatgc
     6061 atactatggt ttattctatg aagatgtgta tactcaatca ttatttactg cattaactaa
     6121 tttacattca aatgcaatat tacctattcg tttagatggg gcaacaaaag aattttttgc
     6181 atggggattc cgtgttggat ttatgacatt tggaacgtct gatcaaacaa ctaaagaagt
     6241 tttagaagcc aaagtaaaag gtcttatacg aagcaacatt tctagtggac cgcttccaac
     6301 acaaagcgct gttaagcatg tattaaaaaa taataaacaa ttcgataaag aaatcgaaca
     6361 aaatattcaa acattaaaag aacgctatga agtcactaag gaagttgtct atgctgatca
     6421 atatcattca cattggcaag catatgactt taactctgga tactttatgg caataaaagt
     6481 tcatgatgtt gaccctgaag aacttcgtaa acatttaatt gaaaaatatt caattggtgt
     6541 tattgcacta aatgcaacag atattcgtat tgcgtttagt tgtgtagaaa aagaagatat
     6601 cccacatgtc ttcgattcaa ttgctaaagc aattgatgac ttaagataat taatttcaac
     6661 caataatctg atgagtatga cttggattat tggtttttta tttttaaaat tattccttct
     6721 ttcaaagcat cataagatat attttattca gcaacttgtc aatcgctata cctaattagt
     6781 tttatacatc tctcctaaca taacttgata tgccttagat aaatttatca aacgtgtggc
     6841 aaatcgcttc aagtatcaca tctctacaaa tgtaaaaggc accccactat tgtgagatgc
     6901 cttaatctaa aattaaagat taaaatttaa tttcaatgtc tactttttta atgtcagttg
     6961 ctttaaatac ttttgaatct tgtttcgcag cttttaagtc aacaaatttt gatgatccgt
     7021 ctttaaaata cactttatac acagtgcgtt cagaaagtcc aatatcatgt ttagtaatac
     7081 cttgatcgct tgctaacgct agtctaagtt tagcattcaa gtcgtttgtt gttattttat
     7141 gtggatgtaa aaacgcaaat tcatttgaaa ctacatttga aaatccattg attgttactg
     7201 gaaacttcac tttagtcgct atgttagcca ctttattaag cgctttatta gatttagtgt
     7261 gttgttttac atcaatatct atctttttaa tatctttgac actgaataag tttcttgtaa
     7321 agatatctga tttaaggtca acaacttgtg tctttccatt tttaaagtgt acagtatact
     7381 tcgcttgttt cgcaaatttc aattcaatat cattgatacc tctatcatgt tttaaaactg
     7441 atttaacttt tttatttaaa tcttgataac caattaattg tttattcgaa agttttagtt
     7501 ttgatgcaat aggtgttgat gtaccattta ctgcgattgt gtatggtaca tagctatccg
     7561 ctttcgcttt tgaccctgtt ttaacatcga catctatctt tttaatatct ttgacaaaaa
     7621 gcaaattcag ttggctaatt tttgagttta aattaatcac ttttttcgtt ccatttttaa
     7681 aatgtactgt ataagttgct tttttagcgt attttaattc acgttcaccg atacctctat
     7741 catgtttcaa tactgattta acttttttag tgatatcact gtagcttaca cgtgagtcac
     7801 ctttaaattt taaatcagat aaatttggtg ttgatgtacc atttactgca attgagtatg
     7861 gtacataacg ctctgctttc gcttttgatc ctgttttaac tgtaacttca attttcttaa
     7921 tatctttcgc atgaactaaa ttagctgtat aaatattcga gtttaaatgt atcactcttt
     7981 taccaccatt tttaaagtaa acagtgtaat acgctttctt tgcgtgtttc aaatcacgtt
     8041 cactaacgcc tctgtcagat ttcaatactg atttaacctt tgctgttaaa tttttgtaat
     8101 ttgtccaagg tttatttgaa aatgctaaat tagattgaat attagttgtt gtaccattta
     8161 aattaattga atatggcact tgattatttg cttttgcttc tttatctttc gcttgctttt
     8221 tagtttctac atttatattg atatttttaa tatcgcctgt attgattaag ttcgctgtat
     8281 aaatgcctgc tttcaaatcg ataacttttt tcgtgccatt tttaaaatta actgtaaatt
     8341 tcgcttgttt tgaaagtctt aaatctacat cagttatacc tctgtttgat tttaaaactg
     8401 attttacatt attctctaaa tctttataac taatttgttg attcttttta aatgttaagt
     8461 ttgataaaat gttttggctt gtaccattca cagtgattgt atacggcact tgaacatttg
     8521 cttttgcctc tttatctttc acttgctttt tagaatctac gttaacacta attgctttaa
     8581 tgtcacttgt attgattaag tcagcagtgt gaatgcctgc tttcaaatcg acaactcttt
     8641 ttgttccatt cttaaaatga accgtgtatt ttgcttgctt cgatagtctt aaatcgatat
     8701 cactaatacc tctattaaag tataaaaccg atttaacttt attctctata tctttataac
     8761 taatttgttg attcttatta aatgttaagc ttgataaaat gttttggctt gtaccattca
     8821 cagtgattgt atatggaatc tgtattttag aatatccatg atgtaatgaa cttgatgatt
     8881 tatctatttg cttagctgcg gcagatgctt cgttagtatt aaagtttgct cctgttgatg
     8941 ctaaaacacc taatgctaat gttgttgtaa tcaatgactt aaatttcata aattatctct
     9001 ccttttttat gtaattcgtg attgcaactt aattatagcc agactttctc tattttttga
     9061 attaactgaa tattaataat aaattatctt taacaataat tttttaacac tgttaaaagt
     9121 tcttttaatt ttgattaact aattaattta caatacctaa aatgttgttt gattttgttt
     9181 ataccaagct ttaaacttaa atgtcattac aaccttcatt tcttaattcc gattacattt
     9241 gtcaattata tttatagcat ctttatactc aaaaaacatt tactcaaaaa tataaattcg
     9301 atttaataat taatttaaat ttagttaatc aattttgcat ctattttgtt gtaagctata
     9361 taaaaggagt gataatgatg gtgaaaaaaa caaaatccaa cacactaaaa aaagctgcaa
     9421 cacttgcatt agcaaattta ttattaattg gggcactgac agacaatagt gccaaagccg
     9481 aatctaagaa agatgatact gatttgaagt tagtcagtca taacgtttat atgttatcga
     9541 ccgtgttgta tccgaattgg ggtcaatata agcgtgctga tttaattgga caatcttctt
     9601 acatcaaaaa taatgatgtc gtcatattta atgaagcatt tgataatggt gcatcagaca
     9661 aattattaag taatgttaaa aaagaatatc cgtaccaaac acctgtcctt ggtcgttctc
     9721 aatcaggttg ggataaaaca gaaggtagct actcatcaac tgttgctgaa gatggcggcg
     9781 tagcgattgt aagtaaatat cctattaaag aaaaaatcca acatgttttc aaaagcggtt
     9841 gtggatttga caatgacagt aataaaggct ttgtttatac aaagatagag aaaaatggta
     9901 agaacgttca cgttattggt acacatacac aatctgaaga ttcacgttgt ggtgctggac
     9961 atgatcgaaa aattagagct gaacaaatga aagaaatcag tgactttgtt aaaaagaaaa
    10021 atatcccaaa agatgaaacg gtatatatcg gtggcgactt gaatgttaat aaaggtactc
    10081 cagagttcaa agatatgctt aaaaacttga atgtaaatga tgttctatat gcaggacata
    10141 atagcacatg ggaccctcaa tcaaattcaa ttgcgaaata taattatcct aatggtaaac
    10201 cagaacattt agactatata tttacagata aagatcataa acaaccaaaa caattagtca
    10261 atgaagttgt gactgaaaaa cctaagccat gggatgtata tgcgttccca tattactacg
    10321 tttacaatga tttttcagat cattacccaa tcaaagccta tagtaaatag tgctcaacta
    10381 actaataact cgcttcgttc taaaaagtcg aagcgagttt ttattgttaa aatataaaat
    10441 gactagcttt aaactgacat cccattgttt tcatttcttt aacttccact atacctctac
    10501 atgcctcata tgcacctaac aatatcaagt taggttatcc ataataaaag atacagtgtc
    10561 tcaagctatc tatcccaaaa caaataaaaa gttacctact acgtgatacg cagcaggcaa
    10621 cttgcgaaaa cttatttctg ttctttatca ttaagtactt ttataaacct cacgttgtgt
    10681 gtcttccaat caacttcata taacgctgat aatttttctt cttttttatc tacatggttt
    10741 tcaccagacc aatagcccca gaaaccatgt ttgttccaat ctattttaaa ctcatccatt
    10801 gatcgtttat aatgtacaac aaattttgat ttaccttcat cttttttatc gtgtgacatt
    10861 acagctaaaa attctggatt aaatccttct gacacagtga caggcatttt atctttaggt
    10921 gtgaaattat ctttcgccca taagtttccg tttcgagtta atgaaaagat ttcacttttc
    10981 actctatcat cactgtcatt agttaattgt ctcgtatgat catgtcccat attattgata
    11041 gaatgtgctt ctactttcca acctacacct ttattagacg tagattggtc gagtaatgta
    11101 cgatatgaag gttgttgata actaatcgac tctgaataat tactctcttt cgtaatattt
    11161 ccagttaaac cgccacgatt aattgaaaaa tctccgcctg ttttatagcc atacgtatat
    11221 ttgacttccc ttgattcatc ttgattttta ggcgcaaagt cagtcacatt tgtattgtta
    11281 ttatcatcaa cgttttgaat agagactgaa taagagccag gccatcttaa tgtactattc
    11341 caatagccat ttggttctaa aattctcaaa ccacttccaa ttgtaccttt cgctttaata
    11401 aatactgtct ctttatcgta atttggttca gtaagaaaat taaattgtaa actttgagta
    11461 atattttttt gactatcact tgttgtagca gtacgtgtat acatttttgt ttcgccatca
    11521 agattcttct cagatacttg tttaatttca gaattaattt ttgcataaga agatgctggg
    11581 aatacagtta aagctgttga tagtgctaaa ctacatgcag taatattttt acatacttgt
    11641 ttaatcattt gtcatccgtc ccttctattt taaccttctt tgtatggttt gttttcatca
    11701 gagtatttat caataacttt aactgtttta tttttccaat caacttcata agtaacaatt
    11761 aatctttgac catctttatt tttttctaaa attggtggtg cataatgtat accaggtttg
    11821 tttttcaaaa catcttgatt tcgtgtatat gtaacttcaa attgcgtttt ctcatttgac
    11881 ttttcattag ataaataagt taaaaattct ggattaaaac cacttcgtac aagtgcagga
    11941 tatctgtatt ttgatgcaaa actcaattca ggattttcta cagtagataa tctagtattt
    12001 ctatagaata agaaatcatc atttctattt ttcacttctc caccgtactt caagtcattc
    12061 gcaacaaccg accagtgtac atgccagtta ttatttttac cgctcgcaat tgtgtcgtaa
    12121 ttttgttggt tataactaat cgtttttgaa tagctatttg atgatgttcg tccaacccct
    12181 tttaccgagt cgaatttacc acctgagtta tatgaaaatg tactgtctac tttcgcagtt
    12241 gaaattttat tttttggcag ttgatctaaa atttcagttt tacgattact tttcacttga
    12301 aaatctacat gatactcact tggatacttt agccaacttg aattattttc ttctttatga
    12361 gattcaaact ttaaatttga atgaattgac ccttgttttt tgacaagtaa gacattcttg
    12421 tcatatgttg gatcatcgat aaagtcgaat tgtaaatttt ggagtatatt tgttttctca
    12481 tcatatactg tttcagtacg ctttgtaact ttaccgtttt taccaatgtc atcaggtcct
    12541 ggtgtatttt tatctttatc attcacatta cgcttttctt tttgttgagc cttatcaaca
    12601 tgttctttct tagtttggtc ttgtgagtct ttattcgctg aattcgcttc tgtatttgct
    12661 gctgataaca ataaaagtgc acatgataat gatgacgcaa tgaatacgcg ctttttattt
    12721 ttcattgtt
//
LOCUS       DAPIDM010000064        12710 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000064 DAPIDM010000000
VERSION     DAPIDM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.064"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..170
                     /locus_tag="RJE42_001589"
     CDS             <1..170
                     /locus_tag="RJE42_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDV5877006.1"
                     /translation="TPDFERIIKANYPDYDYNHETATDPFIVDHHMRRDRSEVIAAKE
                     EAIQLRKHRRN"
     gene            complement(248..1135)
                     /gene="xerD"
                     /locus_tag="RJE42_001590"
     CDS             complement(248..1135)
                     /gene="xerD"
                     /locus_tag="RJE42_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV5877007.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(1184..1633)
                     /locus_tag="RJE42_001591"
     CDS             complement(1184..1633)
                     /locus_tag="RJE42_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDV5877008.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(1738..2280)
                     /locus_tag="RJE42_001592"
     CDS             complement(1738..2280)
                     /locus_tag="RJE42_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDV5877009.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            2362..3270
                     /locus_tag="RJE42_001593"
     CDS             2362..3270
                     /locus_tag="RJE42_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV5877010.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDFAVENGITYF
                     DTADMYDKGINESVVGKVLLKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(3486..3734)
                     /locus_tag="RJE42_001594"
     CDS             complement(3486..3734)
                     /locus_tag="RJE42_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877011.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQVITNNDESTCEVKYRKMF"
     gene            complement(3750..4505)
                     /locus_tag="RJE42_001595"
     CDS             complement(3750..4505)
                     /locus_tag="RJE42_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5877012.1"
                     /translation="MKGQHFIVTGGTSGLGFSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTLFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQME
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            4647..5462
                     /gene="proC"
                     /locus_tag="RJE42_001596"
     CDS             4647..5462
                     /gene="proC"
                     /locus_tag="RJE42_001596"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV5877013.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(5567..6487)
                     /gene="rnz"
                     /locus_tag="RJE42_001597"
     CDS             complement(5567..6487)
                     /gene="rnz"
                     /locus_tag="RJE42_001597"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDV5877014.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPIVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            6800..8284
                     /locus_tag="RJE42_001598"
     CDS             6800..8284
                     /locus_tag="RJE42_001598"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV5877015.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            8514..9380
                     /locus_tag="RJE42_001599"
     CDS             8514..9380
                     /locus_tag="RJE42_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV5877016.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(9463..11112)
                     /locus_tag="RJE42_001600"
     CDS             complement(9463..11112)
                     /locus_tag="RJE42_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV5877017.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDVKVNLKQLRPYEACVIELN"
     gene            complement(11128..12147)
                     /locus_tag="RJE42_001601"
     CDS             complement(11128..12147)
                     /locus_tag="RJE42_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV5877018.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            complement(12148..12402)
                     /locus_tag="RJE42_001602"
     CDS             complement(12148..12402)
                     /locus_tag="RJE42_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877019.1"
                     /translation="MVNVYLISKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFSESVTKLILYSGWKRFPIQRLTGGD"
     gene            complement(12341..>12710)
                     /locus_tag="RJE42_001603"
     CDS             complement(12341..>12710)
                     /locus_tag="RJE42_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5877020.1"
                     /translation="EKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEVS
                     PTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVEM
                     NGQRVFNFQDGQGNYFAVSD"
BASE COUNT         4183 a         2266 c         1902 g         4359 t
ORIGIN      
        1 taactccaga ctttgaacgc atcatcaagg ccaactatcc agattatgat tataatcacg
       61 aaacagcaac agatcctttt attgtagacc atcacatgcg gcgtgatcgt tcagaagtga
      121 ttgcagcaaa agaagaagcg attcaattaa gaaaacatag acgtaattaa ttaaccaatt
      181 taaaaaagcc acaatagcac agacattttt agtcatgtgt attgtgactt ttgagttatt
      241 gttcacttta tgctctagga tgaaattggt tatacatttt tctaatttga gattttgaaa
      301 catgtgtata gagttgagta gtagatatgt cagagtgacc taacatctct tgcactgctc
      361 ttaaatctgc gccattttcc aataaatgtg tcgcaaaaga gtggcgtaac gtatgtggcg
      421 ttaacgtctt tttaatgttt gcctttacac cattttgttt aatcattttc catattgctt
      481 gtcgtgataa aggtttacca tgcatattta aaaataagac ttcggtaaca gtctttttta
      541 aaagttgcgg tctaatcgtt tcaatataag tagttaagta ctcaatgact gcgtcgccta
      601 atggtacaat tctttcttta tcgcctttac caaatacgcg tacaaacccc attattaagt
      661 tcacgttctc taactctaaa tgtatcaatt cagatacacg cattcccgtt gcgtacagaa
      721 gttctaacat cgtacgatca cgataaccat taattttatt taaatccggc gtttctaata
      781 aagccaacac ttcgtcaaca tttaaaacgt caggcaattt tttgtcatat tttggtgaat
      841 ctaataatac cgttggatct ttcgccgcat atttttctct tatagcaaat tgatgaaaac
      901 tacggattgt tgaaataaat cgcgcaatag atttagcaga ttgcccttgg tctattaaat
      961 gccccaaaca ctcttgaatt aattgtcgat ctataaaatc aatatgcgag atatgatgtt
     1021 cagtcatata atcttgatac tttttcaaat cacgtctata agcaccaatt gtgttagaac
     1081 ttagtccttt ttcaatttgt ataaaacgca aatattcttc aataattgtt tccataacca
     1141 cacctctaaa aaaagcgaag gttaatttaa cctacgcaat ttactatcct ttacctttag
     1201 cttggcacgt ttcacacaca ccatggaaag ttaaacgatg atctaaaatt ttaaaattga
     1261 actcattttc aactcgattt tcaacttctg gtaacaaatc ttcatcgatt tcatctacac
     1321 gaccacattc catacatact aaatgatggt ggaaatgttt tgcgccttct tttcttaaat
     1381 caaaacgagc gacgccatca ccaaagttaa ttttgtcgac aacttttagt tcagctaaca
     1441 actctaacgt tctgtatact gtcgccaagc caatttcagg cgctttgtct tttactttca
     1501 gatatacgtc ttcagcactt agatgatctt tttcattttc aattagaact ctaacagtag
     1561 cttcgcgttg tggcgttagc ttatatgatg attgttgtaa ttgttgctta acgcgattta
     1621 atcgttcttc caacgatgtc cactccccta ctaataatta aaatcattac aaattatttc
     1681 aaacttctca attaaaacta acagttttct caataaaatg caagcttttc tcatttgtta
     1741 tttagaatga ttataattta ataataggtg ttgcaatgca atgatagtct tggcatcttc
     1801 tatttcttta ttcattagca tcgatttaac attctcaatc ggaactttaa tgacttcgac
     1861 aaattcatct tcatctaagt gaactgtgcc ttcttctaaa ttatccgtaa aatatattga
     1921 taattgttca tcacaaaaac ctggtgaacc atacatatct acaacatgcg ttaactcttt
     1981 ggcaatatat cctgtttctt cttctaattc acgcttagcc gcttcgactc tatcttcatc
     2041 atcttctaat ttaccagctg gaatttctag caatggtttt tctactggtt tacgatactg
     2101 tttcactaat acgacttctt ttttaggtgt caatgcacaa acagcaactg caccattatg
     2161 ataaactaat tcccttgttg acgtttcacc gtttggtaat gtcactgtat gaatttctac
     2221 atctacaatt ttgccattat aaataactgt tcgatcaatt gttttttcat ttaaatccat
     2281 tataatcacg ttcctttaaa ttcatattat atattgatac actatgcttg ttagctaaag
     2341 tgtatcgaaa ggagaacaga catgcaaaaa aatatattaa aaagtggtat tgcattatct
     2401 gaattaggtc taggttgcat gagtttaggt acagatttaa aaaaagccga acaaattata
     2461 gattttgctg ttgaaaatgg tatcacttat tttgatacag cagacatgta cgataaaggt
     2521 ataaatgaat cagttgttgg taaggtactt cttaaatatc aacaacgcga tgatattgtc
     2581 attggtacaa aagtaggcaa tcgtttaaca aaagatggca gtacaacatg ggatccgagt
     2641 aaatcctata ttaaagaggc agttaaaggt tcactaaagc gtttaggtat cgatcatatc
     2701 gatttatatc aacttcatgg tggaactatt gatgacccat tagacgaaac aataagcgca
     2761 tttgatgaat tgaaacaaga aggaattata cgtgcttacg gtatttcttc tattcgccca
     2821 aatgtaattg attattattt aaaacatagt caaatcgaaa cgataatgtc tcaattcaat
     2881 ttgattgata atcgtccaga atcattatta gatgcaatcc acaacaatga tgttaaagta
     2941 ttggcaagag gacctgtgtc taaaggatta ttaacttcaa acagtgttaa tgtgctcgac
     3001 aataaattta aagatggtat ttttgattat tctcatgatg aattgggtga aacaatagcc
     3061 tctattaaag aaattgaaag taatttatct gcattgacat ttagttattt aacatcacat
     3121 gacgtgcttg gttccatcat tgtaggtgca agtagcgtcg accaattaaa agaaaatatt
     3181 gaaaactatc atactaaagt tagtttagat cagattaaaa cagcaagagc tcgtgtaaag
     3241 gatttggaat ataccaatca tttagtgtag aagtcatttt cagtaataaa aacagcagta
     3301 tgaggcgttt cattataaaa atgccttact gctgttgttt atgtacaatt cgctataatt
     3361 tatggttaag attactcact tatgatagaa attaaagcgt tgtcctcacg catcagtatt
     3421 tagtaatttc gccctgcggc agtgccttaa gcaaacttct gccacttcat cttttaataa
     3481 ttttattaaa acatctttct atatttcact tcgcatgttg attcatcatt attagttatt
     3541 acttgtacac ccagcacatt tccttgcaac acaagtagtt tgaatttttc acaagtataa
     3601 tataatgtac tgtctgaaat ttggtctaca gaaatatcgc ctaaaatatc cagcactgta
     3661 aattcttcaa atactgatag ttgttccgca tatcgtacac aaagtcttac cacactctcc
     3721 gattgacagt tcattgccat cccacctatt tatgctttat ttttaaataa tttagggaaa
     3781 catcgttcaa aaaatctagg cgcaatttga tacattttca acgcatgatg catccattta
     3841 ggccgattaa tttccatttg ttttgtttta atgccataaa tgatatcttc tgcaagctga
     3901 ttagcatcaa gcataatttt ccccatcttt ttagcatact tcattgatgg gtcggctttt
     3961 tgatgaaaag gtgtatcaat tgggccaaca ttaactgtca tgatatgtaa gtttggtgac
     4021 tctagtctta aagcattcat taatgcataa aagcctgctt tcgatgcccc ataatgtgcg
     4081 gcatttgctt gtgtggaaaa tgcagcttga cttgaaatac ctacaatatg tgcgttagat
     4141 gttaaatatg gtctcaacac ggtatataaa acattaaaac taattaaatt tagctgatac
     4201 gtttcaatca tttctgaaaa actatggtct gaaatagatt tgaaataacc taaacctgca
     4261 ctataaatga atccatcgaa taatgtattg tcttcaaatt gcagtgcctg tatcgacttc
     4321 aaatcattta agtcacaagg aataacattt atagttttcc ccaattcctg ttcaaagatt
     4381 ctagttgctt tatcaacatc acgcaccaac aacgttacat gcactttatt ttctagtaac
     4441 tttcggacaa tcgaaaaacc taaaccactc gtaccaccag tcactataaa atgttgtcct
     4501 ttcatcaatt aaccttcctt ttcaattata tagaatgcaa tttatcaact ttacataatt
     4561 gagacaagtt gattatcttt cctaatatat atacaataat aagaaaatat aacatacaaa
     4621 tcaaaaacta aagggatgtg acgttaatga aactcgtatt ttatggagct ggtaatatgg
     4681 cacaagctat atttacagga attattaact caagcaactt agatgccaat gatatatatt
     4741 taacaaataa atctaatgaa caagctttaa aagcattcgc tgaaaaacta ggtgttaact
     4801 atagttatga tgatgcgaca ttattaaaag atgcagatta tgtattttta ggtaccaaac
     4861 cacatgactt tgatgctcta gcaacacgca tcaaaccaca tattacaaaa gacaattgct
     4921 tcatttcaat tatggcaggt attccgattg attatattaa acaacaatta gaatgccaaa
     4981 atccagttgc tagaattatg ccaaacacaa atgcgcaagt tggacactct gttactggca
     5041 ttagtttttc aaacaacttt gaccctaaat ctaaagatga aattaacgat ttagttaaag
     5101 catttggttc tgtaattgaa gtatcagaag atcatttaca tcaagtaaca gctatcaccg
     5161 gaagcggccc agcattttta tatcatgtat tcgagcaata cgttaaagct ggtacgaaac
     5221 ttggtctaga aaaagaacaa gttgaagaat ctatacgcaa ccttattata ggtacaagta
     5281 agatgattga acgttcagat ttgagcatgg ctcaattaag aaaaaatatt acctctaaag
     5341 gtggtacgac acaagctggc cttgatacat tgtcacaata tgatttagta tctattttcg
     5401 aagattgtct aaatgctgcc gtcgaccgta gtattgaact ttctaatata gaagaccaat
     5461 aaaaacaaac ccgccaacac atgtatgcat catcgcaggc actgtgtttg acgggttatt
     5521 tttataaatt attactattt ggcaagcatt gtttattact ttgtcattag actttaaaac
     5581 tatcaaaatc ttttacaaaa ttaaaatttg gtgtatcttc attttgtatc aatgtttgat
     5641 aaatttcatt tatatcttct gtattatagc gattgctcaa atgtgtaatc aacgtacgtt
     5701 taacattggc ttcttttatc aatgcaaata cgtcttcaat atggctatga tgataattgt
     5761 tggctaaatg cttttcacca tctatatagg tcgcttcatg taccatcaca tcagcatctc
     5821 tagaaatcac acgttcatta gaacatggtt ttgtatcacc aaaaattgct acaattggac
     5881 cctgtttgga ctcacctcta aaatcttttg attgataaac ttgaccatta tgttcaaatg
     5941 tatcatgaga ttttacttct tgatatttag gacctggttc aagaccaatg ttttttaacg
     6001 cttcaacatt gattgtacct gtagtttcag gtgccattac tctatatcca tatgatggaa
     6061 taccatgatt aagtaaatgc gcctctacag taaaaccatc atgatgatat gttagatgat
     6121 catcgatttc aatatatgta attggatagt ttaaatgtga ctctgataaa ttcatagaca
     6181 tttccacata tgctttaatt ccttttggtc caactaatgt aagtggcttt tgttcaccgc
     6241 cctgaaaaga acgactagaa agtaatcctg gcaaaccgaa aatatgatcg ccatgcatat
     6301 gagtaataaa tatatgtgtc acttttccta atttaattgc atgatgtaaa atttggtgct
     6361 gtgtaccttc accaacgtcg aaaagccata tggaattgga atatggttct aaatttaagg
     6421 cgattgcttg tgtatttctc tcttttgtag gcaaacctgc actcgttcca aaaaatgtaa
     6481 cttccatata tgcccctcct tttcttcaat tcattttatc ataaaatttg tatcaattta
     6541 gttttaattt atacttgaat caaaatctgt taaaaattca actttgctag ttaatttttc
     6601 taactttaac gtagacataa ctatataaat tttgataatt acgttatact tatcattaat
     6661 aagtatcaca ttaaacatga tacatgaatc gatatttcat ttaagacact gcatactgtc
     6721 gagcatattg aatgacctgc tgaatggatt atcttataat aataaatcat atatctaatt
     6781 aagaattgag gttttaatct tgagtactaa aaacaaacac atcccttgtt taatcacaat
     6841 ctttggtgca actggtgact taagccatcg taagttgttt ccatcaatat tccatctcta
     6901 ccaacaagac aatttagatg aacatattgc catcatcggt attggacgtc gtgacattac
     6961 taatgatgat ttccgtaatc aagtaaaatc atcaattcaa aagcacgtaa aagatacaaa
     7021 caaaattgac gcgtttatgg aacatgtctt ctatcataga catgatgtta gtaatgaaga
     7081 aagctatcaa gaattactag attttagtaa tgaattagat agccaatttg aactaaaagg
     7141 taatcgacta ttctatttag caatggcacc acaattcttt ggcgttattt ctgattatct
     7201 aaaatcttct ggtcttactg atacaaaagg atttaaacgc cttgttatcg aaaaaccatt
     7261 cggtagtgat ttaaaatcag ccgaagcatt aaacaatcaa attcgtaaat catttaaaga
     7321 agaagaaatt tatcgtattg accactattt aggaaaagac atggttcaaa atatcgaggt
     7381 attacgtttc gcgaatgcaa tgtttgaacc attatggaat aacaaatata tttcaaacat
     7441 ccaagttaca tcttctgaaa tactaggcgt tgaagatcgt ggtggttatt atgaatcaag
     7501 tggtgcgcta aaagatatgg tgcaaaacca catgttacaa atggttgcat tattagctat
     7561 ggaagcacct attagtttaa atagtgaaga tatccgtgct gagaaagtaa aagtacttaa
     7621 atcactgcgt catttccaat ctgaagatgt taaaaagaac tttgttcgtg gtcaatatgg
     7681 cgaaggctat atcgatggta aacaagttaa agcataccgt gatgaagatc gcgttgcaga
     7741 tgactctaac acacctacct ttgtttcagg taaattaaca attgataact ttagatgggc
     7801 tggcgtacca ttctatattc gtactggtaa acgtatgaaa tctaaaacaa ttcaagttgt
     7861 cgttgaattt aaagaagtac caatgaactt atactatgaa actgataaac tgttagattc
     7921 aaacctatta gtaatcaata ttcaacctaa tgaaggtgta tctttacatc taaatgctaa
     7981 gaaaaataca caaggtatcg aaactgaacc tgttcaattg tcttattcaa tgagtgctca
     8041 agataaaatg aatactgtag atgcatatga aaatctatta ttcgattgtc ttaaaggtga
     8101 tgccactaac tttacgcact gggaagaatt aaaatcaaca tggaaatttg ttgatgcaat
     8161 tcaagatgaa tggaatatgg ttgatccaga attccctaac tatgaatcag gtactaatgg
     8221 tccattagaa agtgatttac tacttgctcg tgatggtaac cattggtggg acgatattca
     8281 ataattgaat taaaacgcac atgttaaaca aaaataaatg agcgaatgac aaggattgat
     8341 tacgaatcaa gtaaatcaat tgttctatca ttcgcttttt ttctagacta tattatgaaa
     8401 ttatatttta caatgcccaa aactatttta ataatcattg aacaaatggg tgtataattt
     8461 atagaaataa tgtagaataa aaataaatga ttgaattaat tggagtgaaa gttttggacg
     8521 ttatcaagca aatacaacag gcaattgttt atattgaaga tcgtttatta gagcctttca
     8581 atttgcaaga attaagtgat tacgttggtc tttcgccata ccatcttgat caatcattta
     8641 aaatgattgt cggcttatct ccagaagctt atgcacgcgc gcgtaaaatg acactcgctg
     8701 caaatgatgt gataaatggt gctacacgac ttgtagatat cgctaaaaaa tatcactatg
     8761 caaattcaaa tgattttgca aatgatttta gtgattttca cggcgtatca cctattcaag
     8821 cttctactaa aaaagatgaa ttacaaattc aagagcgatt atatatcaaa ttatcaacta
     8881 ctgagagagc accctatcca tacagattag aagagacaga tgatatttca ttggttggat
     8941 atgcacgatt tatagacact aagtatttgt cacatccttt taatgttccg gattttttag
     9001 aagacttgct cattgatggt aaaattaaag agttacgacg atataatgac gttagtccat
     9061 ttgaactatt tgttattagt tgtcctcttg aaaatggttt agaaatattt gtaggtgtac
     9121 caagtgaacg ttatcctgca cacttagaaa gtcgattttt acctggcaaa cattgtgcga
     9181 aattcaattt acaaggtgaa attgattatg caactaatga agcttggtac tatattgaat
     9241 caagtttgca gttaacattg ccatatgaac gaaatgattt atatgttgaa gtgtaccctc
     9301 tcgatatttc atttaatgac ccattcacta aaattcagct ttggattcct gttaaacaga
     9361 gtccttatga cgaagattaa ataattaaaa acaaagaagc cccctaatat atctataggt
     9421 ctacaaatgg ccttagattc tattaggggg catattaata tgttaattta gttcaataac
     9481 acatgcttca tatggacgta actgttttaa attaactttg acatcataat taaatagctt
     9541 cacttctcca tgacttaaat caaatggtac agttaattct gcttcgtggt tagtaagatt
     9601 acctacaata agaacttgct tttcatttaa tgttctcgtg tatgcaaaaa cttgtgaatt
     9661 ttcagcatct actaaatcaa attgaccata tacgtataca tcattagact ttcttaattg
     9721 aattaaatct ttataaaatt gtaatactga atgctcatct tctaattgtt gtgcaacatt
     9781 gatagtttta taattcggat tcactgggaa ccacggttca ccatttgtaa atcctccatt
     9841 taacgtatca tcccattgca ttggtgtgcg agaattatct cggttctcat ctttatattt
     9901 cgcaagtaaa gcgtctacat ctccaccttg agctttcaca atttgatagt catttttaac
     9961 agcaacatcg ttaaatgttt caatactttc aaatggataa ttcgtcatac caatttcttg
    10021 accttgataa atgaatggcg taccttgttg caagaaataa actgctgcat gacttgttgc
    10081 tgattcatac caatacttgt catcgtcacc ccacgtcgat acacgtcgtg gttggtcgtg
    10141 attttcaata aataacgcat tccaaccttt attttcaagt tgtttttgcc atctatttaa
    10201 tacagattta tacgaattta catcaaagtg agaatcacca ctattccaca gtcccaaatg
    10261 ttcaaattgg aatatcatat taaatttacc attttcttcc ccgacccagt catcagcatc
    10321 atcagggctt acaccattcg cttcaccaac agtcataatg tcatacttac ttaatgagcg
    10381 atctttcatt tcttgtaacc aagtttgtat acctggctga ttcatatcta catcaaatgc
    10441 tggggcatat gttttaccct caggtacagg taagtcaccc gcttcaaacg tcttcttaat
    10501 atgcgtaatt gcatctactc taaatccatc aatgccttta tcaaaccacc agttcatcat
    10561 ttcaaataca gcatctctaa cttccggatt accccaattc aaatcaggtt gttttttact
    10621 gaatagatgg aaataatatt gctcagtatt agcatcatat tcccatgtag atccattaaa
    10681 gatactttcc cagttgttag gttcagagcc atctggcttt ggatcttgcc aaatgtacca
    10741 atcacgttta ggattgtctt tactagattt ggattctata aaccaaggat gttcatcaga
    10801 tgtatgattt acaactaaat ctaaaataag cttcatacct ctatcatgaa caccttttaa
    10861 taaacgatca aagtcttcca tcgttccaaa ttcatccata atctcttggt agtcactaat
    10921 atcataacca ttatcatcat taggtgattt aaacattgga ctgagccaaa tgacatcgat
    10981 acctaaatct tttaagtagt ccaatttatc aatcattcca ggtaaatccc caataccatc
    11041 gtgattacta tcattaaaac ttcttggata tacttgatat gctactgctt ctttccacca
    11101 ttgcttattc attttaaaac tcctttgcta tcgttgtgtt gattttctta tttttaattc
    11161 tgtatctata atgacgagtt caataacatc ctgtgctttg tttttcaata tatttaaaat
    11221 tgctgcacca gcctgttgac ctaacattcg aggcttgatg tcaatactgg tttgaggtgg
    11281 tgacgcaatt tcggttaaat aagaatcatt gaacgttgct gtcattacat ctttcggaat
    11341 ttcaatatta agttcatata ggacacttaa aatcgctaaa tgtaacatag catctaacga
    11401 aatgattgcc tgtttaatat ttgggtcctt caaacgcgta tgcagatttt gcatgtaatt
    11461 aaaaataact tctctttcat tactagtctc aataatttga taatccagat taaattgtga
    11521 tgcaactgtt tcaaatcctt gaatcctgtc tttcgaaact tcaaaatcgc ctttttctgt
    11581 aataaatatt aattcatcta caccttgttc aataacatgt cgtgtcaaat tttcagaagc
    11641 taatatatta tcattatcaa tgtgtgtaaa ttgatgatct atatccgatg taggcttacc
    11701 aatcacaata aatggcatgc tttcatcaat taacatttgt ttaatcggat cattttcttt
    11761 tgaatagagc agtataaacg catcaaccat tcgttgttta atcattttat aaacttcatc
    11821 cattaaatca ttcatattat ttgagactgt cgtctgtgta ccatagccat gctggttaca
    11881 cgtttcagaa attcctagca atacattgat gtagaatgga ttcagtcgaa taggctcctc
    11941 agaccctttt aacactaaac caattttata tgtttggtta gtaattaaag ttctagcagc
    12001 ggtattaggg aaataattca attcttccat aactttcttc acttttgaaa ttgtcgcttc
    12061 gctaatacgt tgatttcctt ttataactct tgaaactgtc gaaggagaaa caccggcttt
    12121 tagtgcaaca tctttaatcg taaccattta atcacctcct gttaatctct gtattggaaa
    12181 acgcttccaa ccactgtata atatcagttt agtcacactt tctgaaaaag tcaaaagatt
    12241 tgtgcaaacg attgcataaa acgataaaaa taaaaccttc atactgaaat tcaatccgaa
    12301 aatcaatata aaggtttgta taaatattaa aatcgattgt ttagtcacta actgcaaaat
    12361 agttaccttg gccatcttgg aaattaaata cacgttgacc attcatttct actatatcat
    12421 gcccagttaa acctaaatca tttaattttg agtataatgc atcaaagttt ttctctttaa
    12481 acattaaaga tggtgttcct aggttcacct ccgggctata cttttcaata aattcttttg
    12541 ccataatcgt caatgacgtt tcagcatctt tcgtaggtga tacttcaact gcaacatagt
    12601 cctcagctaa cggtgtttca cttacaacaa caaattctaa agtttctgtc caaaatgctt
    12661 tcgctttttc aacatcatca acatataaca taacttgatt taacttttcc
//
LOCUS       DAPIDM010000065        12677 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000065 DAPIDM010000000
VERSION     DAPIDM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.065"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            28..288
                     /locus_tag="RJE42_001604"
     CDS             28..288
                     /locus_tag="RJE42_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDV5877021.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            292..720
                     /gene="ruvX"
                     /locus_tag="RJE42_001605"
     CDS             292..720
                     /gene="ruvX"
                     /locus_tag="RJE42_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV5877022.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            735..1043
                     /locus_tag="RJE42_001606"
     CDS             735..1043
                     /locus_tag="RJE42_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDV5877023.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            1328..1966
                     /locus_tag="RJE42_001607"
     CDS             1328..1966
                     /locus_tag="RJE42_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDV5877024.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            1969..2892
                     /locus_tag="RJE42_001608"
     CDS             1969..2892
                     /locus_tag="RJE42_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV5877025.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDYRPFDEGFLYKQTVY"
     gene            2904..4172
                     /locus_tag="RJE42_001609"
     CDS             2904..4172
                     /locus_tag="RJE42_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV5877026.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            4172..4795
                     /gene="udk"
                     /locus_tag="RJE42_001610"
     CDS             4172..4795
                     /gene="udk"
                     /locus_tag="RJE42_001610"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV5877027.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            4823..5299
                     /gene="greA"
                     /locus_tag="RJE42_001611"
     CDS             4823..5299
                     /gene="greA"
                     /locus_tag="RJE42_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV5877028.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            5624..6358
                     /gene="pxpB"
                     /locus_tag="RJE42_001612"
     CDS             5624..6358
                     /gene="pxpB"
                     /locus_tag="RJE42_001612"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV5877029.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENSELNKFNEKNVHAVNQTNRIIKIPVKYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDDNFNVDDWVVIKNVN"
     gene            6348..7358
                     /locus_tag="RJE42_001613"
     CDS             6348..7358
                     /locus_tag="RJE42_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDV5877030.1"
                     /translation="MSIKILQPGLFSTVQDLARKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHSYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKAPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            7360..7809
                     /locus_tag="RJE42_001614"
     CDS             7360..7809
                     /locus_tag="RJE42_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDV5877031.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKFKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKVNDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQALVRIK"
     gene            7823..9184
                     /locus_tag="RJE42_001615"
     CDS             7823..9184
                     /locus_tag="RJE42_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV5877032.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDNAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            9184..9936
                     /gene="pxpA"
                     /locus_tag="RJE42_001616"
     CDS             9184..9936
                     /gene="pxpA"
                     /locus_tag="RJE42_001616"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV5877033.1"
                     /translation="MRIDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNEEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            9948..11180
                     /locus_tag="RJE42_001617"
     CDS             9948..11180
                     /locus_tag="RJE42_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDV5877034.1"
                     /translation="MGRNLKLKHRESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQT
                     AVFTSQFLASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISIL
                     IAFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMI
                     LGIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAH
                     RILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVF
                     EHAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVIST
                     MIFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAV
                     LVTILTGIFSFKEVLQLF"
     gene            11468..>12677
                     /locus_tag="RJE42_001618"
     CDS             11468..>12677
                     /locus_tag="RJE42_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDV5877035.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYSKRFNSLASFNTNINIIPMENG
                     KKVNDVEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKFGNYLVKIKSRPT
                     IYEISDEFLNS"
BASE COUNT         4636 a         1674 c         2455 g         3912 t
ORIGIN      
        1 atatttgtaa ttaaaggagt gtcgcaaatg gaaaactttg ataaaacaat gaaattcgac
       61 tatgaagaac ttccaacaca agatgtaaga gatgttttaa ataatgttta tcgaacatta
      121 gatgaacgag gatataatgc cgtaaaccaa attgtaggtt atttattatc aggtgaccct
      181 gcgtatattc cacgccaaaa tgaagcaaga aatcaaattc gtcatattga tagagatgtt
      241 attatggaag agcttgtttc ttactattta aaagagcaaa ataaataatt tatgttacaa
      301 cataaaattt taggactcga tgtcggtagt agaacggtag gaattgcaat tagtgatata
      361 atgggttgga cggcacaagg attagacaca ctccgaatca atgaagaaaa taatgaatta
      421 ggtattgacc aattagtaga cattattaaa aaacataatg tgggtacagt cgtaatagga
      481 ctacctaaaa acatgaataa ttcaatagga tttcgaggcg aagcttcgtt aacatataaa
      541 gaaaaattat tagaagctta tccttctatt gagattgtga tgtgggatga aagattaagc
      601 acaatggctg ctgaacgatc attattagaa gcagatgttt caagacaaaa aagaaaacaa
      661 gtgattgata aaatggcagc ggtatttatt ttacaaggct atttagattc actacattaa
      721 aaggagattt tactatgact gaacataatc atgattcaca actagaaatt aataacgaag
      781 aagaattatt aactttattc gatgaagaag gaaatgaagt tttataccga aaagttttag
      841 aattttatca tcctgaattc aaaaaagagt atgttatctt agctgaagaa ggtgctcaat
      901 cagatgaaga cgatatgatt gagcttgtac caatgatcaa tgaaccagat gagtcaggtg
      961 acggtggtaa gttagtacca atcgaaactg atgaagaatg ggacatgatt gaagaagttg
     1021 taaatactga aatggaagaa taattaaaaa gattttactc ttgctaacct taagatgggt
     1081 aggcaagagt atttttttaa gtcaagcaat gtataaataa ttaacgctta taattttatt
     1141 gaaaatatgg taaaatatct aaagttaaat tttatatgaa tgattcgctt ctataaataa
     1201 aatagctttg cgtctaacac gaaatcagtc atttaattgg ttggcaaaat tataatcaat
     1261 tgtgaaggct gatttatttt attgtcataa tcatattgac ttattttaca taaaggaagt
     1321 ttaaaacatg gatgacctaa ataaaaaata tttaatagat ttacatcaac atcaaaatag
     1381 ttcaatcgaa gttttgcgtg aatttgccga agtaaatgaa gtgccaatcg tagatcgttt
     1441 aacattagat ttaattaagc aattaattcg tatgaataat gttaaaaata ttttagaaat
     1501 tggtacagca atcggctata gttctatgca attcgcttct atatctgatg acattcatgt
     1561 cacaacgata gagcgtaatg aaacgatgat tcaatatgct aaacaaaatt tagctactta
     1621 tcattttgaa aaccaggttc gaattattga aggtaatgct ttagagcaat ttgaaaatgt
     1681 aaatgacaaa gtttatgata tgatatttat tgatgcagca aaagcgcaat caaagaaatt
     1741 ttttgaaata tatacaccac ttttaaagca ccaaggtctc gtaattacag ataatgtttt
     1801 atatcacggt tttgtatcgg atattgggat tgttagatcg agaaatgtaa gacaaatggt
     1861 taaaaaggtt caagattata atgagtggtt aataaagcaa ccaggatata caacaaattt
     1921 tttaaatata gacgatggat tagcgatttc aattaaagga gaatgactat gacagaatta
     1981 cttgtcacac ccaagtcatt aagccatatg gaaacactta ttgatttagg tgcagatgct
     2041 tttgtaattg gcgagcaaaa gtttggttta agacttccag gggaattcaa tagacaacaa
     2101 atgacagagg ctgtcgcatt agctcacaaa aacgataaaa aagtatatgc ggcggtgaat
     2161 ggattatttc ataattatca tttagatgct gtagaagatt atattaattt tttacatgaa
     2221 atacgagttg acagaattat tttcggggat ccagcagtag taatgtatgt gaaggcgcaa
     2281 gagaatccaa taccacttca ttggaatgca gagacgttag taacgaatca ttttcaatgt
     2341 aattactggg gtaaaagagg cgcatcaaga gctgtcttag cacgtgagct gaatttggat
     2401 gaaattatta atataaaaga aaattcaaat gtggaaattg aggtgcaagt tcaaggaatg
     2461 acttgtatgt tccaatcaaa gcgtatgcta ctaggcaatt attatacgtt ccaagatcgc
     2521 caaatgaaaa ttgaacgtcg caatgatgaa caatctttat tactttatga tgaagaaaga
     2581 caaaataatt acccagttta cgaagattac aatggtacgc acattatgtc accaaacgac
     2641 atttgtttaa ttgaagaatt agcaccattt tttgaggcgg gtatagattc atttaaaatc
     2701 gatggtattc tacaaacaga agaatatatt aatgtggtaa cggaacagta tcgtcaggcg
     2761 atagatttgt acaatgaaga tcctgaaatc tatgaagatg agaaatttat gttgatggac
     2821 ccaattgaag aaattcaacc tgattatcga ccatttgacg aaggtttctt atataaacaa
     2881 acagtatatt aaggaggtta atcatgaaga caatagaaga gattaaatca actcctaaaa
     2941 cagttatgaa gaaaccagaa ttattagcac ctgctggaaa cttagaaaag ttaaaaatag
     3001 cagtacatta tggcgctgat gccgtatttt taggtggtca agaatatgga ttacgttcaa
     3061 atgctgataa tttcacgatg gaagaaatag ctgaaggtgt tgaatttgcg aaccgttacg
     3121 gtgccaaaat ttatgttacg acaaatatta ttgcacatga tgagaatatt gaaggtctag
     3181 aatcatattt gcgtaatttg gaaaagacag gtgcgacagg tatcattgtt gcagatcctt
     3241 taattatcga aacatgtaaa gaagttgcgc caaaacttga aattcattta tctactcaac
     3301 aatcactttc taattacaaa gctgtagaat attggaaaga agaaggattg gatcgtgttg
     3361 tattagcacg tgagaccggc gcgatggaaa tgcgtgaaat gaaggaaaaa gtagatattg
     3421 aaatcgaagc atttattcat ggtgctatgt gtatcgccta ttcaggtaga tgtacattaa
     3481 gtaatcatat gactgcaagg gattccaaca gaggcggttg ctgtcaaagt tgtcgttggg
     3541 attatgaatt attagaagtt gatgataatg gtgaacttga tgtattttat aatcaaggtg
     3601 aagttacacc gtttgcgatg agtcctaaag atttaaaatt aatcgaatct attcctcaaa
     3661 tgatggatat tggtgtggac tcattaaaaa ttgaaggacg tatgaagtca attcattata
     3721 ttgcaacagt tgtctcagta tatcgtaaag tcattgatgc gtatgcggca gatcctgata
     3781 actttaagat taatccggaa tggttaattg agttagataa atgtgcaaat agagacactg
     3841 caccagcatt ctttgaggga acacctggtt atgaagaaca gatgtttggt caacaacaat
     3901 ctaaaaaatc accatttgat ttttgtggtt tggtattaga ttataatgaa gatacaaaga
     3961 ttgcgactat tcaacaacga aataacttta aaccaggtca agaaatagaa ttctttggtc
     4021 cagaaattga aacatttaca caagtagtag aagcaattta tgatgaagaa ggtaacagct
     4081 tagacgcggc gcgccatcca ttacaaatcg ttcaaattaa agtggatcgc ccgatatatc
     4141 caaacaacat gatgagaaag gaaattggct aatgaaagct actacaatca ttggcatagc
     4201 tggtggatct ggctcaggaa aaacaactgt aactaacgaa attatgaaaa acttagaagg
     4261 tcatagtgtc gcattacttg ctcaagatta ctattataaa gatcaaaagc acttgacttt
     4321 cgacgagcgc ctagaaacca attatgacca tccatttgca ttcgataatg atttattaat
     4381 tgaaaatctt aaagacttga aaaatggtaa agcagtagaa gtaccgacat atgattatgc
     4441 tagtcataca agaagtgaca ttaccattga ttttaaacct aaagatgtta ttatcgtaga
     4501 aggtattttc gctttagaaa ataaggtatt acgtgatatg atggatgtta aaatatatgt
     4561 tgatacagat gcagacttga gaatattacg tcgtttaaca cgagatacta aagagcgtgg
     4621 gcgttcaatg gactctgtta tcaatcaata tttaagtgtt gttagaccta tgcatgacca
     4681 atttattgaa ccgactaaga aatatgctga tataattatt cctgaaggtg ggagcaataa
     4741 agttgcaata gatattatga caacgaaaat tcagtcttta gttagcaagc aatagtataa
     4801 ttcagtaaag gaagatgaca atatggaaaa tcaaaagcaa tatccaatga ctcaagaagg
     4861 ttttgaaaaa ttagagcgtg aacttgaaga attaaaaaca gttaagcgtc ctgaagttgt
     4921 agagaaaatt aaagttgcac gttcatttgg tgacttatca gagaactctg agtatgatgc
     4981 agcaaaagat gaacaaggat tcatcgaaca agatattcaa agaattgagc atatgctaag
     5041 aaatgcatta atcattgaag atactggaga taacaacgtt gttaaaattg gtaaaacagt
     5101 aacgtttgta gaattaccag gtgatgaaga ggaaagttat caaatcgttg gttcagctga
     5161 atcagatgca tttaatggaa agatttcaaa tgaatcacca atggctaaag ctttaattgg
     5221 taaaggttta gatgatgaag ttcgtgttcc actacctaat ggtggcgaaa tgaacgtaaa
     5281 aattgttaat atccaataaa tgattgaata gattgtttaa ttgcgcgatt gaagtatgtt
     5341 aatgatactt cggtcgtgct tttttatatt caaaatcaaa aaatcggaca gatgaaaagt
     5401 aaaaattttc catcagaccg attgatagca gaacctaaat tagtgtaaga gtttcaatat
     5461 taactactcc atttaaaaaa tcaaaatatt tccgagaaaa caaattgatt ataatttgtt
     5521 attgtatgcg atttgaatta cttattaaac aacttgtgct tctcaatgat acaatgaaaa
     5581 ggtattcaat actggtttat tgcaatacaa agggggattt agagtggatg taagatttat
     5641 taacgagcag acaattatga tttattttga aaataaaata tccgaagaga catatcgaaa
     5701 tgtcacagca atggtacgat ggatacgaga aaaagaaata ttagaaattc aagatattgt
     5761 cccttcgtat cgagcagttc taatatattt tgatgaacaa gctataacat cttctaaatt
     5821 aattgaaaat tcagaactta acaaatttaa tgaaaaaaat gttcatgctg tgaatcaaac
     5881 caatcgaatt attaaaatac ctgttaaata tggtggtaca tatggacctg atattgaaga
     5941 agtagcaaag cataatcgaa taactgttga gcaagttatt gaaaaacata caagtaaacc
     6001 ttatttaata tatatgctag gatttatgcc tggatttcca tacttaggcg gacttgatga
     6061 acaactacat acacctagac gtaatcagcc aaggttaaaa attcatgcag gttctgtagg
     6121 tattgcgaat aatcaaacag gactatatcc atcagattct cctggcggat ggcaaattat
     6181 tggacgcaca ccgttaaaag tatttagcag cgagcgtgaa ccaatgtcaa tgtacgaagc
     6241 aggagaatgg atacaatttt atgcaataga tgaacaggaa tttatacaaa tcgaaagaga
     6301 tatatcagat gacaacttta atgttgatga ctgggtggtg attaaaaatg tcaattaaga
     6361 ttttacaacc aggtctcttt tcaacggtac aagatctagc aagaaaaggt tatgaacata
     6421 ttggattttc aggcgcaggt gccatggatc aatttagttt taaggttgcg cagtcattaa
     6481 ttaacaatga tggtccggcg attgaatata ctttgattgg tcctaccatt caatttaata
     6541 cgcaaaatac atttgttata accggtggta gtgttaatgc ctcgctaaat aataaaacta
     6601 tttcaatgaa ttctgtcata ttagctgaga aaggtgacat tttaaaaata ggtgctataa
     6661 ccaaaggtgc acgcggttat cttacttttg gtcattctat caacgtacct cctattgctg
     6721 aaagttatgc aacacatacc cgaagcagta ttggaggatt caaaggtaga aagttattag
     6781 ctaatgatgt aataacagtg aaaataaata atgactttaa agaaaacatt ggaaagacta
     6841 ttcatttaca ggacgattta ttgccagaaa ataatattat acacattctt caaggacctc
     6901 aattcgaggc attttctgaa gaggctagag cgaaaattgt aaatcactca tatttaatca
     6961 ctgaacaatc agaccgtatg gggtatcgat tggaaggtga cagcgttgca ccaattaatc
     7021 aagcagatat catttctgaa ccggttgctc ttggcagtgt tcaagtacca aatgatggtc
     7081 aacctattat tctgcttaat gataaacaaa cgataggtgg ttataccaaa attgcaactg
     7141 tatgcaaatt tgatcttcca aagttagcac agatgaaacc acaagataca attcaattta
     7201 aatggatatc attccaagaa gctgtagata agaatcggga acaaatgtct ctgtttaatg
     7261 aaattcttaa gagtcatcaa aaggctccaa tttttgatac atctagcttg cgacatactt
     7321 cgaaaaaatt agcaacaatt ttaaaagggg atttgtaaaa tgaatattga aaaaatcgaa
     7381 caaataatca aattagtgaa ggaaaatgat gttaagaaat ttaaatttaa aaattttgaa
     7441 gatgaaatag aaattgactt cactgactcg aatcatttgg ctgcacacag taatcaatca
     7501 aatcaaagta tgaacaataa tgatttgaca gcttcaaaag tgaatgataa ctccgatgtt
     7561 tcgacaaatg attatcatga cattaaatca ccaatggtag gtacattctt tttacaagac
     7621 agtaaagaat taactgaacc aattgtgaaa gtcggtgaca aagttaacaa aggagatatt
     7681 ataggatatg ttgaagcgat gaaagtatta aacgaggtaa caacagatgt tgctggagaa
     7741 atcactgaaa tagtagctga tcatggcaca aatgttgaat acgaccaagc tttggtacgt
     7801 attaagtaaa gggggtaagt aaatgcttcg ttgtttaatt gcgaacagag gtgaaattgc
     7861 tgtaaggatt ataagagctt gcagagaata tgggattgaa actgtagcag tttatgcaaa
     7921 aggggacgag caaagcttgc atgtacattt agcagatcaa gctatatgta ttggagaagc
     7981 taatgcttta gatagttatt taaatattga ccgcatcata tctgctgcac aaatcactgg
     8041 tgctaatgca attcacccag gatatggctt tttatcagaa tcaacaaaat ttgctcaaac
     8101 cgttgaggaa caaggcatag cgtttatagg gcctactaaa aaaacgatgg aaatgatggg
     8161 ggataaaata acagcaagac aaacagttca tcatgcaggt gttcctgtga ttccaggatc
     8221 aaatggtgct gtgaatcatg tgtctgaaat tgagaagctt gccaaagaca tcggttatcc
     8281 agttgttatc aaagctgcca gtggcggtgg tggtaaaggt atacgtattg taaagaaagc
     8341 tgaagattta gaaaaggcat ttaaagaagc taaaagtgaa ggaaaaaaat actttgatga
     8401 tgatcgtgtt tatgttgagg cttttatacc tgttgcaaaa catgttgaag ttcaagttat
     8461 gggggatggg caggataatt atgtacattt aggggaacgt gattgttctg tacaacgtaa
     8521 aaatcaaaaa ttaatcgaag aatctccttg tgcagcatta actgaagaaa gacgacaaca
     8581 aatatgtaac gatgctgtta aagttgcacg tgcagcaaat tatagaagtg caggtacgat
     8641 tgaattttta gtaactgata acgcacatta ctttattgaa atgaatgctc gaattcaagt
     8701 tgaacatact gttacagaga tgcgagcaga acgtgatttg gttgcagcac aattgtattt
     8761 attggaacat aatcatttac ctttttcaca aagcgatatt caatttaatg gtcatgtgat
     8821 agaagcgaga ataaatgctg aaaatcctga aaagaaattt caaccaacac cagggaaagt
     8881 gacagcgtta catttaccac aaggctttaa tgtgcgagtc gattctttac tatatcacgg
     8941 ttatcaagtt tcaccatatt atgattcttt agtagctaaa gtaattgtaa aatctcatga
     9001 tagagcttca gcaattgata aattgaaagc caccttagat gaaatggtga tagatggatt
     9061 ttctacaact gccgattttt tatatgcagt tttaaattat ccactatatc gtgatggaga
     9121 tgcaaaagat gttgatatta aatttttaga aaaacatcaa attgttaaag gggtggaatt
     9181 ataatgcgaa ttgatttgaa ttgtgattta ggagaagcat ttggaaatta ttcctttggt
     9241 ggtgatcatc aaattattcc gttaattact agtgcgaatg ttgcttgtgg ttttcacgct
     9301 ggtgatgaaa atgtaatgaa tgaaacggta aaacttgcca aagcacataa tgttgcagta
     9361 ggtgcacatc ctggtttacc tgatttgaaa ggctttggca gacgaaatat agatatctct
     9421 aacgaggaga tttataattt gatgatttat caattaggtg cattacaagg gttttgtcgc
     9481 attcatcaag ttaaaattaa tcatgttaaa ccgcatggtg cattgtatca gatgggtgca
     9541 aaagacagag aaatagcaag cgttatagca caagctgttt atgactttga tccatcacta
     9601 gtgttagtag gattagcaaa ttcatatcta atttcagaag caaagaatgt cggattaatt
     9661 acagcttctg aagtgtttgc tgatagacga tacgaagatg atgggcagct cgttagtaga
     9721 aaagaaagtg atgctgtgat tactgatact gacgaagcac ttaagcaggt tttgaagatg
     9781 gtgaaggaaa ataaagttat ttcaaaaaac aataaggaag taacgttaca agcagataca
     9841 atttgtgtgc atggtgatgg agaacatgca ttattatttg tttcgaaaat tagagaaatt
     9901 ttaatgaaag aaggcattga tattcaatcc ttataggggg ctaaaaaatg gggagaaatt
     9961 taaagcttaa acatcgtgaa agtgattttg aatttactaa aaaccataaa aggttattat
    10021 taggttctgt atttctgatg gcaacttctg caattgggcc agcattttta acgcaaacag
    10081 cagtatttac atcacaattt ttggcgagtt tcgcatttgc catattactg tctattatca
    10141 ttgacattgg tgcacaaatt aatatatggc gcatattagt tgtaactggt ttaagaggtc
    10201 aagaaatatc aaataaagtt gttcctgggc ttggtactgt tatctcaata ttgattgcat
    10261 ttggtggtct agcttttaat attggtaata ttgctggtgc cggtttaggt ttaaatgcaa
    10321 tttttggatt agatgtaaaa tggggcgcag ctattactgc aatctttgca atattaatct
    10381 ttgtaagtaa aagtggacaa aaaattatgg acgttgtttc aatgattctt ggtattgtga
    10441 tgattttagt tgtggcatat gtgatgtttg tttctaatcc accttatggt gatgcttttg
    10501 tgcatacatt tgcgccagaa catccaatga aattagtctt gccaatcatt acgttagttg
    10561 gtggaactgt aggtggttat attacctttg caggtgcaca tcgtatatta gactctggca
    10621 ttaaaggtaa gcaatattta ccatttgtaa atcaatcagc aattgcgggt attttaacta
    10681 caggtattat gagaacgtta ctattcctag cggtattagg agttgttgta acaggtgtga
    10741 cactaagttc tgaaaatcca ccagcgtcag tttttgaaca cgcaattgga ccaattggaa
    10801 agaatatttt tggtattgtg ttatttgctg cagcgatgtc atcagtaatt ggctcagcat
    10861 acacaagcgc aacattttta aaaacacttc ataaatcact taacgaaaga agtaatttaa
    10921 ttgtgattgt gtttatcgtt atttcaacga tgattttctt atttattgga aaaccaatca
    10981 gccttttaat tatagcaggc gcgataaatg gatggatttt acctattacg ttaggtgcaa
    11041 ttttgattgc aagtaaaaag aaatcaattg tcggtgatta taaacaccca aattggatgt
    11101 tcatctttgg tatcgttgca gtacttgtta caatattgac aggtatattc tcattcaaag
    11161 aagtacttca gttattttaa aagacatttc gactaatagc atcactgtaa atgttgttag
    11221 tattaggcaa gatgtatttt gatttccggt aattcattta taaagcataa atagatatat
    11281 ttgttgagag tggtgcatga caaatgtact gctctttttt gttgtgatga aaaatctttt
    11341 tctattgatt gagaaaatag ttatttatat agtttttaaa gaaaaaaagt gataatattt
    11401 agctaatcta ataaaaatgg atttattgag ctaaaaatac attttaaatg agtagaggag
    11461 gctacaaatg ggttacagaa ctttaaaaag tatttttcat gaacacaatg aaagtaaaat
    11521 gaaggaagag tattctaaaa gatttaattc tttagcttct ttcaatacta atattaatat
    11581 catacctatg gaaaatggaa aaaaagttaa tgatgtggaa tatcctctat tctttatggt
    11641 gactaaaaat ctatcaaaaa aacaagaatt aatatcaatt aatagtagaa aaattgatag
    11701 agcacttaat tctttacctt atgcagctag agaacaatat tttaatgatt tattaatcga
    11761 tgaattacaa agtactaatg aaattgaaaa tgtatttagt actaaacaag agattgcaca
    11821 tgcgttaaat aaccaagcat cagaatttct taagttcaga ggcctcgtgg atcaatataa
    11881 agaaatagaa cttaataaaa aaattaaagt tgataatgta agagacatta gagcgattta
    11941 tgataaatta gtttcaaatg aaattaacga acaagataag ttagatggag agctatttcg
    12001 taaaaatttt gtcggtgtgc atgatgggtc aacgaataaa tatatacatg ttgggttaca
    12061 acctgaaacc aaaattgttg aatatatagg tgaaatgcta acatttttaa aatattttga
    12121 tgcgcctcag ccgttcaaaa tcatggctag tcattatctg tttgaatata tacatccttt
    12181 ttatgatggc aatggtagag ttggaagatt tataattgcc aagctcttga gtgattatta
    12241 tgataattat actgctttga cattttcata tgtcataaat agaaataaat cgaaatatta
    12301 caaggcgttt atgactgctt caaatcattt gaattgtggt gatttgacgg aatttattga
    12361 cacaatgctt gagttattga tagcaggaca agaaagaata cttgatgagt taataccaaa
    12421 aatggatgcg actgaaaaat taacgcttta tttaactagt cattataaac agatagatta
    12481 tgaattttta tatctccttt caatggataa attgtttgga aataaaagaa atagattaac
    12541 attaattgat ttagaaaaca tattgggagt aggaagagta aaaattaata atacaattaa
    12601 aaaatttggt aactacttag ttaaaattaa aagtagacca acaatttatg agataagtga
    12661 tgaatttcta aattcga
//
LOCUS       DAPIDM010000066        12593 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000066 DAPIDM010000000
VERSION     DAPIDM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.066"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(86..1267)
                     /gene="sspB"
                     /locus_tag="RJE42_001619"
     CDS             complement(86..1267)
                     /gene="sspB"
                     /locus_tag="RJE42_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDV5877036.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLANNIKEKESAKTVSPQLKQELKTTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYNQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(1349..2377)
                     /gene="sspA"
                     /locus_tag="RJE42_001620"
     CDS             complement(1349..2377)
                     /gene="sspA"
                     /locus_tag="RJE42_001620"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDV5877037.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQT
                     DKQQTPKIQKGGNLKPLEQRERANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNQFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPNNPDNPDNPNNPDNPNNPDNPNNPDQPNNPNNPDNGDNN
                     NSDNPDAA"
     gene            complement(2905..4059)
                     /locus_tag="RJE42_001621"
     CDS             complement(2905..4059)
                     /locus_tag="RJE42_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV5877038.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYINAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(4255..5265)
                     /locus_tag="RJE42_001622"
     CDS             complement(4255..5265)
                     /locus_tag="RJE42_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV5877039.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            5418..5837
                     /locus_tag="RJE42_001623"
     CDS             5418..5837
                     /locus_tag="RJE42_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5877040.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            5864..5974
                     /locus_tag="RJE42_001624"
     CDS             5864..5974
                     /locus_tag="RJE42_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877041.1"
                     /translation="MSLCTQQKLNHALQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(6045..9791)
                     /locus_tag="RJE42_001625"
     CDS             complement(6045..9791)
                     /locus_tag="RJE42_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDV5877042.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKAANKTGNAQVNQKVDTTQVNG
                     DTRATQSTTSNNAKPVTKTTNTTAPKTNNNVTSAGYSLVDDEDDNSENQINPELIKSA
                     AKPAALETQYKAAAPKATPVAPKAKTEVTPKVTTFSASAQPRSAAAAPKTSLPKYKPQ
                     VNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEI
                     SYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFAR
                     SMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNY
                     SYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTGKLTVAANNGVAQIKPTNS
                     GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNT
                     AKSPVNVNQTYSIKPGTKLYTVPWGTSKQIAGSVSGSGNQTFKASKQQQIDKSIYLYG
                     SVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTT
                     VYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPV
                     NVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLYGTVNGK
                     SGWISKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPNNTGIRASVY
                     EKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVK
                     TTQKYTVNKSNNGLSMVPWGTKNQVILTGDHIAQGTFNAAKQVSVGKDVYLYGTINNR
                     TGWVNAKDLTAPTAVKPNTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQP
                     FSVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQY
                     KPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKG
                     KGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTK
                     YHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRW
                     NPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(10019..10453)
                     /locus_tag="RJE42_001626"
     CDS             complement(10019..10453)
                     /locus_tag="RJE42_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877043.1"
                     /translation="MFTKVIDPEMLEDCFYIRKKVFVEEQGVPEESEIDQYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVIKSHRGQGMGKMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(10609..11079)
                     /locus_tag="RJE42_001627"
     CDS             complement(10609..11079)
                     /locus_tag="RJE42_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDV5877044.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(11127..12344)
                     /locus_tag="RJE42_001628"
     CDS             complement(11127..12344)
                     /locus_tag="RJE42_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDV5877045.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVDSIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNTDDSNNNNSVNNQQQPATDQNLNQNQSGTQQ
                     TPQASNNQNGVVN"
BASE COUNT         3902 a         1928 c         2050 g         4713 t
ORIGIN      
        1 gagtttggtt gtgtcgtaaa ctaaatttat aaattgtagt tgatacatag ttttacctgc
       61 ctttaatcaa tatctatatt actttttagt aacctatcat tgaaccatac cagttataat
      121 cacgattgaa tgataaatgt aataggcttg aatctgcatc ttggattgat aattctgtat
      181 cccaaggatt ccagtaaata agtttttctt ggtcattaat tttagcatta ccaacaactg
      241 ctagggcatg acctaaatgt gggtcattag gattttgaga tacactttga gcaagaatca
      301 taatacccac attatctttt gtaagttgat caacttgatt atatgatggt acgccttctt
      361 gataatgaat gtctctgcct tgtgatttac cgtattcaat catttgatta gggaatgttg
      421 cacaattagg aaggtcttgc tcacttactt cagggtataa tgtacgcata atatcatgtg
      481 cattataagt gtctgtattt ttagttgcat ttaataatgc tgccatactg aatcctgcac
      541 accatgagtt atcgaattgt tgttctctaa ttttgaagtt ttttagtgtg ttttcatatt
      601 gaacttgatc ttcttgtatc gcttcgtttt cttcaacttt tgtaggtgtt actgtagttt
      661 ttagctcttg ctttaattgt ggtgaaactg ttttagcact ttctttttct tttatgttat
      721 tagctagtgg cgtagctttt actaatctaa ctttgccatc ttcttcaaaa taaaatcctt
      781 tttcatcagt aagaacagtg atatttgaat ttttatcttt aatttggtct aaatctttag
      841 cgatgaagtt tgaaatttta acactgtaat tcatgtcttc ttttgatttg tttaaatcat
      901 ctttattttt agggctaagt gttaaagtat aaacaatttt accgtctttt ataactggat
      961 aatagtaact attatcttct tcgccattaa atttataaat tttaaaagct tcgccaagtt
     1021 catatttacc agtttttgca ttactttgtt tatctaacgc tttagcataa ccagcaaatt
     1081 gttgttgagc tagatctttt actttttgag gtactttgtc actcttaaca ttgatttcaa
     1141 gttgttttga atgtgaatcg gctttggctt tattgttatt agcaaatgcg cctagtgata
     1201 aaataagcat tgaaaccatt atgatgctta taatattgaa tactctagat ttacatgaac
     1261 tattcatata ataccctcca tttaagtcaa aatattttgc gttgctaagt ctttgcttct
     1321 attttatcta gacgaactta attgttaatt aagctgcatc tggattgtca gaattattgt
     1381 tatcgccatt atccgggtta tttgggttgt taggttggtc agggttatta gggttgtccg
     1441 ggttgttagg gttatcagga ttattaggat tgtctggatt atcagggttg ttagggttat
     1501 caggattatt aggttggtca tcgttggcaa aatggatatc ttcaatattt tgttttaaga
     1561 agttgcgtac attttcatta ataaatactg caccgttaaa ttgatttgga acgccacccc
     1621 aatgaatgcc aatgacttca tttttttcat taaatacagg tgaacctgag ttaccaccag
     1681 ttgtacttaa atcatattgc atagcttcac cttttaagta cgttattttt cctttacttt
     1741 cccacattgt tgcgacaggt ttatcaccag gatatcccgt tacagtaata ttttggttaa
     1801 cttgtgtttc agcattatta ctcattgttg ctggtttaac tacttcacca atatgtttgt
     1861 tttgctcatt aggggagaat ttaactattg ctaaatcacc ttcgcctgaa tatttagtga
     1921 tttgttcagc agtgaaacca ccatttggat aattatcttg gttaattgca gaagggaatg
     1981 cttttaaagc atgaggatca ccgtgcgtag catctacgac gtgtttattt gttaaaagag
     2041 tatctttacc tacaactaca ccagaagcaa taaatgtacc agtaggtgct tcaacttgaa
     2101 tataagtaac aggtgcataa tgaccattcg ttgtatctgt gatttggtga cgatcgttat
     2161 ttggtaatat aacattagcg cgttcacgtt gttctaatgg tttaaggtta ccgccttttt
     2221 gaatcttagg tgtttgctgt ttgtctgtct gcgtttgttg tggatgattg tccatagctt
     2281 ttgaagataa cgcatttgct gctggagaac tcacaagtgt cgctgttgtc aaagttgcaa
     2341 cgaataaaga actaactttt aaaaatttac ctttcatcta aaaacctcca aaaaaattat
     2401 ttacaagtta aatataacac taaaaatttt taagtcaata agaatatata aataaattaa
     2461 aaagtttttg taaagattag agtttacaaa aacattaaga ggattaaaat tgaattgtat
     2521 tacactttat tagattgtgt taaattggta aggtcgtcaa ttgtttgcat ttatctaaaa
     2581 gaataaataa gaaaggaatt gagagcgaag agaaagtatt tgtttataat aataaatagt
     2641 aacgtttgaa taataattgt ttggtcaaag gtagattaat atgaatgctg atatattatt
     2701 tgagtgctta agtttcgaag aacaattatc agttagcata ttaaaagctt ttgatagaat
     2761 cattttgagt gaggtgttta aaggagagga atataactcg gtagtattta cagtctcttt
     2821 agagtattat ttaaaacata tagaatataa aaaactgaac gtactatgaa tttcgttcac
     2881 gacgtccagt tcaacatatc atttttattt agtatttaaa tatttagcaa gtctcttcat
     2941 accttctttt aaaacatcca tttcataagc ataggaaatc ctcacaaatc ctttaccgaa
     3001 ttctgtaaag gatgagcctg gaacgattgc taaatgtgtt gattcaagta aatcgacaca
     3061 aaattcgaaa tcgtcatcgg tgatatgttt aatacttggg aaaatataaa acgcgccttc
     3121 aggttgagcg gtaatctcaa accctaattt agttaattca gatactaaat aatttcttcg
     3181 ttctacataa gcttcattca tatatttagg agcttctaat ccttcgttaa gtgctgttat
     3241 acatgctatt tgagctggaa cattggcaca aatacaatta taggcgtgca taaatgttaa
     3301 tttatcaatc aaatattgag gtcctaatag aaaaccaatt cttattcctg ttgctgagtg
     3361 tgatttactt aaaccaccaa ttaatattaa ttgatcacga atgtcttcaa attcagcgaa
     3421 ggatacatgt ttaccactaa atgtattttc agcataaatc tcatcgctaa taataaatat
     3481 cggatatttt tttaatacat ttacgatatt taaaacttca tttcttttta aaactacgcc
     3541 agttggatta gtcggataat ttaacaagac agcttttgtc tttggagaaa tatgactttc
     3601 taacgcatca ggtgtaattt tgaattgtgt tgctgttgta tcaatataaa ttggtttacc
     3661 acctagtact tcgataagtg gtatgtagcc tgcataaatt ggtcccggta ttataatttc
     3721 atctccaggc tctatgatag aacgtaacgt tgtgtctatt gcttcacttg ctccatttgt
     3781 cacaataatt tcttcaggat cataggaaaa atgataacga tttttgaagt attgactaat
     3841 tgcttcgcga gtttctaata agcctttatt gtgagagtat gatgtcttgt cattgttaat
     3901 agcatttata tatgcctttt tcacaacatc aggcataggg aagtctggtt ggccaatagt
     3961 taaattaaca caatcatcca aattattcat acgatttgaa aattggcgaa tacttggtgc
     4021 tcttaaatat ttagaattag aatttaaaga aagtttcatt tgtgacacct caaatataaa
     4081 tcaaatattg tctaaaaatt tagaaattaa taatatcata acatactttt aaagcaaaaa
     4141 agggtaaatt acttaagctt tatttgaaaa atgaaaaatg tctaaataca ctgtagtaac
     4201 tacttttcaa aatgaatgaa gtggttacag ttaatgtact tagacattat ataattatga
     4261 gtctttaaac tgtgatggtc tttgtaaatt aattattgga tttgtccatt tacaaacaaa
     4321 gtttgtagat aatacatata cgatgattac agatatactt attaaataaa gatacgtcat
     4381 taatgatata ggattatcga atgggtacca ttcaaatcct ctaacaatgc caataattaa
     4441 accatgtaat aaatatacgt atagtgtacg actaccaata taagtatata attttttctt
     4501 tgttgacatt aaatttagaa acgcagtcat tgcgattaat ataattccat ataatataag
     4561 tcgtttaaaa ggactgaata cactctgtcc ttcattttca agtgaagtat atggtgaact
     4621 tcctaataac caatctgcat tgataggatg aatcacgtaa atgataaaaa ataaaataaa
     4681 cgtaatgata gatactggta ttagtttttt atttttaaaa atagccgtat gttttttggt
     4741 gaaaatgtag cctaggtaaa atattgggaa aaatacgatt gtccttgaaa tgcttaagta
     4801 gctatcgatg ttatctgaaa aacctgctcc aatagatata ataattgaaa ctgatagtac
     4861 tttatatgga ttaaatcttc taactattac taaaatgaca tgaaagaaaa atagcgtgat
     4921 caaaaaccat aacgcaaata ctgggttaaa aggatcaagt tgtaattcgt cacttttacc
     4981 tgttaagaaa taatatattg aaaagaatgc aaaaaatatc atataaggta ctatcaaacg
     5041 ttttgaaatt ttttctaaat agtatggttt atcaatattt ttcgcgaaat aaccagatat
     5101 aaacaaaaat gttggcatat ggaaactata aataactagg tataatgctg ataaatattt
     5161 atcgccacta gtgtagggtt gtaacatatg tccaaatacg actaataata ttaaaattgc
     5221 tcttgcgttg tcaaaaaaat aatctctctc ttttaatgaa gtcatatttt atctccttta
     5281 tgtccataag actcttacta atttattata ttttgtatta gttatgcaac aaatttgtct
     5341 attgtgtcga atagtattgt aaaaattata aaaaaagcat ataatattat aaataatatg
     5401 tagtggagta gaacaacatg tataaacaac ttgaaaaact tattacactg actaacaatg
     5461 acttaaactt agtgaataga agatttggac aacgcacgga tatcacatct gaacagctag
     5521 aacttctccg tattttattt aattacgatc gcttatcaca gtatgatttg acgatgaaga
     5581 ttagcaggga acaatctata gtttcaaggt ggattaagaa attagttttg aaagggtaca
     5641 tcacaagtca acaatctagc gaagatttaa gatgtaaaga attaattttg actgatcaag
     5701 cacgtacatt gatttcacaa ataaataatg cacgttgcga attgattgaa gcaagatgtc
     5761 aatgtttatc ggaagtcgaa ttagacaatt taaatcaatt acttgataag ttaaatcaac
     5821 gacgcatatc gttgtaacac ggcaatagca aacacataat ttagtgtcat tgtgcacaca
     5881 acaaaaattg aatcatgcat tacaagcaaa agtagcggtg attgttaaaa ttgatggtaa
     5941 acaatcaccg ctatttttgc ttgtgtatgt ataaaaaaag ggatcaaagg tcatccccca
     6001 tgattgatag tgggggatga cttttgatcc tatgttcatg ttgcttattt atattgtggg
     6061 atgtcgaagt atttgccgac ttcgccaatt ttatcatagt agcctttgat gattttagca
     6121 ttgatgttag cccaatctac atctgtagca tattggtgtg ttcctggatg tgcaggattc
     6181 catctcattt tgtaaagcgt attttgacca gctttaacat atgagttgcc gatgaattta
     6241 gcaccaccaa cgattgcttt tgatactgtg tcccaaccag cttgtttagc atatttaata
     6301 ccttcacgta aaggatcgtt atcatatgca gcaataccaa atacgttatg gtatttcgtg
     6361 tttgagttag ttacaacttt gttgttcact acatctgcac cttttgctaa ttgagaagta
     6421 ccgttacctg tttctaatag ggcatgtgag ataagataaa cttcattaat gccatacatt
     6481 tgagcagctt tgttaaatgc agcaccttgg ttttctaata cacctttacc ttttaagaat
     6541 tgattaattt tatcaataga aatattttgt ggttggtcta agcgtaagaa ttgatatttt
     6601 aatgctggat cttgagctaa acgcttcgta tccattgcat gcttaacatc attaaagtta
     6661 gcatctgtcc acttacctgg tacacgttgt acttgtggtt tatattgtaa accagcttgt
     6721 atttgagcaa cttggtttaa tgtcatacct gtttgattat acttaattaa ttctttagct
     6781 aaatcagttg atttaatcca tgctaattta ccgttagata atttaccata gtaccaagtt
     6841 tgtccattaa tgacttgttc tttaacaact gagaatggtt gttcattaaa tgcttttaat
     6901 gagtatttag ctgtatcaga atttggtgtt acatagtaat aaccattacc gtttttaatt
     6961 acataagtgt agttgtaatc ttttgcagct gatgtatttg gtttaacagc agttggtgca
     7021 gttaaatctt ttgcatttac ccaaccagtg cggttattaa tagttccgta taaataaaca
     7081 tctttgccta cagatacttg tttcgctgca ttaaatgtac cttgtgcaat atggtcacct
     7141 gttaaaatga cttgattttt agtaccccaa ggaaccattg ataagccatt atttgattta
     7201 ttaacagtat atttttgtgt tgttttaact tctttgccta agttttgaac atttaagtct
     7261 ttaacattga accaacctaa tggaatatta tgacttgtat tatttaataa tacgtatgtt
     7321 tcattaccat gtgcacgttc ttttgttaca tagaacgtac ggtctgcata ttttgcgccg
     7381 tttttcgctg ttttttcata aacagaagca cgaataccag tgttgtttgg tttaacttgc
     7441 gcaattttgc taactgtttg agtcgtttga ggtttagtaa cagcataagc ttttacagct
     7501 gtttttggtt gtgctactgc ttttttaggt gcagcaggta cagctaaata tgctttactt
     7561 atccaaccag atttaccatt tacagttcca tataaataga tagatttatc aatttgttgt
     7621 tgcttagtcg ctttaaaagt ttggttacct gtaccagata ccgcaccagc ttcttgttta
     7681 taagtacccc aaggtactga atataattta gtgcctggtt ttactgtata tgtttgcatt
     7741 acatttacag gtgattttgc attgttataa ataacgtcac cttgtttaac ccaaccaatt
     7801 aaagttggac tattgtaatc tttaactaag tagaatttgt ttccacctag acttgcctct
     7861 tttgttacag caaatgtttt ttgaacttct ttcgttggct taccagtttt gtcataaact
     7921 gtagtgaata agccattgtt tttagcatta atttgagcaa caccgtttaa tgatgaaact
     7981 gttaatttat tatttgttgt aggtgttgat ggcttaggtg ttggtgtagg cgtaggttta
     8041 gcagtatcaa ctaaatatgc tttacttacc caaccagatt taccattcac agagccatat
     8101 aaataaattg atttatcaat ttgttgttgc tttgaagcct taaatgtttg atttccagag
     8161 ccagacacgc taccagcaat ttgtttagat gtaccccaag gtactgtata aagtttcgta
     8221 ccaggtttga ttgaatatgt ttgatttaca tttacaggtg atttagctgt gttgtaaacc
     8281 acatcgcctt ctttaaccca accaaattta ttaccagaat tgtaatcttg tacaagatag
     8341 aatttttgat tacctaatgt agctgtttta gatacagcaa atgttttttg aacttcatta
     8401 gttgctttac cagttttgtc ataaacagta gtatataaac cactattagt aggtttgatt
     8461 tgtgcgacac cattattagc agcaactgtt aatttaccag ttgatggttt tgatggtgta
     8521 gtaggggtag ttgtagattg cgtaccccat ggcgccactt tacccatttt tattaaatat
     8581 ttttcattaa ttaagtcata taattgatca taactataat tatgacttct taaatatcca
     8641 tgtggatcgg catggtccgt accacctaaa tatttactta cagcatagtg agtccatact
     8701 gtaccatttc catcatactc agcactgtct ggttttaaac cataatattg taattgtgta
     8761 gctgcatagt cagcatagtt attcattgaa cgtgcaaatg atgcatagtc gtgtgtgtgc
     8821 acgatttcaa cattgatgaa tctagggtta ccgactgcac cgacacccca agataagtaa
     8881 tccgttggtg ctgtttcgat tatacgatcc ccatcaacaa atgcatgaac gaaagcattt
     8941 tgataattgt ttttcatata actaatttcg ccatttatcg tcgaacgatc attagctgta
     9001 tcatgaacta cgattccttc aggacgacct acaccgttac ggtatgcgta tttagggaag
     9061 taagatgtat aatcttcttc aatcttaggt gcttttaaat tatttttacg aatgtaatca
     9121 ttaattgatg agtttacttg cggtttatat tttggcaaac tcgtttttgg tgctgcagcg
     9181 gctgatcttg gttgtgctga agcactaaac gtagttactt ttggtgtaac ttcagtttta
     9241 gctttaggtg caacaggtgt tgcttttggt gctgcggctt tatattgcgt ttcaagagca
     9301 gcaggtttag cagctgattt aattaattct ggattaattt gattttctga attatcatct
     9361 tcatcatcaa ctaaactata tccagcactt gtaacattat tgttcgtttt aggtgctgtt
     9421 gtgttagttg tttttgtaac aggtttcgca ttatttgatg tagttgattg agtcgcacga
     9481 gtgtcaccat ttacttgtgt agtatcaacc ttttgattta cttgagcatt gcctgtttta
     9541 ttcgctgctt ttggttggac aatagcaggg tcttgatata cttgagtgcc agaaatgttt
     9601 tgcgttggat tttttacctc agcttttgct tgttcagtag ttgctttaac tttattacta
     9661 tctaaaacat ttttattagt agtttgatct tgtgtcgtct cagctgcttg aacttgatgt
     9721 gcagtgactg ctgaacctac tagcgttaat gcaaccattg atggtagttt gtaattaaat
     9781 tttttcgcca ttctatttat tactcctaac atttattaat tattaatctc attatagtac
     9841 ctgttttata cacctgtgcg tatttaacca atttgtaata tactcttaat ttatattttc
     9901 actgtaaaaa aatgaaaaaa attttgaatc tcaatggtga cgtgacaaat caaatagaat
     9961 tgatgtcaat ttaagaattg gtttattaca aatgtttcta taatagatac aacttttttt
    10021 aattaagcga ggtcaacttt tttgtcattt caatatgctc gatgccttcc tcaagaaata
    10081 tattacctct cattttaaaa tttaaacttt catagaatgg gattgcatga cattgggcat
    10141 tcatggtagc tacgtaaaaa ccttcatctt tagctaatga ttctacagct tgcataagca
    10201 tttttcccat gccttgtccg cgatgtgatt ttatcacagc cactcgttct attttgacag
    10261 ttgtttcatt gataggacgt atacgagctg tggcaactgg ctgtccatta tcatatccaa
    10321 tgaggtgaat agattcagat tcatattgat caatttcact ttcctctggg acgccttgtt
    10381 cttctacaaa cactttcttt cttatataaa agcaatcttc taacatctct ggatcaatca
    10441 cttttgtaaa cataaaattc ctccatatcc taataaaatc atttacattt aatttactaa
    10501 taataacata gaataacgat tgttattttc cacaaaaata attagacata aaaaagttat
    10561 aaagatgatt cacaggaatc atctttatag ctagatagag tgtatttgtt ataatgcttc
    10621 ttttctaata attgctgtat attgccaaaa gattcgcatt tgagcaatat tccaattatt
    10681 cattcccatt gtaaacccag aaggtttaaa tatattcaca tctgtcactt ctaaagctgc
    10741 tgcgactaaa tattgtaatg gaatactaag tgatttcatt gtttcggtaa taccattttc
    10801 atcatatacc catgaaaatg gtaactctga ttcgaaaaca tctacactat tgaaaatttc
    10861 aggtagggct aaaatataac atgcaccatc tacaatagga ttctcgatac tatttttgta
    10921 ataaattagg agtgcttcat agttttctcg atgctcatga ttgacgtaaa aaaattcact
    10981 tctaaaatga gccatatctt ggctatgaat aatttgttgt tctaacatat taaacatccc
    11041 atttatattt gcaatctttt cgtatgtttt tctactcatg tatgatactc ctttcataaa
    11101 tagtaaatgt taatcgtcat ataatattag tttacaacac cattttggtt atttgaagct
    11161 tgtggcgttt gttgagtgcc actttgattt tgatttaagt tttgatctgt agcaggttgt
    11221 tgttgattat tgacgctatt gttgttatta ctgtcatcag tattattcga agatttatct
    11281 ttatcagtgg tatcgttttg ttcttttctt aataaactac tatctaaagg tgttaaaggt
    11341 atgagagaac cataatgatt aatgacacgt tgatctaaga aatcattttt atcattaata
    11401 ggtgataatt ctaagtcttt acgaagtaag tttgcatatt tttgaatgct gtctacactt
    11461 ggatgataat agtaaatacc atttaacata tcatctttac cttttaattg cgcagtttta
    11521 atttcaacat catttgttaa gtacattttt gctaaagctt taatttcaga gttagttaaa
    11581 ttatgctttg catttttacc tacaatttga atgacgttat caagtttatc aatagaatca
    11641 acttcctgtg ctttttggaa taaaatctta attaattcca tttgacgttg tccacgtttc
    11701 aagtctgaat catgatgtct agttctagca actgctaaag cctcatcacc atttaatttt
    11761 tggtaccctt ttttaatttt aatcttacca gtatcatctg tgtttggctc atttaaatca
    11821 tatggtacat catagtatat gccaccaagt tcatttacag cttctacaaa ggccttcata
    11881 ttaacgcgta catagtaatc aactggaaca ttcatcgttg cttcaacaga gtccattgct
    11941 gcaattggac caccatatgc atgtgcatgg gtaatcttat cgtaatagcc gactttagga
    12001 atgtagctga ttgtatcacg aggaatacta agcattcgaa tttggtgttt agattgatta
    12061 aaagtagtta aaatcatagc gtctgatcta gagtgttcag catcttggcc tttttttctt
    12121 cttccgtcat tatcatcgat acctaagaag agaatagaga taggttgttc ttcaggattg
    12181 actttattgt ctcgtaaatt tgattgacga ttagcatttt tactatcttg agaagattca
    12241 aatgcatctt gagacgtttt aaaaagtaac gtagcgaaga caattggaac aacaataaga
    12301 accaatgcta gaaggatcaa aaagtatttt aaaaatttat tcatgattga tgctcctata
    12361 ttaaattttt gttaaatcat aatcataatt ttaaaatata attagttgaa cgatatttat
    12421 acaacttcat tactaataca ttatagcctt aaattgtagt actttatagt aaaaaataca
    12481 aatgttatgt caaagttcct tgtaatttga atgttttgtt aaaatcaaaa agcattttaa
    12541 aaaatataat gcgactttag tatgatgtct taaaaaaatt aaaggggtaa tat
//
LOCUS       DAPIDM010000067        12543 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000067 DAPIDM010000000
VERSION     DAPIDM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.067"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            160..1101
                     /gene="pde2"
                     /locus_tag="RJE42_001629"
     CDS             160..1101
                     /gene="pde2"
                     /locus_tag="RJE42_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDV5877046.1"
                     /translation="MISTMNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLKQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLAKALRTKLN"
     gene            1122..4319
                     /locus_tag="RJE42_001630"
     CDS             1122..4319
                     /locus_tag="RJE42_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV5877047.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDTCIANNIKPIFGMTIYVTNGLNTVETVVLAKDNNGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKNVADEHRDIVQVFDSHEDTYLDHRSVLVQGIKHVWIQD
                     VCYQTRHDADTISALAAIRDNTKLDLIHDQEDFGAHFLTENEIQQLDVNPEYFTQADR
                     IAQKCDAELKYHQSLLPQYQAPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRREKVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVEERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            4768..5997
                     /locus_tag="RJE42_001631"
     CDS             4768..5997
                     /locus_tag="RJE42_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDV5877048.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            6192..7049
                     /locus_tag="RJE42_001632"
     CDS             6192..7049
                     /locus_tag="RJE42_001632"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDV5877049.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            7049..7993
                     /locus_tag="RJE42_001633"
     CDS             7049..7993
                     /locus_tag="RJE42_001633"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDV5877050.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            8262..9230
                     /gene="pfkA"
                     /locus_tag="RJE42_001634"
     CDS             8262..9230
                     /gene="pfkA"
                     /locus_tag="RJE42_001634"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV5877051.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGTVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            9252..11009
                     /gene="pyk"
                     /locus_tag="RJE42_001635"
     CDS             9252..11009
                     /gene="pyk"
                     /locus_tag="RJE42_001635"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV5877052.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIEEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(11353..>12543)
                     /locus_tag="RJE42_001636"
     CDS             complement(11353..>12543)
                     /locus_tag="RJE42_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5877053.1"
                     /translation="RGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITG
                     MAEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATII
                     GLIVVGFVMILFAYKTQFGHASFTNLYEHGVFPKGASGFFMSFQMALFSFVGIEMIGV
                     TAGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIG
                     IPFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIF
                     ASSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKK
                     ATYKLFGGKYMGYLIFVFFIFVFGLLFINVETRRAIYFIPIWFILLGFMYLRYKRIAA
                     KSNN"
BASE COUNT         4481 a         1740 c         2406 g         3916 t
ORIGIN      
        1 tgttggggcc cctgaactaa atagtatgaa aaaaatatat atctaaataa ttaaatcggg
       61 caaatagttt aatatggtag aacaattttt ataatttaga tattttgata tagactaatg
      121 cttaaattaa gcaatttttc ttgaaaaggg agaatgaaaa tgattagtac gatgaatgaa
      181 attatgaaat gtattgaaga caacgataca attattatac acagacatgt tagaccagat
      241 cctgatgcat atggttcaca acttggttta aaatactata ttcagcagaa atttccgcaa
      301 aagcaagttt ttgcagtggg tgaagcggaa tcatcattaa gttttattgg agagttagat
      361 aacattgatg ataaaacata tcaagatgcg cttgtaattg tatgtgatac tgccaatgct
      421 ccacgaattg acgatgaacg ttatagtaca ggtcgtaaac ttattaaaat agatcatcat
      481 cctgcagttg atcagtatgg tgatattaat ctagttaata cgaacgcgtc atctacaagt
      541 gaaatcattt atgatttaat ctcacatttt aatgatgaag caattgttaa taaagatatc
      601 gcgagtgttt tataccttgg tatcgtcggt gatactgggc gattcctttt taacaatacc
      661 tcagaacata ctatggaaat tgctggaaag ttaattgggc atgatattga tcataatgcg
      721 cttttaaata aaatgatgga aaaggatcca aaaatgttgc cgtttcaagg ttatgtttta
      781 caacatttcg aacttatgga tgacggattc tgtcaagtta aaataactga agatgtattg
      841 aagcaattcg gtattcagcc aaatgaagca tctcagtttg ttaatacaat tgctgacatc
      901 aaaggtttga aaatatgggt gtttgcagtt gatgagggta gtgaaataag atgtcgctta
      961 cgctctaaag ggcaattgat tattaatgat attgcgcaag attttggtgg cggtggtcat
     1021 ccgaatgcgt caggagtttc agtgaacagc tgggatgaat ttgagcaact tgctaaagct
     1081 ttgcgcacga aacttaacta atagaaaggg gccattcaat catggtggca tatttaaata
     1141 ttcatacggc ttatgatttg ttaaattcaa gcttaaaaat agaagatgcc gtaagacttg
     1201 ctgtgtctga aaatgttgat gcacttgcca taactgacac caatgtattg tatggttttc
     1261 ctaaatttta tgatacatgt attgccaata atataaagcc gatttttggt atgacaatat
     1321 acgtgacaaa tggattaaat acagtcgaaa cagttgttct agcaaaagat aataatggtc
     1381 tgaaggattt atatcaacta tcatcggaaa taaaaatgaa tgcattagaa catgtgtcgt
     1441 ttgaattatt aaagcgattt tctaacaata tgattatcat ttttaaaaat gttgcagacg
     1501 aacatcgtga cattgttcaa gtttttgatt cgcatgaaga tacgtattta gatcatcgaa
     1561 gtgttttagt tcagggtata aagcacgtat ggattcaaga tgtatgttat caaacacgac
     1621 atgatgcaga tacaatttct gcactcgcag cgataagaga taatacaaaa ttagacttaa
     1681 ttcatgatca agaagatttt ggtgcacatt ttttaactga aaatgaaatt caacaacttg
     1741 atgtgaatcc agaatatttc acacaagctg acaggatagc tcaaaagtgt gatgcagaat
     1801 taaaatatca tcaatctcta cttcctcaat atcaggcacc taatgatgaa tcagctaaaa
     1861 aatatttgtg gcgtgtctta gttacacaat tgaaaaaatt agaactgaat tatgacgtct
     1921 atttagagcg attgaaatat gagtataaag ttattactaa tatgggtttt gaagattatt
     1981 tcttaatagt aagtgattta atccattatg cgaaaaccaa tgatgtgatg gtaggaccag
     2041 gtcgtggttc atctgcaggg tctctggtca gttatttatt gggaattaca acgattgatc
     2101 cgattaaatt caatctatta tttgaacgtt ttttaaaccc agaacgtgta acaatgcctg
     2161 atatagatat tgactttgaa gatacacgcc gagaaaaggt cattcagtac gtccaagaaa
     2221 aatatggcga gttacatgta tctggaattg tgactttcgg tcatctactt gcaagagcgg
     2281 ttgctagaga cgttggacga attatggggt ttgatgaagt tacattaaat gaaatttcaa
     2341 gtttaatccc acataaatta ggaattacac ttgatgaagc atatcaaatt gacgatttta
     2401 aaaaatttgt acatcgaaac catcgacatg aacgctggtt cagtatttgt aaaaagttag
     2461 aaggtttacc aagacataca tctacacatg cggcaggaat tattattaat gaccatccat
     2521 tatatgaata tgccccttta acgaaagggg atacaggatt attaacgcaa tggacaatga
     2581 ctgaagccga acgaattggg ttattaaaaa tagattttct agggttacga aatttatcaa
     2641 ttatccatca aattttgact caagtcaaaa aagatttagg tattaatatt gatattgaaa
     2701 aaattccatt tgatgatcaa aaagtgtttg aattgttgtc gcaaggagat acgactggca
     2761 tattccaatt agagtctgac ggtgtaagaa gtgtattaaa aaaattaaag ccggaacact
     2821 ttgaggatat tgttgctgta acttctttgt atagaccagg tccaatggaa gaaattccaa
     2881 cttacattac aagaagacat gatccaagca aagttcaata tttacatccc catttagaac
     2941 ctatattaaa aaatacttac ggtgttatta tttatcaaga acaaattatg caaatagcga
     3001 gcacttttgc aaacttcagt tatggtgaag cggatatttt aagaagagca atgagtaaaa
     3061 agaacagagc tgttcttgaa agtgagcgtc aacattttat agaaggtgca aagcagaatg
     3121 gctatcacga agatattagt aagcaaatat ttgatttgat tctgaaattt gctgactatg
     3181 gttttcctag agcacatgct gtcagctatt ctaaaattgc atacattatg agctttttaa
     3241 aagtccatta tccaaattat ttttacgcaa atattttaag taatgttatt ggaagtgaga
     3301 agaaaactgc tcaaatgata gaagaagcaa aaaaacaagg tatcactata ttgccaccga
     3361 acattaacga aagtcattgg ttttataaac cttcccaaga aggcatttat ttatcaattg
     3421 gtacaattaa aggtgtaggt tatcaaagtg tgaaagtgat tgttgaagaa cgttatcaga
     3481 acggcaaatt taaagatttc tttgattttg ctagacgtat accgaagaga gtcaaaacga
     3541 gaaagttact tgaagcactg attttagtgg gcgcgtttga tgcttttggt aaaacacgtt
     3601 caacgttgtt gcaagctatt gatcaagtgt tggatggtga tttaaacatt gaacaagatg
     3661 gttttttatt tgatatttta acgccaaaac agatgtatga agataaagaa gaattgcctg
     3721 atgcacttat tagtcagtat gaaaaagaat atttaggatt ttatgtttcg caacacccag
     3781 tagataagaa gtttgttgcc aaacaatatt taacgatatt taaattgagt aacgcgcaga
     3841 ataataagcc tatattagta cagtttgata aagttaaaca aatccgaact aaaaatggtc
     3901 aaaatatggc attcgtcaca ttaaatgatg gtatcgaaac tttagatggt gtgattttcc
     3961 ctaatcagtt taaaaagtac gaagagttgt tatcacataa tgacttattt atagttagcg
     4021 ggaaatttga ccttagaaag caacaacgtc aattaattat aaatgagatt cagacattag
     4081 ccacttttga agaacaaaaa ttagcatttg ccaaacaaat tataattaga aataaatcac
     4141 aaatagatat gtttgaagag atgattaaag ctacgaaaga gaatgctaat gatgttgtgt
     4201 tatcctttta tgatgaaacg attaaacaaa tgactacttt aggctatatt aatcaaaaag
     4261 atagtatgtt taataatttt atacaatcct ttaaccctag tgatattagg cttatataac
     4321 ttttatcaac taataaatta tgatatagta aactgatggt tagatatttt ttaaccatca
     4381 gtttacgttt atattagttt tttatgctta ttatttttat gagtttcact ttacattatt
     4441 gattaatcca taagaataat tagcacaaaa agcagtatac ataaattgag taaagaattt
     4501 tgtcgatata tcttgagaaa aaataataac tttttgatat attggttttg ctatccatta
     4561 taataattaa aggtatatct ttagatagta tggtttaatg tatctttata aatattattt
     4621 tattatcgtt tatcgctatt tgtgttacat aattaaaact ataagttctt actcattcag
     4681 atttacccaa tatttttact tttataatgt aatgcgtttt atccaagtta ttttttaaaa
     4741 ataaatattg aattaggggc tgatttcatg tcattaagag atgaagcatt ggaaatgcac
     4801 aaacgtaatc aaggtaaatt agaagttaaa ccaaatgtaa aagttactaa taaagaagaa
     4861 ttaagtttag catactcacc tggcgttgct gaaccgtgta aagatatata tgaagacaaa
     4921 agaaaagtat atgattacac aattaaagga aatacagttg cagttattac tgatggaaca
     4981 gcggtattag gtttaggtaa cattggacct gaagcaagta ttcctgtaat ggaaggtaaa
     5041 gcagtattat tcaaaagctt cgctggtatc aatggggtgc ctattgcgtt aaatacaact
     5101 gataccgaag aaatcattaa aacagttaaa ttgttagaac ctaattatgg tggtattaat
     5161 ttagaggata tttcggcacc acgttgtttt gaaattgaag aacgattgaa aaaagaaact
     5221 aatattccgg tattccatga cgatcaacat ggtacagcaa ttgtaacaat ggcaggtttg
     5281 gtaaatgcat tgagagttgt taacaaagat attgctaaaa taaaagttgt actaaatggt
     5341 gctggtgcag caggaatagc cattgttaaa ttactatacg cgtatggtgt aagaaatatg
     5401 gttatgtgtg actcaagagg cgcaattttt gaaggacgtt catatggtat gaatcctacg
     5461 aaagatgttg tagctaagtg gacaaataaa gacaagattg aagggtctct agaagaagtc
     5521 gtaaaagacg cagatgtatt tattggagtt tctgtagcta atgcgctgtc acaagatatg
     5581 gttaagagta tggcagataa tccaattata tttgcaatgg ctaatccaaa tcctgaaata
     5641 atacctgatg atgccaaagc agcaggtgca cgcgttgttg gtacaggacg ttcagactat
     5701 cctaaccaaa ttaataatgt attagctttc cctggtattt ttagaggtgc attagaggtt
     5761 gaagctacac atataaatga agaaatgaaa aaagcagctg tagaagcgat tgctgattta
     5821 attgatagtt ctgaattaaa tgaagactac tgtatcccag gaccgtttga taaacgtgta
     5881 gcgccatcag ttgctcgtaa tgttgctaaa gcggcaatgg aatctggagt agctaggatt
     5941 gaagttgatc cgcaagatgt gtatgataaa acaatgaaac ttacagattt acaataaaaa
     6001 taattaacgt ttaattaaag tgcttaaaag ttagcaataa agagtcatta aaatgatgaa
     6061 aatgaaagtt tatgataaac attcaacagt caaacgaata taaatcaaat aaatttaaac
     6121 ccgtttttaa ctggtcaagt tcagtttaag gctctaaatg gttagaacag aggttatttg
     6181 gaggttttcc tatgtttaaa gattttttta atcgaacaaa gaaaaagaaa tatcttacag
     6241 tacaagactc taaaaataat gatgtgcctg caggtattat gactaagtgt ccaaagtgta
     6301 agaaaattat gtacacaaaa gaattagctg aaaatttaaa tgtgtgcttt aattgtgatc
     6361 atcatattgc tttaactgcg tataaacgta tagaagcaat ttctgatgaa ggatcattta
     6421 cagaattcga taagggaatg acctctgcga atccattaga ttttccaagt tatttagaaa
     6481 aaattgaaaa ggaccaacaa aagacaggtc ttaaagaagc agtagtgact ggtacagcgc
     6541 aactagatgg tatgaaattt ggcgttgctg tcatggattc acgttttaga atgggaagta
     6601 tgggatcggt tattggtgaa aagatatgtc gcatcattga ttactgcact gagaaccgtt
     6661 taccatttat tcttttctct gcaagtggtg gcgcacgtat gcaagaaggt attatttcct
     6721 tgatgcaaat gggtaaaacc agtgtatctt taaaacgtca ttctgacgct ggactattat
     6781 atatatcata tttaacacat ccaactactg gtggtgtatc tgcaagtttt gcatcagttg
     6841 gtgatataaa tctaagtgag ccaaaagcat tgataggttt tgcaggtcgt cgagttattg
     6901 aacagacaat aaacgaaaaa ttgccagatg atttccaaac tgcagaattt ttattagagc
     6961 atggacaatt ggataaagtt gtacatcgta atgatatgcg tcaaacattg tctgaaattc
     7021 taaaaatcca tcaagaggtg actaaataat gttagatttt gaaaaaccac tttttgaaat
     7081 tcgaaataaa attgaatctt taaaagaatc tcaagataaa aatgatgtgg atttacaaga
     7141 agaaattgac atgcttgaag cgtcattgga acgagaaact aaaaaaatat atacaaatct
     7201 aaaaccatgg gatcgtgtgc aaattgcgcg tttgcaagaa agacctacga ccctggatta
     7261 tattccatat atctttgatt cgtttatgga actacatggt gatcgtaatt ttagagatga
     7321 tccagcaatg attggtggta ttggcttttt aaatggtcgt gctgttacgg ttattggaca
     7381 acaacgtgga aaagatacaa aagataatat ttatcgaaat tttggtatgg ctcatccaga
     7441 aggttatcga aaagcactac gtttaatgaa acaagctgaa aaattcaatc gtccaatctt
     7501 tacatttata gatacaaaag gtgcatatcc tggtaaagct gctgaagaac gtggacaaag
     7561 tgaatctatt gcaacaaatt tgattgagat ggcttcacta aaagtaccag ttattgcgat
     7621 tgtcattggt gaaggtggca gtggaggtgc cctaggtatt ggtattgcca ataaagtatt
     7681 gatgttagag aatagtactt actctgttat atctcctgaa ggtgcagcgg cattattatg
     7741 gaaagacagt aatttggcta aaattgcagc tgaaacaatg aaaattactg cccatgatat
     7801 taagcaatta ggtattatag atgatgtcat ttctgaacca cttggcggtg cacataaaga
     7861 tattgaacaa caagctttag ctattaaatc agcgtttgtt gaacaattag attcacttga
     7921 gtcattatca cgtgatgaaa ttgctaatga tcggtttgaa aaattcagaa atatcggttc
     7981 ttatatagaa taatcaactt aagcattttt atgttaaatc gttactgggt tttaccataa
     8041 attgaagtac attaaaacaa taattcaata tttagatact gaattttaac taagattagt
     8101 agtcaaaatt gtggctacta atcttttttt aattaagtta aaataaaatt caatatttaa
     8161 aacgtttaca tcaattcaat acattagttt tgatggaatg acatatcaat ttgtggtaat
     8221 ttagagttaa agataaatca gttatagaaa ggtatgtcgt catgaagaaa attgcagttt
     8281 taactagtgg tggagattca cctggaatga atgctgccgt aagagcagtt gttcgtacag
     8341 caatttacaa tgaaattgaa gtttatggtg tgtatcatgg ttaccaagga ttgttaaatg
     8401 atgatattca taaacttgaa ttaggaacag ttggggatac gattcagcgt ggaggtacat
     8461 tcttgtattc agcaagatgt ccagagttta aggagcaaga agtacgtaaa gttgcaatcg
     8521 aaaacttacg taaaagaggg attgagggcc ttgtagttat tggtggtgac ggtagttatc
     8581 gcggtgcaca acgcatcagt gaggaatgca aagaaattca aactatcggt attcctggta
     8641 cgattgacaa tgatatcaat ggtactgatt ttacaattgg atttgacaca gcattaaata
     8701 cgattattgg cttagtcgac aaaattagag atactgcgtc aagtcacgca cgaacattta
     8761 tcattgaagc aatgggccgt gattgtggag atctagcatt atgggctgga ttatcagttg
     8821 gtgctgagac aattgtagtt ccagaagtga aaacagatat taaagaaata gctgataaaa
     8881 ttgaacaagg tattaaacgt ggtaagaaac actcaatcgt tcttgtagca gaaggttgta
     8941 tgactgcgca agattgtcaa aaagaattat cacaatacat caatgttgat aatagagtgt
     9001 ctgtgttagg tcacgttcaa cgtggtggta gcccaacagg tgcggataga gttttagcat
     9061 cacgattagg tggatatgcg gtagacttat taatgcaagg tgaaacagct aaaggtgttg
     9121 gaattaagaa caataaaatt gtagcaacat cttttgatga aatttttgat ggtaaagatc
     9181 ataaatttga ttatagtcta tatgaacttg ctaacaagtt atctatataa gatttcagga
     9241 ggaattataa aatgagaaaa actaaaattg tatgtacaat tggaccagct tcagaatcag
     9301 aagaaatgat tgagaaatta atcaatgctg gtatgaacgt tgcacgatta aacttttcac
     9361 atggtagtca tgaagagcat aaaggtagaa ttgatacaat tcgtaaagta gctaaaagat
     9421 tagacaaaat tgtagcaatt ttattagata cgaaaggtcc agaaattcgt acgcataata
     9481 tgaaagacgg tatcattgaa cttgaacgtg gtaacgaagt tattgttagc atgaatgaag
     9541 ttgaaggaac acctgaaaag ttctctgtaa cttatgaaaa cttaattaac gatgttcaag
     9601 taggttcata cattttactt gatgatggct taattgaatt acaagttaaa gatattgacc
     9661 atgctaaaaa agaagttaaa tgtgatattt taaactctgg tgagcttaaa aacaaaaaag
     9721 gtgttaactt acctggtgta agagttagtt tacctggtat tacagaaaaa gatgctgaag
     9781 atatccgttt cggtattaaa gaaaatgttg acttcattgc agcaagtttc gtacgtcgtc
     9841 ctagtgatgt tttagaaatt cgtgaaattt tagaagaaca aaaagctaac atttcagtat
     9901 tccctaaaat tgaaaaccaa gaaggtattg ataatattga ggaaattctt gaagtgtctg
     9961 atggtttaat ggttgcacgt ggtgacatgg gtgttgaaat tccacctgaa aaagtaccaa
    10021 tggttcaaaa agatttaatc agacaatgta acaaattagg taaaccagtt attacagcta
    10081 cacaaatgtt agattctatg caacgtaacc cacgtgctac acgtgcagaa gctagtgacg
    10141 ttgccaacgc aatctatgat ggtacagatg cagtaatgtt atctggtgaa actgctgctg
    10201 gtttatatcc tgaagaagct gttaaaacaa tgagaaatat tgctgtatca gctgaagcag
    10261 cccaagatta caaaaagtta ttgtcagatc gtactaaatt agttgaaact tcattagtga
    10321 atgctatcgg tatttcggtt gcacatacag ctttaaactt aaatgttaaa gcaattgtag
    10381 ctgctactga aagtggttca acggcacgta ctatctctaa atatcgtccg cattcagaca
    10441 ttattgcagt gactccaagt gaagaaactg cacgtcaatg ttcaattgtt tggggtgttc
    10501 aacctgtagt taaaaaagga cgtaagagta cagatgcatt gttaaacaat gcagttgcaa
    10561 cagctgttga aactggtaga gtaactaatg gtgatttaat cattattact gctggtgtac
    10621 caactggtga aactggaact actaatatga tgaaaatcca cctagttggt gacgaaattg
    10681 ctaatggtca aggtattgga cgtggatcag ttgttggtac tacgttagtt gctgaaactg
    10741 ttaaagattt agaaggtaaa gatttatctg acaaagttat cgttacaaac tcaatcgatg
    10801 aaacgtttgt accttatgta gaaaaagcgt taggcttaat tacagaagaa aatggaatta
    10861 catcaccaag tgcaattgtt ggtttagaaa aaggtattcc aacagttgta ggtgtagaaa
    10921 aagctgttaa aaacataagc aataacatgt tagttacgat tgatgctgct caaggtaaaa
    10981 tctttgaagg atatgcaaac gtactataat ttataaaaaa acgtctttcc atttatcaac
    11041 aatggaaagg cgttttttgg ttcatctggt attttatgac gtaattaata ggatatttga
    11101 taatgataat gtatgaatag caatctatat aaatgtttat atcttttata catgtacatt
    11161 ttcaccttta aactttcact catattaatt tggaaagtta ttataaaata gaagtatgga
    11221 tgtatctcta aaatgataca ttattcaata gatgagaaaa agtattgagg caagtacgca
    11281 atttaatatt ataagttgca tataaaaacc cctgttgaac tttaatatat tcaacagggg
    11341 taaaacttgt ttttagttat ttgatttagc agcgatacgt ttatatctta agtacataaa
    11401 tcctaatagt ataaaccaaa tcggaataaa gtatatcgca cgtcttgttt caacgttgat
    11461 aaacaatagt ccaaacacaa atataaagaa tacaaaaatg agatagccca tatattttcc
    11521 gccaaataat ttatacgtag cctttttatg aagatctgga tttttacgac tataattgat
    11581 ataagcgatg ataatcagtc cccaaacaac taagaataat actgtagaga ttgttgtcac
    11641 atacgtaaat accttagtag catcagggaa aatatagttt aataatgctg ccacgagtaa
    11701 caatgctgat gaagcaaaga tcgctacatg tggaacgcca tacttattcg tcttagagaa
    11761 attcggtggt gcttgttgtt gacttgataa accgaacagc atacgactat tcgagaaaat
    11821 accactgtta catgatgaag cagcagcagt tagtactaca aaattaatca agcctgcagc
    11881 aaatggaatt ccaattaatg cgaataattt tacgaatgga ctgttatcag gatcaacttg
    11941 ttgccaagga ataatcgaca tgataaccgc tagagcaccg acataaaata ttaaaattct
    12001 aataggaaca ctattaatcg cttttggtat tgttttaaca ggatctttcg tttcacctgc
    12061 cgtaacacca atcatttcga ttcccacgaa tgagaataag gccatttgga atgacataaa
    12121 gaatccagat gctcctttag ggaatacacc gtgctcgtat aggttcgtga aactcgcatg
    12181 tccaaattga gttttatatg caaatagaat cataacgaaa cctacgacaa ttaaaccaat
    12241 gattgttgct attttaatga tagagaacca aaattctaac tctccaaata gtctcgcact
    12301 caataagtta aaagacatta ataacaatac gcaaaaaagt gcactaatcc agtttggaat
    12361 ttctgggaac cagaagctta catactttgc tacagctgtt acttcagcca tacctgtaat
    12421 aatccaacag aaccagtatg tccaaccggt tacgaatcct gcaaaaggcc caatgtatgt
    12481 attggttaca tctgcgaaag atttaaattc agtattttga atgatgattt cacctaaacc
    12541 tcg
//
LOCUS       DAPIDM010000068        12441 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000068 DAPIDM010000000
VERSION     DAPIDM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.068"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            144..1310
                     /gene="norA"
                     /locus_tag="RJE42_001637"
     CDS             144..1310
                     /gene="norA"
                     /locus_tag="RJE42_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDV5877054.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADVSPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSVVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPAILTLVLAFGL
                     SAFETLYSLYTSYKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     IYSVIVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGDRQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKQQDL"
     gene            1608..2060
                     /locus_tag="RJE42_001638"
     CDS             1608..2060
                     /locus_tag="RJE42_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877055.1"
                     /translation="MTVKNLFLGFVAIILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            2234..2716
                     /gene="ybaK"
                     /locus_tag="RJE42_001639"
     CDS             2234..2716
                     /gene="ybaK"
                     /locus_tag="RJE42_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDV5877056.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAERIQAN
                     VKTVFKTLVLENAKHEHFVFVIPVSETLDMKKAAAMVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            2966..3730
                     /locus_tag="RJE42_001640"
     CDS             2966..3730
                     /locus_tag="RJE42_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV5877057.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMVI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNKESLKEYQHKYHFIGGTL"
     gene            3727..4647
                     /gene="pfkB"
                     /locus_tag="RJE42_001641"
     CDS             3727..4647
                     /gene="pfkB"
                     /locus_tag="RJE42_001641"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV5877058.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIADTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSAQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLSYHPLFIKPNKDELEVMFNTTVNSDEDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            4653..6617
                     /locus_tag="RJE42_001642"
     CDS             4653..6617
                     /locus_tag="RJE42_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV5877059.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLTDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVAAVEANQDTNGSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVET
                     NGSSGIKNHLTEQDIKNATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKA
                     LDTSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVF
                     LFGANSFDPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGG
                     MLAISGGSGFIGGIIAGFLAGYLTQGVKVITRKLPQALEGLKPTLIYPLLTVTATGLL
                     MIYVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGA
                     LIEGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAI
                     PFAAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALV
                     VGTLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            6925..8106
                     /gene="nagA"
                     /locus_tag="RJE42_001643"
     CDS             6925..8106
                     /gene="nagA"
                     /locus_tag="RJE42_001643"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV5877060.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAEIDN
                     DTTNKIQVIDAKGHHVVPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDTAIINIAKYEVEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDRLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            8327..9676
                     /locus_tag="RJE42_001644"
     CDS             8327..9676
                     /locus_tag="RJE42_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV5877061.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLDTIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEPRPTIGQSDEDEKSE"
     gene            9896..10735
                     /locus_tag="RJE42_001645"
     CDS             9896..10735
                     /locus_tag="RJE42_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV5877062.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            10867..11850
                     /gene="csbB"
                     /locus_tag="RJE42_001646"
     CDS             10867..11850
                     /gene="csbB"
                     /locus_tag="RJE42_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDV5877063.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEIQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVARQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(11923..>12441)
                     /gene="saeS"
                     /locus_tag="RJE42_001647"
     CDS             complement(11923..>12441)
                     /gene="saeS"
                     /locus_tag="RJE42_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDV5877064.1"
                     /translation="MTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEV
                     NFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIAISDEG
                     IGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTT
                     MTVTLHKLDITS"
BASE COUNT         4345 a         1684 c         2415 g         3997 t
ORIGIN      
        1 aaagaaaaaa atattgtcaa atgtagcaat gttgtaatac aatatagaaa ctttttacga
       61 atatttagca tgaattgcaa tttgtcgtgg aaaagaagaa taacagcttt aagcatgaca
      121 tggagaaaaa aagaggtgag catatgaata aacagatttt tgtcttatat tttaatattt
      181 tcttaatttt tttaggtatc ggtttagtaa taccagtctt gcctgtttat ttaaaagatt
      241 tgggattaac tggtagtgat ttaggattac tagttgctgc ttttgcgtta tctcaaatga
      301 ttatatcgcc gtttggtggt acgctagctg ataaattagg gaagaaatta attatatgta
      361 tagggttgat tttattctca gtgtcagaat ttatgtttgc agttggccac aatttttcgg
      421 tattgatgtt atcaagagtg attggtggta tgagtgctgg tatggtaatg ccgggtgtga
      481 caggtttgat tgcagatgtt tcaccaagcc atcaaaaagc aaaaaacttt ggctacatgt
      541 cagcgattat caattctgga ttcattttag gaccagggat tggtggattt atggcagaag
      601 tttcgcatcg tatgccattt tattttgcag gtgcattagg cattttagct ttcataatgt
      661 cagttgtatt gattcatgat ccgaaaaagt ctacgacaag cggcttccaa aaacttgagc
      721 cccaattatt aacaaaaatt aattggaaag tctttattac gccagcaatt ttaacgctcg
      781 tcttagcgtt cggtttatcg gcatttgaaa cactgtattc tttatataca tcgtataaag
      841 taaattattc acctaaagat atttcgattg cgattacggg tggcggtatc tttggtgcac
      901 ttttccaaat ttatttcttt gataaattta tgaaatactt ctctgagtta acatttattg
      961 catggtcatt aatatattca gttattgtat tagtgctatt agttattgcc gatggttact
     1021 ggacaattat ggtaattagc tttgttgtct ttatcgggtt cgatatgata agacctgcta
     1081 ttacaaatta tttctcaaat attgctggtg atagacaggg atttgcaggt gggttaaact
     1141 cgacattcac tagtatgggt aatttcatag gtcctttaat cgcaggtgcg ttatttgatg
     1201 tacacattga agcaccaatt tatatggcta taggtgtgtc attagcaggt gttgttattg
     1261 ttttaattga aaagcaacat agagctaagt taaaacaaca agatttgtaa tatcgcacat
     1321 ggttgtcatt cgattcatgt atttcgacaa ctaaagcatt tcaatataag catcgatttt
     1381 cttacaatta tagtgaagaa aagtcgatgc tttaaattta taaagattaa aaacttttaa
     1441 atgttttagt ctttatattt aaatgttata tgtaacaaaa aatgattttg agtaataaac
     1501 gtgttacaaa tattacattc tttttaaatt gcaatccaca tacctaattc attaacgtta
     1561 atgtgttaag atgataaaaa aatgagtaag gaaatgtggg taaggggatg acagtaaaaa
     1621 atttattttt aggctttgtt gctataatat taaccgtttg tttaattggt ttattaatat
     1681 tagcaacaaa tgaagatgcg cttgctaagg tacataaaac gattaatacg cttaacgcga
     1741 taaatgtatc aactgaagat acttataaaa agaaaatgga tattctcaat attcatactg
     1801 ctaaagcgtc tgaagtgaat gaaaatgtga aaaagcaaaa tcattttaag catcgtgtga
     1861 atgcaaataa atcaaattct tttaacgaac aagagtgcca agttattgct gatcgttatg
     1921 cagataagca tatcaatgat aattatggtt tagaaagaat ttctaagaca aatcatggat
     1981 ataattatgt ttattccaat gataattcaa ctagtaagca acatgtaagt atttcaaatc
     2041 aaggcataat aacgaaataa tagatggaac agtgtattct aattggatat actgttttta
     2101 ttttgcaata ttttaattta aaaaggtgaa atcaacttat aaaatgatgt aaatgttatg
     2161 tcaaaatcaa ctaatccgta atgtatttta aaatgttaat atagttctga agaagtataa
     2221 atgaggtgtt gaaatggcta aaaataagaa aacgaacgcg atgcgtatgc ttgatcgtgc
     2281 aaaaattaaa tacgaagttc atagctttga ggtaccagaa gaacatttat ctggccaaga
     2341 agtcgcagaa cgcatacaag caaatgttaa aacagtattt aaaacacttg ttctagaaaa
     2401 tgcaaaacat gaacattttg tatttgttat cccagtaagt gaaactttag atatgaaaaa
     2461 ggcagctgct atggttggag agaagaaatt gcaacttatg cctttagata atttaaaaaa
     2521 tgtaacggga tacattcgtg gtgggtgttc acctgttggc atgaaaacat tgtttccaac
     2581 agtcgttgac aaatcctgtg aaaattatag tcatatcagt gtgagtggtg ggcttcgaac
     2641 aatgcaaatc acgatagctg ttgaggattt gattacaata actaaaggca aaattggagc
     2701 agttatccat gaatgattaa taacaacaaa aagtatgggg caagattagg agtgtgacag
     2761 agatgaaatt tttataaaat tcatttctgc cacactcctt tttgattgaa ttagcatttt
     2821 acgatcataa acagtcatga taattgagta tttgaacata aaaatgtaat tttatcgtca
     2881 caatttgatt gtttgtgatt gtttttggta atttatgatt gaaaagtgaa agcgtactca
     2941 ttataataca gagtgagatg gggtgatgat gataattaca gaaaaaagac acgagttaat
     3001 attagaagaa ctttcgcaca aagatttttt gactttacaa gaattaatag atcgaactgg
     3061 ttgcagtgct tcaacaatac gaagagattt atccaagcta caacaattag ggaaattgca
     3121 acgtgtgcat ggtggtgcaa tgttaaaaga aaatcgtatg gttgaggcga atttaactga
     3181 aaaattagca acgaatctcg atgaaaagaa aatgattgct aaaatagcag ctaatcaaat
     3241 caacgataat gaatgcttat ttatcgatgc tggttcttct acattggagc taattaaata
     3301 tattcaagcg aaagatatca ttgtggtaac caatggttta actcatgtag aagctttact
     3361 taaaaaaggt attaaaacaa ttatgctagg tggtcaagtt aaagaaaata cacttgctac
     3421 gattggttca agtgccatgg tgatattaag acgatattgt ttcgataaag cttttatcgg
     3481 gatgaatgga ttagatattg aacttggatt aactactccc gatgagcaag aggcattagt
     3541 taaacaaaca gcaatgtcat tagccaatca atcatttgta cttatagatc attctaagtt
     3601 taataaagta tattttgctc gtgtaccttt gctagaaagt acgacaatca tcacatctga
     3661 aaaagcttta aataaagaat cgttaaaaga ataccaacat aagtatcact ttataggagg
     3721 gactttatga tttatacagt gactttcaat ccttcaattg actatgtcat ttttacgaat
     3781 gattttaaaa ttgatggttt gaacagagca acagcaacat ataaattcgc cgggggaaaa
     3841 ggtattaatg tctcacgtgt tttaaagaca ttggatgttg agtcaactgc tttgggattt
     3901 gcaggtggat ttcctgggaa attcattgca gatacattaa ataacagtgc aattcaatcg
     3961 aattttattg aagttgatga agatacacgt attaatgtga aattaaaaac aggacaagaa
     4021 acagaaatca atgcaccggg tcctcatata acgtcagcac aatttgaaca attgttacaa
     4081 caaattaaaa atacaacaag cgaagatata gttattgttg ctggaagtgt accaagtagt
     4141 attccaagcg atgcgtatgc gcaaattgca caaattacag cacagacagg tgctaaatta
     4201 gtagtcgacg ctgaaaaaga attggctgaa agcgttttat catatcatcc actatttatt
     4261 aaacctaata aagacgaatt agaagtgatg tttaatacaa cagtgaactc agacgaagat
     4321 gttattaaat atggtcgttt gttagttgat aaaggtgcac aatctgttat tgtctcactt
     4381 ggcggtgatg gtgctattta tattgacaag gaaatcagta ttaaagcagt taatccacaa
     4441 gggaaagtgg ttaatacagt tggctctggt gatagtacag ttgcaggcat ggtggctgga
     4501 attgcttcag gtttaacgat tgaaaaagca ttccaacaag cagtcgcatg cggtactgcc
     4561 acggcatttg atgaagactt agcaacacgg gacgctatag aaaaaataaa atcacaagtt
     4621 acgattagcg tacttgatgg ggagtgaaaa taatgagagt aacagagtta ttaacaaaag
     4681 atacaatagc aatggattta atggcaaatg acaaaaatgg tgttattgat gaattagtaa
     4741 atcaattaga caaagcaggt aaattaactg atgtcgcgtc atttaaggaa gcgattcaca
     4801 atcgagaatc acaaagtacg actggtatcg gcgaaggtat tgctattcca catgcgaaag
     4861 tagcagcagt taagtctcca gctatcgcat tcggtaaatc taaagaaggt gtagattacc
     4921 aaagtttaga tatgcaacca gctcatttat tctttatgat tgcagcgcca gaaggtggtg
     4981 cccaaacaca tttagatgct ttagctaaac tatctggtat tttaatggat gaaaatgtac
     5041 gtgaaaaatt attacatgca tcgtctccag aagaagtgtt agcaattatt gatcatgctg
     5101 atgatgaagc gacaaaagaa gaagaggcag aagctgaagc acaacaagtt gctgctgtag
     5161 aagcaaatca agacactaat ggatcaaacg agccatatgt attagctgta actgcttgtc
     5221 caacaggtat tgcacacaca tatatggcac gtgatgcatt gaaaaagcaa gcggataaaa
     5281 tgggcattaa aattaaagtt gaaacgaacg gttcaagtgg tattaaaaac catttaacgg
     5341 aacaagatat taaaaatgca acaggtatca ttgttgctgc tgatgttcat gttgagacgg
     5401 atcgcttcga tggtaaaaat gtcgtagaag taccagtcgc agatggtatt aagcgcccag
     5461 aagaattaat taataaagca ttagatacaa gtcgtaaacc ttttgttgcc cgtgatggtc
     5521 aaagaaaagg taactcaaat gacagtcaag aaaaattaag cccaggtaaa gctttctata
     5581 aacacttaat gaacggtgtt tctaacatgc taccacttgt aatatctggt ggtattttaa
     5641 tggcaatcgt gtttttattt ggtgcaaact catttgatcc aaaaagctca gagtacaatg
     5701 cgtttgcaga gcagctttgg aacattggta gtaaaagtgc attcgcgtta atcattccaa
     5761 ttttatctgg attcattgca cgtagtattg cggataaacc tggattcgct tcaggtcttg
     5821 taggtggtat gttagcaatt tcaggtggtt caggatttat tggtggtatt attgcaggtt
     5881 tcttagcagg ttacttaaca caaggtgtta aagtgattac acgtaagtta ccacaagcat
     5941 tagaaggatt aaaaccaaca ttaatttacc cactattaac agtgacagct acaggcttat
     6001 tgatgattta tgtctttaat ccaccagcat cttggttaaa tcatttatta ttagatggat
     6061 taaataattt atcaggttct aatattgtat tattaggttt agttattggc gctatgatgg
     6121 cgattgatat gggcggtcca ttcaacaaag cggcatatgt ttttgcaaca ggtgcgttga
     6181 ttgaaggtaa tgcagcacca attacagctg caatgattgg tggtatgatt ccaccgttag
     6241 caattgcgac agcgatgtta attttcagac gtaaatttac aaaagaacaa cgtggttcaa
     6301 ttatccctaa ctatgtgatg ggtatgtcct ttattacaga aggtgcgatc ccattcgcgg
     6361 ctgccgatcc attacgtgtt atcccttcaa tgatgattgg gtcaggtata ggtggcgcaa
     6421 tcgctttagg tttaggttct cgaattactg cgccacacgg cggtattatc gtaattgttg
     6481 gtacggatgg tgcacattta ctacaaacac ttattgcact tgtagtaggt acattagttt
     6541 cagcattaat ttatggactt attaaaccaa agttaactga aacagaaatc gaagcttcaa
     6601 aatcaatgga cgagtagttt taatgattta aaatgattgt tagcaaagag cttcatatta
     6661 agttgtaagt tcaatgaata tatgttagtt ttatatatcg tgttaacgat agcttttaca
     6721 aagttataaa aacactttct attaattcag tttttatgaa ttgatatgaa ggtgttttta
     6781 tttttagata aatgaatgaa gaaataaaca ccacaaatgt atagactttt ttaatatttt
     6841 gcaaaaagtt atgccaaacg aagcagatat agtaaaatat gagtgtctta aagtgaaaat
     6901 ttataaataa agaagggttt atacgtgtca gaattaatta tatataacgg caaagtttat
     6961 actgaagatg gcaaaatcga taatggttac attcatgtga aagatggaca gattgttgca
     7021 attggagaag tggatgataa agcagagatt gataatgata cgacaaataa aattcaagtg
     7081 attgatgcaa aaggtcatca tgtagtgcca ggttttatcg atatacatat tcatggtggt
     7141 tatggacagg atgcaatgga tgggtcatac gatggcttaa aatatctatc cgaaaatttg
     7201 ttgtctgaag ggacgacatc gtacttggcc actacaatga cgcaatcgac tgataaaata
     7261 gatactgcaa ttataaatat tgctaaatat gaagtggagc aagatgttca caatgcagcg
     7321 gaaattgtag gtatacattt ggaaggacca tttatatctg aaaataaagt tggtgctcaa
     7381 catccgcaat acgtagtacg cccatttatc gataaaatta aacattttca agagactgct
     7441 aacggattaa taaagattat gacgtttgca cctgaagttg aaggtgcaaa agaagcgctt
     7501 gaaacgtata aagatgacat tattttttca attggtcata cagtggcaac atacgaagaa
     7561 gcagttgaag ctgtcgagcg aggagctaaa catgtcacgc atttatataa tgcagcgacg
     7621 ccattccaac atagagaacc aggtgttttt ggagcagcat ggttgaatga tgctttacat
     7681 accgaaatga ttgttgatgg cacacattct catccggcat cggttgcaat tgcttaccgt
     7741 atgaaaggta atgaacgttt ttatttaatt accgatgcaa tgcgtgcaaa aggtatgcct
     7801 gaaggagaat atgatttggg tggccaaaaa gtaactgttc aatcgcaaca agcacgtctt
     7861 gcaaatggtg cgcttgctgg tagtatttta aaaatgaatc atgggttacg taacttaata
     7921 tcatttacag gtgatacatt agatcgttta tggcgtgtaa caagtttaaa tcaagccatt
     7981 gcattaggta tcgatgatag aaaaggtagt attaaagtaa ataaggatgc agatcttgtt
     8041 attctagatg atgacatgaa tgtaaaatct acaataaaac aaggtaaggt tcacacattt
     8101 agctaataga taatcataat taaatgtatg caatagattt aatctgttaa cataagcact
     8161 ttatattatg ataaaataga agcaataaca tttttttctg ggggtgtcta aatgggaagg
     8221 cgataacatg tagttgtaat ttaagtcata gtgataaatt tgaatgcgtg ttacccatga
     8281 gtgacacata taacatggag gtgaatccct agaaataggg aattaattgg aaacttcgac
     8341 cataattagt ttgattatat ttattctatt aattgcatta accacagtat ttgttggttc
     8401 agaatttgca ttagtaaaaa ttagagcaac aagaattgaa cagctagcag atgaaggaaa
     8461 taagcctgct aaaatagtaa aaaagatgat tgctaatcta gattattatc tttctgcttg
     8521 tcagttaggt ataacagtaa catctttagg gttaggttgg cttggtgaac caacgtttga
     8581 aaagctatta cacccgatat ttgaagcaat caatttacca actgcattaa cgacgacgat
     8641 ttcgtttgca gtatcattta taatcgttac gtatttgcat gtagtacttg gtgaattagc
     8701 gcctaaatct atagcgattc aacatactga aaagcttgct ttagtatatg caagaccatt
     8761 gttctatttc ggtaacatta tgaaaccatt gatttggctg atgaatggtt ctgcacgtgt
     8821 tattattaga atgtttggtg taaatcctga tgcccaaacc gatgcaatgt cagaagaaga
     8881 aatcaaaatt attattaaca atagttataa tggtggagaa atcaaccaaa ctgaattggc
     8941 atatatgcaa aatatctttt cattcgatga aagacatgca aaagatatta tggtacctag
     9001 aactcaaatg attacactaa atgaaccttt taatgtagac gaattacttg atacaataaa
     9061 agaacatcaa tttacacgtt atccaattac tgatgatggt gataaagacc atattaaagg
     9121 atttatcaat gttaaagagt ttttaactga atatgcttct ggaaaaacaa ttaaaattgc
     9181 aaactatatt catgaattgc caatgatttc agagacaaca cgtatcagtg atgcattaat
     9241 tagaatgcaa cgtgaacatg tacatatgag tcttattata gatgaatatg gtggaacggc
     9301 aggtatttta acgatggaag atattttaga agaaatcgtt ggggaaattc gtgatgaatt
     9361 tgatgatgat gaagtgaatg atatcgttaa aattgataat aagacattcc aagtaaatgg
     9421 tagagtacta ttggatgatt taactgaaga gttcggtata gaatttgatg actctgagga
     9481 tattgatacg ataggtggat ggttacaatc tcgtaatacc aacttacaaa aagatgatta
     9541 cgtggataca acttatgatc gctgggttgt ttcagaaatc gataaccacc aaattatttg
     9601 ggtaatgtta aactatgaat ttaatgaacc gagacctact atcggacagt ctgatgaaga
     9661 tgaaaaatca gaatagatag taatatataa accaactaag aatgatttaa ttcattcttg
     9721 gttggttatt ttttgactaa aattaaagaa aaatgaaaat agtcttgaac tcaatatcta
     9781 taatgattta atgaatattt tttattgaaa gcgataattc atattaattg agtttgttga
     9841 aaaatttagg gtaatgtaaa gatataaaag atacatagat tggagagata taaagatgtt
     9901 gaatgagata caaatattaa ataatggata cccaatgcct tcagttgggt taggtgttta
     9961 taaaatctct gacgaagata tgactaaagt tgtaaatgct gcaattgacg caggctatag
    10021 agcgtttgat acagcatact tttatgataa tgaggcttca ctaggacgag cattaaagga
    10081 taatggcgta gatagagaag atttgtttat aacgacgaag ttatggaatg actatcaagg
    10141 ttatgagaaa acattcgaat atttcaacaa atcgattgaa aatttacaaa ctgattatct
    10201 tgatttattt ctaatacatt ggccttgtga agcagatggt ctatttttag aaacatataa
    10261 agctatggaa gaactttacg agcaaggtaa ggtaaaagca ataggtgtat gtaattttaa
    10321 tgttcatcat ctagaaaaat taatggctca atcaagtatc aaaccaatgg tgaatcaaat
    10381 tgaggtacat ccatatttta accaacaaga attacaagaa ttttgtgatc gtcacgatat
    10441 taaagtgact gcatggatgc ctttgatgag aaatagagga ctactagacg accctgtcat
    10501 tgttaaaatt gctgaaaaat atcataaaac accagcacaa gttgtattgc gttggcattt
    10561 agcacacaat agaattatta ttccaaaatc tcagacacct aaacgcattc aagaaaatat
    10621 agatatttta gattttaatt tagaattaac agaagtagct gaaattgatg ctttaaatag
    10681 aaatgcaaga caaggtaaaa atccagatga tgtgaaaatt ggggatttaa aataactgga
    10741 tgttaaattt tacgtttatg aatgtctttt aatgtgtaca ttaaaataaa tgagttggtt
    10801 tttactattt gataaaacaa tactcaggta cattcaaaat cttttaaata aaaaggatgg
    10861 acatagatga aaattagagt cgtcattcct tgttttaatg aaggggaagt cattacacaa
    10921 acacatcaac aattaactga aatactttca caagatagta gtgtgaaagg ctatgattat
    10981 aatatgcttt ttatagatga tggtagtacg gataccacta tagatgaaat acaacatctt
    11041 gccacaatag atagacatgt cagctttatt tcttttagca gaaattttgg aaaagaagca
    11101 gctatgattg caggttacca gcatagtact gaatttgatg cagtcatcat gatagattgt
    11161 gatttgcaac atccacctga atatattccg aaaatggttg aaggttttat ggaaggctat
    11221 gatcaagtga ttgcaaagcg tgatagaagt ggtgaaaatt ttagtcgcaa aacattaagc
    11281 catttgtact ataagttagt taattgcttt gtagaagaag tacaatttga tgatggtgtt
    11341 ggtgatttta gacttttaag ccaaagagct gttaaatcca ttgcatcact tgaagaatat
    11401 aatcgatttt caaaagggtt atttgaatgg ataggatata atactaaagt gtttacgtat
    11461 caaaatgttg cgagacaaaa aggagaatct aagtggtcct ttaaaaagtt atttaattat
    11521 ggtattgatg gcttgatttc ctttaatagt aaacctttga gaatgatgat atatcttggc
    11581 ttatttattt tttcaattag cgtgttatat attatctatt tattcatcaa tattatgatt
    11641 tctggtgtta atatcccagg atatttttca acgattgcag ctattttatt attaggcggc
    11701 atacagttaa tttcaattgg tgttgtaggt gaatatattg gcaggatata ttatgaagtt
    11761 aaggcacgtc ctaaatatat tattcaagct acaaatcttt caagtattga aaatgatgag
    11821 aaggataccc ataaagttta ctctaaataa acaaataaaa gaagccctca tcaatgagag
    11881 cttcttttta gtctttgcat tttattttat aaataaatcg gattatgacg taatgtctaa
    11941 tttgtgtaat gttacagtca tcgtagttcc tacatctata tcactgctta cactgatttt
    12001 tgcgttattt tgttgcgcga gttcattagc tatatataag cctaatccag aaccacccgt
    12061 ttttgtatta cgagagtttt ctactctgaa tgttcgttcg aatatacgtt cttgtagttc
    12121 tggtataatg ccaatacctt catcgctaat agcaatgtcg atagtatctt gatctttgtt
    12181 ttcactaata ttaatatcaa tgcgactacc aacatttgaa aattttagcg cattatcaag
    12241 taagtttgtt aaaatacgct caagtggcgt tcgatattga taaaatgcat caatttcgct
    12301 acagaaattc acttctaatg tgcggttctc atgtttgata cgttgctcat atggttgcaa
    12361 tattgataca agtaattggt ctagttgtat taattctggg ggatatgttt tacctgtatt
    12421 taaagtgata atatgagtca t
//
LOCUS       DAPIDM010000069        12358 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000069 DAPIDM010000000
VERSION     DAPIDM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.069"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(486..1826)
                     /gene="glnA"
                     /locus_tag="RJE42_001648"
     CDS             complement(486..1826)
                     /gene="glnA"
                     /locus_tag="RJE42_001648"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDV5877065.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1845..2213)
                     /locus_tag="RJE42_001649"
     CDS             complement(1845..2213)
                     /locus_tag="RJE42_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDV5877066.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2457..3695)
                     /locus_tag="RJE42_001650"
     CDS             complement(2457..3695)
                     /locus_tag="RJE42_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV5877067.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGISERTKV
                     IAIQRSKGYDQRPSIPLDEIEQVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVKQLKEQGLI"
     gene            complement(3714..4952)
                     /gene="hflX"
                     /locus_tag="RJE42_001651"
     CDS             complement(3714..4952)
                     /gene="hflX"
                     /locus_tag="RJE42_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV5877068.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDEAIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5064..5540
                     /locus_tag="RJE42_001652"
     CDS             5064..5540
                     /locus_tag="RJE42_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDV5877069.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNKKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(5762..5995)
                     /gene="hfq"
                     /locus_tag="RJE42_001653"
     CDS             complement(5762..5995)
                     /gene="hfq"
                     /locus_tag="RJE42_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV5877070.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEAQESTESEE"
     gene            complement(6010..6945)
                     /gene="miaA"
                     /locus_tag="RJE42_001654"
     CDS             complement(6010..6945)
                     /gene="miaA"
                     /locus_tag="RJE42_001654"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV5877071.1"
                     /translation="MNKIKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSVVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(6963..7877)
                     /locus_tag="RJE42_001655"
     CDS             complement(6963..7877)
                     /locus_tag="RJE42_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877072.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKDGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISINPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKVLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8027..9700)
                     /locus_tag="RJE42_001656"
     CDS             complement(8027..9700)
                     /locus_tag="RJE42_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDV5877073.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(9858..11354)
                     /gene="glpK"
                     /locus_tag="RJE42_001657"
     CDS             complement(9858..11354)
                     /gene="glpK"
                     /locus_tag="RJE42_001657"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV5877074.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11483..12301)
                     /locus_tag="RJE42_001658"
     CDS             complement(11483..12301)
                     /locus_tag="RJE42_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDV5877075.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVYAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         3819 a         2402 c         1728 g         4409 t
ORIGIN      
        1 aatatcaata gtagtataac taaaatgaaa ataccgatcg ataaacaaaa agatattttt
       61 tgttttgttt ctcttttcat atagtattac ccccttaata atgagtagta aggcccctct
      121 tttcggggtc ttaccttaga acgttctgca aatgaattcg atgagaagta atatgaatgt
      181 ggctattttc aagtaatact caatgttttc gcgacgttct tttatcgcct catctcatca
      241 cctccaaata tattaaaatt catgtgaact aaaatataaa atggtcttcc ccagctttaa
      301 aaaaataaat acataaaaca tttacttgac caaaacttga caaaatccat ttttcaacaa
      361 aaagtggaca gcaattaaaa tttaaagata aaattataga ttgtccacct ttccaaatca
      421 aaaaagagct aaagcaaaac gctctagccc ttgatattac tgatttccca tcattgttta
      481 gcgtattaat attgcttcat gtactgatct ctttcccatt cagatacttg agttctatag
      541 taatcccatt caattgattt tgaattaata aattgattat agatatgatt tcctaaagct
      601 tttttaataa cttcattttc acgcattgct tttaatgcag tataaagtgt tgaaggtaag
      661 tcttgaatgc ctactgcttc acgttcttca cgattcattt cgtaaatatt ttggttaaca
      721 ggttctggaa cttttaattt atttttaata ccgtctaaac cagcttctaa aattgcagct
      781 aacgccatat atgggttcgc agctgggtca actgaacgta cttcaatacg agttgataat
      841 cctcttgaag atggtacacg gattaacggt gaacggtttt taccgctcca tgcaatataa
      901 catggtgctt cataaccagg aactaaacgt ttgtatgagt ttactaatgg gttacataca
      961 gcagtaaatc cgcgtgcatt tttaagcaca cctgctgtaa attgatatgc agtttccgtt
     1021 aagcccattt cagtatttgg atcaaagaat gcattttctt tacctttgaa taatgaaacg
     1081 ttaaagtgca taccgctacc attcacaccg aataatggtt taggcataaa tgttgcgtgt
     1141 aaattatgtt tacgtgcgat tgttttaaca accaatttaa atgtttggat gttatcacat
     1201 gctgtaacag catctgcata tttaaagtca atttcatgtt gaccaggggc aacttcatgg
     1261 tgactagctt caatatcgaa gcccatatcc tctaattcta aaacaatatc acgacgacag
     1321 ttttcaccta aatctgtagg tgctaaatcg aaatatccac catcatcatt aagttctaaa
     1381 gttggttccc ctttttcatc caacttaaac aagaagaatt ctggttcagg ccctaggtta
     1441 aagtctgtga agcctaaatc ttccatttct tttaatacac gttttaagtt tgcacgagga
     1501 tccccttcaa atggtgttcc atctgtttta tatacatcac aaattaaacg tgcaacttta
     1561 ccttgtccag cagtccatgg gaagattacc caagtatcta aatctggatg taagtacata
     1621 tctgattctt cgatacgtac gaaaccttcg atagaagaac cgtcaaacat catttcgtta
     1681 tcaagtactt tttctaattg gcttacaggc acttcaacat ttttaattgt tcctaaaata
     1741 tcagtgaatt gtaatcttaa atatcttaca ttttcctctt ctgcaaattt acgaatgtcg
     1801 tctttagtga aagtacgttt tggcatttta aaatcctcca aattttattt aataaatcgg
     1861 gataaatcac cacgatttat tggcaaagtt tctccaatag gcttttgcgt ggcatctaca
     1921 atcatctttt ttcttatctc ttgttcatct gttgttaaat gctcttgtga gtcataaatg
     1981 atttgtttaa tccctttgat attaaatcct ttttctaata atgatttaat ttctagtaat
     2041 ctttccaaat cattgagtga gaacagacgt ttttgacctt ctgttctttc aggtttgatg
     2101 agttcatgtg tttcatagta acgtatttgc cttggcgtta aatccgttaa cttacttact
     2161 acactcatag agaagacagc catatttcgt ctgattgcat cattcgatat cattgttcct
     2221 ctcctctact tttgaacttg ttaataactt atcataattt ttttaggatt tcaaaaatat
     2281 gtcaggtttt ctaacataaa gctcaaaaca ttgatataac tgcgatttcg acttctaaaa
     2341 cgacataaaa cgaactacag ttaaaatatt ttttcaattt ttaaaaataa tctgataaat
     2401 cttcgcaaaa aaatttcacc caaaacacat tatcatttag ggtgaaattt aatctattat
     2461 ataagtcctt gttctttcaa ctgcttaaca gctcttgtca cagcaatttt aacgtgttca
     2521 tatgttaatc ctccttgaac atatgcttca taaggaggac gaataggtcc atctgcagat
     2581 aattcaatcg atgaaccttg aataaacgta ccagctgcca taataacatc atcttcgtaa
     2641 ccaggcatat aactaggttc tggactaaaa tgtgcattaa ttggggatgc gtgttgaata
     2701 ctttgacaaa atgaaatcat ttgttctttc gttttaaatt taactgtttg aattaaatca
     2761 gttcgttttt cgtagtactt cggcgttgtg ttcatattca ttttttctaa aaataaacta
     2821 gtaaacaatg cacctttaag actctgactg acaacgtgtg gtgctaaaaa gaaaccttga
     2881 tacatttcaa gcaatgcatt taatgatgca cccgcttctt taccaatacc aggtgctgtc
     2941 aatctataac cacatcgttc aattaaatct tttctaccag caatgtatcc accaatctta
     3001 gctaaaccac cgccagggtt tttaattaat gatcctgcta ttaaatcggc accacattct
     3061 ataggttcac gtctttcaac aaattcccca taacagttat ccacaaatat taaaatatta
     3121 ggatgcacgt ttttcaacct agtaattacc tgttcaattt catctagagg aattgacggt
     3181 ctttgatcat agcctttcga acgttgaatc gctattactt tggtgcgctc agaaatccca
     3241 tctaacacac tttcaatatc gattttacct tctttaagtg caatatcttt atatgatacg
     3301 ccgtgctcca ttaaactttc aataccattt ccgtttacac caatgacttc aagtaaagtg
     3361 tcatatggac ttcccgttat ataaattaat tcatcaccat gttttaataa actttgtaat
     3421 gcaatagtaa tcgcatgcgt acctgaaata atttgtgggc gaacaattgc atcttctgct
     3481 ttaaatgcct gcgcatatat ttcttctaaa tgatcacgtc caaaatcatc atacccgtat
     3541 cctgttgatc cttgtagatc actttcggtt gctttgacat gatgaaatgc atttaatact
     3601 ttttcttggt taatatatgc agtttcttca atttctttaa aatatggtgc taacgttgat
     3661 tcgacgtcag ctactatctt acttatatct ttcatttgaa tttcgtcctt ttattatttt
     3721 ttaaaccctt ttatacgata aacattttca atttcatcat atttaagttc agtcactaat
     3781 gtatgttgtt ttagaaaata taatcgatct gcattattcg tcgcaattgc ctcatcataa
     3841 taagttaatt gccttttgat ttcatcaata aataaagtct taacaagtaa tttatcacca
     3901 tcatttttag atgaaacaaa aacattaggc aaatcacttg ctggacgatt tgatgcgtga
     3961 tcacataagt cctttttatt aaaaataact atttgagaaa tatgactcat atctaattgt
     4021 ttgattaaat cattaactgt gtcatactgc gtacggtatt caggatggct actatctacg
     4081 acatgtacta ataaatctgc acctttagcc tcttctaaag ttgatttaaa agctgcaatt
     4141 agcgtcgtag gtagtttctg tataaaacca acagtatctg aaataattaa attaaatcca
     4201 tcatttattt gaatttgtcg tgttttagga tctaacgttg caaataattg atctttttca
     4261 tacgtctctt catttgctaa aacattaaac catgatgatt taccagcatt tgtataacca
     4321 actaaagcta cttgaaacac ctgattttga tttcttttat ttcgatatct ttcgcgatgt
     4381 tcttctaccg ttcgcaattg atgttttatt tcattcatac gagttcgaat atgtctacga
     4441 tccatctcta acttcgtttc accagggcct ctagttccaa taccgccacc taaacgagaa
     4501 agacttttac catggccttg caatctaggt aataaataat caagttgtgc tagctctact
     4561 tgcaatttac cttctttact tcttgctctt aatgcaaata tttcaagaat caactgagtt
     4621 ctatcaataa tttttacacc taaagcttca tttagtgatt tggattgtgc agtcgttaat
     4681 tcatcatttg tgatgactac atcaatatct ttgaactcaa taaatgcttg aatttcttca
     4741 attttacctt taccaacata atatttgcga tctacacgat ctctgttttg agtaatttga
     4801 cccaacactt caagttggca agtctctgat aaagatgata attcctccat tgtagactca
     4861 aaattaaatt gcttatcatc ttgagcatgt acaccgacta atacagcttt ttctagttta
     4921 tttttagtat catgaatttg ttgctgagcc atgttataca tctccttaat aaaatcctac
     4981 tcaaagtatt ctatcataga gttttagtaa agactattac acgattttat ggtacatttt
     5041 aagtaaatcg aaaggatgat tcaatggaga caatttatga ttttgtagta gaaacaaata
     5101 aaggtgttac ttacaaatta gatgcatata aaggtgacgt tatgctaatt gtcaatacag
     5161 ctagcgaatg tggctttact tcacaattcg aaggtttgca atcattatac gagaaataca
     5221 aagatcaagg gtttgtaatt ctaggtttcc cttgtaatca atttggtggc caagaacctg
     5281 gatcaggcga agaagcagct caaaactgta aattaaacta tggtgttacc ttccctatgc
     5341 atcaaaaaat agacgtaaaa ggggaacatc aattaccttt atttagatat ttgactgcgg
     5401 cacaacatgg attctttaat aagaaaatca aatggaattt cactaagttt ttagtggatc
     5461 gtgaaggtaa cgttgttaaa cgatttgcac ctcaaaaaaa acctgttcaa atcgaaagag
     5521 aaatagaaaa gttattataa aatatactag ctcatagcaa ataaaacgct tcatataaag
     5581 tcgtatcata tattgactcg acctttatat gaagcgtttt tattacaaac attcctctat
     5641 tgtatcttgt catttgagca tcatttaatt gatgtcacat atgactttat ttctatatct
     5701 atttatcata cttcaaacgt tatgtacacg agtcattaaa tcgtgcccat atgattacaa
     5761 cttattcttc actttcagta gattcttgag cttcagtttc tactgtataa gtgctgatcg
     5821 catgtttgta aatcaagtgt tgtttgcctt gagaatttaa gcttacgaca tacttgtcgt
     5881 attcttcaat aacacctttc atttggaaac cgtttagaaa gaatacagtt acttcagttt
     5941 ggtttgcttt aaaattctct agtgctttgt cttggatgtt ttcgtttgca atcatctgtc
     6001 ggactccttt tacttaatct gggttgtaat ctcatctaac atcatttgaa gtgacatatt
     6061 ttctttatct aaccaatgaa cactcatttt attcttgaac catgtcattt gtcgttttgc
     6121 atattggcgt gaatgttgct ttaaatcatt gacagcatct tcataaatca tttgtccgtt
     6181 aatcacaggt attaattctt tatatccaat agcttgcata ctttggcaag attcatagcc
     6241 ttgttcaaca agttgttgca cttctctaaa taatccgtga tccaacataa tatcaacacg
     6301 tttatttatt cttgaatata atgttttacg cgacatttca atccctaata ataatgtatc
     6361 ataattttca gtaaattgtt gcactttctt gcgattactc aaaagttttt ttgtttttaa
     6421 ataatattca atagcgcgca acactctttg gcggttgtta gggtgaatat tttctgcaga
     6481 aaccgcatca aattgagcta aataatcgtg tagttgctga ttatctaaat gttctaatgc
     6541 agataacttt tgtttaacta cggataattg tgcaggtgta actgtttcat cttctaattc
     6601 ataattatat attaatgatt gaatatataa gcctgttcca cctgctatga ttggaacttt
     6661 acctctattc gttatatcag taattaaatc ttctgctaat cgcttgaatt catatgctga
     6721 aaatgtatca tcaggattca agatatcaat taaatgatgt ggaataccat ccatttcttc
     6781 aggtgttact tttgcagttc caatattcat atgcttgtag acttgcatag agtcaccgct
     6841 tatgatttca ccattgatac gcttcgcgag ttcgatgcta agctctgttt tacctgaagc
     6901 agttggcccc acaattacta caataaaagg cttaatttta ttcatattta attccacttt
     6961 ctttattttt tataaaattg attcaaccat ttaaacaagt cttcccaaat tttgtcatga
     7021 tctttttcaa ataacacttc atgacgttta tgatgataaa gattaacttt aacattttta
     7081 atgccggctt ttctatatat attcgccaat tttaaaatcc ctttaccata atcacctaaa
     7141 ggatcgtcat atcctgaaac taataatata ggcatggcat gatttaatac ttttatattt
     7201 tttaattgtg atgtatgtag catataatag gctgtttgat ataataattg atttgacaca
     7261 ttaaatccac tatatggatc tttaataaag ttgtctactt cagttggatt aatagaaatc
     7321 caatcacttt gtgtacgtaa tggacgtatt tttttattaa aactatttga tactaactgg
     7381 ttaacccatt caacccgttt ctcagcacca taaatttttg taatcagttg taacacctta
     7441 acggttggta atcctttcca taatggatac ataccagtac cacttaaaat tagaccatca
     7501 acatattgcg gatatgtttc tacaaacaat ctagctataa cggatcccat tgaatgtccg
     7561 attataatgt atggtttatc aacattgcct ctcactgttt gcgctacttc aaaggcatca
     7621 ccgataactc gtttcatatc atcgtaatgc cctcttgttg attcatcaat attaatacca
     7681 tgtcctcgat gattatgacg tatcacatcg aagccatcct ttgaaagtgc atgtgctaat
     7741 ttatcgtaac gttccatatg ttcagccata ccatgaaata tatgaataat tccttttgaa
     7801 tctacatctg taaaattcac tttaacttca atattcgtat tatcttgtac agttaattta
     7861 aattgttttt cagtcatcat ttcacacctt taataacaac gtttttatat tcgcttttat
     7921 tattatcata catttagtga tgcctagttt caattgacta ttgtatctca tataaaaaaa
     7981 ctgatgcccc taaaacagga caccagttta tgattgtaca aattaattat tctttaactg
     8041 ctggttgatt atttccatgt tgcgcttctt taattgcttg ctcaacttct tcagtataag
     8101 cctcaatttg agctggtgag tagtctaaca tatcagccat aatatcgatg atactatctt
     8161 tataatctaa tacatcttta atattgaagt acatttttcc agaacgacga actaagaaat
     8221 cgttaggttt gtataccatt tcttgttgaa tactataaac aagttctaca taaatttcta
     8281 atggtaactt gctatcatgg tattgagatg tttgcgcaat gttgaacaat tcatcaacat
     8341 ttgaaccata tttagatgct aaacgtcttg caacatcttc atcaatgcca aatcctttag
     8401 ctacatctac tttttgctct acaaacgcat caaagttctt gctaccacct acatcgccac
     8461 cagaaattgc cagacctttt gtattacatg gactaaatgt taaaccatag tcttttttca
     8521 agcgtttaga tactaaatca acaatgtctt gagccatgtg acgatagcct gttaatttac
     8581 cacctgcaat agttaataaa cctgatttac cttcccaaat ttcatcctta cgagagattt
     8641 cagaagggtc tttgccttct tcgtaaatta atggtctaat tcctgcccat gttgattcaa
     8701 tatcttcatc tgtaacatta acactaggga acatgtaatt aatcgcatcg attaaatagt
     8761 ctctgtcttc ttgtgtagtt aatggtgaag atttgatgtt gtcatagaat gtatctgtag
     8821 tacctacata cgcttttcct tcacgcggga ttgcaaaaat cattcttcca tctttttcag
     8881 tatcaaagta tactgcttga cctaatggaa attttgattg atcaataaca acatgtacac
     8941 ctttagttaa acgtaatttt ttattattgc gtgcataatc accacttcta acatcatcaa
     9001 cccatggacc tgctgcatta accacttttt tagccttaat tgtataattt tcattagtta
     9061 atttatctat aactttaaca ccatttactt gttgattttt atcataagtg aagtgttcag
     9121 acttagtata gttgataatt tctgcgcctt tttcagcagc acgcttcata acttcaatag
     9181 ttaaacgcgc atcgtcagta cgatattcaa catagtaacc gccgcctttt agaccttctt
     9241 ttttaactaa tggttcttta gctaaagttt cttttttaga taacattttt ttacgttcag
     9301 actttttaac acctgctaaa cgatcataca ttcctaaacc aattgatgtt gagaatttac
     9361 caaatgttcc acctttatgc attggtaaaa gcatccactc tggagtcgta acatgaggcc
     9421 cattttcata aacaatcgca cgttctttac cagtttcggc aactactcca atttggaatt
     9481 gttttaagta acgtaaacca ccatggacta attttgtaga tcttgaactt gttccttgtg
     9541 caaagtcttg catttcaact aatgcaactt tcattcctct ttcgctcgca tctagtgcaa
     9601 tacctgcacc tgtaataccg ccaccaataa ttactaaatc atattcatca tttcttaaat
     9661 tctttttaat atgttctctc ttaaaagtag acaatgccat tccaaaacgc ctcctaaaaa
     9721 atgtaaaaaa gagagacttg tcccacataa acaatccttc aattgtacat gttcgaacaa
     9781 atctctcgtt tctcgattca aatattaact taaattgatt gtatcacaat gtttatacaa
     9841 aagtctaatc tacaagttta ttctgtttta aaaacttgtg ttgcttcaac agcttttttc
     9901 caacctctat ataatttttc tctttcgcct tcatccattt tcggatcgaa tttttcttct
     9961 aatttccaat ttttagcgat atcatcttta ctctcccaga atccaactgc taaacctgct
    10021 aaatatgcag cacctaaagc tgtagtttct tgaatttcag gtctttcaac agaagtatta
    10081 acaatgtctg cttggaactg cataataaag ttattcttaa ctgcaccacc atcgacacgt
    10141 aaactttgga catcgatacc agagtctttt gacattgctt ccataacgtc acgagtttgg
    10201 taacataatg attctaatgt tgcacggata aagtgctctt tttcagttcc gcgtgttaaa
    10261 ccgaaaatcg caccacgtgc ttcagaatcc caatatggtg ttcctaaacc tacaaaagct
    10321 ggaacaacat aaacaccctc agtagagtca actcgtgtcg cataactttc tgattgtggt
    10381 gctgaattaa tcattcttaa tccatcacgt aaccattgga ttgctgaacc cgaaacaaag
    10441 attgaacctt caagcgcata atttactttt ccatcaatac cataagcaat tgttgttaat
    10501 aaaccacttt cagatttaac cgctttgtca cctgtattca ttaacatgaa gccaccagtt
    10561 ccatatgtgt ttttcacatc accacgttcg aagcaagctt gtccaaataa tgctgcttgt
    10621 tgatcaccag ctactccagc gattggtact tcttgaccat agaagtggta atcaattgtc
    10681 ttaccatata cttcactcga agctttaact tctggcaaca tatttttagg tactgtaagt
    10741 agttctaata actcatcgtc ccattctaaa tcatggatat taaacattaa tgtacgactc
    10801 gcatttgaat aatcagtaat atgcgcagct tttcctgata atttccatac taaccaagta
    10861 tcaatcgttc caaataatag atcgccattt tctgcttttt ctcgtgcacc ttcaacattg
    10921 tctagaatcc atttaacttt tgtacctgca aaatacggat ctaaaagtaa tcctgtctta
    10981 tctctaaatg tttgttcata tccttgttgt tttaattctg aacaaattga ttgtgtctga
    11041 cgtgattgcc aaacaattgc gtgataaatt gggcgaccag tatgtttgtc ccaaacaacc
    11101 gttgtttcac gttggtttgt aataccgata cctgcaattt gatcagctct aacatcattt
    11161 tcattaatta cttccgtcat tacagctaac acagatgtcc aaatttcatt tgcatcatgt
    11221 tcaacccaac ctgattgtgg aaaatattgc ttaaactcac gttgtgctac ccctgcaatt
    11281 tccccttttt gattgaataa aatcgctctt gagcttgttg ttccttggtc tatagataaa
    11341 atatattttt ccattagtag tatctccttt attaaaaaat aatcgctaac ccattgtaca
    11401 aaatctgtta aattttggcg aaaaaatagc ataatccatg aatcaaacat gttaaattat
    11461 gctatttacg tttcttttta ttttagtaaa ttgattcgat atcacctttt tttgatgatt
    11521 tatttaaaat gtaacctaaa atcaaagtaa taataactac aacaattgca attgcacaac
    11581 caatattaaa tgtatgttta taaaatacag cgtatactac cgcacctaat aaaccaccgg
    11641 caattggtcc taagatagga acgattgcat atgaccaatt tgaaccacct ttaccagcta
    11701 ttggtaaaat cgcatgtgca attctcggac ctaaatcacg tgctgggttg attgcataac
    11761 cagtagcacc gcctaaactt aatccgattg caacaattaa tgctccgaca attaaaggat
    11821 ttaaaccatc ggcaattttg tttacaccga taaataaaat acctaaagtt aatgccattg
    11881 ttccgataat ctcacttaaa aagttggcaa agtaattctt aatagctggt gctgtagaga
    11941 aaacacctaa tttcgcgcca gcttcttctg tcgctttcca atgtggcaag tacattaacc
    12001 atacaattgt tgctccgaca attgcaccta acatttgagc aacaatataa ccaggaacta
    12061 atgaccaatc aaaacttccg tctaatgcaa gagctaaaga caccgctggg tttaaatgtg
    12121 cacctgagaa ttgaccgaca gcatacacac ccattgtaac cgctaatccc catccagctg
    12181 tgatgacaat ccaatcagca ccattcgcag cacttctctt taaattgaca ttggcacaaa
    12241 cgccaccacc aaaaaggatt aagattgcag ttcctaggaa ttctgctaaa tatacattca
    12301 tattaaatgt cctcccaaaa agacaaagag attcctacac tagcagtttg gctcgtag
//
LOCUS       DAPIDM010000070        12323 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000070 DAPIDM010000000
VERSION     DAPIDM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.070"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..243
                     /locus_tag="RJE42_001659"
     CDS             <1..243
                     /locus_tag="RJE42_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5877076.1"
                     /translation="KIYSHRKIYVEPVFGFMKVILGFTRMSVRGINKVKRELGFVLMA
                     LNIRKIAAQRAVHYKIHIKKANFHQIINRNQLFYIA"
     gene            complement(379..1281)
                     /gene="cvfB"
                     /locus_tag="RJE42_001660"
     CDS             complement(379..1281)
                     /gene="cvfB"
                     /locus_tag="RJE42_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDV5877077.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            1428..3029
                     /locus_tag="RJE42_001661"
     CDS             1428..3029
                     /locus_tag="RJE42_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV5877078.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIDDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3888..5093
                     /locus_tag="RJE42_001662"
     CDS             3888..5093
                     /locus_tag="RJE42_001662"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDV5877079.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHLTISYPLSDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            5157..6146
                     /locus_tag="RJE42_001663"
     CDS             5157..6146
                     /locus_tag="RJE42_001663"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV5877080.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGI
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            6148..7035
                     /locus_tag="RJE42_001664"
     CDS             6148..7035
                     /locus_tag="RJE42_001664"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV5877081.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAKAIIVN
                     GTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQSSIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPSIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALFDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            7032..7754
                     /locus_tag="RJE42_001665"
     CDS             7032..7754
                     /locus_tag="RJE42_001665"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV5877082.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTSKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEAFHLPLVVATTGEKEKLLNKLDELSKNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7781..8500
                     /gene="dapD"
                     /locus_tag="RJE42_001666"
     CDS             7781..8500
                     /gene="dapD"
                     /locus_tag="RJE42_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDV5877083.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8643..9794
                     /locus_tag="RJE42_001667"
     CDS             8643..9794
                     /locus_tag="RJE42_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV5877084.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVVAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMQKIAESVKLL
                     FNVDCEIKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKMLINVANFYENLLNNYKEV"
     gene            9799..10884
                     /locus_tag="RJE42_001668"
     CDS             9799..10884
                     /locus_tag="RJE42_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV5877085.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLSGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVESLLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKANVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10874..12139
                     /gene="lysA"
                     /locus_tag="RJE42_001669"
     CDS             10874..12139
                     /gene="lysA"
                     /locus_tag="RJE42_001669"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV5877086.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCLQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAIKAINKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12233..>12323)
                     /locus_tag="RJE42_001670"
     CDS             complement(12233..>12323)
                     /locus_tag="RJE42_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HDV5877087.1"
                     /translation="NNIIEKLEDESNASSVAVAEVMKNIHPYS"
BASE COUNT         4270 a         1929 c         2267 g         3857 t
ORIGIN      
        1 aaaatctata gtcatagaaa aatttatgta gagcctgttt ttggatttat gaaggttatt
       61 ttgggtttca ctcgaatgtc agttcgagga ataaataaag ttaaacgaga gctaggtttt
      121 gtattaatgg cacttaatat aaggaaaata gcagctcaac gagctgtaca ttataaaata
      181 catatcaaaa aagctaattt ccatcaaata attaatagaa atcagctttt ttacattgcc
      241 taagaactta atgtcccagc ctattcgttt gaattattta attttttact ttgaaaggaa
      301 tgtttaaaaa actacttcat ctgtccgatt taatattgaa tataaaaaac acgcatccta
      361 cgatacgtgt aaaaatgatt attcttttga gtcaattcga ctccaacctt ttttagttaa
      421 agtgatttta ccagtttcta tattaataat cttctgttta tataagtgac cgattgcacg
      481 tttgaatgaa cctttactca tattgaatac ttctttaatc gcttcagggc ttgatttgtc
      541 ccagaatggt aattcaccat catattcaac tagtaaatca aagatgactt gaccgtcatc
      601 gtctaaacgt tcatgtgcaa gtggtaaaaa tgaaccattt aactcacctt tatcattatg
      661 cccgataatt ctaacttgaa cagattcacc tagtctcggt tcagctttac gttctgattc
      721 atgtacgaat attttgtaac ctgattcgct taataagaag ctaccaattc gtaatacgcg
      781 gtaaggtttg gcttcaatga cttcgttttt taaattatcg tcgtgtacag gtgtaaacat
      841 attttctaca acagattcac tcgctaaacg tccatacata tgattctcac ggtcaattcg
      901 taatgtaact agcaaatgat cacctggttg tggccatagt gatttcactt ttggtaaatc
      961 ttcccatggt actaacactt cacgaggtaa tccaacatct atacgtgccc catcgcgatc
     1021 cgttttaagt actttagcaa aatcatattt atctttcgta atatcaggca tattttgagt
     1081 tgcaaataat tcacctgaac ggtttggata aatgaagaaa ctatattctt cacctacttc
     1141 taattcatca tcatcgttca tttctgattg gtttaacttt acgttttcac cgtttggtcc
     1201 ttttaaaagg taagttgagc cttgtaaccc tactacttca aggaattcta tagaacctac
     1261 tatatctttg tctaatgcca tgttattctc cttcgattct tgtattcttt attattataa
     1321 catgtgtgag tcattctatc attacaactt atatgaggtc gaaactgtga cgataatatg
     1381 atataattct tccgttagaa tatcgaatga aaaaaaggag aaaatgcatg ttacaagtaa
     1441 ctgatgtgag tttacgtttt ggagatcgta aactatttga agatgtaaat attaaattta
     1501 cagaaggtaa ttgttatgga ttaattggtg cgaatggtgc aggtaaatca acattcttaa
     1561 aaatattatc tggtgaatta gattctcaaa caggacatgt ttcattaggt aaaaatgaac
     1621 gtctagctgt tttaaaacag gaccactatg cttatgaaga tgaacgcgtg cttgatgttg
     1681 taattaaagg tcacgaacgt ctttatgagg ttatgaaaga aaaagatgaa atctatatga
     1741 agccagattt cagtgatgaa gatggtatcc gtgctgctga acttgaaggt gaatttgcag
     1801 aaatgaatgg ttggaatgct gaagctgatg cagctaacct tttatccggt ttaggtatcg
     1861 atccaacttt acacgataaa aaaatggctg aattagaaaa caaccaaaaa attaaagtat
     1921 tattggcaca aagtttattc ggtgaaccag acgtactatt actggatgag cctactaacg
     1981 gtctggatat tccagcaatc agttggttag aagatttctt aattaacttt gataatactg
     2041 ttatcgtagt atcacatgac cgtcatttct taaataatgt atgtactcat atcgctgatt
     2101 tagactttgg taaaattaaa gtttatgttg gtaactatga tttttggtat caatctagtc
     2161 agttagcaca aaagatggct caagaacaaa acaagaaaaa agaagaaaaa atgaaagagt
     2221 tacaggactt tattgctcgt ttctcagcta acgcttctaa atctaaacaa gcaacaagtc
     2281 gtaaaaaaca acttgagaaa attgaattag atgatattca accatcatca agaagatatc
     2341 ctttcgttaa attcacgcct gagcgtgaga ttggtaacga cttattaatc gttcaaaatc
     2401 tttctaaaac aattgatggc gaaaaagtat tagataatgt atcattcaca atgaatccaa
     2461 atgataaagc gattttaatt gatgatagtg aaattgcaaa aacaacatta cttaaaatat
     2521 tagctggcga aatggaacca gacgaaggtt catttaaatg gggtgtgact acatcattaa
     2581 gttacttccc taaagataac tcagagttct ttgagggtgt aaatatgaat ctcgttgatt
     2641 ggttaagaca atatgctcct gaagatgaac aaacagaaac atttttacgt ggtttcttag
     2701 gtcgtatgtt atttagtggt gaagaagtta agaaaaaagc tagtgtgctt tcaggtggag
     2761 aaaaagtacg ttgtatgcta agtaaaatga tgttatcaag tgcgaatgta cttttacttg
     2821 acgaacctac taaccactta gacttagaaa gtattactgc tgtcaatgat ggtcttaaat
     2881 catttaaagg ttctatcatc tttacttctt atgacttcga atttatcaac acgattgcaa
     2941 accgtgttat cgatttaaat aaacaaggcg gcgtttcaaa agaaattcca tatgaagaat
     3001 acttgcaaga aatcggcgtt ttaaaataaa cataatatgt agaatttctt ttcgaaaatc
     3061 tctgtgttga ggcccggcca acttgcacat ttttgtaagt tgacttactg taaccttcta
     3121 tattgggact tattccccaa ctcgtattgt ttgtagaaat taggaatcca atttctctat
     3181 gcgtggcgcc gacaaggatt gaaaaaagct agctacaaag tcattttcat tcagtcaact
     3241 actgtcaata taacattgca gggcctaggg cattgattca tgtccgagac tatattcaat
     3301 ttaatagaca ttaactgggc gtctcgatgc taatatttat agcatcgagg tgtccttttt
     3361 attttatttt ttaacacatc agcacagtga acaccccttc tataaattac aaattcagtt
     3421 atcaacgcaa taaaagaata catttattta atgtgcatat tgagtatgct ataagtattt
     3481 tggattttat ttttcaaaaa gtctcattat cacacctttt ataatataag caacgctata
     3541 acttgaattt tcaggttatt ccaaaaaatc taaattatcg gtttacattc atatttgtca
     3601 tatgtataat gaacacatac caatttaata atattttgat gaggcgcatc aatcatgagt
     3661 aaagtttaga ttactgtctg ctaacagcta aatttgaaag ggtgcgatgc cgaagcgatt
     3721 ataatagcag ttataatttg ttggactttt tggttaagag ctgagagttt gtcattattt
     3781 aaaaataatg gagtgcatca cttgtatata gattaagaac aagttcgcat tccgaacttg
     3841 ttcttttttt attattgtgt gcccttccta acaattagga ggattatatg gtaacaagaa
     3901 gtgtgttgaa atttggcgga tcatccgtca gtgattttac aaaaataaaa aggatcgctg
     3961 aaatgttaaa ggagcgagtc aatcaagatg aacaattaat tgtcgttgta agtgctatgg
     4021 gtaacacaac agatcaatta atgacgaatg tatcgacctt gactaaagca ccaaaacaac
     4081 aagaactggc attattattg acaaccggag agcaacaaac tgtatcttat ttatcaatgg
     4141 tattaaatga tatcggtgtg aatgccaaag caatgactgg ctatcaagcg ggtattaaaa
     4201 ccattggcca tcatttaaaa agtaaaattg ctcaaattaa tcctcaaaca tttgaacaag
     4261 cctttcaaga aaacgatatt ttagtagttg ctggatttca aggcatcaat gaacatcagg
     4321 aattaacaac tttaggcaga ggcggttctg atacgaccgc tgtggcactt gctgttagta
     4381 atcaaatacc ttgtgaaatt tataccgacg ttgatggtgt gtatgccact gacccaagac
     4441 ttttaccaaa ggctaaacga ctagacatcg tctcatatga agaaatgatg gaaatgagcg
     4501 ctttaggtgc tggtgtactt gaaacaagaa gtgttgaatt agctaaaaac tataatatcc
     4561 ctttatattt aggaaaaact ttatcgaacg tgaaaggaac atggattatg tcaaatgaag
     4621 aaatattaga gaaaaaagca gttactggtg tggctttgga taaacatatg atgcatctaa
     4681 caattagtta tcccctatct gacaatcagc tacttaccca actatttacg gaacttgaag
     4741 aaggtgctgt aaatgttgat atgatttcac aaatcgtcaa cttggatggg ctacaactat
     4801 ccttcacgat taaagatagt gattttcatc aaatttctat gattcttgaa acattaaaga
     4861 atcaatatga agcattagct tataaaatca atgagcatta tgtcaaaatt tcattaattg
     4921 gctcaggcat gcgtgatatg tcaggtgtgg catcaaaagc atttttgaca ttaattgaaa
     4981 ataatatacc tttctaccaa acaacaacat ctgaaataag tatttcatac gtcattgatg
     5041 attttaatgg gcaacaagcg gtagaaaaac tatatgacgc atttaacatt taatggtaaa
     5101 atgattgtta aaatattcta aaaattggaa attattataa aatggagtga caagttatga
     5161 caaagttagc agttgtgggt gcaacaggat tagtaggcac aaaaatgttg gagacattaa
     5221 atcgtaaaaa tattcctttc gatgaattag tattattttc atcagcacgt tctgcagggc
     5281 aagaagttga atttcaagaa aaaacatata cagtacaaga attaactgat gctcgtgcaa
     5341 gtgaacattt cgattatgta ttaatgagtg ctggtggcgg tacaagcgaa cactttgccc
     5401 cactctttga aaaagctggt gcaatcgtta tagacaattc aagtcaatgg cgtatggcag
     5461 aagatattga tttaatcgtt ccggaagtca atgaacctac atttacaaga ggtatcattg
     5521 ccaatccaaa ctgctctacg attcaatctg ttgtgccatt aaaagtattg caagatgctt
     5581 atggtttaaa acgagtggca tatacaacat atcaagctgt atcaggttca gggattaaag
     5641 gtaagaaaga tttagctgaa ggtgtaaatg gtaaagcacc agaagcatat ccacatccaa
     5701 tttataataa tgtgttaccg catattgatg tgtttttaga aaacggatat acaaaagaag
     5761 aacaaaaaat gattgatgag acgagaaaaa ttttaaatgc gccagactta aaagtaacag
     5821 caacatgcgc acgtgtgcct gttcaagata gtcatagtgt tgaaattgat gtaacgcttg
     5881 acaaagaaac gacagcagaa gatattaaag cgttatttga tcaagatgac cgcgttgttt
     5941 tagtagacaa tccagagaac aatgaatatc caatggcaat caattccact aataaagatg
     6001 aagtatttgt tggccgtata cgtagagatg attcattaga aaatactttc catgtatggt
     6061 gtacatcaga caatttatta aaaggtgctg cattaaatgc tgtacaagta ttggaacaag
     6121 ttatgcgttt aaaaggagcg aattaaaatg acacatttat ttgagggtgt tggcgttgca
     6181 cttacaaccc cttttacaaa taacaaagtt aatcttgaag ctttgaaagc acacgttaat
     6241 tttttactag aaaataatgc caaagcaatc atcgtaaatg gaactactgc tgagagccct
     6301 actttaacaa cagatgaaaa agaactcatt ctaaaaacag ttattgatct tgtagataaa
     6361 cgtgttcctg tcatagcagg aactggaact aatgatactg aaaagtcaat ccaatcttca
     6421 atccaagcta aagccttagg ggctgatgca attatgttaa ttacgcccta ctacaacaaa
     6481 acgaatcaac gtggtttagt aaaacatttt gaagcgattg cagatgctgt gaaattacca
     6541 gtcgtgctat acaatgtgcc atcaagaaca aatatgacta ttgaaccaga aactgtagaa
     6601 atattaagtc aacatccttc tattgtagct ttaaaagatg ctacgaatga ttttgagtat
     6661 ttagaagaag tgaaaaagcg aattgataca aattcatttg cattatatag tggcaatgat
     6721 gacaacgtcg tcgaatacta tcaacgtggc ggtcaagggg ttatctctgt tattgccaat
     6781 gtcattccta aagaatttca agcgttattc gatgctcaac aaagtggatt agatattcaa
     6841 gatcaattta aaccaatcgg cacactgtta tcagccctat cagttgatat taacccaatt
     6901 cctattaaag ctttaacaag ttatttagga tttggaaact atgaattacg tttaccattg
     6961 gttagcctag aagatacaga tactaaagtg cttcgtgaag catatgacac atttaaagcg
     7021 ggtgaaaatg agtgaaaata ttactaattg gctatggtgc aatgaatcaa cgcgttgcta
     7081 gattagcaga agaaaaagga catgaaatcg ttggggtcat tgaaaataca tcgaaagcaa
     7141 caacgccata tcaacaatac caacatattg ctgatgttaa agatgccgat gttgcaatag
     7201 atttttcaaa tccaaatctg cttttccctt tattagatga agcgtttcat ttgccattag
     7261 tcgtggcaac aactggtgag aaagaaaaac tacttaataa gttagatgaa ttgagtaaaa
     7321 atatgcctgt atttttcagc gcgaacatga gttatggcgt tcatgcactg actaaaattt
     7381 tagcagctgc tgttccccta cttgatgatt tcgacatcga attgactgag gcacatcata
     7441 ataaaaaagt agatgcacca agtggtacgt tagaaaaatt atatgacgtg attgtatctt
     7501 tgaaagaaaa tgtaactcct gtttatgata gacatgaatt aaatgaaaaa cgccagccac
     7561 aagatattgg tattcattct attcgtggag gtacgattgt cggtgaacat gaagttctat
     7621 ttgccggcac tgatgaaacg attcaaatca cgcatcgtgc acaatcaaaa gatatttttg
     7681 cgaatggtgc aatacaagca gcagaacgct tagttaataa accaaacggc ttttatacgt
     7741 ttgacaacct ataaacatat taaaggagat cgattatttt atggtacaac atttaacagc
     7801 tgaagaaatt attcaatata taagtgatgc taaaaagtct acaccaatta aagtatattt
     7861 aaatggcaat tttgaaggca tcacatatcc agaaagtttt aaagtatttg gttcagaaca
     7921 atctaaagta atcttttgtg aagcagatga ttggaaacct ttttacgaag catatggtag
     7981 tcaattcgaa gatatagaaa ttgaaatgga tcgtcgcaat tctgccattc cattaaaaga
     8041 cttaacaaat actaatgcac gtattgaacc aggtgcattt attagagaac aagctattat
     8101 tgaagatggt gctgtcgtta tgatgggtgc aacaattaat attggcgcag tcgttggcga
     8161 aggtacgatg attgatatga atgctactct cggtggtcgt gctacaactg gtaaaaatgt
     8221 acatgtaggg gctggcgcag tattagcagg tgtgattgaa ccccctagtg cttcaccagt
     8281 tataatcgag gatgatgtat taatcggtgc aaatgcagtt attttagaag gtgtacgtgt
     8341 cggtaaaggt gctattgttg cagctggcgc gattgtgaca caagatgtac cagctggtgc
     8401 agttgttgct ggtacacctg ctaaagtgat taagcaagct tctgaagtac aagatactaa
     8461 aaaagagatt gtagcagcat taagaaaact aaatgactag tgacgtcaac gtataataat
     8521 ttcaaggttg agatacgatt atgtctcaac cttattgttt atcattaaat aaacttatga
     8581 tcacaagttt cgaaatacgt tacactgttt atatagtaat ttaacacaaa ggattgataa
     8641 ttatgaatga attagaattt gttacgaaac atcgccgtca tttacatcaa catcctgaat
     8701 taagcttaca tgaatttgaa acaactgctt atattaaagc gtttttagat agtttaaata
     8761 ttaaatacga ttgcccatta gaaactggcg tcgttgcata cttagaaggt aacggctcac
     8821 atacgatagc gtatagagct gatattgatg cgttacctat tttagaggaa aatgatgtac
     8881 cttatcgcag tcaatctgat catgtgatgc atgcttgtgg acatgatggt catacaactg
     8941 cattaatgct tttcgtacaa cgttgcaaag acatgcaaga tgcggggcaa ttaccgcaaa
     9001 atgtcgtttt cattttccaa cctgcagaag aaactggtgg cggtgcaaat cgattaataa
     9061 aagccggtgc ctttgataag tatccaattg aagcggtatt tggtattcac gttaacccat
     9121 ttgctgatga aggcattgca gtgataagag atgaagaaat tacggccagc gcaacagagt
     9181 atcgcttttt cttaacaggc ctgtcaagtc atgttgctga taaagaacaa ggtcattctt
     9241 gtggtgaagc attacaacat gtattaactc aaatatcaca aattcaacaa tttcacctta
     9301 acggtttgaa acgaaatatt gttcatattg gtcattttaa agctggtgaa gcgattaaca
     9361 ctgtaccaag taatggttat ttagaaggta caattcgtac atatgatatt gatgatttaa
     9421 caatcgttaa aaatcaaatg caaaagatag cagaaagtgt caagcttctg tttaatgtag
     9481 actgtgaaat taaatttgca gaaggttatc cccctacaat caatagtccg aaattacgta
     9541 ctcaaataga ggacgcctta ataaaagctg atttaaatgt ctatgacaaa ccaacgccat
     9601 tcttatttgg ggaagatttt agtttttatg gtcaacaact ggctcctgct tactttgttt
     9661 ttataggaac acgaaatgaa gataaaggtt ttgtaactgg tttgcacaca tcgcatttaa
     9721 attttgatga aaaaatgtta ataaacgtgg ctaattttta cgaaaattta ttaaataatt
     9781 acaaagaggt gtaatacatt gacagcaaca tggtctgtaa ataagaaaat atttttacaa
     9841 aatgcaatca cagtcaaaaa caatcagcca ttaatggcag ttgttaaaaa taatgcatat
     9901 cactatgacc tagaatttgc tgtaactcag tttatccatg caggtataga tacatttagt
     9961 acaacatcac tacgagaagc aattcaaatt agacaacttg ctccagatgc aacaatcttt
    10021 ttaatgaatg cagtttacga gtttgattta gtccgtgaac atcaaataca catgactttg
    10081 ccgtcgttga catattacta taaccataaa aatgatttat caggtattca tgttcactta
    10141 gaatttgaaa atttattaca tcggtctgga tttaaagatt taaacgaaat taaagaagta
    10201 ttgaaagatc accatcataa tcaaaatgca aaaatgatta ttagtggttt atggacccat
    10261 tttggatatg ctgatgaatt cgatgtgtca gattataatg ttgaacgttc acaatggatg
    10321 gaaattgttg aatcactttt atctgaaggt tatcagttcg acttaatcca tgctcaaaat
    10381 agtgcgagtt tttatcggga aggacaaata ttactacccc accatacaca tgcgcgtgta
    10441 ggtattgcgt tatacggttc aagaccatat agttcactga atcaacatga tattgttcag
    10501 tcattgactg taaaagcaaa tgttattcaa gtgcgcgagg tacaagctgg tgattattgc
    10561 ggttatagct ttgcctttga agtgactaaa aacaatacaa aattagctgt agttgatatc
    10621 ggttatggcg atggaatttt aagaactcgt gctaaacatg aagcacttat caatggtaaa
    10681 cgctacccga tacgtgcatt aatgatgagc catatgtttg ttgaagtaga tggcaatgta
    10741 catgcacaag atgaagttat tctttataat aatgatatcc gcatcgatga atataccttt
    10801 aaaggtgttg gtgcaaattc tgaacaatta agtgctatga atcatgattc tttaaaaaag
    10861 gagtacattt caaatgactg ttaaatataa tcaaaatggc gaattaacaa tggatggtat
    10921 tagtttaaaa acaattgcgc aaagctttgg tacacctacc attgtttatg atgaactaca
    10981 aattagagaa cagatgcgcc gttaccatcg cgcatttaaa gatagcggtt taaaatataa
    11041 tatttcatat gcctctaaag catttacttg tcttcaaatg gtcaaactgg tagcagaaga
    11101 agatctacaa cttgatgttg tttctgaagg tgaattatat acagctttag aagcaggttt
    11161 tgaaccgagt cggatccatt tccatggtaa caataaaacg aaacatgaaa ttaggtatgc
    11221 tttagaaaat aacatcggtt attttgttat agattcatta gaagaaattg aactaataga
    11281 ccgctatgct aatgatacgg ttcaagttgt attacgagtt aatccaggtg ttgaagcaca
    11341 tacgcacgaa tttattcaaa ctggtcaaga agatagtaag tttggattat caattcaata
    11401 tggcttagct ataaaagcaa ttaacaaagt gcaacaatct aaacacttaa aattaaaagg
    11461 tgtacattgt catattggtt cacagattga aggtacagaa gcatttattg aaactgctaa
    11521 aattgtttta cgttggctta aagagcaagg cattcaagtt gaattattaa accttggtgg
    11581 tggctttggt attaaatatg ttgaaggtga tgaaagtttc cctatcgaaa gtggtattaa
    11641 agatattaca gacgcaataa aatccgaaat taaagttcta ggcatagatg caccagaaat
    11701 aggtattgaa ccgggacgat ctattgtagg tgaagctggc gttactttat atgaagttgg
    11761 aaccattaaa gaaattccag agattaataa atatgtttca atcgatggcg gtatgagtga
    11821 tcatatcaga actgcacttt atgacgcaaa gtatcaagca ttgctagtta atagaaatga
    11881 agaagcagat gacagtgtaa ctatagctgg aaaattatgt gagtctggtg atatcattat
    11941 taaagacgct aaattacctt catcagtcaa acgcggagac tatcttgcta tattatcaac
    12001 tggtgcatat cattactcta tggcatccaa ttacaaccaa atgcaaaagc cttctgtgtt
    12061 tttcttaaaa gatggcaaag cacgtgaagt tataaagcga caatcgttaa gacaactcat
    12121 tattaatgat acaaaataaa aataaacaaa gtaatccccg aagcacagca aattatactg
    12181 tgtctcgggg aatttttaag attatttaat taagagttta ttgcttgttg gtttatgaat
    12241 atggatgaat atttttcatt acttcagcta cagcaacact acttgcatta gattcatctt
    12301 caagcttttc aattatgtta tta
//
LOCUS       DAPIDM010000071        12291 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000071 DAPIDM010000000
VERSION     DAPIDM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.071"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(10..1110)
                     /locus_tag="RJE42_001671"
     CDS             complement(10..1110)
                     /locus_tag="RJE42_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDV5877088.1"
                     /translation="MKIADLMTLLDYHVPFRTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGSIIRKLIQHDINLIAMHTNLD
                     VNPHGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDACQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADFKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIQGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFQYI"
     gene            complement(1113..1790)
                     /locus_tag="RJE42_001672"
     CDS             complement(1113..1790)
                     /locus_tag="RJE42_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDV5877089.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCDCGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(1921..3027)
                     /gene="rpoD"
                     /locus_tag="RJE42_001673"
     CDS             complement(1921..3027)
                     /gene="rpoD"
                     /locus_tag="RJE42_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV5877090.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(3251..5050)
                     /locus_tag="RJE42_001674"
     CDS             complement(3251..5050)
                     /locus_tag="RJE42_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV5877091.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIARNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPVNYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINENGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(5111..5929)
                     /locus_tag="RJE42_001675"
     CDS             complement(5111..5929)
                     /locus_tag="RJE42_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDV5877092.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(5940..6563)
                     /locus_tag="RJE42_001676"
     CDS             complement(5940..6563)
                     /locus_tag="RJE42_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5877093.1"
                     /translation="MELSQRQERIIEIVKTQGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6898..8289
                     /locus_tag="RJE42_001677"
     CDS             6898..8289
                     /locus_tag="RJE42_001677"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDV5877094.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(8440..9186)
                     /gene="recO"
                     /locus_tag="RJE42_001678"
     CDS             complement(8440..9186)
                     /gene="recO"
                     /locus_tag="RJE42_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV5877095.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYSSLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEAFKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(9214..10113)
                     /gene="era"
                     /locus_tag="RJE42_001679"
     CDS             complement(9214..10113)
                     /gene="era"
                     /locus_tag="RJE42_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV5877096.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(10114..10518)
                     /gene="cdd"
                     /locus_tag="RJE42_001680"
     CDS             complement(10114..10518)
                     /gene="cdd"
                     /locus_tag="RJE42_001680"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV5877097.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSHFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(10529..10873)
                     /locus_tag="RJE42_001681"
     CDS             complement(10529..10873)
                     /locus_tag="RJE42_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV5877098.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(10876..11343)
                     /gene="ybeY"
                     /locus_tag="RJE42_001682"
     CDS             complement(10876..11343)
                     /gene="ybeY"
                     /locus_tag="RJE42_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV5877099.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPDIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(11344..12291)
                     /locus_tag="RJE42_001683"
     CDS             complement(11344..12291)
                     /locus_tag="RJE42_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV5877100.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         3844 a         2291 c         1673 g         4483 t
ORIGIN      
        1 gattttagtt taaatatatt gaaatggatc tgtattaatt gtagatgctt caacatctat
       61 atttattttt tctgtattga accaattcat tagtaacgtt tttaaacctt ctttcatcac
      121 atattcgctg taatgattaa tatcaattaa gttcacaccc tgaattttag catctaaggc
      181 atcatgatgt ttaatatcac ctgtaacaaa gacatctgcg ccttgttgaa cagcttgata
      241 ttcatatcca atacctgaac caccaataat tgcaatacgt ttaattttct gattagactc
      301 accaacaaaa cggacacttg ggatatttaa tttagattta aaatcagctg cgaaatcttc
      361 caatgtcatt tgattatcca cttctgccat aacgccaagt ccataaagag atgtttgttt
      421 tatctcaata aaatcaaata ccggtgtttc atatggatgg tattgtttaa ttaattgctc
      481 agcccttgac ttttgacatg catctatcat aaattcaatt ttaacttcat ctacatattc
      541 aattttatca atttgtccta ttgttggatt agcttcacca actggtttga attgacctct
      601 tccttcactt tcaaagaaac aatattcata attaccttct tgtgctaatc cattttcact
      661 aagcttatct ttaaatggtc caacattatc cttaggtata tatgtttgaa ctttatagta
      721 tacatcttgt tgattattta ttattgaaat gttcttcaat cccatcgcct tagccaacat
      781 catattgaca ccatgaggat ttacatctaa atttgtatgc atcgctatta aattaatgtc
      841 atgttgaatt agttttctaa taatcgaacc ataaccatta gcttttaatg atgttacgcc
      901 tttaaagatt agaggatgat gactaataat agtattataa cctttttcta tcgcttcatt
      961 tactacttcc aacgtacagt ctaatgctgt taaaacacca gtaacttcaa catcttcatc
     1021 acctattaac aatcctacat tatcccaaga ttcagcagtt ctaaatggca catgataatc
     1081 taacaatgtc attaaatcag ctattttcat tactataaca ccctttcaat tacagcaatt
     1141 tcgtcattaa tttgagctaa acgttgatga tgttgttcag tattgagttt cgatttaata
     1201 tgataaagtg cttctaactc tctttgccat ttttttataa aatattcatt tttgttgttc
     1261 aataattttg gtccgaattt caattcatca gatgatagct ctattaattg cgtagaatat
     1321 tctgctacaa caatttcata aatatggcct ttttcttcca ttattatttc atcaattatt
     1381 tcataattca attgttgtaa tgtttgtctt aaattttcag tttggatatt actttgtaaa
     1441 atcaaccttg gatgttgact taacttatct tgcccatctt ttaaaatttt agcaataagt
     1501 ggtccgccca taccacaaat tgtgatatta tcgattacat cctcaggatg aataacactt
     1561 aagccatccc ctaaacgtac atcaattcta tctactaatt gatttgcagc tacatttttc
     1621 acagcagctt gaaaaggacc ttgaataact tctcctgcaa taccgcaatc gcataaatgg
     1681 ttttgaattg catagattgg caaataagca tgatctgagc caatatccgc gattgtacct
     1741 tgttttaaaa atcgacttac cgtcgttaat cggttattta acgaaatcat tttcttctcc
     1801 tttatgaaaa aagaggttga acattattca atgtaccaac ctcaatcaag ttaaatctta
     1861 atctaaaaat aaataagcgc ttattcatgt cttggtatca atcattaaaa ttgatataaa
     1921 ttaatccata aagtctttca aacgtttact acgacttgga tgtcttaatt ttctaagtgc
     1981 ttttgcttcg atttgtcgaa tacgttcacg tgtaacaccg aaaactttac caacttcttc
     2041 aagtgttctt gttctgccgt catcaagacc aaatcttaat cgtaatacat tttcttctct
     2101 atcagttaat gtatcaagca catcttctaa ttgctctttt aataattcat aagcagcatg
     2161 atctgaagga ctttgtgctt cctgatcctc aataaagtct cctaaatgac tatcatcttc
     2221 ttcaccaatt ggtgtttcta atgaaacagg ttcttgcgca atttttaaaa tttcacgaac
     2281 tttttctgct ggtaaatcca tttcttcacc aatctcttct ggtgctggat ctcgacctaa
     2341 gtcctgtaat aattgacgtt gaacacgaat taatttatta attgtttcta ccatatgcac
     2401 agggatacga atcgtacgtg cttggtcagc aattgcacga gtgattgctt gtctaatcca
     2461 ccatgttgca tatgttgaaa acttaaatcc tttgttaaag tcaaattttt caacagcttt
     2521 aataagaccc atattacctt cttggattaa atcaaggaat aacataccac gacctacgta
     2581 tcttttagca atacttacaa ctaaacgtaa gttcgcttct gcaagtcttg attttgctac
     2641 ttcatcacct tgttcaatac gtttggctaa ttcgatttct tcttgtgcac ttaataagtt
     2701 aacacgccca atttctttaa ggtacatacg aactgggtca tttattttaa cacctggagg
     2761 ggcactaaga tcacttggat tcagtttctc gtcagtatct gaactatctt tttcattaac
     2821 tagtgaaata tcattatcat ttaattgatc aaagaaatca tccatttgat cagagtcgat
     2881 atcgaaattc tgaagttttt cagcaatttc ttcatgactt aaatgaccct cttttttacc
     2941 tttttcaatt aattgcttct taacatcttc taatgttaat gtcggatcaa ttgtttgttt
     3001 tttaatttta actgtgttat cagacatgaa acggcctccc gattttaaat atgaacattc
     3061 gaaatttatt caatattgct attttaaact aaattcttaa ttaattcatc catattttta
     3121 attttatttt acaaattgga actaatccca atatttattt ttcaatagtg ttgtttaaat
     3181 gcggtaacta tcatttaccc ttatacattt tgacataatc attattcgta gtattcttta
     3241 aaatcacatg ctacatgcgt tctttattct tagcaacaat ttgctgtaaa tagtatttct
     3301 gtaattctac atcgccaatc cttgtagctt cccttaattt atgattcaat gactcaattg
     3361 tttcttgtcc gttttcatta ataacattga cataatcatc aatttcattt tcatatggtt
     3421 catcattcaa actatattgt tctaagctaa ttagtgtttc tctcaactca tttgaattaa
     3481 catactgcac agcatcactg atattatatt gatcattttc cgcataaaaa tcatgtaaga
     3541 cttcgaatac atatttaaaa tgctgatttg tgaagttatc cttatcaaca ctttcataat
     3601 aatttaaaaa tgtatcttta tctctcatta aatgttttaa aaatgctcgc tctgcttttt
     3661 cttgccggct caaattgtca aattgtgcca taccaattgg ctcaggttca atgtaaccgc
     3721 catactcatc ttctggataa taattgactg gtgcttgatt gaattgtatt tcgttagcta
     3781 attgctcagg actaacattg aaaaatggtg caacatcatt taaagccttt tgttgcaaaa
     3841 tcgatgactt cataagtgaa atgtcatgac tcagttcttt caaataacgt tcatatgaaa
     3901 ggtcattacg tgcaatttca tcttttaata tactcacttt ataatgtgca aatgactttt
     3961 tatcattttt tacaaaagca gtaaatgcgt cgttgccata cttaccaatg tattcatcag
     4021 gatccatgcc agatggcaat tgtataacaa atacatttag cccttgctgt aacaaatgtt
     4081 gacctgtttt aagtgttgct tcactacccg caaaatcccc atcaaacatt aatgttatat
     4141 ttgatgttaa ctttcgtata aaggtaatat gttcatctga caactgtgta cccattgttg
     4201 caacaacgtt tttcaagcca gcagtatcag attttataac atccataaaa ccttctagta
     4261 atacaatttc atctaatttt ctaattgatt tacgtgcttt atctaagtta tataacaact
     4321 ttcttttttg aaagataggt gtttcaggac tatttaagta ttttggttct tgaccggtat
     4381 atgttcgacc tgaatatcca acaattcttc cttgcgcatt tttcaaagga aacataatac
     4441 gatttcgaaa tctatcgtaa taactgaagt tttcttcgtt acgtgataat aatccggctt
     4501 catatgctaa ttcaatatcg taaccctttt tttgaagaaa atcatgacaa aaatgtgagc
     4561 tatcgggtgc aaagccaatg cctcgctctt taataagcgc atctgtaaaa ccacgttctt
     4621 gtaagtacgt taatgcttgt tcgccttcga ctgtctttgt taaagcgtaa tagtaaaatt
     4681 cttgtattaa ctcatgcatt tcaatcattt gtaaatcatc agaagcaatt tgaacatttg
     4741 agttagattg tgttgcctca atatctacag caacattaac tctatcacct aattctttaa
     4801 ccgcttcaac aaatgatatg tctttaattt cttgagtaaa ttgaaaaaca ttgccacctt
     4861 ttttacaacc aaaacaatgg caaatttgtt tatcttcaga aactgtaaat gaaggtgtct
     4921 tttcatcatg aaaaggacac aaacctatat aattgcgtcc tctcttttct aattttacat
     4981 attcacttac caagtctaaa atgtcggttt tatcttttat ttcattaatg atcgattgat
     5041 ctattcgcaa attaaatcac ctattaagca ttagttatac tattatacct aatttttgac
     5101 aaaaatgaaa tcatttcgat ttattttgtt caatataatg gattatatcg tttgctgttt
     5161 cttcgattgc tttttgagaa acatcaatga caggacatcc aatttcactt acgatttctt
     5221 caaagtaatt caattcttct tgaattcgtg cttctgttgc atatcgagct gtgtcaccta
     5281 gtcctaattg ttttagtcgc tcttttctaa tgcgatttaa tttctcttca cttattttaa
     5341 gtgcgataca tttctttgga tcaatatcat ataagccatc tggcggtgtc acttctggta
     5401 caatcggtac attcataact ttgtaacttt tatgcgctaa atactgggat aatggtgtct
     5461 ttgaagttct cgaaatacca agtaaaacaa tatcagcttt aggtaatcct ttaggatctt
     5521 taccatcatc atatttaact gcaaactcta tcgcatcaat tttcttgaaa tatgcatcat
     5581 ctaatctatg aacaatacct ggctcattat aaggtttttc ttcaaccgat gctgataata
     5641 aatccattaa tggccccata atatcgacgg acttcaattg gaattctgct actttctcac
     5701 tcatatattg cttcatttca ggtttaataa gtgtataaac aatgatagca tttgtatctt
     5761 tagcaacttg aatcacttca tcaacatctt caaaagattc aatatatgga tatcttaata
     5821 attcattttt acattgctta ggattaaatt gtgaaatacc tgccctagca actaactctg
     5881 ccgtttcacc tatagaatct gaagctacga taattttaat tttttccatt acttctcacc
     5941 tattctttat ataatgctac taataactta gctattgttg ttttggaaat tcttccaatt
     6001 acttcatact tttgattatc tttttttctt acaattggaa tcgaatcaat ttctttttca
     6061 atcattctat ctgctgcgta tatgactaat tcgctttcct ctaaataagt gacattaggc
     6121 atacgtgtca tatttacact gataggtact gtatgaatat ctgctccaat cattgaagct
     6181 cttaataaat cttttcttga acacacacca acaaaatcgt tatcttcatt aataataaat
     6241 aatgtactta catcttctaa aaaaattgta caaatagcgt cataaactgt tgtattctct
     6301 cttagcacaa caggttgaga catatagtcc ttaacctcaa attgtcgaag tttttcatta
     6361 aaaaatttac cttttgattt acctgaataa taatatccaa ctcggggacg cgcttctaaa
     6421 aaacctgaca ttgttaatat cgctaaatct ggtctgagcg ttgctcttgt taaattaagc
     6481 ttatctgcta tttgttcacc agtaatgggt ccttgagttt taacaatttc gatgattcgt
     6541 tcttgtcttt gactgagttc tataggtctt cacccctttt ttattcactt attattatac
     6601 cgatatttct ttcatgtcac aaacacatta attttacttg cctttaaata atctatcaat
     6661 tagaataaat gtacagcgag ttaaggatag tgtaagctta acaataagat tggcgcaacg
     6721 aatcatttta aaataaaagc gagtgactac actaatttgg gtggaaccgc gggttaaata
     6781 cgtaacttat tttgtattat atacaattta aaatattaac tcgtcccatg gaagtaaagc
     6841 atatttacta tgctgttatt ttccatgggg cgttttcatg tatgaggaga ggtaattatg
     6901 gcaaaagata tggatacaat tgtttcatta gcaaaacaca gaggttttgt gttccctggt
     6961 agtgatattt acggtggttt atcaaacaca tgggattatg gtcctttagg tgttgaatta
     7021 aagaataatg ttaaaaaagc ttggtggcaa aaattcatta cacaatcacc gtttaacgtt
     7081 ggtatcgatg ctgcaatctt aatgaatcca aaagtatggg aagcttcagg acacttaaac
     7141 aacttcaacg acccaatgat tgataataaa gatagtaaaa ttcgatatcg cgctgataaa
     7201 ttaattgaag attatatgca agatgttaaa ggtgatgaaa acttcattgc cgatggttta
     7261 agttttgaac aaatgaaaaa aattattgac gatgaaggta ttgtttgtcc tgtaagtaaa
     7321 actgctaact ggactgaaat tcgccaattc aatttaatgt ttaaaacatt ccaaggtgta
     7381 actgaagatt ctacaaatga aattttctta cgtcctgaaa cagcacaagg tatttttgta
     7441 aactataaaa acgtgcaacg ttcaatgcgt aaaaaattac catttggtat cggtcaaatt
     7501 ggtaaatcat tccgtaatga aatcactcca ggtaacttca ttttcagaac aagagaattt
     7561 gaacaaatgg aacttgaatt cttctgtaaa cctggtgaag aaatcgaatg gcaaaattat
     7621 tggaaaactt ttgcaagtga ctggttaaca agcttaaata tgagtagtga aaatatgcgt
     7681 ttacgtgatc atgatgaaga tgaattatct cattactcaa atgcaacaac tgatattgaa
     7741 tataaattcc catttggttg gggtgagtta tggggtatcg caagtcgtac agacttcgac
     7801 ttacgtaaac atgctgaaca ctctggtgaa gatttcagat accacgatcc agaaacgaac
     7861 gaaaaatata ttccatattg tatcgagcca tcacttggtg cagatcgtgt aacattagct
     7921 ttcttatgtg atgcatatga tgaagaaggc gttgaaggta gtaaagatgc acgtacagtt
     7981 ttacacttcc atcctgcatt agcaccatat aaagcagcga ttttaccttt aagtaagaaa
     8041 ttatctggcg aagcgattaa gatttttgag caattaagtt ctaaattctc aatcgatttc
     8101 gatgaatcac aatctatcgg taaaagatac cgtcgtcaag atgaaatcgg tacaccttat
     8161 tgtgtaacat tcgactttga ttcattagaa gataatcaag ttacagtacg tgacagagat
     8221 tcaatggaac aagttcgtat gccaatctca gagttagaag ctttcttaac tgaaaaaaca
     8281 aaattctaat taaaaagact ctccaccata tgtggtggca atataattta taaaaagaga
     8341 tactggttag taaaattgcc agtatctctt ttctattcga aaaaggtatt caaataaaca
     8401 catctatgtg gctacttgaa taccttttat tattttatgt tattgttcca atctttttaa
     8461 ttggttgatt agtttctgac ttttaaaaaa catacctgca tattcacgat ataacattaa
     8521 aatgatatct gacatttcat caataatttc ttgatggata ttcaatgaat tcattttatc
     8581 tattggtaat ttttgtaata catctaataa atatagtgtt ttattcgata atataactgc
     8641 atgtacatct ttaaaagcct cttgccttga aatcgcaccg tcaaacttaa aactataacc
     8701 tattaaatct gcttgtgtgt cattaccact cacagcacag cgattaaatg atgcggtaaa
     8761 accaaatcgt ttcatacact ttaacataac aacgactgac attaactgtg cagatgtacc
     8821 tgattctatt ttttcaagaa caaattgtaa taattgatag ttatatggtg caatgtcacc
     8881 ttcatccatt gagcgctcaa tagtttcagc tgccagagac gaataactgc ttacgtaaag
     8941 gtccatttgt aatttataat gttgactaat aacatctaca gaatttaacg ttcccatacc
     9001 tcgccattga ttgtaaataa acaaaccata aacaaacaat tgcgtttgcg cttgtaaacc
     9061 cgtcttaact tttttagcac gtctagccat aagtggtact tttgcaccat gctcatttaa
     9121 aatcgtaata attttatcag attcaccata atcaactgct ttgatgataa tccctttttg
     9181 gcgcattaac aattatcttc accactttta agattaatct tggtcttcaa cataaccaat
     9241 ttggcgaata aagttaactt tgtttcgcca gtctctttga actttgaccc ataattctaa
     9301 gtaaacttta gagcctagaa gcatttctat atcacgtctc gcacgttttc ctacttcttt
     9361 taacttttta ccgccttttc caatgacaat tcctttttgc gaatctcttt caacatatat
     9421 agttgcttcg atatgaacac gatcttcgct ttctttaacc atacggtcca cattaacacc
     9481 aatcgcatga gggatttctt cacttgtaag atgaaggatt ttttcacgaa tgatttcacc
     9541 cactacaaat tgttcaggat ggtctgaaat ttgatcatct ggataatatt taggtccttc
     9601 gggtaaatac gtctttaata catcaataaa atgatcgaca tttagccctt ctaatgctga
     9661 aataggtaca atctctgtaa aatccatata actttgatat tcttcaatct ttggcattaa
     9721 ttcatctgga tgcactaaat ctattttatt taatactaaa aatactggtg tcttaacatt
     9781 tttcaacatt tctataatat attcatcgcc tcgtccaatt tcctcattgg cattaaccat
     9841 aaacatgatt gcatctatct cagataatgt atttttagcg actttcatca tatagtcacc
     9901 taatttgtgt ttaggtttat gaatacctgg cgtatcaatg aatattattt gcgcatcatc
     9961 tcttgtcatt acaccttgaa ttttatttct agttgtttga gctttatcgg acatgattgc
    10021 tattttatgg ccaatcactc tattaacaaa tgttgacttc cctacatttg gtctacctat
    10081 aattgaaaca aatcctgatt tatgttctgt catttattct aaatcctttc ctgaaaatcc
    10141 aaatggtagt aactctgcga ctgtcatcat aaccatatct cctttatgat ttgtcatata
    10201 cacaggcata tcatcatcac ataattcctt caaaacttga cgacatgcac cacatggtga
    10261 tgacggttta tctgcatcta cggttacagt tattgattca aaatcacctg gtctgtatcc
    10321 ttgagaaatt gccgatacca aactagctcg ttcagcacat atcgataatg gataagaagc
    10381 attttctaca ttggtaccat aaaaagttct accgtctttc gtttttaaat aagcccctac
    10441 tttaaaatga ctgtatggcg aatatgattc ttgttgtgct tttctaactt cttgaaaata
    10501 atgaggttga taactcatat atgcctccct aaaataacgc tataaaatgt ggtaaaaata
    10561 ctattaaacc tataataaat gctaatattg aaactataag tacactaaaa gccgctatat
    10621 ctttagcgta tttagctaaa tcatgatatt caacggtcac taaatctaca acatattcaa
    10681 tagcagtgtt taaagcttca acagtgagaa ctaatgcaat agcaatgagt ataaatatcc
    10741 actcaatccg attaatattt agtacgagac caaagacaat agcaacaatc attgcaaaca
    10801 catgtaaaag aaatttatag tctttttgaa ttaagatttt cagcccatca agtgcatatt
    10861 taaacctttt cataattagt ctcgtgttaa tccatatgcg tttaatattg tatcttgtcg
    10921 accaaacatt tccttttcat ccgcttcagt catatgatca taacctaata gatgcaaaaa
    10981 tccatgtaat gctaaaaatc ctaattctcg ttcaaaagaa tgtccgtaat tgtttgcttg
    11041 ttcttgcgct acatccgtac agataattat atcccctaaa acacgtggta tatcaagacc
    11101 actaaaatca atatctggct catcttcttc taaagcaaat gagattacat ctgtaacttt
    11161 atctttatct ctatatgttc gattaatttc ttgtatttct tgtttatcta caaatgtaac
    11221 agaaagctca gcatcgtctt ctatatgctc ctctttttta gcaaattcta ataaatcttc
    11281 aatttgttta taccaagcat ctttaactaa gcctgtgtga tcgctaaaat ctatcgtaaa
    11341 catttaattc tctccttcat aatgttcaat gattttactt accaatggat gtcttactac
    11401 atcgctctga tctaatttca atatacttat acctttaacg ttgtgtaacc tactgaccgc
    11461 ttccttaagt ccacttttaa cacctttagg taaatcgatt tgagtttggt caccagtaac
    11521 taccattttt gagccaaaac ctagtcttgt taaaaacatt ttcatttgcg catgtgtcgt
    11581 attctgcgcc tcatcaagaa ttacaaatgc atcttctaat gttcgtccgc gcatatatgc
    11641 aagtggcgct atttcgataa tgcctctttc aataaatcgc tctgtttgtt cacgcccaag
    11701 aacagtatat aaaccatcat ataaaggtct taaatatgga tctacctttt ctttcaaatc
    11761 tcctggtaaa aatccaagtg actctcctgc ttcaacagca ggtcttgtta atacaatacg
    11821 tttaacagca cctttacgga gttgctttgc tgcataaact acagctaaga atgtcttacc
    11881 tgtaccagca ggacctatac caaatactaa atcattattt ttcatggcat taacatatat
    11941 acgttgcccc atcgttttcg cacgaatcgt cttaccaaat gcatctttag ttatctcttc
    12001 atcatataaa tctaacagat gttgaattgt gttattatgc gccattttaa tagctgcttc
    12061 aacatcttta attgtaatgt tattacctaa atcaataacc ttcagcaaat tgattaatac
    12121 tgattccgct ttttctacgt tttctatttt tgtaccttta acggcaactt cttgtcctct
    12181 tgcatggatg acaacatcga aactctcttc aattgctttt aaatgttcat cattatttcc
    12241 aattaaagct tgagattggt tcatatcgtc tatttgtata attccaggca t
//
LOCUS       DAPIDM010000072        12259 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000072 DAPIDM010000000
VERSION     DAPIDM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.072"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(168..1574)
                     /gene="gndA"
                     /locus_tag="RJE42_001684"
     CDS             complement(168..1574)
                     /gene="gndA"
                     /locus_tag="RJE42_001684"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV5877101.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(1642..2775)
                     /locus_tag="RJE42_001685"
     CDS             complement(1642..2775)
                     /locus_tag="RJE42_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDV5877102.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMKSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNETVVKIAQESASNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            3038..3217
                     /gene="prli42"
                     /locus_tag="RJE42_001686"
     CDS             3038..3217
                     /gene="prli42"
                     /locus_tag="RJE42_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDV5877103.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(3344..4324)
                     /locus_tag="RJE42_001687"
     CDS             complement(3344..4324)
                     /locus_tag="RJE42_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDV5877104.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDVHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLVELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(4338..4775)
                     /locus_tag="RJE42_001688"
     CDS             complement(4338..4775)
                     /locus_tag="RJE42_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDV5877105.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(5125..6399)
                     /locus_tag="RJE42_001689"
     CDS             complement(5125..6399)
                     /locus_tag="RJE42_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDV5877106.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASSTVEQTSTSKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDLMSVI
                     ENGGTTAQSDKQVQTQSTSVDTSSNQSSEGNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNKFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(6412..7395)
                     /locus_tag="RJE42_001690"
     CDS             complement(6412..7395)
                     /locus_tag="RJE42_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDV5877107.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(7395..8387)
                     /locus_tag="RJE42_001691"
     CDS             complement(7395..8387)
                     /locus_tag="RJE42_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDV5877108.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRSKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARERAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(8403..9824)
                     /gene="lpdA"
                     /locus_tag="RJE42_001692"
     CDS             complement(8403..9824)
                     /gene="lpdA"
                     /locus_tag="RJE42_001692"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV5877109.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVLQTVKQAAMFGVDVKDVNVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(9977..11656)
                     /gene="recN"
                     /locus_tag="RJE42_001693"
                     /pseudo
     CDS             complement(9977..11656)
                     /gene="recN"
                     /locus_tag="RJE42_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
     gene            complement(11672..12124)
                     /gene="argR"
                     /locus_tag="RJE42_001694"
     CDS             complement(11672..12124)
                     /gene="argR"
                     /locus_tag="RJE42_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV5877110.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
BASE COUNT         3733 a         2357 c         1686 g         4483 t
ORIGIN      
        1 attttattac ttcacaaaat gcttaaaaat atggcgggat gtttttaagg tcaaagatat
       61 tgcttgtgta attttttata ggttgtagct actctatcac actctctttt atatttatca
      121 aaagatataa aaaaggatag tatctttcaa ctatccttta atcaatatta ttcttcaatc
      181 cattgtgtat ggaatacgcc ttctttatct tttctttcgt acgtgtgagc accgaagtag
      241 tcacgttgtg cttgaattaa gtttgcaggt aaatcagctg cacggtaact atcatagtaa
      301 ttaatacttg aagagaaacc aggtgttggt acaccatttt gaacaccagt tgcgacaaca
      361 tcacgtaacg catcttgata ttcagtaacg atgtttttaa agtaaggatc tagcaataag
      421 ttttgtaatc ctggattatt atcgtaagca tctttgatct tttgtaagaa ttgtgcacgg
      481 ataatgcaac cttctctcca aatcatagct aaatcaccaa gttttaaatt ccattcatta
      541 tcttcacttg ctttacgcat ttgcgcgaaa ccttgtgcat aagaacaaat tttactcata
      601 tataatgctt tacgaatttt ttctaaaaag tctttcttgt caccatcaaa tgatgctttt
      661 ggaccattta attctttaga agcatttacg cgctcttctt tgattgaaga gataaaacgt
      721 gcaaatacag attcagtaat gattgttaat ggaataccta attctaatgc gttaattgaa
      781 gtccattttc ctgtaccttt ttgacctgca gtatcaagaa ttttttcaac taatgcttct
      841 ttattttcat ctaatttcat gaaaatatca ccagtgattt caattaaata actttctaat
      901 tcaccagcat tccagtcttt gaacgtttga gcaatgtctt catgagacat gcctaataat
      961 tctttcatca tagcataact ttctgcaatt aattgcatgt cagcatattc gataccattg
     1021 tgtaccattt tcacatagtg tccagcacca ttaggtccaa tataagtaac acatgaagca
     1081 ccgtcttttg cctttgcagc aattgcatca agaatatctg caactttgtt ataagcttct
     1141 tcttgtccac ccggcattaa tgacggacca gttaacgctc caatttcacc accagaaacg
     1201 cccataccaa taaagttgat tgcactttgt gctaatgctt tattacgtct gatagtatct
     1261 tgatagtttg tattaccacc atcaattaaa atatctccat catctaataa aggtaacaaa
     1321 ctatcaatcg ttgcatccgt agctttacct gcttgaacca ttaataaaat tttacgtggt
     1381 ttttctaaag aattaacaaa ttcttccaat gagtacgttg gatgaatatt ttttcctttt
     1441 gattcttcaa ccattaaatc agttttttca cttgagcggt taaatacaga tacactatat
     1501 ccgcgtgatt caatattcca agctaggttt ttacccataa cggctaaacc aataactcca
     1561 atttgttgtg tcatattact tacctcactt gttgattttt cattagtatt gtatcacaaa
     1621 atagacatac actacactaa atcatttcga ttgtcgcgca actattttga ttatttctaa
     1681 cacttgactt gcaagcaagt tcaatgattt aatcggcatt ctctcatttg ttgtatggat
     1741 tttttcataa cccactccta aaatgactga aggaatacca aatgtattaa taatactgcc
     1801 gtctgaaccg ccaccagaaa taattgtatt tgcagataat cctaaattac tagcactttc
     1861 ttgtgcaatt ttaacaaccg tttcattatc attaatttta aatcctggat aactttgctc
     1921 cactgtaact actgctttcc cacctaattc tgatgcagta gtttcaaaca catcagtcat
     1981 atgtttgact tgtgttttta ttctttctgg atcgtgagaa cgtgcctctg cttctaagat
     2041 gacttcatct gcaacaatat tcgtagctga accaccatga aacttaccaa tattggcagt
     2101 agttatttca tcaacttgtc ctaatttcat tcgactaatt gctttcgccg cgatattaat
     2161 agcactaaca ccctcttttg gcgtacttgc atgagccgtt ttgccaataa ttttagctga
     2221 aattaacatt tgcgtcggtg cacctacaac cgtagtaccg acatcagcac ttgcatcaat
     2281 agcataacca aagtccgcgt ccaacaactc tgaatttaat tctttagcac caattaaacc
     2341 tgattcttct ccaacagtaa tcacaaattg aatttgtcca tgtgggattt gttgttcctt
     2401 tatcacttgc aaaacttcaa gcatagctgc taatcctgct ttatcatctg cacctagaat
     2461 agtcgtaccg tcagagtata tgtagccgtc atcttttaca attggcttta cattaattgc
     2521 gggtacaaca gtatccatat ggctcgtcaa atataattta ggtacttcgc cttcttcgat
     2581 agtacttttc attgtacaca ctagattatt ggcacctaat ttaggatgtt tagccgcttc
     2641 atcttcttta acatctaacc ctaatgctat gaatttttct tttaaaatag gttggattgt
     2701 tgattcattc cctgtctcag aatcgatttg tacaagttct aaaaacgtat taagtaatct
     2761 ttgctcatta atcataatgg aacccctttt atccaaattt tcaagtacgc caaacggtaa
     2821 aaattgatga aataaaatgt tacagtaatt gacgttacac agatttatca ggcttgtaaa
     2881 ttgtgtcata ttattttcaa tttaatatat atatttattg taactcaaac taagctttgt
     2941 caaaaatata ttgattgatt tttcaaagat atcgtataat gaggaaaatg acataagcaa
     3001 acttactcat gttttttata atattccttt atgatgattg ctagttatat cgtctcaagt
     3061 taaaagtttt atatcttatg tcgtaattat taatacaaag gttattcatt tggaggcaca
     3121 caaaatgcaa aataaagttt taagaattat cattatcgtt atgcttgtat cagttgtatt
     3181 agcattgtta ttaacgagta tcattccaat tttataaact atatctcaac tacctataca
     3241 aaatcataca attaaaaatc catccgttat aaacgcatgt attaataagt tatcgtattg
     3301 caacgattac tttcaaacat gggtcatacg gatggattat tttttaagct acttcgctat
     3361 gcattttcaa tgaaccaaat tgcgatttga tttgtaaata ttcttctaat tcatttaata
     3421 tttgaataat acttgctctc gagttaagcg ctttgtgtgt tgttggcaat ggcagttcaa
     3481 ccaatttcaa acgcgtctca tacaaattgt gtaaacgcat tgctgtatag tcattactat
     3541 tcacatttag accaatttct ttcagcagtg acgcaacatc atttaaaagt ggatctttat
     3601 gacagatact ttcgatgagc ggtttcattc tcattaacaa ttccacttgc tcttctcgca
     3661 tatcaaaata atgatagtat gaattttcgt ttctaacaaa atgattttta acatctcgga
     3721 acgcgataga cttcgccttt ttaatattta aaagtaacgc ttcaaattca atcgcaatgg
     3781 tatcttcata tttttcacaa atataactat atttactaaa aatatcagca atttgttgct
     3841 caattttaca tttgtattcg tctagttgtt tgtctaaact tggcatcatt aaattcattg
     3901 taaatgcaat acttagtcca attaacagta ataatgtttc attaacaatt aaatgtacat
     3961 caattgattt tgcattaaaa acatgaagta atataacgca actcgtaatg acaccttctt
     4021 gtacttttaa tacgacagtt aatggtataa ataacaatac gataataccg agtacaattg
     4081 gactctgacc taataaacta aatattgctg aacctaaaaa caatactaaa aaacatgata
     4141 ctaatcttga aataatcgct tgtagcgaat gtacttttgt atgtttaata cataatacga
     4201 ctaatatggc gcttgaagca taattatcta aacctaacag cttactaata attacaccta
     4261 aagtcatacc cactgctgtt tttattgttc taaatccaat cttgtaagga tttaacttta
     4321 acatgggtta gcgcctctta tctttcttca caatatttat tgaataatgt ttgtaattga
     4381 ttaattacgt tcatcacatc atgaccttcg atttgatgtc tttcaatcat ttctgtaatc
     4441 tttccatctt ttactaatgc aaatgacgga cttgaaggcg cataaccttc gaagtactca
     4501 cgcgctcttt gtgtcgcttc tttatcttgt ccagcaaata ctgtcactag acgatcaggt
     4561 aatacgtcat aatgtaaagc atgtgatgct gctggtcttg cgataccacc tgcacaacca
     4621 catacagaat tgatcataac tagtgttgta ccatcttgtt taagaacttt gtcaacatct
     4681 tctgcagtag ttaattgctc atatcccgca gattcaattt cattccttgc ttgttctaca
     4741 acaccgttca tgtataaatc gaaattcatg tccataagtt caatcaccta tccctttata
     4801 tttaaactat cctcattcta ctaattaata acatattgtt caataaacta atctgaatca
     4861 cacctatatt tagacacaat tttaacaata taccaaacat tattgtgctt aaaatcatgg
     4921 aaactaattt gttcacctgt tttcattaat atgtttcaag tatgatgtct tattttgact
     4981 ttactgcaaa aatgcattca accatgttga ttattgttct ttaccttttt tgaatatatt
     5041 gcaaatattt tattgcccaa aaaaatacat ccatcgacaa gaacaagata aaacaagttg
     5101 tcgatagatg catctttgtt atcactaata tatatttgta ttttctaaag tatactgttc
     5161 gatacgctgt ttaatatgat tcataaattt acctgtttgt aaaccatcta aaatacgatg
     5221 atcaattgaa atacataaat taaccatgtt acgaattgca atcatatcat taattactac
     5281 tggcttttta acgattgatt ctacttgtaa aatcgccgct tgaggatgat ttataatacc
     5341 cattgatgat actgaaccaa atgtaccagt attatttacc gtaaatgttc caccttgcat
     5401 atcttcagtt gtcaattgct tattacgcgc ttttgttgct aaagtattaa tttctctagc
     5461 tatacctttg attgactttt cgtctgcatg cttaatcaca ggtacgtata atttattttc
     5521 atcagcaaca gcaattgaaa tattaatgtc tttatgtaag acaatttcat ttccttgcca
     5581 gctactattt aataaaggat atgcttttaa agcatctgct acagctttta caaagaaagc
     5641 aaagaaagtt aaattatatc cttctttatt tttaaatttg tttttataat ggtttctcgt
     5701 attcacaagg tttgtagcat caacctcaat catcatccat gcatgtggaa tctccgttac
     5761 actattaacc atgttttgtg caattgcttt acgcacacca tttactggta ttgtgctatt
     5821 ttcactattg ccttcagatg attggttact tgatgtatct actgaagttg attgtgtttg
     5881 aacttgtttg tcagattgag ctgtggtacc accattttca ataactgaca ttaaatcctt
     5941 cttagttaca cgaccttcaa atccactacc tacaacttgt gataaatcaa tgtcatgctc
     6001 tgaagcgagt ttaaatacaa caggtgaaaa gcgaccatta tttcgtggtt gattttgttt
     6061 agaagtagat gtctgttcca ctgttgaact agctttttta gtagatttct gagtatgctc
     6121 atccactttt gcttgtatct cttcagttgt ttcatttgtc ttttcatcag cagtttcaat
     6181 tttacagata attgtatcaa tagctaccgt ctgccccgct tcaactaaaa tttctgtaat
     6241 tgttcctgat atcgtggaag ggacttcagc tgtcacttta tctgtaataa cttcacataa
     6301 tggttcatat tcatcaatat gatcaccaac agaaactaac cattgttcaa tggtaccttc
     6361 atgaacactc tcacctaact taggcattgt tatttccatg actttccctc cctagaattc
     6421 tgctaattca cgcattttat ttaagatttt ttctggattc atcataattt cattttctaa
     6481 tacaggtgaa aatggcatag atggtacatc tggagcagcc aaacgcatga ttggtgcatc
     6541 taaatcgaac aagcaatgct ctgcaataat cgctgacact tctgacataa tactaccttc
     6601 taaattatct tcagttacaa gtaaaacttt acctgtatgt ttagcacgat caataattgt
     6661 ttctttatct aatggataaa cagttcgtaa atcaacgact tcaacattga taccgtctgc
     6721 agctaaaata tcagcagctt gtaagcaata attgaccatt aatccataac aaaatactgt
     6781 taaatcttca ccttcacgtt taacatctgc ttttcctaaa ggtacagtgt aatattcttc
     6841 tggcacttct tcctttaaga aacgataagc ttttttatgc tcaaagtaca atactgggtc
     6901 atttgattcg atagatgata ataaaagccc tttagcatca tacggtgtgg aaggaataac
     6961 aattgttaaa cctggtgatg aagcaaatat actttcaata ctttgtgaat gatatagtcc
     7021 tccgtgaaca ccgccaccaa atggtgcacg aatcgttaat gggcattgcc aatcattatt
     7081 tgaacgataa cgcattttcg cagcttcact aataatttga tttgtcgcag gtaaaataaa
     7141 atctgcaaat tgaatttctg caattggtct tttacctacc atagctgcac caatggcagt
     7201 tccaacaata tttgactcag ctaatggcgt atcgataact ctgtcttcac catatttttg
     7261 ttgcagtcct tgagtagtac caaatacgcc acctttttta ccaacatctt caccaagaat
     7321 aaatacatct ttattttgtt gtaatgctaa gtcttgtgcc tggcgaatcg cctctaaata
     7381 agataattta gccattagtt aagactccct tcttcgtaca caaatgcata ggcttcttcg
     7441 acacttggat atggcgcgtc ttcagcagcc tttgtcgctt tattgatgat gtctttatgc
     7501 tccgcttcta tttctgccaa ccaagcatca tcgataatgc cagctgaaag caactctttt
     7561 ttgaattttt cattgcagtc tgctttttta agcgcttcac gctcttcttt cgtacgatat
     7621 tgatcgtcgt catccgatga atgagctgtc atacgagaag ttactgcttc aatcaatgtt
     7681 gaaccctgtc cagaaatagc tcgttctctt gcttctttca tcgctttata cattgctaaa
     7741 ggatcattac catctacttg ttcaccatgt ataccgtaac caagtgctct atccgataat
     7801 ttttcagctg cgtattgtaa tgaatcaggt actgaaattg catatttatt atttataatg
     7861 acacatacaa aaggaagttt gtgtacaccc gcgaagttta aaccttcatg gaagtcacct
     7921 tggtttgagc taccttcacc aacagttgct gttgcaattt tcttcttacc atccattttt
     7981 aaagctaaag cagcaccaac agcatggggt atttgagttg ctaccggtga actttgtgac
     8041 aaaatattct tagatctact actaaagtgt gatggcattt gttttccacc agagttaaca
     8101 tcgtctttct ttccaaacgc tgataaaaac gtatcatacg ctgagatacc catataagta
     8161 acgaaagcta gatctctata ataaggcgca gtaatatcac cttcttctaa tgcgtatgcc
     8221 atcccaatct gagttgcttc ttgtccttga ccacttacaa caaatggaat tttacctgca
     8281 cggttcaata accacagtct ttcatctatt tttctaccta aatccatcca tttatatatt
     8341 acttttaggt cttcttcgct aaggcctaat gatttataat caatcatgtt aaatcctcct
     8401 atttatacgt gaatagctct actttctgct ttcaatccta attccatcaa cacttcagag
     8461 atggaaggat gtgcgtgtgt tgttagtcct aattctaatg ccgagccatt catgaactgt
     8521 aacagtgatg cctcattaat caattctgtt acatgtggac caatcatatt aatacccaca
     8581 atttcttcag ttgattgatc aatcaccatt tcgctatacc cttcgtttgc gtcatggcta
     8641 tcaatcactg ctttaccaat tgctttaaat ggtactttaa aacttttaac tttcattccc
     8701 tctgcctttg cttgttcaat gtttaaacca atagaagcaa tttcaggttg tgaataaata
     8761 cacttaggca tcatgttata gtttactggg attgggttcc cttcaaacat atgatcgaca
     8821 gccacaacac cttcttttga tccaacatgt gccaattgta attttccaat acaatcacca
     8881 gctgcataaa tatgtttatc ttcagtttgt tgaaattcgt tcgttaaaat atgtcctgat
     8941 gttgaaagtt ttattttagt gttgtttaaa ccaatatctg atgtgttagg ttttctacca
     9001 atcgatagca acactttatc tactttaatt atgtctgagg aaatttcaaa tgtaacacca
     9061 tcttcgttaa cattgatatc attttcagaa agttttattc cctcatagaa tttaacacca
     9121 cgtgctgaca atgatttttt taatagttgt gaagcttgtt tactttcagt tggtaaaatt
     9181 ctttcacctg cttctataac tgttacgtca acacctaaat ctatcatcaa tgatgcaaat
     9241 tccattccga taacaccacc accaataata ccaatacttg atggtaacgt ctttaatgat
     9301 aatatatcat cgctagataa aattttatca tgatcaaatg ataagaatgg caactctgca
     9361 ggcgaagaac cagttgcaat taatacaaat tggttgggta ataagtctga ttcaccatct
     9421 tcatattcga cagaaattgt gccactttga ggtgaaaata tagatgtacc tagaatacgt
     9481 cccgtgccat tataaatgtc aatgtgattg tgttgcatta aatgctttac accttgatac
     9541 atttgattaa taatgtcttc ttttcgtgcc aacatatttt caaaattaac attaacatct
     9601 ttgacatcaa cgccaaacat tgctgcctgt tttactgttt gcaatacttc agcagattta
     9661 agcagcgatt tagtaggaat acaaccttta tggagacaag tacctcctaa tagttgtcgt
     9721 tctactattg ccactttttt acctaattga gacgcacgta tcgcagcaac atatcctgca
     9781 gtacctccac cgagaacgac taaatcatat tgtttctctg acatgttctt actcctaact
     9841 aatgatatat atccattgaa aaatttatta atacatagtt ttcatgtcca ttaattacct
     9901 attttacatg attgtctatt tagtttgaat gcacataaat aaatccataa atgagtagtc
     9961 aacacacttt tggaaattat ctacgtcttt gattgtgttg tatcatttct cttgcatttt
    10021 cacgagttaa atcagtaaca cttgcacctg aaatcattcg tgcaatttca tctactttat
    10081 catcgccaat taactcttga acttgtgttg ttgtacgatc atcttttgat gatttcgaaa
    10141 ttaataaatg atggtcgctc atcgatgcaa cttgtggcaa gtgagatata caaataactt
    10201 gtatatattc tgctatatct cgcattttct ctgccatttt ttgtgcggct tgtccagata
    10261 cacctgagtc aacctcatcg aataaaattg cagtttgacc tctcgattta acaaaaatac
    10321 tttttaacgc taacataatt ctagaaagtt ctccacctga cgcaatttta tttaaacttt
    10381 ttaatggttc tcctttatta ggactgatta aaaattctac aaattcgatt ccatcaatat
    10441 tcggttcttc taatttttta aatgaaatct caagatttgc atctttcatt tgtaagtttt
    10501 gaatttcaga tacaatgtgg tctcttaact ccctagcaac tatacgacgc tgctttgata
    10561 acgcttgtcc aacctctata acttgattat acaatgcatt tatttcttct cgtaactgcg
    10621 atgtactttg ttcatagttt tcaattttat tgatttcatt attaagcttt tcttgatatg
    10681 cgattaattc tgaaatatct tttccatatt tgcgttttaa attattcagc aaattcatac
    10741 gagattcata ctcatttaac acttgttcgt cgaattctgt attagccatt tcatcatata
    10801 actcatgttt tgcatcttct aaaatgtaat aaaattgatc aatatcttct tttaatttgt
    10861 catatttgtt tggaactata tcgtttattg ttaacaaatg gttgcttagt tcatacaaac
    10921 gatcagtgat agcattttca tccgttaatg tcatatgtgc gttattaagc gctaagctta
    10981 atttttcaga gttttgaatg cgtttaatat ctatttcaag ttgctctatt tcgccttctt
    11041 ttagatgtgc ttcagacaat tcttctaatt gaaatttcat taaatctaaa cgctgcagca
    11101 atgcttggtc tgctgattct aaatcttcta actcttgctt tttggcttta taattttgaa
    11161 aagtttggtg atatttatcc aacaaatctt gataacgtga ttctgcgtaa ttatcctata
    11221 atgttaaatg gtatttttgt ttcaacaaag attgcgtttc atgttggcca tgaatatcta
    11281 ataattcttg cataactttt cgtaaatctt gtaaagtaac tgtttgatta ttaattttac
    11341 aaagactttt accagagcta aaaatttccc gtttaactaa taaaaaatct tcatctacat
    11401 caatatccat atttttcaat atatgtatag catctttact ctcgtcaata tcaaatatac
    11461 cttcgatgac agcctttttt tcaccatgtc ttacaaaatc agatgaagct ctcattccaa
    11521 ttaattgtcc aattgcatct ataataattg acttacctga acccgtttca ccacttaaaa
    11581 cagttaaacc atcagaaaat tgaatttcta actcttcaat aatagcaaat tgcttgattg
    11641 ataaggtttg taacataaac tcatcgcatc cttataacaa attgaaaatt cttgacttga
    11701 tttcatcact tgcctctttg cttcgacaaa taattaaaca agtatcatca ccacaaattg
    11761 tgcctagtac ttcttcccaa ttgatttggt ctaatatagc tccaatagat tgtgcattac
    11821 caggtaatgt ttttagaaca agtaaattat cagtaccatc tatattaaca aaggaatcca
    11881 ttaaataacg tcccaatttt tctaaaggat ggaattttct atcatttggc aaactataaa
    11941 catattgacc tgaaggtata ggtactttaa taagttgtag ttctttaata tcacgagaaa
    12001 cagttgcttg agtgacattt aaatcataat cgtttaatcg tttaactaat tcatcttgtg
    12061 tctctatctg ttcatttgaa ataatttctc taatttttat atgcctaacc gattttttgg
    12121 gcacagtaaa cacctctgat tacgaatatt tatacattta ttttaacaca tgcactgatt
    12181 tacgactact aaacaccttt acgtaaaaag ggtaaacatg gtttatctat cttgattatc
    12241 aatctataaa tatttttca
//
LOCUS       DAPIDM010000073        12254 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000073 DAPIDM010000000
VERSION     DAPIDM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.073"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(314..673)
                     /locus_tag="RJE42_001695"
     CDS             complement(314..673)
                     /locus_tag="RJE42_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDV5877111.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(686..922)
                     /locus_tag="RJE42_001696"
     CDS             complement(686..922)
                     /locus_tag="RJE42_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDV5877112.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            1239..2303
                     /locus_tag="RJE42_001697"
     CDS             1239..2303
                     /locus_tag="RJE42_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5877113.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(2363..2686)
                     /locus_tag="RJE42_001698"
     CDS             complement(2363..2686)
                     /locus_tag="RJE42_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDV5877114.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNQ"
     gene            2787..3029
                     /locus_tag="RJE42_001699"
     CDS             2787..3029
                     /locus_tag="RJE42_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDV5877115.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(3292..4467)
                     /gene="dltD"
                     /locus_tag="RJE42_001700"
     CDS             complement(3292..4467)
                     /gene="dltD"
                     /locus_tag="RJE42_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDV5877116.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPARWFTGLVTEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(4464..4700)
                     /gene="dltC"
                     /locus_tag="RJE42_001701"
     CDS             complement(4464..4700)
                     /gene="dltC"
                     /locus_tag="RJE42_001701"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDV5877117.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(4718..5932)
                     /gene="dltB"
                     /locus_tag="RJE42_001702"
     CDS             complement(4718..5932)
                     /gene="dltB"
                     /locus_tag="RJE42_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDV5877118.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(5929..7386)
                     /gene="dltA"
                     /locus_tag="RJE42_001703"
     CDS             complement(5929..7386)
                     /gene="dltA"
                     /locus_tag="RJE42_001703"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDV5877119.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDELFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(7402..7554)
                     /locus_tag="RJE42_001704"
     CDS             complement(7402..7554)
                     /locus_tag="RJE42_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDV5877120.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(7602..7712)
                     /locus_tag="RJE42_001705"
     CDS             complement(7602..7712)
                     /locus_tag="RJE42_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877121.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(7966..8925)
                     /locus_tag="RJE42_001706"
     CDS             complement(7966..8925)
                     /locus_tag="RJE42_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDV5877122.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLATARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTKILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(8959..9738)
                     /locus_tag="RJE42_001707"
     CDS             complement(8959..9738)
                     /locus_tag="RJE42_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDV5877123.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(9738..10172)
                     /locus_tag="RJE42_001708"
     CDS             complement(9738..10172)
                     /locus_tag="RJE42_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDV5877124.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            10270..10536
                     /locus_tag="RJE42_001709"
     CDS             10270..10536
                     /locus_tag="RJE42_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDV5877125.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(10615..10998)
                     /locus_tag="RJE42_001710"
     CDS             complement(10615..10998)
                     /locus_tag="RJE42_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDV5877126.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(11128..12045)
                     /gene="lipA"
                     /locus_tag="RJE42_001711"
     CDS             complement(11128..12045)
                     /gene="lipA"
                     /locus_tag="RJE42_001711"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV5877127.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
BASE COUNT         3923 a         2167 c         1783 g         4381 t
ORIGIN      
        1 tgtgatgttc acaaaaaaga catttttaat ccaatactaa ttctatagct tttatgttat
       61 attttcaagc aagattcagt cagattgtga aaaaattaat taattttttc gtagcctgat
      121 ttcgattaaa aatgtatccg ctatcacaat catttaaaat actattaata gtcagattct
      181 taatttttaa taagcttttt aataaaacaa aatatcttta cgcaaaaata ggacataatc
      241 aaatatgtcc ctaatcgtaa ttgatatcaa tttgtatccc aaaaattggg ttaatcatta
      301 aaaagttatt ttattagcag ttttcaggat tacctgcaac ttttgcagtt ctaaatgaac
      361 taccacagcc acatgaagca attgcattag gattgtcgat ttggaaaccg ccacccatta
      421 atgattgctt aaaatcaata gtcgtaccat ttaatacggg tgcatctttt ttgtctacta
      481 aaacttttaa tccaaaatat tctaagactt catcattttc accaggcgct tcttctgcac
      541 ccataccgta tgttaaacca gtgcacccgc caccattcac tttaattttt aaatagccat
      601 ctggcatttc atttgcttta agcatatctt ttacttcgta agcagctgct tctgttaata
      661 taactgttgg catgataact cctccttaaa aaatccaagt ttcttttata tgtgcatata
      721 tattttgtaa taattcttcc ggcgaatcac cttcaacaat atcaccattt actaaagcat
      781 acaacccggc tgaacatata ccacaatgtg tcaggcaacc atactctaac acatcgacat
      841 ctgggtcatt ttccagttga ttaaaaacat aatctccacc ttttgccatg ttagagagac
      901 aaaattctac gatcggattc atacttcacc ttcttatttc atttgttaca atattatagc
      961 attttaaaac tggtatttta acatgatgtg ctcaattagc aacaactgat gtttcttatc
     1021 ccagttatgt aatagtgcct tagttaggac aaatatttat ttgtagtcat tttcaaaaaa
     1081 atatataata agcatataaa tacatattta cttttttagg ggttattgat gaatcaaaga
     1141 cacttgcatt ttataactgt tcattaacaa atatatctat gcactttgat cttttcaaaa
     1201 ttaaagtaaa tattaacata taaaaggggt acttcattat gaaaaactta gttttgttag
     1261 gcggcggata tggtaatatg cgtatcatgt cacgcatttt aactacatct ttaccacaag
     1321 attatacagt cacattagtt gatcgtatgc catttcatgg attgaaacca gaattttatg
     1381 ctttagctgc gggcacgaaa tcagataaag atgttcgcat gaaattccct aatcatccac
     1441 aagtgaatac ggtttatggt gaaattaacg acatagattt agatgctcaa attgtctcag
     1501 tcggtaattc taaaattgat tatgatgagc taattattgg tttaggatgt gaagataaat
     1561 atcataacgt tccaggagcc gaagaatata cacatagtat tcaaacactc tcaaaggctc
     1621 gggatacttt ccatagtatt agtgaactac cagaaggtgc taaagttggt atcgttggtg
     1681 ctggattaag cggcatagaa cttgccagcg aattaagaga aagtagatca gacttggaaa
     1741 tatatcttta tgaccgtggg ccacgaattt taagaaattt tccagaaaaa ttaagtaagt
     1801 atgttgcgaa atggttcgcc aaaaataatg ttaccgttgt tccaaattca aatattaata
     1861 aagttgaacc tggtaaaata tataactgtg atgaacctaa agatattgat ttagttgtat
     1921 ggacagcagg aattcaacct gttgaagttg ttcgtaactt gccgattgat ataaatagta
     1981 atggacgtgt gatagttaac cagtatcatc aagtaccaac atatcgtaac gtctatgtag
     2041 ttggtgattg tgctgattta ccacatgcgc caagtgctca gttagccgaa gttcaaggtg
     2101 atcaaattgc cgatgtgctt aaaaagcaat ggctaaatga accattacct gacaaaatgc
     2161 cggaactaaa ggtacaaggt atcgttgggt cattaggaga taaacaaggt tttgcatata
     2221 tcatggatcg cactgtaaca ggacgactcg cttctatttt aaaatccggc gtactttggt
     2281 tatataaata tcataatggt taaaaattaa aaagcaatgt cagttcacac tattactgac
     2341 attgcttttt attatgtgta tattattgat tcacaagctt ttggtctata aatcgagtga
     2401 tttgctttgt ttgtatataa ccatctgcta catattcatc gttcattgta attaatggat
     2461 aaaatagttc atcttgttct attctttcga taaattgtaa atcatgatct gttaagttgt
     2521 cattatcttt tgtaatatct atatacgtat atttaaatga tatatttggg tattttcttt
     2581 ttaatagcgg ctgtagccag tcataaatat ctttcgatgt tggcgcatta acgcaacttg
     2641 cacatataac atctgcccca tatactacca cactcacgtg ctccatatat ttatccccca
     2701 ttagtttgat agatttttat tacactatct attataatat actaataaag attatcaaat
     2761 tcatctctcg aaaggagact tgcctgatgc ctactgaaga tacaacgatg tttgatcaag
     2821 tagcagaagt tattgaacgt cttcgcccat ttttattacg tgatggtggc gactgctcat
     2881 tgattgacgt ggaagacggt attgttaaat tacaattaca tggtgcatgt ggtacatgcc
     2941 caagttctac aatcactctt aaagctggta ttgagcgtgc attacacgaa gaagtgcctg
     3001 gtgtaataga agtagaacaa gtattctaat ataaagatat taagttaaca gaacatatta
     3061 tgaataacaa caaagacagg aatactgaat tatttataat cagttattac ctgtcttttt
     3121 tcgctgtcaa attggactga tgggaaagtt attttatact atttatctga ttaattattt
     3181 tattatagcc acatatccaa gcgcatatta ctattctatt ctatgaaatt taaaatataa
     3241 aaatagcaca tacgctcaat caaaatcgtt gagttatgtg ctatttgtat tttaattttt
     3301 aggtttatct acttcaggtt gtggttcacc tttcatatgt ttcgcaattt gctcatccat
     3361 ataaacccaa cctttccaac cgatgtgtac ggcatcactg ataacatatt tttcataatc
     3421 tttatcagtc atatcgtaaa ttttaccacc attatctaca acagtagaat ggattttttt
     3481 atacactgct tgacgacgtt ctttgtcgat gccaatgtgg tcataccata caccgtttga
     3541 tggaatactt acatattgaa catctgcacc agcagcacgc attgttttta caagtaattc
     3601 taaatcttgg aattctggag aattaacatt gaattcatag tcacgtctaa ctttacgctt
     3661 actttcttga attaatttcc agtattgatc tctaatacca aatttattcg atgtagtatc
     3721 agctttacca atttcaactg ctttttgttt catctcatcc caagaagcgt ctggttttgt
     3781 agcaggttta acatgttcta atggagattt atccattgca aacaatgatt taatcgcttc
     3841 tattttaatc aattgattct ctttaaaacc agaaatataa ctatctttag tttctttagg
     3901 gtttttagca taagatttca agtattcttt attgtgtaca tgtggaaact gtaataaacg
     3961 ctgtgcataa cgacgtttta attcagtaga catgtttttc tgctggaaca tttgattaat
     4021 ttgagtttga gacatacgag catcaaagtt ttgattcgtt aaaccatggt ttgtaaacca
     4081 ttgtggtgaa ataataaatg ttaatttctt accttttaat ttatcatact gtgatgcaag
     4141 ttcaactgcg ttaattaagt ctgtagaacc accagcacct aataagaatg cttttttgtt
     4201 ggcgttatgc ttatttaatg caattgcagg attaaatggg tcatctttac ctaattcact
     4261 agagccgtat ataggataat acttgtttga ttcgtataac ttatcttgaa tgagtgtgcc
     4321 ttttagtact tgatctgtca atgaagttct attatcttct acagtctttt cagttactaa
     4381 gcctgtaaac caacgtgctg gtaataatag aaagacaatg aataccgctc cactaattaa
     4441 aatgggtaaa aaaggtttta atttcatcgt aactcttcta atgcttcaac gattttattt
     4501 ggtgttgccc actcatctct atcaaagtcc ataatagata cttcgatatc aagtttattt
     4561 tgaatctcta ataataatcc aactgtttgg aaagaatcaa taataccttc ttcaaaaatt
     4621 tctacgtctg gattttcttt tacaatatca ttttctgcta cttctgctaa taaatttaat
     4681 acttgttctc taaattccat aattaaattc tcctttatta tataagttta cctgagaaga
     4741 ttaaaaagcc aaatgttaca aagtggaatg taatcacaat gctaagtgct gttgtgaaac
     4801 cattttgcca acgtggcgga tgtttcttgc gccaacgttc ataatagcca taacctataa
     4861 acaatgctgc atggtataaa ccataaacaa tgtaatacac ttcgatacca tgccaaattc
     4921 ccattatgaa gaagttgatt aagaatgcca cgttagacat tgcaaattga ctcttcaata
     4981 attttttacg agacatgtag aataaagatc tcatataaat acaatctctg aaccagaatg
     5041 ataatgtcat atgccatcta ttccagaaat ctttaatatt tttcgctttg aaaggtttat
     5101 cgaagtttgg tggtgtttta ataccgaata aataactaaa tgctatcgca aataaactat
     5161 aacctgcaaa gtcaaagaat aaatataagc tgtatgcgta catatataac cacaaattga
     5221 caaagccatg taggtctaat tgtaacggca tgattgcgta tgtgttaata aagtaagcaa
     5281 caatatattt atacaagaaa ccaagcataa tcatgtgaat tgcttttaat actaattcac
     5341 gatattcatt gcctgttggt acttttttat cgtctttaac gaaacgtttg taacgatcga
     5401 ttggtccaga tgaaatcgtt gggaagaatg aaataaattg aattaatttc catactttaa
     5461 tttctttgat agaaccatca cgaatttcca taattaactg cacacttttg aatgtaacat
     5521 aagaaattcc taagaaacca acaaattcaa ttaatttact ttcatggaag tgaatctgat
     5581 gtccacctaa ccatgtactt tgtaacactt tcacaagtgc taatggcaat attgataaaa
     5641 ccattacagt tacaaatttt gaaaatgaat tattttttgg ttttgaatga taataaaaca
     5701 ttatcaataa aacttgccat acgacgtaaa taataaaact aattaattga acacttaaat
     5761 acttttggtc aaacagatta tgtttatcag aagaaaagat taacacaatc ataaatgctg
     5821 taactacgcc attataaatg taacttcgct tacctaaaaa tccaagtata atgactggta
     5881 ataatgcaat taaagcaatt aagaagaatg taaaatcacc atatggaatc atccgttaat
     5941 tacctctgca attttctttc tgtcaatttt accatttgaa gtcaatggca attgttccat
     6001 ccattcaaac tttctaggaa tcatatactc tggtaagcgt gatttcaagt catttttaat
     6061 atttttagtc atttctgcat tgtccgtaac ttcagtcgtt ggcacaattg caccaattaa
     6121 atgaataact ttatcatttt tatatacagg tacaacaatc gcttctttta cgaactcaga
     6181 ctggcgtaat tgtgtttcaa tttcttctaa ttccattctg tagccattca atttgatttg
     6241 gaaatcaata cgaccttgaa tgaaccattg accattttca aacttcgctt tatcaccagt
     6301 gtgataagta cgaataccgt catcgaaatt aaatacttca gcagtttttt ggtcattttt
     6361 taagtatcct aaacttacac tttgaccttc gataacaagt tcaccttcat ctgtcgtaga
     6421 taatcttgcg cctggtcttt caacgccaac aggtaatgtc ggatattgat ctaagatttc
     6481 ttgtgtaatt tgaatacttg taactgctac cgtagcttca gttggaccat atgtgttgta
     6541 aatcgtcgca cttgggaaac ggcttactaa cgcttttgct gctctgtgag gtagaatttc
     6601 accacagaag aagaattcgt taagactacc atactgttct tcattaagcg ttggtaataa
     6661 taaacacatt tccataaatg atggtgttga tacccaaatg ttaatcggtg ttgctgttag
     6721 catttcattt aataatttag gtttattaat catgttttta tctacaagat ttaatgtacc
     6781 gcctgacgct aaacatggat aaatagccat tacagataaa tcaaatgaaa atggcgcttg
     6841 gttaagccat tgttgttcat ttcctgattt attaagttct aacatccact cagtaaattg
     6901 aactaaactt gcatattcaa tttgaacacc tttaggttcc ccagtagaac cagatgtaaa
     6961 gattgtgtat actgtgtcgt tatctttaat ctgactatca aaaattactg ggtcttgaga
     7021 tgttttaata tcttctattg taaatacttc acctgctaaa ctttcaaaca attcatcagt
     7081 cgtattaaat acaaactctg gttgaacctt gttaataatc attttaatac ggtcttcagg
     7141 aattgaagtg tctacaggta catatccaca tcctgcttta atggcaccaa tcatcccaac
     7201 aatcatatat ggtgacatgt gaccgaataa aatcatcggt ttcttactac cttgtaatcg
     7261 atgtgctaat ttactagact catccattaa ctgttgataa gttaattcat cagttgtgtg
     7321 tctaacagca atgctttgtg gatttgcatc cgcaaacgct tgcagcttgt taataatatc
     7381 tgtcatatta agtctccctc attagaactc attataaatg aagttattgt gtgtgtcgcc
     7441 actgccataa attaaatata aagtaataaa tattgccaaa tacaatagtg ttaataaata
     7501 tggtttgaat gcttcaacat atttattagg tggctgttta cttttagatt tcatattgca
     7561 cctcttaaag ttcttagtaa aaacgccttt ataaagaccg ttcaatataa aatacgtttt
     7621 aaaatttgtt ttttacaatt cattatatcg atattcataa tgaaattcaa ttttaatttt
     7681 atagattcaa catagtaatt ggttgtcatc actcaattat tgtaaatgat acactatgtt
     7741 gagaacatca ttcattttaa aggttattta aacaataaac aattacagtc tatataacaa
     7801 ttttgttata tacgtcaaaa tcaaataaac tcatcacatt aatatgacga gtttataatg
     7861 ttatggaatt aacatcagcg taattatata tattcgcaag tcaagcataa gatatttaac
     7921 aattgctttg cttgttttac caatgattaa aaaccatact tactttcaat ttactggagt
     7981 atgtggtacc tgatttgtca taaccgcttc tatattatta atacataatt gaatcatatt
     8041 gtcccgtgtt gttactgatg cgctgccaat gtgtggtgta atcagtacat tatcacgtcc
     8101 cattaatgga tgtgtatgat caattggttc atttgctaat acatctaaac cacatgctaa
     8161 tatttcttta ttgtctagtg catcgattaa tgctgtttca tctacaattt gtcctctacc
     8221 gatattaata aaaattgcat catttttcat ttgctcaaat gcttcagcat taaatttatg
     8281 atgtgtttct tttgtaagtg gcgctgtaca gatgataaaa tcactttctg ctaacaacgt
     8341 ttcaaaagaa acatatgttg cattaaagtc cgcctctgca tctttatgtc ttgatcgatt
     8401 atgataaaga attttagtat taaacccttg caaccttctt gcaaaagctt taccaatatc
     8461 tcccatacca tatattccaa tagttgaatt gaagacatct ttacctgaca ataaataagg
     8521 accccagctt tgccatgcat ctgcttctac atatttttca gcttctacaa tacgacgtgc
     8581 tgtagcaagc attaatgtaa atcctaattc tgcagttgtt tcagtaagaa catttggtgt
     8641 gttcgtgaca accacgttgt tcgctgttgc actttcaaca tcgatgttgt catatcctac
     8701 agccatattc gcaattactt ttaaatttgg tgattgtgct aaaatttctg catcgatttg
     8761 ttcacttaat gttataaaac aagcatctgc gtcacgaagt gctgtcacaa attgatcttt
     8821 aggcataggc actaatgatt tttgccacat aacaacgtca ccaagtttac tcaattgttg
     8881 ataaaattta tctggaattt tcctcgaaac aactattttt accaaatcta ttcacctact
     8941 ttataaaatg aatatatact atttttctaa ttcagctatt gcttcattta aatctttaaa
     9001 agaatacgtt ggtggcacat ttttattttg cacatcttct aacgtagata cacctgtttg
     9061 tacatgaatc gtatccatac ctacgttaat accagacata atatcggtat cgtacaaatc
     9121 gcctaccata gcaacttcgg atttatctaa tcctaaaatt tctaatgctt taaccataat
     9181 aatcggttct ggtttaccaa taaattgtgg cgatacacct gtcgatacac ttacaacact
     9241 tgtaatagca ccattccctg gtaaaaatcc tcgctctttt ggaattgaca catctggatt
     9301 tgtagaaata aatgttgctc catttcttac acctaacgtt gcaatcgcaa gcttttcata
     9361 tgtaacttgt tcgtcaagtc caataactac ataatcaaca tgctcgtcat ttttaatgac
     9421 aagtcccgct tcggttaacg cagtatttaa accactccca cctaacatat atactgatgc
     9481 tcctggtgat tgttctgaaa tataatcagc agtggctaac gctgacgtta caacctcttc
     9541 tggtttagca tcaatgtgca tttcacgtaa tttttcagtt acttgctcag gtgttttcgt
     9601 tgaattatta gttacgtata aatgaggaat gccttttaca tttaaataat cgatgaattg
     9661 ttttgctcca tcaatctcat ctgttcccat atacattgtg ccatctaaat cgattaaata
     9721 cgctttatac tgtttcatta ttgattttct cctttaccaa aagctgttac tggtacattt
     9781 tcttgatgta aaaatgtcgt tacttctgta ataaattgtt tgtaataagg taatgcgtca
     9841 tcaaacattg gcatgataac cttaatatct aaggctgtgt aatcatatgt aaattgttta
     9901 cgaatatcga cagttttatt gattgcctgc tgtgtttctt tagtaataac attttctagt
     9961 tctaatatat caatcacatc tttataatta ccaggatccc ttaaaataaa tgcatcgata
    10021 atcatattcc ctatatctac cgatgattct atcaacattt gagcaatgcg ttcaaatgca
    10081 taatgattgt gcttgctctc atgataatca tcagttaatg cttgtaaata ggctaatttc
    10141 tgagttagtt tatctttgtc tacaaaatac attccttgtc acctctcgct tatatattat
    10201 cataattttg cagtatactt catttgtcgt tatactaact ttactacttt taaaaggagt
    10261 cgctacaaaa tgattgatat gtatttatat gatgacaacg aagagagtca agttcagttt
    10321 gttggttttg ttggcgagca tagtcgatat gatttgatgt tagtccatac aaatagacat
    10381 tatggtaaga cactcgtact taatatgcaa acaaataaat tcggtattat cggtactgat
    10441 gatttaaaag aagaaggcta tatcgctcat attttaggtg taaatgctga agaaggcgat
    10501 gaaattactg agtatttaaa tgaagtcatt cattaaagat aaaaacccct aactagatta
    10561 caggtccatg cttgaattgc atgattccca ttttcaatct agctaagggt tttattaatg
    10621 ttcagtatct tgaatggtcg gcttaatctt cacatcttga agtttattgt cgtcatctat
    10681 cacatcagac ggatgaactt cttcgattaa ctttttaacc tcttgtttag acaaatgtct
    10741 taatacaaag taaggacaac caaagttaca atattcaaat atgtaatctt gaatatttga
    10801 aaaacgttta ctcatctctg cttttttatt agaatctttg taaaagcctt ttaatcgtaa
    10861 ttgatcgtaa ccatagtcac caactatgta atcatattta tctaaaatat ctgaatacct
    10921 agcaataaat tgttcttcat taaaacattc gcgataattt tctattaatt caaagtaatg
    10981 ttgatctact tttatcaaat tcttcaccta tctatgacaa tgaaaggctc agtttatact
    11041 tttagtgttt cataaaaata atgattactt ctatgacacg ttcgttttgt acaaactaag
    11101 cctttatgcc ttattaatgg ttaaatatta actatttaac tgtgcctcac cttggcgttg
    11161 cttttcttta gcagcttcat ttacttgctc atccgcatga tatgaactac gtactaaagg
    11221 tccagcttgg caatgtttaa accctttatc cattgccact tttcttaatt taccaaactc
    11281 taaaggcgtg taatattttt gaacctttaa atgtttacgt gaaggttgta aatattgacc
    11341 aatcgttaaa atatctacat cattcgcacg taaatcatcc atcgtttcat aaatttcttc
    11401 tatagtttca cctaatccaa ccataatact tgatttagtt gggatatccg gttgtaattc
    11461 ttttgaacga cgtaaaaact ctaatgttct gtcgtatgtc gcacgcgcac gaactctcgg
    11521 tgttaatcga cgaacagttt caatattatg gtttaaaatg tcaggtcttg aagccattaa
    11581 tgtttctaac gcatcatagt ccccgcccat atctgatggt aaaatttcaa tcgttgtaaa
    11641 tggatttctt tctctaactt tacgtactgt ctcagcataa acatttgaac cagcatctct
    11701 taaatcatca cgtgcaacag cagtgataac aacgtgtttc aaattcatta attcaactga
    11761 ttcagctaca cgttcaggct catttaaatc aagttcatta ggtaaacctg tcttaaccgc
    11821 acaaaaacga caagctcttg tacatacggc acctaaaatc ataaatgtcg ctgtacgacg
    11881 tgcaccccaa cattcatgta tattaggaca tttagcttct tcacatacag tattaagatt
    11941 tttttccctc atcatcttct taagtcctgt atagttttcg ttggtattta attttatttt
    12001 caaccaatcc ggtttacgta atatttcctc gttttttgtc gccataacaa cacataccct
    12061 cctgttaagt gtatctttaa acattatatc gaaattcatg acaaatgaaa atccaataat
    12121 cttacttttt aatattccaa taatttttct ttaaatatat ctcttaaaaa ctctggcatt
    12181 aaatactctt ttggtaattc tttcaatgtt gggaaataac gaccgaacgt atacatatct
    12241 atcgtaccta atac
//
LOCUS       DAPIDM010000074        12073 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000074 DAPIDM010000000
VERSION     DAPIDM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.074"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..116
                     /locus_tag="RJE42_001712"
     CDS             <1..116
                     /locus_tag="RJE42_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin transporter RibU"
                     /protein_id="HDV5877128.1"
                     /translation="KVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLKRI"
     gene            complement(222..470)
                     /locus_tag="RJE42_001713"
     CDS             complement(222..470)
                     /locus_tag="RJE42_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDV5877129.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            578..1531
                     /locus_tag="RJE42_001714"
     CDS             578..1531
                     /locus_tag="RJE42_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5877130.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            1521..2900
                     /locus_tag="RJE42_001715"
     CDS             1521..2900
                     /locus_tag="RJE42_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV5877131.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            3053..4507
                     /gene="ebpS"
                     /locus_tag="RJE42_001716"
     CDS             3053..4507
                     /gene="ebpS"
                     /locus_tag="RJE42_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDV5877132.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQHHDESQASDDNAHNEAGTLDDRQVESSAHSSVN
                     QEPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKDAENNTEHSTVSNKSK
                     AEQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDESKDHHSGKKGAAIGAGTAGVAG
                     AMADSKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLAGA
                     AASKSASAASKPHASNNASQNHDEHDHHDRDKERKKGGMAKVLLPLIAAVLIIGALAI
                     FGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQDKA
                     TKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRIAI
                     QYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            4633..4749
                     /locus_tag="RJE42_001717"
     CDS             4633..4749
                     /locus_tag="RJE42_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877133.1"
                     /translation="MLFNKVLSQILTYQLNFLNSHQKDKTPQSTNLTRKNKK"
     gene            4910..5896
                     /locus_tag="RJE42_001718"
     CDS             4910..5896
                     /locus_tag="RJE42_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDV5877134.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSIGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(6011..6979)
                     /locus_tag="RJE42_001719"
     CDS             complement(6011..6979)
                     /locus_tag="RJE42_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV5877135.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISLHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNEKLNVPLVKAYMGLPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            7056..7715
                     /locus_tag="RJE42_001720"
     CDS             7056..7715
                     /locus_tag="RJE42_001720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV5877136.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSKI
                     K"
     gene            7746..7862
                     /locus_tag="RJE42_001721"
     CDS             7746..7862
                     /locus_tag="RJE42_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877137.1"
                     /translation="MQRLTHIQNECADNDLLNLKGDFYSMMNESCLFIFGKS"
     gene            7979..8170
                     /locus_tag="RJE42_001722"
     CDS             7979..8170
                     /locus_tag="RJE42_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877138.1"
                     /translation="MSVKNLSIAKAADLVTKNDLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            8427..9602
                     /gene="rpsA"
                     /locus_tag="RJE42_001723"
     CDS             8427..9602
                     /gene="rpsA"
                     /locus_tag="RJE42_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV5877139.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     RENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            9824..11134
                     /gene="der"
                     /locus_tag="RJE42_001724"
     CDS             9824..11134
                     /gene="der"
                     /locus_tag="RJE42_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV5877140.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            11151..>12073
                     /locus_tag="RJE42_001725"
     CDS             11151..>12073
                     /locus_tag="RJE42_001725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV5877141.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKR
                     ISKLTQDLFMNDYLRVYTNDDLVGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNAL"
BASE COUNT         4477 a         1780 c         2175 g         3641 t
ORIGIN      
        1 ttaaagtaat cattgtttca ggaattatac cattcaatat tattaaaggt atcgtcattt
       61 caattgtatt tatattacta tatagaaggc ttgcgaattt cttgaaaaga atttaatcaa
      121 attaaagcaa aataatatac acataataat aaaaagcagg tgactatcaa taaacgatag
      181 cttgcctgct ttttctatgg aacatttgtc taaaaaatca attattcaaa ttttaaagcg
      241 tccccatcaa atgattcgtc tgcaatttta atagaatctg tagggcatcc atcaattgca
      301 tcttccatat cttcatataa ttcctcaggt acttctgcag taccttggtt atcgtcaagg
      361 attacgaaag caataccttc gtcgtcgtaa tcatatatat ctggcgctgc tgcaccgcat
      421 gcaccacatg caatacaagt atccatatca acgattgtat attttgccaa tgtcttcgcc
      481 tcctttgata aaaatgctaa aatagtaatg tgactaaaat tttagacagc atcattttta
      541 ttttcaaatt atccgtttta cagagtgagg gttaaatttg caacacatta taaaaacagc
      601 attacaacaa acatttaact ataaaacaaa taaaagtatt tacaatatct tagttggtaa
      661 gaaatctcac caaacctttt ttgacgcttg tagtcaacaa cagttgtcat tatatcacag
      721 tttaccacta ttaaaatatc cgtcttttga gctattttta gaaaaaatca atgaatttaa
      781 tgctgaaatg gaaatcatgt tgcatcctag atatacattt gaaagcatgg ggcaaacatt
      841 tcaagcaatt caactattag tgcaaaccat gtctaatacc aaacaacatg tttttcattt
      901 tgtaccaatc tctcaaaata ataagataca agaaactgta aagattgtct ataactacat
      961 taaagaaaac caattacaaa ttgattttga aaatgaacta cataacttgt ttaaagcaat
     1021 aactttaaaa gggccatgtt acttacatta ttatttgcaa ggctatgatg aaccaatgta
     1081 tacgagacag caagttagtt taatagaaaa gctatctcaa cagcaattgt ttgaatacga
     1141 aatgaataat ttagtgacaa tgatgtttga attagaaagt ggagaatata ctattttatc
     1201 aaaaataata atgaaaccta cattattaaa tcaaacttat attacttata caaaattgct
     1261 tgaacaattc acgatggaag atatagcggc tcaacaacaa gttaaaatca atactatcga
     1321 agatcatgta cttgaaatct taatcaaagg ttacatgtct aattacgatg attatgttga
     1381 actagaagat caactccagt ttttgaattt ttatcaacaa catcgtggcg aacgattaaa
     1441 attttacaaa gaacaatttg acacgttatc atattttcaa ttaaaagtat taatcgttgg
     1501 atttgaaaga ggtgatctga atgttgcatg atattttacg aaacaaattt ggcttcgaga
     1561 attttaaacc gggacaacag gaaattatag aaagtataat gtctcaacag cacactctag
     1621 gtattcttcc aactggaagt ggaaagagtt tgtgttatca aatacctacg tatttatcag
     1681 gtaagccgac attaattatc tcaccgttaa tatctttaat ggatgaccaa gttatgcagt
     1741 tgaaaataaa tggagaaaaa cgtgtaacat gtattcactc tggtatggat gaaattgaga
     1801 aaaagcataa tattaaatgt ttacgacata gccgtttcat ctttctaagt ccagaatttc
     1861 tcctgcaacc gtcaaatttt aaattaatat ctatgataga ctttggcatg attgttctag
     1921 atgaagcaca ttgcctatct gaatggggat atgatttcag accacattat gctctaatag
     1981 gaaaagtaac aaagcatttt aaagaagcgg ttgtcttagc attgacagca actgcaccac
     2041 cgcatttaca agatgatttg acggaaatgt tagcgattca attcaatgtt attaaaacta
     2101 caatgaatcg cccaaacata agctttaagc atcttaattt tcatgatgat gaagataaaa
     2161 ttgaatggtt gctgccgttt ctacaacagt cgggaccaac gattatttat gtctcatcga
     2221 aaaagatgtg tctgaattta gcgcaactta tttatgattc aggttttctt acaggtattt
     2281 atcatggtga tatgaattat caagagcgac acacagttca acaacaattt ttaaataatg
     2341 atattccgat tatagtcgca acgagtgctt ttggtatggg aattaataaa aaagatattc
     2401 gcacaatcat tcactttcat ctttcaacaa gtccttctaa ctacattcaa gaaattggcc
     2461 gtgcgggtcg cgatggtgaa ctaagtcagg caattagttt atttcaaccg gacgataaat
     2521 atattttaga aacgttatta tttgcagata tgataacaga agaagatgta caaaatttcg
     2581 aaataggaga atttttagca cccgataaac aagacgtttt gacaacgttg cattcattct
     2641 atagtatcgg cgccttgaaa cagatattta agcaatcatt taaacgaaag caattaggat
     2701 tctttcgcat gattggctat tgcaaattag atcaatgtag acggaagtat ttattagaat
     2761 ttttcggtga atatccaccg gcacaagatc gatgttgtga caatgattct aatataactg
     2821 atatcgtaat tttaaataag aagaaggtaa ttagaagtat tggatttgat gaaaagttgc
     2881 aaaatttatt tctcagatag tattacttta ctaaaagaaa attgacaagc tataattagt
     2941 gtatacacaa ttgaaaaatg attgaaataa ttttgaaaaa tatacataaa catatgtcat
     3001 gtgggtatat tttatgtaaa atcattgtaa tagaatagaa aggaagatgg ctatgtctaa
     3061 taattttaaa gatgactttg aaaaaaatcg tcaatcgata gacacaaatt cacatcaaga
     3121 ccatacggaa gatgttgaaa aagaccaatc agaattagaa catcaggata caatagagaa
     3181 tacggagcaa cagtttccgc caagaaatgc ccaaagaaga aaaagacgcc gtgatttagc
     3241 aacaaatcat aacaaacaac atcacgatga atcacaagca tctgacgaca atgctcacaa
     3301 tgaggctggc acattagatg atcgtcaagt cgaatcatca gcgcatagtt ctgtaaatca
     3361 agaacctagc catcaagaca gtacacctca acatgaagag gaatattata ataagaatgc
     3421 ttttgcaatg gataaatcac atccagaacc aatcgaagac aatgataaac acgagactat
     3481 taaagatgca gaaaataaca ctgagcattc aacagtttct aataagagta aagctgaaca
     3541 atctcagcaa cctaaaccat attttacaac aggtgctaac caatcagaaa catcaaaaaa
     3601 tgaacatgat aatgattctg taaaacaaga tcaagatgaa tctaaagatc atcatagtgg
     3661 taaaaaaggc gcagcaattg gtgctggaac agcgggtgtt gcaggtgcaa tggctgattc
     3721 taaagctaag aaacattcaa atgacgctca aaacaaaagt aattctggca aggcgaataa
     3781 ctcgactgag gataaagcgt ctcaagataa gtctaaagat catcataatg gcaaaaaagg
     3841 tgcagcgatc ggtgctggaa cagcaggttt ggctggagcc gcagcaagta aaagtgcttc
     3901 tgccgcttca aaaccacatg cctctaataa tgcaagccaa aaccatgatg aacatgacca
     3961 tcatgacaga gataaagaac gtaaaaaagg tggcatggcc aaagtattgt taccattaat
     4021 tgcagctgta ctaattatcg gtgcattagc gatatttgga ggcatggcat taaacaatca
     4081 taataatggt acaaaagaaa ataaaatcgc gaatacaaat aaaaataatg ctgatgaaag
     4141 taaagacaaa gacacatcta aagacgcttc taaagataaa tcaaaatcta cagacagtga
     4201 taaatcaaaa gatgatcaag acaaagcgac taaagatgaa tctgataatg atcaaaacaa
     4261 cgctaatcaa gcgaacaatc aagcacaaaa taatcaaaat caacaacaag ctaatcaaaa
     4321 tcaacaacag caacaacaac gtcaaggtgg tggccaaaga catacagtga atggtcaaga
     4381 aaacttatac cgtatcgcaa ttcaatacta cggttcaggt tcaccggaaa atgttgaaaa
     4441 aattagacgt gccaatggtt taagtggtaa caatattaga aacggtcaac aaatcgttat
     4501 tccataatat aactatataa attgtaactg aactgctata tacaaacgtg catattataa
     4561 agtatctcta aatattttag agaacttaaa ttgcgcttat aaggatgtag cagttttttc
     4621 atttttcaaa gtttgttatt taacaaggtg ttgtctcaaa tattgacata tcaattaaac
     4681 tttttaaata gtcatcaaaa agataaaaca ccacaatcaa caaatttaac gaggaagaat
     4741 aaaaaataaa tcaacatatt aaattgtagt gctattcaac tccgtagcta acaattctct
     4801 atgcacatta aacaaattgt caaaaatata tcataaatct tcaagcacag acttagcgca
     4861 tcaatcactg aactgttata atagtttggg attaaaggag gccgaaacaa tgcaaaaagt
     4921 tgaaagtatc ataattggtg gagggccatg cggattaagt gcggctattg aacaaaaaag
     4981 aaaaggtatt gataccttaa ttattgaaaa gggtaatgtc gttgaatcaa tctacaatta
     5041 tcctactcac caaacatttt tctcatcaag tgataaatta agtattgggg acgtaccgtt
     5101 tatcgttgaa gaaagtaaac caagacgtaa tcaagcgcta gtttattacc gagaagttgt
     5161 aaaacatcat caattaaaag taaatgcatt tgaagaagta ttaactgtta aaaaaatgaa
     5221 taataaattt acaattacta cgacgaaaga tgtttatgaa tgtcgatttt taacaatcgc
     5281 gacaggctat tatggtcagc ataatacatt agaagttgaa ggtgcggatt tacctaaagt
     5341 gttccattat tttaaagagg cacatccgta ttttgatcaa gatgttgtta ttattggtgg
     5401 taagaattcg gctatcgatg ctgctttgga gttggaaaaa gctggtgcta acgtgacggt
     5461 tctatatcgt ggtggagatt attcgccttc aattaaaccg tggatacttc caaatttcac
     5521 agcattagta aatcatgaaa aaattgacat ggaatttaat gctaatgtta cccaaataac
     5581 tgaagatact gtgacttatg aagtaaatgg tgaaagtaaa acgatacaca atgattatgt
     5641 ttttgcgatg attggttatc atcccgatta tgaattttta aaatctatag gcattcaaat
     5701 taatacaaat gaatttggaa cagcgcctat gtataataaa gaaacatacg aaacaaatat
     5761 cgaaaattgc tacattgcag gtgtgattgc tgcagggaac gatgcgaata ccatttttat
     5821 tgagaatggt aaattccacg gtggcattat tgctcaaagc atgctagcta agaaacaaac
     5881 gccgttagaa tcataaaaat aaaggtctat gtactaaata acttagtttt acaacgactg
     5941 acattcatga tatgtcaaat gaggttgatg actaattatt gtacatagac ctttttatgt
     6001 taagtaatca ttataattca aaatatgatt tgatttcagc tttatctaaa ttgttgctta
     6061 acgcaactaa taattttaat cttgcttttg gaccattcaa gccgttagaa aaaataaaac
     6121 cttgttgtgc aagttggtaa ccaccaccat cgtatgcgta agttggactc acaataccat
     6181 taaaggaacg tgaaactagc acaataggta tatttaaaga tactaattgt tgaatgcctt
     6241 ctaatgcgct tggaggtatg ttgccttgcc ctaacgcttc aataaccata ccatcgatac
     6301 cttcacgact ataaaaacta aaaatgtcac ctggcaaacc catatatgct tttactaatg
     6361 gaacatttag tttctcattg acattttcta atgcttgttg gcgatatggc atatgatgga
     6421 attgcacacg atcctttgtc aatacaccta gcggaccatg atttggactt tgaaatgtgt
     6481 ttgtattaga cgtatgtgtt ttggtaacat tacgcgccgt atgtatttca tcattaaata
     6541 caaccatcac gcctttatga cgggcctttt catcagaggc aacgcgaata gcggaaatat
     6601 aattatataa tccgtcagaa ccaatttcat tagacgagcg cattgcgcca gtaataacaa
     6661 caggttgctc gatacctaat attaaatcaa gtaaaaaggc agtttcttct aacgtatcgg
     6721 taccatgcgt gataacgaaa ccatcataat atttatttgt tactgcttct aaaataatat
     6781 cctttaattg tttaacatgt tggattgtca tatgaggtga tggtacatta aaaggattta
     6841 attcatctat ttgtgcatat tgatttatga catcttgatg cagtgaaata gggttaatat
     6901 catttgttac tactttatta gattggtctt gtgacatact aatggtgcca ccagtatgaa
     6961 taacaagtag atgtttcata tatttcctcc tatatttcat ttacttaatt atgataaaat
     7021 attattcata aaacgacaag gaagggaaat gacgcatgaa agccattaat attgcattag
     7081 atggtccagc tgctgccgga aaaagtacaa ttgcgaaacg tgtagccagt gaactatcaa
     7141 tgatttatgt agatacagga gcaatgtatc gtgcattaac atacaaatat ttaaaattaa
     7201 acaaaactga ggactttgca aaactagttg accaaacaac attagattta acttataaag
     7261 cagataaagg tcaatgtgtc attttagata acgaagatgt aacagacttt ttaagaaata
     7321 atgatgtgac gcaacatgtt tcatacgttg catctaaaga gccagtacgt tcattcgccg
     7381 ttaaaaaaca aaaagagtta gctgcagaaa aaggtatcgt aatggatggt cgcgatatcg
     7441 gaactgtagt gctaccagat gcagatttaa aagtatatat gattgcatca gttgaagagc
     7501 gagcagaaag aagatataaa gataatcaat taagaggtat cgaatcaaat tttgaagatt
     7561 taaaacgtga tattgaagca cgtgatcaat acgacatgaa ccgtgaaata tcaccattaa
     7621 gaaaagcaga tgatgcagtg acattagata cgacaggcaa gtcgattgaa gaagttactg
     7681 acgaaatttt agcgatggtg agtaaaatta aataatctaa taagtcataa ctatctattt
     7741 gtaatgtgca acgcttaacg catatacaaa atgaatgtgc tgataatgat ttactcaact
     7801 taaaaggtga tttttattca atgatgaatg aaagttgcct ttttattttt ggtaaaagtt
     7861 aatgcgtcag tgaattgtgt aagtttttca aaaagtaaaa agaaataata aaggtgaatt
     7921 attagaattc caaaaataat tcattacatt cataaagctt tttacaaatg gtaagaaaat
     7981 gagtgttaaa aatctaagta ttgcaaaagc agctgattta gtcacaaaaa atgacctatg
     8041 taataattcg agaaagatgc actatatacg gtcttcttac tattcaaatg taaaagttgc
     8101 ttatttgcgt ggctttttgt tttataaaag tataaaattt tactataata tatcttgtag
     8161 agaacaatga aatgacagac ttataataca aaatagtaag ctatgctaat ttttatacaa
     8221 aaaatatgta taaacatagc ggactttatt acttgtttga atatatggtt gtaataaata
     8281 tatgtccaac taatacacag aatgagatta agtgacatat ttatggatgc aactaattta
     8341 ctaaatgtaa taaaggtaga atttcttgac aattctgtca gtttataaga tgttataaat
     8401 atgtagtgta taaggaggca aacaagatga ctgaagaatt caatgaatca atgattaacg
     8461 atattaaaga aggtgacaaa gtcactggcg aggtacaaca agttgaagac aagcaagttg
     8521 ttgttcatat caacggtggt aaatttaatg ggattattcc tattagtcaa ctatctacgc
     8581 atcatattga tagcccaagt gaagttgtaa aagagggcga cgaagttgaa gcatatgtca
     8641 ctaaagttga gtttgatgaa gaaaatgaaa ctggagctta catcttatct agaagacaac
     8701 ttgaaactga gaagtcttat agttatttac aagaaaaatt agataataat gaaatcatcg
     8761 aagcgaaagt aacagaagta gttaaaggtg gtttggttgt tgatgtagga caaagaggtt
     8821 ttgttccggc ttcactaatt tcaacagact tcattgagga tttctctgtg tttgatggac
     8881 aaacaattcg tattaaagtt gaagaattgg atcctgaaaa taatagagtc attttaagcc
     8941 gtaaagcagt tgaacaagaa gaaaacgatg ctaaaaaaga tcaattatta caatctttaa
     9001 atgaaggcga tgttattgat ggtaaagtag cgcgtttaac tcaatttggt gcatttatag
     9061 acattggcgg tgttgatggt ttagtgcatg tatctgaact ttctcacgaa catgttcaaa
     9121 caccagaaga agttgtttca attggtcaag atgttaaagt taaaattaaa tctattgata
     9181 gagatacaga acgtatttca ttatcaatca aagatacgtt accaacacct ttcgaaaata
     9241 ttaaaggtca attccgcgaa aatgatgtca ttgaaggtgt cgtagtaaga ttggcaaact
     9301 ttggtgcatt tgttgaaatt gcaccaggtg tacaaggact tgtacatatt tctgaaattg
     9361 cacacaaaca cattggtacg ccaggtgaag tattagaacc tggtcaacaa gtaaatgtta
     9421 aaatattagg tattgatgaa gagaatgaaa gagtatcact atctattaaa gcaacattac
     9481 caaacgaaga tgttgttgaa agtgatcctt ctacgactaa ggcgtactta gaaagcgaag
     9541 aagaagataa tccaacaatt ggcgatatga ttggtgataa acttaaaaat cttaaactat
     9601 aatttaatat ttaatagtaa actccacatg tttatgattg catgtggagt atttttatgt
     9661 aacaaaatat attcggaatg ataacgtgga caaatttaac taagtgtttt ataagataaa
     9721 gttttaagtg ctgattttta tcattacagt aataaactca ttttgaatac acagtctcat
     9781 gtgatattat taaaaagata taagaaagag aggaagttag cttatgacta aacctatagt
     9841 agctattgta ggtaggccta atgtaggtaa atctacaatt tttaatagaa tagttggaga
     9901 acgtgtttcg attgttgaag atacgccagg tgtaacacga gatcgtattt attcttcagg
     9961 tgagtggtta acacatgatt tcaatattat tgatacaggt ggtattgaaa ttggtgatgc
    10021 accattccaa acacaaatta gagcgcaggc agaaatcgcc atagatgaag cggatgttat
    10081 tatttttatg gttaacgtgc gtgaaggatt gacacaaagc gatgaaatgg tcgctcaaat
    10141 tttatacaaa tctaaaaaac cggtcgtatt agcggttaac aaagtagata atatggaaat
    10201 gcgtacagac gtgtatgatt tctattcatt aggatttggt gaaccgtatc cgatatcagg
    10261 gtcacatggt ttaggacttg gtgacttgtt agatgcagtt gtttctcatt ttggtgaaga
    10321 ggaagaagat ccttatgatg aagatacaat tcgactatcc attattggac gaccaaacgt
    10381 aggtaaatca agtttagtaa atgctatttt aggtgaagat cgcgttatcg tttctaatgt
    10441 tgcagggaca acgagagacg ctattgatac agagtatagt tatgatggac aagattatgt
    10501 tttaatcgat actgctggta tgcgtaaaaa aggaaaagta tatgaatcaa ctgagaaata
    10561 ttcagtatta agagctttaa aagcgattga acgttcaaat gttgttttag ttgtcattga
    10621 tgcagaacaa ggcattattg aacaagataa acgtgttgca ggatatgcac atgaacaagg
    10681 taaagcagtc gtgattgtcg taaataaatg ggatactgtg gaaaaagata gtaaaacgat
    10741 gaagaaattt gaagatgaag tacgtaaaga attccaattt ttagattatg cacaaattgc
    10801 ttttgtgtct gctaaagaac gcacaagatt acgtacatta ttcccttaca ttaatgaagc
    10861 aagtgaaaac cataaaaaac gtgttcaaag ttcaacttta aatgaagttg ttactgatgc
    10921 aatttccatg aaccctacac caacagacaa aggtagacgt ttgaatgtct tttatgcaac
    10981 acaagttgct atagaaccac cgacatttgt tgtatttgtt aatgatgtag aattaatgca
    11041 tttttcttat aaacgctatt tagagaatca aatccgtgcc gcttttggtt ttgaaggtac
    11101 accaattcat attatagctc gaaagagaaa ttaacgattg ggggataaca atgactaaaa
    11161 ttaccgtttt tggtatggga agttttggga cagcccttgc caatgttctt gcagaaaatg
    11221 gacatgatgt tttgatgtgg ggtaaaaatc aagatgctgt tgatgaatta aatacatgtc
    11281 atacaaataa aaagtattta aaatatgcga aattagatgt taacatcatc gctacatcag
    11341 atatgaccaa agcaattcaa tttgcagata tttacttaat ggctttacct actaaagcaa
    11401 tgcgagaagt tgcttctcaa attaatgata agctgacatc taaaaagact tttatacatg
    11461 ttgctaaagg tattgaaaat ggaacgttta aacgtgtgtc agaaatgatt gaagattcta
    11521 tttcacctga atataatgca ggtattggcg tgttgtccgg gccaagtcat gcggaagaag
    11581 ttgtagtaaa gcaaccaact acagttgctg catcatcaaa agataaaagg ataagtaaat
    11641 taacgcaaga tttatttatg aatgattatt tacgtgtgta cacgaatgat gacttggttg
    11701 gtgttgaact tggtggtgct ttgaaaaata tcattgcagt agcaagtggt atcgtagctg
    11761 gaattggcta cggtgataat gcaaaagcag cattaatgac tcgtggctta gcggaaatta
    11821 gcagattagg tgaaaagtta ggtgccgatc ctatgacatt tctaggttta ggtggtatcg
    11881 gtgacttaat cgttacttgc acatcaacac attctcgaaa tttcacatta ggatataaac
    11941 ttggacaagg tgaatcaatg gatcaagcat tatctgaaat gaatatggtt gttgaaggta
    12001 tttatacaac taaatctgtt tatcatttag ctaaagaaaa aaatgtggat atgccaatta
    12061 caaatgcatt ata
//
LOCUS       DAPIDM010000075        11948 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000075 DAPIDM010000000
VERSION     DAPIDM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.075"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            173..418
                     /locus_tag="RJE42_001726"
     CDS             173..418
                     /locus_tag="RJE42_001726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877142.1"
                     /translation="MQVGAGPNTEADEKSAYNNVQVGDGRQQRESGFPISTDNASWRG
                     PNTETGGKSVYNNVQVGVGRRNKFYENIISVPLPSKE"
     gene            708..3413
                     /gene="acnA"
                     /locus_tag="RJE42_001727"
     CDS             708..3413
                     /gene="acnA"
                     /locus_tag="RJE42_001727"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV5877143.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVLKLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDELGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3594..4061
                     /locus_tag="RJE42_001728"
     CDS             3594..4061
                     /locus_tag="RJE42_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV5877144.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQALNNEGKIVEIMDGIDSL"
     gene            complement(4268..4564)
                     /locus_tag="RJE42_001729"
     CDS             complement(4268..4564)
                     /locus_tag="RJE42_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDV5877145.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQHYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSKK"
     gene            complement(4942..5550)
                     /gene="plsY"
                     /locus_tag="RJE42_001730"
     CDS             complement(4942..5550)
                     /gene="plsY"
                     /locus_tag="RJE42_001730"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV5877146.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5756..7747
                     /gene="parE"
                     /locus_tag="RJE42_001731"
     CDS             5756..7747
                     /gene="parE"
                     /locus_tag="RJE42_001731"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV5877147.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISTEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPDTRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7747..10149
                     /gene="parC"
                     /locus_tag="RJE42_001732"
     CDS             7747..10149
                     /gene="parC"
                     /locus_tag="RJE42_001732"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV5877148.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVIINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITIITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10399..11859
                     /locus_tag="RJE42_001733"
     CDS             10399..11859
                     /locus_tag="RJE42_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDV5877149.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4165 a         1754 c         2292 g         3737 t
ORIGIN      
        1 attatacttt tatagataat taactcaggc tgggacataa atcaatattc tatgctctac
       61 gaagttatat tggtagtagt tgactgaacg aaaatgcgct tgtaacaagc ttttttcaat
      121 tctagtcagg ggccccaaca cagagaaatt ggctccccaa tttctacagg caatgcaagt
      181 tggggcgggc cccaacacag aagctgacga aaagtcagct tacaataatg tgcaagttgg
      241 ggatggacgc caacaaagag aaagtggatt cccaatttct acagacaatg caagttggcg
      301 gggccccaac acagaaactg gcggaaagtc tgtttacaat aatgtgcaag ttggggtggg
      361 acgacgaaat aaattttacg aaaatatcat ttctgtccca ctcccatcaa aagaatgaca
      421 ttgaaaagaa attttaaagt ttgacgccat aattttcttt ctgctcaatg aaataatacg
      481 tttaaaaatc gaattgaatc attcacgaaa ttgataatta ttctcaatta ataaaaaata
      541 cttattacac cttacctgca tgataagttt tacttatata ctctgattaa aaagtcaaaa
      601 cctaaattaa gcgttttcta agcatgatga gttgtttatc aataaatata ataatacaat
      661 ttaatcaagg catataaata taaaaatgta tcaaggggga tcattaaatg gctgcaaatt
      721 ttaaagagca atcaaaaaaa cattttgact tgaatggcca aagttatact tactatgatt
      781 taaaagctgt agaagagcaa ggtattacta aagttttgaa attaccttat tcaattcgtg
      841 ttttgttaga atctttactt cgtcaagaag atgattttgt aattacagac gatcatatta
      901 aagctttaag tcagtttgga aaagatggaa atgaaggcga ggtaccattt aaaccttctc
      961 gtgttatttt acaagatttc acaggtgtac cagccgtagt tgatttagct tctttacgta
     1021 aagcaatgga tgacgttggg ggagatatta ctaaaattaa tccagaagta ccggtggatt
     1081 tagttattga ccactcagtt caagtggata gctatgcaaa tccagaagca cttgaacgta
     1141 atatgaaatt agaatttgaa cgtaactatg aacgttatca gtttttaaat tgggcaacga
     1201 aagcatttga taattacaat gcagttcctc ctgcaactgg aatagttcac caagttaacc
     1261 tagaatattt agcaagtgtt gtacatgttc gtgatgtaga tggtgaaaaa actgcatttc
     1321 cagatacatt agttggtact gattcacata caacaatgat aaatggtatt ggcgtactag
     1381 gatggggtgt tggtggtatt gaagctgaag ctggaatgct tggacaacct tcttatttcc
     1441 caattccaga ggttattggt gtacgactag taaattcatt accacaaggc gcaacagcaa
     1501 ctgatttagc gttgagagta actcaagagc tacgtaaaaa aggtgttgtt ggcaaatttg
     1561 tagagttctt tggtccaggt gtacaacatt taccactagc agaccgtgct acaatcgcaa
     1621 acatggcacc tgagtatgga gcaacttgcg gattcttccc agttgatgat gaatctctta
     1681 aatatatgaa gttaactggt agatcagacg aacatatcgc gcttgtaaaa gaatatttga
     1741 aacaaaacca tatgttcttt gatgttgaaa aagaagatcc taattataca gatgttatcg
     1801 aattggattt atcaacagtt gaagcatcgc tttcaggacc aaaacgtcct caagatttaa
     1861 ttttcttaag tgatatgaaa tcatcatttg aaaattctgt aacagctcca gcgggcaacc
     1921 aaggacacgg tttagataaa agtgaatttg ataagaaagc agaaattaac ttcaaagatg
     1981 gatcaaaagc tacaatgaaa acaggtgata ttgcaatagc agcaattaca tcatgtacaa
     2041 atacatctaa cccttatgta atgttaggtg caggtttagt tgctaaaaaa gcagttgaaa
     2101 aaggcttgaa agttcctgaa tacgttaaaa cttctctagc accaggatca aaagttgtta
     2161 ccggatattt aagagatgct ggcttacaac cctatttaga tgaattaggc ttcaacttgg
     2221 ttggttatgg atgtacaact tgtattggta attcaggtcc tttattacca gaaattgaaa
     2281 aagcgattgc tgatgaggac ctattagtga catctgtatt atctggtaac cgtaactttg
     2341 aaggtcgtat ccatcctctt gttaaagcca attacctagc ttcaccacag ttagttgttg
     2401 cttatgcatt agctggaacg gttgatattg atttacaaaa tgaacctatt ggtaaaggta
     2461 atgacggtga agatgtatat ttgaaagata tttggccatc aattaaagaa gtttcagata
     2521 ccgttgatag tgttgtaaca cctgaattat ttattgaaga atataataac gtatacaata
     2581 acaacgaatt atggaatgag attgatgtaa ctgatcaacc tctatatgac tttgatccta
     2641 attcaacata cattcaaaat ccatcattct tccaaggatt atctaaagaa ccgggtacga
     2701 ttgttccgtt aaatggttta cgtgttatgg gtaaattcgg tgattctgtg acaactgacc
     2761 acatctctcc agcaggtgca attggtaaag atacgccagc tggtaaatat ttacaagatc
     2821 accaagtgcc tattcgtgaa tttaattcat atggttcaag acgtggtaat cacgaagtaa
     2881 tggttcgagg tacgtttgct aatatacgta ttaaaaacca attagcgcca ggtactgaag
     2941 gtggttttac aacttattgg ccaacaaatg aagtaatgcc tatctttgat gctgcaatga
     3001 aatataaaga agatggtaca ggtttagttg tattagctgg taacgattat ggtatgggtt
     3061 catctcgtga ctgggcagca aaaggtacaa acttattagg tgttaaaacg gttattgcac
     3121 aaagttatga acgtatccat cgttcaaatt tagttatgat gggtgtatta ccattagagt
     3181 ttaaaaaagg tgaatcagct gattctcttg gtctagatgg tacagaagaa atttctgtta
     3241 atattgatga aaatgttcaa ccacatgact acgtcaaagt tactgctaag aagcaagatg
     3301 gtgatttggt agaatttgac gctatggttc gttttgactc acttgttgaa atggattact
     3361 atcgccacgg tggtatttta caaatggttt taagaaataa attagcgcaa taaaaaatag
     3421 gtatcacagt aaatttttta tcggtatttg aggcactttg atagtgctct aaaaatagct
     3481 ctataaactt tatgatttaa taggtcggtt caaaaccttt gtgttcttgg ccggcctctt
     3541 cttgtatttt atgttagaat gagatataaa gttaaactga aagaaggctt aaaatgatat
     3601 atagtattac agaaatagaa gcgcgttatg ctgaaactga taagatgggt gtaatttatc
     3661 acgggaatta tgcaacttgg tttgaagttg cgcggttgga ttatatatcg aagttaggtt
     3721 ttagttatgc tgatatggaa aaacaaggaa tcatttcacc tgtgactgac ctcaatgtca
     3781 attataaaaa gtctattttt tatccagaaa aagttaaagt taaaacttgg gttgaaaaat
     3841 attcgagatt acgttcagtg tataaatatg agatttttaa tgaaaaaggt gaacttgcaa
     3901 ctacaggttc cacagaattg atttgcatta aagaagatac ttttaagcct atacggttgg
     3961 atcgttattt cccagattgg catgaagcct atagtaaagt gcaagcgctc aataatgaag
     4021 ggaaaatagt agagataatg gatggtattg attctttata agttactata aatgtttgga
     4081 gaggcaatat taataattaa tctataaaaa atagccaata cgttaacatt aatgtaatta
     4141 gcgtgaagat aagataatat atagcaaaaa cgaacttgtt catacaacat cagatgtgct
     4201 aaaccaaagt acaacttgcg aggttcagta cacatcagaa tgtgtaaaca agttcgtttt
     4261 attttactta ttttttggaa taagaaattt catcttcgtg atcaactaca tttacaataa
     4321 tgtggtcatc ttcaaagtac cacaaatctt tttctgctac gacaacattt aaatcgtcat
     4381 aatgttgttc atagccaata tcaacatctt cctttggttc aactgtaaaa gcaggactaa
     4441 atccttgctt gagttggaat tcgccaccat atcttacaaa aaacacgagc actttattat
     4501 tttcaggtaa ctcaagttca tttttaaacc aagttactgc tgcatcagta agttctattt
     4561 gcataacaat gacctcctat atttattaca gaatgggagt gggccccaat tcatattgat
     4621 tgtggaattt cttttcggaa ttctctgtgt tggagcccgc cccaacctgc acattattgt
     4681 aagctgaatt tccataagct tctatgttgg gacccccgat cagaattgaa aaaacctagt
     4741 tataagcgca tttcattctg tcaactacag ctaatataac attgtgtagc ctttgacatt
     4801 gatttatgtc tcggtctcaa atgttcttgt aaaaaaacta agtatagttg aattttaagt
     4861 taaaaaaatt gtttttgcaa aaataaaata atccatcaac tgcaaaaaaa tatgcaatga
     4921 tggactaatg tcaaaaaatt gttacatcca ttttatttta ggttcttcgc ctctaaaaat
     4981 ccttgcgata ttagagcgat gtctaattat caatatgatt gaaactaaga aactaacgac
     5041 taataaaata tagtcttgaa tgataagcga gccaatcaca cagcaaattg ctgcaacgat
     5101 acttgctaaa gaaacatatt taaaaatctt caatacaata aagaagataa ttgcaagtat
     5161 tagtaatagt atcgggttga ctcccaagac gacacctgca ctagttgcaa cagctttgcc
     5221 accttggaat tttaaataaa ctgggtaaac gtgtccaagt atagcgaata agcctacaat
     5281 taaaccattt gtaaaaaaag tactaatagg gccatctgcg tgaactggta accataaagg
     5341 gaagaaaaca gttatgaacc ctttgaaaat atctagaaat gttaccaaga atcctgcagg
     5401 acgacctaat actctaaagc tattagtagc gccagtatta ccactaccaa attgtctaat
     5461 atcttttttg aaaaataatt ttccaattac gaatccactt gggaaagcgc cgataagata
     5521 acttagtagt aacatgacga ttatcatcat aaatattaca catcctttaa tatcttagga
     5581 ctattttatc atattcgttt aaattacggc taaaattttt aaaaacgggg attaatatat
     5641 ggaattaagc tatgaaagtt aattgatact tgcattttac gctgatttat ataagaataa
     5701 ctattgtata gttttaaaaa cgaacgtacg tttgcaggag gcgaaatcat tggcaatgaa
     5761 taaacaaaat aattattcag atgattcaat acaggtttta gaggggttag aagcagttcg
     5821 taaaagacct ggtatgtata ttggatcaac tgataaacgg ggattacatc atctagtata
     5881 tgaaattgtc gataactccg tcgatgaagt attgaatggt tacggtaacg aaatagatgt
     5941 aacaattaat aaagatggta gtatttctac agaagataat ggacgtggta tgccaacagg
     6001 tatacataaa tcaggtaaac cgacagtcga agttatcttt actgttttac atgcaggagg
     6061 taaatttgga caaggcggct ataaaacttc aggtggtctt cacggcgttg gtgcttcagt
     6121 tgtaaatgca ttgagtgaat ggcttgaagt tgaaatccat cgagatggta atatatatca
     6181 tcaaagtttt aaaaacggtg gttcgccatc ttctggttta gtgaaaaaag gtaaaactaa
     6241 gaaaacaggt accaaagtaa catttaaacc tgatgacaca atttttaaag catctacatc
     6301 atttaatttt gatgttttaa gtgaacgact acaagagtct gcgttcttat tgaaaaattt
     6361 aaaaataacg cttaatgatt tacgcagtgg taaagagcgt caagagcatt accattatga
     6421 agaaggaatc aaagaatttg ttagttatgt caatgaagga aaagaagttt tgcatgacgt
     6481 ggctacattt tcaggtgaag caaatggtat agaggtagac gtagctttcc aatataatga
     6541 tcaatattca gaaagtattt taagttttgt aaataatgta cgtactaaag atggtggtac
     6601 acatgaagtt ggttttaaaa cagcaatgac acgtgtattt aatgattatg cacgtcgtat
     6661 taatgaactt aaaacaaaag ataaaaactt agacggtaat gatattcgtg aaggtttaac
     6721 agctgttgtg tctgtacgta ttccagaaga attactacaa tttgaaggac aaacgaaatc
     6781 taaattgggt acttctgaag caagaagtgc tgttgattca gtagttgcag acaaattacc
     6841 attctattta gaagaaaaag gacaattgtc taaatcactt gtaaaaaaag cgattaaagc
     6901 acaacaagca agggaagctg cacgtaaagc tcgtgaagat gctcgttcag gtaagaaaaa
     6961 caagcgtaaa gacactttgc tatctggtaa attaacacct gcacaaagta aaaatacaga
     7021 taaaaatgaa ttgtatttag tcgaaggtga ttctgcggga ggttcagcaa aacttggacg
     7081 agaccgcaaa ttccaagcga tattaccatt acgtggtaag gtaattaata cagagaaagc
     7141 acgtctggaa gatattttta aaaatgaaga aattaataca attatccaca caatcggggc
     7201 aggcgttggt actgacttta aaattgaaga tagtaactat aatcgtgtaa ttattatgac
     7261 tgatgctgat actgatggtg cgcatattca agtgctattg ttaacattct tcttcaaata
     7321 tatgaaaccg cttgttcaag caggtcgtgt atttattgct ttacctccac tttataaatt
     7381 ggaaaaaggt aaaggcaaaa caaagcgagt tgaatacgct tggacagacg aagagcttaa
     7441 taaattacaa aaagaacttg gtaagggctt cacgttacaa cgttacaaag gtttgggtga
     7501 gatgaaccct gaacaattat gggaaacgac gatgaaccca gacacacgaa ctttaattcg
     7561 tgtacaagtt gaagatgaag tgcgttcatc taaacgtgta acaacattaa tgggtgacaa
     7621 agtacaacct agacgtgaat ggattgaaaa gcatgttgag tttggtatgc aagaggacca
     7681 aagtatttta gataattctg aagtacaagt acttgaaaat gatcaatttg atgaggagga
     7741 aatctagtga gtgaaataat tcaagattta tcacttgaag atgttttagg tgatcgcttt
     7801 ggaagatata gtaaatatat tattcaagag cgtgcattgc cagatgttcg tgatggttta
     7861 aaaccagtac aacgtcgtat tttatacgca atgtattcaa gtggtaatac acacgataaa
     7921 aatttccgta aaagtgcgaa aacagtcggt gatgttattg gtcaatatca tccacacgga
     7981 gacttctcgg tgtacgaagc aatggtccgt ttaagtcaag actggaagtt acgacatgtc
     8041 ttaatagaaa tgcatggtaa taatggtagt atcgataatg atccaccagc ggcaatgcgt
     8101 tacactgaag ctaagttaag cttactagct gaagagttat tacgtgatat taataaagag
     8161 acagtttctt tcattccaaa ctatgatgat acgacactcg aaccaatggt attgccatca
     8221 agatttccta acttactagt gaatggttct acaggtatat ctgcaggtta cgcgacagat
     8281 ataccaccac ataatttagc tgaagtgatt caagcaacac ttaaatatat tgataatccg
     8341 gatattacag tcaatcaatt aatgaaatat attaaaggtc ctgattttcc aactggtggt
     8401 attattcaag gtattgatgg tattaaaaaa gcttatgaat caggtaaagg tagaattata
     8461 gttcgttcta aagttgaaga agaaacttta cgcaatggac gtaaacagtt aattattact
     8521 gaaattccat atgaagtgaa caaaagtagc ttagtaaaac gtatcgatga attacgtgct
     8581 gacaaaaaag ttgatggtat cgttgaagta cgtgatgaaa ctgatagaac tggtttacga
     8641 atagcaattg aattgaaaaa agatgtgaac agtgaatcaa tcaaaaatta tctttataaa
     8701 aactctgatt tacagatttc atataatttc aacatggtcg ctattagtga tggtcgtcca
     8761 aaattgatgg gtattcgtca aattatagat agttatttaa atcatcaaat tgaggttgtt
     8821 gcaaatagaa cgaagtttga attagataat gctgaaaaac gcatgcatat cgttgaaggt
     8881 ttgattaaag cgttgtcaat tttagataaa gtaatcgaat tgattcgtag ctctaaaaac
     8941 aagcgcgacg ctaaagaaaa ccttatcgaa gtatacgagt tcacagaaga acaggctgaa
     9001 gcaattgtaa tgttacagtt atatcgttta acaaacactg acatagttgc gcttgaaggt
     9061 gaacataaag aacttgaagc attaatcaaa caattacgtc atattcttga taaccatgat
     9121 gcattattga atgtcatcaa agaagaattg aatgaaatta aaaagaaatt caaatctgaa
     9181 cgactgtctt taattgaagc agaaattgaa gaaattaaaa ttgacaaaga agttatggtg
     9241 cctagtgaag aagttatttt aagtatgaca cgtcatggat atattaaacg tacttctatt
     9301 cgtagcttta atgctagcgg tgttgaagat attggtttaa aagatggtga cagtttactt
     9361 aaacatcaag aagtaaatac gcaagatacc gtactagtat ttacaaataa aggtcgttat
     9421 ctatttatac cggttcataa attagcagat attcgttgga aagaattggg gcaacatgta
     9481 tcacaaatag ttcctatcga agaagatgaa gtgattatta atgtctttaa tgaaaaggac
     9541 tttaatacag atgcatttta tgtttttgcg actcaaaatg gcatgattaa gaaaagtaca
     9601 gtgcctctat ttaaaacaac gcgttttaat aaacctttaa ttgctactaa agttaaagaa
     9661 aatgatgatt tgattagtgt tatgcgcttt gaaaaagatc aattaattac catcattaca
     9721 aataaaggta tgtctttaac ttataataca agtgaactat cagataccgg attaagggca
     9781 gctggtgtta aatcaataaa tcttaaagct gaagatttcg ttgttatgac agaaggtgtt
     9841 tctgaaaatg atactatatt gatggccaca caacgcggct cgttaaaacg tattagtttt
     9901 aaaatcttac aagttgctaa aagagcacaa cgtggaataa ctttattaaa agaattaaag
     9961 aaaaatccac atcgtatagt agctgcacat gtagtgacag gtgaacatag tcaatataca
    10021 ttatattcaa aatcaaacga agaacatggt ttaattaatg atattcataa atctgaacaa
    10081 tatacaaatg gctcattcat tgtagataca gatgattttg gtgaagtaat agacatgtat
    10141 attagctaaa aactatatgc aatcacgaaa ttaaatgata aaatacagta atgttaaatt
    10201 ttgactaaat tcaagggatt tatattaaat gctgaccaag tacttatcgt taaattagcg
    10261 atatactttg accaaactaa tataaatctc ttgtattgta taagaaatga tgatgtatct
    10321 ctatatttac tgaagtttca attcggtcta attttataga aggtgcatga ttcatgtaga
    10381 ggatgcgagg ggtttatttt gaaagatttc gatagtttaa ttcctggatg gtttaaagaa
    10441 tttgtccatg ttggtaccga tctaatatgg tctcaatatt taattggtct attattgaca
    10501 gccggattct tctttacaat tagttctaaa ttcgtccaat tacgaatgtt gcctgaaatg
    10561 tttagagctt tagtagaacg tccagaaact ttagaagatg gtaagaaggg tatttcgccg
    10621 ttccaagcat ttgcgattag tgctggttca agagttggta ctggtaatat tgctggtgtt
    10681 gcgactgcga ttgttttagg cggtccaggt gcagtatttt ggatgtgggt tattgcattt
    10741 ataggtgcag cgagtgcatt tatagaagcg actttggctc aggtttataa agtacatgat
    10801 aaagatggtg gattccgtgg tggtccagct tactatatta ctaaaggttt aaatcaaaaa
    10861 tggctaggta tcgtatttgc gattttaatt acaattacat ttgcatttgt atttaacaca
    10921 gtgcaatcta atacaattgc ggagtcgtta aatacgcaat ataatattag tccagtaatc
    10981 acaggtatta ttttagcaat cgtgacagct attattatat ttggtggtgt acgtagtatt
    11041 gctacgttat cttcgttaat tgtaccgatt atggctatca tttacattgg tatggtttta
    11101 gtaatattgc tatttaattt agatcaaatt gttcctatga taggtacgat tattaaaagt
    11161 gcatttggta tcgaacaagt aactggtggc gctgtaggtg ctgctgtgtt gcaaggtatt
    11221 aaacgtggtt tattctctaa tgaagctggt atgggttctg cgccaaatgc agctgctact
    11281 gctgcagtgc cacacccggt caagcaaggt ttaatccaat cattaggtgt gttctttgat
    11341 acaatgttgg tttgtacagc aactgcaatc atgattttat tatattcagg attaaaattt
    11401 ggtgataacg cacctcaagg tgttgcagtt acccaatcag cacttaatga acatttaggt
    11461 tctgctggag gtattttctt aacaatagca gttacattat tcgccttttc atctgtggta
    11521 ggtaattact attacggtca atctaatatt gaatttttat caacaaaccg tgtgatatta
    11581 tttattttta gatgtcttgt tgtagtactt gtctttgtcg gtgcagttgt aaaaacagaa
    11641 acagtatgga atacagcaga cttatttatg ggcttaatgg caattgtaaa cattatttcc
    11701 attataggac tgtccaatgt agcttttgca ttgatgaaag attatcaaaa gcagaaaaaa
    11761 gaaggcaaga atcctgtttt taaacctgaa aacttagaaa ttaacttatt tggaattagt
    11821 gcttggggcg ctaacaaata taagaactct gataaataaa tatttttaat gtaacgattc
    11881 attttataaa aatcaaataa accctgaggt tttcaaatta aagcgaaaat ctcagggttt
    11941 taaatttg
//
LOCUS       DAPIDM010000076        11743 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000076 DAPIDM010000000
VERSION     DAPIDM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.076"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            28..696
                     /gene="capA"
                     /locus_tag="RJE42_001734"
     CDS             28..696
                     /gene="capA"
                     /locus_tag="RJE42_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDV5877150.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            712..1398
                     /gene="cap8B"
                     /locus_tag="RJE42_001735"
     CDS             712..1398
                     /gene="cap8B"
                     /locus_tag="RJE42_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDV5877151.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            1401..2165
                     /gene="cap8C"
                     /locus_tag="RJE42_001736"
     CDS             1401..2165
                     /gene="cap8C"
                     /locus_tag="RJE42_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDV5877152.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLRQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGVSGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2185..4008
                     /gene="cap8D"
                     /locus_tag="RJE42_001737"
     CDS             2185..4008
                     /gene="cap8D"
                     /locus_tag="RJE42_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDV5877153.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGEIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3998..5026
                     /gene="cap8E"
                     /locus_tag="RJE42_001738"
     CDS             3998..5026
                     /gene="cap8E"
                     /locus_tag="RJE42_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDV5877154.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5039..6148
                     /gene="cap8F"
                     /locus_tag="RJE42_001739"
     CDS             5039..6148
                     /gene="cap8F"
                     /locus_tag="RJE42_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDV5877155.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNL
                     YGESKLQGEQLLRQYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNNRNVELTLNYVDDIVAEIKRAIEGNPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLNRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6152..7276
                     /gene="cap8G"
                     /locus_tag="RJE42_001740"
     CDS             6152..7276
                     /gene="cap8G"
                     /locus_tag="RJE42_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDV5877156.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7279..7905
                     /locus_tag="RJE42_001741"
     CDS             7279..7905
                     /locus_tag="RJE42_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDV5877157.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSCTTIENDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7910..9019
                     /locus_tag="RJE42_001742"
     CDS             7910..9019
                     /locus_tag="RJE42_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV5877158.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFAIGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKSIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEKVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            9033..10199
                     /locus_tag="RJE42_001743"
     CDS             9033..10199
                     /locus_tag="RJE42_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDV5877159.1"
                     /translation="MKFFVLCAIISMNIFLVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKVTVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFILGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSPLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILSLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10192..11397
                     /locus_tag="RJE42_001744"
     CDS             10192..11397
                     /locus_tag="RJE42_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDV5877160.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLFAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTMAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFIDKKDVD"
     gene            11398..>11743
                     /gene="cap8L"
                     /locus_tag="RJE42_001745"
     CDS             11398..>11743
                     /gene="cap8L"
                     /locus_tag="RJE42_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDV5877161.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILP"
BASE COUNT         4225 a         1581 c         2182 g         3755 t
ORIGIN      
        1 aatgaaaatt tttaagggag gtaaataatg gaaagtacat tagaattaac aaaaattaaa
       61 gaagtattac aaaaaaactt gaagatttta attattttac cgctattatt tttaattatt
      121 agcgctattg ttacattttt cgtcttatca cctaaatatc aagctaatac tcaaatttta
      181 gtgaatcaaa ctaagggtga caatcctcag tttatggcac aagaggttca aagtaatatt
      241 caacttgtaa atacgtataa agaaattgtt aaaagtccta gaattttaga tgaggtgtca
      301 aaggacttaa atgataagta ttcaccatct aaattgtcga gtatgttgac aattacaaat
      361 caagaaaata cgcaacttat caacatccaa gttaaaagtg gtcataaaca agattcggaa
      421 aaaattgcga atagcttcgc taaagttaca agtaaacaaa taccgaagat tatgagtgtg
      481 gataacgtat caattttatc taaagcagac ggtacagcag ttaaagtcgc accaaaaact
      541 gtagtgaatc taatcggtgc attcttttta ggattagttg tcgcgcttat atatatcttc
      601 ttcaaagtaa ttttcgataa gcgaattaaa gatgaagaag atgtagagaa agaattagga
      661 ttgcctgtat tgggttcaat tcaaaaattt aattaaggat ggttgctact tatgtcaaaa
      721 aaggaaaata cgacaacaac actatttgtg tatgaaaaac caaaatcaac aattagtgaa
      781 aagtttcgag gtatacgttc aaacatcatg ttttcaaaag caaatggtga agtaaagcgc
      841 ttattggtta cttctgaaaa gcctggtgca ggtaaaagta cagttgtatc gaatgtagcg
      901 attacttatg cacaagcagg ctataagaca ttagttattg atggcgatat gcgtaagcca
      961 acacaaaact atatttttaa tgagcaaaat aataatggac tatcaagctt aatcattggt
     1021 cgaacgacta tgtcagaagc aattacgtcg acagaaattg aaaatttaga tttgctaaca
     1081 gctggccctg tacctccaaa tccatctgag ttaattgggt ctgaaaggtt caaagaatta
     1141 gttgatctgt ttaataaacg ttacgacatt attattgtcg atacaccgcc agttaatact
     1201 gtgactgatg cacaactata tgcgcgtgct attaaagata gtctgttagt cattgatagt
     1261 gaaaaaaatg ataaaaatga agttaaaaaa gcaaaagcac ttatggaaaa agcaggcagt
     1321 aacattctag gcgtcatttt aaataaggca aaagtagata aatcttctag ttattatcac
     1381 tattatggag atgaataagt atgattgata ttcataacca tatattgcct aatatcgatg
     1441 acggtccgac aaatgaaaca gagatgatgg atcttttaag acaagcgaca acacaaggtg
     1501 ttaccgaaat cattgtaaca tcacatcact tacatcctcg atataccaca cctatagaaa
     1561 aagtgaaatc atgtttaaac catattgaaa gcttagagga agtacaagca ctaaatctaa
     1621 agttttatta tggtcaggaa ataagaatta ccgatcaaat ccttaatgat attgatcgaa
     1681 aagttattac cggtattaat gattcacgct atttactaat agaatttcca tcaaatgaag
     1741 ttccacacta tactgatcaa ttatttttcg aattacagag taaaggcttt gtaccgatta
     1801 ttgcacatcc agagcggaat aaagcaataa gtcaaaacct tgacatacta tacgatttaa
     1861 ttaacaaagg tgctttaagt caagtgacaa cggcgtcatt agcgggtgtt tccggtaaaa
     1921 aaattagaaa attagcaatt caaatgattg aaaacaatct gacacatttc atcggttcag
     1981 atgcgcataa cacagaaatc agaccgttct taatgaaaga cctatttaat gataagaaat
     2041 tacgtgatta ttatgaagat atgaacggat ttattagtaa tgcgaagtta gttgttgatg
     2101 ataaaaaaat tcctaaacga atgccacaac aagattataa acagaaaaga tggtttgggt
     2161 tataaacagc aaatgagggg ttttatggca catttatctg tgaaattgcg gcttttaata
     2221 ctagcattaa tcgattcact gatagtgaca ttttcagtat tcgtaagtta ttacatttta
     2281 gaaccgtatt tcaaaacata ttctgtcaaa ttattaatat tggcagctat atcactattc
     2341 atatcgcatc atatttcagc atttattttt aatatgtatc atcgagcgtg ggaatatgcc
     2401 agtgtgagtg aattgatttt aattgttaaa gctgtgacga catctatcgt tattacgatg
     2461 gtggtcgtga caattgttac aggcaataga ccgtttttta gattgtattt aattacttgg
     2521 atgatgcact tgattttaat aggtggctca aggttatttt ggcgtattta tcggaaatac
     2581 cttggaggta agtcatttaa taagaagcca actttagttg ttggtgctgg tcaagcaggt
     2641 tcaatgctga ttagacaaat gttgaaaagt gacgaaatga aacttgaacc ggtattagca
     2701 gtcgatgatg acgaacataa acgcaatatc acaattactg agggggtaaa agtacaaggt
     2761 gaaattgcgg atattccaga actagtgagg aaatataaga ttaaaaaaat catcattgca
     2821 attccaacta ttggtcaaga gcgtttgaaa gaaattaata atatttgcca tatggatggc
     2881 gttgagttat tgaaaatgcc aaatatagaa gatgtcatgt ctggtgagtt agaagtgaac
     2941 caacttaaaa aagttgaagt agaagattta ctaggcagag atcctgttga attagatatg
     3001 gatatgatat caaatgaatt gacgaataaa actattttag ttacgggtgc aggtggttca
     3061 ataggatcag aaatttgtag acaagtttgt aatttctatc cagaacgtat tattctactt
     3121 ggccatggtg aaaacagtat ttatttaatc aatcgtgaat tgcgaaatcg cttcggaaaa
     3181 aatgttgata tcgttcctat tatagcggat gtgcaaaata gagcgcgtat gtttgaaatt
     3241 atggaaatgt ataaaccata cgcagtttat catgcagcag cacacaagca cgtgccgtta
     3301 atggaagaca acccagaaga agcagtacgt aataatattt taggtacgaa aaatactgct
     3361 gaagctgcta aaaatgcaga ggtaaagaaa ttcgttatga tttctacgga taaagccgtt
     3421 aatccaccta atgtcatggg agcttcaaag cgaattgcag aaatgattat tcaaagttta
     3481 aatgatgaaa cacatcgaac aaattttgtt gcagtgagat ttggtaatgt acttggatca
     3541 agagggtctg tgattccact tttcaaaagt caaattgaag aaggtgggcc agttactgtg
     3601 acacatcctg aaatgacacg ttactttatg acaattcctg aagcttctag actagttttg
     3661 caggcagggg cattagcaga aggtggcgaa gtatttgtgc tagatatggg agaaccagtg
     3721 aaaattgtag atttggcacg taatttaatt aagctaagtg gtaaaaaaga agacgacata
     3781 cgcattactt atacagggat tagacccggc gaaaaaatgt ttgaagagct tatgaataaa
     3841 gatgaggttc atcctgaaca agtatttgaa aaaatttatc gtggcaaagt acaacatatg
     3901 aaatgtaatg aagttgaagc gattattcaa gacatcgtca atgactttag taaagaaaaa
     3961 attattaact atgccaatgg caaaaaggga gataattatg ttcgatgaca aaattttatt
     4021 aattactggg ggcacaggat cattcggtaa tgctgttatg aaacggtttt tagattctaa
     4081 tattaaagaa attcgtattt tttcacgcga tgagaaaaaa caagatgaca ttcgaaaaaa
     4141 atataataat tcaaaattaa agttctacat tggtgatgtg cgtgatagtc aaagtgtaga
     4201 aacagcaatg cgagatgttg attacgtatt ccatgcagca gctttgaaac aagtgccgtc
     4261 atgtgaattc tttccagttg aggcagtgaa gacaaatatt attggtacag aaaatgtctt
     4321 acaaagtgct attcatcaaa atgtaaaaaa agtcatatgt ttatctacag ataaggcagc
     4381 gtatcctatt aatgctatgg gtatttcgaa agcaatgatg gaaaaagtat tcgtagccaa
     4441 atcaagaaat attcgtagtg aacaaacgct tatttgcggt acaagatacg gtaatgtgat
     4501 ggcttcaaga ggatcagtaa ttcctttgtt tatcgataaa atcaaagctg gagaaccttt
     4561 aacgattaca gatcctgata tgacaagatt tttaatgagc ttagaagatg cggtagaact
     4621 agttgttcat gcatttaagc atgcagagac aggcgatatt atggttcaaa aagcaccaag
     4681 ctcaacggta ggggatcttg ctaccgcatt attagaattg tttgaagctg ataatgcaat
     4741 tgaaatcatt ggtacgcgac atggagagaa aaaagcagaa acattgttga cgagagaaga
     4801 atacgcacaa tgtgaagata tgggtgatta ttttagagtg ccggcagact ccagagattt
     4861 aaattatagt aattatgttg aaaccggtaa cgaaaagatt acgcaatctt atgaatataa
     4921 ctccgataat acacatattt taacggtgga agagataaaa gaaaaacttt taacactaga
     4981 atatgttaga aacgaattga atgattataa agcttcaatg agataggaga gattgacgtt
     5041 gaatattgta attacaggag caaagggttt tgtaggaaaa aacttgaaag cagatttaac
     5101 atcaacgaca gatcatcata ttttcgaagt acatcgacaa actaaagagg aagaattaga
     5161 gtcagcgttg ttgaaagcag actttgtcgt gcatttagcg ggtgttaatc gacctgaaca
     5221 cgacaaagaa ttcagcttag gaaacgtgag ttatttagat catgtgcttg atatattaac
     5281 tagaaatacg aaaaagccag cgatattatt atcgtcttca atacaagcaa cacaagataa
     5341 tctttatgga gagagtaagt tgcaaggtga acagctatta agacaatatg ccgaagagta
     5401 tggcaatacg gtttatattt atcgatggcc aaatttattc ggtaagtggt gtaagccgaa
     5461 ttataactca gtgatagcaa cattttgtta caaaattgca cgtaacgaag agattcaagt
     5521 taataatcgg aatgttgaac tgacgctaaa ctacgtggat gatatcgtcg ctgaaataaa
     5581 acgtgctatt gaaggaaatc caacgattga aaatggtgta cctacagtac caaacgtatt
     5641 taaagtgaca ttgggagaaa ttgtagattt attatacaag ttcaaacagt cacgtctcaa
     5701 tcgaacattg ccgaaattag ataacttgtt tgaaaaagat ttgtatagta cgtatttaag
     5761 ctatctacct agtacagact ttagttatcc cttacttatg aatgtggatg ataggggttc
     5821 ttttacagaa tttataaaaa caccggatcg tggtcaagtt tctgtaaata tttctaaacc
     5881 aggtattact aaaggtaatc actggcatca tactaaaaac gaaaaatttc tagtcgtatc
     5941 aggtaaaggg gtaattcgtt ttagacatgt taatgatgat gaaatcattg aatattacgt
     6001 ttctggcgac aaattagaag ttgtagacat accagtagga tacacacata atattgaaaa
     6061 tttaggcgac acagatatgg taactattat gtgggtgaat gaaatgtttg atccaaatca
     6121 gccagatacg tatttcttgg aggtatagcg catggaaaaa ctaaaattaa tgacaatagt
     6181 tggtacaagg cctgaaatca ttcgcttatc atcaacgatt aaagcatgtg atcaatattt
     6241 taatcagata ttagtacaca ctggtcaaaa ttatgattat acattgaatc aaattttctt
     6301 tgatgatttg gaattaagac aaccggacca ctacttagag gcagttggaa gtaacctcgg
     6361 agaaacgatg gggaatatta ttgctaagac atatgacgtt ttattacgcg aacaaccaga
     6421 tgcactttta attcttggtg atacaaatag ttgtttagca gcagtatctg ctaaacgatt
     6481 aaagattcct gtgttccata tggaagcggg taatagatgc tttgatcaga atgtacctga
     6541 agaaatcaat cgtaaaattg ttgaccatgt cagtgatgtg aatctacctt atacggaaca
     6601 tagcagacgg tatttattag atgaaggctt caataaagcg aatatctttg tgacaggatc
     6661 accgatgaca gaagtgatag aagcgcatcg agataaaatt aatcacagtg acgttttaaa
     6721 taaactagga ttagaaccgc aacaatacat tttagtatct gcgcatagag aagagaatat
     6781 cgataatgaa aagaatttta aatcattaat gaatgcgata aatgatattg ccaaaaagta
     6841 taaaatgcct gtgatttatt caacgcatcc aagaagttgg aagaaaattg aagaaagtaa
     6901 atttgaattt gatccattag ttaaacagtt aaagccattt ggtttctttg attataatgc
     6961 attgcaaaaa gatgcgtttg ttgtgctatc agatagtgga acattgtcag aagagtcgtc
     7021 tattttgaag ttccctggtg tccttattcg aacttccaca gaaagaccgg aagtactaga
     7081 taaaggtacg gttattgtag gtggtattac ctataacaat ctaatccaat ccgttgaact
     7141 agccagagag atgcaaaaca ataacgaacc gatgattgat gctattgatt ataaagacac
     7201 taacgtttcg acaaaagtag ttaaaattat tcaaagctat aaagatatta tcaatcgaaa
     7261 tacttggagg aaatgacgat gaggatagcg attgaaaaga taattggttt gctgaaaaac
     7321 cagtcctcta aagaatcgaa tgttaagatt catcgcttgg cgtatattac aaactcaaaa
     7381 tttgatggca ataactatat agatagatgg tgtaaaatca ggaattctca cattggtgaa
     7441 tacagttata ttggatttgg tagtgatttt aataatgtag aagtaggaag gtattgttcg
     7501 atatcttcgg atgtaaaaat tgggttagga aaacatccta cacacttttt tagctcatca
     7561 ccgatttttt attctaataa taatccattt aacataaagc aaaagtttat agactttaat
     7621 gaccaaccaa gctgtacaac aattgaaaat gatgtgtgga ttggtgcaaa tgtaattatt
     7681 atggatggtt taacaataaa tactggtgca gtcatagcag ccggctcagt tgttactaaa
     7741 aatgtaggag catatgaggt tgttggtggg gttcctgcaa aagtgattaa gaagcgattt
     7801 gacaataaaa caattgaaaa acttttagaa agcaagtggt gggagaaaac gcctgacaaa
     7861 ctaaaaggat tttcggttga atatttaaat aaaaaggata cttaatgata tgagaatttt
     7921 aaatattgta tcgagtaata ttgttcaaga cccaagggta cttaaacaaa tagaaacaat
     7981 taaaggcgtt acgaatgatt ataaaattgt tggaatgaat aattcacaag ctactaataa
     8041 gcgattggaa aatttagatt gtaattatcg tttgttaggt agcaaggtag atccaaaaaa
     8101 tattctttct aaattaatta agcgtataag atttgcaata ggtgttatcc gagaaattaa
     8161 agcttataaa cctgacgtga ttcatgcaaa tgatttcgac gtattattaa tggtttattt
     8221 aagcaattat aaaaaagcta atattgttta tgatgcgcat gaaatatatg cgaaaaatgc
     8281 ctttattaat aaagttccac ttatttcaaa gtttgtagaa agtatagaaa aacacattgt
     8341 aaaacatcgt gttaatgcct tcgtaacagt aagtcatgca gcaaaagaat attatcaatc
     8401 taaaggatat aagaaggaag cgaatgttat tacgaatgca cctattttaa atgatagcag
     8461 agaatttaaa gaaatcgaaa actttaaatc aatcgtatat caaggtcaaa ttgtaatgga
     8521 tagaggatat gaagagttta ttattgcttc atcagctttt aaacaaaatg ccccttcatt
     8581 tataattcga gggtttggtc cgcatgaaaa agtgataaaa gaactgatta gttataaccc
     8641 ggaaaatatt aggttggata aaccagttga agtaaaagaa ttggttgata agttagcaga
     8701 aagtaatgtt ggtgttgtct tgacgaaacc tgtatctatt aattttgaat atacagtatc
     8761 taataaaatt tttgaatgta tacatgctgg tttaccagta attttatctc ctgtcaaaga
     8821 gcatatttat ctcaatgaaa aatataaatt tggcattgtt ctaaaggaag ttacgccgtt
     8881 agaaattgaa aaggcggtta gaaaactaag agataatcac gatttgttta atcatttacg
     8941 tcaaaatgca attaaggcgt ctaaaatttt gaattggcaa atagaaagtg aacgattagt
     9001 agaattatat aaattttaaa gagaggtaga ctatgaaatt ttttgtactt tgtgcaatta
     9061 tcagcatgaa catattttta gtaatctcta catttactaa agaagtatta ggattcccta
     9121 tagagccggt gtattactca accatggttg gtatagcatt aattactacg gtgtttgcta
     9181 tttataagat aattgtcacg caagaaattc cgagagggtt aatattatta attgctatat
     9241 gtttgcttta tctagctttt tattattttt caccagataa ggaagagaaa ctagctaaaa
     9301 ataatattct attcttttta acatgggcag ttccagcggc aattagtggt atttatatta
     9361 aatatataaa caaggttaca gtagaaagat tttttaaatt agtatttttc atattttctg
     9421 tttcatttat ttttgtaatt ttaataccaa aacttacagg tgagatacct agctatatca
     9481 attttggact tatgaactat caaaatgctt cgtacctttc agcatttact gccggattag
     9541 gcatttattt cattatgaaa ggctcagtta aacataagtg gatatatgtt ctatttacaa
     9601 taattgatat ccctattgtg tttatactag gagggcgtgg aggtgctatt ttattaattc
     9661 tttacggctt gtttgcattt atacttatta cgtttaaaag aggaatacct atagcagtaa
     9721 aaagcattat gtatattttt gcattaagca tatctagtgt attgatttac tttcttttta
     9781 caaaaggttc gaatactaga acattttcat atctacaagg tggaacactt aatttagaag
     9841 gtacttctgg aagaggaccg atttatgaaa aaggtattta ctttattcaa caaagtccgt
     9901 tattaggcta tggaccattt aactactata aactaatcgg aaatatacca cataacatca
     9961 ttattgagtt gattctatca tttggcttat tagggttttt tatcataatg atttgcattt
    10021 tgtcactagt ttataaaatg attaggaact atgatccaaa cactatagat ttactcgtta
    10081 tgtttatagc aatctatcca atcacattat taatgtttag ttcaaattat ttagttgtaa
    10141 gtgaattttg gtttgtgttg ttctatttta ttacaaaagg acggcgtcat catggctaag
    10201 aaagttttta ttatggatag cgtaaagaca ataattggta cgttgcttat agctttagga
    10261 ttacaatttt tagcttatcc aattattaat caacgagtag gtaatgaagc gttcggttct
    10321 attttaacga tttatacaat aataacaatc acgagtgttg tattaggcaa tacgcttaac
    10381 aatatacgat tgattaatat gaatctatac aaatccaatc attactactg gaaatttgca
    10441 tcgatacttt taatctcaat tctgattgag agtatagctt taattattgt atttctttac
    10501 ttttttaatt tgaacatcat cgatattatc tttttaattc tacttaatat tttaatgtgt
    10561 ttaaggattt atctgaatgt attttttagg atgactttaa aatataatca gattttgtat
    10621 attgctctta ttcaattttt aggtttgctg ataggactat ttctatatta tttaacccaa
    10681 aactggattg tttgttttat taccagtgaa ttgtttgcaa cgatatatac attggttaaa
    10741 ttacggggat taactatagg cgagtatcaa agtgaagata ataatgtggt aaaagattat
    10801 gtgatgctac tgagtacaaa tagccttaat aatttgaatc tctacttaga tagattaatc
    10861 ttattaccaa ttataggtgg gacagctgta actatatcat ttctttcaac atttattggg
    10921 aaaatgttag ctacatttct gtatccgatt aataatgtag tactttcata tatttctgta
    10981 aatgaaagtg acaatataaa gaaacaatat ttgaaaacta atctatttgc tatagctgcc
    11041 ctatgtttag tcatgattat ttgttatcca attacaataa ttattgtctc tttactgtat
    11101 aacattgatt caagtttata ttcgaagttt attattttag gtaatatagg tgttttattc
    11161 aatgcagtga gtattatgat ccaaacttta aatacaaaac acgcatcaat aacattacaa
    11221 gcgaattata tgacgcttca cacgattaca tttatattca taactatttt aatgacaatg
    11281 gcgtttggtt taaatggatt cttttggaca acgctgttca gcaacattat taagtatgtg
    11341 attttaaata ttataggttt aaagtctaaa ttcattgata aaaaggacgt cgattagatg
    11401 agtgaaaaaa agattttgat tttatgtcag tatttttatc cggaatatgt atcttctgcg
    11461 acgttaccaa cacaattggc ggaagattta attgcgaatc acattaatgt cgatgtcatg
    11521 tgtggatggc catatgaata tagtaatcat aaacaggttt ctaaaaccga gatgcatcgt
    11581 ggtattcgca ttcgacgtct aaagtattcg aggtttaata acaaaagtaa ggttggaagg
    11641 atcatcaatt tctttagttt attttcaaaa ttcgtgatta atatacctaa aatgttgaaa
    11701 tatgatcaga ttcttgttta ctctaatcca ccaatcttgc cat
//
LOCUS       DAPIDM010000077        11717 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000077 DAPIDM010000000
VERSION     DAPIDM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.077"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(296..1123)
                     /locus_tag="RJE42_001746"
     CDS             complement(296..1123)
                     /locus_tag="RJE42_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877162.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(1126..1245)
                     /locus_tag="RJE42_001747"
     CDS             complement(1126..1245)
                     /locus_tag="RJE42_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877163.1"
                     /translation="MMLISFVYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1357..2358
                     /locus_tag="RJE42_001748"
     CDS             1357..2358
                     /locus_tag="RJE42_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDV5877164.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2486..2944)
                     /locus_tag="RJE42_001749"
     CDS             complement(2486..2944)
                     /locus_tag="RJE42_001749"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV5877165.1"
                     /translation="MNFEGKLIGKELKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAYVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSI"
     gene            complement(2957..4138)
                     /locus_tag="RJE42_001750"
     CDS             complement(2957..4138)
                     /locus_tag="RJE42_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDV5877166.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLIQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(4149..4781)
                     /locus_tag="RJE42_001751"
     CDS             complement(4149..4781)
                     /locus_tag="RJE42_001751"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV5877167.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4788..5831)
                     /gene="ribD"
                     /locus_tag="RJE42_001752"
     CDS             complement(4788..5831)
                     /gene="ribD"
                     /locus_tag="RJE42_001752"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV5877168.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRDHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5958..6093)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6312..7814)
                     /locus_tag="RJE42_001753"
     CDS             complement(6312..7814)
                     /locus_tag="RJE42_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDV5877169.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSANLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFEKLVHRRTGNHIADLKYDLARLTEMGIFQSIIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YKVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQEQRSDNQ"
     gene            8465..8779
                     /locus_tag="RJE42_001754"
     CDS             8465..8779
                     /locus_tag="RJE42_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV5877170.1"
                     /translation="MTYKELATYLKVLSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            8779..10071
                     /gene="arsB"
                     /locus_tag="RJE42_001755"
     CDS             8779..10071
                     /gene="arsB"
                     /locus_tag="RJE42_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDV5877171.1"
                     /translation="MMTTLATFIFLVTLLFVLWQPKGLDIGFTALAGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKIGFFEWSAIHMLHASKGSGLKMFVYIIL
                     LGAVVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFNISFSQYLSRMIIPNLFSLLASLLVLWLYFRKAIPKSFDANHIKKPID
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLILARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVIFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFFTLIGLYLTLILF"
     gene            complement(10158..11012)
                     /locus_tag="RJE42_001756"
     CDS             complement(10158..11012)
                     /locus_tag="RJE42_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDV5877172.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLKK"
     gene            complement(11288..11512)
                     /locus_tag="RJE42_001757"
     CDS             complement(11288..11512)
                     /locus_tag="RJE42_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877173.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT         3746 a         2058 c         1594 g         4319 t
ORIGIN      
        1 atattaccat taaattgcaa caattctaca tatatttctt caaaaaagat acgcattgaa
       61 tttttattac atataactct catgaaaatt gattaaacta aattaattag taagccttaa
      121 atttaacaaa ttaaaccaaa ccgaatgagg ctaaattggt tatttttcaa gatatgattt
      181 gtataaatca aaacatttct tacaaatcaa atgtgtataa aatgtgatat acattctcaa
      241 ccttaaaaaa gtttaaaaaa aagatgagca tctactgtct catcttttta gaatattaaa
      301 tttctacaat ttcgtcatct tcaacaataa agcccattgt attgacgcta ttatttaaga
      361 aagtcagaat ataacgcatt acttcatcgc gttctggctc attgtgaacc tcgtggtaaa
      421 aaccttgcca agctttaaaa tataattcag gtgtttgata tttttcttta aactcatcaa
      481 ttgccctagt atcaacaatt aaatccttcg ttccatacat taatagtgtt ggcattggtt
      541 gaatgtcatg aatatgagcc atcgtatctt tcatcgtctc attaattgtg ttataccaat
      601 gatacgttgc tttttttaac attaaaccat cgttaactgt ttcttcaaca atttctaaat
      661 tacgtgttaa atcttttggt tctacaccaa cattaaaacg tgtgtctttt gaaattttac
      721 ctatatttga aacaagttta tctttacgat tttttccatt cttttgaagt tctagcatag
      781 gagaaattaa catcatcccc tcgattggca attctacttt ttcaagtaaa tttaataaaa
      841 tcaaaccgcc aagtcctacc cctaatacat aagtaggaat tttatattca ttagctatct
      901 ttaaccagtc tagcaaactt tcgtgatacg tttgaaagtt ttcaatttgt cctttattag
      961 ctcttgaagt ttgaccttga ccaggcaaat ctcccataat cacatgatag ccatttcttc
     1021 ttaacatcgt aataacatat gcatatcttc ccgtatgttc taatatatta tgagcaataa
     1081 caacgacgcc tttcgcatca ttttcagctt cccacttcca cattattata ctgccccttt
     1141 ttcattaatc ttcaataaca taattatagc aaattcacta tgtagatttc tatttatagt
     1201 attattgttg tccatattat tatatacaaa tgaaatcaac atcaataata gtgtaattat
     1261 acataattat ttttgattgt ttttgatgaa aacgctttct cgaatatttt tttcatgcta
     1321 aacttattgt aaacacaagg gtttggagga gtagcaatgg cactattaaa gaattttttt
     1381 atcggattat ctaataatag ttttttaaac aacgcagcaa aaaaagtggg cccacgtttg
     1441 ggtgccaata aagtcgttgc cggaaataca attccagagt taattaatac aatcgaatac
     1501 ttaaatgaca agaatatcgc tgttacggta gacaatttag gggaatttgt cggtacagtt
     1561 gaagaaagta atcatgctaa agaacaaatt ttaacaatta tggacgcgct tcatcaacat
     1621 ggcgtaaagg cacatatgtc tgttaaattg agtcagttag gtgcagaatt cgacttagaa
     1681 ttagcttacc aaaatttaag agagatttta cttaaagcaa atacttacaa caatatgcat
     1741 ataaatattg atactgaaaa atatgctagc ctgcaacaaa ttgttcaagt tttagatcga
     1801 ttaaaaggcg aatttagaaa tgttggtact gtaattcaag catatttata cgatagccat
     1861 gaattagttg ataagtacca agatttacga ttacgtttgg ttaaaggtgc atataaagaa
     1921 aacgaatcaa ttgcatttca atctaaggaa gacgtagatg caaattacat caaaataatt
     1981 gaacaacgtt tgttaaacgc acgcaatttc acttcaattg caacacatga ccatcgcatt
     2041 attaatcatg taaaacaatt tatgaaagaa aatcacattg aaaaagatcg tatggaattc
     2101 caaatgctct atggttttag atcagagtta gcagaagaaa tcgcaaatga aggctataat
     2161 ttcactattt atgtacctta tggcgatgat tggtttgctt attttatgag aagattagca
     2221 gaacgcccac aaaacctatc tcttgctgta aaagaatttg tgaaacctgc tggcttaaaa
     2281 cgtgttggca taattgcagc tttaggagct acagttatgt taggtttaag tacaattaaa
     2341 aaattatgcc gtaaatagag catggcataa acaataattt aggagtctgg aacaataatc
     2401 aatgttctag gctcctaaat gttatattgg cagttctgaa tcatttataa atctttgatt
     2461 atgaactcac aacagcactc tatgcttata tagattttaa taaattagcc atttcaattg
     2521 cacttactgc tgcttcggca cctttattgc cagctttcgt acctgctctt tcaacagctt
     2581 gttcaatact ttcagtcgtt aaaataccaa atatgactgg tacattagtt tgatcattta
     2641 ctttagaaac accttttgcg acttcattac aaacataatc ataatgagaa gtagcaccgc
     2701 gaattacgca tcctaatgta attactgcat cataatttcc tgatgaggct aattttttag
     2761 ctactaaagg aatttcaaac gcaccaggta catatgctac atcaatatta tcttcattaa
     2821 catcatgtcg aatcaaagta tcttttgcac cttcaagtaa tcttccagtg ataaaatcat
     2881 taaatcgact aactacgatt gcaactttca attcttttcc aattaattta ccttcaaaat
     2941 tcatgttaaa gtcctcctat attaaatgac ccatttttat ttttttcgtt tccatataat
     3001 catgattatg taccgtttct ggtacgataa cttcaattct ttctgcaata tcaatgccat
     3061 attgtattaa tccttcgaat ttacttggat tattacttaa taaattgata tgttcgatgt
     3121 taaaatattt taatatctgt gcagcaatat gataatctcg caaatcttca tcaaaaccta
     3181 atgctaaatt tgcagttact gtatcatatc cttgctcaat taattcatat gcgcgtaatt
     3241 tgtttaacaa tcctatgcca cgaccttctt gaggtagata aataatcatg ccaccatgtt
     3301 cattgatata cttcatagac gattcaagtt gagcaccaca atcacaacgt tgactatgga
     3361 aaatatcgcc tgtaaggcac gcagaatgta aacgtacatt ttcatgttgt cgaattgcac
     3421 cttttgtcag tacaactatc tcttcatctg tgtaagtagc tttaaaacca tacatatcaa
     3481 atgttccgaa atcagtaggc attttcactt ttgccttaaa ttcaatttct ggttctaatt
     3541 ttttacgata ttcaattaaa tcatcaatcg taatcatctt taattgatgt ttttctttaa
     3601 acttttgtaa atcttgtcct ttcgccatcg tgccgtcatc attcataatc tcacaaatga
     3661 caccagctgg cttggcacca gtaagtttag ctaaatcaac agccgcttca gtatgaccat
     3721 tacgagctaa taccccttta tcttgtgcta ctaatggaaa taaatgacca ggacgattaa
     3781 aatctttagc ttcactactt ggatcaatga gctttttggc agtcaatgta cgttcataag
     3841 cgctaattcc tgttgttgta tctacatgat caatactcac tgtaaattgc gtaccaaaga
     3901 tgtcggagtt atcatcaacc atttgtacca aatccaaacg ttgtgcaata tctttagaca
     3961 ctggtgcgca tattaatccc cttgcttctt tcgccataaa attaatggta ttatcgttca
     4021 tccattcagt gaccgctact aaatcacctt cattttcacg attctcatca tctactacaa
     4081 taattgtttc tccatttttt aaagccatta aagcactgtc aatattatcg aattgcatgc
     4141 tacccctcct aaaaaccaaa tgctcttaat ttatctacag ataattggtc tttatcttta
     4201 tttaaaatat tttcaacata tttaaacaaa acgtctgttt ctaaatgtac tttatctcct
     4261 aattttttgg atgataaaat cgtagaacgc ctcgtttctg gaataagatg aatgtcaaaa
     4321 ctgttatcat gtttgtcaaa taccgttaga cttacaccat ccacagtaat agatccttgc
     4381 ttaactaact gattattaat atgttggcta cattgaatcg taataatttt ggcattggct
     4441 gtttcattta tttttgaaac tgttcctagt tcatctacat gaccgaggac aaaatgtcca
     4501 ccaaacctac cattaccact catggcccgc tctaaattta cttctgattg tcgcttaaca
     4561 tctgctaaat aggttttatt ttcagtgcct ttaattactt gtacagtaaa agatgtctga
     4621 ttaaaatcaa tcactgttaa acatgcacca ttaacactga tggaatcacc aatatgcata
     4681 tctgccgtaa ttttatgtgc ttcgatttca atcgtcctga ctgattgacg aatttgaaca
     4741 cttttaacga cacctatttc ttcaacgatg ccagtaaaca tgcatcatca cttctttcgt
     4801 aaagttaatt tgacattttg atttaataac tcggaatgaa caatttcaaa ttggttcgca
     4861 tctggtatct caatcacatc atttgtttga taaaattgat aatttccaga tccgccaatt
     4921 aatttcgggg cataatagag aataaattcg tctatataat tagattggag aaattctgaa
     4981 gtagtggttg gacctgcctc gactagcaaa gttccaactc ctcttttata taaattgtga
     5041 agaattgttg ttaaatcaca agacttcaag taaataattt caatatgtgt ttgattggtt
     5101 gttaaatttg gattttcagt atatatccaa attggtgttg attcatcttg ataaatttgc
     5161 tgattaaaat gaatattccc agacttagac aatattactt ttatagggtt ttttccatct
     5221 tgaatacgtg tagtatattg tggatcatct aactcaactg tacgtcttcc agttaacact
     5281 gcgtcgtgtc gatgtcttaa cttatagaca tcttgtttaa cctctttgtt agtaatccat
     5341 tgactttgtc cattatcatt cgcttgttta ccatctaaac ttgcagatac tttcactgta
     5401 atttgtggca gttgctttgc ttttgcttta aaaaagtctt ggtataattg tgatgcacgt
     5461 tcatcatcaa cgcattcaac ctcaataccg tgatcccgta acgtctcatc accatgtgta
     5521 tctaacgaat tgtcttttgt tgcgtaaact actttagcta tcttacaatc aattattttg
     5581 ttaacacagg gtggcgttga accaaaatga ctacatggct ctaacgtaat ataaatcgtc
     5641 gcaccttcag cattttgttg tgccatatca agtgcttgaa cctccgcatg cttgtcacct
     5701 tttctcaagt gtgcaccaat accaacaatc ctaccttctt taactacaac agcgccaacg
     5761 ggtggattaa cacctgtttg accttgtacc atatttgcaa gttgaatcgc ataatccata
     5821 aattgactca aatgatcacc tctataaaca aaaatcctca catcatgaat taagatgcaa
     5881 ggagaaaaat ttatcgttaa ataagcctat ttgtacacat ttttacaaat acgctacatt
     5941 atctttgtcg ataattaaca ttctttctcc catccagact ttaactgtcg gctctagaat
     6001 ctcactagat cagccactaa tatgaaacat attagcaggt cgcaggcttt atttactgcc
     6061 ggttgggaat ttcaccctgc cccgaaagaa ttatatatga aattgttata gattatttga
     6121 gtacgtagta tgtcaactac atttaaaatg atactatatg ttttctgaaa aaacaattaa
     6181 tgacggtttt aatttaatat aatctgagta ctataggcat ctcattgcta tgattcttac
     6241 taacagacat taaaatcaaa tcctcaatac gtctctatag agcgttctct ttattatctt
     6301 ctagttacaa attattgatt gtcacttcgc tgttcttgct cattcgattc taaagcatca
     6361 tataattgag atactgtatg cgcaacttgt tctactatca ttttcacacc atttcgtagt
     6421 ttattaacac cgtttgtcat ttgacctatc gcaatcatat ttgttaatgt tccaaacctt
     6481 ggactaataa cttgattggt ttccggtatg atttgtatac ctcccattgg atgtgcttgt
     6541 acaatttgtc tattttcaag atttttaatt aattgatcat cttgatccaa ttcatttaaa
     6601 tgactttttg cacctgtcgc gttaatgaca actttatata tgtctactga ttcttggttt
     6661 ttgtatgaaa aataatacaa cttgccatca tgtttcacat cttctaaatc ttttttcaaa
     6721 attaaagact tattttctat taattcaata attagttcag cagttcttgg aggcattgga
     6781 tttgaattta attgaatcat ctttgagtat ttttgattaa attgatgttg gtcttcaatg
     6841 cttaagctat tccatatcca atttaaattc tctttcaaat gttcaatgat actttggaaa
     6901 atgcccattt ctgttagacg cgctaaatca tacttcaaat ctgcaatatg atttcctgta
     6961 cgtctatgta ctaatttttc aaaatcaatg tcatattcag cacattcttt taaaaataaa
     7021 gaaactaacg tatcaagcgg tgcattgccg aaatgatgtt tttttatgtc atttaatttg
     7081 tctttagtta agtacttgaa tgtcacgtct atcattgtac ctcttacact tggtaaattt
     7141 gcagaacgac tcgtcatagt aattggtaat ttcggatgat gagcagcaac ataacgaaca
     7201 acatctaaac tggcaaggcc tgtaccaata atcgcaatat cgtccagttc atttacttcg
     7261 tctaacgtat tatatgttgg ataaggtgta gcgatatatc cttttttgcc cttcaagtta
     7321 tatggatcat ggtaagcaaa tgtaccacat gttaaaaata cataatcgta cgcttgccat
     7381 gattgttctg aatttgtagt acatatgtaa taagttaaat tcgtttcatc gatattagaa
     7441 tttgtataaa tctcttgaac tttattataa ttagttgata tatttggata ttttttcgtg
     7501 aacatagata aataagattt catataatgt ccgaatacaa atctcggtaa atatgcaggt
     7561 tcatcaaaat taaaatcagt ttgctgttta taccacttcc aaaattcagt ctcatcatct
     7621 aaatttaaac tcatcttttt cgaaggcata ttaataagca gctcagaact atcattttga
     7681 aatggtacgc cctgtcccat atttacttta tcatcgtata aatctatatc taattgatta
     7741 aatttcgggt gcttaactaa ctctctcaat acacttacac cagcagttcc catgccgatt
     7801 attgctacac gcatatatca tccatccttt agtcatttta aatattttct tttatctatt
     7861 acccatattt atagcaattt ggtattttta ataaaattta ttaaggtaat ctattatttc
     7921 acttttacaa ccactaaacc atttaaaact tttcatttaa tatcaaatct agtgtttgtg
     7981 ttaaaatatt tttaacaatt tgccatattt tatgaattca ccattgtcat acttcattac
     8041 ttcattactt catcataatt atgattcgtt ttaaagatgt tcattcaata tcgacaactg
     8101 cttatgtgtt aaaatatttt taacgaggtg aattaaattg aaatttatag caaagatatt
     8161 aatctcatta gtaattctat ttatgatttt tagtcgtaaa aataaagctt cttaatcaat
     8221 acccttgatt acaaaataaa ttcaactaag gtgattggcc atacactcaa gatatggcta
     8281 atcacctttt tcaatgcaga ataaagagta tattttgcaa aatcaatatt tataatttat
     8341 ctttaatacc cttttaatac taaaaccaaa tttaaattac atagtcatat cgtgaaatat
     8401 accaaagaaa catagacatt tatctatata gatgataatc tatttaacgt ctaggaggta
     8461 tgttatgacg tataaagaac tagcaacata tttaaaagtt ttatcagatt caagcagatt
     8521 agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt tagaacattt
     8581 tcaattctct caacccacac ttagctatca tatgaaagca ttagtaaaaa ccaacttagt
     8641 tacgacacga aaaatcggaa ataaacattt ataccagctt aatcataata tttttgagtc
     8701 cgtaattaat aacttgtcaa aagttcatac ctctaaccaa cgatgtattt gtcataacct
     8761 taagactggt gaatgctaat gatgacaact ttagcgacat tcatttttct agtaacttta
     8821 ttatttgtat tatggcaacc taaaggccta gatattggat ttactgcatt agcaggtgcc
     8881 tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt aacaggtata
     8941 gtctggaatg ccactttaac ttttgtctca gtcattctta tttcattgat attagataaa
     9001 attggatttt ttgaatggtc agcgatccat atgttacatg cttcaaaagg tagtggccta
     9061 aaaatgttcg tttatatcat attattgggt gccgtggttg ctgcattttt cgcaaatgat
     9121 ggcgcagctt taatcttaac gcccattgta ttagcaatgg taaaaaatat aggttttagt
     9181 aagcgggcca tatttccatt tattattgca agtggtttta ttgctgatac aacgtcttta
     9241 cctttaatcg tgagcaattt ggtcaatatc atttctgcag attactttaa cattagtttc
     9301 tctcaatatc ttagtagaat gattatacct aatttattct cacttttagc tagccttcta
     9361 gttttgtggt tatattttag aaaagcgatt cctaaatcat ttgatgcaaa tcacataaaa
     9421 aaacctatag atgccattaa tgatttaaag ctatttaaaa tttcatggat tgttctagtt
     9481 atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc aattttcacc
     9541 ggaatcattg cttttatatt tctaatattg gctcgtaaat caaatgctgt aaatattaag
     9601 caagtcatta agggcgcacc ttggaatata gtattatttt caattggtat gtatatcgtc
     9661 atattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt agaatatatt
     9721 tccaattacg gtctatttag caccattttg ggaatgggct tcatttcagc atttttatca
     9781 tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg tcaatcaaat
     9841 gtccatggca tgttaaaaga aggcctaatt tatgcaaatg ttatcggttc tgatttaggt
     9901 ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt cttaacacaa
     9961 aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat tacaattcca
    10021 gtactatttt tcacactcat aggattgtat ctaacactta tcctatttta agaaataaaa
    10081 agacgctttc tatttattgc gatagaaagc gtctttttat acttgagcaa gtcatatcat
    10141 aacttaactc tatcaaatta tttcttcaaa tgtttactgt catctttata tacaaagtat
    10201 ttgaagtatt ttccttcagt cttcatgttc ttataaaagt cagcgataat tgttgcatta
    10261 ctttctgccc acttaatatc agtagcatat tgatgttctc ctggattttt tggattccat
    10321 ctcatactat acaatgtatt ttgatctgtg cttgataaga agtgcttatg aatgaaatca
    10381 gcaccgcctg aaatagcttt ttcaggtgta tcccaaccat gctttttagc atattctgca
    10441 cctgttttaa ttgggtcttt atcaagggct cctactccat agaaattgta gtattttttg
    10501 ccatcaattt cgactccatt agctaattca cttttaactg cgccagtttc taataatgca
    10561 tgtgaaatta aataaacttc gttaacgtgc ttatctttag cagcttttaa gaaatcatcc
    10621 gtatgtttca ataacgttgg tctatcgact aacatacgtt taattctatt tttatcaatc
    10681 ccttgatact ttgataaatc taaaaattga tatttttgct tttcattatc gataaaagta
    10741 ccgctatcca ttgcactttt aatttcagtt gcagatgcat ctctccatgc atcattcttt
    10801 ttatttgata cctgttgact cgtataatta tttatttgtt tctttgctgc atcgtttaat
    10861 gtaacattta acttttcaat cttaatgtcg gatttaacat gtttgaaaaa tatctgatcg
    10921 gatatcattg agaaaaataa aaatgagaca acagcaaata tgacaacaag tcctattatt
    10981 ccaaaaatag aacctttctt gtgtttattc atatccacac ctcttaggtc attgttgtta
    11041 tacattcagt ttgactacga taataaagtt taacattatt aaaaccgttt aacaatttta
    11101 tctatgcatt gttacatatt cataaataaa cggtaatgta aatgaaacgt tttaaacgat
    11161 ttatcaacat tactttacgt tgattcacga atcataaatg ttttatcttt ttgtataacc
    11221 tttgaaaacc tttatatcaa taatcttatt attcacgtat taaaaaacaa aaaattctct
    11281 tgttcgttta cttagtttct tttttataat ccatcataat aaaacttgct gcatttgatc
    11341 tgtttttata atagtactga tttaaatcaa tcgtaccttc aatttgacca tcacgataca
    11401 tcatttcatt agtgaagtta tttatcatca cgaaatcagc gcgatctctt ataacatcta
    11461 aatcgtcgtt tcgtgcaaaa aaatgttcta aatttaaatg tgcgtaatcc attgttacct
    11521 cccgattaca aattttgttc tatctactat ttagcaaagt aataatacat catatcacat
    11581 taaattaatt acttatgtct tatatttaac acttaattta gcgattacgc aaatgaatga
    11641 tttttctaaa tattttattc taattttatc cggaaatatt tgttatacgt ataacttgga
    11701 gaatgactac ttgaaat
//
LOCUS       DAPIDM010000078        11178 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000078 DAPIDM010000000
VERSION     DAPIDM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.078"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..422
                     /gene="deoC"
                     /locus_tag="RJE42_001758"
     CDS             <1..422
                     /gene="deoC"
                     /locus_tag="RJE42_001758"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV5877174.1"
                     /translation="QNGADEIDMVINIGALKDGRFDDVQQDIEAVVKAAKGHTVKVII
                     ETVLLNHDEIVKASELTKAAGADFVKTSTGFAGGGATAEDVKLMKDTVGADVEVKASG
                     GVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            450..1628
                     /gene="deoB"
                     /locus_tag="RJE42_001759"
     CDS             450..1628
                     /gene="deoB"
                     /locus_tag="RJE42_001759"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV5877175.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1759..2574)
                     /gene="phnE"
                     /locus_tag="RJE42_001760"
     CDS             complement(1759..2574)
                     /gene="phnE"
                     /locus_tag="RJE42_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV5877176.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2571..3371)
                     /gene="phnE"
                     /locus_tag="RJE42_001761"
     CDS             complement(2571..3371)
                     /gene="phnE"
                     /locus_tag="RJE42_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV5877177.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3373..4146)
                     /gene="phnC"
                     /locus_tag="RJE42_001762"
     CDS             complement(3373..4146)
                     /gene="phnC"
                     /locus_tag="RJE42_001762"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV5877178.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSKIYGRTIKEDEKLGVN"
     gene            complement(4360..5316)
                     /locus_tag="RJE42_001763"
     CDS             complement(4360..5316)
                     /locus_tag="RJE42_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV5877179.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5545..7089
                     /locus_tag="RJE42_001764"
     CDS             5545..7089
                     /locus_tag="RJE42_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV5877180.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQQTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPKKFKAGSYGSEKGAYP
                     QPTTPDDHTPPNQTEKVSFDIPQNVSVNEPFEVTIHLKEFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            7140..8675
                     /locus_tag="RJE42_001765"
     CDS             7140..8675
                     /locus_tag="RJE42_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV5877181.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSGQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKDTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDPTFTIDEDDQHIRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8832..9599
                     /locus_tag="RJE42_001766"
     CDS             8832..9599
                     /locus_tag="RJE42_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877182.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNPKSKNYLEVLMAKNRDINFGTVEEDFEIGHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTELIEKVIVEREDYHID
                     EEELEVLKNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            9605..10780
                     /locus_tag="RJE42_001767"
     CDS             9605..10780
                     /locus_tag="RJE42_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV5877183.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVKIPDNIILSLINNLNDLLNGNLESLNKKESIKETAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMKKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
BASE COUNT         3900 a         1697 c         2059 g         3522 t
ORIGIN      
        1 ttcaaaatgg tgcagatgaa attgacatgg tcatcaacat cggcgcatta aaagatggac
       61 gttttgatga tgtacaacaa gacattgaag cagtggttaa agctgcgaaa ggtcacacgg
      121 taaaagtgat tattgagacg gtattgttga accatgacga aatcgtaaaa gccagtgaat
      181 taacaaaagc ggctggtgcg gacttcgtta aaacttcaac aggttttgca ggtggcggtg
      241 cgactgcaga agacgttaaa ttaatgaaag atacagtagg tgcagatgta gaagtaaaag
      301 catcaggtgg cgtacgtaat ttagaagatt tcaataaaat ggttgaagca ggtgcgacac
      361 gtattggtgc gagcgcaggc gttcaaatta tgcaaggttt agaagcagat tcagattact
      421 aatatatata aaatttggga gtgatagcta tgacaagacc atttaatcgt gtacatttaa
      481 tcgtaatgga ttcagtaggt attggtgaag cgccagacgc agcagatttt aaagatgaag
      541 gttcacatac tttaagacat accttagaag gcttcgatca aacattacca aaccttgaaa
      601 agttaggtct agggaacatc gataaattac cagtagtaaa tgcagttgaa caaccagaag
      661 catactatac taaattgagt gaagcttcag ttggtaaaga tacaatgact ggtcactggg
      721 aaattatggg attaaatatt atgcagcctt ttaaagtata ccctaatgga ttccctgaag
      781 agttaattca acaaattgaa gaaatgacag gtcgtaaagt tgttgctaac aaaccggcat
      841 cgggtacgca aattatcgat gagtggggcg agcaccaaat gaaaactggt gacttaattg
      901 tttatacaag tgcagaccca gtattgcaaa ttgctgcaca tgaagacatt atcccattag
      961 aagagttata tgatatttgt gaaaaggttc gtgagttgac aaaagaccct aaatatttaa
     1021 ttggtcgtat tatcgcacgt ccatatgttg gtgaaccagg aaactttaca cgtacatcta
     1081 atcgacatga ttatgcgtta aaaccatttg gtaaaactgt cttagatcat ttgaaagacg
     1141 gtggttatga tgtcattgcc atcggtaaaa ttaatgacat ttatgatggt gaaggtgtaa
     1201 cagaagcggt tcgtacgaag agtaacatgg acggtatgga tcaattgatg aaaattgtta
     1261 agaaagattt cacaggtatt agtttcttaa acttagtaga ctttgatgca ttatacggtc
     1321 atcgtcgtga taaaccaggt tatgcacaag caattaaaga tttcgatgat cgcttgccag
     1381 aactgtttag caacttaaaa gaagacgatt tagtaattat tacagcagac catggtaatg
     1441 acccgacagc gccaggtacg gaccatacaa gagaatatat cccagtaatt atgtacagtc
     1501 cgaaatttaa aggtggtcat gcactagaaa gtgatactac attcagttct atcggtgcaa
     1561 ctatagcgga taatttcaac gtaacattac cagagttcgg taaaagttat ttaaaggaat
     1621 tgaaatagaa taaatttaga tattataaaa atagcagtga agttaactat aataatagtt
     1681 ttcttcactg ctgtttttat tataatagag aaacgtaaga cggtagtacc tcttatttag
     1741 gagtatcctg atttaatgtt aaacaatacg ttttcggatt gaaccggaaa ttaaatcgac
     1801 aattgcgacc attagtacta aaccgattaa tataatacct acacggtccc aagaacgtgt
     1861 ttgaatggca aatatgagtg gtgtcccgat accaccagct ccaattagcc ctagtataga
     1921 agctgaacgt aagtttagtt caaagcgata aagtatgagt gatagaaagg caggcataat
     1981 ttgtggtatg actgcaaata cgagtgtttt aatcttattc gcaccactgg cctttaatga
     2041 ttctacagca ctgaaatcta gaccttcaat atcttcagct aaaagtttcc caagcatacc
     2101 tacggaatgg atacctaaag ctaatacacc tgaaaatgaa cctgggccaa cagctttgat
     2161 aaatataagt gccattacaa tttctgggaa gacacgtata acacttaaaa taaatttgct
     2221 aacacctgaa actgggcgta gctttaccat attatttgca cctagaaatg ctaatggaat
     2281 acagataatt gcggcgatga aagtacctac aacggctatc gcaaaggttt caagtaaacc
     2341 acgtaataag tcttcgccat ctggtatata gatatagctg atatcaggat ggaataatcc
     2401 gctgaatatg gattttaaga tttctaatga tttactttta agttctaaac ttggtacacc
     2461 tgcaaatgcc cagatgataa tagctaagac gacaattgca ataagccatc ttttaatcaa
     2521 ttttcgtttg tgtgcttttg tgtgaacatt atattttgct atttcctgtg tcatgcgaga
     2581 tgtgccctca ctttcgtact gatgtaatca atgacgacga cgataactaa agtaaataaa
     2641 ataatcgttg ctgtttttgg atattgaaat aaaccaagtg tttgatcata aaacaatcca
     2701 ataccgccag cgccgactaa tccaagcaca gctgaagcac gtatatttac ttcaaatgca
     2761 tataatacgt atgacataaa tgacgatatg gcttgtggta caacaccgaa aacaatccat
     2821 tttattttat tagcgccaac agccgtcatt gcttccattg gacctggatc tatcgtttcc
     2881 aatgattcat ataataattt tccaataata cagatagtta aaataaacaa tgctaatatc
     2941 cctggaattt gaccgattcc aaatacagcc acaaagattg ctgctaatag caaatctgga
     3001 atagtacgaa ctatatttaa aataaagcgc gagggtattg aaatccactt ttgatgaacg
     3061 atattgctag cacataataa cgcaattggt attgaaacga tgctacctaa tactgtactt
     3121 acgatagcca ttcgaatggt atctaacatt ggcgttgtaa tttgttgtaa atactcgaaa
     3181 tcaggtggaa tcatttgttt gaatagatca cctatttgag gtattcctat cattaaatct
     3241 ccaaaattaa accccgtata aatgaagctc caaatgataa gcacaatgat taacatgaag
     3301 gtaaaactcg tttttaaaga aacctttttc tttaaaaggg agtcatactt tgtaggtgtt
     3361 tctaaaggca tgttagttca ctcctagctt ttcatcttct ttaattgtac gtccatatat
     3421 tttactaaat acgtcatctg ttgcttcaga tgcaggacca tcatagacaa cttcaccatc
     3481 acgtaaacca atgatgcgtg tgccatattc ttttgctaag tcaacaaaat gtaagttaat
     3541 taaaattgtg atgcctaatt cttggttgat ttttcttaaa tcatccataa cctgtttcgt
     3601 agttaatggg tctaatgaag caactggctc atctgcaaga ataatttcag attcttggca
     3661 tagcgcacgt gcaatagata tacgttgttg ttggccacct gataattcat cagagcgttg
     3721 attatattta tctaagatgt tgacccgttc tagtgcatcc attgctttaa ttttgtcttc
     3781 ttttggaaat aaacctaata ccattttcca agtagggtga taacctacac gtccacttag
     3841 tacatttcgt aatacacttg accgtttaac taaattaaaa tgttggaaaa tcatacctat
     3901 atttcggcgc atttctaata atgctttacc atgggcttta gtgattgatt taccttggat
     3961 gaaaatttca cctgacgtga tatcatgcaa acgatttaca gatcttaata acgtggattt
     4021 cccagcacca gatagtccga caatgactgc aaattcacct ttttcaatat ttaagttaat
     4081 atttttcaag cctacatgac cgttaggata gactttactg acgtttttaa attcgatttg
     4141 actcatgttt gacacctttc tttaataaag aaaagccaag gtaaactgaa atatcataga
     4201 cagtgggttt ctgtgtacaa atagtttcaa atgtacagcg acacctttct atatctgttt
     4261 cagtttagcg cctagctttt caatattaat ctagaatata tctattgaac gaaagctttt
     4321 aataccaaat tcgataatga ttcattggtt aaataatgat tatttcatat ctttaactaa
     4381 tttttcgtac tctcttacaa tgtcgaaatt tgaatctttc gtttctgtgt atccttcatg
     4441 tgaataaact tcgctaataa ttttgtgacc ttcttttgat ttagcaatgt ctataaaagc
     4501 ttttttcaat ttctcttgga agtctttatc catatctggt cttacagaaa ttgtgtcatt
     4561 cggaatagct tgtgttaatt ttaaaattcg tgtgtcttta aatacatttg gttggtcttt
     4621 tttcacagta ttacgtgcat cgttaaatac agctgcagca tctacatctc catttaataa
     4681 tgagataact gcttggtcat gacctttaac attcacaatt ttcatatctt tagttgcatt
     4741 aatacctgct tcgtttttta acatcgcaag tgggaatgta tatccagcag ttgatgttac
     4801 atcttgtaag gcaattttct taccttttaa atctttcaag cttttaattt ttgagtcttt
     4861 tttaacaaga atttctgatt tataactatc tacaagttct ttacttgctg aaccatcttc
     4921 ttttacacca aaacgttgtg cttgtaataa taaatcagct gctttttgat catgtgctaa
     4981 tgtgtatgcc gttggtggta agaaaccaac atcaactttt ttagacttca tagcttcaac
     5041 aattgtattg tagttagttg atacagacac tttaactgga atccctaatt ctttagatag
     5101 taatttttct aatggttttg ctttagcctc taatgttcca gcattttgcg aaggtacaaa
     5161 ttgaactgtt aattctttag gtttgtatcc tcctgattta gaatccgaat cattactagc
     5221 gttcttttga ttatctaaag aacttgaatt tccgcatgct gctgcaaaaa caatgactgc
     5281 taacattaat acaaataaac acttaaaatt tttcatttga taactgtccc cttcacattc
     5341 ttaatcgtgt aatatgtatg caatgaaatt acacttatag cgtaccacat ttaaatacga
     5401 aaaagtattt tataaaaatg aattttaata actttcttta agagtttgat aagattttgt
     5461 aaaattttcg ttaaattgct catttcaaac atatatttat cctataattc aagaacaaat
     5521 ttattttaaa aggagcctca caaaatgaaa aaaatatata agtcattaac tgtctctgca
     5581 attgttgcaa cggtatcatt aagtgcttta ccgcaatctt tagctataac gcatgaatcg
     5641 caacctacaa agcaacagca aacagtatta ttcgatcgtt cgcatggtca aacagctggt
     5701 gctgcagatt gggttagtga tggtgcattt tcagattatg cggattcaat acaaaaacaa
     5761 ggttatgacg ttaaagctat tgatggtcat tcgaacataa cagaagcaag tttgaaaagt
     5821 tccaaaatat ttgtaattcc tgaggctaac attcctttca aagaatcaga acaggcagca
     5881 attgttaact atgtgaaaca aggggggaat gttgtcttta tttcagacca ttacaatgct
     5941 gaccgaaatt taaatcgtat tgattcatca gaggcaatga atggttatcg acgtggagcg
     6001 tatgaagata tgtcgaaagg tatgaatgca gaagaaaaaa gttctactgc aatgcaaggt
     6061 gtgaaaagtt cagattggtt atctacaaac tttggcgtac gttttcgata taatgcacta
     6121 ggtgatttaa atacgagcaa tattgtttct tcaaaagaaa gtttcggtat tactgaaggt
     6181 gtgaaatctg tatctatgca tgccggatcg acattagcaa ttactaatcc agagaaagca
     6241 aaaggtattg tgtatacacc agaacaattg ccagcgaaaa gtaaatggtc acatgctgta
     6301 gatcaaggta tttataatgg gggcggtaaa gcagaaggtc cctatgtagc aatttctaaa
     6361 gttggaaaag gtaaagcagc atttatcggt gattcatcac ttgtggaaga tagttcgccc
     6421 aaatatgtaa gagaagataa tggagaaaag aagaaaacat atgatggttt taaagaacaa
     6481 gacaatggta agctattaaa taatataacg gcttggatgt ctaaagataa tgatgggaaa
     6541 tcacttaagg cgagtggcct aacattagat acaaagacta agttgcttga ttttgaacga
     6601 ccagagcgtt caactgagcc tgaaaaagag ccatggtcac aaccgcctag tggttataaa
     6661 tggtatgacc caaaaaaatt taaagcaggt agttatggca gtgaaaaagg cgcatatcct
     6721 cagccaacca caccagatga tcatacgcca ccaaatcaga ccgaaaaagt atcatttgat
     6781 atcccgcaaa atgtttctgt aaatgagcca tttgaagtga caatacattt aaaagaattt
     6841 gaagcaaatc aaacacttga aaatcttaga gttggtattt acaaagaagg cggacgtcaa
     6901 atcggacaat tttcaagtaa agataacgat tataacccgc caggttacag tactttgcca
     6961 acagttaaag cagatgaaaa cggaaatgcc acaattaagg tcaatgctaa agtacttgaa
     7021 agtatggaag gttcaaagat tcgtttaaaa ctcggtgaca aaaccttgat tacaacagac
     7081 ttcaaataaa tatataatag aaataagaaa gatgtttgtg attgaaggag tgagtgagga
     7141 tgtcaaacat agcattttat gtcgtgagtg acgtacatgg ttatattttc ccaacagatt
     7201 ttacgagtag aaatcaatat cagcctatgg gattgttact agcgaatcat gttatagaac
     7261 aagacagaag gcagtatgac caaagtttta aaatagataa tggtgatttt ttgcaagggt
     7321 caccattttg taattactta atcgcgcata gcggcagtgg ccagccttta gttgattttt
     7381 ataatcgaat ggcattcgac tttggtacgc ttggtaatca tgaatttaat tatggattac
     7441 catacttaaa agacacttta cgcagactca attatccagt tttgtgcgct aatatttatg
     7501 aaaatgatag tacattgact gataacggtg tgcagtattt tcaggttgga gatcaaacgg
     7561 ttggtgtgat aggtttaacg acacaattta ttccccattg ggaacaacca gagcatattc
     7621 agtcacttac gtttcatagt gcttttgaaa cacttcaaca acacttgcct gaaatgaagc
     7681 gacatgcaga tatcattgtg gtttgttacc atggtggatt tgaaaaggat ttagaaagtg
     7741 gtacgccgac cgaagtgtta acgggtgaaa atgaaggata tgccatgtta gaagcatttt
     7801 ctaaagatat agatatcttt attacgggtc accaacatcg acaaattgct gaaaggttta
     7861 aggatacggc tgtgattcaa cctggtacga gaggtacaac tgtaggcaaa gttgtcttga
     7921 gtactgatga atatgaaaat gtatccgttg aatcatgtga attacttcct gttatagatg
     7981 atcccacatt tactattgat gaagatgacc aacatatacg aaagcagtta gaggactggt
     8041 tagattacga aattactaca ttgccatatg atatgacgat taatcatgca tttgaggcac
     8101 gtgtggcacc gcatcctttt actaatttta tgaattacgc tttattagaa aaaagtggag
     8161 cagatgttgc ctgtacagct ttgtttgatt ctgctagtgg tttcaagcaa gtcgtgacga
     8221 tgcgagatgt tattaacaat tacccatttc caaatacatt taaagtttta gctgtaagtg
     8281 gtgccaaact taaagaagcc attgaacgat cagcagaata ttttgacgtg aaaaatgatg
     8341 aagtaagtgt gagcgcagac ttccttgaac ccaaaccaca acactttaat tatgatatat
     8401 atggtggcgt aagttatacc attcatgttg gaagaccaaa gggacaacgt gtgagcaata
     8461 tgatgattca aggtcacgca gttgatttaa agcagacata tacaatttgt gtaaataatt
     8521 atcgtgcagt tggcggtggt cagtatgata tgtatatcga cgcgccagtt gtaaaagata
     8581 ttcaagttga aggcgcacaa ctacttattg attttttatc aaataataat ttgatgcgca
     8641 tcccgcaagt tgttgatttt aaagttgaaa agtgacggat atatgtaaaa cttacttgat
     8701 tttaatcggg taagtttttg tttttgttta aactaatcac tcttaaaaac caatatactt
     8761 taagaaaaat gttgatttta caaaaaataa ggcttatatt ttatataact taatattagg
     8821 aagtgattaa tttggaaaca attaaacaag aagtaagaga atctattgtt aaaggaatta
     8881 tcgcgggata taaggactat gttaatgaga gaaatgaagt caagaaacgt atggtgatta
     8941 gtgatgccta tgtgtacacc aaatctaatc atattgaaaa tcaagttgct aagcatttgg
     9001 aaaattttgt taagtatatt aaagaaaatg ctggaccatc atggaagtat ttaaagttta
     9061 tatttaataa agatgaagag aaaaataata tcatgtttat attgaaaaat gaagattact
     9121 ttgatgaaaa aaatgtaagc gtagggaaga gtttagttgc tgataaaaat ccaaaaagta
     9181 aaaactacct agaggtactt atggctaaga atagggacat taattttggt actgttgaag
     9241 aagattttga aattgggcat caaatgactg cagatagtat attatttaat ataaaagaag
     9301 gcagtaatag agatattaat tcttattttt taattattac atatcgtatt gataaagaat
     9361 caaagcaatt agctgcaatc aaacaatggt taccaaatcc agaaacaaat tctgcaatta
     9421 tggttgatga tttgacagag ttaatagaaa aagtaatagt agaaagagaa gattatcata
     9481 ttgacgaaga agagttagaa gtacttaaaa acgatggaga gttagaactc atagatgtgg
     9541 aacatgcatt tggtatcagt atcgatgaag gtaacgatat gattgaaagt gaaaggtgaa
     9601 gttaatgttt attggcaaga atttagaata cgtgagaaag cttaatgctc taagtagaaa
     9661 agaattatca gaaaaaataa atgtgagtga acaagcaatt tggcaatatg aaacaaaaaa
     9721 tatgatgcca gaaattagta aaatttatga tatgacatct atttttaatg ttaaatctag
     9781 ctactttatt agcgagcagc ccgaagagct cctaataaat tctgtcgata aacatagtat
     9841 agcttttaga gcaaaaaatt ataaagtatc tacgaaacta ttaaataagc aatattatca
     9901 agcaatgtat ttatctaatt taactagcta cttattttct tttgtaaaga taccggataa
     9961 tattatttta tctttaataa ataatttaaa tgacttgttg aatggcaatt tagaatcact
    10021 aaataaaaaa gaaagcataa aagaaactgc taaagttgtg agggctaaaa ttcttcaaga
    10081 cgaaagcaat gaagcactat tatttatgct tgaaaaagcg ggtatagtta tatacgaaaa
    10141 gcgaattaat gatagcatag acgcatatag tttttggagt aaagatcaga caccttttat
    10201 tatactgggt acaaataagg gggtagcagt tagaaggaat tttgatcttg cacatgagct
    10261 gggacatctc gttctacatc gtcatattca attcgactta ttatctcctg aagaatataa
    10321 gacaattgaa catgaggcag atattttcgc ctctgaattt ttactacctg aagaagcgtt
    10381 taaaaaagac tttgatcaaa tgaaaaaaaa atcaaatcct gactatttag ctgtattgaa
    10441 agaaaaatgg tatgtttcta tacaagcaat tgcaatgaga gcctattatc taggtctaat
    10501 gtctagtact cagtacagat atttttgggc atcattaaat aaaaaaggct ataaaagtaa
    10561 agagccttta gacgacgtga ttgaaatgtc acgtcctgtc aaaatgaata gtttattaaa
    10621 gttgtatttt gatagaaata tactgacacc acaaaaatta ttgaattatt taaaagttga
    10681 tgaaacattt ttgaatcatc tagcaggtat taatttaaaa ctctttaagg attatgttaa
    10741 tgaaaataga gaatataata taacgaatct atataaataa ggcaccaata tttaaacctc
    10801 gagctgaaaa gatcatcatt tcagttcgag gttttttatt tgtaacatct gttgataggt
    10861 atcgacaagg accttttaat gttgtgtttt gtgcgatata acaagctttt taggctatta
    10921 aagaatattc agaattaaat atataattat gaataaatta tgtcattgaa aaaattatga
    10981 ttataaaatc attctaaaaa caccggaata acaatgattt catgaaaaca tttattttaa
    11041 aatttgatat ttgttcaaat aatattcgaa attaaacttt tttgtataga attttcttta
    11101 tatcctgaga gacatgtact ataatgtttg tgaaataatt cacaaagtat aaaggagtgg
    11161 ttgtatatgt taactata
//
LOCUS       DAPIDM010000079        10943 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000079 DAPIDM010000000
VERSION     DAPIDM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.079"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..140)
                     /locus_tag="RJE42_001768"
     CDS             complement(<1..140)
                     /locus_tag="RJE42_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDV5877184.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GI"
     gene            complement(159..1421)
                     /gene="femA"
                     /locus_tag="RJE42_001769"
     CDS             complement(159..1421)
                     /gene="femA"
                     /locus_tag="RJE42_001769"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDV5877185.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            complement(1745..2473)
                     /locus_tag="RJE42_001770"
     CDS             complement(1745..2473)
                     /locus_tag="RJE42_001770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV5877186.1"
                     /translation="MTKLFIPYIMGNKDLIENAKILSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDFIFEQLEKHGNQIKCQYVLMTYYNIICHYGEQAFFERCRDS
                     GVYGLIIPDLPYELSQRLKQQFSHYDVKIISLVAMTTDDERIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKEIANVPVVAGFGIRTPQHVADIKAVADGIVIGSEIV
                     KRFKANTREEIIEYLQSIKHALNN"
     gene            complement(2466..3680)
                     /gene="trpB"
                     /locus_tag="RJE42_001771"
     CDS             complement(2466..3680)
                     /gene="trpB"
                     /locus_tag="RJE42_001771"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV5877187.1"
                     /translation="MNKQIQTEVDELGFFGEYGGQYVPETLMPAIIELKRAYKKAKED
                     PEFQRELEYYLSEYVGRETPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGLPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            complement(3673..4305)
                     /locus_tag="RJE42_001772"
     CDS             complement(3673..4305)
                     /locus_tag="RJE42_001772"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDV5877188.1"
                     /translation="MKLKFCGFTSIKDVTAANQLPIDAIGFIHYEKSKRHQTIAQIKK
                     LASAVPEHIDTVCVMVNPDLTTIEHVLSNTPINTIQLHGTETVDLIKGIKKKYPRIKI
                     IKALPADEHIIQNINNYKAFFDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIRTVNQLKLSHLGYDLASGIEVDGRKDIEKMTAIVNIVKGERENE"
     gene            complement(4305..5087)
                     /gene="trpC"
                     /locus_tag="RJE42_001773"
     CDS             complement(4305..5087)
                     /gene="trpC"
                     /locus_tag="RJE42_001773"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDV5877189.1"
                     /translation="MTILEEIVEYKQSLLQNGYYQDKLSTLKSVNIQNKKSFINTIEQ
                     EPKLVIIAEIKSKSPTVNDLPERDLSQQISDYEQYGANAVSILTDEKYFGGSFERLQE
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLRELYEYATSQSLEV
                     LVEVHDREELDRAYKINPKLIGVNNRDLKRFVTKVEHTNIILENKQPNHYYISESGIH
                     DTSDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            complement(5089..6087)
                     /gene="trpD"
                     /locus_tag="RJE42_001774"
     CDS             complement(5089..6087)
                     /gene="trpD"
                     /locus_tag="RJE42_001774"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDV5877190.1"
                     /translation="MSLLTRIKTESILHESDIKELIEILISPSIGTDIKYELLSNYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGSMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNRSITSNSGSTDLLNQMRISTTSVDDTPKQLDEKNLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLSYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKYAPNSDFKGGLPEENLAISLNI
                     LNGKDQSSRRDVVVLNAGLSLYVAEKVDTIAEGIELASTLIDNGVALKKYHQMRGE"
     gene            complement(6090..6659)
                     /locus_tag="RJE42_001775"
     CDS             complement(6090..6659)
                     /locus_tag="RJE42_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDV5877191.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISSYQDKPILGICLGAQALTCYYGGEVIKGNKVMHGKVDTF
                     NIKTENNSLLYEGVPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKILMNFINSVKGGR"
     gene            complement(6656..8062)
                     /locus_tag="RJE42_001776"
     CDS             complement(6656..8062)
                     /locus_tag="RJE42_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV5877192.1"
                     /translation="MDVFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSIVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKPLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHANQHLNQLSFQLYQNLKRQNPSPYMYYLNIDKPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGEINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            complement(8555..9586)
                     /locus_tag="RJE42_001777"
     CDS             complement(8555..9586)
                     /locus_tag="RJE42_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDV5877193.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILKLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9751..10842
                     /locus_tag="RJE42_001778"
     CDS             9751..10842
                     /locus_tag="RJE42_001778"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDV5877194.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIHMSTKPGVIVSDTGSTKAMI
                     QQHECSLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNSQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAEA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
BASE COUNT         3329 a         2009 c         1606 g         3999 t
ORIGIN      
        1 ttaataccta ataaaactac ttcaaagcca tcattctcac gggtaactat attttctttt
       61 acttggaaat aatgactttc caatgatgga ttttgtacaa agttgtcaaa ttcggtaaca
      121 gttaactctg taaatttcat gttttgataa ttcccttcct aaaaaattct gtctttaact
      181 tttttaagtg cggtatatgc tgcgtaaaca ggtttattaa ttggtttaat aaagtcacca
      241 acatattcaa taatttcagc attgtaacct tttttgaatt taactacacc agcatcttca
      301 gcatcttctg taaatttacc actaacacca tagaaattat aacggtcaat gccatgattt
      361 aatgcataat taatcatttc ccattgcact gcataacttc cggcaaaatg gcggaatgca
      421 tttgatgtac caccagcata ataaacaact tcaaatggat tgataaagaa gaaaccagca
      481 gagataggta attcattacc atgttcttct tgtagacgtt taccttcttc aatcttttgc
      541 tcatttgcat caagttgttg ttgtaagtta tctcgcttgt tatgcgcttt tttgttttca
      601 ggacgttttt caatatcctt taatgcttta tttaaatctt tatttaaaat atcacgctct
      661 tcatttagtt ctttaatata ttcatcaaaa ttgatatacg ctaaaggcac taacacacgg
      721 tctttgtaat attttaagcg attgtaataa aacttgtcat cacgatcagc aaaagctttt
      781 gattctgacg tatcttccat aaatgatcta aaaattggta gttcttcttc agataaatat
      841 cttactttaa caccattctt tttaactttt ttcgtgtttc tttttctaag tccatccata
      901 tttttaatga tgtcatctgc tgttttatct tttaaatcta acactgagtg ataacgaatt
      961 tgtagcacag gatcaaaccc tttatggaat ccagtatgtt caaatcctaa gttactcatt
     1021 ttatcaaaga accaatcatt accagcatta cctgtaatct cgccatcatg attcaagtat
     1081 tgatatggta aatatggatc gatatgtagg tatagacaac gatgtttttt aacatatttt
     1141 gataattcat taaagaaaaa gtgtacgagt tcttgatttt cataatcgat tactggaccg
     1201 cgatttgaat aaaaatactt gaacactttc ataacaggta cagcagtaag taagcaagct
     1261 gcaatgacct cgttattatt gttttttatt cccactaaat gtgtttcata accttcagca
     1321 agctttaact catagtggcc aacagtttgc gtgaaatgac tgtatggcat gctatccgta
     1381 aaggcaccaa actctttagc tgttaaattt gtaaacttca ttatcattac tcctatttgt
     1441 ctctcgttaa ttaatttcat ttccgtattt gcagtttttc tatttcccct ctgcaaatgt
     1501 caaaaataat aaatctaatc taaataagta tacaatagtt aatgttaaaa ctaaaacata
     1561 aacgctttaa ttgcgtatac ttttatagta atatttagat ttttgcatac aatttcaaaa
     1621 aaagtaatat gaacgtttgg atttgcccat attacttttg aatagttgct attcagtttt
     1681 ataatttacc gttttatatg acttttaaac agcattaagc tattttatat taaatcaatg
     1741 aatcttaatt gttcaaggcg tgtttgatag attgtaaata ttcaatgatt tcttcacgcg
     1801 tgttagcttt aaatcgctta acgatttcac taccaataac aataccatct gcgaccgctt
     1861 ttatatccgc aacatgctgt ggggtcctta taccaaatcc tgcgacaact ggcacattgg
     1921 ctatctcttt aattgattcg atttttcgtt ttaattctgg atgaaaggca ccgttttgtc
     1981 ctgtggtcgc attcatcgtc acagtataaa taaatccttc cgcatgagat acgatatctt
     2041 ttatacgttc gtcatctgta gtcatagcaa ctaacgatat gattttgaca tcatagtgac
     2101 taaattgttg ttttaaacgc tgtgataatt cgtatggtaa atcaggaatg attaaaccat
     2161 aaactcctga atctcggcat ctttcaaaaa atgcttgttc accgtaatga caaataatat
     2221 tataatacgt cattaataca tactgacact taatttgatt accatgtttt tctaattgtt
     2281 caaaaataaa atctatcgtg atgccttgtt taatcgcttg ttgtcctgct tccatgataa
     2341 ctggaccatc agcaacggga tctgagaatg gcacacctat ttcaataata tcggcaccat
     2401 tttcgcttaa tatttttgca ttttcaatca aatctttatt gcccataata taaggtatga
     2461 ataatttagt catttgcaag acctcgctct gccatatatt gtctaattgt ttccatatct
     2521 ttatcgccac gtccagaaat agttactaca ataatatctt ctttcgacat cgtaggcgct
     2581 agtctttcaa cataactcag tgcatgtgca ctttcaattg caggtataat accttcatgt
     2641 tttgtaaagt tgattaaagc attcattgct tgtgtatcac ttgcattttc aaaagttact
     2701 ctaccaatgt cgtggtaata agaatgttct ggcccaatac caggataatc aagtcctgct
     2761 gaaatagaat gtgctagttg cacttgcccg ccttcatctt gaattaaata cattttagta
     2821 ccatgtaata cgccaggtaa tcctttgcca attgcaagtg catgtttatc agtatcctcg
     2881 ccttgacctg cggcttcaac accgtataac gcaacatcat ctttaataaa tggataaaat
     2941 gtaccgattg catttgagcc accaccgata catgctacaa ttgcatccgg aagtcgacct
     3001 tctttcttca atatctgtga ttttatttct ttaccaatca cactttgaaa atctctaaca
     3061 atcgttggga atgggtctgg acctaatgca gaacctaata aataatgtgt atcatctaca
     3121 tgacttaccc aatattgcaa tgctttattc actgcatctg ataatgtccc ttgaccatct
     3181 tcaacagcaa caacttttgc accaagtaat tccattctaa atacattgag ttgttgtcgt
     3241 ttaatatctt cgcttcccat aaagacaaca agttccatat caaataatgc agcaacagta
     3301 gcactagcta caccatgttg acctgcacca gtttcagcaa caagcttctt cttgcccata
     3361 cgtttcgcaa gtaatgcttg tcccaatgca ttattaattt tatgtgcgcc tgtatgattt
     3421 aaatcctcac gtttcaaata tattttagct ccacctaagc tttcagtata tgatgcagca
     3481 taagtaagtg gtgtctcgcg ccctacatac tctgataaat aatattccag ttccctttga
     3541 aactctggat cttcttttgc ttttttataa gcccttttta attcaataat tgctggcatt
     3601 aacgtttcag gtacgtattg ccctccatat tcgccaaaga aacctaattc atctacttct
     3661 gtttgtattt gtttattcat tttccctttc tcctttcaca atatttacaa ttgctgtcat
     3721 tttttctata tcttttcgtc catctacttc aatacctgat gcgaggtcat aacctaaatg
     3781 tgataattta agttgattaa ctgttcgaat gttttcagag ttaattccac ctgcaattaa
     3841 ataaggtatg tctttgatgt gcttcaaaat agtccaatca tatgtttgac ccgtacctcc
     3901 ataagacact gaaggtgtgt cgataataaa taaatcaaag aatgctttat agttattaat
     3961 attttgaatt atatgttcat ctgcaggtaa agctttaatg attttaatcc ttggatactt
     4021 ctttttaatt cccttaatta aatctacagt ttctgtgcca tgtaactgta ttgtgttaat
     4081 tggcgtatta cttaatacgt gttcaattgt tgttaaatca ggatttacca tgacgcatac
     4141 tgtatcaata tgttctggaa cggcagacgc taactttttt atttgggcaa ttgtttgatg
     4201 ccttttactt ttttcataat ggatgaaacc tatcgcatca ataggtaatt gattggccgc
     4261 tgtaacatcc ttaattgatg taaagccaca aaatttcaat ttcatcatga cttcaccttt
     4321 tgcattttta gttgtggtaa aaattcagat agattgtcgc aacgcataag cgcctcacct
     4381 attagtaagc catcgatacc actatgcaag atttttctta catcagaggt atcatgaata
     4441 ccgctttctg aaatataata atgattcggc tgtttatttt ctaaaataat atttgtatgt
     4501 tccacttttg taacaaaacg ttttaaatca cgattattta caccgattaa tttaggatta
     4561 atcttatagg cacggtctaa ttcttcacga tcatgaactt ccaccaatac ttctaaactt
     4621 tgagaagtag cgtattcgta taactctctc aattgtttat cagataagat attaactata
     4681 agtaaaatca tagatgcacc agcttgttta gcaacgtcaa tttgaagcgg atctataata
     4741 aagtctttac acaatactgg aagtgtcgtt tttgttgtta attcttgtaa acgttcaaag
     4801 ctccctccaa agtacttttc atctgttaaa attgaaactg catttgcacc gtactgttca
     4861 taatcagaaa tttgttgaga taaatctcgt tcaggtaagt cattaactgt gggactcttt
     4921 gatttaattt ctgcaataat tactaacttt ggttcctgtt caattgtgtt tataaaagat
     4981 tttttattct gaatgttcac actttttaaa gtgctaagtt tgtcttggta atagccattt
     5041 tgtaaaagtg actgtttata ttcaacaatt tcttctaaaa ttgtcatgtt attcacctct
     5101 catttgatgg tatttcttca aagctacacc attatcaatc aaagtgcttg cgagttctat
     5161 gccttctgcg atggtatcca ctttctctgc aacatatagg cttaaacctg catttagtac
     5221 gacaacatca cgacgacttg actgatcttt accattcaag atattaagtg agattgctaa
     5281 attttcttca ggtaaaccgc ctttaaaatc gctattcggc gcatatttca aaccataatc
     5341 agtcgcattt aatgtgtagt ttttgatttc tccatcttca gtcaattcat atattaaatt
     5401 atcgccagac aatgttgctt catccatccc atttgcgcca tgtaaaacga ttgcacgttt
     5461 tctacctaaa tctttaatcg ttttagcaac tagctttaat tttgtaggat caaagacgcc
     5521 taccatttga tacgataaat gatatggatt aattaatgga ccaacaagat ttaaaatagt
     5581 aggttttcca atcatttttc taactggttg catatatttc ataatggggt atgattccgt
     5641 tgcaccaatg aatactagat ttttttcatc taactgcttt ggcgtatcgt ccacactagt
     5701 tgttgaaatt cgcatttgat tcagcaaatc cgtactaccg gaatttgagg taatacttct
     5761 atttccatgt ttaatgactt tcacaccagc gctcgccaca acgaatgcaa cggttgtcga
     5821 gatattaaaa ctatttgatt tatctccacc tgtaccacaa acacacatac taccttcata
     5881 acaaggttga tgtggataca tcgtattaat taaacttctt acgatgtatg ttaattcttg
     5941 ttgttgaatt tctcgttctg aataattact taataattca tattttatat ccgttccaat
     6001 actaggtgaa ataagtattt caattagctc cttaatgtca ctttcgtgta aaatcgattc
     6061 agtttttatt cttgttagta acgacataat catcgccctc cttttacaga attaataaaa
     6121 ttcattaata ttttcacgcc gtaatctgta gcaaatgatt caggatggta ctgaataccg
     6181 taatgcggtc tttctttatg ctcaaatgac tgtatacaat cttcggtacg tccagtaatt
     6241 ttcaattctt ctggaaaatt atcaggatta cttattaatg aatggtatct cataatcgaa
     6301 aattgttctg gtacaccctc gtataacaaa ctattatttt cagttttaat attaaaagta
     6361 tcaacttttc cgtgcataac cttattacct ttaatgactt ctccgccgta ataacatgta
     6421 agtgcttgag cacctaggca aatacctaaa atgggtttat cttgatagga tgagataatt
     6481 ttcattaact gttggtcgtc taatggatgc ccagggcctg gtgaaataat gacagcatca
     6541 acagattgat tcagcacatt atcatcatca gggtattgaa caatgacgtc agtatgttga
     6601 gcaacaatat ccactaggtt atacgtaaag gaatcataat tatctacaac taagatcatg
     6661 ggctcacctc caataagctt ttagctttca atttcgtttc attcagttct ttttcaggaa
     6721 tagaatcata tacaacgcca caaccagctt ctacgttgat atactgctca tctatcatca
     6781 tcgttcgaat tgctaatgca aaatctaagt tatgattaca atttatgtat ccaacaccac
     6841 cactataaac gccacgttta tgtggatatt gttcgtaaat tctttcgatt gcacgtaatt
     6901 ttggtgcacc tgaaacggta cctgttggta ataaattcgc aataactgtc attggcgata
     6961 aattttgatt tatttcacct gtgacttcac ttacgatatg cataacatgt tcatattttt
     7021 caataaccat taatttagta atttttgagg tacctatttt acttactcta tgaatatcat
     7081 tacgccctaa atcaacaagc atacgatgtt cactgcattc tttaggatca ttaagtagtt
     7141 gtttcatatt ctcattatct atttgtgtcg tctcaccacg ttgaatcgta cctgcaatag
     7201 gattagttgt tacaatttga tctttgacac ttacgaaact ttcgggagaa ctaccgacaa
     7261 tgtagggttt atcgatatta agataataca tatatggact tgggttttgt cgttttaaat
     7321 tttgatacag ttgaaacgaa agttgattta aatgctgatt ggcatggtgc gcatatttgt
     7381 aaattcttga tggcacaact tggaacatat ccccttctgt tattttctct ttgaaatact
     7441 gaatcatttc gataaatctt tcttcagaaa tgtttgattg aatttcttta gttttaaaat
     7501 caaaatcttg tgtaggcatg aatggttgga ttttagttaa gtcttcgata gacttattaa
     7561 ctcgattctc aagatctgat tttgttgaat ttgaaaattg attcgtcgcg atgatatata
     7621 actcatcttt gtaatggtca aatacataaa cttgttcaac catatataac cgtacatcgt
     7681 gttgcttgtg atcctctaat tgtattgatt gcaatttagg aaattcatgt cttactaaat
     7741 caaagctaca cgtcccaaca tatccagata taaacggtaa tggcttaagt tgctcatctt
     7801 gaatattatg gtagtcttca tttattttag tcgttaaata atgatacggt ctttcagtga
     7861 tttgatacga ctcttttaaa gtgcttactg ataatacatc gttatctaaa gttaaagtac
     7921 cgtagatatc aaatatcact atagaatagc gacccttagt ttgttgttga tttgtacttt
     7981 ccaaaatgat cttcttggaa tgaagttgtg ctaaaacttc aggtgttaca tttgctttta
     8041 tttttttata gaatacatcc attctttacg ctcctttcta aaaaaacgcc agcatcctta
     8101 tcaatataaa ggacgctgac gcgtggtacc acctaaatta accgaatatt cgaaatattc
     8161 atagttcact ctttactata tataattttt ctgttgcaga caaaacccaa tttcattaat
     8221 ctcggtgtta gttcacatca cccactaact ttctgttgct acctttaact aactactaca
     8281 ttttgtctaa ataaaaagac accacataac gcatatacct gtaaggacgc gttaccgtgg
     8341 tgccacctta gttaacataa ttcatgttca ctttcaaaac tcattcaatt gttttccaaa
     8401 gtccaagtca caagatatgg cgtgctaatt cccatcaccc attagctttc tatttgctac
     8461 gtttcatatc tgctactatt tctttagaaa attctaatta tttttatagt tcattatatt
     8521 acataggtcg ttcacatttg tcaacactaa ttgtttactc aattggtgat aagcaatatg
     8581 catatagtaa tttctctgtc gctttaatag aatcaatatg tgttcgttcc attgcatgag
     8641 atgattcaat gccagcgcca aataaaccat gtctgatatc cgcaccagca tgtaaagctg
     8701 ctgaagcatc tgaaccataa tatggatata tgtctacttt atatggaata ttatttattt
     8761 tgcaaagatt aactaggtgt gatttcaatt gcttatgata tggacctgaa gcatctttgg
     8821 cacaaataga aactgtatat tcatccgatg cttggccgtc tcccaacgcg cccatatcta
     8881 atgcaatata ttctttgatt ttcgaatcaa ttgatgcatt tgcaccgtaa cctatttctt
     8941 cgttattaga aatataaaat tgcgttgtat gtggtaatat tatttgctct tcttttaatt
     9001 tctttagtaa ttttagtatc atcgctacgc tagctttatc atctaaatga cgagatttaa
     9061 taaaacctga tgacgtgata actgtacgtg gatcaaagct aacaaaatca cctactgaaa
     9121 tacctaaact ctttgtatct tcttctgatg tagttacttc atcaattctt atttccatat
     9181 gcttttggtc tctaggtatt tcatgattat ttctatatac atgaacactt gtttcatgca
     9241 gacaaatggt tcctgtatat atttgaccag catcagtttt aatttggcaa tattcaccct
     9301 caatcgcgtt atacgtgaat cctccaatta attctattgc taagcgccca tcttctttaa
     9361 tttctttaac cattgcacct aaagtatcaa catgagcagt aatacagcgt tgtacttcat
     9421 catttttgcc tggcactgtt attaataacg cacctttatt tgttagtgtt gtttgataac
     9481 ccaagtcttt tgcatatttt tcgacaaatt gaattgcttc ttctgtgtct cctgaaggac
     9541 tattaatacc tgttaatgtt ttaatcattt ctaatattgg ttccattgat ttacccctcc
     9601 gttgccattg tgttactata ctctttattg tagatgattt atatccgttt atctattaat
     9661 gctattatcg atttttattt actattgcac tgtgtcaaca gctcatttac aatgttatta
     9721 ttctgaaaat ttcgaaataa ggtgatttat atgacaacag ttttatttgt tgggcttgga
     9781 ttaattggtg gaagtcttgc tagcaatata aaataccata accctaatac taatattatt
     9841 gcatacgatg cagatacttc tcagttagat aaagctaaat caatcggtat tattaatgaa
     9901 aaatgtttaa attatagtga agctattaaa aaagccgatg taattattta tgcaacacct
     9961 gttgctatca caaataaata tcttagcgag cttatacata tgtcaactaa acctggtgtt
    10021 attgtttctg atactggtag tactaaagca atgatacagc aacacgaatg cagtttatta
    10081 aagcataata ttcatttagt cagtggtcat ccaatggctg gtagtcataa atccggtgta
    10141 ctaaatgcta aaaagcactt atttgaaaac gcttattata ttttagtcta caatgagcca
    10201 agaaatgagc aagcagcaaa cacgttaaaa gaactgttat cacctactct tgctaaattt
    10261 attgtaacta ctgctgaaga acacgactac gtaacaagcg tcgtaagtca tttacctcat
    10321 atcgttgcat ctagtttagt tcatgttagt caaaagaaca gtcaagaaca tcatttagtc
    10381 aataaacttg cagctggtgg ttttcgtgat atcactcgta tagctagtag taatgcacaa
    10441 atgtggaaag atatcacctt gagtaataaa acgtatattt tagaaatgat tcgacagcta
    10501 aaaagtcagt ttcaagattt agaaagacta attgaaagca atgattctga aaaattgtta
    10561 tctttttttg ccgaagctaa atcgtatcgt gacgcactgc ccgctaaaca actaggtgga
    10621 ctaaatactg cgtatgatct atatgtagat attccggatg aatcaggtat gataagcaaa
    10681 gtgacttata tcctaagttt acataatata tctataagca acttaagaat cttagaagta
    10741 cgcgaagata tatacggtgc tttaaaaatt agtttcaaaa atcctaccga tcgagaacgc
    10801 ggtatgcaag cattgagtga ttttgattgt tatatccaat aattattgag tctcttttga
    10861 taaattccat gtattcttag atattattgt gaaatctatt gtgatatatg aaatatttat
    10921 ctttagagac ttattttata cga
//
LOCUS       DAPIDM010000080        10931 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000080 DAPIDM010000000
VERSION     DAPIDM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.080"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..2186)
                     /locus_tag="RJE42_001779"
     CDS             complement(<1..2186)
                     /locus_tag="RJE42_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDV5877195.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATENKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQAAETQVE
                     VAQPRTVSESKPRVTRSADVVEAKEASDASEIKGTDVTSKVTVTESSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFEDGLKKGDYFDFTLSNNVNTYGVSTARKLPEIKNGSVVMATGQ
                     LLGDGKIRYTFTDYIDYKVNVIANLNLNLFIDPRIVKNNGEETLTSKLNGKNTEKKIE
                     VEYKDGVGKYYTNLNGSIETFNKADNKFTHVAYVKPINGNKSESVSITGSLTQGSNVS
                     GDSPIVKVYEYQGKETDLPKSVSVNLTDNSKFKDVTSDMQNKLTVQENGNYQLNLEKL
                     DKTYVIHYTGEYSKETDEVNFRTQVSAYPENSYRYYSYYNNHYTLTWDNGLVLYSNKA
                     DGNGQNGVKVESNNLIFDEVTGTGVITGQYDKNLVTTVEEETDSSTLDIDYHTAIDGE
                     GGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSK
                     HHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESN
                     LIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVI"
     gene            complement(2170..2272)
                     /locus_tag="RJE42_001780"
                     /pseudo
     CDS             complement(2170..2272)
                     /locus_tag="RJE42_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2655..4013)
                     /locus_tag="RJE42_001781"
     CDS             complement(2655..4013)
                     /locus_tag="RJE42_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDV5877196.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(4130..5683)
                     /gene="gntK"
                     /locus_tag="RJE42_001782"
     CDS             complement(4130..5683)
                     /gene="gntK"
                     /locus_tag="RJE42_001782"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV5877197.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDRYIIDYSMASATGMFNLETLDWDVGALELLGISEEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVAVYQELESIFINLSRSLTENYEQISDFQR
                     QHMAENKTQ"
     gene            complement(5708..6385)
                     /locus_tag="RJE42_001783"
     CDS             complement(5708..6385)
                     /locus_tag="RJE42_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5877198.1"
                     /translation="MYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMAK
                     QFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRVK
                     NQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHLK
                     PVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGKD
                     IEGFWLN"
     gene            complement(6542..7306)
                     /locus_tag="RJE42_001784"
     CDS             complement(6542..7306)
                     /locus_tag="RJE42_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDV5877199.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLD
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMISYGIGITIYYQRKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKTHMSTEQLEVPRTKNNN"
     gene            complement(7471..8163)
                     /locus_tag="RJE42_001785"
     CDS             complement(7471..8163)
                     /locus_tag="RJE42_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDV5877200.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(8394..8810)
                     /locus_tag="RJE42_001786"
     CDS             complement(8394..8810)
                     /locus_tag="RJE42_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDV5877201.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIVNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(8949..10772)
                     /locus_tag="RJE42_001787"
     CDS             complement(8949..10772)
                     /locus_tag="RJE42_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV5877202.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMILGTLTATTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGVLATMMVLITILLSKNKAEEQAIQNDFKLISKLLHAYN
                     DKSAFSQVAKTAVDSFKASDKLLITSNSSSDKLSGRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIITQRDDRNKNVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEAEMRKPLYSKRIYQNLTLDSIVFRNTLRYAAIMMIAIFIALMFDFEKAYW
                     IPLSAHTVLLGTSTIHAIERGIARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYTIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPYVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
BASE COUNT         3249 a         2148 c         1611 g         3923 t
ORIGIN      
        1 tcaatcacgc cataattacc gtgaccattt tcagttccta aaccagaatg agaaatatga
       61 tggttgtttt cagtgatttc ctcgattggt ccttgtgctt gaccatgttc ttcaggtaat
      121 tcatccacta attcgatcaa attactttca gttgtatatt ctttcgtatc ttcaattgtt
      181 gtatggtcgc tcactgcgcc agttacaata ccttttgtag actcttcgtc aaattcaact
      241 aagttagact cagttgttac ttggccacca cctgggtttg tatcctcttc atattcaaca
      301 acatcagcat gatgttttga attttcatgt gtagattctt caaagtcaat tggatttgat
      361 tcctcagagg actcagtgta tcctccaacg tgacccgctt cgctatccac agcagtatgg
      421 taatcgatat caatagctga tgaatccgtt tcttctattg tttcaatgta tccgtcaaca
      481 taaccacctt caccatctat agctgtgtga tagtcaatgt caagagttga tgaatctgtt
      541 tcttcttcaa cagtagttac taaattctta tcatattgac ctgtaataac tcctgttcct
      601 gttacctcat caaaaatcaa attattcgat tctactttaa caccattttg accattacca
      661 tctgctttat tactatataa aactaaacca ttatcccaag ttaatgtata gtgattatta
      721 taataagaat agtatctgta actattctcc ggatatgcag atacttgagt tctaaagtta
      781 acttcatctg tttcttttga gtactctcct gtgtaatgaa tcacatatgt cttatctaat
      841 ttttctagat tcaactgata atttccgttt tcttgtacag ttaatttatt ttgcatatca
      901 cttgtaacat ctttaaactt cgaattgtct gttaagttta cagaaacact ctttggtaaa
      961 tccgtttcct taccttgata ctcatacact ttaacaatgg gtgaatcacc acttacatta
     1021 ctaccttgtg tcaaactacc agtaatagat acactttcag atttatttcc gtttatcggt
     1081 ttaacgtaag ctacatgtgt gaatttatta tccgctttat taaatgtttc aattgaccca
     1141 ttcaggtttg tataatactt tccaactcca tctttatact caacttcaat ctttttttca
     1201 gtatttttcc catttaattt tgaagtaagt gtttcttcac cattattttt aacgattcta
     1261 gggtctataa ataaattcaa gtttaaattt gctattacat tcactttata atcaatataa
     1321 tctgtaaacg tgtatctaat ttttccatct ccaagaagtt gaccagtagc cattacgaca
     1381 gagccatttt taatctctgg taactttctc gctgttgaaa ccccataagt atttacatta
     1441 tttgataatg taaaatcaaa gtaatctcct ttttttagtc catcttcaaa tttcaattta
     1501 tattttagca ccgctctttg acctgcatga ggttctactt tatttgtatt attatgtcct
     1561 tcgatagagc tttcagtcac tgtaacttta cttgtcacat ctgtaccttt aatttctgaa
     1621 gcgtcactag cttccttagc ttcaactacg tctgctgatc ttgtcacacg tggtttactt
     1681 tctgataccg ttcttggctg cgccacttca acttgtgttt ctgctgcttg attttgtgta
     1741 gcctttttag gtgttaaatc tacttgtctt tgatctccgc tattgtcttg agattgtgtc
     1801 gtttccttaa cttgaggttt cgcttcttcc ttaactacct cttctttaac tgtttctaca
     1861 tttgctggtt gtgcagtttg tggtgcttgt actgcttttg gtgcttcttc agttgttact
     1921 tgtgttgcgt ttgacggttg ttctgttact gttgcgttat atgattgagt ttcttctata
     1981 tgattaacgt tagttgcagt tgtttgtgtt tcgcttgttt tattttcagt agctgaattc
     2041 ccattttctt ctactgtagt tgtcttttgt tctgatgctg cagcttcttt atcttgtccc
     2101 atcccaacaa cgatcattgt tcctaagaat actgatgctg ctcccaattt atgttttcta
     2161 atgccgtacc taagattgtt tttcactata atatctccct ttaaatgcaa aattcattaa
     2221 tttttttaaa cttaataaat gcaagtctat attgttcggt ttttaaaagc aatacaaaaa
     2281 agcgagttaa tgaaaagtta agattgtcat taattttatg ttaattgata attttattat
     2341 ttgaaatata cctataaatt gtattcaagt cgtcaaaaac ccttgtcaca caaggcttgt
     2401 attttttata cttattttta aaattaaatt catcattatc taatttaaaa caatatacta
     2461 agcgtttcat aattatcgcc tgtacaatac gcacaaaaac atgtcttgaa acgcctttca
     2521 ttacactaaa ataaccaata tactttttat atcgttcgga ttctaagtat ttcagacgat
     2581 tttctacata aaaataaacg tgtttcaagg caatatattg taattaccta aaaacacgtt
     2641 tacttaatat ttagttaaac aaataagctg atgaataaaa tgaagatgat acctgaaacg
     2701 gaaataatcg tttctaataa tgaccatgtt aggaatgttt cttttacagt taaaccaaaa
     2761 tattctttaa acatccagaa tcctgcgtca tttacatgag acaaaatcac actacctgca
     2821 cctatcgcaa gtacaactaa tgcaacattt acatctgatg attgtaataa tggtaagaca
     2881 atacctgtag ttgaaatcgc agctactgta gccgaaccta atgcgatacg tagcacagct
     2941 gcaacaatcc atgctagtaa aatcggagac atctctgtac cttcaaacat tttagcaatt
     3001 gtatttccga caccaccgtc aattaatact tgtttaaatg taccgccacc gccaataatc
     3061 aataacatca ttccgattgg ataaatcgca ttcgtcactg attccataat atgattcatc
     3121 ttacgttttc tcattaatcc catcgtaacg attgcaaata acactgctat tagcatggct
     3181 gtccctgctg ttccgatcat ataaatgata gattcaaata gatttgtagg tttgtcatgc
     3241 ccggttacaa gttgcgttat cgtagatact aacattaata tgactggtaa tgttgctgtt
     3301 aataaactca taccaaatcc tggcatctct tgatccgtaa attctttttg tgcacctaac
     3361 gctgaaatat cgccttctcg tgtatacgca gacggaatca ttttttgtgc aactttgtta
     3421 aatataggtc ctgcaatgag tgtaactgga atggcaataa tcataccgta tagtaataca
     3481 tctcctacat tcgcctttaa ttcttttgcg atgactaccg gtcctggatg tggtggtaaa
     3541 aagccatgtg tcactgataa agctgttacc ataggtagcc ctagttttaa cactgaaaca
     3601 tttgcgcgtt ttgctactgt aaatactaat ggaatcagta agactaaacc tacttcaaag
     3661 aacaatgcaa tcccgacgat aaatgctgca acaagcattg cccattgtac atgtttttga
     3721 ccaaattttt gaatcaacgt gtctgcaatt cgagttgcac caccaccatc agcaagtaat
     3781 ttcccaagta tggcacctaa accgaatatc agtgcaatgt gtccgagcgt actgcccatc
     3841 cctttctcaa tcgtctccat aattttagtc aatggtatac ctagcattaa cgctgtaatc
     3901 atcgatgtga taattaatga aataaatgta tttaatttaa acccaatgat taatactaat
     3961 aaaataacga tacctaaaac aacactgatt aacggccata tttcgttaaa catgacattc
     4021 ccctctttct cttttcaata gaatgtaaca ccgtcgtcgc gcacgtattc cacataatat
     4081 atagtagtca tggcacaaca actttatcta agatgcttga gtgacgtatt tattgtgttt
     4141 tattttcagc catatgttgg cgttgaaaat ctgaaatttg ttcataattc tctgttaaag
     4201 aacgacttaa attgataaaa atggattcga gctcttgata aacagcgaca ttttcttcaa
     4261 tcggcgtatg attgtttgtg gcaccgacca tcgatgaaac gattgaaaaa tcttcaatgt
     4321 cacctacagc tttaagcccg agtacgcagg cacctaagca tgaactttca taactttcag
     4381 gaaccactaa ctctgtgtca aatatatctg acatcatttg acgccatact tcacttttcg
     4441 caaaaccacc tgttgctttt atcatcttag gtgtttcatt cattacttca ataagcgcaa
     4501 gatagacggt atacaaattg taaagaacac cttctaatgc ggcgcgaatc atatgttctt
     4561 ttttatgaga taaagttaaa ccgaagaatg aacctcttgc atttgcgttc caaagcggtg
     4621 cacgttctcc tgctaaatag ggatgaaata ttaagccatc agcgccaggt ttaacacgct
     4681 ttgcaatttg agttaagaca tcataaggat caacaccgag acgtttcgca gtttcgactt
     4741 cacttgctaa caactcatcg cgcaaccatc tcaatacgac accaccatta tttacagggc
     4801 ctccaatgac gtagtggtcc tctgttaaga cataacaaaa tattctacct ttgtaatcag
     4861 tacgtggttt atctatcaca gtacgaatcg ccccagatgt accgattgtg acagcaactt
     4921 ctcctttacc aacactattg acacccaaat tagaaaggac cccatcactc gcaccaataa
     4981 caaacggtgt atcttcatta agccccatta atgttgcata acgttctttc atacctttca
     5041 tcacatacgt tgttggcact aattccggca acatttcctc ggaaatacca agcaattcta
     5101 atgccccaac atcccaatct aatgtttcta aattaaacat ccctgttgcg gaagccattg
     5161 aataatcaat gatatatcta tcaaacaaat gatagaaaat gtatgtttta atatctgcaa
     5221 acttagcagt acgttgaaat acatcttgcc attcatgttt catccaaaaa atcttcgcta
     5281 atggcgacat aggatgaata ggcgtacctg ttcgctgata aatcgcattg ccatcatgca
     5341 cttcatttat tactgttgca tattttgcag cgcggttatc tgcccaagta atattatttg
     5401 ttaatctttg atgttgctga tccatcgcaa tcaagctatg catttgcgca ctaaatgaca
     5461 caaatttaat atcatcttga ttaactttcg attccctcat tatgtattta attgtcatta
     5521 ataccgcatc aaataattca tctgggtttt cttctgagac atcaacgttt ggtgtgtgta
     5581 aatcatagcc gatttgatgt ttcatgatga aagttccatt ttcatcatat aagactgact
     5641 tggtactcgt cgttccaatg tcgacaccaa tcatatattt catgacaaat ccttctttct
     5701 tttcatttta attcaaccaa aatccttcaa tatctttacc aacatcgtcg aaatttaaat
     5761 gaaacgcttc tttcaaaatt tgactgtcgt attgttccac tgcatcaata aacacttgat
     5821 gattatgatg tatgcgttca aaatcttgcg ggttttgttg catacgttgt cgcatggatg
     5881 ttaaaacgag cgccatcatt actggtttta aatgactcca aaatgagttt aaatattgat
     5941 gattagatgc tttgattaat gtttcatgaa attcaaagtc atgcttcgta aatgattctg
     6001 catcctcaaa ttttactgcc actttcatca tttcaagttg tttcttcatt tcttttacga
     6061 taggtagtcg ctcttgattt ttaactcttg aaaatgcaaa tgactctagc atcagtcgca
     6121 aatcgtacat ttctttcttt tcttgttccc caaacggcaa cacatgtgca cccattcttt
     6181 ctaattggat gagttgattt tgttgcaata atttaaatgc atctcgaatt ggcgaacgac
     6241 tcacattaaa ttgctttgcc atttgatttt cagtgagtaa cgtaccttca gctatgtgac
     6301 cattcacaat gcctaagcgt aattctgctg caataccttc tccagttgtc ataccttcca
     6361 accatttctc tggatatcca tacatcgtca aagtcactcc ttcattacac gacatacttg
     6421 tatacaagta tgttaatata gttctcaagt gtttgcaagc gctttcttta cgcgtaataa
     6481 aatagtgacc acccctcttc gatgtatatt taaagggaat ggtcactatg acacgaatga
     6541 tttaattgtt atttttagtg cggggtactt ctaattgttc tgtactcata tgtgttttag
     6601 gtacttcaat gcaataatgc gtttcatgac agtttggaca ttcgaatcga cgtgttgtcg
     6661 ctgtatgttt cgctttgata actgcccaca aagacggtga gaatatatgc tggcagttag
     6721 ggcataaata ggcaactttg cgttgatagt agatagtaat accaatgcca taactaatca
     6781 taaatggtaa agcaattaaa aacggccatt tatttttcat caaaattgca cttataatgc
     6841 tagaatattg aattattcct ataataccag cactaatcca aatattacga cgaatacttt
     6901 tcatttcagc tgatttactc atgacatgct ctatgtcttt taaatgtgtg attggagacg
     6961 tcgacgcttc atttacgtaa tattgaacat ttttaatttt gtctaatacc gcttgttgct
     7021 gtttgacttg ttgattaatt tcttgttgtt tcatagttag taaggtattg agtgtcttca
     7081 aagtaccttc accttttagc aacatatcta tatcgcttaa cgcacaacct aaatctttaa
     7141 gcaataagat taactctaat gtttgtcgct gttgttctgt atacacacga cgctttcctt
     7201 ctgtaaatcc ttgtggtttc aaaatacctt tgcgatcata atattgaatc gttcgtgttg
     7261 tcacattgca taattttgcg agttctccag tcgaatagtt agacatagat tccacctcct
     7321 acaatcacca tagttgatga cccgacgtca cgagcaagta taatttccac atttttaaga
     7381 aatttattat actaggcgtc ttatttttgt gatttcatac catgttgatt tacaaaatca
     7441 ctcaaattaa gcaacacacc tactaaacgt ctactctgtt atttcagaat gaatttgttg
     7501 taatttatct tcaacttcag taatctctgt cgcacattct ttcaataaat ctcgatactt
     7561 ttctgtctct gcattgtttt tataacgtat tttatgttct aaacttgccc acatatccat
     7621 acctattgtt ctaatttgaa tttcaacagg caatacctca acgcgttctg ctaaaaagac
     7681 tggaatggat acaacgatat gtaaactgcg gtaaccattt tctttagggt gctgaatata
     7741 atctttacgt tttatcaatt gtacgtcttc ttgtttaagc aacatctctt caatcaaata
     7801 aatatcatct aagtaattac atactacgcg aattccggca atatccagta tatgttcttt
     7861 ggcagtttct gcgctaatct gtaatccttt acgattaagc ttttctatta aactacgcat
     7921 ttctttcaca cgtcgctcca tatgatgtat agggttgtgt ttataaatgt gattgaaatt
     7981 gtcgtctaaa atactcagtt tagtgcttat ttcctttagt gctgatgaat aaatatgatc
     8041 taactctacg aaacctaata acgtatcaaa tgcttcatca ccattttcaa atttacttaa
     8101 actattttta aaatcatgtc gcaaatcctc taaatatagt gatggttttc gatctacata
     8161 catttttata cttacctccg atatataatc atatttattg tacctaacat tttattaaga
     8221 taatactaat acactatcga actttgggct gatactgaaa cagcgcaaag aaactcaaac
     8281 aattcgaatt atgtatcaaa accttcaatt atgggaagta attttaaaat gatttaacgc
     8341 aaaaaaacac ctgttaccgt tatataggta caagtgctta attgatagag atgttatacg
     8401 tctttaaatg attccacaac ttttggatgc ggaccatcca taagcggttc tctttgtcga
     8461 acgccaccac tttggttgac aattgattcg ctatcaaagt acgatttatc ttgatttgat
     8521 tgttcttgaa tatgttcttc attatcagtt gttgcatatt cactatccac ctctgttttt
     8581 tccattgttg ctgtatggaa ttgtacgaag ttctcttctt ctaatgcttt gatttcttct
     8641 ttcgataacg ctcgatacca atctttcgct tttttcgccg caataggaac aacatctttt
     8701 tcaagatatc cttcttttaa cattgcattc gcaatttcta tcgctttttg ccgtttaatt
     8761 acttcaaatt ctttccattt ttcggggtaa cctttcattg taaagggcat ttccttaacc
     8821 tccaatatgt tataattcat gttatatacc accataatta taaaatgaaa catattaaac
     8881 acaatttttt agactcccgt cataataact tcagacaaac gttagtctga cctgataaat
     8941 attttatttt aaacgttctt gtctctctca cgaaaaccat tgtgttgaat attcatcatt
     9001 gctgattgaa tataattata ctgtggtaaa tctggtaata tctttatttc taattctgta
     9061 tttaattcta aatgtttagc gatgttttca aatgttgcta aatactcgcc cattaattgt
     9121 tcatttattg taagtctatc ttcattagcc atggctctat ttaacataaa gctaatttct
     9181 tctaatgcga ataagctagg ataataattt tgaatcaatg tcttatcact aaataattca
     9241 ccattagctg cattataaac ttgcgtcatg ttatttaatt tcacagatag gttcatagct
     9301 gtgtttttct gatacacatc atctttatat tggttttcag aaaataaata atgaaagagt
     9361 gttgcttctt ttcgaacaac ttcagccatt acataaggaa gcaatgcgga tgcggtacgt
     9421 tgcccaagga caaataaacc tatgattgca atcacaatcc ccattgcaac gtcaataact
     9481 cttggaaacg caatgttaat tgttaagttc tgtgatgcta atccgttcat taaaataact
     9541 tgaattgtaa taaaaactac tgcaattgta taatttgctc ccaccaacgc ttcagtaaat
     9601 aatgctgcaa tacccattaa aatgactgct acaggtgttg gtattgaaaa caacaatata
     9661 actgaaagta ctagcacacc taaaatggtg cctaatccac gtgcaatacc tctctcaatt
     9721 gcatgtatag ttgatgttcc taataatact gtatgtgcgg ataaaggtat ccaatatgct
     9781 ttttcaaaat cgaacattaa cgcgataaat atcgctatca tcataatcgc tgcatacctc
     9841 aatgtatttc taaacacgat tgaatctaat gttaaatttt gataaatacg cttactatat
     9901 aaaggttttc gcatttcagc ttcatacgca atacgcgctt catttgcatg caccatttcg
     9961 tcaattttca agatatggtt gaataagttt tgaaattctt ctgttactgt tacttctttg
    10021 cgccaatatc tcacattttt attgcgatca tctcgctgtg taatgatatg atccatcatt
    10081 tcaattaact catctggcaa tggtcgaatt tgtttagcat tcaactctaa cagttcagaa
    10141 taaatccctt gggcagatgt gtgtaataat aataattttt ggaaacgtcc gcttaattta
    10201 tcactacttg aattagaagt gatgagtagt ttatccgacg ctttaaaact atccactgca
    10261 gtctttgcta cttgtgaaaa tgcagattta tcattgtaag catgcaataa cttagatatg
    10321 agtttaaaat cgttttgaat ggcttgttct tcagctttgt ttttagacaa taagattgtg
    10381 atgagtacca tcatagtggc aagtacacca cctactaaaa tcgcaaagcc tctatataat
    10441 gcttcttcag gtgctatagg taagttaatg ggtaaactga atgtcacaat gaagaatgtc
    10501 gatgacggtc cagcaatttt caaggcggta aatatataaa atggtataac cgtaacaatt
    10561 aataatgtca ttccaaaaac gagcggcgtt gtggctgtta gcgtaccaag tatcatgcat
    10621 attgcaaacg ctaaattaca aattattaca gttcgcagct tagatcgcga cggaccttta
    10681 aaaacgtaga tatgtgctag agttccggtt gcaactaata atccaaattg gaaattacca
    10741 cataagtaac cgatgattgc aggaataatc attaacagtc cttgtcttat ccctttataa
    10801 aagtctaagt taagtcgatt aatttttata agtgatgtca aaaagatgtc atttcgactt
    10861 ctcccttcct cgtcaaaata tttccataat cattttatta gcatagttaa tttgagtatg
    10921 ttgctataaa t
//
LOCUS       DAPIDM010000081        10902 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000081 DAPIDM010000000
VERSION     DAPIDM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.081"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            219..1907
                     /gene="ilvD"
                     /locus_tag="RJE42_001788"
     CDS             219..1907
                     /gene="ilvD"
                     /locus_tag="RJE42_001788"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV5877203.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAISDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1934..3703
                     /gene="ilvB"
                     /locus_tag="RJE42_001789"
     CDS             1934..3703
                     /gene="ilvB"
                     /locus_tag="RJE42_001789"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDV5877204.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYGVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDTAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            3703..3957
                     /locus_tag="RJE42_001790"
     CDS             3703..3957
                     /locus_tag="RJE42_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDV5877205.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4079..5098
                     /gene="ilvC"
                     /locus_tag="RJE42_001791"
     CDS             4079..5098
                     /gene="ilvC"
                     /locus_tag="RJE42_001791"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV5877206.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNTLAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            5128..6657
                     /locus_tag="RJE42_001792"
     CDS             5128..6657
                     /locus_tag="RJE42_001792"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV5877207.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVIKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6660..7706
                     /gene="leuB"
                     /locus_tag="RJE42_001793"
     CDS             6660..7706
                     /gene="leuB"
                     /locus_tag="RJE42_001793"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV5877208.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7720..9090
                     /gene="leuC"
                     /locus_tag="RJE42_001794"
     CDS             7720..9090
                     /gene="leuC"
                     /locus_tag="RJE42_001794"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV5877209.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEISDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9091..9663
                     /gene="leuD"
                     /locus_tag="RJE42_001795"
     CDS             9091..9663
                     /gene="leuD"
                     /locus_tag="RJE42_001795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV5877210.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPKYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9678..>10902
                     /gene="ilvA"
                     /locus_tag="RJE42_001796"
     CDS             9678..>10902
                     /gene="ilvA"
                     /locus_tag="RJE42_001796"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDV5877211.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNQFDPSN"
BASE COUNT         3704 a         1794 c         2110 g         3294 t
ORIGIN      
        1 catcaaccga caacttcaaa tattattatt ttattatact ctttaggact cgaacgttag
       61 taaatattta ctaaacgctt taagtcctat ttctgtttga atgggacttg taaacgtccc
      121 aataatattg ggacgttttt ttatgtttta tctttcaatt acttattttt attactataa
      181 aacatgatta atcattaaaa tttacggggg aatttactat gcgaagcgac atgatcaaaa
      241 aaggagatca ccaagcacca gcaagaagtc ttttacatgc cacgggcgcg cttaaaagtc
      301 caactgatat gaacaagcca tttgtagcta tttgtaactc ttatattgat atcgttccag
      361 gacatgttca tttaagagag cttgcagata tagctaaaga agcaattaga gaagccggtg
      421 ccattccatt tgaattcaat acaattggtg ttgatgatgg aatagctatg ggacatatag
      481 gaatgcgata ttctctacca tcacgtgaaa ttattgcaga tgcagctgaa actgtaatta
      541 acgctcattg gtttgacggc gtattttaca ttcctaattg tgacaagatt acacccggta
      601 tgattttagc agccatgagg acaaacgtac cagctatctt ttgctctggt ggaccaatga
      661 aagctggctt atctgcacat ggtaaagcat taacactttc atcaatgttt gaagctgtag
      721 gcgcttttaa agaaggatcg atttctaaag aagaattttt agatatggaa caaaatgcct
      781 gccctacttg tggttcatgt gctgggatgt ttactgcaaa ttcaatgaac tgtttgatgg
      841 aagttttagg tctagcatta ccatacaacg gtactgcact tgcaatcagt gatcagcgac
      901 gcgaaatgat tcgccaagca gcttttaaat tagttgaaaa tattaaaaat gatttaaaac
      961 cacgtgatat cgttactcgc gaagcaattg atgatgcatt tgcacttgat atggctatgg
     1021 gtggttcaac aaacacagta ctgcatacgt tagccattgc caatgaagct ggtattgatt
     1081 atgacttaga gcgcattaat gctattgcca aacgcacgcc atatttatca aaaatagcac
     1141 ctagttcatc gtattcaatg catgatgtgc atgaagctgg tggcgtccca gcaattatta
     1201 atgaattgat gaagaaagat ggcacgttac acccagatag aatcacagtt actggcaaaa
     1261 cgttacgtga aaataacgaa ggcaaagaaa ttaagaactt tgatgtcatt caccctcttg
     1321 atgcaccata tgatgcacaa ggtggtttat ctatcttatt tggtaatatc gcaccaaaag
     1381 gcgcagttat taaagttggc ggcgttgatc catctatcaa aacatttact gggaaagcaa
     1441 tttgtttcaa ttcgcatgat gaagctgttg aagcaataga caatcgtacc gttcgtgcag
     1501 gccacgtcgt tgtcattaga tatgaaggac caaaaggtgg accaggtatg cctgaaatgt
     1561 tagcacctac ttcctctatt gttggtcgtg gcttaggtaa agatgttgca ttaattactg
     1621 atggtcgttt ttccggtgcc acaagaggta ttgcagttgg tcatatttcc cctgaagctg
     1681 catctggtgg accaattgcc ttaattgaag atggagatga gattactatt gatttaacaa
     1741 atcgtacatt aaacgtaaac cagcctgaag atgttctagc gcgtcgccga gaatctttaa
     1801 caccatttaa agcgaaagta aaaacaggtt atctagctcg ttatactgcc ctagtaacta
     1861 gcgcaaatac aggtggcgtc atgcaagtcc ctgagaattt aatttaattt attttatatt
     1921 ggagatggtt aaaatgtcta aaactcaaca tgaagtaaac caaaatattg accctttaaa
     1981 aatggctgaa tcacttgaac ctgaacaact aaatgaaaaa actttaaatg acatgcgttc
     2041 aggatcagaa gttctagtag aagctctact taaagaaaat gtggattatt tattcggtta
     2101 tcctggtggt gccgtactac ctttatacga cacattttat gatggtaaaa tcaaacatat
     2161 tttagcaaga cacgaacaag gtgctgttca tgctgcagaa ggttatgcac gtgtatctgg
     2221 taaaactggc gtcgttgtag ttacaagcgg tccaggtgca acaaatgtaa tgacaggtat
     2281 tacggatgca cattgcgact ctttacctct agttgtattc actggacaag ttgctacacc
     2341 aggcattggt aaagatgcat tccaagaagc ggatattcta tctatgacat cgccaattac
     2401 aaaacaaaat tatcaagtga aacgtgttga agatatacct aaaatcgtac acgaagcttt
     2461 ccatgtagct aattctggac gcaaaggtcc tgtagtgatt gattttccaa aagatatggg
     2521 tgttttagct acaaatgtag atttatgcga cgaaatcaat attccaggtt atggagttgt
     2581 tacagaacca gaaaataaag acattgacac tttcatctca cttttaaaag aagcgaaaaa
     2641 gcctgtcgta ttagccggcg caggtattaa tcaatcaaaa tcaaatcaat tattaacaca
     2701 gtttgttaat aaacatcaaa tcccaacagt tactacttta ctaggtctag gtgctgtacc
     2761 ttatgaggat acactatttt taggtatggg aggtatgcat ggttcttatg ctagtaacat
     2821 ggcattaact gagtgtgatt tactcattaa tttaggtagc cgcttcgatg atagattagc
     2881 aagcaaacct gatgcctttg cacctaacgc caaaattgta catgtagata ttgatccttc
     2941 agaaatcaat aaagttattc atgtagattt aggaattatt gcagattgta aaagattttt
     3001 agaatgttta aatgataaaa atgttgagac tatagaacac agtgactggg ttaaacattg
     3061 tcaaaataat aagcagaaac acccatttaa acttggtgaa gaagatcaag tattttgtaa
     3121 gccacaacaa acaatcgaat atattggcaa aattacaaat ggtgaagcaa ttgttactac
     3181 agacgtggga caacatcaaa tgtgggcagc tcaattttat ccatttaaaa atcacggaca
     3241 atgggttaca agcggtggtt taggaacaat gggattcggt attccttcgt caattggtgc
     3301 caaattagct aatcctgata aaacagtcgt atgtttcgtt ggtgacggtg gtttccaaat
     3361 gacaaaccaa gaaatggcac tattacccga atatggttta gatgtcaaaa tcgtactaat
     3421 caataatgga acattaggta tggttaaaca atggcaagat aagttcttta atcaacgctt
     3481 ctcacactca gtatttaatg gtcaacctga ttttatgaaa atggcagaag catatggcgt
     3541 caaaggtttc ttaatcgata agccagaaca actggaagaa caattagata cagcgtttgc
     3601 ttatcaaggg ccagctttaa ttgaggttcg tatttcccct actgaagctg taaacccaat
     3661 ggttccgagt ggcaaatcaa atcatgaaat ggagggctta taatgacaag aattcttaaa
     3721 ttacaagttg cggatcaagt cagcacgcta aatcgaatta caagtgcttt tgttcgccta
     3781 caatataata tcgatacatt acatgttaca cattctgaac aacctgggat ttctaacatg
     3841 gaaattcaag tcgatattca agatgataca tcacttcata ttttaattaa aaaattaaaa
     3901 caacaaatta atgttttaac ggttgaatgc tacgaccttg ttgataacga agcttaattt
     3961 taagacaaag gcaatgatgc gctaattagt tatagatata tcataggctg ctagttaaca
     4021 tctgccacta ttacaaagtt atatttcaga attttcgaaa aacaaaacat ttaattattt
     4081 ggaggaattt attatgacaa cagtttatta tgatcaagat gtaaaaacgg acgctttaca
     4141 aggcaaaaaa attgcagtag taggttatgg atcacaaggc cacgcgcatg cacaaaactt
     4201 aaaagacaat ggatatgatg ttgtcatcgg tattcgacca ggtcgttctt ttgacaaagc
     4261 taaagaagat ggatttgatg tgttccctgt tgcagaagca gttaagcaag ctgatgtaat
     4321 tatggtgcta ttacctgatg aaattcaagg tgatgtttac aaaaacgaaa ttgaaccaaa
     4381 tttagaaaaa cataatacgc ttgcatttgc tcatggcttt aacattcatt ttggtgttat
     4441 tcaaccacca gctgatgttg atgtattttt agtagctcct aaaggaccgg gtcatttagt
     4501 tagacgtaca tttgttgaag gttctgctgt accatcacta tttggtattc aacaagacgc
     4561 ttcaggtcaa gcacgtaata ttgctttaag ttatgcaaaa ggtattggtg caactcgtgc
     4621 aggtgttatt gaaacaacat ttaaagaaga aacagagaca gatttatttg gtgaacaagc
     4681 agtactttgc ggtggtgtat cgaaattaat tcaaagtggc tttgaaacat tagtagaagc
     4741 gggttatcaa ccagaattag cttattttga agtattacat gaaatgaaat taatcgttga
     4801 tttgatgtat gaaggcggta tggaaaatgt acgttactca atttcaaata ctgctgaatt
     4861 tggtgactat gtttcaggac cacgtgttat cacaccagat gttaaagaaa atatgaaagc
     4921 tgtattaact gatatccaaa atggtaactt cagtaatcgc tttatcgaag acaataaaaa
     4981 tggattcaaa gaattttata aattacgcga agaacaacat ggtcatcaaa ttgaaaaagt
     5041 tggtcgtgaa ttacgcgaaa tgatgccttt tataaaatct aaaagcattg aaaaataaga
     5101 tagacctaca atgaggagtt gttaaatatg agtagtcata ttcaaatttt tgatacgaca
     5161 ctaagagacg gtgaacaaac accaggagtg aattttactt ttgatgaacg cttgcgtatt
     5221 gcattgcaat tagaaaaatg gggtgtagat gttattgaag ctggatttcc tgcttcaagt
     5281 acaggtagct ttaaatctgt tcaagcaatt gcacaaacat taacaacaac ggctgtatgt
     5341 ggtttagcta gatgtaaaaa atctgacatc gatgctgtat atgaagcaac aaaagatgca
     5401 gcgaagccgg tcgtgcatgt ttttatagcg acatcaccta ttcatcttga acataaactt
     5461 aaaatgtctc aagaagacgt tttagcatct attaaagaac atgtcacata cgcgaaacaa
     5521 ttatttgacg ttgttcaatt ttcacctgaa gatgcaacgc gtactgaatt accattctta
     5581 gtgaaatgtg tacaaactgc cgttgacgct ggagctacag ttattaatat tcctgataca
     5641 gtcggctaca gttaccatga tgaatatgca catattttca aaaccttaac agaatctgta
     5701 acatcttcaa atgaaattat ttatagtgct cattgccatg acgatctagg aatggctgtt
     5761 tcaaatagtt tagctgcaat tgaaggcggt gcgagacgaa ttgaaggcac tgtaaatggt
     5821 attggtgaac gagcaggtaa tgcagcactt gaagaagtcg cgcttgcact atacgttcga
     5881 aatgatcatt atggtgctca aactgccctt aatctcgaag aaactaaaaa aacatcggat
     5941 ttaatttcaa gatatgcagg tattcgagtg cctagaaata aagcaattgt tggccaaaat
     6001 gcatttagtc atgaatcagg tattcaccaa gatggcgtat taaaacatcg tgaaacatat
     6061 gaaattatga cacctcaact tgttggtgta agcacgactg aacttccatt aggaaaatta
     6121 tctggtaaac acgccttctc agagaagtta aaagcattag gttatgacat tgataaagaa
     6181 gcgcaaatag atttatttaa acaattcaag gccattgcgg acaaaaagaa atctgtttca
     6241 gatagagata ttcatgcgat tattcaaggt tctgagcatg agcatcaagc actttataaa
     6301 ttggaaacac tgcaactaca atatgtctct agcggccttc aaagtgctgt tgttgttatt
     6361 aaagataaag agggtcatat ttaccaggat tcaagtattg gtactggttc aatcgtagca
     6421 atttacaatg cagttgatcg tattttccaa aaagaaacag aattaattga ttatcgtatt
     6481 aattctgtca ctgaaggtac tgatgcccaa gcagaagtac atgtaaattt attgattgaa
     6541 ggtaagactg tcaatggctt tggtattgat catgatattt tacaagcctc ttgtaaagca
     6601 tacgtagaag cacatgctaa atttgcagct gaaaatgttg aaaaggtagg taattaatta
     6661 tgacttataa cattgttgcc ctacctggtg atggaatcgg tccagaaatt ttgaacggat
     6721 ctctatcctt gcttgaaatt ataagtaata aatataactt taattatcaa atagagcacc
     6781 acgaatttgg tggtgcctct attgatacat tcggcgagcc tttaactgag aaaaccttaa
     6841 atgcgtgtaa aagagcagat gctattttac tgggtgcaat cggtggacct aaatggacag
     6901 atcctaacaa tcgaccagaa caaggattat taaaattacg taaatcatta aatttatttg
     6961 caaatatacg ccccactacc gttgtcaaag gcgctagttc tttatcacct ttaaaggaag
     7021 aacgcgttga aggcacagat ttagttatag tccgtgaatt gacaagtggt atttattttg
     7081 gagaacctag acattttaat aatcacgagg ccttagattc tcttacttat acaagagaag
     7141 aaatagaacg cattgttcac gtagcattta aattggccgc ttcaagacga ggaaaactaa
     7201 catcagttga taaagaaaat gtattagctt ctagtaaatt gtggcgcaaa gtcgtaaatg
     7261 aagtgagtca attatatcca gaagtaacag taaatcactt acttgttgat gcttgtagta
     7321 tgcatttaat cacaaatcca aaacaatttg acgtcatcgt ttgtgaaaac ttatttggcg
     7381 atattttaag tgatgaagct tcagtgattc ctggttcact tggtttatca ccttctgcta
     7441 gttttagtaa cgatggtcca agattatatg agcctattca tggatcagca ccagatattg
     7501 caggtaaaaa cgttgccaat ccatttggaa tgattctatc tttagcgatg tgtttacgtg
     7561 aaagcttaaa tcaaccagat gctgcagatg aattagaaca acatatttac aacatgattg
     7621 aacatgggca aacgacagca gatttaggcg gcaaactgaa tactactgat attttcgaaa
     7681 ttctatctca aaaattgaat cactaagggg gagatgtaaa tgggtcaaac attatttgac
     7741 aaggtgtgga acagacatgt gttatacggg aaattgggcg aaccgcaact attatacatt
     7801 gatttacacc ttatacatga agttacttct cctcaagcat ttgaaggact taggcttcaa
     7861 aacagaaaat taagacgccc agatttaaca tttgcaacac tcgatcacaa tgttcctact
     7921 attgatattt tcaatattaa agatgaaatt gcaaacaaac aaatcacaac attacaaaaa
     7981 aacgccatag attttggggt gcatattttt gatatgggtt ctgatgaaca aggtattgtt
     8041 cacatggtag gacctgagac aggacttaca cagcctggca aaacaatcgt ttgtggtgac
     8101 tctcacacag caacacatgg agcatttggt gctattgcat ttggaattgg aacaagtgaa
     8161 gttgaacatg ttttcgcaac tcaaacgcta tggcaaacaa aacccaaaaa cttaaaaatc
     8221 gatattaatg gtaccttacc aacaggcgtc tatgctaagg acattattct gcatttaatt
     8281 aaaacgtatg gtgttgactt tggtacaggc tatgctttgg aatttactgg cgaaacaatt
     8341 aaaaaccttt caatggacgg tcgaatgact atttgtaaca tggctatcga aggtggtgcc
     8401 aaatacggca taatccaacc tgacgatata acatttgaat atgttaaagg gagaccattt
     8461 gctgataact tcgctaaatc agttgataag tggcgtgagc tatattctga tgacgacgcg
     8521 atatttgatc gtgtaattga acttgatgtt tcaacattag aaccacaagt gacatgggga
     8581 actaatcctg aaatgggtgt taatttcagt gaaccattcc ctgaaatcag tgatatcaac
     8641 gatcaacgtg cgtatgatta tatggggtta gagccaggac aaaaagctga agatatcgac
     8701 ttagggtatg ttttcctcgg ttcatgtaca aatgctagac tttcagattt gattgaagct
     8761 agtcatattg ttaaaggaaa taaagttcac ccaaatatta cagctattgt cgtaccaggt
     8821 tctcgtacag taaaaaaaga agctgaaaaa ttaggtctag atactatctt taaaaatgca
     8881 ggatttgaat ggcgtgaacc aggatgttca atgtgtttag gcatgaatcc tgaccaagta
     8941 cctgagggcg tacattgtgc atctacaagt aatcgaaact ttgaaggacg acaaggcaaa
     9001 ggtgctagaa cacatttagt atcccctgct atggcagcag cagcagctat tcatggtaaa
     9061 tttgtggacg taagaaaggt ggttgtttaa atggcagcaa tcaaacctat tacaacatat
     9121 aaaggtaaaa tagtccctct tttcaacgat aatatcgata cagatcaaat cattcctaag
     9181 gtacacttaa agcgtatttc aaaaagtggc tttggtccat ttgcttttga tgaatggcgg
     9241 tatttacctg atggttcaga taatcctgat ttcaatccaa ataagcctaa atataaaggt
     9301 gcatccatat taattactgg agataatttt ggatgtggtt caagtcgtga acatgcagct
     9361 tgggcactta aggactatgg ctttcatatt attattgcag gaagtttcag tgacattttt
     9421 tatatgaatt gtacaaaaaa tgcaatgtta ccaattgttt tagaaaaaaa tgcacgtgaa
     9481 catcttgcta aatatgttga aattgaggtc gatttaccaa atcaaactgt gtcatcacca
     9541 gacaagagtt tccattttga aattgatgaa acttggaaga ataaacttgt aaatggctta
     9601 gatgacattg caatcaccct acaatatgaa tcattaatag aaaaatatga aaaatcactt
     9661 taagggagtt gaatattatg acagtcaaaa caacagtttc tacgaaagat atcgatgaag
     9721 catttttaag acttaaagat attgtcaaag aaacaccttt acaattagac cattacttat
     9781 ctcaaaagta tgattgtaaa gtttatttaa aacgagaaga tttacaatgg gtacgttctt
     9841 ttaaattaag aggtgcttac aacgctattt ctgttttatc agatgaagct aaaagtaaag
     9901 gtattacatg tgcgagtgca ggtaatcatg ctcaaggtgt tgcctataca gctaaaaaac
     9961 ttaatttaaa cgctgttatc tttatgccag tcactacacc tttacaaaag gtaaatcaag
    10021 taaagttctt tggaaatagt aacgttgaag ttgtactcac tggtgataca tttgatcact
    10081 gtttagctga agctttaact tatacaagtg aacatcaaat gaactttata gatccattca
    10141 ataatgttca tacaatttct ggacaaggta cgcttgctaa agaaatgcta gaacaagcaa
    10201 agtctgacaa tgttaacttt gattatctat ttgccgcaat tggtggtggt ggtttaattt
    10261 caggtattag tacctatttt aaatcctatt ctcctaacac aaaaattatc ggagttgaac
    10321 cttcaggtgc tagtagtatg tatgaatctg ttgtcgtaaa taatcaagtt atcacattac
    10381 ctaatatcga taaatttgtc gatggagctt ctgtagccag agttggcgat attacttttg
    10441 aaattgccaa aaaaaatgta gataactacg tacaagtaga tgaaggtgct gtatgttcaa
    10501 caattttgga tatgtattca aaacaagcaa tagtagcaga acccgctggc gctttaagtg
    10561 taagtgcgct tgaaaactat aaagatcata ttaaaggtaa aacagtggtt tgtgtcatta
    10621 gtggtggtaa taatgatatt aatcgaatga aagaaattga agaacgttca ttactatatg
    10681 aagaaatgaa gcattacttt atcttaaatt tccctcaacg tccaggtgca ttgagagaat
    10741 ttgtaaatga cgtattagga cctcaagacg atattactaa atttgaatac ttaaaaaaat
    10801 cttctcaaaa tacaggtact gtcattattg gtattcaact taaagatcat gatgatttaa
    10861 tacaactcaa acaacgtgta aatcaattcg atccttccaa ta
//
LOCUS       DAPIDM010000082        10874 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000082 DAPIDM010000000
VERSION     DAPIDM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.082"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(79..462)
                     /locus_tag="RJE42_001797"
     CDS             complement(79..462)
                     /locus_tag="RJE42_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV5877212.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQAYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDIQYLLQSRVLNTKRKR"
     gene            645..2087
                     /locus_tag="RJE42_001798"
     CDS             645..2087
                     /locus_tag="RJE42_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDV5877213.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSEMEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            complement(2173..3117)
                     /locus_tag="RJE42_001799"
     CDS             complement(2173..3117)
                     /locus_tag="RJE42_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDV5877214.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3163..3786)
                     /locus_tag="RJE42_001800"
     CDS             complement(3163..3786)
                     /locus_tag="RJE42_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5877215.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            3947..5224
                     /locus_tag="RJE42_001801"
     CDS             3947..5224
                     /locus_tag="RJE42_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDV5877216.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTNQNVKVNAADINGLTNPVKVD
                     NKKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5430..5861
                     /locus_tag="RJE42_001802"
     CDS             5430..5861
                     /locus_tag="RJE42_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDV5877217.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            5966..6082
                     /locus_tag="RJE42_001803"
     CDS             5966..6082
                     /locus_tag="RJE42_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877218.1"
                     /translation="MANYAIYFNNININSIMSLNDNNYQFERVNERNEYRES"
     gene            6093..7127
                     /locus_tag="RJE42_001804"
     CDS             6093..7127
                     /locus_tag="RJE42_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5877219.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKSERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            7163..7564
                     /locus_tag="RJE42_001805"
     CDS             7163..7564
                     /locus_tag="RJE42_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877220.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDVAVLKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYAKFGFIPTEPDSGGMYMKF"
     gene            7729..7905
                     /locus_tag="RJE42_001806"
     CDS             7729..7905
                     /locus_tag="RJE42_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877221.1"
                     /translation="MKIDTLRQAIIYYFVALVLSALMIYLFLTNLERVILYSCVFTVV
                     YWVIYAFDVYRKRN"
     gene            complement(8130..9134)
                     /locus_tag="RJE42_001807"
     CDS             complement(8130..9134)
                     /locus_tag="RJE42_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV5877222.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIVKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSNQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            9325..9840
                     /locus_tag="RJE42_001808"
     CDS             9325..9840
                     /locus_tag="RJE42_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877223.1"
                     /translation="MAGIIKEISKQDASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVGKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQCIYFLKDF
                     QGGGRGSQLIALAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            10156..10785
                     /locus_tag="RJE42_001809"
     CDS             10156..10785
                     /locus_tag="RJE42_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877224.1"
                     /translation="MKKLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDNSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSNSGQTTN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKSQALQQLPNFQTALNAANNEANKFGNGHK
                     VYNDYSIEEHNGNYKYVFSFKDPNVNGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         3748 a         1525 c         1951 g         3650 t
ORIGIN      
        1 tatagtaaac tttagtacaa agtatgaatt gctgaactcc aatgactact atttgtccgt
       61 tacaactaaa gtttcaatct atctctttct ttttgtgttt aaaacacgac tttgtagtaa
      121 atattgaata tcatatttat tagcaagttc tgctaaaaaa tctaataaag tttgggtgtc
      181 gattttacca ttaataaaat cagctttaag tagttttgat tgttgtcttt cttcttcatt
      241 cgcacatttt ttaaaatatc cccacatatg atcaaaagca ttacatacac ttcctatact
      301 cggttcaatt aacaaagcat catcaatcat ttgttggact tgatgaagtg ataaatccgt
      361 ttttaaagca tttcttatca tttggtaagc ctgttgactg tgtaacaata catgatattt
      421 ttcctcacgc caaagttgtt cgatatggcc acgttctttc ataaaattac ctccgataat
      481 taatgttaat atacatgcat gtagtatagc aataaaaatg aatgcgctat caaaagtgct
      541 gtaattgttt atataaaatg ataatagagt gttgcaaata tttttggaac cggttacaat
      601 aaagtggaat aaatggaacc ggttctaaat aggaggctaa atatatgaat tataagcaat
      661 ccgcagaaga tattttgaac gcgataggcg gagaagagaa tttagatgca atggcgcatt
      721 gtgcaacgag actacgatta gttttaaatg atgaaagttt agtaaatgaa gaggcgctaa
      781 acaatatgga tgtagttaaa gggacatttt ctactggggg acaataccaa attattattg
      841 ggtctggtac agtcaataaa gtatttagtg aaatggaaaa attaactgga aaagaagcat
      901 caaccacttc ggaagtcaaa gcacaatctg ctaaaaatat gaatccgtta cagcgatttg
      961 taaaaatgct ttcagatatc tttgttccga ttataccagc catcgttgct ggtggtttat
     1021 taatggggtt aaataacatt ttgactgcga aagatttatt cttttcaggt aaatcattga
     1081 tagatgtata tagtcaattt gctggtttag ctgaaatgat aaatgttttt gcgaatgcac
     1141 catttacatt attaccaatt ttaattggat ttagtgcagc aaaacgcttt ggtggcaatc
     1201 cgtttttagg tgctgcatta ggtatgatat tggttcatcc atcgctaatg agcgcatacg
     1261 atttcccaaa agcagttgaa gcaggtaagg ctattccata ttgggatgta tttggtttgc
     1321 atattaatca agtaggttat caaggacaag tcttacctat gcttgtagca gcttatatct
     1381 tagcctcaat tgaaaaaggg ttacgcaaag ttattccaac ggtgttagat aatttgttaa
     1441 caccattgtt atctattttt ataacagcat ttctaacatt ttcatttgta ggtccaatca
     1501 ctcgacaatt aggttactgg ttatcagatg gtttaacatg gctttatgaa tttggtggtg
     1561 caattggtgg attaatattc ggattattgt atgctccgat tgttattaca ggtatgcatc
     1621 atagctttat agctgtagaa acgacattaa ttgcagatgc cactaaaacg ggtggatcat
     1681 ttatattccc gattgcgaca atgtctaatg ttgcacaagg tggtgcagca attgcagcgt
     1741 tctttattat taaacaaaat aaaaagttaa aaggtgtggc atctgccgca ggtatttcag
     1801 cattacttgg tattacagaa ccggctatgt ttggtgttaa cttaaaacta agatatccat
     1861 ttattggcgc tatcgttggg tcaggtattg gttcagcata tattgctttc ttcaaggtta
     1921 aagcaatcgc attaggaact gctggattgc ctggatttat ttcaatcaat ccagtacatg
     1981 caggatggtt acactacttt gttggtatga caatatcatt catcgttgct ataacagtta
     2041 ctttaatttt atcgaaaaga aaagcaaata aagaagttgt agaataaaaa aagaggtatg
     2101 acagcaatca aagtatcagt cattgcaaat gcaataggat gaaactttaa gctgtcatac
     2161 cttttaattt agttataatt tctgttttcg ccataagaaa atactaacaa ttacgacaag
     2221 aattaatgaa atacctacca caataatcca agatatataa ctatggtcat caattggcaa
     2281 cggcacattc ataccgaaga aactaaatac gagcgttggt aacgttaata gtaccgtgaa
     2341 aagtgttaat gtcttcataa tcgtattcat atcattagat aataaggaag cgtatgatgt
     2401 tgtgatactt tctaagatac gttgatgtaa ttctgttgtt tcgatggctt ggttattttc
     2461 aatgattaaa tcttctagta attcttcatc ttcttcaaaa cgtttaattg caggtaaacg
     2521 gaataacttt ttaataattg tatcgttacc ttttaaggca gctaaaaagt atactaaact
     2581 tttttcaact tccattaatt tgaaaagttg tttgttagta atgttatttt ttagttcttt
     2641 ctcaatacga attctacttt tattaagtaa tcgtaagtta cgattataat ggtcggcaat
     2701 agtagttaaa atttctaatg caaatctgct gtgaaatttt aaattaatat cacgcttagg
     2761 taaattttct aaaaattcat tttcagcgtc acaaactgtt acgataatac cgttgccaat
     2821 aataatgcct aatggaatcg ttacaaaaga tagaacgcta cgattagttg aattgacgat
     2881 aggtaaatca ataatgatta atgagtaacc agtatcttcg tcatattcaa tacgggaact
     2941 ttcttctgag tccaaagggt cacgtataaa gtcttcaggt atattatatt gttccataag
     3001 attttcaatt tcttctcggt ctggttctac tacattaatc catgacgctg tatgatctaa
     3061 tggtgttttt ataatatgtt tatctatatc ttcagaatgt ctgaatgaag taatcatgat
     3121 atatagttcc tttctaaagg atttaggaaa agcgagtatt acttaagtta gtgaatcaag
     3181 gtttgattta tgcaaacggt tttctgccat tattcttaat ggaccattaa aaacaatatt
     3241 atgcacatgt ttagccaatt catcaactga aatgggctgt ttgtccatta cccagtattt
     3301 aataattgaa attgttgctc cagaaacgta actgtaaaaa tacatctctg gaatgcctcc
     3361 gatagaatga ttgatactaa tataacgttg catattattt ttaatatgtt cgttgatttt
     3421 caattctaat tgactcgtgc gttccaattg taaaatagta tgataaaact ctaaattatc
     3481 atgaatatgc tggaaaatat ttttgagtat attatttaca aaagtattcg caatgtcttc
     3541 tattgaagaa ccgcgtaaca aatcaaattg agtatatgtc gttagttctg aaatatactc
     3601 atcttccata tctgcgagta acatatattt atcctcgtaa tgtaagtaaa atgtccctct
     3661 attgatatca gcgcgtgttg ttatgtcgcg aatggtaatc ttttctaaat ctttttcttg
     3721 tagtaattta gtgaacgctt gtttaatgga ggactttgtc tttctaattc gcctatcttc
     3781 tttcaaaaat aaatcaacct ccttgtgtta ttgaacaatg tgggtgtatt tgtctattaa
     3841 taaacgtatt gatgaacatt gtgtattgtt tattcccact tacatattat aatagacaac
     3901 atgcgaaata ttcaacacat tgtctaataa ggaggtaatt aataaaatga atatatttaa
     3961 aaataaatta ttatggattg caccaatagc aactatgatt atcttggtaa ttttttcttt
     4021 agctttttat cctgcatata atcctaaacc aaaagattta ccaattggta tattaaacga
     4081 ggacaaaggt acaacgattc aagataaaaa tgttaatatt ggtaaaaaat tagaggataa
     4141 attattagat agtgattcta ataaaattaa atgggttaag gttgatagtg aaaaagacct
     4201 tgaaaaagat ttgaaagatc aaaaaatatt tggagtagct attattgata aagatttttc
     4261 aaaagatgct atgagtaaaa cacaaaaagt agttatggat agtaaaaaag aagaaatgca
     4321 acaaaaagtt gcttcaggtg aaattccgcc acaagtagtt caacaaatga aacaaaaaat
     4381 ggggaatcaa caagtagagg ttaagcaggc taaatttaaa acgattgtaa gtgaaggatc
     4441 aagcttacaa ggttcacaaa ttgcatcagc tgtgttaact ggtatgggtg ataatattaa
     4501 tgctcaaatt acgaagcaaa gtttggaaac attaacgaat caaaatgtta aagtcaatgc
     4561 cgcggacatc aatggtttga cgaatccagt aaaagtggat aataaaaaac ttaataaagt
     4621 taaagatcac caagcaggtg gtaatgcacc attcctaatg tttatgccaa tttggatagg
     4681 ttcaatcgta acgtctatct tattgttctt tgcatttaga actagtaaca atatcgtcgt
     4741 gcaacatcgt atcattgctt caattggaca aatgatattt gcagttgttg cagcatttgc
     4801 tggtagcttc gtttatattt atttcatgca aggcgttcaa ggatttgatt ttgaccatcc
     4861 aaatcgtatc gcaatttttg tagcacttgc gattcttggt ttcgtgggcc ttattttagg
     4921 tgttatggta tggctaggta tgaagtcaat tccaattttc ttcattttaa tgttctttag
     4981 tatgcaactt gtaacgttac ctaaacaaat gttgcctgaa agttatcaaa aatatgtata
     5041 tgattggaat ccattcacac actatgcaac aagtgtaaga gaactattat acttgaatca
     5101 tcatattgaa ttaaatagta caatgtggat gtttataggg tttatgattt ttggcgctgt
     5161 atcaagttta gtttcagcta ttgttagaaa acatagcaca aaacgtactg aagtgccgtc
     5221 ataatttaat aaataggaat atagctatac aagacgtttg tagagagcgt catggtatag
     5281 ctattttatt tgtatagcac ttgatgtaaa gttattttag ctgtcatatt atgtgctaaa
     5341 ccaaattcag ttgtgtcata tcttcgcttt tttgtcactg ttataatagg tataatcgga
     5401 tataatgaaa aggtgaggga ggattcaaaa tgcgtaggtt attatattca tttctttttt
     5461 atatgatcat aggtttattt agtggctttt tctatagaga gctaacaaaa gcatatgatt
     5521 ttactggtac aacacaatta tcacttgtac acacacatac acttatttta ggtatgttta
     5581 tgtttttaat attattacca ttggagaagt tatttaaatt atcaagttac tacttattta
     5641 actggttctt ctatgtatat aacataggtg ttatcgttac tataggtatg atggtgacaa
     5701 aaggattctt ccaagtcact ggtaaatcat actctccaga agcgtttgca ggctttgcag
     5761 gtattgggca cacaggtatg cttgcaggtt tattgttgct atttttcctt ttaagacaag
     5821 caattcttaa agaaccacgt gatgagtctt ttaaaaaata atataactag aactaaaaaa
     5881 tatacacatc tttcaaagga acattgttgt ttcgtacttt gaaaggtgtg tttttatatt
     5941 gtgatgggat agttattgat aattcatggc taactatgcg atttatttta acaatataaa
     6001 tattaatagt ataatgtctt taaatgataa taattatcaa tttgagcgcg ttaatgaaag
     6061 aaatgaatat agagagtcat aggaggcaca taatgaaaga cgttacaatc attggtggcg
     6121 gtccgtctgg tttgtatgca agcttttatg ctggattgcg agatatgtca gtaaggttga
     6181 tagatgttca atctgaatta gggggtaaga tgagaattta ccctgaaaaa attatttggg
     6241 atattggcgg cattgcgcca aaaccttgtc atgaaatttt aaaagataca attaagcaag
     6301 gattatattt taaaccggaa gttcatttga atgaacgtgt ggtagatatt aggaaaaagt
     6361 cagaacgtca tttcgaagtt gaaacagaag cgggagaaat atatacatcg aaagcagtta
     6421 tcattgcaat tggtgcgggt attattaatc caaaacaatt agatgttaaa ggtgtagaga
     6481 gatatcaatt aactaattta cattatgttg tacaaagtta cagacgtttc aaagataaag
     6541 atgtattaat atcaggtgga ggaaatacag cattagattg ggcccatgat attgctaaga
     6601 ttgctaaaag cgtgacagtt gtttatcgaa aagaagatgt aagtggtcat gaagcgatga
     6661 aaacgctggt gacagattta aatgtgaaac tatgcccgaa aacacgtatt aaatacttgg
     6721 tcggcaatga tgatgaaacg catataagtg aagttgtttt ggaacatgtt gaaagtggtg
     6781 atacgcatac agttaaattt gacgatgtca ttattagtca tggttttgat cgttgtaata
     6841 cattattgag tgaaacatct tcaaagctag atatgcatga tgattgtcgt gttaaaggat
     6901 ttggtaatac gacaacaagt atacctggta tctatgcgtg tggagatatt gtttatcatg
     6961 atgcaaaatc acatctaatt gcaagtgcat ttagtgatgg tgcaaatgca gcgaatcttg
     7021 ctaaaacata tatccaacca gatgcgaatg cagagggtta tgtttcaagt catcatgaag
     7081 tgtttaaaga agcgaataag actattgtaa ataaacattt atactaggtt gatatattgt
     7141 gttatgaaaa gtggggtaaa taatggttaa agtgacatat gatattccga cttgcgagga
     7201 ttattgcgca ttaaggatta atgcaggtat gagtccaaaa acgcgcgaag cagctgaaaa
     7261 aggattacct aatgccttat ttacagtaac cttgtatgat aaagatcggt taattggtat
     7321 gggtagagtg attggcgatg gaggaactgt ttttcaaatt gttgatgttg cagttttgaa
     7381 aagttaccaa ggtcaaggtt acggcagtca aattatggaa catattatga agtatattaa
     7441 aaatgtatct gtcgaaagtg catacgttag tctgatcgca gactacccag cggataaatt
     7501 atatgcaaaa tttggattta tacctacaga accagattca ggcggtatgt atatgaaatt
     7561 ttaaaaatga aaaatcgtac cgataaacat actgagtttt ctacacagct taacttagtg
     7621 tgtttttgtt attctaagta atgatattaa aaaaatgtgt gttttatggt aaagttcaag
     7681 ttgatatgat atagaagttt cgactttgta actaagggag ttagaacaat gaaaatcgat
     7741 acattaagac aagctataat atattatttt gttgcacttg ttttatcagc tttaatgatc
     7801 tatttatttt taacaaacct tgagagagtt attttgtata gttgtgtatt cacagtcgtt
     7861 tattgggtaa tatatgcctt tgatgtatat agaaagagaa attaaatttt atttttagac
     7921 acaggctggg acataaatca atattctatg ctctacgaag ttatattggc agtatttgat
     7981 tagtgttgca agtatgatag gggcggagaa tacatgattc cctaaaatgt attaaagtaa
     8041 agtgctattt gatgaagtat cagaaatttt ataaaccaaa agaaattaaa aaagacatct
     8101 cgcataacga gatgtctttt tcatgaatac tatttatcta caccgaaatc gataacaatg
     8161 cggcctttat tttcatgttt aattactttg ttaagttgtt ctggaagttc atcaaatgta
     8221 ataacttgct tgatctcatg taaattttca ggctttaaat cttttgcgag acgacgccaa
     8281 acgcgctgtc ttaatttcat agcagtaaat actgagtcaa ttcccaaaat gtttacgcct
     8341 cttaaaatgt gagggaatac agaattagta taagtattac cggcagtcat accaataact
     8401 gcaattgatc cactatgatt taaacgcttt gtaacataat taataccttc gccaccaaca
     8461 gggtctacac aagcttgcca agttgatgat gcgagtggct ttttatgatc atcttcaaca
     8521 ggaagtcgat cgataacttc tttggcacca agttcaagta attgattgct aacatcttgt
     8581 ttacctgtac ttgcgataac tttataacca agttcattaa gcatgagtac tgctaaagta
     8641 ccgacaccac ctgaagcacc gcgaacgagt acaggaccat cttcaatatt cattccaact
     8701 ttttcaagtc tttcaattgc taaaccggca gtataaccag ctgtgccata tatcattgat
     8761 tcttctaatg ttaaagtatc aggaagcttg acaatccatt ctgattttac acgcgcatat
     8821 tcactaaaac cgccataatg gctgacacct aggtcataac tcgttacaat tacttgttct
     8881 cctttttcaa agcctggtgc ttcggattca acaattgtac cagctaaatc tattcctgga
     8941 atcataggat acgattttac gactgcatta tgatcttgag tcgctaaagc atctttataa
     9001 tttataccag agtaatgtac tttaatcagc acatctcctt taggtaaatc agtaggcgat
     9061 aattgtttga aagttggtgt cactttacca ctctcatctt tatcaattac aaacgcttta
     9121 aatgtttcta tcacaacgat acatcctctc tttttctctt ttgaaaatac ggccatttta
     9181 ctggaataaa tgtatcattt attgatgtta cacacaatca atacaatttg taattgattt
     9241 gtgttaatgt atttttaaat agttttggct gaaaaataac ataagtaata ttttgattcg
     9301 atcactgttt attggaggat ttatatggct gggataatca aagaaatttc aaaacaagat
     9361 gctagtgaat tagtggaatt agcaactaga acattttatg acacgtttgg ttcttactat
     9421 gatgacaaag attttgatca attttttaaa gataattata ctgtaggaaa atttacacaa
     9481 gagattaacc atgtagattc atttcattat ttttatcaag aagatggtgc gaatgttggt
     9541 tatataaaga tgaatattaa tagtgctcaa actgaagaaa tgggggagac ctatttagaa
     9601 gtgcagtgca tatatttttt gaaagacttt caaggtggcg gaagaggttc acaattgata
     9661 gcattggccg aaaaaattgc acaagaacat aataaacata aaatttggct aggagtttgg
     9721 gagcataatc ctcgtgctca agcattttat aaacgtcatg gatttaaagt ggttggtgaa
     9781 catcattttc aaacaggtga tgtaacggat acagatttaa ttatggaaaa agagttatag
     9841 cagtaacttc tgttgtcgat aaaaagaaat tgctgttgta atcattgaat atgtatagaa
     9901 atgtaaaatg tgtaataaaa ttgttgagta aaatgattaa atgtataaaa aacataaaat
     9961 tcgcaaaaat tagataataa tgattgaaat ttagggtata ataaaaagaa ccatagatga
    10021 tagttgttga tattggggga aataaaacac catattattg ttgacgtctt tggtaacaat
    10081 ccatgaaatg atacacatgg tagatatgta tttcaagctt attcaatgaa tatcgaatta
    10141 taggaggaga tatgtatgaa aaaattagtt acagggttat tagcattatc attattttta
    10201 gctgcatgtg gtcaagatag tgaccaacaa aaagacagta ataaagaaaa agatgataaa
    10261 gcgaaaactg aacaacaaga taaaaaaaca aatgattcat ctaaagataa gaaagacaat
    10321 aaagataata gtaaagacgt aaacaaagat aataaagata atagtgcaaa cgataaccag
    10381 caacaatcta attcaaatgc aacaaacaat gaccaaaatc aaacgaataa taaccagtca
    10441 aacagtggac aaacgactaa caatcaaaaa tcaagttacg ttgcaccata ttatggacaa
    10501 aacgcagcgc cagtggctcg tcaaatttat ccatttaatg gtaataaatc acaagcatta
    10561 caacaattgc ctaatttcca aacagcttta aatgcagcta acaacgaagc aaataaattt
    10621 ggtaatggtc ataaagttta taatgattat tcaattgaag aacataatgg taactataag
    10681 tatgttttta gttttaaaga cccaaacgta aatggaaaat attcaattgt aacagttgat
    10741 tatactggac aagcaatggt tactgatcca aactaccaac aataatgtta atgtaattat
    10801 gatgcaagtt aaaaaattaa aacggtaaac tctataatat gaattagggt ttaccgtttt
    10861 ttgcgtattt taaa
//
LOCUS       DAPIDM010000083        10870 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000083 DAPIDM010000000
VERSION     DAPIDM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.083"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            458..832
                     /locus_tag="RJE42_001810"
     CDS             458..832
                     /locus_tag="RJE42_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDV5877225.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(1092..1880)
                     /locus_tag="RJE42_001811"
     CDS             complement(1092..1880)
                     /locus_tag="RJE42_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877226.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLESHFLNVTKKIFEHSGHAPHIEEPEAFLSYYLNFLKSVS"
     gene            complement(2049..2765)
                     /locus_tag="RJE42_001812"
     CDS             complement(2049..2765)
                     /locus_tag="RJE42_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877227.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFILNFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTLDAMIHNPQSAQTFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(2927..3433)
                     /locus_tag="RJE42_001813"
     CDS             complement(2927..3433)
                     /locus_tag="RJE42_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877228.1"
                     /translation="MKKLLTASIIACSVVMGVGLANTSAEAASGNSIDTFKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNENGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(3569..4369)
                     /locus_tag="RJE42_001814"
     CDS             complement(3569..4369)
                     /locus_tag="RJE42_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877229.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(4353..5072)
                     /locus_tag="RJE42_001815"
     CDS             complement(4353..5072)
                     /locus_tag="RJE42_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5877230.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKIDNKGENYGSIY"
     gene            complement(5127..6077)
                     /locus_tag="RJE42_001816"
     CDS             complement(5127..6077)
                     /locus_tag="RJE42_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV5877231.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(6228..7115)
                     /locus_tag="RJE42_001817"
     CDS             complement(6228..7115)
                     /locus_tag="RJE42_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5877232.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQSSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(7445..8080)
                     /locus_tag="RJE42_001818"
     CDS             complement(7445..8080)
                     /locus_tag="RJE42_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDV5877233.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIRPSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKAELRKELLTLKGIGSETADVLLVYIFERIEFIPDSYTRKIYNKLGYENTKS
                     YDQLKKVITLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(8565..10226)
                     /locus_tag="RJE42_001819"
     CDS             complement(8565..10226)
                     /locus_tag="RJE42_001819"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV5877234.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNILLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVAAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIVKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(10223..10639)
                     /locus_tag="RJE42_001820"
     CDS             complement(10223..10639)
                     /locus_tag="RJE42_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDV5877235.1"
                     /translation="MDKKVSIYTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQATTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(10732..10840)
                     /locus_tag="RJE42_001821"
                     /pseudo
     CDS             complement(10732..10840)
                     /locus_tag="RJE42_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3551 a         1930 c         1412 g         3977 t
ORIGIN      
        1 tttttctttt ttagtgataa aagttcgaaa aatgggtata aatattagaa gaagttaact
       61 taaaagcgtt aagctatgac aatgaatcac tttagataca cattgaatat tgaaacattt
      121 aattgcgcta aatcgtttca ttaaataaat taccttgtac tgacgattaa attaaggtaa
      181 attataaaaa atgctgatct ttttgactaa tccaaatgct aacccagaaa tacaatcact
      241 gtgtctaatg aataatttgt tttataaaca cttttttgtt tacttctcat ttttaattag
      301 ttataattaa ctaaataata gagcattaaa tatatttaat aaaacttatt taatgcaaaa
      361 ttatgactaa catatctata ataaataaag attagatatc aatatattat cgggcaaatg
      421 tatcgagcaa gatgcatcaa atagggaggt tttaaacatg gcaattacaa aaatcaatga
      481 ttgctttgag ttgttatcaa tggtcactta tgctgacaaa ttaaaaagtt taattaaaaa
      541 ggaattttca attagctttg aagaattcgc tgtattgaca tacatcagcg aaaacaaaga
      601 gaaagaatac tatcttaaag atattattaa tcatttaaac tacaaacaac cacaagttgt
      661 taaagcagtt aaaattttat ctcaagaaga ttacttcgat aaaaaacgta atgagcatga
      721 tgaaagaact gtattaattc ttgttaatgc acaacaacgt aaaaaaatcg aatcattatt
      781 gagtcgagta aataaacgaa tcactgaagc aaacaacgaa attgaactat aattttgttt
      841 agcgcaattt ggtgaagttt gatagatgat acattctatt aaacttcctt tttttatgtc
      901 tttttttacc taattgttaa gaggttttgc actaatagca cttaatataa ggaagatagc
      961 cgtataacga gcagtatagc agtatatatc aaatacattt caaaaaaagc tgatttctat
     1021 caaataatga gtagtaatca gctttttcac attgcctaag gactttatgc cctaggttga
     1081 tatatcacat attatgatac gctttttaag aaattgagat aataacttag aaatgcttct
     1141 ggttcttcaa tatgcggtgc atgtcctgaa tgttcgaaga tttttttcgt tacatttaaa
     1201 aaatgcgatt ctaatttaaa atgattctta tctttaatca aaggatcatg ttccccatta
     1261 attaacaaag taggtacaca tgtacgtttt tttaaataca taggttcatc tttatgcaaa
     1321 attaatgaac gaattgccac cgcactatca tcctcagact taatgctata tattctttgt
     1381 ttttgatacc atttcatcgt cttctcttga tttctaaaca atttagaaaa taaaagaata
     1441 tacgcttcag acttattaaa acccgctata tcatcttgat attgaattaa caattgagta
     1501 aagctgtgta ttaagtcatc tttcttcgat gcaatggtag ttaatgattt tacacgataa
     1561 gcatatttat ctgtaaataa cttagcaact accccaccta aatcatgtcc tagaatatgt
     1621 gcctcatgaa tatttaactt ctccattaga atttttaaat cctcaacgtg atcgtttaaa
     1681 tcgtatgatt cacttttaga agacttgcca tgacctctta aatcgtaagt aagtacttta
     1741 tatgatgatg ctagttgatg ttgcaaatct tcaaatccag ctaaattacc atccaatcca
     1801 tgaagtaata ttataggatc ccctttacct tcaatttgat aagttaattg gatttgagga
     1861 ttaattgtga ctttattcat tgtgacacct ccctcaataa actatatacc caaccttcat
     1921 tctatgtacg cttccatttt ataaacaatt tttttaaatt atttataaat taccaaaagt
     1981 tattccaaca ataaaaaaac tattcatctc aattaataca attaatcaag tgaatagttt
     2041 tatcatattt ataatcttgt acgtgaagat aatctaattg tgaaatatat aactagaact
     2101 actaataata tccatataat taatgaaaaa gtctgggctg attgtggatt atgaatcatt
     2161 gcatctaagg ttttttgaca aaataataag ccgattcccg aaaactcaag tcgcttataa
     2221 aaaatgccgg caattactaa aataatacca atgaaaaaga aaaattgatg tccaaaattt
     2281 gtaattggta taacgcccca tgtaatatta aatgcttgta atataattaa taacagggat
     2341 atgatagcga taattgtacc cataatcatt tctattttat tactacgcaa gtaattcttt
     2401 tgtgacaccc ttaggtatac aacaatagtc attggaaaaa tcactaaaca ataaaataga
     2461 ctcattccag taatagcttg aaattgaaca tactgtttat catataaata ggatatgaga
     2521 atgaaattta aaataaagaa aataactgga ttcatttgta aagcaaacaa attcctttga
     2581 atcattaata tgatttgtgc tggagatttt cttttatatt caatataatc ttgatctttc
     2641 ttttcagaat tttttatatc agtaattact ttctctttaa tatcatcaat caaatgcggt
     2701 gataaacctt tatgattaaa gtaatcttct atattttcta gtaaaatttt atcgttaata
     2761 cgcattggta gtactccttt aaaatgaaac atctttattt attgtattgg tttaatatcc
     2821 ccttcgcaaa tcataaaaat taatattcac tgtattatgc catgtaataa aataaccgac
     2881 aagtattgcg atttaacttg tcggttaagc acgattaatt taattattat ttttccaaat
     2941 caatacgata taattttatg tttttatctt ttggtgctga ggcaaattga taagtcgttt
     3001 tgcctttttc aatatatcca aaattaccag caactttgct atattccgta tgtgttactg
     3061 ctttgccaac ataattttta acagcttgat atgttggacc attagcatca ttatacatca
     3121 ttgaaatatg agttacttta ccattgtttt tcttaccatc agtagttact aataacatac
     3181 cttttttagt atggaattcg taataatgtt cagttccatt ttcattgtaa gaatacatcg
     3241 gatttttgta cttagttaaa acatctttaa tagattcgcc aattttcaca ttttctaatg
     3301 actgatcacc cttaattaat tgtttaaaag tatcaataga gttgccactt gctgcttcgg
     3361 cactagtgtt cgctaagcct actcccatta caacagaaca tgcaattata cttgccgtta
     3421 gtagcttttt cataagcact ctctcctttt atttatatcg tcttgtgctc acaaccatta
     3481 tacaacaatc atttctaaaa caacagtcat attacaatta tattacaaat aataattact
     3541 acttttatat ttcacttatc actaaaaatt aaacatgctt ttcatcgtga tgtttgttga
     3601 taaatgcagc tgtatcttcg acgaaccttt cttgctcttc aacaaatggg aatgcacttg
     3661 attcttgata tacttcaaag tccgcatttt ggattaaatc agctacttct ttagcttcta
     3721 atcttgttgt tctttcaccg aattctcctg caataatttt tgtcggtact acgacatttc
     3781 tataagtttg agaaatatca gcgtttttga atacctcttt aactgcttga atttcttctt
     3841 tagttgatat ttcatttgta tctacgacat gtttaaggaa tcgattcatt tttctcggac
     3901 gataatactt acgcttattt aaaaatttat cttgtttttc aggatcccaa tttcgaataa
     3961 tatgagcata ttttctaaat aaacgttctt ccggtaattc accttcaata gatgttggat
     4021 ttaccaacat aagtgatgat gtaaattcag gataacgtac tgaaatatcc gcaccaataa
     4081 ttccacccat ttcatggcat acaaatgcaa cttcttcaat gtatagatat ttaagtaatt
     4141 caacaatatc atcagaaaaa tcttttattt caatgtgacg aggtttatca gaatagccat
     4201 gtccacgtaa atcaattaac acaacttgaa atgattttgc taattctgct gctaatttat
     4261 taaacacaga ataattatca agtacagtat gaatcaatac gataggatag ccttcaccta
     4321 atgtactgta atgtatcgat gttccatctt ttctagtaaa tagatccata attttctcct
     4381 ttattatcaa tttttattgg taaaatggtt aataattctt ccactgttgt aattgtaaaa
     4441 tcaacttctt cttcgagtgg ttcaatttca gcatcttctt gtttatacca tacactaacc
     4501 atgcccatag ctcgtgctgg agctacatca tttaacgcat catcgccaac atacataatt
     4561 tgctcaggta atacccctag ttgatcaatc atatcttcaa aaattttagg atgtggttta
     4621 cgaaaaccaa ctgtttctga tgttgataaa taattaataa catgcatcaa accaagtgaa
     4681 tgtaatcgaa attgtttaat cttagattta ccatttgcga taacaccaat ttgaaagcct
     4741 ttttgcgata gcttttctag tgtatacaaa gtatcgtaat aaggaaatac ataacgataa
     4801 aaatgcattt caaaatcgtt aaataagtct ttccaagtta acctatctac atggaaacgt
     4861 ttaatgattt ctttatataa atcaggttta tcattatctt catcgtcatc tagctcaata
     4921 aatgcttttt taaaatctgc cagttgaaca tgaattaagt agtcatgaaa tcgttcatat
     4981 tgctcttcga taaatttctc tcgagattta actctgtcca acaacgtacc ttctaaatca
     5041 aatacaactg ctttaatttg attcaaatcc atcgcttatg ccctctttat gtttatagtt
     5101 atttttattt tagcaaatgt catgtgctag tgtaaccgtt tcgactgaga tattagcatg
     5161 taaaataaca ctggtatacc aattaatgca atgataatac ttgccggtat ttgtactggt
     5221 gacaatatat ggctaccaat aaaatctgcc ataaccatta tttgtcctcc aattacaata
     5281 ttcaaagcca attgtcttcc taatgattgt ttccactgcc attttctaat gagttgtggt
     5341 atcaccatac caatgaatcc aatgatgcca acatatgcga catttatcgc cgttatcata
     5401 gaagctatac ataacgcaat atatgtaatc aattgaacat ttaagcctag ggactgactt
     5461 tttagttctc ctaattgtaa taactttagt tgattaagaa tgataaatat accaaacagt
     5521 gcaatgataa atgtgattgc tattatggat acatttgagt attctgcatc accaaaacca
     5581 ccaaacagat aattggcaat tgtatttaat ttgcgaggtt ttaataaaat caaaaaatat
     5641 agaagtgaat tgaataatgc accaatcatt aaaccactta atattaagat tctaactgga
     5701 tagccttggc tcaataccga cgtaatgact aatacagtta ttaatgttat taaactaaat
     5761 gttattgaaa atacaggaat ccataacgtt gttaatccta aaaataatgc taatcctgaa
     5821 ccaaatgtag caccgcttgc caatcctaat gtaaagctat ctgccaatgc attatttaaa
     5881 actgtttgaa atataaggcc tgcaacagtt aatatcatac ctgtcaacaa tgcttctaat
     5941 aatctcggta atctaacatt gattaaaata gactggttaa atacatcatt aatatccccc
     6001 aactgccaaa atagatatat gctagttgtt ataatcaata tgactatcca gctcaataat
     6061 actttattaa atgtcatagc attctctttt ctttcatcaa taattacatt cattatagca
     6121 atcacatact cgcgttgcta tcattcacta aatatatttt aaaaacccct tatctcaaac
     6181 atgtaatttt tattacatgt agcaataagg ggtataatta gaatggttta ttttctataa
     6241 attgcatctc ttaattcttt taatccttca tcaatacgtg gacctggtct tgatacttca
     6301 tcaccattta caacttcaat acgtgtattc ttgacagcat taattttatt gaatccacct
     6361 ctttttttga tgatatccat ataatctgat cttgtcttag cttctgtcga aattaatata
     6421 tctgggttct ttttaataat actttccttc gttacagggt tccaaccatt aatgtcacta
     6481 taaacatttt gggcttctaa tttttctaac atatcattga aaaatgtatg cttccctgct
     6541 gtatatattt caggctttga tgaaacctca ataaatactt ttgatttttt atgatgagca
     6601 ggaattgaat ctatgacttt atcgatatta tctttagttt cctcaacaag ttgttcagcc
     6661 tgcttatcat gatgcgttaa tttcccaatt tgcttaaatg tgttgtaagt ttcatcaatt
     6721 gattgtgcat ctttaacata cactactttg atgccttgtt tctctaatga tgataatact
     6781 ttattagcag ttgccttttg cgactcatgc gcaagaatta gatctggctt tgcctttaaa
     6841 agttcctctt tatttagatt caaagcatca aattgtttct tacccttttt cacatctttt
     6901 ggataatcat caaccgttga aacaccaact atgtatttac ctaatcctaa ttcatataaa
     6961 atttcagtat tactaggcat taacgaaaca attctatggt acggcgattt agacgagcca
     7021 ttatcatcgc tactttgttt atcattatct ttcataccac agccactcag gacaagcata
     7081 aaaatcaata taaaagcaat taacgatttc ttcacactat cacatctcct ttttaaagcc
     7141 tcttttcgct ttagcgatta gagggattta tgcagttgtg cggtagcatc caactgcgtt
     7201 cctttaaagc ctcttgaact ctagtgatta gaggctttta tgcagttgtg cggtagcatc
     7261 caactgcgtt cctttttaaa gcctcttcac ctttagcgat tagaggcttt tatgcagttg
     7321 ctgcacaaac aaacaacttt tttctttata tgcttctatt tattatataa gtccacatac
     7381 taaaagaaaa tagtgaatta ttttttatat ttcatataaa ctcatctatc taacttcaca
     7441 gcgtttactt tttaaagtaa ggttctaaaa aatcataatt ttttatatct ttatctctaa
     7501 agtaatgttt accaaataca tctaacagag catgaaattc attagcatct tgatttgtaa
     7561 aatgatttgg taatgtgatt acttttttta attgatcata acttttagtg ttttcatatc
     7621 ctaatttatt atatattttt cttgtatagc tatctggaat aaattcaata cgttcgaata
     7681 tataaacaag taagacatct gctgtttcac ttccaatacc tttcaaagtt aataattctt
     7741 ttcttaattc agctttatat cgctcattaa tctcttgata attgaaatga tgtcgtgcta
     7801 accatgttaa taatgtttta atcgtcagtg atttactttt ataaaagcca cttggacgta
     7861 tcaatgattg taacgtttca ataggtagtt ctaatatatg atttggatta aaatgcgtgt
     7921 gttctttaat ctgattcaat gcaatttctg catttcgcca tctagtattt tgaactaaaa
     7981 ttgcacctaa catcatttca atgtcattat cagcaggcca ccaattttga ggtcccatat
     8041 gcctatataa caatttatac aattcatcag ttcctaacat ttacatcctc cttttagagc
     8101 cccttgagct ttagcgatta ggggctttta tgcagttgct gcgcaagcaa accaactgta
     8161 ttcctttaac tccccttgag ctttagcgat taggggctct tttgcagttg ctgcgcaagc
     8221 aagcctactg tattccttta actccccttg agctttagcg attaggggat cttatgcagt
     8281 tgctgcgcaa gcaaaccaac tgtattcctt taacccccct tgagctttag cgattagggg
     8341 cttttatgca gttgctgcgc aagcaagcca actgtattcc tttaacctcc cttgagcttt
     8401 agcgatttgg gcttttatgc aattgtgcgc aagcaaacca actggattcc tttaacctta
     8461 ttcacatact aagcattccc caatatgtca catacctaat aaaaaaacac tccaacacaa
     8521 agtatctttc gagaaactct acgttggagt gtatataaat gttcttacat tgattcaggt
     8581 gcgcttacac cgaccattgc caatgcattt ttcaatgtaa ttctgaccgc ttcaatcata
     8641 gcaacatgtg cttttgtttt tacaatatca tctgttaaca ctttttcagc attatagaat
     8701 ttatggaaat gagaagctaa atcttgaata taattagtaa ttctatgtgc tgatctatgc
     8761 tcagcagcac tttcaattgt aggttcgaaa tcagctactt ttttcaacaa ttcgatcgct
     8821 ttttcatttg taatcgtagt aaaatcattc gcagcagcta cctcaattcc ttgctctttc
     8881 gcttgtttta aaattgaaca aatacgcgca tgtgcatatt gagcatagta aactggattg
     8941 tcttgagatt gctctttcgc taattccata tcaaaatcaa agtgactatc aggactacgc
     9001 atagttaaga aataacgtgc agcgtcaacg ccaacttcgt ccataatttc tcttaatgta
     9061 atggcattac cagtacgttt actcatcttc acttctttac cattttccat taaacgaacc
     9121 atttgcatga tttgaatttc taaacgatta ctatctacac caaacgtttc aagagatgct
     9181 ttcaaacgat taatataacc atgatgatca gcaccaaata aatcgattaa aatgtcatta
     9241 ccacgtttaa ctttatcgaa gtggtacgca atatctggta agaaatacgt atatgtaccg
     9301 tcatttttaa ttaatactct gtctttgtcg tctttaaaat cagttgtacg taaccatgta
     9361 gcgccatcag cttcatacgt ataacctaat tctttcattt ttgctaaaac ttcaagaatt
     9421 tcgccttttt catataaaga tgtttcacta aaccaattat caaaatgcgt attgaactct
     9481 gctaaatcat ttttcagttt agccatttcg tattctacgc ctaattttct aaattctttc
     9541 aaacgtgctt cttcagaata atctttaatt tcagggtgtt tctctgctaa atctttacct
     9601 atttcaataa tatctttgcc attatagcca tcttctggca ttgaataact attgtcacct
     9661 aaagcttcaa agaaacgtgt ttcaatcgaa cgcgctaagt tagtaatttg attaccagca
     9721 tcattaatat aatattcacg tgttacatta tagccagctg cagttaaaat attagctaaa
     9781 gcatcaccaa ctgctgcatt tctagcatga ccaatatgta aatctcctgt agggttagct
     9841 gaaacatact caagcaatat attttgacct tttgattcat ttacatgtcc aaattgatca
     9901 cctttttcaa ttgcttcagg aataattgct gttaaatact gattatctaa gtaaaaatta
     9961 atgaatcctg gaccagcaat gtcaatttgt tttacatgtg ctttttcagt atctaagtta
    10021 tcaacaatcg cttgagcaat ttcacgagga ttacgctttg caatcttagt cagtaccatc
    10081 gcaatattag tagcataatc tccatttttt gtatctttag gaacttcaat tttaatatca
    10141 ggaatctcat ctgctaatcc tgctttgtta atacttgctg caatttcttc tactaatgtt
    10201 tgtttcactt gatcaataat attcattcgc ctatctcctt atagttaatt tcatattgat
    10261 aagaacccat tttttcatta tcttgatata attcataatg aatctttaac ttgccaccgt
    10321 ctccacttac gaaatgtaaa atgcgtaatg ttttaacttc tagtggaatt cgtccagccg
    10381 atatatcgta aaaagttgtt gtcgcttgtc cttcaacaaa atgcaaattc atattaatgt
    10441 cgcctttacg aatcaactta acgctatcat catccacttt tattgtaaca ttaactgttg
    10501 catcctcaat ttgttctaca taccgaataa agttagattg cctttgttgc caagttcctt
    10561 cagtagtaaa ttcaaatttt tctttttcgt tgtgctgttt caacacttgc tttgtataaa
    10621 tacttacttt tttatccaat ttactactca ccttaatctt cgcataatag aaatattata
    10681 gcataaaata aggtcatctt gttatgcctt cttaaagcta aataggttca attaattaac
    10741 aattccatac ttaatcaacg cttgatatat gtaagccatt ttataaaata caaacaacac
    10801 ctcaaaacca aaaaaacacg agattttcac tatctttcat gaaaacctcg tgtttgatta
    10861 taatgatagt
//
LOCUS       DAPIDM010000084        10789 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000084 DAPIDM010000000
VERSION     DAPIDM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.084"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(230..928)
                     /locus_tag="RJE42_001822"
     CDS             complement(230..928)
                     /locus_tag="RJE42_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDV5877236.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(945..1934)
                     /gene="floA"
                     /locus_tag="RJE42_001823"
     CDS             complement(945..1934)
                     /gene="floA"
                     /locus_tag="RJE42_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDV5877237.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1952..2635)
                     /locus_tag="RJE42_001824"
     CDS             complement(1952..2635)
                     /locus_tag="RJE42_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV5877238.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(2879..3055)
                     /locus_tag="RJE42_001825"
     CDS             complement(2879..3055)
                     /locus_tag="RJE42_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV5877239.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(3348..4694)
                     /gene="mtaB"
                     /locus_tag="RJE42_001826"
     CDS             complement(3348..4694)
                     /gene="mtaB"
                     /locus_tag="RJE42_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDV5877240.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(4701..5453)
                     /locus_tag="RJE42_001827"
     CDS             complement(4701..5453)
                     /locus_tag="RJE42_001827"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV5877241.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEVADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(5455..6393)
                     /gene="prmA"
                     /locus_tag="RJE42_001828"
     CDS             complement(5455..6393)
                     /gene="prmA"
                     /locus_tag="RJE42_001828"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV5877242.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQRIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(6397..7536)
                     /gene="dnaJ"
                     /locus_tag="RJE42_001829"
     CDS             complement(6397..7536)
                     /gene="dnaJ"
                     /locus_tag="RJE42_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV5877243.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(7672..9504)
                     /gene="dnaK"
                     /locus_tag="RJE42_001830"
     CDS             complement(7672..9504)
                     /gene="dnaK"
                     /locus_tag="RJE42_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV5877244.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(9573..10199)
                     /gene="grpE"
                     /locus_tag="RJE42_001831"
     CDS             complement(9573..10199)
                     /gene="grpE"
                     /locus_tag="RJE42_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV5877245.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFKSGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(10231..>10789)
                     /locus_tag="RJE42_001832"
     CDS             complement(10231..>10789)
                     /locus_tag="RJE42_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HDV5877246.1"
                     /translation="IRANPNLVIMVIVFSSGHVEHVHLASDMPFSNDKLNTISNFVTN
                     KLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMNNHISNQSNSIYMGGKVKLIDALNE
                     SNVSSIQPILQYIESNRIAELLQDISSPNINVKIGDEIDDSLSDISIVTSQYHFDETL
                     KGQIAVIGPTAMHYQNVIQLLNRIW"
BASE COUNT         3146 a         2094 c         1494 g         4055 t
ORIGIN      
        1 ttgattgatt taaacatctt tgcattgact tactcatttt tctttaattt aataaaatca
       61 tttatacttt tcaccttgtc ataaaagcag attcaatttt agactaatca attttttgtt
      121 gtaatttaaa taactcatat ttctcgaaag attctctttg tttttcaatt tttcttaacg
      181 taaaaaaaca agcaactacc attacgatag tgcttgtttt caaaaaattt tataattgtt
      241 ttggtttagc taaaatttct gaccatatca taccattgat tacttcatct ttatcaaatt
      301 ggaacgctga tttagtcaaa tctttttcga cattttgaga attaagcaac tgctttaatt
      361 tcaaacgttt tgtacgttca gataagtatt tatcctcaat aatatctcta gcacgttttt
      421 ccattttagc aatttgcttt tctttttctc tatcaatgtc agtacgaatc gttttaatat
      481 catctctaag tgatttttct aattgacgtc taatttcgtc agaattatct tgtctagaag
      541 gtctatgttt ctgctccacc ggttctgctt taggcactgt aattggtttc tctgtcatcg
      601 gttttgtttc ttgttgtttt ttaggtgcca taggttctac aacaggcttc gatttaggct
      661 cttcctttgg aagttcatcg aataaaggtg gtaacgtatc atcatatttt cgtttcgatg
      721 atttcttttc ttcttcattt aattcttcac ttatttcttt aaacgttcgc tcaatttctt
      781 caaaaaagcc accttttttt ggttcattat cggtagatgt tttttgaggt ggcttttgat
      841 tttgtctatc tttatgacta ttttcgcgca tagtagtaat gatagaaatg atcactgata
      901 tgacaaaaat tagaataccg acactcattt aatcacctct cgacttaatg ttcaggtgac
      961 tcatcatcac tttgatcagt tcgtttatta attgcatttc tcatgcctgt atcagcttcg
     1021 atatttttca aattataata atctttaaca ctgatattac ctgaacgtaa tgcttcagcc
     1081 atagccaatg gtacttcaga ttcggcttca actactttag catgcatttc ttgtacacgc
     1141 gctttcattt cttgctcagt tgctacagcc atagctctac gttcttcagc ttttgcttgt
     1201 gcaatatttt tgtctgctaa tgcttgttca gtttgtaagt ctgcaccaat atttttacta
     1261 atatcaacgt cagcaatatc aattgataaa atttcaaatg cagtacctga atctaaacct
     1321 ttgcttaata ctgttttaga aatattatct gggttttcaa gtacttctgt atgatgctta
     1381 ctagaaccaa ttgttgaaac gataccttca ccaacacgtg cgatgattgt ttcttcacca
     1441 gcaccaccaa caagtcgagc aatattagct ctaactgtga tacgagcttt ggctttcact
     1501 tcaataccgt tcattgctac acctgcgata aatggtgttt caatgacttt aggattaaca
     1561 gacatttgaa ccgcttctaa tacgtctcgt cctgcaaggt caattgcagc agcacgttcg
     1621 aaaggaagat caatgtcagc acgttgtgca gcaatattag cgtcaacaac tctgtcaaca
     1681 tttcctcctg ctagataatg cgattctaat tggtttgttg ttaatgctag tcctgctttg
     1741 tgcgctttaa ttaatggcgc tataactttt cttggagata cacgacgtaa acgcatacca
     1801 accaatgtac ctataccaac atgaacgcca gctgctaacg ctgaaatcca taaaccaatc
     1861 ggtacaaatg agaataaaat aagtaatgca actactataa taactgctat tacgataaaa
     1921 cttaaactaa acatggtatc gctcctttta attaatctac ttccctcaca actactcttg
     1981 ttccttcaac ttcaaggatt tttaccgttt tattgcgtaa aataaagttg ccatctgaaa
     2041 cagcatcaat acgttcattt tcacaaaaaa taatccctgc aggtcgaaga tctgtaactg
     2101 tttgagcagt ctttcctacg aggtgcgagc ggttatcatg agaattgtaa cctgactcag
     2161 aattagttga atcttttaag ataactttat ccaaaaacgg aatctttctg ttgaaaatct
     2221 tcactaatat cacccattct acaatcgtta aaatcaaggc aacgataaca ttcgcaagca
     2281 taaatagcaa attatcaccg agcgttgtta tgcttatagt tatcagtatc atgccaataa
     2341 taccaattac tgcaccaact acaaataatt caattacaac taatataacg ccaattgaga
     2401 atattaagat agaatgcata ttgacatttc cttggattag aaatcccaaa aataaaataa
     2461 gtaatgataa tgtggcgata ataccagctg cattgatttt tttagagtaa agttgatata
     2521 caaatcctaa gaatgtcaaa caggttaata ttaacgtaaa aataggttga acaattatat
     2581 tactaacttg ttcaacccaa gtatctccag ccgttgattc caagatagtt gtcatttgta
     2641 aaaaattatt ataactcaca atttcacctc gccctcacat taattataca tgaaattgaa
     2701 tatactttat agttataaga acctatagaa tatcaatttg attatttata aactatataa
     2761 cttttttaaa ttaaaaattg acaacaaaaa aacatcacat attagtgtca gacactaact
     2821 tgtgatgttt attttatata attaatattc gtgttgaggg agtcaacaga ggtattaatt
     2881 atttgaattt acgtttacgt gcagcttctg atttcttttt acgttttacg cttggttttt
     2941 cgtaaaattc acgtttacgt acttcttgga ttgttccact tttagaaact gaacgtttaa
     3001 atctacgtaa cgcatcttca agtgattcat ttttacgtac tactgtttta gacatctgta
     3061 tttccctccc tccaaatatc aacggaactt tatagtcaat tgcacagtaa aactatgcat
     3121 ttataagtat aatatatctg taatttatgg tcaattagta aattgttttt tatttgaaac
     3181 atatttcaca tcaaaatcac aaagactttt agattttgtt ctaaaaatct cttaataatt
     3241 tttttaatga gaagagttgc ttatatagta aattgtgaag ccgttaaaac gacgttacaa
     3301 aacctatatc ttaaatacgg aaccttatgg tatgaatcaa ggaatactta aactaaaact
     3361 tctctatcag atttatttgt tgcaaaatca acaactttaa ttgcttgccc ttcatttaat
     3421 ggataatttg cttgcgtaat tttaactttt acaatttgac ctatgagtga ttcgtcacct
     3481 tcaaattgta ctttcatata attatctgca tatccaacta atgtaccttc tgtgtcaccc
     3541 tgttcctcag gaattacttc aagcacatct tgatcaaatt tagacgcata taactttccg
     3601 agttgattgc ttagcgtaat taacttatgc acacgttcat ttttaatttc ttcatcaatt
     3661 tggtcatcca ttcttgcagc tggcgtgcca attctaggag aataagggaa aacatgcagt
     3721 tcagagaact tatgctttac gataaaatca tatgtttctt ggaactcagc ttcagtttca
     3781 cctgggaaac caacaattac atcactcgta actgccaagt ctggtaaagc tttatgcaat
     3841 tttgttaatc gttctgaaaa tctatccatt gtatacttac gtctcatacg ttttaatact
     3901 gtatctgaac cagattgtaa tggaatatgc aaatgacgca caacttttgt tgaacgttct
     3961 aaaacgtcaa tcacttcatc tgtaagttga cttgcttcaa ttgaagaaat tcgaattcgt
     4021 tctaatccat taatcgtttc aagatcacgt aataattggg ccaagttata atcttttaaa
     4081 tcttgaccat atccacctgt atgaattccc gtcaatacaa tttccttata tcctgaattc
     4141 actagttgcg tcgcttgttc aactactttt tccggatctc ttgaacgcat taagccacga
     4201 gcccatggaa taatacagaa tgtgcaaaag ttgttacaac cttcttgaat ttttaatgac
     4261 gcacgtgttc tatctgtaaa ataagggaca tctaattctt catatttacg atttttcatg
     4321 atatttccaa caccattaat tggttggcgt tctttacgga attcatcaat gtaacctaat
     4381 agtttatgtc tatcttgtgt accaactact acatcgacac caggaatttc cataatttca
     4441 gctgatgaag tttgcgcata acaacctgtt acacagatta cagcatcagg attttgtctt
     4501 attgcacgtc taataatttg acgacttttt ttatcacctg tattcgttac tgtacaagta
     4561 ttaataacaa atacatcagc attcgcttca aagtcaacgc gctcatagtt tgcttcttta
     4621 aataattgcc agattgcttc agtttcataa tggtttactt tacaacctaa tgtgtgaaac
     4681 gcaactgttg acataaatat tcaccccatt aattcttttt cataacttat tgcacttaac
     4741 gcatacaatg gcgcagtttc tgcccgtaaa attctcggtc caagaccaac aactgtacta
     4801 gtattactaa ataatgaaat ttcattttct gacaaaccac cctcaggacc aaaaatcatc
     4861 aacactttat cctgagcctt gaattgttgt aaagtttgct tgaaattgct taactcacca
     4921 tcttttgctt cctcttcata tgcaataaga atatagtcat aattatcaat agtatcacaa
     4981 attaatttta aattcgactc gaattgaata gatggaatca ctaaacgata gctttgttca
     5041 gcagcttctt taattatttt ttgccaacgt tctatctttt tggcaacttt tgcctcgttt
     5101 aatttaacaa ttgaacgttc catgctaaca gctataaacg atgaagcacc caattcagta
     5161 gctttttgta gcaaccactc atatttgtca gctttaatta gtccactgca aatcgtaaca
     5221 tcaactggca attctgtatt aatattttgt ttttctttta aatcaactgc aattttatca
     5281 cttgttatat cagcaacttc acataaataa actgtttgat cattaaaagt taaaataatt
     5341 ttactaccaa catcatatct cattacattt gttatatgat gaatatcttc tttttttgta
     5401 ataaaaaaac gctgacttac atcagcgttt tggtctatga aataacgttg cacattattc
     5461 actcactttc tggccaacaa gacaaaccca accattgtca tgttgttctg aaataatttt
     5521 aaaacctaca cgctccatat gtgactgtat accttcatac ttctctttta taataccaga
     5581 agtaataaaa taaccgcctt catttagagt attataagca tcttcaatca tttcatcaat
     5641 aatatgcgct aaaatatttg ctattacaat atcaaatttt tctgtttcgt ctttcaataa
     5701 gttacctgga acagcttcaa ttaacgtttc acaatgattt cttctgaagt tttctttagc
     5761 tacactcact gccatttcat caatatccaa cgctttaata cgttttacac cgattagatg
     5821 acttgcaata cttaatatac ctgagccagt accaacatca attactgaat gctgtggcaa
     5881 tacatatgtt tctattgcct tcaaacacat acttgtagtc ggatgatcgc ctgttccaaa
     5941 agccatacct ggatcgagct caatgcaaag ctcttcatcc gcttctttag catatgtttc
     6001 ccaactaggg actattgtga acttctttga cgcccggaat ggatggaaat agtttttcca
     6061 ttcattttcc caatccgttt ctgcaatgat ttgctcactg aattgaacgt tatgttgatc
     6121 aagttcatct aaatttaata actcatcttt aattcgctgt cgcaacttat catcataagt
     6181 catctcatta aaataggctt tcaatcttac tcccttatct ggataatcct cttttttcaa
     6241 agcgtaaatt tcaccgtatt tatcttctgg ttggttaatt aaatcatctg aatcttctat
     6301 cacgacacca tttgatccat gattttcaag tatattggta gccaattcta ctgcttcatg
     6361 attaataata attgaaagct ctgtccagtt catactttat tctcccttaa aaaatctttt
     6421 tgctctatct ttaaaattcg aaggttgctc attaatttct tcgccattta attgggcaaa
     6481 ttctttcatt agttcttttt gtctatctgt taatttagta ggcgttacta ctttaatatc
     6541 aacatataaa tctccgtatc catagccatg aacatttttt ataccctttt cttttaagcg
     6601 gaattgctta cctgtttgtg taccagcagg aattgttaac ataacttcat tatttaatgt
     6661 tggtattttt atttcatcac ctaaagctgc ttgtgggaag ctaacattta atttgtaata
     6721 aatatcatca ccatcacgtt taaatgtttc agatggttta actctaaata ctacgtataa
     6781 atcgccagca ggtcctccat tcacgcctgg agagccttca ccagctaatc taatttgttg
     6841 ttcattgtcg acaccttcag gtactttcac ttctaattta actgttttat tttcagtacc
     6901 ttttccgtga catgttggac aagcttcttc aaattcttga ccacttccat tacatttagg
     6961 acaaacttgt tcagtacgaa ctctacctaa aattgtattt tgttctacag ctacatgacc
     7021 agcgccatta cagtaactac aagtcttttt acttgttcca ggctttgcac catcaccatg
     7081 acatgtttcg catgttacat ctttacggat tgaaatttct tttgttgtac caaataccgc
     7141 ttcttcaaat gtcaatgtca ttgtatactg aagatcatca cctttttgcg gtgcatttgg
     7201 atctctttgt ctgccgccac cgaagaaaga gctaaagata tcttcaaaac cgccgccacc
     7261 gaagccacta aaaccaccaa agtcagagcc attgaatcct tgtccaccaa aaccttgcgg
     7321 accgtcatgt ccaaattgat catagcttgc gcgtttatta tcatcactta aaacttcata
     7381 ggcttcagaa atttctttaa acttttcatc tgcaccttct tctttgttaa tatctggatg
     7441 atattttttc gaaagctttc gatacgcttt tttgatttca tcttttgaag catctttact
     7501 aatgcctaaa acttcataat agtctctttt ggccacagct atctctcctt ttcttaatta
     7561 actcatatag tttaacgtaa tatgtcatac tatccaaata aaaagccaaa gccaatgttc
     7621 tattgacttt gacttttcag atcatgacaa cattctaatt gtattgttta attatttttt
     7681 gtcgtcgtct tttacttctt taaattcagc atcttctaca gtgctatcgt tgttttgacc
     7741 agcattagca ccttgtgctt gttgttgctg ttgagccgct tgctcatata cttttgctga
     7801 taattcttga atcacttttt caagttcttc ttttttagat ttaatatctt ctatatcttg
     7861 accttctaaa gcagttttaa gagcgtcttt tttctcctca gcagattttt tatcttcttc
     7921 accgatattt tcgcctaaat cagttaaagt tttttcaact tggaatacta ggctgtcagc
     7981 ttcgtttctt aagtctactt cttcacgacg ttttttatct gcttcagcgt taacttcagc
     8041 atcttttacc atacggtcga tttcttcgtc tgataatgaa gaacttgatt gaattgtaat
     8101 tctttgttct ttatttgtac ctaagtcttt tgcagttaca tttacaatac cgtttttatc
     8161 gatatcaaac gttacttcaa tttgaggttt accacgttca gctggtggaa tatcagtcaa
     8221 ttggaatcta ccaagtgttt tattatccgc agccattgga cgttcacctt gtaatacgtg
     8281 tacatctact gatggttgat tatctactgc tgttgaatag atttgagatt tagatgttgg
     8341 aatcgtagtg ttacgttcaa ttaacgtatt catacgtcca cctaaaattt caatacctaa
     8401 agatagtggt gttacgtcta ataatactac gtctttaacg tcacctgtga taacgccacc
     8461 ttggattgca gctcccatag ccactacttc gtccgggttt acacctttgt taggttcttt
     8521 accgatttct tttttgacag cttcttgtac cgctggaata cgagttgatc caccaactaa
     8581 gataacttca tcgatatctg agtttgttag gccagcgtct ttcattgctt ggcgtgtagg
     8641 ttccattgtt cttctaatta atgaatctga taattcctca aatttagaac gagttaagtt
     8701 tacttctaag tgtaatggac cgttttcacc agctgagata aatggtaatg agatttgagt
     8761 ttgtgataca cctgataagt cttttttagc tttttcagca gcatctttca aacgttgtaa
     8821 tgccatttta tcttgagata agtctacgcc attttctttt ttgaattctg caactaggta
     8881 gtcaataatt acttggtcaa aatcatcacc gccaagtttg ttgtcaccgg ctgttgatag
     8941 tacttcgaat acaccgtcac ctaattctag gatagatacg tcaaatgtac cgccacctag
     9001 gtcaaaaaca agaacttttt catctttatc agttttgtct aaaccatatg ctaatgctgc
     9061 agctgttggt tcattaatga tacgctcaac ttctaaacca gcaattttac cagcatcttt
     9121 agttgcttga cgttcagcat cgttaaagta tgcaggtact gtaattactg ctttgtcaac
     9181 tttctcacct aaatagcttt cagctgtatt ctttaaattt tgtaaaatca tagctgagat
     9241 ttcttgtggt gtgtatgatt taccttcaat atctacttta taatcagtac ccatatgacg
     9301 tttaatagat tgaacagtat ttgggtttgt aatagcttga cgtttagcta cttcaccaac
     9361 ttgagtctct ccatttttga aagctacaac agatggtgtt gtacgtgaac cttcagggtt
     9421 ttgaattact tttggctcat cgccttctaa tactgttaca catgaatttg ttgtacctaa
     9481 gtctatacca ataattttac tcataataaa attcctccat ttaatcatta aattaatttg
     9541 attttaaaca atgtcgtttc gccaaattta tgttattggt ttactttgac cattgatggt
     9601 cttaatactc tatctttcag cttgtatcct ttttgtagtt cttgagtgat ttcgccagat
     9661 ttaaaatcag ggttatcatc ttgaactaca gcttggtgaa tatttggatc aaatgcttca
     9721 ccttcagttt taataacttc aagaccatta tcttttagtg cgttaatcaa actttcatgc
     9781 accatttgta cacctttttg aagagattta aaagtctcat catcaccttc aatttgaagt
     9841 gcacgttcta tattgtctat tgctggtaaa atatctgtta acacacgttg tgcttgatat
     9901 gttttattta tttcattttc tttttgaatt ctacgcttat aattttcaaa ctcagcgtag
     9961 agccttaaat atttctcttc gttttcatct gctaattgtt gaagttcatt aattttttga
    10021 tcttttggat ctatttcttc aataacattc tcgtcagacg tttcttctat tgcttcatct
    10081 tgtaaatgac ctttactttc ttcagcttgt tcaactgaat catcaatatt ttgtttgacg
    10141 tttgtttctt caactgttga ttcagtgttt ttttcaactg attcgtcttt atttgtcatt
    10201 ttctgtcctc caatactttc taatccatca ttaccaaatt ctatttaata attgaatgac
    10261 attttgataa tgcatagctg taggtccaat cacagcaatt tgacctttta acgtttcatc
    10321 aaaatgatat tgacttgtta caattgaaat atcactcaag ctgtcatcaa tttcatcacc
    10381 aatttttaca ttaatatttg gtgaagatat atcttgtaat aattctgcaa ttctatttga
    10441 ttctatatat tgtagaatgg gctgaattga agatacatta ctttcattca atgcatcaat
    10501 aagtttaacc tttccaccca tataaatgct attactttga ttagaaatat gattattcat
    10561 cgtatttaac aatttattga taaatatttc ttcctgctct gattgaacaa aagagacaat
    10621 atcatcttgt aaattctgat taaactcagt tagtttgtta gtaacaaaat ttgatattgt
    10681 atttagtttg tcattactaa acggcatgtc tgaagcaaga tgtacatgct caacatgacc
    10741 tgatgaaaat acgataacca ttataactaa attaggatta gcacgaatc
//
LOCUS       DAPIDM010000085        10561 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000085 DAPIDM010000000
VERSION     DAPIDM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.085"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            69..2348
                     /gene="secDF"
                     /locus_tag="RJE42_001833"
     CDS             69..2348
                     /gene="secDF"
                     /locus_tag="RJE42_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDV5877247.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQYWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            2551..4824
                     /gene="recJ"
                     /locus_tag="RJE42_001834"
     CDS             2551..4824
                     /gene="recJ"
                     /locus_tag="RJE42_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV5877248.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDVNHDALQLSDMTKAIDRIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSILPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPMSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCEH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            4846..5364
                     /locus_tag="RJE42_001835"
     CDS             4846..5364
                     /locus_tag="RJE42_001835"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV5877249.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            5792..7981
                     /locus_tag="RJE42_001836"
     CDS             5792..7981
                     /locus_tag="RJE42_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV5877250.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYCSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            7993..8445
                     /locus_tag="RJE42_001837"
     CDS             7993..8445
                     /locus_tag="RJE42_001837"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV5877251.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQDSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVNINNDGPVTIIYESQDGKIQ"
     gene            8442..9317
                     /locus_tag="RJE42_001838"
     CDS             8442..9317
                     /locus_tag="RJE42_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDV5877252.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            9778..>10561
                     /locus_tag="RJE42_001839"
     CDS             9778..>10561
                     /locus_tag="RJE42_001839"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV5877253.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDY"
BASE COUNT         3901 a         1414 c         2013 g         3233 t
ORIGIN      
        1 gatttaactt gcgttataaa atagtattca agtattctaa atacatacgt aaataatgag
       61 gtgttcatgt gaagaaaagt agtagaataa ttgcgttctt gcttttagtc gtattgttgt
      121 ttgctggaat ggctgcaact tataaaagtg ttataaagaa tgtcaattta ggacttgatt
      181 tgcaaggtgg atttgaggta ctttatcaag tcgacccttt aaataaaggc gacaaaatag
      241 ataaaaaagc cttacaatca acagcgcaaa cattagaaaa tcgtgttaac gttttaggtg
      301 tttccgaacc taaaattcaa gttgaagaac ctaatagaat tagagttcaa cttgctggtg
      361 taacagacca gaatgaagca cgtaaaattt tatcttctca agctaattta acaattcgcg
      421 atgcagaaga taaagtaaaa ttaagcggtt ctgatattaa acagggatct gccaaacaag
      481 aatttaaaca ggaaacaaac cagccgacag ttacttttaa agtaaaagac aaaaacaaat
      541 ttaaaaaagt aacggaagaa atctctaaga aacgtgataa tgtcatggtt gtttggttag
      601 atttcaaaaa aggtgacagc tataaaaaag aagcacaaaa gaaaaaccca aaatttattt
      661 ctgcagcaag tgttgatcaa ccaatcaatt ctgatagcgt agaaatctct ggtggattta
      721 aaggacaaga aggcgttaaa aaagcaaaac aaatcgccga attgttaaat gcaggttctt
      781 taccagttga cttgaaagaa atttattcaa actcagtagg tgcccaattt ggtcaagatg
      841 cattagataa aactgtgttt gcatcgttta ttggtgtagc attaatttat ttattcatgc
      901 ttggattcta ccgcttacct ggtttagtag cgattattgc attgacaact tatatctatc
      961 taactttagt agcatttaat ttcatttccg gggttctaac tttaccagga ttagcggcgt
     1021 tggtattagg tgtaggtatg gctgtagatg ccaatattat catgtatgag cgtattaagg
     1081 atgaacttcg aataggtaga acgataaagc aagccttttc taaagcaaac aaaagttcgt
     1141 tcctaacaat ttttgattct aacttaacaa cagttatcgc cgcagcagta ttattcttct
     1201 tcggtgaaag ttcagttaaa ggtttcgcga caatgttatt attaggtatt ctaatgatct
     1261 ttgttacagc cgtgttctta tcaagattct tattatcatt acttgtttca tcaaatatat
     1321 tcaaaaatca atattggtta tttggtgtta aaaagaataa acgacatgat attaatgaag
     1381 gtgtagatgt tcatgacctt aaaacttcat tcgagaaatg gaattttgtt aaattagcta
     1441 agccattaat tggagtaagt attttaattg tagttgtcgg tttagttatt ctttatatct
     1501 tcaagttaaa cctaggaatt gatttctcaa gtggtacacg tgtagatttc caatctaaac
     1561 aagcaattac tcaacaaaag gttgagcaag ttgtaaaaga tagtggactt aaagcggatc
     1621 aaattcaaat caatggtaaa gataataaag tagcaactgt tcaatttaaa gacgatttaa
     1681 cgcgtgctca agataataaa ttgagtgata atattaaatc gaaattcggt gacacacctc
     1741 aaattaacac tgtatcacca attattggac aggaattagc aaaaaatgca atgcttgcat
     1801 taatctatgc atcgataggt atcatcatct atgtatcatt acgatttgaa tggcgcatgg
     1861 gtctttcatc tgtattggca ttattacatg atgtattcat tatagtagcg attttcagtt
     1921 tatttagaat tgaagtagat ttaacattta tcgccgctgt attaacaatt gtcggttatt
     1981 caattaatga tacaatcgta acgtttgacc gtgtacgtga aaacttacaa aaggttaaag
     2041 tgattacgac aacagaacaa attgatgata tcgttaatag atcaattaga cagacgatga
     2101 cacgttcaat taatacagta ttaacagtta ttgtagtagt agttgctata ctattcttcg
     2161 gtgctcctac gatattcaac tttactttag cattatttat cggattgatt tctggtgtat
     2221 tctcttcaat cttcattgcc gttccgctat ggggaataat gaaaaaacgt cagttgaaaa
     2281 aatcgccgaa acacaaatta gttgtatata aagaaaagaa atcgaacgat gaaaagattt
     2341 tagtttaaaa tgaattaagc ggtatgtgaa acattaaaga gtttacatac cgctttttac
     2401 tttatttaaa tttaacaaaa tatatataaa taagttcccg cattcatttt taaaaaatgg
     2461 tataattaaa gtcttgatta tttagataag tgctttttat ttaaagtaat tttagatata
     2521 atgtcttttg gaaatgaggc gtaaatatat atgattaaac cgaagtataa gtggaaatta
     2581 actaaaccag ctgaatacat aagtgatgaa ttaacatcaa aattaaagtt aacgccaatc
     2641 gtaaaaaaaa tattagaaag taaatcgatt attgatgaac aagcaattga atccatcatt
     2701 tcagacacag atgtcaatca tgatgcattg caattgagcg atatgactaa ggctattgat
     2761 agaattaaaa gagcgatagc gaatgatgag aaaatcctag tttatggtga ttacgatgcc
     2821 gacggtgtta cctcaactac aattttagtg acaacattac aacttttagg tgcacaagta
     2881 ggatggcata ttcctaaccg ctttaccgaa ggatatggac caaacgaact agcatttaga
     2941 aatgctcatg atgaaggcat aacattaatc ataactgttg ataatggcat acaaggtcat
     3001 aatgaaatta aaatggttca agatttagga gttgatgtca ttgtaactga tcatcatgaa
     3061 attggtagta tattaccaga agcatatgca atcgtacatc caatgcatcc atcgtttaat
     3121 tatccgttcc aacaattgtg tggtgcaggt gttgcgtaca aattagcaca agcattgatt
     3181 gaaaatgtac ctgattattt taaagcatta gttgcgatag gtaccattgc ggacttagta
     3241 tccttaactg atgaaaatcg atcattagtg aaacaaggat taaaagtctt aaatgatcaa
     3301 tgtccgacct ctgttaaagc attgctaaaa gaagcgggat acaatgacaa tattgatgaa
     3361 gaaacaattg gttttattat tggacctaga cttaatgctg taggtcgttt agatgacgca
     3421 tcattagctt gcgaattgtt gatgactgac gacgaagaag aagctgcatt tttagcagaa
     3481 caagttgaac attttaaccg tgaaagaaaa gatattgtag caactatcac agaagaagca
     3541 atggctatgg cagaaacgaa agttaaaaat ggagatttat ttttactttt agcaaaagaa
     3601 aattggcatg aaggtgtctt aggtattgtt gcttcgaaaa ttgtcgaaac atttgctttg
     3661 ccaacattaa ttttaaatat tgatagggag caaaatcatg caaaaggttc cgccagatca
     3721 attgatcaag tgtcgatgtt tgaaatacta tcagcacatc aagagttaat cgctaaattt
     3781 ggcggccatc atatggctgc aggtatgacg atggatattg aaaatataga atcgttagct
     3841 gaaggtttaa ataaatggat gaaagaatta tctaaaacaa cttcgttaga tcccgtaaag
     3901 ccagtagatg tattattaac agaaaatgat ataacgatta aaaatattcg tgatatgaat
     3961 cgtttaagac ccttcggaac agatttttct agacctattt ttgaaatgga tgatttatca
     4021 gtttcttcag ttaaggcgat aggtcaacaa aaaaatcatc taaaattgac acttggtgaa
     4081 agtaatatcg ccgcgctatt ttggcaaaat ggacacttag aacctgagtt acaagatgaa
     4141 cagccaatta atatattagg atctgttcaa atcaacgaat ggaatggtaa tcaatcaccg
     4201 caaataatta ttcaagatat tgcgatgaat gaacagcaaa tattagatta tagaagtaag
     4261 agaaaaagtt taccttttac agaaaatgat gaaaatattg tcgtgcttat tcatcctaaa
     4321 agtgataaag taaatgcgaa tgaatattat tatggtgaag aaattaaaca acaaactgat
     4381 aaagtagtat taagagattt accaatgtca atggaagact tgtctaattc cttgcaacaa
     4441 ctgcaatttt ctcaacttta tatagttttg caacataatc attcgattta cttcgatggt
     4501 atacctaata tggatatttt taaaaagtgt tataaagcat taataactaa acaagaaaca
     4561 aatatccaga aagagggtat gttattgtgt gaacatttaa gtgtgaaacc agatacactt
     4621 aaattcatgt tgaaagtttt cttagactta aaatttgtaa cacaagaaga tggtttaatt
     4681 cgaatcaatc aacaacctga taaaagatcg attgattcca gcaaagtata tcaattaaga
     4741 caacaacgta tggatgttga aaagcaatta ttatatcaag atttttcaga aataaaaaat
     4801 tggataaagt cacaattgtc gtgagcaatt taggaggaaa tattaatgga tttaaagcaa
     4861 tacgtatcag aagttcaaga ttggccgaaa ccaggtgtta gtttcaagga tattactaca
     4921 attatggata atggtgaagc atatggctat gcaacagata aaattgtaga atacgcaaaa
     4981 gacagagatg ttgatatcgt tgtaggacct gaagcgcgtg gctttatcat tggctgtcct
     5041 gtagcttatt caatggggat tggctttgca cctgttagaa aagaagggaa attacctcgt
     5101 gaagtcattc gttatgagta tgacctagaa tatggtacaa atgttttaac aatgcacaaa
     5161 gatgcaatta aaccaggtca acgtgtgtta attacagatg atttattagc tactggtggt
     5221 acgattgaag cagcaataaa attagtcgaa aaattaggcg gtatcgtagt aggtattgca
     5281 tttataattg aattgaaata tttaaatggt attgaaaaaa ttaaagatta cgatgttatg
     5341 agtttaatct catatgacga ataataaata atataatttt atcaaatgaa atccttcatc
     5401 aaatgtataa gaaccaatga cttaattaaa aaagttgttt aagttttctt aacatgagat
     5461 gttaggattt tttatttact gaaaatgtta gatgattgag cattatacct taataacatc
     5521 gtttatttat ttcataaatt gtagtatcat agaactaata tttaaaaaat gaaacagtag
     5581 atttaggtcg aatttttgta aaagttttaa aagtaggaat agtatacaaa ttaaactcgc
     5641 tcaagtaaaa ttaatattac gattaatgac gacaggataa atatttatcg tcgacggacg
     5701 tatgattggt gtgggacaaa tactattcaa caagagtacc taaatcattg tttaaggcga
     5761 agtaataaat atgaatgggg tgtatcatat aatgaacaac gaatatccat atagtgcaga
     5821 cgaagttctt cacaaagcaa aatcatattt gtcagcagat gaatatgagt atgttttaaa
     5881 aagctatcat attgcttatg aagcacataa aggtcagttc cgaaaaaacg gattaccata
     5941 cattatgcat cctatacaag ttgcaggtat tttaacagaa atgcgattag acggaccgac
     6001 gattgtcgca ggttttttgc atgatgtaat tgaagataca ccgtatacat ttgaagatgt
     6061 aaaagaaatg ttcaatgaag aagttgctcg aattgttgat ggtgtgacaa agcttaaaaa
     6121 agtaaaatac tgctcaaaag aagaacaaca agctgaaaat catcgcaagt tatttattgc
     6181 gattgccaaa gatgtacgcg taattttggt gaaattagca gacagattac ataatatgcg
     6241 taccttgaaa gccatgccgc gtgaaaagca aattagaatt tctcgagaaa cattagaaat
     6301 ttatgcacct ttagcacatc gtcttggtat taatacaatc aaatgggaac tagaagatac
     6361 ggctcttcgt tatattgata atgtacaata ttttagaata gtcaatttaa tgaagaagaa
     6421 acgtagtgaa cgggaagcgt atatcgaaac ggctattgat agaatacgta ccgaaatgga
     6481 ccgaatgaat atcgaaggcg atataaatgg tagacctaaa catatttaca gtatttatcg
     6541 gaaaatgatg aagcagaaaa agcaatttga tcaaattttt gatttgttgg cgatacgtgt
     6601 tattgtcaat tctattaatg attgttatgc gatacttggg ttggtgcata cgttatggaa
     6661 accgatgcca ggacgtttta aagattatat tgcaatgcct aaacaaaatt tgtatcagtc
     6721 attgcatact acagtagtag gtccaaatgg agacccgctc gaaatccaaa tacgaacgtt
     6781 tgatatgcac gaaattgctg aacatggtgt tgcagcacac tgggcttaca aagaaggtaa
     6841 aaaagtaagt gaaaaagatc aaacttatca aaataagtta aattggttaa aagagttagc
     6901 tgaagcggat catacttcgt ctgacgctca agaatttatg gaaaccttaa aatatgactt
     6961 acagagtgac aaagtatatg catttacccc agcgagtgat gttattgagt tgccatatgg
     7021 tgctgtgccg attgattttg cttatgcgat tcacagtgaa gtaggtaata agatgattgg
     7081 tgccaaggtg aatggcaaaa ttgtaccaat tgactatatt ttacaaacag gcgatattgt
     7141 tgaaatacgt actagtaaac attcatatgg accaagtcgt gattggttga aaattgttaa
     7201 atcgtctagt gccaaaggta aaattaaaag tttcttcaaa aaacaagatc gttcatctaa
     7261 cattgaaaaa ggacgaatga tggttgaagc tgaaataaaa gagcaaggat ttagagtcga
     7321 agatattttg acagagaaaa atattcaggt tgttaatgaa aaatataact ttgcaaatga
     7381 agatgattta ttcgcagctg taggatttgg cggcgtgaca tccttacaga ttgttaataa
     7441 attaactgaa agacaacgta ttttagataa acaacgtgct ttaaatgaag cacaagaagt
     7501 tacgaaatca ttgcctatta aagacaacat cattactgat agtggtgtct atgtagaagg
     7561 tttagaaaat gtgcttatca agttgtcaaa atgttgtaat cctataccag gtgatgatat
     7621 tgtaggttat atcaccaaag gtcacggtat taaagtacat cgcactgatt gcccaaatat
     7681 taagaacgaa actgaacgac taattaatgt tgaatgggta aaatcaaaag acgcaactca
     7741 aaaatatcag gttgatttag aggtaactgc gtatgatcga aatggcttat tgaatgaagt
     7801 actacaagct gttagctcga cagccggtaa tttaattaaa gtttcaggac gatcagatat
     7861 tgataaaaat gcaataataa atattagtgt catggtgaaa aacgtgaatg atgtttatcg
     7921 tgtggtagaa aagatcaaac aacttggtga tgtttataca gtaacaagag tttggaacta
     7981 gaggtgcaaa atatgaaagt agttgtacaa agagttaaag aagcatcggt gacgaatgat
     8041 acattaaata atcaaatcaa aaaaggatat tgtttattag tcggtatcgg tcaggactct
     8101 acagagcaag atgcagatgt aattgcaaag aaaattgcta atgcaagatt atttgaagat
     8161 gacaataata aattaaactt taatatccaa caaatgaatg gtgaaatact atcagtttca
     8221 caatttactc tctatgcaga tgtaaaaaaa ggtaaccgtc caggtttctc aaattctaaa
     8281 aatcctgatc aagcagtaaa aatatatgag tattttaatg atgcgttacg agcgtatggt
     8341 cttactgtga aaacaggtga atttggaaca cacatgaatg ttaacataaa taatgatggt
     8401 ccagtcacta ttatttatga aagtcaggac ggcaaaattc aatgaaaaaa atagaggcat
     8461 ggttatctaa aaagggtctt aaaaataaac gtactctaat agtagtgatt gcctttgtct
     8521 tatttatcat ctttttattt ttattgctga atagcaatag tgaagatagt gggaacatca
     8581 caataactga aaatgctgaa ttacgtacag gtccaaacgc tgcgtatcca gtcatttaca
     8641 aagttgaaaa aggtgaccat tttaaaaaga ttggtaaagt aggtaaatgg attgaagttg
     8701 aagatacatc cagtaatgaa aaaggttgga tagctggatg gcacacaaat ttagatattg
     8761 tcgcggataa tacgaaggag aaaaatcctt tgcaaggtaa aacaatagtg cttgatcctg
     8821 gtcatggagg tagtgaccag ggtgcttcaa gcaatactaa atataaaagt ttagaaaaag
     8881 attatacgtt gaaaacagca aaagaattgc agcgtacttt agaaaaagaa ggcgcaactg
     8941 ttaagatgac aagaacagac gatacatatg tttcactaga aaatcgtgat atcaaaggcg
     9001 atgcttattt gagtatacat aatgatgcat tagaatcatc taatgcaaat ggaatgacag
     9061 tttattggta tcatgataat caaagagctt tagcagatac gttagacgct acgattcaga
     9121 agaaaggtct actttctaat cgcggttcaa gacaagaaaa ttatcaagtg ttaagacaaa
     9181 caaaagttcc tgctgtttta ttagaattag gttatattag taacccaact gatgaaacga
     9241 tgattaaaga tcaattacat agacaaattt tagaacaagc aattgttgat ggccttaaaa
     9301 tttatttttc tgcgtagggc ttgcaaaaat atgtgaaagt agttatcatt gatattgaat
     9361 tttataacta aaaccgttag tattcttgaa atggtaaatg aaataggtag caatctaact
     9421 aagattgtgt aggaatataa tccatagact gaaagattat gctgagtagt ttatatacat
     9481 tgaacacaag aagaggtgct ttatgaaaag taaagccgtt aaacgtacgt taaacgtttt
     9541 gagtgggttt attaaatgca cgcttgtaaa aagtaatgat gattacaatt aggcatgttt
     9601 tttaaaccaa tgagggtggc aacacggata ttcgtccctt gtgcagtttc aggctgtgca
     9661 agggcttttt cttttgcttt taaaattatg atttatcgtg caaagttaag tggtcgtata
     9721 tagttttagt tttaaaaagg taattaaata aaatagtttg ccgagggaga tgtcaaaatg
     9781 attaaaatac ctagagggac gcaggatatt ttacctgaag attcaaagaa atggcgttac
     9841 attgaaaatc aattagatga attaatgaca ttttacaatt ataaagaaat aagaacacca
     9901 atttttgaaa gtacagatct ttttgcaaga ggtgttggtg attcaaccga tgtcgtacaa
     9961 aaagaaatgt atacatttaa agataaaggc gatagaagta ttacattaag acctgaggga
    10021 acagctgcag ttgtgcgttc atatattgaa cataaaatgc aaggtaatcc aaaccaacca
    10081 attaaacttt attacaatgg accgatgttt agatatgaac gtaagcaaaa agggcgctat
    10141 cgtcaattta atcaatttgg tgtagaagct attggtgctg aaaatcctag cgtagatgca
    10201 gaagtattag ctatggttat gcatatttat caatcatttg gattaaaaca tttaaagctt
    10261 gttattaata gtgtagggga tatggcgtct cgaaaagaat ataacgaagc gttagtgaaa
    10321 cactttgaac cagtaattca tgaattttgt tcagattgtc aatcacgttt gcatacaaat
    10381 ccgatgcgaa ttttggattg taaagttgac cgtgataaag aagcgattaa gactgcacct
    10441 agaatcactg atttcttaaa tgaggaatct aaggcatatt atgaacaagt aaaagcttat
    10501 ttagatgatt taggtattcc atatattgaa gatcctaact tagttcgtgg attggattat
    10561 t
//
LOCUS       DAPIDM010000086        10314 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000086 DAPIDM010000000
VERSION     DAPIDM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.086"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            260..2143
                     /locus_tag="RJE42_001840"
     CDS             260..2143
                     /locus_tag="RJE42_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDV5877254.1"
                     /translation="MGMEAYKIEHLNKSYADKIIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKALIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKERLEIQYDIDITRWSELEEIK
                     EQQ"
     gene            2155..3936
                     /gene="recQ"
                     /locus_tag="RJE42_001841"
     CDS             2155..3936
                     /gene="recQ"
                     /locus_tag="RJE42_001841"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDV5877255.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNLLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFVL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIDSVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            4157..5134
                     /locus_tag="RJE42_001842"
     CDS             4157..5134
                     /locus_tag="RJE42_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877256.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLDMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENPIIRDLDIMKPIDEVTSMSAYPIVYD
                     DQQIEVLYQHLSESERVIVMQEDIVGQYVIERKDIFKYLSQKKEVAQHD"
     gene            5127..6641
                     /locus_tag="RJE42_001843"
     CDS             5127..6641
                     /locus_tag="RJE42_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDV5877257.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYDQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKEKGLIK"
     gene            6881..7939
                     /gene="hisC"
                     /locus_tag="RJE42_001844"
     CDS             6881..7939
                     /gene="hisC"
                     /locus_tag="RJE42_001844"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV5877258.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPSHVPVLIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFIPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            8098..8640
                     /locus_tag="RJE42_001845"
     CDS             8098..8640
                     /locus_tag="RJE42_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDV5877259.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(9377..10294)
                     /locus_tag="RJE42_001846"
     CDS             complement(9377..10294)
                     /locus_tag="RJE42_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDV5877260.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKAKDYAKDVDILFILGGDGTVNELINGVMTYDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILSANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISITTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
BASE COUNT         3784 a         1424 c         1889 g         3217 t
ORIGIN      
        1 gaactcggat tgataaaata tcaatccggg tttttattat gttcttttat attatgtttt
       61 aaattatatg tattgataaa aaggtatatt ataaagatca ttagcatagt catcaaagcc
      121 ttatcaagtt ttatttatag ttgaagcaac aatatattaa attatgacac ttaacagtat
      181 cgatttcttt tgatttcaaa aaggtgtata atgtatagaa ctgatttaag tacgtagaga
      241 tttcaacaga agaaggaaga tgggtatgga agcatataaa attgaacatt taaataaatc
      301 ttatgccgat aagattatat tcgataatct agatttatca atttcagaag gtgaaaaaat
      361 aggtttagta ggcataaatg gtacagggaa aagtacgttg ttaaaagtaa ttggtggtat
      421 tgatgatgat tttacagcca atgttatgca tccaaatcaa tatcgaattc gttattcgtc
      481 tcagaaacag gatcttaatg aagatatgac tgtttttgat gcagtattaa gttctgatac
      541 aacaacttta cgcatcatca agcaatatga acaggcagta caagcttatg cagatgatca
      601 aagtgataaa ttgttcaagc gaatgatgga tgcgcaagat gctatggatc aacatgatgc
      661 ttgggactat aacgctgaaa ttaaaacgat cctctcaaaa ctaggtatac atgatactac
      721 taaatacatt aaagaattat ccggcggaca acaaaaacgt gttgtactag ctaaagcatt
      781 aatagaacaa ccagatttat tgttattaga tgaacctacg aaccatttag acttcgaatc
      841 aatcagctgg ttgatcaatt atgtgaagca atatcctcat acagttttat tcgtaaccca
      901 tgatcgatat tttttaaatg aagtttccac tagaattatt gaactaaaca gaggtaagtt
      961 agcgtcatat cctggtaact atgaatctta tattgaaatg cgcgctgaaa gagaagtaac
     1021 acttcaaaag caacaacaaa agcaacgagc tttatataag gaagaacttg cttggatgag
     1081 ggctggagct aaggcccgta ctacaaagca acaagctaga attaatcgat ttaatgacct
     1141 agaaaatgaa gttaatcagc aatataaaga cgataaaggt gaattgaatc ttgcttattc
     1201 aagattaggt aagcaagtgt tcgaattaga agacttatca aaggctatta acgataaagt
     1261 attatttgaa catctgacgg aaattattca aaaaggtgag cgtattggtg ttgttgggcc
     1321 aaatggagct ggtaaaacaa cactcttaaa tattttgagt ggagaagacc aacaattcga
     1381 aggtaaattg aagactgggc agacggttaa agtagcttat tttaagcaaa cagatgagac
     1441 cctggataga gatattcgta tgattgatta tttaagagaa gaaagtgaga tcgcaaaaga
     1501 aaaagatgga acctcggtat ctattacaca acttcttgaa cgatttttat ttccaagtgc
     1561 aactcatggt aaaaaagttt ataaattatc tggtggagag caaaagcgtt tgtatttatt
     1621 acgtctactc gtacaccagc caaatgttct gttgttagat gaaccgacaa atgatttaga
     1681 tactgagact ttaactatac ttgaagatta tattcatact tttggtggta cagtgattac
     1741 cgtaagccat gatcgttact tcttaaataa agttgcacag tcatattggt ttattcatga
     1801 tggtcagatg gaaaagatta tcggaacttt tgaagattat gaaagttata aaaaatcatt
     1861 agataaaaat aaatccacat tgaagcaaca atctaagtct tctacaactg tacgtaagaa
     1921 aaatgggtta tcatataaag aaaaattaga atatgaacaa ttgatgaaac gtatagaaca
     1981 agcggaagta agaatggaag aaattgatgt gctcatgatt gaggcaagtg cagattatgg
     2041 gaaaattaaa gaattaaacg aagaaaaaga acgacttgaa attcaatatg atatagacat
     2101 cacaagatgg agtgagttag aagaaattaa agaacaacaa taaggggtca atttatgatg
     2161 caacaaacat tatcgcatta ctttgggtat gaaacgtttc gaccaggaca agaagagatt
     2221 attagcaaag tattagacca tcgtaatgtg cttggtgtct taccaactgg tggaggtaag
     2281 tctatatgct atcaagtacc aggtttattg ttaggtggta caacaattgt aataagtcca
     2341 ctaatatcat taatgaaaga tcaagtggat caattaaaag cgatgggaat tcaagctgct
     2401 tttttaaata gtagtttgac tcaaaaagag caacaacgta ttgaaaaagc attatcaaat
     2461 ggagaaattc aatttttata tgttgcacca gaacgatttg aaaatcgata ttttttaaat
     2521 ttgcttcaac gtatcaagat tcatttagtg gcgtttgatg aagcacattg tatttcaaaa
     2581 tggggtcatg atttcagacc gagttaccaa aacgtcattt ctaaagtatt tacgttacct
     2641 caagatttta caataatagc gttgacagca actgcaacag ttgaagtaca gcaagatatt
     2701 agagaaaagt taaatatcgc tcaaactgat caaattaaaa cgagtactaa gcgtagaaac
     2761 ttaattttta aagtaaatcc tacttatcaa cggcaaaaat ttgtattaga ttatattaaa
     2821 acacacgatg aagatgcagg tattatttat tgttctacac gtaagcaagt tgaagagctt
     2881 caagaagcct tagaaagcca gaaaattgat agtgttatat atcatgcagg tttgagcaat
     2941 aaagaaagag aagaagcgca gaatgacttc ttatttgatc gtgttaaagt agtcgttgct
     3001 acaaatgctt ttggtatggg tattgataaa tctaatgtac gttttgttat tcattataat
     3061 atgcctggag atttagaatc ttattatcaa gaagcgggac gtgcaggtcg tgacggattg
     3121 aaaagtgaat gtattttgtt atttagcgaa cgcgatatca atttacacga gtattttata
     3181 acagtctctc aagctgatga tgactataaa gataaaatgg gcgaaaagtt aactaaaatg
     3241 attcaatata caaaaacaaa aaaatgttta gaagcaacaa ttgtccatta ttttgaaccg
     3301 aatgaaaaat tagaagaatg tgaacaatgt agtaattgtg ttcaacaaga taaatcatat
     3361 aatatgacac aagaagctaa gatgattatt agttgcatcg ctcgtatgaa acaacaagag
     3421 agttatagtg ttatcattca agtattaaga ggagagtcaa cagattatat taagtataaa
     3481 ggttatgatc aaatttcaac ccatggttta atgaaaggtt acactacatc agaattaagt
     3541 cacttaatag atgaattaag attcaaaggg ttcttaaatg aaaatgatga aatattaatg
     3601 tgtgatactt caattaaaaa attactcagt aatgaagtag aagtattcac aacaccattt
     3661 aagcaaaaag cgactgaaaa agtatttata aatacggttg aaggggttga ccgagtatta
     3721 ttcagtcagt tggtagaagt tcgtaaaaag ttaagtgaca aattaacgat agcacctgta
     3781 agtatatttt ctgattacac gttggaggaa tttgctaaac gtaagcctgc ttcgaaacaa
     3841 gatatgatta atattgatgg cgtaggtagt tacaaattaa aacattattg tccagcattt
     3901 ttagaaacga ttcaaaatta taaagccaaa gtatagtgaa ggcacctagt aaacattcct
     3961 tgttttactg ggtgtttttt gaatttaaag taaaataaca ctatttctta actggagatt
     4021 acctattatt cattattttg ttaaaaaagt tcaaggataa atcatttaaa tttgaaaatt
     4081 gtaataaacg tggttaaata gggaatacat acatataatc tcggttttat gctatgagaa
     4141 aaaaagaggt ggcaaagtga ttaagtttaa aaatgtaact aagcgttatg gcaaacatgt
     4201 tgctgtcgat aacattagtt tcaatattaa tgagggtgaa ttttttgtgc taattggacc
     4261 ttcaggttgt ggaaaaacta cgacattaaa aatgattaat cgactcattc acttaagtga
     4321 aggttatatt tattttaaag ataaaccaat aagtgattat ccagtatacg aaatgcgttg
     4381 ggatattgga tacgtattgc agcagattgc attattccca catatgacaa tcaaagaaaa
     4441 tattgcacaa gtgccacaaa tgaaaaagtg gaaagaaaaa gatatagata aaagagtaga
     4501 tgaattactt gatatggttg gattagaacc tgaaaaatat aaaaacagaa aacctgatga
     4561 attgtcaggg gggcaacgac aacgtgtagg agttatacgt gcgttagcag ctgatccacc
     4621 agttatttta atggatgaac cgtttagtgc attagaccca atcagccgag aaaaacttca
     4681 agatgattta attgaattac aaactaaaat taagaagaca atcatatttg ttacacatga
     4741 tattcaagag gcgatgaaac ttggtgataa gatttgtctt ttgaatgaag gtcatattga
     4801 acaaattgac acaccagaag gatttaaaaa taatcctcaa agtgaatttg ttaaacaatt
     4861 tatgggtagt catttagaag atgatgcgcc atgtgttgaa gagaacccaa ttatccgtga
     4921 cttggatatt atgaaaccaa tcgatgaggt tacatctatg agcgcttatc caattgttta
     4981 tgacgatcaa caaattgaag tattgtatca acatttatca gagagcgagc gtgtcattgt
     5041 catgcaagaa gatatcgtag gtcaatatgt tattgaaagg aaagatatct tcaaatattt
     5101 gtcccagaaa aaggaggtag ctcaacatga ctaacttttt cgacatattg agtgaacgta
     5161 aggggcaact attttcgaca atgatagaac atattcaaat atcatttatc gcattattga
     5221 ttgcaactgc tattgcggta ccattaggta ttttattgac gaagactaaa acgatatctg
     5281 aaatcgtaat gaatattgcg gcaattcttc aaactatacc atcgttggca ttattaggtt
     5341 taatgattcc tttatttggt atcggtcgtg tgccagcaat tattgcactt gtagtgtatg
     5401 cattgttacc aattttaagg aatacgtata ctggaattaa agaagttgat ccatcgctca
     5461 ttgaagcggc taaaggtata ggtatgaagc catttagacg tttaactaaa gttgaacttc
     5521 caatagcaat gcctgttata atggctggtg taagaacggc tatggtatta attataggta
     5581 cagcaacact agcagcatta attggtgcag gcggactagg tgatttaatt ttattaggta
     5641 tagaccgtaa caatgcatca ttgatattat taggtgcaat tccagcagcc ttattggcaa
     5701 ttatatttga tttaatttta agatttatgg ctaaattatc ttataaaaag ttattgatga
     5761 cgttaggtgt tatagtgatg attattatac tggccatcgc tattcctatg tttgcacaaa
     5821 aaggtgataa aattacgtta gctggaaagc ttggctccga gccttcgatt attacaaata
     5881 tgtataaaat tttaatagaa gaagagacca aaaatactgt agaagtgaaa gatggtatgg
     5941 gcaaaacagc atttttattt aatgctttaa aatctgacga tatagatggg tatttagagt
     6001 ttactggaac agttttaggt gagttaacaa aagaaccatt gaagtcaaaa gaagagaaaa
     6061 aagtatatga tcaagctaag caaagtcttg aaaagaaata tcaaatgact atgttaaaac
     6121 caatgaaata taacaatacg tatgctttag ctgtaaaacg tgattttgct aaacaacata
     6181 atatacgtac aattggtgat ttaaataagg ttaaagatca acttaaacca ggatttacat
     6241 tggaatttaa tgatcgtcca gatggttaca aagctgttca aaaggcttat aatttaaatt
     6301 tagataacat acgtacaatg gaacctaagt tgagatatca agcgatcaat aaaggtaata
     6361 ttaatttaat agatgcatat tcaactgacg ctgaattaaa acaatatgat atggttgtgt
     6421 taaaagatga taagcacgta tttccaccat atcaaggagc accattattt aaagaaagct
     6481 ttttaaagaa acatccagaa attaagaaac cgttaaacaa actagaaaac aaaatatctg
     6541 atgaagatat gcaaatgatg aactataaag taacagttaa aaatgaagac ccatatacag
     6601 ttgcgaaaga ttatttaaaa gaaaaagggt taatcaaata acgaccaacg ccacataaga
     6661 tgaggggcac accattatat cttatgtggc gttgctatat ttacatctat gtttatgttc
     6721 aatttaaaca tgcaataatg attaaaaaat atgacatgtt aaacataatg aaagctagta
     6781 tgatgtgaaa atagtagcat tgcattttag aaacatagag cgatataatg aatataagtt
     6841 ttttaaaatt tcagttaatt ctaaggaggt tgtttttatt atgaaagaac aacttaatca
     6901 actatcagca tatcagcctg gtttatctcc aagggcattg aaagaaaagt atggcattga
     6961 aggagattta tataaacttg catcaaatga aaatttgtat ggaccatcgc ctaaagttaa
     7021 agaagcgata tcagcacact tagatgagtt atattattat cctgaaacag gatcaccgac
     7081 attaaaagcg gcgattagta aacatttaaa tgtagatcaa tcacgcattt tatttggtgc
     7141 gggattagat gaagttatat taatgatttc tagagcagta ttaacgccag gggatactat
     7201 tgttacaagt gaagcgacat tcggtcaata ttatcacaat gcgattgttg aatcagctaa
     7261 tgtgatacaa gtacctttaa aagatggtgg cttcgattta gaaggtattt taaaagaagt
     7321 taatgaagat acgtcattgg tatggttatg taatccaaat aatcctacag gtacgtattt
     7381 taatcatgag agcttagatt cgtttttatc tcaagtacca tcacatgttc cggtactcat
     7441 agatgaagct tattttgaat ttgtgacagc agaggactac ccggatacac ttgctttgca
     7501 acaaaaatat gacaatgcat tcttattacg tacattttca aaggcgtatg gattagcggg
     7561 tttacgtgta ggatatgtgg tagcaagcga acatgcgatt gaaaaatgga acatcattag
     7621 acccccattt aatgtgacac gtatatctga atacgcagca gttgcagcac ttgaagatca
     7681 acaatattta aaagaggtaa cacataaaaa tagtgttgaa cgcgaaagat tttatcaatt
     7741 acctcaaagt gagcatttca taccaagtca aacgaatttt atatttgtaa aaacaaagcg
     7801 ggtaaatgaa ctttatgaag cacttttaaa tgtagggtgt attacgcgac catttccaac
     7861 tggtgttaga attacaattg gttttaaaga acaaaatgat aaaatgttag aagttttatc
     7921 aaactttaaa tacgaatagt aagtgggcac cacatggtat gaatatgtga caaaatgcag
     7981 tgtgaatgat gaggtgagag cattcagtgt aaaaagcaac aatagatgat attgttttgt
     8041 atcaattgct tttttgctat actgaattaa tactgatatt ttgaggagaa gattaaaatg
     8101 acccgtaaat caatcgcgat tgatatggat gaagtattgg cagatacatt aggagaaatc
     8161 attgatgctg tcaattttag agcggattta ggtattaaaa tggaagcttt gaatggtcaa
     8221 aaacttaagc atgttattcc tgaacatgat ggattaatta cagaagtatt aagagaacca
     8281 ggcttcttca gacaccttaa agtgatgccg tatgcacaag aagttgtaaa aaaattaact
     8341 gaacattatg atgtatatat tgctacagca gcaatggatg taccaacatc attcagtgat
     8401 aaatatgaat ggttactaga gttctttcca ttcttagatc ctcagcattt tgttttttgt
     8461 ggtagaaaaa acatcgttaa agctgattat ttaatagatg acaatcctag acaacttgaa
     8521 atttttactg gtacaccgat tatgtttaca gcagtgcata atattaatga tgatcgattt
     8581 gaacgcgtaa atagctggaa agatgtagaa cagtattttt tagataatat tgagaaataa
     8641 aaatatatca cttgaagcat ttcatgtagc aaataaaatg catatactgt ggccccaaca
     8701 cagagaattt tgaaaagaaa ttctacaaac aatgcgagtt ggggtggccc caacataaag
     8761 aaatattttt tctttagaaa ttagtatttc ttatgggagt gggacagaaa tcaatgttct
     8821 atgctctatg aagttatatt ggcagtagtt gactgaacga aaatgcgctt gtaacaagct
     8881 tttttcaatt ctagtcaggg gccccaacaa agagaatttc gaaaagaaat tctacaaaca
     8941 atgcaagttg gggtgggacg acgaaataaa ttttgcgaaa atatcatttc tgtcccactc
     9001 cctcctattt ttaagtacac attagctgtg actaatgata aagaatcgct acataatcaa
     9061 tcattagtcg ttctttatca tttccgtccc gctctcaata aatgttagtc tatctcatta
     9121 ttataaatcg gatgaatgtg ttaatctttg gcagattaca cgtcatccga ttttttatag
     9181 aatttgaaaa agacgcataa accactatga tttaaaatac aacatcaatc attttagtgg
     9241 catgcgccaa aattatattt ctgtttttga aacaggataa tagcttaaag ctcataacaa
     9301 cgaatattag gtacgttgaa tcttattatt acactttaaa cctaatataa tatcgcgtta
     9361 atatcattct ggatgcttac aaatcattga cagtaagcaa ctgaatggca tttggtataa
     9421 cctcaatatc aataggtgtt tctaatgaaa tttcgccatc aatatcaact ttcattgctg
     9481 gatctgttgt aattgaaatc tttttaccag gtatatgctc aataccttga gtaatttcat
     9541 tccaattcat gctatcacgc tttttaaaaa tatcatttaa aatactgaaa ctttgttcat
     9601 taaaaatgaa agtgttcagt tcaccatctt gaggagacaa atcagtcaat ggtatacgac
     9661 taccaccaat gaatggacca tttgctgata gtatcatggt cgtttcgcca gaatatgtct
     9721 tatcatcaat tgataattga taattaaatt gtgttggatt tagcagtgtt ttgacagttg
     9781 atccaatata actcaattta ccaaatatat cttttgaacc atcttgtacg ttttcagcgt
     9841 tttgaacaat tagacctaag ccaacaaagt tgagtgcata ttgattattt attttaatta
     9901 catcgtatgt accaacttgt gcagaaatca tttgttcact agcttgttta tgattaggtg
     9961 ctatatttag cgtttttgta aaatcattaa aagtaccgcc tggtaaaatg ccaataggga
    10021 gttgaaggtc atacgtcata acaccattta taagttcgtt aaccgtgcca tcaccgccaa
    10081 gaataaataa tatatctaca tcttttgcat aatctttagc tttaatttct tggcaatatt
    10141 taattatgtc accttcgttt tcactcaatt gaatagaaag atgcttacaa attgaactta
    10201 atgccgttgt aacttccccg ataccttgat taatattttt taatccactg tgttcatggt
    10261 aaaagaggac accatgtgta tatttatttt ccatagttta gcctactttc taaa
//
LOCUS       DAPIDM010000087        10307 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000087 DAPIDM010000000
VERSION     DAPIDM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.087"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     misc_feature    <1..109
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            147..647
                     /locus_tag="RJE42_001847"
     CDS             147..647
                     /locus_tag="RJE42_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV5877261.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            690..1058
                     /gene="rplL"
                     /locus_tag="RJE42_001848"
     CDS             690..1058
                     /gene="rplL"
                     /locus_tag="RJE42_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV5877262.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            1233..1841
                     /locus_tag="RJE42_001849"
     CDS             1233..1841
                     /locus_tag="RJE42_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV5877263.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLKHHKIDLITDNGVFSKDKVDYG
                     SDVLVKTFLKTHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            2056..5607
                     /gene="rpoB"
                     /locus_tag="RJE42_001850"
     CDS             2056..5607
                     /gene="rpoB"
                     /locus_tag="RJE42_001850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV5877264.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            5771..9367
                     /gene="rpoC"
                     /locus_tag="RJE42_001851"
     CDS             5771..9367
                     /gene="rpoC"
                     /locus_tag="RJE42_001851"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV5877265.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEVI
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            9504..9758
                     /locus_tag="RJE42_001852"
     CDS             9504..9758
                     /locus_tag="RJE42_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDV5877266.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKYVGINVNATIVALIK"
     gene            9856..10269
                     /gene="rpsL"
                     /locus_tag="RJE42_001853"
     CDS             9856..10269
                     /gene="rpsL"
                     /locus_tag="RJE42_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV5877267.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
BASE COUNT         3610 a         1615 c         2071 g         3011 t
ORIGIN      
        1 ttataactta aaatttatcc taccgaggct aaaattgact tgaacgtgat gatctatgat
       61 ctttcaagca ctttttgccg tgggtagaaa gtgctttttt tattaatttt aaaaaaagca
      121 ccaaaaattt aaatggaggt gtctgaatgt ctgctatcat tgaagctaaa aaacaactag
      181 ttgatgaaat tgctgaggta ctatcaaatt cagtttcaac agtaatcgtt gactaccgtg
      241 gattaacagt agctgaagtt actgacttac gttcacaatt acgtgaagct ggtgttgagt
      301 ataaagtata caaaaacact atggtacgtc gtgcagctga aaaagctggt atcgaaggct
      361 tagatgaatt cttaacaggt cctactgcta ttgcaacttc aagtgaagat gctgtagctg
      421 cagcgaaagt aatttctgga tttgctaaag atcatgaagc attagaaatt aaatcaggcg
      481 ttatggaagg caatgttatt acagcagaag aagttaaaac tgttggttca ttaccttcac
      541 acgatggtct tgtatctatg cttttatcag tattacaagc tcctgtacgc aacttcgctt
      601 atgcggttaa agctattgga gaacaaaaag aagaaaacgc tgaataattt ttagcgtaaa
      661 aaaattaaaa ataatggagg aattataaaa tggctaatca tgaacaaatc attgaagcga
      721 ttaaagaaat gtcagtatta gaattaaacg acttagtaaa agcaattgaa gaagaatttg
      781 gtgtaactgc agctgctcca gtagcagtag caggtgcagc tggtggcgct gatgctgcag
      841 cagaaaaaac tgaatttgac gttgagttaa cttcagctgg ttcatctaaa atcaaagttg
      901 ttaaagctgt taaagaagca actggtttag gattaaaaga tgctaaagaa ttagtagacg
      961 gagctcctaa agtaatcaaa gaagctttac ctaaagaaga agctgaaaaa cttaaagaac
     1021 aattagaaga agttggagct actgtagaat taaaataatt caagtatctt aaacttaata
     1081 atcaaagttt tatagcaagc attgctataa tataatgatt ctttgagaag ttaaaacccc
     1141 gttattttga taacggggtt ttattcattt aaagactgag taaaatgtta taattataat
     1201 gacgagttac aaagtgaaga tgaggtggaa taatgagtca ttattatgat gaagatccaa
     1261 gtgtaattag caatgaacaa cgtattcaat atcaattaaa acatcataaa attgatttaa
     1321 taactgataa cggagtgttt tcgaaagata aagtagatta tggttcagat gttcttgtta
     1381 aaactttttt aaaaacgcat ccacctggtc caagtaagcg aattgccgat gttggttgtg
     1441 gttacggacc aattggtttg atgattgcta aagtatcacc acatcattca attacaatgc
     1501 tagatgttaa tcacagagcg ctagccttag ttgaaaaaaa caaaaaatta aacggtattg
     1561 ataatgtgat cgtaaaggaa agtgatgctt tgtctgctgt ggaagacaaa agttttgatt
     1621 ttattttaac caatccacca ataagagcag ggaaagaaac cgtgcatcgt atattcgagc
     1681 aagcattaca tagattagac tcgaacggtg aactattcgt tgtaattcag aagaagcaag
     1741 gcatgccatc tgcaaagaaa agaatgaatg aactttttgg aaatgtagaa gtggtaaata
     1801 aagataaagg atattacatt ctgagaagta taaaagcttg aaatgaaatg gatattctgt
     1861 tatagttata taatgtaaaa atttatgttc aataagtgtg tacttttacg ttaaatagat
     1921 aagttaatta agaataaata tagaatcgaa aatggtgtca tcattagtgt tgccgttttc
     1981 tttttgtctt tttattaata tgcttatggt atttagctaa aagcggatca cataattttt
     2041 gaggggtgaa tctgtttggc aggtcaagtt gtccaatatg gaagacatcg taaacgtaga
     2101 aactacgcga gaatttcaga agtattagaa ttaccaaact taatagaaat tcaaactaaa
     2161 tcttacgagt ggttcctaag agaaggttta atcgaaatgt ttagagacat ttctccaatt
     2221 gaagatttta ctggtaattt gtcattagag tttgtggatt accgtttagg agaaccaaaa
     2281 tatgatttag aagaatctaa aaaccgtgac gctacttatg ctgcacctct tcgtgtaaaa
     2341 gtgcgtctaa tcattaaaga aacaggagaa gttaaagaac aagaagtctt tatgggtgat
     2401 ttcccattaa tgactgatac aggtacgttc gttatcaatg gtgcagaacg tgtaatcgta
     2461 tctcaattag ttcgttcacc atccgtttat ttcaatgaaa aaatcgacaa aaatggtcgt
     2521 gaaaactatg atgcaacaat tattccaaac cgtggtgcat ggttagaata tgaaacagat
     2581 gctaaagatg ttgtatacgt gcgtattgat agaacacgta aactaccatt aacagtattg
     2641 ttacgtgcat taggtttctc aagtgaccaa gaaattgttg accttttagg tgacaatgaa
     2701 tatttacgta atactttaga gaaagacggc actgaaaaca ctgaacaagc gttattagaa
     2761 atctatgaac gtttacgtcc aggtgaacca ccaactgttg aaaatgctaa aagtctattg
     2821 tattcacgtt tctttgatcc aaaacgctat gacttagcaa gcgtgggtcg ttataaaaca
     2881 aacaaaaaat tacatttgaa acatcgttta ttcaatcaaa aattagctga gccaattgta
     2941 aatactgaaa ctggtgaaat tgtagttgaa gaaggtacag tgcttgatcg tcgtaaaatc
     3001 gacgaaatca tggatgtact tgaatcgaac gcaaacagcg aagtgtttga attgcatggt
     3061 agcgtaatag acgagccagt agaaattcaa tcaattaaag tatatgttcc taacgatgat
     3121 gaaggtcgta cgacaactgt aattggtaat gctttccctg actcagaagt taaatgtatt
     3181 acaccagcag atatcatcgc ttcaatgagt tacttcttta acttattaag tggtattgga
     3241 tatacagatg atattgacca tttaggtaac cgtcgtttac gttctgtagg tgaattacta
     3301 caaaaccaat tccgtatcgg tttatcaaga atggaaagag ttgtacgtga aagaatgtca
     3361 attcaagata ctgagtctat cacacctcaa caattaatta atattcgacc tgttattgca
     3421 tctattaaag aattctttgg tagctctcaa ttatcacaat tcatggacca agcaaatcca
     3481 ttagctgagt taacgcataa acgtcgtcta tcagcattag gacctggtgg tttaacacgt
     3541 gaacgtgctc aaatggaagt acgtgacgtt cactactctc actatggccg tatgtgtcca
     3601 attgaaacac ctgagggacc aaacattgga ttgattaact cattatcaag ttatgcacgt
     3661 gtaaatgaat tcggctttat tgaaacacca tatcgtaaag ttgatttaga tacacatgct
     3721 atcactgatc aaattgacta tttaacagct gacgaagaag atagctatgt tgtagcacaa
     3781 gcaaactcta aattagatga aaatggtcgt ttcatggatg atgaagttgt atgtcgtttc
     3841 cgtggtaaca atacagttat ggctaaagaa aaaatggatt atatggatgt atcgccgaag
     3901 caagttgttt cagcagcgac agcatgtatt ccattcttag aaaatgatga ctcaaaccgt
     3961 gcattgatgg gtgcgaacat gcaacgtcaa gcagtgcctt tgatgaatcc agaagcacca
     4021 tttgttggaa caggtatgga acacgttgca gcacgtgatt ctggtgcagc tattacagct
     4081 aagcacagag gtcgcgttga acatgttgaa tctaatgaaa ttcttgtacg tcgtctagtt
     4141 gaagagaacg gcgttgagca tgaaggtgaa ttagatcgct atccattagc taaatttaaa
     4201 cgttcaaact caggtacatg ttacaaccaa cgtccaatcg ttgcagttgg agatgttgtt
     4261 gagtataacg agattttagc agatggacca tctatggaat taggagaaat ggcattaggt
     4321 agaaacgtag tagttggttt catgacttgg gacggttaca actatgagga tgccgttatc
     4381 atgagtgaaa gacttgtgaa agatgacgtg tatacttcta ttcatattga agagtatgaa
     4441 tcagaagcac gtgatactaa gttaggacct gaagaaatca caagagatat tcctaatgtt
     4501 tctgaaagtg cacttaagaa cttagacgat cgtggtatcg tttatattgg tgcagaagta
     4561 aaagatggag atattttagt tggtaaagta acgcctaaag gtgtaactga gttaactgcc
     4621 gaagaaagat tgttacatgc aatctttggt gaaaaagcac gtgaagttag agatacttca
     4681 ttacgtgtac ctcacggcgc tggcggtatc gttcttgatg taaaagtatt caatcgtgaa
     4741 gaaggcgacg atacattatc acctggtgta aaccaattag tacgtgtata tatcgttcaa
     4801 aaacgtaaaa ttcatgttgg tgataagatg tgtggtcgac atggtaacaa aggtgtcatt
     4861 tctaagattg ttcctgaaga agatatgcct tacttaccag acggacgtcc gatcgatatc
     4921 atgttaaacc ctcttggtgt accatctcgt atgaacatcg gacaagtatt agagctacac
     4981 ttaggtatgg ctgctaaaaa tcttggtatt cacgttgcat caccagtatt tgacggtgca
     5041 aacgatgacg atgtatggtc aacaattgaa gaagctggta tggctcgtga tggtaaaact
     5101 gtactttatg atggacgtac aggtgaacca ttcgataacc gtatttcagt aggtgtaatg
     5161 tacatgttga aacttgcgca catggttgat gataaattac atgcgcgttc aacaggacca
     5221 tattcacttg ttacacaaca accacttggc ggtaaagcgc aattcggtgg acaacgtttc
     5281 ggtgagatgg aggtatgggc acttgaagca tatggtgctg catacacatt acaagaaatc
     5341 ttaacttaca aatccgatga tacagtagga cgtgtgaaaa catacgaggc tattgttaaa
     5401 ggtgaaaaca tctctagacc aagtgttcca gaatcattcc gagtattgat gaaagaatta
     5461 caaagtttag gtttagatgt aaaagttatg gatgagcaag ataatgaaat cgaaatgaca
     5521 gacgttgatg acgatgatgt tgtagaacgc aaagtagatt tacaacaaaa tgatgctcct
     5581 gaaacacaaa aagaagttac tgattaatac gcaatttaca aaacaggcaa aaagatacta
     5641 agctgaattt tatagatgat tcagtttagt actttaagcc attttaaata aatgcaaatc
     5701 aatcaaatag cacagctaat ctaaattgaa ggaggtaggc tccttgattg atgtaaataa
     5761 tttccattat atgaaaatag gattggcttc acctgaaaaa atccgttctt ggtcttttgg
     5821 tgaagttaaa aaacctgaaa caatcaacta ccgtacatta aaacctgaaa aagatggtct
     5881 attctgtgaa agaattttcg gacctacaaa agactgggaa tgtagttgtg gtaaatacaa
     5941 acgtgttcgc tacaaaggca tggtctgtga cagatgtgga gttgaagtaa ctaaatctaa
     6001 agtacgtcgt gaaagaatgg gacacattga acttgctgct ccagtttctc acatttggta
     6061 tttcaaaggt ataccaagtc gtatgggatt attacttgac atgtcaccaa gagcattaga
     6121 agaagttatt tactttgctt cttatgttgt tgtagatcca ggtccaactg gtttagaaaa
     6181 gaaaacttta ttatctgaag ctgaattcag agattattat gataaatacc caggtcaatt
     6241 cgttgcaaaa atgggtgcag aaggtattaa agatttactt gaagagattg atcttgacga
     6301 agaacttaaa ttgttacgcg atgagttgga atcagctact ggtcaaagac ttactcgtgc
     6361 aattaaacgt ttagaagttg ttgaatcatt ccgtaattca ggtaacaaac cttcatggat
     6421 gattttagat gtacttccaa tcatcccacc agaaattcgt ccaatggttc aattagatgg
     6481 tggacgattt gcaacaagtg acttaaacga tttataccgt cgtgtaatta atcgaaacaa
     6541 tcgtttgaaa cgtttattag atttaggtgc acctggtatc atcgttcaaa acgaaaaacg
     6601 tatgttacaa gaagctgttg acgctttaat tgataatggt cgtcgtggtc gtccagttac
     6661 tggcccaggt aaccgtccat taaaatcttt atctcatatg ttaaaaggta aacaaggtcg
     6721 tttccgtcaa aacttacttg gtaaacgtgt tgactattca ggacgttcag ttatcgcggt
     6781 aggtccaagc ttgaaaatgt accaatgtgg tttaccaaaa gaaatggcac ttgaactgtt
     6841 taaaccattc gtaatgaaag aattagttca acgtgaaatt gcaactaaca ttaaaaatgc
     6901 gaagagtaaa atcgaacgca tggatgatga agtttgggac gtattggaag aagtaattag
     6961 agaacatcct gtattactta accgtgcacc aacacttcat agacttggta ttcaagcatt
     7021 tgaaccaact ttagttgaag gtcgtgcgat tcgtctacat ccacttgtaa caacagctta
     7081 taacgctgac tttgatggtg accaaatggc ggttcacgtt cctttatcaa aagaggcaca
     7141 agctgaagca agaatgttga tgttagcagc acaaaacatc ttgaacccta aagatggtaa
     7201 accagtagtt acaccatcac aagatatggt acttggtaac tattacctta ctttagaaag
     7261 aaaagatgca gtaaatacag gcgcaatctt taataataca aatgaagtat taaaagcata
     7321 tgcaaatggc tttgtacatt tacacacaag aattggtgta catgcaagtt cattcaacaa
     7381 tccaacattt actgaagaac aaaacaaaaa gattcttgct acgtcagtag gtaaaattat
     7441 attcaatgaa atcattccag attcatttgc ttatattaat gaacctacgc aagaaaactt
     7501 agaaagaaag acaccaaaca gatacttcat cgatcctaca actttaggtg aaggtggatt
     7561 aaaagaatac tttgaaaatg aagaattaat tgaacctttc aacaaaaaat tcttaggtaa
     7621 tattattgca gaagtattca acagatttag catcactgat acatcaatga tgttagaccg
     7681 tatgaaagac ttaggattca aattctcatc taaagctggt attacagtag gtgttgctga
     7741 tatcgtagta ttacctgata agcaacaaat acttgatgag catgaaaaat tagtcgacag
     7801 aattacaaaa caattcaatc gtggtttaat cactgaagaa gaaagatata atgcagttgt
     7861 tgaaatttgg acagatgcaa aagatcaaat tcaaggtgaa ttgatgcaat cacttgataa
     7921 aactaaccca atcttcatga tgagtgattc aggtgcccgt ggtaacgcat ctaactttac
     7981 acagttagca ggtatgcgtg gattgatggc cgcaccatct ggtaagatta tcgaattacc
     8041 aatcacatct tcattccgtg aaggtttaac agtacttgaa tacttcatct caactcacgg
     8101 tgcgcgtaaa ggtcttgccg atacagcact taaaacagct gactcaggat atcttactcg
     8161 tcgtcttgtt gacgtggcac aagatgttat tgttcgtgaa gaagactgtg gtactgatag
     8221 aggtttatta gtttctgata ttaaagaagg tacagaaatg attgaaccat ttatcgaacg
     8281 tattgaaggt cgttattcta aagaaacaat tcgtcatcct gaaactgatg aagttatcat
     8341 tcgtcctgat gaattaatta cacctgaaat tgctaagaaa attacagatg ctggtattga
     8401 acaaatgtat attcgctcag catttacttg taacgcaaga cacggtgttt gtgaaaaatg
     8461 ttacggtaaa aaccttgcta ctggtgaaaa agttgaagtt ggtgaagcag ttggtacaat
     8521 tgcagcccaa tctatcggtg aaccaggtac acagcttaca atgcgtacat tccatacagg
     8581 tggggtagca ggtagcgata tcacacaagg tcttcctcgt attcaagaga ttttcgaagc
     8641 acgtaaccct aaaggtcaag cggtaattac ggaaatcgaa ggtgtcgtag aagatattaa
     8701 attagcaaaa gatagacaac aagaaattgt tgttaaaggt gctaatgaaa caagatcata
     8761 tcttgcttca ggtacttcaa gaattattgt agaaatcggt caaccagtac aacgtggtga
     8821 agtattaact gaaggttcta ttgaacctaa gaattactta tctgttgctg gattaaacgc
     8881 gactgaaagc tacttattaa aagaagtaca aaaagtttac cgtatgcaag gggtagaaat
     8941 cgacgataaa cacgttgagg ttatggttcg acaaatgtta cgtaaagtta gaattatcga
     9001 agcaggtgat acgaagttat taccaggttc attagttgat attcataact ttacagatgc
     9061 aaatagagaa gcatttaaac accgcaagcg ccctgcaaca gctaaaccag tattacttgg
     9121 tataactaaa gcatcacttg aaacagaaag tttcttatct gcagcatcat tccaagaaac
     9181 aacaagagtt ctaacagatg cagcaattaa aggtaagcgt gatgacttat taggtcttaa
     9241 agaaaacgta attatcggta agctaattcc agctggtact ggtatgagac gttatagcga
     9301 cgtaaaatac gaaaaaacag ctaaaccagt tgcagaagtt gaatctcaaa ctgaagtaac
     9361 ggaataacaa gtatataaca gaggctaagg ctttagcctc ttgttatttt tatgtaaatt
     9421 atttgattta atgttgacga attctcttgt tcaatgttaa tatattaaag gttgatgcaa
     9481 gcagaacttt ggaggataaa ttattgtcta aggaaaaagt tgcacgcttt aacaaacaac
     9541 attttgtagt tggtcttaaa gaaacgctta aagcgttaaa gaaagatcaa gttacatctt
     9601 tgattattgc tgaagacgtt gaagtatatt taatgactcg cgtgttaagc caaatcaatc
     9661 agaaaaatat acctgtatct tttttcaaaa gcaaacatgc tttgggcaaa tatgtaggta
     9721 ttaacgtcaa tgcgacaata gtagcattga ttaaatgaga attagtaagt gttttactta
     9781 ctaaatttta tttaacctaa aaatgaacca cctggatgtg tgggattaaa aagtgaagag
     9841 aggaggacat atcacatgcc aactattaac caattagtac gtaaaccaag acaaagcaaa
     9901 atcaaaaaat cagattctcc agctttaaat aaaggtttca acagtaaaaa gaaaaaattt
     9961 actgacttaa actcaccaca aaaacgtggt gtatgtactc gtgtaggtac aatgacacct
    10021 aaaaaaccta actcagcgtt acgtaaatat gcacgtgtgc gtttatcaaa caacatcgaa
    10081 attaacgcat acatccctgg tatcggacat aacttacaag aacacagtgt tgtacttgta
    10141 cgtggtggac gtgtaaaaga cttaccaggt gtgcgttacc atattgtacg tggagcactt
    10201 gatacttcag gtgttgacgg acgtagacaa ggtcgttcat tatacggaac taagaaacct
    10261 aaaaactaag aatttagttt ttaattaaat cttaaactta aaatatt
//
LOCUS       DAPIDM010000088        10218 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000088 DAPIDM010000000
VERSION     DAPIDM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.088"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            140..1501
                     /gene="dnaA"
                     /locus_tag="RJE42_001854"
     CDS             140..1501
                     /gene="dnaA"
                     /locus_tag="RJE42_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV5877268.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATSKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            1779..2912
                     /gene="dnaN"
                     /locus_tag="RJE42_001855"
     CDS             1779..2912
                     /gene="dnaN"
                     /locus_tag="RJE42_001855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV5877269.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            3294..3539
                     /gene="yaaA"
                     /locus_tag="RJE42_001856"
     CDS             3294..3539
                     /gene="yaaA"
                     /locus_tag="RJE42_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDV5877270.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            3536..4648
                     /gene="recF"
                     /locus_tag="RJE42_001857"
     CDS             3536..4648
                     /gene="recF"
                     /locus_tag="RJE42_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV5877271.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     ETLSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSIDGIDHEIMNNAKLYRINQGEIIK"
     gene            4658..6592
                     /gene="gyrB"
                     /locus_tag="RJE42_001858"
     CDS             4658..6592
                     /gene="gyrB"
                     /locus_tag="RJE42_001858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV5877272.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGDIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            6629..9298
                     /gene="gyrA"
                     /locus_tag="RJE42_001859"
     CDS             6629..9298
                     /gene="gyrA"
                     /locus_tag="RJE42_001859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDV5877273.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNVGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDADDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(9383..10195)
                     /locus_tag="RJE42_001860"
     CDS             complement(9383..10195)
                     /locus_tag="RJE42_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDV5877274.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         3631 a         1571 c         1963 g         3053 t
ORIGIN      
        1 cataactact aagcaggata aagttatcca ccgattgtta ttaacttgtg gataattatt
       61 aacatggtgt gtttagaagt tatccacggc tgttattttt gtgtataact taaaaattta
      121 agaaagatgg agtaaattta tgtcggaaaa agaaatttgg gaaaaagtac ttgaaattgc
      181 tcaagaaaaa ttatcagctg taagttactc aactttccta aaagatactg agctttacac
      241 gattaaagat ggtgaagcta tcgtattatc gagtattcct tttaatgcaa attggttaaa
      301 tcaacaatat gctgaaatta tccaagcaat cttatttgat gttgtaggct atgaagtaaa
      361 acctcacttt attactactg aagaattagc aaattatagt aataatgaaa ctgctacttc
      421 aaaagaaaca acaaaacctt ctactgaaac aactgaggat aatcatgtgc ttggtagaga
      481 gcaattcaat gcccataaca catttgacac ttttgtaatc ggacctggta accgctttcc
      541 acatgcagcg agtttagctg tggccgaagc accagccaaa gcgtacaatc cattatttat
      601 ctatggaggt gttggtttag gaaaaaccca tttaatgcat gccattggtc atcatgtttt
      661 agataataat ccagatgcca aagtgattta cacatcaagt gaaaaattca caaatgaatt
      721 tattaaatca attcgtgata acgaaggtga agctttcaga gaaagatatc gtaatatcga
      781 cgtcttatta atcgatgata ttcagttcat acaaaataaa gtacaaacac aagaagaatt
      841 tttctatact tttaatgaat tgcatcagaa taacaagcaa atagttattt cgagtgatcg
      901 accaccaaag gaaattgcac aattagaaga tcgattacgt tcgcgctttg aatgggggct
      961 aattgttgat attacgccac cagattatga aactcgaatg gcaattttgc agaagaaaat
     1021 tgaagaagaa aaattagata ttccaccaga agctttaaat tatatagcaa atcaaattca
     1081 atctaatatt cgtgaattag aaggtgcatt aacgcgttta cttgcatatt cacaattatt
     1141 aggaaaacca attacaactg aattaactgc tgaagcttta aaagatatca ttcaagcacc
     1201 aaaatctaaa aagattacca tccaagatat tcaaaaaatt gtaggccagt actataatgt
     1261 tagaattgaa gatttcagtg caaaaaaacg tacaaagtca attgcatatc cacgtcaaat
     1321 agctatgtac ttgtctagag agcttacaga tttctcatta cctaaaattg gtgaagaatt
     1381 tggtgggcgt gatcatacga ccgtcattca tgctcatgaa aaaatatcta aagatttaaa
     1441 agaagatcct atttttaaac aagaagtaga gaatcttgaa aaagaaataa gaaatgtata
     1501 agtaggaaac ttcgggaaat gtaatctgtt atataacagc actaataata acaatcattt
     1561 tttacatttc tatatgctaa tgtggcaaga tgagcaaaac tcattttgtg gataatgttt
     1621 aaaagtcata cataccatac acaagttatc aacatgtgta taacttcgcc aaatctatgt
     1681 ttttaagact tatccaccaa tccacagcac ctactactat tactaagaac ttaaaaccta
     1741 tataattata tataaacgac tggaaggagt tttaattaat gatggaattc actattaaaa
     1801 gagattattt tattacacaa ttaaatgaca cattaaaagc tatttcacca agaacaacat
     1861 tacctatatt aactggtatc aaaatcgatg caaaagaaca tgaagttata ttaactggtt
     1921 cagactctga aatttcaata gaaatcacta ttcctaaaac tgtagatggc gaagatattg
     1981 tcaatatttc agaaacaggg tcagtagtac ttcctggacg attctttgtt gatattataa
     2041 aaaaattacc tggtaaagat gttaaattat ctacaaatga acaattccag acattaatta
     2101 catcaggtca ttctgaattt aatttaagtg gcttagatcc agatcaatat cctttattac
     2161 ctcaagtttc tagagatgac gcaattcaat tgtcggtaaa agtgcttaaa aacgtaattg
     2221 cacaaacgaa ttttgcagtg tccacctcag aaacacgccc agtactaact ggtgtgaact
     2281 ggcttataca agaaaacgaa ttaatatgca cagcgactga ctcacaccgc ttggctgtaa
     2341 gaaagttgca gttagaagat gtttctgaaa acaaaaatgt catcattcca ggtaaggctt
     2401 tagctgaatt aaataaaatt atgtctgaca atgaagaaga cattgatatc ttctttgctt
     2461 caaaccaagt tttatttaaa gtcggaaatg taaactttat ttctcgatta ttagaaggac
     2521 attatcctga tacaacacgt ttattccctg aaaactatga aattaaatta agtatagaca
     2581 atggggagtt ttatcatgcg attgatcgtg catctttatt agcacgtgaa ggtggtaata
     2641 acgttattaa attaagtaca ggtgatgacg ttgttgaatt gtcttctaca tcaccagaaa
     2701 ttggtactgt aaaagaagaa gttgatgcaa acgatgttga aggtggtagc ctgaaaattt
     2761 cattcaactc taaatatatg atggatgctt taaaagcaat cgataatgat gaggttgaag
     2821 ttgaattctt cggtacaatg aaaccattta ttctaaaacc aaaaggtgac gactcggtaa
     2881 cgcaattaat tttaccaatc agaacttact aaaaataaat ataaataaag gatgacgtga
     2941 ttaattaaaa cgtcatcctt tattttttgg caaaaataat tctagattcg tatgtaaaat
     3001 aaatttgaca gcattttaaa cagcaaataa aagacgccaa ttaaatttat gacaaaatgt
     3061 atccagaatt taataagtgt gcttatatgc cctttaaatt taaaatttta atagtcaata
     3121 acaagttgaa tataaaagtt aaacgccgtt aaatagcgtt taaaaaatga aaatgacagt
     3181 attgccaaaa aataagaatt aattattcat atgtaaacgg tttctacctc tattttaaat
     3241 gaaatttgtg acaaaaaaag gtataatata ttaatgacat acaaagaaat ggagtgatta
     3301 ttttggttca agaagttgta gtagaaggag acattaattt aggtcaattt ctaaaaacag
     3361 aagggattat tgaatctggt ggtcaagcaa aatggttctt gcaagacgtt gaagtattaa
     3421 ttaatggagt gcgtgaaaca cgtcgcggta aaaagttaga acatcaagat cgtatagata
     3481 tcccagaatt acctgaagat gctggttctt tcttaatcat tcatcaaggt gaacaatgaa
     3541 gttaaataca ctccaattag aaaattatcg taactatgat gaggttacgt tgaaatgtca
     3601 tcctgacgtg aatatcctca ttggagaaaa tgcacaagga aagacaaatt tacttgaatc
     3661 aatttatacc ttagctttag caaaaagtca tagaacgagt aatgataagg aactcatacg
     3721 ttttaatgct gattatgcta aaatagaagg tgagcttagt tatagacacg gcacgatgcc
     3781 attaacaatg tttataacta aaaaaggtaa acaagtcaaa gtgaatcact tagagcaaag
     3841 tcgtctaact caatatattg gacacctcaa tgtggttcta tttgcgccag aagatttgaa
     3901 tattgtaaaa ggctctcctc aaataagacg acgctttata gatatggagt tgggccaaat
     3961 ttctgctgtt tacttaaatg atttagctca ataccaacgt attttaaagc aaaagaataa
     4021 ttacttaaag cagttacaat taggccaaaa aaaggactta acaatgttgg aagtattaaa
     4081 tcagcagttt gctgaatatg caatgaaagt aactgataaa cgtgcacatt ttattcaaga
     4141 gctagagtcg ttagctaaac cgattcatgc tggtatcaca aatgataaag aaacgttgtc
     4201 gctgaattat ttacctagtc ttaaatttga ttatgctcaa aatgaagcgg cacgacttga
     4261 agaaattatg tctattctta gcgataatat gcaaagagaa aaagaacgag gcattagctt
     4321 attcggacca catcgagatg atataagttt tgatgtgaat ggcatggatg ctcaaacata
     4381 tggttctcaa ggacagcaac gtacaacggc tttgtccatt aaattagctg aaattgagtt
     4441 aatgaatatc gaagttgggg aatatcctat cttattatta gacgatgtac tcagtgaatt
     4501 agatgattcg cgtcaaacgc atttattaag tacgattcag cataaagtac aaacatttgt
     4561 cactacgaca tctatagatg gtattgatca tgaaatcatg aataacgcta aattgtatcg
     4621 tattaatcaa ggtgaaatta taaagtaaca gaaagcgatg gtgactgcat tgtcagatgt
     4681 aaacaacacg gataattatg gtgctgggca aatacaagta ttagaaggtt tagaagcagt
     4741 acgtaaaaga ccaggtatgt atataggatc gacttcagag agaggtttgc accatttagt
     4801 gtgggaaatt gtcgataata gtatcgatga agcattagct ggttatgcaa atcaaattga
     4861 agttgttatt gaaaaagata actggattaa agtaacggat aacggacgtg gtatcccagt
     4921 tgatattcaa gaaaaaatgg gacgtccagc tgtcgaagtt attttaactg ttttacatgc
     4981 tggtggtaaa tttggcggtg gcggatacaa agtatctggt ggtttacatg gtgttggttc
     5041 atcagttgta aacgcattgt cacaagactt agaagtatat gtacacagaa atgagactat
     5101 atatcatcaa gcatataaaa aaggtgtacc tcaatttgac ttaaaagaag ttggcacaac
     5161 tgataagaca ggtactgtca ttcgttttaa agcagatgga gatatcttca cagagacaac
     5221 tgtatacaac tatgaaacgt tacaacaacg tattagagag cttgctttct taaacaaagg
     5281 aatacaaatc acattaagag atgaacgtga tgaagaaaac gttagagaag actcctatca
     5341 ctatgagggc ggtattaaat cttatgttga gttattgaac gaaaataaag aacctattca
     5401 tgatgagcca atttatattc atcaatctaa agatgatatt gaagtagaaa ttgcgattca
     5461 atataactca ggatatgcca caaatctttt aacttacgca aataacattc atacgtatga
     5521 aggtggtacg catgaagacg gattcaaacg tgcattaacg cgtgtcttaa atagttatgg
     5581 tttaagtagc aagattatga aagaagaaaa agatagactt tctggtgaag atacacgtga
     5641 aggtatgaca gcaattatat ctatcaaaca tggtgatcct caattcgaag gtcaaacgaa
     5701 gacaaaatta ggtaattctg aagtgcgtca agttgtagat aaattattct cagagcactt
     5761 tgaacgattt ttatatgaaa atccacaagt cgcacgtaca gtggttgaaa aaggtattat
     5821 ggcggcacgt gcacgtgttg ctgcgaaaaa agcgcgtgaa gtaacacgtc gtaaatcagc
     5881 attagatgta gcaagtcttc caggtaaatt agccgattgc tctagtaaaa gtcctgaaga
     5941 atgtgagatt ttcttagtcg aaggggactc tgctgggggg tctacaaaat ctggtcgtga
     6001 ctctagaacg caggcgattt taccattacg aggtaagata ttaaatgttg aaaaagcacg
     6061 attagataga attttgaata acaatgaaat tcgtcaaatg atcacagcat ttggtacagg
     6121 aatcggtggc gactttgatc tagcgaaagc aagatatcac aaaatcgtca ttatgactga
     6181 tgccgatgtg gatggagcgc atattagaac attgttatta acattcttct atcgatttat
     6241 gagaccgtta attgaagcag gctatgtgta tattgcacag ccaccgttgt ataaactgac
     6301 acaaggtaaa caaaagtatt atgtatacaa tgatagggaa cttgataaac ttaaatctga
     6361 attgaatcca acaccaaaat ggtctattgc acgatacaaa ggtcttggag aaatgaatgc
     6421 agatcaatta tgggaaacaa caatgaaccc tgagcaccgc gctcttttac aagtaaaact
     6481 tgaagatgcg attgaagcgg accaaacatt tgaaatgtta atgggtgacg ttgtagaaaa
     6541 ccgtagacaa tttatagaag ataatgcagt ttatgcaaac ttagacttct aagcgctgtg
     6601 aactgaactt ttgaaggagg aactcttgat ggctgaatta cctcaatcaa gaataaatga
     6661 acgaaatatt accagtgaaa tgcgtgaatc atttttagat tatgcgatga gtgttatcgt
     6721 tgctcgtgca ttgccagatg ttcgtgacgg tttaaaacca gtacatcgtc gtatactata
     6781 tggattaaat gaacaaggta tgacaccgga taaatcatat aaaaaatcag cacgtatcgt
     6841 tggtgacgta atgggtaaat atcaccctca tggtgactta tctatttatg aagcaatggt
     6901 acgtatggct caagatttca gttatcgtta tccgcttgtt gatggccaag gtaactttgg
     6961 ttcaatggat ggagatggcg cagcagcaat gcgttatact gaagcgcgta tgactaaaat
     7021 cacacttgaa ctgttacgtg atattaataa agatacaata gattttatcg ataactatga
     7081 tggtaatgaa agagagccgt cagtcttacc tgctcgattc cctaacttgt tagccaatgg
     7141 tgcatcaggc atcgcggtag gtatggcaac gaatattcca ccacataact taacagaatt
     7201 aatcaatggt gtacttagct taagtaagaa ccctgatatt tcaattgctg agttaatgga
     7261 agatattgaa ggtcctgatt tcccaactgc tggacttatt ttaggtaaga gtggtattag
     7321 acgcgcatat gaaacaggtc gtggttcaat tcaaatgcgt tctcgtgcag ttattgaaga
     7381 acgtggaggc ggacgtcaac gtattgttgt cactgaaatt cctttccaag tgaataaggc
     7441 tcgtatgatt gaaaaaattg cagagctcgt tcgtgacaag aaaattgacg gtatcactga
     7501 tttacgtgat gaaacaagtt tacgtactgg tgtgcgtgtc gttattgatg tgcgtaagga
     7561 tgcaaatgct agtgtcattt taaataactt atacaaacaa acacctcttc aaacatcatt
     7621 tggtgtgaat atgattgcac ttgtaaatgg tagaccgaag cttattaatt taaaagaagc
     7681 gttggtacat tatttagagc atcaaaagac agttgttaga agacgtacgc aatataacct
     7741 acgtaaagct aaagatcgtg cccacatttt agaagggtta cgtatcgcac ttgaccatat
     7801 cgatgaaatt atttcaacga ttcgtgagtc agatacagat aaagttgcga tggaaagctt
     7861 gcaacaacgc ttcaaacttt ctgaaaaaca agctcaagct attttagaca tgcgtttaag
     7921 acgtctaaca ggtttagaga gagacaaaat tgaagctgaa tataatgagt tattaaatta
     7981 tattagtgaa ttagaagcaa tcttagctga tgaagaagtg ttattacagt tagttagaga
     8041 tgaattgact gaaattagag atcgtttcgg tgatgatcgt cgtacagaaa ttcaattagg
     8101 tggatttgaa gacttagagg acgaagactt aattccagaa gaacaaatag taattacttt
     8161 gagccataat aactacatta aacgtttgcc ggtatctaca tatcgtgctc aaaaccgtgg
     8221 tggtcgtggt gttcaaggta tgaatacatt ggaagaagat tttgtcagtc aattggtaac
     8281 tttaagtaca catgaccatg tattgttctt tactaacaaa ggtcgtgtat acaaactaaa
     8341 aggttatgaa gtgcctgagt tatcaagaca gtctaaaggt attcctgtag tgaatgctat
     8401 tgaacttgaa aatgatgaag tcattagtac aatgattgct gttaaagacc ttgaaagtga
     8461 agacaacttc ttagtgtttg caactaaacg tggtgtcgtt aaacgttcag cattaagtaa
     8521 cttctcaaga ataaatagaa atggtaagat tgcgatttcg ttcagagaag atgatgagtt
     8581 aattgcagtt cgcttaacaa gtggtcaaga agatatcttg attggtacat cacatgcatc
     8641 attaattcga ttccctgaat caacattacg tcctttaggc cgtacagcta cgggtgtgaa
     8701 aggtattaca cttcgtgaag gtgacgaagt tgtagggctt gatgtagctc atgcaaacag
     8761 tgttgatgaa gtattagtag ttactgaaaa tggttatggt aaacgtacgc cagttaatga
     8821 ctatcgttta tcaaatcgtg gtggtaaagg tattaaaaca gctacgatta ctgagcgtaa
     8881 tggtaatgtt gtatgtatca ctacagtaac tggtgaagaa gatttaatga ttgttactaa
     8941 tgtcggtgtc attattcgac tagatgttgc agatatttct caaaatggtc gtgcagcaca
     9001 aggtgttcgc ttaattcgct taggcgatga tcaatttgtt tcaacggttg ctaaagtaaa
     9061 agaggatgca gacgatgaaa cgaatgaaga tgagcaatct acttcaactg tatctgaaga
     9121 tggtactgaa caacaacgtg aagcggttgt aaatgatgaa acaccaggaa atgcaattca
     9181 tactgaagtg attgattcag aagaaaatga tgaagatgga cgtattgaag taagacaaga
     9241 tttcatggat cgtgttgaag aagatataca acaatcatca gatgaagatg aagaataaaa
     9301 aaataagact tccctatatg taggggagtc ttatttttat gctagaaagt aatgctgtac
     9361 tatattcaat gagtagtaat gactaacttt ctaattgttt cattgcataa ggtatttcat
     9421 tgataagtct tgatggtggc accacataca tatcttttgc aagattttcg ccaataaaac
     9481 tatgtgtata tgtggcactc ataactgctt cttttaagtt atcaaattga ccaacaaaac
     9541 ttgtaatcat accagcaagt gtatcgccca taccaccagt cgccattgct gggctaccga
     9601 ttgtcaattt aaagtcttca tctttaaaga aaatttcagt accgtgtttt ttaagtacaa
     9661 cagttgcgcc taaacgatca actgcttcac gattacgctc atatgtctgt tcctcaatag
     9721 gaataccact taatcgttcc cattctttga ggtgcggtgt aaagatcaca cgacatgtag
     9781 gtaattgcgg tttcagttta ctaaatattg taatcgcatc gccgtctacg attaaatttt
     9841 gatgcggttg tatattttgt agtaggaatg taatggcatt atttcctttg aaatcaacgc
     9901 caagacctgg accaattagt atactgtcag tcatttcaat cattttcgtc aacattttcg
     9961 tatcattaat atcaataacc atcgcttctg ggcaacgaga atgtaatgct gaatgatttg
    10021 ttggatgtgt agctacagtg attaaaccac taccgctaaa tacacatgca cgagccgcta
    10081 acataatggc accacctaag ttagcagatc caccaattaa taaaattttg ccataatcac
    10141 ctttatgtga atcttcttta cgcttaggaa tgttaataga atttaacgtt tccatagtga
    10201 tataacctcc catataaa
//
LOCUS       DAPIDM010000089        10214 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000089 DAPIDM010000000
VERSION     DAPIDM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.089"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            61..873
                     /gene="tagG"
                     /locus_tag="RJE42_001861"
     CDS             61..873
                     /gene="tagG"
                     /locus_tag="RJE42_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDV5877275.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            972..2075
                     /locus_tag="RJE42_001862"
     CDS             972..2075
                     /locus_tag="RJE42_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDV5877276.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKSFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKEDNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTVEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            2072..3133
                     /locus_tag="RJE42_001863"
     CDS             2072..3133
                     /locus_tag="RJE42_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV5877277.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMEIKMTDPHDSDTFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDVTLANTYNHEILVKAMQKATDIHLVSQMIVGDNDIDTHATSNNE
                     DFKRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFNGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            3196..3594
                     /gene="tagD"
                     /locus_tag="RJE42_001864"
     CDS             3196..3594
                     /gene="tagD"
                     /locus_tag="RJE42_001864"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDV5877278.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(3711..5006)
                     /gene="pbp4"
                     /locus_tag="RJE42_001865"
     CDS             complement(3711..5006)
                     /gene="pbp4"
                     /locus_tag="RJE42_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDV5877279.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWAEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            5427..7154
                     /locus_tag="RJE42_001866"
     CDS             5427..7154
                     /locus_tag="RJE42_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877280.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            7513..8742
                     /locus_tag="RJE42_001867"
     CDS             7513..8742
                     /locus_tag="RJE42_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV5877281.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNAKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIGGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            8997..9089
                     /locus_tag="RJE42_001868"
     CDS             8997..9089
                     /locus_tag="RJE42_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877282.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            9267..10100
                     /locus_tag="RJE42_001869"
     CDS             9267..10100
                     /locus_tag="RJE42_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDV5877283.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPEKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
BASE COUNT         3492 a         1435 c         1841 g         3446 t
ORIGIN      
        1 tataatacta tttagttaag tagagaaata gttaaacatt tgaaagtgtg gtttaatgga
       61 atgtcagcaa taggaacagt ttttaaagaa catgtaaaga acttttattt aattcaaaga
      121 ctggctcagt ttcaagttaa aattatcaat catagtaact atttaggtgt ggcatgggaa
      181 ttaattaacc ctgttatgca aattatggtt tactggatgg tttttggatt aggaataaga
      241 agtaatgcac caattcatgg tgtacctttt gtttattggt tattggttgg tatcagtatg
      301 tggttcttca tcaaccaagg tattttagaa ggtactaaag caattacaca aaagtttaat
      361 caagtatcga aaatgaactt cccgttatcg ataataccga catatattgt gacaagtaga
      421 ttttatggac atttaggctt acttttactt gtgataattg catgtatgtt tacgggtatt
      481 tatccatcaa tacatatcat tcaattattg atatatgtac cgttttgttt tttcttaact
      541 gcctcggtga cgttattaac atcaacactc ggtgtgttag ttagagatac acaaatgtta
      601 atgcaagcaa tattaagaat attattttac ttttcaccaa ttttgtggct accaaagaac
      661 catggtatca gtggtttaat tcatgaaatg atgaaatata atccagttta ctttatcgct
      721 gaatcatacc gtgcagcaat tttatatcac gaatggtatt tcatggatca ttggaaatta
      781 atgttataca atttcggtat tgttgccatt ttctttgcaa ttggtgcgta cttacacatg
      841 aaatatagag atcaatttgc agacttcttg taatatattt atatgacgaa accccgctaa
      901 ccattaataa atggaagtgg ggttcatttt tgtttataat ttaagtaaat aacatattaa
      961 gttggtgtat tatgaacgtt ttaataaaga aattttatca tttggtagtt cgaatacttt
     1021 ctaaaatgat tacgcctcaa gtgattgata aaccgcatat cgtatttatg atgacttttc
     1081 cagaagatat taagcctatc atcaaagcat taaataattc gtcgtatcag aaaactgttt
     1141 taacaacacc aaaacaagcg ccttatttat ctgaacttag cgacgatgtt gatgtgatag
     1201 aaatgactaa tcgaacattg gtaaaacaaa ttaaggcttt gaaaagcgcg cagatgatta
     1261 ttatcgataa ttattaccta ttactaggtg gatataataa gacttctaat caacacattg
     1321 ttcaaacgtg gcatgcaagt ggtgcattaa aaagctttgg cttaacagat catcaagtcg
     1381 atgtgtctga caaggcaatg gttcagcagt accgtaaagt ttatcaagcg acggattttt
     1441 acttagtggg ttgtgaacaa atgtcacaat gttttaaaca gtctttaggt gcaacagaag
     1501 agcaaatgtt gtattttggg cttccgagaa ttaataaata ttacacagct gatagagcaa
     1561 cggttaaggc agagttaaag gataaatatg gcattacaaa taagttggca ttatatgtac
     1621 caacttatag agaagataaa gaagataata gggctattga taaagcttat tttgaaaaat
     1681 gtttaccagg atatacactg attaataaat tacatccatc aattgaacat tcagacattg
     1741 atgacgtatc ttcaatcgac acatctacat taatgttaat gtcagatata attattagcg
     1801 actatagttc gctgccaata gaagctagct tgttagatat tccaactata ttttatgtgt
     1861 atgatgaagg aacatatgat aaggtgagag gcctgaatca attttacaaa gcaattccgg
     1921 atagctataa agtgtatact gtagaagatt taataatgac gatacaagaa aaagaacatc
     1981 tattaagtcc gttatttaaa gattggcata agtataatac tgataaaagt ttacatcagc
     2041 tcacagaata tatagataag atggtgacaa aatgaggctt acgataatca tacctacatg
     2101 taataatgag gcaacaattc gacaattgtt aatctctatt gagagtaaag aacactatag
     2161 aatcctttgt attgatggtg gttctactga tcaaacaatt cctatgattg aacggttaca
     2221 aagagaactc aagcatattt cattaataca attacaaaat gcttcgatag ctacgtgtat
     2281 taataaaggt ttgatggaga tcaaaatgac agatccacat gatagtgaca catttatggt
     2341 cataaatcca acatcaatcg tattgccagg taaattagat aggctaactg cagcgttcaa
     2401 aaataatgat aatattgata tggtaatagg gcagcgagct tacaattacc atggtgaatg
     2461 gaaattgaaa agtgctgatg agtttattaa agacaatcga atcgttacat taacggaaca
     2521 accagatttg ttatcaatga tgtcttttga cggaaagtta ttcagtgcta aatttgctga
     2581 attacagtgt gacgtaactt tagctaacac ttataatcac gaaatacttg tcaaagcgat
     2641 gcaaaaagct acggatatac atttagtttc acagatgatt gtcggagata acgatataga
     2701 tacacatgct acaagtaaca atgaagattt taaaagatat atcacagaaa ttatgaaaat
     2761 aagacaacga gtcatggaaa tgttactatt acctgaacaa aggctattat atagtgatat
     2821 ggttgatcgt attttattca ataattcatt aaaatattat atgaatgaac acccagcagt
     2881 aacgcacacg acaattcaac ttgtaaaaga ctatattatg tctatgcagc attctgatta
     2941 tgtatcacaa aatatgtttg acattataaa tacagttgaa tttaatggtg agaattggga
     3001 tagagaaata tacgaattgt ggcgacaaac attaattcaa gtgggcatta ataggccgac
     3061 atataaaaga ttcttgatac aacttaaagg gagaaagttt gcacatcgaa caaaatcaat
     3121 gttaaaacga taatgtgtac attgatgacc ataagctgca atcctatgat gtgacaatat
     3181 gaggaggata acttaatgaa acgtgtaata acatatggca catatgactt acttcactat
     3241 ggtcatatcg aattgcttcg tcgtgcaaga gagatgggcg attatttaat agtagcatta
     3301 tcaactgatg aatttaatca aattaaacat aaaaaatctt attatgatta tgaacaacga
     3361 aaaatgatgc ttgaatcaat acgctatgtc gatttagtca ttccagaaaa gggttgggga
     3421 caaaaagaag acgatgtcga aaaatttgat gtagatgttt ttgttatggg acatgactgg
     3481 gaaggtgaat tcgacttctt aaaggataaa tgtgaagtca tttatttaaa acgtacagaa
     3541 ggcatttcga cgactaaaat caaacaagaa ttatatggta aagatgctaa ataaattata
     3601 tagaactatc gatagtaaac gataaattaa cgtaggttat tataaaataa atataaaacg
     3661 gacaagtttc gcagctttat aatgtgcaac ttgtccgctt ttagtatgtt ttattttctt
     3721 tttctaaata aacgattgat tatcatatga acaattagtg ctaatccagc gactaggcat
     3781 gcaccaccaa tgatagtgaa taatggatgt tctgcccaca tacttttagc aacagtattt
     3841 gccttttgga taattggctg atgaacttct acagttggag gtccataatc tttattaata
     3901 aattctcttg gatagtccgc gtgtacttta ccatcttcga ctacaagttt ataatctttt
     3961 ttactaaaat cacttggtaa aacatcgtaa agatcatttt caacataata tttcttacca
     4021 tttatccttt gctcaccttt agacaatatt tttacatatt tatactgatc aaatgaacgt
     4081 tccattaatg cattccccat catattacgt tgcttctcgc caccaaggtt tttatagtct
     4141 cctgcaccca tgataacttg attaattcta aatttacctc gtttagtagt aatcgtatgg
     4201 ttgtaatttg ctgtatcact tgatccagtt tttaaaccat ctgtaccagg caaactcatt
     4261 tttgcacctt ccaatgaaaa gttgaatgtg taatacgtaa ctgcatgcgt tgttggtgct
     4321 aactgctttg taaagtctaa tattttaggt gtctctttaa tcacgtgtaa atctaaaatg
     4381 gcgtagtctc tagcagtcgt tacagtacgt tcttggtctt tatactttgt tggtgcaaat
     4441 gtacgtaatc ttgaattttc agcacccgtt ggattgacga aatgtgtatt tttcattccg
     4501 atagctttag ctttgttatt cattaaatca acgaaatcgc tggtgttttt tgataccttc
     4561 ttagctaaaa ttaatgccgc ggcattacta gaattagata ctgtaatttg taataggtct
     4621 gcgattgtcc atacttgtcc aggatatagt ttcgtattac tcaactcagg tagtgtagac
     4681 ataatatatt ctttgttcgt cattgtgaca gtatcatcaa gtgaaagctg ccccttattt
     4741 acagcttcca atgttaagta cattgtcatt aatttagtca tagacgctgg attccactta
     4801 gtatcgatat tgtattgata cagtaattgt ccagtttgac ttacattaac agcactcgtc
     4861 ggttcgtatg cagccgacaa acctgcataa ccatattgat ttgctgcttg cacaggggtt
     4921 acgtcactgt tagcagcttg tgcatatggt gtcataatac ttaatgttaa acataaaatg
     4981 atgataatag atattaaatt tttcataaag cgttaatctt cccttttcca attcttaaat
     5041 attccctaaa agcaatggtt attcctactt acggaaacca ttgctaattc acttcacctt
     5101 aattaaattg ttgaaaataa agttttctgc agttaatttg aaaaataatg caaatatatt
     5161 acgtgtgtag ctaaaggtgt tataatgttt gtacgaagag caaacttact caaaagcgat
     5221 taattttcat gttttaatat aaagactttg agaagttatt acaaaaaatg taatagaaat
     5281 attctatcat ataaatgtta tgagcggtat tttggggcaa cactttattt gatttttaaa
     5341 gttttgttgg gagaaagtat atgatagaaa tgcatgcatc tatctaaatg aattaactat
     5401 aaatttcaaa cagaagaggt aaaactatga aacgagaaaa tccattgttt ttcttattta
     5461 aaaaactatc atggccagtg ggtcttatcg ttgcagctat cactatttca tcactaggga
     5521 gcttaagtgg actattagtg ccactgttta ctggtcgaat tgtagataaa ttttccgtga
     5581 gccatatcaa ttggaatcta atcgcattat ttggtggtat ctttgtcatc aatgctttat
     5641 taagtggatt aggtttatat ttattaagta aaattggtga aaaaattatt tatgcgatac
     5701 gctcagtttt atgggagcat ataatacaat taaaaatgcc attctttgac aaaaatgaaa
     5761 gtggtcaatt aatgagtcga ttaactgacg atacgaaagt gataaatgaa tttatttcac
     5821 aaaagctacc taacttatta ccatcaatcg ttacattagt tgggtcacta atcatgttat
     5881 ttattttaga ttggaaaatg acattattaa catttataac gataccgata tttgttttaa
     5941 ttatgattcc tctaggacgt attatgcaaa agatatcgac aagtacacaa tctgaaattg
     6001 caaactttag tggtttgtta gggcgtgtcc taactgaaat gcgtcttgtt aaaatatcaa
     6061 atacagagcg acttgaatta gataatgcac ataaaaattt gaatgaaata tataaattag
     6121 gtttaaaaca ggctaaaatt gcggcagttg tacaaccaat ttcaggtatt gttatgttgc
     6181 taacaattgc aattatttta ggttttggtg cattagaaat tgcgacaggt gcaatcactg
     6241 caggtacatt aattgcaatg atattttatg ttattcaatt atcgatgcct ttaatcaatc
     6301 tttcaacgtt agttacagat tataaaaagg cagtcggtgc aagtagtaga atatacgaaa
     6361 tcatgcaaga acctattgaa ccgacagaag ctcttgaaga ttctgaaaat gtattaattg
     6421 atgacggtgt gttgtcattt gaacatgtag actttaaata tgatgtgaag aaaatattag
     6481 atgatgtgtc gttccaaatc ccacaagggc aagtgagtgc ttttgtaggt ccttctggat
     6541 ctggtaaaag tacgatattt aatttgatag aacgtatgta tgaaattgag tcaggtgata
     6601 ttaaatatgg ccttgaaagt gtctatgata tcccgttatc taagtggcga cgcaaaattg
     6661 gatatgttat gcaatcaaat tcgatgatga gtggtacaat tagagacaat attttatacg
     6721 gaattaatcg tcatgtttca gatgaagaac ttattaatta tgctaaatta gcgaactgtc
     6781 atgattttat catgcaattt gatgaaggat atgacacgct tgtaggtgaa cgaggattga
     6841 aactgtctgg cggacaacgt caacgtattg atattgctag aagttttgtt aaaaatcctg
     6901 atattttgtt acttgatgaa gcaacagcta atctcgatag tgaaagtgaa ttgaaaattc
     6961 aagaagcttt agaaacattg atggaaggta gaacaacgat tgtcattgcg catcgtttgt
     7021 ctacaattaa aaaagccggt caaattatat tcttagacaa aggacaggta acaggtaaag
     7081 gtacgcattc agaactgatg gcatcacatg cgaagtataa aaactttgta gtgtctcaaa
     7141 aattaacaga ttaattttat atatataagt aagcttggag caagtacaga tataccatcg
     7201 agaaaattaa agtgtggcac attgatggat atagatgtta ataaattgct tcaagctttt
     7261 gtctatttta aatcatgtga gaagttacga cataataatt cttaaattaa tgaaatcgat
     7321 attttaagaa aaaaatgctc atggtataat acaagttata agcaaacata catatattaa
     7381 atactgtagc cacgagtcat aattcttcat attttacata gcaatttaac tgattttaga
     7441 gtccacggta cagaagtttg atatttcaat gtttctaaat ttttaaaaaa ttaaatcata
     7501 ggtgggtgcc aaatgttttt attaatcaac attattggtc taattgtatt tcttggtatt
     7561 gcggtattat tttcaagaga tcgcaaaaat atccaatggc aatcaattgg gatcttagtt
     7621 gttttaaacc tgtttttagc atggttcttt atttattttg attggggtca aaaagcagta
     7681 agaggagcag ccaatggtat cgcttgggta gttcagtcag cacatgctgg tacaggtttt
     7741 gcatttgcaa gtttgacaaa tgctaaaatg atggatatgg ctgttgcagc cttattccca
     7801 attttattaa tagtgccatt atttgatatc ttaatgtact ttaatatttt accgaaaatt
     7861 attggaggta ttggttggtt actagctaaa gtaacaagac aacctaaatt cgagtcattc
     7921 tttgggatag aaatgatgtt tttaggaaat actgaagcat tagccgtatc aagtgagcaa
     7981 ctaaaacgta tgaatgaaat gcgtgtatta acaatcgcaa tgatgtcaat gagctctgta
     8041 tcgggagcta ttgtaggtgc gtatgtacaa atggtaccag gagaactggt actaacggca
     8101 attccactaa atatcgttaa cgcgattatt gtgtcatgct tgttgaatcc agtaagtgtt
     8161 gaagagaaag aagatattat ttacagtctt aaaaacaatg aagttgaacg tcaaccattc
     8221 ttctcattcc ttggagattc tgtattagca gcaggtaaat tagtattaat catcatcgca
     8281 tttgttatta gttttgtagc attagctgat ctatttgatc gttttatcaa tttgattaca
     8341 ggattgatag gaggatggat aggcataaaa ggtagtttcg gtttaaacca aattttaggt
     8401 gtgtttatgt atccatttgc gctattactc ggtttaccat atgatgaagc gtggttggta
     8461 gcacaacaaa tggctaagaa aattgttaca aatgaatttg ttgttatggg tgaaatttct
     8521 aaagatattg catcttatac accacaccat cgtgcggtta ttacaacatt cttaatttca
     8581 tttgcaaact tctcaacgat tggtatgatt atcggtacat tgaaaggaat cgttgataaa
     8641 aagacatcag actttgtatc taaatatgtg cctatgatgc tattatcagg tatcctagtt
     8701 tcattattaa cagcagcttt cgttggttta tttgcatggt aatatgtcga agagtgacta
     8761 cgataataca ttttaactaa aaaagatgtc caggcatgtc gtctattgat ataggtgaga
     8821 tgcttggact tttttattat tgatataaag atatttaaat atttttaaag ttaccgaaat
     8881 tgatgcaata taaaaaccaa gtgcacatgg taatacactt ggcttttatg ggaaatgaat
     8941 attattgtac atatgacagt aaggactagg tacagtcata gtacttcgag caaaatttgt
     9001 tttgttatta taaacaacac aaaggagata acttctctat tgaagaagtt aaaaacatta
     9061 tagcagacaa tgaaatgaaa gtaaattaaa aattcagaat atttttaatt attatattgt
     9121 gagtgatatt tattagggaa agctattctt catataaatt agttaaatag taaatctttg
     9181 ttagaactct ttccatagag ttgtgacagt atttttgttg tgctactatt tttatagtct
     9241 aataaatata caaaggggat ggtttcgtga ataaaacggt taaagattta atactagttg
     9301 tcttaggttc atttatcttt gctgcaggcg taaatgcatt tattatttct ggtaacttag
     9361 gtgaaggcgg ggttacaggt ttagcaatta ttttatatta tgcgtttcat atttcaccag
     9421 ccatcactaa cttcttggtc aacgcagtat tgattgccat aggttataaa tttttgagta
     9481 agagaagtat gtacttaact attcttgtaa caattcttat ttcaatattt ttgagtttaa
     9541 cagaatcatg gcaagtagaa actggaaaca gcattgtgaa tgccattttt ggtggtgtaa
     9601 gcgttggact aggaatcgga gtaattatcc ttgcaggcgg tacaacagca ggtacaacaa
     9661 ttttggcgag aattgcaacg aaatacctcg atgtaagcac gccatatgct ttgcttttct
     9721 tcgatatgat cgttgttgca atttcactta cagttattcc acttgataaa gtattagtaa
     9781 cagtaatatc actttatata ggaacaaaag tgatggaata tgtcatagaa ggtttaaaca
     9841 ctaaaaaagc tatgacgatt atctcaacta atcccgaaaa acttgccaaa gcaatagatg
     9901 agcaaattgg aagaggttta accattttaa acggacatgg ctattatacg cgtgaagaaa
     9961 aagatgtctt atacgttgtt atttctaaaa cacaagtttc aaaagcaaag cgattaatta
    10021 aacaaatcga taaagatgca ttcctcgtaa ttcatgatgt aagagatgtt tatggtaatg
    10081 gctttcttgc agatgaataa ttaaatggca tgagcacaca tacttaaata gaagtccacg
    10141 gacaagtttt tgaattatga agacttatct gtgggcgttt tttattttat aaaagtaata
    10201 tacaagacat gaca
//
LOCUS       DAPIDM010000090        10169 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000090 DAPIDM010000000
VERSION     DAPIDM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.090"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(293..433)
                     /locus_tag="RJE42_001870"
     CDS             complement(293..433)
                     /locus_tag="RJE42_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDV5877284.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
     gene            complement(430..999)
                     /gene="agrB"
                     /locus_tag="RJE42_001871"
     CDS             complement(430..999)
                     /gene="agrB"
                     /locus_tag="RJE42_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDV5877285.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMTILTFIAIGLIIKYAPAATKKKPIPVRLIKRKKYYAIIVSLIFFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            1235..1369
                     /locus_tag="RJE42_001872"
     CDS             1235..1369
                     /locus_tag="RJE42_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDV5877286.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(1971..2756)
                     /locus_tag="RJE42_001873"
     CDS             complement(1971..2756)
                     /locus_tag="RJE42_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDV5877287.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKGG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADVLTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(3064..3690)
                     /locus_tag="RJE42_001874"
     CDS             complement(3064..3690)
                     /locus_tag="RJE42_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDV5877288.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            3887..5131
                     /locus_tag="RJE42_001875"
     CDS             3887..5131
                     /locus_tag="RJE42_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877289.1"
                     /translation="MSYDWFKKMLLSTSMLILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNVTSPSVNSEMNNNETGTPHESNQAGNEGTGSNSRDANPDSNNVKPDSNNQNPSP
                     DSKPDPNNPNPGPNPKPDPDKPKPNPEPKPDPDKPKPNPEPKPDPDKPKPNPDPKPDP
                     DKPKPNPDPKPDPNPNPKPDPNKPNPNPSPNPNQPGDSNHSGGSKNGGTWNPNASDGS
                     NQGQWQPNGNQGNSQNHTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEVTDE
                     DIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGS
                     MPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMIVVF
                     SIMLFLFVKRNKKKNKNESQRR"
     gene            complement(5156..5899)
                     /locus_tag="RJE42_001876"
     CDS             complement(5156..5899)
                     /locus_tag="RJE42_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDV5877290.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     YTQIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            6074..6358
                     /gene="groES"
                     /locus_tag="RJE42_001877"
     CDS             6074..6358
                     /gene="groES"
                     /locus_tag="RJE42_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV5877291.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            6434..8050
                     /gene="groL"
                     /locus_tag="RJE42_001878"
     CDS             6434..8050
                     /gene="groL"
                     /locus_tag="RJE42_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV5877292.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMSGMM"
     gene            8591..8770
                     /locus_tag="RJE42_001879"
     CDS             8591..8770
                     /locus_tag="RJE42_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877293.1"
                     /translation="MATISITTDYKFTKKSAQKLIDAMEINENNSNVNKTNIKATKIK
                     STCEVENLLKDYRSN"
     gene            8767..9393
                     /locus_tag="RJE42_001880"
     CDS             8767..9393
                     /locus_tag="RJE42_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877294.1"
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNIVNSFEIISISGSESA
                     HDVDLFLKNKSIAFDRQNLSRTHLVFSQFKSKQILVGYFTISNKPLVFTKRMLDKISN
                     TLKKKLYQKGETHSGNDNLIIQGYLIAQIGKNYSEEALATKSINGNDLLTLAYEKVLE
                     GASIFGGSYIWIEYEDVDRLREFYRKFGFTEIKDHTSENNLKMAILKI"
     gene            complement(9932..>10169)
                     /locus_tag="RJE42_001881"
     CDS             complement(9932..>10169)
                     /locus_tag="RJE42_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDV5877295.1"
                     /translation="LSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIF
                     VMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
BASE COUNT         3737 a         1493 c         1682 g         3257 t
ORIGIN      
        1 acagaataat ctgtgaagtc ataatcaaca aaatagaata ccatttgatt ttgacaaaat
       61 acattataat aatgaatgaa gttaaaatga ttaaggacgc gctatcaaac attttaaata
      121 gaaataacga taatgttgaa attcctataa tgaaaaaata atcaagttta ctgtacttaa
      181 taccactaat tatagctggt attgtaaaca ttaatatcat ttgagttaat acgaataaaa
      241 caaaattata actatttaat aattccacct actatcacac tttctatttt tattattcgt
      301 gtaattgagt taattcttta ggtacttcaa cttcatccat tatgaagtcg catgtactat
      361 aagctgcgat gttaccaatg ttttttaaaa tcccagtaat aaaatcaaaa aataaattaa
      421 ataatgtatt cattttaagt cctccttaat aaagaaaata ggtagtaaag taatggcttc
      481 tattatgatg cctaattgaa taaattgggc aaatggttct ttgatgataa gtgtgataat
      541 gaaaaagatt aaactaacaa taattgcgta atattttttt cgcttaataa gtcgaacggg
      601 aataggtttc tttttagttg ctgcaggagc atatttaatt atcaaaccaa tagcaataaa
      661 cgtcaaaata gtcataatta aaaagttaat atgaaaattt actattacta aaggtaaaag
      721 tataaataat ataatacttt ctacataaca ccaaaaggaa gaaggtgcat gggcaccatg
      781 agcatgtctt cttattaaat aaaatgttaa attcgtaatt aacgtaaaga gaaaaatgtt
      841 taaaatatag gcaatcgtat acataacaat cagttttcct atatttttag ctaagacctg
      901 catccctaat cgtacttgca aaaattgaat atgatctaag ttatttctct tttgaagata
      961 cgtggcaaac tggtcaattt tattatcaaa ataattcaat tttacaccac tctcctcact
     1021 gtcattatac gatttagtac aatcttttat cattatattg cctaactgta ggaaataaat
     1081 acttaactgt taaatgtaat ttgtatttaa tattttaaca taaaaaaatt tacagttaag
     1141 aataaaaaac gactagttaa gaaaaaatgg aaaataaatg cttatagcat gttttaatat
     1201 aactagatca cagagatgtg atggaaaata gttgatgagt tgtttaattt taagaatttt
     1261 tatcttaatt aaggaaggag tgatttcaat ggcacaagat atcatttcaa caatcggtga
     1321 cttagtaaaa tggattatcg acacagtgaa caaattcact aaaaaataag atgaataatt
     1381 aattactttc attgtaaatt tgttatcttc gtatagtact aaaagtatga gttattaagc
     1441 catcccaact taataaccat gtaaaattag caagtgagta acatttgcta gtagagttag
     1501 tttccttgga ctcagtgcta tgtatttttc ttaattatca ttacagataa ttatttctag
     1561 catgtaagct atcgtaaaca acatcgattt atcattattt gataaataaa atttttttca
     1621 taattaataa catccccaaa aatagattga aaaaataact gtaaaacatt cccctaataa
     1681 taagtatggt cgtgagcccc tcccaagctc gcggcctttt tttattgtaa taaagtaggg
     1741 aagagttaat cctcattatg agatccaccg ttaaaatata tgaataagtc taatgttgga
     1801 aaaggtcaaa aaaataatca atttaattaa gaaaaacatt catttgctat gggcgaaact
     1861 ggttcttact gagttatcta ttataaaaaa ataaacatag gcttatgaaa aatcgtctca
     1921 taaatctatg tttagtcatg acatgtgtta aatattattt cgtgtgcttt ttatttatac
     1981 aaatctaatt taatactttt aaatacaggt atattttcgc gttgctgttc tacttcattt
     2041 aagtttaaat ctacagtcaa aacatctgcg gattcattta attctccaac taaatctccg
     2101 tttggattta taactatcga atgaccagca tattctgtgt taccatcgaa tccagtgcta
     2161 ttagttccaa taacaaacat attattttca attgcacgtg cctttagtaa agaatgccaa
     2221 tgttgaagac gcgacatagg ccattgtgcc acataaaatg caattttagc accactacga
     2281 gcaggatatc ttaataattc tggaaaacgt aaatcataac agataagttg ggtcacataa
     2341 gtaccgtcag acaattgaaa gggttcagct acgttttcgc cagcggttaa aaattcatgc
     2401 tctcttaaca taggaactaa atgaactttg tcgtattcat taatcagctg gccaccttta
     2461 ttcacactaa aagctgtatt aaatatttga ttgtttctaa tgttagaaac tgacccagct
     2521 acgatatcga ctttatattt ttcagctaaa tgtttaataa atgaaaaact ttgtcctaga
     2581 ttattatctg ctttttcatt taaatgctct aaatcatagc cattattcca catttcaggt
     2641 aaaacgacta catctacttc agcattcata tttttttcga accattgcgt tatttgggtt
     2701 tcatttttag aactatctcc aaaaataatc ggtaattgat aaatttggac tttcataaca
     2761 tcacatcctt gatagatctt atatataact tactaaaagt tatgttcaaa cgcaaaaaac
     2821 aagcccagga cataaaatca aagtcctagg ctctacaatg ttatattgac agtagttgtt
     2881 ggggtgggct ctaacataaa gaaatacttt ttctttagaa attagtattt cttatacatg
     2941 agttttactc atgtattcct attcttaagt acacattagc agcggctaat gtgtaagaac
     3001 tactacataa tgaataacta atgattctat atcatttctg tcccattccc aacaatatat
     3061 tgattatttt tttattacga aacgatcttc cactggatta aatgtttttt cgccagcagc
     3121 ttcacgaata tcaccaaatg gcatttgagc aataagtttc caacttttag gaatattaaa
     3181 ttcatttgaa gtcatctcat caacaagtgg attatagtgt tgtaatgaag cacctatgcc
     3241 tttagtagct aatgcagtcc aaattgcaaa ttgatgcatg gcatttgttt gagttgacca
     3301 tattgcaaaa ttatcatagt agtttggcat ttgttcttgt aaaccactta caacatcttg
     3361 atcttcataa aacaaaattg tgccgtatga atgtttgaag ttatcaattt tttgttcagt
     3421 tggctcgaaa tcacgattct ctcccatgac ttcttttaaa attgcttttg tgttatccca
     3481 aaatttatta ttgttgtcat ttaataagag aacaattcta gttgattgag aattaaatga
     3541 tgaaggaaca tgtttaactg catgtgcaat cattgattct aattcgtcat cgctaattga
     3601 tattgaatct ttcaaattat atattgaacg tctttcttcc attgcgttgt caaaagtcat
     3661 tgctttttta tcttttttaa ataaacccat aatcattact ccttctttag taaagaatac
     3721 ttaatagact tagtataaat tttatactcg aacttgtaaa gtaatattta cgaaaatttc
     3781 aagaatatta atattcattt tcaaattcca aatataaatg cattttcaac gcatatttat
     3841 tatacttaga ttaatactta tatgaaaaag ggaggtgtct cgtgaaatgt catatgattg
     3901 gtttaagaaa atgttacttt caacaagtat gttaatttta agtagtagta gtttagggct
     3961 tgcaacgcac acagttgaag caaaggataa cttaaatgga gaaaagccaa cgactaattt
     4021 gaatcataat gtaacttcac catcagtaaa tagtgaaatg aataataatg agactgggac
     4081 acctcacgaa tcaaatcaag ctggtaatga aggaactggt tcgaatagtc gtgatgctaa
     4141 tcctgattcg aataatgtga agccagactc aaacaaccaa aacccaagtc cagattcaaa
     4201 acctgaccca aataacccaa acccaggtcc gaatccgaag ccagacccag ataagccgaa
     4261 accaaatccg gaaccaaagc cagacccaga taaaccaaag ccaaatccgg aaccaaagcc
     4321 agacccagat aagccgaaac caaatccgga tccaaaacca gatccagaca aaccgaagcc
     4381 aaatccggat ccaaaaccag atccaaatcc gaatccaaaa ccagacccta ataagccaaa
     4441 tccaaatccg tctccaaatc ccaatcaacc tggggattcc aatcattctg gtggctcgaa
     4501 aaatggggga acatggaacc caaatgcttc agatggatct aatcaaggtc aatggcaacc
     4561 aaatggaaat caaggaaact cacaaaatca tactggtaat gattttgtat cccaacgatt
     4621 tttagccttg gcgaatgggg cttacaagta taatccgtat attttaaatc aaattaataa
     4681 gttgggcaaa gattatggag aagttactga tgaagacatt tataatatta ttcgcaaaca
     4741 aaacttcagc ggaaatgcat atttaaatgg attacaacag caatcgaatt actttagatt
     4801 ccaatatttc aatccattga aatcagaaag gtactatcgt aatttagatg aacaagtact
     4861 cgcattaatt actggtgaaa ttggatcaat gccagatttg aaaaagcccg aagataagcc
     4921 ggattcaaaa caacgttcat ttgagcctca tgaaaaagac gattttacag tagttaaaaa
     4981 acaagaagat aataagaaaa gtgcgtcaac tgcatatagt aaaagttggc tagcaattgt
     5041 atgttctatg attgtggtat tttcaatcat gctattctta tttgtaaagc gaaataaaaa
     5101 gaaaaataaa aacgaatcac agcgacgata atccgtgtgt gattcgtttt ttatattatg
     5161 gaataaaaat gtgatatata aaattcgctt gttccgtggc ttttttcaaa gcttcaggat
     5221 taagtaattg gaatataacg acaaatccat tttgtaacat atgaataata attggaacag
     5281 caagtcgttt cgtccataca tatgctaatg aaaaaatgac gcccatacca aagtatatag
     5341 gaataaattt atagtcatta tgcgctaatg caaagattaa tgagcttaca gtcgttgcaa
     5401 tagcaaatgc gacgatacgg ttacctttaa ttgcattaaa taattcgcca aagattactt
     5461 ttctaaatac atattcttct aatagaggac caacgattga tacgaaaaag ataaacaatg
     5521 gaatctttcg tgcaataacg attaaacgct cagtatttgg acttgtttgt tgtgtgccaa
     5581 aaatttgagt atatacaata ctaactacca tttgataaat cattaccagt gcaaatccaa
     5641 gcaatgccca tggaatgata tattttttag gttctttaac ttctaattct aattttgttg
     5701 gatttttaat ttttaaatta attaaaataa tcgtcgtggc ggcgattaaa aatagaacaa
     5761 gttgtatgta aatgactgct ttagtcagtt ctatgccact atattgtaca aatggtaatt
     5821 tttttacaat gagaagcggt aaaaattgag acaatatata aataataaca gttagcaatg
     5881 atgcccatag tcttgtcata attttcctcc aaatatttgt ttataattta ttttatcgta
     5941 aataacttga agttacaaaa cttaattgaa aggttatgac ttgaagtttt gaccaaattt
     6001 gattattata aatgtatgtt agcactctat aaagttaagt gctaaacttt aggtttttta
     6061 aggaggaaca atcatgctaa aaccaattgg aaatcgtgtg attattgaga aaaaagaaca
     6121 agaacaaaca actaaaagtg gtattgtttt aactgatagt gctaaagaaa aatcaaacga
     6181 aggcgttatc gttgcagtag gaacaggacg cctattaaat gatggtacaa gagtgactcc
     6241 tgaagtgaaa gaaggggacc gtgtcgtgtt ccaacaatat gcaggtacag aagttaaacg
     6301 agataatgag acttatttag ttttaaatga agaagatatt ttagcggtaa ttgaataata
     6361 tagaatataa ttcatagata gattgtaaag aacgaaaatg aaatatgact aaacaaatgg
     6421 aggtttatca tttatggtta aacaattgaa attctctgaa gatgcacgtc aagcaatgtt
     6481 acgtggtgtt gaccaattag caaatgcagt taaagtaacg attggtccta aaggacgtaa
     6541 tgttgtatta gataaagagt ttacagcacc tttaattacg aacgatggtg taacgattgc
     6601 taaagaaatc gaattagaag atccatatga aaatatgggg gctaaactag ttcaagaagt
     6661 cgcaaataag acaaatgaaa ttgctggtga cggtacgaca actgcaacag tattagctca
     6721 agcaatgatt caagaaggct tgaaaaatgt tacaagtggt gcgaacccag ttggtttacg
     6781 acaaggtatc gacaaagcag ttaaagttgc tgttgaagcg ttacatgaaa attctcaaaa
     6841 agttgaaaat aaaaatgaaa ttgcgcaagt aggtgcgatt tcagcagcag atgaagaaat
     6901 tggacgctat atttcagaag ctatggaaaa agtaggtaac gatggtgtca ttacaattga
     6961 agaatcaaat ggcttgaaca ctgaacttga agtggttgaa ggtatgcaat ttgatcgtgg
     7021 ttatcaatca ccgtatatgg ttactgattc agataaaatg gttgctgaat tagaacgccc
     7081 atatatttta gtaacagata aaaaaatctc ttctttccaa gatatcttac ctttattaga
     7141 acaagtggtt caatctaatc gtccaatctt aattgtagct gatgaagttg aaggcgatgc
     7201 attaacaaat atcgtactaa accgcatgcg tggtacattt acagctgttg ctgtaaaagc
     7261 acctggtttt ggtgatcgta gaaaagcgat gcttgaagat ttagctattt taactggtgc
     7321 gcaagtgatt actgatgatt taggcttaga tttaaaagac gcatcaatag atatgttagg
     7381 tactgcaagt aaagtagaag taactaaaga taatacaact gttgttgatg gtgacggtga
     7441 cgaaaacagc attgatgcac gcgtaagcca attgaaatct caaattgaag aaactgaatc
     7501 tgactttgat cgtgaaaaat tacaagagcg cttagctaaa ttagcaggcg gtgttgcagt
     7561 tatcaaggta ggtgcagcaa gtgaaacaga acttaaagaa cgtaaattac gtattgaaga
     7621 tgctttaaac tctacacgtg cagcagttga agaaggtatt gttgcaggtg gtggtactgc
     7681 actagtaaat gtttaccaaa aagtaagtga aattgaagct gaaggcgaca ttgaaacagg
     7741 tgtgaatatt gtacttaaag cattaactgc accagttcgc caaattgctg aaaatgcagg
     7801 gttagaaggc tctgtcatcg ttgaacgctt gaaaaatgca gaaccaggtg ttggttttaa
     7861 cgctgctaca aacgagtggg ttaatatgtt agaagcaggt atcgttgatc caactaaagt
     7921 aacacgctca gcattacaac atgcagcaag tgttgcagca atgttcttaa ctactgaagc
     7981 agtagtggct tcaattcctg aaaaaaataa tgaccaacct aacatgggtg gcatgtcagg
     8041 aatgatgtaa aactgtttat agttttgact aactagaaca ttccatgata gacgagtgtg
     8101 attcgttcta aataaatatt agagcgtatg aaaatatgtt tttaagacgt tttccattag
     8161 ttaccaaaac ttttggaagg cgtctttatt atgtgctgat gtgttattta gattcataat
     8221 gggggttttt tcaatgttaa aatcatatag attatataga gaagtttagt ataccttttg
     8281 agtatataag gagtttcttc tagtatgttg taatgtaatt agatttccgg taatcaattc
     8341 ggctttgcag aggactcact tgcgtattgt agtaagaagc tgtctgcatt ttgaaaacac
     8401 ccacactagt tacagcatgg gtgtttttat gctaagaatc aaattggttt aagtgcaaaa
     8461 tcatatacac taaagaaatt tattgaatct attgtcgaaa agatgctttc atgatacatt
     8521 gtaaacaaga agacaacgta gacaatgtat acttggttgt caaatgaagt aaaataagga
     8581 agtgagtagt atggctacaa taagtattac tactgattat aaattcacaa aaaaatcagc
     8641 gcaaaaacta atagatgcaa tggagattaa cgaaaataat agtaatgtga acaaaacaaa
     8701 cataaaagcg actaaaataa aatcaacttg tgaggttgaa aatctattga aggactatcg
     8761 aagtaattga ctgtaaaagt aatatcactt tcagaattgt taacaggtga taagcaagag
     8821 gttaagcgaa agataccttc agttttaaat atagtaaatt catttgagat aatatcaatt
     8881 tcaggaagtg aatcagcgca cgatgttgat ttatttttga aaaataagtc tatagcattt
     8941 gatagacaaa acctgtctag aactcattta gttttttcac aattcaaaag caaacaaata
     9001 ctagttggct attttacaat tagcaataaa cccttagttt ttacaaaacg tatgttagat
     9061 aaaatatcaa acacgttaaa gaagaagtta tatcaaaagg gtgaaactca cagtggaaat
     9121 gacaatttaa tcatacaagg atacttaatt gctcaaatag gaaaaaatta ttctgaagaa
     9181 gcattggcta caaaatccat aaatggaaac gatttgttaa ctttagcata tgagaaagtt
     9241 ttagaaggtg cgagcatatt tggcggttca tatatttgga tagagtatga agatgttgat
     9301 agattaagag aattttatag aaaatttgga tttacagaaa tcaaagatca tacaagtgaa
     9361 aataatttga agatggctat tcttaaaata taaggcacaa aaccacaccc acctattgat
     9421 ttaggagtgt ggttattttt atggaagaat ttataaaata aaggtcaaag acatttgaat
     9481 atttatcaac tgctcttcta tcaacaccca tgtgtctagc tatttcactt ttgcttattt
     9541 tcatgtttaa gttcatcata acaattattc agttttgtaa atatgaaaga gtagtaactt
     9601 caaaatccgt atttatctct agaattttag aaaatgtaca tgtttaaaca atcaaaagta
     9661 tacattatta aattatcatt tccattcatc ttttcaacaa ttgaggttaa cgtagtaaaa
     9721 aataagtttt aaatatttaa atcattgtat agtttagttt tgagtagatc tttttaaagg
     9781 tgtacttatg catttgcttt tttaaagaga taagacattt aatgggccta aaataaatgc
     9841 agaataaata aaaaactacc tgtttaggta gttttttaaa tgtaaaagat taaaacacta
     9901 gttcatttct tattaaagat tgatagctac tttatagata aatttgtcct ttagtgtagc
     9961 ggtaattttt tggacttttt ggtggtataa atgttcttaa taaagttaat agtcctactt
    10021 taccgcaaag cataacgaat ataataatta ttttagtaat accatgatat tctgtggtaa
    10081 ggttcatact taacccgact gttccaaatg cagaaaccac ttcgaataat aacttgatta
    10141 atgatatgtt cggattaatt atcgataat
//
LOCUS       DAPIDM010000091        10047 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000091 DAPIDM010000000
VERSION     DAPIDM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.091"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            140..1105
                     /locus_tag="RJE42_001882"
     CDS             140..1105
                     /locus_tag="RJE42_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV5877296.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEVDSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            1441..2517
                     /locus_tag="RJE42_001883"
     CDS             1441..2517
                     /locus_tag="RJE42_001883"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDV5877297.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDLERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            2763..3446
                     /locus_tag="RJE42_001884"
     CDS             2763..3446
                     /locus_tag="RJE42_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDV5877298.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADANEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(3562..4224)
                     /locus_tag="RJE42_001885"
     CDS             complement(3562..4224)
                     /locus_tag="RJE42_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDV5877299.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPDSLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(4217..4834)
                     /locus_tag="RJE42_001886"
     CDS             complement(4217..4834)
                     /locus_tag="RJE42_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDV5877300.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSIEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            4901..5371
                     /locus_tag="RJE42_001887"
     CDS             4901..5371
                     /locus_tag="RJE42_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDV5877301.1"
                     /translation="MTNDIYFMTLAIEEAKKAARLGEVPIGAVITKGDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            5518..6387
                     /locus_tag="RJE42_001888"
     CDS             5518..6387
                     /locus_tag="RJE42_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDV5877302.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     KNIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDVSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            6407..6973
                     /locus_tag="RJE42_001889"
     CDS             6407..6973
                     /locus_tag="RJE42_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDV5877303.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPAIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSAEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFAHNSPYEHLK"
     gene            7406..9802
                     /locus_tag="RJE42_001890"
     CDS             7406..9802
                     /locus_tag="RJE42_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HDV5877304.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTVPTENKTTEKVDSRQQNNIEQSTTSNQPKVNESDNT
                     SVKETTEEPQNTTSTQSTKQNNDATANKDNLEAQNISTQAKDVSATPKTTTIKPRTLN
                     RMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNSTTGKTEFWATSSDVLKLKANYT
                     IDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTYTFT
                     NYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDAYSEEIIVDYGNKKAQPL
                     ISSTNYINNEDLSRNMTVYVNQPKNTYTKETFVSTLTGYKFNPDAKNFKIYEVTDQNQ
                     FVDSFTPDTSKLKEVTNQFNITYSNDNKTATVDLLNGQTSSNKQYIIQQVAYPDNTST
                     DNGKIDYTLETQNGKSSWSNSYSSVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANE
                     KGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLGNGTYSVEFSTPAGYTPTTANAGT
                     DDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKG
                     VKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAG
                     KHTPVKPMSATKDHHNNAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK
                     "
BASE COUNT         3787 a         1390 c         1789 g         3081 t
ORIGIN      
        1 ccatttacag aaacatcagc aacatgtaca tgtcatatat acaagaaagc gcttttatat
       61 tataattatt atgaaaatga atattatgtc cgcatgtggt ggactatggt tatatagaag
      121 agacaaagga gatatttcta tgaaaaaaat tatgattact ggtgcattag gacaaattgg
      181 tacagaatta gttgttaagt gcagagaaat ttatgggaca gataatgttc ttgctacaga
      241 tattagggaa cctgaagtag actcacctgt acaaaatgga ccatttgaaa tcttagacgt
      301 aacagatcgt gaccgcatgt ttgagttagt tagggacttt gaagcggata gtctaatgca
      361 tatggcagca ttattatcag caactgctga gaaaaatcca attcttgctt gggatttaaa
      421 tatgggtgga ttaatgaatg cattagaagc tgcaagaact tataatttgc actttttcac
      481 accaagttca attggtgcat ttggagactc aactcctaaa gttaatacgc cacaagtaac
      541 aattcagcaa cctacgacaa tgtatggtgt aaataaagta gctggagaat tattgtgtca
      601 atactatttc aaacgttttg gtgtagatac aagaagtgtt agattcccag gtttaatctc
      661 gcatgttaaa gagccaggtg gcggtactac agactatgcg gttgaaatat acttcaaagc
      721 agtaagagaa ggtcattata caagcttcat agataaaggc acgtatatgg atatgatgta
      781 tatggatgat gcaattgaag caattattaa acttatggaa gcagacgacg ctaaattaga
      841 aactagaaat ggttataatt tgagcgcaat gagttttgat ccagagatgg taaaagaagc
      901 aattcaagaa tactatccaa atttcacatt agattacgat gttgatccta ttagacaagg
      961 tatcgctaat agttggccgg attctattga tacaagctgt tcacgtggcg aatggggatt
     1021 tgatcctaaa tatgatttag cgagcatgac taaattaatg ttagaagcta ttgaacaaaa
     1081 agatactgtt aaaaataata actaatcatt tccattcact ttaatacacg gaatgatatt
     1141 ttaaattact ctttatttta ataaactagt gcatgaattc taatattatt cattatacat
     1201 attgaattcg cgagcttagt tcattttaaa gtaaagagtt ttttgtatgt aaagtatatt
     1261 agtaaaacac agattacttg tctctatgaa aataatacgt ttatactaaa agatatagtc
     1321 tttctagaaa atctgttaat taattttgaa tttttagaaa atttattgaa cagcaaaata
     1381 tggattgtta taatttaagt taaacaaatt cttatacaat attattagga ggcaatcacc
     1441 atgtcacaag cagttaaagt tgaacgacga gaaacattaa aacaaaaacc aaatacatct
     1501 caactaggtt ttggtaaata ttttactgat tatatgttga gttatgatta tgatgcagat
     1561 aaaggatggc atgatttgaa gatagtacct tatggtccta ttgaaatttc acctgctgca
     1621 caaggtgttc attatggtca atcggtattc gaaggattaa aagcatataa aagagatggg
     1681 gaagttgcac ttttccgtcc tgaagaaaat tttaagcgtc ttaataactc gttagcacgc
     1741 ttagaaatgc ctcaagtaga cgaagcagaa ttgttagagg ggctaaaaca attagttgat
     1801 ttagaaagag attggattcc tgaaggggaa ggtcaatcat tatatattcg tccatttgtt
     1861 tttgcgacag aaggggcact tggcgttggt gcatcacatc agtataaatt attaattatt
     1921 ttatctcctt caggtgcata ttatggtggt gaaactttaa aaccaactaa aatctatgta
     1981 gaagatgaat atgtgcgtgc tgttcgtggc ggtgtaggct ttgcgaaagt tgcaggtaac
     2041 tatgcggcaa gtttattagc acaaactaat gcaaataaat taggttatga ccaagtacta
     2101 tggcttgatg gtgttgaaca gaaatatatc gaagaagttg gtagcatgaa cattttcttc
     2161 gttgaaaatg gcaaagtaat tacaccagag ttgaatggca gtattttacc tggtattaca
     2221 cgtaaatcta ttatcgaatt agctaaaaac ttagggtatg aagtcgaaga gcgtcgcgtt
     2281 tcaatcgatg aattattcga atcatatgat aaaggtgagt taacagaagt atttggtagt
     2341 ggtactgcag cagttatttc acctgtaggt acattgagat atgaagatcg tgaaatcgtt
     2401 attaataata atgagactgg tgaaattact caaaaattat acgacgtcta tactggtatt
     2461 caaaatggta ctttagaaga taaaaatggt tggagagtcg ttgtaccaaa atattaataa
     2521 aaattgaata tgatcatgaa aacaatatgt aaaagttaat catgactaaa tccctttcaa
     2581 tgaagcgaag catggtaata aattaagttt acaatgttta ttgttaagta ttgaagggga
     2641 tttcacttat tattatattt aattcaatat ttaaaaagaa ctatactacg agttcatttt
     2701 caggggaata acgaacatca tagaatcagc ataatacgaa ataaatagga gtattgatat
     2761 taatggaatg gatattattt gataaagatg gtacgttaat tgaatttgat agaagttggg
     2821 aaaaaatagg ggtacgattt gtacaatcat tgcttgagac tttcccagta cataataaag
     2881 aagctgcttt aagacaactc ggtgtcatta aagaatctat tgatccaaaa tcagtgatgg
     2941 gttcaggatc tttacaacaa attattcagg catttaatga tgtgacggga caagatacaa
     3001 ccgactggtc caagtcaaca agtcaaaagt tggtagatga acgtattcct gaaattaatt
     3061 gggtagaagg tgttaaagaa gcacttatcg atttgaaagc aaaaggctat caactaggta
     3121 ttgttacgag tgatactaaa aaaggtgtag aacaattttt agcacatacc aatgctacct
     3181 cattgttcga tttgatcatt tctaccgaag cggatgccaa tgagaagcca aatcctaaag
     3241 tattatcgcc tttatttgag caatataatg tagacccgca gaaagtagct atagtaggag
     3301 acactgctaa tgatatgaag acagcaagta atgcaaattt aggtatggca ataggtgtat
     3361 taacaggtat tgcaacaaaa gaagaattac atgaagctga tattatttta aatagtgcgg
     3421 cagatatttt agaagcttta aattaaaaag taagacatag tgtatatgtg aatcaatgta
     3481 accaatagaa ttgataatgt aatcgatgat atctatggaa tatacattga atataaacat
     3541 atacgctatg tctttagtct tttatcgtgt atctacttgt cgatatgttt gaataattcg
     3601 agcgatttta tcaatcatag gatttaaaga atcaggatct ttgtgtatat catattcatt
     3661 aatgttgata cgtaccacag gacatgcatt aaagctgtta atccaatcgt cataacgttt
     3721 aaataacttt ttccaatatt caggatcagt atttatttcc atttcgcgac cacgttcaat
     3781 aatacgatca atgacctcat cataattaca ttctaaataa atcattacat caggtttagg
     3841 aaaataaggt gtcatgacca tggcattaaa taagtctgaa tatgttttga aatcctcttt
     3901 actcattgtg ccttcttctt catgcatttt tgcaaaaata tcaacatctt cataaattga
     3961 tcgatcttgg acaaagccac caccatattc aaacatacgc ttttgttctt taaagcgttc
     4021 agctaagaaa taaatttgta aatggaaact ccatcgttca aaatcgctgt aaaatttatc
     4081 taaatatgga ttatgttcga cattttcaaa agacgtttta aagtttaatt tatctgcaag
     4141 tgcttgcgtt agtgttgatt ttccaacacc aactgtacct gcaatggtta taatggcatt
     4201 ttgtggaata ccgtaattat tcattggtaa tatctcctat cataggtaat ataatatgta
     4261 atatatcttc gtaatcttgt tcatttttaa gaaaatcaat agaagttgta tcgattaaaa
     4321 ctacatttga accattactt tgtaaggact cataatactc acgataatct ttttttaact
     4381 ttaacagata ttcatcttct atttgatgct caaaactacg gttacgttta gcaattctag
     4441 attttaacac atcaaggtct gcatctaaaa agataatcat attcggcata atcatatctt
     4501 cagttaaaat atcataaatt ttactaaatt tctgaaattc aatagaactc aaggtatttt
     4561 tagcaaatat cttattttta tgtatatgat aatcactaac tacaccttga tttagttgtg
     4621 ttacatcttg aaattgctta tatctattgc ataaaaagaa catttcagtt tgaaaactcc
     4681 atttagagat atcttcataa aagtctgata aaaatggatt ttctgtgatg atttcttttt
     4741 cttcataaaa atctaaagtt tgacttaatt tgtgtgcaag tgaagattta cctacgccaa
     4801 taggaccttc aattgctata aaaggtttgt tcatattcac atctccaaat gttaaaatag
     4861 acattgagta ttgtaccata agtgaggtat ctggacataa atgacaaatg atatatattt
     4921 tatgacatta gcgattgaag aagctaaaaa agctgctcgg ctaggcgaag tacctatagg
     4981 tgccgtcatc actaaaggtg atgaagttat cgctagagca cataatttaa gagaaacact
     5041 acaacaacca acggcgcatg ctgaacatat tgcaattgaa cgtgcagcca aagtgttagg
     5101 tagttggcgt ttagaaggtt gcacattata tgtaacctta gaaccatgtg ttatgtgcgc
     5161 aggaacaatt gtaatgagtc gcattccaag agtcgtctat ggcgcagatg atcctaaagg
     5221 tggttgtagt ggcagtttaa tgaatttatt gcaacaatct aattttaatc atcgtgcaat
     5281 tgttgataaa ggtgtactta aagaagcatg tagcacatta ttaacaacat tttttaaaaa
     5341 cttaagagcc aataagaaat ccaccaatta gacgatagta catactaatt aaaatttgtt
     5401 agaattacat tatatgataa attaatgaca atataaatgt taatagaaaa gtatgtgcgc
     5461 tgagtatata ttcttattta agaacaagat tttaaattta cgaaacgagg taacataatg
     5521 ataaaactaa tagccactga tatggatggc acgctactta atgcagcaca tgaaatttct
     5581 caacctaata ttgatgcgat taaatacgct caagaacaag ggataacggt tgttatcgcg
     5641 acaggtcgag cattttatga agcacaagca ccagttgctg acacagattt aacagtacca
     5701 tatatttgtt tgaatggtgc tgaagtacgt gatgaaactt tcaatgtaat gagcacttca
     5761 caccttaata aatcgttagt acacaaaatt acaaatgttt taaaagatgc aggtatttat
     5821 tatcaagtat acacgagtcg tgcgatttat actgaagatc cacaaagaga tttagacatt
     5881 tacatagata ttgctgagcg tgcaggtcaa cacgcaaacg ttgagcgtat taaaaatggt
     5941 attcaaagac gcatagataa tggtacgttg aaagttgttg ataattatga tgctattaaa
     6001 aacattcctg gtgaattaat tatgaaaata ttagcatttg atggaaattt agaaaaaatt
     6061 gacaaagcta gtaaaatttt agctgaatct ccgaatttag ctatatcatc atcttcgaga
     6121 ggaaatatag aaataacgca ttcagatgca caaaaaggta ttgcgctaga aacaattgcc
     6181 gaaagattag ggattgaaat gaaagatgtc atggcaatag gtgacaattt aaatgacgta
     6241 tcaatgttag agaaagttgg ctatccagtt gcgatggaaa atggtgctga agaagttaaa
     6301 aaaatagcaa aatatgtcac agatacgaat gaaaatagtg gtgttggaaa agctattatg
     6361 aaattattac gtgaacaaca agtttaataa aaaagagggg tcaaatatga aaggattaat
     6421 tattattggc agtgcacaag tgaattcaca tactagtgca ctagcaagat acttaactga
     6481 gcattttaaa acacatgata tagaagcgga aatattcgat ttagcagaaa aaccattaaa
     6541 tcaattagat ttttctggta caacgccggc tattgatgaa atcaaacaaa atatgaaaga
     6601 tttaaaagag aaagcaatgg cggcggactt tttaatatta ggaacgccaa actatcatgg
     6661 ttcatattct ggaatattga aaaatgcatt agatcatcta aatatggatt attttaaaat
     6721 gaagccagta ggattaatag gcaatagtgg aggtattgtc agcgctgaac cgttatcaca
     6781 tttaagagtt attgtcagaa gtttattagg cattgcagta ccaactcaaa ttgcaacaca
     6841 tgattcagat tttgctaaaa atgaagatgg ttcatattac ttaaatgaca gtgaattcca
     6901 attacgtgca agattatttg tcgatcaaat tgtatcattt gcgcataata gtccatatga
     6961 acatttaaaa taaattaaaa aacatgtaaa tatacattga aaataggtat gtagcttata
     7021 aagcaaaaca attgctttga agttacatac cttttttatt gattttgtgt ttaattaagc
     7081 ttcgatttag aatttggata tttagcgtaa ctaaaagatg atttatcgaa atccgattta
     7141 aacatatatt taagtttttt gtgaatagta tgagcgaaca atgttgtcta taaattttag
     7201 actataaaag aaaaagtata ttaaaaattg ctatctttaa tatgtatcat gtaaaaatta
     7261 attagtaaaa taacattgta aaataaaaga aaaaaattaa aacattgtac tttatcgaaa
     7321 agtgtattaa gatagaaagg cagaaatttt tatatgcaaa atattatatt tatcaaattt
     7381 tgatatttta aaggagtatt attaaatgaa taataaaaag acagcaacaa atagaaaagg
     7441 catgatacca aatcgattaa acaaattttc gataagaaag tattctgtag gtactgcttc
     7501 aattttagtg gggacaacat tgatttttgg gttaagtggt catgaagcta aagcggcaga
     7561 acatacgaat ggagaattaa atcaatcaaa aaatgaaacg acagttccaa ctgagaataa
     7621 aacaactgaa aaagttgata gccgtcaaca aaataatatt gaacaaagta caacgtcaaa
     7681 ccaaccaaaa gtaaatgagt ctgataatac ttcagttaaa gaaactactg aagaacctca
     7741 aaatacaact tctacacaat caactaaaca aaataacgat gcgacggcaa ataaagataa
     7801 tttagaggct caaaatattt caacgcaggc taaagatgtt tcagctactc ccaaaacaac
     7861 gactattaaa ccaagaacat taaaccgtat ggctgtgaac acagtagcag caccacaaca
     7921 aggtacaaat gttaatgata aagttcattt ttcaaatatt gatattgcta ttgataaagg
     7981 acatgtcaat tctacaacag gtaaaactga attttgggcg acttctagtg atgttttaaa
     8041 attaaaagcg aattacacaa tcgatgattc tgttaaagag ggcgatacat ttacatttaa
     8101 atatggacag tatttccgtc cgggttctgt aagattacct tcacaaactc aaaatttata
     8161 taatgcccaa ggtaatatta ttgcaaaagg tatttatgat agtacaacaa acacaacaac
     8221 atatactttt acgaactatg tggatcaata tacaaatgtt agcggtagct ttgaacaagt
     8281 tgcatttgcg aaacgtgaaa atgcaacaac tgataaaact gcttataaaa tggaagtaac
     8341 tttaggtaat gatgcgtata gcgaagaaat catcgtcgat tatggtaata aaaaagcaca
     8401 accgcttatt tcaagtacaa actatatcaa taatgaagat ttatcacgaa atatgactgt
     8461 ttatgtaaat caacctaaaa atacatacac aaaagaaaca tttgttagta ctttaacagg
     8521 ttataaattt aatccagatg caaaaaactt taaaatttat gaagtgacag atcaaaatca
     8581 atttgttgat agctttacac ctgacacttc aaaacttaaa gaggtaacta atcaatttaa
     8641 tattacttac agcaatgata ataagactgc aactgtagat ttattgaatg gccaaacaag
     8701 cagtaataaa caatacatca ttcaacaagt tgcttatcca gataatacat caacagataa
     8761 tggaaaaatt gattatacat tagaaacaca aaatggtaag agtagttggt cgaacagtta
     8821 ttcaagtgta aacggctcat caactgctaa tggcgaccaa aagaaatata atctaggtga
     8881 ctatgtttgg gaagatacaa ataaagatgg taaacaagat gccaatgaaa aagggattaa
     8941 aggtgtttat gtcattctta aagatagtaa cggtaaagaa ttagatcgta ctactactga
     9001 tgaaaatggt aaatatcagt ttacaggatt gggaaatggg acttacagtg tagaattttc
     9061 aacgccagca gggtacacac caactactgc aaatgcagga actgatgatg ctgtagattc
     9121 tgatggacta actacaacag gtgtcattaa agacgctgac aacatgacat tagatagtgg
     9181 attctacaaa acgccaaaat atagtttagg tgattatgtt tggtacgaca gtaataaaga
     9241 tggtaaacaa gattcgactg aaaaaggaat taaaggtgtt aaagttactt tgcaaaatga
     9301 aaaaggcgaa gtaattggta caactgaaac agatgaaaat ggtaaatatc gttttgataa
     9361 tttagatagt ggtaaataca aagttatctt tgaaaagcct gctggcttaa ctcaaacagt
     9421 tacaaataca actgaagatg ataaagatgc agatggtggc gaagtagacg taacaattac
     9481 ggatcatgat gatttcacac ttgataacgg atacttcgaa gaagaaacat cagacagcga
     9541 ctcagattca gacagcgact cagattcaga tagcgactca gactcagaca gtgactcaga
     9601 ttcagacagt gactcagatg caggaaaaca tacacctgtt aaaccaatga gtgctactaa
     9661 agaccatcac aataatgcaa aagcattacc agaaacaggt agtgaaaata acggctcaaa
     9721 taacgcaacg ttatttggtg gattattcgc ggcattagga tcattattgt tattcggtcg
     9781 tcgtaaaaaa caaaataaat aatatgaata actaagccag gtctttgtga cctggttttg
     9841 tatttataaa tacgaaaggt ataaaacaaa agttgtaatt cttaaataca atttaattta
     9901 atgaaaaaag taagtgaaaa tgaattagaa aatcttaggt gtttattttg ggtttggata
     9961 atactatttt tatgataatt aaataaaatt tacaaaactc ataaaaaatc ttatttaaac
    10021 gcaaatgtta aataacgttt tacgata
//
LOCUS       DAPIDM010000092        10030 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000092 DAPIDM010000000
VERSION     DAPIDM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.092"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            77..472
                     /locus_tag="RJE42_001891"
     CDS             77..472
                     /locus_tag="RJE42_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877305.1"
                     /translation="MVVSTGSYILSELPCIVAYKNNDIVGLLTYKVYDEYIEIISLDS
                     FVENKGIGSHLLNYAEIIASDMSKRSISVITTNENIKALYFYQKNKYRITDVIFDAVT
                     EARKIKPSIPEMGENGIEIRDEIVLRKCL"
     gene            complement(580..1566)
                     /locus_tag="RJE42_001892"
     CDS             complement(580..1566)
                     /locus_tag="RJE42_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDV5877306.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDAKIEL"
     gene            1875..2078
                     /locus_tag="RJE42_001893"
     CDS             1875..2078
                     /locus_tag="RJE42_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877307.1"
                     /translation="MEDLKHSLKGLGWWDLFFAIPMFLLFVYLPNYNFITIFINIVII
                     IFFSIGLILTTHIIIDKIKSNSK"
     gene            2127..2423
                     /locus_tag="RJE42_001894"
     CDS             2127..2423
                     /locus_tag="RJE42_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDV5877308.1"
                     /translation="MATEKDVNDLFLNHVNSNAVKTRKMMGEYIVYYDGVVIGGLYDN
                     RLLVKATKSARHQLQDNTLISPYPGAKEMILIPDITEATNLTNLFECIKNDLKK"
     gene            complement(2486..3706)
                     /locus_tag="RJE42_001895"
     CDS             complement(2486..3706)
                     /locus_tag="RJE42_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5877309.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YEINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(3699..4250)
                     /locus_tag="RJE42_001896"
     CDS             complement(3699..4250)
                     /locus_tag="RJE42_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDV5877310.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLNVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPYDIL
                     NDSQFIKELANINLALKKGDIYD"
     gene            4363..5223
                     /locus_tag="RJE42_001897"
     CDS             4363..5223
                     /locus_tag="RJE42_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV5877311.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLEKQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKDMKDFKLNDIQNHTLLVTEKGCSYREQLEKILYKYSFSTKQMIEFL
                     GIESLKKHLKNSDGIALLPEFIVADELQNKSLFQIDIDVDIPNLETTLIINPESSKQV
                     LESFVKDVFL"
     gene            5847..6449
                     /locus_tag="RJE42_001898"
     CDS             5847..6449
                     /locus_tag="RJE42_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877312.1"
                     /translation="MLISLVIPVFALLVIGGIIWAIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVIFTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            complement(6741..7655)
                     /locus_tag="RJE42_001899"
     CDS             complement(6741..7655)
                     /locus_tag="RJE42_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877313.1"
                     /translation="MKQSVNFKSKTNLLAANLYTPEQFDESKKYPAITVCHPGGGVKE
                     QTAGMYAEKLAEFGYVTVVYDASHQGESEGSPRYLEDPFARTEDVRASVDYLTTLDFV
                     DNDRIGALGVCAGGGYTVSAARTERRIKALATVSMVDIGALFRKGPGDVVSIDDQLAF
                     MTQVADQRTAEANGADYGITGYVPEEIDDSMPENSTMVQGHDYYLTARGQHKNAPNKL
                     LLRSFSEVLTFAPFTYIDELLTQPFLAIAGTEADTIEYSVDAVEKAAGDNNELYKIED
                     ASHVDLYDIPEYVNQVLPKLESFYKETL"
     gene            7834..8388
                     /locus_tag="RJE42_001900"
     CDS             7834..8388
                     /locus_tag="RJE42_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDV5877314.1"
                     /translation="MNVGDLRVVKTRASIKKAFMTLLFEKDFDTISIKEITEFAQIGR
                     KTFYLHYIDKYDLLDQIVSEKLIELEQISEAKKSLGIQEGTQLWFEFFENNRTFFMKI
                     FNINNASNYKKKLLHFIEEEFKKKVPTRVATEKGLDYDLYINFISNGIIGLLDIYLNS
                     SENTEEQEKITLQVSRLLSLYDLI"
     gene            8619..9350
                     /locus_tag="RJE42_001901"
     CDS             8619..9350
                     /locus_tag="RJE42_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV5877315.1"
                     /translation="MQNRNDFIEKLLDRAQIKEGMRVLDIGCATGEVTQLIAKRVGAN
                     GEVVGVDVNESLLKIANENNQYNNVSYQYSDIYQLPETMGHFDAIVGRRVLMYLPDAE
                     KCLQILKSILKPEGILCFQESDAINAGVGADALSLHQSAIQWIWETVKQEGGNIHIGQ
                     NLYNLLHNNGMHVVDYFAEAVIQTSMDNDLAWLVDVMLPRMKAHGVINDDFSLDEFKL
                     NLEHEAMNNQCAFIRDMAFGIIGKA"
     gene            9505..9987
                     /locus_tag="RJE42_001902"
     CDS             9505..9987
                     /locus_tag="RJE42_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877316.1"
                     /translation="MKKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIESLIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKAILLAILFTSLALTAGTVADIYGITDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         3522 a         1507 c         1632 g         3369 t
ORIGIN      
        1 ggaatataaa tcttacaaca aaatgagtaa taatatttta tctatcttgt cgagtgaaaa
       61 aaatttcgat aataaaatgg tagtctcaac aggtagttac attttgtctg agttgccatg
      121 tattgttgcc tataaaaata atgatatcgt ggggctacta acgtataaag tttatgatga
      181 gtatattgaa atcatttctt tagatagttt tgtagagaac aagggtattg gtagtcattt
      241 acttaattat gctgaaataa ttgcctcaga tatgagtaaa agaagtatca gtgttattac
      301 tacaaatgaa aatattaagg cactctattt ctatcaaaaa aataaatata gaatcacaga
      361 cgttattttt gatgctgtaa cagaagctag gaaaataaag ccatcgattc ctgaaatggg
      421 tgaaaatggc attgaaatta gagatgaaat tgttttgagg aaatgtttgt aatgtataaa
      481 tatactcagc tttaaatcac cctagttcat accaaaaagt ctttaacaca aacaaaaaag
      541 gaggaaactt aaattcctcc tcaaccttta ttactcatac tataattcaa ttttagcgtc
      601 ttcgtccatt tgggcttcaa attcatctag tagtgctcgt gcttctgcaa ttgattgtgt
      661 gttcatcaat tgatggcgaa gttcactagc gcctcttatg ccacgcacat agattttaaa
      721 gaatctacgc aagctcttga attgtcgtat ttcatctttt tcatatttgt taaacaatga
      781 taaatgcaat ctcaacagat ctaatagttc cttgcttgtg tgttcgcgtg gttctttttc
      841 aaaagcgaat ggattatgga aaatgcctct accaatcatg acgccatcaa tgccatattt
      901 ttctgcaagc tcaagtcctg tttttctatc gggaatatca ccgttaattg ttaacaatgt
      961 atttggtgca atttcgtcac gtaaattttt aatagcttcg attaattccc aatgtgcatc
     1021 tactttactc atttctttac gtgtacgaag atgaatagat aaatttgcaa tgtcttgttc
     1081 aaaaacgtgc ttcaaccaat ctttccattc atcgatttca tagtagccaa ggcgtgtttt
     1141 aacacttacc ggaagcccac ctgctttagt tgcttgaata atttcggcag cgacgtcagg
     1201 tcttaagatt aagccggaac ccttaccctt tttagcaaca tttgctacag gacatcccat
     1261 atttaagtct atgcctttaa agcccatttt agctaattga atactcgttt cacggaactg
     1321 ttctggctta tctccccata tgtgagcgac catcggctgt tcatcttcgc taaaagttaa
     1381 gcgtccgcgc acactatgta tgccttcagg gtggcaaaag ctctcggtat ttgtaaattc
     1441 agtgaaaaac acatccggtc tagctgcttc acttacaacg tgtcgaaaga cgatatctgt
     1501 aacgtcttcc attggcgcca aaataaaaaa tggacgtggt aattcactcc aaaaattttc
     1561 tttcataata tatttatacc ctctttataa ttagtatctc gattttttat gcatgatgat
     1621 attaccacaa aagcctaact tatacaaaag gaatttcaat agatgcaacc atttgaaagg
     1681 gaagtctaag agtagtctaa aataaatgtt gtggtaagtt gatcaatata caaatcaagg
     1741 attatcgtat tagattgttc attattaatg atacactact tattaatatg attcagaatt
     1801 ttctttagct acatatacag taatgtgatt tatacgttat cttataacaa agacaaattt
     1861 ataaaggtga tattatggaa gatttaaaac attctttaaa aggtttaggt tggtgggatt
     1921 tattttttgc gatacctatg tttctactat tcgtgtatct gccgaattat aattttataa
     1981 ctatatttat taacattgtt atcattattt tcttttccat aggtttgatt ttaactacgc
     2041 atataattat agataaaatt aagagcaact cgaaatgatt cattaatacg aatgtgatta
     2101 aacataaaac tgaaggagcg attacaatgg cgactgagaa agatgtaaat gatttatttt
     2161 taaatcatgt gaattcaaat gctgttaaaa ctagaaagat gatgggagaa tatattgttt
     2221 attatgatgg tgtggttata ggtggtttgt atgataatag attattggtc aaggcgacta
     2281 aaagtgcacg tcatcaattg caagataata cattaatatc gccatatcca ggtgctaaag
     2341 aaatgatatt aattccagac attaccgaag caacaaatct cactaattta tttgaatgca
     2401 taaaaaatga tttgaaaaag tgaagttcga atggagagaa ttaagcacat cgacaaatga
     2461 tcgcatatta aaagaagagt gacggtcact cttctttatg tgcatatttt attttgtctg
     2521 ttttgttaac aagcagcagt gtaacaaata tgagtaagga taaaatgagt ataatataga
     2581 aaccgaattt atcattaatt tcattaatcc atcttcctaa aaatggagca atgaaacttt
     2641 gcagcaacaa tgaaattgac gtccatatcg taaatgagcg accgacatat ttatctgaaa
     2701 cagtgttcat tatagctgta ttcatataaa ttctgattga tgaaattgag tagcctagta
     2761 taaatgatcc tatgaataag taaaatgctg agtttatcca aataaatagt gctgaattta
     2821 tgactaatat gaaatataac aaaaatatca atactttagt tgagattttc ttcgaaagaa
     2881 tagctgaaat taaacctgca cataatcctc caatgccata taacatatct gaaaaaccaa
     2941 attgtacaga cgaaagtttt aaaacattat atacatatcc tggtaatgat atgttaaaga
     3001 tcattgtaaa caccattggt atgattgaaa taactccaaa aataaatatc atcatgttgt
     3061 cttttaaaaa tttccatcct aataaatatt cttgcaataa gctatttgtt gattcttcct
     3121 ctgaatgagt tggtttatct acatgcaatc taaataacat aaaaatgctg attagaaaca
     3181 tcattatagt catcgctata attagagtga atccatttat ttcatataat attcctgata
     3241 atccacctgc aataaacata cctgtttgca aactaatctc taatagagaa tttgcatctg
     3301 tatattgatc tggttttaat atctgtttaa ctaaacttct agatgttgcc atatacgtag
     3361 tccaacctat cccattaaca atcgcaaatc caatcactaa ataggtctca aagcctatca
     3421 ttacgagtgc tatgacaatg agtaaatata atattacctg aagaagatag gtgattaaaa
     3481 taatgtttcg tctattatat ttatcggcta gtccacctat aataggagaa gctaagaaac
     3541 cggataatac atttaaagct aacataatac ctaatagttg ggagtcgtta gttttgtcga
     3601 ttaaatacca attagcccct acagtactaa ttccaactcc gaatgctgaa ataatttcag
     3661 ctaaaaagaa caatctgaag tttttaattt tcaacaaatc agtcataaat atcacctttt
     3721 ttcaatgcaa gatttatatt tgcgagctct tttataaatt gagagtcgtt taaaatatcg
     3781 tatggtttat tgccttcgcc aatcaaaaat ctatagttag taatattcat aaactcaaat
     3841 attaatttga attggttgac taaaggttgt gctttggtat gcggattgtc gccacctatg
     3901 attaaaatca aatacttttt ttgtgacatg atttctttga agttgtctat ttgcgtatct
     3961 cgcaatgact cggtccatct atcaatgaag agttttaaag acgcactcat ggagtaccaa
     4021 tagagtggtg ttgaaaaaat aataatatct gattctaaaa ctttgattaa aatttgttcg
     4081 taatcatcat tatgaaagtt ggcacctttg ctatgacgat tatctgtaac cttttcaatg
     4141 ttactttgat ataaattcac aaagttgaca tttaaattct tcaatagatt ctctactgcg
     4201 atagctgaat tgccatcttt tctactactt ccaaataaac aagtaatcat agtcataact
     4261 cctttcgatt tacctttata ataatattta atatattatt attaaatcag aattcttaga
     4321 atgtagaatt cgataaaagt gaatcctaaa ggaggtacta ctttgaactt aaacatttta
     4381 aaatcatttt tagtaactag tgaaacaaag aaccttacta aagcatcaga attacttaac
     4441 tattcacagt caactgtatc tacacatatt gaaaaattag aaaagcaatt agatgttaaa
     4501 ttattttata gaaaaaaata tggtatggaa ctaacggaag aaggcttagc atatgttaaa
     4561 tacgctaaag tgattttaga tagtaatagc gaatacgaga gagaaataaa aggactttac
     4621 aataagaagg taaatataag tattaacatg caagaaagtc agtatttgta tcgctactat
     4681 aataagatta gtgaatggtt agctgaacac ccatatgtaa acttgaagtt taaatccgca
     4741 cattctaatt tctatattaa agaagaaatt gctaatttca aatcggatat tagccttatc
     4801 acagacgaaa agattattaa tagtaactta actgctattc ctataactga agaacgttta
     4861 atatttgtta ctaacaaaga tatgaaagat ttcaaattaa acgatattca aaaccataca
     4921 ttacttgtta ctgaaaaagg gtgtagttac agagaacagt tagaaaagat tctctataaa
     4981 tatagctttt caacaaaaca aatgattgaa tttctaggaa ttgagtcatt gaaaaaacac
     5041 ttgaaaaatt cagatgggat tgcgctattg ccggagttta ttgttgcaga tgaattacaa
     5101 aataaaagtt tgtttcaaat agatatcgat gtcgatattc cgaatttaga aacaactttg
     5161 ataattaatc ctgaatccag taagcaagta ctagaatctt ttgtaaaaga tgtcttttta
     5221 taaatattgg tgaaaacttg tagttatggt gaaactcaaa gataatcatt tattggtaag
     5281 ggcatacgaa caattaaaat ttccaaacaa ggtacacgag tgcttctgaa tttatcggca
     5341 cgcttttaca tagatcaaaa gaacgcgcac taaaataatc attatatcta tgtaacttac
     5401 atctttgata ctacagaagt tcgtgcattt gttcaatcgt ctcataacca ttaaatgcca
     5461 ctaagcacga cctgactgtt tcacacttta tgaaaatctt taaatagcgt taattatgaa
     5521 tgaagacatc tataacattt atttatctta taaaatagta caattacaaa gcactacaac
     5581 ggcgactgga ctcaccttaa atcataacga caaccgctaa aaattgatcg caaaactaat
     5641 cattaattgc catacttaac tattttgaaa agtatcaatt tcacattata aaattattga
     5701 gtatctagat ttattgttat acacaaacag tatttttgtt atgatagagt taataaatat
     5761 aataaattga ataagtaact ttacaaaact agaatgattc aattcaatca taattgtttg
     5821 aaaatagagg tgagcaggtg aacgatatgt taattagtct cgtaatccca gtttttgctt
     5881 tgttagttat tggtggtatt atttgggcaa ttatagaagg tatagtacat atttcaaaaa
     5941 agaataaagc aattgataat ttttttaacc aagttaataa agtaagtgag acatataaat
     6001 tcgctactac ttttttattt ctaatcttgg ctacggctgg tatttctcaa ttttatctat
     6061 attatatagt atcagcgttt cttttttggc ttgtcatatt tacctttggt attgcaggta
     6121 tcattttttt aatgccatat ggcttatgct tcctaccact ttataagcaa aaaaagaaaa
     6181 aacagacatt taaaaaatac atggtttaca ctacgattgg tttgtcaatt tgtctaggct
     6241 tatctctagt tttagttcac actacgaaaa tttatatgga cgaaggtggc gtaagatact
     6301 attacggtag ttttgtaatg aaacaagcgg gcggttatgc ttatttagct ttagcggtac
     6361 tttcaacgct gttaattgtt gcgaaaaaag ctacaaataa aaataaagaa atcgaaaccg
     6421 tcgacaatac aaatataacg gaaagataat taagggagtg ctcattcagg agtgctcttt
     6481 tttgatgttc aaatttagtt ggtaatgaag aagcagaaac atttgtgagt ggaaaataaa
     6541 agttaagctg aaatcctatc gaacgtatct gtaaaacaaa aaattaacgc cacaattttc
     6601 atcgtaatta aaaacagaca ttctagtaac atgctttttc aaaaactaat ctaataatac
     6661 gtccatctat aaaatatttc tacgtactaa aaaaggcgga gtaaatcgtc cgcctcaatg
     6721 cacacaaccg catgttgttt ttatagagtt tctttataga atgattcaag ttttggtaac
     6781 acttgattta cgtattctgg aatatcatat aaatctacgt gtgatgcatc ttcaatttta
     6841 tagagttcgt tgttgtcgcc tgctgctttt tcaacagcgt caacgctata ttcaatcgtg
     6901 tctgcttctg tacctgcgat tgctaagaat ggttgtgtca acaattcgtc aatatatgtg
     6961 aaaggtgcaa atgttaatac ttctgaaaaa cttcttaata atagtttgtt tggtgcgttt
     7021 ttatgttgac cacgtgcagt taaataataa tcatgccctt gtaccattgt tgaattttca
     7081 ggcatagaat catcaatttc ttcaggcaca tagccagtga taccataatc tgcaccattt
     7141 gcttcagctg tacgttgatc tgcaacttgt gtcataaatg ccagttgatc atctattgat
     7201 actacatcgc caggaccttt acggaataaa gcgccaatat ctaccatact tacagttgca
     7261 agtgctttaa tgcgacgttc tgttcttgct gcacttactg tatatccacc acctgcacaa
     7321 acgccaagcg caccaattct gtcattatcc acaaagtcta atgttgtaag ataatcaact
     7381 gaagctctaa cgtcttctgt tctcgcaaat ggatcttcta aatatctagg agagccttca
     7441 ctttcacctt gatgtgatgc atcatataca accgtaacgt agccgaattc cgcaagtttc
     7501 tctgcataca tacctgcagt ttgttcttta acaccgccac ctgggtgaca aactgtaatt
     7561 gctggatatt ttttgctttc atcaaattgt tcaggtgtat ataaattcgc tgctaataaa
     7621 tttgttttac ttttaaaatt aactgattgt ttcatagatt aaatgcctcc atctttttat
     7681 ataagtcatt catttgttgt agatatttat tactcttgtg tgtataatgc catactagag
     7741 tatttaaatg tacaattcaa ttaacaagga agcacatatg tgttttaata cacagtttta
     7801 taaaaatggg ttttaaaaga aaggagtatt attatgaatg ttggggattt acgggttgtt
     7861 aaaacgaggg caagtattaa aaaggcattt atgaccttac tttttgaaaa agattttgat
     7921 actatttcga ttaaagaaat tactgaattt gcgcaaattg ggagaaaaac attttatcta
     7981 cattatattg ataaatatga tttactagat caaatagtat cggaaaaatt gatagaactt
     8041 gaacaaattt ctgaagccaa aaagagtctg ggtatccaag aaggcacaca gttatggttt
     8101 gagtttttcg aaaataatag aacatttttt atgaaaattt ttaatatcaa caacgcttca
     8161 aattataaga aaaagctgct tcattttatt gaagaagaat ttaagaaaaa agttccgaca
     8221 cgtgttgcta ctgaaaaagg cttagactac gacttatata ttaattttat tagtaacggt
     8281 attatcggac tactagatat ctatctaaac agcagtgaaa atacagaaga acaagaaaaa
     8341 ataacattgc aggtgtcgcg cctactatct ttatacgatt taatttaaaa ctaagctatc
     8401 tattttgaat ataattgcgt gtagcagata tagtatgtca cttcttgaga gaattttatt
     8461 ataatagttt tatgagttga tattgcagtg acaagagcta aaaaagaatt ttatgtaatt
     8521 ggcgacatgc aaagaataca gtcgaaacca ttttatgaga cgatttttaa aaaaatgtag
     8581 aataacatac aaaaaagtat atagagggag ttattttcat gcaaaataga aatgatttta
     8641 tagaaaagtt attagacaga gctcaaatta aagaaggcat gcgtgtactg gatataggtt
     8701 gtgcgactgg tgaggtcact caattaattg caaaacgtgt gggggcgaat ggtgaagtcg
     8761 tcggtgttga tgtgaatgaa tcattactta aaattgcaaa tgaaaacaat caatataaca
     8821 atgtatcata tcaatattct gatatatatc aattgccaga aactatgggg cattttgatg
     8881 cgattgttgg tagaagagta ttaatgtact taccagatgc tgaaaagtgt ttacaaattt
     8941 tgaaatcaat tttaaaacct gagggtatat tatgttttca agaaagtgat gctattaatg
     9001 caggtgttgg tgcagatgca ctatctttac atcaatcagc aattcaatgg atttgggaaa
     9061 cagtgaagca agaaggtggc aatattcata tcggtcaaaa tttatataac ttgcttcata
     9121 ataatggaat gcatgttgta gattattttg cagaggcagt gattcaaacg tcaatggaca
     9181 atgatttagc atggcttgta gatgtaatgc taccaagaat gaaagcgcat ggtgttatta
     9241 atgatgattt ttcactagat gagtttaaat taaaccttga acacgaagca atgaacaatc
     9301 agtgtgcatt tatacgtgac atggcgtttg gtattatcgg aaaagcctag tttaaaaacg
     9361 ctgaaagcag catattatca tcgccattgt tttaacttgt gaagttacta atggattttg
     9421 aaaataacag catttctatt ggctaatcaa gtgatactta attaccgagc aatttataca
     9481 ttttaaaagg agcaaaattg aataatgaag aaatttaaca actggatatt aaatgcaata
     9541 agtggatctc aaacagacaa gaatgaaaca actgaagaat taaaaggggc aaaatttatc
     9601 attttatatg catattcaat gctcgttttg cttgcgttag taatttctaa catattcatt
     9661 cacattttgg agcctaaact atcaatcacc actcaaatca tcatcgtttt gattttaatt
     9721 gaatcactaa ttggattgcg ttttttgaaa gcgtacgatg ttaagcgtgg caaagataaa
     9781 gaaaataaga aaaatagtaa ggatttcgtt aaactaaaag caattttact agcaatttta
     9841 tttacatcat tggcgctgac agcaggcact gtagctgata tatacggtat cactgactta
     9901 ggaaatacta ggagtgattt aatcgtttgg agtataggtg gtattatatt tggcctcgta
     9961 tgttacacaa tggaagataa aagataacga taaggagctg gcgattataa agctagctcc
    10021 ttttttaact
//
LOCUS       DAPIDM010000093         9937 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000093 DAPIDM010000000
VERSION     DAPIDM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.093"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(658..2657)
                     /locus_tag="RJE42_001903"
                     /pseudo
     CDS             complement(658..2657)
                     /locus_tag="RJE42_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2654..3409)
                     /locus_tag="RJE42_001904"
     CDS             complement(2654..3409)
                     /locus_tag="RJE42_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877317.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(3517..4404)
                     /gene="nsaS"
                     /locus_tag="RJE42_001905"
     CDS             complement(3517..4404)
                     /gene="nsaS"
                     /locus_tag="RJE42_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDV5877318.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(4415..5080)
                     /gene="nsaR"
                     /locus_tag="RJE42_001906"
     CDS             complement(4415..5080)
                     /gene="nsaR"
                     /locus_tag="RJE42_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDV5877319.1"
                     /translation="MKILIVEDDFVIAESLASELEKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSMTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(5106..5306)
                     /locus_tag="RJE42_001907"
     CDS             complement(5106..5306)
                     /locus_tag="RJE42_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877320.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTGSSNQEPSLQQAKEQKDDKG"
     gene            5504..6928
                     /locus_tag="RJE42_001908"
     CDS             5504..6928
                     /locus_tag="RJE42_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV5877321.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSAISKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVIATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFKFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6998..7108
                     /locus_tag="RJE42_001909"
     CDS             6998..7108
                     /locus_tag="RJE42_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877322.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(7211..7666)
                     /locus_tag="RJE42_001910"
     CDS             complement(7211..7666)
                     /locus_tag="RJE42_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV5877323.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(7910..8671)
                     /locus_tag="RJE42_001911"
     CDS             complement(7910..8671)
                     /locus_tag="RJE42_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV5877324.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(8787..>9937)
                     /locus_tag="RJE42_001912"
     CDS             complement(8787..>9937)
                     /locus_tag="RJE42_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDV5877325.1"
                     /translation="PEPEPTPDPEPSPEPEPEPTPDPEPSPDPDPDSDSDSDSGSDSD
                     SDSDSDSDSDSDSDSDSDSESDSDSDSESDSESDSDSDSDSDSDSDSDSDSDSDSNSD
                     SESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSGSDSDSDSDSDSDSDSDSDLD
                     SDSDSESDSDSDSDSDSDSDSESDSDSDLDSDSDSDSDSDSDSDSDSENDSDSDSDSD
                     SDSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSD
                     SESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKA
                     SKQHQTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT         3195 a         1735 c         1532 g         3475 t
ORIGIN      
        1 atattatttt tcatattgat atttctatac atatatttct gattgtatat ttagatataa
       61 tcatttgtta ttggaaaata tatgaaataa atttgactca tctttttcaa tatccaaatg
      121 acacgatgta tgatttttgt aattaattta cttttaattt cctttaaaat tagactgaat
      181 tgtgacaatt ttcaaacatt ttaatgtgta aatcatcgga aaatatatac gaattgcatt
      241 taaatcgtca atttacatat atttttgcgt agtaatcaat cgttatcatt gttatttatc
      301 gttacattac ttcgagtatc agtatgtatt tcgggcttcg tttgataacg acatttcttt
      361 gtgacatcgc ttcatcagtg taactacaaa tacaatgatt tcgtgatgtt agttacccat
      421 tttatgtgtt gcataaaata tgttgttata aaacatttta aatcatttta tataaacaag
      481 ctatatattt ttgatatttc aaaaatataa cttgtcatac aagtaaaaaa ccgataagac
      541 attttatctt atcggttgaa atttgttatt gttatttgta gtatttttag gttttctttt
      601 tcatataata tatttcagtg aaaacacatg attgattgtg atttcactga aacatgatta
      661 attgcgttgt tgatgaataa ctttagcata aatgtaggaa gttattttgt acatcgccat
      721 atatagaaac gaaattataa tgacagctag tacgtaactt gttaaaaata tatgatggtt
      781 attaatccct atcatattta gtaacgtata aacaatgttg ctagaaatta atgtatgaat
      841 cagtgctact gttattggta ttgcgaacaa gaaagtcatt tgatttcgtg ttatctttga
      901 tattctccca ttatctaaac caagtttttt cataatgtca taattgtgct gatttgcata
      961 ggcttctgaa acttgcttat agtacatcat tagaaatatt ccaatgacaa gtacaattga
     1021 taccacgcca cctacaaata ttagtccacc tgtcagacga tcccaaacca ttagcatttc
     1081 tgatttagaa tttattgatg catcgtactt tttcgaaagc ttttgaattt gtgatgttgt
     1141 taatttcgtc tttccagata cattaaagtt tatcgatatt gtcgaattct tttgttcatg
     1201 tgcataaaat ccttttactt tttgtaattg atttttatct ttaacaatta aaaccatact
     1261 atcttgaatc atcatcaaat ttaacgcatg tgcatcaagt tgtttcactt taaagacttc
     1321 attattaaga ccgaccttag actgcttttt aaaaataggt acattcgtaa tcatacctat
     1381 actttgattg tctaagtgta atttgttttg gtgatatcga ttataatctt gcacagtaag
     1441 tgctgtaatc attactgatt gcttagaaaa tatcgagcct tcattaccaa tgaagtctat
     1501 tggaatatca tcactagcct gttttaactt gtaataagca cgattctttt ctaaagaagc
     1561 tctaaataat gtcgtttcat atactttaaa gtcttttgca ttaaccaaac cctgtatatc
     1621 acgtttaata tgctcaatcg tttgttgacg ttctatttgt gtcttagaat tgtcgttaaa
     1681 tgacagatca taatcattgg taatcaattt cataatggta tgattcatat cgcgataggt
     1741 tgttaatgtc attgtgagcg ttacaatcaa aaatgtacac agcaaagtga ttgttgcaag
     1801 actgactgca ttcattttaa gacgtacacg catcccaact accacaaaaa ataccgtgga
     1861 tgatagtaaa cttttgatac ctgctgcaat atactaataa ttatttcact tatacctaca
     1921 aaaaatgtat aagtaccgat aataactaat cctatgacaa tccatatttt aaaaaaagca
     1981 ccaaacgtcg tatcttgttg taatgcaata aagtagccta aatatagtgc tgcgcttcct
     2041 ataacaatta aaacgtaacg caaccatcgt gatatgacac tggaatcgga acgatgttga
     2101 taagttatcg gtcgttgaaa attaatactg aacaagttga atataagtag catgcccatt
     2161 aatacagcaa tgataatcaa agtaataaac atcgctacag aatcaaatgg ataatacctt
     2221 aacgtagcaa cttcactgcc cattattttc tgtataaaca agaaaaatat cgcaccaagt
     2281 aagtatccgc cggcaatact aatgactgaa attatcataa attgtaagat agtttccatc
     2341 actacaatta aacgcatact tttcttggtc atgcccaatg tcataaaaat actaaattct
     2401 tttcgtcttt gtgacatcat aaagtgattt gcatagaaaa taaaaataaa agttaaaagc
     2461 gccataaaaa aattagctat gataataaat ggtactagga agtcattctt ctgttttatg
     2521 tagctattta acccaattga tacaagcgta tattcaatca tgaatagtac acttaccgct
     2581 aaaatgaatg gaatgattac aagtcgctgc gtaataaatt gacgtcgcac tatgtgccat
     2641 aacaatctta acttcatata ttgacacttc ctccattcac aagtgctaag ctatctgtta
     2701 ttcgttgttg aaaagctaat tgactttctt caccacgata tatttcatga tatagacgcc
     2761 catctttaat aaaaatgaca cgctctgcat aagacgcatc gatatttgaa tgagttacca
     2821 ttaaaattgt ctgttccaat tgatgaatct cttgaaataa catcatcaat gcctttgatg
     2881 tttttgaatc cagtgcacct gtaggttcat cggctagtaa aatcgtcggt ttagtaacta
     2941 atgcacgtgc aatggcaatg cgttgcttct gaccccctga gatttcagaa ggatatttgt
     3001 ttaaaaatcc ctctaaatgt aattgtactg tcaactgatg taccctttgt tctatatctt
     3061 ttcgtttagc accagctaaa attaatggca tcattatatt ttctttattc gtcattgttg
     3121 gtaaaagatt aaaatcttga aaaacaaaac ctaccatttg ttgacgatac aatgctttac
     3181 ttttattttt catattattt aaatgtgcgc catccacaat aatgtcacct tcagttagtc
     3241 catcaaaaga agcaattaaa tttagtagtg tagacttccc tgacccagac tcacccataa
     3301 ttgcaacaaa ttcaccagct ccaactgata aattcatttg attaagtgct gtcgtagcat
     3361 tcaaaccttt accataaacc ttttttacat gtttcacttc tagcaacatg gcatgcacct
     3421 catttctttg ttagttctat attaaaacaa aaaaatacta ataacattta aattagtgaa
     3481 aaaatgttac tagtatgctt acaatattga aagtttttat tcatctggaa attgaatcgt
     3541 aaatgttgta ccctcatttt gtttagatac gactgaaaca ggatggtttg tgtgagttga
     3601 aatttgtttt acgataaata aaccaatccc acttgagtta ctttgccgct ggccattata
     3661 acctgaatag cccttatcaa atattttagg caagtccgct tcactaatac cgataccatt
     3721 atcttttacg tataattgat tggattgctc atcaaattca atccatatat ctttacctct
     3781 cgcatactta agtgcattat ttattaattg ttctatcatt aaagaggtcc atctaacgtc
     3841 agttaatact tcgtgatgac aaggttcata atggattttt gttttttgat caataaactg
     3901 tattgaatat ttcataataa ttgggcgaat gatattgtta atcgaaattt tagtgacaga
     3961 aatatcagaa gtttcattta ataactttaa ataactaagt gctaaacttg tatagttatc
     4021 aatttgaata acctcttgac gaacacgatt cacaacatta ggctcatctc tttcaagtaa
     4081 cagttgtgct gcagtaatgg gtgttttcat ttgatgtacc catgttaaaa agtagctctc
     4141 cacatcattt ttatattcaa tttgttcatt tttaagctga tacaacgcat tttctaagtt
     4201 ttcaatttgt tgttgttggc ttatagtttt tacaaaactt aaatatttaa tacctataaa
     4261 tatgagtaat aataataaaa taacgccaag tgctagtaaa tatgcttcta atggcaaatg
     4321 atacaggtaa aacattaagc caaataaagc aaaaataact atgactattg ctatttcctg
     4381 agtaatactt ttaagaaagg tcattgcatt caccctatac tttatatccg acatttttct
     4441 ttgtaatgat aaaatcatta acgccaatag tatttaattt tttacgcagg cgagtcatgt
     4501 taacagctaa tgtgttatca tctatgaagt tttctgattc ccaacatttt tcaattaaag
     4561 cagttctact tacatattta tcttcatttt gaaataataa ctttaatatt tgtaattcag
     4621 tcaaagatag ctgtatgttt tgttcttgat acacgacttt tgcttcatct aatattaatg
     4681 tacaaccttt aaccgtcatc gaatcattag ctactgacaa gtcataagtt cgtctcaata
     4741 atgcttgaat tttggcaatc gttaatgaca agttaaatgg cttttcgata aaatcatctc
     4801 cccccatttg tattgccata atttggtcca tattatcaat acgggaacta ataaatataa
     4861 ttggcacatt agatgttttt cggatttctt gacaccaatg aaaaccattt aacgttggca
     4921 aattaatatc tagcaataca agctgaggtt gatgttggtt aaagatagac agtatatcat
     4981 caaattgttc aacgacaata acaccgtaat tccatttttc aagttcagat gctaaacttt
     5041 ctgctataac aaaatcatct tcaacaatta atattttcat ctataatctt cttccttcaa
     5101 ttgaattatc ctttgtcatc tttttgttct ttagcctgct gcaatgacgg ctcttgattt
     5161 gaacttcccg tgtcatttgt attttgattt agtgtatttg ctttttcttt ctcgagttgc
     5221 tttttcttcg tacgtttacg ctcttctaaa atagacaaca ctataaagaa tatgattaat
     5281 aatggagata acattactag tatcattttt ccacctcaaa ttatatttaa ttctatattt
     5341 tcacacgctt ctattctaac gctttctata acaaattgtc atatatattc attttttgtt
     5401 attctaaatc actctgttta catattttta actaaaacta cataattgct caaaattcct
     5461 cttttacact gaagacatag tcatatactc aggaggatgt cttatgttat cagttaaaaa
     5521 agccacaaaa tttgcagttg ctaaccttat tatcgtgtca acagttttta gctctgtcac
     5581 acctgcaatt gcgcaatccg ataaaagttc taaagatgaa agtttcgcgg ttggtaatac
     5641 taaaaatcca aaaaatgtta tctttttagt tggcgatggt atgggtccat catttaacac
     5701 agcttatcgt tattataaaa atgatccgca tgctaaagaa atgaccccta ctgcttttga
     5761 ttcatattta aaaggcacaa atcgtacata ttcaaatgac ccaaaacaga atattactga
     5821 ttctgcagct ggcggaacag cctttagttc tggtcataag acatataatg gtgccatcgg
     5881 tgttgacagt aacaagcaga aagtaaagac ggtgcttgaa cgtgcaaaag aaaaagggaa
     5941 gtcgactggt cttgtttcaa cagctgaatt aacagatgca acaccagcag catatgctgc
     6001 acacgtgact tctagagatg ataaaaacga aatcgctaaa caattttata aagataaaat
     6061 caacggaaag cataaagttg atgttttatt aggcggtggc gctaaatatt tcggtaaaag
     6121 taatggcaat ttagataaaa aattcaaaaa agatggttac gaccttgcca cgaacagtaa
     6181 ggagttatca aaatctgata aagataaagt tcttggtttg tttgcagata aaaatatgcc
     6241 actagcaata gatgcttcta aagacgaacc atcattagct gatatgcaac aaagtgcaat
     6301 tagtaagctt gagcgtaata aaaaaggttt ctttttaatg gtcgaaggtg cttctataga
     6361 taaatcagcg cattccaacg atattacagg cgttatgtct gaaatggaag gttttgaaaa
     6421 agcattcgat gacgccattc aatatgctaa gaaacataaa gatactcttg tcattgcaac
     6481 agcagatcac tctactggtg gtctaacaat tggtaaagat aaaggatacg aatggaatcc
     6541 tcaaccgatt aaatcgatga aacactctgg atcatatatg acagaaaaaa tggctaaagg
     6601 tgaagaccct gaaaaagtta ttaatgaagg ctatggcttt aaattttcac aagatgatat
     6661 gaaaaaggtt aaaaaagaga ataaaaagct aaaaagacta cttaaaaaag aaaaagacga
     6721 aaaatccgca gatgtagaaa aacaaacaaa agccttacaa cacgcaattc agaagccagt
     6781 aaatgataaa tcttatacag ggtggacttc cgatggacat acaggcgagg atgttaatac
     6841 ctacgcttat ggacctagat cagaatcatt tagtggtaat atcgaaaata cagaaagcgc
     6901 aaaaataata tttgatatat tcaagtaaag cataaaaaaa ttcccgagca ctacaacaag
     6961 gtgttcggga attttttatg gtttaattta gtactcaatg ttaggtgctt ggttaacacg
     7021 ttcttggtat tttttaaatg cgaagtataa accgccaact aatgttaaaa taactgctaa
     7081 ttttttcatc attcaaactc ctttctaaga tgtgttgaaa ctgagttcaa ttaaattata
     7141 tgtttttatt atacactttt tgacatattt tttaaattta agaatgcgaa gatttttaac
     7201 atttctgatg ctagctttct ttttcatctt tcaaatgctt aataaaatca aagccaggtc
     7261 gccctttagc caatgcttcc atcacatcat accaatccga accgacttgt tgatatatat
     7321 tttgcaataa ttcatcttgc gccttgttca attcagcaat gagttgatcg cctttatccg
     7381 tcgaatataa ttttttgaca cgtcgatcag tctctaaaac tttttcaata atgagacctt
     7441 gctcttttaa tttttgaagt gttgcatgtg atccttgttt agaaatttct aatatttcta
     7501 gtaatgattt aatagtaata ccaggtaatt tattgataaa aaacaaaaaa cgatgatgct
     7561 gacggctcat accatacttc tctataattt catcagcggt agtaataaac gttttatatg
     7621 caaaataaaa caacatatgc tcataatcgt ttttattggt cgacatgatg atgactcctt
     7681 tcaaagcgtt aacctcatta taataaaatt tataatgtaa gtcatacaaa tgatgataaa
     7741 actataagct aaatctaagt ttcacgagaa acattttgtg tatcagcaaa ttgcaccaat
     7801 catattaata caaaattaat ttaaacactc agaaatgtta gtcgtgcgta aatagtttgt
     7861 atcactcact ctgtgtattt aatgtaggca catattttcc aggaaataat taatcattca
     7921 ccatccatgt tatagcacgc ttaatcgctt ggtcccaata tgcataatca tgatctcctg
     7981 gcccatcttc aaattgataa ggaacatttt tgcgtgataa ataatcgata aaatctaaat
     8041 tgtcttgata taaaaagtct tgtttaccac acataatgag caattttgga atttgtttat
     8101 cttcagctac agctttgtct agcaagtaat acggatcatg ttcagttcct ttaacacttg
     8161 aaagattgcc aattatggcc tcttttgaaa aatcattcca ctctagatcc attaaatttt
     8221 gcgcttcaaa cacagcagat aatggtacag ctttggcaaa tttatcccct tgtgttaatg
     8281 caaatttaat tgtgccatat cctcccatag agtgacctgc tataaaattg tcatcacgct
     8341 ttttggaaag tggaaatatt tgatgaacat aatcatatac ttccaaaata taatcataat
     8401 agctatgacc atatgccatg ttagcatacg cgctatgatc cacattgggc ataatcacag
     8461 ctaatttgtg ttcattcgca tacctttcta tgcttgtata tctcatatat gtcgtttcat
     8521 cacttgataa tccatgtaac aacattaaag tttttaatgg tttagcagtt gtatcgttat
     8581 taaagaagct ttgatcttcc ggtaaaatga ctgtcaaatt ttgatgcata ccaattgttg
     8641 gtgaatgata gtttaatgaa atataagcca tacgtcatga cccctttcta attctacttt
     8701 atcaacattt tacgcttaaa caattcactt taaaatcatt ttcaacaaaa aaaccgaata
     8761 caaatgtatt cggcctaaaa aagtatttac gctttttctt tatgatcttg cttgcgtttt
     8821 ctaaacaata gtaatgatcc taataatgcc atcattgcac caaataaagt tgcatttgtg
     8881 ttttcgctct tatctcctgt ttctggtaaa gcatcagttt ggtgttgttt tgatgcctta
     8941 ttagaatggt ttacttcacc tttaggattt gatggtgctt tctgttcatt atttggtggt
     9001 gtaactcttg aatcggagtc gctatctgag tccgaatcgc tgtctgagtc cgagtcgctg
     9061 tctgaatccg aatcgctatc tgaatctgaa tcactgtctg attctgaatc gctgtctgaa
     9121 tctgagtcac tatctgagtc tgagtcactg tctgattctg aatcgctgtc tgaatccgaa
     9181 tcgctatctg aatctgaatc actgtctgat tctgaatcgc tgtctgaatc tgagtcacta
     9241 tctgagtctg agtcactgtc tgattctgaa tcgctatctg aatccgaatc gctgtctgag
     9301 tccgagtcgt tttctgaatc tgagtcacta tctgagtctg agtcgctgtc tgaatccgag
     9361 tcgctatcta aatctgagtc actgtctgat tctgaatcgc tatctgaatc cgaatcgctg
     9421 tctgaatccg agtcactctc tgagtccgag tcactgtcta agtctgagtc actatctgag
     9481 tctgagtcac tgtctgaatc cgagtcactg tctgaacctg agtcactgtc tgagtctgag
     9541 tcgctatctg agtccgaatc gctgtctgag tctgagtcac tgtctgaatc gctatctgaa
     9601 tctgagtcac tgtccgaatc tgactcactg tctgagtttg agtcactatc tgagtctgag
     9661 tcactatctg aatctgagtc actatctgag tccgagtcac tgtctgattc tgagtcactc
     9721 tctgagtctg agtcactgtc tgattctgaa tcgctatctg aatccgagtc actgtctgag
     9781 tccgaatcgc tgtctgaatc cgagtcactg tctgagcctg agtcactgtc cgaatccgaa
     9841 tccggatctg ggtctggact tggttctgga tctggcgttg gttccggttc tggctctgga
     9901 cttggttctg gatctggcgt tggttccggt tctggct
//
LOCUS       DAPIDM010000094         9891 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000094 DAPIDM010000000
VERSION     DAPIDM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.094"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(487..1041)
                     /locus_tag="RJE42_001913"
     CDS             complement(487..1041)
                     /locus_tag="RJE42_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDV5877326.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(1361..2761)
                     /locus_tag="RJE42_001914"
     CDS             complement(1361..2761)
                     /locus_tag="RJE42_001914"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDV5877327.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNKTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLLKKPTQMRDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDDKLVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(2785..3708)
                     /gene="hemH"
                     /locus_tag="RJE42_001915"
     CDS             complement(2785..3708)
                     /gene="hemH"
                     /locus_tag="RJE42_001915"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV5877328.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHKD
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYKQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHITIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(3765..4802)
                     /gene="hemE"
                     /locus_tag="RJE42_001916"
     CDS             complement(3765..4802)
                     /gene="hemE"
                     /locus_tag="RJE42_001916"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV5877329.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(4792..4899)
                     /locus_tag="RJE42_001917"
                     /pseudo
     CDS             complement(4792..4899)
                     /locus_tag="RJE42_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5064..5567
                     /gene="traP"
                     /locus_tag="RJE42_001918"
     CDS             5064..5567
                     /gene="traP"
                     /locus_tag="RJE42_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDV5877330.1"
                     /translation="MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTADSSVIFEETEEN
                     TVLKSPSIYEVIKEIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKS
                     YRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(5691..6914)
                     /locus_tag="RJE42_001919"
     CDS             complement(5691..6914)
                     /locus_tag="RJE42_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877331.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYFNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVTFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIALVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IEIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGNKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(6907..7647)
                     /locus_tag="RJE42_001920"
     CDS             complement(6907..7647)
                     /locus_tag="RJE42_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877332.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            7781..8203
                     /locus_tag="RJE42_001921"
     CDS             7781..8203
                     /locus_tag="RJE42_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDV5877333.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNNAKQQIAEQIQAQF"
     gene            8345..8710
                     /locus_tag="RJE42_001922"
     CDS             8345..8710
                     /locus_tag="RJE42_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDV5877334.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            9442..>9891
                     /locus_tag="RJE42_001923"
     CDS             9442..>9891
                     /locus_tag="RJE42_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDV5877335.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITD"
BASE COUNT         3239 a         1791 c         1373 g         3488 t
ORIGIN      
        1 ttgaattacc taattaatat accataatca aaaacctaaa gtcaagaact ttttgaattt
       61 aatttaaatg ttatctctta aataattact taaatatcgt agcaacatgt tctctgttga
      121 acacaaatat tagtatattc atttttgtag tatacgtcaa cgacattttt aaagtttttt
      181 gtgtaaaaaa acgcttctta ttccctttta tcatataagt gtctaatagt tgtcataaat
      241 agtgttaaag catttaaaaa ggtataggaa ttataaagtt tacaacgcct ataccttctg
      301 aaaaagaaat ttaaaagctt gctacaccgc atgttcattc agtcatcgac taccaatatg
      361 acattgtaac ccctaagaca ttggttgatg cgctaatctc ttcattttgt taaaaacata
      421 atttaccaca attttttcga aatcagcagt tacctactga cacaacataa cacaacaata
      481 cattacctat cgtgtcatat aatcttgcat ccgatcttgc aacgctgtaa atgtttcgaa
      541 gccatcctct tctaagaagt gccctccatc ttccacgatt cgcaagttcc cctctaatgc
      601 attcattaaa cgctgggtct ctttatatga aacgtatttg tcatttttag aactcaatcc
      661 gtaaaaattg tcaactttct ttttaatatt atcgtaatca atggttacat tacttaaatc
      721 aatatctaaa tctatatttt ccgcatcttc tttaaagccc gctatactga aaaaaccttc
      781 aatcggctga tcaatcattt caatatattt taaagctgtg attgaaccta aaccatgtgt
      841 tacaaaatat gtatcctttt tgcgtacatt aatttgtttc gtcatagctt caatccactg
      901 atccactgtc ttcgcttcag gggattcaaa attaaataat gttacgtcat atccttctaa
      961 agttaagtta tgttccaacc actgatacca atgatttcta ctatttccat gcatagaatg
     1021 tacaatgatt acatctgtca tctcattctc tcctttcaac ttactacttc ttttctattt
     1081 aaaaaaatga atgattactt ataattgtaa aataaaaaca ccttaattag aaatgttata
     1141 tcgcaaagtg acatttctaa ttaaagtgca ttgtcatcat ttcaatatca ttcaataata
     1201 gctaaacctt cttcttcgat tcagtttcat gaaataattc ccgctgaaag tatcatgatt
     1261 tacacattac ttccaccatt atataactta aaaatgacta tatttcatca aacattatct
     1321 aaaggcatcg ctcctacatc aacaccatcc aacaattaac ttacaactct gcgattactt
     1381 cttcagcagc aactttacct tgtgtaatac aatcaggtag tccaaccgct tcaaaagatg
     1441 caccagttac tctaagtcgt ggatatgttt gtttaatatg tgcttgaatc tgcctaattt
     1501 gttgaatgtg accaacatgg tactgcggca tacttttcgg caaacgattg acaattgtaa
     1561 attcaggatc gcctttaaat gtcatcattt gacttaaatc tctacgtaca atcgatacta
     1621 atttatcatc tgtatgatca tcaaccacag tatcacctgg tttacctaca tacgcacgaa
     1681 tcaaaacctt accttctggt gtagtaaatg gccatttttt tgatgtccaa gtacatgcgg
     1741 taatgtcagt atcactcgtt ctcgcaatca cgaagccagt accatcataa gtattttcaa
     1801 tgtctttttc atcaaatgcc aatacaacag ttgcaacagt cgtactatcc atcgttttaa
     1861 aataatcaaa tgctggatct tgcccgaacc aattcaaaaa gacttgatgc ggtgttgtca
     1921 ctaatacccc atcgaataca tcttcttgtt gattactgta aacaatttta tattgctttt
     1981 gagatgtaat aatatcatcc actgacgtat tgtagcgtat tgtcacacct ttatttttca
     2041 catcttgttc taatgcttca ataaatgaac ttaaaccatg cttaaattgt ttgaattgtc
     2101 cttttggtgc gccaggatat aattgtcttt gtttcagacg cttatttttc tcatccttca
     2161 taccttttat cagacttccg aatgcctctt ctttttcttt aaaattagga aaggtactca
     2221 tcaaacttaa tttatcaata tcggtaccat aaataccacc cattaaaggc tcaattaaat
     2281 tctcaagtac ctcattacct aatcttgctc tgaaaaatgc accaacagaa atgtcaccat
     2341 cccgcatttg agtaggtttt ttaagtaaat ctaaccctgc tcttaacttt ccaagtggtg
     2401 aaattaattt agttgtcaca aacggtttga tatctgtcgg aattcccata attgatccac
     2461 ctggaatagg atacaatttg tttttcgcaa aaatataaga ttgtccagtc ttatttgtaa
     2521 caatatcttg ttctaatcca atatctttcg ctaattctgt catgatcgtt tttctaccta
     2581 aataagattc aggccctagt tcaatcatat aaccatcttt acgatacgat tgaatctttc
     2641 cccccggacg attcgatgct tcaaagatgg ttacatcaat attaggatct tgctgtttta
     2701 aaaaatatgc acttgataaa cctgttatcc ccgctcctat aatagccact gatttagtca
     2761 caacgtttca cgcttctttc gtcattaaaa tatagacttg atttcatcaa caattgcacc
     2821 gataaataat ggatgtgtat tcggcatttt tggacgataa taattcgcac caatatcatc
     2881 gcaaacaact ttacattcat aatcattgtc ataaagcacc tctaaatgct cacatacaaa
     2941 acctactggc gtatatataa agtttttata ctgatgtttt tcatataaat cacgtgttaa
     3001 atcttgtaca tctggcccta accaaggtgt acctgtatta ccttcagatt gccaaccaat
     3061 cgtgatatgt tcaatattag attgttcttt aattaaaagc gcagtatgtt ctagttcttg
     3121 tggatatgga tcattattct tttcgattag accttttggc aaactatgtg ccgaaacaac
     3181 taataccgtg tctttatgtt cctcttccgg tatttgagct aatgtttcgt tgactttatt
     3241 cgtccaatat tcaataaatt taggttgttt ataataatgt ttcacatgtg taagttgaat
     3301 accatatttt gcagcttctt catcagcacg tttgtcatat gatcctactg aaaatgaaga
     3361 ataatgtggt gctagtacta cagtgattgc ttcagtaata ccatctttgt gcatttgttc
     3421 aaccgcatct tcgataaatg gtgaaatgtg ttttaatcct aagtatagtt taaattcaac
     3481 atctgcatat gctttattta atgctgaaac tagcgcatca gcctggtcat ctgttgtacc
     3541 tgctaatggt gataaaccac ctataaattc atatctatct ttcaaatctt gaagttcttc
     3601 ttcagatgga cgtttaccat gtctaatatc tgtataatat ggctctatgt cactttcttt
     3661 ataaggtgtg ccataagcca taactaataa tcccattttt ttagtcattg ataatacctt
     3721 cctttaaatg aattatcttt catgtcttca atgtaatact atgattatct ttgtgtatat
     3781 gtgtgtacaa attcacttac tttacgtaac gtctctggtt gcacttctgg gaaaacaccg
     3841 tgtcctaaat taaagatgtg tttaccgttc tccatacctt gatctaatat tggtttcaat
     3901 ctctcttcaa tgacattcca tggtgctaat aaaattgatg gatctaaatt cccttgtaat
     3961 gttttagtaa cgcctaattg ttgagcctga ttaatagacg ttctccaatc taggcctaat
     4021 acatcaatcg gcaaatcatt ccattcattg attaaatgac tggcacctac accgaataaa
     4081 attaccggca catcatgttt ttctttaacc tcactgatta atcgaatcat atgtggttta
     4141 atgtaacgtc tgtaatcctc gacatttaat gcacctaccc atgaatcgaa aatttgaatc
     4201 aattcggcac ctgcttcgac ttgagctgtt acatatttaa cagatacatc aactaaatga
     4261 ttcattaaag caaaccatgt tgcttcatct ctatacatca tcgcttttgt aaaattgtaa
     4321 tttttcgatg gtccgccttc aatcatatat gacgctaatg taaatggtgc cccagtaaat
     4381 cctattagcg gcacatttaa cttttcttct gttaaaagtt taattgtatc taatacatat
     4441 ggtacatctc gttcggggtc tatttgagaa agtttctcaa catcttgaat tgttttgata
     4501 ggattatgaa tcactggacc aatacccgat ttgatttcta catcgacacc aattggcttt
     4561 aatggtgtca taatatcttt gtataaaatt gctgcatctg tatgataatt atcaactggt
     4621 aaatgtgtaa cataagcgca caactccggc tgatgggtaa tatcgaatag tgaatatttt
     4681 tctttcaatt ttcgatattc tggttgcgaa cggccagctt gtcgcataaa ccaaacaggt
     4741 gtatgtgtcg tttcttcacc tttaatcatt ttcaaaattg tattgttttt attatgcacc
     4801 ataaaggcct cctaaattaa aatcattctt atctatatta tcatatcgct cattcgttcg
     4861 tattttcaat aaataaatgt cataaaactg acattaaatt acacatcatt tgtttgtgca
     4921 tttaaatttt aaaatcttga tggattatct ccacatcttt ttgtcttgca tgatttatta
     4981 tagtaacttt aaccgtatat taatagtggt aatgactagt ttatcgtcgt ataataaata
     5041 aaaacataag ggggaccttt catatgaaga aattatatac atcttatggc acatatggat
     5101 ttttaaatca gataaaaatc aataatccat ctcatcattt attccaattt tcaactgctg
     5161 attcatcggt gatttttgaa gagactgaag aaaatactgt actgaaatca ccttcaatat
     5221 atgaagttat taaagaaata ggcgcattca atgaagatca tttttattgc gcaattttta
     5281 ttccgtcaac tgaagaccat gtttatcaac ttgaaaagaa attaatcagt gttgatgaca
     5341 atttcaaaaa ctttggtggc tttaaaagtt atcgattgtt gagacctgtt aaaggtacaa
     5401 catacaaaat ttactttggc tttgctgatc gacaaactta tgaagacttt aaaaattctg
     5461 atgcttttaa agatcatttt tcaaaagaag cattaagtca ttactttggt tcaagcggac
     5521 aacattcaag ttattttgaa agatatttat acccaataaa agaatagatt cgaagaaaac
     5581 ttccattggt tatgctgtta acgtatagtt gctaatgtaa tctgaacgcc gtatcatttg
     5641 gcgttcaaca ttacatgcac ctttacaaca tacatgccaa tggaagtttt ttaatctcgt
     5701 aataatgttt cctgatattt taacttttta ataatacttc taatcgttaa taaacctaca
     5761 atatagaaaa tgagtacaac atagaataac gtcatatgtt ttataataaa tgtcactgca
     5821 aacacggtac aaataacaaa cattaatcta tataaaaact gttcataccc tttgattacc
     5881 ttctcttcag gtacgggcca tacttgaggc cacaagccat atgcttgctg tgaataaaat
     5941 tgtgccattt gtaacaatat aatatataca aataaacacc caataattgc tgtcactaat
     6001 ggatatgata accaaaccat aagtaaaact gcaataatta ctaacctaaa gataatatta
     6061 aatgcgtctc tccctcttat aaagcttcta ataaataaga ataaatacat cgcattagag
     6121 ttaaatttat taccctttgg aactggtaaa agtatatcta gataacttct tctgactgca
     6181 gattctttca aatgtttcac atcggtaaac atattaacaa atttataata attcatatga
     6241 tgtcgatgtt cgatttcaat cattttctcc caaggataca aaaagccagg tttatatttt
     6301 ttaactaaaa attctattag cacaggcaaa gcaaccatca caagcgcaat gtaccatttt
     6361 ggagctaata gtaagtaata tgttagagca aagatgatga atgatattaa attaacttgc
     6421 catgttttaa gtcccgattg ataccattgc catcttaagc gtaaaccaac atatggaaaa
     6481 attaatgcac tgactccaaa acaaatataa aatgtcacat tatgttgatt aatattgtaa
     6541 aacaacggga acatcacaat aacaataatg agttggatta atatgcgcgc aaagtaacta
     6601 tataaaatcg catggcgcat aaattgagac atgtgttttt caaatggtaa taaaaagatt
     6661 ttatccgctt cttttaggag tggtcgcatt ggaaaaatgg atgtcaacgc aacaatcact
     6721 gctgctatta atgcaaaatt gatattcgtt ggaatatgtt ttaaccattc accatatcca
     6781 aaaataaatg cacccagcaa aataagtaaa aagaccatga aatgaccatt aaatataaac
     6841 ttattaaaat aatttttctc tttacgaagg gcatgtaatc ttttattaaa taatgtggtc
     6901 gcttggttac gcatgtacat ctccaccttg cgtcacatga atatatatat cgtctaatgt
     6961 ttgattatgt aagccagttt gttgtctcaa tgcttctaaa tctccaaatg caacgacttc
     7021 accttcgtct agtatgataa aacgatcaca ataacgttca gctgtcgcta aaatatgtgt
     7081 actcattaga acggttctac cttcgttttt cttttcaacc attaaatcta acatggattg
     7141 aattcctaat ggatctagac caaggaatgg ttcgtctata atatacaatt cgggattaac
     7201 gataaacgca caaataatca tgactttttg tttcatcccc ttagaaaaat gactcggaaa
     7261 aactttcaat tcattttcta aacggaatgt ctttaataat ggcattgctc gattcatcgc
     7321 ttcatcacga tcaatatcat atgccattgc tgtcatctca atgtgttcct ctaatgtgag
     7381 ttcttcataa ataaccggtg attccggaat ataagataac tttcttctat aagcctctat
     7441 gtcatcatta atgttgatgt ctgaaattga tagagatcct tccataggtg taagcaatcc
     7501 tagcatatgt ttaatcgttg tactcttacc agcgccatta aggccaataa gtccaacgat
     7561 ttcgcctttg tttaattcaa aatttatatc tttaattaca gggcgttttc catatccacc
     7621 tgtaagctgt tctactttaa ctgtcataag gcacctccat gacttatatt gtaccaaaaa
     7681 ttataaaatg ctcatattaa atacacatgt cctaatatcg aatttttagc gacaatgtta
     7741 taatgaatgg taatacaact tgaaaaggag tgtagtcatc atgtcagaaa caattttcgg
     7801 caaaatttta actggagaaa ttcctagctt taaagtatat gaagacgatt atgtctatgc
     7861 ctttttagat atatcacaag ttactaaagg acatacgtta ttaattccta aaaaagcttc
     7921 tgctaatatc tttgaaactg atgaagaaac aatgaaacat atcggtgcag cattacctaa
     7981 agtagcaaat gctattaagc gtgcatttaa tcctgatggt ttaaacatta ttcaaaataa
     8041 tggtgagttt gcagatcaat ctgtatttca tattcatttc cacttaattc ctcgatatga
     8101 aaatgatatt gatggatttg gttataagtg ggaaacacat gaagatattt tagataacaa
     8161 tgcaaaacaa caaattgctg aacaaattca agcacaattt taaatgtatg cttaatctaa
     8221 gctcgaacgg gtataatatg attaatatta taacaattgc gtttgaagtg ataacatcaa
     8281 ggttagcaat tttaaacaaa atgagttatc aagataacag atgttaaaag tgaggagaat
     8341 ataaatgaaa gcatcacgca ttctattcgg tatcggtgtt ggcgtagcag ctggttttgt
     8401 agttgcactc caaggacgag acgacaaaag tgtcaagaac aacacgatcg atcgtactgc
     8461 ccctactggt tcaaaatcag aactacaacg tgaatttgaa acaattaaac aaagttttaa
     8521 tgacatttta aactatggtg ttcaaattaa aaacgaaagt gcggaatttg gtagttcaat
     8581 tggtggtgaa attaagtcat tacttggaaa cttcaaatct gacatcaatc ctaatattga
     8641 acgtttacag tcacacatcg aaaatttaca aaatcgtggc gaggatattg gaaacgaaat
     8701 ttctaaatag catgttacgt tctcgatcac aactattttt aatagtaaca acatatttat
     8761 tttttaaaat taaatgccaa ataaacgaga tgacattaga aattagatat ttcttgtcat
     8821 ctctttttta tggctcgaat gaacgtttga ttaccaatta cagtaagacg ttgtttgtag
     8881 tgattttccc ttaaatcata tttatgaatt gattgaaaat attgcttagg attcattgtg
     8941 ttatccttgc actttgatta cgctttactt aaatcattat cgacaaacaa catacttata
     9001 ttttcattga gccgaacctt aaatacacat tacatatacc ttacttgcac aaattattaa
     9061 tctggtgttt attataatta catatcacta tatttttagc atttgtatga cttagttagt
     9121 taaaagatgc ttttgcatat gctctgccat atccattttt tatattattc aagttttcaa
     9181 atactcatgt cacacgcgcc tttttcataa gtgatgcttt attagcaaga atatgtgttc
     9241 gcagaaattt attctgcatt ctacttctac gctagtcaat cagacaattt taccaatccc
     9301 actttcgcgt ttcaaatcaa acaatacgtc gcccctttct tcatatataa caattcttct
     9361 aacatgatat gttactattg aattactgaa cctgagttag ttataatcta acttatattg
     9421 aaaagagatg aggcgtaaga tatgttttta tgtaaaagac aaattgatat caatgcacga
     9481 tttggtttgc ctagaattgc atttatgagt gcagttgcaa ccatcattat gtttttagtt
     9541 agttatgaag taatgtattt tttatctaat acgccattat cagatagaca ttttctcatc
     9601 tttttattac ttgtatttat gacgtatcca ttacataaaa gtatacattt attatttttc
     9661 ttaccatata gaaaatcgtt taaagttcat aagttgacta aaagaaaatg gcttatattc
     9721 tataatacct acgtcaatca acctgtacac aaattttatt tttgcattaa cttaatattg
     9781 ccgttaatta tcttatctgc aatgttcgtt tatctaacaa tttcattccc gcaatatgga
     9841 cattatttta tgttcttatt ggcattgaat ttcggtattt ctattacaga t
//
LOCUS       DAPIDM010000095         9852 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000095 DAPIDM010000000
VERSION     DAPIDM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.095"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            140..1624
                     /locus_tag="RJE42_001924"
     CDS             140..1624
                     /locus_tag="RJE42_001924"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDV5877336.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQPLPANVTQVVVPDTLRVASILAHTLFDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYIVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            1605..1865
                     /locus_tag="RJE42_001925"
     CDS             1605..1865
                     /locus_tag="RJE42_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDV5877337.1"
                     /translation="MALLVNKRLIDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMA
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1865..3427
                     /locus_tag="RJE42_001926"
     CDS             1865..3427
                     /locus_tag="RJE42_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV5877338.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEDTLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEIEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3729..4532
                     /locus_tag="RJE42_001927"
     CDS             3729..4532
                     /locus_tag="RJE42_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV5877339.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4751..7075
                     /locus_tag="RJE42_001928"
     CDS             4751..7075
                     /locus_tag="RJE42_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDV5877340.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQGSKSAYN
                     KDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNTEATLSTNSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHAKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNSVPILSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVLREDHIVDDNPDNDINTQSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRSSEKRNIAINDNDKLNGQKPNTKTSANNSQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKYGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIANLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7092..8450
                     /locus_tag="RJE42_001929"
     CDS             7092..8450
                     /locus_tag="RJE42_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDV5877341.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVVAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8589..>9852
                     /locus_tag="RJE42_001930"
     CDS             8589..>9852
                     /locus_tag="RJE42_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV5877342.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EEIGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLNGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQ"
BASE COUNT         3517 a         1319 c         1934 g         3082 t
ORIGIN      
        1 tcataatacc gtatgtttta tttttaatag tagaaattag aaaatgctga ttagtaggat
       61 ataacagtga aattataaat ttattaacat caacaaaacg tgtataataa acatattgta
      121 gaaaaaggag cggttcagtt tggatgcaag tacgttgttt aagaaagtaa aagtaaagcg
      181 tgtattgggt tctttagaac aacaaataga tgatatcact actgattcac gtacagcgag
      241 agaaggtagc atttttgtcg cttcagttgg atatactgta gacagtcata agttctgtca
      301 aagtgtagct gatcaagggt gtaagttggt agtggtcaat aaagaacaac cattaccagc
      361 taacgtaaca caagtggttg tgccggacac attaagagta gctagtattc tagcacacac
      421 attatttgat tatccgagtc atcagttagt gacatttggt gtaacgggta caaatggtaa
      481 aacctctatt gcgacgatga ttcatttaat tcaaagaaag ttacaaaaaa atagtgcata
      541 tttaggaact aatggtttcc aaattaatga aacaaagaca aaaggtgcaa atacgacacc
      601 agaaacagtt tctttgacta agaaaattaa agaagcagtt gatgcaggcg ctgaatccat
      661 gacattagaa gtatcaagcc atggcttagt attaggacga ctgcgaggcg ttgaatttga
      721 cgttgcaata ttttcaaatt taacacaaga ccatttagat tttcatggca caatggaagc
      781 atacggacac gcgaagtctt tattgtttag tcaattaggt gaagatttgt cgaaagaaaa
      841 gtatatcgtg ttaaacaatg acgattcatt ttctgagtat ttaagaacag tgacgcctta
      901 tgaagtattt agttatggaa ttgatgagga agcccaattt atggctaaaa atattcaaga
      961 atctttacaa ggtgtcagct ttgattttgt aacgcctttt ggaacttacc cagtaaaatc
     1021 gccttatgtt ggtaagttta atatttctaa tattatggcg gcaatgattg cggtgtggag
     1081 taaaggtaca tctttagaaa cgattattaa agctgttgaa aatttagaac ctgttgaagg
     1141 gcgattagaa gttttagatc cttcgttacc tattgattta attatcgatt atgcacatac
     1201 agctgatggt atgaacaaat taatcgatgc agtacagcct tttgtaaagc aaaagttgat
     1261 atttttagtt ggtatggcag gcgaacgtga tttaactaaa acgcctgaaa tggggcgagt
     1321 tgcctgtcgt gcagattatg tcattttcac acctgataat ccggcaaatg atgacccgaa
     1381 aatgttaaca gcagaattag ccaaaggtgc gacacatcaa aactatattg aatttgatga
     1441 tcgtgcagaa gggataaaac atgcaattga catagctgag cctggggata ctgtcgtttt
     1501 agcatcaaaa ggaagagaac catatcaaat catgccaggg catattaagg tgccacatcg
     1561 agatgattta attggccttg aagcagctta caaaaagttc ggtggtggcc ctgttggtca
     1621 ataaaagact tattgatgaa ggtaaaacta ttgatgttta tttattcgaa gcattaaatg
     1681 accagataat cattgctata ccagattggt tttggtcata tcagatggca atggcattag
     1741 atgaagaaac ttgttttgaa gcaatactta tgcaattgtt tgtttttaaa gaagaggaag
     1801 aggcagagtc gattgcatca caactaacag attggataga aacatataaa aaggagaaag
     1861 actaatgaac ttaaagcaag aagttgagtc tagaaagact tttgcgatta tttcacatcc
     1921 cgatgcaggg aaaacaacgt taactgaaaa actattgtac ttcagtggtg ccattcgtga
     1981 agcgggtaca gttaaaggga agaagactgg taaatttgcg acaagtgact ggatgaaagt
     2041 tgaacaagag cgtggtattt ctgtaacaag ttcagtaatg caatttgatt acgatgatta
     2101 taaaatcaat atcttagata caccaggaca tgaagacttt tcagaagata cgtatagaac
     2161 attaatggca gttgacagtg ctgtaatggt catagactgt gcaaaaggta ttgaaccaca
     2221 aacattgaag ttatttaaag tttgtaaaat gcgtggtatt ccaatcttta cattcattaa
     2281 taaattagac cgagtaggta aagaaccatt tgaattatta gatgaaatcg aagatacatt
     2341 aaatattgaa acatacccta tgaattggcc aattggtatg gggcaaagtt tctttggcat
     2401 cattgataga aagtctaaaa caattgaacc atttagagat gaagaaaata tattacattt
     2461 gaatgatgat tttgagttgg aagaagatca tgcaattaca aatgatagtg cttttgaaca
     2521 agcgattgaa gaattaatgt tggttgaaga agcgggtgaa gcctttgata atgacgcgct
     2581 gttgagtgga gacttaacac ctgtattttt cggttcagct ttagctaact ttggtgtaca
     2641 gaatttctta aatgcatatg ttgattttgc gccgatgcca aatgcgagac aaacaaaaga
     2701 agagattgaa gtaagcccgt ttgatgattc attttcagga tttatcttta aaattcaagc
     2761 caacatggac cctaaacacc gtgatagaat tgcctttatg cgtgtcgtta gtggtgcatt
     2821 tgaacgtggt atggatgtta ctttgcaacg tactaataaa aagcaaaaga tcacacgttc
     2881 aacgtcattt atggcagacg ataaagaaac tgtgaatcat gctgtagcag gcgatatcat
     2941 tggactatat gatacaggta attatcaaat tggagatact ttagttggtg gaaaacaaac
     3001 ctacagtttc caagatttac cacaatttac gccagaaatt tttatgaaag tttctgctaa
     3061 aaacgttatg aaacagaagc atttccataa aggtattgaa caattagtac aagaaggtgc
     3121 gattcaatac tataaaacat tacacacaaa ccaaattatt ttaggtgctg ttggtcagtt
     3181 acaatttgaa gttttcgaac atagaatgaa aaatgaatat aatgttgatg ttgttatgga
     3241 gccagtaggc cgtaaaattg cacgttggat tgaaaatgaa gaccaaatta cagataagat
     3301 gaacacatca agatcgattt tagtgaaaga tagatatgac gatttagtat tcttatttga
     3361 aaatgaattt gcaacaagat ggttcgaaga gaaattccct gaaattaaat tgtatagttt
     3421 actttaacgg ctcaatagta taatcgaatt tgtgacttta aaaataattg tttcgttgaa
     3481 gaaaaataaa ttgtatattt taaaagaaaa aggtatacta tgatgtatca aatgaataac
     3541 ctatggcatt ttgtcagagg ggagtaactt aagaatcatg accgtataaa tgattcgaca
     3601 ctttatcgtc attacgaaga tatcttccgg taaagtgggc aatttaaatt gcttagtgag
     3661 acctttgcta tttatttagc ataggtcttt ttgtttgtac ttaacttatt tatttaaagg
     3721 agttgtacat tttaatggat ccaagtttga tcttacctta tttatgggta cttgtcgttt
     3781 tagtattttt agaaggctta ttagcagcag ataacgcgat tgttatggct gtaatggtta
     3841 agcacttacc acccgaacaa cgtaaaaaag ctttgtttta cggtttgtta ggtgcatttg
     3901 tatttagatt tttagcatta ttcttaatta gtattatcgc gaacttttgg tttattcaag
     3961 ctgcaggagc ggtttactta atttatatgt caatcaaaaa tctgtggcag ttctttaaac
     4021 acccagaaat tgaaagtcct gaagctggag atgatcatca ttatgatgaa tctggtgaag
     4081 agattaaagc aagtaacaaa tcattctggg gaactgtgtt gaaaatagaa tttgcagata
     4141 tcgcatttgc cattgattct atgcttgctg ctttagctat tgctgtaaca cttcctaaag
     4201 ttggtattca ctttggtggt atggacttag gtcagttcgt agtcatgttc ctaggtggaa
     4261 tgattggtgt tattctaatg cgttatgcag caacatggtt cgtagagcta ttaaacaaat
     4321 atccaggact tgaaggtgca gccttcgcga ttgttggttg ggtaggtgtt aagttagttg
     4381 tcatggtatt agcgcaccca gacattgctg tattgcctga gcacttccca catggcgtat
     4441 tatggcaatc tattttctgg acggtactaa ttggattagt aattatcggt tggttaggtt
     4501 cagttgttaa aaataaaaaa tcgcataaat aaatgatgtg aagcggacaa tcttaattta
     4561 gtttaaggtt gtcctttttc atttaattga gtgatttatg aaaaatggat tttgaagaat
     4621 gtgaatcaaa agatgcgata tagtattaag aaaatgtgcc ttttatattt agcatttttt
     4681 caatagaaat tatatagatt ttaaagcaaa ttaagtgtta atgtgtcata atgaaaagtg
     4741 attttattga atggagtgga cattagtgga tattggtaaa aaacatgtaa ttcctaaaag
     4801 tcagtaccga cgtaagcgtc gtgaattctt ccacaacgaa gacagagaag aaaatttaaa
     4861 tcaacatcaa gataaacaaa atatagataa tacaacatca aaaaaagcag ataagcaaat
     4921 acataaagat tcaattgata agcacgaacg ttttaaaaat agtttatcat cgcatttaga
     4981 acagagaaac cgtgatgtga atgagaacaa agctgaagaa agtaaaagta atcagggtag
     5041 taagtcagca tataacaaag atcattattt aacagacgat gtatctaaaa aacaaaattc
     5101 attagattca gtagaccaag atacagagaa atcaaaatat tatgagcaaa atactgaagc
     5161 gactttatca actaattcaa ccgataaagt agaatcaact gacatgagaa agctaagttc
     5221 agataaaaac aaagttggtc atgaagagca acatgtactt tctaaacctt cagaacatgc
     5281 taaagagact agaattgatt ttgagtcttc aagaactgat tcagacagct cgatgcagac
     5341 agagaaaata aaaaaagaca gttcagatgg aaataaaagt agtaatctga aatctgaagt
     5401 aatatcagac aaatcaaatt cagtaccaat attgtcggaa tctgatgatg aagtaaataa
     5461 tcagaagcca ttaactttgc cggaagaaca gaaattgaaa agacagcaaa gtcaaaatga
     5521 gcaaacaaaa acctatacat atggtgatag cgaacaaaac gacaagtcta atcatgaaaa
     5581 tgatttaagt catcatacac catcgataag tgatgataaa gataacgttt tgagagaaga
     5641 tcatattgtt gacgataatc ctgataatga tatcaataca caatcattat caaaaataga
     5701 tgacgatcga aaacttgatg aaaaaattca tgtcgaagat aaacataaac aaaatgcaga
     5761 ctcgtctgaa acggtgggat atcaaagtca gtcaagtgca tctcatcgta gctctgaaaa
     5821 aagaaatatt gctattaatg ataatgataa attaaacggt caaaaaccaa atacaaagac
     5881 atcggcaaat aatagtcaaa aaaaagctac atcaaaattg aacaaagggc gtgctacaaa
     5941 taataattat agtgacattt tgaaaaagtt ttggatgatg tattggccta aattagttat
     6001 tctaatgggt attattattc taattgttat tttgaatgcc atttttaata atgtgaataa
     6061 aaatgatcgc atgaatgata ataatgatgc agatgctcaa aaatatacga caacgatgaa
     6121 aaatgccaat aacacagtta aatcggtcgt tacagttgaa aatgaaacat caaaagattc
     6181 atcattacct aaagataaag catctcaaga cgaagtagga tcaggtgttg tatataaaaa
     6241 atatggagat acgttatata ttgttacgaa tgcacacgtt gtcggtgata aagaaaatca
     6301 aaaaataact ttctcgaata ataaaagtgt tgttgggaaa gtgcttggta aagataaatg
     6361 gtcagattta gctgttgtta aagcaacttc ttcagacagt tcagtgaaag agatagctat
     6421 tggagattca aataatttag tgctaggaga gccaatatta gtcgtaggta atccacttgg
     6481 tgtagacttt aaaggcactg tgacagaagg tattatttca ggtctgaaca gaaatgttcc
     6541 tattgatttc gataaagata ataaatatga tatgttgatg aaagctttcc aaattgatgc
     6601 atcagtaaat ccaggtaact cgggtggtgc tgtcgtcaat agagaaggaa aattaattgg
     6661 tgtagttgca gctaaaatta gtatgccaaa tgttgaaaat atgtcatttg caatacctgt
     6721 taatgaagta caaaagattg taaaagattt agaaacaaaa ggtaaaattg actatccgga
     6781 tgtaggtgtt aaaatgaaga atattgccaa tctaaatagt tttgaaagac aagcagttaa
     6841 attgccagga aaagttaaga acggtgttgt tgtagatcaa gttgacaaca atggtttagc
     6901 agatcaatct ggtctgaaaa aaggtgatgt aattactgaa ttagatggca aacttttaga
     6961 agatgattta cgctttaggc agattatatt tagtcataaa gatgacttga aatcaattac
     7021 agcgaagatt tatagagatg gtaaagaaaa agaaattaat attaaactaa aataatgttg
     7081 aggtgaacaa cgtgtcaatt tttagccagt ttttaaaaag atcaagccct caacaaggta
     7141 ttgtattgta ctatatcgtc gcaattgtcg ttgcattttt attattaaac ttaccgtatg
     7201 ttcataaacc aggtgtagaa gtaaatccaa ttgacacatt atttgttgcc gtatccggaa
     7261 ttagtgttac tggattgtct ccgataagta ttgtcgatac ctattctaca tttggacaat
     7321 taattatcct tgtgatatta aatattggtg gaattggcgt catggcaatt ggtacgatgt
     7381 tatgggtggt actaggtaaa catattggaa ttagagaacg tcagttaatt atgttagata
     7441 ataacaaaaa cacaatgagt ggtaccgtca aattgattat tgatattgta aaatcaattt
     7501 ttgtaatcga actcgtagga gccatgttat tagcgtttta cttttatcga gataatccag
     7561 atttaaaata tgcaatcatg caaggtgttt ttgtttctat ttctgccact accaatggtg
     7621 gattagatat tacaggtaag tcattaattc cttatgcaca tgattatttt gtacaagcga
     7681 tagttatatt tttaataatt ttaggatcaa tcggcttccc agtattatta gaagttaaag
     7741 cttatattca aaatagggtt actaatttta gattttcatt atttactaaa attacgacat
     7801 caacatattt attcctattt attgttgggg tattagccat tctattattt gaacataacc
     7861 atgcgttcaa aggattaagt tggcatcaat cgttattcta ttcgctgttt caatcagcga
     7921 ctacaagaag tgcgggtctt caaacaattg atgtgacaac actaagtgac cctacgaata
     7981 ttatcatggg tattttaatg tttataggtt cttcgccaag ttcggttggt ggcggtattc
     8041 gtacaacaac tttcgctatt ttaattttgt ttttaattaa ctttagtaat aatgccgata
     8101 aaacatccat taaagtttac aatagagaag tacacattat ggatattcaa cgttcatttg
     8161 cagtatttac aatggcgaca attttaacat ttttaggaat gctaattata tcagctactg
     8221 aaaatggtaa gcttacattt ttacaagtat tttttgaagt catgtctgca tttggaactt
     8281 gtggactatc gcttggtgtc acaagtgata ttagtgatat ttccaaggtt gtactaatgg
     8341 tactcatgtt tataggacga gttggcttaa tatcatttat cattatgata gcaggacgtc
     8401 gagaaccaga taaattccat tatccaaaag aacgtattca aataggataa tataatagca
     8461 atctaagttt agtttatgta gattttaact ggaacttaga ttgctttttt agtttgtatt
     8521 tttaacttat actataagac gattggattt gaaaatggta aactagtaac aatgagaggt
     8581 gtaacatgat ggaaaaaaat gaaaacatta atgtagagat tttaactacg tcagatatgc
     8641 atagtcattt cttaaatggt gattatggtt caaatattta tagagctggt acttatgtta
     8701 accaagtaag agcacaaaat catcgcgtca ttttattaga tagtggcgga agtttagctg
     8761 gctcgttagc ggcctattat tatgctatag ttgcacctta taaacgacat ccaatgataa
     8821 agttgatgaa cagaatgcat tatgatgcta gcggtgtgag tccaagtgaa ttcaagtttg
     8881 gtttatcatt tttaactcgt tcaattgcat tggcacgttt tccatggtta tcagcaaata
     8941 ttgaatacaa tgttactaag gagccttatt tttcaactcc atattgtatt aaacattttg
     9001 gtgacttaaa aattgctatc gtaggcgtta cagcagatgg tttaatggaa aatgagtatt
     9061 ctgaaatgga gcaagatgta tctattgaaa agacattagt ggcatcaaaa cgatggatta
     9121 gatatatcca tgaagttgaa gagccggatt ttttgattgt aatttatcat ggtggattga
     9181 ataaaattag taatagcacg aaaaataaaa aggcaagttc gaatgaagct gaaaaattaa
     9241 tggaagaaat tggtgttata gatttaatga ttacagctca tcagcatcaa acaatagtag
     9301 gtcaagatca tgaaacgtat tatgttcagg ctggtcaaga tgcaaaagag cttgtacatc
     9361 tttcgattaa ttttaaaaag agaacaacaa cttatgatgt tgaaagcatt gattctaaag
     9421 tgattgactt aaatgagtat gaagaggacc aagaattatt agatttaaca ttctatgata
     9481 gaaaagcagt ggcttattgg tcacaggaaa tcataagtga taaaggtttg atgttatcag
     9541 taaatgggtt acaagattta gtctgtcaaa cacatccatt ttcgcaatta ttacatgatg
     9601 caattcacct tgcatttgat aatgatataa catgtgtcca cgtgcctatg aacggagaga
     9661 aggggttgaa tggacagatt cgaaatgaag atttgtatca tgcataccct tatccagata
     9721 agccaatgga tatgacaatt agtggtcaaa atatcaaaga tatattggag tatagttatt
     9781 cacatttaga ttttgttaac gagcaattaa gcttaacaat tattgatgaa acattatgta
     9841 caatgtggca ag
//
LOCUS       DAPIDM010000096         9851 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000096 DAPIDM010000000
VERSION     DAPIDM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.096"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..455
                     /gene="rpsG"
                     /locus_tag="RJE42_001931"
     CDS             <1..455
                     /gene="rpsG"
                     /locus_tag="RJE42_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV5877343.1"
                     /translation="VPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILYSAFDLV
                     EQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLRWLVNYA
                     RLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            578..2659
                     /gene="fusA"
                     /locus_tag="RJE42_001932"
     CDS             578..2659
                     /gene="fusA"
                     /locus_tag="RJE42_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV5877344.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            2876..4060
                     /gene="tuf"
                     /locus_tag="RJE42_001933"
     CDS             2876..4060
                     /gene="tuf"
                     /locus_tag="RJE42_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV5877345.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(4342..5517)
                     /locus_tag="RJE42_001934"
     CDS             complement(4342..5517)
                     /locus_tag="RJE42_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV5877346.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLN
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLNGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSKIQEHVMHKMDKL
                     LQGLAIANDIEYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            5688..6875
                     /locus_tag="RJE42_001935"
     CDS             5688..6875
                     /locus_tag="RJE42_001935"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV5877347.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGTNGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMASTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIVAYEKVGKEM
                     NLI"
     gene            7144..8022
                     /gene="hchA"
                     /locus_tag="RJE42_001936"
     CDS             7144..8022
                     /gene="hchA"
                     /locus_tag="RJE42_001936"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV5877348.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPITDFDGVEH
                     KGAYIDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPAKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALENDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            8180..9817
                     /locus_tag="RJE42_001937"
     CDS             8180..9817
                     /locus_tag="RJE42_001937"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV5877349.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKDTMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQIPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
BASE COUNT         3398 a         1546 c         1912 g         2995 t
ORIGIN      
        1 cagtacctaa aagagacgta ttaccagatc caattcataa ctctaagtta gtaactaaat
       61 taattaacaa aattatgtta gatggtaaac gtggaacagc acaaagaatt ctttattcag
      121 cattcgacct agttgaacaa cgcagtggtc gtgatgcatt agaagtattc gaagaagcaa
      181 tcaacaacat tatgccagta ttagaagtta aagctcgtcg tgtaggtggt tctaactatc
      241 aagtaccagt agaagttcgt ccagagcgtc gtactacttt aggtttacgt tggttagtta
      301 actatgcacg tcttcgtggt gaaaaaacga tggaagatcg tttagctaac gaaattttag
      361 atgcagcaaa taatacaggt ggtgccgtta agaaacgtga ggacactcac aaaatggctg
      421 aagcaaacaa agcatttgct cactaccgtt ggtaagataa aagcttttac cctgagtgtg
      481 ttctatatta atgaattttc attaagcgtt catgcttagg gcatcgccat atctatcgta
      541 tttattcagt aatataaact ggaaggagaa aaaatacatg gctagagaat tttcattaga
      601 aaaaactcgt aatatcggta tcatggctca cattgatgct ggtaaaacga ctacgactga
      661 acgtattctt tattacactg gccgtatcca caaaattggt gaaacacacg aaggtgcttc
      721 acaaatggac tggatggagc aagaacaaga ccgtggtatt actatcacat ctgctgcaac
      781 aacagcagct tgggaaggtc accgtgtaaa cattatcgat acacctggac acgtagactt
      841 cactgtagaa gttgaacgtt cattacgtgt acttgacgga gcagttacag tacttgatgc
      901 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caggctacaa cttatggtgt
      961 tccacgtatc gtatttgtaa acaaaatgga caaattaggt gctaacttcg aatactctgt
     1021 aagtacatta catgatcgtt tacaagctaa cgctgctcca atccaattac caattggtgc
     1081 ggaagacgaa ttcgaagcaa tcattgactt agttgaaatg aaatgtttca aatatacaaa
     1141 tgatttaggt actgaaattg aagaaattga aattcctgaa gaccacttag atagagctga
     1201 agaagctcgt gctagcttaa tcgaagcagt tgcagaaact agcgacgaat taatggaaaa
     1261 atatcttggt gacgaagaaa tttcagtttc tgaattaaaa gaagctatcc gccaagctac
     1321 tactaacgta gaattctacc cagtactttg tggtacagct ttcaaaaaca aaggggttca
     1381 attaatgctt gacgctgtaa ttgattactt accttcacca ctagatgtta aaccaattat
     1441 tggtcaccgt gctagcaacc ctgaagaaga agtaatcgcg aaagcagacg attcagctga
     1501 attcgctgca ttagcgttca aagttatgac tgacccttat gttggtaaat taacattctt
     1561 ccgtgtgtac tcaggtacaa tgacatctgg ttcatacgtt aagaactcta ctaaaggtaa
     1621 acgtgaacgt gtaggtcgtt tattacaaat gcacgctaac tcacgtcaag aaatcgatac
     1681 tgtatactct ggagatatcg ctgctgcggt aggtcttaaa gatacaggta ctggtgatac
     1741 tttatgtggt gagaaaaatg acattatctt ggaatcaatg gaattcccag agccagttat
     1801 tcacttatca gtagagccaa aatctaaagc tgaccaagat aaaatgactc aagctttagt
     1861 taaattacaa gaagaagacc caacattcca tgcacacact gacgaagaaa ctggacaagt
     1921 tatcatcggt ggtatgggtg agcttcactt agacatctta gtagaccgta tgaagaaaga
     1981 attcaacgtt gaatgtaacg taggtgctcc aatggtttca tatcgtgaaa cattcaaatc
     2041 atctgcacaa gttcaaggta aattctctcg tcaatctggt ggccgtggtc aatacggtga
     2101 tgttcacatt gaattcacac caaacgaaac aggcgcaggt ttcgaattcg aaaacgctat
     2161 cgttggtggt gtagttcctc gtgaatacat tccatcagta gaagctggtc ttaaagatgc
     2221 tatggaaaat ggtgtcttag caggttatcc attaattgat gttaaagcta aattatatga
     2281 tggttcatac catgatgtcg attcatctga aatggccttc aaaattgctg catcattagc
     2341 acttaaagaa gctgctaaaa aatgtgatcc tgtaatttta gaaccaatga tgaaagtaac
     2401 tattgaaatg cctgaagagt acatgggtga tatcatgggt gacgtaacat ctcgtcgtgg
     2461 acgtgttgat ggtatggaac ctcgtggtaa tgcacaagtt gttaatgctt atgtaccact
     2521 ttcagaaatg ttcggttatg caacatcatt acgttcaaac actcaaggtc gcggtactta
     2581 cactatgtac ttcgatcact atgctgaagt tccaaaatca atcgctgaag atattatcaa
     2641 gaaaaataaa ggtgaataat ataacttgtt ttgactagct agcctaggtt aaaatacaag
     2701 gtgagcttaa atgtaagcta tcatctttat agtttgattt tttggggtga atgcattata
     2761 aaagaattgt aaaattcttt ttgcatcgct ataaataatt tctcatgatg gtgagaaact
     2821 atcatgagag ataaatttaa atattatttt taattagaat aggagagatt ttataatggc
     2881 aaaagaaaaa ttcgatcgtt ctaaagaaca tgccaatatc ggtactatcg gtcacgttga
     2941 ccatggtaaa acaacattaa cagcagcaat cgctactgta ttagcaaaaa atggtgactc
     3001 agttgcacaa tcatatgaca tgattgacaa cgctccagaa gaaaaagaac gtggtatcac
     3061 aatcaatact tctcacattg agtaccaaac tgacaaacgt cactacgctc acgttgactg
     3121 cccaggacac gctgactacg ttaaaaacat gattactggt gctgctcaaa tggacggcgg
     3181 tatcttagta gtatctgctg ctgacggtcc aatgccacaa actcgtgaac acattctttt
     3241 atcacgtaac gttggtgtac cagcattagt agtattctta aacaaagttg acatggttga
     3301 cgatgaagaa ttattagaat tagtagaaat ggaagttcgt gacttattaa gcgaatatga
     3361 cttcccaggt gacgatgtac ctgtaatcgc tggttcagca ttaaaagctt tagaaggcga
     3421 tgctcaatac gaagaaaaaa tcttagaatt aatggaagct gtagatactt acattccaac
     3481 tccagaacgt gattctgaca aaccattcat gatgccagtt gaggacgtat tctcaatcac
     3541 tggtcgtggt actgttgcta caggccgtgt tgaacgtggt caaatcaaag ttggtgaaga
     3601 agttgaaatc atcggtttac atgacacatc taaaacaact gttacaggtg ttgaaatgtt
     3661 ccgtaaatta ttagactacg ctgaagctgg tgacaacatt ggtgcattat tacgtggtgt
     3721 tgctcgtgaa gacgtacaac gtggtcaagt attagctgct cctggttcaa ttacaccaca
     3781 tactgaattc aaagcagaag tatacgtatt atcaaaagac gaaggtggac gtcacactcc
     3841 attcttctca aactatcgtc cacaattcta tttccgtact actgacgtaa ctggtgttgt
     3901 tcacttacca gaaggtactg aaatggtaat gcctggtgat aacgttgaaa tgacagtaga
     3961 attaatcgct ccaatcgcga ttgaagacgg tactcgtttc tcaatccgtg aaggtggacg
     4021 tactgtagga tcaggcgttg ttactgaaat cattaaataa tttctaattt cttagatttt
     4081 atataaaaag aagatccctc aatcgagggg tcttttttta atgtgtaaat tttgtaatgg
     4141 ttattcgatt tagaagaaca ataattgatg aaagactgac taataaaact tataactgat
     4201 aatactggtt aaataaaatt gttaagtatt ggacattgaa aaatgctcct ctcaaagctt
     4261 tcattttttc aatgtctact ttgaagggag catttcatta gtttatgtct cagattcata
     4321 tctttcaatt aatttaaatg cttaatttgt tttaaatact tgctctaatt caatgatttt
     4381 caaaaataca gctacagcgt attttaatga tttttcatca atatcaaatt tgggattatg
     4441 gtgtggcgct gtaatacctt tactttcatt accacaacca gtcagaaaga atgcacctgg
     4501 tcgtactttc aaataatgtg aaaaatcttc tccaatcatc attaaatctg attcattaaa
     4561 gcgtacatgt aagtcatttg ttgcttcttt aataacttga tatgctttct cgttgttatg
     4621 gacaggcaaa tatcctttaa tataattcaa gtcatattca atgtcatttg caattgccaa
     4681 accttgtagt agcttatcca ttttatgcat gacatgttct tgtattttcg agtcaaatgt
     4741 acgaactgta cctttacaaa atgcttgatc aggaataacg ctatctgtgg tgcctgcttg
     4801 aatcattcca aatgaaagta cagcttgttt aactggatcg attgtacgtg aaattatttt
     4861 ttgtgcactt aaaatgaact ctgccatgat tactattggg tcaatggttt catgaggttt
     4921 ggcaccatga ccgccacgac ctttaattgt gacactaaat tcatctggag aggccatgat
     4981 tgcccccgca cgtgaatgaa tagttccagt aggataacca ctccataaat gtgtaccgta
     5041 aattctatct acattttcca gacatccagc atctatcatt tcttgagaac cacctggcat
     5101 aatttcttca ccgtattgaa atattaatac gacatttcca tttaataaat gtttatgttc
     5161 atctaaaatc tctgctacag taagtaaaat tgctgtatga ccatcatgcc cacacgcatg
     5221 catacatcct ggatttttag acttataagg cacatcgttt aattcctcga caggtaacgc
     5281 atcaaagtca gctcttaatg caatggtagg tcctgtgccc aaacctttaa atgtggcttt
     5341 gataccattg cggccgatag gagtttcaat atcacaagat aactggttta attggttaac
     5401 aatataatca tgtgtttgaa attcttcaaa agataactca ggatattggt gtaaataacg
     5461 tctgagttga attgttttat tttctttatt atttgctagt tgaaaccaat ctaacaccct
     5521 aatcactact ttctaaaata ttgtttatag tataacattt tatgaaatta tcgtactaaa
     5581 tgattgcttt gagatatttt atctatgaat gataaggctt tcaagttaag tggaattact
     5641 gtatgataaa ggtattacca aacaatactt aagggggatt atatactgtg gttcaatcat
     5701 tacatgagtt tttagaagaa aatataaatt atctaaaaga aaatggtttg tataatgaaa
     5761 tagatacaat tgaaggtaca aacggaccag aaatcaaaat caatgggaaa tcatacatta
     5821 acttatcttc aaataattat ttaggactag caacaaatga agatttgaaa tcagctgcaa
     5881 aagcagctat tgatacacat ggtgtaggtg caggcgctgt tcgtacaatc aatggtacat
     5941 tagatttaca cgacgaatta gaggaaacac tagcaaaatt taaaggaaca gaagctgcaa
     6001 tagcttatca atcaggattt aattgtaata tggctgctat ttcggctgtt atgaataaaa
     6061 atgatgctat tttatcagat gagcttaatc atgcatcaat tattgatgga tgtcgcttat
     6121 caaaagctaa aattattcga gttaaccatt cagacatgga tgatttacgt gcgaaagcaa
     6181 aagaagcagt tgaatcaggt caatacaata aagtgatgta tatcactgat ggcgttttta
     6241 gtatggatgg tgatgtggct aaattacctg aaattgtaga aattgcagaa gaatttggtt
     6301 tattaactta tgttgacgac gctcatggtt caggtgttat gggcaaaggc gctggtacgg
     6361 ttaaacattt tggtttacaa gataaaatcg atttccaaat tggtacgctt tctaaagcaa
     6421 ttggtgtcgt tggcggttat gtagcaggta caaaagagtt aatagattgg ttaaaagcac
     6481 aatcacgacc attcttattc tctacatcat tagcacctgg ggatactaaa gcaatcactg
     6541 aagcagttaa aaagttaatg gcttcaactg aattacatga taaattatgg gacaatgcgc
     6601 aatatttaaa aaatggattg tcaaaattag gatatgatac aggtgagtca gaaactccaa
     6661 ttacaccagt aattattggt gatgaaaaaa caactcaaga atttagtaag cgtttgaaag
     6721 acgaaggtgt ctatgtgaaa tctatcgttt tcccaacagt gccaagaggt acaggacgtg
     6781 taagaaatat gcctacagct gcacatacaa aagacatgtt ggatgaagca attgtggctt
     6841 atgaaaaagt aggaaaagaa atgaatttaa tttaatatat atatttattc ccacggcaaa
     6901 tatcgtcgtg ggcttttttt aaaatgctta gtttattaac agtaagtggt tataacaatg
     6961 tttagtgtta tcaaccatta aagtttgaat tttaaaagca tcttgtagaa tttagttgta
     7021 tttttacaaa gaaattcatt ttgattattt ttgataatga gcattttaat agtaatacat
     7081 gtttatggtg tgtagtatat gtctatacta gtagtaacta tatagagaaa gtaggaataa
     7141 actatgtcac aagatgtaaa tgaattaagt aagcaaccaa cgccagataa agcagaagat
     7201 aacgcatttt tcccatcacc atattccctt agtcaatata cagcacctat aacagatttt
     7261 gatggtgttg aacacaaagg tgcctatata gatggtaaat ggaaagtatt gatgattgct
     7321 gctgaagaga gatatgtatt attggaaaat ggaaaaatgt tctctacggg taatcatcct
     7381 gttgaaatgt tattaccttt acatcattta atggaagcag gttttgacgt tgatgttgcg
     7441 acattatctg gttatccagc taaattagaa ttatgggcta tgccaactga agacgaggca
     7501 gttataagca cgtataataa attgaaagaa aaattaaaac agccaaaaaa attagcagat
     7561 gtgattaaaa atgaattagg acctgattca gactatttat ctgtctttat cccaggcgga
     7621 catgctgcag ttgttggtat ttctgaaagt gaggacgttc aacaaacatt agattgggca
     7681 ttagaaaatg accgctttat agttacatta tgtcatggac cagcagcact actttcagca
     7741 gggcttaaca gagaaaaatc tccattagaa ggatactctg tttgtgtctt ccctgactca
     7801 ttagatgaag gtgcaaatat tgaaataggt tatttacctg gacgcttgaa atggctagtt
     7861 gctgatttat taactaaaca aggtttaaaa gtagttaacg acgatatgac aggaagaacg
     7921 ttaaaagatc gtaaattatt aacaggtgac agtcctttag cttcaaatga attaggaaaa
     7981 ttagcagtta atgaaatgtt aaatgcaata caaaacaaat aattaaatat taattagagg
     8041 agccccatat gtaaatgtat aagggctctt tttttggcaa aatttaagtg atacttgtaa
     8101 aatagaacct attatgagta tgatttaaga aaacgcttgc aaagataata accgcaacta
     8161 gcgatatgga ggaaacatga tgtcttatag cattggaatt gattatggaa ctgcttcagg
     8221 ccgtgtgttt ttaattaata caactaacgg tcaagtagta tcaaaatttg tgaaaccata
     8281 tacacatggt gtcattgaga gtgaattaaa tggtttaaaa ataccacata catatgcact
     8341 tcaaaatagt aatgattatt tagaaattat ggaagaagga atatcatata tagtacgtga
     8401 atcaaaaata gatccagtca atatagtagg tattggtata gactttactt catctactat
     8461 tatttttact gacgaaaacc ttaacccggt acataattta aaacaattta aaaacaatcc
     8521 acatgcgtat gtgaaacttt ggaaacatca tggtgcatat aaagaagcag agaaattata
     8581 tcaaactgct attgaaaata ataataagtg gttaggccat tatggatata atgttagtag
     8641 tgaatggatg attcccaaaa taatggaagt catgaatcga gcaccagaaa ttatggaaaa
     8701 aacggcttat attatggaag cgggcgattg gattgtaaat aaattaacta ataaaaatgt
     8761 acggtcgaat tgtggattag gtttcaaagc attttgggaa gaagaaacag ggtttcatta
     8821 tgatttgttt gataaaatag accccaaatt atcaaaagta attcaagata aagtatctgc
     8881 accggttgtt aatattggtg aagcagtagg gaaactggat gataaaatgg cacagaaatt
     8941 aggattatca aaagacacta tggtaagtcc ttttattatt gatgcccatg ctagtttatt
     9001 aggtattggg tctgaaaaag ataaagaaat gactatggtg atgggaacaa gcacatgcca
     9061 tcttatgtta aatgaaaagc aacatcaagt gccaggtata tcaggttctg taaaaggagc
     9121 aattattcca gaattatttg cttatgaagc gggacaatca gcagtaggtg atttgtttga
     9181 gtatgtcgct aagcaagcac caaagtcata tgtagatgaa gcagaaaaca gaaatatgac
     9241 tgtatttgaa ttaatgaatg aaaagataaa acatcaaata ccaggtgaaa gtgggctcat
     9301 tgctcttgat tggcataatg gaaatcgaag tgtattaagt gatagcaatt taacaggttg
     9361 tatctttgga ttaactttac aaactaagca tgaggatatt tatagagcat atttagaagc
     9421 tacagcattt ggtactaaga tgattatgca acagtatcaa gattggcata tggaagtaga
     9481 aaaggtattt gcatgtggcg gtatacctaa aaagaatgct gttatgatgg atatctatgc
     9541 gaatgtactg aataaaaaac taattgttat ggatagtgag tatgcaccag caataggcgc
     9601 agcaatatta ggtgcagtca gtggtggcgc acataattca attaatgatg cagttgatgc
     9661 tatgaaagag ccaattttat acgaaattaa tccagaagcg gaaaaagtac aaaggtatga
     9721 aacattattt aaagcttata aggctttaca tgatatccat ggttataaaa aagctaatat
     9781 aatgaaagat attcagagtt taagagttga gggataaaaa atttaatttg acattaatta
     9841 gtaatattaa a
//
LOCUS       DAPIDM010000097         9832 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000097 DAPIDM010000000
VERSION     DAPIDM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.097"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(148..2604)
                     /locus_tag="RJE42_001938"
     CDS             complement(148..2604)
                     /locus_tag="RJE42_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDV5877350.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(2618..3625)
                     /locus_tag="RJE42_001939"
     CDS             complement(2618..3625)
                     /locus_tag="RJE42_001939"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDV5877351.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(3615..4181)
                     /locus_tag="RJE42_001940"
     CDS             complement(3615..4181)
                     /locus_tag="RJE42_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDV5877352.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLVE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(4200..4661)
                     /locus_tag="RJE42_001941"
     CDS             complement(4200..4661)
                     /locus_tag="RJE42_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDV5877353.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4819..6033
                     /locus_tag="RJE42_001942"
     CDS             4819..6033
                     /locus_tag="RJE42_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV5877354.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSSI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(6239..6799)
                     /gene="pdxT"
                     /locus_tag="RJE42_001943"
     CDS             complement(6239..6799)
                     /gene="pdxT"
                     /locus_tag="RJE42_001943"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDV5877355.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(6803..7690)
                     /gene="pdxS"
                     /locus_tag="RJE42_001944"
     CDS             complement(6803..7690)
                     /gene="pdxS"
                     /locus_tag="RJE42_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDV5877356.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            7794..9176
                     /locus_tag="RJE42_001945"
     CDS             7794..9176
                     /locus_tag="RJE42_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV5877357.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYNPKASDESYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITIKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         2980 a         1866 c         1394 g         3592 t
ORIGIN      
        1 cattaacttg tttcatataa ttcttaacta tcctttgatg attattttat tagattgttt
       61 cgttggtgga tactttcacg aatttctata gttcaatgct actaaaaaac agcccaaaac
      121 tttaatttgt tttggactgt tttaaaatta tgcttgcgat ggtgttttag tttctgaagt
      181 ttgttcagca atgtcatatt taaactcttt accatcatga tctactgtaa ctttcttacc
      241 ttcaatttga ttaccatcta atattaattc acttaaatta tcttcgatag ttttttgtat
      301 cgctctaatt aatggtcttg caccatattc tggatcatat ccttcttctg cgattttatc
      361 tttcgcttta tcagtaacaa taatatttat gttttgttcg gataatcgat ttgttaattt
      421 attaaccatc atagttacaa tttcttttaa ttcttctttt gttagtttat ggaatacaat
      481 gatatcatct acacggttta aaaattctgg acggaatgaa ttttttaatt cttttaacat
      541 cgtttttcga attgtttcat aatcttgtcc atcacttgaa ccaccgaatc cagcaaatcg
      601 ttgatcttgt aattcttgtg ctccaacgtt tgatgtcatt atgataattg tatttctgaa
      661 atcaactgta cgtccttttg tatctgtcaa atgtccgtca tctaaaactt gtaatagaat
      721 attaaataca tctggatgtg ctttttcaat ttcatcaaat aaaattacag aatatggttt
      781 acgtctaact ttttcagtta attgtccacc atcatcatga ccaacatatc ctggaggagc
      841 accaactaat cggctcactg cgtgtttttc cataaattca ctcatgtcta cacggatcat
      901 cgcatcatca tcgccaaaca ttgattcagc taaagctcta gctaattcag ttttaccaac
      961 accagttggt ccaaggaaga taaagctacc aattggtcgt ttaggatctt ttaaccctgc
     1021 acgggcacgt ctaaccgctt tactgattga attaacagca tctttttgcc caataactct
     1081 ctcatgtaat gtatcttcta gactaagaag tttttcagat tctgtttcgt tgattttagt
     1141 taatgggata cctgtccatc ctgcaatgac ttcagcaata tcttcttctg acaatgaagt
     1201 tgacatgcca ttttgtgcgt tcttccattc atttttagct tcttcatatt gcttttcaag
     1261 ttttgtttgt ttatcacgca gattagcagc attttcaaac tcttgagcat gcactgccgc
     1321 atctttttca tttttaactt tttcaatttc ttgttcaatt tcttttaaat tattaggtgt
     1381 cgtatgactc ttaagtctta ctttagaact tgcttcgtca attaaatcaa ttgctttatc
     1441 tggtaagaaa cgatctgaaa cgtatctgtt acttaattta acagctgctt caatagcttc
     1501 gtctgaaata ttaatacgat ggtgtgcttc gtaacgatct cttaatcctt ttaaaatagc
     1561 aactgtatct actactgaag gttcatcaac ttgtacaggt tggaaacgac gttctaaagc
     1621 cgcgtctttt tcaatatttt tgcgatattc atctaatgta gtagcaccaa tgcattgtaa
     1681 ttcaccacgt gctaatgccg gtttcaaaat attcgaagca tcgatagcac cttcagcacc
     1741 accagcacca actaaagtat gcaactcatc aataaatagg atgacattac ctgcttgttg
     1801 gatttcttcc ataacctttt tcagacgctc ttcaaattca ccacgatatt tagtacctgc
     1861 aactactgtt cccatatcta aagacataac acgcttatct tttaatgtct ctggtacctc
     1921 attattcact atggcttgcg ctaaaccttc agcaatagca gttttaccaa cacctggctc
     1981 tccaataagc acaggattgt ttttcgtacg tctacttaat acttcaatta cacgtgtaat
     2041 ttctttatca cgtcctataa caggatctaa tgtaccgtct ttggcaatga ctgttaagtc
     2101 acgagctaaa ctatctaaag ttggagtatt atttgactta ctagcttgtg catttttatt
     2161 actcatttca gggtttccta aagctttcac aacttgtgca cgtgctttag taatatttaa
     2221 atctagattt gcaaaaactc ttgctgcaac accttcattt tcacgaatca agcctaataa
     2281 aatatgttcc gttccaacaa aattgtgatg taattttcta gcttcatcca tcgataattc
     2341 aatgactttt ttagctctag gtgtataatg caatgtacca acatgatctt gaccatgtcc
     2401 gattaatttt tcaacttctt caattacttt atcttcagtg atattaaaac tttctaatac
     2461 ttttgcagca attccttcag gttctttcat taaccccaat aataggtgtt ctgttcctat
     2521 atttgaatga tttaaacgaa ttgcttcttc ttgtgcatgt gctaatacgc gctgtgcacg
     2581 ttcagttaat ctaccaaata acataaataa tgacctccta ctttatatgt tctcttagta
     2641 tatctgctcg tttttctttt acagatttgt catcttcttc atctaataaa aatggtgact
     2701 gtatagctac catcaattca ttaaatttaa agttttgtaa ttcaatgtaa tttaaatcta
     2761 taccaagttt aacttcgctt aatctataag aagcctcttc catagttatc attctacagt
     2821 tttgtaaaat acctagcgag cgaaaaacac ggtcttgtgt ttctaattga ttataagtgt
     2881 ctaacttttg tcgtatttgt ttttcttcat gaatgatttg attaacaact tctgttaatg
     2941 tttctatgat ttctaactca gatttaccaa gtgtaagttg gttggatact tgataagtat
     3001 gtccataaac ttgcgaacct tcaccgtaaa tacctctgat tgtatatcca aaacgattaa
     3061 tggtttgagc aatccgtgtc attcttttca taatagatag acctggtaaa tgtagcatca
     3121 cgcttgctct cataccagta cctatattgg taggacatgt agttaaataa ccaagttgtt
     3181 catcataact tatatcaagg cttcgatcta attcatcatc aattgatgaa gcttgattat
     3241 ataaagcctg taatgttgtg tcagttccca tagcttgaat acgaatatgg tcctcttcat
     3301 ttatcatgac acttaaagat tcatcatcat tcactaatac tgcggctgct ggttgtttta
     3361 ttagttcagg actaatcaaa tgctttgcaa ccattttcat tttactttgt tgatccattt
     3421 gatcaagacg cattaattca aaatttggca aggcatcttg tacctcattt ataactctaa
     3481 atccatcatt ttcagtagca tacattagtg gatgcacatg attttctaaa ttacgcgcta
     3541 accgaattct agaagacata acaattggtg tttcttcatt acttttcatc cattggctga
     3601 tattatcatg aatattatgc gtcatcatgt tgcacctcac tctcagcttt tagtgcttta
     3661 atttcatctc taacaatggc tgcttcctca aaatcttgga tttcaacaag ttttttcaaa
     3721 tattcattct tttcttcgat ttttcgcttt aaagctatct ttttatgtga agaatgtggt
     3781 gtctttccaa cgtgctcaaa ttgtccacct tgaactctgc ggacgatatc aatgatgtca
     3841 tctttaaatg ttgcataaca attagcacac ccaaatttac caacatgtgc aatatctttc
     3901 aaagtcatgt gacatgatgg gcaacgtttt tcttctttaa acgctacttc ttgaaaattg
     3961 atgccatgtt tcgtagctaa atgttgtaaa atttgcttaa caacaaatgc ttcttcgaaa
     4021 ttatcttgat gttcttgata ttcaggttgt tcattagctt gattccacgg atggtgcccc
     4081 tcagcacaag tttgacacac cattttttct tctgttttat ttttacttgt aactttaact
     4141 tttaattccg cttcattaag ttgacaattt tcacaaagca cttcaacacc tcatttcatt
     4201 tagtaataat ttataactgg taacaaacgt tttaaaatat ttgctctaat aatatctcta
     4261 gaaaccatat ccattgatag cgtttctcta tcaataactg cttgaatcat tttagcttca
     4321 cgttcattta ttaacatttt atctaaaagc ccatcaataa tataataagc ttgttgttga
     4381 gaaatagaag gtccaatcag ctgaagcaaa tgattaatat aacctgttgc atctttattt
     4441 tcaattttag tgattcggat gtaaccacca ccaccacgtt tactttcgat ttcataacca
     4501 tgttcattag tgaatcgtgt tttgattaca tagtttaact gtgatggtac gcaatcaaaa
     4561 cgctgtgcga tattcgctct ctgaatttca acgacatcct cattcgactc ttcaaataaa
     4621 cgtttgatgt attgttctat gatgtcagac atattgtgca tgtatatcac ccctttttga
     4681 ccttctttga ctatattata tgacctattt tgaccttttt caattaattt gtttttatat
     4741 tttcaaaaat ttatgatgtt agcccttcca ttttattgta tgatagattg tagaaaaaac
     4801 taaaggaatg agaaatatat gcatattcta attgggatta tcggaattat cttcttttta
     4861 gcacttgcgt ttctatttag ctcagacagg aaaaatattc gctggaaata tgtaggaatt
     4921 ttacttgcaa ttcagcttat ctttgcattt atcttgctta aaactacaat gggaatcaag
     4981 gttattggtg gtatttcgaa cggttttaac tacttattat taaaagctgc agatggtgtt
     5041 aactttgtat tcggtggtat tcaatatatc gatcctaagc aaccaccatt cttcttcagt
     5101 gtattattac ctatcgtatt tatttcagcg attatcggta tccttcaata cacaaagatt
     5161 ttacctttaa tcattaatgt tttaggattc ttaatttcta aaattaatgg tatgggacgt
     5221 ttagaatcat acaatgcagt tgctgcagca attttgggac agtctgaagt atttatttca
     5281 attaaaaaac aattaccata catacctaag caacgtttat acacattaac agcttcagca
     5341 atgtcgactg tttctgcttc aattatcggt gcatacttta ccttaattga acctaaatat
     5401 gttgtaacgg ctgttgtctt aaacttattt ggtggtttca tcatcgcttc aattattaac
     5461 ccttataaag ttaatgaaga agatgacaaa ttattagttg aagaaactga agaaagacaa
     5521 caatcattct tcgaaatgct aggagaatat attttagatg gatttaaagt agctgtcatt
     5581 gtaggcgcaa tgttaatcgg atacattgcc attatcgctc tattaaatgg aattgtaagc
     5641 agtatcttta gtacagtttc aggtggcgct atttcttggg atttccaaac attaattggt
     5701 tttgtctttg caccatttgc attcttagtc ggtgtgccat ggcaggatgc agttcaagcc
     5761 ggttcagtta tggcgacaaa actattatct aacgaatttg tagcaatgca agcacttggt
     5821 aaacttagtg atttatctga acacgcaaaa ggtgttacat ctgtattctt agtatcattt
     5881 gctaacttta gctctattgg tattatttca ggtgcgatta aatcacttaa tgacaaaaaa
     5941 ggagatacag ttgcccgctt tggtttgaaa ctattattcg gtgcaacact agtatcattc
     6001 atttcagctg caatcgctgg attctttatt taacaaatac atcaacatcg aattaacccc
     6061 ttcaaaattt tgactattct cagtctcttt gaagggtttt ttcttatgta tatcttattt
     6121 tttatcattt ttaattttga cataaaaaat aactcctaca aagctaagtt tgaaggagat
     6181 attgtcttct caacaaatct ataaatatca ataattcgtt gatttagcat acattaagct
     6241 atgctttttt tacaatatga ttaataaagt aatccgttac tctatagtca tctgttaatt
     6301 caggatggaa tgatacgcct aaatatttac cttgctggac agctacaatt ttctcattaa
     6361 ccttacatag gatatctacg ccttgaccta ctttttcaat atgtggcgct cttataaaga
     6421 caccttcaat atctgtagcg atacctttaa tatctaattc tgtttcaaag ctgtcaactt
     6481 gtctaccgaa tgagtttcgt tgtacagtaa tattcaactt gttaaggtat ccttcttcac
     6541 caactatatc ttgcgctaga actattaatc ctgcgcatgt accaaacata ggtaaagttg
     6601 aattttgtaa agcctcttta aatccatata aattcattaa tcgacgtaac gttgtagact
     6661 cgccaccagg taatattaag ccctcgattt cttctaattg ttcaactttt ttaactgcaa
     6721 taccttcatg accacttagt tcaatatgtc taatatgttc acgtactgca ccttgtaatg
     6781 ctaatacacc tattttcata tcttaccaac cacgctcttg catacgttct tctaatgata
     6841 attgattgat atctaaacct ttcatagcag tgccaagttc acttgctaat cttccaatta
     6901 gttcatagtc ttggtaatgt gttgttgctt gaacaattgc tttagcaaat ttttctggat
     6961 cttctgattt aaaaatacct gatccaacga atacaccatc ggcacctaat tccatcatta
     7021 aagcagcatc ttgaggagtc gcaacaccac cagctgcaaa gttaactacc ggtaaacgac
     7081 cattgtcttt aatttgtttt aaaatttcat aaggcgcacc gatatctttc gcgaaagtca
     7141 taatctcatc atcattcatt acagtcaatc gactaacttc tgaattaact tgtctcatat
     7201 gtcttacagc ttcaacaata ttacctgtac ctggttcacc tttagtacgt aacatagcag
     7261 caccttcacc aattctacgc gcagcttcac ctaaattacg acatccacat acaaatggta
     7321 ctgtaaattg atcttttctt aagtgatatt cctcatctgc tggtgttaac acttctgatt
     7381 catcaatata gtcaacaccc atcgcctcta atactcttgc ttcagtgata tgaccaatac
     7441 gtgctttagc catgactgga atagaaacag cattcattac ttcttctaca attttagggt
     7501 ttgccatacg tgcaacgcca ccagcagctc taatatcaga aggtactcgt tctaatgcca
     7561 taactgctac cgcgccagct tcttctgcaa ttcttgcttg ctcagcatta acgacatcca
     7621 taataacgcc gcctttttgc atttcagcca tacctctttt gactctgtct gatccaataa
     7681 ttttactcat tatacataaa accccttttc aagttgatta aatacattgt aatgtataaa
     7741 ctgatattta aaaaggtaca aaataagata aatttaggag gtcagttata aaattgaaaa
     7801 acactttata ccatcaactt tatgaaaaat taaaaaaaca aataattgaa ggtcaattta
     7861 aagaaggcga taaattttat tctaaaagac aattaagcaa acatttatct atcagccaaa
     7921 caaccgtaga gcatgcatat caacttctat tagacgaagg ttacatttat tcaagacctc
     7981 gctctggata ttttgtgtcc gaaattgaat ctttaacaat tttaaacaat caacctatcc
     8041 cttctctttt tgatgatgat tcatataatc caaaagcgag tgatgaatct tatgattatg
     8101 cttttaattt ggatgaaata gatacgaaac atttccctat agaacttttc agaaagtatt
     8161 ccaaagatct ttatgataca aatcatttaa atcaactacg tagaggacat tttcaaggag
     8221 aattacattt aagatttcaa ctggcatttt atttatttac caatcgtggt gtaatttgtg
     8281 atcctaatca aattataatt ggctcatcta ccgaacaatt agtaaatcaa ttagtcgatt
     8341 tactttatac ttcaacgttt attatcgaaa aacctagtta tccaccaatc aaaaatattt
     8401 tagataaaaa acaagttgaa tatgaacaaa ttgaagttga agataatgga ataaacgtag
     8461 atgaagttat taaatcacaa aaaaatatag tttatataac gccttcccat caatttccaa
     8521 ctggatatgt aatggactta aaaaagcgaa ctcaattaat acaatgggct caagaaaaag
     8581 aagaacgatt tattatagaa gatgattatg attccgagtt tagatatttc ggtaaaccca
     8641 ttccagcaat tcaaggtcta tattcaagag gagaaaaagt gatttatatc agtacatttt
     8701 caaagtctat ttttcctagc tgtcgtgttg catatatggt gttaccctat tctataatga
     8761 aaaaatatca ttctcaaaat cacattgaag gcaacactgt acctgttcat atgcaaaacc
     8821 ttattgcaac gtttatatct tccggtggtt ttgaaaggca tttaaataaa atgagacgaa
     8881 tttatcgtag aaaactaacc tatattttaa aaagattaaa accatacaaa gagcaacttg
     8941 atattcaagg tgctgaaaca ggtatgcatt ttacaattac aataaaaaat ggattaacat
     9001 tacaagagtg tcttgataga gcaaataaag taaagttaaa attacaagtt tataattttg
     9061 atgatgacca agactataag aaaaccccta aatttatatt aggctttggc ggtatagacg
     9121 atgatgcttt aaaaccgcat gtagacgctc taataaaatc attaattata aagtaacact
     9181 tctttgacaa aagttagtgt tacttatgca atagcttgtc tattgtataa taattaattt
     9241 cctttttgta tttcgattta aaagatatta aacataaaac ctaaaaacag cagtaagatg
     9301 atttatgatt aaaaactatc ttactgctgt tcacttttta taatacttct gaatgtcttc
     9361 acttatactt ctagtcacag atttagacaa tcgaaaatgc acattattaa attatcattt
     9421 ccaaaactca atgcggctca tcgcatccat ttttgcctgg caacgttcta ctctagcgga
     9481 acgtaagttg gctaccatcg tcgctaagaa cctttcttga cttgtgacaa tcgcttgctt
     9541 ctttcctctc cttcggctct cgcttactca tttagctcta ctaaactcgt tacgctcttt
     9601 tctcgtttcg tcagattcaa acgttttcac ttcgtcaagc catttttctt tgtgttaact
     9661 tttattttga cgttttagac ataaaaaaaa gagatcttgc ggtctcaatg cggctcatcg
     9721 catccatttt tgcctggcaa cgttctactc tagcggaacg taagttggct accatcgtcg
     9781 ctaagaacct ttcttgactt gtgacaatcg cttgcttctt tcctcttctt cg
//
LOCUS       DAPIDM010000098         9767 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000098 DAPIDM010000000
VERSION     DAPIDM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.098"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(380..1198)
                     /locus_tag="RJE42_001946"
     CDS             complement(380..1198)
                     /locus_tag="RJE42_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5877358.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSK
                     TVDKIKSNGDNAKAYTVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKTFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            1432..2970
                     /locus_tag="RJE42_001947"
     CDS             1432..2970
                     /locus_tag="RJE42_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDV5877359.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVIVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(3082..3504)
                     /locus_tag="RJE42_001948"
     CDS             complement(3082..3504)
                     /locus_tag="RJE42_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV5877360.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(3976..4374)
                     /locus_tag="RJE42_001949"
     CDS             complement(3976..4374)
                     /locus_tag="RJE42_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877361.1"
                     /translation="MNKKHVFIIIGVILCIRIVATVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKTGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGDMIESEKLSELLKPAQELKSQKKSKKN"
     gene            complement(4401..4811)
                     /locus_tag="RJE42_001950"
     CDS             complement(4401..4811)
                     /locus_tag="RJE42_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877362.1"
                     /translation="MSKKHVFIIIGVILCICIVVTAMHLKMKYDEKEKRKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNLETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPDDIKKELNKK"
     gene            complement(4829..5248)
                     /locus_tag="RJE42_001951"
                     /pseudo
     CDS             complement(4829..5248)
                     /locus_tag="RJE42_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            complement(5266..5649)
                     /locus_tag="RJE42_001952"
     CDS             complement(5266..5649)
                     /locus_tag="RJE42_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877363.1"
                     /translation="MLIILIIIVCVYLKIKIDEKEKQKAIYYKEQQERITLYLKHNTE
                     EPNTIKSVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQFGGDMVESERLS
                     ELLKPAHERKSPDDIKKELNKKKNH"
     gene            5716..6924
                     /locus_tag="RJE42_001953"
     CDS             5716..6924
                     /locus_tag="RJE42_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877364.1"
                     /translation="MNSIYDKDTTYRALAKEAYHLEPDQEKIFVPYSKEKKIEFRIVA
                     FRQNKFNGLKMAGLSPVEKGAKLNTDELYIVYAGTDPDLLADFGSDSIEDLKVGMAKF
                     GGGLRIPPTKKERAIYGNLNKKMIDYDDGISGISQNIMTNPISQSKESYIFTGALINK
                     QKPKHVYGAAHSLGGGLALLNGVMYNFDGVRAFSAPNTFDLLPDEVKANYRSEKYDGK
                     FINYLHRSDIIGNSDLFAQRIGTQIYGKDVGRFSLLNPILGHGMDTFEFKGDNVRIKM
                     DTEEMSKIASDLKASCEFVNDAIKSYQKYMDDTKAAAKRIERKYTKKIRTGNYKHIKP
                     SDIEDYMEELSVSGKYEFYNDTAFETVMSELNRIRKNVESFADKLKHAGEKMESRDKE
                     IGELYKIFER"
     gene            6930..7244
                     /locus_tag="RJE42_001954"
     CDS             6930..7244
                     /locus_tag="RJE42_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877365.1"
                     /translation="MGFLDDLAESIEEQSDLSEFKQAMKSELQTKVEEAKAKIESIDE
                     DIWDWQTTVNNKRDAIEEYCEGKTAEHAIERLMQDKQDIAGMRWTAEDAEKSCKVGSW
                     FW"
     gene            complement(7533..9070)
                     /locus_tag="RJE42_001955"
                     /pseudo
     CDS             complement(7533..9070)
                     /locus_tag="RJE42_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611580.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9121..9540)
                     /locus_tag="RJE42_001956"
     CDS             complement(9121..9540)
                     /locus_tag="RJE42_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877366.1"
                     /translation="MPKKTIFIIIGVILCISIVTGGIYLKMKHDEKEKQKEIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTNLKTGPMGDIEIYGYINSKKDYDFIAYNSPEENYQ
                     FTGGLTGSNEVLGKLKNAENLKSPEKIKEELNNKKDH"
BASE COUNT         3070 a         1576 c         1503 g         3618 t
ORIGIN      
        1 aaagtccaat aataataaac taacggaatt agtttctaac tgattatctc caattttatc
       61 tgcaatctct cttatagctt gatatagcaa tctaaaactt tctttttctt ttaacataaa
      121 attctcctta atttattaga atagacaaaa tacagcttat tttaaagata ctccaaaagt
      181 ttgaactcga gattctgata aattagatgc acgacctaca atacgttaaa acaagcttta
      241 agcaatacac ttcaaatgaa caatatgtga ttttcaaaga ttaattaatc tccgatttaa
      301 atgtctgtca ttgcgacaat atacatacat tgcctacttc atgccaaatt attttccact
      361 ggatatcgat tttaaataat tattccaatg tccgcttcca cgaatcatca ctcaacatct
      421 cgccaggcca tgtgtaagcc ataacaccac cgtcaatgcg aattgtttca ccagtaataa
      481 atgaactctc atcagatgct aaaaagacaa cgagcttgcc tacttcttct ggtttaccta
      541 aacgtcccag cggtgtcatc catttctgat tttctctgaa tgttttgcct tccttatctt
      601 cgctagtacc tgtcagttta tctactaacg gggtttcaat cgtacctggt gcaatcgcat
      661 tagctcgaat gccatctcta ccatattcga ttgcaatgga tttcgtaaaa ttaatcactg
      721 cccctttcgc agcattatat cctgagcgat acaaatctgc tgcctgtcct gaaaatgaag
      781 atgtgttaac aatagaacca cctttagtca tcattaaagg taacatcatt ttcgtcatca
      841 aaaatgttcc acgcatatct acattcataa tcttgtcata tacatctgtt ggatactcat
      901 gaattctacc agcagcattg tcaacgcctg cattattaaa caacacatcg attcttccaa
      961 actgttcctt tatgtcagac acaaagtcta ccacttgttg ttcatctgaa atatccacag
     1021 tatacgcctt cgcattgtca ccattacttt taattttatc gacagtcttc gataccgctt
     1081 cagctatgtc taccgccaat acatacgcac cttcttgagc taaagcgatt gcagaagctt
     1141 gtccgatacc tgtacttgct cctgttacga ctgcaacttt attttccaaa cgtttcattc
     1201 agtcactcct atacaacata tcttaaactt acagtcctta tttcccactg ttcaatgctt
     1261 cataaaccta atagataagt ctccaggcag tcttagctgc tctctgctat acaatttcta
     1321 ttgaataact caaaatattt aataatatta gatttttcat tttctgaaaa ttgttacttt
     1381 tgagagtatt ttttgttaca ctttaaatat ttaacaaagg gggaatcatt gatgacatca
     1441 aaacatcaac aaaaggggtc tatcgtcaat cgtttcttga atagtgttga aaaaattgga
     1501 aataaattgc cagatcctag cgttttattc tttttaatgt gtgtgggctt agccattatg
     1561 acgtgggtta tctcattatt taatgtatct gttaagcatc caggtacgca tcaaaccatt
     1621 tatattaaaa atataattag ccatgatgga tttacgatga taatgaatga tacgattaaa
     1681 aacttctcag agttcccagc attaggctta gtactagcag tgatgattgg tattggtgtt
     1741 gcagagaaaa caggatactt cgacaagtta atgatttctg ttgtgaatcg cgcaccacgt
     1801 ttcttaattt taccgactat tatattaatt ggtattttag gtagtacagc cggcgatgct
     1861 gctacaatta tcttgccgcc gctcgcagcg atgcttttta ttaaaattgg ctatcaccct
     1921 atcgctggac taacaatggc atatgcttcc gctgtaggtg gctttgccgc aaatatagtt
     1981 gttggtatgc aagatgcttt ggtctattca tttacagaac cagcaacacg tatcgtttca
     2041 gattctatta aaacaaacgt tgctatgaac tggtacttta tcgcagcgag tgtcattgta
     2101 ttacttccta cgatattgct agttacaact aaactgatta ttcctaggtt aggtcaatat
     2161 gatgatcgtt taatgcatga tgatcatgaa gaaacgtctt cacatattac tgacaaagaa
     2221 gcacatgctt taaaatgggc aaatatcagt ttcatagtca caattatttt attaattatt
     2281 acagccattc ctgaacatag ctttttaaga aatgctaaaa caggcagctt actagacgat
     2341 gcgccattaa ttaacggcgt cggattaatt attcttgttg tatttttagt acctggatta
     2401 gtttatggga ttttaagcaa agaaattaaa aatacaaaag acttaggtaa aatgttcggc
     2461 gatgctgtag gatcaatggg aacatttatc gttattgtgt tttttgcagc acagctactt
     2521 gcgtatttaa agtggagtaa tttaggaatt atcgctgctg ttaaaggtgc caaattatta
     2581 gaacatcaaa acggtattgt attgatacta ggtattattg tactgagtgc aatggtcaac
     2641 atgttaatcg gaagcgcatc ggctaaatgg ggtattttag gaccgatatt cgtaccaatg
     2701 ttaatcctta tcggctttca tcccgcattt acacaagtta tttaccgtgt aggagattca
     2761 attaccaatc caattacacc gatgatgccg tacttacctt tattattaac ttatgcacaa
     2821 aaatacgata aacgcatgaa gttaggtgct ttactttcta gtttaatgcc gtattcaatc
     2881 gcactaagta ttgtgtggac agtatttgtc atcatttggt tcttattagg tatccctgtg
     2941 ggaccaggtg gaccgatatt cgttaaatag cagtattcaa agggataaat taaaatgcca
     3001 ctatacaaga ttcgacctgt atagtggcat gatttgttat gatatagcgt tcgagatgaa
     3061 actctgcatt tatatgaaca attatagtct atcactaata atatttaatc tgttccaacc
     3121 gttaatttgg tttacaacaa agacaagatc aataatttgt tcttcatcat aaaatgattt
     3181 tagccggtca ataatttcaa acttcttaaa gtctttgatt gaatttaaca tttctgcaaa
     3241 ttcaagcgtc actttttctt gatcagtaaa taaatctaaa tgtctaaaac tcaagacttc
     3301 atcaattttc atttgtgtta cacccaattc ctttaattct tttttatgga tatcaacaca
     3361 ataatgacaa ccgttaattt ttgaaattaa taacttaatc aatgcactaa gttcatcatt
     3421 aacgggtgat tgatgaataa acttttcacc ttggctcatt acagacatta actctccatt
     3481 ttgtttcttg taattatacg gcatttgcaa tctctccttt acattgttta ataccaatcc
     3541 agtgatacta cacttcgaac aatattggat agaatttgaa attctgtata acatcttgtc
     3601 aatctatgct ttagttaaaa catttagcgc ttatatttaa aaaatatata aaatatgaaa
     3661 acagattgaa aggatattat accttgctct accctttttt ataaaaaatc acacacattt
     3721 gagaaaattt gataatgtca ataaatcatg tcatcgccat tgttattcgg tatataatgt
     3781 ttttataatg gctaaactat tatttcattt acattaatgg ggttcgcttg tgttaatgta
     3841 ctgtaaaaaa ctggggaagt attggtgatg atgaagtgat ttatattaat tgcgaaacac
     3901 cttcgatagc aatgacatag attattggaa gattaaatca aaaataaaga agaccctagt
     3961 ggccttcttt tttgtctaat tcttctttga ttttttctgt gattttaatt cctgtgctgg
     4021 ttttagtaat tcacttaatt tttcactttc tatcatatct ccaccaaatt gataattatg
     4081 ttctggagac ccatatgcaa caaaatcagc ttttttgttt tcattgatgt aaccttcaat
     4141 tacggcatcg cccataggtc ctgtctttaa acttgtgaaa tgcacagatt taatcgtgtt
     4201 tggttcttta gtattatgct taagatagag tgtaatacgt tcttgttgtt ctttgtagta
     4261 aatcgctttt tgtttttctt tttcatcata tttcactttt aaataaatga ctgtagcaac
     4321 tatacgtata cataatatga caccaataat tataaaaaca tgttttttgt tcattaaagt
     4381 aactcctaac tttagtgact ctacttttta tttagttctt tttttatatc atcaggtgac
     4441 tttctttcat gtgctggttt taataattta tctactcctt cactttttat catagcgcca
     4501 ccaaattgat aattatgttc tggtgtagca taagcagtaa aattatcttt tttgttttca
     4561 tttatgtaac cttcaatcac agcacttccc ataggacttg tttccaaatt tgtgaagtgt
     4621 acagatttga tcgtattcgg ttctttagtg ttatgcttga gataaagtgt aatacgcgct
     4681 tgttgttctt tataataaat tgcttttcgt ttttcttttt cgtcatattt catttttaaa
     4741 tgcatggccg taacaactat acatatacat aaaatgacac caataattat aaaaacatgt
     4801 tttttggaca tttaaccacc tccaactttt agtgactctt ctttttgttt agctcttttt
     4861 ttatatcatc aggcgatttt aattcctgtg ctggttttag taattcactt aatctttcac
     4921 ttttaattaa atcaccacta aattgaaagt tattttcagg tgatgcatag gcaacaaaat
     4981 catcttcttt attttcattt atgtaacctt caatcactgc acttcccata ggacttgttt
     5041 tcaaacttgt gaaatgtaca gatttgatgg tattgggttc tttggtatta tgcttgagat
     5101 aaagtctaat acgcgcttgc tgttctttat aataaactga tttttgtttt tatttttcat
     5161 catatttcat tttaaaatgt atgactgtag cgactataca tatacataaa atgacaccaa
     5221 taattataaa aacatgtttt ttggacattt aaccacctcc aaaatttagt gattcttctt
     5281 tttatttagt tcttttttta tatcatctgg tgactttctt tcatgcgctg gttttagtaa
     5341 ttcacttaat ctttcacttt ctaccatatc tccaccaaat tgataattat gttctggaga
     5401 tccatatgca acaaaatcat cttctttatt ttcattgatg taaccttcaa ttactgcatc
     5461 gcccataggt cctcttttta aacttgtgaa atgtacagat ttgatggtat tgggttcttc
     5521 ggtattatgc ttgagataaa gtgtaatgcg ctcttgttgt tctttataat aaattgcttt
     5581 ttgtttttct ttttcatcaa ttttaatttt taaatagaca cacacaataa taattaatat
     5641 aattaacata atactaataa ttattgtttt tattttttcg acataaatag taaattccta
     5701 aagtgaggtg gagttttgaa tagtatatat gataaagata ctacatatcg tgcacttgca
     5761 aaagaagcat atcatttaga accggatcaa gaaaaaattt ttgtgcctta ttcgaaagag
     5821 aaaaaaattg aatttagaat tgtagccttt cgtcaaaata agtttaatgg attaaaaatg
     5881 gcgggattgt cgcctgttga aaaaggtgca aaattgaata ccgatgaatt atatatagta
     5941 tatgccggca cagaccctga tttgttagca gatttcggct ctgattcaat tgaagattta
     6001 aaggttggaa tggcaaaatt tggtggtggt ttaagaatac caccaacgaa aaaagaacgc
     6061 gcaatttatg gtaatctgaa taaaaaaatg attgattatg atgatgggat ttctggaatt
     6121 tcgcaaaata ttatgaccaa tccaatttct caaagtaaag agtcatatat ttttacaggt
     6181 gcattaatta ataaacaaaa acctaaacat gtatatggtg ctgcccatag tttgggtggc
     6241 ggtttagcat tgttaaatgg tgtgatgtat aattttgatg gtgtccgtgc tttttcagca
     6301 ccaaatacgt ttgatttgtt accagatgaa gttaaagcaa attatcgttc tgaaaagtat
     6361 gatggcaagt ttataaatta tctccatcgt tcagatataa ttggtaattc agacttgttt
     6421 gcacagcgta taggtacaca aatttatggc aaagatgtag ggcgttttag tttgttaaac
     6481 cctattttgg gtcatggtat ggatacattt gaatttaaag gtgataatgt tcgtatcaaa
     6541 atggatacag aagaaatgtc aaaaatagca agtgatttga aagcatcttg tgaatttgta
     6601 aatgatgcga ttaaatctta tcaaaagtat atggatgata ccaaagcagc tgcgaaacga
     6661 attgaaagaa aatatacaaa aaaaataaga acaggtaatt ataagcatat aaaaccatca
     6721 gacatagagg attacatgga agaattatct gtatcgggaa aatatgaatt ttataatgat
     6781 accgcatttg aaacagttat gagtgaatta aatcgaattc gaaagaatgt tgaatcattt
     6841 gcggacaaat taaaacatgc tggcgagaag atggaatcac gtgataaaga aataggtgaa
     6901 ttatataaaa tatttgagag gtaatgtata tgggtttttt agatgatctt gcggaaagca
     6961 tagaagaaca aagtgattta tctgaattta aacaagctat gaaaagtgag ttacaaacta
     7021 aagtggaaga agctaaggca aaaattgaat ctattgatga agatatttgg gattggcaaa
     7081 cgacagtaaa taataagaga gatgctatcg aagaatattg tgaaggtaaa acggctgagc
     7141 atgcaattga aagactaatg caagataagc aagatatcgc aggtatgcga tggacagcag
     7201 aagatgcaga gaaaagctgt aaagtgggta gttggttttg gtaaatgtta actcctttta
     7261 acatttttaa atgcacaatt ctagtgttat ataataaatt tagtgagcta tctacgaaga
     7321 aattagtgtt tagtgattat taataaattc tcttgaagta aacgcacaaa tgattagaac
     7381 aatcttacta cttatttcaa aaagcaaaca ctgattatta taaagcagat aattttcatt
     7441 tcaaaacata tttgatgtca atgaaatatg ttttgaatta aaataaatgg ttgtttttgt
     7501 tgctatacag aatcagccat ttttaattta attcattcat cattgaagaa atgaattaaa
     7561 tcttgatttg tttttacagc ttattctaca ctattaaatt taaaatgctt atttcctcta
     7621 aaaatttcac tttttatttt atcccataca cctttcatat gacctttatc gttacatttg
     7681 ctaacatgtg gcatactttg aatcatgcta tattcaccca atatatgttt gaatattttt
     7741 gatgtataat aacatcctcg gtcactatga aataaaatac ctttggtatt ttatactttt
     7801 tggatcgctt aattgaatgt tttgaagact aattaattat tgtttgaagg gccaagctca
     7861 taactaacaa tgtgtttggg cacctaaatc ataaatggca ctcaattaaa gcttactacc
     7921 gtctttgatt ttaaattcag ttacatctgt taaccaaact ttattcggcg tatcaatgtc
     7981 aaattatcta ttaagtctat tttctgatat gatttgtggc gttgaacgtc gataaggtgt
     8041 tcgcttttga cgtattacag cctttaaatt aagttctttc atcaaacgat aaattcgctt
     8101 tcgttttact tgtttaccta gttttaatcg aatataaata taaatacgac gatagccata
     8161 aataccttta tacttataat agattgattc tatttcttgc ttaagctgtt cattctctaa
     8221 ttcatgttca ctcggttgat gatgtaccca cttataataa ctcgctctag aaatgcctaa
     8281 aatacgacac tagtgttgga tagggtattg atgttttaac gcttcaatcg ttttgtatgc
     8341 tgcgatttgt tttctctttg aatcatctct ctttctaatg cttgaaactt ttttaaaacg
     8401 tcattttcca tttctagtaa tcgattttgt gcttttaatg cttgaatttc agcttccttt
     8461 tgctcttctg gcgtcatcat agatttgggt tttcctttgc ctctgccatc tattaaccca
     8521 tttttaccat gtgcttgata cttttgggcc cactggtata tttgaccgta tgagatatta
     8581 aagtgttctg caacttctct ataagattgt ttatgattaa agtaatattc aacaattgtg
     8641 atacgttctt caaatgtggt ttttcttgct ttcatgatat acaccctaga tttcggagat
     8701 tgattcttaa tcacttttcc tatagtatac atttttatcc aatttttgac ttgtgtcgag
     8761 ctacgaattt tgtatttgat ggcgagatct tgggttgatt gatttgaatt caaatattca
     8821 tttacaactt ttaattttag ttcctttgaa tagcgattgt attgttattg tgtaattagt
     8881 gcttcaatac catgggggga taatatttat acttaggata caaagtattg acattaatat
     8941 ctaattgtaa tgtatcgatt aggtattgaa acgatacacc tttttcatat tcttgaaata
     9001 tataaagcag tacatctatg tctactttag gttttttcgg cataaataaa gttcccttca
     9061 aagttttcat tttttcaatg tctactttaa agggaacatt tcacctctat tattagtgta
     9121 ttagtggtct ttcttgttgt ttagttcttc tttaattttt tcaggagatt ttaaattttc
     9181 agcgttcttt aattttccta agacttcatt acttcctgtc aatcctccag tgaattgata
     9241 gttttcttca ggtgaattgt aagcaataaa atcatagtct tttttactgt taatgtatcc
     9301 atagatttca atatcaccca taggccctgt ttttaaatta gtgaaatgta cagttttgat
     9361 ggtattgggt tctttagtat tatgctttag ataaagcgta atgcgctcct gctgttcttt
     9421 gtagtaaatt tctttttgtt tttctttttc gtcatgtttc atttttaaat agatacctcc
     9481 agtaactata gaaatgcata aaataacacc gataattata aaaatagttt ttttgggcat
     9541 taaaccagct cctaatttta agtgaaagat aaaattttta tacttctatg gttatgaatg
     9601 ttatagatta aagattatag gactaaacta atcttctcca gtaacctaac tattatcatt
     9661 ccctataatt gagcattaaa ttaaaaagac aattaccaaa tactctctta caattaaata
     9721 tagtttaaca ccttgctaac aaaaataaaa ttaaacttat tttccaa
//
LOCUS       DAPIDM010000099         9654 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000099 DAPIDM010000000
VERSION     DAPIDM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.099"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            90..725
                     /locus_tag="RJE42_001957"
     CDS             90..725
                     /locus_tag="RJE42_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDV5877367.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1029..2108
                     /locus_tag="RJE42_001958"
     CDS             1029..2108
                     /locus_tag="RJE42_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDV5877368.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITVQSIGLLILMTTGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDISQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVQQQTQIV"
     gene            2168..2392
                     /locus_tag="RJE42_001959"
     CDS             2168..2392
                     /locus_tag="RJE42_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV5877369.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2601..3849
                     /locus_tag="RJE42_001960"
                     /pseudo
     CDS             2601..3849
                     /locus_tag="RJE42_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            4033..4983
                     /locus_tag="RJE42_001961"
     CDS             4033..4983
                     /locus_tag="RJE42_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV5877370.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5132..6616
                     /locus_tag="RJE42_001962"
     CDS             5132..6616
                     /locus_tag="RJE42_001962"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV5877371.1"
                     /translation="MTEWTREERYQRIEDVDTDYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLFNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFNNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6613..7572
                     /locus_tag="RJE42_001963"
     CDS             6613..7572
                     /locus_tag="RJE42_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDV5877372.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAHMITQLGNDAFGDSIVETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LIQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQVF"
     gene            complement(7946..8662)
                     /locus_tag="RJE42_001964"
     CDS             complement(7946..8662)
                     /locus_tag="RJE42_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5877373.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8681..>9654)
                     /locus_tag="RJE42_001965"
     CDS             complement(8681..>9654)
                     /locus_tag="RJE42_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDV5877374.1"
                     /translation="DSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFL
                     IVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMK
                     YKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEFIREDDMPGLRDYFNKN
                     IVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVTRINLN
                     MIDLSRSIGIILDNAIEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQ
                     ESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3357 a         1327 c         1795 g         3175 t
ORIGIN      
        1 tatttgtttt ttaataatct aacaattacg ctctaactcg tttgaataca tagatataat
       61 tcattcaaac acgaaggagg aaatagcgaa tgagtgacca agttaaaatt cctcgagcaa
      121 ctttaaaacg tttgccgtta tattatagat ttgtcagttc attaaaatct aaaggtatag
      181 atcgtgtaaa ttcaaaagcg attagcgatg cgttacaaat tgactcggca acaattcgtc
      241 gtgacttttc atattttggc gaattaggta aaaaagggta cggatataat atagatagtt
      301 tattggattt ctttaaatct gaactaagcg agagtgacat gatcaaaatc gcaattgtcg
      361 gagttgggaa cctagggaaa gctttgctca catataactt ttcaatacat gacgatatga
      421 cgattacaga agcgtttgac gtaaaagaag atgttattgg ccagaaaata gggaacgtta
      481 ttgtaaaaga taacgatgaa ttaataacaa cattgaagaa ggaagaaata gatgttgtga
      541 ttctaactac accagaaaga gttgcacaga aagttgcaga tgaactcgtc caagctggtg
      601 tgaaaggtat tttaaacttc actcctggta gaataaatac gccttcagat gtgcaagtac
      661 atcaaattga cttaggtata gaattacagt cattattatt ctttatgaaa aattacagtg
      721 aataaatgtg atgttagctt tgaatgatta accttgttat atctttaatt aattatagaa
      781 cagtctggga ctaagtatgt ctcaggcttt ttctgttatg tttgaaaatg aaagggaaaa
      841 ctgttgtgtt gataaatata atataggtaa taaattaaca cgtagagtta taatgatgaa
      901 tcgtaattaa tcgcatttct tatatgaaca taatataaat atcaaaaata ttgtgtataa
      961 ggttgaaaac aagaaaatca tcgcctataa tacatactaa acttatcgga aattaggggg
     1021 ttaggacgat gtggatgatt tttagtggct taattgtcgg gggactctta ggttttgtga
     1081 tgcaaagaac aagattttgt ttaacaggtg gctttcgaga tatgtatgtg caaaagaata
     1141 ataaaatgtt ctatgcatta ttaatcgcta ttactgttca aagtatagga ttattgattt
     1201 tgatgacaac aggtatttta caaattcctg cacatagttt tccaatattg ggaacagtta
     1261 taggttcttt tatttttgga attggaatag tattggctgg agggtgtgca acaggtactt
     1321 ggtatcgcgc tggtgaaggg ctaattggta gttggattgc attagtatta tatgctgtta
     1381 ctgcagcaat cactaaaaca gggattttaa agccagtaat ggataaaatt aatcaaccaa
     1441 cgaatgtaaa tagtgatata tctcaaacaa cgggcattcc gttttgggga ttagtcgtta
     1501 tattaactat aatcaccatt tttctcgttg taagaacgct caataataag aaagttcgag
     1561 ttgctgttcc aaaactaaag caacgttata caggtattag atattacctt ttcgaaaaac
     1621 gataccatcc atttattgca gcaattgcaa ttggacttat cgcactctta gcttggccaa
     1681 tgagtgcatc aactggaaga aatgacggtt taggtataac aacgccatca gcaaatttag
     1741 tacacttttt gattacaggt gaaactaaat ttattgattg gggtgtcttt ttagttctag
     1801 gaattttcat tggttcatat attgcagcta gaggatcaag agaatttaaa tggcgattgc
     1861 cagacaagat tacaatacga aacagtgcta ttggtggcat atgtatggga tttggtgcgt
     1921 cagttgctgg tggttgttct atcggtaacg gtttggttga aacggcaacg atgacttggc
     1981 aaggatggat tgcgctagca tcgatgatag ttggggtatg gacaatgagt cattttatct
     2041 ttgttcgtcc aatgaaaaaa gtacaacaac aatctgcaaa ggtccaacag caaacgcaaa
     2101 tagtatagaa ggttattatg ccaatgatgt tgatcaaata aaagtgattg gaaaaggaga
     2161 aatagctatg atacacgaat taggtacagt aggaatggta tgtccatttc cgttaattga
     2221 agcgcaaaag aaaatggcaa cattgcaatc tggagatgaa ttaaaaattg attttgattg
     2281 cacgcaagcg acggaagcca ttccaaattg ggctgcagaa aatggatatc ctgtaacaaa
     2341 ctatgaacaa attgataatg cttcatggac aattacaatt caaaaagttt aacgatatca
     2401 ttttaacaat aaaatagata ttagagttta ggactacttc cgctacttta aaagcgagta
     2461 agtagtcttt tttgttttag ttcatgaaat cattttttat atagcgtggc atattttgtt
     2521 ccaaaagatg taataaaact ttacgcattt ttgcttttta taaattatca gattattatg
     2581 aaaacaaggg agtggtaagt atgaatctta acgatacgat atttatgttt ttgtgtacat
     2641 tattagtttg gctaatgaca ccaggattaa gtttatttta tggtgggtta gttcaatcta
     2701 aaaatgcgct aaatactgtc atgcaaagta tggcagcaat tgtgcttgtt acatttgtat
     2761 ggataacagt tggttttaca attagttttg gggaagggaa tttatggttt ggtaactggg
     2821 aatatacatt tcttaatcat gtaggtttcg cgacacaaga agatattagt ccgcatattc
     2881 cattggcatt atttatgtta ttccaaatga tgttttgtac gattgcaatc tcaatattat
     2941 caggttcaat cgctgagaaa atgaagttta ttccttattt attatttgta gtaatatgga
     3001 ctgctcttgt atacagtcca gtagcacatt gggtatgggg cggcggttgg ataaacaaac
     3061 tcggtgtatt agatttcgct ggaggtacag tcgttcatat tacatcaggt gtttctggtt
     3121 tagtattagc tataatgatt ggaaaaggaa acaaacattc tgaatcaaca ccacataatc
     3181 ttatcattac gttgataggc ggtatattcg tgtggattgg ttggtatgga tttaatgttg
     3241 gcagtgcttt tacatttgat aaaattgcaa tgcttgcatt tacgaataca gttatttctg
     3301 ccagtgccgg ggctattggt tggttaattt tagaatattt ttaaaaagac gacaagttta
     3361 cttggacttt tactcggtgc attagcagga ttagttgtta ttacacctgc agctggatat
     3421 gtaacatatc ttagtgcaac aatcatggct ttaataggag gtatctgttg ttatattgtc
     3481 atcaattaca taaaggtaaa attaaagtat catgatgcat tagatgcatt tggtatccac
     3541 ggtgttggcg gtattattgg tgcagtatta acagcctttt tccaaagtaa aaaagcaaat
     3601 ccagatattg aaaatggttt tatttatact ggtgacgtgc atattatact tgtgcaaata
     3661 ttatgtgtaa cagcagttgt aatttttagc atcgtcatga cgtttattat tgcaaaagta
     3721 attaaattaa ttacaccatt atctgttaca gaacaagaaa cgaatatagg attagacaag
     3781 attgttcatg gtgaacatgc ttactttgaa ggtgagctaa atagattcaa taaacatatt
     3841 cgatattaga tttatatata catagaatat tcattgtcct gacatttaac taaaggttga
     3901 tgttgggaca ttttgttata cagaaatttt attttgaaat ctttttatga aagaagcaga
     3961 aatattattt aaagcggtta cacatatgct aaaataaggc taagtgtcac aaataatgat
     4021 aggtgaataa gtatgaaaaa tatatctgat attgccaaat tggcaggcgt ttcaaaaagt
     4081 acagtatcta gatttttaaa taatggatct gtcagtaaaa aaacaagtga aaaattaaca
     4141 aaaattatag cagaacatga ctatcaaccg aatcaatttg ctcaaagctt aagagcgaga
     4201 caaacacacc ttattggtgc cattatacct agaatgaatt catatgcagt agatgaaaca
     4261 atcaaaggat tggcaaaaca atgccaaaaa tatgaatcac aattaatttt aaattataca
     4321 ggtttaaata tcgaagcaga aatacaagcg cttgaaacat tagcacgcag taaagtagat
     4381 ggtattgttt taatggctac agacataaca gagagacata ttgaagtcat taataaaatg
     4441 aatgtaccaa tcgttattgt tggtcaacaa catgaacaac tgcatagtat tgtgcatgat
     4501 gattataaag caggtcaaat tataggtgaa tggattggtc aacagggata tcaacaagtt
     4561 gaagtgttta gtgtgagtga aaaagatatt gcagttggta tacatagaaa acgtggttta
     4621 cttgaccagt tagccaaata tcaaattaaa cctaatatac atgaaacaaa ttttacttat
     4681 gtggaagcac aaaaagatgt tgcaaatgtt ttggaaaatg tagagcaagt agatgcggtt
     4741 gttggagcaa ctgatacgat tgcattagct gcctataaat attattctga taaaaaagat
     4801 gttatgaaac cacatcaaat atatggtttt ggtggtgacc caatgacaca attagtgtct
     4861 ccatcgataa aaacaattca ttataattat tttgaagctg gccaatgcgc gatggaagag
     4921 atacaacaga tgcttaaaaa gcaagatatg ccatatagcg tcacagtaga tgttaatatt
     4981 tagacgctgt attttttaaa atgaatgtgg aaccgatacc ataaaaatat aaatggatag
     5041 gttaaaagtt aaagaacgta ggtaaaattt gctataatag aatataaatt gttaacagca
     5101 taaattataa aaggaggact gggtaaatat tatgaccgaa tggactagag aagaacgtta
     5161 tcaacgaatc gaagacgttg atactgatta ttttaaaaca ttaaaacaac aagttgatca
     5221 atcaaaattt cgtcaacaat ttcatataca accagaaaca ggcttattaa acgatcccaa
     5281 cggacttatt ttttataaag gaaagtatta tgtttcacat caatggttcc cattaggcgc
     5341 agtacatggc ttaaagtatt ggtataacta cacgagtgat gacttaataa actttaaagc
     5401 tgaagggcca attttaaatc cagataccaa atatgacagc catggtgttt atagcggtag
     5461 cgcttttgaa tataatgggc atttatatta tatgtacaca ggaaatcatc gagataatca
     5521 ttggcaacgc catgcgagtc agatgatcgc acgattgaaa gaagacggtt cagttgaaaa
     5581 gtttccaaag ccagtaatta gccagcaacc agaaggatat acaagtcatt ttagagatcc
     5641 taaagttttt aaatatggtg agaaatatta tgcgattatt ggcgcacaaa ataatgatca
     5701 gcaaggtcga ttattacttt ttaatactga agatataatt aattggcatt atttaggtga
     5761 aataaataca gagttagatg attttggata tatgtgggaa tgtccagatt actttaatgt
     5821 agataatcaa gatgtcatac ttatttgtcc acaaggtatt gaaccaaaag gcgatcagtt
     5881 caacaatatt tatcaaagtg gttatatact tggaaagttt gatattgaaa agttaacata
     5941 tgaacatgaa aattttgtcg agcttgataa tggctttgat ttctatgcac ctcaaacatt
     6001 tttagatgaa aaaggccgac gagtactaat tggatggatg gggttaccgg aaatcgaata
     6061 tcctactgat aatgaaggat gggcccattg cttaacaatt cctcgtgtat taaatgtaga
     6121 aaatggtcaa cttaagcaac gtccatatcc agcgttggaa aaattacgtc acaataaaga
     6181 gacagcatta ggctacgcaa ataaatttac tcgaaaatta catccgtatg aaggtaaaca
     6241 gtatgaatta atcatagata ttttggataa tgatgctacc gaagtgtact ttgaattacg
     6301 tacgtctaag acttcttcaa cattaatcgc ttataacaag cgtgaaaata aaataacatt
     6361 agatcgcagc gacagtggtt tattgcctac aaatgttgaa ggtacgacgc gtagtacgat
     6421 attagaaacg ccattaaagc aattacaaat ttttgttgat acatctagta tagaaatttt
     6481 ctgtaatgat ggggaacgtg ttttgacatc tcgaattttc ccgacagaag acgcactagg
     6541 tattaaaaca tcaacagaat cagggcaagt atatttacaa tttactaaat atgatttaaa
     6601 ggatgaacat aaatgagacg tttattttca ataggagaag cgttaattga ttttattcca
     6661 aatattacaa atgcgaattt aaaagatgtt caaacattta cgaagcaaat tggtggcgcg
     6721 ccatgcaatg tagcttgtac agttcaaaag ttaggacaac aagcgcatat gattacacaa
     6781 ttaggtaatg atgcatttgg agatagcatt gttgaaacga tttcaagtat tggtgtagat
     6841 gtatcaaaag tgtatcgcac gaatgaagca aacactgcgt tagcttttgt gagtttgact
     6901 gaagcgggag agcgagattt ttctttttat cgtaagccct ctgcagatat gttatttgaa
     6961 ccaagttttg tgaatgatat tgatgtgaat gaaaatgatg tagtacattt ttgttctgtt
     7021 gatttggtcg atagtccgat gagagatgca cattatcaat tgattaccaa aacattaaat
     7081 gcaaatggta ctgtcgtatt tgatcctaat gtaagattgc cattatggga taacgctgaa
     7141 gatttaagac aaactattca tacctttttg ccattggctc atattgtaaa agtttcagat
     7201 gaagaattag aatttattac aggtattcat gatgaaaatg aagccattca atcattattt
     7261 acaggtaatg tcactgtagt catttatacg aaaggagcag atggtgctgc cgtatatttg
     7321 aaaaatggta ttaatcatta tcatagtggc tataaggtaa aacctgttga tacaactggg
     7381 gcaggggatg cctttattgg tgcagttatt agcaggattt tagcaaccga tgtgttaaat
     7441 ttaattcagt tatttgaaaa tgaaggtgag gaaattttag cgtttagcaa tcgcgttgct
     7501 gcaatagtga caacgaagta tggtgctatt aatagtttgc caacattaca agaggttgaa
     7561 caagtatttt aaataattca tttataaaac agtgtaggaa acaagttgaa tcttgtgcta
     7621 cactgttttt ttgcgagcaa aataagattc attgggtagg atattgtagc attttattta
     7681 aggaaactgt cgatttcaaa tttagtatat tttgttaaaa attgcgccat aggattgtag
     7741 agtgaaatag taattgataa attatgtagt aataggaata ggtatacaat gaaacgttgt
     7801 atcgttttat ttaaatttac gctttaaatt aaatttttgc tattaaaaaa taaaaagcca
     7861 gctatacagt gcatttgcta gttatcttgt taaaatccaa caagatttac aattgaatac
     7921 gccgttaact gactttatta tcttattata tttttttaac gtttctcacc gatgcatagc
     7981 agtgttcttt atttttaaaa taaacaatac gctctttcga atcaatagat tcgatattat
     8041 gacgattgac gacaaagcta ttatgacatc tgaagaaacg atcatctaat tgactcagtt
     8101 cttttaaatt accataaaat tcaatttgac ggttatctaa atgggcaatg agtctgtgag
     8161 attttgttga tgattcaaaa aacataatat catcatattg aacatacact gaattactgc
     8221 cacgttttaa ctcaatcgtt tcaacgctat tatcttttga taataattgt aagcgtgtat
     8281 gtgcagtttc taaacaatct ataattcgag ttcttaattc tgctggatca tctttaaaaa
     8341 taaaatccat cgctgcaact ttgtagacaa atgttaaata cgtaagttca ctgtgactcg
     8401 taacgaaaat aatgttacca actgggtcat gcttacgaat ttcactgcct aatttgatac
     8461 cattaatatc agttgaaagt tgaatatcta aaaagtaaca gcctatgtca ttcatatttt
     8521 tagcttgctc aagcacctca taaggattat cagttgcgag ggcaatttcc ataggctttt
     8581 cttctatcat tatataattt ttaataatgg taaccatgtt ttctctttgt tttggatcgt
     8641 cttcgcaaat gaaaattttc atacattcac atccttatgg ctagttgtta ataatttcaa
     8701 ccttttgaat aaagaaacca ttctcgataa ttgtatctaa taagacattg tctgcattat
     8761 cagcaatttc ttttaaagtt gatagaccta aaccacgacc ttcaccttta gtagaaaaac
     8821 tttcttggaa caattcatga atacgtggta tatcatctgc acatttattc ataacaataa
     8881 acgttactga attttcgctt tcaataaatg caactcgaat gataggatca tcaatttcac
     8941 ttgatgcctc tattgcgtta tcaagaataa taccaatact gcgacttaaa tcgatcatat
     9001 tcaaattaat gcgagttact tcatctggta tttcgatact aatcggtata ttcatttctt
     9061 gtgcacgtaa aattttagct gtgattaagc ctttaatctc acgtacttta agattctcaa
     9121 taccatttaa ttttatagca ttcatttgta aattgtcttt cataggtaca atatttttat
     9181 tgaaataatc acgtaggcca ggcatgtcat cttctcgaat gaattctgaa agcgttgtta
     9241 agatattgac ataatcgtga cggaacttgc gcatttcgtt gttgatagct tcaatcttca
     9301 atgtatattc ataataggtt tcaatttctt cttgattacg tttatatttc atctctttaa
     9361 gtagaaattg agaaataaca aatgttaata tacttaaaaa tatagtgata ccaataaaaa
     9421 taaaagaata ctgccttatt actttagctt catccgagtt tatttgtgaa taaaagaaaa
     9481 ataatgaaaa agtaagcagt aagatagtcg aaataactat taaaaatcct ttgtttagta
     9541 ttagatatgg tgtgctaatt tttttgagaa ctctatttat tatatatgag aatagtatac
     9601 taatagtcac ataaactaca aaaaagctag ggaatattac aaatatacta tcag
//
LOCUS       DAPIDM010000100         9587 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000100 DAPIDM010000000
VERSION     DAPIDM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.100"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..4024
                     /locus_tag="RJE42_001966"
     CDS             <1..4024
                     /locus_tag="RJE42_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDV5877375.1"
                     /translation="GTTLIFGIGSQEAKAAEVTNKEMKEDATSVNNDQVSKKVDTGQL
                     NNDGNTSKVDTEQLKDENINKVANQKEVTRVENSVASEKNNNSQPSENGKLQTSDVKK
                     TEANNTTLDDQVTTTVNSQQLNTDNTTSNATMSSAPINVSKDDLKINNEEVRNVGEKS
                     DNADNANGSDVIMPKSTAPKRLNTRMRMAAIQSTSTDSKNVNDLITSNTTLTVVDADN
                     SKTIVPAQDYLSLKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNN
                     GETIATAKHDTANNLITYTFTDYVDRFNSVKMGINYSVYMDADTIPVDKKDVPFSVTI
                     GNQTTTTTADITYPAYKEADNNSIGSAFTETVSHAGNAEDPGYYNQVVYVNPMDKDLK
                     GANLKVEAYHPKYPANIGQINQNVTNIKIYRVPEGYTLNKGYDVNANDLVDVTDEFKN
                     KMTYGTNQSVNIDFGDITSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKTVSTGNA
                     LGFTNNQSGGAGQEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEA
                     VTKEDGSYLIPNLPNGDYRVEFSNLPQGYEVTPSKQGNNEELDSNGVSSVITVNGKDN
                     LSADLGIYKPKYNLGDYVWEDTNKNGIQDQDEKGNVLKTVTTDADGKYKFTDLDNGNY
                     KVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNYV
                     WEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFE
                     TPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNK
                     NGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYT
                     PTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQD
                     STEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTN
                     TTEDDKDADGGEVDVTITDHDDFTLDNGYFEEETSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDAGKHTPVKPMSATKDHQNKAKALPETGSENNGSNNATLFG
                     GLFAVLGSLLLFGRRKKQNK"
     gene            4422..7895
                     /gene="sdrE"
                     /locus_tag="RJE42_001967"
     CDS             4422..7895
                     /gene="sdrE"
                     /locus_tag="RJE42_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HDV5877376.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDEASASDNEEVVSETENNSTTENTSTNPIKKETNTDSQP
                     ETKEESTTSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDSNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVN
                     DLIKVTKQTIKVGDGKDDVVAAHDGEEIEYDSEFTIDNKVKAGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKT
                     VPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQNIEQQIYVN
                     PLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSNQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGNIDSAYIIKVVSKYTPTSDDELDIAQGASMR
                     TTDKNNYYNYAGYSNFIVTSTDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKENGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKIIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTLKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTL
                     QNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEV
                     DVTITDHDDFTIDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDTGKHIPVKPMSATKDHDNKAKALPETGSENNGSNNATLFGGLFAALGSL
                     LLFGRRKKQNK"
     gene            8014..9486
                     /locus_tag="RJE42_001968"
     CDS             8014..9486
                     /locus_tag="RJE42_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV5877377.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDLFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHQLNYVNYFDQQRRKIKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKTTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGTGNDIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDSNQKDKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLLEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSLGTPVISYDVDYGPSELIQDGFNGYLVPPGDINQMVE
                     KVDQLLNNSQMMQQFSINSIESAQQYNATTISIKWQNILN"
BASE COUNT         3841 a         1452 c         1718 g         2576 t
ORIGIN      
        1 aggtacaaca ttaatttttg gtatagggag ccaagaagct aaagctgcag aagtaaccaa
       61 caaggaaatg aaggaagatg caacatcagt aaataatgat caagtatcta aaaaagttga
      121 tacagggcaa ctaaataatg acggtaatac tagtaaagtt gatacagagc aactaaaaga
      181 tgagaatatt aataaagttg ctaatcaaaa agaagtaact agagtagaaa atagtgtcgc
      241 ttcagaaaaa aataataact ctcagccatc tgaaaatgga aaattacaaa cgtcggatgt
      301 taaaaaaact gaagctaata atacaacttt agatgatcaa gttactacta cagttaattc
      361 acagcaatta aatactgata acacaacttc aaatgcaaca atgtcgtcag caccaattaa
      421 tgtaagtaaa gatgacttaa aaattaataa tgaagaagta agaaatgtag gagaaaaaag
      481 cgataatgct gacaatgcaa atggttcaga tgtgattatg ccaaaaagta cagcacctaa
      541 acgtttgaat acaagaatgc gtatggcagc aatacaatca acctcaacag attctaaaaa
      601 tgttaatgat ttaatcacat caaatacaac attaactgtc gttgatgcag ataatagtaa
      661 aactattgta cctgctcaag attatctatc attgaaatct caaattacag ttgatgacaa
      721 agttaaatcg ggtgattatt tcacaattaa atattcagat actgttcaag tatatggttt
      781 gaatccagaa gatattaaaa atattggtga tataaaagat ccaaataatg gggaaacaat
      841 tgcgactgca aaacacgata ctgcaaataa tttaattaca tatacattta cagattatgt
      901 tgatcgattt aattcagtga aaatgggaat taattactca gtttacatgg atgcagatac
      961 aattcctgta gacaagaaag atgttccttt tagtgtaact attggaaatc aaactacaac
     1021 tactacagca gatattactt atccggctta taaagaagct gataataatt caatagggtc
     1081 agcttttaca gaaacagttt ctcatgcagg aaatgctgaa gatcctggtt attataacca
     1141 agtagtttat gtgaatccta tggataaaga tttaaaaggg gctaatttaa aagttgaagc
     1201 gtatcatccg aaatatcccg ctaatattgg tcaaattaat caaaacgtta ctaatataaa
     1261 aatttatcgt gttcctgaag gatatacatt gaataaggga tacgacgtta atgctaatga
     1321 tttagtagac gtaaccgatg aatttaaaaa taaaatgacc tatggaacaa atcaaagtgt
     1381 taatatcgat tttggtgata ttacatcagc gtatgttgta atggttaata caaaattcca
     1441 atatacaaat agtgaaagtc caacacttgt tcaaatggct actttatctt ctacaggtaa
     1501 taaaacagtt tctactggta atgctttagg attcactaat aaccaaagtg gcggcgctgg
     1561 tcaagaagta tataaaattg gtaactacgt atgggaagat acaaataaaa acggtgttca
     1621 agaattagga gaaaaaggcg ttggcaatgt aactgtaact gtatttgata ataatacaaa
     1681 tacaaaagta ggagaagcag ttactaaaga agatgggtca tacttgattc caaacttacc
     1741 taatggagat taccgtgtag aattttcaaa tttaccacaa ggttatgaag taaccccttc
     1801 aaaacaaggt aataacgaag aattagattc aaacggtgta tcatcagtta ttacagttaa
     1861 tggcaaagat aatttatctg ctgacttagg tatttacaaa cctaaataca atctaggcga
     1921 ctatgtatgg gaagatacaa ataaaaatgg tatccaagat caagatgaaa aaggtaacgt
     1981 gttaaaaaca gtaacaacag atgctgacgg taagtataaa tttactgatt tagataacgg
     2041 taattataaa gttgaattta ctacaccaga aggctataca ccgactacag taacatccgg
     2101 tagcgatatt gaaaaagact ctaatggttt aacaacaaca ggtgttatta atggtgctga
     2161 taacatgaca ttagatagtg gattctacaa aacaccaaaa tataatttag gtaattatgt
     2221 atgggaagat acaaataaag atggtaagca ggattcaact gaaaaaggta tttcaggcgt
     2281 aacagttaca ttgaaaaatg aaaacggtga agttttacaa acaactaaaa cagataaaga
     2341 tggtaaatat caatttactg gattagaaaa tggaacatac aaagttgaat tcgaaacacc
     2401 atcaggttac acaccaacac aagtaggttc aggaactgat gaaggcatag attcaaatgg
     2461 gacatcaaca actggtgtca ttaaagataa agataacgat actattgact ctggtttcta
     2521 caaaccgact tacaatttag gtgactatgt atgggaagat acaaataaaa acggtgttca
     2581 agataaagat gaaaagggca tttcaggtgt aacagttacg ttaaaagatg aaaacgacaa
     2641 agttttaaaa acagtaacaa cagatgaaaa tggtaaatat caattcactg atttaaacaa
     2701 tggaacttat aaagttgaat tcgagacacc atcaggttat acaccaactt cagtaacttc
     2761 tggaaatgat actgaaaaag attctaatgg tttaactaca acaggtgtca ttaaagatgc
     2821 agataacatg acattagata gtggattcta caaaacgcca aaatatagtt taggtgatta
     2881 tgtttggtac gacagtaata aagatggtaa acaagattcg actgaaaaag gaattaaagg
     2941 tgttaaagtt actttgcaaa atgaaaaagg cgaagtaatt ggtacaactg aaacagatga
     3001 aaatggtaaa taccgctttg ataatttaga tagtggtaaa tacaaagtta tctttgaaaa
     3061 gcctgctggc ttaactcaaa caggtacaaa tacaactgaa gatgataaag atgcagatgg
     3121 tggcgaagtt gatgtaacaa ttacggatca tgatgatttc acacttgata acggatactt
     3181 cgaagaagag acatcagata gcgactcaga ctcagacagt gattcagatt cggacagcga
     3241 ctcagattca gacagcgact cagactcgga tagtgattca gattcagata gcgactcaga
     3301 ttcagacagc gactcagact cggatagtga ttcagactca gacagtgact cagactcaga
     3361 ttcagatagc gattcagact cagacagcga ttcagactca gatagtgatt cagattcaga
     3421 cagcgactca gattcagata gcgactcaga ttcagacagc gactcagatt cagacagcga
     3481 ctcagactca gacagtgatt cagactcaga tagtgattca gattcagata gcgactcaga
     3541 ttcagacagt gattcagact cagacagcga ttcggactca gacagtgact cagattcaga
     3601 cagtgattcg gactcagata gcgattcaga ctcagatagc gactcagatt cagacagcga
     3661 ctcagactcg gatagcgact cagattcaga tagcgattca gactcagaca gtgattcaga
     3721 ctcagacagc gactcagatt cagatagcga ttcggattca gatagtgact cagattcaga
     3781 cagcgactca gattcagaca gtgattcgga ctcagactca gacagcgatt cagattcaga
     3841 tgcaggaaaa catacacctg tgaaacctat gagtgctact aaagaccatc aaaataaagc
     3901 aaaagcatta ccagaaacag gtagtgaaaa taacggctca aataacgcaa cgctatttgg
     3961 cggattattc gcagtattag gttcattatt gttatttggt cgtcgtaaaa aacagaataa
     4021 ataatttgct taaccaaacc aggtccgtga ggcctggttt ttttatttca aaatttatga
     4081 aatgtatctt atgtttgaga ttaaatagtg caatatttta aaagatataa catacatcaa
     4141 cataaattta attcatcttt acattaaaag tacagacaaa taagaaaagc aattaaacac
     4201 agtttcaaac catttaaaac aattagtata aaaatcaaat agataattaa aaatcaataa
     4261 aattaagtta ttcaaaccaa agtgtaaaat aacagttgaa aataaataaa tttatttaaa
     4321 gtattgtgct ttatctaaaa atgtattaca atgggaatac aaatttttat atgtaaaaat
     4381 attagttttt tgcaaatatt gatattttaa aggagattta tatgattaac agggataaca
     4441 aaaaggcaat aacaaaaaag ggtatgattt cgaatcgctt aaacaaattc tcgattagaa
     4501 agtatactgt aggaactgca tcgattttag taggtacgac attgattttt ggtctaggga
     4561 accaagaagc taaagctgct gaaaacacta gtacagaaaa tgctaagcaa gatgaggcat
     4621 cggctagtga taatgaagaa gtagtgtcag aaactgaaaa taattcgaca acagaaaata
     4681 cttcaacaaa tccaattaag aaagaaacaa atactgattc acaaccagaa actaaagaag
     4741 aatcaactac atcaagtact caaaaacagc aaaataacgt tacagctaca actgaaacta
     4801 agcctcaaaa cattgaaaaa gaaaatgtta aaccttcaac tgataaaact gcgacagaag
     4861 atacatctgt tattttagaa gagaagaaag caccaaataa tacaaataac gatgtaacta
     4921 caaaaccatc tacaagtgaa attcaaacaa aaccaactac acctcaagaa tctacaaata
     4981 ttgaaaattc acaaccgcaa ccaacgcctt caaaagtaga caatcaagtt acagatgcaa
     5041 ctaatccaaa agaaccagta aatgtgtcaa aagaagaact taaaaataat cctgagaaat
     5101 taaaagaatt agttagaaat gatagcaata cagatcgttc aactaaacca gttgctacag
     5161 ctccaacaag tgttgcacca aaacgtttaa acgctaaaat gcgctttgca gttgcacaac
     5221 cagcagcagt tgcttcaaac aatgttaatg atttaatcaa agtgacgaag caaacaatca
     5281 aagttggcga tggtaaggat gatgtagttg ctgcacatga tggcgaagaa attgaatatg
     5341 actcagaatt tacaattgat aataaggtca aagccggcga tacaatgaca attaattatg
     5401 ataagaatgt aattccttcg gatttaacag ataaaaatga tcctatcgat attactgatc
     5461 catcaggaga ggtcattgct aaaggaacat ttgataaagc aactaagcaa atcacatata
     5521 catttacaga ctatgtagat aaatatgaag atataaaatc acgcttaact ctatattcgt
     5581 atattgataa aaaaacagtt ccaaatgaga caagtttgaa tttaacattt gctacagcag
     5641 gtaaagaaac aagccaaaat gtcactgttg attatcaaga tccaatggtt catggtgatt
     5701 caaacattca atctatcttt acaaaattag atgaagataa gcaaaatatt gaacaacaaa
     5761 tttatgttaa tcctttgaaa aaaacagcaa ctaacactaa agttgatata gctggtagtc
     5821 aagtagatga ttatggaaat attaaactag gaaatggtag caccattatt gaccaaaata
     5881 cagaaataaa ggtttataaa gttaactcta atcaacaatt gcctcaaagt aatagaatct
     5941 atgattttag tcaatacgaa gatgtaacaa gtcaatttga taataaaaaa tcatttagta
     6001 ataatgtagc aacattggat tttggtaata ttgattcagc ctatattatt aaagtagtta
     6061 gtaaatatac acctacttct gatgatgagc tagatattgc tcaaggtgct agtatgagaa
     6121 caactgataa aaataattat tataattatg caggatattc aaacttcatc gtaacttcta
     6181 ctgacactgg tggtggtgac ggtactgtta aacctgaaga aaagttatat aaaattggtg
     6241 actatgtatg ggaagacgtt gataaagacg gtgttcaagg tacagattcg aaagaaaagc
     6301 caatggcaaa cgttttagtt acattaactt acccagacgg tacaacaaaa tcagtaagaa
     6361 cagatgctaa cggtcattac gaattcggtg gtttgaaaga cggagaaact tatacagtta
     6421 aattcgaaac gccagctgga tatcttccaa caaaagaaaa tggaacaact gatggtgaaa
     6481 aagactcaaa tggtagttct gtaactgtta aaattaatgg taaagatgat atgtctttag
     6541 acactggttt ttataaagaa cctaaatata atcttggtga ctatgtatgg gaagatacaa
     6601 ataaagatgg tattcaagat gctaatgagc caggtatcaa agatgttaaa gttacgttaa
     6661 aagatagtac tggaaaaata attggtacaa caactacaga tgcatcaggt aaatataaat
     6721 ttactgattt agataatggt aattatactg ttgaattcga aactccagct ggttatacac
     6781 caacacttaa aaatactact gctgaagata aagattctaa tggtttaaca acaacaggtg
     6841 ttattaaaga tgcagataat atgacattag acagtggttt ctataaaaca ccaaaataca
     6901 gtttaggtga ttatgtttgg tacgacagta ataaagacgg caaacaagat tcaactgaaa
     6961 aaggtatcaa agatgtgaca gttacattgc aaaacgaaaa aggcgaagta attggaacaa
     7021 ctaaaacaga tgaaaatggt aaatatcgtt tcgataattt agatagcggt aaatataaag
     7081 ttatttttga aaagcctgct ggcttaacac aaacaggtac aaatacaact gaagatgata
     7141 aagatgcaga tggtggagaa gtagatgtaa caattacaga tcatgatgat tttacaatag
     7201 acaacggtta cttcgaagaa gatacatcag atagcgattc agattcagat agcgactcag
     7261 attcagacag tgactcagat tcagatagcg attcagattc agacagcgat tcggactcag
     7321 acagtgactc agatagcgac tcagactcag atagcgactc agattcagac agtgattcag
     7381 actcagatag cgactcagac tcagatagcg actcagactc agacagtgac tcagattcag
     7441 atagcgactc agactcagac agtgactcag attcagatag cgattcagac tcagatagcg
     7501 actcagactc agacagtgac tcagattcag atagcgattc agattcagac agcgattcgg
     7561 actcagatag cgattcagac tcagatagcg attcagattc agacagcgat tcggactcag
     7621 atagcgactc agattcagat agcgactcag attcagatag tgattcagat tcagacagcg
     7681 actcagactc agatagcgac tcagattcag atacaggaaa acatatacct gttaaaccaa
     7741 tgagtgctac taaagaccat gacaataaag caaaagcatt accagaaaca ggtagtgaaa
     7801 ataacggctc aaataacgca acgttatttg gtggattatt cgcagcatta ggttcattat
     7861 tgttattcgg tcgtcgcaaa aaacaaaaca aataatacaa tatgacccag gtccttgtgg
     7921 cctggttttt ttatgattac acatgcaata gatgtatttt tcatataaaa taaacaataa
     7981 agatacggaa taaaacttat atgaggcgat aatatgaatt acattttagg aacaatttta
     8041 gaaagtaaaa ttacaggtgt agaaaaagcg caaataaata gactgaagtt gttcaaacaa
     8101 cacggcatat cttcaaaatg tgtatatgtt aaatggaatc cttattcata cacatatgcg
     8161 aagcaacatc agattgaaaa tgatctgttt acaatgtatg actattttca aaaagcaatc
     8221 aattataaaa agacaaagca agttaactgg atacagtatt gggaaaagtc atgtaggtac
     8281 acattgaaat ttgtggaaaa ttcaaatgat gtcagaatat atgatgaaga gcaatttata
     8341 atgtatgctc attttttaga taaacagtat catcaattaa actatgtgaa ttattttgat
     8401 caacaaagaa gaaaaattaa acgcgaattg tatgatggaa gaggcttttt aagttgctct
     8461 cgaattttag gtgaaggaca acggattgta ctcgaaaatt actatacgcc taatggggaa
     8521 atcgtcatcc aaaaatattt cgacgatata aaagggaaaa ccacgctcac aaaggttatc
     8581 ttaaatgaag accagcatca acaatttttt gatacagaag atgaattagt tcaatatttt
     8641 ctccatcaat tatgtaaaaa taatgatcaa atcatattag accgtcctca tgaattagga
     8701 aatgttattg cgggattaaa tcaaagtatt ccagttattg ttgtgctcca cagtacacat
     8761 ttatccggca ccggtaatga tataaaaagt ttttataaaa cagtgtttaa taatttaaca
     8821 cgttataaag cgattgttgt atcaacagaa aagcaatgcc aagatatttc acaatatatt
     8881 gaaaataaaa taccagttat caatattccg gttggctacg tggcaaactt aaagtatcaa
     8941 tttgacagca atcaaaagga taaaaatcat atcatatcaa ttgctcgcct cgttgaaaat
     9001 aaacaaatta aacatcaaat tgaagtgatc aagcaattag taacaaaaca tcccaatatt
     9061 caattgaata tttatggaca tggaaatggt ttgttagaat atcgacaact tgtagaagat
     9121 tatcatttat cggaacatgt taaatttcat ggttttaaga cgcatattaa tgaagagatt
     9181 gctaaagcag aactgatgtt atcgacaagt aaaatggaag gttttggatt agcaatttta
     9241 gagtcgcttt cattaggtac accagtgatc agttatgatg tagattatgg gccatcagaa
     9301 ctgattcaag atggatttaa tggctattta gtacctccag gtgacatcaa tcaaatggtt
     9361 gaaaaggttg accaattatt aaataatagc caaatgatgc aacaattttc aattaatagc
     9421 atagaatctg cacaacagta caatgcaact actatcagta taaagtggca aaatatttta
     9481 aactaagtca aaagaaaaaa gcattttcct aaaatcatct atttaacaat ccacgttcat
     9541 aagaactgac aagtttgtta aatgacatag gaaaatgctt tttttat
//
LOCUS       DAPIDM010000101         9527 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000101 DAPIDM010000000
VERSION     DAPIDM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.101"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            153..1322
                     /locus_tag="RJE42_001969"
     CDS             153..1322
                     /locus_tag="RJE42_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDV5877378.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            complement(1430..1858)
                     /locus_tag="RJE42_001970"
     CDS             complement(1430..1858)
                     /locus_tag="RJE42_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HDV5877379.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFTTPSFVTNIYE
                     TDELYYLEAELAGANKEDISIDFNNNVLTIQATRSAKYKSGKLILDERNFESLIRQFD
                     FEAVDKQNITASFENGLLTITLPKIKPSNDTTSSTSIPIS"
     gene            complement(2075..2521)
                     /locus_tag="RJE42_001971"
     CDS             complement(2075..2521)
                     /locus_tag="RJE42_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5877380.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKDYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIVNNLRNFVSKNFDNSDKK"
     gene            2917..3282
                     /locus_tag="RJE42_001972"
     CDS             2917..3282
                     /locus_tag="RJE42_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDV5877381.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKSLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            3464..4741
                     /locus_tag="RJE42_001973"
     CDS             3464..4741
                     /locus_tag="RJE42_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDV5877382.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKDITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(4939..5295)
                     /locus_tag="RJE42_001974"
     CDS             complement(4939..5295)
                     /locus_tag="RJE42_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDV5877383.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(5573..5995)
                     /locus_tag="RJE42_001975"
     CDS             complement(5573..5995)
                     /locus_tag="RJE42_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDV5877384.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(6066..8171)
                     /gene="rsp"
                     /locus_tag="RJE42_001976"
     CDS             complement(6066..8171)
                     /gene="rsp"
                     /locus_tag="RJE42_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDV5877385.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHERGSIQHLLPTSDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         3309 a         1351 c         1472 g         3395 t
ORIGIN      
        1 attttcgttc agtcaactac tgccaatata acttcgtaga gcatggaaca ttgatttatt
       61 tcccaggctc tttaaataac attgacctct aacatatagg cagagtattg atatctatta
      121 aaaaataaat gattttggtg aaggtgaaac gtatgtacaa aacaaaaggt ggctttcaac
      181 ttacattaca aacattaagt ttagtggttg gatttatggc ttggagtatt attgctcctt
      241 taatgccttt tattaaacag gatgtcaatg taactgaagg tcaaatatca atcattttag
      301 cgataccagt tattttggga tcagtgctcc gtgtgccatt tggttattta acaaatattg
      361 ttggtgctaa atgggtattc tttacaagtt ttatcgtatt gttattcccg atatttttct
      421 tgagccaagc tcaaacgccg gggatgttaa tggcatcagg atttttcctt ggcgtaggtg
      481 gcgcaatttt ctctgttggt gttacatcag ttcctaaata tttccctaaa gaaaaagtag
      541 gtctagcaaa tggtatttat ggtatgggga atatcggtac agcagtttct tcatttttag
      601 caccaccgat agcgggtatt attggttggc aaacaacagt tagaagttac ttaattatta
      661 tcgctttatt tgcattaatt atgttcattt ttggtgacac acaagaacgt aaaattaaag
      721 tgccattaat ggcccaaatg aaaacattat ctaaaaacta caaattatat tacttaagtt
      781 attggtattt cattactttt ggtgcttttg ttgcatttgg tattttctta cctaactact
      841 tagtaaatca ttttggaatt gacaaagtag atgctggtat tcgttcaggt gtattcattg
      901 cgctggcaac attcttgaga ccaataggtg gtatactagg tgataaattt aatgcagtta
      961 aagtactgat gattgatttt gttattatga ttatcggtgc cgttatttta ggtatttcag
     1021 accatatcgc attatttact gtaggttgtt taacaataag tatttgtgca ggtattggta
     1081 acggcttaat ttttaaatta gtaccatcat acttctcaaa tgaagcagga tcagcaaatg
     1141 gtatcgtatc aatgatgggt ggtttaggag gattcttccc accgttggtc atcacatacg
     1201 tagctaattt aacaggttca agccatttag catttatttt cttagcggta ttcggatgca
     1261 ttgcattatt tacaatgcgc catttatatc aaaaagaata tggttcattg aaacacagtt
     1321 gatatgtaat acgaagcatt tttaaattta aatgataaag ccttaatacc atgtgtaacg
     1381 ttagtagcat gacattaagg ctttagtaat gtattcattt taaatttaat taagaaattg
     1441 gaatagatgt tgatgaagta gtgtcattgc ttggtttgat tttaggcagg gtaatggtta
     1501 atagcccatt ttcaaaacta gcagtaatat tttgcttatc aacagcttca aaatcaaatt
     1561 ggcgtattaa tgattcgaag tttcgctcat ctaaaatgag ttttccggat ttatatttag
     1621 cacttctagt agcttgaata gtaagcacat tattattgaa atcgatacta atgtcttctt
     1681 tatttgcacc tgctagctca gcttctaagt aatataattc gtctgtttca tatatattcg
     1741 ttacaaatga aggtgttgta aagtaattaa atacttgttt acctaaatct ttaaatgtat
     1801 cacttggatt accgttaaaa aagttttgat tctcgaattg attgaagttc aatatcatca
     1861 cttccattat ataaatttgt attacaaagt cattatatga gtagaataaa aaaagataaa
     1921 gtaaaagggg ggaataaaaa agttataaat ggatagattt gtgcctgata aggaatcagg
     1981 gcaaaagaac tgagcatcga gatttataca agcaataaag ataatgaata aacatagcga
     2041 tgcatacttc tgcccatcat cttatgatag ggagttactt tttgtcggaa ttgtcgaaat
     2101 ttttcgaaac aaaattccgc aaattattaa caatgtctga tgcttctcgt tctgaaatgt
     2161 tgaattcatt aaatacttta acagaaattt cagcaagagg actttttatt gctttacctt
     2221 gttctgttag agaaatttgt aggtttcttt catctttctc ttcacgtgtt cgaacaacgt
     2281 aatctttctt ttctaatttc tttagtaatg gtgtcagtgt tccagaatct aagaatacac
     2341 gttcacctaa ttttttgatg ttaagttttt catcattttc aatcgccatt aaaacaatgt
     2401 aaccagtata ggttaaatca taatccttaa gatagtttgt gtatttttta attatttctt
     2461 ttgaagaaac ataaaataaa aaacacaact gtttgctaag atagctgttt tctacataca
     2521 tccaatcact ccttgttaaa ataaacaata ttatgaagtg taatagttgt caagtactga
     2581 tttcatcgca attatcgaat tgtctataat tgaatttaaa tgttaaataa tgcaaaacat
     2641 atcaatatct tagtattttc atcggtaatg actgatatga ctgatttttt gaattgaaaa
     2701 aaatgtaatt gtatcattct taattgttaa aagtagtcaa ttattttgat agatagtaaa
     2761 atgaaatttg ttattaaatt ttttaaaagc atcgtgtatg taattcgatt tctataataa
     2821 tattgagaag aaaaaattgt tacaatctgg taaaaactct ttattaaata aattgattcg
     2881 ttcatactga atagataagc aaaaaggagt tacataatga agaagaaaaa aaacgcgatt
     2941 aaagttatca ttatattatt tatttcatta attgttgctg tagcattttt ctttggtctg
     3001 aaaacatatc aaggccataa gaatattcaa cttattgaca gttacttgga agagaaaagt
     3061 ctaaaagata aaattaaatc agaaaaaaca gagtatagtg ctaaaaaagg tctcttctat
     3121 aaggaagtta cgtttaaaga tgaacctggt gtaacttatg ttgtgcaacc aatcagtact
     3181 aataaaggat tatttgttga aggctttgat acagagacta agaaaagttt gaaaactgca
     3241 aagcataaat atttcaatca aaattataaa ccatcaaaat agtgaaacgt ataactgatg
     3301 aaacagttta gtgcctgcaa ttttaattat ttaaggtaaa cagtttttgt atggaaatta
     3361 gtgatttttg acatctataa aagataaata ttttaatttt ttatagataa ttatcaattt
     3421 tctgatataa ttgagtgatg tcagatgaag gggagtttgt actatggctc tattcaagag
     3481 aaaaattagc ctacctatgc aagtagttat tgcattagtg ttaggtgttg tcgtaggact
     3541 tttattatat ggacaagaaa acgtagcaaa ttacattaaa ccatttggtg acgtattttt
     3601 aaatttaatt aaaatgatcg ttataccagt tgtattttgc tcactagcgc tttctatttc
     3661 gaacgttggg gaatcgaaaa ctgtagggcg ttatggctgg aaaacaattt tatactttga
     3721 aattattaca acaatcgcaa taggtttagg gattatcttc ggtaacctat ttaaaccagg
     3781 tgctggatta gacccaacaa aattacctaa aggtgatatt tctaaatatc aatcaactgc
     3841 acatgcagca gaacaatcta catatggaaa tcattttatt gataccattg tacatattat
     3901 tccgacaaac ttttttgaag ctttaaataa gggtgaatta ttacctatta tcttcttcgc
     3961 agtattcttt ggattaggat tagctgcagt aggtaaaaaa gcagaaccag ttaaagaatt
     4021 tttaagcgga tcgcttgaag ctgtgttctg gatgattaat aaaattttaa aattagcacc
     4081 acttggagtg tttgcattca tttgtactac aattattaca tttggtgcat cagcattatt
     4141 accactatta aaattagtat tagttgttgt ctttgcaatg gtgttctttg tattcgctat
     4201 actaggacta gttgcatgga tgtgtggtat taatatcatg aatattatta gaatcttgaa
     4261 aagtgaattg cttttagcat tttctacatc aagttcggaa gctgttcttc ctgtaatgat
     4321 gaagaaaatg gaaaactttg gttcaccaaa agatattaca tcctttgtta taccgattgg
     4381 ctatacgttt aacttagacg gatcagcact ttatcaatct attgcagcat tattcgttgc
     4441 acagatgtat ggaatgcact taacattatc agagcaaatt gtgttgatgt taacattaat
     4501 gattacatct aaaggtatgg cggcagtacc aggaacttca atcgttgttt tattaacaac
     4561 attaggcgcc atgggcttac cggcacaagg tttagcatta attattggtg ttgaccgtat
     4621 cttagatatg gttcgtacat gtgtaaacgt tattggtaac gcattatcaa caattgttat
     4681 tgctaaatgg gaaaacgtat acgataaagc aaaaggtcaa gaatatttaa aatcaattta
     4741 aaaaatacta tctgacattt aaagcccctt acaaccttcg gttgttaggg gcttttatgc
     4801 atgcgctaaa gcgcagggca tttgcaaata aactggtata tttattctca aaaaaagcag
     4861 cagtaagata attttcaatt agaaaacatc ttactgctgc ttttaatatc ttatatttta
     4921 ctcataatta aagcttaatt agtgtttcat gtgcattttg cccatgtcat gaattttcat
     4981 cattaaacca tgtggtacat cgtcatgatc aacttttttt actttaacaa ttttatcaga
     5041 atctgctaat ttataaacaa ttacaaagtc gcctttttta actgaaacgt ctgcatctga
     5101 aggtaattta acgttcttct caatgctttc atctttttca ctaatcattt tttcgattgt
     5161 agcattgtct ttcataattc cataatatgt atctttcttt ccaccagtca tcatcatgat
     5221 tactagtaca atacatacaa tcaacatgat ggcaattaaa ctaataccaa gaccaaaacc
     5281 ttttttcttt ttcacggttc atcaatctcc ttaaatgtag ttaattctgt tatacctaaa
     5341 agtacaacag tttgcaaatg cagtatttat attacataaa attaatttaa aaagacacac
     5401 cttaatcaca ataaatgtga ataaaatgtg tctgaaatac tgttatataa taaaaatata
     5461 gttaaaagta ttaaacgctt tcaaaatcga aatatatata aataggatac tgttgaaatt
     5521 tatagctaac atgttcaatt gctggctaat tttaaaacta aaaacttaca tattataaat
     5581 caattttaat aggtgtatct aatgatttat cattcattag cttaatggat tgtggaacta
     5641 ctcttaaaac acataactct gggtcttctt ttgaactgaa aaagcttttg tcttgtgttt
     5701 cccatagcca atcaatcact tcttgttctg taacgatttc gattgtcgct tccatttcaa
     5761 cgaaactacg attagttgtg tcgttatagc ctaataaaac atatgctgct ggattatctt
     5821 taatttcttt gaccttagca gaatggatat tcgttttagt atataaagta agaccatcat
     5881 tataaaagac catatatcta ctattaggtt tgttattata tgcagttgat aatacaccaa
     5941 cttttgacgt tgctagcacg ttttcaattg cttgaattgc ttgtgagtta ctcatcctat
     6001 atcatctcct tatgatataa atagccttta acgtaagaaa ctaaacacaa gaggactgtt
     6061 agtatttagc ttggtttaaa gcaaatatat ttaacctcgt cgtgatttaa cttgagttca
     6121 aatgtacgac cagtttcttc ttgaactgca agctccgttt tagggtaatt cgttttgtct
     6181 aattcatcta gaatttcttt ttctatatag acaagttgat cgcttgttgg aagcaaatgt
     6241 tgaattgaac cacgttcatg attcaaagtt ttaataatca tcaaagaatc ttgaggtaat
     6301 tcattatgga aaatgaagtc ttgttttacg tctgacatat atcggtcatt aattttattg
     6361 aataataaga aatggtagtt attatcttta cgactcaata cataattatt tgtaatttca
     6421 atgtcttcat taacaaaagg tctaaacatt ttatataaat gcattaatgg tagggggcta
     6481 ccatatttgt tgtacaagga aatataattt gaatggtcat ctaataattg atagctaatg
     6541 ccaccgccat atcgcaataa tgcgattaag tgatacacta tgtcagataa ttgaattgga
     6601 ttcttaccat cattagtata tttaaatccg cttacagaca ggttattata tacaaattgt
     6661 gttgatgata tacctgaatc taaaataaat ttcttatact gttcaaaatg agtgtcagtt
     6721 tgatgatagt gttggttttt agtgataaga tgtgttgcag tttttgaatt ttcaatatca
     6781 ataaagtaat aatggaattt aagacgctgc atgacatcat aaacttgttc cacggttgaa
     6841 taagtttcta aaaagccatc gactgtcaca ctatatttaa tttctttatt tttgttttta
     6901 attttaagat gacataaatg aatttcttct gctgtcatag agttagagca aaatacgagc
     6961 ataaatttaa ctaatttctt gcttgtctta tagtcttgat tgccttgtaa aaatgttagt
     7021 attaattgct tcatcgtttc gaattgttgt gttgatttaa tagcgattgc taaacctata
     7081 tttttttcga aaagcttttc aaaacatcta tttaacaggt tgtaattcgt ttggctaatc
     7141 tcaatatcgt gaatatcgtc aatgaaaacg acaggttttg gtaggtgctc aaagttaata
     7201 tcgtaatatt cattgaaaat aaattgaaat agttcattga aattactaaa tcgaacaaat
     7261 actttggcac gctgacttcg atctttcgga ttaaaatcat ctatatgaat atctgttgta
     7321 tttgtagcca gctgagctgt taaatcaatt gttgaaatta actcagtgaa ttgtgatacg
     7381 ttatcttgtt ggaagtgtat ttgtggtggt tcagtaatct tggaaacgag tgaacggaac
     7441 tgtttaggac taaaatgcaa gtaattctta aattgatttg caaaatttgt atgactgtta
     7501 aaaccggcca attcagaaac agttgtaata gaatgttttg tagaaagtaa taaattgatg
     7561 gcgttaacaa gtttaatact tgtaaaataa tctttaaaat tcatacttaa atatcgaaca
     7621 aaaagattag aacaatatga ttcagagatg ttgcaatgca tcgctatatc ttttaaagac
     7681 aagtgcgcat caatattgtc atgaatatag tttacacagt ctataaacac cggattgctt
     7741 aaagcaatat ttggtaaata ttcatgatct attcttataa aagcttcttt aagtaaagtg
     7801 tctataatac tttgacctat attttttccc attaaagaag aacttgttgg tgtactgatt
     7861 aaatcggcaa ttaatgactt aatataatta cttgattgta ataaatgacg atctaaataa
     7921 ccattaaaga aatcatcatc ttgttgataa aaataaaaaa cgggtatcat caattcgatt
     7981 atattttcgc cgtggttaat attataaata tcaccatggt taattaagat aatataatta
     8041 cttacgtctt tggtttcacc attaatcgta atattgagtt tacttgtaag tgaaaatagc
     8101 accataatat tattgatatt acgacttggt attgtcgata atgtatgatg tattttaagt
     8161 tggcatgtca tatttctctc tcctgcctaa tatatattga aatctatcat ttaaatgtaa
     8221 aacatggaaa tttttaaaat catggatagt atacctatta tagttgaata tttttgaaaa
     8281 ggtaatagtg aaaaataaaa taaattaaag aaaactccta ggaaaacgct tgtttttatg
     8341 cattgtgcac aatataaaag taaaattaga aagatattaa gcaaaatttg gaaattttcg
     8401 atagagtcta attaaattat ttggtgcata attaatatag atttcaaacc tatgtatttc
     8461 aaaaaacaat acatagccat tttgaactaa cttgctaatc ttttattgat attcaactat
     8521 gttataaaaa aagcaaattg accaaaatgg aagtttgaat caaaaaacat taaatgtacc
     8581 catgacttaa attttatcgc cattatttaa gtttggacat acacttttgt catcaaacgc
     8641 tgaaaattag ttcgattgaa atcacttctt gttataaaaa ttcttagtag ccctgttaca
     8701 aaatattcgc tacaaattat tcaaatcaac aacaataacc atcaactaag aaattttaaa
     8761 caatctacta aaaaataaag cgtttaaaag ttctgtttag aaaacatcaa gataagtata
     8821 aacatctaga aaagtgaaca tctaagaaaa tccatctagt tattgccgta acaattagaa
     8881 taatcatttg aaaaagtgca ttagacaacg aaattcacca tcaagataat gaagcaacaa
     8941 gaaacagcac acatcaatga attaacacct tagattcatc atgagaaaat gcatggcaca
     9001 actagattta ccatcaagat aattcttaag cagcgaagaa ttaacatcta gaaatgcata
     9061 aacatcttag attttacatc aagagatgcg ataactataa acaactagaa acgtcaccaa
     9121 gatagtgcaa caacaaagaa tcagcatctt gaaatcgcac taacatctaa gaatcaacat
     9181 caagtattat attaacatca tagataactt catcaagaca ggcatccaca tctaagaaac
     9241 tcatctagaa tagctaacac ctaacatcaa agaatattca tcaagaacag tatctaacag
     9301 aactttttga ctatatatga attaattatg ttactagcta aatcatctat tattaacttt
     9361 cacactcaat ttctacatct aatatcaatt tattaacaat agaattagca gcaacaagat
     9421 attacttaaa attactcccc cgaatttaaa atataaacaa tgtaataatt aagggtataa
     9481 attgatttat gctgtaacta attaaatgtg tatggtatct tctaaca
//
LOCUS       DAPIDM010000102         9472 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000102 DAPIDM010000000
VERSION     DAPIDM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.102"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1917)
                     /gene="ltaS"
                     /locus_tag="RJE42_001977"
     CDS             complement(<1..1917)
                     /gene="ltaS"
                     /locus_tag="RJE42_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDV5877386.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETG"
     gene            complement(2391..3395)
                     /locus_tag="RJE42_001978"
     CDS             complement(2391..3395)
                     /locus_tag="RJE42_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDV5877387.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEQMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(3379..4089)
                     /locus_tag="RJE42_001979"
     CDS             complement(3379..4089)
                     /locus_tag="RJE42_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDV5877388.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(4176..4382)
                     /locus_tag="RJE42_001980"
     CDS             complement(4176..4382)
                     /locus_tag="RJE42_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877389.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     IIVYALIRELMPFVVRAMDLMYHQ"
     gene            complement(4448..5056)
                     /locus_tag="RJE42_001981"
     CDS             complement(4448..5056)
                     /locus_tag="RJE42_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDV5877390.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLHAESEFETIVSPLPEKSSFTAKFQVLPKAVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNVVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(5056..6207)
                     /locus_tag="RJE42_001982"
     CDS             complement(5056..6207)
                     /locus_tag="RJE42_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDV5877391.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCSHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(6191..6784)
                     /locus_tag="RJE42_001983"
     CDS             complement(6191..6784)
                     /locus_tag="RJE42_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDV5877392.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAVEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            7135..7803
                     /gene="queC"
                     /locus_tag="RJE42_001984"
     CDS             7135..7803
                     /gene="queC"
                     /locus_tag="RJE42_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV5877393.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            7805..8224
                     /gene="queD"
                     /locus_tag="RJE42_001985"
     CDS             7805..8224
                     /gene="queD"
                     /locus_tag="RJE42_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV5877394.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            8228..8941
                     /gene="queE"
                     /locus_tag="RJE42_001986"
     CDS             8228..8941
                     /gene="queE"
                     /locus_tag="RJE42_001986"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV5877395.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVIAQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDIN
                     HVYVLPQLHTLLWSNKKGV"
     gene            9040..>9472
                     /locus_tag="RJE42_001987"
     CDS             9040..>9472
                     /locus_tag="RJE42_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877396.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDESAENN"
BASE COUNT         3108 a         1590 c         1392 g         3382 t
ORIGIN      
        1 tcctgtttca tatttatact tcgaaggatt tacctttttg aagtctggat ttttgtagaa
       61 tctaaataaa tcaccattaa gcacgttgtc actcatttcg agatcctttt caacttgctt
      121 tttattcttt tcgaaatcag ctggttgagt agttatgagt tcattatttt tattagaata
      181 aatcttacca ttaacatatt tataatcttt tgttataaag tcaccatttc tgaatggaac
      241 tacttgatta tgacctttag agaataaatc agtaccgaac attaaatagt tcttcgtatc
      301 tataccagcc aaatgtaaaa ttgttggcat tacatcgact tgaccagcat attcattatt
      361 gataccacca gatttaccag ggattttaat ccagaaacca gttctgttta aatctgtaaa
      421 tttagctggt gtgatttttt cacctaatag tttttccatg gcattgttat ggttttcaga
      481 gataccatag tggtcaccat aaatcataat cactgaattg tcatataatc cttttttctt
      541 caagtcatta atatattctt ctaatgcttc gtctaaataa cgtgctgttt gaatataacc
      601 atcaactgtt gcatcacctg tgtttgactt ctcaatagtt gcatcctttt catctaatgt
      661 gaatggatag tggttagtca atgtaattaa atgagaatag aatggtgatt tcatcttagc
      721 ttgataatta gcagaatctt taaagaaaat tttgtctttc aagcctaagt ttacaacgtt
      781 tttatctgac atgtcatagt atgttgcatc atagaattta tcgataccaa agtgtttata
      841 tacttggtct ctgttccaga atgttttata gtcaccgtgc atgacatcag atttgtaacc
      901 ttgcttttga tctaaaattg ctggtaatga ctgatacgta ttatctcctt ttaatgaaaa
      961 ggcagaacct tgcggtagac cgtataaact gttatccatt gtaaattcag agtcagatgt
     1021 tttaccttga cctgtttgat ggaaaaagtt agggaagtat gtgaattgct ctttcccact
     1081 tgataatttg tttaaaaacg gtgttacttc ttttccatta acctttttat taattaagaa
     1141 ggtttggaaa ctttctaagt gaatcttaat aatatttttc ttctttgcta ccccataata
     1201 ttctgggtta ggctctgttt gacgttgttt cgtataattt aatacttttg ttaagtcatc
     1261 ttcagatgct aatgcttttt gttgattatt ttcgatagtt ttaacaccat cgtatactgt
     1321 aaagttatac ggtcctaaat atttcactaa atatttatgg tcaaatgtac gtgttaataa
     1381 ttctggtctg tcagtttcag caaaagctaa gtttaagaag aataatgcta ctgaagccgc
     1441 cattacgaca ggaacaaatt tcttactaaa tgcttttgtg tctaaccatt ttgttttaaa
     1501 tattaaaatg aataagtaaa ctaacgtatc aatgaaataa acaaagtcat accatttgaa
     1561 tgatgcacta accgcaccac ccatagattc tacgttacct acttggttta aagtactaaa
     1621 cgttaaaaaa tccgagaaga atctaaagta cacaacattg gcatataata ggaacgtcaa
     1681 taagaagccg cctatgaaca tgaaccaaaa tgcttttttg cctttaaaga ataggaacac
     1741 acttaaaact agtgctacta aactataagg attcatcaat aatattaagt tttgtactaa
     1801 acctttaaca cctaaagaaa aatcaacata ataagaaaaa tacgtcttca aggtaatcgt
     1861 tattacggtt aataagaaga acgcaaaaag actaattttc tttttttgtg aactcatgat
     1921 tctttccccc gttatttaga taataaatct tgcgaaagta cttgaataaa ttagatatta
     1981 acattatgta gttaattaat aagtgcgttt aaaagttaac attgtcataa ttatagtaat
     2041 tactttcaga ttcaagttta taattttcat tatttgtagc tttcaacttt gatttaacaa
     2101 gtattttggg ctacaactca taattgcttg tgtaagtttc tttatttaaa attatcattc
     2161 aattaacaaa tttaaaattt gtaatcaaat tgtaataata agcatagctc aattaataaa
     2221 tatagagtaa ttttagtcaa aattcaagta aaaacacact ttatcttttc attttactgt
     2281 gaaacgaatt agtatatgat acatttcgca gataaaacac agtattattt cattatacat
     2341 taggaggaaa ttatttgcat cggactcgag tatgagctac gtaccaaata ttatttcatc
     2401 atttctaatt gcattctata ttctattcca tggaataatt ggtttaacca gttactatac
     2461 ttaacaaggt tcttttcagc cgttaaaata tctataaatt gaaatttcaa acgtgatcct
     2521 tgcggttttt gtgctaattt cgttaaatga taacttgcga ttgtaccgat ttgcgggtag
     2581 ctacctagcg tgtaatgatc atttaataag ataataggtg tgccatcacg cttaatttgt
     2641 atcgttcctt ttttgacagt ctgatacggt ggcatatctt cataaaaagc tttgattttt
     2701 tcaccttcaa gcatcatccc catgcgattt gctttgctcg ttactttata atcatgtttc
     2761 acaaatcttt gaatggcatc ttctttaaaa tcttccgtac ctttattttt aacgacatga
     2821 aatacatcag acatataatt aaatgacaag gcgtacccat caatgcccca atctgtttgt
     2881 ttcgtgtgag caaggttttc aaataactta tgatgacgag ctgtataatc tctcttaagc
     2941 tttatttcat cgccatcttg taatgttctt cctttaaaac caccaatttt tacattaaag
     3001 tcggttgaat tagatcctaa ccatgcatct aattcaaatc cacctcccac agctaaatac
     3061 actcgcgatg tataacttgt ttctctaaac tttaaaacat cgcctttatc taacaaatac
     3121 aatttatatg gagatatagt catttgctca gtgtaagctt tgacattacc ccctgctaat
     3181 gcaatcagcg taggttctgt aaaacgaatc gttgccattt tattagtcat ttccaaagtt
     3241 gcttcattct tgtcatttgc aactaatcga ttagcaattt catgtgctaa agtatcaagt
     3301 gcaccacatg gaattacacc atcatgttca tatccccttc taccaaagtc ctgaaagcta
     3361 ctgaataagc cacttttttc aattatgatt gacatcttta aacgctccta cttctaattc
     3421 attttcattg ataggtctaa atttaatatt atcgcctaac tttaagcgtg cgaaatctga
     3481 ttctttcgga ttaaaaagtg ataatggtgt ataaccaata actaaccaat cattaattgt
     3541 atctgtagtt acaataccgc attttttccc ttcaagtact acagaaccag ccggaatgaa
     3601 ttttttctgt ttactcgtgt gattaatata caatttctta tttaatccag ttaaataagg
     3661 aaacccaggt gaatatccca tcatcgaaac aaaatattgc gccttagaat gtaattcaat
     3721 aaatgtttct agcttgattt tgtaatgttt taaaagtgat tctaaatccg gaccatattt
     3781 cgaaccatac acaattggta tttcaatcaa atcttgctgg ttaaaatcat gttttatgtt
     3841 taaatgaatc gattctatta gtgctttcat gtataaaaaa ggtgattgta tattatggtg
     3901 tttaatcata tcccttgcgt catagacaat catcatgtct gattctgatg gcacaatttc
     3961 tataataaat ggataatttt gttcaattaa atatgagcga agtgttaata aatcttcggt
     4021 taaactttta gatacttctt tttcaattgc gactacaatg gcttggtcac cttgactata
     4081 tattttcatt tgtacacctc ttcaatattt ggttacttta ctttgatttt taattataga
     4141 atcgattgaa tagttcattt acctactcac taaatttatt ggtgatacat taaatccatt
     4201 gctcgcacaa caaaaggcat gagttcgcga attaatgcgt atacaatgat aaagcttata
     4261 acagtagtaa ttgttgctat caaacgaaca acatatattc tattttcaga tagcaatcga
     4321 ttcactaatc gataatttac gtatacaaga attaacaaca gcacaatata aacaataatc
     4381 atatttggcc ccatttaatt tttaaatttg tttttacttc attttctaca ttattatcat
     4441 actaatttta aaggcaaagg tggacatcgg caacctctcg taaactattt atcaaaaata
     4501 aacgtatctc attgttatga tatttataaa tcaattcgtt tttattaaag tctttttcaa
     4561 caagtttgtc actatctatt aaataatcac gcatgcatcc ttttaagaaa tcatctttat
     4621 aacttggtgt gtaccatttt ccatcctctt caatgacaac gttgccaata tcaaattcaa
     4681 ggaccttgcc gtcctctgaa gttagcaata ttaaatccgt ttcatgattg tgtgctaaat
     4741 gctttcgttc tgtcgtttta ttatttataa aagttggatt aactgctttg ggcaacactt
     4801 gaaattttgc tgtaaaacta cttttctcag gtaaaggtga cactatcgtt tcgaattcgc
     4861 tttcagcatg taatacgatt ttaagtctat attggccact gtgatacttt gttgttacat
     4921 cgtttaattc atttcgccat gcatgttcat caaatttaaa gtttaatcgc tcagaagaac
     4981 attttatgcg attagtatga taagtaagtc tagggatatg tccattatca attttcattg
     5041 tttcaaataa ttgcattata acatctccaa aatcttggtt tttgcgtaaa attcattcac
     5101 ttcatctttt ggcttagaat caattgtaat acctgctccg actccataaa tcgcttgtcc
     5161 atatttatac tcaatagtac gaatcggaat attaaaaatc attttatcat cttcagttgg
     5221 aagtaatagt ccaattgctc cgcagtatat accacgaggt gaactttcta attgtttaat
     5281 atatttcatt gtatttagtt tcggtgcacc tgtaatcgaa ccacaaggaa ataacgatgt
     5341 taaaatttga gttaagtctg tattagtttt taaagttcca cttaccatcg aagtcatttg
     5401 aaatacagtt ttatatgcct caatgaaaaa tagtttatac accttaattg agccactctg
     5461 tgatattctc cctatatcgt ttcttagtaa atcaacaatc atgacatttt ctgcacgatc
     5521 tttcgaagaa gtttgcaatg ttttataata ctgttgatct tcagcttccg ttttacctct
     5581 aggcattgtc cctttcatcg gtttgcttat gataacgtta tcgacattgt taaactgtcc
     5641 tttttgaaaa aataattctg gtgagataga tgctacttgg atttcatctg tttgtaataa
     5701 cgcagtataa ttaccattac taaattgagt taatcgttca tataaagtac taataggata
     5761 ataaatgtta tccgttaagc gcgccgtata gttcacttga tacgtttcgc cttcaacaat
     5821 tgcttgttgg acacgtttaa tattagtcat cataacctca gaagattcaa caaatgaaaa
     5881 atgatgcttt gatacataag aagtttgatg ttcatatgtt gaatttatgc tttccgcttt
     5941 ttcaaaacta taagctactg cataaatatc atctttagct aatgaatgtg aacacataac
     6001 atgattaaag tactttgccg cttcgtaact taaatataac gaaacatatc taccttgtcg
     6061 ttgctgcgct tgtgcaaagt gtatcacttc tcccacatca gccaacttag tggcaacata
     6121 cttctttata aatcccttta attgaatatg gtattgctta tattcatttt cagttaaata
     6181 gtagcgataa ttatattcta ttctcacagt aatcacctac cttcgataaa aataattcaa
     6241 cttgtcgata accgtattca ctcaaaatag attcaggatg atattgcaca ccaaaaaccg
     6301 gaaatgtaat atgctcaact gccataataa tcgcttcatc gttttttgct gtaatcttta
     6361 agcaatttgg aaaagtagct ccgtcagcaa ttaatgaatg ataacgcatt acattgaaat
     6421 tttgaggcag tccttgaaaa ataccttcat tggtatggcg taactgtgta gtatgtccgt
     6481 gtacaggatg atagccgtga atgatatttc caccaaaata agacacgata cattgaaatc
     6541 ctaaacatac acctagtata ggtacacgct gataaaattg ttctaacact tcattcaaga
     6601 taggataatc atccggatta cccggcccag gtgaaataac aattgctttt ggcttcatat
     6661 taatgacgtc ttctatcagc agattatcaa taccaacaac ttgaactgtt agtttcgttt
     6721 gagtcttaat atagtctatt aaattatatg taaatgaatc attattatct atgactagaa
     6781 tcattgtata ctccgttcta aatgtgtttt atttttataa tatgtattgg atgtagctaa
     6841 aactttaaag gcattgtcat tatcctgacc ttgattttaa ctaatatatg gtatattcta
     6901 ttcatcgtac ataaatgaat atcagaggtt cctagctgaa accctctata aaaaactaga
     6961 cattgaaatt tcaaacattc gttggtaaac ctttaatata tattatagga gcaacccatt
     7021 tctttcgtct atcttttatg gagataggct ttttttattt tataaattaa aaacgggctt
     7081 atcaatgcca ttaacctctt tattaatatt attaatgata ggagcaatta caaaatggaa
     7141 agtgtattaa ataatgaaaa agccatcgtt gtctttagtg gtggtcaaga cagtacaaca
     7201 tgtctctttt atgcaaaaaa acatttcaaa gaagttgaac tcgtaacgtt taattatggt
     7261 caaagacatg atactgaaat tgaagtcgca aaacaaattg cacaagatca aggaatgaaa
     7321 catcacgttt tagatatgtc attattatca caacttactc caaacgcatt aacacaacat
     7381 gatatggaaa ttactaataa tgaagatggt atacctaata catttgttcc agctagaaat
     7441 ttacttttct tgtcgtttgc aggtgctcta gcttatcaaa ttggggctaa gcatattatt
     7501 acaggcgtat gtgaaacaga cttttcaggc tacccagact gtcgcgatag ttttataaaa
     7561 tcaatgaacg taacattaag cctagctatg gacaaagatt ttgttattca tactccttta
     7621 atgtggttaa acaaagcaga aacgtggaaa ttaagtgatg aactcgaagt tttagattat
     7681 attcgtacaa aaacattaac atgctataac ggtatcattg gggatggctg tggtgaatgt
     7741 ccagcttgtc atttacgtca acgtggacta aatcaatatc ttgaaagtaa aggagcgctt
     7801 taaaatgtta caacaaatct atcctagtac aacgcatcca tatcaattcg aattaaataa
     7861 agattttaat ttttcagctg cacatcacat tccgtgtgaa gaagcaggta tttgtcaaaa
     7921 tgtccatggt catacttact ttgttaattt aacaattgtc ggtgataaac tagatgacac
     7981 tggcttttta gtgaacttta gccatttgaa aaagatgata cacggtaaat ttgaccatca
     8041 actgttaaat aacttacctg cttttaaaaa caaaatccct tcaactgaaa tcgtagcgga
     8101 aacaatttat caaattgtta aagaaaattt ggcatcgctc gaacaccaac caaaatgtat
     8161 tcaagtattt gtaagagaaa caccaacaag ttatgtcgta tttagaccaa aggaacaggt
     8221 gtaactaatg gctaaaattc ctgtactcga aatattcggt ccaaccattc agggtgaagg
     8281 tcgagtaatc ggtagaaaaa cgatgtttgt aagaacagct ggatgtgatt atcgctgtag
     8341 ctggtgtgat tctgcattta cttgggatgg tagtgctaaa ggcgatataa aactcatgac
     8401 cgctgaagaa atttatgatg aattaaaacg aattggtggc gatttattta accacgttac
     8461 aatttctggt ggtaacccag cattaattaa aggtatccaa gagttagttg acttatttca
     8521 agataaaggt attttcagcg cactggagac acaaggcagt aaattccaac cttggatgac
     8581 acaaattgat gatttaacga ttagtccaaa accaccaagt tcaactatga cgcctgattt
     8641 aaaaaaatta gatgaagtga ttgcacaatg cgtaccttcc tcattaaact taaaagtagt
     8701 tgtattcgac gataaagatt atgattttgc caaaatgata caccaccgtt atccagatat
     8761 tcccttctat ttacaagttg gtaacccata tttatcagac agcgtagata atcataccga
     8821 aaagttgtta gaacgctacg agcagttggt agatttagtt atgcaaagta atgatataaa
     8881 tcacgtttat gtgttaccac aacttcatac attactttgg agtaataaaa aaggtgtata
     8941 aaggataact tattttactt tttacaagtc atatctaatg aataacaata tattaattga
     9001 tataatagtt gattgaagta taacgaacag gagtccatca tgattgtata tatattaatt
     9061 aatattgcta tcgtcgtact tataactgga ttcaatttat ataggcatca aatgcaacat
     9121 ttatcattaa gtgcaatgtt gttatctatt actataaacg cctttataaa tacattcata
     9181 attgataaat ataactttat tactctgtgt actataacga tgtttatcat ttggacgata
     9241 ctacagtttt acattgataa aaaattaaaa cctgtataca ttacagacca aaaatttatt
     9301 gctatcatat taacgattgt tgtcagttta acgcaacgtg ttaccgactt ttcaagtaca
     9361 caatcaatat atatgtctat tccattttta gcccctgcca ttttcatcat tggtggtatt
     9421 atgttattta tttcaacatt taatagctta gatgaatctg ctgaaaataa ta
//
LOCUS       DAPIDM010000103         9408 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000103 DAPIDM010000000
VERSION     DAPIDM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.103"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            530..1984
                     /locus_tag="RJE42_001988"
     CDS             530..1984
                     /locus_tag="RJE42_001988"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV5877397.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            2408..3055
                     /gene="cysE"
                     /locus_tag="RJE42_001989"
     CDS             2408..3055
                     /gene="cysE"
                     /locus_tag="RJE42_001989"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV5877398.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            3039..4439
                     /locus_tag="RJE42_001990"
     CDS             3039..4439
                     /locus_tag="RJE42_001990"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV5877399.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGA
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            4432..4836
                     /locus_tag="RJE42_001991"
     CDS             4432..4836
                     /locus_tag="RJE42_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDV5877400.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNEIITIVNER"
     gene            4844..5590
                     /gene="rlmB"
                     /locus_tag="RJE42_001992"
     CDS             4844..5590
                     /gene="rlmB"
                     /locus_tag="RJE42_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV5877401.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            5590..6114
                     /locus_tag="RJE42_001993"
     CDS             5590..6114
                     /locus_tag="RJE42_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDV5877402.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            6196..6765
                     /locus_tag="RJE42_001994"
     CDS             6196..6765
                     /locus_tag="RJE42_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDV5877403.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRCIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGKQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            6880..7023
                     /gene="rpmG"
                     /locus_tag="RJE42_001995"
     CDS             6880..7023
                     /gene="rpmG"
                     /locus_tag="RJE42_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV5877404.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            7079..7261
                     /gene="secE"
                     /locus_tag="RJE42_001996"
     CDS             7079..7261
                     /gene="secE"
                     /locus_tag="RJE42_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV5877405.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            7274..7822
                     /gene="nusG"
                     /locus_tag="RJE42_001997"
     CDS             7274..7822
                     /gene="nusG"
                     /locus_tag="RJE42_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV5877406.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            8003..8425
                     /gene="rplK"
                     /locus_tag="RJE42_001998"
     CDS             8003..8425
                     /gene="rplK"
                     /locus_tag="RJE42_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV5877407.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            8633..9325
                     /gene="rplA"
                     /locus_tag="RJE42_001999"
     CDS             8633..9325
                     /gene="rplA"
                     /locus_tag="RJE42_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV5877408.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
BASE COUNT         3533 a         1261 c         1844 g         2770 t
ORIGIN      
        1 atattaaaag gtatatgaaa tggcgaagtt tgtaatttga agtataacta taaatagatg
       61 caaaacatat cagttatcaa aaaatacaaa tagtgctaat atatcattga aacaagttca
      121 ttaagtgtta aattttagat attaataaag ttattcttat agcaatcaag atatacaaaa
      181 ttagaatcaa ttttaacgta tttgaatagt taatttgaag tttaatttta cagtacataa
      241 tattcatttt taagtcgttg aaatacgtat gtagtgcagt cttgattctt agggatagct
      301 tttttaaagt gttgaaaaaa caactgcttt ctatttaaaa tttaagcata ttacccaggg
      361 aattatgtta tgattaaaca atgaaattat tttaaggtca tgttaaaaag cattgtttcg
      421 ttttaacaat aagacgagtt acacatttgt tatgacggtc gaagatagca aatgaacaat
      481 ttatatgaac taacttgaaa atctgatatt taatttagga gtgaaaaaaa tgagcgatcg
      541 tataagagta agatatgcac caagtccaac tggttatctt catattggta atgcaagaac
      601 agcattattc aattacttgt atgctaaaca ttacaacgga gattttgtga ttcgaattga
      661 agatactgat aaaaaacgta atttagaaga tggagaaaca tcacaatttg ataatcttaa
      721 atggttagga ttagattggg atgagtctgt agataaagac aatggctacg gaccatatcg
      781 tcaatctgaa cgtcaacata tctaccaacc attaatagat cagttactag cagaagataa
      841 agcatataaa tgctatatga cagaagaaga attagaagct gaacgtgaag cgcaaatcgc
      901 tcgtggtgaa atgcctcgct atggtggtca acatgcgcat ttgactgaag aacaacgtca
      961 acaatttgaa gcagaaggac gccaaccatc aattcgtttc cgagtacctc aaaatcaaac
     1021 gtattcattt gatgatatgg ttaaagggaa tatttcattt gattcaaatg gtattggtga
     1081 ctgggttatc gttaaaaaag atggcattcc aacgtacaat tttgcagtag ctatagatga
     1141 tcattacatg gaaatttcag atgtcattcg tggtgatgat catatttcaa acacgcctaa
     1201 acaaattatg atttatgaag catttggctg ggagccacct cgttttggtc atatgtcatt
     1261 aattgttaat gaagaacgta aaaagttaag taaacgtgat gggcaaattt tacaatttat
     1321 tgagcaatat cgtgacttag gttatttacc tgaagcgtta tttaatttta ttgcgttatt
     1381 aggttggtct cctgaaggtg aagaagaaat cttttctaaa gaagaattta tcaaaatctt
     1441 tgatgaaaag cgtttatcaa aatcaccagc atttttcgat aagcaaaaat tagcatgggt
     1501 taataaccaa tatatgaaac aaaaagatac tgaaacagta ttccaattag cattacctca
     1561 tttaattaaa gcgaatttga ttcctgaggt gccgtcagaa gaggatttat cttggggacg
     1621 caaattaatt gcgctttatc aaaaagaaat gagttatgcc ggtgaaattg tacctttatc
     1681 agaaatgttc tttaaagaaa tgccagctct tggtgaggaa gaacaacaag tgatcaatgg
     1741 agagcaagta ccagagttaa tgacgcactt attcagtaaa ttagaagcac ttgaaccatt
     1801 tgaagcggct gaaattaaaa agacaattaa agaagttcaa aaagaaacag gaataaaagg
     1861 caagcaatta tttatgccta ttcgtgttgc tgtaacaggc caaatgcatg gtcctgaatt
     1921 accaaataca attgaagtac ttggtaaaga aaaagtgcta aaccgtttaa aacaatataa
     1981 gtaatgaaat agacaagatc aaggtagtat atacttgaat tttataacgt aaccacatat
     2041 gataaagttg tattaacaaa ttacaacaat atgaatagag tattttataa aaaacgaagg
     2101 attagtaatt aaatttatac gatgcagaga gtgtacggtt gctgtgagta caacgtagaa
     2161 attaatgaat gcaccttcgt aaaatgaatt aaatatataa tgagagtgat gagcattaag
     2221 ttgacttagt ttccttgata atttggaagc gcccgcaatt ttattaatgt aattcgctaa
     2281 attcagagtg gaaccgtgcg gaagcgcctc taacaataca atttgtatgt tagtggtgct
     2341 ttttttgata tttaatttcg caggtacttc aattatgtat ttacgtagtt caatcattga
     2401 ggaggaaatg atcttgttaa aaagaatgag agacgatata aaaatggtat ttgagcagga
     2461 tccagcggca cgttcaacat tagaagtgat tacaacgtat gcaggtttac atgcagtttg
     2521 gagtcatttg attgcacata agttatacaa ccaaaaaaaa tatgttgcag cacgcgcgat
     2581 atctcaaatt tcaagatttt tcacaggtat agaaatccat ccaggtgcta aaattggaaa
     2641 gcgtctattt atagatcatg gtatgggcgt tgtaatagga gaaacatgta caattggtga
     2701 taatgtgaca atctatcaag gcgtgacact tggtgggaca gggaaagaaa gagggaaaag
     2761 acacccagat ataggagaca atgttttaat agcagcgggt gcgaaagttt taggaaatat
     2821 taaaataaat tcaaatgtaa atattggtgc aaattcagtt gttttacaat cagttccaag
     2881 ttattcaacg gttgttggta taccaggaca tattgttaag caagatggtg ttcgagttgg
     2941 aaaaacattt gatcatcgcc atctacctga tccaatttat gaacaaatta agcatttaga
     3001 acgacaactt gaaaagacta ggaatggaga gattcaagat gattacatta tataatacgc
     3061 ttacacgtca aaaagaagtg ttcaagccaa tagaaccagg aaaagtaaaa atgtatgtgt
     3121 gtggtcctac tgtatataac tacattcata ttggtaacgc gagaccagca attaattatg
     3181 acgtagtgag acgttacttt gaataccaag gatataatgt agaatatgta tcaaatttta
     3241 cagatgtaga tgataaatta attaaacgtt ctcaagaatt aaatcagtct gttcccgaaa
     3301 ttgcagaaaa atatatcgct gcttttcatg aagatgttgg tgcgttaaat gttagaaaag
     3361 cgacttcaaa tccaagggta atggaccata tggatgacat tattcaattt attaaagatt
     3421 tggtggatca aggttatgca tatgaaagtg gtggcgatgt ttactttaga acacgtaaat
     3481 ttgaaggtta tggtaaatta agtcatcaat ccatagatga cttaaaagtg ggtgctcgta
     3541 tagatgcagg agagcataag gaagatgcac ttgattttac tttgtggaaa aaagcgaagc
     3601 ctggcgagat tagttgggat agcccatttg gtgaagggag accaggatgg catattgaat
     3661 gttctgtaat ggcatttcat gagctaggag ctacaattga tatacatgcg ggtggttcag
     3721 atttacaatt tccacatcat gaaaatgaaa tagcacaatc ggaagcacat aatcatgcgc
     3781 cattcgctaa ttattggatg cataatggtt ttattaatat tgataatgaa aaaatgagta
     3841 aatcactagg caactttatt ttagttcacg atattattaa agaagttgat ccagatgtat
     3901 taagattctt tatgattagc gtacattata gaagtccaat taactataat ctagaattgg
     3961 ttgaatcagc acgtagtgga ttagagcgta ttcgcaatag ttatcaatta attgaagagc
     4021 gcgcacaaat tgctactaat attgaaaatc aacagacata tattgatcaa attgatgcga
     4081 ttttaaatcg ttttgaaaca gttatgaatg atgattttaa tacagctaat gcaattacag
     4141 cttggtatga tttagcaaaa cttgcgaata aatatgtact agagaataca acatcaacag
     4201 aagtaattga taaatttaaa gcagtttatc aaattttcag cgatgtttta ggtgtaccgt
     4261 taaaatctaa aaatgcagat gaattattgg atgaagatgt tgaaaaatta atcgaagagc
     4321 gtaatgaagc aaggaaaaac aaagattttg cacgagcaga tgagattcga gacatgctga
     4381 aatcacaaaa cattatatta gaagacacac ctcaaggggt tagatttaaa cgtggataat
     4441 caacaagata atcacattaa attattgaat ccattgacct tagcatatat gggagacgca
     4501 gtcttagatc aatatgtacg tacgtatatc gttttaaagc ttaaaagtaa gcctaataaa
     4561 ctacatcaaa tgtctaaaaa atatgtatct gccaaaagtc aggcgcaaac gttagaatat
     4621 ttaatggagc aagaatggtt tacagacgaa gaaatggata ttttgaagcg agggcgtaac
     4681 gcgaaaagtc atactaaagc taaaaatact gatgttcaaa catatcgtaa aagttcagcg
     4741 atagaagcag tgataggttt tctttattta gaaaaaagag aagaacgatt agaggcatta
     4801 ttaaatgaaa taataacaat agtaaacgaa aggtagtgac aatgtggaag atacggttat
     4861 tgttggtagg catgctgtta gagaagcgat tattactggg catccgataa ataagatatt
     4921 gattcaagaa ggaattaaaa agcaacaaat taatgaaatt ttaaaaaatg caaaagatca
     4981 aaaaatcatt gttcaaactg taccaaaatc taaattagat tttttagcaa atgcaccaca
     5041 tcagggtgtt gcagcactta ttgcaccata tgaatatgct gacttcgatc aatttttaaa
     5101 acagcaaaaa gaaaaagaag gtttatcgac agtacttata ttagacggct tagaagaccc
     5161 gcataacttg ggatcaattt taagaacagc agatgcaacg ggagttgatg gtgttattat
     5221 tcctaaacgt cgttcagtta cactaacgca aacagttgca aaagcctcaa caggtgcaat
     5281 tgaacatgta ccagttattc gagtgacaaa tttagctaaa actatcgatg aactaaaaga
     5341 taatggcttt tgggtagctg gcactgaagc taataatgca acagattata gaaatcttga
     5401 agcggacatg tcattggcta ttgtaattgg cagcgaagga cagggtatga gtcgtctagt
     5461 aagtgataaa tgcgattttt atattaagat tccaatggtt ggacatgtaa acagtttgaa
     5521 tgcttcggtt gcagcaagtt taatgatgta cgaagtattt cgaaaaagac atgatgttgg
     5581 agaaatataa tgaaagaacg ttacttaatc attgatggat acaatatgat aggacaatca
     5641 ccaacgctaa gcgccattgc aaaagagaat ttagaagaag ctagaatgca attaatagat
     5701 gcaattgcaa attataatgc agttatttca gatgaaatta tttgtgtttt cgatgcttat
     5761 gaccaatcgg gtgttgaaag agaatacatg tatcatggcg ttaaaacgat ttttaccaag
     5821 gaaaaagaaa cagctgatag tttcatagaa cgttatgtct atgaacttta tgacaagcac
     5881 actaagcata ttacagttgt aacaagtgat atgagtgagc aacatgctat ctttggatca
     5941 ggtgcatata gaatatcatc tcgcgaaatg tggagagatt taaaagaaaa tgaaattgat
     6001 gtgagtaaat cattagatga tataagtgaa aacaagccaa gaactcgaat tccgttatct
     6061 tctgaaatcc ttgcagaatt tgaaaaaata cgaagaggac atcataagaa atgacatttc
     6121 cgtcatcttg aaattttgaa tgtaacaata ttaaactaac tttaaatttt tagatagaag
     6181 gggttagttt aatacttgaa atatgatttg acaactcaag acagtacaat caaacgtaac
     6241 aatgcaataa atgataaaga cttcgaaaag ttagtaatgg atctacaacc attaattatt
     6301 cgatgcatca aaacatttgg atttaatcat tatgatttag aagacttata tcaagaaata
     6361 cttatacgga tgtataggtc ggtccaaaca tttgatttta gtggaaagca gcctttcaca
     6421 aattatgttc agtgtttaat tacgtctgta aagtatgatt atttgagaaa atatttagct
     6481 acaaataaaa gaatggataa tttgattaat gaatatagag ttacgtatcc atgtgcaata
     6541 aagcgttatg atgttgaaaa caattatttg aataaattag caattaaaga gttgattcgt
     6601 cagtttaagt atttgagtgc atttgaaaaa gatgtcatgt atttaatgtg tgaacaatat
     6661 aagccgagag aaattgctca actgatgcat gtaaaagaga aagtgattta taatgccata
     6721 caacgatgta aaaataaaat aaaacgttat ttcaaaatga tttgaaaagc gccctaggac
     6781 gtgaattgaa ttagaacgtg ttacttcctg atggtttgac atttgttata aattttatgt
     6841 atagtatact ggtattataa tgaataaagg tgaattattg tgaggaaaat acctttaaat
     6901 tgtgaagctt gtggcaatag aaattataat gttcctaagc aagaaggctc ggcaacaaga
     6961 ttaaccttaa agaaatattg tccaaaatgt aacgcgcaca caattcataa agaatcgaaa
     7021 taaatacatt cgaaataata ctttgataat atgttcaaag gatttggagg ttgagcagat
     7081 ggctaaaaaa gaaagtttct ttaaaggcgt taagtctgaa atggaaaaaa caagttggcc
     7141 gacgaaagaa gagctattta aatatactgt aattgtagtt tctactgtta tattcttctt
     7201 agtctttttc tatgccttag atttaggaat tacagcattg aaaaatttat tatttggtta
     7261 gaggagtgaa gacatgtctg aagaagttgg cgcaaagcgt tggtatgcag tgcatacata
     7321 ttctggatat gaaaataaag ttaaaaagaa tttagaaaaa agagtagaat ctatgaatat
     7381 gactgaacaa atctttagag tagtcatacc ggaagaagaa gaaactcaag taaaagatgg
     7441 caaagctaaa acgactgtta aaaaaacatt ccctggatat gttttagtgg aattaatcat
     7501 gacagatgaa tcatggtatg tggtaagaaa tacaccaggc gttactggtt ttgtaggttc
     7561 tgcaggtgca gggtctaagc caaatccatt gttaccagaa gaagttcgct tcatcttaaa
     7621 acaaatgggt cttaaagaaa agactatcga tgttgaactc gaagttggcg agcaagttcg
     7681 tattaaatca ggtccatttg cgaatcaagt tggtgaagtt caagaaattg aaacagataa
     7741 gtttaagcta acagtattag tagatatgtt tggccgagaa acaccagtag aagttgaatt
     7801 cgatcaaata gaaaagcttt aattaacaat taaagttatt aaactaacca aaagataaaa
     7861 aagagtattg attttttaat tagaaaagtg ttaaaattat gtggtcgcgc ttttagagcg
     7921 cccatttcgt cacgaaatgt taagagtggg agggcaaaac tgagccctgt gaccacatca
     7981 cgatatcaag gaggtgcaca tcgtggctaa aaaagtagat aaagttgtta aattacaaat
     8041 tcctgcaggt aaagcgaatc cagcaccacc agttggtcca gcattaggtc aagcaggtgt
     8101 gaacatcatg ggattctgta aagagttcaa tgcacgtact caagatcaag caggtttaat
     8161 tattccggta gaaatcagtg tttatgaaga tcgttcattt acatttatta caaaaactcc
     8221 accggctcca gtattactta aaaaagcagc tggtattgaa aaaggttcag gcgaaccaaa
     8281 caaaactaaa gttgctacag taactaaaga tcaagtacgc gaaattgcta acagcaaaat
     8341 gcaagactta aacgctgctg acgaagaagc agctatgcgt attatcgaag gtactgcacg
     8401 tagtatgggt atcgttgtag aataatttta cgaatattaa atttgattac atgatttaaa
     8461 cgatgaagca gataacagag ataataatga tgaattataa atataatctg aatgactaga
     8521 ttaatgattg atttattcat aagattaatt cttctgttgt ctgctcttaa cttgcatcta
     8581 gcaagtaatg tgggaggaaa ttccgctaaa accactaaag gaggaactat aaatggctaa
     8641 aaaaggtaaa aagtatcaag aagcagctag taaagttgac cgtactcagc actacagtgt
     8701 tgaagaagca attaaattag ctaaagaaac aagcattgct aactttgacg cttctgttga
     8761 agttgcattc cgtttaggaa ttgatacacg taaaaatgac caacaaatcc gtggtgcagt
     8821 tgtattacca aacggaactg gtaaatcaca aagtgtatta gtattcgcta aaggtgacaa
     8881 aattgctgaa gctgaagcag caggtgctga ctatgtaggt gaagcagaat acgttcaaaa
     8941 aatccaacaa ggttggttcg acttcgatgt agtagttgct acaccagaca tgatgggcga
     9001 agttggtaaa ttaggtcgtg tattaggacc aaaaggttta atgccaaacc ctaaaactgg
     9061 aactgtaaca atggatgtta aaaaagctgt tgaagaaatc aaagctggta aagtagaata
     9121 ccgtgctgaa aaagctggta tcgtacatgc atcaattggt aaagtttcat ttactgatga
     9181 acaattaatt gaaaacttca atactttaca agatgtatta gctaaagcta aaccatcatc
     9241 tgctaaaggt acatacttca aatctgttgc tgtaactaca acaatgggtc ctggagttaa
     9301 aattgatact gcaagtttta aataataaat gatataaaca attacaggct gaaagaaata
     9361 tctttcagtc tgtaaaaata tattgacaat aagtaatttc caagttat
//
LOCUS       DAPIDM010000104         9303 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000104 DAPIDM010000000
VERSION     DAPIDM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.104"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            14..844
                     /locus_tag="RJE42_002000"
     CDS             14..844
                     /locus_tag="RJE42_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDV5877409.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(908..1126)
                     /locus_tag="RJE42_002001"
     CDS             complement(908..1126)
                     /locus_tag="RJE42_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877410.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(1140..1721)
                     /locus_tag="RJE42_002002"
     CDS             complement(1140..1721)
                     /locus_tag="RJE42_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877411.1"
                     /translation="MKKLVSIVGATLLLTGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            1826..2887
                     /locus_tag="RJE42_002003"
     CDS             1826..2887
                     /locus_tag="RJE42_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDV5877412.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            2913..3470
                     /gene="efp"
                     /locus_tag="RJE42_002004"
     CDS             2913..3470
                     /gene="efp"
                     /locus_tag="RJE42_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV5877413.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            3943..4407
                     /gene="accB"
                     /locus_tag="RJE42_002005"
     CDS             3943..4407
                     /gene="accB"
                     /locus_tag="RJE42_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV5877414.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDNNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            4407..5762
                     /gene="accC"
                     /locus_tag="RJE42_002006"
     CDS             4407..5762
                     /gene="accC"
                     /locus_tag="RJE42_002006"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV5877415.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNEIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            5777..6139
                     /locus_tag="RJE42_002007"
     CDS             5777..6139
                     /locus_tag="RJE42_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDV5877416.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            6199..6588
                     /gene="nusB"
                     /locus_tag="RJE42_002008"
     CDS             6199..6588
                     /gene="nusB"
                     /locus_tag="RJE42_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV5877417.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            6605..7942
                     /locus_tag="RJE42_002009"
     CDS             6605..7942
                     /locus_tag="RJE42_002009"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV5877418.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDNANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDVTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            7935..8165
                     /locus_tag="RJE42_002010"
     CDS             7935..8165
                     /locus_tag="RJE42_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV5877419.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            8143..9024
                     /locus_tag="RJE42_002011"
     CDS             8143..9024
                     /locus_tag="RJE42_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDV5877420.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTKHELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPINLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELMQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         3458 a         1266 c         1711 g         2868 t
ORIGIN      
        1 ggagttcttg aatatgactg aaacttggaa ttttattaat acaggaagta aagacccata
       61 ttataatatg gcaatggatg aagcgttatt aaattttgtc tctagaggtg aaattgatcc
      121 agttatacgt ttttacacat ggaatcctgc aacattatca atagggtatt tccaaaggct
      181 acaaaaagaa attgatattg ataaggttaa agagaaaggt tttggtctcg taaggcgtca
      241 aactggtggc cgtggggtac ttcatgataa agagctaact tacagtgtga ttgtgcctga
      301 atcgcatccc aatatgcctt ctactgttac agaagcgtac agagtgattt cacaaggatt
      361 attagaagga tttaaaaact taggatttga tacttatttt gcggtaccta aaacgcctga
      421 agagcgtcaa aaattaaaac aacctcgaag ttcagtgtgt tttgatgcac caagttggta
      481 tgagctagtt gttgaaggcc gtaagattgc tgggagtgct caaacgagac aaaagggtgt
      541 aatcctccaa cacggttcga ttttacaaga tattgatatt gatgaattgt ttgatatgtt
      601 tatttataaa aatgaaagat taaagttaaa aatgaaagag gcattcgttg aaaaagcagt
      661 tgccatcaat gatatttcag atgaacacat tactattagt caaatggaag aagctttcga
      721 gaaaggtttt aagaaaggtt taaatataga attaaagcca cttgaattaa cagaagctca
      781 attggcagaa gtggaagaat taactgaaaa atatcgttca gatgaatgga tgtttagaaa
      841 gtagttttta gaataaaaaa tggaagcacg tagactattt tacctacaag tgcttccatt
      901 tttttatcta ttttcttctt tgatttcttt tatacttacg cattgctttg cgatatttgc
      961 gttggtcttc agttaagatg aagaaataat aaatcagata aataatacca gctattacag
     1021 caaagctgac taacattctg acgattgaaa aaataaaggc atctagattc attactaatc
     1081 caatcgcagc aataataagt acaagataaa atattatatt acgcattaaa agactcctat
     1141 catgattttg catttaagtt taattttgac aacgcttgtt gtccttttgt aagttgtttt
     1201 ttatctttat tttgatagcc atttttaata tcatcaaatg catgattcaa atcagagttt
     1261 aatttatcga tatttttaga cgttttttta tcatcatcac taagtgataa accatcgata
     1321 gcatctttgt atgaagtgtg agcactttga atatcattag aaatttgatt taatttcgat
     1381 tgaacattct tatcgccttt gtttttactt acatctttct caatatcgtt ataggatttc
     1441 aaagctttag caactttgtc gtagtatgtt gatgtaacat tcatcaattt aattttcgta
     1501 ttattagatg cagttttttt actgttttct ttatcctttt caagcccttt aattttagat
     1561 ttagaatcac taatttgttg attaagttct tgaatatcta gtttgagttg atgattatct
     1621 tctcttaaat ctgttgtttt ttcttctaat ggtgctaaat tttgtgatcc acatccagtt
     1681 aacaataatg ttgcgccaac aattgaaacc aattttttca ttactatctc cctaaaatca
     1741 atatctatta ttttttatcc ttattatgat attattttaa tgtataaagt tgaaatgcac
     1801 aaacgtttca ctaggaggta ataagatgag cagaataaca caagtgcatc gtattttaga
     1861 acaaaaacat ttagatgcga ttatcattct ttcggattac aatagaagat atttatcagg
     1921 ttttactggt accagtggag cacttatcat atctaaagat aaacaatatt tgataacaga
     1981 ctttagatat attgatcaag caactaagca ggcgccaaat tatgaaatta ttaatcgtaa
     2041 atctactatt attggtgaga ttaaagaatt gctacaccaa gaaaattttg aaaatgtagg
     2101 ttttgagggg caccatgtaa gttatgatac ataccttgaa ttaaataaaa gccgtatatc
     2161 attaataagc atttctaata ctgtagataa aattagagac gtcaaagatg ctgacgaaat
     2221 tgctttaatt caaaaagcag ctaatattgt tgatgaaaca tatgaatata ttttaactgt
     2281 tgtaaaagca ggtatgactg aaaaagaatt aaaggcaata ttagaaagta aaatgctaga
     2341 attaggagca gatggaccat cattcgatac gattgtagca tctggtcata gaggtgcatt
     2401 accacatggt gttgcaagtg ataaaattat tgaaaaaggc gacatgatta cattagattt
     2461 tggtgcgtat tataacggct attgttcaga tattactaga acatttgcta ttggagaacc
     2521 agatcctaag ctgaaagaaa tatatcaaat agtacttgaa tcacaaatga aagcaattaa
     2581 tgagattaga cctggcatga ctggtgcaga agctgatgcc atttcaagaa actatttaga
     2641 gtcaaaaggg tatggaaaag aatttggaca ttcactagga catggtattg gtttagaaat
     2701 ccatgaaggg ccaatgctgg ctcgtacgat acaagataaa cttcaagtta acaactgtgt
     2761 tacagtagaa cctggtgttt atatagaagg tttgggcggt ataagaatag aagatgatat
     2821 tttaattaca gaaaatggtt gtcaagtctt tactaaatgc acaaaagacc ttatagtttt
     2881 aacataagcg tgtaaaatga ggaggaaact gaatgatttc ggttaatgat tttaaaacag
     2941 gtttaacaat ttctgttgat aacgctattt ggaaagttat agacttccaa catgtaaagc
     3001 ctggtaaagg ttcagcattc gttcgttcaa aattacgtaa tttaagaact ggtgcaattc
     3061 aagagaaaac gtttagagct ggtgaaaaag ttgaaccagc aatgattgaa aatcgtcgta
     3121 tgcaatattt atatgctgac ggagataatc atgtatttat ggataatgaa agctttgaac
     3181 aaacagaact ttcaagtgat tacttaaaag aagaattgaa ttacttaaaa gaaggtatgg
     3241 aagtacaaat tcaaacatac gagggtgaaa ctatcggtgt tgaattacct aaaactgttg
     3301 aattaacagt aactgaaaca gaacctggta ttaaaggtga tactgcaact ggtgctacta
     3361 aatcggcaac tgttgaaaca ggttatacat taaatgtacc tttatttgta aacgaaggtg
     3421 acgttttaat tatcaacact ggtgatggaa gctacatttc aagaggataa tctctaattt
     3481 gttaacaaaa agcttgtatt cactatactg atttaacgta agatattcca aataagtctc
     3541 ataaagctat tgcctaaaat gattataggt tatatgctga tatgaggctt tttattttta
     3601 aaataaattt taaaatataa aacgaatcgt ctaattggta gtaaaaaaac atagacatga
     3661 gtaagggtat atatctacat atcatattgt tgaggataag aaataatagc taataaaaat
     3721 aacattgata tgtttaactg aatgcataaa atttttaaca agagttaaga tgagtcaatg
     3781 ttaagtaata aatccacaag tgtttttaat ttaaaatgtg gttttattat aactttggga
     3841 tagacttata atgtcgcctg cgatatgaac atgcttgaat tgaagagttg tctcaagtaa
     3901 aatagacggg tagatgaaaa caaactgaag gagtcagtaa taatgaactt taaagaaatc
     3961 aaagaattaa ttgaaattct ggataaatca actttaacgg aaatcaatat tgaagatact
     4021 aaaggcaaag tgacgcttaa gaaagaaaaa gaaactgaga ttatcacgcc acaaatctca
     4081 caaatgccag ttgaagctgc ggcaatgcct atgcctcaag tacaatcaac tgataacaat
     4141 aaaactgaag ctccaaagcc aacttcagat aatcacaaaa caattaatgc acctatggta
     4201 ggtacatttt acaaatcgcc atctccagac gaagaagcct atgtgcaagt tggggacact
     4261 gtttcaaatg aaacaacagt gtgtatttta gaagcaatga aactatttaa tgaaattcaa
     4321 gcagaaattt caggtgaaat tgttgagatc ttagtagaag acggacaaat ggtagagtat
     4381 ggccaaccgt tatttaaggt gaaataatga aaaaggtttt aattgcaaac cgcggtgaaa
     4441 tcgcagttag gattattcgc gcttgtcgtg atttaggcat ccaaactgtt gcaatctatt
     4501 ctgaagggga taaagatgcg ctacatactc aaattgctga tgaagcatat tgcgtaggtc
     4561 ccactttgtc taaagattca tatttaaata ttccgaacat cttatctatt gcaacttcta
     4621 caggttgtga tggcgttcat ccgggttatg gctttttagc tgaaaatgct gactttgcag
     4681 aattatgcga agcatgccaa ttgaagttca ttggaccaag ttatcaatct atccaaaaaa
     4741 tgggtatcaa agatgttgct aaggcagaaa tgattaaagc caatgttcca gttgttcctg
     4801 gtagtgacgg tttaatgaaa gacgtctcag aagctaagaa aatcgctaaa aaaattggtt
     4861 atccggtcat cattaaagct actgctggtg gtggcggaaa aggtatccgt gttgctcgtg
     4921 atgaaaaaga acttgaaact ggcttccgaa tgacagaaca agaagcacaa actgcatttg
     4981 gtaatggtgg actttatatg gagaaattca tcgaaaactt ccgccatatt gaaatccaaa
     5041 ttgttgggga cagctatggt aatgtaattc atttaggaga acgtgattgt acaattcaaa
     5101 gacgtatgca gaaattagtg gaagaagcac cttccccaat tttagatgat gaaacacgtc
     5161 gtgaaatggg aaatgccgca gtgcgtgcag cgaaagctgt aaattatgaa aatgcgggaa
     5221 caattgagtt tatatatgat ttaaatgata ataaatttta ttttatggaa atgaatacac
     5281 gtattcaagt agaacatcct gtaactgaaa tggtaacagg aattgattta gttaaattac
     5341 aattacaagt tgctatgggc gacgtgttac cgtataaaca agaagatatt aaattaacag
     5401 gacacgcaat tgaatttaga attaatgctg aaaatcctta caagaacttt atgccatcac
     5461 caggtaaaat tgagcaatat cttgcaccag gtggatatgg tgttcgaata gagtcggcat
     5521 gttatactaa ttatacgata ccgccatatt atgattcgat ggtagcgaaa ttaatcatac
     5581 atgaaccgac acgagatgaa gcgattatgg ctggcattcg tgcattaagt gaatttgtgg
     5641 ttcttggtat tgatacaact attccattcc atattaaatt attgaataac gaaatattta
     5701 gaagcggtaa atttaataca aactttttag agcaaaatag cattatgaat gatgaaggtt
     5761 aataggaggt caatccatgg tcaaagtaac tgattattca aattcaaaat taggtaaagt
     5821 agaaatagcg ccagaagtgc tatctgttat tgcaagtata gctacttcgg aagtcgaagg
     5881 catcactggc cattttgctg aattaaaaga aacaaattta gaaaaagtta gtcgtaaaaa
     5941 tttaagccgt gatttaaaaa tcgagagtaa agaagatggc atatatatag atgtatattg
     6001 tgcattaaaa catggtgtta atatttcaaa aactgcaaac aaaattcaaa cgtcaatttt
     6061 taattcaatt tctaatatga cagcgataga acctaagcaa attaatattc acattacaca
     6121 aatcgttatt gaaaagtaat gtcataccta attcagtaat taaataaaga aaaatacaaa
     6181 cgtttgaagg agttaaaaat gagtcgtaaa gaatcccgag tgcaagcttt tcaaacttta
     6241 tttcaattag aaatgaagga cagtgattta acgataaatg aagcgataag ctttattaaa
     6301 gacgataatc cagatttaga cttcgaattt attcattggc tagtttctgg cgttaaagat
     6361 cacgaacctg tattagacga gacaattagt ccttatttaa aagattggac tattgcacgt
     6421 ttattaaaaa cggatcgtat tattttaaga atggcaacat atgaaatatt acacagtgat
     6481 acacctgcta aagtcgtaat gaatgaagca gtcgaattaa caaaacaatt cagtgatgat
     6541 gatcattata aatttataaa tggtgtattg agtaatataa aaaaataaaa ttgagtgatg
     6601 ttatatgtca gattatttaa gtgtttcagc tttaacgaaa tatattaaat ataaatttga
     6661 tcaagatcca catctccaat ctgtattgat taaaggtgag ctttcaaatt tcaaaaagca
     6721 ttcaagtggt catttatact ttaatgtgaa agataaagaa agcgttataa gtgccatgat
     6781 gttcaaaggt agtgcttcta aattaaactt cgaacctaaa gaaggagatg aagtcttatt
     6841 agaagcacgt gtttctgttt ttgaacgtcg tggaaactat caaatttatg taaataaaat
     6901 gcaattagat ggtataggga acttatatca aaaattagaa gcattaaaga aaaaattaac
     6961 tgaagaaggt tgttttgata atgcgaataa gaaatcaata cctaagtttc ctaaaaaaat
     7021 tgcagtttta acagcgagta caggtgccgc aattcgagat atccattcaa cgataaatag
     7081 tcgctttcca ttagctgaac aaatacaaat tagtactttg gttcaaggtg aaaaagcaaa
     7141 agacgacatt attgaaaaaa ttgaatatgc agatagttta ggtgtagata ccattattgt
     7201 aggtcgaggt ggtggatcca tagaagattt atggaatttc aacgaagaag ctgtcgtccg
     7261 tgcaatatat aattgtaaga caccgattat atcagcagta ggtcatgaaa cagacgttac
     7321 attaagtgat tttgctgcag acatcagagc ggcgactcca actcaagctg ctgttattgc
     7381 aacaccagac caatatgaat tgctgcaaca aatacagcaa tatcagttta cattgactcg
     7441 tttcatcaag aaacatttag aacaacaacg taaacatgtt gaacatttgt catcgtatta
     7501 caagtttaaa caaccaactt tattgtatga tcaacagata caacgtcgag atgatttaga
     7561 aaagagactg aaacaacaaa ttcaggcaac atttgaacaa cagagacatc gattaatgct
     7621 gttgcaacaa cgctataatt taaaagcgtt actaagtagt gttaatcaag aacaacaaaa
     7681 taatcttcaa ttgacaaacc aacttgttaa attattgaat tctaagatat taagttataa
     7741 aaatgatttg aaaaataaag tagaaaactt aaacaattta agtccaacta atacaatgtt
     7801 gcgtggatat gcaattgtta ataaaaaaga cgaagtcatt acgagtacta aagatttaac
     7861 agaaaatgat caattgacgt taacaatgaa agatggctta gtagatgcaa aagttacgaa
     7921 agtaaggtgt aataatgact aaagaaacgc aaagttttga agaaatgatg caagaattag
     7981 agcaaattgt tcaaaaatta gataatgaaa cagtatcttt agaggaatca ttagatttat
     8041 accaacgtgg tatgaaacta tcagcagctt gtgacacaac tttaaaaaat gccgaaaaaa
     8101 aggtgaatga cttaataaaa gaagaagctg aggatgtaaa aaatgacgaa tctaccgatg
     8161 aataaattaa tagatgaagt caataatgaa ttatcggttg cgataaataa atcagtaatg
     8221 gatactcagc tagaagaaag tatgttgtat tcattaaatg ctggaggtaa acgcatccga
     8281 ccagttctgt tattacttac tttagattca ctaaatacca agcatgagtt aggtatgaag
     8341 agcgcaattg cactagaaat gattcataca tattcactta ttcatgatga cctaccagcg
     8401 atggataatg atgattatcg acgagggaaa ttaacaaatc ataaagtata tggtgagtgg
     8461 acggcgatat tagcaggtga tgctttatta actaaagcat ttgaacttat ttcaagtgat
     8521 gatagattaa ctgatgaagt aaaaataaaa gttctacaac ggctgtcaat agcaagtggt
     8581 catgttggaa tggttggcgg tcaaatgtta gatatgcaaa gcgaaggcca accaattaat
     8641 cttgaaactt tggaaatgat acacaaaaca aaaacaggag cactattaac ttttgcggtt
     8701 atgagtgcag cagatatcgc taatgtcgat gatgcaacta aagaacattt agaaagttat
     8761 agttatcatt taggtatgat gtttcagatt aaagatgatt tattagactg ctatggtgat
     8821 gaagcgaagt taggtaaaaa agtgggcagc gatcttgaaa ataataaaag tacatacgtg
     8881 agtttattag gaaaagatgg cgcagaagat aaattgactt atcatagaga cgcagcagtg
     8941 gatgaactaa tgcaaattga tgaacaattc aatacaaaac acttattaga aatcgttgat
     9001 ttattttata gcagggatca ctaaaagcaa atatatcgat tagcaacaat tgttaagttt
     9061 gaaatgtctc aaaaggcata ttcaactgac gttgtttgtt aatcgatttt tctcatgttg
     9121 aaacttaaaa ttatgttaaa gactttcata taaaaataga attttcaatt tctatacgac
     9181 aatattcgca tctgtgatat caatctgatt ttaattaaat atgttcaaac aattgacgct
     9241 tattaaatct aatgattagc aagtttatta cacatatatt catttttata catattttaa
     9301 gca
//
LOCUS       DAPIDM010000105         9186 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000105 DAPIDM010000000
VERSION     DAPIDM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.105"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            136..933
                     /locus_tag="RJE42_002012"
     CDS             136..933
                     /locus_tag="RJE42_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877421.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            969..1973
                     /locus_tag="RJE42_002013"
     CDS             969..1973
                     /locus_tag="RJE42_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV5877422.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGLLVLVADMIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            1970..2986
                     /locus_tag="RJE42_002014"
     CDS             1970..2986
                     /locus_tag="RJE42_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV5877423.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIILQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            3220..4188
                     /gene="dhaK"
                     /locus_tag="RJE42_002015"
     CDS             3220..4188
                     /gene="dhaK"
                     /locus_tag="RJE42_002015"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV5877424.1"
                     /translation="MMKKLINKKETFLTDMIEGMLIAHPELEAVSNTVIVKKAKKEQG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVETVIVRDDIAVTNEAQRRGVAGTVFVHKLAGYLA
                     EKGHSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIVHIVGTLLDELYKEVTANEVIVMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            4230..4814
                     /gene="dhaL"
                     /locus_tag="RJE42_002016"
     CDS             4230..4814
                     /gene="dhaL"
                     /locus_tag="RJE42_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDV5877425.1"
                     /translation="MKVNDMKVRLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFIKL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            4807..5170
                     /locus_tag="RJE42_002017"
                     /pseudo
     CDS             4807..5170
                     /locus_tag="RJE42_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
     gene            5286..5783
                     /locus_tag="RJE42_002018"
     CDS             5286..5783
                     /locus_tag="RJE42_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877426.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            6239..7306
                     /locus_tag="RJE42_002019"
     CDS             6239..7306
                     /locus_tag="RJE42_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877427.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            7457..8500
                     /locus_tag="RJE42_002020"
     CDS             7457..8500
                     /locus_tag="RJE42_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV5877428.1"
                     /translation="MNKDNKWTMITALFIAVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVVIGG
                     DSAGAQLASQFTAIQTNNQLRNAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELKVPVDTLFYDGTHHLHHQYQFHLNKPESVDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            8830..>9186
                     /locus_tag="RJE42_002021"
     CDS             8830..>9186
                     /locus_tag="RJE42_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877429.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQY"
BASE COUNT         3174 a         1255 c         1742 g         3015 t
ORIGIN      
        1 gtcaataaga ttgaaaaatg ttataatgat gttcatgata atcattatca attgggatgc
       61 ctttgaaaat tgataattta aaaatagaaa ttatttttta taaacagaaa gaattttatt
      121 gaaagtaggg aaattatgaa tcgtttgcat ggacaacaag ttaaaattgg ttacggggat
      181 aacacgatta taaataaatt agatgttgaa ataccagatg gcaaagtgac gtcaatcatt
      241 ggtcctaacg gctgcgggaa atctactttg ctaaaggcat tgtcacgttt attggcagtt
      301 aaagaaggcg aagtattttt agatggtgaa aatattcata cacaatctac gaaagagatt
      361 gcaaaaaaaa tagctatttt acctcaatca cctgaagtag cagatggctt aactgttgga
      421 gaattagttt catatggtcg ttttccacat caaaaaggat ttggtagatt aactgctgag
      481 gataagaaag aaattgattg ggcaatggaa gttacaggaa ctgatacatt ccgacaccgt
      541 tcaatcaatg atttaagtgg tggtcaaaga caacgtgttt ggattgcaat ggcattagca
      601 caaagaactg atattatctt tttagacgaa ccaaccacat atttagatat ctgtcatcaa
      661 ttagaaatac tagaattagt tcagaagcta aatcaggaac aaggttgtac aattgtcatg
      721 gttcttcatg atatcaacca agcgattcgt ttctcagatc atcttattgc gatgaaagaa
      781 ggcgatatca tcgctacagg ttcaacagaa gacgtattaa cacaggaaat tttagaaaaa
      841 gtttttaata ttgatgttgt tttaagtaaa gatcctaaaa ctggaaaacc tttactggta
      901 acttatgact tatgtcgcag agcttattct taattaagta agttaatatg ataaaaagga
      961 caattaacat gacaaataga gagaatccaa cgccattgaa gtttttatcc tatattatag
     1021 gtttaagtat gatactacta atcacactat ttatttctac attaataggt gacgccaaaa
     1081 ttcaagcctc tacaattata gaggctattt ttaattataa tcctagcaat caacagcaaa
     1141 acatcatcaa tgagattagg attcccagaa atatagcagc agtaattgtg ggtatggcgc
     1201 ttgcagtttc tggtgcgatt atacaaggtg ttactcgtaa tggtcttgct gatccggcgc
     1261 tcataggttt aaattcaggt gcttcatttg ctttagcatt aacatatgca gttttaccaa
     1321 acacttcatt tttaatattg atgtttgctg gatttttagg tgctattcta ggaggtgcta
     1381 ttgtattaat gataggccga tctagacgtg atggatttaa tccgatgcgt attattttag
     1441 cgggtgcagc agtaagtgct atgttaacag cgctaagtca aggtattgca ttagctttta
     1501 gactaaatca aacagtaaca ttttggactg ctggaggcgt ttcaggcaca acgtggtcac
     1561 accttaagtg ggcaattcca ttaattggta ttgcgttatt cattatatta acaattagta
     1621 aacaacttac cattttaaat cttggtgaat cattagctaa aggtttaggt caaaatgtaa
     1681 caatgatcag aggcatatgt ttaattattg ctatgattct agcaggtata gcagttgcta
     1741 tcgctggaca agttgcattt gtaggtttga tggtacctca tatagcaaga tttttaattg
     1801 gaactgatta tgctaaaatt ctaccattaa cagccttgtt aggtgggtta ctcgtgcttg
     1861 ttgccgatat gatagcacga tatttaggag aagcgcctgt tggtgcaatc atttcattta
     1921 tcggtgttcc ttacttttta tatttagtta aaaaaggagg acgctcaata tgattagttc
     1981 aaataataaa cgcagacaat ggatagcact ggctgttttt agcattctac tatttctagg
     2041 ttgtacttgg agtattactt caggtgaata caacatacct gtagaaagat ttttcaaaac
     2101 tttaattgga caaggtgatg ccattgatga attaatctta ttagatttca gattacctcg
     2161 gatgatgatt actattttgg caggtgcagc gcttagtatt agtggtgcaa tagtgcaaag
     2221 tgtcacgaaa aatccaatag ctgaaccagg tatattaggt attaacgcag gtggcggatt
     2281 tgcaatcgca ttatttattg caattggtaa aattaatgct gacaactttg tttatgtact
     2341 gccgttaata agtatactag gtggtatcac cgctgcattg attattttta ttttcagttt
     2401 taataaaaat gaaggtgtta cacctgcgag tatggtatta ataggtgtag gtttacaaac
     2461 agcattatat ggtggctcaa ttacaattat gtcaaaattt gatgataagc aatctgattt
     2521 catcgctgct tggtttgcag gtaatatttg gggtgacgaa tggccatttg tcattgcatt
     2581 tttaccgtgg gtgttgatta ttattcctta cttactattt aaatcgaata cactaaatat
     2641 tattcatacg ggtgataata ttgcacgagg tctaggtgta aggttaagca gagaacgttt
     2701 aatattattc tttatcgcag tgatgttatc atctgctgct gtagcagtag caggttcaat
     2761 ttcgtttatc ggattaatgg gcccgcatat tgccaaacgt atcgttggac cacgtcacca
     2821 gttgttttta ccaattgcca ttttagtagg ggcatgttta cttgttatag ctgatacaat
     2881 tggcaaaatt atattacaac caggtggggt tccagcaggt atagtcgtag caattattgg
     2941 cgcaccgtat ttcttatatt taatgtacaa aacgaaaaac gtatagtgtc aaaggacaca
     3001 acttattgct atgaaaagca ctttattata aggcttttca tagcattttt tatttaatga
     3061 gtcactcaag actatttaca ttttcaataa tgaaccatta agttatcaag acggtcttat
     3121 cataaatata tttgataacg gtatcaggtt aaccctttat gatagcgcat tcatttattc
     3181 tgttttatac tatgactgat aataccaagg aggtacaaca tgatgaaaaa gttaatcaat
     3241 aaaaaagaaa catttttaac tgatatgata gagggcatgt taattgcgca cccagagcta
     3301 gaagcggttt ccaatactgt tatagtaaaa aaagctaaaa aagaacaagg tgtagcaata
     3361 gtctctggag gtggaagcgg acatgaacct gcgcatgctg gatttgtagc tgaaggtatg
     3421 ctagatgcag cggtttgtgg cgaagtattt acatcaccta caccagataa aatattagaa
     3481 gctattaaag cagtagatac tggtgatggt gtattactag ttgtgaaaaa ctatgcaggt
     3541 gacgtgatga attttgaaat ggcacaagag cttgcagaaa tggaaggtat aaatgttgaa
     3601 acagttattg ttcgtgacga cattgctgtg acaaatgaag cacaacgtcg tggtgttgca
     3661 ggaacagtgt ttgttcataa gcttgccggt tatcttgctg aaaaaggtca ctcattaaca
     3721 gagattaaat cgcgtgtaga agcgttgtta cctgaaatta aaagtattgg tatggcaatt
     3781 gagccaccgc ttgttccaac tactggaaaa tatggctttg atattgaaga cgacaaaatg
     3841 gaaatcggta ttggtataca tggtgaaaaa ggtattcata gagaagaagt aaaggatatt
     3901 gttcatattg ttggaacatt attagacgaa ttgtataaag aagttactgc caatgaagtc
     3961 atagtaatgg taaatggtat gggtggtacg ccgttatctg aattaaatat cgtaactaaa
     4021 tatattcaac aaaatttggc tgcaagaacg gttaatgttg ctaaatggtt tgttggtgat
     4081 tatatgacat ctttagacat gcaaggtttt tctataacta tcgtgcctaa taaaccagaa
     4141 tatctggaag catttttagc accaacaaca agtcaatact ttaaataagc agtgaatatg
     4201 aataaatata catttatgag gtggcacaaa tgaaagtgaa tgatatgaaa gtgcgtttat
     4261 taaatttaga agaaactttt aaaaaacatg aatctgaatt aactgaatta gatcgagcaa
     4321 ttggtgatgg tgaccacggg gttaacatgg ttcgtgggtt tagtagtctt aaagacaaac
     4381 ttgatgatag ctcaatgcaa tcattgttta aatctactgg tatggcattg atgtcaaatg
     4441 ttggcggtgc atcaggacca ctgtatggct ttagctttgt taaaatgtct gcagttacta
     4501 aagatgatat ggataatcaa gatttcatta aactaattca agcatttgcc gaagcggttg
     4561 aatcacgtgg gaaagttact ttaaatgaaa agacaatgta tgatgtagta gcacgagcag
     4621 cagagaagct taaaaatggt gaaactttaa cattcaatga tttacagcaa ttagcagata
     4681 atacaaaaga tatggtagca acgaaaggta gagctgcata ttttggagaa gaatcaaaag
     4741 gttatataga tccaggtgct caaagcatgg tttatatttt aaacgctttg attggagatg
     4801 aagataatgc ctaaaattat acttgttagc cacagtaaag aaattgcaaa tggtacaaaa
     4861 atctttgtta aagcaaatgg ctggtgacgt tgatattata ccaatcgggg gattaccaga
     4921 tggttcaatt ggaacttcat ttgatatcat ccaagaagct ttgactaaat tagatgatga
     4981 tgcattgtgt ttttacgata ttggatcttc agaaatgaat gtagatatgg caattgaaat
     5041 gtatgaaggt aatcatcgtg tgttaaaagt tgatgcacca attgttgaag gtagttttat
     5101 cgcagcagta aagctgtcaa ttggcggttc aattgatgat gcattagcag aaatcaaaca
     5161 atcattttag ttaaaattta ctaataatga aaaatgtaaa cctttttcaa atgaaacttt
     5221 ataaaaaata tgatagtata tatgtaaatg tttaataaaa tctggagaaa taggaggaca
     5281 ttgccatgca acaccttata aaaaaacatg tattgaatgg cgagtttgat ttagtacgac
     5341 aattgatgtc cgaaacagat tttatggaat ttgaagaagc atatatttca agtgcgcatg
     5401 aagtagaaag tatgatgttt tatacatgta ttttagatat gattaagtac gaagaatcat
     5461 ctgagatgca tgacttagca tttttattgc ttgtgtatcc actaagtgaa tatgaaggtg
     5521 ctttggattc tgcttattat catgcagacg cttccataaa acttactgac ggcaaagaag
     5581 ttaaaagttt gttacaaatg ttattattgc atgcgatacc aacacctgtt atttcagata
     5641 agaaggcttt tgatatcgcc aagcaaattt taaaattaga tcctaataat aatgttgctc
     5701 gtaacgtctt aaaagacact gccaaacgta tggacaacgt tgttgttgat ataaatgaat
     5761 tacatcaacg taatgcacgt taattatatt tcaattatat tagcttaata atagttttaa
     5821 catttggttg ggttgggaca caggttccag ccttttttaa tacttaaaaa ctaacgaagt
     5881 atatttgtgt gcacaaatgg tttttataca acattttata aatttataca ttttaataaa
     5941 gaacatacga tagatagttt aaaccttgtt aactgagaaa ttttgatatg tattttcgaa
     6001 acttaattaa atatacgaaa ttcaagaagc ataataatta atcatttttc ctatataaaa
     6061 gttcgtatga ctgcattata aaagcataaa tttataattt ttttaaatgt cattgaacgt
     6121 gataatgtga atagattgag caattttgaa aaagtgaaaa ataacctatg cgacttgcaa
     6181 ttaattttca gtacgttata atgcacactg tgcaaaatta aggaggtcta ttattcacat
     6241 gatgatgaat aaagaagcaa caaaaattgg atttgcctac gtcggcattg tagtgggcgc
     6301 aggattttca acaggacaag aagttatgca atttttcact aaatatggct tgtgggctta
     6361 tttaggtgtg attatatctg gttttatttt agcttttatt gggcgccaag tagcaaaaat
     6421 tggtactgcc tttgaagcga caaaccatga atcaacatta caatacgtat tcggtgaaaa
     6481 gtttagtaaa gtctttgatt atattttaat cttcttctta tttggtatag ctgtaaccat
     6541 gatagctggt gcaggcgcaa catttgaaga aagttataac atacctacat ggctaggtgc
     6601 attaattatg acattagcaa tttatgtaac attgctatta gactttaata aaatagtacg
     6661 tgcccttggt gtcgttacac catttttaat tgttttagtt gtattaatcg ctggcgttta
     6721 tttatttaaa ggtcatgttt cattagcaga agttaaccaa gtagtgcctg aagcaagtat
     6781 ctggaaggga atctggtttg gtacaatata tggtggatta gctttttctg taggttttag
     6841 taccatcgta gcaatcggtg gggatactga aaaacgtaca gtgtcaggtg caggcgcgat
     6901 gtatggtggt attatctata ctgtattact agcattgatc aactttgctt tacaaagtga
     6961 atatcctaca attaaaaatg catcaattcc aacattaaca ttagccaata atattcatcc
     7021 gttaatagca accgtgttat ctgttattat gcttgcggtt atgtataata ctattctagg
     7081 actaatgtat tcatttgcag cacgttttac agaaccatat agtaaaaatt atcatatctt
     7141 tattattata atgatggtag caggttattt attaagtttc gtaggatttg ctgaattaat
     7201 taataagtta tatacaatta tgggatatgt aggtctattt attgtagtag ctgtaattat
     7261 taaatatttc aaacgtaaaa atgcggataa aaaacatatt gcttaatatc atatgatgga
     7321 tatccgaaac tttacaattg aatcactttg ttttaatctt aaaagcaatt cgtctctact
     7381 cttatcgggc gaattgcttt ttatatttat tcagtctatt aatatgagcg tctaacaaat
     7441 agagaggtac gatgtaatga ataaagataa taaatggacg atgataactg cgctttttat
     7501 agctgtaatc agtgtattgt tagcatttca tctgaaacaa cattatgacc aaactacaaa
     7561 tgagaaccat gctaataagg acaaaattaa tattaaaaat aaaaatgtgc gcatttatca
     7621 aaaccttaca tacaatagag ttttccctaa cagtaaatta gatattatta cacctgttga
     7681 tatgtcttct aatgccaaac tgccagttat tttttggatg cacggtggtg gttatattgc
     7741 gggtgataag cagtataaaa acccattatt agcgaaaatt gctgaacaag ggtacattgt
     7801 tgtgaatgta aattatgcat tggcgccaca atataaatat cccacaccat taattcaaat
     7861 gaatcaagca actcaattca ttaaagaaaa taaaatgaat ttacctattg attttaatca
     7921 agtagttatt ggcggtgatt ctgcaggtgc tcaattagca agtcaattta ctgcaataca
     7981 gacaaacaat caattaagaa atgccatgaa atttgatcag tcattcaaac catcgcaaat
     8041 taaaggtgct atactgtttg gtggttttta taatatgcaa acagttagag aaacggagtt
     8101 tccgcgaata caattattta tgaaaagtta tacaggtgaa gaagattggg aaaagagttt
     8161 taaaaatatt tcacaaatgt cgacagtaaa acaatcgaca aaaaattatc caccaacatt
     8221 tttatctgtt ggggatagtg atccattcga aagtcaaaat atagaattca gtaagaaatt
     8281 acaagaattg aaagtaccag tagatacatt gttttacgat ggtacacatc atttacatca
     8341 tcagtatcaa tttcacctta ataaacctga atcggtagat aatatcaaaa aagtgttact
     8401 tttcttaagt cgtaatacat cttctagtgg tattcaaact gaagagaaac cacaaataga
     8461 aaatccgagt aatgaattac cgttaaatcc attaaactaa tgataaacag tagtaattta
     8521 ttattaaaac tacatttaag gatctacaaa ttgaaaacga agaatttatt acatgaaggg
     8581 ataatgtgta agagtctgag atattataaa ttgttagcgg ttctttatca tttctgctcc
     8641 actctataat gatgtgatat tgattttgct taccaatttg ttttggaact gaaaataact
     8701 ttttacaact aaatagtcga aaaacagtgt gtacatgata taataaattt ataaattgta
     8761 aagaatttaa aagaaaaatt aaaatataga ttgagcacga agtgatttga aataaggttg
     8821 tgaaagggaa tgacaaggtc agcattaaaa ccatttaaaa ataaacgcgt tatggttact
     8881 ggacgtatac aacgtgtttt gtttaaaaat tatttagata gacatagcac atttaagccg
     8941 aatgtaagga tattattaaa agatgtattt gtttcaggtg tatcaataga tcatttatgg
     9001 ttatatgaga caaataaata ctatgcattg gcaatggaac ttattcatca acgagtaaaa
     9061 tttagtgcga atgttgtacc gtattacaaa ataaatagaa ataataattt attcgtacaa
     9121 gattatggaa ttaagcgtaa aggtaggtta attactgaag aagcttacaa tcagaacaat
     9181 cagtat
//
LOCUS       DAPIDM010000106         9071 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000106 DAPIDM010000000
VERSION     DAPIDM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.106"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..568
                     /locus_tag="RJE42_002022"
     CDS             <1..568
                     /locus_tag="RJE42_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDV5877430.1"
                     /translation="ALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTKVSSETKYV
                     LGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDMLNGPVGIYH
                     NVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFRKPVNKKAE
                     TTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            588..2291
                     /locus_tag="RJE42_002023"
     CDS             588..2291
                     /locus_tag="RJE42_002023"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV5877431.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKAGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            2554..6863
                     /locus_tag="RJE42_002024"
                     /pseudo
     CDS             2554..6863
                     /locus_tag="RJE42_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
     gene            7153..7620
                     /gene="rimP"
                     /locus_tag="RJE42_002025"
     CDS             7153..7620
                     /gene="rimP"
                     /locus_tag="RJE42_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV5877432.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            7641..8816
                     /gene="nusA"
                     /locus_tag="RJE42_002026"
     CDS             7641..8816
                     /gene="nusA"
                     /locus_tag="RJE42_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV5877433.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDVDTTESTEEVTDVSVETNVEKESE"
     gene            8837..>9071
                     /locus_tag="RJE42_002027"
     CDS             8837..>9071
                     /locus_tag="RJE42_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDV5877434.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDP"
BASE COUNT         3239 a         1269 c         1843 g         2720 t
ORIGIN      
        1 ggcgttagat aaagttaaag ataataagac aactgttaaa tttgaacgtg atggtaaaac
       61 aaagtcggtt gaattaacac ctaaaaagac tgaaagaaaa ctgactaaag taagttcaga
      121 gacgaagtat gttctcggat tccaaccagc gagtgaacgt acgctcttca aaccaattgt
      181 atatggattt gaaagctttt tgaaaggtag tacactaatt tttacagctg tagtaggtat
      241 gttggctagt atatttacgg gcggattctc gtttgatatg ttaaatggtc cggttggtat
      301 ttatcataac gtagactcag ttgttaaagc gggtatcatt agcttaattg gatacactgc
      361 gttattaagt gtaaacttag gtattatgaa tttaattcct attcctgcac tagacggtgg
      421 tcgtatttta tttgttatat atgaagcgat tttcagaaaa ccagttaata aaaaagcgga
      481 aacaacgatt attgctattg gtgccatttt catggtcgtt attatgatat tagtaacgtg
      541 gaatgatatt cgacgatatt tcttataatt taggaggata aataattatg aagcaatcca
      601 aagtttttat accaacgatg cgtgacgtgc catcagaagc agaagcacaa agtcatcgtt
      661 tattattgaa agcgggtttg ataaagcaaa gtacaagtgg gatttatagt tatttaccgc
      721 tagcaacacg tgtgttaaat aatattactg caattgtgcg acaagaaatg gaacgtatcg
      781 attctgttga aattttaatg ccagcgttac aacaagctga attatgggaa gaatcaggac
      841 gttggggtgc atatggccca gaattaatgc gtttacaaga tagacatgga agacaatttg
      901 cattaggtcc aacacatgaa gaattagtta catcaatagt aagaaatgaa ttgaaatcat
      961 acaaacaatt accgatgaca ttattccaaa ttcaatctaa attccgtgat gaaaagagac
     1021 cacgttttgg tttacttcgt ggtcgtgaat ttattatgaa agatgcgtat tcattccatg
     1081 ctgacgaggc atcattagat caaacgtatc aagatatgta tcaagcgtat agccgtattt
     1141 ttgagagagt tggcattaac gcaagaccag tagttgcaga ttcaggtgct attggcggta
     1201 gccatacaca tgaatttatg gcattaagtg ctatcggtga ggatacaatt gtttacagta
     1261 aagaaagtga ctatgctgct aacatcgaaa aagcagaagt cgtttacgaa ccaaatcata
     1321 agcatactac tgtgcaacct ttagaaaaaa ttgaaacacc aaatgttaag actgcgcaag
     1381 aattggcaga cttcttaggt agaccagtag atgaaatcgt taaaacgatg attttcaaag
     1441 ttgatggcga atatattatg gttttagtgc gtggacatca tgaaattaat gacattaaat
     1501 taaaatctta tttcggcaca gataatattg aattagcaac acaagacgaa attgttaatt
     1561 tagttggtgc aaatcctggt tcactaggtc ctgtaattga taaagaaatc aaaatttatg
     1621 cagataattt tgtgcaagat ttaaataatt tagttgtcgg ggctaacgaa gatggttatc
     1681 acttaattaa tgtaaatgta ggtagagact tcaacgttga tgaatatggc gatttccgtt
     1741 ttattttaga aggcgaaaag ttaagtgatg gttcaggcgt tgcacatttt gctgaaggta
     1801 ttgaagttgg tcaagtattc aaattgggta ctaagtattc agaatcaatg aatgctacat
     1861 tcttagataa ccaaggaaaa gcccaacctt taattatggg ttgttacggt attggaattt
     1921 ctagaacgct aagtgcgatt gttgaacaaa atcacgatga taatggaatt gtttggccta
     1981 aatcagttac tccgtttgat ttacatttaa tttctattaa tcctaagaaa gatgatcaac
     2041 gagaactagc agatgcacta tatgctgaat ttaatactaa atttgatgtg ttgtacgatg
     2101 atcgtcagga acgtgcaggt gtcaaattta atgatgccga tttaattggt ttaccactgc
     2161 gaattgttgt tggtaaacgt gcatcggaag gtattgtaga agttaaagaa cgtttaacag
     2221 gtgatagcga agaagttcac attgatgact taatgactgt cattacaaat aaatatgata
     2281 acttaaaata attaagatcg aatgaattat aagagtagga aaagctgaaa gaaatcagat
     2341 gcttatgtcc tgctcttatt atttttgata taatgattat tcgatgaaaa atgactgaag
     2401 acatagtata attaaagata aatttgtttt aacaatatta tgattagaca aatataaagc
     2461 atttaatttt ctatcattac tatgcctaca taatctaaat attgttcgaa cacgtaaaag
     2521 taatttctat ttaaggtggt aattgtcttg gcaatgacag agcaacaaaa atttaaagtg
     2581 cttgctgatc aaattaaaat ttcaaatcaa ttagatgctg aaattttaaa ttcaggtgaa
     2641 ctgacacgta tagatgtttc taacaaaaac agaacatggg aatttcatat tacattacca
     2701 caattcttag ctcatgaaga ttatttatta tttataaatg caatagagca agagtttaaa
     2761 gatatcgcca acgttacatg tcgttttacg gtaacaaatg gcacgaatca agatgaacat
     2821 gcaattaaat actttgggca ctgtattgac caaacagctt tatctccaaa agttaaaggt
     2881 caattgaaac agaaaaagct tattatgtct ggaaaagtat taaaagtaat ggtatcaaat
     2941 gacattgaac gtaatcattt tgacaaggct tgcaatggaa gtcttatcaa agcgtttaga
     3001 aattgtggtt ttgatatcga taaaatcata ttcgaaacaa atgataatga tcaagaacaa
     3061 aacttagctt ctttagaagc acatattcaa gaagaagacg aacaaagtgc acgattggca
     3121 acagagaaac ttgaaaaaat gaaagctgaa aaagcgaaac aacaagataa caacgaaagt
     3181 gctgtcgata agtgtcaaat tggtaagccg attcaaattg aaaatattaa accaattgaa
     3241 tctattattg aggaagagta taaagttgca atagagggtg tcatttttga tattaactta
     3301 aaagaactta aaagtggtcg tcatattgta gaaattaaag tgactgacta tacggactct
     3361 ttagttttaa aaatgtttac tcgtaaaaac aaagatgatt tagaacattt taaagcgcta
     3421 agtgttggta aatgggttag ggctcaaggt cgtattgaag aagatacatt tattagagat
     3481 ttagttatga tgatgtctga tattgaagag attaaaaaag cgacaaaaaa agataaggct
     3541 gaagaaaagc gtgtagaatt ccacttgcat actgcaatga gtcaaatgga tggtatacca
     3601 aatattggtg cgtatgttaa acaggcagca gactggggac atccagccat tgcggttaca
     3661 gaccataatg ttgtgcaagc atttccagat gctcacgcag cagcggaaaa acatggtatt
     3721 aaaatgatat acggtatgga aggtatgtta gttgatgatg gtgttccgat tgcatacaaa
     3781 ccacaagatg tcgtattaaa agatgctact tatgttgtat tcgacgttga gacaactggt
     3841 ttatcaaatc agtatgataa aatcatcgag cttgcagctg tgaaagttca taacggcgaa
     3901 atcatcgata agtttgaaag gtttagtaat ccgcatgaac gattatcgga aacgattatc
     3961 aatttgacgc atattactga tgatatgtta gtagatgccc ctgagattga agaagtactt
     4021 acagaattta aagaatgggt tggcgatgcg atattcgtag cacataatgc ttcgtttgat
     4081 atgggcttca tcgacacggg atatgaacgt cttggatttg gaccatcaac gaatggtgtt
     4141 atcgatacat tagaattatc tcgtacgatt aatactgaat atggtaaaca tggtttaaat
     4201 ttcttagcta aaaaatacgg tgtagaatta acgcaacatc accgtgccat ttatgataca
     4261 gaggcaacag cttacatttt cataaaaatg gttcaacaaa tgaaagaatt aggcgtatta
     4321 aatcataacg aaatcaacaa aaaactcagt aatgaagatg catataaacg tgcaagacct
     4381 agtcatgtca cattaattgt acaaaatcaa caaggtctta aaaatctatt taaaattgta
     4441 agtgcatcat tggtgaagta tttctaccgt acgcctcgaa ttccacgttc attgttagat
     4501 gaatatcgtg agggattatt ggtaggtaca gcgtgtgatg aaggtgaatt atttacggca
     4561 gttatgcaga aggaccagag ccaagttgaa aaaattgcca aatattatga ttttattgaa
     4621 attcaaccac cggcacttta tcaagattta attgatagag agcttattag agatactgaa
     4681 acattacatg aaatttatca acgtttaata catgcaggtg atacagcggg tattcctgtt
     4741 attgcgacag gaaatgcaca ctatttgttt gaacatgatg gtatcgcacg taaaatctta
     4801 atagcatcac aacctggcaa tccacttaat cgctcaactt taccggaagc acattttaga
     4861 actacagatg aaatgttaaa cgagtttcat tttttaggtg aagaaaaagc gcatgaaatt
     4921 gttgtgaaaa atacaaacga attagcagat cgaattgaac gtgttgttcc tattaaagat
     4981 gaattataca caccgcgtat ggaaggtgct aacgaagaaa ttagagaact aagttatgca
     5041 aatgcgcgta aactgtatgg tgaagatctg cctcaaatcg taattgatcg attagaaaaa
     5101 gaattaaaaa gtattatcgg taatggattt gcggtaattt acttaatttc gcaacgttta
     5161 gttaaaaaat cattagatga tggatactta gttggttccc gtggttcagt aggttctagt
     5221 tttgtagcga caatgactga gattactgaa gtaaacccgt taccgccaca ctatatttgt
     5281 ccgaactgta aaacgagtga atttttcaat gatggttcag taggatcagg attcgattta
     5341 cctgataaga cgtgtgaaac ttgtggagcc ccacttatta aagaaggaca agatattccg
     5401 tttgaaacat ttttaggatt taaaggagat aaagttcctg atatcgactt aaactttagt
     5461 ggtgaatatc aaccgaatgc ccataactac acaaaagtat tatttggtga ggataaagta
     5521 ttccgtgcag gtacaattgg tactgttgct gaaaagactg cttttggtta tgttaaaggc
     5581 tatttgaatg atcaaggcat tcacaaaaga ggcgctgaaa tagatcgact cgttaaagga
     5641 tgtacaggtg ttaaacgtac aactggacag catccagggg gtattattgt agtgcctgat
     5701 tacatggata tttatgattt tacgccgata caatatcctg ccgatgatca aaattcagca
     5761 tggatgacga cacattttga tttccattct attcatgata atgtattaaa acttgatata
     5821 cttggacacg atgatccaac aatgattcgt atgcttcaag atttatcagg aattgatcca
     5881 aaaacaatac ctgtagatga taaacaagtt atgcagatat ttagtacacc tgaaagtttg
     5941 ggtgttactg aagatgaaat tttatgtaaa acaggtacat ttggggtacc agaattcggt
     6001 acaggattcg tacgtcaaat gttagaagat acaaagccaa caacattttc tgaattagtt
     6061 caaatctcag gattatctca tgggacagat gtgtggttag gtaatgctca agaattaatt
     6121 aaaacaggta tatgtgattt atcaagtgta attggttgtc gtgacgatat catggtttat
     6181 ttaatgtatg ctggtctaga accatcaatg gcttttaaaa taatggagtc agtacgtaaa
     6241 ggtaaaggct taactgaaga aatgattgaa acgatgaaag aaaatgaagt gccggattgg
     6301 tatttagatt catgtcttaa aattaagtac atgttcccta aagcccatgc agcagcatac
     6361 gttttaatgg cagtacgtat cgcatatttc aaagtacatc atccacttta ttactatgca
     6421 tcttacttta caattcgtgc gtcagatttt gatttaatca cgatgattaa agataaaaca
     6481 agcatacgaa atactgtaaa agacatgtat tctcgctata tggatctagg taaaaaagaa
     6541 aaagacgtat taactgtctt ggaaattatg aatgaaatgg cgcatcgagg ttatcgaatg
     6601 caaccgatta gtttagaaaa gagccaagcg ttcgaattta tcattgaagg cgatacactt
     6661 attccgccgt tcatatcagt gcctgggctt ggcgaaaatg ttgcgaaacg aattgttgaa
     6721 gctcgtgacg atggaccatt tttatcaaaa gaagatttaa acaaaaaagc tggattatct
     6781 cagaaaatta ttgagtatta gatgagttag gctcattacc gaatttacca gataaagctc
     6841 aactttcgat attcgatatg taaaatgaaa taatcaaggt atttatttaa ttcgtatggc
     6901 gtagttaaag aaatacaaaa ttgttgctgg acacaaagtt atacctgtat ttcttttcaa
     6961 cgtcttacga gtctattcga atgtaatgat gaaataaatg aacaaacttt tacaagaatc
     7021 tctgattatt agtgaagtca tttgtttcaa gcataaactt atgctataat taagttgctt
     7081 aaaaattagt gaactcaggc agaagagtgg gagattcccg ctcttttcta tttgccaaaa
     7141 agggaggcct gtatgagtaa aattacagaa caagtagaag tgattgttaa accaattatg
     7201 gaagacttga attttgaact tgtagacgtt gaatatgtca aagagggtag agatcatttt
     7261 cttagaatct ctattgataa agaaggtggc gtagatttaa atgattgtac gctagcttct
     7321 gaaaaaataa gtgaagctat ggatgcaaat gatcctattc ctgaaatgta ttatttagac
     7381 gtagcgtcac ctggtgcaga acgtccaatt aaaaaagaac aagatttcca aaatgcaata
     7441 actaaacctg tatttgtttc tttatatgta ccaattgaag gtgaaaagga atggttaggc
     7501 attttacaag aagtcaataa tgaaacaatt gtagtacaag ttaaaatcaa agcaagaacg
     7561 aaagatatag agataccgag agacaaaata gcaaaagcac gtcacgcagt tatgatttaa
     7621 cgtgatgagg aggaaaaaac gtgtcaagta atgaattatt attagctact gagtatttag
     7681 aaaaagaaaa gaagattcct agagcagtat taattgatgc tattgaagca gctttaatta
     7741 ctgcatacaa aaagaattat gatagtgcaa gaaatgtccg tgtggaatta aatatggatc
     7801 aaggtacttt caaagttatc gctcgtaaag atgttgttga agaagtattt gacgacagag
     7861 atgaagtgga tttaagtaca gcgcttgtta aaaaccctgc atatgaaatt ggtgatatat
     7921 acgaagaaga tgtaacacct aaagattttg gtcgtgtagg tgctcaagca gcgaaacaag
     7981 cagtaatgca acgtcttcgt gatgctgaac gtgaaatttt atttgaagaa tttatagaca
     8041 aagaagaaga catacttact ggaattattg accgtgttga ccatcgttat gtatatgtga
     8101 atttaggtcg tatcgaagct gttttatctg aagcagaaag aagtcctaac gaaaaatata
     8161 ttcctaacga acgtattaaa gtatatgtta acaaagtgga acaaacgaca aaaggtcctc
     8221 aaatctatgt ttctcgtagc catccaggtt tattaaaacg tttatttgaa caagaagttc
     8281 cagaaattta cgatggtact gtaattgtta aatcagtagc acgtgaagct ggcgatcgct
     8341 ctaaaattag tgtcttctct gaaaacaatg atatagatgc tgttggtgca tgtgttggtg
     8401 ctaaaggcgc gcgtgtagaa gctgttgttg aagagctagg tggcgaaaaa atcgacatcg
     8461 ttcaatggaa tgaagatcca aaagtatttg tgaaaaatgc tttaagccct tctcaagtgt
     8521 tagaagttat tgttgatgaa acaaatcaat ctacagtagt tgttgttcct gattatcaat
     8581 tgtctttagc gattggtaaa agaggacaaa acgcacgtct agctgctaaa ttaaccggct
     8641 ggaaaattga tattaaatca gaaacagatg cgcgtgaagc gggtatctat ccagtagttg
     8701 aagctgaaaa agtaactgaa gaagatgttg ctttagaaga tgttgacaca acagaatcaa
     8761 ccgaagaggt aactgatgtt tcagttgaaa caaatgtaga gaaagagtct gaataataag
     8821 ttggagtgaa gtatctatga aaaagaaaaa aattccgatg cgaaaatgta ttctttcaaa
     8881 tgaaatgcat cccaaaaaag atatgattcg tgttgttgtt aataaagaag gcgaaatctt
     8941 tgcggatgtt actggaaaga aacaaggccg tggcgcatat gtttctaaag atgttgctat
     9001 ggttgaaaaa gcacaacaaa aagaaatttt agaaaaatat tttaaagcat ctaaagagca
     9061 attggatcct g
//
LOCUS       DAPIDM010000107         8847 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000107 DAPIDM010000000
VERSION     DAPIDM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.107"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..136)
                     /locus_tag="RJE42_002028"
     CDS             complement(<1..136)
                     /locus_tag="RJE42_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDV5877435.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     S"
     gene            complement(159..524)
                     /locus_tag="RJE42_002029"
     CDS             complement(159..524)
                     /locus_tag="RJE42_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDV5877436.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(628..1221)
                     /locus_tag="RJE42_002030"
     CDS             complement(628..1221)
                     /locus_tag="RJE42_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDV5877437.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQINYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            1407..1754
                     /locus_tag="RJE42_002031"
     CDS             1407..1754
                     /locus_tag="RJE42_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877438.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLYENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            1771..2406
                     /locus_tag="RJE42_002032"
     CDS             1771..2406
                     /locus_tag="RJE42_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDV5877439.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            2423..3232
                     /locus_tag="RJE42_002033"
     CDS             2423..3232
                     /locus_tag="RJE42_002033"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDV5877440.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            3229..4083
                     /locus_tag="RJE42_002034"
     CDS             3229..4083
                     /locus_tag="RJE42_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDV5877441.1"
                     /translation="MKFKYHISQQETVKTFLARNDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQVHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHGKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            4104..5489
                     /gene="mgtE"
                     /locus_tag="RJE42_002035"
     CDS             4104..5489
                     /gene="mgtE"
                     /locus_tag="RJE42_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDV5877442.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            5499..7343
                     /locus_tag="RJE42_002036"
     CDS             5499..7343
                     /locus_tag="RJE42_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDV5877443.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            7621..8391
                     /gene="fabI"
                     /locus_tag="RJE42_002037"
     CDS             7621..8391
                     /gene="fabI"
                     /locus_tag="RJE42_002037"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV5877444.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAYLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(8588..>8847)
                     /locus_tag="RJE42_002038"
     CDS             complement(8588..>8847)
                     /locus_tag="RJE42_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV5877445.1"
                     /translation="FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGD
                     LGGFTLVLIAVPLYAVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT         3022 a         1239 c         1599 g         2987 t
ORIGIN      
        1 tggataagat tgctgataat ttgaagcaat cggagctaaa tggatcgaaa aatgaataaa
       61 tttcgatttt acttacaggt gatagattaa tatcttcacg actcttattt tccattattc
      121 gtaattctcc agccatgttt attcacctac aattaatttt aggaattcac catatgatta
      181 gcagttaatc ttaagcgctc aaataaataa tctccaacac cttgtggaaa cgcagcgcga
      241 ttaattgctg tctgcatatt ttctaaccat gcatctcttt caaattcagt gattgtaaaa
      301 tccatatgtc tttttcttag cataggatgt ccgtgttctt cggtataaat gtttggaccg
      361 cccaaaaact gtgttaaaaa ttgtttttgt ttacgacttg tttctgcaaa atctcctgga
      421 aacaggtgat taagtcgttc atccttttct acaagggtgt aaaaataatc aatcatatcg
      481 tataacgctt ctttaccaat gatgtcatat ggtgttgttg tcatttaatc acccattttc
      541 aaaaatttac tgttacgaac ttaagttaat atataactaa tataacatga ttttaaacat
      601 ttgaaagaaa tatgcatatt tgccaattta atttatattg tttgaaagtg tttctttttt
      661 cttaaaaaaa cgttgaactt tatttaaagg ttgatgatgt tcgaggttta gttcatttaa
      721 taaagattgg aacttttgta aaccttgatt atagtcttta acttcgaact ctaactcata
      781 atccgtagta tcgaaatact cacttttatc taaaacgagt aaatcacctt tatatttagt
      841 ttcttggcga tatgtcgtta atgcaccaag tattgataaa gttgtatcct ttacaccaaa
      901 ctgttcaact ataatttgac gaatgtcatc tggaagattg tcgtttgaaa taatcaagtt
      961 catctctggt ttaatgtcga cgatatagtt gtattctaat agaccaacct ttgctggtgt
     1021 ctttaaagtc atttcatatt gattgtcttt aactcttatg cgtagtgcag agcgatgttc
     1081 ctttaatttg aaatcgggtg tatcaatata gtaattgatt tgcttaaaaa gcacactgtc
     1141 tttaaaatat ttctcttgca atttattata gattgatgca gttatcattt gtttaaattc
     1201 tatttcatga tttgttgcca tgatatgtat acacctcgta tcaaattcaa tttatcttaa
     1261 ctatattatg aatgacaaag ttgaatttta aaagtaattt cctttatcta ttatcaatgt
     1321 taatttgacc attaaaaata gtgttcgtaa gtgttttgta ttattgaatt gtgttaaaat
     1381 gttatggaat aagaggagga ttaagcatgc gtttatatat taatgaaatt aaaattaaag
     1441 atgacatact ttattgttat acagaagatt ctattaaagg attatctgaa gtaggacaaa
     1501 tgctcgttga tagtgataat tatgcctttg cgtatacatt agatgatggt aaagcgtatg
     1561 cttatctcat tttcgtacaa gaaacatgga cgatgttgta tgaaaacatg actaaaaaaa
     1621 ttattatcaa tgatgaacta gaattgactg aattccacca agaacttact tatattttag
     1681 acaacataaa aggaaataat aattatggta aggaatttgt tgcaaccgtt gaagaaacat
     1741 tcgacattga ataaagcggg gtgaagcact atgaatcaat gggatcagtt cttaacacct
     1801 tataagcaag cggttgatga gttgaaagtg aaacttaaag gcatgcgcaa acaatatgaa
     1861 gttggtgaac aagcgtcgcc aatagaattt gttactggtc gtgttaaacc aatcgctagt
     1921 attatagata aggcaaacaa acgacaaata ccatttgata ggttaagaga agaaatgtac
     1981 gatatcgctg gtttaagaat gatgtgccaa tttgttgaag atattgatgt tgtcgtcaat
     2041 attttaagac aaagaaaaga ttttaaagta attgaagaac gagattatat tcgtaacact
     2101 aaagaaagtg gttaccgctc gtatcatgtc attattgaat atccaatcga aacattacaa
     2161 ggccaaaagt ttatattggc tgagattcag attcgtacat tagcaatgaa tttctgggca
     2221 acgattgaac atactttacg atataaatat gatggtgctt atccggatga aattcaacat
     2281 cgtttggaaa gagcggcaga agcagcgtat ttacttgatg aagagatgtc tgaaattaaa
     2341 gatgaaattc aggaagctca aaaatattac acgcaaaaac gttctaaaaa acatgaaaat
     2401 gattaacgag gtgttataaa tcatgcgtta tacaatttta actaaaggtg actccaagtc
     2461 taatgcctta aagcataaaa tgatgaacta tatgaaagat tttcgcatga ttgaggatag
     2521 tgaaaatcct gaaattgtta tttcagttgg tggtgatggt acattactac aagcattcca
     2581 tcagtatagc cacatgttat caaaagtggc atttgttgga gttcatacag gtcatttagg
     2641 attttatgcg gattggttac ctcatgaagt tgaaaaatta atcatcgaaa ttaataattc
     2701 agaatttcag gtcattgaat atccattgct tgaaattatt atgagataca acgacaacgg
     2761 ctatgaaaca aggtatttag cattaaatga agcaacgatg aaaactgaaa atggctcaac
     2821 acttgttgtg gatgttaact taagagggaa acactttgag cgatttagag gcgatggatt
     2881 atgtgtatca acaccttcgg gttcaacggc ttataacaaa gcgctaggtg gcgcactgat
     2941 acatccttca cttgaagcaa tgcaaattac agaaattgcc tcaataaata atcgtgtctt
     3001 tagaacggta ggatcaccac ttgtattacc aaagcatcat acatgtttaa tatcaccagt
     3061 taatcatgat accattagaa tgacgataga tcatgttagt atcaaacata aaaatgttaa
     3121 ttcaatacaa taccgtgtag caaatgaaaa agtgaggttt gcacgtttta gaccattccc
     3181 attctggaaa cgtgtacacg attctttcat atcaagtgat gaagaacgat gaaatttaag
     3241 tatcatatat cacaacaaga aactgttaaa acttttttag cacgaaatga tttttccaag
     3301 aagacagtga gcgccattaa aaataatggc gctttaattg ttaatgatga accagtcaca
     3361 gtgcgtaagc aattaatgcc aaatgatata ttagaaattc atttaccgcg agaaataccg
     3421 agtgttaatt taatacctta tgctcgtaag ctagaagtat tgtatgaaga tgcatttatc
     3481 atcatagtta ctaaaccaaa caatcaaaat tgtacgcctt cgagagaaca tcctcatgaa
     3541 agtttaatcg aacaggtact atatcattgt caggtacatg gtgaaaatat taacccgcat
     3601 attgttacgc gtctagatcg taatacaact ggtattgtga tattcgctaa atatggacat
     3661 atccatcatt tattttctaa agtaaacttg aaaaaaatat atacttgcct tgtatatggt
     3721 aaaacccata catctggtat tattgaagct aatattagac ggtcaaagga tagtattata
     3781 actagagaag ttgcctcgga tggtaaatac gctaaaacat cttatgaagt aataaatcaa
     3841 aatgataaat atagtttgtg caaagttcat ttgcatacgg gacgtacaca tcaaattcgt
     3901 gtacattttc aacatattgg gcatccaatt gtgggagatt ctttgtatga tggttttcat
     3961 ggtaaaattc atggtcaagt actgcaatgt acacaaatat attttgttca tccaattaat
     4021 aagaacaata tttatattac aattgattat aagcaattac ttaaattatt caatcaactc
     4081 taattcacac agggggtgta agtatgtcaa tgaacacaga tgaaaaagag cgtgttcaag
     4141 aggaattata tgatgagaca ttattagatc aatacttaga aaatgatgat attgatcaat
     4201 ttagagatga atttctagca ttacacacat atgaacaaag tgagtatttt gaagatacta
     4261 ccgatgaaaa tagacaaaag atttttcaat atttatcacc tgaagaggtt gcaaatttct
     4321 ttgatcaatt agatattgat gacgatgaat atgagttgct atttgataag atgaatgcga
     4381 catacgctag tcacatatta gaagaaatgt catacgataa tgcagtagat attttaaatg
     4441 agttgactaa accaaaagtt gctagtcttt taacattgat gaataaagat gacgcgaatg
     4501 aaatcaaagc attacttcac tatgatgagg atacggccgg cggtattatg acgacggagt
     4561 atttatcact taaagcgcat acgcctgtta aagaagcgtt attattggtc aaagcgcaag
     4621 cgccagacgc agaaacaatc tatgttatct ttgtcgttga tgatgatggt aaattagtag
     4681 gtgttttgtc gcttagagat ttaattgtag ctgaaaatga tgcttatatt gaagatatta
     4741 tgaatgaacg tgtcattagt gtgaatgtag cagacgacca agaagatgtt gctcaagtta
     4801 tgagagacta tgatttcatg gccgtacctg ttatagatta ccaagaacat ttgcttggta
     4861 tcatcacgat tgatgatatt ttagacgtta tggatgaaga ggctagtgaa gactactctc
     4921 gtttagccgg ggtttcagat atcgattcga ctaatgattc aatcattaaa acagcattaa
     4981 agcgtttacc atggttgatt attttaacat ttttaggaat gattactgcg acaattttag
     5041 ggagattcga aaaaacatta gaaaatgtag cgctactcgc agcgtttatt cctataatta
     5101 gtggtatgtc aggaaattca ggtacacaat ctttagccgt ttcagttcgt aacattacga
     5161 caggggaaat taatgagcaa agtaaattta gaattgcatt aagagaagcg ggaagtggtg
     5221 tattatcggg tgttgtatgt tcaacaatat tatttacaat tattgttgca atatatcatc
     5281 agccactttt agcattaatc gttgcaggaa gtttaacttg tgcgatgacg gtggggacgt
     5341 ttgtaggttc gatgattcca ttattgatga ataaattgaa tatcgatcca gcagtggcta
     5401 gtggaccatt tattacaaca attaatgata ttattagtat gttgatttat tttggtttag
     5461 ctacatcatt tatggcttac ttaatttaag gaggagttat ggagttttta tctttagtta
     5521 ttgttgtttt agcagcgttt ttaactccaa taattgtcaa tcgattaaac attaatttct
     5581 tgccagttgt tgttgcagaa attttgatgg ggattgtgat tggaaattca tttctaaata
     5641 tagtagaaag ggattcaatt ctaaatattt tatcaacgtt aggctttatc tttttaatgt
     5701 ttttaagtgg tttagaaatc gattttaaag cttttaaaaa agataaacgc gcacgtcaag
     5761 gacaaaatga tgatgaatcc tcaattccag gacatcttaa tctagcgtta actgtatttg
     5821 catttattat gattatttcg atccttttag cgtatgtatt taaatggctt ggattagtgg
     5881 atgatgtgtt attaatggtc attatcattt caactatttc cttaggcgta gttgttccaa
     5941 ctttaaaaga aatgaatatt atgagaacaa ctataggtca atttatccta ttagtagcag
     6001 tacttgcgga tttagtaact atgattttat taacggtcta tggtgcaatc aatggtcaag
     6061 gcggcagtac aatatggtta ataggtatat tagttgtttt cacagcaatt tcatatattt
     6121 taggtgttca atttaaaaga atgtcatttt tacaaaaatt gatggatggt acgacgcaaa
     6181 tcggtattcg tgcggtattt gcattaataa tattattagt agccctagca gagggagttg
     6241 gcgcagaaaa tatattaggt gcattcttag caggtgtcgt tgtttcatta ttaaatccag
     6301 atgaagaaat ggttgaaaag ttagactcat ttggttatgg gttctttatt cctattttct
     6361 ttataatggt tggtgtagat ttaaacatac cttcattaat taaagaaccg aaattactaa
     6421 ttatcatacc gattttaatc gttgcattta tcatttcaaa attaattcca gtcatgttta
     6481 ttcgacgttg gtttgatatg aaaacaacga ttgcatcagc atttttatta acatcaacat
     6541 tatcgctcgt gatagctgca gccaaaattt cagaaagatt aaatgctatt tcagctgaaa
     6601 cgtcaggtat attaatttta agcgcagtca ttacatgtgt attcgttccg attattttca
     6661 aaaaactgtt tccagttcca gatgagttta accgtaaaat tgaagttagt ttaattggta
     6721 aaaatcaatt aacgattcct atagcgcaaa atttaacatc tcagttatat gacgtgacat
     6781 tatattatcg caaagatttg agtgatcgtc gtcaattgtc ggatgatatc actatgatag
     6841 aaattgctga ttatgaacaa gatgttttag agcgactagg tctgtttgac cgagacatag
     6901 ttgtttgtgc cacgaatgac gatgatatta accgaaaagt tgctaaatta gccaaagcac
     6961 atcaagttga gcgtgtcatt tgcagacttg aaagcacaac ggacgataca gagttagttg
     7021 attcaggtat tgaaattttc agtagctact taagtaataa aatcttatta aaaggtttaa
     7081 ttgaaacacc taacatgttg aatttattaa gtaatgttga aacgtcacta tatgaaattc
     7141 aaatgttaaa ttataaatat gaaaatattc aattacgtaa tttcccattc ggaggagaca
     7201 tcatattcgt gcgtattatc cgtaataatg agtcgattgt tccgcatgga gatacacaat
     7261 tgcgatatgg tgatcgctta attgttaccg gtgctaaaga atacgttgat gaattgaaac
     7321 aagagttaga attttatttt taacaataat gatattaaaa tgcagacgta ttttagtacg
     7381 acgtaaaatt aatgatttta aaatgcttgt atgtatatga ttttgataaa tacatgcttt
     7441 ttacgtaaat caagtttgat acagaaagga ctaaatcaaa catttatcgt tgtaatacgt
     7501 ttaaataact ttattaaaaa gtcataatag tgttaaaata tattgacgag taaaaagtta
     7561 gttaaaactg ggattagata ttctatccgt taaattaatt attataagga gttatcttac
     7621 atgttaaatc ttgaaaacaa aacatatgtc atcatgggaa tcgctaataa gcgtagtatt
     7681 gcttttggtg tcgctaaagt tttagatcaa ttaggtgcta aattagtatt tacttaccgt
     7741 aaagaacgta gccgtaaaga gcttgaaaaa ttattagaac aattaaatca accagaagcg
     7801 tacttatatc aaattgatgt tcaaagcgat gaagaggtta ttaatggttt tgagcaaatt
     7861 ggtaaagatg ttggcaatat tgatggtgta tatcattcaa tcgcatttgc taatatggaa
     7921 gacttacgcg gacgcttttc tgaaacttca cgtgaaggtt tcttgttagc tcaagacatt
     7981 agttcttact cactaactat cgttgctcat gaagctaaaa aattaatgcc agaaggtggt
     8041 agcattgttg caacaacata tttaggtggc gaattcgcag ttcaaaacta taatgtgatg
     8101 ggtgttgcta aagcgagctt agaagcaaat gttaaatatt tagcattaga cttaggtcca
     8161 gataatattc gcgttaatgc aatttcagct ggtccaatcc gtacattaag tgcaaaaggt
     8221 gtgggtggtt tcaatacaat tcttaaagaa atcgaagagc gtgcaccttt aaaacgtaat
     8281 gttgatcaag tagaagtagg taaaactgcg gcttacttat taagtgactt atcaagtggc
     8341 gttacaggtg aaaatattca tgtagatagc ggattccacg caattaaata atatcattca
     8401 acagctttgt tcacgttatt atatatgtga gcaaagcttt ttttgctttt ataataatcg
     8461 gactgatgga aaattatttg atatttcatc tgactgattt ttttatgcac aaaaaaatcg
     8521 cttcaaaagc tgtagtttca acttttgagg cgatagtatc ttatattatt aaaagacatc
     8581 cacagaatta gtccttaaca ttactgtttg ctttatcaat aatgcgttgg cggtatttga
     8641 aaatattact aacaaccgtt ttaagtacag catataatgg cactgcaatc agaactaatg
     8701 taaagccacc taaatctcct gctgctaaaa taacaacgat aattgttaaa ggatggatac
     8761 ttaaagattt acccattaca tttggtgtaa tgatattacc ttcaagttgg tgtgcaatta
     8821 gtgtaatgat acaaacccat ataaatg
//
LOCUS       DAPIDM010000108         8841 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000108 DAPIDM010000000
VERSION     DAPIDM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.108"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..1112
                     /locus_tag="RJE42_002039"
     CDS             <1..1112
                     /locus_tag="RJE42_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV5877446.1"
                     /translation="IIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVPFTPGL
                     IPKRREEIATKIGQVIEEHLLTETLINEKLKSKQSQQAIESMIQQQLQKLTKDQLSIK
                     QITSQIDIDLEQVLQTNGNQYIASQLNNYYTKHQNQTIASLLPNQLVTFLDQHVDNAT
                     DLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQELIRL
                     TSHPKARAIVTSLITNEYQTFKDKSLNGLLDASQFNEIAENLSVYVTTYASKQANKPV
                     VTLMPQFVDYLESQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDLDYIEK
                     LIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1181..1525
                     /locus_tag="RJE42_002040"
     CDS             1181..1525
                     /locus_tag="RJE42_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDV5877447.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2063..2161
                     /locus_tag="RJE42_002041"
     CDS             2063..2161
                     /locus_tag="RJE42_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877448.1"
                     /translation="MSVGSLVVLTTTEIAFPTEIFEKNQRTQQIKF"
     gene            2458..3654
                     /locus_tag="RJE42_002042"
     CDS             2458..3654
                     /locus_tag="RJE42_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDV5877449.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFIYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            3644..6580
                     /locus_tag="RJE42_002043"
     CDS             3644..6580
                     /locus_tag="RJE42_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV5877450.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            6577..7518
                     /gene="yhaM"
                     /locus_tag="RJE42_002044"
     CDS             6577..7518
                     /gene="yhaM"
                     /locus_tag="RJE42_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDV5877451.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(7639..8601)
                     /locus_tag="RJE42_002045"
     CDS             complement(7639..8601)
                     /locus_tag="RJE42_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDV5877452.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3276 a         1254 c         1502 g         2809 t
ORIGIN      
        1 ttattatcat atttatgatt gtagtagggg cgataattgg tggtattact aatgtaattg
       61 caattagaat gctgtttcac ccctttaaac catactatat atttaaattt agagtcccat
      121 ttacaccagg tttaataccg aaaagacgcg aagaaattgc aactaaaatt ggccaagtga
      181 ttgaagagca tttgcttaca gaaactttaa ttaatgaaaa attaaaaagc aagcaatcac
      241 aacaagcaat agaatctatg attcaacagc agttacaaaa gttgacgaaa gatcaattgt
      301 caataaaaca aattacttct caaatcgata ttgatttaga acaagtatta caaactaatg
      361 gaaatcaata tattgcatca caattaaata attattatac gaagcatcaa aaccaaacaa
      421 tagcatcttt attgccaaat caacttgtaa catttttaga tcagcatgta gataacgcaa
      481 cagacttatt atgtgatcgt gcaagaaatt atttatcatc tgcaaaaggc acacaagata
      541 ttaatgatat gttggatact tttttcaatg agaaaggtaa gttaattggt atgttgcaaa
      601 tgtttatgac aaaagagagt attgcagatc gaattcaaca agaacttata cgtttaacat
      661 ctcatcctaa agcaagagca attgtgacat cgttaattac caatgaatat caaactttta
      721 aagataaatc attgaatgga ctgttagatg cgtcgcagtt taatgaaatc gctgaaaatc
      781 tatcagtgta tgtaacaaca tatgcaagca aacaagcgaa taagccagtg gttacgttaa
      841 tgcctcaatt tgttgattat ttagaaagtc aactctctag caaattagca aatttaatca
      901 ttgaacaatt atcgattcac ttatctacaa ttatgaaaaa agtggattta cgaggattaa
      961 ttgaagaaca aattaatacg tttgacttag attatattga aaaattaatt atagaaattg
     1021 ctaacaaaga actaaagttg attatgtcat taggtttcat tttaggtggt attattggat
     1081 ttttccaagg tttggttgca atctttgtct aaaaaagcga gatgtggtaa agtaatatcg
     1141 aacgttttaa taaacatcac aacaaaggag tgctataacc atggcagtaa atttatatga
     1201 ttatgcaaat caattagaac aagctttaag agaaagcgaa gaatacaaag caatcaaaga
     1261 agcattcgct aatgtaaaag ctaacgaaga atctaaaaag ttattcgacg agttccgtga
     1321 aactcaaatt aacttccaac aaaaacaaat gcaaggtgaa gaaattgctg aagaagattt
     1381 acaaaaagcg caagaacaag cgcaagcaat tgaaaaagat gaaaacatct ctgcattaat
     1441 gaatgctgaa caaaaaatga gtcaagtatt ccaagaaatc aaccaaatta tcgttaaacc
     1501 attagacgaa atttacgctg actaatatta aaataatatt tttcaatcct ttaaatagag
     1561 gaataaactg ccgacagagt ccgagacttt gtgggtagtt tttttagttt tgataacgga
     1621 agttggaggc cccctgtcaa attgggcaga tgcgttcagt atcggaagtt aggctattaa
     1681 acatgtctta actttagctc agaacggacg acggaagctg ggcaatcaaa caatattggc
     1741 tctatgccaa aacgggcaga tgagcttgat aacggaagtt agagactctc tgtcaaattg
     1801 ggcagatgcg ttcagtatcg gaagttaggc tagtaaacgt gtcctaactt ctttttgcat
     1861 tttggagtgt aaagttttca acgtaatact attagttcgg tcatgtatcg gactgatgga
     1921 aaagtgtttc acttttaatg gctcattaag aacggcttga aaatgtttgg cgtattaagt
     1981 gcaatgatag ttttgacatt tagcttctaa ttggtcatta ctgccgagca aatctagtag
     2041 agtaattatg taaatattta atgtgtcagt tggttcacta gtggtgttaa caactacgga
     2101 aattgcattt ccgactgaaa tttttgaaaa aaatcaacgt acgcaacaaa taaaatttta
     2161 aactgttata aatgtgtctc aatttcatat gttcatcgac gatatgaagc gtattatggt
     2221 aaaatgaaga aataataaac ttgttaataa ataaaacatc acgatttgac taaagcactt
     2281 tattattgtg tagataataa ttttttaacg aaattaaaat aaaaatggcg actggtttta
     2341 ataaatcagc taatgaatca ctacacctat aagtatgaat atagtgatta gaatgctttg
     2401 tatagttgga ttttgcaaaa ttgatgttag aacctttaat taaggagata tggatgtatg
     2461 gttaaattta ttcattgttc ggatttacat ttagatagtc ctttcaaatc taagagtcat
     2521 ataagcccta aaatttttga ggatgttcaa aaaagtgctt atgaaagctt taaaaacatt
     2581 gtagatattg cattacaaca agatgttgat tttgtaatta tcgcgggtga tttgtttgat
     2641 agtgaaaaca gaacattacg tgctgaaatt ttcttaaaac agcaatttga acgtttacaa
     2701 aatgaacaaa tctttattta tgtttgtcat ggtaatcacg acccattatc atcaaagatt
     2761 tcatcaaact ggccagataa tgtttctgta ttttcaaata aagttgagac gtatgaagca
     2821 attactaaat ctggtgaaac aatttatatt cacggattta gttatgaaaa tagagcaagt
     2881 tatgagaaca agattgatga atatccgtca agtcaaggcc aaaaaggcat acatattggt
     2941 gtcttgcatg gtacgtacag taaatcttct gttaacgaaa gatataccga gttcatttta
     3001 gaagatttaa acagtaaatt gtatcattat tgggctttag gtcatataca tgaacgtcaa
     3061 caattaagtg atatgcctgt aattaactat tcaggtaata ttcaaggtag acattttaat
     3121 gagcaaggtg aaaaaggttg cttattaatc gagggtgacc acttaaaatt aaaaactaaa
     3181 ttttatccta cacagtatat tagatttgaa gaagcaacta ttgaaacgga taagacatct
     3241 aagcaaggtt tatacgaggt cattcaaaac tttaaagaac aagtgagaga agaaggaaaa
     3301 gccttttatc gtttaacgct tgttattaat agtgagacat taatttcacc tcaagattta
     3361 ttacaagttg aagaaatgat tacagattat gaagaaaacg aaaatcaatt tgtatatatt
     3421 gatgagttaa aaatacaata tgcacaaaat gatgagtcac ctttagttaa tgaattttca
     3481 gcggaattat tagtcgatca aactgttttt gataaagcga tgtcagactt atatttaaat
     3541 ccaagggcat ctaagttcct agacgattat ggaacattcg accatacagc attagttaat
     3601 cgtgctgaag aaatattaaa agcagaaatg agaggtgaac aaaatgataa ttaaatcact
     3661 tgaaatttat ggttacggtc aatttgttca acgtaaaatt gaatttaata aaaacttcac
     3721 tgaaattttt ggtgaaaatg aagcgggtaa atcgacgatt caagcattca tccattcgat
     3781 attatttggt tttccaacta aaaagtctaa agagccaagg ctagaaccac gtctaggtaa
     3841 ccaatatggt ggtaaattag tacttattct tgatgatggc ttagagattg aagttgaacg
     3901 aattaaaggc agtgctcaag gtgatgtgaa agtatattta cctaatggtg ctgtgcgtga
     3961 tgatgcttgg ttacaaaaga aacttaatta tatttctaaa aagacatatc aaggtatctt
     4021 ctcatttgat gtactagggc ttcaagacat tcatagaaat ctaaatgaaa aacaattgca
     4081 agattattta ttacaagcag gggctttagg atcaactgaa ttcacgtcaa tgcgcgaagt
     4141 gattaatcgt aaaaaagatg aattatataa aaaatcaggt aaaaatccaa tcattaatca
     4201 acaaattgag caattaaaac aactagaaag tcaaattcgt gaagaagaag ctaaactaga
     4261 aacatatcat cgcttagtag atgatcgaga taaatcatca cgtcgattag agaatttaaa
     4321 gcataattta aatcaattat caaaaatgca tgaagaaaaa caaaaagagg ttgctttaca
     4381 tgatcattca caagaatgga agtctctaga acaacagtta aatattgagc caatcacatt
     4441 cccagaaaaa ggtgtagatc gttacgaaaa agcacgagcg cataagcaat cgttagaaag
     4501 agatattggt ttaagaaatg agcgtttagc tcaacttaaa gaagaagcaa ctcaattaga
     4561 gccagttaaa caatctgata ttgacgcctt cattagtttg aatcaacaag aaaatgaaat
     4621 taaaaataaa gaatttgaac ttactgcaat cgaaaaggat attgcgaata aacaacgtga
     4681 taaagatgaa ttgcaagcaa atattggttg gtctgaaacg catcatgacg tagatagttc
     4741 tgaggcaatg aaaagttatg tcagtgagca aatcaagaat aaacaagaac aagctgcata
     4801 cattaaacaa ttagaacgta gtttagaaga aaataaaatc gaagataatg cggttcatag
     4861 cgaactagat tctgttgaag aaaaaatagt tcctgaagag acttttgaaa agaaaaaaga
     4921 atactcacaa caagtcattg aattaaacga aaaagagaac ttgtatagca agttgaaaga
     4981 acgtttcgaa attgagcaac aagaaaaaca aaaacgtcaa aaattgttac gaacaacgtt
     5041 tattctttta acattagtag gtattggttt aactgccttc tcatttattt caaacaacat
     5101 gttgtttggt attattttcg cagtattaac tttagtattt gttatcggta tcatcatgtc
     5161 taaatcaaaa gaagtagatt atagcgaggc catcacagat gaaatcgaag aaattaaagc
     5221 acaactagcc attttagatg aaaactatga cttagatttt gatcttgatg agcaatatcg
     5281 tattcgagat cactggcaac aagctttgaa aaataaagat attttagaag agaaacgtca
     5341 gtatattgaa ggtcgtttaa atgatgcgaa agggcgtcat gatgaattac aatcgacagt
     5401 tgaaaacgtt aaggatgaat tatacttatc atctaaaatt tcaaatgatt taattgttga
     5461 tagtatttct acaatggcga atattaaagc attagatcaa catatcagtg atttaaatca
     5521 acaacgccag caattggttc aagaattaga tacattctac aatcatgctg aagctgtaac
     5581 aaaatcacaa tttgtttatt tcaacaaatt atctttattc cacgatgtgc aacaatggtt
     5641 gaaatcagct gaagacacga atgaaaaatg gcgtattaat gctgaaaata ccaaacttgt
     5701 tacaaatgaa ttaaatcatt taaatgctca attagaagaa aataataaag aaattacagc
     5761 actattcgat tttatcaatg ttggtactga agaagatttt taccaacatc atgaagatta
     5821 ccaaacatac actagtaatt tgagtcgttt taatgattta actaaatatc ttgagaacca
     5881 aaactattct tatgaattaa gttctagttt aagtgaaaaa acaacagcac aacttgaaga
     5941 agaagatcat ttattggcta ctcaagttga cgaatataat gagcaatatc ttgaaatgca
     6001 agcacaagtc agtgatttaa gtgcacaaat caatcacatg gaaactgata caacgcttgc
     6061 taatttaaga catgaatatc acagtcttaa aaatcaactt aatgatatcg caaaagattg
     6121 ggcaagttta agttatttac aaagtttagt tgatgaacac attaaacaaa ttaaagataa
     6181 acgtttgcct caagttatta atgaagcggt agaaatattg aagcatttaa cagatggcag
     6241 atatacgatg attaactata atgaagattc aattacggtt aaacatgtta atggtcaatt
     6301 atatgatcct gttgaactaa gtcaatctac aaaagaatta ctttatgtag ctttacgtat
     6361 cagtttaatt aaggtactac gaccatatta tccgttccca ttaattgttg atgatgcatt
     6421 tgttcatttt gataaaaaac gtactgaaaa aatgttgaat tatttacgat cattatcaga
     6481 acactatcaa gtactatact ttacatgtgt aaaagataat attgttccat caaaagaagt
     6541 gattacatta aacaaaatag aggaaggcgg gaaacgatga gaaatataga gaatctaaat
     6601 cccggagatt cagttgatca ctttttctta gtgcataaag ctacacaggg tgtaacagca
     6661 caaggtaaag attatatgac attacatttg caagataaaa gtggtgaaat tgaagcgaaa
     6721 ttttggacgg ctacaaaaaa tgatatggca acaatcaagc ctgaagaaat tgtacatgtt
     6781 aaaggtgaca tcataaacta tcgcggaaat aaacagatga aagtcaacca aattagacta
     6841 gcgacaactg aagatcaatt aaaaacagaa caatttgtag atggtgcacc tttatcaccg
     6901 gcagaaatac aagaagagat ttctcattat ttgctagata ttgaaaatgc taatttacaa
     6961 cgtatcacac gtcatttatt gaaaaaatat caagaacgat tttacacata tccagctgct
     7021 agttctcatc atcataactt tgcgagtggc ttaagttatc atgtattaac gatgttacgt
     7081 attgcaaaat caatttgtga catttatcca ttgttaaaca aaagtttgtt atatagtggc
     7141 attattttgc atgatattgg taaagttaga gaattgagtg gtcctgttgc gacgtcgtat
     7201 acagtcgaag gtaacttatt aggacacatc tcgattgcga gtgatgaagt agttgaagca
     7261 gctcgtgaat tgaatattga aggagaagaa atcatgttgt taagacatat gattttatct
     7321 catcatggta agttagagta tggttcgcca aaactgccat acttaaaaga agcagaaatt
     7381 ttatgctata tcgataatat cgatgctaga atgaatatgt ttgaaaaggc ttataaaaaa
     7441 actgacaagg gtcagtttac agataaaata tttggtcttg aaaatcgtag attctacaat
     7501 cctgaatcac tcgattaaga attaaaagat atcggacaga tgagtatact gaaacatgag
     7561 ctcatctgtc cgatttttaa tgcgaaaaaa ccgagcggcc gtggtatgat ttttaaacca
     7621 cggtcgctcg gttttgtgtt attggctcat gccggattgt ccgccttgtg cgccaccttg
     7681 tttaagtttt tcagggttta agattttatc ttcgacaact gatttaatat cacgatcttt
     7741 aaagtcaaca tcgtattctt ttaatagatc tttgtatgca tcagttaata attttggatt
     7801 tttttgtact ttctgatcga ctaatttttc tttcaggctt tgtttttcac tgttaaagtc
     7861 tgttggttta tcagctttaa taatatgata tccaaaactt gatttaacaa cgtctgatac
     7921 ttcaccatct ttaagcttaa atagtgcttt ttcaaaatct ttatcagttt gtcctttaag
     7981 aacataacct aattcgccat cttttttagc tgaaccagta tccattgatt cttttttagc
     8041 gatttcacca aatttacttg gatcttttga aacttctttt tgaatttctt cagctttttg
     8101 tttcgcttct ttatcatcta agccttcttt gtcgcttttc ttagatttaa ctttaattaa
     8161 aatgtgtgaa gctttcttgc tgtcttcttt aatttcagaa tcagagattt taattttatc
     8221 tgatagtaat tctttatgat aagcagcagt acgtaaattt tctttatatt tatcggctgt
     8281 taaaccttgc tgttgaaggg ctttttcaaa tttatcttta ccgccgtatt gcttttgcat
     8341 tttttcaatt tgttcgtcaa tcttcttatc attaacttta tttttatatt tatcagctaa
     8401 aattttattt aacatttcag taaatgatgc atttgcaatt tgatctttac cgattttttt
     8461 cattgtatct gcaactgtta catctccagc tttagaagaa attaatgtat tttctttaga
     8521 gtctgtggca ctagcgccac aagcgcctaa taataaagca ctagctgtta ccggaacgat
     8581 taatttgttt atcatcttca tagttgaaac tcctttgtaa gttaatcatc gtgaatataa
     8641 catagtttta tattatcacc aatcaataac ccatgacatc agactagagc attaggttta
     8701 ggggtagaaa ttgatgaaaa taaaaaaaca aggtctatat acatttgatt gtaagctatt
     8761 acatagccta aaaacaaata ataacacctt gctataatta ttttgttata aatgatatgg
     8821 atttttaatt tttttcaaaa t
//
LOCUS       DAPIDM010000109         8763 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000109 DAPIDM010000000
VERSION     DAPIDM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.109"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..2054
                     /locus_tag="RJE42_002046"
     CDS             <1..2054
                     /locus_tag="RJE42_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDV5877453.1"
                     /translation="SNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQIAQGSHYK
                     QIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEMLDTAEKLS
                     KLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDKQLLSKIGK
                     SQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSKLPGVNTSM
                     DWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYLEYQYEDVL
                     RGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDKQIKKLRSQ
                     GAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGSSVKGGTLL
                     AGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSNVYMFKTAL
                     KLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETKGQIEPLTNNPGN
                     YLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDDVGPLKKKINGT
                     VLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEVFQNGEPRV
                     NSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGKDNKNKDKD
                     K"
     gene            2167..2316
                     /gene="rpmG"
                     /locus_tag="RJE42_002047"
     CDS             2167..2316
                     /gene="rpmG"
                     /locus_tag="RJE42_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV5877454.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            2525..3064
                     /locus_tag="RJE42_002048"
     CDS             2525..3064
                     /locus_tag="RJE42_002048"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV5877455.1"
                     /translation="MTKNEIRKYILHKMKTFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDKKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDNGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3076..4539
                     /locus_tag="RJE42_002049"
     CDS             3076..4539
                     /locus_tag="RJE42_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV5877456.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQIFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNVFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            4520..4723
                     /locus_tag="RJE42_002050"
     CDS             4520..4723
                     /locus_tag="RJE42_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF910 family protein"
                     /protein_id="HDV5877457.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPDDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            4720..5706
                     /locus_tag="RJE42_002051"
     CDS             4720..5706
                     /locus_tag="RJE42_002051"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDV5877458.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFTPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            5706..6035
                     /locus_tag="RJE42_002052"
     CDS             5706..6035
                     /locus_tag="RJE42_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDV5877459.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            6032..6655
                     /locus_tag="RJE42_002053"
     CDS             6032..6655
                     /locus_tag="RJE42_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV5877460.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            6707..7681
                     /locus_tag="RJE42_002054"
     CDS             6707..7681
                     /locus_tag="RJE42_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDV5877461.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVTQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            7653..8723
                     /locus_tag="RJE42_002055"
     CDS             7653..8723
                     /locus_tag="RJE42_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDV5877462.1"
                     /translation="MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
BASE COUNT         3287 a         1146 c         1553 g         2777 t
ORIGIN      
        1 aatcaaatga tgaaatcgtt caaaatacaa ttaacaagag aattaacttt atatttggtg
       61 tgattgtatt tatttttgca gtactagtac tacgtttagg ttatttacaa atcgcacaag
      121 gctcacatta taaacaaatt ataaaaaatg atgaaaacat tacagtgaat gagtctgtgc
      181 caagaggtcg tattttagac agaaatggga aagttttagt tgataatgct tctaaaatgg
      241 ctattacata tactaggggt cgaaaaacaa cacaatcgga aatgttggat acggctgaaa
      301 agttatcaaa gctaatcaag atggatacta agaagattac agaacgtgat aaaaaagatt
      361 tctggattca gttgcatcct aaaaaagcaa aagcaatgat gacaaaagaa caagctatgt
      421 tagcagatgg aagtattaaa caagatcaat atgataaaca actgttatcg aaaatcggaa
      481 aatcacaatt agatgaattg tcttctaaag atttacaagt tttagccatt tttcgagaga
      541 tgaatgcagg aacagtttta gatccacaaa tgataaaaaa tgaagatgtc agtgaaaaag
      601 agtatgcagc agtttctcag caactttcca aattaccagg tgtaaataca tcgatggact
      661 gggatagaaa atatccttat ggcgatacat taagaggtat attcggagat gtatcgacac
      721 ctgctgaagg tattccaaaa gaattgacag aacattactt atccaaagga tattcacgca
      781 atgatcgtgt tggaaaatct tacctagaat atcaatatga agatgtattg cgtggtaaga
      841 agaaagaaat gaaatacaca acggacaaat ctggtaaagt tacgtcttca gaagtgttaa
      901 atcctggcgc tcgcggccaa gatttgaaat taacgatcga tatagatctt caaaaagaag
      961 tagaagcatt attagataaa caaattaaga agcttcgcag ccaaggtgca aaagatatgg
     1021 ataatgctat gatggtagta caaaatccta aaaatggaga cattcttgcg cttgccggaa
     1081 agcagattaa taagagtggt aaaatgactg attatgacat tggtacgttt acttctcaat
     1141 ttgcagttgg atcttctgta aaaggtggaa cattattagc tggttatcag aataaagcta
     1201 tcaaagttgg agaaacaatg gtcgatgaac cattacattt ccaaggtggt ttgacaaaac
     1261 gatcatactt taataaaaac gggcatgtat ctattaatga taagcaagct ttgatgcatt
     1321 catcaaacgt atatatgttt aaaacagcat taaaattagc gggagaccct tattattctg
     1381 gtatggcttt accatcagat ataagttcac ctgcccaaaa gctaagaaga ggattgaatc
     1441 aagtaggctt aggtgtgaaa acaggtatag atttaccaaa tgaaacaaaa ggtcaaatcg
     1501 aaccattaac aaataatcca ggtaattatc tagatttatc aattggtcaa tatgatacct
     1561 atacaccatt acaattatca caatatgttt caactatagc gaatgatggt tatagaatac
     1621 aaccacacat tggattaacg attcatgaat caactaataa agatgatgtt ggtccactca
     1681 agaagaaaat taatggcact gtcttgaaca aggttaataa tactgaaaaa gaaatcaaac
     1741 aaattcaaga aggattcaaa atggcattta atgataaaga tggtactgga tatgttagtt
     1801 ttaaagatac agtagtacct actgctggta aaactggtac cgctgaagtg ttccaaaacg
     1861 gagagccaag agttaactct acttatatag gatacgcgcc aattgatgat ccaaaattag
     1921 cgttttcaat tgtatataca aatcagcctg taccaccacc atggttaaca ggtggagact
     1981 taggtagaga tgtaattaac tactacttta agcagttagg taaagataat aaaaataaag
     2041 acaaagacaa ataaaattta acctgacgat tgtgtagcgc atggttgtaa aattttaact
     2101 ttgcaaatat tatagatgtt ggtataataa taaagtcgta tttagaaatg ataaggagga
     2161 ataaccatgc gcgtaaacgt aactttagct tgtacggaat gtggtgacag aaactacatt
     2221 acaactaaga acaaaagaaa taatccagaa cgtgttgaaa tgaagaaatt ctgttcacgt
     2281 gaaaacaaac aaactttaca ccgtgaaaca aaataatcat taaaattaaa tttaaattaa
     2341 caataaaaaa ggtgaaatct caaaataaga ggtttcacct ttttaattgt gcacacgtat
     2401 gaaatgataa aagattttca attgaaataa taaattttag aattgtcgca taattcatca
     2461 tgacaacata atgacatgta ttgttattaa cgatataata gaattgaata aagtgggtga
     2521 taatgtgact aaaaatgaga ttagaaaata cattttacat aaaatgaaga cttttaataa
     2581 agctgaaaag cgaaaagcag acacatggtt aagaaatcaa ttttttgcaa ctgaagaata
     2641 caaagaagca aacgcaattg cgctagttct ttcttttaat catgaagtag atactttttc
     2701 tattattgaa caagccttaa tggatcataa acgtattttt gtgccgaaaa tggattattt
     2761 aaatcatcaa atgactttta aagagatatt taatctaaaa gatattgatg tagataagaa
     2821 ggggatttac tatccaactt caaaaggtga aacaacgaat aacctagatt taattgttgt
     2881 tcctggtgtt ggatttcaag acaatggata tagaattggg tatggtggtg gctattatga
     2941 caggttttta gctaattatc agacaaagac aataagctta ttatacgatt ttcaaataac
     3001 atcatttgaa ccggaatcat tcgatcaacc agtcgataaa ttgattatat atcaatcagc
     3061 atagtggagg actaaatgaa catagacaaa caattttgga aaacaatata ttattggatt
     3121 aggtacttga attttgatat tgtcagtagg gaaaaagatg accaagaaat ttggttagcc
     3181 cataaaagaa aaaaacaagt agtcattttt aaacaacata tcaagtctac ccaagaaata
     3241 cgttttgaca aagcgaaagt gcttgaacac aaagatgaaa tagcaaattt tatttctttc
     3301 gaaccacaaa tttttgaatt ttattatttt acagaatcgg aattttcaga agaacaatta
     3361 aatgaagttt cgccaattag aattaaattc aatgttataa gacacacaaa agatttgata
     3421 aaacatatgc cgaatatatt tttggctaga cttatttcag aagataatga taaaaagaca
     3481 tatatgttct ataaacgcaa agtattaacc gataactttt tagataaata tatgcagaaa
     3541 ttttcaccgg caacatacac aataatattt gtaaatgtct taatatggtt atgtatgatt
     3601 ttatatttaa ataatttttc ggatgtaaaa ttattagatg ttggcgggtt agtgcatttt
     3661 aatgtcgtgc atggtgaatg gtatcgaatt gttacatcga tgtttttaca ttttagtttt
     3721 gaacatatac ttatgaatat gctttcattg tttatttttg gtaaaatagt cgaagcaatt
     3781 attggttcat ggaggatgtt aactgtatac tttattgcag ggctgtttgg aaactttgta
     3841 tcactatcat ttaacacgac tacaatttca gttggggcta gtggtgctat attcggtctg
     3901 atcggatcaa tttttgcgat gatgtatgtt tcaaaaacat ttaacaaaaa aatgttagga
     3961 cagttattaa ttgcattagt gatattagtt ggtgtttctc tgtttatgtc aaatataaat
     4021 attgtggcgc atattggagg cttcattggt ggtttattaa taactttaat tggctattac
     4081 tataaagtga atcgtaatgt tttttggatt ttactaatcg gtatgcttgt tatatttatt
     4141 gcacttcaaa ttagaatttt tacaattaaa gaagataata tttataataa attgatcaaa
     4201 gatgatatga ctagtggtaa ttatgataat gctcaaaata ttgtgaagca gacaataaat
     4261 aaaaattatg ccgatgatca aacatattat ttaagtggta tgattatggc tactatcaat
     4321 tctaagtctg aaggtatgac agaatgggag cgaggactta gaatgtttcc taaatcaggt
     4381 ttattaaatt ttgagttagc gatagcaaat cgttcattaa atgatgatga aaaagcatta
     4441 aaatatgtgc gtaaagcatt aaatgcagat cctaaaaata cagattatat taacttagaa
     4501 aaagagttga ctaaatcaaa tgagtcgaaa aataaataac ttttatgatg tacaacagtt
     4561 attgaaaagt tacggatttc taatatattt taaaaatcca gatgatatgt acgaaatgat
     4621 tcaacaggag atttcatcat tgtatcaata tgaactgtta tctaaggaag aatatttgaa
     4681 atgtacgttg ataattaatc agagaaggaa tgaacagaaa tgacaaaaat tattttagca
     4741 gctgatgtag gcggaacgac ttgtaaatta ggtattttca cacctgaatt agaacaatta
     4801 cataaatggt ctattcacac tgatacatct gatagtacag gatatacact tttgaaagga
     4861 atttatgatt cgtttgttga aaaagtaaat gaaaataatt ataatttttc aaatgtactt
     4921 ggcgtaggta ttggtgtacc aggtcctgtt gactttgaaa aaggtacagt aaatggagca
     4981 gtaaacttat attggccaga aaaagttaat gtacgtgaga tttttgaaca attcgttgat
     5041 tgtccagtgt atgtagataa tgatgctaac atagctgctt taggggagaa acacaaaggt
     5101 gctggtgaag gtgccgatga tgttgttgcc atcacacttg gtacaggact aggtggagga
     5161 attatttcaa atggtgaaat cgtacatggt cataatggct ctggcgcaga aataggtcat
     5221 tttagagcag acttcgatca acgatttaaa tgtaattgtg gtcgttctgg atgtattgaa
     5281 acagttgctt cagcgacagg cgttgttaac ttagtcaact tctactatcc gaagttgacg
     5341 tttagatctt ctatattaga attgattaaa gaaaataagg ttacagcaaa agctgttttt
     5401 gatgcagcaa aagctggtga ccaattctgt attttcatta ctgaaaaggt tgcaaactat
     5461 attggatatt tatgtagtat tattagtgtt acaagtaatc cgaaatatat cgttctaggt
     5521 ggaggaatgt ctactgcagg acctatttta attgaaaata ttaaaacaga atatcataat
     5581 ttaacattta cacctgctca atttgaaact gaaattgtac aagcgaaatt aggtaatgat
     5641 gcaggtatta caggagcagc aggattaatc aagacctatg tattagataa agagggggta
     5701 aaataatggc tattgttgat gtggttgtta ttccagttgg aacggaaggt ccgagtgtta
     5761 gtaaatatat tgcagatatt cagaaaaaac ttcaagaata taaagcgatg ggtaaaattg
     5821 attttcaatt aacaccaatg aatactctaa ttgaaggtga attaagcgat gtattagaag
     5881 ttgtgcaagt gatacatgaa ttaccttttg ataaaggttt aagtagagtt tgtacaaata
     5941 tccgtattga tgaccgacga gacaaatcta gaaaaatgaa tgataaacta acatcagtac
     6001 aaaaacattt agaaaatagt ggtgaaaacc tatgaggatt tcaagcttaa ctttaggctt
     6061 agttgatact aatacgtatt tcatcgaaaa tgacaaagct gttattctga ttgacccttc
     6121 aggtgaaagt gaaaaaatta ttaaaaaatt aaaccaaata aataaaccgt taaaagctat
     6181 tttattaaca catgcacatt ttgatcatat cggagcagtc gatgatatag ttgatcgatt
     6241 cgacgttccg gtttatatgc atgaagcaga gtttgatttt ctaaaagatc ccgttaaaaa
     6301 tggggcagat aaatttaagc aatatggatt accaattatt acaagtaagg taactcctga
     6361 aaagttaaac gaaggtagca cagaaataga aggatttaag tttaatgtgt tacacacacc
     6421 tggacattca ccaggaagtt taacatatgt gttcgatgaa ttcgcagttg ttggagatac
     6481 attatttaat aatggaatcg gacgtacaga tttatataaa ggtgattatg aaacgctcgt
     6541 tgattctatt caagataaaa tatttgaatt agaaggcgat ttacctttat tccctggaca
     6601 tggtccatat acgacggttg atgatgaaca attaaatcca tttttacacg gttaaattga
     6661 agtaataaaa acacctccta cgtgattata tgtaggaggt gtttttttga agattctatt
     6721 tcaagaaata attaataaag cgatagaaat gaaagcgagt gatgtacatt ttattccagt
     6781 taaaaatgaa gtaagtatta aatttagaat taatgataac ttggagcagt acgaacaaat
     6841 tgggaatagc atttatcaaa agttattagt ttatatgaag tttcaagctg ggcttgatgt
     6901 ttctacacag caagtcacac agagcggtcg atatagttac catttcaata aaatatattt
     6961 tttgagaata tcaactttac cattgtcact tggccaagaa agttgtgtta tcagaattgt
     7021 acctcaattt tttcaacaac agaaatcaac ttataaattc aatgatttta aacacctcat
     7081 gaataagaaa caaggattac tattgtttag tgggccaact ggttcaggaa agagtacatt
     7141 aatgtatcaa atggtctcat acgcgaataa agccttgaat ttaaatgtaa tttctataga
     7201 ggatcctgta gagatgcaaa ttcctggtat tgtccaaatt aatgtgaatg ataaagctgg
     7261 cattaactat gtaaattcgt ttaaagctat tttaagatgt gatcctgatg ttattttaat
     7321 aggtgaaatc agagataaag atgttgccaa gtgtgttata caggctagtt taagtggtca
     7381 ccttgttctg actacattgc atgcaactga ttgtaaaggt gctattttaa ggctattaga
     7441 aatgggcatt tctgtacaag aattgataca ggcaactaac ttaattataa accaacgact
     7501 tgtaactact attaagcaac agcgacaatt agtatgtgaa attctatctc agcaacaact
     7561 ccgatatttc ttttcccata atcattcatt accatcatca tttaagaact tagaagataa
     7621 acttgatgat atgacaaaag caggtgtcat ttgtgaaact acaatggata aatacattta
     7681 aactacattc taagaagcga caattaagta aggtccaaca aatcgattta ctttcaaatt
     7741 tatgtaattt gttgaaatat ggtttcactc tgtatcaaag ttttcaattt ttaaatcttc
     7801 aaatgacata taaaaataag caattaggca ccaccattct aagtgaaatt tcaaatggtg
     7861 caccatgcaa ccagatatta tcactgatag gttatagcga tactatcgtc atgcaagtat
     7921 atttggcaga aagatttggc aatatcatag acgttctaga agaaaccgta aattatatga
     7981 aagtgaatag aaagtcagaa caacgattgt taaagacact gcaatacccc ttaatactag
     8041 tctctatttt tattgctatg attattatat taaacctcac agtaattcca cagtttcaac
     8101 aattatatac ttctatgaat attcaactat cttcttttca aaaaacattg tcttttttca
     8161 ttaccagctt acctactata attgtagtaa tgctcataat agtatctatg ttggctatta
     8221 ttatgaaatt aatttataac aaattaaata tgctcaataa gataaacttt gtgatgaaac
     8281 taccgctaat atcgggttat ttccaattat ttaaaactta ttttgtaact aatgaattag
     8341 tgttgtttta taaaaatggt attacacttc aatcaatagt agacgtttat attaaccata
     8401 gtagtgatcc atttagacag tttctaggta aatacttatt aacttattca gaaatgggat
     8461 atggtttacc tcaaatttta gaaaaactaa aatgctttaa gcctcaatta attaagtttg
     8521 tgctacaagg tgaaaagaga ggaaagctag aagtagaact aaagttatat tcgcaaatat
     8581 tagtaaaaca aatagaagat aaagcgataa aacagactca gtttttacag cctattttat
     8641 ttttgatttt aggtttattt attgtcgcaa tttatttagt aattatgtta ccaatgtttc
     8701 aaatgatgca aagtataaaa taatggaggt taatatatgt ttaaatttct aaaaaaaact
     8761 caa
//
LOCUS       DAPIDM010000110         8723 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000110 DAPIDM010000000
VERSION     DAPIDM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.110"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            28..321
                     /locus_tag="RJE42_002056"
     CDS             28..321
                     /locus_tag="RJE42_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDV5877463.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            455..1999
                     /gene="miaB"
                     /locus_tag="RJE42_002057"
     CDS             455..1999
                     /gene="miaB"
                     /locus_tag="RJE42_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV5877464.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKQVE
                     PEMVIQ"
     gene            2000..2365
                     /locus_tag="RJE42_002058"
     CDS             2000..2365
                     /locus_tag="RJE42_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDV5877465.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQASQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            2392..2883
                     /gene="thiW"
                     /locus_tag="RJE42_002059"
     CDS             2392..2883
                     /gene="thiW"
                     /locus_tag="RJE42_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDV5877466.1"
                     /translation="MKSRKLAITALLISINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            3186..5804
                     /gene="mutS"
                     /locus_tag="RJE42_002060"
     CDS             3186..5804
                     /gene="mutS"
                     /locus_tag="RJE42_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV5877467.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDHLKRQINMVTETITVRETLSTEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQDTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNMQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENSNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            5817..7826
                     /gene="mutL"
                     /locus_tag="RJE42_002061"
     CDS             5817..7826
                     /gene="mutL"
                     /locus_tag="RJE42_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV5877468.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMAENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            7832..8374
                     /locus_tag="RJE42_002062"
     CDS             7832..8374
                     /locus_tag="RJE42_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV5877469.1"
                     /translation="MNQLNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         3408 a         1119 c         1591 g         2605 t
ORIGIN      
        1 ttacaaaaaa taaaggagtt tttaaaaatg aaagacacat taatgagtat acaaataatt
       61 cctaaaacac caaacaatga caatgttata ccttacgtag acgaggcgat taaaataatt
      121 gacgaatctg gtttacattt tagagtaggt ccgttagaaa cgacagtaca aggaaatatg
      181 aatgaatgtt taattttaat acaatcatta aatgaacgaa tggtggaact tgaatgtcca
      241 agtattatta gccaagttaa gttttatcat gtgccagacg gcatcactat tgaaacttta
      301 actgaaaaat atgatgaata atattaaaag tgaagtaaac tggatttgaa ttggcttgtt
      361 agagatgacg tataacttta actgtttttg cactttatag ttaaatttaa tataattatt
      421 aaatgatacg ggcaaataga aaggattttg taaagtgaac gaagaacaaa gaaaagcaag
      481 ttctgtagat gttttagctg agagagataa gaaagcagaa aaagattata gtaaatattt
      541 tgaacatgtt tatcaaccgc ctaatttaaa agaagcgaaa aaaagaggta aacaagaagt
      601 tcgttataat agagatttcc aaattgatga aaaatatcgc ggtatgggga acgagcgtac
      661 atttttaatt aaaacatatg gatgtcaaat gaatgcacat gacactgagg tcattgctgg
      721 tatacttgaa gcattaggct atcaagcaac gactgatatt aacactgcag atgttatttt
      781 aattaataca tgtgcgatta gagaaaatgc cgagaacaaa gtgtttagtg aaattggtaa
      841 tttgaagcat ttgaagaaag aacgacctga tattttaatc ggagtttgtg gttgtatgtc
      901 acaagaagag tcagtagtga ataaaatttt aaaatcgtat caaaatgtag atatgatatt
      961 tggtacacat aatattcatc atttaccaga aattttagaa gaagcatatt tatctaaagc
     1021 aatggttgtt gaagtatggt ctaaagaagg agacgttatt gaaaatcttc caaaagtccg
     1081 tgaaggcaac attaaagcat gggtcaatat tatgtatggt tgtgataagt tttgtacata
     1141 ttgcattgtt ccatttacaa gaggtaaaga acgaagccgt agacctgaag acattataga
     1201 tgaagtacgt gaacttgctc gtgaaggtta caaagaaata acgcttttag gtcaaaatgt
     1261 aaattcttat ggtaaagatt tacaggatat agaatatgac ttaggagatc ttttacaagc
     1321 aatttctaaa atagcgattc caagagttcg tttcacaaca agtcatcctt gggacttcac
     1381 agatcacatg attgatgtta tttcagaggg tggtaatatc gttcctcata tccacttgcc
     1441 agttcaatct ggaaataatg cagtattaaa aataatgggt agaaaatata cacgagaaag
     1501 ttatttggat ttagtaaaac gaatcaaaga tagaattcca aatgtagcat taactacaga
     1561 tattattgta gggtatccaa atgaatcaga ggaacaattt gaagaaactt taactttgta
     1621 tgatgaagtt ggttttgaac atgcatatac gtatttgtat tcacaacgtg atggtacgcc
     1681 tgctgctaaa atgaaagata atgtaccttt agatgtcaaa aaggaacgat tgcaacgttt
     1741 gaataaaaaa gttggtcatt attcacaaat agctatgagt aagtacgaag gacaaactgt
     1801 aacagtactt tgtgaaggta gtagtaaaaa agatgatcag gttcttgctg gctacaccga
     1861 taaaaataag ttagttaatt tcaaagcgcc taaagaaatg attggtaaac tagtggaagt
     1921 acgaatagat gaagctaaac agtattcatt aaatggcagt tttgtaaagc aagtagagcc
     1981 ggaaatggtg attcaataaa tgtataataa agatgacgtg ttgaaacaag cggataatat
     2041 tgcaaataaa attaaaaatt tggatactat caaaacttat caacaaattg aagcacagat
     2101 tcatcagaac caaacgataa agactaaaat ggatatgtta aaaaagcatc aaaaacaagc
     2161 agtaaacttt caaaattacg ggaaacaaaa tgcgctagaa cagtcggaac ataccattca
     2221 aagtatagaa gcagaaataa atacattgcc catagttgaa cagtttcaag cttcacaata
     2281 tgaagcgaat caattattga aaatgtttgt atcaacaatg gaaacacgtt taaatgacca
     2341 taataaagcc aaacatagtg attaattaca aacaaaaagg agaacaatca tatgaaatca
     2401 agaaaactgg ctataactgc acttttaatt tcaataaatg ttgtattaag cagtattatc
     2461 atcattcctc taggaccagt taaggcagca ccagtacagc attttgtaaa tgtattaagt
     2521 gcggtcatag taggtccttg gtatggatta gctcaagcgc ttatatcatc aattttaaga
     2581 gttctttttg gaactggtac agcttttgca tttccgggta gtatgattgg agttttattg
     2641 gctagtatgt tttacatata tcgtaaacat atattcatgg ccgcggtcgg tgaagtactt
     2701 ggaactggtg tcatcggaag tttaatttgt ataccattag catatttcct tgggcttcaa
     2761 gacttcttca ttaaaccgtt aatgattacg ttcatagtct caagtgctat cggatctatt
     2821 ataagttatt tcttattaat tactctaaaa aaacgtggta ttcttcaaag gtttataaaa
     2881 taattttatg atatatgatt tcaaacagag tcattgttca ttttaatatt gaacgccctt
     2941 acttaattaa atgtcttgaa tgttagaatt ttaatataat tctaaacatg ctcttaacac
     3001 attttattag gtttgaggcg ttttcttata catttaagat gcttcgttat tatatggtat
     3061 gaataggttt gtaaaagtaa gtatgttaaa atgtataagt taaataatta ataattagaa
     3121 agcttacaaa cgaatatatt gtaaagacaa attttaaaaa gagacaaggg ttgtgaagaa
     3181 ataatatgtc taatgttaca ccaatgatgc agcaatattt aaaaataaaa tcagaatacc
     3241 aagattgctt attatttttt agactaggtg atttctatga aatgttttat gaagatgcca
     3301 aggaggcatc acgtgtactt gaaattactt taactaaaag agatgctaaa aaagaaaatc
     3361 caattccgat gtgtggtgtt ccgtatcatt ctgcagatag ttatatagat acacttgtta
     3421 ataatggata taaagtagct atttgtgaac agatggaaga tccgaaacaa acgaaaggta
     3481 tggttagacg tgaggtagta agaattgtga ctccaggaac tgtgatggag caaggtggtg
     3541 tagatgataa acaaaataac tatattttaa gttttgttat gaatcaacct gaaattgcgc
     3601 ttagttactg tgatgtttct actggtgaat taaaggttac acattttaat gatgaagcga
     3661 ctttattaaa tgaaattacg acgataaacc ctaacgaagt tgttatcaat gacaatattt
     3721 ccgatcattt aaaaagacaa attaatatgg tgacagaaac aataacagtc agggaaacgt
     3781 tatcaacaga aatctatagt gtgaatcaaa ctgaacataa attaatgtat caagcgacac
     3841 aattattgct agattatatt catcatacac aaaaacgtga tttatcgcat atcgaggatg
     3901 ttgttcaata tgcagctata gattatatga aaatggattt ttatgctaag agaaaccttg
     3961 agttaacgga aagcattcga ttaaaatcaa aaaaaggaac gctactttgg ctaatggacg
     4021 aaacgaaaac accaatggga gcacgccgct taaaacaatg gatagataga ccactaataa
     4081 gtaaagaaca aattgaagca cgattagata tcgttgatga atttagtgct catttcatag
     4141 aaagagacac cttaagaaca tatcttaatc aagtgtatga tattgaacgt cttgttgggc
     4201 gtgttagtta cggaaatgtt aatgcgagag atttaattca acttaaacat tccatttctg
     4261 agataccaaa tattaaagca ttactaaatt ctatgaatca ggatactctt gtacaagtta
     4321 atcaactgga gccacttgat gatttacttg atatattaga acagagttta gtagaagaac
     4381 caccaatttc agttaaagat ggtggactat tcaaagttgg ttttaatatg caattagatg
     4441 aatatcttga agcttcaaaa aacggaaaaa catggttagc agaattacaa gccaaagaaa
     4501 gacaacgtac tggaataaaa tcattgaaaa taagctttaa taaagtgttt ggttatttta
     4561 tagaaataac acgtgccaac ttgcaaaatt ttgaaccaag tgaatttggt tatatgagga
     4621 agcaaacgtt atcgaatgcg gaacgattta taactgatga acttaaagaa aaagaagata
     4681 tcattttagg tgcggaagac aaagccattg aattagaata tcaattattt gttcagctac
     4741 gtgaagaagt taaaaaatat actgaacgtt tacaacaaca agctaaaatt atttcagagt
     4801 tagattgttt acagagcttt gcagaaattg ctcaaaaata taattatact aggccttcat
     4861 ttagtgaaaa taaaacatta gaattagtgg aatctaggca ccctgtagtg gaaagagtaa
     4921 tggattataa tgactatgtg cctaataatt gtcgattaga taacgaaaca tttatatatt
     4981 taattacagg tccgaatatg tctggtaaat cgacgtatat gagacaagtt gccataatta
     5041 gtataatggc ccaaatggga gcttatgtcc cttgtaaaga ggctgtgtta cctatatttg
     5101 atcaaatatt cactagaata ggtgcggcag atgatttggt ttcaggtaag agtacgttta
     5161 tggtagaaat gctagaagca caaaaggcat taacttatgc aacagaggat agtttgatta
     5221 ttttcgatga aattggacgt ggtacttcaa cgtatgacgg tttagcttta gcgcaggcaa
     5281 tgatagagta tgtagctgaa acatcacatg ctaaaacgtt attttcaaca cattatcatg
     5341 aattgacaac attagatcaa gcattaccaa gtctaaaaaa tgttcacgtc gctgctaatg
     5401 aatataaagg tgaacttata ttcttgcata aagtcaaaga tggtgcagtt gacgatagtt
     5461 atggtattca agttgcgaaa ttagctgatt tacctgaaaa agttattagc agagcacaag
     5521 tgatcctaag cgagtttgaa gcgtctgctg gtaaaaaatc atcgatatca aatttaaaaa
     5581 tggtcgaaaa tgaacctgaa attaatcaag aaaattcaaa cttaagtgtt gaagaaacaa
     5641 ctgatacttt atctcaaaaa gactttgaac aagcatcatt tgatttgttt gaaaatgatc
     5701 aagaaagcga gattgaacta caaattaaaa atttgaattt atctaatatg acaccaattg
     5761 aggcattggt gaagttaagt gaattacaaa atcaattaaa atagaggtgt tgcaaaatgg
     5821 ggaaaattaa agaactccaa acctcattag caaataaaat cgcagcaggt gaagtagttg
     5881 aaagaccgag ttctgttgtg aaagaactgt tggaaaatgc tatagatgca ggcgctacag
     5941 aaataagcat tgaagtagag gaatctggcg tccaatctat tcgcgtagtc gataatggaa
     6001 gcggaattga agcggaagac ttaggattag tatttcatag acatgcgact agtaagttag
     6061 atcaagatga agatttattt catattagga cattaggatt ccgtggtgaa gcactagcca
     6121 gtatttcatc agttgctaaa gtaacattga agacttgcac ggataatgct aatggaaatg
     6181 aaatatatgt agaaaatggt gaaatattaa atcataagcc tgcaaaagcg aaaaaaggaa
     6241 cagatatact tgtagaatca ttattttata atacaccagc acgtttaaaa tatattaaaa
     6301 gtttatacac tgaattaggt aaaataacag atattgtcaa cagaatggca atgagccatc
     6361 cggacattcg aatagcactc atttcagatg gcaaaacaat gttaagtaca aatggttcag
     6421 gacgaactaa tgaagtgatg gcagagattt atgggatgaa agttgcacga gatttagtac
     6481 atatatctgg agatacaagt gattatcaca ttgaaggttt tgttgcaaag cctgaacatt
     6541 ctagaagtaa taagcactat atttctattt ttattaatgg acgatacatt aaaaacttta
     6601 tgctaaataa agcgatttta gaaggctatc atacactctt aacaataggt aggttcccga
     6661 tttgttatat taatattgaa atggatccaa tcttagtaga cgtaaatgtt catccaacaa
     6721 aactagaagt gcgtttatca aaagaagagc aactatatca attgatagtg agcaaaatac
     6781 aagaagcatt taaagaccgt atattaattc ctaaaaataa cttggattat gtgccgaaaa
     6841 aaaataaagt gttacattca ttcgaacaac aaaaaatcga atttgaacaa agacaaaaca
     6901 cagaaaataa ccaagagaag acgttttcat ctgaagaaag taacagtaag ccatttatgg
     6961 cagaaaatca aaacgatgag atagttataa aagaagattc atataatcca ttcgtaacga
     7021 aaacatctga aagtttaata gctgatgatg aatcttctgg ttataataat acacgtgaaa
     7081 aagatgaaga ctacttcaaa aaacaacaag aaattttaca agaaatggat caaacatttg
     7141 attcaaatga cgatacatct gtgcaaaatt atgagaataa agcgtctgat gattattatg
     7201 atgtgaacga tattaaagga acaaaaagta aagaccctaa acgaagaatt ccatatatgg
     7261 aaattgttgg ccaagtacat ggaacgtata ttattgctca aaatgaattt ggcatgtaca
     7321 tgattgacca gcatgcagct caagaaagaa taaaatatga atattttcga gataaaatag
     7381 gtgaagttac caatgaagta caagatttat taatcccgtt aacatttcat ttttcaaaag
     7441 atgaacaatt agtcattgat caatataaaa atgagcttca acaagtaggt atcatgttag
     7501 aacattttgg tggtcatgat tatattgtaa gtagctatcc agtttggttc cctaaagatg
     7561 aagtagaaga aattattaaa gatatgattg agctaatttt ggaagagaaa aaagtagata
     7621 tcaaaaaatt acgtgaagat gtagcaatca tgatgtcatg taaaaaatct attaaagcga
     7681 accattattt acaaaaacat gaaatgtctg atttaattga tcaattaaga gaagcggaag
     7741 atccatttac atgtccacat ggtcgtccaa ttatcattaa tttttcaaaa tacgaattag
     7801 aaaaattatt taagcgtgtg atgtagagag gatgaatcaa ttgaataaca acatattgcc
     7861 tgccataaga aacattaaag atttagagaa actgattaaa acagattata aaatgtgtgt
     7921 tcttctagat atgcatatag gacatattaa aagtattatg gaattgctga agcaaaatca
     7981 tatagagtgt tttattcata tagatttgat aaaaggttta agccacgatg aatttgcaag
     8041 tgaatttatt attcagcaat acaagccaaa aggtattgta tcgactaaat ctaaagtaat
     8101 aaaaaaagcg aaatcattaa atactttaac gatttttaga gtgtttatta ttgatagtca
     8161 agcattgaaa cgcagtatag atttgataaa aaaagttgaa cctgattttg ttgaagtact
     8221 tccaggtgtt gcgagtaaag cgattcatca tattcagaaa gaaacaaaca cacaagtcat
     8281 tgcaggtggc ctaattaaca caatagatga agttaatgaa gctgttaaaa atggagcgaa
     8341 atatgtaaca actagttatg ataaactctg gtgaatcata agtctgcaaa aattatattt
     8401 ttcactagat ttagtcatat aacatattaa aaaagccgtt ttcatatggg atatgtaata
     8461 aggaatttaa ggtatcatac aaagaataat caaataacat aattaacacc tctaaaacga
     8521 ttaaaatcat taaagttata gaggtgttta tttgtaaaaa atgcaactag tcataaaaaa
     8581 tatggctgaa gaatgtcatt taaacaaaat agcgtcataa ataatttagt taggattgat
     8641 atttctagtt ttcaaattaa atcactttta taaagacttg aaaaattaaa tttgacaaaa
     8701 tttttacaaa aaccattgac aac
//
LOCUS       DAPIDM010000111         8668 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000111 DAPIDM010000000
VERSION     DAPIDM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.111"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            47..856
                     /locus_tag="RJE42_002063"
     CDS             47..856
                     /locus_tag="RJE42_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5877470.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSSKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKIGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            885..1088
                     /locus_tag="RJE42_002064"
     CDS             885..1088
                     /locus_tag="RJE42_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877471.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            1269..2060
                     /locus_tag="RJE42_002065"
     CDS             1269..2060
                     /locus_tag="RJE42_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV5877472.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            2074..3960
                     /locus_tag="RJE42_002066"
     CDS             2074..3960
                     /locus_tag="RJE42_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HDV5877473.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFLYY
                     NPVQNILITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPAFEDLKRTDASQVDGQDDTSEDDDNQSEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQELKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            4184..5527
                     /gene="brnQ"
                     /locus_tag="RJE42_002067"
     CDS             4184..5527
                     /gene="brnQ"
                     /locus_tag="RJE42_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDV5877474.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRITQQIPVIIIFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(5601..6737)
                     /locus_tag="RJE42_002068"
     CDS             complement(5601..6737)
                     /locus_tag="RJE42_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDV5877475.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQFADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVGLDTLKRTQSNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(6769..7398)
                     /locus_tag="RJE42_002069"
     CDS             complement(6769..7398)
                     /locus_tag="RJE42_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDV5877476.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(7417..7686)
                     /locus_tag="RJE42_002070"
     CDS             complement(7417..7686)
                     /locus_tag="RJE42_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV5877477.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGNDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            7849..8157
                     /locus_tag="RJE42_002071"
     CDS             7849..8157
                     /locus_tag="RJE42_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDV5877478.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            8328..8528
                     /gene="cspA"
                     /locus_tag="RJE42_002072"
     CDS             8328..8528
                     /gene="cspA"
                     /locus_tag="RJE42_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDV5877479.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT         2945 a         1252 c         1470 g         3001 t
ORIGIN      
        1 aataagttat aatataatag taaagataaa gatagaggtg gctataatgt gtggacttag
       61 aagtataaca ttaggtacaa caaatataga acagacaaaa catttcatgg ttgacatatt
      121 aggattaaat tatgaagaac ttcttgaaaa ctcaattcgt ttcggcgatg cagatataag
      181 cccaggaaca agacttcaat ttatacaagt tccaagtgag caattagaag aatctcactt
      241 tgtgggtatt ggattacgta caccaaccga ctcaggttta gaggagtatg cggaaatatt
      301 atcgagtaag gatattccat ttacaacagt taaagaatta aatggcaata aatatttcag
      361 tctcgaagat aacaatggtc atattttctc tatatattca aacgagaata attatggcgt
      421 tggtttaggc atgccttctt tcgaaagtgc ggtcaatccg ttacatcaag tgcaaggttt
      481 agggccagtg attcttaaag tgaatcatgt agatattaca ggtcaaattt taacaaatat
      541 attcggactt gaagtatttg cagaatatca acccttcgac aatgctgact atcatgtcca
      601 agtattcaaa attggaacgg gtggtctagg tggcgaaata catttaatgc ctgtagaaac
      661 cgaaatgaca atgccggaat atggcgcagt cgatcaagtt gagtttgaaa cgaaagatgc
      721 agatttcttt aatcaagcga aatcgcgctt agatgaagtg gaaataccat atcaaacgct
      781 tgagcaagat gatattgaat caattagaat tactgaaaac agtggattat cgtttatatt
      841 cactttacaa aaataatttt tttacgatag tgaggacaaa atttatgtta catgaaactt
      901 ggaaagaacg tacaccaatc aagaaagtag aagtcattaa tacagatgca aagaaattta
      961 cagtttctga catgttaacg gttggcaaac aatatgatgt tatcaacgaa actgaagaat
     1021 attatcaaat aatagataat tctggattag ttggcggtta ctataaaaca tatttcaaag
     1081 aagtttaaac gcataattat tgaaatgaag cctaatatga gattttcaat tagtaaattt
     1141 ggtgaaatat aaacaattcg catactaagc attgcgtttt agatcagaat agaatagtaa
     1201 actaaggcat ttggagaact ttttccatgt cttagttttt taatttattt aaaggagaac
     1261 gttttaaaat ggcactaaaa cattataaga attcagattc aacagttttc aatgatgcga
     1321 aggcattatt cgatttaaat aaaaatattt tacttaaagg tccaacaggt tcagggaaaa
     1381 cgaagttggc agaaacatta agtgaagttg ttgatacacc catgcatcaa gtcaactgtt
     1441 ctgttgattt agatacagaa agcttattag gctttaaaac aattaaaaca aatgcggaag
     1501 gacaacaaga aattgtcttt gtagatggcc cagttattaa agctatgaaa gaggggcata
     1561 ttttatatat tgatgaaata aatatggcta aacctgaaac attgcctgta ttaaatgggg
     1621 tcttagatta tcgtcgtcaa attacgaatc catacactgg tgaagtaatc aaagctgtac
     1681 caggatttaa cgttattgca gcgataaatg aaggttatgt tggtacattg ccaatgaatg
     1741 aagcactaaa aaatcgcttt gttgttattc acgttgacta tattgacggg gatattttaa
     1801 aaaatgtgat taaagagcaa agtttattac aagatgataa acaaatcgaa caaattatta
     1861 agtttaacga agatttacgt actatgtcta agcagggaca aatttctgaa gaagccgcta
     1921 gtatccgtgc attattagac ttgtgtgatt taatcactgt aatgccagtt gaacgtgcaa
     1981 ttaaacgtac aattattgat aaattggaag atgaacgtga acaacaagca atatataatg
     2041 ctgtagaact aaacttttaa agagggataa aacatgagtg atcgtttcat aaaatttaac
     2101 gacgaacagt tagatgccaa gcaagtaatg atgttgcaag acttagctcg actcttatta
     2161 aaaaatgaac aaacacaagt taaaatccaa aaattccttt actataatcc tgttcaaaat
     2221 attttgatta cgagttggtt ttggtcacat cgaccaagcc atatcgaaat ggctggttta
     2281 aaaacagatg ttatgttagc tgcttacggt tatcatatga tggatgtaca aattgtaaat
     2341 gaagtagtac aagataaaac ttttaaacac cctaaatttt atcaacaact ttttaaatta
     2401 ttagaagata tgcgcgtatt aaattctatt aaagttgagc gcccaagtac tgctaaatta
     2461 attgatcttc gcttagatac acggatatca tatacggaat ctcaaattaa agtgtatcgt
     2521 actaaaacac aatatacaga tttattattt ttatatttag aacatgcatt tttaagtcaa
     2581 gatttctttg atataccatc tatacattca gatttagatg atattttagt taatatgttc
     2641 ttatatttac ctaatttttt ccaaaatcaa aattctgaag ataatatgta tctggctcaa
     2701 agaattatgt atcaagtaga tgatatttta aaagaagata tgctaaacga atattattac
     2761 ttacctaaaa cactatataa tacattagct agtccagcgt ttgaagattt aaagagaact
     2821 gatgcaagtc aagtcgatgg gcaagatgat acttcagaag atgatgataa ccaaagtgaa
     2881 aaagctgact ctaaatctgc agatagtgag tcaaaaggtg gcgcctattt agaaatggag
     2941 cttcacgaag gacaaaatag tgaaacatta ggtaatgatg aagcacgtga aggcgatgct
     3001 acagacgata tgactgatat gatgaccaaa aagggcaaag gatcaaacga tacgctaaat
     3061 cgtgaagaag gcgatgctgt tggtcaaagc caggcatttc aacttgatgg tgtgaacaaa
     3121 aatgtagaaa taaaatggca aattcctgaa attgagccac aatatgtttt agaatatcaa
     3181 gaattgaaac aagacgtaca atatgaaatc aaagacttaa ttcaaattat aaagaaaacg
     3241 attgaacgag aacaacgaga tgcacgtttt aatttaacaa aagggcgatt acaaaaggat
     3301 ttaattaact ggtttatcga cgatcagtat aaattgtttt acaaaaaaca agatttaagt
     3361 aaatcatttg atgcgacgtt tacattgtta attgatgcgt cagcaagtat gcatgataaa
     3421 atggctgaaa cgaagaaagg tgtagtgtta ttccacgaga cgttaaaagc attgaacatt
     3481 aaacatgaaa ttttatcatt cagtgaggat gcctttgatt cagacgagca tgcacaacca
     3541 aatatcatta atgaaattat taactatgat tactcaacct ttgaaaaaga tggcccacgt
     3601 attatggcac ttgaacctca agatgataat cgtgatggtg ttgctattag agttgcaagt
     3661 gaacgattaa tgcgtcgaaa tcaacatcaa cgatttttaa tcgtattttc tgacggcgaa
     3721 ccgtctgcat ttaattatag tcaagatggt attattgata cgtatgaagc tgtagaaatg
     3781 tcacgtaaat ttggcattga agtatttaat gtatttttga gtcaagatcc aataactgaa
     3841 gatgttgaac aaacaattca taatatttat ggtcaatatg ccattttcgt tgaaggagtt
     3901 gcgcatttac ctggacatct ctctccatta ttaaaaaaat tactacttaa atctttataa
     3961 ataagtttaa aatatgagtt tatatgaaat tgtgaataaa agtcatcagt tactatataa
     4021 aaagtaaatt tgaagaattt tattttctgg cataatttga tttaaacaac acatgtgttt
     4081 tgattctgct atgtacaaca tatttcatat agactcttta ttattttttg tgctagaata
     4141 tttacttgta aattattttg tgttttaaaa aaggagtaac tctatgaata aaaatacatg
     4201 ggtcattggg tttacgctct tcgctatgtt ttttggcgca ggcaatctaa tttttcctcc
     4261 taatctcgga ttggatagtg gtcaattttt ctggcccgcc attttagcgt ttgttctaac
     4321 tgggattggt ttaccattat taggtgtgat tgtaggtgca cttgataaag aaggatatat
     4381 tggcgcatta aataaaattt cacctaaatt ttcaatattg ttcttaatca tcatttattt
     4441 gactatagga ccacttttcg caatacctag aactgcatct acatcttttg aaatgacaat
     4501 tacaccaatt atacatagca atagtagtat cgctttattt atatttacga ttatctactt
     4561 catagtcgtt ttgtatattt gtttaaatcc atctaagtta atcgatcgta ttggttcatt
     4621 attaacacca ttattattaa ttactatttt agcgatgatt attaaaggat acttagactt
     4681 tagcggtaat agtgctggaa agggcaatga agcactatat cattctaatt tttcaagttt
     4741 tgctgaaggc tttacacaag gctatttaac aatggatgcc attgcagcaa ttgctttttc
     4801 aatgattgtt gttaatgcag taaaactaac aggtattact aaaacaaatc aaatattcaa
     4861 acaaactttg actgctggtt tgattgcagc cgttgcttta attttcatat atatttcatt
     4921 aggttatatt ggtaatcata tgccagtaag tgacatgacg ttagatcaat tgaaatccaa
     4981 agatcgaaac attgggacat atttattaac gacaatggct tcaactggat ttggctcatt
     5041 tggaaaatat ttattgggca ttattgtggc gctggcatgt ctaactacag catgtgggct
     5101 tattgttgca gtttctgaat atttccatag aatcgtacct aaagtatcat acaaagcatt
     5161 tgtattagtt ttcattttaa tgagttttat tattgctaac caaggtttaa atgctgttat
     5221 ctcaatgtca attccggtat taagtattgt atatccagta gcaataactg ttgtattatt
     5281 aattttaatt gccaaattca taccgacaaa acgtattaca caacaaattc cagttattat
     5341 catctttata ttgtcgattt tcagtgttat tagtaagtta ggttggctga aaattaactt
     5401 tatagaatca ttgcctctaa gagcgtattc tttagagtgg ttcccagtag caattattgc
     5461 aacgatatta ggctatctag tcggcatatt tgtaaaacaa gatccaatta aatatcaaca
     5521 ggaataacga atattataaa aggaagcatc tcccatttaa tcttaggatg cttccttttt
     5581 tgttatatct aattcgtaat ttatgattgt ttattcggtc ctttgatgtt cactaaatgt
     5641 gactttaaat cttgttctaa ttgttgtaat tctttttcag ctaattgtcg ttcttctcga
     5701 ccatgttgtt gaataattaa tgtttcttct attgtctcaa taatgttact ctgtgtacgt
     5761 ttcaatgtat caaggccaac aatgccacgc tcattttctg ttgcagtttc aatcgcattt
     5821 tgtttcaaca tttctgcatt tgctgttaat aaatcattag ttgtatcagt gacagctcgt
     5881 tgtgcagcaa ctgcattacg ctgtctcatt aacgtaagtg caatggccat ttgatttttc
     5941 catagtggaa tacttgtcaa aattgaactt tgtatcttct cggcaagtgc ttgattaaca
     6001 ttttgaatca tacgaatttg tggcgcagtt tgcaaagcta tttgtcttga aagctgtaag
     6061 tcatatatgc gtttatctag tctatctata aattgttgca tatctgcaaa ttgttgaata
     6121 tccatttgat tagtggattg ctgcgcttgc tgttgcaatt gtggtagctt ttcattttct
     6181 aattgcaact ttttttgctg tgcagcaatg atatgcaatg ataaatcatc aaagtattgt
     6241 ttgtttttat catatagcgt atctaataat tcaatatctc ttgttaaatg tgtttgatgt
     6301 ttctgcagtt gaatcgttat gcgatcgact tgagcactaa ctgattgcat tcttgaaaag
     6361 atttcattga tagacgactt tgctctgcta aaaattcttt ttaacataga tggtttatca
     6421 gtatttaact catttggatt aactgacttt agttttgaca tgagatctga caaagtatct
     6481 ccaataggac caacatcttt actttgtact tcatccaaca tttgatgtga aaattgagac
     6541 atttgtttct gtaaatcaga accaaacgct aataaacctt cattgtctaa agggttaatt
     6601 tgtttactga ttgtgtctac ctgtttttgt tgttcaatag ttaacttttc tttttgaata
     6661 gtagtattcg ataattcttt atcgcttata aaatcatcta gtggatggct ttctttgaaa
     6721 cttttatttt cagtcatcaa tcatctctcc aatagttgct attttacatc aatcttgatg
     6781 atgcttttga tgtaacttat ttaattccat ttcaatatct aaacgttcat aatcatcttc
     6841 gttgagacgc tttaaatcag cgattaatgt tcgttttacc tcatccaaag taattcgtgt
     6901 ttgttctaat ttttgctgtt cattaattga ttttttgggc atttttgcta gacgtgtata
     6961 tgcatcaacc aaatttaaag cattatcaat atgagaataa aaaaagcctt caactttata
     7021 aaatgatgca ggtctctgtc taactgtcgt ataaatagaa cgtgaaattt ggtatatatc
     7081 attaatttgc ctaaaatctt taattgatct tatattgaca tacgttttta aaatacctct
     7141 aagtttttgg tgtgtatgat ttaactgatt tcgaatatag cgatagtctt ttctagtcaa
     7201 accaatttcg tttaaatatt tgcgtgaagt gagtttttgt atcggtaggt atgtcattaa
     7261 aaagccaaca ataccaatag acatatcaat taaaaaagat acatcaagtg caatcatccc
     7321 aaatatgctt gttaaaaacg ctacaggaat tcccactaac accccaaata tatgagaaat
     7381 attatatctc actgtcatct tcctttattt agcattttat attgatcgaa aatccgataa
     7441 tttttgattt agttctaact cttcaagttg atggctcgtt acatttgatg ctggtgaggc
     7501 accttcaatt acaccttgaa taaatctctc tatatctgcg tcattccctt gtgcatatat
     7561 ctctacatag tcatctacat tttgaacagt accgacaatg ttatagttca ttgcaatgcg
     7621 ttgtgtaaaa tatctaaatc cgacgccttg aacgcgtccg aatacttgta aatgtatatg
     7681 tctcattttt accacctcat aatattatta tacgtagttt tacttaaaaa aactaataat
     7741 tactatagtt aatactttgt ttgtttcaag tcgtcaaact tgattttcag aggataaagg
     7801 tataaaaata agtatagagt ttttgaagta tggaaggggt ctttaataat gtggacagtt
     7861 accaaaatta gagccgatta tgagggatgg tggttattca gtgactggcc agaaaacatt
     7921 gttgaaaaat atcaatatca agattttgat gacatgttta agcactatca acaattgatt
     7981 aatcaatgta aagttcagtt cgataattat gtcacaggca aatataatat ttatgcattt
     8041 tataataatt gtgacatgaa ctattgcgaa gattgtgaag aggatttaca aatattttat
     8101 agtttcattg ttttacaaaa taatgaagta tattataaac taccaataat tgattaaatg
     8161 aatatttttt attattgtat tgcaatttgg taataactgc actataatac aagttaagca
     8221 gatgattatt ccatattgca aagaatattt gagtgaaatt tgcatgtatt ttttgtaata
     8281 tgcgaataag catattgaat gaattttagt cttggaggtt tcagattatg aaacaaggta
     8341 cagttaaatg gtttaacgct gaaaaaggat tcggctttat cgaagttgaa ggagaaaatg
     8401 acgtattcgt acatttttca gcaattaacc aagatggtta caaatcatta gaagaaggtc
     8461 aagctgttga gtttgaagta gttgaaggcg accgcggtcc acaagctgca aacgttgtta
     8521 aactataatt cttagatttg aatcattgat tttaactaac accttaccaa gaagtaaggt
     8581 gtttttatat gcttatttca aatacttaac gcgatgagtt tctcaaccaa attgagaact
     8641 ctttttttat atcaccaaca ttgtttgc
//
LOCUS       DAPIDM010000112         8596 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000112 DAPIDM010000000
VERSION     DAPIDM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.112"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            651..1418
                     /locus_tag="RJE42_002073"
     CDS             651..1418
                     /locus_tag="RJE42_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDV5877480.1"
                     /translation="MKFVFDIDGTLCFNGKSIDPSIIDSLLQLQHAGHELIFASARPI
                     RDLLPVLPEVFHQHTLIGANGAMISQQSKISVIKPIQAYTYHHILRIIQKYELDYIID
                     DDWNYAAKLDAENAIFERLDPHKLASCINVANIDTPIKIILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHIDNNTQAICKVLKSYK"
     gene            complement(1466..1939)
                     /locus_tag="RJE42_002074"
                     /pseudo
     CDS             complement(1466..1939)
                     /locus_tag="RJE42_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            complement(2063..2764)
                     /locus_tag="RJE42_002075"
     CDS             complement(2063..2764)
                     /locus_tag="RJE42_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877481.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(2757..3530)
                     /locus_tag="RJE42_002076"
     CDS             complement(2757..3530)
                     /locus_tag="RJE42_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877482.1"
                     /translation="MSLIDIQNLTIKNTCEKCLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLRCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKSSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERLYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(3517..4347)
                     /locus_tag="RJE42_002077"
     CDS             complement(3517..4347)
                     /locus_tag="RJE42_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877483.1"
                     /translation="MHKIFSKNNLIFFVFGAFIFVMIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(4340..5326)
                     /locus_tag="RJE42_002078"
     CDS             complement(4340..5326)
                     /locus_tag="RJE42_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877484.1"
                     /translation="MFIIKSMLYRLIQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKEKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVLTLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNECY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            complement(5631..5975)
                     /locus_tag="RJE42_002079"
     CDS             complement(5631..5975)
                     /locus_tag="RJE42_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877485.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            6180..7994
                     /gene="pepF"
                     /locus_tag="RJE42_002080"
     CDS             6180..7994
                     /gene="pepF"
                     /locus_tag="RJE42_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDV5877486.1"
                     /translation="MSQGLPLREDVPVSETWDLTDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSRLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         3025 a         1435 c         1267 g         2869 t
ORIGIN      
        1 agatgtaaat gatgttaggg cttatagtta ttgatactat aggctctttt ttatgctttt
       61 taataaagcc tctaatgata tattaaatga caagtttaac aagcgattgc aataaatata
      121 gatatgcatt gcacaatcta tttgcaaatg attgagttga gtttacgttt taattcattc
      181 aatatgactc aaaacgagtc aatatgtatt atattttggt gaataaacct tagcataaca
      241 atttatgtct gattttatat gtgtgtgcaa gtgcgtgata tcaaatgtca tatttatctg
      301 attgtaattt ataaaatgca tgagcaatga ttgctgtaac aaattctagc aaagtaatca
      361 gtttgtagat tagaaataaa aatgaagata gatatattat cgatacatag atttaatatg
      421 caccaaaact tgagtattgg tttaatgaaa catagcatga atgtaaattt agtttactga
      481 cataaacacg tcacaaactt acggttatat aagtcccctt atggaattcc gtattctagt
      541 ttacataata tatattatag gaagttacat atctaataca aaaggtgccc tacattatca
      601 tttacttaca tatgctataa tcatattatt ctattgtttg gagtgatttg atgaaatttg
      661 tatttgatat tgatggtaca ctttgtttta atggtaaatc aattgatcca tcaatcattg
      721 attcattgtt acaattacaa catgctggtc atgaacttat atttgcatct gcacggccga
      781 ttcgtgattt gttaccagtt ttaccagaag tctttcatca acatacttta attggcgcaa
      841 atggcgctat gatttcacag caatcaaaga tttcagttat caagccaatt caagcttata
      901 catatcatca tatcttacga ataatacaaa aatatgaatt agattatatt atcgatgatg
      961 attggaatta tgctgctaaa ttagacgctg agaacgcgat ttttgagcgc ttagatccac
     1021 ataagctggc tagttgtatt aatgttgcaa atatcgacac accaatcaag atcattttat
     1081 taaatataga cccggcacaa attacaacta tattagacga gctaaataaa taccatcaag
     1141 aattggaaat gattcaccat tcaaatgagt ataacattga tataacagcg caaaatatta
     1201 ataaatatac tgcattacaa tatatatttg atgcagatgt taaatatata gcatttggta
     1261 atgaccacaa tgatattgtc atgttacaac atgctagtag tggctatatt ataggaccat
     1321 cagaagcata cacacacgca atattgaaac ttgataaaat caaacacatc gataataata
     1381 cacaagctat ttgcaaagtc ttaaaatcat ataaataaaa gcacccctat caaatgataa
     1441 tcattatcaa tcgatagggg ctattttaat aaaattcgtc ctcgaacatt tcttcctctt
     1501 catctaatcc aaataattct gccatttctc catgttcaat taacatgttt aaatatgcat
     1561 cgcggagttc ttcttcactc atatcattaa tcatttcttt aagactatca atccacatat
     1621 ttctgcgtaa ttgatagtct tcttcaactt cgtttaacat cattatatgt ttatttgctg
     1681 cttctagact agctgtaaag agtaatgcaa tcatatgttt acatatcact cgtcttccat
     1741 cagcatgagg acaattacat ttggattttc taggatgttc catatcaata taacaacgat
     1801 atactttgtt gccactgccc tttacttcag cctcatgcta cgtttctgaa aatgatttta
     1861 agttaatgac gcattcactt tgataataat taaagcctct ttctatagaa cgaatacttg
     1921 caatatcaag taatcccatt aatgatactc ctttttatta ttatttttaa ataaagaaaa
     1981 taaaatcgat aagtgtctag attaatatac ttgattcatc tatattttat aacaagtcta
     2041 gaattatcgc attcttaaat agctaatatg aaaatgcttg cactaattct tttgtataag
     2101 ggtgtctatc aacattaaat aattcctcta ttgcaaaatc atcgactatc atgccatcct
     2161 taagaacgat aattctatta actaagcgtt gtaacacgga taaatcatga gaaataacga
     2221 taaaatgatt taagttcgta atcgtttgcg cttttaatat attgattaca ttttgttcag
     2281 ctataacatc taaatttgaa gttatctcat cacatattaa aacgcgaggc tgtgctaata
     2341 acgaacgcat gacattaaat ctttgtaatt gtccgccact cacttcgctt ggtaatttag
     2401 tcaataattg cgcgtttaac tcaaaagtag ataaatgttg taataataat tgatcctgag
     2461 cagtattatc agttaggcct ctgtaataat ataacgcttc ttttaatgag gtctcaatcg
     2521 tccaatcagg gttaaagcta gttaaagggt gttggaaaat cggtaacaca gcattatcac
     2581 ttaagtaaat ttctccttta acaggtttaa acaagccaag aaccaatgaa gcgagcgtac
     2641 ttttaccaca gccactttcg cctaaaatac caacattttc tccatcaggt atagtaatat
     2701 tgatatcttg tagcaccatc tgctttttat tataaccaaa agtcacatgt tttaactcaa
     2761 tcatagtaat ccctctttaa ttgtgttcta tatttaatta gacgttcagt atacggatgc
     2821 aaatgctcat acttgaaatg attaatatta cctcgttcaa tgatttgacc ttcttttaaa
     2881 acataaatgt actgacaata tttcaataca tgacttaagt tatgtgtgat aataaataat
     2941 gtttgaccat gttctaatac aatatgctgt aataaatcca tcacttgatt accgttcaaa
     3001 gcatccaatg atgcaactgg ttcgtctgca atgattaatt taggctccaa catgagaacg
     3061 cttgctatgt ataagcgttc aagttggccc ccagaaagtt ggaaactata tttatttaat
     3121 atatctttgc tttgtaaatt aacccacgac aaagccttat caattttgga caaagcctct
     3181 tctttactac ttttataatg cttacgataa atcgcagtta actgtttacc taatttagta
     3241 tggtcgttaa aactttctgc ataattttgg gaaatatagc caattgtatg accataatat
     3301 tgactcaatc tactaacatt ttctccatca aattggtacg aatcatacgt gcaccttaaa
     3361 tcaaatggta aatattcaag taaggcttta gcaatcaaac ttttcccagc gccgctctct
     3421 ccaatcaagg cattaatctg ttgactaaaa attttcaaat caatcccttt aataagacat
     3481 ttctcacaag tattctttat tgttaaattt tgtatatcaa tgagactcat catattcacc
     3541 tcgttgtttc agcaatctat ctcttagcgc atctccggtt aaattaaaaa ttaaaatagt
     3601 tatagcaatg actgaagcag gtgcaatcaa cataattgga tgagacgaaa taaaatcacg
     3661 accttgttgc aacatagcgc cccactctgg tgttggcggt tgtgcaccta acccaataaa
     3721 tgatagtgaa cttatatata gaattatttt accgaaatca acgaccatca aaacgataat
     3781 agccggtata attttaggtg ttaaatgacg tattaatatt gttcttgtag gtacatgaaa
     3841 taattgtgcc atttttatat aaggcttatt catttcgcta ttaactatac ttctagtcaa
     3901 tcttgtgtaa ttcatccatt ttattactgt aattgagata actaaattcc ataaagatgg
     3961 ttgaaaaaag cttgctaaag caatcatgat gataaattct ggaatactta gaccaacatc
     4021 aataaacctt aacactaatc gttcaatcca ccctttttta tatccggcaa atagacctag
     4081 tgtaacacct atgacaacga tagctattaa tgttaaaaca gtaacaaaca atgttgaacg
     4141 tgcaccgata ataattcggg taaataaatc tctcccataa tcatcagttc ctaataaatg
     4201 caaccaacta ataggttcaa aagtttgtga taaattgact ttggttgcat tttcactact
     4261 gacaaagaat tgtagtacaa tcatcacaaa tataaatgcg ccgaatacaa aaaagataag
     4321 gttattcttt gaaaatattt tatgcatgac ggtcactact ttctgatatc aatggtgtat
     4381 tggttttgtt ttttggattt cctaattgta aacgctgctt cggatcaagt aataacgtta
     4441 ataaatcagc aatcgtattg ataataacaa cgaagaagcc aataaataac acgcatcctt
     4501 gaataacagg ataatctcga gatttaatac tatccattaa tagataacca ataccaggta
     4561 tatcaaataa attttcaatc actacagtac cacctattag actgccaagt gaaatcccta
     4621 gtaatgggat aatcggcaaa attgttggtt ttagtaaatc atgaattaaa atataacatt
     4681 cattcatacc gcgtaatctt gatgcttgta cgatattact ttgcaataac atcaataaat
     4741 tagaacgcac taaacgaatg atgtatgcac acatacctaa agatagcgtg agtacaggta
     4801 atataaactg acttagtata acgctatcta tattcattaa atttgtgaca ataaataata
     4861 aaataatacc gataaagaac gctggtaaac taattgatag agttgagatc actctaatca
     4921 ctttatccgt ccacttatga aatcgtttgg ctgctataat gccgagcggt atagatatgc
     4981 ataacgacac tactaatgtt gaaaaagata tgagtaatgt tatgggtgca tagttgaata
     5041 atatctgtgt taccggttct tttgattcaa aactttttcc taaattaaaa tgtaataaat
     5101 gattcatcca atgccaccac tgtaccaata aagaatcatt taatcccaat ttttctttgg
     5161 ttgcatttat ttgttccgtc gacacttgtg ctacatcaag atgtaatatt ttatcaacag
     5221 gattgcctgg tgataatttc attaaaatga atgtaagtgt agaaataaca aataaaacaa
     5281 ctatcatttg tatcagtcta tacaacatag actttattat gaacataata gtccccctcc
     5341 ttgtgtaagt tactaacact ttctttttac atgagaatgg cgcatgtata tgcaacttac
     5401 atattaagaa ctaacgttca ttatagtatt atccaaaaag aaattgaagt atatttaatt
     5461 ttttaacaaa atcattataa aatataatat tttgaatcaa gtcaaccatg taaaatataa
     5521 aaaaagtcaa aacaaaaaca actatagcac tgtattccac cactttcgaa ataattgtta
     5581 ctgcagtgta acttaaaagt cgatgatttt gtgcatatag ttgtcgaata ttatttttta
     5641 tctttacggc gaagttcagc gccctcataa ccgtattttt caatttgctt ttctaattta
     5701 cgcgcttttc tttctttacg ccaatttcta gtaaaatacc ataatagaaa actaattaat
     5761 aaactcatga tcgctaaaaa tgcagcgtat cctaataatg gttgatattt tatatcttga
     5821 aaatttggaa taaaaaatgc aagcacacct aatataacaa atgtaattac tgctgataca
     5881 aaccatttat ttaaaactaa gcaacagaat attgttaata aaatcattat taatgttgtg
     5941 atccataaat aattaggcat atcgaataat gtcatattca ttctcctttt atttcattac
     6001 tttccttgta tacattttat tataaatttt taaaaactta aacaatagca gtcagtttca
     6061 agcaatattc aatctactat tagaaaaatc attgttcttt gcggcatgga aatcgtaaca
     6121 ttatcgttta ggagacgaaa ttatgtataa tgaatgtatt ataccaaagg agtgattata
     6181 tgtctcaagg tttaccttta agagaagatg ttcctgtttc agaaacatgg gatttaacag
     6241 acttatttaa agatgatcaa caatattatg aaagcatcga tgcactagta caacaagcaa
     6301 atcaatttca tcatacatat gcaacaacat taaattcaat cgaacaaatt aatactgctt
     6361 tagctgaatt agaaaatatt ttaattgcct tagatcgctt aagtaattat gcagaactac
     6421 gtttaagtgt agatactagt aatattgagg cacaagtatt gagcgctaaa ttatctacta
     6481 catacggaaa aattgttagc cgattatcat ttgtagagtc agaaatactt gaattaccag
     6541 aagaaatact tcaacaatta gaagaatcat gtccatatca acactatatt aaacagttaa
     6601 ttaaacaaaa gccattccaa ttatctgcgt cggtagaaca agtattagca actttatcac
     6661 ctacgctaaa tagtccttac gatttatacg gcacgacaaa aatgctagat attacattcg
     6721 attcatttga acatgatggt acaacgtacc ctgtcgacta tgctacgttt gaaaatgatt
     6781 atgaagataa taaagatcct gagtttagac gtaaaagttt caaatcgttt agcgatggga
     6841 ttcgaaaata tcagcatact accgcggcta catataatat gcaagtacaa caagaaaaaa
     6901 ttgaagctga tttacgtgga tttgaatcag ttatcgatta tttattacat agtcaagaag
     6961 taacgcgtga tatgtttgac cgtcaaatcg atatgattat gcgtgacttg gcaccagtta
     7021 tgcagaaata tgctaaactt ttacaacgta ttcacggatt agataacatg cgttttgaag
     7081 acttgaagat ttctgtagac cctgattatg aaccagagat ttcaattgaa gactcaaaaa
     7141 attatatttt cggtgcgtta agtgttttag gtgatgacta tacaaacatg ttacgtgaag
     7201 catacgatca gcgatggatt gattttgcac aaaataaagg taaagataca ggcgcatttt
     7261 gtgcaagtcc atactttaca cattcatatg tgtttatttc ttggactggt aaaatggctg
     7321 aagcatttgt cttagcacat gaattaggtc atgcaggtca ttttacatta gctcaaaaac
     7381 atcaaccata tcttgaatca gaagcatcaa tgtactttgt tgaagcccct tctacaatga
     7441 atgaaatgtt gatggccaat tatttattta acacaagtga taatccaaga tttaagcgtt
     7501 gggttattgg ctcaatttta tctagaacat attatcataa tatggttacc catttattag
     7561 aagctgctta tcaacgtgaa gtgtatcaca aagtagatca aggtgaatct ttaaatgcgc
     7621 cgacattaaa tgaaataatg ctaaatgttt ataaacaatt tttcggagat gcagtagaca
     7681 tgactgaggg tgctgaatta acatggatgc gtcaacctca ttactatatg ggattatatt
     7741 cgtatacgta ttctgctggt ttaacaatcg gaactgtcgt ttctcaaaag attaaaaatg
     7801 aaggccaacc agctgttgat gcttggttag aaacattgaa aaaaggtggc agtgtatcac
     7861 ctgtcgaact tgcaaacatt gcgggtgtag acattactac agaacagcca cttaaatcta
     7921 caattcagta tatttctgat ttagtggatg aagttgaaaa attaacagat gaaattgagc
     7981 aagcaaataa ctaagatgcg agacaattta tagtagaaca atataaacca acaggctggg
     8041 acataaatcc ctaaaaaaca gcagtaagat aattttcaat tagaaaatat cttactgctg
     8101 ttctctattt atacaatact tcgtattgaa tggcttcgct atgcccgtct ggcacataat
     8161 tgtaaaattc tataaataga atttttgatg acgggtccct tcctagggtg ccgtctcagc
     8221 caccccaacc ggcacattgc tgtaagctga ctatatgtca gcttctgtgt tggggcccct
     8281 gtcttcgact ggcactgctc cctcaggagt ctcgccatta atactacgta ttaacatgta
     8341 attttacttt taaatacttt aaaagaataa gatactttgc ccaacttgca cataaatgta
     8401 aaattcaata aaatgaattt tctgtgttgg gtcccttcgt ataatttaat aaataccact
     8461 aaactaaatt aacgaggtgc cttatgtata aaatttataa catgactcaa cttacactac
     8521 caatagaaac ttctgttaga attcctcaaa atgacatttc gcgatatgtt aatgaaattg
     8581 ttgaaacgat acctga
//
LOCUS       DAPIDM010000113         8486 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000113 DAPIDM010000000
VERSION     DAPIDM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.113"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            391..1908
                     /locus_tag="RJE42_002081"
     CDS             391..1908
                     /locus_tag="RJE42_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877487.1"
                     /translation="MFKVHIDIYLYNKEKSEYKYQVSSYFNLVKENPKITYKKRHLQE
                     KQGVKGTTFTNKLKLLNKMYGVDVAKYQPFYNSNNPEYERGQFGERYISQRSNYEFNR
                     LQYQIIDMLSKILDKHPLPKSDNNYKHIPTIEKAILSGDSHSFYEYFEDIMKEIISME
                     NSSLKEKLLTDFTYQSQCRWYSESEKLKLQLESFMHKVLESNYYEGNKLYRMLSHAIE
                     ETINEADEDKVHFNYFKDYFLTDGGVKNWEQISEKITEFNGKVINDIQNEYNKIQFNN
                     ANRNQKLNFNYLYHCFEFSNNLVKARVNGNRGYILYFVDKYKIKMPFLDQLLINYLNQ
                     EIGQESINYNMQTLFEKERYDRSSTIEKLVATSKFKYEKDDSDLFKQLFNDVENSIDR
                     LGIYLLNNGINSNDENARYYRSFLKELSRIKSKLTPFSLEISKSSGREQHYPDDAIDD
                     KDERRKNKEETYHAFDDKSDIDSKLKNKINVSIDNLLSVKIKSNLYDISPNTKKQ"
     gene            2103..3212
                     /locus_tag="RJE42_002082"
     CDS             2103..3212
                     /locus_tag="RJE42_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877488.1"
                     /translation="MYQANIRDLITKLPQSNKTEHFLMNKFSNQDKVQQLQRQISQQL
                     DQQYNELLANEKAKLDQYVEVHHNLEPLKKEIESESINLDTDKLPDIKATMLEKAKND
                     EHFDKIEQLFDRLDQSLNGTNRLYTQLSLIGTRTHRITTKRFNVQGLPKLVQQMILPS
                     QFKKVYTIDFKSFEPSVAAYMTQDEQLIDYLNHEEGLYDALLRDLSLSKEKRVSVKRA
                     FIGSFLFGGRYSSSKFKINQEVSEINWLQVMSKFKKVIEFKEQVEKYKTMPTPYGIEH
                     DMSAFQGSSIMAIYVQTVASYIFKHILLEVYKAQCEKKTFKIIVPIHDAIMIECNDKG
                     IAQNVAQLMKDTANQLFNGEFAHVTVEALGGIDNE"
     gene            3205..3573
                     /locus_tag="RJE42_002083"
     CDS             3205..3573
                     /locus_tag="RJE42_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877489.1"
                     /translation="MNNDRGKSLQIPQSTLLKEGSIYVATLHSVYEKNFSGDIKHQFT
                     YEVELNQETHYVNRNITVKSMSHQLSIADWIKRHSNYNVNHINYDPYIDRKHLVLVGQ
                     YNGNYYIQDVAPLDEFGGVL"
     gene            3573..5189
                     /locus_tag="RJE42_002084"
     CDS             3573..5189
                     /locus_tag="RJE42_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV5877490.1"
                     /translation="MNHILEMLIKLLKVGMEAIDRKGLIAILTSSIGNDEMDDSEQAV
                     MVYNELIDKLQLNIPKDVDYRPNIYSYFGIQKKPNDTILVEMMICIFHIKRFDSELFV
                     FKDKGWQKVSEDELQGLISKMIQVLLVDYKPSLSTLKNVVDGIQKSTDIEKLVEHRQY
                     IGCGRNMFNLKTFKVVDNDLEIFPKTRLDLELDINDTITDKIPPNFKQYMLELANYDH
                     DLQYFLFQHMAVLLTADTKLRRGLFLYGTAKNGKSVYIKLVKSFFYSNDIVSKTLNEL
                     GGRFDKESLIGKRIMASDEVGKANIDEATVNDFKKLLSVEPIHADRKGRTQVEVTLDL
                     KLIFNTNAVLNFPSSHAKALERRIAVIPCEYYVEKADPDLIEKLQDEKKEIFLYLMYV
                     YKQIVKNDIEYLQNDRVTEISHDWLNFGYEFVSSKSANIAHQKACINLLRKLIEIKPG
                     SRIKVSRLNEVIRDEIKVSSQVINDLVQANFNVQSRLNNGYKYWVDLGWKETDKKDDM
                     ISFDKNENVTDDEFLYEDDLNLGWEDFDDE"
     gene            5414..7093
                     /locus_tag="RJE42_002085"
     CDS             5414..7093
                     /locus_tag="RJE42_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV5877491.1"
                     /translation="MKGKIALYSRVSTSEQSEHGYSIHEQEQVLIKEVVKNFPGYDYE
                     TYTDSGISGKNIEGRPAMKRLLQDVKDNKIEMVLSWKLNRISRSMRDVFNIIHEFKEH
                     DVGYKSISENIDTSNASGEVLVTMFGLIGSIERQTLISNVKLSMNAKARSGEAITGRV
                     LGYKLSLNPLTQKNDLVIDENEAHIVREIFDLYLNHNKGLKAITTILNQKGYRTINQK
                     PFSVFGVKYILNNPVYKGYVRFNNHQNWAVQRRSGKSDKNDVILVKGKHEAIISEDVF
                     DQVHEKLASKSFKPGRPIGGDFYLRGLIKCPECGNNMVCRRTYYKTKKSKERTIKRYY
                     ICSLFNRSGSSACHSNAINAEVVERVINVHLNRILSQPNVIKQIASSVIEELKQKHSK
                     QTEIKYDIDSLEKQKAKVKTQQERLLELFLDDEMDSEMLKAKQSEMNQQLEVLDQQIK
                     EAKQANQSQDDIPNFDKLKARLILMITRFSVYLRKATPEAKNQLMKMLIDSIEITTDK
                     QVKLVRYKIDESLIPQSLKKDWGSFFIPKFNFVINVTKKNRIENLSLLPLF"
     gene            7182..7520
                     /locus_tag="RJE42_002086"
     CDS             7182..7520
                     /locus_tag="RJE42_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877492.1"
                     /translation="MTLSKQLKTYITERFKLNYQETWACETIDAVAEDVLPEKYIKNS
                     PLEHKILNTFTYYNDELHEISIYPFLCYLDKELVAIGYLDNFDLDFIFLNDTHQVIID
                     ERYLLQKGGQ"
     gene            7614..7925
                     /locus_tag="RJE42_002087"
     CDS             7614..7925
                     /locus_tag="RJE42_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDV5877493.1"
                     /translation="MNINRYITRGINESIPLDLQILLWNMVKERDNQPHTDYLHIFRL
                     QEDENILSITHEQEQPAYKLEYHYINYVKNQNTLPKKVYVIRENDVDVFYYVMLLPEE
                     Y"
     gene            7940..8410
                     /locus_tag="RJE42_002088"
     CDS             7940..8410
                     /locus_tag="RJE42_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDV5877494.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKTACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTTENLKHIKEPYDKHTDIHLMK
                     AISESDAVILAYGAYAKRPVVVERVEQVMEMLKPHKKKVKKLINPATNEGSVAK"
BASE COUNT         3293 a         1145 c         1513 g         2535 t
ORIGIN      
        1 ataaataata taaaagagtg taccaaaaag tttaaaattt tgcctatgga agataaaatt
       61 ccattatcac cgttgttaca accagaatat gcaggagagg tacaagattt tattagtaca
      121 tatgagcagt ttatgattaa ttttggtaaa gtaatattgg atagtcaagg cataaaaata
      181 cagtttgaaa gtgaatcatt aagtagtatt caacgaggca ttcaagaaca ttgttactta
      241 aatgagcgaa caaatgacat tgatgtgact aaagaatggt atttatgcaa attttcaaat
      301 caaatattag aagaggataa aaatcaattg tataatgctt taaagcaact catgaatgat
      361 tctaaaaata aaaagcaagc ttttcaagag gtgtttaagg ttcatattga tatttatctc
      421 tacaataaag aaaaatctga atataagtat caagtatcta gttatttcaa tcttgtaaaa
      481 gaaaatccta aaataactta taaaaaaaga catttacaag agaaacaagg tgtcaagggt
      541 acaactttca cgaacaaatt aaaattattg aacaaaatgt atggtgtaga tgttgcaaaa
      601 tatcagccat tttataattc taataatcct gaatatgaaa gaggtcaatt tggtgaaaga
      661 tacatttctc aaagatctaa ctatgaattt aatagacttc aatatcaaat tatagatatg
      721 ttatcgaaaa tacttgataa gcatccatta ccaaagtcag ataataatta taaacatatt
      781 ccaactattg aaaaagcaat attaagtggg gattctcata gtttttatga gtattttgaa
      841 gatataatga aagaaattat aagtatggaa aattcttctt taaaggaaaa attattgact
      901 gattttacat atcaatctca atgtagatgg tactctgaaa gtgaaaaatt aaaattgcaa
      961 cttgaaagtt ttatgcataa agttttggag agcaattatt atgagggtaa caaattatat
     1021 agaatgttgt ctcatgcaat tgaagaaaca atcaatgagg ctgatgaaga taaggttcat
     1081 ttcaattatt ttaaagatta ttttttgaca gacggtgggg tgaagaattg ggagcagatt
     1141 agcgagaaaa ttactgaatt caatggtaaa gttattaacg atatccaaaa tgaatataat
     1201 aagattcaat ttaataacgc aaatcgaaat cagaaactaa actttaatta tttataccat
     1261 tgctttgagt ttagtaataa cttagtaaaa gcaagggtaa atggaaatag aggttacatt
     1321 ttatattttg tagataaata taaaataaaa atgccctttt tggatcaatt attaatcaat
     1381 tatttgaatc aagaaatagg tcaggaaagt attaattata atatgcaaac attgtttgaa
     1441 aaagaaagat atgatagaag tagtaccatt gaaaaattag tagcaacaag caaatttaag
     1501 tatgaaaaag atgattcaga tttattcaaa caacttttca atgatgttga aaattcaata
     1561 gacagattag gtatttactt actaaataat ggtataaatt cgaatgatga aaatgcaaga
     1621 tattatagat cgtttttaaa ggaacttagt agaataaaaa gtaaattaac gccattttct
     1681 cttgaaataa gtaagtctag cggaagagag caacattatc ctgatgatgc tattgatgat
     1741 aaagatgaga gaagaaaaaa taaagaagaa acatatcatg cttttgatga taaaagcgat
     1801 attgactcca aattaaagaa taaaataaat gtttctatcg ataatttatt gtcagttaaa
     1861 attaagagca atttgtatga tattagtcca aatacaaaaa aacaataact ataatgagcc
     1921 aactcaagaa tgagttggct ttttaattgt aattttacgc ttccattaca ggaatcaatg
     1981 ttatggaagc cattaagcaa ttaatttgag tcatttatag tataggtaca acgttgtgat
     2041 gttcacatga taatcagccc gggagattat tgtgtaataa cttatagaag gagcaaatca
     2101 ccatgtatca agcaaatata cgtgatttaa ttacaaaatt gcctcaaagc aataaaacag
     2161 aacacttttt aatgaacaaa ttttcaaatc aagataaagt tcagcagcta caaagacaaa
     2221 ttagccaaca gctagatcaa caatataatg agcttttggc taatgaaaaa gctaagctag
     2281 accaatacgt ggaagtacac cataatttag aaccattaaa gaaagagatt gaatcagaat
     2341 ctattaacct tgataccgat aaattacctg atatcaaagc gacaatgctt gaaaaggcta
     2401 agaacgatga acattttgat aaaatcgaac agctatttga tagattagat cagtcattaa
     2461 atggtacgaa tcgattatat acgcaattat cgttgattgg cacacgaaca catcggatca
     2521 caacgaaaag atttaatgtt caaggcttgc ctaaattagt ccaacaaatg attttacctt
     2581 cgcaatttaa aaaggtttat acaatagatt ttaaatcatt cgaaccatca gttgctgcgt
     2641 atatgacaca agacgaacaa ctgattgact acttgaatca tgaagaaggg ttatacgatg
     2701 cattactgag agacttatct ttgtcaaaag agaagcgcgt gagtgtgaaa cgtgcattta
     2761 tagggtcatt tctttttggc ggtcgttata gtagctctaa attcaaaatc aatcaagagg
     2821 ttagtgaaat taactggcta caagtaatga gcaaattcaa gaaggtcatt gaatttaagg
     2881 agcaagtcga aaaatataaa acaatgccta cgccttacgg cattgaacat gatatgagcg
     2941 catttcaagg tagtagtatt atggcaattt atgtacaaac ggtagcaagc tatattttca
     3001 agcacatttt gttggaagtg tacaaagcac agtgcgaaaa aaaaacgttc aagattatag
     3061 tgccgataca cgatgcgatt atgattgaat gtaatgataa ggggattgca caaaatgtag
     3121 cacagctcat gaaagataca gctaatcaac tgtttaatgg tgaatttgca catgtgacag
     3181 tggaagcttt aggaggtata gacaatgaat aatgatagag gaaaaagtct tcaaattccc
     3241 caaagtacat tgttaaaaga aggatctata tacgttgcta cgttacattc tgtgtacgag
     3301 aagaacttct caggtgatat taaacatcag tttacgtatg aagtagaact taaccaagaa
     3361 acgcactatg taaatcgcaa tattaccgta aaatctatga gtcaccaatt atcaattgct
     3421 gattggatta aacgtcacag taactataac gtaaaccaca ttaattatga tccatacatt
     3481 gatcgaaaac atttggtcct tgtagggcaa tataacggaa attattatat tcaagatgta
     3541 gcaccattag atgaatttgg aggggtattg taatgaatca tattttagaa atgttaataa
     3601 aattattaaa agtgggtatg gaggcaatcg accgtaaagg tctgattgcc atcctaacaa
     3661 gtagtattgg aaatgatgaa atggatgatt ctgaacaagc tgtaatggtg tataacgagc
     3721 ttatcgataa gctacagctt aacattccta aagatgtcga ctatagacct aacatataca
     3781 gttattttgg tattcaaaaa aagccaaatg acacaatatt agtggaaatg atgatatgta
     3841 tttttcatat caagcgtttt gattcagagt tgtttgtttt caaagataaa ggttggcaaa
     3901 aggtaagtga agatgaattg caagggttga tatctaaaat gatacaagtg ttgctagttg
     3961 attataaacc ttcactaagt actttgaaaa acgtagtaga tggcatacag aaatcaacgg
     4021 acatagaaaa acttgttgag catagacagt acattggttg tggacgaaat atgttcaatc
     4081 taaagacctt taaagtggtt gataatgacc ttgaaatatt ccctaaaaca cgcttagatt
     4141 tggaattaga tataaatgac acgattacgg acaagatacc cccgaatttc aaacaatata
     4201 tgttagagtt ggcgaattat gaccatgatt tacaatattt ccttttccaa catatggcag
     4261 tgttattgac ggcagatact aaactacgtc gtggactttt tttgtatgga actgcaaaaa
     4321 atgggaaatc ggtctatatt aaattagtta agtcattctt ttatagtaat gatatcgtat
     4381 ctaaaacact taatgaactt ggcgggcgtt tcgataagga aagtctaatt ggtaaacgaa
     4441 ttatggcaag tgatgaagtg gggaaagcta atattgatga agcaactgtg aatgatttca
     4501 aaaaattact atctgttgaa ccaattcatg ctgaccgtaa aggaagaacg caagtagaag
     4561 ttactttaga tttaaaactc atttttaata cgaatgctgt actcaatttt ccatcatcac
     4621 atgcaaaagc attagagcgt agaattgctg ttattccatg tgaatattat gttgaaaaag
     4681 ctgaccctga cttaattgaa aagttacagg atgaaaagaa agaaatcttt ctttacttga
     4741 tgtatgtgta taagcaaatt gtaaaaaatg atatcgagta cctccaaaat gatcgtgtta
     4801 ctgaaatttc tcatgattgg ttaaattttg gatatgaatt tgtttctagt aaatcagcaa
     4861 atattgcaca tcagaaagcg tgtattaatt tactcagaaa acttatagaa atcaaaccag
     4921 ggtcacgtat caaagtgtct aggctaaatg aggttattag agatgaaatt aaagtaagct
     4981 ctcaagttat taatgatttg gttcaagcta actttaatgt acaaagtaga ctaaataatg
     5041 gttataagta ttgggtcgat ttaggatgga aagaaactga taaaaaagat gacatgattt
     5101 cattcgataa aaatgagaat gtaacagatg atgaattctt atacgaagat gatttgaact
     5161 taggttggga ggactttgac gatgaataat gaacaaattg aagcatttgt agaagtgctt
     5221 gtgcctatca tagaagaacg tatcaataaa ggtaagtaat ctaattacgt actacaggca
     5281 gttgcctgta gtactcatat gattaagtgg taaaagtgat aaaaatgaaa cgaaattata
     5341 aatatatatt atctatatgt tgttacaaga ccgatggtct gtagcaataa tctaataaaa
     5401 ggagcggtat gatatgaagg gtaaaattgc actttattca cgcgttagta cgtcagagca
     5461 atcggagcat gggtactcaa tccatgagca agaacaagta ctcatcaaag aggttgtgaa
     5521 aaatttccca ggttatgact atgagacata tactgactca ggcatttcag gtaaaaatat
     5581 tgaaggtcgt ccggcaatga aacgtctatt acaagatgtt aaggataata aaatcgaaat
     5641 ggtattaagt tggaaattga atcgtatttc tcgctcaatg agagacgtgt ttaatattat
     5701 tcatgaattc aaagaacatg acgtagggta taaatcaatt tctgagaata ttgacacatc
     5761 caatgcttct ggagaagtac tcgttacaat gtttgggtta ataggatcta tagaacgcca
     5821 gactttgatt tccaatgtga aactttctat gaatgctaag gcacggagcg gagaggcaat
     5881 caccggtcgt gttttaggct acaaattatc acttaatcca ttgacacaga aaaatgattt
     5941 agttattgat gaaaatgaag ctcatattgt acgggaaatc tttgatttat atttgaatca
     6001 caataaagga cttaaagcaa tcacgacaat tctaaatcaa aaaggatatc gcaccattaa
     6061 tcaaaaacca ttttcagtgt ttggcgtgaa atatattttg aataatccag tctataaagg
     6121 ctatgtcaga ttcaataacc atcaaaattg ggctgttcag cgaagaagtg gtaaaagtga
     6181 taaaaatgat gtgatattgg tcaaaggtaa gcatgaagcc attataagtg aagatgtatt
     6241 tgatcaagtt catgagaaac tagcttctaa aagttttaaa ccgggtcgac ctattggtgg
     6301 agatttctac ttacgtggcc ttattaaatg cccagaatgc ggaaataata tggtatgtcg
     6361 acggacgtat tataaaacga aaaagtccaa agaacgcaca atcaaacgct attacatttg
     6421 ttcattattc aatcgctcag gaagttctgc ctgtcatagt aatgcgatta atgctgaagt
     6481 cgtcgaacgc gtaatcaatg ttcatttgaa tcgtattctt tcacaaccta atgttattaa
     6541 gcagattgcg tcaagtgtga tagaagaact gaaacaaaag catagtaaac aaacagaaat
     6601 aaaatatgat attgatagtc tagaaaaaca aaaagcaaaa gttaaaacac aacaagaacg
     6661 attattggaa ttgttcttag atgatgaaat ggatagcgaa atgttaaaag ctaaacaaag
     6721 tgaaatgaat caacagttag aagtattaga ccaacaaatt aaagaagcaa aacaagcaaa
     6781 tcaatcacag gatgatatac ccaattttga taagttaaaa gcacgactca ttttgatgat
     6841 aacacgattc agtgtgtact taagaaaggc tacacccgaa gctaaaaatc aacttatgaa
     6901 aatgttaatt gattcaattg aaattacgac agataaacaa gtaaaacttg taaggtataa
     6961 aattgatgaa agtcttatcc ctcaatcttt gaaaaaagat tgggggtctt tttttatacc
     7021 taaatttaac tttgtgataa atgtcacaaa gaaaaatagg attgaaaatt tatcactttt
     7081 accacttttt tagagtgaca aaagtggagg agttttgaaa tattttataa atatatattt
     7141 tatttatgga gtacacatta ttaattaagg aggtcattat aatgacgcta agcaaacaac
     7201 ttaaaacgta tatcactgaa cgatttaaat taaattatca agaaacttgg gcttgtgaaa
     7261 ccatagatgc ggtggctgaa gatgtattac ctgaaaaata tattaaaaat agtccacttg
     7321 aacataaaat tttaaatact tttacctatt acaatgatga attacatgaa atcagcattt
     7381 accctttttt atgttatcta gataaggaat tagtagcaat aggttattta gataattttg
     7441 atttagactt tatattttta aatgacactc atcaagttat tattgatgaa cgctacttgt
     7501 tacaaaaagg gggccagtaa ttatgaattg gatcaaggtc gctcaactat ctgtcacagt
     7561 tatcaatgaa gtcattgaga tcatgaaaga aaaacagaat ggagaaaaat agtatgaaca
     7621 tcaatcgata catcacaaga ggaattaatg aaagtattcc actagacctt caaatcttac
     7681 tttggaatat ggtaaaagaa cgggacaatc aacctcatac agattaccta cacattttta
     7741 gactacaaga agatgagaat atactctcaa tcacacatga acaagaacaa cccgcatata
     7801 aattggaata tcactatata aactatgtaa aaaatcaaaa cacattacct aagaaagtct
     7861 acgtcatacg agaaaatgat gtagatgttt tttattatgt aatgctttta ccagaagaat
     7921 actaaaagga gtgacaatga tgaatacaat caaaagtacg atacacacag aagctatatt
     7981 tagcgatgat gaacaacacc gctacttact caaaaagacg tgggatgaaa agaaaaccgc
     8041 ttgtacagtg ataaccatgt atcctcattt ggatggcgta ttatcactcg atcttacaac
     8101 tgttcttatc ctcaatcaat tagcgaattc tgaacaatat ggcgctgtat atcttgtaaa
     8161 tctattctct aatattaaaa ctacagagaa tcttaaacat attaaagaac cttatgataa
     8221 acacaccgac attcatttaa tgaaagcgat aagtgaaagt gacgcagtga ttttagccta
     8281 tggtgcttat gcgaagcgtc ccgttgttgt cgaacgcgtt gagcaagtga tggaaatgtt
     8341 aaaacctcat aaaaagaaag taaaaaagct cataaatccg gcaacaaatg aaggttctgt
     8401 tgcaaagtaa aaaatatagc taaccactaa tttatcatgt cagtgttcgc ttaacttgct
     8461 agcatgatgc taatttcgtg gcatgg
//
LOCUS       DAPIDM010000114         8407 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000114 DAPIDM010000000
VERSION     DAPIDM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.114"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            8..448
                     /locus_tag="RJE42_002089"
     CDS             8..448
                     /locus_tag="RJE42_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDV5877495.1"
                     /translation="MVQLNIANLGTRVPDKYFRQKHIIFKSFNFEKHGKFWNKWFYVR
                     KWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAELIHWISILPVIIFIKGP
                     GLVKYINVFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGERHD"
     gene            441..1934
                     /locus_tag="RJE42_002090"
     CDS             441..1934
                     /locus_tag="RJE42_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5877496.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQTIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGTRVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYHQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVSDKINAREAKNRK"
     gene            1940..3067
                     /gene="crtQ"
                     /locus_tag="RJE42_002091"
     CDS             1940..3067
                     /gene="crtQ"
                     /locus_tag="RJE42_002091"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDV5877497.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIQQQQVPVDVIVMNDGSTDETARVACSYGATVVDVVDDADGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSLSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            3104..3967
                     /locus_tag="RJE42_002092"
     CDS             3104..3967
                     /locus_tag="RJE42_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDV5877498.1"
                     /translation="MTMMAMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFHSDRRIMRALQHVAQYKNIAFQ
                     SFYNLIDTVYKDQQFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGGNNHYIDLWEYYAAI
                     AEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            3979..5487
                     /locus_tag="RJE42_002093"
     CDS             3979..5487
                     /locus_tag="RJE42_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV5877499.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNTNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTMCGENYEDYIELRQLRYIYDVYFDRDDRITVPTDL
                     AELQHMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            5914..7068
                     /locus_tag="RJE42_002094"
     CDS             5914..7068
                     /locus_tag="RJE42_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV5877500.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEVFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLMYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKARRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQQLDEAAIRLTELAYLYK"
     gene            7091..8089
                     /locus_tag="RJE42_002095"
     CDS             7091..8089
                     /locus_tag="RJE42_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV5877501.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVTQAINVVRHFNQIQTKVREHDFRWEPTILSKAIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(8179..8385)
                     /gene="copZ"
                     /locus_tag="RJE42_002096"
     CDS             complement(8179..8385)
                     /gene="copZ"
                     /locus_tag="RJE42_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDV5877502.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSAEVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT         2855 a         1243 c         1707 g         2602 t
ORIGIN      
        1 ttggttaata gttcaactaa atatagcaaa tttaggtaca agagttcctg acaagtattt
       61 tcgtcagaag catataatat ttaaatcatt taactttgag aagcatggaa aattttggaa
      121 caaatggttt tacgtaagaa aatggaaaca taagatttta gatggtcatc agcttaatca
      181 aaatatatat gatcagcgtc atttaatgac aatcaatact gatgaaattg aaaaaatgat
      241 tatagagaca aagagggcag agttgatcca ttggatatcg atacttccag taatcatatt
      301 cattaaaggc cctggtttag taaagtatat aaatgttttc tatgcaatga tagctaatgt
      361 tccaatcatt attgtgcaac gttataatcg accgagatta acgcagttac tacgcatatt
      421 aaaacgaaga ggtgaacgtc atgactaaac atatcatcgt tattggtggt ggcttaggtg
      481 ggatttctgc agcaattcga atggcacaaa gtggctattc ggtctcacta tatgaacaaa
      541 ataatcatat aggaggcaaa gtgaatcgtc atgaatcaga tggctttggc tttgatttag
      601 gtccatctat tttaacgatg ccttatattt ttgaaaaatt attcgaatat agcaagaagc
      661 aaatgtcaga ctacgttaca atcaagcgat tgccacatca atggcgtagc ttttttccag
      721 atggaacgac tatcgatttg tatgaaggta ttaaagaaac aggtcagcat aatgcgatat
      781 tgtcgaaaca ggatatagag gaattgcaaa attatttgaa ttatacaaga cgaatcgatc
      841 gtattactga aaaagggtat tttaactatg gtttagatac actatctcaa actattaaat
      901 ttcatgggcc attaaatgca cttattaatt atgattatgt acatactatg caacaggcca
      961 tagacaagcg tatctcgaat ccatacttgc gacaaatgtt aggctatttt atcaaatatg
     1021 taggttcttc atcatacgat gcgccagctg tattatctat gttattccat atgcaacaag
     1081 agcaaggcct ttggtatgta gaaggtggca tccatcattt agccaatgcc ttggaaaagc
     1141 tagcgcgtga agaaggtgtc acaattcata caggtacacg tgtggacaat attaaaacat
     1201 atcaaagacg tgtgacgggt gtcagattag atacaggtga gtttgtaaag gcagattata
     1261 ttatttcaaa tatggaagtc atacctactt ataaatattt acttcacctt gatactcaac
     1321 gattaaacaa attagagcgg gaatttgagc cagcaagctc aggatatgtg atgcatttag
     1381 gtgttgcttg ccaatacccg caattagcac atcataattt cttttttacg gaaaatgctt
     1441 atctcaatta tcatcaagtt tttcatgaaa aggtattgcc agatgatccg accatttatc
     1501 tggtaaatac gaataaaact gatcacacac aagcaccagt aggttatgaa aatatcaaag
     1561 tcttaccaca tattccatat attcaagatc agccttttac aactgaagat tatgcgaagt
     1621 ttagggataa aattttggat aaattagaaa aaatgggact tactgattta agaaaacaca
     1681 ttatttatga agatgtttgg acaccggagg atattgaaaa aaattatcgt tctaatcgtg
     1741 gtgcaatata tggtgttgta gcagataaaa agaaaaacaa agggtttaaa tttcctaaag
     1801 aaagtcagta ttttgaaaac ttgtactttg taggtggatc agtaaatcct ggtggtggca
     1861 tgccaatggt tacattaagt gggcaacaag tctcagacaa aataaacgcg cgagaagcga
     1921 agaataggaa gtgatatcta tgaaatggtt atcacgaata ttaacagtaa tagtgaccat
     1981 gtctatggcg tgtggtgcat tgatatttaa tcgtaggcat cagctaaagg cgaaaacgct
     2041 gaatttcaat cataaagcat taacaattat tattccggct agaaacgaag aaaaaagaat
     2101 aggtcattta ctacattcga tacaacaaca gcaagttcca gtagatgtca ttgttatgaa
     2161 tgacggatcg acagatgaaa cagcacgtgt agcgtgttca tatggtgcga ctgtagtaga
     2221 tgttgttgat gatgctgacg gcaaatggta tgggaaatca catgcttgtt atcaaggtgt
     2281 gacgcatgca tgtacgaatc gtattgcttt tgtagatgct gatgtaactt tcttaaggaa
     2341 ggatgctgtt gaaacgttga ttaatcagta tcaattacaa ggtgaaaaag gattgttaag
     2401 cgtacagcct tatcatataa caaagcgttt ctacgaaggg ttttcagcga tatttaattt
     2461 aatgacagtc gttggtatga atgtattttc taccttagac gacggtcgga ctaaccagca
     2521 tgcatttgga ccggtgacat taacaaataa agaagattat tatgcaactg gaggtcataa
     2581 aagtgcgaac cgtcatatta ttgaaggatt tgctttagga agtgcatata cttcactatc
     2641 attacccgta acagtttatg aaggatttcc atttgttgca tttcgcatgt atcaagaagg
     2701 atttcagtca ttacaagaag gatggacaaa gcatttgtca actggggcag gtggcacaaa
     2761 gcctaagatc atgacagcaa ttgtattgtg gttgttcggt tctattgcga gtattttggg
     2821 gctatgtctt agtttaaaat atcgccaaat gtctgtagga aaaatgttaa cagtttactt
     2881 gagctatact acacaattta tttatctgca tcgaagggtc ggccaatttt ctaatttatt
     2941 aatggtatgt catccattgt tatttatgtt ttttactaaa attttcatcc aatcttggaa
     3001 acaaacgcat cgttatggtg tagttgaatg gaaaggtcgt caatattcta tatctaaaga
     3061 acaataaatc aaggtaatgg catttcaata taggaggact agtatgacaa tgatggctat
     3121 gaattttaaa tattgtcata aaatcatgaa gaaacattca aaaagctttt cttacgcttt
     3181 tgacttgtta ccagaagatc aaagaaaagc ggtttgggca atttatgctg tgtgtcgtaa
     3241 gattgatgac agtatagatg tttatggcga tattcaattt ttaaatcaaa taaaagaaga
     3301 tatacaatct attgaaaaat acccatatga acatcatcac tttcatagtg atcgtagaat
     3361 catgagggct cttcagcatg ttgcacaata taaaaatatc gcctttcaat ctttctataa
     3421 tctcattgat actgtatata aagatcaaca atttacaatg tttgaaacgg acgctgaatt
     3481 attcggatat tgttatggtg ttgctggtac agtaggtgaa gtattgacgc cgattttaag
     3541 tgatcatgaa acacatcaga catacgatgt cgcaagaagg cttggtgaat cgttgcaatt
     3601 gattaatata ttaagagatg tcggtgaaga ttttgaaaat gaacggatat attttagtaa
     3661 gcaacgatta aagcaatatg aagttgatat tgctgaagtg taccaaaatg gtggtaataa
     3721 tcattatatt gacttatggg aatattatgc agctatcgca gaaaaagatt ttcgagatgt
     3781 tatggatcaa atcaaagtat ttagtattga agcacaacca atcatagaat tagcagcacg
     3841 tatatatatt gaaatactgg acgaagtgag acaggctaac tatacattac atgaacgtgt
     3901 ttttgtggat aagcggaaaa aagcaaagtt gtttcatgaa ataaatagta aatatcatag
     3961 aatataggtg gttgaataat gaagattgca gtaattggtg caggtgtcac aggattagca
     4021 gcggcagccc gtattgcttc tcaaggtcat gaagtgacga tatttgaaaa aaatactaat
     4081 gtaggtgggc gtatgaatca attaaagaaa gacggcttta catttgatat gggtcccaca
     4141 attgtcatga tgccagatgt ttataaagat gtatttacaa tgtgtggtga aaattatgaa
     4201 gattatattg aattgagaca attacgttat atttacgatg tgtattttga ccgcgatgat
     4261 cgtataacgg tgcctacaga tttagctgaa ttacagcaca tgctagaaag tatagaacct
     4321 ggttcaacgc atggttttat gtccttttta acggatgttt ataaaaaata tgaaattgcc
     4381 cgtcgctatt tcttagaaag aacgtatcgc aaaccaagtg acttttataa tatgacgtca
     4441 cttgtgcaag gtgctaagtt aaaaacgtta aatcatgcag atcagctaat tgaacattat
     4501 attgataacg aaaagataca aaagctttta gcgtttcaaa cgttatacat aggaattgat
     4561 ccaaaacgag gcccgtcact atattcaatt attcctatga ttgaaatgat gtttggtgtg
     4621 cattttatta aaggcggtat gtatggcatg gctcaagggc tagcgcaatt aaataaagat
     4681 ttaggcgtta atattgaact taatgctgaa attgagcaaa ttattattga tcctaaattc
     4741 aaacgggccg atgcgataaa agtgaatggt gacataagaa aatttgataa aattttatgt
     4801 acggctgatt tccctagtgt tgcggaatca ttaatgccag attttgcacc tattaaaaag
     4861 tatccaccac ataaaattgc agacttagat tactcttgtt cagcattttt aatgtatatc
     4921 ggtatagata ttgatgtgac agatcaagtg agacttcata atgttatttt ttcagatgac
     4981 tttagaggca atattgaaga aatatttgag ggacgtttat catatgatcc ttctatttat
     5041 gtgtatgtac cagcggtcgc tgataaatca cttgcgccag aaggcaaaac tggtatttat
     5101 gtgctaatgc cgacgccgga acttaaaaca ggtagcggaa tcgattggtc agatgaagct
     5161 ttgacgcaac aaataaagga aattatttat cgtaaattag caacaattga agtatttgaa
     5221 gatataaaat cgcatattgt ttcagaaaca atctttacgc caaatgattt tgagcaaacg
     5281 tatcatgcga aatttggttc ggcattcggt ttaatgccaa ctttagcgca aagtaattat
     5341 tatcgtccac aaaatgtatc gcgagattat aaagatttat attttgcagg tgcaagtacg
     5401 catccaggtg caggcgttcc tattgtctta acgagtgcga aaataactgt agatgaaatg
     5461 attaaagata ttgagcaagg cgtgtaaggg agtagtctga gagaaagatg tgagaaaagt
     5521 ataaggggaa cgtaaaaggc atagtgaaat aagtatgtgc ttaagaagtt ttatttaaca
     5581 atgaatgacg cctgttaagg ttcacgtttg aatacgtacc ttgatgggcg ttatacttta
     5641 tataagcata tctaactggt tgcgcatgcg gatatgcatg gcagttaaat cctaacgatt
     5701 gagtgtatat taatctgaaa attcctaata ttgacaataa tactgtacct acctaaaatg
     5761 aaaataaatt cattcaatac aacgtgtgtc ataaattctg ttgaacataa cggtgaatgg
     5821 cgatgtcatt gtaaccatct aaatgagagt gatacaagtt gttaggatta tgatagtcgc
     5881 ccaggctatg tttaatgata gaggaggaat ttgatgattt ctaacaaatt agcaaatatc
     5941 cctgatagtt atttcgggaa aacgatggga cgtaaaatcg aacatggacc attgccatta
     6001 attaatatgg cagttgggat ccctgatggg cccacgccac aaggtattat tgatcatttt
     6061 caaaaagcac taactattcc tgaaaatcaa aaatatggtg cttttcatgg taaagaagtg
     6121 ttcaagcaag ctattgtaga tttttatcaa agacaatata atgtgacgtt agataaagaa
     6181 gatgaagtat gtattttata tggtacaaaa aatggattag tggcagtacc gacatgtgtt
     6241 attaacccag gagactatgt attactacct gacccaggct atactgatta tttagcgggt
     6301 gtacttttag ctgatggcaa gccagttccg cttaatttag aaccgccaca ttatttgcca
     6361 gattggtcca cagttgattc acaaataata gataaaacaa aactcattta tttaatgtat
     6421 ccaaataatc caactggatc gacagctacg aaagaagttt ttgatgaagc gatagctaaa
     6481 tttaaagata cagacactaa aatcgtgcat gattttgctt atggtgcttt cggtttcgac
     6541 gctaaaaatc ctagtatact tgcttcggaa aatgggaaag atgttgcgat tgaaatttat
     6601 tccttgtcta aaggttataa catgtcaggt tttagagtcg gttttgcagt tggtaataaa
     6661 gatatgattc aagctttgaa aaagtaccaa acgcacacaa atgctgggat gtttggtgca
     6721 ttacaagatg ccgcaatata tgctttgaat cattatgatg attttttaga agaacaaagc
     6781 aatgtattta aagcacgtcg tgaccgattc gaagccatgc ttgcaaaagc tgatttgcca
     6841 tttgttcatg ctaaaggtgg catatatgta tggttggaaa caccgcctgg ttatgacagc
     6901 gagcaatttg agcaattttt agtgaaagag aagtcaatac ttgttgctcc agggaaacca
     6961 tttggagaga atggtaatcg ctatgtgaga atctcattgg cgttagatga ccagcaatta
     7021 gacgaagctg caataagatt aacagaatta gcatatttat ataaataata aagttaaggg
     7081 tgatgttaaa atgacaaaaa ttaaaatcat gagtgtacgt gatgaagata tgccttatat
     7141 caaggcgtgg gcagagaaac atcatgttga ggtagatatt actaaagaag cattaactga
     7201 tgacaatgtt gaaggtgtcg ctggttatga tggcttatcg ctatcacaac aaataccatt
     7261 atcagagcat gtttataaac gattaaatga actaggtatt aaacaaatcg cacaacgtag
     7321 tgctggattt gatacatatg atttggagtt agcaaacaag tataacttga ttgtgtcaaa
     7381 tgtgccgtca tactctccga attcaattgc ggagtttgct gtgacccagg ctattaatgt
     7441 ggttcgtcat tttaatcaaa ttcaaacgaa agtgcgagag catgatttta gatgggaacc
     7501 tactatttta tcgaaagcca ttaaagattt aaaagtagct gttattggta caggtcgaat
     7561 tggccgtgta gtagccgata tatttgccaa tggttatcaa agtgatgtgg tcgcatatga
     7621 cccatttcct aatgctaaaa ttgcaacgta tgtcgattac aaagatacga ttgaggaagc
     7681 ggttgaaggt gctgatatcg tgacattaca tgtacctgca acgaaatata atcattatct
     7741 atttaatgct gaattattta aacattttaa aaagggcgca gtatttgtca attgtgcgag
     7801 aggttcttta gtagatacta aggcgttatt agacgcatta gacaatggtg tgattaaagg
     7861 tgcagcactt gatacgtatg aatttgaacg caaacttttc ccaagtgatc aaaggggcaa
     7921 aacactgaac gatccattgt tagaatcgtt gattgacagg gaagatgtca tattaacacc
     7981 acatattgcg ttttatactg aagctgcagt tgaaaatcta attgtcgatg cattagatgc
     8041 aacattagat gtattgcaga ctggagatac taggttacga gtaaattaaa aatcgaactg
     8101 atgagataat ttggattgtt gggattctca tcagttcgat tttttaattt ggtgttgatg
     8161 acgttgaata ttgcctaatt aaacgacatc gtaaccttga tcttcaattg cgtctttcat
     8221 ttgagataca gcaactttac tgtcatcata ttgaacactt acttgaccat tttcaaggtt
     8281 aacttcagct gaagtgacac cgtcaatatt atttaatgca gattcaacag cacttttgca
     8341 gtgaccacag ctcatacctt caacatttaa aatttcttgt gacatagcat tcacctcctt
     8401 ttagagc
//
LOCUS       DAPIDM010000115         8102 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000115 DAPIDM010000000
VERSION     DAPIDM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.115"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(183..1499)
                     /locus_tag="RJE42_002097"
     CDS             complement(183..1499)
                     /locus_tag="RJE42_002097"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDV5877503.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDTINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKIDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNHQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1551..2375)
                     /locus_tag="RJE42_002098"
     CDS             complement(1551..2375)
                     /locus_tag="RJE42_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDV5877504.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2489..2797
                     /locus_tag="RJE42_002099"
     CDS             2489..2797
                     /locus_tag="RJE42_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDV5877505.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            3395..5209
                     /locus_tag="RJE42_002100"
     CDS             3395..5209
                     /locus_tag="RJE42_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV5877506.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSMISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            5412..8021
                     /gene="clpB"
                     /locus_tag="RJE42_002101"
     CDS             5412..8021
                     /gene="clpB"
                     /locus_tag="RJE42_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV5877507.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIAKLQEKRAQLDENRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         2813 a         1160 c         1457 g         2672 t
ORIGIN      
        1 gaggctgaga cggcacccta ggaaagcgaa gccattcaat acgaagtatt gtataaatag
       61 agaacagcag taagatattt tctaattgaa aatatcttac tgctgtttat tagggattta
      121 tgtcccagtc caacaaaatt ctaacctaga taattaagat tagattgaat cgcatgattc
      181 atttatttag ctttgtaacc aatcatattg attaaatctt tagggtggct atatggtggt
      241 gcataagcca cttcaaactc agttaactca tctacagtta gctggttcat cattgccatc
      301 gatagtacat caatacgttt atctgcacct tcttttccta ctgcagctgc tcttaaaatc
      361 tgatggtttg aagtgtcata atatactctt aagtgtaaag gggaatttcc tgggtaataa
      421 ttcgcgtgtg caccttgagt gacttctacc attttatagt caaattgctt tagttcgttt
      481 ggtttaacgc cgacactcgc aaatgtataa tcaaagaact tcacaatatt gttgcctaag
      541 aagcctttga attcaatagt gtcatttcca gcaatttgtt cggcaacaat acttgctgca
      601 cggtgagcgc cccaagctaa aggaacacta gccggtagat cgacatgtcg ataatgtgat
      661 gttgcaatat cgcctattgc ataaatgttt ggaacatttg tttcaaattt atcgtttacc
      721 ggtatgaaac cttttcgatc aattttgata tttgaacttt cgataaattt tgaattgggg
      781 tgagtaccga caccttcaat aatcatatcg taatgttcaa cttttcctga tttaaatgta
      841 atttcatttc cattgatagt atcaatttcc tcatttaaac ggtatggaat ctcccgctta
      901 tctaattcat caagtatagg ttgattcatg tcggcatcca ttaatttatt tatcttatca
      961 gatcgatgaa ttaaagtagg gtgtaaacca cgttcataaa ggttttcaag aacttctaat
     1021 gaaacatacc ctgcacctac aaccaatact ttatcaactt gatttgcttt gatgaattga
     1081 tcgatagcat cagtgtcttc taaatttcta agtgtaaatg taatatcact ttcaaagcca
     1141 aggctatttg cacttgcacc agggcttaaa atgagtttat cgtatgattc ttcaaattgt
     1201 tcgttggtct ttctatttaa tacagttaca gtttgtcttt catcattgat tgcaataact
     1261 tcatgataag tttttactgt aatttgcttt ctatcataaa atttttcagg tgtatatgct
     1321 aaagcatatt ttctatcttc aacaacttcg ccaatgacat aaggcaatgc acaattagca
     1381 aagctcatat cacgatcttt ttcaaaaata ataatgtcac tttctttatc taaacgtcga
     1441 atttggctgg cacatgtagc accgccagcg actgctccga ctacgactat tttgggcatc
     1501 gtaacttacc tcctattgtt ttaaaatatt ataaaatgat tagttataaa ttaacagtaa
     1561 tatctaatat ttaaattaaa gaaatcattc aaatatcgac caatgccatc ttcattattg
     1621 ttgaatgtaa tattgttcgc tacatcttta agttcttgca aaccattttc catagcaaca
     1681 ccgtgacgtg cgtactcaat catttctata tcattatctt catcaccgaa tgcaataata
     1741 ttatttcggt caatatttaa aaattgtcta acttgttcaa tacctctagc tttattaatt
     1801 ccaagtttta caatttcaat gacagggaat ggtgcgcccc aacgtcgatg ctcaatatga
     1861 tcggcataaa aatgagtaag catatttttg atttcaggta ttttactttc ttcggcttca
     1921 attaaaattg aggtagggga ttctttcaag tggacaagta aattaccagt ttgaattctt
     1981 ggattaccca ttgaaaaacc ttcaaataac cttggatcat gattgttaat gaaaacataa
     2041 tctttcactt ctgcaataat attcgatact tgatattgtt gtaatccttg aataatattt
     2101 tgtgcgatgc ctaaatctaa aatttcatgg caagttttga agtttttatc tttagggtga
     2161 tgtacgtaag cgccattaaa attaacaatt ggtgtcgtta aatttaattc atgataatac
     2221 atttgacttg cacgataagg tctgccagtc gcaatcataa tttggtgtcc acgttgttgt
     2281 aattcattta atacttgttt agtatatgat gaaatttctt tgttatcgtt taataatgtt
     2341 ccgtctaagt ctagacatat taaatgtggt tgcatatctc tctcctcata agttgcatag
     2401 ttcatataga tacatcatag cattttattg gcgttttgtc ggatatttga tatattatga
     2461 ataatatgac attaaagagg tgatatggat ggaagaggca ttgaaagata gtatcttagg
     2521 tgcattagaa atggtaattg accctgaatt aggaattgat atcgttaatt taggtttagt
     2581 atacaaagtg aatgttgatg atgaaggcgt atgtacagtt gatatgactt taacatcaat
     2641 gggatgtcca atgggacctc aaattattga tcaagttaaa acagtattag cagagattcc
     2701 tgaaattcag gatactgaag tgaatatcgt atggagtccg ccttggacaa aagatatgat
     2761 gtcacgttac gctaagattg cacttggtgt gagctaacat aaacgtgcat tgcctgtaga
     2821 aattggaaat ccaattgggg ctccgcttcc aacttgcatt gcttgtagaa tttcttttcg
     2881 aaattctctg tgttggggcc ccgcagtcaa tataaccttg tagagtataa ttcattgtga
     2941 ataagctccc ttcaaagtag acattgaaaa atgaaaattt tgaagggagc cttatttatg
     3001 ttctatgttc tgattttatt ttgaaaattt atatctaact tgtttcaaaa aaatatgaac
     3061 taagacagtg actcgtactt ctgagagtgc acaattaatt ttctagttga atattttctt
     3121 ggaaatttta ataataaatt gaatttatat tacggttata ttacaataca taatacttca
     3181 tagtttttaa gttgccgagt tgcattacaa attggatttc gaaataagaa tcatcattgg
     3241 taattttttt aatataacag tattttagtc aatatgattt tgatgatgtc gtaattcaat
     3301 aatatacata tgcatttcaa tattctttta aacagcataa ctaaatttaa ttaagcatct
     3361 aagaagaaca gttttaaaag ggaagaatgc aaaaatgaac aaaacaaagg gttttacaaa
     3421 atataagaaa atgagatata tgccagggct cgatggtttg agggcaatcg ctgttctagg
     3481 aattattatt taccacttaa ataagcaatg gttgacaggt ggctttttag gtgtggatac
     3541 attttttgtg atctctggtt atttaattac aagcttatta ctcaaagagt atgatgacac
     3601 aggtatcatt aaattgaaaa gcttttggat acgtcgttta aaacgtttat taccagcagt
     3661 catagtttta ttaatggttg taggaacagc aaccttatta ttaaaatcag ataatatcat
     3721 tagggttaaa catgatatta ttgctgcgat attttatgta tcaaactggt ggtatatagc
     3781 aaaagatgtt aattattttg agcaattttc atttatgcca ttaaagcatt tatggtctct
     3841 agcaattgaa gaacagtttt acatattttt cccagttatt ttggttacat tattgttaac
     3901 aattaaaaag cgatacaaaa taggatttat tttttgggga gtatcaatga tttctttagg
     3961 gttaatgatg tttatctaca gtattaatgg ggatcattca cgagtgtatt ttggtacaga
     4021 tactagatta cagacattgt tactgggtgt tattttagct tttttatggc caccgtttaa
     4081 attgaaaaat gatccaccta aagttgtaaa atatgttatt gatagcatag gtagtttatc
     4141 atttatagta cttatattat tatttttcat tattaatgat gagacgaatt ggatatatga
     4201 tggtggtttc tatttaatat ccatattaac attatttatt attgccagtg tcgttcatcc
     4261 atctacatgg atagcgaaga tattttcaaa tccagtgtta gtatttatcg ggaaaaggtc
     4321 ttatagtcta tatttatggc attttgcagt aattagtttc gtacatagtt actatgtaga
     4381 cggacagata cctgtatatg tgtactttat agatataagt ttaacaatta tatttgcaga
     4441 gctatcatat cgctttatag aaactccatt tagaaaagaa ggtattaaag ctttaaattg
     4501 gcgaccttct tatataccac aatttataag aatggcaatt gtagtaacct tgttaattcc
     4561 atttatgttg attttagtag gtgcattcaa taaatatggt aaagacatta taggagaaaa
     4621 agcgaatagc tttgatacca ctattgaaga taattattta atgcggatag caccaattga
     4681 taacattcac attgatggct tagtaagtga gaagaaaaag gaatcttccg acgtatataa
     4741 taatattaaa cctcttttaa tcggtgattc agtaatggtt gatatcggtg agtcatttaa
     4801 gtcatcagtt cctaagtcta gaattgatgg aaaagtaggg cgtcaattgt atcaaacctt
     4861 acctttagtt aaagcgaatt attcacaata taaaaaatca tctgatcaag tcgtattaga
     4921 attaggtaca aatggcgact ttactgtcaa acagctcgac gatttactta atcaatttgg
     4981 aaaagccaag atttatttag ttaatacacg tgttccaaga atttatgagg caaatgtaaa
     5041 tcgattatta gctgacgcgg cgaaacgaaa gtccaatgtc acattaattg attggtataa
     5101 acgatcacaa ggacatagtg aatattttgc accagacggt gtacatttag agtacaaagg
     5161 agtcctagct ttaaaagatg aaatattaaa agcacttaaa aagaaataac atatgtttaa
     5221 taagtctagt taatgtgtaa cgtaacatta gctagatttt tttaattcaa aaaattattt
     5281 acaaatatta ggaaatttaa gtgtaaaaga gttgataaat gattatattg ggactataat
     5341 ataattaagg tcaaagaaag tcaaagtcaa aagtgcaact tgaccaactc tacaaattag
     5401 aggtgaaatt aatggatata aataaaatga catatgctgt tcaaagtgct ttacaacaag
     5461 cagttgaact gagtcagcaa cataaattac aaaatataga aattgaggca attttaagcg
     5521 ctgccttaaa tgaaagtgaa agcttatata aaagtatttt agaacgagca aatattgagg
     5581 tagatcaatt aaacaaagct tatgaagaca aactaaacac gtatgcatct gtagaaggtg
     5641 acaatataca atatggtcaa tatattagcc aacaagcaaa ccaattgata actaaggctg
     5701 aatcatacat gaaagaatat gaagatgaat atatttcaat ggagcatatt ttacgttcgg
     5761 caatggacat tgatcaaaca acaaaacatt atataaataa taaagtagaa gttatcaaag
     5821 aaattattaa aaaagtaaga gggggaaatc acgtgacatc acaaaatcct gaagttaatt
     5881 acgaagcatt agctaaatat ggccgcgact tagtagaaga agttagacaa ggtaaaatgg
     5941 atcctgttat aggaagagat gaagaaattc gaaatacgat tcgtatttta agtcgtaaaa
     6001 ctaaaaacaa ccctgtgctc attggtgaac caggtgttgg taaaactgca attgttgaag
     6061 gattagcgca acgtatagtt aagaaagatg tgccagaatc attattagat aaaactgttt
     6121 ttgagttaga tttaagcgca ttagtagcgg gcgctaaata tcgtggtgaa tttgaagaga
     6181 gattaaaagc agtcctaaaa gaagttaaag agtctgatgg tagaattata ttatttattg
     6241 atgaaatcca tatgcttgta ggtgctggta aaacagatgg tgccatggat gcaggcaaca
     6301 tgctaaaacc aatgttagca cgaggagagt tacattgtat tggtgcaaca actttaaatg
     6361 aatatcgaga atatattgaa aaagattcgg cattagagcg tcgtttccaa aaagtagcag
     6421 ttagtgagcc tgatgttgaa gatacaattt caattttacg tggtttaaaa gaacgatatg
     6481 aagtgtatca tggtgtgcgt attcaagata gagccttagt tgctgccgct gaattgtctg
     6541 atcgttacat cactgatcgt tttttaccag ataaagcgat tgatttagtt gaccaagcat
     6601 gtgcaacaat tcgtacggaa atgggatcaa atccaactga attggatcaa gttaatagac
     6661 gtgtcatgca attagaaatt gaagaaagcg cacttaaaaa tgaatctgac aatgcgagca
     6721 aacagagatt acaagaacta caagaagagc ttgccaatga aaaagagaaa caagcagcac
     6781 ttcaatctcg tgtagaatca gaaaaagaaa aaatagcaaa gttacaagaa aaacgtgcgc
     6841 aattagatga aaatagacaa gcgttggaag atgcacaaac aaataacaat ttagaaaaag
     6901 ctgctgaact acaatatgga acaattcctc aattggaaaa agaacttaga gaattagagg
     6961 ataatttcca agatgagcaa ggtgaagata cagatcgaat gattcgtgaa gttgtaacag
     7021 acgaagaaat tggcgatatt gtcagccaat ggacaggcat accagtttca aaattagttg
     7081 aaacagaacg tgaaaaatta cttcacttaa gtgatatctt gcataaacgt gttgtaggtc
     7141 aagataaagc ggttgacctg gtttcagatg cagtagttag agctagagca ggtattaaag
     7201 atccaaacag acctattggt agtttcttat tcctaggtcc aactggggta ggtaaaactg
     7261 aattagctaa atcattagct gcatcattat ttgattctga aaaacatatg attcgtattg
     7321 atatgagtga atatatggaa aaacatgcag tatcaagatt gattggggca cctccaggat
     7381 atattggaca tgatgaaggg ggtcaattaa ctgaagcggt tcgtcgtaat ccatactcag
     7441 ttattttatt agatgaggtt gaaaaagcgc atactgacgt ctttaatgta ttattgcaaa
     7501 ttttagatga aggccgttta actgattcta aaggacgtag cgttgatttt aaaaatacta
     7561 ttattattat gacaagtaat attggatctc aagttttact agaaaacgta aaagagactg
     7621 gtgaaattac agaatcaaca gaaaaagctg ttatgacaag tttaaatgca tatttcaaac
     7681 cagaaatttt gaatcgtatg gatgacatcg tattatttaa acctttatct attgatgaca
     7741 tgagtatgat tgtagacaaa atattaacgc aattaaatat aagattacta gaacaacgaa
     7801 tctcaattga agtttctgat gatgctaaag cttggctagg tcaagaggct tatgaacctc
     7861 aatacggtgc aagaccatta aaacgttttg tacaacgcca aattgaaaca ccattagcac
     7921 gtatgatgat taaagaggga ttcccagaag gtacaactat taaagttaat ttaaattcag
     7981 acaataactt aacgtttaat gttgaaaaaa ttcatgaata aaattgaacc aagaatgtga
     8041 tgattaatcg cattcttggt tttttagatt tgaaatttat tataattgtt cattggcaat
     8101 at
//
LOCUS       DAPIDM010000116         8035 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000116 DAPIDM010000000
VERSION     DAPIDM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.116"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..205)
                     /locus_tag="RJE42_002102"
     CDS             complement(<1..205)
                     /locus_tag="RJE42_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877508.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDA"
     gene            430..1011
                     /locus_tag="RJE42_002103"
     CDS             430..1011
                     /locus_tag="RJE42_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV5877509.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1084..1701)
                     /locus_tag="RJE42_002104"
     CDS             complement(1084..1701)
                     /locus_tag="RJE42_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDV5877510.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYVIGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKIVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLIF"
     gene            complement(1850..2344)
                     /locus_tag="RJE42_002105"
     CDS             complement(1850..2344)
                     /locus_tag="RJE42_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877511.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENEALIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(2743..3165)
                     /locus_tag="RJE42_002106"
     CDS             complement(2743..3165)
                     /locus_tag="RJE42_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDV5877512.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRAIDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDPDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLIVNVVD"
     gene            3313..4029
                     /locus_tag="RJE42_002107"
     CDS             3313..4029
                     /locus_tag="RJE42_002107"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV5877513.1"
                     /translation="MTHVEVVATIAPQLYIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVIISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQSMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            4112..4651
                     /locus_tag="RJE42_002108"
     CDS             4112..4651
                     /locus_tag="RJE42_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDV5877514.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEVGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(4801..5121)
                     /locus_tag="RJE42_002109"
     CDS             complement(4801..5121)
                     /locus_tag="RJE42_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV5877515.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5265..5621
                     /locus_tag="RJE42_002110"
     CDS             5265..5621
                     /locus_tag="RJE42_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDV5877516.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            5779..6159
                     /gene="gcvH"
                     /locus_tag="RJE42_002111"
     CDS             5779..6159
                     /gene="gcvH"
                     /locus_tag="RJE42_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV5877517.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEVSDESQLEALLSAEKYAEMIGE"
     gene            6339..7217
                     /locus_tag="RJE42_002112"
     CDS             6339..7217
                     /locus_tag="RJE42_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDV5877518.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPILPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPISDLSLKDIQAHLKSLNADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQGLKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            7919..>8035
                     /locus_tag="RJE42_002113"
     CDS             7919..>8035
                     /locus_tag="RJE42_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topiosmerase"
                     /protein_id="HDV5877519.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSID"
BASE COUNT         2837 a         1128 c         1382 g         2688 t
ORIGIN      
        1 gtgcatccca aatatctttt gttttaaagt ttatttcatc atttcttatc gaaaatggtg
       61 taataatgtc tttatctaac caagtgttta taagttcatt tggtacacca tctaacaaca
      121 tttcactttt actaattata aaacattccc agtcaagtga aacattttgt ggattcacat
      181 aattacattg attatgatta tccataaaca ctcactcctt taaaattctg tactcttcat
      241 tgcgttttac cccgtcacat tatcttttaa actaaaatca tcattactta tgaaaaaaat
      301 gttcatcaaa agcaaaggtt ttcgctaccg aaaaagttta aataatggtt taatatattt
      361 ggtactcatt ttaataaaaa gagaatacat tttgagctat caatactttt tattgaagag
      421 gtgttattgt tggctaaaac gttatattta atgcgccacg gacaaacttt gtttaatttt
      481 aagggactaa ttcagggatt tggagattcg ccgctaacag aacttggaat tgctcaagct
      541 caaaaggcac gtagttatta tgaaactaag gggataaact tcgatttata tgcatcatca
      601 acgcaagaac gcgcaagtga cacacttgaa aatgttgcac ctaatcaatc gtatcaacgt
      661 tttaagggac tgaaggaatg gcattttgga ttatttgaag gtgagtcagt ctatctattt
      721 gataatctat acaagcctga ggacttattc ggagatcgaa ttgttccttt caaaggagag
      781 gcaaggcaac aagttgaaga tcgcattgtg aaaactttac atgacattat gtctcaaaca
      841 aagaataatg cattagtcgt gagtcatgga acaataatgg gagtattttt aagatattgc
      901 cttaaactag atgaagcatt aaagcataat attggtaatt gtaatatctt gaaatttgaa
      961 tatgacaatg gaacatttaa atttgttgag ttaattgatc caaatttata ataaataaag
     1021 cgttatacat agacacaagt atgtcttttg tataacgctt ttaaaatact actatataac
     1081 gctttagaat attaattgaa taagtttttg caatatcatc agagcaacaa taataattat
     1141 tatgcttgat attttattga ttattgttaa taattttcct gttttatcaa ttgatccaac
     1201 tatttttcct aaaattgcga gtaaaaagaa ccataaccat gaaacgctaa tacaagcgat
     1261 tgtgaacgca attttattgc tgccactata taatgcagca ctactaccaa ttacgccaat
     1321 tgtatctaaa atagcatgtg gattgagtaa tgaaactgat aaagcaaaac ttacttgttt
     1381 cattggagac ataatttgag cttctccatc tgttgagggt ttatcatgcc aaatggtcca
     1441 agccatatac atcaagaaaa ttaaaccaat tacataaata attgcttgaa gtacaggtaa
     1501 agacataata atgatagata ctcctaccac tgcaataata ataagtaagc tgtctgacaa
     1561 cccggctgta attattgcag gcaatacata tctatatttt ggttgattag ctccttggtt
     1621 aaaaataaat acattttgtg cacctaaagg taaaattaga ccaatcgcta agataaatcc
     1681 atgaataatt gcggttacca ttaagttttg acacccctaa aacatagaat attctgaaaa
     1741 aacttattga tatcatacca aataaaaaac aactactcaa tagtagagta gttgtatgaa
     1801 aaatagattg atttgaattt atgagaaatg tagaatatat aaaactgtat tacttattaa
     1861 tcaaatcata aaaaagccaa tgttcatcga aataggtatt tccgatttta attgcatttt
     1921 tctcaaaacc aagaatgtcg aatcctagtg cactatagaa aactttagca cctatgttgt
     1981 ttgacacaat tgatgtaagt aaagattcgt aattgttctg ccgtgcataa ttaataatga
     2041 aattaatgag ctcgctatta atcgatttat cgttattagt aacaaagttg tatttaatta
     2101 atgatttatg ttcttttcca acgtatcgaa tttgttctaa ggcagctgtt gcgataagtg
     2161 cttcgttttc ataacagcca aatacaatac atttcggtga accttgattt aaaatgtcgt
     2221 gtattaaggt gtcagtcatt gtttgactat aatgtgaatc ttgagtgaat tcttcattga
     2281 ctgtcgaaac aagtctctta tactcattaa aatcattatt cgttaatgtt ttaatttcaa
     2341 tcattactga cctccttaat cattattaag aaagattttg tactaaaaaa atttcttaat
     2401 tacatattaa aatgtttatt aaaattttgc aatcattgat ttatatgcac attcttgtaa
     2461 atggattatc aatttaatta tatttatttt taagttcagg gaattatgga attggttatt
     2521 ggtcgacact ggacttttat ttgtagacgg tatgcgaaga agtcaagcca gtattggaac
     2581 cggtcacatt tatgatgact aagggacaat cattttcagt taaatattgc tagaaatttc
     2641 aatcgcgatt agaaaaaata accaaccaaa aatttttggt tggcaaattt gaattttatg
     2701 tgaattttat taataacgtt gaataatcac tttaagttaa tgttaatcta caacatttac
     2761 aattaaatcg acattaatat ttccttgagt cgcttttgaa tatggacaaa attcatgagc
     2821 catttgtaaa tatttttcag cttcttcttg agatataaca tttttaattg tcgcatcaat
     2881 tgaaacactt aatttaggac tttctgagtc tggatcatct tctagtctca ctgttagtgt
     2941 tacttctgga tgagcatcac gcactttgtt ttgctttaaa attaggtcga aggcaccgtt
     3001 gaagcaagat gcataacctg ctgcaaataa ttgttcgggg ttagtagctt taccatctgc
     3061 ttgagcaggc ggaacgatat caatatctat tgctcgatca tcagtataaa catgtccttt
     3121 acgtccgcca acatttgtcg ctttagtttc ataatgtatt gccattctta taacctccta
     3181 atttaaaata catttatgct ttacccattc gaaaatacct taatcatatt catttatagc
     3241 attgtttgat tgaaggataa aaagttgttg tttacaataa aaataatgag tatctgaaat
     3301 gagggattta ctatgacaca tgtggaagta gtagcgacta tcgcgccaca attatatatc
     3361 gaagaaactt taattcaaaa aattaatcat cgtattgatg caatagacgt attagaatta
     3421 cgaattgatc aaattgaaaa tgtcacagtt gatcaagtgg cagaaatgat tacaaagctg
     3481 aaggttatgc aagattcatt taaattatta gttacgtatc gtacaaagtt acaaggtggc
     3541 tatgggcaat ttacaaatga ctcgtatctt aatttaatat cggacttagc aaatatcaat
     3601 ggcatagata tgattgatat agaatggcaa gcagatattg acattgaaaa acatcaacga
     3661 atcattacac atttgcaaca gtataataaa gaggtgatta tatcacatca taatttcgaa
     3721 agtacgcctc cattagatga attgcaattt atatttttta aaatgcaaaa gttcaaccca
     3781 gaatacgtta aattagcagt aatgccacat aataaaaatg atgtgttaaa tttattgcag
     3841 tcaatgtcta cattttcaga tactatggac tgcaaagttg ttggtatttc aatgtctaaa
     3901 cttggactaa taagtagaac ggctcaaggc gtttttggtg gtgcgttgac ttatggttgt
     3961 atcggagaac cacaagctcc aggacagatt gatgttactg atttaaaagc acaagtgact
     4021 ttatactaat aaaatgaatt aggttgcttt tgtgtaagtg ctgtcttata attgagaagt
     4081 attatcattg ttcgtggagg agcgatttta gatggaatta caacaagcaa tagctaatag
     4141 aagaagtgtg aaaaaattta aaagagatat gcacatagat gacgcattgc tatatcaagc
     4201 aattgagaaa gctgctgatg ctccaaatca cggaatgagg gaaccatgga gagttgtgca
     4261 tgttccgaaa gacagattag gagatatgag taaggatatt tctaaatttg catttcctaa
     4321 tgaattagat aagcaacaat gtcattatga tgcagtaacg aaactaggtg gcatgttatt
     4381 gcttatttta aaaacagatc caagacaacg tcaaaatgat gaaaactact ttgcatttgg
     4441 tgcatatgca caaaatctta tgttgttact ttatgaagtg ggaataggta catgttggaa
     4501 atcgccatta tatatctatg atcctaaagt aagaaaaaca cttggtataa agaaagacga
     4561 agttcttgct ggattcttat atttaacgga tttagaagaa gatatgccta aagcaccacg
     4621 taaaaataga aacttaatta cattatatta atatgtataa ttatagaaac attaataaaa
     4681 gctgagtcat gaattgaagg acatctatcg agttagagat ttaatctaac ttactagagt
     4741 cggtacaatc acagtctcag ctttttattg tgcagtatat acacatttgt attttagtat
     4801 ttatttaaaa gtttctgcta aaaatgattc aacttgttca ggtgacttag catttgctga
     4861 atgaaggtgt gcaattttgt cgccgttttt aaatactagc aagctaggga tacccataac
     4921 ttcattttca acaactacat cttctaattc atcacgatta acagtatacc attggtaatc
     4981 attatattgt tctacgattg ggtcaatcca taaatccata gcacgacagt ctgggcacca
     5041 tcctgcctca aatttaacaa ttacaggtgt atcgctatta attacagatt taaatgattc
     5101 attacttttg attgcttgca ttgtaacaac tcctctagat agtttaataa tttttattat
     5161 agctaaattt atatcataat aaaaaatttt agcttcaaaa tgaaagcctt ttgctatgga
     5221 aaatgatata tttattttaa atacataaag gaggttgcag acgtatgatt aaattttacc
     5281 aatataagaa ttgtacaact tgtaaaaagg cagcaaagtt tttagatgaa tatggtgtaa
     5341 gttatgaacc aattgatatc gttcaacata cacctacaat aaatgaattt aaaacaataa
     5401 ttgcaaatac aggcgtagaa attaataaat tgtttaatac acacggcgcg aaatatcgtg
     5461 agcttgattt gaaaaataaa ttacaaactt tatcagatga tgaaaagtta gagttgttat
     5521 catctgatgg tatgttagta aagcgtcctc tagcagtaat gggcgataag ataacattag
     5581 gatttaaaga agatcaatat aaagagactt ggttagcgta agcataatgt aaacgtttac
     5641 taaatatctc gatatttaga ttcattacat gtaaaatgaa ataagctata caattgttaa
     5701 tttttataaa tatagttgaa tagcatctag ccttatggca tcattaatga tgtaaagatt
     5761 aattaggagg ggattctctt ggcagtacca aatgaattga aatattcaaa agagcatgaa
     5821 tgggttaaag ttgaaggtaa tgtagcaaca attggaatca cagaatacgc acaaagcgag
     5881 ttaggtgata ttgtttttgt tgaattacca gaaacagatg atgaaattaa tgaaggggat
     5941 acgtttggta gcgtagaatc agttaaaact gtatcagaat tatatgcacc aatctctggt
     6001 aaagtagttg aagtcaacga agaactagaa gatagtcccg aatttgtaaa tgaatctcca
     6061 tacgaaaaag catggatggt aaaagtagaa gttagtgatg aaagtcagct tgaagcatta
     6121 ttatcagctg aaaaatacgc agaaatgatt ggtgaataat caccgtgtaa ctccttaatc
     6181 taagattgag gagtttattt ttagtctgag taagtaaata aatatgaaag aaaatttatt
     6241 aggtactatt atttggagta ttgctacatt ttattattca agaatgatga aaataatgaa
     6301 tttagctatt ttaaaaataa aaattggggg aagttaatat gctaaacatt caagacgtta
     6361 gtcatctttc taaaaaggag caaaaagcat ataaccgttt cgtagaatct gtagaaaacg
     6421 gtaatttacc aatactacca tgtattgaaa tggatctaaa agagatgcaa gaagaaacat
     6481 taaaccagag taagattggt ggaatgccat ttttaaaatc ttttaaagat ataccattag
     6541 atgaaaataa tgtaccaatg gtattgctag cacagattaa tttggatgat cttccagaac
     6601 aacaagaatt atttcctgta aatgaaggga tattgcagtt ttggattagt tcagaagatc
     6661 aaatgtatgg tatgtctgaa aatttaaagg gaaacaatat aaactcaagg cttgtttata
     6721 taaaagagcc aatttcagat ttatcactca aagatattca agcgcatttg aagtcattaa
     6781 atgctgataa tgaggatatc ccgttcagtg gagcattttc tatagaattt agattgtcga
     6841 aacaaactat tacatgtact gattataagt acgatgagga cgtgcttgca ttgtggaata
     6901 aagtcaatcc atccttcgcg ctaaaatcaa tgtttggtgg atatgatgaa ttaatggaac
     6961 ctgtgtgtaa cacatttact gctaaggaac catttaatca acttggtggt tatccatatt
     7021 ttgaccaaat agatccaaga acgaacgatc aaggactgaa aatgtatgat agagtcttac
     7081 tgcaaattga ttctacaaga gatggtaatt cttcgataat atggggtgat ttaggtattg
     7141 ccaatatctt agtaaaatct actgaccttg aggctatgaa gtttgatgat tacatgtatt
     7201 catgggattg cagctaagag aatcataaaa atatattact ttaataagat gtagtattta
     7261 taggtcaatc tagggagtgg gacagaaatg ataaagagcc actaatgatt tattatgtag
     7321 tggttcttac acattagcca cagctaatgt gtacttaaaa ataggaatac atgagtaaaa
     7381 ctcatgcata agaaatacta atttcaatag aaaaagtatt tctttatcgt cgtcccaccc
     7441 caacttgcat tgtctgtaga aattgggaat ccaatttctc tgtgttgggg ccccgccaac
     7501 ttgcattgtc tgtagaaatt aggaatccaa tttctctgtg ttggggcccc gccaacttgc
     7561 attgtctgta gaatttcttt tcgaaattct ctatgttggg gcccacccca acttgcacat
     7621 tattgtaagc tgacttaccg tcagcttcta tgttggggcc ccgctataat tgaaaaatgc
     7681 ttgttatagg tgtattttct tttggttaac tattggtaat atatcattgt agattttagg
     7741 atgttgattt atggtttagc ctttgtctgt ttatgtaaaa tgtcccagct agtcaaacat
     7801 tgagtttgta attattatat taagctagtt taaatttttt gtgatttatg ttttattcat
     7861 accaattttg tgaaatgata cagtgacaga tgtttattga ttttaaaggg tgatgatgat
     7921 ggctattgta aataaagtga taattgttga aggaaaatct gataaaaaaa gggtgcaaca
     7981 ggttattgca gaaccagtca atattatttg tactcatgga acaatgagta tagat
//
LOCUS       DAPIDM010000117         7894 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000117 DAPIDM010000000
VERSION     DAPIDM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.117"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1254)
                     /locus_tag="RJE42_002114"
     CDS             complement(<1..1254)
                     /locus_tag="RJE42_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDV5877520.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHNQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGH"
     gene            1402..1587
                     /locus_tag="RJE42_002115"
     CDS             1402..1587
                     /locus_tag="RJE42_002115"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDV5877521.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(1675..2658)
                     /locus_tag="RJE42_002116"
     CDS             complement(1675..2658)
                     /locus_tag="RJE42_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDV5877522.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            2795..3304
                     /gene="msrA"
                     /locus_tag="RJE42_002117"
     CDS             2795..3304
                     /gene="msrA"
                     /locus_tag="RJE42_002117"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV5877523.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(3507..6029)
                     /gene="mprF"
                     /locus_tag="RJE42_002118"
     CDS             complement(3507..6029)
                     /gene="mprF"
                     /locus_tag="RJE42_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDV5877524.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILN
                     KITWVRWVLYAVSLFLPLFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDVHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYLLLAIHTSACLLLLLNVVGIYK
                     QSRRAIIYAMISIILIIVATLFTYASYILITWLVIIFALLIVAFRRARRLKRPIRMRN
                     LVAMLLFSIFILYINHIFIAGTFYALDVYTIEMHTSVLKYYFWITILIIAIIVGAIAW
                     LFDYQFSKVRISSNIEECEEIIDQYGGNYLSHLIYSGDKQFFTNENKNAFLMYRYKAS
                     SLVVLGDPIGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIVEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNENNEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(6509..7717)
                     /locus_tag="RJE42_002119"
     CDS             complement(6509..7717)
                     /locus_tag="RJE42_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV5877525.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
BASE COUNT         2826 a         1407 c         1070 g         2591 t
ORIGIN      
        1 atgtccagcc attaaaccta atctaccatg ttttgtacct gcttctgtaa acgatactgc
       61 tttggacaca atacctttgc cgtatttcaa atgtaattgg tctatcgttt tagctagaca
      121 ttcgtcacgt ttgcgttggt attcatcttc aaacagactt aactgtcgct catcctcatt
      181 aataaattga ctcaaagata tacttagcgt acgatatagt gcttgtttat tacataattt
      241 atctgcgaaa tgcattacta ctttataaat atctttttct aggtttgttg gatctttcaa
      301 agtatattgc ttatgtacac caccttcatc actatagcca aaggcaaaat gtatcgttct
      361 tgccactttt tttcttgctc gaactctgct agcaacatct tcaattaact cttgcattac
      421 tacttttgct tcgtcaaaat gataatctct cattaatatt tgacttttgc atatcgatgg
      481 attgctaatc ttatgctttt cacgtacttt actctgatct atcccattcg catgtagatg
      541 catatcaaca cctaaaatac cgaactcttt ttttaaaaat ttatatggat attttgctaa
      601 atctcctata gtaaaaatcc ctcttttatt caatttggct tctgttcgac gattaatacc
      661 ccaaaaatct ctcaagggct gaattggcca taattttgtt ggtacatctt gatatcgcca
      721 ttcagctata ccattttgat tatgcttagc ttcaacatcc atagcaattt tacttaataa
      781 catattagaa ccaatgccca cagtacaata aatgcctgtt tcttcataaa tttcacgttt
      841 aagtctttcg caaaatgcat gtactgtaga actaaatcta tgatagctat cagtaacatc
      901 cataaaaaat tcgtcaatac tatattggtg taaatcttca ggtggaatat aacgcaatgc
      961 aatcttagaa atagcaactg aaacattaag atatttacgc atacttggat tgataatgta
     1021 aatatcattt ctatgtggta tttcaaacaa tcgcgatcct gtcttgatgc ctaattcttt
     1081 taatttaggt gtcgcagcca atactacaga accctgacgc ttagtatctg caacaacagc
     1141 taactttgtt tctaatggat ctagcccctt ttcaatacaa gaaacactcg caaaaaaact
     1201 tttttgatca atacatagaa catccctatc ttctaataaa tgataattat acactgttat
     1261 tcctccctta cttgaattcc attatatacg aacacttgtt ctatttcaac cattttagaa
     1321 caaatgttct attattttac aaatgaattt ttaagtatta catgatatac tcaaattaat
     1381 aatgattagg aggtttggat gatgccaatc gtcaatgtaa aattattaga aggtcgttcg
     1441 gatgaacaat taaaaaattt agttagcgaa gtaactgacg ccgtagaaaa aacaacgggg
     1501 gcaaatagac aagcaattca cgttgttata gaagaaatga aaccaaacca ttatggtgtg
     1561 gctggcgtaa gaaagtcaga tcaataattc ttcataagat gcatgccaat taattctttg
     1621 aaaacgaaca aggcgacttc tatctgagta tgatagaaat cgccttgttt atttttaatc
     1681 ttcatctaaa aagtctttaa tagcttgttt atttgtgttt ttattaatct gtaatgcact
     1741 accatcagta tttgtattga catcttcgta tgagttcttg attggcacag tcaatgactt
     1801 aacatctttt tcacctcgga taccaaaact caaacctgtt tggaaaatcc ctgaatcagg
     1861 aatgtttgta ttcacatagc ctcttaaaat acctgcaact tttggtaatt taacaactgt
     1921 tctaaaatta accatttctt ttttcaatgt ttgcattact tgttgctgac gtcgcacgcg
     1981 tccgaagtca ccttcagggt cgtgacggaa tcttgcataa ccaagtaatt ctttgccatt
     2041 caacctatgg ttaccctttt tcaaagatac accaatattt ttcgacatat ctttttcgac
     2101 attaattggt acaccttctg gcattaattc atcaatcatt ttctcaaatc cagtaaaatc
     2161 aactactgca taatattcag gattaattcc taaattttta tcaagtgttt ttctaagtag
     2221 ctctggacca cctaaagcgt atgctgaatt aattttgtgt tttccatatc ctggaatatc
     2281 tgcataaatg tcacgcatga cagacatcat tttcatcttt ttattgataa agtcatattg
     2341 aacaaccatg atagaatctg ttcttgattg tccaccttgt gctttatctg caccgagtac
     2401 aagaatagaa attttaccat cattttttac tggtccatta aattgatgta ctttaacatc
     2461 tttcgcatgt ttcttggcat attctacacc gctattgtaa ctatgtacaa tatatacaac
     2521 taatgcgata agtaaaatta caacaatcag aagaatgata ggtaatttcc taattttctt
     2581 cttctttttt cgcttaggcg ccgaagtgcg atgttcactt tgacgtctat attcgttgtc
     2641 attagtttct ttatccatat ttacctacct tatatcttca aaaatagatt tttgtgtact
     2701 ataataatca catattaacg ttatataaaa acaattcaaa aagcaagtga tttcttaata
     2761 aatcgtacta aggtctaatg aaaggatgtc aaaaatgaat attaatacag cttattttgc
     2821 cggaggttgc ttttggtgta tgacgaaacc atttgacacc tttgacggca tagaaaaagt
     2881 aacttctgga tatatgggcg gacatattga aaatcctact tacgaacaag taaaatcagg
     2941 tacgagcggt catttagaaa ctgttgaaat tcaatatgat gttgcattat tctcatacaa
     3001 taagttatta gaaatatttt tctcagtcat tgacccatta gatacaggtg gtcaatatca
     3061 agaccgtggt cctcaatatc aaacagctat tttctacact aatgatcatc aaaaagaact
     3121 cgctgagact tatatcgagc agcttaaaaa tacgattaat gctgacaagg caattgcaac
     3181 aaaaatatta ccagcgtcac aattttacaa agccgaagac tatcaccaag atttttataa
     3241 gaaaaatcca gagcgctatg cagaagaaca aaaaatacgc caagaataca aaaataagca
     3301 ataacattta tacttgatga acctgattgg actgataata aaagttaggc gacttaactt
     3361 aaactcattt caatattaaa aaacggtaca gataggaaac ttaaatattc ttatctgtac
     3421 cgttttttat aaatcattct ttatagaaac caaaaaatcc aagcgcttca ggcataactg
     3481 tatacctctt agcacttgga ttttaattat ttgtgacgta ttacacgcat tactttagaa
     3541 agtgattccc aaagcgaatt atctttacga taaactaaaa agcgtggttc ccaattcgga
     3601 ttatatttag atttataacg acgtaatcct tggaaacgat atagaccgtt gaaatgttca
     3661 aagacacggc ctgcaagtcg ttctcttaaa tatgaataat gcaattgacc aacatttgat
     3721 aacgtcgcca tacccatatt gaatttcgta tagccttgct ctttactcca aagtaacatg
     3781 tgcaagtata atccatccat taacggtaaa tctaactctg gcaaccatct aattaaatcg
     3841 actgaaatag catcattaaa gtatgttggc attaaactac aaaatgcaat tacttcatta
     3901 ttttcattgc gcatcacacc aattggcgct ttagataagt attcttcatt aaattgacca
     3961 acagagaaat gcatttcctg acgattatct agccacaaat cacttacatg ttgaagttca
     4021 tttataaatt ccgttgaaaa cggtggttca acaatttcga atgaaatatt aagttcatcg
     4081 aatttgttta aagtcgctct aaatccacgg cgttttttac ctgaagttga aaattgcgtt
     4141 aaatcaataa ttgcttcttc acctaatttg aaaaattggt tgccgaaatt atgatataaa
     4201 ggcatgtgtt gatctgtaac ttgatagaat ataacatcat agcctaaata ctcagcgtaa
     4261 ttatagaatg cttctaacaa ttcatcaaag gcattttcat caccgattgg atcgccaaga
     4321 actactaatg aacttgcttt atagcgatac attaaaaatg catttttatt ttcattagta
     4381 aagaactgtt tatcaccact ataaattaaa tgacttaaat aattcccacc atattgatca
     4441 ataatctctt cgcactcttc aatattagaa gatatacgca ctttactaaa ttgataatcg
     4501 aataaccacg caatcgcacc aacaataatt gcgataatta aaatggtaat ccagaaataa
     4561 tatttaagta cagacgtatg catttcgatt gtataaacat ctaaagcata aaatgttcca
     4621 gctataaata tatgattgat gtagagtata aatatgctga acaaaagcat tgcgactaga
     4681 tttctcattc gaattggacg ttttaaccta cgtgctctac ggaaagcaac aataagtaaa
     4741 gcgaagataa taactaacca agttattaaa atgtatgatg cataagtgaa taacgtcgcg
     4801 acaataatta agataatcga aatcattgca taaataatgg cacgtctact ttgtttataa
     4861 atacctacaa cattaagtaa aagtaataaa caggcacttg tatgtattgc caataaaaga
     4921 tagtacgtta aatgattgcc atcatataaa gcatcgtata caatcgtcaa attatttaca
     4981 aagaaaatca tacttgtaaa gaatactaaa attgccaatg ataatgatgg tattttagca
     5041 ataatatctt tttgatacga cattaaaaac gatgtcacat ctttagccgg aatgaaatat
     5101 ttagatcctt caatatattt cttagctgaa gtaccaaact caaatgatga aagaattaat
     5161 gcaataatca ctggtacgaa atagtacgca aagcgataaa gtagtagcat taataatact
     5221 ttttcttcag gtacacctaa cgttttaaag cctaataaaa caactaagtc gaaggcacca
     5281 aaaccacctg gaataaagct gactaatcca gataatgcag caataatgaa tattgcaata
     5341 aatgacataa atgagacatg gacgtcaact atgacaccac agaagtataa tacaactgca
     5401 gcggctaacc attccacaca tgacactaat gtacaataca agcctacata acgattgttt
     5461 ttatcgggcg gtctaaccat tgaatatata ataaataatg gtaagaacaa cgacacagca
     5521 taaagtaccc atctcaccca cgtaatttta tttaaaatta atgatgcatc aaaaacatgg
     5581 aatacgatta acaatgataa caaacttaaa cctgtcaaca ttgaaatcag tattaatgaa
     5641 ataaagtgaa ccaatttctt tttatcatgc gtatagtttt tgtaaaccat tgctcttaca
     5701 ccggcaccaa taaagccacc aaatcctaca atcgcattta atgcattaat gatataactt
     5761 actcttaaaa cttttcctaa tgaaatatcc atttttaaag ctctagataa aatcacatca
     5821 tacattgaga gaataacaag cgatgcccca ccaccaataa ataataagac aagggacata
     5881 cggttgattt tactaaattc aaccaaagta tctttaaagt taatcccaga taactctcga
     5941 tacaacgtga ttactacaaa aataaataaa gctgtagcaa acgtaatttt taagattgaa
     6001 aatattttgt ttttaacttc ctgattcatt ttttcacatc aattctaatt atttctgtta
     6061 taaatcaaaa tatatcatat aaaccataaa aatagcatat catttacctt tgatttatat
     6121 aacacttttg tcatattgac atctgtatta agaagtaaat agaaaatatt tctatctcaa
     6181 acagaacaat atggttatct atactgattc atatttaaca taaagaaaat gatgaatcaa
     6241 tacttatgaa aaatattatt aattaacagc tgattatgag tgcttcttaa tatatcaaaa
     6301 taccacttgt ttttattttc gacataataa agaagtacta atcaagtcac aaatactcgt
     6361 ttcattatgc atgtgcttta taaatggcta aactttcata aaaatttata agctaaacat
     6421 gtgctgataa tcataaattt gaacacccaa cattataagc accataaaaa tatcaacaaa
     6481 cgtatagtct attggtattt ttaattactt attcaactat tttattactt tctttaatat
     6541 catattcacc tacatcatta ccatagaaac gattgtatcg acgtttaaat aattggaata
     6601 aatgagtaac taatactttt aaaatagcat atcccggaat acctaaaata acgcctacaa
     6661 taccaagcaa tttgcctgca cacagtaaaa tgaaaatgat tgtaagtgga tgaatcttaa
     6721 gtgttttacc catgatattt ggtgaaatga agtgcccttc aacaaattgt actaaagtcc
     6781 atactactgc taattttaag agcatccacg gcgaagttat agcagcaatt acaatagctg
     6841 gtgaaatcgc tattgtaggc cctaaatatg gtacaacgct tgtaactgca gcaatactag
     6901 ctaatactaa gctatatttc aacccgataa ccgaataacc gataaacaac agtataccga
     6961 tacagaatga aacgataatt tgtccttgaa tgtatgaacc aacttgaaca ctcatttttt
     7021 caagtagatc atgaaaatct tttcggaatt tcggtggcat aatattcgtt gaaaattctt
     7081 tgaagtgatg tccatcttta agcatgaaga atagtacaaa tggtgttgtg acaatcacaa
     7141 cgccaatatt agcaatcgtt tctgcaaacg ttgcaatttt agacccaaaa ccatctgtaa
     7201 attcacttaa catagatggt attttcttag gtaaagaatt taaccaatca ttaatttgac
     7261 tataatacga agagaaaaac gtattatttg ctattttatc aatagaatta attaattttt
     7321 ctagatattg cggtgaattt ttaactaaac tatctacttg cgaaccaata ataggtatca
     7381 ataaattaac aattaacgta ataacaccta ctacagctag ataaataata gaaatacctg
     7441 caactcttgg tattctataa cgctccatca tatttacgat tgggttaaat agatagaata
     7501 gaattaaaga gacgatgata ggtgctgcta tcgtcttaaa aacgatgata aaaggatcaa
     7561 aaacgtatga tactttttcg aaaataaaaa taacaatacc caacaatact agtgctatta
     7621 atgagaagac taaatcattc ccaccaacaa acttcatata ccgtgattcg gtaaatttta
     7681 aaaagttctt tcttatattc ttttcatttt cattcaatta tagattcacc acacttaaaa
     7741 tattcataag atgttgtata aaaacagtcc acttatgtaa aattactaac atcaatgata
     7801 ccatacaaat gccctttaca gtaacgaaac gcccgtgtaa tacacttaat ttaggattga
     7861 ttaatccttt cttcaaaatg atgaatgtgt aatg
//
LOCUS       DAPIDM010000118         7704 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000118 DAPIDM010000000
VERSION     DAPIDM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.118"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(133..1689)
                     /locus_tag="RJE42_002120"
     CDS             complement(133..1689)
                     /locus_tag="RJE42_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDV5877526.1"
                     /translation="MENTNNESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPDTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKCGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYA"
     gene            complement(1860..2792)
                     /gene="arcC"
                     /locus_tag="RJE42_002121"
     CDS             complement(1860..2792)
                     /gene="arcC"
                     /locus_tag="RJE42_002121"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV5877527.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPSFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKDKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            complement(2815..3816)
                     /gene="argF"
                     /locus_tag="RJE42_002122"
     CDS             complement(2815..3816)
                     /gene="argF"
                     /locus_tag="RJE42_002122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV5877528.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDNI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            4246..4971
                     /locus_tag="RJE42_002123"
     CDS             4246..4971
                     /locus_tag="RJE42_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDV5877529.1"
                     /translation="MKKNITNKIVLSTALLLLGTTSTQPPKAPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSFSGKWLWQNPNGTIHATLQTWVWYTHIQVFGPESWGNINQL
                     RDKYVDIFGTKDEDTIEGYWTYDETFTGGVTPAATSSDKPYKLFLKYKDKQQTMIGGL
                     EFYQGNKPVITLKELDFRVRQTLIKNKKLYNGEFNKGQIRITGGGNHYTIDLSKKLKL
                     TDTNRYVKNPRHAEIEVILEKSH"
     gene            5081..5806
                     /locus_tag="RJE42_002124"
     CDS             5081..5806
                     /locus_tag="RJE42_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDV5877530.1"
                     /translation="MKNNLTKKIILSIALTMIGTATSQSPHSPISMSSEAKAYNISES
                     ETNVNELTKYYTQRHLTFSNKWLWQKDNGTIHATLLQLSWFSHIQVFGPESWGNINQL
                     RNKYVDIFALKDYETWRTYMLAQETFTGGVTPAAKPSDKYYKLNVTYKDKAGTFIGEY
                     QFYTGNKPVLTLKEVDFRIRQTLIKNKKLYNGDYNKGHIKITGGSNNYTIDLSKRLKS
                     TDANRYVKNPQTAHIEILLEKPS"
     gene            5915..6631
                     /locus_tag="RJE42_002125"
     CDS             5915..6631
                     /locus_tag="RJE42_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDV5877531.1"
                     /translation="MRKRIMKKLIFMTTLLLLGTTATQTPNSPLNVSTDAKAYHIGQD
                     ETNINELIKYYTQPPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNVNQL
                     RDRYVDVFGLKDEDTSQLWWVYRDTFTGGVTPAASPSDKPYSLFVQYKDKLQTIIGAH
                     KMYQGNKPILTLKEIDFRARETLIKNKILYHENRNKGKLKIIGGGNDFTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
     gene            complement(7132..7359)
                     /locus_tag="RJE42_002126"
     CDS             complement(7132..7359)
                     /locus_tag="RJE42_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877532.1"
                     /translation="MLIEERGLKMTELNEIINAIQSLFESQSGYKISKNSGVPYQTVQ
                     DLRNGKTKIEDARFRTIIKLYSYYTSLKERS"
     gene            complement(7316..7462)
                     /locus_tag="RJE42_002127"
                     /pseudo
     CDS             complement(7316..7462)
                     /locus_tag="RJE42_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2623 a         1398 c         1106 g         2577 t
ORIGIN      
        1 ttaccagtgt tatcattacc gccattattt tcttattcaa aataataatg cacctcgctt
       61 agtttagtat ttctacaaac ctacgagatg cataaaaccc aattagtatt ttttataata
      121 tagcatttca aattatgcat aaattagtac ttgcgtaatt agtactccgc caccgaaaat
      181 caatacaaaa gcaactaccg gccatacaaa tcggaaccaa tgcgaatatc gcatgtttaa
      241 catttgtagt gtggccataa caagtccagt cggcgctaag aataacattg catattgacc
      301 aaattgatat gtcgtaacga tgacgaatct tggtatgcct actgtatcag ctagtggtgc
      361 aaagataggc attgataata ccgctaagcc agaagatgat ggcacgataa aacctaaaca
      421 gaagaatata aatagtaata cgatgataaa taatggtcca ctcatatgtt gaactaaaga
      481 tgatgaaaag tgtaagatcg tatctgaaat cataccttca ttcaacacta aattaatacc
      541 tcgagctaag ccaataatta aagatacacc tactaaactt gatgcaccgt tgacaaatgc
      601 atctacagtt cctttttctc ccaatccaca tttacctgtc ccagcaataa acattattat
      661 aattgtaaat attaaaaatg ctgaagccat aactgggaac caccaacctt gcgtcataac
      721 tccccatacc ataattggaa atggtagtac aaataatgta aggattatct tcttacgcaa
      781 agtaaaatgg gcactatcgt catcttttaa tacagaccat tgctgttcaa aagcatcttt
      841 gtcttcataa gaatatgacg ctttaggatc ttttttaatt tttttacagt accaatataa
      901 ataactaata acaaaaatcg caccgacaat acaagcacct attctccaat acaacccatc
      961 cgtaaaagtt gtaccagcgg cattagaggc aattacaacc gagaacgggt taatagttga
     1021 aaatgtacta ccgacagagc tggcaaggaa tatggcacca acagaaacaa tagaatcgta
     1081 tcctaacgct ataaatatag ggactaaaat cggatagaat gcgacagctt cttcttcaat
     1141 accacacaac gtcccgccga taatcattag tattgataca aacacaatta gcataaattc
     1201 atgccctttt gttttcttag ttaaagctag caatcccgat tcaaaagaac cactggcttg
     1261 aactacgcca ataagtcccc ctaatacaag aatgaatacc atgatatcga ccgcttctat
     1321 cgtaccttca accatgctac ttgttatttg ttctggtcca gctggatgtt gctttaatct
     1381 ttcataagta tccggaattg ataccggctt attaattgca cctgatttaa attgttcaat
     1441 cttaatttta acccccattt tgtctaattc ctgttgtgta cctggaacct ttttcacttg
     1501 gttatgaggg ttaactatct ttagttcttg ggatgaaggt tcgtaagaaa gtttagaata
     1561 tgcaccagca ggaataaccc atgttgctat aactgcaaca accgttaaaa tgaataaaat
     1621 cataaatgca cctggcattt ttaatttaaa gcgttttttc ttttcacttt cattatttgt
     1681 attttccacg gtttcatctc cttcgacatt taacctagca tttctacctt aaagatttta
     1741 taaatataaa ttaagaaagt gcaccccgca tcaaaataga ggcattattt tcaggggtgc
     1801 acttaaataa taaaaatcat gcatttgaca tatagtaatt gaaaagcgtt tcaattcaat
     1861 tactttttaa tcacagtacc tactttaccc tctaaggcag catctaattc atttaatgat
     1921 gttataagca cacttccttt tggattgttt tcaataaatg atatggctgc ttcaattttt
     1981 ggtaacatac ttccttttgc aaattgattt tcgtctatat atcgttttaa ttcatcaaca
     2041 tttgttgttt tcaaaggctg ttggttttca gtgttaaaat taatatatac ataatcaatt
     2101 gctgttaaaa taatcaattg atcgcattga atattagcac ccaacaacgc acttgtttta
     2161 tctttgtcta taactgcatc aataccttta aaaccatcat gttgctctct aattactggt
     2221 atacctccac caccagcagc aataacgagt gtatcatttt taataagtgt tttaatactc
     2281 tctaattcaa taatagagat gggttgtggt gaaggaacaa cacgtctata ccctcttcca
     2341 gcatcttcaa caaatataaa tcctttatct ttttgaattt gttcagcttc ttctttgttg
     2401 taaaataacc caattggttt tgaaggattg ttaaatgccg gatcattttc atcaacttca
     2461 acttgtgtca ctagtgttac cacttgttta tccattccaa tagaatgcaa ttcgttttgt
     2521 aagctttctt gtaattgata tccgatgtaa gcttgactca ttgcgccaca ttcagcaaat
     2581 ggaaatgacg gaccttgatt gtgttctgca gcatagttaa gtcccaaatt aatgcttcca
     2641 acctgtggcc cattaccatg actaataaca atctcatgtc cttttgttat taatcctact
     2701 aatgatttcg cagtattttt aacaagctcg agttgttctt gaggtgattt tcctaaagca
     2761 ttaccgccta atgctactac tattttcgcc atcatattca cttccttata tcatttaaaa
     2821 ttcacccaat gtagcaacca tgactgcttt gattgtatgc attctgtttt cagcttcttg
     2881 gaatacaact gaagctttac tttcgaatac ttcatctgta acttccattt ctcgaatacc
     2941 atatttttca aaaatttgtt gtccaatttt agtatcagca ttgtggaaag atggtaagca
     3001 atgctcaaaa ataacatttg gattaccagt tttatccatc atttctttat ttacttgata
     3061 aggtttcaat aattcaagtc gttctttcca tacttcatca ggttcaccca ttgataccca
     3121 aacgtcagtg taaattacat ccgaaccttt tacaccttgg tcaatattat ctgtgattaa
     3181 tatgttgcca ccattttcag cagcaatatt tttacagcga tttaataatt catctgttgg
     3241 atttaattct tttggacaaa ctaaatggaa gttcataccc ataatggctg caccttgcat
     3301 taatgcattt gcaacgttat tacgaccatc tccaacatat gtaaagttaa tatctgcata
     3361 atcttttttt aagacttctt ttgctgttaa aaaatcagca agaacttgag taggatgatc
     3421 ttcatcagtt aaaccattcc atactgggac acctgaatat tcagctaaag tttctactgt
     3481 tctttgtgaa aaaccacggt attcaatgcc atcatacatt ccaccaagca cacgtgcagt
     3541 atctttagtt gtttcttttt tacccatttg tgatccagtt gggcctaaat aagttacatt
     3601 agcaccttga tcatgcgctg caacttcaaa tgcacatcgc gttcttgtag aatctttttc
     3661 aaataacagt gcaatatttt tattttttaa cataggcttt tcagtgccaa tatatttagc
     3721 acgttttaaa tcctcggaga gtgttaataa gaattctacc tcttgtcgtg aaaagtctaa
     3781 taaagttaaa aaacttctgt ttcgtaaatt tttcattaat aacaactcct tttattttag
     3841 ttacacctaa attgtaagcg catacaaaat aaacacaacc tactattaaa atttgtaata
     3901 ttttatcaat aattaaatga acattttatt aatattaaat ttaagtagta ggaaattatt
     3961 aaaataagta ctacatttaa agtataacta tttttcaagt agttagaaaa ttcaataaat
     4021 aactaattta acgcaattaa tatcataatt accatcagtt ttaatttatt agcactcttt
     4081 tcattttata attggcaatg ttccaatgct taaaaatgaa aaattttact taaatcacac
     4141 gttcaactgt ttatgtttat ttaacatagt gttagctttt atttaattcc gaatcggcgc
     4201 aataccttat atactatggg taattcacgc aaaggagatt atcatatgaa aaagaacatc
     4261 acgaacaaaa ttgttttatc tacagcattg ttacttttag gaactacatc aacacaaccg
     4321 cctaaagcac caatcagttt ttcatctgaa gcaaaagcat acaatataag tgaaaacgaa
     4381 acgaatatca atgaattaat caaatattac acgcagccac acctttcatt ttctggaaaa
     4441 tggctatggc aaaaccctaa cggtaccata catgcaacat tacaaacgtg ggtttggtac
     4501 acgcatattc aagtatttgg acctgaaagt tggggcaaca ttaatcaatt aagagataaa
     4561 tatgtagata tttttggtac caaggatgag gatactatag aaggctattg gacttatgat
     4621 gaaaccttta ctggaggtgt aacaccagca gctacttcat ctgataagcc ttataaatta
     4681 tttttaaaat ataaggataa acaacaaact atgattggtg gacttgagtt ttatcaaggt
     4741 aataaaccag taataacttt aaaagaacta gatttccgcg tacgtcaaac tttaattaag
     4801 aacaaaaagc tatataatgg agaatttaat aaaggtcaaa ttagaattac aggtggtggt
     4861 aatcattata cgattgattt gagtaaaaag ttaaaactaa ctgacacaaa ccgttatgtt
     4921 aaaaatcctc gacatgcaga aattgaagtc attcttgaaa aatctcatta gtatcattca
     4981 ttgagtgaat acaaatagac tgaatgattt aattgatttc tctcttttta taaacttatt
     5041 attcctcgca tataacattc ctcgtaaagg agattttcta atgaaaaaca acctcacaaa
     5101 aaaaattatt ttatcaatag cattgactat gataggcaca gctacaagtc aatcccctca
     5161 ttcaccgatt agtatgtcat ctgaagcaaa agcatacaat ataagtgaaa gcgaaacgaa
     5221 tgtaaatgaa ttgactaaat attacacaca acgccattta acattttcaa ataaatggct
     5281 atggcaaaaa gataatggta caatccatgc aacattgtta cagttatcat ggtttagtca
     5341 tattcaagta ttcggccctg aaagttgggg taatattaat caattaagaa ataaatatgt
     5401 agatattttt gctttaaaag attatgaaac ctggcgcact tatatgttag cccaagaaac
     5461 atttactggt ggtgttacac cagcagcaaa acctagcgat aaatattata aattgaatgt
     5521 aacatataaa gataaagcag gaacatttat tggcgaatat caattttata caggtaataa
     5581 accagtttta actttaaagg aagttgattt ccgaatccgc caaacactaa ttaaaaacaa
     5641 aaaattatat aatggtgatt ataataaagg ccacattaag attacaggtg gcagcaacaa
     5701 ctatacaata gatttaagca aaagattaaa atcaactgat gcaaatagat atgttaaaaa
     5761 ccctcaaact gcacacatcg aaatcctcct tgaaaaacct agctaacatc gaaatcgaag
     5821 taaacagaaa taagcacaat gtttacattg acgccgttca tttttaaatt tgttaattct
     5881 cgtattgatg attatgcaga aaggagaagt tcgaatgaga aagcgtatta tgaaaaaatt
     5941 aatttttatg acaacattgt tattattagg aacaacagct acacaaaccc ctaattcgcc
     6001 attaaatgtt tctactgacg ctaaagctta tcatattggc caagacgaaa cgaacatcaa
     6061 tgagttaatt aaatattaca cacaaccgcc tctcacattt tcaaatagat ggttatacca
     6121 atatgatgac ggcaacattt atgttgaatt taagcgttat tcttggtcag cgcatatacg
     6181 attatggggt gctgaaagtt ggggcaacgt taatcaatta agagaccgct atgtagacgt
     6241 gtttggttta aaagacgaag acactagcca gttatggtgg gtatatcgtg atacatttac
     6301 aggtggtgta acacctgctg catcaccttc tgataaacca tatagtcttt ttgtccaata
     6361 caaagataaa ctacaaacaa ttattggtgc acataagatg taccaaggta ataaaccaat
     6421 attaacattg aaagaaattg attttcgagc acgtgaaaca ctaattaaaa ataaaatatt
     6481 atatcatgaa aatcgcaata aaggtaaact taaaatcatc ggtggcggca atgactttac
     6541 aattgatttg agtaaaagat tacattctga tcttgcaaat gtttatgtta aaaatcctca
     6601 aaaaataaca gtcgaggtcc ttatcgatta aataaggtcg tatgatttat atcgcaaaca
     6661 ctaaggataa acaatagttg attaatacta caaaatgtct caatagtact tacacaatca
     6721 ttaatttagt acacagaata ttaaatgcga tttggagtct aatagaaatg ccaagtcatt
     6781 ctctgtgttg gggcccaccc caacttgcat tgcttgtaga atttctattc gaaattctct
     6841 gtgttggggc cccgcaactt gcattgcttg tagaatttct attcgaaatt ctctgtgttg
     6901 gggccccgca acttgcattg cctgtagaat ttctattcga aattcttttt gttggggccc
     6961 caccccaact tacattgtct gtagaatttc tattcgaaat tctttttgtt ggggcccctg
     7021 tctctaattt aaagtcttgt tataatcgca tttttgtttg gtcaatgtct gtctatataa
     7081 tattgtagag cctaagtcgt ggttatgtct taggctctac tctttattta attatgaccg
     7141 ctcttttaat gatgtataat aactatatag ttttataatt gttctaaatc tagcatcttc
     7201 tattttcgtt ttaccattcc tcaaatcttg tacggtctga tacggcaccc ctgaattttt
     7261 cgatattttg tagcctgatt gagactcaaa caaagattga atagcgttga taatttcatt
     7321 caactctgtc atttttaacc ctctttcttc tattaacaat atacaatgtt gttattaaaa
     7381 cagtcagtgt atgaatgact tcatttccta aaaatatatt cagccataaa ataattagaa
     7441 atgcgattta aaatagtttc attataatcc cctatcattt ttatatttat cactttccac
     7501 ttagtttcaa cattaaaagc taacctaagt ttaagattct cttataacgc ctataactag
     7561 atgtctacat tttcccttgt ataacatatt ttacaatgtt ttataatatt acactacacc
     7621 cttttacatt tttatacgaa agcacaatat tttgttaaaa ttaattcgca caaatattcc
     7681 aagacaatta attttttctc tatt
//
LOCUS       DAPIDM010000119         7682 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000119 DAPIDM010000000
VERSION     DAPIDM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.119"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            160..783
                     /locus_tag="RJE42_002128"
     CDS             160..783
                     /locus_tag="RJE42_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877533.1"
                     /translation="MSNLHLSNIQLIFDDMRDKDYIIDSFKFKYKNENYLVFIRRFKG
                     KFIKRINKFAKVQLTFYKESTMKNSLVVEANNNGLIVNDYNMLYNFFGIESSSFGKGL
                     FPFYESLNEAIPNEVNLNSSKEKNISLSKELYKDDIKEMDKIYCTGVTRLSEGRKRSE
                     RRNEKAKRLRPALYEFFDDHSITFNFSPHEWKEKSDEQIKKDAVNRL"
     gene            966..1856
                     /locus_tag="RJE42_002129"
                     /pseudo
     CDS             966..1856
                     /locus_tag="RJE42_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2070..2729
                     /locus_tag="RJE42_002130"
     CDS             2070..2729
                     /locus_tag="RJE42_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877534.1"
                     /translation="MNRNLLTILLLIGYTYLIFNFLTTEYSLAPRIFYTLNFISLSLI
                     LLAKAKSKKLNKHTHFLDEVQLVLEKRYSTIMYILNIALVVSFISFLTGFIYLREEYP
                     QFILYSSVLLVMSFYYIKLFYEDKNNIIYMNRKIVDFNNSEVECQLQLDDGEKNILLK
                     QLDNFYKSTIILLFITEIMLMFYSIISSNYQTVSMVLIAIVIFISSSLLITKNVYTNR
                     N"
     gene            complement(2779..3165)
                     /locus_tag="RJE42_002131"
                     /pseudo
     CDS             complement(2779..3165)
                     /locus_tag="RJE42_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3241..4212)
                     /locus_tag="RJE42_002132"
     CDS             complement(3241..4212)
                     /locus_tag="RJE42_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV5877535.1"
                     /translation="MTETKAIDTHAHLWNEDYLDKLGKLGSQGTEVAKGINQSASKED
                     LEKRFKMMDDAGVDLQIISATPQSPQWGTKEEAHQSAQEINDLYERLVQQYPDRFLAY
                     GAVSLPYVDQAIEEAQALLKKDEFVGIAIPTIVKDKVSVADKQFEPFFAAMNELNATL
                     YIHPTGCGAQSPLVNDYQLEWVIGAPLESTFITLQLIKNEIPQKYPNIKFHISHLGGA
                     LPFFMTRIKDNYEDWNAFNIDPYEVLNRQFWYDTANFHEPSLINSIETFGKDKFMMGS
                     DFPYFQDEKYTRGVDYIKNSNIDPDTINGILRGNAIEFYQIEENHSH"
     gene            complement(4265..4636)
                     /locus_tag="RJE42_002133"
     CDS             complement(4265..4636)
                     /locus_tag="RJE42_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV5877536.1"
                     /translation="MLLRHVINTYVGSEIFKSSLPKVQNSDEMAQQFEQGFGLSRRSM
                     RLAGAFEMIGSLLLFTSIFGKLGKKLVVVGTIMINIVMGTAIYNHYKAGHGYKGAQAA
                     SKFFFLNVLSLLEVLSLTRNK"
     gene            complement(4656..6047)
                     /locus_tag="RJE42_002134"
     CDS             complement(4656..6047)
                     /locus_tag="RJE42_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDV5877537.1"
                     /translation="MRNIYAFNIDLKQRNRVIAVVMIGAFVGVLNQTLMTTILPEIMK
                     DFTVSSSTAQWLTTIFMLVNGIMIPITAFLIERFTLRSLFFNATCFLMIGSFICMLGI
                     NFPMLLVGRSIQALGAGILMPLTQTLLFIMFPPEKRGMAMGMFGLVIGFAPAIGPTAA
                     GWFVNIFDWRYLFLIVLLIGMIDFVFGYLSLPNITELSKPNLDKLSIILSTVSFGGLL
                     FGFSTAGNLGWSHPMVYITIIAAIVILTVFIFRQLKLESPLLEFRVFKYNDFSVAMIL
                     IVLMFMLFIGNLTILPIYMQTMMKWSPLESGLILLPGGLIMGLLSPVTGKLFDKIGGR
                     ILSIMGMLTIMIGALLMAQFSQNTTQLYVIISFSVTMLGNAMIMTPMTTQALNALPRQ
                     YIAHGTAMNNTIRQVSAAIGTGILVTLMTGLGKTSSLSGSQGLIHGLDITFYVVALIA
                     FIGTIIAFFIRKQ"
     gene            6161..6364
                     /locus_tag="RJE42_002135"
     CDS             6161..6364
                     /locus_tag="RJE42_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877538.1"
                     /translation="MKNAIMKEVVYINFEGLEQYLNGDNTRYNMLSLDVLNYYLKQYV
                     LTIPFKNMDVQNGMKVSAEVKDI"
     gene            6374..6739
                     /locus_tag="RJE42_002136"
     CDS             6374..6739
                     /locus_tag="RJE42_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDV5877539.1"
                     /translation="MNHQHGDFYYEMNNFFNVYFEYKGFTVNIVSSTIHTHGGGKSLK
                     GSHVSLIIPIDGVDYVTGAGFGDLPFSVMPIVQKDISPVIHDMNGDFHAMYVNNYLFY
                     VRKMGKDNDNNWDHTMKRN"
     gene            7014..7589
                     /locus_tag="RJE42_002137"
     CDS             7014..7589
                     /locus_tag="RJE42_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5877540.1"
                     /translation="MEKNQRKKRSDAEYNQQIILTTMEDLLEQGEDISAKKMSDIAKI
                     SGVGVGTLYRHFESKTLLCQAIMDKKVDQMFIEIEDVLAENTQWPVRDKINVILTKYL
                     DLKEANFTTLNFIEKSSSHSSSVINIPFFERLKNLLIQQFENVNSIADLDFKLNLMLN
                     AFSSDFYYFVKHNQKLTKQQFLDKLLDLFLK"
BASE COUNT         2851 a         1120 c         1193 g         2518 t
ORIGIN      
        1 gtgttaaagt gtatttggtg ataaagaaag agagtaaatt aaagtcgata tcaacctatt
       61 ctttttatga gggacattta tgtgagggat aatgccgacc atagagttgt taggataatt
      121 gttgtgtaga attccatttt ttataaggag tagataacca tgagcaattt acatttgtcc
      181 aatatacaat taatatttga cgatatgcgt gataaggatt atattattga ttcatttaaa
      241 tttaaatata aaaatgaaaa ttatttagtc tttatccgca gattcaaagg aaaatttatt
      301 aaaagaataa ataaattcgc gaaagttcaa ttaacatttt ataaagagtc aactatgaaa
      361 aattccttgg tagttgaagc aaataataat ggattaatag taaatgatta caatatgcta
      421 tataattttt ttggcatcga atcatctagt tttggtaaag gtctatttcc tttttatgag
      481 agtcttaatg aagcaattcc taacgaagta aatcttaatt caagtaaaga aaaaaatatt
      541 tctttatcaa aagaactata taaagatgat atcaaagaaa tggataaaat ttattgtaca
      601 ggtgttacac gattatcaga aggtcgaaaa agatcagaaa gaagaaatga aaaagcaaaa
      661 aggttaagac ctgcccttta tgagtttttt gatgaccata gtattacatt taacttttca
      721 cctcatgaat ggaaagaaaa atctgatgaa caaatcaaaa aggatgctgt aaacagactc
      781 taatggtaat aaatatatat aagtaattaa attttattta ttgttgattt gtattttttg
      841 ctaagttaat cataaaaagc aagaagtact atacatataa atttgatatt ttgaaatgtc
      901 tacacctatt agataaattc tatgagagta gatgttgtta atttaagaaa gtagtcgatt
      961 ttattatgac aagaggaaga aaatcgttta gttcagagtt taagttacaa atggttagat
     1021 ttcattccta ggtgttagga aaatggataa agcaacataa aaacatgggt acattcaatc
     1081 accaagataa cttatcagat gaagaaaaag agctgattaa attacgtaaa gaagttcaat
     1141 atataaaaat ggagaacgat attttaaagc aagcagcgct gatcatgcga cgaaaataga
     1201 agtcattcaa aaaaatgtac atcaacattc agtaccagca atgtgtaaag tcctaaaaat
     1261 accaagaagt acctattata attctataaa aagaaaagat acaaaaatca tccaaaagaa
     1321 actaatgaaa aacgaattaa aaatcatttg aatcgcgctt ttaatagaga gcaaccaatg
     1381 gagacattag taagtgattt aacatatgta aaagtcgtag gaacatggca atacatatgt
     1441 ttatttatag atctatttaa tagagaaatt gttggttaca gtgcaggaaa agtaaggatg
     1501 caaatatagt atcaaaagca atcagcagaa taaatcataa tcttgaacaa atcaaactat
     1561 tccaaaccga tagaggcaaa gaatttaata atcatttgat agatgaagtc ctagaaacct
     1621 ttaaaatcaa acgttcatga agtaccaaag gttgtcctta tgataacgca gttgcggaag
     1681 cgacgatgaa agcaatgaaa actgaatttg taaaacaaat gtaatttgaa aatttagaac
     1741 agttagaaac agaattattt gattatgtaa attggtacaa caattttaga ccacattctt
     1801 cattaaaata tttgacgtca gtggcattta aaaatctaca catgaaaact gtctaaaaaa
     1861 tgttgacata ccatagtaaa attaaaggag ttgaaagaaa agtttaatat ttcacaaact
     1921 gagctgtcta aaaaaaacta atatatgtag gcaaactata aacacgttag aaaatggctc
     1981 atatttaccg tcgcttaaaa ccttaaaaaa atatcattat tctttaatac tagtatagaa
     2041 agcatcttta tatttgagga ggaagattta tgaacagaaa tcttttaacc attttattat
     2101 tgatagggta tacgtattta atatttaact ttctaacaac tgaatactcc ttggcgccaa
     2161 gaatattcta tacattgaac tttattagtt tgtccttaat attattagca aaggctaaaa
     2221 gtaagaaatt aaataaacac acacacttcc ttgacgaagt acaactagtt ttagaaaaaa
     2281 gatattccac tataatgtat atattgaata tcgcgttagt agtttccttt atttcattct
     2341 taactggttt tatatattta agagaagaat acccacaatt tatcctttat tcaagtgtat
     2401 tgctagtaat gtctttctat tatattaaac tattctatga agataaaaat aatattattt
     2461 atatgaatag aaaaattgtg gattttaata actcagaagt agaatgtcag ttacagttag
     2521 atgatggaga aaaaaatata ttattgaaac aacttgataa tttttataaa tcgaccatta
     2581 ttttattgtt tattaccgaa ataatgttga tgttttactc cataatatca tctaactatc
     2641 aaacagtgag tatggtttta attgctatag ttattttcat ttcatcttca ctactaatta
     2701 caaagaatgt atatacaaat cgcaactaat taataatggc tctatgtcaa attggactga
     2761 tgcattcaat aaaggaagtt aggcaactaa acattgctta acttcctttt tactttttag
     2821 agcgtaaagt tttaaacata ataatatacg attgcgcaaa tgattgtaat ttccataacc
     2881 aaaagacgtg cgtttaatta attttatttt gttgtttatc ccttctaaag gaccatttga
     2941 taaattgtaa taatcaaatg tattttttat atagtataca tgtttgttaa gtgtcttaat
     3001 tgctcgttgg acaactgaat gcgtttggga taatggcata tgttttaatt cgtgaataaa
     3061 gccttcataa tcattatgtt taagtttgaa tcatagctga ttgatatatt ggtaggcgtc
     3121 atttagtgag tcatcttgat ctaataaata cttaatgata cctttttata tagaaagtaa
     3181 agtagcgtta ttctaactta tttagtttat accataatat aatgcctcta gaataaaacg
     3241 ctagtgacta tggttttctt caatttgata aaattcaatt gcattgcctc ttaatatacc
     3301 attaatcgtg tcagggtcaa tattactgtt tttaatatag tctacgccgc gtgtatattt
     3361 ttcatcttgg aagtaaggga agtctgaccc catcataaat ttatccttac caaatgtctc
     3421 aatagagtta atcaatgaag gttcgtggaa gtttgctgta tcataccaaa attgtcgatt
     3481 caatacttca taaggatcaa tattaaaggc attccaatct tcataattgt ctttaatacg
     3541 agtcatgaag aatggtaaag cgccacctaa atgtgaaata tggaatttaa tatttggata
     3601 tttttgtgga atttcatttt taatcaattg taatgtaata aaggtagact ctaacggtgc
     3661 accaataacc cattctaatt gatagtcatt gactaacgga ctttgagcgc cacaacctgt
     3721 tgggtgaata taaagtgtag cattgagctc attcatcgct gcaaagaaag gttcaaattg
     3781 tttatcagca acagagacct tatcttttac tatagttgga atggcaatgc ccacaaattc
     3841 atcttttttc aaaagggctt gggcttcttc aatagcttga tcgacataag gtagtgagac
     3901 agcaccatat gccaagaaac gatctggata ttgttgtact aatctttcat ataagtcatt
     3961 aatttcttga gcactttggt gagcttcttc tttagtaccc cattgtggag attgtggtgt
     4021 cgcagaaata atttgtaaat ctacgccagc atcgtccatc attttgaagc gtttctctaa
     4081 atcttcttta gacgcagatt gattaatccc tttagcaact tcagtacctt gactacctaa
     4141 tttgccaagt ttgtctaaat agtcttcatt ccataaatgt gcgtgtgtat caatcgcttt
     4201 tgtttcagtc atattaaccc ctccattaaa aattgtcatt cataatttgc ttattgccaa
     4261 tagattattt attacgagtt aagcttaaca cttctagtaa gcttaataca tttaaaaaga
     4321 aaaatttaga agcagcttgt gcacctttat aaccatgccc agctttataa tggttataaa
     4381 tagctgtacc cataacaata ttaatcataa tcgtaccaac aactactaat tttttcccta
     4441 atttaccgaa aatagaagta aataaaagaa gtgaaccaat catttcgaaa gcgccagcta
     4501 acctcattga tctacgtgat aaaccaaagc cttgttcgaa ttgttgagcc atttcatcac
     4561 tattttgtac ttttggtaaa ctacttttaa aaatttcact acctacatat gtgttaatga
     4621 cgtgtcttaa taacatttta aaatcctcca attaattact gtttgcgaat aaagaatgca
     4681 atgatagtac ctataaaggc aatcaatgcg acgacataga atgtaatatc tagaccatga
     4741 ataagtcctt gactaccact taatgaagag gttttaccta atcctgtcat caatgtgacc
     4801 aagataccag tacctattgc agctgatact tgtctaatcg tattattcat ggctgtacca
     4861 tgtgcaatgt attgacgtgg caaagcattt aaagcttgag ttgtcatggg cgtcatgatc
     4921 atagcattac ctaacatggt aactgaaaat gaaataatca catatagttg tgttgtattt
     4981 tgactaaatt gagccattaa taatgcgcca atcataatcg ttagcatccc cataatactc
     5041 aagatacgtc caccaatttt atcgaataat ttaccagtga caggcgataa cagtcccata
     5101 attaacccgc caggtaataa aataagtcct gactctaacg gagaccattt catcatcgtt
     5161 tgcatataaa ttggtaagat tgttaaattc cctataaata acatgaacat tagtacaatt
     5221 aaaatcattg ccactgaaaa gtcgttatat ttaaatactc taaactctaa caatggcgat
     5281 tccagtttta attgtcggaa aataaatacc gttaatataa ctatggcagc aataatagtt
     5341 atataaacca ttggatgtga ccaacctaag tttcctgctg tactaaaacc aaatagtaag
     5401 ccgccaaagc taaccgtcga taaaataatg gatagtttgt ctaagtttgg tttacttagt
     5461 tctgttatat ttggcaatga tagatacccg aatacaaaat caatcatacc tattagtaat
     5521 acgattagga acagatagcg ccaatcgaaa atattgacga accaaccagc tgcagttggt
     5581 ccaatcgcgg gtgcaaaacc tattacaaga ccgaacattc ccattgccat tccacgtttt
     5641 tcaggtggaa acataatgaa aaggagtgtc tgtgtaagtg gcatcagtat accagcacct
     5701 aaggcttgaa tactacgccc tactaataac attggaaaat tgattcctag catacaaata
     5761 aatgaaccaa tcattaaaaa gcatgttgca ttaaaaaata aagaacgtaa tgtaaaacgt
     5821 tcaattaaaa aagctgtgat aggaatcata atcccattga ctagcataaa aattgtcgtg
     5881 agccattgtg ctgtactact tgataccgta aaatctttca taatttctgg aagtatcgta
     5941 gtcatcaaag tttgattcag cacaccaaca aaagcaccaa tcattacgac agctataaca
     6001 cggttccgtt gtttcaaatc tatattaaag gcatatatat tcctcaaatt ttgacgcctc
     6061 cccctctttt aagtggaacc tactatcatt tattttatac ggaatgggtt ccgtttgtca
     6121 atttaataac tcgagggtat gataataggt atctgataaa atgaaaaatg caataatgaa
     6181 ggaggtagtt tatataaatt ttgaaggatt agaacaatat ttaaatggtg ataatacaag
     6241 atataacatg cttagtttag atgtgcttaa ttattattta aaacaatatg tgttaactat
     6301 cccgttcaaa aatatggatg ttcaaaatgg gatgaaagtt tctgcagaag taaaagatat
     6361 ttaaaataaa gtagtgaacc atcaacacgg tgacttttat tatgaaatga ataatttctt
     6421 taacgtgtat tttgaatata aaggatttac tgtaaatata gtttcttcaa ctatccatac
     6481 ccatggtggt ggaaaaagct taaaaggttc tcacgtgtct ttaatcatac ctattgatgg
     6541 cgttgattat gttacaggtg ccggctttgg agacttgcca tttagtgtca tgccaattgt
     6601 tcaaaaagat attagtccag tcattcatga tatgaacggt gatttccatg caatgtacgt
     6661 caacaattat ttattttatg ttcgaaaaat ggggaaagat aatgacaata actgggatca
     6721 cactatgaag cggaattaac acctcgtcat attcatgact ttgtttacaa tattgaatat
     6781 aatgaaatta attctaattc tatttttgtg aaacgcttgt tagtaacaat gattaaatct
     6841 ttttggtcgt gctacaatgt tacaaaataa tataacttta actaagcaac aaaagaaaga
     6901 ataatatgat gtaacacctg agaatgacag acaattttta gaagaagcat ttaatttaga
     6961 tgttaaaatt gatagattag aaagttaaaa acaacgttaa aaagaggaat ataatggaaa
     7021 aaaatcaacg aaaaaaacgc agtgatgccg aatataacca acagattatc ttaactacaa
     7081 tggaagattt actagaacag ggtgaagata taagtgctaa gaaaatgtca gatattgcta
     7141 aaatatcagg tgttggagtt ggtacattat atcgtcattt tgaaagtaaa acattgttat
     7201 gtcaggctat tatggataaa aaagtcgatc aaatgtttat agaaatagaa gatgttttag
     7261 cggaaaatac acagtggccc gtgcgagata aaatcaatgt tatattaaca aagtatctag
     7321 atttaaaaga agcaaatttc acaacattaa attttataga aaaatccagt tcacactcta
     7381 gttctgtaat taatattcca ttctttgagc gactaaaaaa cttattaatt caacaattcg
     7441 aaaatgttaa ttccatagca gacttggatt ttaaacttaa tctaatgctc aatgcatttt
     7501 cttcagattt ttattatttt gtaaaacata accaaaaatt aacaaaacaa caatttttag
     7561 ataaattact agatttattt ctaaaataat atgttctgaa aaatagaagt attttttgaa
     7621 ccactaaata acgcgctatt aaatggcttc gctatgcccg tctaacacat tagtgtaaaa
     7681 tt
//
LOCUS       DAPIDM010000120         7675 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000120 DAPIDM010000000
VERSION     DAPIDM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.120"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..411)
                     /locus_tag="RJE42_002138"
     CDS             complement(<1..411)
                     /locus_tag="RJE42_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877541.1"
                     /translation="MCFDINNLNIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYSEPYKKTNVIKKFTSLTIDELYYFKEY
                     KKEKDDNILCEEFSYKCSIKDIKYKKLLKAISNQD"
     gene            complement(672..797)
                     /locus_tag="RJE42_002139"
     CDS             complement(672..797)
                     /locus_tag="RJE42_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877542.1"
                     /translation="MTYIHFSIITLITGIIMQMTMYFVKFECRKMPLFMTIIFTI"
     gene            complement(1016..1864)
                     /locus_tag="RJE42_002140"
     CDS             complement(1016..1864)
                     /locus_tag="RJE42_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV5877543.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEVSGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRKVDV"
     gene            complement(1934..2350)
                     /locus_tag="RJE42_002141"
     CDS             complement(1934..2350)
                     /locus_tag="RJE42_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDV5877544.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDVNIERLWERK"
     gene            2594..2761
                     /locus_tag="RJE42_002142"
     CDS             2594..2761
                     /locus_tag="RJE42_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877545.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLHTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            2758..5187
                     /locus_tag="RJE42_002143"
     CDS             2758..5187
                     /locus_tag="RJE42_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDV5877546.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYNKYDENNDAMKKKFEATEKEAEKLLSSMKTESGRTYLWA
                     GAENLETNSSHMTRTYRNIEKIAEAMKHKNTKLKTDENKTKVKDALEWLHENAYGKEP
                     DKKVADLKTNFSKSAPQKNTNLNWWDYEIGTPRALTNTLILLNGDISSDEKKKYTAPI
                     KTFAPKSDEILSSVGKAEPAKGGNLVDISKVKLLESIIEEDTTMMKESIVAFNKVFTY
                     VQSNATDKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNN
                     TTLKSWIDDGFLPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDTMDDST
                     KTKYKQIIKTSVKSDSSYNQNDYLNSYSDIDKMKKLIDDKSITTNDLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMKRLAGTTTLDNEEPKSTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAK
                     KSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSV
                     FLESTNKPKNNIGYHFLNKPKITVTKETHTGNWKEINKSQKDTQKTDEYYEVTQKHSN
                     SDNKYGYVLYPGLSKDVFKSKASQVTVVKQDDDFHVVKDNESVWAGVNYSDSTQNFEI
                     NGTKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTT
                     NESGVRFELTK"
     gene            complement(5247..6101)
                     /locus_tag="RJE42_002144"
     CDS             complement(5247..6101)
                     /locus_tag="RJE42_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDV5877547.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWHFRKLFKSYTR
                     QSDVHELKVCTQNDGTMEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEFFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFLNNLELIKGD
                     VVCGLQGE"
     gene            6973..7251
                     /locus_tag="RJE42_002145"
     CDS             6973..7251
                     /locus_tag="RJE42_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877548.1"
                     /translation="MTLNEYNYNNELILKLNKIEVQKNNEKISEVFKDYEILKPKIVF
                     NGSNPYLALNHIIVAEIENYSSLETFSNIYVDEDYYYNYGELIIKCKK"
     gene            7261..>7675
                     /locus_tag="RJE42_002146"
     CDS             7261..>7675
                     /locus_tag="RJE42_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877549.1"
                     /translation="MTTYMLMYSIPEKAGGITSVMLNRSKLLAEKGYSCALLTLDDKN
                     YDPLRAKLTNSGRLHKQVKIVNLYEELKMLYDVENTEIENKNTQTFEKLNLLNQEGFY
                     IQKDEYEEKKYARYFKDNHYIMYKKWINGQLSHIDY"
BASE COUNT         2697 a         1227 c         1086 g         2665 t
ORIGIN      
        1 gtcttgattg gatatagctt ttagtaattt tttatatttt atatctttta tggaacactt
       61 atatgaaaac tcctcgcaca aaatattatc atccttctcc tttttatatt ctttaaagta
      121 atacaattca tctattgtta acgatgtaaa tttttttata acatttgttt tcttataagg
      181 ttcactataa acatatacta catgaaccaa atttatagaa tcagaaggcg aaaacaaatc
      241 ttcaaaaatg tttattgcat tatcatatac agctaaaaaa taagtattat tgatagtttt
      301 accgtcctcc aaaaactggt atttatcgcc acttaaactt aaatgaattg cgttttttac
      361 ctttctaaaa ttcaactttt ttatattcaa attatttata tcaaagcaca taatttacaa
      421 caccttattc ccttaagatt tttccttctt tagttaaaga agttattctt ttcctttgtt
      481 atttattaac ttccaataag aaccaccatg tgattgccct ttatttttgg aaatagtcca
      541 tttagttttt ggatctctcc aggtaggatt tttcccttaa accttttgag taaatttacc
      601 aatactcatc cccttatcat ttaacaaact tttattaatg tttttggtta tattatgagt
      661 ctttactgct tttagattgt aaaaataatc gtcatgaata atggcatttt ccgacattca
      721 aattttacaa agtacattgt catttgcata atgatacctg taattaatgt aataatagaa
      781 aagtgtatat atgtcatatc gccacctgct atacaactta ttaaataatt aattccaaga
      841 catattaatt atctaattat aaccttagtt aagttattgc atagtacaaa aatatataat
      901 aacttatttg cttgctcctt taacttttta agagtaaaaa ataacctgaa acatagactt
      961 taacatccaa gtttcaggtt agtgtaatgt aaattattta cttgttatat tatgattaaa
     1021 catctacttt tctgcttgta agtgctttaa tcatctctgg atcagcatgt gagaaaaatt
     1081 ggctattact ttcttctaat gtcgcaattt gttgtttatc ttcttctgta agttcaaaat
     1141 caaatatatc taagttttga gccatacgtt ctggattcac tgattttgcg agtacaacga
     1201 tgtcacgttc tactaaccag cgtaaaataa cttgtgcaat tgatttgtta tatttatctg
     1261 caatggcctg caacaattga ttttggaata attgattttt accttctgca aatggtgccc
     1321 aagcttctac cacaacattt tcttgttgaa gtgctgcaac ttgttcttct tgttgatgga
     1381 atggattaat ttctatttgg ttcacttgtg gttgaatttg attgtgaata cctaaatcta
     1441 caattcgatc aacgccgaag ttagatacac cgattgcttt aattttacca ttttctttta
     1501 actcttctaa tgcacgccat gagccatata catcattata aggttggtga ataagcacta
     1561 gatcaagata gtccaaattt aatcgttgta atgatcgttc tattgatttg atagtatctt
     1621 catagttaac attttcaatc catactttag tcgtaatgaa tagttcactt ctatcaatac
     1681 ctgaaacttc aataccttga cctacttcag tttcatttaa ataactttgt gctgtatcaa
     1741 tatgtctata acccgcttta attgcttctt taaccgcttc tgctgtttgt tcttgaggaa
     1801 tttgaaatac tccgaaacct aatactggta tctttacatc tttacttatt tcaatatgat
     1861 tcataaaatg aacctccttt atcttgtaag tccactataa accatggagc aaactctata
     1921 gcaagccaga ggtttacttt ctctcccaaa gacgttcaat attgacatca tgattatcac
     1981 tagcattgat tttagcaata tgattatcat aattatcaat tttatattgt agtaaatctc
     2041 tcgcatcatg aattgtcttt aagttttcat ttaattcttc gagttgctta tttaaaattt
     2101 gcttttgctg tgcttgaata ttttcatttt taggaagctg cgctaatcta caaaattcga
     2161 taagcgattc aatactgacg cctgcatttc gcaaattttt cactaaataa atccaattta
     2221 aatcactatc attatatatt ctatatccat tttcatctcg attaactggt ggaatcacac
     2281 caaccttttc ataatatctt aaagtgtctt gagatatatt catgagcgct acgacttctt
     2341 tagttttcat tgcgactatc ctttcagtta tatttgggca tctaaagtaa tgttgcttta
     2401 tatattgtca tctttgtttg aatacttctt attttattac tcaaatttaa atttgtctct
     2461 tttttaacat tttacatttc atcgttttta attgttttaa aaattgtata acttaaatat
     2521 ttaaaatgat ataatcacta agattgataa tatttaattt tttaaaaatt attttcgttg
     2581 caatttttgg gtgatgcgga aaatttgggt gatcgattgt ttatatgcat caacattctt
     2641 tgtggattgt ttgacagtag acagtcattt gcatacgtta acgaattttg aaaaattgaa
     2701 tcattattta atcataatat tcattgcgca cgcgcaacac aaaaaggaga catgtcaatg
     2761 acatatagaa tgaagaaatg gcaaaaatta tctaccatta cgttattaat ggctggtgtg
     2821 attactttga atggtggtga attcagaagt gttgataaac atcaaatcgc tgtggctgat
     2881 acgaatgttc aaacgccaga ttatgaaaag ttgaggaaca catggctaga tgttaactac
     2941 ggttataata agtatgatga gaataatgac gcaatgaaga agaagtttga ggctacggag
     3001 aaagaggcag agaaattact cagtagcatg aaaactgaaa gtggaaggac ttacttgtgg
     3061 gcaggagcgg aaaaccttga aactaattct tctcatatga ctcgtaccta tcgtaatatt
     3121 gagaaaatcg cagaagcgat gaagcataaa aatactaaat taaaaacaga tgaaaacaag
     3181 acaaaagtaa aagatgcact tgagtggttg catgaaaatg cctatggaaa agagccagat
     3241 aaaaaagttg ctgatctaaa aactaatttc tcaaaatcag cacctcaaaa gaataccaat
     3301 ttaaattggt gggattatga aattggaaca cctagagcat taacaaatac cctaatatta
     3361 ctgaatggtg atatttcaag tgatgaaaag aaaaaataca ctgcccctat taaaactttc
     3421 gcaccaaaaa gtgatgaaat attatcttct gtaggaaaag ctgaacctgc taaaggcgga
     3481 aatttagtag acatttctaa agtaaaactt ttagaaagta ttatcgaaga agatacaact
     3541 atgatgaaag aatcaatagt tgcatttaat aaagtcttca cttacgttca aagtaatgca
     3601 actgataaag aacgtaatgg attttataaa gatggttcat atatcgacca taaagatgtc
     3661 ccgtacactg gtgcttatgg cgttgtactc ttagagggga tttctcaaat gatgccgatg
     3721 ataaaagaaa caccttttaa tgataaaacc caaaataata caaccttaaa atcatggatt
     3781 gacgacggat ttttgccact catttacaaa ggtgaaatga tggatttatc acgtggtaga
     3841 gctatcagtc gcgaaaatga aacgagtcat acagcatctg caacagtaat gaaatcattg
     3901 ttgagattga gtgataccat ggatgattca acaaaaacaa agtataagca aatcatcaaa
     3961 acttctgtta aatctgattc aagttacaac caaaatgatt atttaaattc atattcagac
     4021 atagataaaa tgaaaaagtt aattgatgat aaatctatta ctactaacga tttaacacaa
     4081 caacttaaaa tatataatga catggatcgt gtcacctatc acaataaaga cttagacttt
     4141 gcatttggtt taagtatgac gtcgaaaaac gtcgcacgtt acgaaagtat caacggagag
     4201 aacttaaaag gttggcacac tggtgctgga atgtcttatt tatacaatag cgatgtgaaa
     4261 cactaccgtg ataacttctg ggcgacagct gatatgaaac gtttagcagg tactacaact
     4321 ttagataatg aagaacctaa aagtacggat gttaaaaagt ctagtaaaac ttttgtagga
     4381 ggaacaaaat tcgatgacca acatgctagt atcggaatgg attttgaaaa tcaggacaaa
     4441 actttaactg ccaaaaaatc atatttcata ttaaacgata aaattgtctt cttaggaact
     4501 ggcattaaaa gtactgattc atcaaagaat ccagttacaa cgattgaaaa tcgcaaagcg
     4561 aatgggtata cgttatatac agacgataaa caaacaaccg cttccgataa tcagggaacc
     4621 aattcagtct ttttagagtc aaccaataaa cctaaaaaca acataggcta tcatttttta
     4681 aacaaaccga aaataactgt aacaaaagaa actcatactg gaaattggaa agaaataaat
     4741 aaaagccaaa aggatacaca aaaaactgat gagtattatg aagtaactca aaagcattct
     4801 aattctgaca ataaatatgg atatgtgttg tatccaggct tatctaaaga tgtctttaaa
     4861 tccaaagcaa gccaagtaac tgtcgttaaa caagatgatg acttccacgt tgtgaaagat
     4921 aatgaatcgg tttgggctgg tgttaattac agcgatagca ctcaaaattt tgaaattaat
     4981 ggtactaaag tcgaagttaa agccaaagga atgtttattc ttaaaaagaa agatgataac
     5041 acttatgaat gtagcttcta taatcccgaa tctacaaatt ccgcttcaga tattgaatct
     5101 aaaatttcaa tgactggata ctctattaca aacaaaaata catcgactac aaatgaatcc
     5161 ggcgtgcgct ttgaactaac taaataagca agaaaatctt ggcactatac catttttata
     5221 gtgtcgagat tttataagta tcgccatcat tcaccttgta atccgcagac cacatcccct
     5281 ttgataagtt ctagattatt taaaaatcga attttcaaag acgtacatgc ttcaatattc
     5341 ttatcattca ttacttgaaa atgtatatga ggttcagtag aattgccaga attcccaacc
     5401 ttcccaatga tgtcaccata tttaacattt tgcccctcat tcacaatgat tgaatattgt
     5461 tgtaaatgag ctattaggct atactcattt tctgcgtgct tgattacaat ataatttcct
     5521 aaaaactgtg attcatttgt gcttccaata ctgttgtctt gaaccccatt cactactttt
     5581 tcaactgtac catttgctgg cgcaatgata tcttttccaa aacaaaaata atcagtattt
     5641 acttttcctt ggttcttata cgttttatga ttttctttaa taactaaatc aatagcatac
     5701 ctttgatgtg ggtataaata atgataatta tataattcat tatctccgcc ccaaaaaaac
     5761 tcatagcaat ctgttgtcgg cactgcgtat tttaaagtcg tttcattctt aatagcatca
     5821 taattgatta acggacgcat aacaaatcca ataatactat tatctttcca agtcactgcc
     5881 actccaaatt gatttgcttt atctatccat atttgctcca tagtaccatc attttgagta
     5941 cacactttta actcatgtac atcagactgc cttgtataag atttgaaaag tttcctaaag
     6001 tgccaataac taaactctcg cttcaaattt tgatcaaaaa tcttatatat tttcctgctc
     6061 ttacttttat ttaaataatc cccaaagttt gttttattca tatattctta acctcttatg
     6121 tttattttgg tatcaattct ttctccagtt cctgcgtcat ttttatgcta ccttacattt
     6181 tttgattccc ctggaaattt tgtgaatttg cttttttaca tttctcgtcg aatatgttgg
     6241 aatgaatatt cacgtttttt tcatcatcct attcgctttc agagacacaa aatacctcgc
     6301 acatcttcgt tgcgtgaatc ttgtggaagt ttttgtctat gaaatcaaaa ttgcttttta
     6361 ttctttatta aagatgtgca taaccttttt ttattatatg ttcttctaaa actttaagtc
     6421 tttttgatag attcacatca ttttccaatt atctgcaaaa gcatttcctc tatttttata
     6481 acatttagtt aaccactttg atattttttg aataggaatt taaagacgca ttaagacatc
     6541 agtaacattt atacttgata caacgagttg tattacttcc attttaaagt atattcacat
     6601 ttttctctta gtcatgacgg tcagtcctac aatttgaaat ttttattgca acttagtcac
     6661 tgtcaattcg ctgtgttcga taatcagttt accatcaata agtatttcaa actaaaaatt
     6721 tttaaataga aagaatctag ttcctcataa tatcctgtca atacttcatt acattattta
     6781 tctttagtag cttttacagc atatcagctg aaaagttcca acaacattga caaccaacct
     6841 aataaataag aaaatataat tgtacataac ctaaaatgct ataaataata aaatgatagt
     6901 atctatttac ttagataaat agatgggcat cgaataaaat tttaatgatg gagaaaacag
     6961 ggatttatca acatgacatt aaatgagtat aattacaaca atgaattaat attaaagtta
     7021 aacaagattg aagtacaaaa aaataacgaa aaaatttctg aggtttttaa ggattatgaa
     7081 atattaaagc ccaaaattgt ttttaatggc tctaatcctt atttagcatt aaatcatatt
     7141 atagttgctg aaatagaaaa ttacagttct ttagaaacgt tctccaatat atatgtagat
     7201 gaagattact actataatta cggagaatta attatcaagt gcaagaaata ggaggaaatt
     7261 atgactactt atatgttaat gtattcaata ccagaaaaag caggtggtat aacatcagtt
     7321 atgttaaata gatctaaact tttagctgaa aaaggctatt catgcgcctt attaactttg
     7381 gatgacaaaa actatgatcc attaagagct aagctaacaa attcaggacg gttacataaa
     7441 caagtaaaaa ttgttaactt atacgaagaa ttgaagatgc tgtatgatgt tgaaaataca
     7501 gaaatcgaaa ataaaaatac gcaaacattt gaaaaattaa atcttttaaa tcaagaaggc
     7561 ttttatattc aaaaagacga atatgaagaa aaaaaatatg ctagatactt taaagataac
     7621 cattacataa tgtataaaaa atggataaat ggtcaattat cacacattga ttatt
//
LOCUS       DAPIDM010000121         7573 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000121 DAPIDM010000000
VERSION     DAPIDM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.121"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..397
                     /locus_tag="RJE42_002147"
     CDS             <1..397
                     /locus_tag="RJE42_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877550.1"
                     /translation="IWIGIVVQICYNVVIVSMTTFIFFINTEGASPEIAVLIALVIIL
                     ILTLPPILTVINLENKMEVKGTLFIVYAIVAFCMFNYLSSILWVVCGIFLIWTKYSKG
                     GSTGKNENEKVEIESTENQFESKDKTTKE"
     gene            447..1073
                     /locus_tag="RJE42_002148"
     CDS             447..1073
                     /locus_tag="RJE42_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5877551.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISSIISIFVIILSIVPVIVLASDLFKERISNGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVSTSDTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEIHKDEATTKVDSDNTEPPFESKDHVSKKD"
     gene            1285..2061
                     /locus_tag="RJE42_002149"
     CDS             1285..2061
                     /locus_tag="RJE42_002149"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDV5877552.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2396..3376
                     /locus_tag="RJE42_002150"
     CDS             2396..3376
                     /locus_tag="RJE42_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV5877553.1"
                     /translation="MEDLEKVLITGGAGFIGSHLVDFLSQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLETIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAVGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHDFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            3339..4028
                     /locus_tag="RJE42_002151"
     CDS             3339..4028
                     /locus_tag="RJE42_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDV5877554.1"
                     /translation="MKKLQRKKWKCREIGIEAVHNKGYAYQRKLDTLEEVKKGYFPIK
                     RAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMCK
                     NAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVEL
                     FSSEIIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIRT
                     LMVVITGEGSR"
     gene            4238..5404
                     /locus_tag="RJE42_002152"
     CDS             4238..5404
                     /locus_tag="RJE42_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV5877555.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCIHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINDSTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICIV
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            5385..6623
                     /locus_tag="RJE42_002153"
     CDS             5385..6623
                     /locus_tag="RJE42_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDV5877556.1"
                     /translation="MENQHNSKYLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMIYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6613..>7573
                     /locus_tag="RJE42_002154"
     CDS             6613..>7573
                     /locus_tag="RJE42_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV5877557.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAIYM
                     VVITIFIDDYYLVFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASVIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFAS"
BASE COUNT         2543 a         1071 c         1389 g         2570 t
ORIGIN      
        1 aatttggata ggcatcgtag ttcaaatatg ttataacgtc gtcattgtta gcatgacaac
       61 cttcatcttt tttataaata ctgaaggagc aagccctgaa atagctgttt taatagcatt
      121 agtaatcatt cttatcttaa ctttaccacc tatcctaacc gtgattaact tagagaataa
      181 aatggaagta aaaggtacgc tttttattgt ttatgcgatt gtggcatttt gtatgtttaa
      241 ttacctttca tcaattttat gggtggtttg cggcattttt cttatttgga ctaaatattc
      301 aaaaggtggg tcaacaggta agaatgagaa tgaaaaagtt gaaattgaaa gtacagagaa
      361 tcaatttgaa tctaaagata aaaccactaa agaataaaga gaatatttaa ggcggagtat
      421 aaatattaaa taaatgggga atagacatgg aaaaaaatgt agaaaaatca ttcataaaga
      481 taggtttata ttttcaaata gcttatatag tactcatggc tataacttta tgtgggtttg
      541 taatttgcta tggactaatt ttcggccttt tctatttatt gtcaggtagc agagctgatt
      601 atttaatagt aacgatagtt atatcgtcaa taatttctat atttgtaatt atactttcaa
      661 tcgtacctgt catcgtattg gcatctgact tatttaaaga aaggatttca aatggtgtca
      721 tattaattgt attggctatt atcgctttag tattatgcaa ctttgtatct gcaatactct
      781 ggtttgtttc agccatatct attttaggta gaaaaaaatt agtatctaca tcagatacta
      841 ccactattca aaaaagtaaa gggaacgcaa atcaagcatc acataaagac acgtgtaaaa
      901 aggaacttga tagtcaagac atgatggaac atcctgagtt taaaaatccc acgactaaaa
      961 acctagaagg attaaacgaa gaaatacata aagatgaagc tacaactaaa gttgacagtg
     1021 ataacacgga accgccgttt gaatcaaaag accatgtctc gaaaaaagat tgatgacaaa
     1081 ctaatcgaga gacttaaaaa aataatattc aacataagca cttttaaaac gacatttaaa
     1141 cgcattgcca atcactaatg gtagtgcgtt taactatacc ttaaatatct gaatattttg
     1201 ttaaatggag ttacctttgt tgtactattc aaatgaagag gagtaaaatg taattaaagg
     1261 aaagtaattt gaggagtgat ctttatgaca aacaacaaag tagcattagt aactggcgga
     1321 gcacaaggga ttggttttaa aattgcagaa cgtttagtgg aagatggttt caaagtagca
     1381 gttgttgatt tcaatgaaga aggtgcaaaa gcagctgcac ttaaattatc aagtgatggt
     1441 acaaaagcta ttgctatcaa agcagatgta tcaaaccgtg atgatgtatt taatgcagta
     1501 agacaaactg cagcacaatt tggcgatttc catgtcatgg ttaacaatgc cggtcttgga
     1561 ccaacaacac caatcgatac aattactgaa gaacagttta aaacagtata tggcgtgaac
     1621 gttgcaggtg tgttatgggg tattcaagcc gcacatgaac aatttaaaaa attcaatcat
     1681 ggcggtaaaa ttatcaatgc aacatctcaa gcaggcgttg agggtaaccc aggcttgtct
     1741 ttatattgca gtacaaaatt cgcagtgcga ggtttaacac aagtagccgc acaagattta
     1801 gcgtctgaag gtattactgt gaatgcattc gcacctggta tcgttcaaac accaatgatg
     1861 gaaagtatcg cagtggcaac agccgaagaa gcaggtaaac ctgaagcatg gggttgggaa
     1921 caatttacaa gtcaaattgc tttgggcaga gtttctcaac cagaagatgt ttcaaatgta
     1981 gtgagcttct tagccggtaa agactctgat tacattactg gacaaacaat tattgtagat
     2041 ggtggtatga gattccgtta ataatcatcc actaatgata aataaatcct tattgttaag
     2101 tttaatcact tagcagtaag gattttttag tgcacttaga agggattgta ttggtagaaa
     2161 attaataaca gaagctctta agtgagttat gatgtcacag tctaatgcat catttgaaag
     2221 cattactaaa ttctatccag catagttacg ctaaaacatc ataaaacacc actatctaat
     2281 ttatccgcat aaaaattcac aaagttatct catttcattt ttataaataa aaaatatcga
     2341 taaaaagctt acaatacttt atgtttttgt gatatatttt taatgtataa atgaggtgga
     2401 agatttggaa aaagttttga taactggtgg ggcaggattt atagggtctc atttagtaga
     2461 ttttttgagc caagattatg atgtttatgt tctagataac tatagaacag gtaaacgaga
     2521 aaatattaaa agtttggctg acgatcatgt gtttgaatta gatattcgtg aatatgatgc
     2581 agttgaacaa atcatgaaga catatcaatt tgattatgtt attcatttag cagcattagt
     2641 tagtgtcgct gagtcggttg agaaacctat cttatctcaa gaaataaacg tcgtagcaac
     2701 gttgagatta ttagaaacca ttaaaaaata taacagtcat ataaaacgat tcatctttgc
     2761 atcatctgca gccgtttatg gtgatttacc tgatttacct aaaagtgatc aatcattaat
     2821 tctaccatta tcaccatatg caatagataa atattacggc gaacgtacga cattaaatta
     2881 ttgctcgcta tataacattc caacagctgt tgttaaattc tttaatgtat ttgggccaag
     2941 gcaagatcct aagtctcaat attcaggtgt gatttcaaaa atgttcgatt catttgaaca
     3001 taacaagcct tttacatttt ttggcgatgg tctgcaaacg agagactttg tttatgttta
     3061 tgatgttgtt caatctgtgc gtttaattat ggagcataaa gatgcggttg gacatggtta
     3121 taacattggt acgggtacat ttacgaattt gctagaggtt tatcgcatta ttggtgaatt
     3181 atatggaaaa tcagtcgagc atgattttaa agaagcaaga aagggagata ttaagcattc
     3241 ttatgcagat atttctaatt taaaagcatt aggatttgtt cctaaataca ctgtggaaac
     3301 aggcttaaag gattacttta attttgaggt agataatatt gaagaagtta cagcgaaaga
     3361 agtggaaatg tcgtgaaatt ggcattgaag ctgtccataa taagggttac gcctatcaaa
     3421 gaaaattaga cacactagaa gaagtaaaaa agggctattt tccaatcaaa cgtgcgattg
     3481 acttggtatt aagtatcgtt ttattatttt taacatttcc gattatgttc atattcgcca
     3541 ttgctatcgt catagattcg ccaggaaacc ctatttatag tcaggttaga gttgggaaga
     3601 tgggtaaatt aattaaaata tacaaattac gttcgatgtg taaaaacgca gagaaaaacg
     3661 gtgcgcaatg ggctgataaa gatgatgatc gtataacaaa tgtcgggaag tttattcgta
     3721 aaacacgcat tgatgaatta ccacaactaa ttaatgttgt taaaggggaa atgagtttta
     3781 ttggaccacg cccggaacgt ccagaatttg tagaattatt tagttcagaa ataataggat
     3841 ttgagcaaag atgtcttgtt acaccaggat taacaggtct tgcgcaaatt caaggtggat
     3901 atgacttaac accacaacaa aaattaaaat atgacatgaa atatatacat aaaggtagct
     3961 taatgatgga attatatata tcaattagaa cattgatggt tgttattaca ggggaaggct
     4021 caaggtagtc ttaatttgta tagaaaaatc aaatacaaga ttcattttca aaattgtgac
     4081 caattgttac agtataacga ggaatccctt gagacagtat caaatggcat taagaaatat
     4141 ttgccatcat tgatttgcat ggctataaat actattcatc tgatgagata gccatgttaa
     4201 gaaattgtaa gtaaagcatt aaaggggttt gtaacagttg aaaattatat attgtattac
     4261 taaagcagac aatggtggtg cacaaacaca tctcattcaa ctcgccaacc atttttgcat
     4321 acaccatgat gtttatgtca ttgtaggcaa tcatggacca atgattgaac aactagatgc
     4381 aagagttaat gtaattatta tcgaacattt agtaggtcca attgacttta aacaagatat
     4441 tttagctgtc aaagtgttag cacagttatt ctcgaaaatt aaacctgatg ttatccattt
     4501 acattcttct aaagctggaa cggtcggacg aattgcgaag ttcatttcga aatcgaaaga
     4561 cacacgtgta gtttttactg cgcatggatg ggcttttaca gagggtgtta aaccagctaa
     4621 aaaatttcta tatctagtta ttgaaaaatt aatgtcacgt attacagata gcattatttg
     4681 tgtttcagat ttcgataaac agttagcgtt aaaatatcga tttaatcgat tgaaattaac
     4741 cactatacat aatgggattg cagatgttcc cgctgttaag caaacgctaa aaagccaatc
     4801 acataacaat attggcgaag ttgttggaat gttgcctaat aaacaagatt tacagattaa
     4861 tgactcgaca aaacatcaat ttgttatgat tgcaagattt gcttatccaa aattgccaca
     4921 aaatctaatc gcggcaatag agatattgaa attacataac agtaatcatg cgcattttac
     4981 atttataggc gatggaccta cattaaatga ttgtcagcaa caagttgtac aagctgggtt
     5041 agaaaatgat gtcacatttt tgggcaatgt cattaatgcg agtcatttat tatcacaata
     5101 cgatacgttt attttaataa gtaagcatga aggtttgcca attagcatta tcgaagcgat
     5161 ggctacaggg ttgcctgtca tcgctagtca tgttggtggc atttcggaat tagtaactga
     5221 taatggtata tgtatagtga acaatcaacc tgaaactatt gccaaagtcc tggaaaaata
     5281 tttaatagac agtgattaca tcaaaatgag taaccaatct agaaaacgtt atttagaatg
     5341 ttttacagag gagaaaatga ttaaagaagt ggaagacgtt tataatggaa aatcaacaca
     5401 atagtaaata tctaacattg ttacttatcg gtttagcggt ttttattcag caatcttcgg
     5461 ttattgccgg tgtgaatgtt tctatagctg actttatcac attactaata ttagtttatt
     5521 tactgttttt cgctaaccat ttattaaagg caaatcattt tttacagttt ttcattattt
     5581 tgtatacata tcgtatgatt attacgttgt gtttactatt ttttgatgat ttgatattta
     5641 ttacggttaa ggaagttctt gcatctacag ttaaatatgc atttgtagtc atttatttct
     5701 atttagggat gatcatcttt aagttaggta atagcaaaaa agtgatcgtt acctcttata
     5761 ttataagcag tgtgactata ggtctatttt gtattatagc tggtttgaac aagtcccctt
     5821 tactaatgaa attattatac tttgatgaaa tacgttcaaa aggattaatg aatgacccta
     5881 actatttcgc aatgacacag attattacat tggtacttgc ttacaagtat attcataatt
     5941 acatattcaa ggtccttgca tgtggtattt tgctatggtc tttaactaca acggggtcta
     6001 agactgcgtt tatcatatta atcgtcttag ccatttattt ctttattaaa aagttattta
     6061 gtagaaatgc agtaagtgtt gtgagtatgt tagtgattat gttaattcta ctttgtttta
     6121 ccttttataa tataaactac tatttattcc aattaagcga ccttgatgcc ttaccgtcat
     6181 tagatcgaat ggcgtctatt tttgaagagg ggtttgcatc attaaatgat agtggatctg
     6241 agcgaagtgt tgtatggata aatgccattt cagtaattaa atatacatta ggttttggtg
     6301 ttggcttagt ggattatgta catattggct cgcaaattaa tggtatttta cttgttgccc
     6361 ataatacata tttgcaaatt tttgcggaat ggggcatttt attcggtgcg ttatttatca
     6421 tatttatgat ttatttatta tttgaattat ttagattcaa tatttctggg aaaaatgtaa
     6481 cagcaattgt tgtaatgttg acgatgctga tttacttttt aacagtatcg tttaataact
     6541 caagatatgt cgcttttatt ttaggaatta tcgtctttat tgttcaatat gaaaagatgg
     6601 aaagggatcg taatgaagag tgattcacta aaagaaaata ttatttatca agggctatac
     6661 caattgatta gaacgatgac accactgatt acaataccca ttatttcacg tgcatttggt
     6721 cccagtggtg tgggtattgt ttcattttct ttcaatatcg tgcaatactt tttgatgatt
     6781 gcaagtgttg gcgttcagtt atattttaat agagttatcg cgaagtccgt taacgacaaa
     6841 cggcaattgt ctcagcagtt ttgggatatc tttgtcagta aattattttt agcgttaaca
     6901 gtttttgcga tatatatggt cgtaattact atatttatag atgattacta tcttgttttc
     6961 ctactacaag gaatctatat tataggtgca gcactcgata tttcatggtt ttatgctgga
     7021 actgaaaagt ttaaaatacc tagccttagt aatattgttg cgtctgttat tgtattaagt
     7081 gtcgttgtta tctttgtcaa agatcaatca gatttatcgt tatatgtctt taccattgct
     7141 attgtgacgg tgttgaatca attacctttg tttatctatt taaaacgata cattagcttt
     7201 gtttcggtta attggataca cgtctggcaa ttgtttcgtt cgtcattggc atacttatta
     7261 ccaaatggac agctcaactt atatactagt atttcttgcg ttgttcttgg tttagtagga
     7321 acataccaac aagttggtat cttttctaac gcatttaata ttttaacggt cgcaatcata
     7381 atgattaata catttgatct tgtaatgatt ccgcgtatta ctaaaatgtc tatccagcaa
     7441 tcacatagtt taactaaaac gttagctaat aatatgaata ttcaattgat attaacaata
     7501 cctatggtct ttggtttaat tgcaattatg ccatcatttt atttatggtt ctttggtgag
     7561 gaattcgcat caa
//
LOCUS       DAPIDM010000122         7499 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000122 DAPIDM010000000
VERSION     DAPIDM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.122"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            278..1579
                     /locus_tag="RJE42_002155"
     CDS             278..1579
                     /locus_tag="RJE42_002155"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDV5877558.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGHGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAIDSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1593..1943
                     /locus_tag="RJE42_002156"
     CDS             1593..1943
                     /locus_tag="RJE42_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877559.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2095..2565)
                     /locus_tag="RJE42_002157"
     CDS             complement(2095..2565)
                     /locus_tag="RJE42_002157"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV5877560.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2874..4058
                     /locus_tag="RJE42_002158"
     CDS             2874..4058
                     /locus_tag="RJE42_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDV5877561.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIEQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKGQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4058..5251
                     /locus_tag="RJE42_002159"
     CDS             4058..5251
                     /locus_tag="RJE42_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDV5877562.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKDVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDTDLQKAITRIKDATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGCLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENECAFLA
                     ELERC"
     gene            5650..6321
                     /locus_tag="RJE42_002160"
     CDS             5650..6321
                     /locus_tag="RJE42_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDV5877563.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6451..7254)
                     /gene="coaW"
                     /locus_tag="RJE42_002161"
     CDS             complement(6451..7254)
                     /gene="coaW"
                     /locus_tag="RJE42_002161"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDV5877564.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLTEQGHALADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
BASE COUNT         2590 a         1057 c         1473 g         2379 t
ORIGIN      
        1 ttataaaaaa catgtaagca tgggacatcg taaccatata taagtatgtc gatatcagct
       61 ttaagacatc ataaatgcca tgatgattct aagataattt aatagggact ttgacgttat
      121 aaccaatcta tttaagaata ataacatcgc attgaaattg tgatattaaa aatgaaatcg
      181 atagcttgct ataataattt tcgcttgtgc ttttaaaaat ggactagaga aagcgcagat
      241 gctttgtcct aagtttttta agaaaaggat gataaaaatg agaatgatag acattattga
      301 gaaaaagcgt gacggtcata cactaacaac ggaagaaatt aatttcttta ttgacggcta
      361 tgttaaaggg gatattcctg attaccaagc atcaagttta gcaatggcga tttatttcca
      421 agatatgaat gatgatgagc gtgcagcatt aacgatggct atggttaatt ctggtgatat
      481 gatagatttg agtgatatta aaggtgtcaa agtagataag cactcaacag gtggtgtagg
      541 agatacaact acattggttt tagcaccatt agtagcagct gtagatgttc ctgttgcaaa
      601 aatgagtggg cgtggattag gtcatacagg tggcacaatt gataaattag aagcaattga
      661 tggttttcat gttgaaatag atgaagcaac atttgtgaaa ttggttaatg aaaataaagt
      721 ggcagttgta ggacaatcag gaaatttaac tcctgcagac aaaaaattat atgccttaag
      781 ggatgttact ggtaccgtca attcaatacc attaattgcc tcttcaatta tgagtaaaaa
      841 gattgccgct ggtgcagatg caattgtatt agatgtaaaa actggtagcg gtgcatttat
      901 gaaaacatta gaagatgctg aagcattagc gcacgcaatg gtacgtattg gtaataatgt
      961 gggacgtaat acgatggcga ttatttctga tatgaatcag ccacttggtc gtgcgattgg
     1021 caatgcgctt gagttgcaag aagcaattga tacgttgaaa ggacatggtc cgaaagattt
     1081 aacagaactt gtactaacat taggttctca aatggttgta cttgcaaaca aagctgaaac
     1141 gcttgaagaa gcgagagcat tgctaattga ggcaattgat tcaggtgcag cattagaaaa
     1201 attcaaaaca tttattaaaa accaaggtgg cgacgaaaca gttattgacc atccagagcg
     1261 tttgccacaa gctcaatatc aaatcgaata taaagctaaa aaatcaggtt atgtgactga
     1321 gttagtctct aacgatatag gtgtcgcttc gatgatgtta ggagcgggac gtttaacaaa
     1381 agaggatgat attgatttag cggttggtat tgttttaaat aaaaaaattg gggataaagt
     1441 agaagaagga gaatcattat taacgattca cagtaatcgc caagatgtag acgatgttgt
     1501 taaaaaatta gattcaagca taacaattgc tgatcatgtt gtatcgccta cattaattca
     1561 taaaattatt acagagtagg agttgaagct gattgaacat actaagatat ttattaggaa
     1621 taggttttag tgtaataggt gtattgcatt ttacacgaga acgacaattt agaaatatca
     1681 taccgaaatg tttgccactt cgaaaaacag cggttcttgt tacaggaatc tttgagatat
     1741 tatttggact tgcactttta attaaaagac catctcaatg cttgaaaaat acaatcaatc
     1801 tattcttgct agctgtattc ccagctaata tctatatggc agttaaaaag ttacctttag
     1861 gagataaaca attgccaaca tggcaactat atttaaggtt gcctatgcag ttcgtgttaa
     1921 tgggactaat taagaaatta tagtggttct taaataaatc ggacaaatgt taaagatttt
     1981 attttttcat ttgttcgatt ttttgatttt gaagcataat aaaaagcagt tggacaatgg
     2041 ctattgtaac atcattgttc gactgctttt ctgattaact tgattgacta agctttattt
     2101 tcctgtaccg aaaacatcat gccattcgtt tcgtttgtct agaaatgctt gagcgatagt
     2161 tttagcacct tctaatgaat gactagctgc ccagccacat tgtacttcat tacaagctgg
     2221 cacttcagta gcatttagca catcatttaa agttgcttca acaatattta atacatcatc
     2281 ataattatca tgattaataa atgatacata gaaaccagtt tggcaaccca taggacttaa
     2341 atcaacgact ttgtcactat gatttctaat attttcagcc attaaatgtt ctaatgaatg
     2401 caatcctggc atatccatat gttctttgtt tggttgtttg aaacgaatgt cgtatttgtg
     2461 aatgacatct ccgtttaatc cttccatcgt tcccgctaat ctaataaatg gggcaaccac
     2521 tttagtatga tctaaattga aactttcaac attcattttt gtcattttaa atttcctcct
     2581 attaactact caaaactata aaacgcgaat gattcattac attattaaac ttaaatttgt
     2641 tgcaagttgc gtttctaaca atacttaaat tgtaacaata ggacttatgt tttacaattt
     2701 gaaactttca taaaggtgga gcgtgatttt agttattaga aaattttgta aataaagaaa
     2761 aattagatga catcatgaga taaaaccgat agaataagta ataattcatt ttgaaagaac
     2821 aaaatattgt ggatgattaa aatgagtgta gggtaaaagg gagatagtaa aaaatgagtg
     2881 aaaaacaaca aattctcgat tatatagaaa caaataaata tagttatatc gaaatcagtc
     2941 atagaattca tgaacgtcct gaacttggta atgaagaaat atttgcgtct cgaactttaa
     3001 tagatcgttt gaaagaacat gattttgaaa ttgaaaccga gattgctggg catgcaacag
     3061 ggtttatagc gacatatgat tcggggcttg acgggccagc tataggtttt ttagcagaat
     3121 acgatgcttt gccaggatta ggccatgctt gtggtcataa tatcattgga actgcaagtg
     3181 tgcttggtgc aattggtttg aagcaagtga ttgaacaaat tggtggtaaa gtagtcgttc
     3241 tcggatgtcc agctgaagaa ggtggggaaa atggtagcgc taaagcttct tatgtcaaag
     3301 ctggtgtgat tgatcaaata gacattgcct taatgattca tccgggaaat gaaacttata
     3361 aaacgattga tactttggca gtggatgttt tagatgttaa attttacgga aaaagtgctc
     3421 atgcctctga aaatgcagat gaagcgttaa atgcattaga cgctatgatt agttatttta
     3481 atggtgtagc acaactacga caacatatta aaaaaggtca acgtgtgcat ggtgtgattt
     3541 tagatggcgg gaaagcagct aatattattc ctgactatac acatgctcgt ttttatacta
     3601 gagcaatgac gcgtaaagaa ttggatatat taacagaaaa agtaaatcaa atcgcacgtg
     3661 gagctgcgat acagactggt tgtgattatg aatttggtcc aattcaaaac ggtgtgaatg
     3721 aattcattaa aacgccgaaa ttagatgatt tatttgctaa atatgctgaa gaagttggtg
     3781 aagcagttat tgatgatgat tttggttatg gctctacgga tacagggaac gtaagtcatg
     3841 ttgtgccaac aatacatcct catattaaaa taggatcacg taatttagta ggacatacgc
     3901 atagatttag agaagcagct gcgagtgtac atggtgatga agcattaatt aaaggtgcta
     3961 aaataatggc gttgatgggg ttagagttaa ttacaaatca agacgtttat caagacatta
     4021 ttgaagagca tgcgcatttg aaagggaatg ggaagtaatg acaaataatg gtaatgagcc
     4081 gaacttaacg ttaagtgatt tatatgacaa agatgttgtc tatacgtcaa gaccttcgta
     4141 tatatcgaat ccatggttga aaccggacga acatcaatct aattttttaa ctggtaggga
     4201 gttattaata gcaaatcaat tacctgttat tgttcacgaa gctagtgcga cagacaaatt
     4261 gcatcagcta tttcaagtta ttggtaagga tgtaccgaac tcaatttata catttaacaa
     4321 tcagcaatct tacgaaaact tgattaaaca attggcacat aaggaaaata aaaagattta
     4381 ttttcaatat attcatgatg aaacaatctt gaatcagcaa tattatgcat tagataaaac
     4441 gttatttgtt gctttaaata ataaagcaag aatacctgaa tggacaaatg ggaagttttt
     4501 accaaaaaga aaagttgtaa aaattgagca attcgaaaac gagattaaga attgggaatt
     4561 cccgtttgta attaagccgg gagatgatct tccgacagca ggtggctatg gcgttatgat
     4621 ttgttatcat gatacagatt tacaaaaagc gataacgcgt attaaagatg caactgctga
     4681 aacgaattcg cttattatcg aacagaagat tgaagaaaaa gcgaattatt gtgttcagtt
     4741 tgcatactcg gaatcgctag gcattcaata tcttggtgcg gcaacgcaat taacggataa
     4801 gtacggattt tataacggaa atgaaaatac tactaatgta ccagaacacg ttatagaagc
     4861 aggtagacaa attatggaaa acggtgtgaa tcaaggattc tttggtgtgg ctggttttga
     4921 cttactcgtc gatgaggatg ataatgttta tgcgattgat ttaaacttta gacaaaatgg
     4981 ttcaacgagc atgttattac ttgctaacga gttgaattca ggatatcaaa agttttatag
     5041 ttatcattca aaaggtgata atacacattt cttcaatacg attttgaaat atgtcaaaga
     5101 aggttgttta tacccgttat cttattatga tggtgattgg tacggtgaag ataaagttaa
     5161 atcaaggttt ggctgtattt ggcatggaga ttcaaaagaa acagtactgg agaatgaatg
     5221 cgcattttta gctgaacttg aacgttgtta gagttcggaa cataaggcgc tacaatgtta
     5281 tgttgccagt agttgactga atatgcgctt gtagcaagct tttttcgatt ctagtcaaca
     5341 gtaattaaat ttatgatatg gcaatacttt gtaatactaa tattaaatgg cgactcttat
     5401 ttcactatgt tataagagtt gccattttgt tgataaaggc atattaaagg ttatcgttat
     5461 gaaattttta gtaactagat atgtttcgtg ttatagaccg aatttgtgta tacgtaaaat
     5521 ttaatgctat tgaattttta aaatgaaaaa catgacatta aattgaattc ataatatgtc
     5581 taattgacta tcttgttgga gtcatttact attttatgta tgacatattt taaaaagtga
     5641 gggtcaagca tgtcttataa agcatatcca ttctttagag atatattaat aaatgaatgt
     5701 atttatttcg cctctaaaaa taaaaaacta gtacgcctaa attataaaag tgaagcgtat
     5761 gtaggcgttt ggacagaaga aagtgtggcc gtatcatttt taacaagtcg tgatattcca
     5821 tttgataaag ttgtaaaaat ggacgttgat cgttttgcta cttatgaatt agatgaattg
     5881 tttgatgaac aagaccatat tattatgaat caaacaatgg aagaagaagg gcatctacta
     5941 aacgttgtag ctgttacaca agaagtgatg acggaattag ataaaattag aatcaaagaa
     6001 tttgtccaag atgtagcgaa atatgatgaa gtatacggct taactaaaaa aggtagtaag
     6061 cagtttattc tcattagtga aaatgatagc gacgaaaaaa agccgcatat tatgcctgta
     6121 tggagtatta aaaacagagc gttaaaagtt cgagatgaag attttgaaga gtgtgattta
     6181 attacgattg aaggttctgt tttcggagaa tggctagatg aacttagaga tgatcataaa
     6241 gccgttgcga tagatttaaa aactggcgtg gttggtacaa ttgtttcagc gcaaaaattg
     6301 tctaatgaac ttacatttta attgaattga gttgtaagta attaagtgat aataaatgga
     6361 acaatacgta ttcaaaacac gtagaccata aaataaaagg ccatttatat agcgtttatt
     6421 taaaacaacg ctcatataaa tggtcttttt ctattttcct aaatataatg caccaatagc
     6481 acctgaaaat gcgccgtttt caacatagta cggtttgcaa ccgcgtaaca cagtataatc
     6541 ttccacaact ttgcgtaata aagcgttatt atgaaatgaa gaaccgatat aaacgatatt
     6601 ttcagtttta aattctcgtg caacagtaat ggccattgtc gtaacaactt cgccaacgac
     6661 accaataacg gctgctaatt tattgctagg tgtaaaatca gcatctaaat gatgtagtac
     6721 atggccaaaa ttagctgctg ttaaatcacc gggaatgggt ggttcggtat ctttataaat
     6781 atgtctaacc tttaaatcga tagtgttacg atcaccgtgt tgtgccatgt cagttaactg
     6841 tttataatca gtgatttgac ttagtaaata accgagtcct tgaatcatgc ctccacctgt
     6901 accgataccg cctacacgac gttgtgattg gccgtcgaaa taatgtagtg acgtaccggt
     6961 accaacattt gcaaaaatat aatctgctaa ggcatggcct tgctctgtta acaaaatgcc
     7021 tagtccttga gatgcagcat caaactctac aaaaatttgt gcaggaatgt tgatgttttc
     7081 agcaatgaca cctgcattac ctccagttaa gcataatttt tcaatttgct gttggtttaa
     7141 ccattccaca acttgatcaa tatttttagt taattcagtt ttaaaagtac gttggttatc
     7201 ttgctcttga acgattttaa ttagtgtacc gccagcgtca atgccaactt tcataagatt
     7261 cccacctcat tattaatgtc tatccttaaa taatagtata gtaaaatgac taaaaaacaa
     7321 gtaataatag taattattaa caaatttgat gtcattgtat ttcaacattg taagcgtatc
     7381 gcaattaatg tattacaaac gtggacgtta cgttatatat attattttct aggaatttta
     7441 aagttgtata ggattgttag ttagtgacgt aatattaaaa gtagttcgta cgcagtgta
//
LOCUS       DAPIDM010000123         7374 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000123 DAPIDM010000000
VERSION     DAPIDM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.123"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            312..2297
                     /gene="uvrB"
                     /locus_tag="RJE42_002162"
     CDS             312..2297
                     /gene="uvrB"
                     /locus_tag="RJE42_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDV5877565.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            2305..5151
                     /gene="uvrA"
                     /locus_tag="RJE42_002163"
     CDS             2305..5151
                     /gene="uvrA"
                     /locus_tag="RJE42_002163"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV5877566.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNINLSE
                     QDQAIANQILKEIISRLAFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKINRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDN"
     gene            5761..6693
                     /locus_tag="RJE42_002164"
     CDS             5761..6693
                     /locus_tag="RJE42_002164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDV5877567.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            6699..>7374
                     /locus_tag="RJE42_002165"
     CDS             6699..>7374
                     /locus_tag="RJE42_002165"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV5877568.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLR"
BASE COUNT         2643 a         1032 c         1514 g         2185 t
ORIGIN      
        1 agatcaactc gaatttcata aacatcaaga tgaagatcgc taagattaac tttaaaataa
       61 tgtttcaaac aaatttgttg aaacaaaatg atgattaata taatgtgtat ttacatacta
      121 aaaataacaa gataaacgat ttgatttaag gcaagcatag ttagcacaac tatgtttgtt
      181 tttctttgtt cgacattttt acgaacaaac gtttgctttt tgtgtgacta ctttgctaaa
      241 atatgtaatg agaaatcgaa gagttgagcg gaaatataaa taatacattg aaagaggagg
      301 catatgtgac aatggttgaa cattatcctt ttaaaataca ttctgatttt gagcctcaag
      361 gtgaccaacc gcaagcaatt aaggaaatcg tggaaggtat taaagcgggg aaaagacatc
      421 aaactttatt aggtgcaact ggcacaggga aaacatttac gatgagtaat gttattaaag
      481 aagttggaaa accaacatta attatcgcgc ataacaaaac attagcagga caattatata
      541 gtgagtttaa agaatttttt cctgaaaaca gggtggaata ctttgtaagt tactatgatt
      601 attatcaacc tgaggcatac gtaccgtcta ctgacacttt tattgaaaaa gatgcctcaa
      661 tcaatgatga aattgatcaa ctacgacatt ctgctacaag tgcattattt gaacgcgatg
      721 atgtaattat tattgctagt gtaagttgta tatatggttt aggtaatcct gaagaatata
      781 aagatttagt agtaagtgtt cgagttggta tggaaatgga tagaagcgaa ttacttagaa
      841 aacttgtaga tgtgcaatat acacgaaatg acatcgattt ccaacgagga acgtttcgag
      901 tgcgtggtga tgtagttgaa atattcccag cctctaaaga agaactttgt ataagggttg
      961 agtttttcgg tgatgagatt gaccgaattc gagaagttaa ctacctaaca ggtgaagtgt
     1021 tgaaagaaag agaacatttt gcgatattcc cagcttctca cttcgtaaca cgtgaagaaa
     1081 agttgaaagt tgcgattgaa cgtattgaaa aagaattgga agaacgattg aaagaattac
     1141 gagatgagaa taaattacta gaagcgcaaa ggttagaaca gcgtaccaac tatgatttag
     1201 aaatgatgcg agagatggga ttctgttcag gaattgaaaa ctattccgta catttaactt
     1261 tgcgaccact gggttcgaca ccatatactt tattggatta ctttggcgat gattggttag
     1321 taatgattga tgaatcacat gtgacattac cgcaagttcg aggcatgtat aacggagaca
     1381 gagcgcgtaa acaagttttg gtggatcatg gatttagatt gccgagtgca ttagataacc
     1441 gtccacttaa atttgaagaa tttgaagaaa agacaaaaca acttgtgtat gtatctgcaa
     1501 cgcctggacc atacgaaatt gaacatacgg ataagatggt tgaacaaatt attcgtccta
     1561 ctggtttact ggatcctaag attgaggtta gacctactga aaatcaaatt gacgatttat
     1621 taagtgaaat tcaaacaagg gttgagcgta atgaacgcgt acttgttaca acgctcacta
     1681 aaaagatgag tgaagattta accacataca tgaaagaagc gggtattaaa gttaattatc
     1741 tgcattcaga aatcaagaca ttagaacgaa tcgaaataat tagagactta cgaatgggta
     1801 catatgatgt tatcgtaggt attaatttat taagagaggg tattgatata ccagaagttt
     1861 ctctagttgt catattagat gcagataaag aaggattttt acgttctaac cgctcattaa
     1921 ttcaaacaat aggtagagct gcgcgtaacg ataaaggtga agtcattatg tatgccgata
     1981 aaatgactga ttcgatgaag tatgcaattg atgagacaca acgtcgtcga gaaatacaga
     2041 tgaaacataa tgaaaaacat ggtattacac ctaaaacaat taataaaaaa atacatgatt
     2101 taattagtgc tactgttgaa aatgacgaaa ataatgacaa agcacaaact gtgataccta
     2161 agaagatgac gaaaaaagaa cgtcaaaaga caatcgacaa tatagaaaaa gaaatgaaac
     2221 aagcagcgaa agatttagat ttcgagaaag ctacagaatt aagagatatg ttatttgaat
     2281 taaaagcaga agggtgacaa gtaaatgaaa gaaccatcca tagtagtaaa aggtgctcgt
     2341 gcgcataact tgaaagatat tgatatcgaa ctacctaaaa ataaattaat tgttatgaca
     2401 ggattatctg ggtcaggtaa atcgtcatta gcattcgata ctatatatgc tgaaggacaa
     2461 cgtcgttatg ttgaatcatt aagtgcctat gcgcgtcaat ttttaggtca aatggacaaa
     2521 ccagatgttg atacaattga aggattatcg ccagcaattt caatagatca aaaaacaaca
     2581 agcaaaaatc caaggtcaac tgttgcaaca gtaacagaaa tatatgatta tatacgtttg
     2641 ttatatgcac gtgttggtaa accttactgt ccaaatcaca atatagaaat tgaatcgcaa
     2701 acagtacaac aaatggttga ccgcattatg gaattagagg cacgtacaaa gattcaatta
     2761 ttagcacctg tcatcgctca tcgtaaaggt agtcatgaaa agctaatcga agatattggt
     2821 aaaaaaggtt atgtacgttt aagaatcgat ggcgaaattg ttgatgtaaa tgatgtacct
     2881 acattagata agaacaagaa tcatacaata gaagttgttg tagaccgatt agttgttaaa
     2941 gatggaattg aaacacgact agctgactct atagaaactg cgttagagct ttcagaagga
     3001 caattaacag tcgatgtcat tgacggggaa gaccttaagt tttcagaaag ccacgcttgt
     3061 cctatatgtg gattttcaat cggagagtta gaaccaagaa tgtttagctt taacagtcct
     3121 tttggtgctt gtccaacttg tgatggctta ggtcaaaagt taacagtcga tgtagacttg
     3181 gttgttccag acaaagataa gacgctaaac gaaggtgcaa tagaaccatg gataccgacg
     3241 agttctgatt tttatccaac attgttaaaa cgtgtttgtg aagtttataa aatcaatatg
     3301 gataaacctt ttaaaaagtt aacagaacgt caacgtgata ttttattgta tggttcgggt
     3361 gacaaagaaa ttgaatttac atttacacaa cgtcaaggtg gtactagaaa acgaacaatg
     3421 gttttcgagg gggtagttcc taatataagt agacgattcc atgaatctcc ttcagaatat
     3481 acacgtgaaa tgatgagtaa atatatgacc gaactacctt gcgaaacttg tcatggaaag
     3541 cgattgagtc gtgaagcgtt atctgtttat gtaggtggtt taaatattgg tgaagtagtc
     3601 gaatactcaa ttagtcaagc gctgaactat tataaaaaca ttaatttgtc agaacaagat
     3661 caagcgattg caaatcaaat attgaaagag attatttccc gactcgcttt tttaaataat
     3721 gtgggacttg aatatttaac actaaacaga gcttcaggta cactttcagg tggtgaagca
     3781 caacgtattc gattggcaac gcaaattggg tcgcgtttga ctggtgtctt atatgtatta
     3841 gatgagccat caattggact gcatcaaaga gataatgatc gattaattaa tacacttaaa
     3901 gaaatgagag atttaggaaa tactttaatt gtagttgaac acgatgatga tacaatgcgt
     3961 gcggctgatt acttagtgga catagggcct ggtgctggtg agcatggagg acagattgtg
     4021 tctagtggta cacctcaaaa ggtaatgaaa gataaaaaat ctttaacagg acaatacttg
     4081 agtggtaaga aacgtattga agtacctgaa tatcgcagac cggcttcaga tcgtaaaatt
     4141 tctatacgtg gagctagaag taacaacctt aaaggggttg atgtggacat accactatca
     4201 atcatgacgg tcgttacagg tgtatcaggt tcaggtaaaa gctcattagt aaatgaagta
     4261 ttatacaaat cattagctca aaaaatcaat aaatctaaag taaagccagg attgtacgat
     4321 aagattgaag gtattgatca acttgataaa attattgata ttgatcaatc accgataggt
     4381 agaacgccac gctctaatcc agcaacgtat actggtgtgt ttgatgatat acgtgatgtg
     4441 tttgcgcaaa caaatgaagc taaaattcga ggatatcaaa aaggccgttt tagttttaat
     4501 gtaaaaggtg gacgctgtga agcttgtaaa ggtgacggta ttattaaaat tgaaatgcat
     4561 tttttacctg atgtttatgt tccttgtgaa gtgtgtgatg gtaaacgata taatcgtgag
     4621 acactagagg ttacttacaa aggtaaaaat attgctgaca ttttagaaat gactgttgaa
     4681 gaagcaacac aattttttga aaatattcct aagattaatc gcaagttaca aacactagtt
     4741 gatgttggtc ttggatacgt cacattaggt caacaagcta caacgttatc aggtggtgag
     4801 gctcaacgtg tgaaacttgc atctgaactt cataaacgtt caactggtaa atctatttat
     4861 atcctagatg aaccgacaac agggttacat gttgacgata ttagtagatt attaaaagta
     4921 ttaaaccgat tagttgaaaa tggtgacact gttgtaatta ttgaacataa cctagatgtt
     4981 atcaaaacag cagactatat tatagactta ggtcctgaag gtggtagtgg cggtggtact
     5041 attgttgcga ctggcacacc cgaagatatt gctcagacaa agtcatcata tacaggaaag
     5101 tatttaaaag aagtacttga acgagataaa caaaatactg aagataatta agattaaaag
     5161 aagtgaagga tgttgtaaat ttatccttcg cttcttttta ttaatttagt aatgaatagt
     5221 agaaagaaaa gatgcgtaaa aagaattatg ttaagatagg atcgatctag agtagctaaa
     5281 cataaatcga aactgggagc gggacagaaa tgataaagaa tcactaatga tttattatgt
     5341 attggttctt tgtcattagc cacagctatt gtgtacttaa aaataggaat gcatgagtaa
     5401 aactcataca taagaaatac taatttctaa agaaaaagta tttctttatg ttgggtccca
     5461 ccccaacttg cattgtctgt cgaaattgag aatccaattt ctctgtgttg gctccccgcc
     5521 aactaattcg aatttatcat tttagagctt aggacattga tttatggctt ggacttttat
     5581 gtcgatatga accatgtaaa ttaagcaagc aataaattaa tgattgatat tgacttgtaa
     5641 aataataaca ataatgaaca attaatattt attttagctt ttcaatgtag attggtgtta
     5701 tatttttgat atgataagaa gagatgtaag agtagggata aatacaattg aggtgaaccc
     5761 atgttaacga cagaaaaact agttgaaaca ttaaagttag atttaatcgc tggtgaagaa
     5821 ggactatcga agccaattaa aaatgctgat atatcaagac cgggcttaga gatggcaggt
     5881 tatttttcac attatgcgtc agatagaata caactattag gaacaacgga actatcgttt
     5941 tacaatttat taccagataa ggatcgcgca ggtcgtatgc gtaaactatg cagaccagaa
     6001 acgcctgcaa ttattgtgac acgtggattg cagccaccag aagaattagt tgaagctgca
     6061 aaagaattaa atacgccact tatagttgct aaagatgcca ctacaagttt aatgagtcgc
     6121 ttaacaacgt tcttagagca tgcacttgca aagacgacat ctttacatgg tgttttagta
     6181 gatgtttacg gtgttggtgt actaattacc ggtgattcag gaataggtaa aagtgagact
     6241 gcgttggagt tagttaaacg tgggcataga ttagtagcag atgataatgt agaaatacgt
     6301 caaattaata aagatgaact aatagggaaa ccaccaaagt taatagaaca tctattagaa
     6361 atacgtggac taggtattat caatgttatg actttatttg gcgcgggttc aatattaact
     6421 gaaaaacgaa ttagattaaa tattaatttg gaaaactgga acaagcaaaa gttatatgac
     6481 cgcgtaggtc ttaatgaaga gacgttaagt attttagata ctgaaataac taaaaaaaca
     6541 atacctgtaa gacctggtag aaatgttgcg gtaattattg aggtcgctgc aatgaactat
     6601 cgattaaata tcatgggcat taacactgcc gaagaattta gtgaaagatt aaatgaagaa
     6661 attatcaaga acagtcataa gagtgaggag taggttgaat gggtattgta tttaactata
     6721 tagatcctgt ggcatttaac ttaggaccac tgagtgtacg atggtatgga attatcattg
     6781 ctgtcggaat actacttggt tactttgttg cacaacgtgc actagttaaa gcaggattac
     6841 ataaagatac tttagtagat attatttttt atagtgcact atttggattt atcgcggcac
     6901 gaatctattt cgtgattttc caatggccat attacgcgga aaatccaggt gaaattatta
     6961 aaatatggca tggtggtata gcaatacatg gtggtttaat aggtggcttt attgctggtg
     7021 ttattgtatg taaagtgaaa aatttaaacc catttcaaat tggtgatatc gttgcgccaa
     7081 gtataatttt agcgcaagga attggacgct ggggtaactt tatgaatcac gaggcacatg
     7141 gtggacctgt gtcacgcgct tttttagaac aattacattt gcctaatttt ataatagaaa
     7201 atatgtatat taacggccaa tattatcatc caacattctt atatgaatcc atttgggatg
     7261 tcgctggatt tattatccta gttaatattc gtaaacattt aaaattagga gaaacattct
     7321 ttttatattt aacttggtat tcaattggtc gattctttat agaaggatta cgta
//
LOCUS       DAPIDM010000124         7361 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000124 DAPIDM010000000
VERSION     DAPIDM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.124"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..178)
                     /locus_tag="RJE42_002166"
     CDS             complement(<1..178)
                     /locus_tag="RJE42_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDV5877569.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILY"
     gene            232..603
                     /locus_tag="RJE42_002167"
                     /pseudo
     CDS             232..603
                     /locus_tag="RJE42_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            1520..2263
                     /locus_tag="RJE42_002168"
     CDS             1520..2263
                     /locus_tag="RJE42_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDV5877570.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2360..2788
                     /locus_tag="RJE42_002169"
     CDS             2360..2788
                     /locus_tag="RJE42_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDV5877571.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2834..4843)
                     /gene="mecA"
                     /locus_tag="RJE42_002170"
     CDS             complement(2834..4843)
                     /gene="mecA"
                     /locus_tag="RJE42_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDV5877572.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLRDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            4940..5047
                     /locus_tag="RJE42_002171"
                     /pseudo
     CDS             4940..5047
                     /locus_tag="RJE42_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
     gene            5088..5762
                     /locus_tag="RJE42_002172"
                     /pseudo
     CDS             5088..5762
                     /locus_tag="RJE42_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(5822..6250)
                     /locus_tag="RJE42_002173"
     CDS             complement(5822..6250)
                     /locus_tag="RJE42_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV5877573.1"
                     /translation="MKFQVSPYFLITQNAEGLKKLYEELFGAKTLYIQRIKDRPIDKK
                     LNIDENDLNKIDQCVLQFGDIKIMIADDTEGLPITQGNNISLCLTFDQVEETKHVYDQ
                     LIEKGSEVLKTFSPEFYTEGYGYVRDPYGISLHLFTKRKN"
     gene            6331..7260
                     /locus_tag="RJE42_002174"
     CDS             6331..7260
                     /locus_tag="RJE42_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDV5877574.1"
                     /translation="MKKSVRLYNMIEYCNENRNFKLNDLMSEFNISRSTALRDIKEIE
                     ALGVPLYSNPGKNGGYTIIGNRDQTKIAISDEELKALVFTLSSISNVSNLPFQTEYQE
                     ILKKLYNNSNKKELINQYNDLFQYFNEDKYQFKSYKLFNEIIRLIIENKSFETCYSQN
                     YIKEQYKGIGIMYKNHQWYFVVANIESKLVNLLNISKIKELYEMGETQECNDITMQNF
                     QQFMVKNETAIDILIRSNVMGLNILKGYLWSDYMIENIDEETYLFKSKVNAKDIDFIA
                     KLIVTGGVNVKVETPNSLKNAVKVELTKIINMY"
BASE COUNT         2594 a         1051 c         1189 g         2527 t
ORIGIN      
        1 gatacaaaat tggggcatat tcttgaaccc aacggtagac cgttgaatga tgaacgttta
       61 caccacgttc ccttaatatt tcagatatat cacgataact caatgtatat cttagatagt
      121 agccaacggc tacagtgata acatccttgt taaattgttt atatctgaaa tagttcatac
      181 agaagactcc tttttgttaa aattatacta taaattcaac tttgcaacag aaccgtatta
      241 tggaatagag atgttggtaa catttataca ggatcattat acttaagttt aatttcgtta
      301 ttacagaacc acacattcca accagaagag aaagtatgtc tatttagtta tggttcagga
      361 gcagtaggag aaatctttag tggttcaatc gttaaaggat atgacaaagc attagataaa
      421 gagaaacact taaatatgct agaatctaga gagcaattat cagtcgaaga atacgaaaca
      481 ttctttaaca gatttgataa tcaagaattt gatttcgaac gtgaattgac acaagatcca
      541 tattcaaaag tatacttata cagtatagaa gaccatatca gaacatataa gatagagaaa
      601 taaactagtg gccgattgtg cttgatgagc ttgggacata aatcctaact cgaaataaat
      661 aagcatatca ctaaactgat tttttaaagt ttacagtgat atgcttattt ttttatctta
      721 cgattttgta cgtgcatgct tgcctagggg tatggctcga gccattagtc tctcgcacat
      781 actattccct caggcgtcag cacttacaaa atcggttgta attttcattt ttatacgcat
      841 tcttactgag attatactaa taagaggaat agtaaaagca attctaagta aaattgcaga
      901 taagaggttt gttaaaagca gttctaagta aaattgcaga taagaggttt gttaaaagca
      961 gttctcagta aaattacaga taagaggtac gttaaaagca gttctaagta aaattgcaga
     1021 taagaggttt gttaaaagca gttctaagta aaattgcaga taagaggtac gttaaaagca
     1081 attccatgca aaattgctga taaggggtaa gttaaaagca gttctcagta aaattgcaga
     1141 taagaggtac gttaaaagca gttctaggca aaattgcaga taagaggtgc gttaaaagca
     1201 gttctcagta aaattgctga taaggggtaa gttaaaagca atcctaagta aaattgcaga
     1261 taagaggtaa gttaaaagca atcctaagta aaattgcaga taaggggtac agaaaaacta
     1321 gacttgatta caaaatggag cttgggacat aaatgatttt ttaaaaatga gatgagacgt
     1381 agattaactc cataatcaat acgaatctat cgacttcttt atttatgata ttcatctctt
     1441 tttaatggaa ataaaagtgc gattaatgtg ataatacagt tacgttaatt aaaaaaataa
     1501 aaatgcaagg agaggtaata tgctaactgt atatggacat agaggattac ctagtaaagc
     1561 tccggaaaat acaattgcat catttaaagc tgcttcagaa gtagaaggta taaactggtt
     1621 ggagttagat gttgcaatta caaaagatga acaactgatt atcattcatg atgattattt
     1681 agaacggact acaaatatgt ccggggaaat aactgaattg aattatgatg aaattaaaga
     1741 tgcttctgca ggatcttggt ttggtgaaaa attcaaagat gaacatttgc caactttcga
     1801 tgatgtagta aaaatagcaa atgaatataa tatgaattta aatgtagaat taaaaggtat
     1861 tactggaccg aatggactag cactttctaa aagtatggtt aagcaagtgg aagaacaatt
     1921 aacaaactta aatcagaatc aagaagtgct catttcaagc tttaatgttg tgcttgttaa
     1981 acttgcagaa gaaatcatgc cacaatataa cagagcagtt atattccata caacttcgtt
     2041 tcgtgaagac tggagaacac ttttagatta ctgtaatgct aaaatagtaa acactgaaga
     2101 tgccaaactt actaaagcaa aagtaaaaat ggtaaaagaa gcgggttatg aattgaacgt
     2161 atggactgta aacaaaccag cacgtgcaaa ccaacttgct aattggggag ttgatggtat
     2221 ctttacagac aatgcagata aaatggtgca tttgtctcaa tagaaagtta gaggtgagtc
     2281 ttacgtttca gtgacggtag acttaccttt aacatgttac atactaaaaa attaatttga
     2341 ataagaaaga gagacatata tgaaatacga tgattttata gtaggagaaa cattcaaaac
     2401 aaaaagcctt catattacag aagaagaaat tatccaattt gcaacaactt ttgatcctca
     2461 atatatgcat atagataaag aaaaagcaga acaaagtaga tttaaaggta tcattgcatc
     2521 tggcatgcat acactttcaa tatcatttaa attatgggta gaagaaggta aatacggaga
     2581 agaagttgta gcaggaacac aaatgaataa cgttaaattt attaaacctg tatacccagg
     2641 taatacattg tacgttatcg ctgaaattac aaataagaaa tccataaaaa aagaaaatgg
     2701 actcgttaca gtgtcacttt caacatacaa tgaaaatgaa gaaattgtat ttaagggaga
     2761 agtaacagca cttattaata attcataata aaacagtgaa gcaaccatcg ttacggattg
     2821 cttcactgtt ttgttattca tctatatcgt attttttatt accgttctca tatagctcat
     2881 catacacttt acctgagatt ttggcattgt agctagccat tcctttatct tgtacatctt
     2941 taacattaat agccatcatc atgtttggat tatctttatc atatgatata aaccacccaa
     3001 tttgtctgcc agtttctcct tgtttcattt tgagttctgc agtaccggat ttgccaatta
     3061 agtttgcata agatctataa atatcttctt tatgtgtttt atttacgact tgttgcatac
     3121 catcagttaa tagattgata ttttctttgg aaataatatt tttcttccaa actttgtttt
     3181 tcgtgtcttt taataagtga ggtgcgttaa tattgccatt attttctaat gcgctataga
     3241 ttgaaaggat ctgtactggg ttaatcagta tttcaccttg tccgtaacct gaatcagcta
     3301 ataatatttc attatctaaa tttttgtttg aaatttgagc attataaaat ggataatcac
     3361 ttggtatatc ttcaccaaca cctagttttt tcatgccttt ttcaaatttc ttactgccta
     3421 attcgagtgc tactctagca aagaaaatgt tatctgatga ttctattgct tgttttaagt
     3481 cgatattacc atttaccact tcatatcttg taacgttgta accaccccaa gatttatctt
     3541 tttgccaacc tttaccatcg attttataac ttgttttatc gtctaatgtt ttgttattta
     3601 acccaatcat tgctgttaat attttttgag ttgaacctgg tgaagttgta atctggaact
     3661 tgttgagcag aggttctttt ttatcttcgg ttaatttatt atattcttcg ttactcatgc
     3721 catacataaa tggatagacg tcatatgaag gtgtgcttac aagtgctaat aattcacctg
     3781 tttgagggtg gatagcagta cctgagccat aatcattttt catgttgtta taaatactct
     3841 tttgaacttt agcatcaata gttagttgaa tatctttgcc atcttttttc tttttctcta
     3901 ttaatgtatg tgcgattgta ttgctattat cgtcaacgat tgtgacacga tagccatctt
     3961 catgttggag ctttttatcg taaagttttt cgagtccctt tttaccaata actgcatcat
     4021 ctttatagcc tttatattct ttttgtttta attcttcaga gttaatggga ccaacataac
     4081 ctaatagatg tgaagtcgct tttcctagag gatagttacg actttctgtt tcattagttg
     4141 taagatgaaa tttttttgcg aaatctctta aatattcatc cattttttta acggttttaa
     4201 gtggaacgaa ggtatcatct tgtacccaat tttgatccat ttgttgtttg atatagtctt
     4261 cagaaatact tagttcttta gcgattgctt tataatcttt tttagataca ttctttggaa
     4321 cgatgcctat ctcatatgct gttcctgtat tggccaattc cacattgttt cggtctaaaa
     4381 ttttaccacg ttctgatttt aaattttcaa tatgtatgct ttggtctttc tgcattcctg
     4441 gaataatgac gctatgatcc caatctaact tccacatacc atcttcttta acaaaattaa
     4501 attgaacgtt gcgatcaatg ttaccgtagt ttgttttaat tttatattga gcatctactc
     4561 gttttttatt tttagatact ttttttattt tacgatcctg aatgtttata tctttaacgc
     4621 ctaaactatt atatattttt atcggacgtt cagtcatttc tacttcacca ttatcgcttt
     4681 tagaaatata actgctatct ttataaactt gtttgaaatt tttatcttca attgcatcaa
     4741 tagtattatt aatttcttta tcttttgaag cataaaaata tataccaaac ccgacaacta
     4801 caactattaa aataagtgga acaattttta tctttttcat caatatcctc cttatataag
     4861 actacatttg taatatatta caaatgtagt atttatgtca aaataatgtt ataatttttg
     4921 tgatatggag gtgtagaagg tgttatcatc ttttttaatg ttaagtataa tcagttcatt
     4981 gctcacgata tgtgtaattt ttttagtgag aatgctctat ataaaatata cggttctgtt
     5041 gcaaagttga atttatagta taattataac caaaaggagt cttctgtatg aactatttca
     5101 gatataaaca atttaacaag gatgttatca ctgtagccgt tggctactat ctaagatatg
     5161 cattgagtta tcgtgatatg tctgaaatat taagggaacg tggtgtaaac gttcatcatt
     5221 taacggtcta gcgttgagtt caagaatatg ccccgatttt atatcaaatt tggaagaaaa
     5281 agcataaaaa agcttattac aaatggcgta ttgatgagac gtacatcaaa ataaaaggaa
     5341 aatggaacta tttatatcgt gccattgata cagagggaca tacattagat atttggttgc
     5401 gtaagcaacg agataatcat tcagcatatg tatttatcaa acgtctcatt aaacaatttg
     5461 gtaaacctca aaaggtaatt acagattagg caccttcaac gaaggtcgca atggctaaag
     5521 tcattaaagc ttttaaactt aaacctgact gtcattgtac atcgaaatat ctgaataacc
     5581 tcattgagca agatcaccgt catattaaag taagaaagac aagatatcaa agtatcaata
     5641 cggcaaagaa tactttaaaa ggtattgaat gtatttacgg tctatataaa aagaaccgca
     5701 ggtctcttca gatctgcgga ttttcgccat gccatgaaat tagcatcatg ctaacaagtt
     5761 aacacaaagt attattttaa aattgagatt agacatttat ttttcaactt tgcagcagaa
     5821 cctaattttt tctctttgta aataaatgca aagagatacc ataaggatct cttacataac
     5881 cataaccttc agtatagaat tctggactaa atgttttcaa tacctcactg cctttttcta
     5941 ttaactggtc atatacatgt ttagtttctt ctacttggtc aaaagtgaga caaagagata
     6001 tattattacc ttgtgttatg ggcaaacctt cagtgtcatc tgcgatcata atttttatat
     6061 ctccaaattg aagtacacat tgatcaattt tatttaaatc attttcgtca atattaagtt
     6121 tcttatctat aggtctatct ttaatacgtt gaatatacag tgttttagcg ccaaacagct
     6181 cttcatacaa cttttttaaa ccctctgcat tttgagtgat taaaaaatat ggacttactt
     6241 gaaatttcat gttttttcct ccttagattt gttataaata gagtatatac ttaattagtg
     6301 tcatctattg acactaaaag gagaataata atgaaaaaat ctgttagatt atataatatg
     6361 attgaatatt gtaatgaaaa taggaacttc aaattaaatg atttaatgtc agaatttaat
     6421 atttctcgta gtaccgcttt aagggatata aaagaaattg aagcattagg agtaccttta
     6481 tatagtaatc cagggaaaaa tggtggttat acgatcatag gtaatcgaga ccaaacgaaa
     6541 atagcaatct cagatgaaga gttgaaagct ttagtattta cactttcgag tatttcaaat
     6601 gtgagtaatc taccttttca aacagaatat caagaaatat taaaaaaatt atataataac
     6661 tcgaataaaa aagagttaat aaaccaatat aatgatctat ttcaatattt taatgaagat
     6721 aagtatcagt tcaaaagtta taagttattt aatgaaatca ttagattgat aattgagaat
     6781 aagtcttttg aaacctgtta ttcacaaaac tatattaaag aacaatataa aggtattggc
     6841 attatgtata aaaatcatca atggtatttt gttgtagcta atatagaatc aaagttagtg
     6901 aatctattaa atatttcgaa aataaaagaa ctatatgaaa tgggagagac tcaagagtgt
     6961 aatgatataa ctatgcagaa ttttcaacag ttcatggtta aaaatgaaac agctattgat
     7021 attcttatta gaagcaatgt tatgggattg aatatcttga aaggctacct gtggagtgac
     7081 tatatgattg aaaatattga cgaagagaca tatttattta aatcaaaagt gaacgcgaaa
     7141 gatatagatt ttatagctaa gttaattgtc acaggtggtg tcaatgtaaa agtagagacc
     7201 cctaatagtt tgaaaaatgc tgttaaagtt gaattaacta aaataataaa catgtattaa
     7261 tagtaattta atcataattg taataacaaa aagatgacaa cattaaacaa tttaaattta
     7321 tacaaaaaag cattaccaaa tttaaaattg ggtagtggta g
//
LOCUS       DAPIDM010000125         7242 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000125 DAPIDM010000000
VERSION     DAPIDM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.125"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(433..1362)
                     /locus_tag="RJE42_002175"
     CDS             complement(433..1362)
                     /locus_tag="RJE42_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV5877575.1"
                     /translation="MLLFYIIGVIVGMFIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIVLNIFINPDVFSLHFFSNQTFNYTWFVGGLLGVGYLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIITMNQINKCNILLTNQKH
                     LIFWLLLGFTFGFFPPIQTTINSLLASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1555..1779)
                     /locus_tag="RJE42_002176"
     CDS             complement(1555..1779)
                     /locus_tag="RJE42_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDV5877576.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNIVTEQVARDSMNALVDLN
                     ILKSNSGAITKVHSAQIIDKTTTVLFEAKK"
     gene            complement(1796..1999)
                     /locus_tag="RJE42_002177"
     CDS             complement(1796..1999)
                     /locus_tag="RJE42_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877577.1"
                     /translation="MSKTNHITIVLSFTKLDTNGKQTEFKRRFANINPEVSNDQIKTF
                     SKLIERLTGETYNNIEVIKSESI"
     gene            2154..2714
                     /locus_tag="RJE42_002178"
     CDS             2154..2714
                     /locus_tag="RJE42_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV5877578.1"
                     /translation="MNKVEVIKCNDDINKMYDALKARSDRDYLFFKLAIHSGMKITEL
                     LTITVEDTKRLIEKGTLSELCKSHYHSLIKIRLPETLSNELLHYIENKSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQAAIKAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            2733..5135
                     /gene="mnhA2"
                     /locus_tag="RJE42_002179"
     CDS             2733..5135
                     /gene="mnhA2"
                     /locus_tag="RJE42_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDV5877579.1"
                     /translation="MSLVYLLIAILVIMAMILFTSKRRAMAKYAGYIALTAPVIASIY
                     FLLQVPSVIKQHYLSVSIPWMTSLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYLYLTLFMFSMLGIVLADNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHALFIPMIIMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFAALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGIPFLNGFLSKEMFLDALTQTGQLSQFSLISMIIIVCMGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLVPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRQFEKFATKRFKRVMQDRLNQYIIMTLGIFMVIIGYGYIRIGLPKVHQLHVS
                     EFGPLEVILAIVTVIIGLSLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSKVNKKREIIKISVSLMMALIVVSLIFIAQQADGLAS
                     ISNFYLRADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            5122..5547
                     /gene="mnhB2"
                     /locus_tag="RJE42_002180"
     CDS             5122..5547
                     /gene="mnhB2"
                     /locus_tag="RJE42_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDV5877580.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIMPTFFGKPFLSQYETTLT
                     LPILGHIHVTTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5544..5888
                     /gene="mnhC2"
                     /locus_tag="RJE42_002181"
     CDS             5544..5888
                     /gene="mnhC2"
                     /locus_tag="RJE42_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDV5877581.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSNKSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5878..>7242
                     /gene="mnhD2"
                     /locus_tag="RJE42_002182"
     CDS             5878..>7242
                     /gene="mnhD2"
                     /locus_tag="RJE42_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDV5877582.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWTAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPFFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLTLMIITSLIAMYSLFRILFYMYFGDKDGEEVHFKRIPQYRKG
                     ILSIL"
BASE COUNT         2382 a         1031 c         1252 g         2577 t
ORIGIN      
        1 cactttttta atgacaaaag taaaactata gcactttaaa gtaaatgtat agtaatccga
       61 ttgataacaa gtaatcaata ttagaaagta cgattatggt tcataattta aagcatcttc
      121 aatctttaaa gtcgactcaa tgaatgaatt aaagttgtac attttgactt atttttaatt
      181 aattgaataa ccaactatga aatattagca cgaataacat aatttttgct attaatttgg
      241 tgtaaattat taaaaaataa cattaattta ggtcttaaga atgagttgac tatcgaattt
      301 gctaaattaa aatttgttgt gtcaatgcat actacccata ttagtactta ttaaaactaa
      361 tttagactgc aaaaaaatag cttatcactt ttaaaaataa ttacaagtta aaatgataag
      421 ctaaaagtta atttaaaata atcttaaaag tataatgcct gtcaaaatac ataatagacc
      481 aatagttttt ctggatgtca ttgctatttt aggtgaacca aataatccaa agtgatctat
      541 caatatgccc attagaatct ggccaaacat cccaataagt gttgttaatg ctgcacccat
      601 atgaggcatt aagataatgt tagctgttac aaaagccatg ccaagtatac cgccagtaaa
      661 atagataggt tttaatttac cgaattttaa atgacttgtt tttagtttta aagaacgatt
      721 aaaaatagcg gttaaaatca acaacgttat tgacccaatt gtaaatgata ctaatgatgc
      781 aaaggctggt gaatgagtat gacttgctaa taaactgtta atggtcgttt gtataggtgg
      841 aaagaaacca aaggtaaaac ctagcaaaag ccaaaatatt aaatgctttt gattcgttaa
      901 taatatatta cacttgttga tttgattcat tgtgatgata ccaacaatta atagtagcac
      961 cccgattgct ttaattaaat taaaatcatg aattgtagca ccaaataagc caaatgtatc
     1021 aataatgaca cccataataa tttgacccgc aactgttgca atgacagtta atgttgcacc
     1081 taattttggc aataacaata aattgccagt taaatagcca acaccgagta atccgccaac
     1141 gaaccaagta tagttgaatg tttgattact aaaaaagtgt aatgagaaaa cgtcaggatt
     1201 tataaaaata tttaagacga ttaagcatat agtacctact gaaaatgaaa taaatgatgt
     1261 atagaaaggt gatttagtat aaagtgatag tcttgaattg acagaggttt gtatagggat
     1321 aaacattcca actatgaccc caatgatata gaaaagaagc atatgaataa agtctcactt
     1381 tccaaagtta ataataaaaa acatatgtat agattatcac tatagagaaa aaatacaact
     1441 catttttaag agagataaaa gtaaagcgag tcccataata ataccccagt caatcaagaa
     1501 gaaagactgg ggcgttaatg attatattca atgaatgact acctatgttt gagactattt
     1561 ctttgcttca aataaaacgg tcgtcgtttt atcaataatt tgagcagaat gtactttggt
     1621 gatagcgcca ctattagact ttaaaatatt taaatctact aatgcattca tactatcacg
     1681 tgcaacttgc tcggttacga tgttgtttaa ttgtggtaat tgcaatttga caggtttgtc
     1741 taatgaagat ttaaaactta attcaagtgt tttggtcatg aaagtgtcct cctgattaaa
     1801 ttgattcaga tttgatgact tcgatgttgt tgtatgtttc tccagtaagt cgctcgataa
     1861 gtttgctaaa tgttttaatt tggtcgtttg atacttcagg attaatattg gcgaatcgac
     1921 gtttaaattc tgtttgttta ccatttgtat ctagttttgt aaatgataat acaattgtaa
     1981 tgtgatttgt tttactcata ttttaaaacc tcctttcaca ctatatatcg aaagaaaaaa
     2041 ataattgggt taattttatt attcttgttt aaattctata aaaaaggtta taattatatg
     2101 tgacttaaat acttaatgtt tatgattaag acacttaaag caaggaggga catatgaaca
     2161 aggtagaagt aattaaatgc aatgatgata ttaataaaat gtatgacgcg ctcaaagctc
     2221 gatctgatag ggattatttg ttttttaaat tagctataca tagtggtatg aaaataacag
     2281 aattattaac aattactgtt gaagatacta aacgactgat tgaaaagggg acactaagcg
     2341 aattgtgtaa atcccattat cactcattga ttaaaattag attgccagaa acattatcta
     2401 atgagttact tcattatatt gaaaacaaaa gtctgtcaaa tgaagatgta ttatttcaat
     2461 cattgagaac taatcaagta ttatctaggc aacaagccta tcgtattatt catcaagcag
     2521 caattaaggc tggcatagag aatgtaggac taacgacatt gcgtaagaca tttgcgtatc
     2581 atgcatatca aaagggcata cctattcctg tcattcaaaa gtatttaggg catcaatcag
     2641 ctatagaaac attgaatttt atcggtttag aaaatgaatg tgaacacagt atttatattt
     2701 cattacaatt ataacgacaa aggaggctaa taatgagttt ggtttatcta ttaattgcca
     2761 tactagtaat tatggcgatg atattattta catctaaacg tagagcaatg gctaaatatg
     2821 caggatacat agcgctgact gcacctgtaa tagcatctat ttattttttg ttacaagttc
     2881 catcagtaat taaacagcat tatttgtctg tttccattcc gtggatgacc tcattagata
     2941 ttaatctaga tttacgttta gatggtttga gtttaatgtt ttcccttatt atttcactta
     3001 ttggaatcgc tgtattcttc tatgcaacac aatatttatc ctctcgtaaa gataatttac
     3061 caaggtttta cttatactta acgctattta tgtttagcat gctcggtatt gtattagctg
     3121 ataatacaat attaatgtat gtgttttggg aactaactag cgtatcatca tttctattaa
     3181 tttcatattg gtataacaac ggagatagtc aatttggtgc gatgcaatca tttatgatta
     3241 cagtatttgg tggtttggca ttattagttg gttttatcat gttgtatatc atgacaggaa
     3301 cgaataacat cacagagata ttaggacaag cagatcatat taagaaccat gctttattta
     3361 tccctatgat tatcatgttt ttattaggtg catttacaaa gtctgcacaa ttcccatttc
     3421 atatttggtt accaagggca atggcagcgc ctacacctgt aagtgcatat ttacattcag
     3481 ccactatggt taaagctggt atctttttat tacttagatt tacacctttg ttaggtctaa
     3541 gcaatatgta tatttatatc gtaacctttg tcgggttaat aactatgtta tttggctcaa
     3601 tcacagcttt aaaacaatgg gatttaaaag gaatcctagc atattcaaca atcagtcaat
     3661 tagggatgat tatggctatg gtgggtatag gtggcggata tgctcaacac caacaagacg
     3721 caatagcatc tatttatgta tttgtgttat ttgcagcatt gttccatctt atgaatcatg
     3781 ccattttcaa gtgtgcgctt ttcatgggtg tagggatttt agatcatgaa gcaggttcaa
     3841 gggacatacg aattttaagt ggcatgcgcc aattatttcc aaaaatgaat ctagtcatga
     3901 cgatagcggc tctatctatg gctggaatac catttttaaa tggattttta agtaaagaaa
     3961 tgttcctaga tgcattaaca caaactggac aattgtccca atttagtttg atttcaatga
     4021 ttatcatcgt atgtatgggt gttattgcta gtatttttac attcacatat gcactttaca
     4081 tggttaaaga agtattttgg actaaatatg attctaaagt gtttactaaa aaaaatatcc
     4141 acgaaccatg gttgtttagt ttaccctctc ttatattaat ggtgctagta cctgtaatct
     4201 tttttgtgcc aaatatattt gggaagggga ttatcgttcc agcattaaga ggtgtatcag
     4261 gtggtaatca tcaaattgat ccattggtac cacatgtttc gcaatggcat ggatttaaca
     4321 taccgcttct tttaaccatc atcattattt tattgggtag tgtactagca atcaaagtag
     4381 attggaaaaa agtgttcaca ggtaaaatta gacaaatttc agtttcaaaa gggtacgaaa
     4441 tggtttaccg acaatttgaa aagtttgcta cgaagcgatt taaacgtgtt atgcaagatc
     4501 gtttaaatca atacattatt atgacactgg gcatatttat ggtaatcatc ggatacggtt
     4561 atattcgtat aggactacca aaagtacatc agttacatgt ttccgaattt ggaccgttag
     4621 aagttatatt agcaattgtt acagttatca ttggtttgtc attaatattt atacgtcaac
     4681 gactaaccat ggtcatttta aatggggtca ttggttttgt tgtaacttta ttctttatag
     4741 caatgaaagc acctgattta gcattgacac aacttgtggt tgaaacgatt acgacaattt
     4801 tatttattgt tagcttttca agattaccca acgtaccaag gtctaaagtt aataaaaaac
     4861 gagaaataat taaaatttca gtatctctta tgatggcgct gattgttgtg tcacttattt
     4921 tcatcgcgca acaagcagat ggattggcat cgatttctaa tttttatctt agagcggata
     4981 aattaacagg tggcaaaaac attgtgaatg cgatattagg agactttaga gcattagata
     5041 cattgtttga aggtttagta ttaattatta ctggattagg tatttacaca ttattaaatt
     5101 accaagatag gaggggacaa gatgaaagag aatgatgtcg ttttaagaac ggtcacgaaa
     5161 ctcgttgtat ttattttatt gactttcgga ttctacgtct tcttcgctgg ccacaataac
     5221 ccaggtggtg gctttatagg tggtttaata tttagttccg cgtttatttt aatgtttcta
     5281 gcttttaatg tcgaagaagt attagaaagc ttaccgatag attttagaat tttaatgatt
     5341 attggtgcct tagtttcatc tattactgcg ataatgccaa cattttttgg aaaacctttt
     5401 ttgtcgcaat atgaaacaac attgacacta ccaattttag ggcacattca tgtaacgaca
     5461 ataacactgt ttgaattggg cattttgttc tcggttgtcg gggttattgt aacggtgatg
     5521 ctgtctctta gtggaggtcg atcatgaatt taattttatt actagttata ggatttttag
     5581 tatttatagg gacatatatg atattatcta taaatttaat tcgtattgtc ataggtattt
     5641 caatttatac acatgctggt aaccttatta tcatgagtat gggcacttac ggctctaata
     5701 aatcagaacc gctgattaca ggtggaaatc aattgtttgt tgatcccttg ttgcaagcta
     5761 ttgtactaac tgcaatagtt ataggttttg ggatgactgc gtttttactt gtcctcgttt
     5821 atagaactta taaagtaact aaagaagacg aaattgaagg cttaaggggg gaagatgatg
     5881 ctaagtaatt tgttaatatt accaatgcta ttaccattcc tttgtgcctt aatccttgta
     5941 tttttaaaaa ataatgaccg tatttctaaa tatttatact taggtacaat gactatcacc
     6001 acaattattt cattaatgct attaatttat gttcagcgtc atcgtccaat tacattggat
     6061 tttggtggtt ggactgcacc atttggaata cagtttttag gagattcgtt aagtttaatc
     6121 atggttacaa ccgcttcgtt tgtgattact ttaattatgg catatggatt tgggcgtggc
     6181 gaacataaag caaatcgtta tcatttacct tcatttatat tatttttaag tgttggtgtg
     6241 ataggctctt ttctaacatc agatttattt aatttatacg tcatgtttga aattatgtta
     6301 ctagcgtcat ttgtactcat tacacttgga caatctgtag aacaattacg tgctgcaatt
     6361 atttatgttg tcttgaatat tattggttca tggctattct tattaggtat aggtttactt
     6421 tataaaacag taggtacatt aaacttttca catattgcaa tgcgtttgaa tgacatggga
     6481 gataatcgca ctgttacaat gatttcatta atcttcttag tcgcatttag tgcgaaagca
     6541 gcgctggtcc tttttatgtg gctaccgaaa gcctacgctg tgttaaatac tgaacttgcg
     6601 gcattatttg cagcgttaat gaccaaagtg ggcgcttatg cattgattcg cttctttaca
     6661 ttattatttg accaacataa tgatcttata catccattgc tagcaactat ggctgctata
     6721 actatggtca tcggcgctat aggtgtcatt gcttataaag atattaaaaa gattgcagct
     6781 taccaagtca taatctcaat aggatttatc attttaggtt taggaacaaa cacgtttgca
     6841 ggtattaatg gtgcaatatt ttatttagta aatgatattg ttgtaaaaac attgctattc
     6901 tttattattg gtagtttagt ttacattact ggttatcgac aatatcaata tttaaatggt
     6961 ttagcaaaaa aagaaccatt ctttggtgtt gcatttatta taatgatttt tgctattggg
     7021 ggcgtaccac catttagtgg ttttccagga aaagtactta ttttccaagg tgcattacaa
     7081 aatggcaatt atataggact cacgttaatg attattacta gtttaattgc tatgtacagc
     7141 ttgtttagaa tacttttcta tatgtatttt ggagataaag atggcgaaga agttcatttt
     7201 aagagaatcc cacaatatcg taaaggtatt ttaagtattt ta
//
LOCUS       DAPIDM010000126         7167 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000126 DAPIDM010000000
VERSION     DAPIDM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.126"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1004)
                     /gene="adhP"
                     /locus_tag="RJE42_002183"
     CDS             complement(<1..1004)
                     /gene="adhP"
                     /locus_tag="RJE42_002183"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV5877583.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADVIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     "
     gene            complement(1503..2024)
                     /locus_tag="RJE42_002184"
     CDS             complement(1503..2024)
                     /locus_tag="RJE42_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDV5877584.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKVILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(2052..3347)
                     /locus_tag="RJE42_002185"
     CDS             complement(2052..3347)
                     /locus_tag="RJE42_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDV5877585.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGKIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(3387..3863)
                     /locus_tag="RJE42_002186"
     CDS             complement(3387..3863)
                     /locus_tag="RJE42_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877586.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(4033..4569)
                     /locus_tag="RJE42_002187"
     CDS             complement(4033..4569)
                     /locus_tag="RJE42_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDV5877587.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQGLDYQWIDVTQHRFKPIRDV
                     RHIEESITDYEDDYQEILDKVLASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTANKPGDI
                     MKDNYALARATEWNQLLQ"
     gene            complement(5015..5953)
                     /locus_tag="RJE42_002188"
     CDS             complement(5015..5953)
                     /locus_tag="RJE42_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV5877588.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINFLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVNALEKIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSKDEIQYIDELFPIED"
     gene            complement(6223..6849)
                     /locus_tag="RJE42_002189"
     CDS             complement(6223..6849)
                     /locus_tag="RJE42_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDV5877589.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKRYVKISKEDFENLNIVKPAKKNVFWPVAGISALFAVTLRKYTHLLDTQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNPKNERSKIILIPTLKNFCFTLFGYILFGGLT
                     MLFLDALLSMSHQNIIVYFTWIAVIMGFFLVNIALIIDKNIYVILKNQ"
     gene            complement(6873..7118)
                     /locus_tag="RJE42_002190"
     CDS             complement(6873..7118)
                     /locus_tag="RJE42_002190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877590.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDAGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
BASE COUNT         2285 a         1367 c          944 g         2571 t
ORIGIN      
        1 ttaataacca ttctaccagt tatagtacca ttttccattt cttcaaaaat atcattgatt
       61 tcttctaatt ttcttaattg aactttaggt gttactttat tttcagcagc aaattcaaac
      121 gcttcacgta agtcttgtct tgtaccaaca agtgaaccta ctacttcaat accgtcaagc
      181 actaatcttg ggatatctaa gttcatttta tcaacaggta aaccaacagc aacaactctt
      241 gcaccagctt ttacaacatc aaccgcttgg ttaaatggcg tcttagcaac tgaagttacc
      301 actgttgcat ctaatccttt gttatcagtt aatttcataa cttctgcaac tggatcaaca
      361 tctttagaat taataataac atctgcacct aattctttcg cgaatgctaa tttatcatca
      421 ttaatgtcaa aggcaacaat tttagccccc ataacgtttt tagcatattg taaagctagg
      481 ttacctaaac cacctatacc aaaaacacct aaccattgtc caggtttcac attacttact
      541 tttacagctt tataagttgt cacacctgcg catgtaatag aagacgctgc tgctggatct
      601 aatttttcag gtactttcac agcatagtct gcagtaacaa taacttgttc agccattgca
      661 ccatctactg tataaccagc atttttcaca ctacggcaaa gtgtttcacg acctgttgta
      721 caatattcac atcttccaca gctttcgaac atccaagcga tagacacacg gtctccaatt
      781 tttaatgatt ctacatcttc tgcaacttcg atgactttac caataccttc atgacctaaa
      841 gtaacgcctg taacatcacc aaaatcagca ttcttaacat gtaaatcggt atgacaaacg
      901 ccacaatatt ccgtttgtac caatgcttca ccaggtttta aagctcttaa ctttttgtcc
      961 tcaatactta ctttgtgatc tttcgttaca actgctgctc tcataataat gtcctcctta
     1021 tagtcactcc agtatgtatg atattaattg gcgattactt tcaaaaatga aaacgaataa
     1081 ttatagatgt aatttttatt tgtaatagac gccaaattaa aacacactac tgtgactttg
     1141 tttttagtta gatatcaacc gttaactttt aacgtttccc tagttaatat gttaaaagaa
     1201 atgacataat cgatatgcta acgtgtaatt tttacttcac atatatttat ttaaagataa
     1261 tttataatac tcttatcttc actcttatta taatgtgaat taattcacaa agtatatagc
     1321 tatgatcaaa aaaatgtgtc agttattcaa attttttatt aactacagat taatttaatt
     1381 tcaaaaaaat taaaaaaact cccaatcaat ctaagacttt attttcacct caagatttac
     1441 gtcgtcattg agaaatgaat catatcttta tagattaatt gggaaatttt agtttctaat
     1501 atttaattaa atgcttgatg taatgcatca gaagaatgct tccacatacc ttcatcatgg
     1561 ttcattaaaa agtcttttaa aataacttta ttctcgtcac caatttgatc aatgataata
     1621 tgatgtttca ttgatttatc catcatatta acatgttcag gcatactttt ataaccacga
     1681 cgaattgctt tattcactaa taaatagcac gctgttaaac cagcataata tggtccttgt
     1741 tcattacgtt ctgttgttac ccaacacaac caatattctt tagcttcatc gccttcaaca
     1801 gcagctttat cagtaatcca tttcacgcct ttttcaactt tggcgcgagc atgcatgcct
     1861 ccaatatcaa taaatgcttc tttgttagca acatcgataa aaactggtgc aatattgtct
     1921 aagctaattg aaccaatatt tgtaccttta tgaccatcta gagggtcatt ttttatgata
     1981 ttaaaattaa agcctttttt ctcagacaat gatataacct ccatatttaa cgataacaat
     2041 tttagccctg tttaattaag ctcattaatc aaattttcaa tagcttctct aattgtctta
     2101 tctttaatct ttgcaaccat ttctctagga taatataatt tatagtcttg gcgattaatg
     2161 cctgtaatac tatgaataac taaagattga ttgcttattt ctctaatctt accatcttgt
     2221 cttaataaat gaatcggttt gcgatttgac cccggtcgat catagtcata tggcaaatcc
     2281 gaaaatgctt cactcacaaa ataataatct ggattaatac cacctgcttc aaacagttct
     2341 tgcagttctg atattgtaat aattgagcca tcaaatggaa tatatttaaa taagtctcga
     2401 ttaataaatc gacttgctaa atcacttaaa atagcatcat cttcttttgt ccatttttcc
     2461 aaataatacg taactaccgc ttcatcgagt tcaacatatt gttcaatcgt aactgtctct
     2521 tcaaaaaatg gaataaaatc atgtggatgc aacttaaatt catagccttc attataaagc
     2581 tgttttgcgc gtttcaaaca attattaagc agcacttctc caccacgact aactgggtga
     2641 aaataaatct gccaatacat ttgataacga ctcataataa agttttcaac tgcatgcata
     2701 ccactttctt tgattagtac ttcatcttta gaaggtcgca ttaatcttaa aatacgctcc
     2761 atatcaaaag caccatatga tacacctgtg aaatacgcat cacgttgtaa ataatccatt
     2821 ctatccgcat cgatttgtga cgaaatcatc gaaatgacca atttattatg atgcgtttta
     2881 ttaattactt ccgcaacttc tcttggaaac tcaggcgaca ctttacgtaa tacagcattc
     2941 acctcagtat ctccagtaat aatcgcttgt gtgtatgctt catggtctgt attaaatatt
     3001 ttttcaaaac tatgtgaaaa tggaccatgt cctaaatcat gtaataatgc agcacataat
     3061 gccaacggtc tatctttatt gtcccatgca tcatgaccaa taaatgactc atcaattaat
     3121 cgtctaacta tttcatacac acctaaagaa tgtccaaagc gactatgttc tgctgtgtga
     3181 aaagataggt acagtgttcc taattgtcta attcgacgta acctttgaaa ttccttagtt
     3241 ttaattaaat cccatatcaa ttgatcttca acatgaatat atcgatgtat cgggtcttta
     3301 aaaacttttt cttctactaa ttttaaatct acatatgcgt tagtcattat tcccctcctt
     3361 ttcgtttaat ataatattta atttacttaa aatgctttgt acataagtgc taagtctaac
     3421 ttttcgccat acatttctgg ctcataagag cgtaagattg taaaaccttg ctctttatag
     3481 taagctactg cttcttcatt tttattatct acttctaagt aaacaccttc aaatttatct
     3541 tcaaaacgtg ataatccttc atttaacaat gctgtaccat aacctgtatg ttgcgattct
     3601 ggtttaacat aatgagctga taaatataat tcttcaccgt aaataaagtt agcaaagcca
     3661 acgatgtcat taccttcttc aacgactaag aataattgtt cttgaagtct tttctttaaa
     3721 tgatgttcat tatatgaagc ttctaacaag tgattaactg ttgtcgcagc gtatatattt
     3781 aaatatgtat taaaccaagc tttagttgcg acatctctaa tttgaacaac atctttttca
     3841 gttgcttgtc ttaccttgaa catgactttc tccccttatt aacaagtttt aataacggca
     3901 ttataccaca acttgctcaa tacttaataa acaatgattg tctattcaat ttatatattt
     3961 atattttccg ttaaaattaa aaataaaaaa taacgaagca aaaattcact tcgttttgta
     4021 atgaggtatt acttattgca atagttgatt ccattctgtc gcacgtgcta aggcatagtt
     4081 gtctttcatg atgtcaccag gtttatttgc tgtaccaata atatagccat ttaatgttgc
     4141 acctaaaaag tccaaactat atttcatttg cgttattgca ggtttcgctt taattttcgg
     4201 acaatctcca ccaactaaaa taactctaaa atctttttcg gccatttgtg ccttaaaatt
     4261 aggataacgt ttatcttgta gtgtttctga ccaatgttct ataaatgctt tcaatggtgc
     4321 tgaaatgcta taccaataca ctggtgatgc aaaaataatt gtatcacttg ccaatacctt
     4381 atctaaaatt tcctggtagt catcttcata gtctgtgata ctttcttcaa tatgtctgac
     4441 atcacgaatt ggtttaaacc gatgttgcgt tacatcaatc cattgataat ctaatccctg
     4501 caaagcaaat tttgttaatt gtgcagtatt accgtttggt ctacttccac caaacaaaac
     4561 agtaatcatt ttagcctaac ctcacttttg attgaaaatt atcgtttgtg atattgttac
     4621 ctaattatac tatcataagc cttgcctacc gaatagtaaa acgcttacaa cttttatgga
     4681 aatttgacaa aatttcgtca tgccttatat gacgttgttt gtggtgggag tgggacagaa
     4741 atgatatttt cgcaaaattt atttcgtcgt cccaccccga cttgcattgt ctgtagaaat
     4801 tgggaatcca atttctcttt gttggggccc atccccaact tgcattgcct gtagaatttc
     4861 ttttcgaaat tctctgtgtt gggcccctga ctagaattga aaaaagcttg ttacaagcgc
     4921 attttcgttc agtcaactac tgccaatata acttcgtaga gcatagaaca ttaatttatg
     4981 tcccagcctg atgtcaccat taaatacaat tcatttagtc ttcaattgga aacaattcat
     5041 cgatatattg aatttcatct ttagataaca caatatcagc agctttgata ttttcaatta
     5101 cttgttcggc acgttttgcg ccaggaataa ttacatcgat agctggtctc gttaaataaa
     5161 acgctaatac aatgttcgca attgaagttt gatgtgctgc agcaatcttt tccaaagcgt
     5221 ttacacgacg aacattttct tcaaatacac ctggtttaaa atcacgacgt gtagtacgat
     5281 ggtcactaaa tttagtgttc tcatcatatt ttccagctaa aataccggat gctaatggga
     5341 aatatggaat aaatgtgatt tggtgatcaa cacaatattg taatactgcc tcattttcgc
     5401 gatgcaataa attatattct aactgtacaa catcaacgta accatcttta tttgcttctt
     5461 taagttgatc taatgtgaaa tttgatacac caatggcttt aatcttccct tgttccttaa
     5521 gctcttgtaa tgctgcaact gcttgatctt tcggagtgtt gtcatccgga aaatgaatat
     5581 aatataaatc gatataatca gtttgtagac gtttcaaact attctcaact tgttgtttta
     5641 aatattccgg ttgattgttc tgatgtactt cttgattttc atcaaattca tgagaccctt
     5701 tcgtagcaat tttaatttgc tctcgcggat attctttaac aacttctcca accaattctt
     5761 ctgatcgttc tggcccataa atatatgccg tatctaaaaa attaatacca tgattaatgg
     5821 cttgacgaac aacatctttt ccttgttctt catctaagtt cggatataaa ttatgcccac
     5881 ctactgcgtt cgtcccaagt gcgattggaa acacttcaac atcagattta cctaagttta
     5941 caaattgctt cattagaccc agctccttat ctttataact tttccactat cacactacca
     6001 tgatttaata caacttccaa atgttagaat tagagcttga atttaagcgg tgtaatagga
     6061 aaacgttata taaaactcag ttaaaataag tattgcttaa attgatattc acatagcgcg
     6121 attgctcgtt agcctttaaa ttacatgtac ttttgaatca gaaattgaaa aacatttaag
     6181 aacgacaaca ttaaagccat tgcaaattca atttggtaat ttctattggt ttttaagtat
     6241 gacataaatg tttttatcta tgattaaagc tatattcact agaaaaaaac ccattataac
     6301 tgcaatccaa gtaaaatata ctattatgtt ttggtgactc attgataata gtgcatctag
     6361 gaatagcata gtcaatccgc caaataaaat atatccaaat aatgtgaaac aaaaattttt
     6421 tagtgttggt attaagatga ttttcgacct ttcattttta ggattataaa tatttaaaga
     6481 tgatttttta attaggcgta cataaaatat taaaatccct ataaatgtga tgcaacatat
     6541 ggcaataact aattttttat caagttgagt gtcaagtaaa tgcgtatact ttcttaatgt
     6601 aactgcgaat aaagcagaga tacctgcaac tggccaaaaa acattctttt tagctggttt
     6661 aacaatattt aagttttcaa aatcttcttt actaattttg acgtatcttt ttgggatgaa
     6721 ccaattgata aatggaaaaa ataaactaat ccaagtgctt attatatcga ccattaagta
     6781 ctcatcatta tatttaataa ttctatattt tggattttta ttgatgattt tagattcgca
     6841 tagcaacatt taccagctcc atctcttagg gcttatatga ttagaaatac tttttgaagc
     6901 ttcaccatga cattttcaac accatctact gttttttctg cagatttttc tacagcatct
     6961 ataccagcgt ctagtttatg tcctatccaa tcgtttttat cttttattcc aaattcattt
     7021 ttttgattgg tccaatatac aagtattgcc gaaatagaac ctgcagtaaa tccagctaaa
     7081 ccatcatttt tccaccgtgt tagttttaga taatacataa atttttttgt caataatcga
     7141 tgacatgttt aacagaaaga aacctgc
//
LOCUS       DAPIDM010000127         7159 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000127 DAPIDM010000000
VERSION     DAPIDM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.127"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(16..1344)
                     /gene="cstB"
                     /locus_tag="RJE42_002191"
     CDS             complement(16..1344)
                     /gene="cstB"
                     /locus_tag="RJE42_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDV5877591.1"
                     /translation="MFFKQFYDKHLSQASYLIGCQKTGEAMIIDPIRDLSSYIRVADE
                     EGLTITHAAETHIHADFASGIRDVAIKLNANIYVSGESDDTLGYKNMPNHTYFVQHND
                     DIYVGNIKLKVLHTPGHTPESISFLLTDEGAGAQVPMGLFSGDFIFVGDIGRPDLLEK
                     AVKVEGSSEIGAKQMFKSIESIKDLPDYIQIWPGHGAGSPCGKSLGAIPTSTLGYEKQ
                     TNWAFSENNEATFIDKLISDQPAPPHHFAQMKKINQFGMNLYQPYTVYPATNTNRLTF
                     DLRSKEAYHGGHIEGTINIPYDKNFINQIGWYLNYDQEINLIGDYHLVSKATHTLQLI
                     GYDDVAGYQLPQSKVQTRSIHSEDITGNESHILDVRNDNEWNNGHLSQAVHVPHGKLL
                     DTDLPFSKNDVIYVHCQSGIRSSIAIGILEHKGYHNIINVNEGYKDIHLS"
     gene            complement(1363..2430)
                     /locus_tag="RJE42_002192"
     CDS             complement(1363..2430)
                     /locus_tag="RJE42_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HDV5877592.1"
                     /translation="MSQYKTKHINDFTKKELEELGSKDQLIDVRQPEEFELGHIKNAL
                     LHSVENIETFKQSKNKTYYIYCKSGNRSRKASQFLAQRGYDVVNLDGGYTAYEEQHHN
                     DNLSKVKEDREIKDNRKTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLND
                     IKSWVKQTGHTLVRLNDFGNEIHAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAA
                     MIIANGAKAAGRDVTIFCTFWGLNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISK
                     MNMFGLGNLMMRYVMKKKNVDTLPSLIDQAVEQGVKLIACTMSMDVMGITKEELRDDV
                     EYGGVGAYIGYTEQANHNLFI"
     gene            2569..2829
                     /gene="cstR"
                     /locus_tag="RJE42_002193"
     CDS             2569..2829
                     /gene="cstR"
                     /locus_tag="RJE42_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDV5877593.1"
                     /translation="MEYNKKMINRIHRIQGQLNGVIKMMEEEKNCKNVISQLSASKSS
                     IQRLMGIIISENLVECVKMSEENSEDSQALINEAVELLIKSK"
     gene            2826..3584
                     /locus_tag="RJE42_002194"
     CDS             2826..3584
                     /locus_tag="RJE42_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV5877594.1"
                     /translation="MISITTIIVMLVIGILGGFISGLVGIGGAIVIYPALLLLPPLFG
                     LPTYSAYIASGLTSSQVFFSTLSGSLNAYKNKNFSRTLILNMGSGMVIGSMLGAILAN
                     LINVQFVNTVYIIIALLALILMFIKVTPSTSHIKFNRLLLITIGGIIGLVSGIVGAGG
                     AFIIIPVLLVIFKLPMNMVVTNSIVIAFISSIGAFMIKLLQGYIPINSAIPLILGSIL
                     FTPLGMKMGQKIPDYIQKTIICILIVIAIIKLIF"
     gene            3754..3963
                     /locus_tag="RJE42_002195"
                     /pseudo
     CDS             3754..3963
                     /locus_tag="RJE42_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            3981..4526
                     /locus_tag="RJE42_002196"
     CDS             3981..4526
                     /locus_tag="RJE42_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HDV5877595.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMTSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VSNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            4807..6597
                     /locus_tag="RJE42_002197"
     CDS             4807..6597
                     /locus_tag="RJE42_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HDV5877596.1"
                     /translation="MEKFKEKVNDLEAIKTTRYYTEFVEKFKKIRDWAKNENLKNEAK
                     LAQYEIEIFSLCEKNPILSKNKSEKRFVATISFDDGREWPDIQKFTNDQIKYYEQRLN
                     ETNNLFLKVRYSDFLFEHGNKKITKTKYEISKCLLSCLVEILTYYSDDFNYTSVLARL
                     VEVSLLMGDREKLEKAIELIYLKMDEFDYNNEYSYVYELSKLIREILKSKHKKIILEN
                     HLNKIIIVLEKAIKNNFEDKNYYLHRVFCEELSQYRKFDLISSERRSELKKEIGKSYE
                     LEAEYQQGRNNKSLLVKANFLEKAMEKYMEIGEREKSNKMKILVKWTYEEYENSNEMN
                     LIRIPIEFPKEEIDKIIEGFISSDVQISLDKIAYSNDLIPKITVIEDLVDKLSKEFPL
                     QGLISKGLLNDGKKVVETTTEEDNKTINFNSNYMHHLNINVELLLKAIFDKLISEFEI
                     STEDFMQKFNKWELLDNKNVPFIEHGISRFLEKDYLSALHILVPQFESTVRRMFSKAG
                     YSTTSIRKGNTQQEVTFNEFLLRDDVKSTLGNDVHKLIQIVMVEQSGLNLRNEIAHGL
                     IELSNINYTKCILVIYLFLILTRYSIEKND"
     gene            6638..>7159
                     /locus_tag="RJE42_002198"
     CDS             6638..>7159
                     /locus_tag="RJE42_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877597.1"
                     /translation="MKLEELLLITQQPLIEYSKNKKLLEGSRLFDIDERMDERKIPKI
                     LTNSDKYHVVEIANYDNLIIIDNEVINGNELIQVGQCIDFDSNVMSYLRNLIVNNKYE
                     DDFFKILTNIKKSKQQISCVPYLIENGNNIHRINKIISYETILSFSIFDRISEFDFEN
                     RNFSRYFNDSEVIL"
BASE COUNT         2639 a          911 c         1155 g         2454 t
ORIGIN      
        1 gtataaatat aattattaag aaagatgtat atctttgtaa ccttcgttta cattaataat
       61 gttgtgataa cctttgtgtt ctaaaatacc aatagctata gaacttctaa tgccagattg
      121 acagtgtaca taaataacat catttttact gaaaggtaaa tctgtgtcta aaagtttgcc
      181 gtgtggtaca tgaaccgctt gagataagtg gccattattc cattcattat cattacgtac
      241 atccaatata tgtgattcgt tacctgtaat atcttcacta tgaatggaac gtgtttgaac
      301 tttagattga ggtaattgat atccagcaac atcatcgtat ccaatgagtt gcaaggtgtg
      361 cgttgctttt gaaacaaggt gatagtctcc aatcaagtta atttcttgat catagtttag
      421 ataccagcca atttgattga tgaaattttt atcatatgga atattgattg taccttcaat
      481 atgtccacca tgatatgcct ccttactgcg gagatcaaaa gttaatctgt ttgtatttgt
      541 agctggataa accgtataag gttgatataa attcataccg aattgattaa tttttttcat
      601 ttgtgcaaaa tgatgtggtg gtgcaggttg gtcagaaatg agtttatcga taaaggtagc
      661 ttcgttattt tcagaaaaag cccagttcgt ttgtttttca tagccaagcg tagatgttgg
      721 aatagcacct aaagatttac cacaaggact accagcgcca tggccaggcc aaatttgaat
      781 gtaatctggc aagtctttaa tactttcaat cgatttaaac atttgtttag cgcctatttc
      841 agatgatcct tctactttaa ccgctttttc tagtaaatca ggtctaccga tatctcctac
      901 aaaaataaaa tcaccactga atagtcccat tggaacttgt gctccagcac cttcgtcagt
      961 aagtaaaaaa cttatacttt ctggcgtgtg acccggtgta tgaagcactt ttaattttat
     1021 atttcctaca taaatatcgt cattatgttg aacaaaataa gtgtggttag gcatattttt
     1081 ataacctaat gtgtcatcac tttcacccga tacataaata ttagcattta actttatagc
     1141 aacatctcta attcctgaag caaaatctgc atgtatatgt gtttcagctg catgagtaat
     1201 ggttaaacct tcttcatcag caactcgaat atatgaagat aagtcacgaa taggatcaat
     1261 aatcatggct tctccagttt tttgacaacc gattaaataa gatgcttgag ataaatgttt
     1321 atcataaaat tgtttaaaaa acataatatc attcctttct aattatataa ataaattgtg
     1381 attagcttgt tctgtatatc caatatatgc acctacgcca ccgtattcta catcatcgcg
     1441 tagttcttct tttgtaatgc ccatcacatc catactcatt gtacaagcga taagtttaac
     1501 accttgctca actgcttgat caataagaga aggtaaagta tcgacatttt tctttttcat
     1561 gacataacgc atcattagat tgcctaaacc aaacatattc attttagaaa taggcatgtt
     1621 aattggtgaa tttggaagca tgaagtcaaa aagttttgca ataccttttt tcttaatgcg
     1681 ttgactttga atttttttaa gtgcatttaa gccccaaaat gtgcaaaaga tggttacatc
     1741 tctacctgca gctttagcac cattagcaat aatcattgct gcaactgctt tatctagctc
     1801 gccgctaaat aaaacaattg ttgttccact tttcgtgtgt gtcacttcta tatttttatt
     1861 ttcttctttt tgaataatag cgtgaatttc attaccaaaa tcgttgagtc taacaagcgt
     1921 atgtccagtt tgtttgaccc aacttttaat atcatttaaa aatccgtgat cagtcacaac
     1981 aacttctatt tgatcaccaa tagcgatgtt tttgatttct ttactaatat taacaatagg
     2041 tcctggacac tgtagattac tataattgaa tgtcttacga ttatctttaa tttctctatc
     2101 ttcttttact ttagataaat tatcattatg atgttgttct tcgtatgcgg tataacctcc
     2161 gtctaaattc accacatcgt atccgcgttg tgcaagaaat tgactggctt ttctacttct
     2221 attaccactt ttacaataga tatagtaagt tttatttttg ctttgtttaa aagtctctat
     2281 attttcaact gaatgtaata aagcattttt aatgtgtcct aattcaaatt cttcaggctg
     2341 tcgaacgtca attaattgat ccttagaacc caattcttct aattcctttt tagtgaaatc
     2401 attaatgtgt ttcgttttat attgtgacat aattacctcc ttttaaatac ctatgggggt
     2461 atattataac gaattagata gtttgtcaaa tacctatggg ggtatttgac aaaataaaat
     2521 ttaatcaata tgatgtaatt attaaaaatg aatgtggagt gaaatatatt ggaatataat
     2581 aaaaagatga ttaatcgtat tcatcgcata caaggacagc ttaatggagt cattaaaatg
     2641 atggaggaag aaaaaaattg taaaaacgtc attagtcaat taagtgcttc taaaagttct
     2701 attcaacggt taatgggtat tatcattagt gaaaacttag tagaatgcgt caaaatgtct
     2761 gaagaaaata gtgaagattc tcaggcacta attaatgaag cagttgaatt attgataaaa
     2821 agtaaatgat aagtataacc accattatag taatgttagt tattggtatc ttaggaggat
     2881 ttatttctgg attagtcggt atagggggag caattgttat ttacccggct ctcttattat
     2941 taccaccatt atttggttta cctacttata gtgcttatat tgcttcagga ttaacttcaa
     3001 gtcaggtgtt ttttagtaca ttgagtggat ctttaaacgc atataaaaat aaaaattttt
     3061 ctagaacgct tatacttaat atgggaagtg gaatggtaat aggtagtatg ctaggtgcaa
     3121 tcttagcgaa tttaataaat gttcagtttg ttaatacggt ttatattatt attgcattac
     3181 tagctcttat tctaatgttt ataaaagtta caccatctac tagtcatatt aagtttaatc
     3241 gtctactttt aattacaata ggtggtatca tcggtttggt atctggtatt gtaggagctg
     3301 gtggcgcatt tattattatt ccagtactac ttgttatatt taaattacca atgaacatgg
     3361 ttgttacaaa tagtattgta attgcattca tttcttctat tggagcattt atgattaaat
     3421 tattacaggg ttatattcct attaatagtg caataccgtt aattttaggt agtatactat
     3481 ttacgcctct aggtatgaaa atgggtcaga aaatacctga ctatattcaa aaaacgataa
     3541 tatgtatttt aatcgtcatt gcaattatta agttgatatt ttaaaaacat gggggtggga
     3601 caacattcat tttgaacttg attgaatata tagtatattt aataaaatgc agctattgtg
     3661 gcgtagaatt taagaattaa aaaatgatta atatagtttg aaaagactga atataaatac
     3721 tagaaaaata gccagtaaat gagtttatta tgaattcatt tcttgactct ttcaaataat
     3781 actatgagaa aaatggtgca aaacttatgg tggggtgcag gttataatat tgttgctgta
     3841 cctttagcag ctggtatttt agcatttatc ggcttgattt tatcacctgc aataggtgct
     3901 attttaatgt cactaagtac ggttatcgtt gcaattaatg cctttacatt aaaattaaaa
     3961 taaaagatag gagttttatt atgattaaaa aattattttt tatgatatta ggatcattac
     4021 taatattatc agcttgctcc aataatgatg aaaaagataa agacactaat gaccaaaaaa
     4081 gtgagagcca tatgaagcat aatgatgaaa gtaaagttcc tgaagatatg acatcgacta
     4141 atgagggtga atttaaagta ggagataaag taacgattac agcagggcat atgccaggta
     4201 tgaaaggtgc agaagctact gtaaaaggtg cgtataaaac atatgcctat gttgtaagtt
     4261 ataaacccac aaatggaaat gaaaaagtaa gcaatcataa atgggtcgta aacgaagaga
     4321 tcaaagatgc acctaaagat ggatttagta agggcgatac tgttaaatta gaagcaagtc
     4381 atatgtctgg tatgaaaggt gctacagcca atatagataa cgtgaaaaag acgactgttt
     4441 acgtagttga ttacaaatcc aaagataatg gtaaaatcat taaaaatcat aaatggatga
     4501 caggaaatga attgaaagca cgataaaaat ctagttctag attgagaaat aaatagatat
     4561 aaagatatcc tccttaatca ataatttaaa taacttatta ttgttaagga ggatattttt
     4621 tagtgtgtaa attaaaaaga attttagaag aataacattt atcaaaaaac tgttcattac
     4681 cttattaaat gaaattatat aattaaaaat cgcatcatta accgatacgc agaagcatat
     4741 cataaataaa actaaaaatt aaatataacg attaatattt taatttcaat gaggtaagat
     4801 taaggtatgg aaaaatttaa agaaaaagta aatgacttag aagcaattaa gactactaga
     4861 tattatacag aatttgtgga gaaatttaaa aaaattcgag attgggctaa aaatgaaaat
     4921 ctaaagaatg aagcgaaatt ggcacaatat gagattgaaa ttttctcttt atgtgagaaa
     4981 aatcccattt tatcaaaaaa taaaagtgaa aaaagatttg tagcgacaat tagttttgat
     5041 gatggtagag agtggccaga cattcaaaaa tttacaaatg atcaaattaa atactatgag
     5101 caaagattaa acgagacaaa taatttgttt ttaaaagtaa gatattctga tttcttattt
     5161 gaacatggta ataagaaaat aactaaaact aaatatgaaa tttcaaaatg tttgctatcg
     5221 tgcttagttg agattcttac atattacagt gacgatttta attatacttc agtattagct
     5281 agattagttg aagtatctct tttaatggga gatagggaaa aactcgaaaa agctatcgaa
     5341 ttaatttatc taaaaatgga tgaatttgac tataataatg agtatagtta tgtatatgaa
     5401 ctttcaaaac ttataagaga aattttaaaa tctaagcaca aaaaaataat cttagagaat
     5461 catttaaata aaattataat tgttttagaa aaagctatta aaaacaattt tgaagataaa
     5521 aattattacc tacatagagt attttgtgaa gaacttagtc aatacagaaa attcgattta
     5581 atttcttctg aaagaagaag tgaattgaaa aaagaaattg gaaaaagcta cgagttagag
     5641 gcggaatatc aacaaggaag aaataataag agtttattgg taaaagcaaa ttttttagaa
     5701 aaagctatgg aaaaatatat ggaaattgga gaaagagaaa aaagtaataa gatgaagatt
     5761 ttagtaaaat ggacctatga agaatacgaa aacagtaatg aaatgaatct aataagaatt
     5821 ccaatagaat ttccaaaaga ggaaattgat aaaattattg aaggatttat ttcctcagat
     5881 gtacagattt cgttagataa aatagcatac agtaatgact taattcctaa aatcacagta
     5941 attgaagatc tagttgataa gttaagtaaa gagtttcctt tacaaggttt aatttcaaaa
     6001 ggtttattga atgatggaaa aaaagtagtg gaaactacta cagaagaaga taataaaaca
     6061 atcaatttta atagtaatta tatgcatcat ttaaatatta atgttgaatt acttcttaaa
     6121 gctatttttg ataaattaat atcagaattt gaaatttcca ctgaagattt catgcaaaaa
     6181 tttaataagt gggaactatt agataataaa aatgttcctt ttattgaaca tggaatttct
     6241 cgatttttag aaaaggatta tttgagcgct ttgcatattt tagtccctca atttgaatct
     6301 acagttagaa gaatgttttc taaagctggc tattcaacta catctattag gaaaggaaat
     6361 acgcaacaag aagtaacatt taatgaattt ttactcagag atgatgtgaa atcgacctta
     6421 ggaaatgatg tgcataaact aattcaaatt gtgatggttg aacagtcggg gttgaattta
     6481 cgcaatgaaa tagctcatgg attaatagaa ttatcaaata taaattatac gaaatgtatt
     6541 ttggttattt atttattttt gatattaact agatatagta ttgaaaaaaa tgactaagtg
     6601 ataatttata ttatacaagt tttgtggagg acatatcatg aaattagaag agttgctact
     6661 aatcacacaa caaccattaa ttgaatattc taaaaataaa aaattattag aaggatcacg
     6721 tttatttgat attgatgaaa gaatggatga aagaaaaatc cccaaaattt taactaatag
     6781 cgataagtac catgttgttg aaatagccaa ctacgataat ttaataatta ttgataatga
     6841 agtaataaac ggtaatgaat taattcaagt cggtcaatgt attgactttg atagtaacgt
     6901 aatgagctat ttacgaaact tgattgtaaa taataaatat gaggatgatt tttttaaaat
     6961 tttaactaat ataaaaaaaa gtaagcaaca aataagctgc gtaccttatt taatagaaaa
     7021 tgggaataat attcatagga taaataaaat aatttcatat gaaacaatat tatcattttc
     7081 tatatttgat agaataagcg aatttgattt tgaaaatcgc aatttctcaa gatattttaa
     7141 tgattcagaa gttatttta
//
LOCUS       DAPIDM010000128         7147 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000128 DAPIDM010000000
VERSION     DAPIDM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.128"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(73..336)
                     /locus_tag="RJE42_002199"
     CDS             complement(73..336)
                     /locus_tag="RJE42_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5877598.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            630..881
                     /locus_tag="RJE42_002200"
     CDS             630..881
                     /locus_tag="RJE42_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV5877599.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWLG
                     GTLLGHWGPELGSIYILPALIGSIILIALVTLILRAMRK"
     gene            complement(923..1131)
                     /locus_tag="RJE42_002201"
                     /pseudo
     CDS             complement(923..1131)
                     /locus_tag="RJE42_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1309..1890
                     /locus_tag="RJE42_002202"
     CDS             1309..1890
                     /locus_tag="RJE42_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV5877600.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTETGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNNVSREEAVDLKVENCKPYIIE"
     gene            complement(1946..2329)
                     /locus_tag="RJE42_002203"
     CDS             complement(1946..2329)
                     /locus_tag="RJE42_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877601.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKAFDKNDSH"
     gene            complement(2599..3225)
                     /locus_tag="RJE42_002204"
     CDS             complement(2599..3225)
                     /locus_tag="RJE42_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDV5877602.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(3297..4919)
                     /locus_tag="RJE42_002205"
     CDS             complement(3297..4919)
                     /locus_tag="RJE42_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877603.1"
                     /translation="MLDVHFIYPEKLQNACEYFNLPTQKEGFEVTIHALRPENLHKIY
                     HDMDKTEQKFLETLISNGNPVTIINSHEVNILKLTNHLGFVIQINEDTFFVNNLFLSY
                     INSEVLDSAYGEFETLDTKDHMHLNDEIFRVRQHTRLNDVTMEQALLLKTNDELKAIC
                     RQYGIKGFSNKNKQNLVKLIMTAFFNNNHIIEQVFSNASLFEFSILTGILESDDNYRL
                     NNELITFENIHLMNPGTFLITHFYYYYDFEYDCLILPNDVRKHIENYVNQIGDGDIKK
                     AINKFIDEQTIKHSLADFENAIELSMMDDEAHFDILYNEDDDFEDFGTTDSLRSDAIT
                     QLISEIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSL
                     DTLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLK
                     SDQAIKEFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLVQPIMQLFIDNAILN
                     NVQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            complement(5034..6557)
                     /gene="ahpF"
                     /locus_tag="RJE42_002206"
     CDS             complement(5034..6557)
                     /gene="ahpF"
                     /locus_tag="RJE42_002206"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV5877604.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAILESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(6573..7142)
                     /gene="ahpC"
                     /locus_tag="RJE42_002207"
     CDS             complement(6573..7142)
                     /gene="ahpC"
                     /locus_tag="RJE42_002207"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDV5877605.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
BASE COUNT         2334 a         1105 c         1208 g         2500 t
ORIGIN      
        1 aacgcaatgt aatatatcca gtatagattt gaaaggatat atgtctaacg tgttgtgagg
       61 gtttaattgc atctatttat ccatgcgaat atcgacttct tctaaatgtt tctgatattc
      121 tttaacctta ctttctaaaa acatttcata tggtgcatca aagaagtcag ctaaatgcat
      181 ggcatcttta aatttaggtt catggtgatg attctcccat tcccaaattt gatgtgcttc
      241 atatttagta ccatatttct catttaattg ttgtgctaat tcgtcaattt ctaaattatg
      301 tttagttcgt aagttatata aaatatgcat attcattgtt cattaacctc gcttcttgat
      361 ttaaaattca aagatgctct aataatatgt gtatattcta gtcctataac ataaaataat
      421 caagctatga attcggcaga aaaaataatg tatatgatat tgctggtaca gagactttaa
      481 ataacgatag ctacattgaa taaaattgat attcaattac tactttgaaa aatatttaga
      541 taaaaataat ttgaatttgt tttagaaagt ctaataatgg gtactactta gaataacgca
      601 aaataaatag aaaaaggaga ctgaaaatta tgtttggatt tattggaatg ttaattgtcg
      661 gtggcttaat tggatgggct gctggtgcta ttatgggtaa agatatccca ggtggtattt
      721 taggtaatat tatcgcaggt attattggat catggttagg tggaacatta ttaggacatt
      781 ggggtcctga actaggaagc atttacatct taccagcttt aattggttca attatcttaa
      841 ttgcactggt aactttaatt ttaagagcta tgcgtaaata ataaaaaatt caacttaggt
      901 tgaattgaaa taaagatggc attcagttgt catctttttt atttgcgtga aaatggtcaa
      961 tatatattat aatatttgaa aatgtaggta agatagaatt gaaaagtaat aaattaatat
     1021 gaggaaattt tggaaataaa aaagcgagta tttgacaaat aaaaaaggat gacgttgggt
     1081 tgcgtcatcc aggagtatta tgttcatgaa tataaaagac tgtgtagtca tttaagaact
     1141 acacccatat agtaacacat cacttgatta aagacaatac tatttttaga gtcattaatt
     1201 taaaatatga attaattatt tttttagaat agaatattaa gattgatatc gaattagtag
     1261 tcaaagtgtt atggtagata tgaaatacat aaggtgagga gtaatattat gacgatttat
     1321 ttagttagac atggcgaatc aaaatcgaat tatgataata aacattttcg atcttatttt
     1381 tgtggacaat tagatgtgcc gttaacggaa actggcacaa aaagtgcaga cgatttatgt
     1441 gattatttta aagagaaaca gattaaacat gtatatgttt cagacttatt aagaacacag
     1501 caaacgtttg agcatatttt tccatatgac attgcatcaa cgactacgcc tctattaaga
     1561 gaacgttcac ttggcgtatt tgagggtgaa tataaagatg aaatcagtgc gaatccgaaa
     1621 tatgaaaaat atttcaatga tccaaacttt aaagactttc gtcatagttt ttcacaaaaa
     1681 gcgcctgaag gagaaagtta tgaagatgta tatcaacgcg tagaacattt tatgaatcat
     1741 gttgtcaatg aagatacaca aaaagatgat attgttattg ttgcacatca agttgtcatt
     1801 cgttgtttaa tggtttattt taataacgtt tcacgggaag aagctgtgga tttaaaagtt
     1861 gaaaattgca aaccatatat cattgaatag aagtatagag gttctgaaaa gctgtgtttt
     1921 atagcggtct tcagaacctt ttttgctaat gggaatcatt cttatcaaaa gccttaccac
     1981 caatagctaa tgtgataatt gcgcttatga acaaaataat tgaagttata aatgacgcta
     2041 atgtaaatgg tgtaaattgt gtgagcgata atatcaatga taaaaagaac caagagatag
     2101 cccctagaaa ttgtaaaata gcagaagaaa ttctaaaact catataatca tatatagcta
     2161 aaacaagtga aggtaatgcg actaatacca ttataatgat gttgagggtg aataaatatg
     2221 gctgttcaaa agttactgtg tttggtcctg ttggatgcat tgctgctaag aacaagcaaa
     2281 caatcgtcga taaaattgct aaaataataa aagtgattcg aactttcatc atgaccatcc
     2341 tttgtttata gagtcaatat aagtatggaa tatgttaggt atatagtcaa atgcgttaac
     2401 taatgggtga ttttggcata gatagagaat ttaagaccaa taaaaaagca tcaaacagta
     2461 ataagctgct tgatgcaaaa tggtatggta ttggttaaaa tgtattgttt caaagattag
     2521 tcacttttaa agataaagaa ttgtcatgaa ttaaagcaca tgtaatgatg tgttacatgt
     2581 cgcaatgatg gctttcagtt atttatcgat aacatcactc ttgatacctt tagattttaa
     2641 gaaatcttta attttatctt gttgcttttt attaacatca ccggcatatt ttgttggcac
     2701 gtcgacaaca ttgattttat tttgcggttg atagctaagc ttttcaatat cttcatcaac
     2761 attggcgatt gtactattta aagctttgaa gtaattcatc attaattcaa cgggtttctt
     2821 atattcttta ggaatattgt tttcagtgac aaatttcttg aaatgcaaat cgtttttaac
     2881 agctaagtta gataagtggc taagtgtttc tgcttgtttt tcagtcactt ttgtttgact
     2941 gtcaatttgt ttatctagtt tatgttgcat aatatatttg ttatcaagta tatcgctatt
     3001 tacagacaaa tacttttcta tagcttgctt catctctgca tcactaatat cactattttt
     3061 cttatctgag ttaaagatat cttttgtttc taatttttta gcgcttttag gtgcatggat
     3121 gccagtactt gtgtgatgat cttcgttatc agattgatcg gacgcgcaac ctgtaagaat
     3181 taatgtcgat gctaaaaatg tacttagtag taatctcttt ttcataatgt aatataactc
     3241 cttagtttat ctttaattga aaaaatatgt attcatgcta atagagtaac gttgaattag
     3301 tttggaatgt cacgatgacc ttgcaatgac catagacgta aatgattacg tgcatgagtt
     3361 gctttttcta tcaataatgc gtcgttttga acgttgttaa ggatagcatt atctataaat
     3421 aactgcataa ttggttgtac taatttagac gtaggtatcg tacgtaaaag cataatgatt
     3481 tcattcacat acttttcctt ctcaatatca tttttcatat tgatttgttt gcgtagataa
     3541 ctggtaaact ctttaattgc ttgatcagat ttcaaatagt ggtgtctatc ataatagatt
     3601 aattcctcaa cagaagctgg tacatagtat ccttcatcag aaatatcaat gcctgacgcc
     3661 tcagactcga caacatcaaa taacacaggg tgaaacacga agtcatcttc aatataatat
     3721 agctcagtac cagctaaagt atctaagcta tattgcaact cttctttact aaaagtagcg
     3781 tcataaaatc tttgtgtgag atatagtaga tgattagttg ataaaatacc atacaagttt
     3841 acagaaccaa gtataatacg aagaggtaaa atatcttttt tatctattaa tcctttctta
     3901 atctcagaaa tcaattgagt gattgcatcg cttcttaagc tatcggttgt accaaaatct
     3961 tcaaagtcat catcttcatt atatagtata tcgaagtgag cttcgtcatc catcattgat
     4021 aattctatcg cattttcaaa atctgctaaa ctatgcttaa tcgtttgctc atcaataaat
     4081 ttattgatag ccttctttat atcgccgtca ccgatttgat tcacgtaatt ttcaatatgt
     4141 ttacgcacgt catttggtaa aatgagacaa tcatactcga agtcataata ataatagaaa
     4201 tgagtaatta aaaatgtgcc aggattcatc aaatgaatgt tttcaaatgt aattagttca
     4261 ttgtttaatc gatagttatc atcactttcg agtatgccag taagaatact aaactcaaat
     4321 aaggaagcat tagaaaacac ttgttcaatg atatgattat tattgaaaaa tgctgtcata
     4381 atcaatttaa caagattttg cttgttttta tttgagaatc ctttgatacc atactgtctg
     4441 caaattgctt ttagctcatc attagttttt aacagtaaag cctgttccat tgtgacatcg
     4501 tttaagcgcg tatgttgtct tacacgaaag atttcatcat ttaaatgcat atggtctttt
     4561 gtatctaatg tttcgaattc accatatgca ctatctagta cctctgaatt gatataactt
     4621 aaaaataaat tatttacaaa gaacgtatct tcgttaattt gaataacaaa acctagatga
     4681 tttgttagct ttaaaatatt aacctcatgg ctatttatga ttgtgacagg attaccatta
     4741 gatataagtg tttctaaaaa tttttgttct gtcttatcca tatcatgata aatcttatgt
     4801 aaattttcag gacgtaaagc gtgaatcgtg acttcaaatc cttctttttg ggtcggtaaa
     4861 ttaaaatatt cgcatgcatt ttgtaatttt tctgggtata taaaatgaac atcaagcatt
     4921 tttgcacacc tcgttgtata tattaagttt atcataacat gattttatgt cgggataaaa
     4981 aaataacagc atcttaacaa atgtaagata ctgtcagtga aatgaatgaa actttagttt
     5041 ctgataatat agtcaaaggc atttaatgct gcatttgcac cagcgcccat tgaaataata
     5101 atttgtttgt tcttctgatc tgtgacatcg ccagcagcaa atattccagg tacgttagta
     5161 ttattgttac gatcaataac aatttcacca cgttcgttta attcaacagc atcttttaac
     5221 catgatgtgt ttggaagtaa accaatttga acaaagatac catctaagtt aagtagatgt
     5281 tcttcgccgg tgctcatgtc ttcgtaacgt atacctgtaa catggtcttc tccgacaact
     5341 tcagtagttt tggcatttgt tttgatatca acatttgata aagaacgtaa acgatcttgt
     5401 aacacgttgt ctgcttttaa ttcgctagcg aattcgaata atgtaacatg attaacgata
     5461 ccagcaaggt caattgctgc ttcaacccca gagttaccgc caccgataac tgctacgtct
     5521 ttattttcaa atagaggtcc gtcacagtgc gggcagaatg caacaccttt attaatcaat
     5581 tgctcttcac ctggaatgtt tagcttacgc caacctgcac cagtagcaat aatgactgtt
     5641 ttactttcta agatagcacc gttttctaac gtaactttaa ttgcttcgtc agtcttttcg
     5701 atatctgtag cacgtatacc tgtcattgca tcaatgtcat attgatcaat gtgcgctgct
     5761 aagttagaag aaaattcaga accagttgtt tctttaacag taatgaagtt ctcaatacca
     5821 gcagtatcat taacttggcc accgatacga tcagcaacta taccagtacg taaaccttta
     5881 cgtgctgtgt aaatcgctgc actaccacta gcaggaccac caccaacgat taagacatca
     5941 taaggttctt tattttcaaa ctcagatgca tctgccgtac tgcctagttt cgaaagaata
     6001 tcttggattg tcatacgacc attgccaaat tcttcgccat ttaaaaagac agcagggact
     6061 gccatgatgt tttcagattc ttcacggaac actgcaccat caatcataga atgcgtgatg
     6121 ttagggttga tcacactcat taagttaagt gcttgaacga catcaggaca tttttgacac
     6181 gttaaactaa tgaatgtttc aaaatggaat gaaccttcta agtttttaat ttggtcaatg
     6241 attgactgtt tttctttagg tgcacgacca ctaacctgta aaattgctaa aacaagtgag
     6301 ttaaactcgt gacctaatgg aatacctgca aatgttacac ctgtttcttc gccaggacga
     6361 ttgactgaga aacttggtgt acgttttaaa gacttttcag aaagtgaaag tctaggtgac
     6421 atatcagaaa tttctgttaa taattcttta agttccttag atttatcatc tgaaccaagg
     6481 ctggcaacga attcaacgtt gccctccatt agttctaata attgtttaag ttgttgtttt
     6541 aaatcagcat taagcatggt tgtaatgcct ccttagattt tacctactaa atctaaacca
     6601 ggttgcaatg ttttagcgcc ttcttcccat ttggctgggc atacttcgcc agggttttta
     6661 cgaacatatt gagctgcttt gattttgtga gctaatgtac tagcgtcacg gccaattccg
     6721 tcagcgttaa tttcagatgc ttgtacaaca ccgtctgggt cgataatgaa tgtaccacgt
     6781 tgagctaaac cagtagcttc atctaataca tcaaaattac gagtgattgt ttgtgatggg
     6841 tcaccaatca tagtgtaagt gattttgcta attgcatctg aatggtcatg ccatgctttg
     6901 tgtacgaagt gagtatcagt tgatactgag aatacattta cgcctaattt ttgtaattct
     6961 tcatattggt tttgtaagtc ttctaattca gttggacaaa cgaatgagaa gtcagcagga
     7021 tagaagcata ctacgctcca agaacctttt aaatcttctt gtgtaacttc tttaaattga
     7081 tctttttttg gatcgaaagc ttgcgctgta aatggtaaga tttctttgtt aattaatgac
     7141 ataaata
//
LOCUS       DAPIDM010000129         7101 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000129 DAPIDM010000000
VERSION     DAPIDM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.129"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..174)
                     /gene="hssR"
                     /locus_tag="RJE42_002208"
     CDS             complement(<1..174)
                     /gene="hssR"
                     /locus_tag="RJE42_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDV5877606.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGM"
     gene            310..1365
                     /locus_tag="RJE42_002209"
     CDS             310..1365
                     /locus_tag="RJE42_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877607.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPHKMNVQTLKLSNKEQD
                     VITMNDVKQQRLQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYSHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVNEKDLTD
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1365..2030
                     /locus_tag="RJE42_002210"
     CDS             1365..2030
                     /locus_tag="RJE42_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877608.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSKRATQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(2260..3228)
                     /locus_tag="RJE42_002211"
     CDS             complement(2260..3228)
                     /locus_tag="RJE42_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV5877609.1"
                     /translation="MLLNLLLIFILIVICPLILNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGMSMWLAILCLSLILTFICYLVWTVSYRHRSYTKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            3423..3878
                     /locus_tag="RJE42_002212"
     CDS             3423..3878
                     /locus_tag="RJE42_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5877610.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            4118..5500
                     /locus_tag="RJE42_002213"
     CDS             4118..5500
                     /locus_tag="RJE42_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877611.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVRLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLVNENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            5531..5658
                     /locus_tag="RJE42_002214"
                     /pseudo
     CDS             5531..5658
                     /locus_tag="RJE42_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5768..6976
                     /locus_tag="RJE42_002215"
     CDS             5768..6976
                     /locus_tag="RJE42_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDV5877612.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
BASE COUNT         2621 a          919 c         1282 g         2279 t
ORIGIN      
        1 cataccatcc atcataatat ctaccactgc aatatcgaca cgctgttttt ctaaaagttt
       61 taatgcagct tcaccacttg gttgtgtgta tgcatcaatg tgctctgttt gtaaatggct
      121 agctatataa tttaggattc gaggatcgtc atcgacaaca agacattgca ccatagctat
      181 aaactccctt atctttttca tttattatac atgtaaaata tttttgcgta aaaaaacaat
      241 tgttcatatt gagttcatat ttcaacctta tactgacgct aaagaagaaa tagggagaag
      301 tgaatcgata tgaaattagc gataaaagag attatgtttt acaaatttcg ttatatttta
      361 atcacattaa tcattctttt attaagtatt atggtgttat ttattagtgg tttagctcaa
      421 gggcttggta gggagaatat ttcgttattt gaacactttg ataatgatga atatgttgtt
      481 caaaaaatga aagagccgca aattgagaaa tcgcaactta gtgatacgca acagaatcaa
      541 attaaaaagg taattcatca agaaccacat aaaatgaatg ttcaaacatt gaaattatct
      601 aataaagaac aagatgtgat tacaatgaat gacgttaaac aacaacgctt acaacttaaa
      661 aaaggtgatt atcctaaaaa tgctcatgag gtcgctatta atgataagtt agctgcagac
      721 aacattagag tcggagatag attacatttt aaaaataatt caactagtta tagagtttct
      781 ggtattttaa acgacacaat gtattcgcat agttccattg tgctattgaa cgataacgga
      841 tttaatgcat tgaataaggt taatacggca ttttatccag tgaaaaattt aacgcaacaa
      901 caacgtgatg agcttaataa aataaatgac gttcaagttg tgaatgaaaa agatttaaca
      961 gataatattg cgagttatca agcagagcaa gcaccgttaa atatgatgat tgtgagtttg
     1021 tttgctatta cagcaatcgt tttaagtgca tttttctatg ttatgacgat tcaaaaaata
     1081 tcacaaattg gcattttgaa agcaattggt attaagacaa gacatttatt gagtgcgtta
     1141 gttttacaaa ttttaacact aacaataatt ggggtaggta ttgctgtgat catcatagta
     1201 ggactatcat ttatgatgcc tgtaacgatg cctttttact taacaacgca aaatatttta
     1261 ttgatggtgg ggatatttat attagtagcg attttaggtg cctcactatc attcatcaaa
     1321 ttatttaaag tggatcctat cgaagcaatt ggaggtgcag aataatggca ttagtcgttg
     1381 aagatatcgt caaaaatttc ggggaaggtt tgtctgaaac aaaagtttta aaaggtatta
     1441 attttgaagt ggaacaaggg gaatttgtca ttttaaatgg tgcctctggt tctgggaaaa
     1501 caacattgct aacgatatta ggcggattgt taagtcaaac gagtggtaca gtactttaca
     1561 atgatgcacc attgtttgat aaacagcatc gtcctagtga tttgcgattg gaagatattg
     1621 gttttatttt tcaatcttca catttagttc cttatttaaa agtgatagag caattgacac
     1681 tcgtaggcca agaagcggga atgaccaaac aacaaagttc aaaaagagca acacaacttt
     1741 tgaaaaatat tggtttagaa gatcgcttga atgtatatcc gcatcagtta tctggcggtg
     1801 aaaagcaacg tgttgcgatt atgagagcat ttatgaataa tccgaaaatc attttagcag
     1861 atgagcccac agcaagttta gatgccgata gagcaacgaa agttgttgag atgatacgtc
     1921 aacaaattaa agaacaacaa atgattggta ttatgattac acacgatcga agattatttg
     1981 aatatgcaga tcgagtgatt gaattagaag atggcaaaat aactgattag tggcttgtaa
     2041 agacgctaaa tgttaatgat ttaagacata gtagtataaa agttagataa cagaatacga
     2101 tttgggttta caaaaagcac gtcactatta tataaaatga tgacgtgctt tagtttattt
     2161 attttcaata taacaattgt ctaatcaagg attaacgaat atttaaagat agtttgacgc
     2221 aatattaaaa acaacctata ataatagttt atttgtgggt taactattat aaataaaagc
     2281 agcgtataga catataaacc aactacttga acaatataac gttaataaca atctatactg
     2341 atacattacg cctagataat ctttaatgag cacatgtaag aaaaagtgat atggtgtatg
     2401 gcttccgaca ccatcgatag ataaacctaa tttttgggct agtcgtaagg cgcgtaatac
     2461 atgaaactga cttgttacac aaacaatttt aactgcttca tgatacaaat tgttgatgat
     2521 ttgtttagaa tataaaaagt ttgtgtatgt atttatagag tgagattcca ttagtatatc
     2581 tgttttatca acaccatgtg caatcaaata acgttgcata gctaaagctt cagaaattgg
     2641 ttcgtctggt ccttgtccgc cagatacaat gatctttgtt gctgatgctt gttgttgata
     2701 gatatcaagt gcacggtcta aacgcgctgc aagcattggt gtgacaaatt cggtaaaaat
     2761 accagcacct aacacaatta tgatatcgac ttctttggtg tatgatctat gtctatatga
     2821 tactgtccaa acgagataac aaataaaggt tagtatcagg gaaaggcata atatagctaa
     2881 ccacatagac atacctttca caataggtga ctgaatcgta cttataaata gaagtgctga
     2941 tgtgtagagt acaaatttat atgaaaaaga taataatttt ttaataaata agcgactaga
     3001 agtatgagaa aataaatatc tatgtttgaa tagcatgata atactgatta ttataaatgt
     3061 tacaaacata gaccaaggga aagtataggt catgatgcta tagatgagtg acaaaaatat
     3121 cgatatgaca actaagatgt agcatgttaa atttaacgtc agagtatagt tgagaattaa
     3181 cggacaaata acgataagta taaatattaa taataaattc aataacatac tgacacctcg
     3241 cttataataa atattaagta taaatgtaga tgatttaatt tattaaagca aggagaacgt
     3301 agcaacatgt aagtcttaat ttgttatatt ttatatgggt caatattttt gtgtttttta
     3361 gatgtgcgat acaatgattg ctgaggatac tttataaaaa tagtaattag ggagaaataa
     3421 ttatggtaaa acatttacaa gaccatattc aatttttaga gcagtttata aataacgtta
     3481 acgcattaac tgcaaaaatg ttgaaagatt tacaaaatga atatgaaatt tcattagagc
     3541 agtctaacgt attaggtatg ttaaataaag aacctttgac aattagtgaa atcacgcaaa
     3601 gacaaggtgt aaataaggcc gcagtaagcc gacgaattaa aaagttaatc gatgctaaat
     3661 tagttaagtt agataaacca aatttaaata ttgatcaacg tttgaaattc ataaccttaa
     3721 ctgacaaagg tagagcatat ttgaaagaac gtaatgcgat tatgacagat attgcgcaag
     3781 atattactaa tgatttaaat tctgaagata ttgaaaatgt aaggcaagta ttagaagtca
     3841 tcaatcatcg cattaaaaca tatagcaatc ataaataggt ttttgaaggt gttcaaatat
     3901 aagactaaat atgtatgtat tgaacacctt ctttttagtt ataattgaga gagtactttc
     3961 ataaataatg taataaggta caattctaaa gtaaagttaa tcatttcttg aattgtattc
     4021 ttgggataat atttgtaaaa taataatggt aaaataataa aataattttt atgatataca
     4081 tataacaatt gaagaggaac agaattgagg tgaaagtatg aaatcttgcc cgaagtgcgg
     4141 acaacaagca caagatgatg tacagatatg tacacaatgt ggacataaat ttgatagtcg
     4201 tcaagcatta tatagaaagt caaccgatga agatatacaa actaataaca tcaagatgag
     4261 aaaaatggtg ccttgggcaa ttggtttttt cattttaatc ttaattatta tattgttctt
     4321 tttactaaga aacttcaatt cacctgaagc gcaaactaaa atattagtga atgcgattga
     4381 gaataatgat aaacaaaaag tagcaacatt attaagtact aaagataata aagtagattc
     4441 tgaagaagcg aaagtataca ttaactatat caaagatgaa gttaggctta agcaatttgt
     4501 cagcgacctt aaaaatacgg tacataaatt gaataagagt aagaccagtg tagcttctta
     4561 tattcaaacc agatctggtc aaaatatatt acgtgtaagt aaaaatggca cgcgttatat
     4621 ctttttcgat aatatgagct ttactgcacc taccaagcaa ccaattgtta aaccgaaaga
     4681 aaaaacaaaa tatgagttta aatctggtgg taagaaaaag atggttatag ctgaagcaaa
     4741 taaagtgacg ccaataggta attttatacc ggggacatat agaattccag ctatgaaatc
     4801 aactgagaac ggtgattttg caggccattt aaaatttgat tttagacaaa gtaattctga
     4861 aacggtagat gttactgaag attttgaaga agcaaatata actgtaactt taaaaggcga
     4921 tacaaaatta aatgatagtt ctaaaaaagt aactataaat gaccgtgaaa tggcattttc
     4981 aagttccaaa acgtatggtc catatccaca aaataaagat attaccattt cagcttcagg
     5041 taaagcgaaa ggtaaaacat ttacaacaca gacgaagacg attaaagcca gcgatttaaa
     5101 atacaataca gagataactt tgaattttga cagtgaagat atcgaagatt atgttgaaaa
     5161 gaaagaaaaa gaagaaaaca gcttgaagaa caaattgata gaattctttg ctggatattc
     5221 tttagcgaat aatgctgcgt ttaatcagtc ggattttgat tttgtatcgt catatataaa
     5281 aaaaggatca tctttttatg atgatgtaaa gaagcgtgta tctaaaggaa gtttaatgat
     5341 gattagttca ccacaaatta tagatgccga aaaacatggt gataagatta ctgcaaccgt
     5401 aagattagta aatgaaaacg gtaagcaagt agataaagaa tatgagcttg aacaaggttc
     5461 gcaagaccgc ttgcaattaa tcaagacatc agaaaaatag agtataaaac ttaaataaga
     5521 tgcatgtgtc atggaataca aacaacatcc tttcaaagta tttacaatta gtgtgaatta
     5581 ctttgaaggg attgtttttt gtgaaaaaat tatggagtgc tgaggtttat atcattttat
     5641 accttttata agttgtaatg atgttcatga aacttgtgag taatgctaat atataaaaga
     5701 gcatatttaa aattagtttc tgttcgtgat agttataaac taatcataat ataaaggaga
     5761 tagtcatatg aatgatttaa cgcattttaa caagcgttca ccgatattta ttattatttt
     5821 aggatctttg actgccattg gagcattatc aatagatatg tttttgcctg gattaccaga
     5881 tattagacat gattttcaaa caacgacgtc taacgcacaa ttaacattat cgatgtttat
     5941 gattggttta gcgttcggta atttgtttgc tggtccaatt tcagattcaa ctgggcgcag
     6001 aaagccgctt attatagcta tgattatttt tacattagca agtttaggta ttgtttttgt
     6061 acataatatt tggctgatgg ttgcattaag atttcttcaa ggagtgacag gtggagcagc
     6121 agcagttatt tcaagagcta tagcaagtga tatgtatagt ggtaatgaac taactaagtt
     6181 tatggcatta ttgatgcttg ttaatggtat tgcaccagtt gttgcgccaa caataggggg
     6241 cataatttta aattattctg tctggcgtat ggtctttgtc attctgacta tctttggctt
     6301 cgtcatggtc ataggttctt tattaaaagt acctgaatca ttaacagtga caaatcgtga
     6361 gtcaagtagt ggattaaaga caatgtttaa aaacttcaaa atattattga agacgccacg
     6421 ttttgtatta ccaatgctca ttcaaggtat gacatttgta atattattta cttatatttc
     6481 tgcgtctccg tttattattc aaaaaattta tggcatgact gcaatacaat ttagttggat
     6541 gtttgcagga attggtatta cgctcattat atcaagtcaa cttactgggt accttgttga
     6601 ttttatagac tcacaaaaat tgatgagagg catgacgatg attcagatta tcggtgtaat
     6661 tttagtgacg attgtattgc ttaatcattg gaacttttgg attttagcga taggatttat
     6721 cattttaata gcaccagtaa caggtgttgc gacacttggt tttacaatag ctatggatga
     6781 aagtagtagt ggtagaggga gttcatcaag tttattaggt ttagtgcagt ttttatttgg
     6841 tggtattgca tcgccattag tgggggtaaa aggtgaagat aatcctatac cttacataat
     6901 tattattata gctacagcag ttatattaat tattttacag atttataata tgaaggtatt
     6961 taaaacaaat cgatgataaa catagtttct tctattataa tgaattgaaa aatttaaaaa
     7021 caaaacttga gctattcata gacctatgaa tttagcatca aaaataaaaa tgaggctggg
     7081 acataaatcc ctaaaaaaac a
//
LOCUS       DAPIDM010000130         6988 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000130 DAPIDM010000000
VERSION     DAPIDM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.130"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..372
                     /locus_tag="RJE42_002216"
     CDS             <1..372
                     /locus_tag="RJE42_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDV5877613.1"
                     /translation="FFIFTYLLIFIYFLILTSRKGSPIVVITLISIMLYFILKDENIV
                     EIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKELSHVPIFIYST
                     IWIIFNLYAVNILLKYTHLIN"
     gene            complement(452..1096)
                     /locus_tag="RJE42_002217"
     CDS             complement(452..1096)
                     /locus_tag="RJE42_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDV5877614.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            1218..1961
                     /locus_tag="RJE42_002218"
     CDS             1218..1961
                     /locus_tag="RJE42_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDV5877615.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            1955..2791
                     /locus_tag="RJE42_002219"
     CDS             1955..2791
                     /locus_tag="RJE42_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDV5877616.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            2788..3717
                     /locus_tag="RJE42_002220"
     CDS             2788..3717
                     /locus_tag="RJE42_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDV5877617.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(4809..6851)
                     /locus_tag="RJE42_002221"
     CDS             complement(4809..6851)
                     /locus_tag="RJE42_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDV5877618.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         2411 a         1180 c         1036 g         2361 t
ORIGIN      
        1 ttctttatat ttacctattt attgatattt atttattttt taattttaac atctagaaaa
       61 ggctcaccca ttgttgttat aactttaatt tctatcatgt tgtattttat attaaaagat
      121 gaaaatatag tagaaattca actacttgtc acaatgagtt atcaatatat tttaggtgta
      181 ttgttaggcg aagttctaca atctagttgg acgattgcta aattgacttt ccagcgtccg
      241 agtccacagt gggatggtag tgcacttaaa gaactcagtc atgtacctat cttcatttat
      301 agtacaatct ggattatctt caatctttat gcagtgaata ttttactcaa atatactcac
      361 ttgataaatt aactaataaa aaagaagcct aaagcaatta tccatatgtc tttatcagta
      421 caatcatgga tatctttatg gcttctttat tttatttttc agcaaacatg ttcattgcat
      481 tttcgtaact gacaataatt acattatcat tacgtttaat gataattact ttattcattt
      541 catctttcga tacaatttcc acttcattac caatagaaat atctttacta gacaaatata
      601 ttagcaaatc ggtcttatct ctcacacgtt tgattgtaac gatatcgcca ggttcataat
      661 tcaaaatcgt tgttatatat ttctctttat attcattatt tctaggaatc acaccgccgt
      721 gcgggcaagt ttctgggaaa tttaacaagc tatccagcct ttcaacaaat aaatcagaaa
      781 ttcgatgttc taaaatttct gcttcttgat gtacttcttc ccaattatat ttcaatattt
      841 ctattaaaaa taattctaat agtcgatgtc tcttaatgat atcaagcgta tgcgttaaac
      901 catcctctgt taatctaaca cctttgtacg gttttgtttc aacatagcct gctttttcaa
      961 gacgtcctac catttcactt acagatggag gcttaatatt taaaaattga gataagattt
     1021 tatttgtcac aaagttttta tcgccattat tcgtaaggat tgcctttaaa tagtcctctt
     1081 tttcttcagt taacatactt tcacctcaca tacattgtct atattatatc acgaatttac
     1141 ttgacatgat aaatattctc agtttattat acaattaatt aggttagcct aaacttttaa
     1201 ttaggaggta taaacgtttg ttagaaacaa aagatttaaa tctgttttta ggtaataagc
     1261 atgtacttaa aaacatttcc ttatcgatac cagtacgcgg cgaaataatt ggtatcatgg
     1321 gcccgaatgg tgctggtaaa tcttccctta tcaagtcttt aattggtgaa tttaatgcta
     1381 ccggtactaa attgttatat aacaaaccta tacaacaaca actgcaacat attacatata
     1441 ttccacaaaa agcacatatt gatttagatt ttcctataag tgtggaacaa gtgattttat
     1501 caggttgcta caaagaaatt ggatggttta gacgacctaa taaatcagca agggataaac
     1561 tcaaacagtt attaagcgat ttagaattag aatctttacg tcatcgacaa atttcagaat
     1621 taagtggtgg acaattacaa cgtgtgctag tagcaagagc attgatgtcc gaaagtgaag
     1681 tttattttct agatgagccg tttgtcggaa ttgattttag tagcgaaaaa ttaatcatga
     1741 caaaaatcga gaacttaaaa caacaaggaa aacttattct tatcatccac catgatctat
     1801 caaaagcaaa gcaatacttt gatcgcatta ttctattaaa tcaaacatta cgatactttg
     1861 gtgatagtga agaggctatg agtgtcactc gcttaaacga aacatttatg agtagcactg
     1921 actgtagtga ccctagtcaa aggagcaata taacatgtta gagtttgtcg aacatttatt
     1981 tacatatcaa ttcttgaatc gagcattgat aacttcaatt attgtaggga tagtttgtgg
     2041 tacagttggt agtttaattg tattacgtgg tctttcatta atgggagatg caatgagtca
     2101 cgcagtatta cctggtgttg ccctatcatt cttatttggt attccaatgt ttgtaggcgc
     2161 acttataact ggtatgatcg caagtatttt tatcggttat atcacatcta gtagtaaaac
     2221 gaaacctgac gccgctattg gaattagttt taccgctttc ttagcttcgg ggattattat
     2281 tattagctta ataaacacta cgacagattt gtaccatatt ctgtttggta atttactggc
     2341 aatcacaaat agtgcatttt taacaactat tgtgattggt tcaatcgtcc ttattctaat
     2401 cattattttc tatcgtccat taatgatttc tacattcgat ccaacgttta gtagaatgag
     2461 tggtctaaat acgacgttat tacattactt tgtgatgttg ttactctcat tagtaacagt
     2521 agcaagtatt caaacggtag gtattattct tgtagttgca ttattaatta cacctgcatc
     2581 tacagcattt ttaattagta aaaaactata ctcaatgatg attatagcaa gtttaataag
     2641 tgtcataagt tcaattgtcg gactttatta cagctatatt tacaacattc ctagtggtgc
     2701 aacaatcgta ctttgtacgt ttgtaattta tatcatcaca ttatttttca caaaatttac
     2761 gaatagaaag aaacgaggaa gtttaacatg aaaaaattag tacctttatt attagcctta
     2821 ttacttctag ttgctgcatg tggtactggt ggtaaacaaa gcagtgataa gtcaaatggc
     2881 aaattaaaag tagtaacgac gaattcaatt ttatatgata tggctaaaaa tgttggtgga
     2941 gacaacgtcg atattcatag tattgtacct gttggtcaag atccacatga atatgaagtt
     3001 aaacctaaag atattaaaaa gttaactgac gctgacgtta ttttatacaa cggattaaat
     3061 ttagagactg gtaacggttg gtttgaaaaa gccttagaac aggctggtaa atcattaaaa
     3121 gataaaaaag ttatcgcagt atcaaaagat gttaaaccta tctatttaaa cggtgaagaa
     3181 ggcaacaaag ataaacaaga tccacacgca tggttaagtt tagataacgg tattaaatac
     3241 gtaaaaacaa ttcaacaaac atttatcgat aacgacaaaa aacataaagc agattatgaa
     3301 aagcaaggta acaaatacat tgctcaattg gaaaaattaa ataacgacag taaagacaaa
     3361 tttaatgaca ttccaaaaga acaacgtgcc atgattacaa gtgaaggtgc cttcaagtac
     3421 ttctcaaaac aatacggtat tacaccaggt tatatttggg aaattaacac tgaaaaacaa
     3481 ggtacaccag aacaaatgag acaagctatt gagtttgtta aaaagcacaa attaaaacac
     3541 ttattagtag aaacaagtgt tgataagaaa gcaatggaaa gtttatctga agaaacgaag
     3601 aaagatatct ttggtgaagt gtacacagat tcaatcggta aagaaggcac taaaggtgac
     3661 tcttactaca aaatgatgaa atcaaatatt gaaactgtac acggaagcat gaaataacac
     3721 gctgtgtttt aatgaagtaa gatgaattga tgttgatgca acctaaaata ttggtatctc
     3781 caatatttta ggctacacat caacataaca aagtcgaagg ctaatagtcc catatcgtgc
     3841 gttaaatata tattaccctc ctattaatat atataccgtt cccgatcgca cgatatggtg
     3901 gtattagaac ttctctttga gcgagagaga aaagctagaa gttcttatgc agttttgatt
     3961 aaactataaa catttatcac tttttaaatc aaagaggaag ctaatagttt tatgtggttt
     4021 taattaaact gcgaacagct gcctctcttt gagcgagaga gaaaagctag aagttcttat
     4081 gcagttttaa ttaaactata aacatttgtc actctttaaa tcaaagagca aagttgaaag
     4141 ctttatgtgg ttttaattaa actgtcgttc ccttcatctc ttttaaccac agagatgcgt
     4201 tagaagttct tctaatacaa tttatacaac gccattccct acacactctt ataaaagaga
     4261 ttcacgcgcg tcaataaatt gtattacata ctaactaaaa agcttttctt aatcgtacta
     4321 acgaagttag aggttcttat gtgattggac aactgttctc cctactagac ttttagcata
     4381 gctggttaaa agtactatta cagattgttc aattgtatgc ccctttcctt tgaacggaat
     4441 attaaaagct atgtgcagta acttttaata ggcataccac ttttgcatca agctataaat
     4501 aatcgcatac tttgatattt aatctggcct atcaaagtaa acatctatgg tttcctcccc
     4561 ccatagatcc ttaagccaca ctcatccgat gtcatatgag tgtggctatc ttttgtgaat
     4621 gcacttatct tcatctttaa atgacttcga ttaatctatc tcctccaatt tttaaaactt
     4681 atttcaatta aatctcaaat agtaataatt cgtatagttt aaaactatct attcaattcc
     4741 cctaaaataa aagggattga tgaaaatcca ttcagttttt catcaatccc ttttagcata
     4801 attacgaact acgtcatact atctacaata accatttcat cataattgat ggcttctctt
     4861 agttttaacg ctgtaccttc tgatacttca tctgtatcaa tcaactcacg taaaatgcga
     4921 cgttgttctc tcaatgcatt taatttaatt ttagtaatcg tatcttcact tgctgagttg
     4981 aagaaattag atggtgttaa gttttcaatt cgcattaaat aaccgtcaca aaccattcca
     5041 acttcaagtg tattatcttt tgttgtttcc ttagacaaac gactaaccac attataatgg
     5101 actaatttat taattttatt taattccatt aagttatccg taacactaag tgaagatgcc
     5161 gagttcactc tcgtttgtac tcttcgtttt aataaagcac ctcgtaataa tacaatcatt
     5221 cttcttatta atgatgcttg tctatatact tgtgttcttt cagcataacg catatagttt
     5281 tcaagtacac tattcgttat ttgtccttca tctactaaag actctaatgt ttttgtttct
     5341 acattaaaag caattttttg tagacgttct aattctttag agttttcatc atctttctct
     5401 acagttttta aaaatgctaa tttatcatga tattctttaa tcacgttacc atatttaaaa
     5461 cttgtttcga aagtagattt ttgatttaga taatcaataa cttgttctaa tatataaatt
     5521 ctagcaactt taaacgacat attaccaatt actgttttag gtgcaggttt cgttaataat
     5581 ggcaataata cttgcgcaac taccaaacta ataataacca taccagatgc aataaataat
     5641 aagtcgtttc tatacgtaaa agcatgatgt cctgctaaaa aatacggtaa cgttaatgcg
     5701 attgctaaag aaattgttcc atgcacacca cataacgtca taattaaagc gtataaactt
     5761 cgctttggtg gtttctcagt cgttggatta tcatcatcat ttttagtcat cattttttgg
     5821 aatggactga tggctaaata aaaataagga tataagacat aaacccaaac aaatctaaat
     5881 agatagacag ctaaagcaac aacaatagtg atgcctatta aaaagattaa attgtgcggt
     5941 tctgttttga taattttaat aataacttca ggtactaaaa atcctagtat cgaaaaaaca
     6001 aagccattta aaacataacc tagtatattc catgtatgat tgtaactcat ttgtagttgt
     6061 gtacgtactt gcataattct gtcacgttcg aaaccatgta caagtcctgc aactactgct
     6121 gcaatgattc ctgatgcgtg aaacaattca gcaattaaat acgtaacaaa tggtgttaac
     6181 aattgaataa ttgtaaacat attaatgttt tcatatcctc gacgcatcaa tgttaatcgg
     6241 aaccttacta atgccatacc tataagtaaa ccaaccactg cgcccccaat tgatgcaatt
     6301 aaaaacaact gaacagcatc aacaagtgaa aaagcacctg taactaatac tccaacagct
     6361 attttaaatg aaataatacc agcagcatca ttcaataatg actcaccttc aagaattgtc
     6421 attgctcctt ttggcaagac ctttccttta gtaattgctt gcactgctac tgcatcagta
     6481 ggacaaagaa tggcagcaat tgcaaatgct gctccaatag gtaaatctgg ccaaatccaa
     6541 tgaataaata aaccgacacc tatcacagta gtaatgacta atcctaatgc catcatcatc
     6601 actggcttaa tatatttcct taaatggact ctagaaacat taacaccttc tacaaataac
     6661 aaaggcgcaa tcattgttac cataaacaat tcagaatcaa aattaaattg aacagggatt
     6721 ggggtaataa atagtaacat gcccaagaaa atttgtataa atgctagggg tactttaggt
     6781 atgaaagtat ggacaaacga acttagtatc acaacagcta taaatataag aattgtttca
     6841 aatatttcca aactttcacc tctctaaaaa gtattgttta attgaaaaat aagtatcaca
     6901 tctcgttgta attatacttt agaggataaa ttgagttagc gaccacaaaa gcactttaat
     6961 atagatatat gtctacgatt gcagtact
//
LOCUS       DAPIDM010000131         6959 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000131 DAPIDM010000000
VERSION     DAPIDM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.131"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1232)
                     /locus_tag="RJE42_002222"
     CDS             complement(<1..1232)
                     /locus_tag="RJE42_002222"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV5877619.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGT
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGADVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALG"
     gene            complement(1698..2558)
                     /locus_tag="RJE42_002223"
     CDS             complement(1698..2558)
                     /locus_tag="RJE42_002223"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDV5877620.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            2776..3297
                     /locus_tag="RJE42_002224"
     CDS             2776..3297
                     /locus_tag="RJE42_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDV5877621.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(3406..5016)
                     /locus_tag="RJE42_002225"
     CDS             complement(3406..5016)
                     /locus_tag="RJE42_002225"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDV5877622.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(5352..5882)
                     /locus_tag="RJE42_002226"
     CDS             complement(5352..5882)
                     /locus_tag="RJE42_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDV5877623.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(5994..6854)
                     /locus_tag="RJE42_002227"
     CDS             complement(5994..6854)
                     /locus_tag="RJE42_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877624.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT         2171 a         1319 c          937 g         2532 t
ORIGIN      
        1 cctaaagctt ttaagtgttc tacaatatct gtataacctc tataaatatg tttcacattg
       61 taaattgtag ttacaccctc agcaattaaa ccagcaataa ttaaacaagc tcctgctctt
      121 aaatcgctag cataaacatc agcaccatgt aatgttgatg gttttatcgt tgctgtgcct
      181 tcgtcaactt caatatttgc acccatgcgc tttaattctt caacatgttt aaaacgttcc
      241 ggataaatcg tatcagttac aaatgaagga ccatttgcca taaataataa tggtgtaata
      301 ggctgttgta aatcagtagc aaaacctgga tatactagtg ttttaatatc aacaaattga
      361 tatggtgcat tattattgat gcgaattctt tcgtctctta catcaacatt cacacctaat
      421 tcactaaatt tagcagttaa tgtttctaca tgtttcggaa caatattatt taatataaca
      481 ttttctccac atgctgcagc gatacacata tatgtgcctg cttcaattct atcaggtata
      541 acttgatact cagaaccatg taattctttg acaccattga ttttaattgt tgatgtaccc
      601 gctcctttaa tattagctcc catacttgtt aagaagttag caacatcaac tacttccggt
      661 tctttagcag cattttcaat tacagtttgt cctgttgcat aaactgcagc tagcataata
      721 ttaattgttg cacctacact aaccatatct agaaaaatat gcgcaccctt taactcttta
      781 gcttcaattt tcattgatgt cgtacttgat tcatcaattt cagcacctaa agctttaaat
      841 cctttaatat gttgatcaat tggacgtggc ccaagtggac atcctcccgg taaaccaatc
      901 acacattttt taaatctacc taacatggtg cccatcatat agtatgaagc acgtaatgat
      961 tcaactttat tatttggtaa tgcagcattt tgtatttcag ttgtatcaac ttctaactcc
     1021 gtaccattta gtgatgcctt aatatttaaa tcttctaata aactcactaa agttttaaca
     1081 tcagagattt gcggtaaccc ttctaatttc acatgtcctt gagctaataa tgttgcagga
     1141 atgatagcta cagcactgtt tttcgcgcca ctgatattaa cttccccatt tagtgtgcgt
     1201 ccacctctta tttttattac ctcttgagcc atatcattta tctcctttgt ccattacttt
     1261 ttaatttttt gcattattag cattataaat taattttact ttatttgtaa aatagtttat
     1321 tttaatcgtt aaaatcttgt ctttctattt tcgattgatt aataacaaat cattcacctt
     1381 tactaatata tgctgttctt ccccacttga ccatggtcta aagtctgatt attcaactaa
     1441 atattgacgt tgttttgaca attatttagt tttatttcta tgatttgcac gtaaactttt
     1501 ttcataaatt gctcattttc cttataatta tatcacaatc aattttgata tatttcacca
     1561 tttttacgct ttcattttaa aatcaaactg ttttatttat tacttataca acataaaaaa
     1621 gaagcaaaca ccattttgtc tgcttctttt aaaattaata tccctacgtt attaataact
     1681 taaaaactaa atattaatta tttagcgcgg tttgaagtac cgaactcttt aattttacct
     1741 ttaactgttt ctttgatagc ttcacgagct ggtcctaagt atttacgagg atcgtaaact
     1801 tctttgtcgt tatttaaaac gtcacgaacc gcttttgctg aagcgatttg gttttcagta
     1861 tttacgttaa ttttagctgt accaaatgga attgcttttt ggatatcttt agttgggata
     1921 ccagtaccac cgtgtaatac taatggtaaa ccagttgata aaccgatttc ttccatttct
     1981 ttgaatccta attttggttc acctttgtat ggaccatgaa ctgaacctaa tgctggcgct
     2041 aatgcatcga taccagtttt ttcaactagt tcttgacatt ctttaggatc agcataaatg
     2101 ataccgtctg ctacaacatc atcttcttgt ccgccaacag tacctaattc agcttctaca
     2161 gaaacacctt tttcgtgagc gtattcaaca acttttttag ttgttgctac gttttcttcg
     2221 aatgggctgt gtgaagcatc gatcattact gatgtgaaac cagcatcgat agcttcttta
     2281 catttttcaa agcttgaacc atggtctaaa tggattgcta caggaatagt gatgtttaag
     2341 tcatgcatta acccttcaac cattttaaca attgtgtaga aaccgctcat gtaacgagca
     2401 gcaccttcag aaacacctaa aattacaggt gcattttctt cttgtgacgc ttctaaaatt
     2461 gcttgagtga attctaggtt attaatattg tattgaccta ccgcataacc attttctttt
     2521 gcatcaatta acatttcttt cattgaaact aaaggcatga aagttcctcc ttgagtgctt
     2581 tcgcacgtat ttttcaaagt aattatatca aaaaaatagc gtcaatgcat tgaaatactg
     2641 cagattaaat gtgtattttt tgaaaaatgc gatagttata ctaaatgcaa acgtatacat
     2701 ttttatacaa tttaagccgt tgcataatat gtagtcatta attacaatat tttataagta
     2761 gattggagga taaagatgtc aaaaatctta aacacacaac taataggtat ctttaatagg
     2821 ctagaaaaac aatctttaga aattcaaatg gccgcccaat gtttaattca agcaattggt
     2881 ggcgaaggtt atgtttatgt aaaaggttat gatgatttac agtttttcga aagtttcatt
     2941 ctacatagtg atgagcgttt aaaatctagc cgaaaacttg acgctattaa agactttaaa
     3001 gaaattgact caactgatcg tgtattatta ttcgcaccat tttataatga tcaagttgca
     3061 ttagatattc aaaaacttat cgacttagat atcgatgtgg tcttaataag caacaaacct
     3121 aaaacagatg atttccctga tcatctcgta cattttatcg atttatctac accaagacct
     3181 atagtctata ctgaagatta cgataagatt gtccagccac atgcgatggc attgaattat
     3241 gtatactatg acatttatac acaaatgatt gaaatgacaa gagacttaga attataaaag
     3301 tcactattgt tatttaatac cttgtcacta acataatatt aaaattataa gaaaaaccgg
     3361 tactttcaat gtttaacaat gaaagtaccg gttttattag caaatttatt tattttgttg
     3421 atattttaat gaagcttcaa tgaatgattt aaaaatcggg tgcggacgat ttggtctaga
     3481 taagaattct gggtggaatt gacaagcaat aaagaaatca tttgtcggaa tctctaccat
     3541 ttctactaaa cgtccatctg gacttgtacc agaaattacc ataccatttg cttctaattg
     3601 ttctctatag tcattattaa attcataacg atgacgatgt ctttcttcaa tttccgcttt
     3661 accataaaca tcttgtgcca atgtgccttc tttaattgaa catggatata agcctaagcg
     3721 taatgtacca cctaaatctt cgatatcttt ttgttctggt aataaatcta taattgggta
     3781 tggtgttgct gggtctaatt cagctgaatg tgcgccttca aggcctaata cgtttcttga
     3841 aaattcaact gttgcaagtt gcattcctaa acagatacca aagaatggaa cattgttttc
     3901 tctagcatac ttaattgcac taattttacc ttcacttgca cggaaaccaa atccacctgg
     3961 tactaaaata ccgtcgacat ctgcaaggta ttcggccgca ttttcatctg taacttcact
     4021 tgaatcaatc catctaatat caatatcttt agcgaaagga tatccggcat gttttaatga
     4081 ttcaactaca gataagtatg catcttgtaa gctaacatac ttaccaacta aaccaattgt
     4141 aattttacca tctaaattat taacgatatc taacaactgt ttccattcat caagctgtgt
     4201 ttcatatttc gcgtttaatt gtaaacgttt aataacgata tcatccatat tttgttggct
     4261 taattgtaac ggaatttcgt ataaagagtc tgcatcacga cattcaataa cactttcttt
     4321 attaatgtca cagaataatg caattttatc ttttaaatct tgtgtcattt catattcagt
     4381 tcttacaacg attaagtctg gttgaatacc taagcctcgt aattctttaa cgctatgttg
     4441 tgttggtttc gttttcattt ctccagcagc tttaatataa ggcagtaatg tacagtgaac
     4501 atacataaca ttttctctac ctaaatcgct acgaatttga cgaatcgctt caataaacgg
     4561 taatgactca atatcacctg ttgtaccgcc aatttcagtg ataacaacgt ctgcattcgt
     4621 actttcccct gcaagtaata aacgttcttt aatttcattt gtgatatgcg gaataacttg
     4681 aactgttccg cctaagtaat caccacgacg ttcttttttc aatacgtgtg aatacacttt
     4741 accggctgtt acatttgaaa acttgtttaa attaatatca ataaatcttt cgtaatgtcc
     4801 taagtctagg tcagtttctg caccatcatc cgttacgaat acttcaccat gttgataagg
     4861 actcattgta cctgggtcaa catttaagta tggatcgaat ttttgaattg ttacatttag
     4921 acctctatct tttaataatc tacctagaga agatgctgta atacccttcc ctaatgatga
     4981 aactacgcca cctgttacaa aaataaattt tgtcatgtct tgacctccta attattaaag
     5041 ggaatgcttc ctcatcaatt gtaaaaatta cgtaaaacat aacattaaag caagctcatt
     5101 caagttgttt atttgtcgaa ttcgacaaaa ttaaattctg tttacttacc gtttccatac
     5161 tgctatgtca ctatgtatat catttacaac tttgattttt gcgttatcat ttcttgattt
     5221 actcaattaa ataaaaaaac gctccctctc acaaattctg taaggggagc gtataaattt
     5281 tatacacgtg tcctatttaa aggagcccga ataaaatttt atcatctttc aactaaaagt
     5341 caaacaaaaa attaatcgtt gaagtcttct tcgtcttcaa acacttcttc gtcttcgtct
     5401 agttcatctt catcttcttc aatgactata tccgaatgat tgatttcttc ttcaacctgc
     5461 tcatcttctg gatcatttag ttcttcttga tcatctgttt gagctggaat atcatcgtcg
     5521 tcatccattt catcttcgcc caataatttt aagttttgat cttcttcatc atctgcatcc
     5581 agaatatcga atttttgaat agttggtgcg attttctctt caatatcatc taccgaatac
     5641 caatcacgta atccccataa attttctcca acatttaaaa aacgaccatc tgtgtttaaa
     5701 tctgtgtaaa attgtacaac acgattttca atttcttcgt actcataatc acctaacgct
     5761 ctaaactcat cgatgatatc atataagttc atagtttcgc ctttatcatt caataatgta
     5821 taagccatat caataaatga tttttcatca accatttgtt ttgtataatc ttgaattttc
     5881 attgcccaat acttcctttc gaaggatata atctaaattt atacgtctga attcatttat
     5941 atatagtaac aaaactaaat tataaatgac aatatcatat ttcgcaatca ctgttagtaa
     6001 tttaaatatt tttcgaaaac gacataatca tcagtttgtt cgatttcaac gaaattacta
     6061 tttagacata atggtttaaa atcattattc tctccaaaca gaatcatcgt tatcacatta
     6121 aggttgtcat agttttttct taagtaagta ggcaattgtc gaagcgcact tgaaccaata
     6181 ccattaaagc gatatgattt atccacttca atagcagcaa tgttcatgcg ttcaccaatc
     6241 tgctccaatg caataaagcc aaccttttgg ccatcaatca ttaaattaat gatatgtaca
     6301 ttggtcactt ctgaacgact ttcttcttca agctttaatg acgaaacatg ttttgaatct
     6361 aagtccaagt aatatagtcg ttctttgcca attggtcctt cttctttagt cgctgtatgc
     6421 atgaatccag cacgttcgta taggttccaa gcattgtcat tttccgcatc tactactaga
     6481 tatagatgat taaaatcagg aaatacacct tgaacatatt gtggcaatga catcattatt
     6541 ttcgtgccat atccaaaacc ttgatatttt tcattaatcg ataatgaacg aatataaacg
     6601 acattttcag gtgtatcata accttcatgc tgatagtaac gatgtaatac aaaaaagccc
     6661 accacttcat ttttatcgtt tattgctacg ttagcaatcc tatcagcatc attaattgca
     6721 tcatcaataa cttctttagg taaagacgaa tatatttgtt gtctttcatt caagtcaaaa
     6781 tcattaattg ctgaacgata ttgttcatca aatgctttta aagtgatacc ttcaaaacaa
     6841 agttctttag ccatatattt aacccctatc tttttatata tgaagcatga atccccatat
     6901 tattttacct attatttcac tacgatacca ttatttgctc taagttaaat aaattactt
//
LOCUS       DAPIDM010000132         6872 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000132 DAPIDM010000000
VERSION     DAPIDM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.132"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            108..1046
                     /locus_tag="RJE42_002228"
     CDS             108..1046
                     /locus_tag="RJE42_002228"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDV5877625.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSQELLPQHSQLNTNKTKKKNTDNSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDSISKKHFKNREKAQKILKPL"
     gene            complement(1408..3810)
                     /locus_tag="RJE42_002229"
     CDS             complement(1408..3810)
                     /locus_tag="RJE42_002229"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV5877626.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKAPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELSAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVNQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3810..4868)
                     /gene="pheS"
                     /locus_tag="RJE42_002230"
     CDS             complement(3810..4868)
                     /gene="pheS"
                     /locus_tag="RJE42_002230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV5877627.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5249..5989)
                     /locus_tag="RJE42_002231"
     CDS             complement(5249..5989)
                     /locus_tag="RJE42_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDV5877628.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQR
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(6229..6336)
                     /locus_tag="RJE42_002232"
     CDS             complement(6229..6336)
                     /locus_tag="RJE42_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877629.1"
                     /translation="MVLLTLVTTIHDIDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(6373..6696)
                     /gene="isdG"
                     /locus_tag="RJE42_002233"
     CDS             complement(6373..6696)
                     /gene="isdG"
                     /locus_tag="RJE42_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDV5877630.1"
                     /translation="MKFMAENRLTLIKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(6715..>6872)
                     /locus_tag="RJE42_002234"
     CDS             complement(6715..>6872)
                     /locus_tag="RJE42_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDV5877631.1"
                     /translation="QFLDETKRKSMINSDVNVTVKDKIMTLSTCEDAYSETTKRIVVV
                     AKIIKVS"
BASE COUNT         2206 a         1321 c          947 g         2398 t
ORIGIN      
        1 cgcaattaat tctttacaac atatataaat gtttctgacg ttattatcat ttatgatatg
       61 attatttttg ctaatgataa tgaaattata tgaaaggatt tacacaaatg gcgaatatcg
      121 tttttaaatt gtcagataaa gacataacga cattaatgtc acgcatttca tttgatactg
      181 agaatttacc tcaaggtatg aaagcacgtg caaagtatca aaatacaact gtaaatattt
      241 accaatcagg taaagtcatg tttcaaggta atcatgcaga agctgtgtct caagaacttc
      301 taccgcagca ttctcaattg aatactaata aaactaaaaa gaaaaatacg gacaattctt
      361 ctcttgaaca gactttaatg tatgatcaat ttaactgtat tggtagtgat gaggctggaa
      421 gtggcgatta ctttggtccc ttgactgtat gtgctgcttt cgttaccaaa gagcatgtcc
      481 ctattttaaa aactttgggt gtagatgatt caaaaaaatt aaccgatact aaaattgtag
      541 aacttgctga gcaattggtt actttcattc cacattcact attaacacta cacaatgaaa
      601 aatataatat tcagcaagca aaaggctgga ctcaagtaaa aatgaaagct gttcttcata
      661 atgaagccat caaaaatgta cttgaaaaaa ttgattcgtc tcaattagat tatatcgtca
      721 ttgatcagtt cgcaaaacgc gaagtgtata gccattacgc attatccgat ataccacttc
      781 caaagaaaac aaaatttgaa acaaaaggag aatcgaagtc attagccatt gcagtggcaa
      841 gcataatttc tagatacgca tttgttacat atatggatca aatatcaaaa aatattaata
      901 tgacgattcc taaaggtgcc ggtgctaaag tcgatgtaat cgccgccaaa attataaaaa
      961 aatacggtct ttcacgttta gattccattt caaaaaaaca tttcaaaaat cgtgaaaaag
     1021 cccaaaaaat tttaaagcct ctatgattta taaatgaaat ggatcctaat aggaaatcct
     1081 attaggtaag tcatactaat ttgctttgct cataagtatg actttatatg cagttgtgca
     1141 aagcatcaac tgtaatccgc tgtgcctctt tgatttttaa tcaagataaa ttggatccta
     1201 atacgaaatc ctattaggta agtcatacta atttgctttg ctcataagta tgactttata
     1261 tgcagttgtg cgaagcatca actgtaatcc actgtgcctc tttgatttat aaaatgaatc
     1321 ggaacctaat tggtgaatcc aaaaataaaa ccccatgtta gggatatcaa tctacttcag
     1381 atatccttat acatggggtt taaatcatta tctaataaca gcaccttgtt caattaaagc
     1441 tgcttcaatt tccgcttgta cttttgaaac gcgttcatct gtcaatgttt cttctgtgtc
     1501 taaataattt aaacgtattg caattgattt tttacctttt tctaaatgtt cgccctgata
     1561 tacatcaaag acaagtgtat cttttaatat attgccaccg tgtgcatgaa tcgttgataa
     1621 taaatcagct gctggaatat tttgatttac ttctaatgca atgtcacgag acatgcctgg
     1681 gaatctcgga attggctggt aattaatgta acctaccgac acagccatta atgcatcaaa
     1741 attcaactca aaaacatacg tacgttttaa atcattatca gctgctaatg ttgggtgtaa
     1801 ttcaccaata aaaccaacaa ctttattctc taataagatt tcagctgtac gacctggatg
     1861 caatccatca atatcagcac gacgataact aaattcaagg tttaactttt cagatactcg
     1921 atccacgaca ccttttgcta aataaaaatc aactgtttct ttcttacctt gccattgatt
     1981 gactacataa tctccagtta aaataccact taaatattca acttgatctg gtagttcacc
     2041 ttcaccatta gcaaagaaga cattgccgat ttcaaataat tttacatctt tatttttacg
     2101 tgccacatta tatgatgccg cttcgattaa atgtggtaat aaactttgac gtaatgacgc
     2161 atgcgcttca ctcattggca tcaataaatc aattgtttga cgctgttgca tcgcaaatgc
     2221 agtagcatct tctttagaaa ctaacgaata cgtaatagct tggtctaatc cagcaccttc
     2281 taacacttct ttaaccattc tcgttttata ttggcgatca gttaactgac cactagtaac
     2341 tttttcgaag acaggtaatg ttgaaggaat atcatcgtaa ccataaatac gtgcaacttc
     2401 ttcaattaaa tcttctttaa ttgtaatatc tttgcgacgt gacggcactt gcactgtaat
     2461 aacatcatca tttatttctg tatcaaaccc taattgatta aaaatagtaa caatatcatt
     2521 ttgtgacaaa tcaaatccaa ttgtgcgatt aattttatca gcagtgatgt cgattggtgt
     2581 aataaatgca ctaagttctc ctgaagacac tctatctttt agcacttttc cgtttgcata
     2641 agtttgtaat aaataacatg cacggtcgac tgcttcatct acaaattcag tagctattcc
     2701 tttttcaaaa cgactagatg attcactgcg taaatttaaa cgtcttgatg tatgacgaat
     2761 tgaaactgga tcaaaaatag caccttcaat aactatattt gatgtatgtt ctttaacttc
     2821 tgaaaaatca ccacccataa caccagctaa tgcaattgga gtttgtccat tagtaatgac
     2881 aatatcgctc gttaataatt cacgttctgt atcatctaat gttgtcattt tttcgccttc
     2941 attagcttga cgaacaacaa tttgttgtga accaatcgca tcttgatcaa acatgtgcaa
     3001 tggttgaccg tattctaata acacataatt tgagatgtcg acaacattat taataggacg
     3061 tatacctgct tttattaagc gtgcttgcat ccaaattggc gaaggttcaa ttgtcacgtc
     3121 gtgaacaaca cgtgcactat aatatggtgc tttatcttca ttttcaattg tcacagtcag
     3181 ttcatcattt gcagataact caagctcatt tgatgttgtc tctggcttag tcatttttgt
     3241 attatataat gctgcaactt cataagcagt acctatcata cttaaagcat cagcacgatt
     3301 cggcgttaaa tcaaattcca ttacttgatc gtctaaatat aaagcttgta aggcatctgt
     3361 tcctggaact tgggattcac taaatacata aatgcctgat tcaaaacttt tcggtatata
     3421 gttacttgaa ataccaattt cttgtaacga acaaatcata ccttctgaac gttcaccgcg
     3481 taatttagca cgcttaattt taataccacc aggcaatctg ccacctactt tagcaacaat
     3541 gacatattgt cctgcatcaa cgttcggtgc accacatacg atttgtacag gttcgtcttc
     3601 tccgatatca acttggcaaa catttaattt atcagcatca ggatgtttct cttttgactt
     3661 aacgaagcca acaactaaat ttttgatatc ttttgtgtag tcaattaaat catccacttc
     3721 aatacctgtg cgcgtaatac gttctgccaa attacttaca gaatcatcga ttgttacata
     3781 ttctttcaac cattcatttg atatcaacat tacatgtcac ctctatcttc taccgcttta
     3841 aattgatcta aaaatctcac atcattagta tagaaatgac gaatatcttc tataccatat
     3901 ttcaacattg caatacggtc tggtcccata ccaaatgcaa atccagagta ctctgaagaa
     3961 tcaaaaccag ccatttctaa tacattagga tgtaccattc cagcacctaa aatctcaatc
     4021 catcctgtgt gtttacacac attacaacct tttcctttac atttaaaaca tgacacatca
     4081 acttctacag aaggttcagt gaatgggaag taacttggac gtaaacgaat ttcacgatca
     4141 gcaccaaata atttcttagc taacaattct aaagtacctt tcaaatcact cattttaacg
     4201 tttttatcaa caactaatcc ttcaatttgt gtaaattgat gactatgtgt cgcatcatca
     4261 gagtcacgac gatacacttt accagggcaa ataattttaa ctggaccttg accatgacgt
     4321 gattccatcg tacgtgcttg cactggtgat gtatgcgtac gtaataaaat ttcatccgta
     4381 atatagaaac tatcttgcat atcacgtgca gggtgtgatt taggtaaatt cagcatttcg
     4441 aagttataat gatcttgttc aacttcatat ccattgacaa tttcataacc taaacctaag
     4501 aataagtctt caatttcttc tattgtacgt gttaatggat gctttgaacc gatttcaata
     4561 tgacgacctg gtaatgatac atcaattgtt tcttcagcca attgcttatt taatttttct
     4621 ttaactaaca tctgttgtct ttcatctaat tcattttgaa ttgtttgacg caattcattc
     4681 actttttgac caaacgcagg tttctcttca ttcggcaaat ccttcatcaa tttcattagt
     4741 ccgctaactg accctttttt acctaagtat ttcactttaa cttcttgcag tgcacgttca
     4801 tcatttgctt cattaatatc tacaagcgct tgttgtttta actctgacat tgtttgttgt
     4861 tcagacatat gtcactgacc tccctattcg aactattcat tttctattta tcatatttgt
     4921 tgtttaacta aaaaaagact ttcatcctct taaaaaataa gggacgaaag tcatttccgt
     4981 ggtaccaccc aaatttatgc ctaagcatac acttgtttaa tattgataac ggattgtctt
     5041 tccgacttaa atctctttaa gtaaccaaaa aggtgaaaag actttaaata attggaataa
     5101 cttgcagtca cgattattct ccctaaaaca attatttata taaatgtcca tgccttttta
     5161 ttggtttata ttcaattgtg tatgcaatca cgtagaaatg ttctgttaaa caattaatta
     5221 taattatatc ggaaaacttt caacacggtc aacctttcaa atgataaagt aaaatactac
     5281 ctgcaatcgc tacatttaaa ctttcagctt taccataaat aggtatgatt aaattttgtg
     5341 tagtatgtgc taataattca ggattaacac cttctccctc attacctaat agtaatgcaa
     5401 aagaatcact tgaagtaact tctttatatg ccactgcgtt ttcaagtgct gtaccataaa
     5461 caggaccatt aaattgagta ataaacgtat cgagatcttg tgtcataact ggcaaatgga
     5521 aaacactacc ttgactcgct cgcaacactt tatcttgata aggatctgtc gtacccttct
     5581 ccattattac agcatccatt ccagcagcat ccgcagttct aattaacgtg cctaaatttc
     5641 caggatcttg aacacgatcg ataagcaata cttgttgtgc tttagaaata tcatattgtg
     5701 gcttctcaat gattgcgaaa aacccttgtg gtgtcactgt acctgataaa gattcagcaa
     5761 ctttcatgtt tatttcaaaa acttcttgcg cgtatgcgat aatttgctga tctaatcttg
     5821 ccggttcaat tgcaaataat tgtgtaatta caatcccacg ttgataagct tcttcaatta
     5881 aatgcacacc ttcaattaaa gctaaaccag ttttatccct ctcacgtttc ttttttagct
     5941 tgttcgcttg tttaattcta ttattttgtg cagaagtaat ttgttccata gatagctcct
     6001 cgctttattt ttaaaaataa aaatattaat cattaataag atgaaaacat ttgattgtat
     6061 agttaatatt aattaatcga ttttatcact catattattt caaattgtat aaatttcttt
     6121 tatcgatact actactataa atcatacgct ccgaaatatc attattaatt cttttcttct
     6181 tcaaaataaa tcaaaatcat atatttgatg attaatttca aagcacattc atatcaaact
     6241 acgttttagc aatttgttgt tagcatgttt gtgttcatta aaaaaacgac catcatcgat
     6301 atcatgtatg gtcgttacta aagttaacaa taccaattgt cataacaagt actgcaacct
     6361 ctttaaattc aattatttca tgtaactata gcctatatca tatgtaatta ctttgttatt
     6421 gataatcggg ctactttcat cttcattttt acttctaaca tgtttatgcg ctgctttaaa
     6481 gacatcagat tttaaccaat ccgtaaaagc ttgctttgat ttccaaactg ttaaaatttt
     6541 cacttcatca aaatcttctt gttctaaagt ttgtgtaaca aacatgccat caaagccttc
     6601 taatgtttca atcccatgtc tcgtgtaaaa tcgttctata atatcttttg ctgttccttt
     6661 tattaacgtc agcctatttt ctgccataaa tttcataatt atcctctttt ctgtttaact
     6721 taccttaatt atttttgcga caacaacaat tcttttcgtt gtttcactat atgcatcttc
     6781 gcacgttgat aaagtcatta ttttatcttt taccgttaca ttaacatctg aattaatcat
     6841 agatttacgt tttgtctcat ctaaaaattg tt
//
LOCUS       DAPIDM010000133         6850 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000133 DAPIDM010000000
VERSION     DAPIDM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.133"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            416..730
                     /locus_tag="RJE42_002235"
     CDS             416..730
                     /locus_tag="RJE42_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDV5877632.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            746..1132
                     /locus_tag="RJE42_002236"
     CDS             746..1132
                     /locus_tag="RJE42_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDV5877633.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1161..2546
                     /locus_tag="RJE42_002237"
     CDS             1161..2546
                     /locus_tag="RJE42_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV5877634.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2549..2701
                     /locus_tag="RJE42_002238"
     CDS             2549..2701
                     /locus_tag="RJE42_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV5877635.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2724..3929
                     /locus_tag="RJE42_002239"
     CDS             2724..3929
                     /locus_tag="RJE42_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDV5877636.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLQLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3972..4193
                     /locus_tag="RJE42_002240"
     CDS             3972..4193
                     /locus_tag="RJE42_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877637.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4310..4807
                     /locus_tag="RJE42_002241"
     CDS             4310..4807
                     /locus_tag="RJE42_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV5877638.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4782..5288
                     /locus_tag="RJE42_002242"
     CDS             4782..5288
                     /locus_tag="RJE42_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV5877639.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5272..>6850
                     /locus_tag="RJE42_002243"
     CDS             5272..>6850
                     /locus_tag="RJE42_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV5877640.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLEN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSD"
BASE COUNT         2189 a         1257 c         1458 g         1946 t
ORIGIN      
        1 atttctatca aataattaat agaaatcagc ttttttacat tgcctaagaa cttaatgtcc
       61 cagtccattt tttatgttta aaaatagcat aaaaatctag ttatccgcat aaaaactgga
      121 cttatcacac tttatcaagg tcaaaaccac tcaatttact actaatttac tacttatgaa
      181 tgagctttga tacgacgatt tatccttgaa aagtgaagat ataaagatac ttccaataaa
      241 atttgaatat ttaataggta gacacttcaa aaaatgaggt gtctattttt ttacccgatt
      301 ttgaaaggaa gtgaacttat gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc
      361 tttaagacca tcaaacattc attcagccaa taaaaaagaa aggataggta aaaatatgga
      421 acttaaattt gtgattccca acatggaaaa aacattcggc aatttagaat ttgctggcga
      481 ggataaagtc gttcagcgaa gaatcaacgg acggctaact gtcttatcaa gaagctataa
      541 tctctattct gatgttcaaa gagcagatga tattgtggtg gtgcttcctg ctgaagctgg
      601 cgaaaaacat ttcggctttg aggaacgtgt gaagttagtc aatccacgta ttaccgcaga
      661 gggctacaaa atcggcactc gtggttttac aaattacctt ttacatgctg acgacatgat
      721 aaaagaataa agaaagagag gaaaaatgat gagattagca aatggcattg tattagataa
      781 agacacgact tttggagaat tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa
      841 tgaagacggg tcggtttcag atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg
      901 acaaggacgc atgattcaag taagtattcc tgccagcgtg cctttgaaag agtttgatta
      961 taacgcacgg gtggaactta tcaatcccat tgcggacacc gttgctactg ccacctatca
     1021 aggagcagat gttgactggt atatcaaggc agacgatatt gtgctgacaa aggattctag
     1081 ttcattcaaa gctcaaccac aagcaaagaa agaaccgaca caagacaaat agtcgctagg
     1141 tagaaaggag actttttcgc atgaaacagc gtggtaaaag gattcgccca tctggtaaag
     1201 atttagtctt tcattttacg atagcgtcac tcctgcctgt tttcctgctg gttgtcggac
     1261 tgtttcatgt gaagacaatc cagcagatca actggcagga ttttaaccta tcacaagcag
     1321 ataagattga cattccctat ttaattatca gtttcagtgt cgcaattctt atctgcttgc
     1381 tggtagcgtt tgtattcaaa cgggttcgct atgatacggt taaacaactt taccaccgtc
     1441 aaaaactggc aaagatgata cttgaaaaca agtggtatga atctgaacag gtcaaaacag
     1501 agggtttctt taaagatagt gctggtcgta caaaggaaaa gataacctac ttccctaaaa
     1561 tgtattatcg acttaaaaat ggcttgatac agatacgggt ggaaatcacg ctgggaaaat
     1621 atcaagacca actcttacac ttggaaaaga aattagagag tggcttgtac tgtgagctga
     1681 cggataaaga gttaaaggat tcctatgtgg aatatacttt gctctatgac accatagcca
     1741 gtcgtatttc tattgatgaa gtagaagcta aagatggtaa acttcgctta atgaaaaacg
     1801 tatggtggga atatgataag ctccctcata tgttgattgc tggtggtaca ggtggcggta
     1861 aaacttactt tatactgaca ctgattgaag ccttgcttca tacagattca aaactgtata
     1921 ttcttgaccc gaaaaatgct gatcttgcgg acttaggttc tgtgatggca aatgtctact
     1981 atagaaaaga agacttgctt tcttgcattg aaacattcta tgaagaaatg atgaaacgta
     2041 gtgaggaaat gaagcagatg aagaactata agactggcaa aaattatgct tacttaggtc
     2101 tcccggcaca cttcttaatc tttgatgaat acgtcgcttt catggaaatg ctgggaacaa
     2161 aagaaaacac cgcagttatg aataagctga aacagattgt catgttaggt cgtcaagctg
     2221 gcttctttct aatactggct tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc
     2281 gtgatcagtt taatttcaga gtggctttag gtcgtatgtc tgaaatgggc tatggcatga
     2341 tgtttggcag tgacgtacaa aaggatttct tcttaaagcg aatcaaaggt cgtggctatg
     2401 ttgatgtagg aacaagtgtc atatcagagt tttatactcc ccttgtacca aaaggatatg
     2461 atttcttgga ggaaattaaa aagttatcca acagcagaca gtccacgcag gcgacgtgcg
     2521 aagcggaagt cgcaggtgtg gactgatctt gctggctggt gtggcaatag ccacgccagc
     2581 acttaacccc ccgtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat
     2641 tagaaaatcc tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta
     2701 aaagttgaca tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac
     2761 agcatttaaa agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga
     2821 ctggtattac aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg
     2881 atttacagca gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa
     2941 tgctgtttga ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa
     3001 acatcttaca actgcaactg tcctattttc ttcatgagga ctatggtttc tattcttatt
     3061 cagagcatta tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag
     3121 ttctggtgga attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac
     3181 aaagaagctg gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc
     3241 ttgaccttgc cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt
     3301 gccaacagga agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac
     3361 tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa
     3421 gtgaagttta tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc
     3481 ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct
     3541 attatgcagt ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta
     3601 tcaatcggta tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac
     3661 tgaatgaaga atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa
     3721 aaccagagcc ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga
     3781 ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc
     3841 tcgaccatgc gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag
     3901 aacaggacgt gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg
     3961 agaacatttt tatgaatttt ggacaaaacc tttataactg gtttctatca aacgctcaat
     4021 cactggtgct tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta
     4081 gcaaactgat tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg
     4141 ctggagtaaa agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac
     4201 cgttcttttg tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac
     4261 gaagtgcggt agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat
     4321 gcaagtctat attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt
     4381 tacctttccc attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga
     4441 ggaatacgcc attcatgact acgagttacc ctttacggtt gacgaataca cttccattgg
     4501 cgaactcaat cgactatggg aaatggtatc ggaattaccc gaagaattac aatcggagct
     4561 atctgctctg ctcactcatt tttcaagcat tgaagaacta agcgaacatc aagaggatat
     4621 tatcattcat tccgattgtg atgatatgta tgacgtggca cgctactaca ttgaagaaac
     4681 gggtgcttta ggcgaagtac cagctagtct tcaaaactat attgattatc aagcctatgg
     4741 tcgggattta gacctttcag gaacgtttat ctcaaccaat catgggattt ttgaaatcgt
     4801 ctattaaatc tgtcggtaca ttactactgg cagattttct attttacggg gtggctcaat
     4861 cagctacccc tattttttat gaaaggattg attacatgaa gaaaatacga agctatacca
     4921 gtatctggtc tgtggaaaag gtactgtatt ctatcaatga ttttagactt ccgtttccca
     4981 taacctttac gcaaatgaca tggtttgtcg tgtcactctt tgcagtgatg atacttggca
     5041 acttgccccc tctttccatg atagagggag catttctcaa atactttggg attcctgtgg
     5101 ctttcacatg gtttatgtct acaaaaactt ttgatggtaa aaagccttat ggatttttga
     5161 agtctgtcat tgcttatgca ctgcgaccaa agctgaccta tgcaggaaaa aaagtaacgc
     5221 ttggcagaaa ccagccacaa gaagccatta cagcagttag gagtgaattt tatggcatat
     5281 ccaattaaat acattgaaaa caatctcgtc tggaataaag acggggaatg ttatgcttac
     5341 tatgagcttg ttccttacaa ttactcattt ctaagtccag aacagaaaat acaagtgcat
     5401 gattctttca gacagcttat cgcacaaaat cgtgatggca aaattcatgc tttacaaatc
     5461 agtacagaat ccagcatacg ttctgcacaa gagcgttcca aaaatgaagt cactggcaag
     5521 ctcaaagcgg ttgcctatga caaaatcgac caacagacag acgctttaat atccatgatt
     5581 ggcgaaaatc aagtgaacta ccgtttcttt atcggcttta agttgcttct caacgatcag
     5641 gagttttcta tgaaaagtct taccgttgaa gcaaaaaatg ctttgtctga ttttgtctat
     5701 gatgtgaacc ataagctgat gggcgatttt gttagtatga gtaatgatga aatcctgcgt
     5761 tttcagaaga tggaaaagct cttagaaaat aaaatctctc gtcgtttcaa aatccgcagg
     5821 ttagataagg acgacttcgg ctatctgatt gaacaccttt acggacagac aggcactgcc
     5881 tatgaagagt atgagtacca tctatcaaag aaaaagctgg agaatgaaac gctgattaaa
     5941 tactatgact tgattaagcc tactcgctgt ttggtggaag aaaaacagcg atatttgaaa
     6001 atccagcagg aagatgaaac cgtctatgta gcttacttta ccattaacag cattgtcgga
     6061 gaactggact tcccgtcctc tgaaatcttc tactaccagc aacagcaatt tacattcccg
     6121 attgatacgt caatgaatgt ggaaattgta gcgaatcgta aagccctatc tactgtccgc
     6181 aataaaaaga aagaactgaa agacttggat aaccacgctt ggcaaagtga taatgaaacc
     6241 agctccaatg tggcggaagc tctggaaagt gtgaatgagc tggaaaccaa tttagaccaa
     6301 agcaaggaat ctatgtacaa gctgtcttat gtggtaaggg tatcagcaaa tgatcttgac
     6361 gaactcaaac gtcgttgtaa tgaagtgaaa gatttttatg acgatttaag cgtaaaactg
     6421 gtacgaccat ttggggatat gctcggctta catgaagaat ttttacctgc cagcaagcgt
     6481 tatatgaatg attatattca atacgtgacc tctgatttcc tcgctggttt aggttttggt
     6541 gctactcaaa tgctggggga aaatgagggg atttatgttg gctacagctt agatactgga
     6601 cgcaatgtct atctgaaacc tgctcttgcc agtcaagggg ttaagggttc agtaaccaat
     6661 gcgttagcgt cggcttttgt tggttcgctg ggtggtggta aatcctttgc gaataacctt
     6721 atcgtctatt atgcggtgct ttatggggca caagcagtga ttgtagaccc aaaagcagaa
     6781 cgtggcagat ggaaagaaac cttgccagag atttcccatg aaatcaatat cgtcactctg
     6841 acttctgatg
//
LOCUS       DAPIDM010000134         6776 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000134 DAPIDM010000000
VERSION     DAPIDM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.134"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            304..1365
                     /locus_tag="RJE42_002244"
     CDS             304..1365
                     /locus_tag="RJE42_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDV5877641.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1622..3079
                     /locus_tag="RJE42_002245"
     CDS             1622..3079
                     /locus_tag="RJE42_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDV5877642.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3072..3794
                     /locus_tag="RJE42_002246"
     CDS             3072..3794
                     /locus_tag="RJE42_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV5877643.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKRTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            3945..5072
                     /gene="queG"
                     /locus_tag="RJE42_002247"
     CDS             3945..5072
                     /gene="queG"
                     /locus_tag="RJE42_002247"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV5877644.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5077..5547
                     /gene="trmL"
                     /locus_tag="RJE42_002248"
     CDS             5077..5547
                     /gene="trmL"
                     /locus_tag="RJE42_002248"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV5877645.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5706..6305
                     /locus_tag="RJE42_002249"
     CDS             5706..6305
                     /locus_tag="RJE42_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDV5877646.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6330..6482
                     /locus_tag="RJE42_002250"
     CDS             6330..6482
                     /locus_tag="RJE42_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877647.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2426 a          881 c         1264 g         2205 t
ORIGIN      
        1 aacttttcta ataaaatatt tgtaaaagtg ctattttatt gtgctttata ccaagtcgat
       61 tcaatttatc tatcttaata gtaatatggc tattttaata tttacaattt attacattat
      121 tagtcacaag atgtaattag tagtatttta aaattaacat aacattgttt actgagtaga
      181 ttgttcaaat ttactttatt ccttttgtgg attgttatat ttagattaaa ttagccaact
      241 aatcattgtt aaagttaatt ttctataata tgatgagttt aatgttcaaa ttggaggtcg
      301 tttatgagta acgcaaagaa tgctgataat aaacagtttt tcagtaagat tttaaattct
      361 attggtgctg gtgttgttat tgcacttgtt ccgaatgctt tattaggtga agtattaaaa
      421 atatttaaaa gtggtaatga aattttagaa ttaacgtatc agttagtgat actaattcaa
      481 tcatttatgg catttattat tggggtttta gctgctcatc aatttaaatt taatggcgca
      541 ggtgctgcta tagtaggtac atcagctatg attggttcag gtgctgttgt atatagcaat
      601 aatagtttta tgttaaaagg tataggtgac attataaata cgagtttggt tgttattatt
      661 gcatgtctta tatatatggt attacaaaac aaacttggat cttttgaatt aattatttta
      721 ccagttttag tacctatcgt tagtgggggt atcggtctaa tcacattacc ttacatacga
      781 aaaattactc aagcaattgg aaatgtaatt cattctttta cagacttaaa tccgttgtta
      841 atgtctatac ttattagtgt ggcattttca ttattaatgg taacaccaat atcgttagta
      901 gcgattgcaa ctgcgattag tttaaacgga ctaggaagtg gagcagcaaa cctaggtatt
      961 gtggctgcgt gtgttacttt cttatttggc tcattgcgtg tcaattctat tggtgtaaat
     1021 gcagttttac tcataggagc tgcaaaaatg atgatcccag tttacttgaa aaatttaatt
     1081 atttcgatac cattgactat aaatggaata attactggaa tcattgctta cgttcttcag
     1141 gttaaaggaa caccattgtc tgctggattt ggatatacag gattagttgg cccaataaat
     1201 gcatttaacc gaatgtcagg tgatccaaca atgaatatta ttttattagc tcttggttat
     1261 tttgtcatac catttgtatc ggcgtttatt gttcatgaat tgtgtaaaaa attcatccct
     1321 atatattcta atgatattta taaatttgaa gttccaaaac aataatttat agcttgatga
     1381 caagaataag ttttaatata tgataaatat aggcgttact gtcaataaga tattgatagt
     1441 agcgcttttt ttgattgatt ttaaaatgga agaattgttc acgtattagg cgatttttta
     1501 aataaagtct tttaaaaaga agcttttctt tatattgata aaaatacatt tataaaacac
     1561 tattgcatca aaatgtatta atatgtataa ttatgaatat taattaggga gggctgtata
     1621 aatgaagtgt ttaattaggt ttatattagt attagggttg ttaattagca gtgctatggt
     1681 gtatatcaat cccacagcac atgctgaaca agatcaaaca tgggagaaga ttaaagaacg
     1741 cggtgaactt agagtgggtc tttctgcaga ttatgctcca atggaatttg agcatacagt
     1801 taatggtaag actgagtatg caggtgtaga tattgattta gctaaaaaaa ttgcgaaaga
     1861 taataattta aaattaaaaa tcgtcaatat gtcatttgat agtttgttag gagctcttaa
     1921 aactggaaaa attgatatta ttatttccgg aatgacttca acgcctgaac gtaagaagca
     1981 agttgatttt tcagattcat atatgatgac taaaaatatc atgcttgtaa agaaagataa
     2041 agttaatgaa tataaagata tcaaagactt taataataaa aaagtagggg cacaaaaggg
     2101 aactgaacaa gaaaaaatcg ctcaaaccga aattgaaaat gcatctatta cttcattaag
     2161 ccgtttacca gacgttatat tagctctgaa aagtggaaag gttgaaggtg ctgtagttga
     2221 aaaacctgta gcagaagcat atttaaaaca aaatcctaaa ttaggaattt ccaatgtgaa
     2281 atttaatgaa gaagaaaaag atacagtgat agcagtgcca aaagattcac caaaattatt
     2341 gtcacaaatt aataaaacga ttaaagaggt taaagataaa ggattaatcg ataaatatat
     2401 gactaatgct gcaaatgcga tgaatgatga cagtggtttt atttctaaat atggtagttt
     2461 tttcttgaaa ggaattaaga ttacaatatt aatttcactt atcggtgtag cattaggttc
     2521 tattttaggt gcattcgttg cgttaatgaa attaagtaaa ataaaaatta tttcatggat
     2581 tgcctctatc tacatcgaaa tattaagagg aactccaatg ttagttcaag tatttatcgt
     2641 attctttggt ataacggctg cattaggact agacatttca gctcttgttt gtggaacaat
     2701 tgcattagtt attaattcct cagcttacat tgctgaaatt attcgtgcag gtataaatgc
     2761 tgttgataaa ggccaaatgg aagccgcacg tagtctaggt ttgaattata gacaaacgat
     2821 gaaaagtgta attatgccac aagcaattaa aaacatttta ccagctttag gtaatgaatt
     2881 tgtcacttta attaaagaat catctattgt gtcaacaatt ggagttggcg aaattatgtt
     2941 taatgcacaa gtggttcaag gtatttcatt tgacccattc acaccattac tagtggcagc
     3001 agcattatac tttgtcttaa catttgtact tacacgcatt atgaacatga ttgaagggag
     3061 attgaatgcc agtgattaaa ataaacaatc ttaataaagt ttttggagat aatgaagttt
     3121 taaaagatat caatcttgaa atcaatcaag gggaagtagt agcaataata ggtccatctg
     3181 gtagtggtaa aagtacattg ttaagatgta tgaatttatt agaagtaccc actaaaggtc
     3241 aagtgatttt tgaaggcaat gacttaacgg aaaaaaggac acaagtagat aaactacgcc
     3301 aaaaaatggg tatggtattt caaaacttca acctatttcc acataaaaaa gttgtcgata
     3361 atattatttt agctcctaaa ttattaaaga aagataataa cgatgaatta cataaggaag
     3421 cattgtcgtt attagataaa gtgggattaa aagaaaaagc agatgtatat ccgaatcaat
     3481 tatcaggtgg tcaaaaacaa agggtagcaa ttgcaagagc tttagcaatg catccagatg
     3541 ttattttatt cgatgaacca acttcagcat tagatcctga ggtagttggt gatgtattaa
     3601 aagtaatgaa agacctagcc aaagaaggta tgaccatggt ggttgtgaca catgaaatgg
     3661 gatttgccaa agatgtaagt gacaaagtca tatttatggc agatggcgtt gtcgtagagt
     3721 caggcacacc agtcgaaata tttgaacaac cacaacatga aagaacacaa aatttcttag
     3781 caagagtatt ataacaacct aacgaagctt gaatatatga tgcgcaccac aaagttatat
     3841 catatattca agctttttat tattaaaatt atgtgaaatg aaacctcaat atgaataatg
     3901 gttgtgttaa aataacatag tattatttta tggggggcat acgattggat acaaagcagt
     3961 taaagcaaga tatcattgac tatgcatata caattggtat cgacagtatt ggatttacta
     4021 ctgccgatcc ttttgatgaa ttgaagcaaa agctagaagc atatcatgca aatggttatg
     4081 cctcaggatt tgaagaatct gatattgctt tacgaacgga gcctaaatta tccttaccaa
     4141 cagcaaggtc aatcatagca attgcagttg gttatcctaa caaactgaaa ggtgcaccta
     4201 agagtgttag aggtgatcga agaggcttat ttgctagagc atcgtggggt caagattatc
     4261 atacaattat gcgtaaacga ttagacatgt tagctgcatt tattgaatct aaagttccag
     4321 atgttgaaat caaatctatg gtagatacgg gtgtattatc agatagggca gtagcagaac
     4381 gtgcaggttt aggctttgtt ggtcgtaatg gctttgtcat caatcctaaa ctaggaacat
     4441 ggacatacct cggtgaaatg ttagtcagta taccttttga acctgatgat ccattattag
     4501 atagctgtgg tgattgtaca atttgtgttg atcgttgtcc aacaagtgca ttagttggaa
     4561 atggccaatt aaatagtcag aaatgcatta gttttttaac gcaaacaaaa ggctatatgc
     4621 ctgaccaata tcgttataaa attggaaata gattatatgg ttgcgatacg tgtcaacaag
     4681 tttgcccgaa aaatagaggt attaataccg aacaagatga catcattttg gaaccagaaa
     4741 ttttaaagcc aagattagta cctttactac gtatgtctaa taaagaattc aaacaaacat
     4801 atggtcacct tgcaggtgct tggcgtggta aaaagccgat acaacgaaat gctattttag
     4861 cattggcaca ttttaatgaa gtggatgcaa ttccagaatt gaaaaaagtt gcaacaacag
     4921 atgaaagacc gatgattcga gcaactgctt attgggctat tggtcaaatt cttggggaag
     4981 aagcaagaga ttttatcaat gcaaattatg atcaagagga tgcagaagtt cagaatgaaa
     5041 tgataaaagg attagacaca aggagagaat agtaatatga caaatcatat cgttttatat
     5101 caaccagaaa tcccagctaa tactggtaac attgcacgta cgtgtgcagg tacaaacaca
     5161 catttacatt taattaaacc tcttggattt agaactgatg ataaaatgtt aaaaagagcc
     5221 ggtttagatt attgggaatt tgtgaatatt acgtatcatg atagtattga agcatttttt
     5281 gaagccacaa atggcgagta ttacttatta acaaaatttg gtaaaaagac ttatagtgat
     5341 tttgattttt caaatcatga caaagatttt tacttcattt ttggaaaaga aacgacagga
     5401 ttaccagact gggtgaaaga gaaatatcaa gacacagcgt taagaattcc tatgagtgaa
     5461 catattcgtt cattaaattt atcaaatact gctgcgttgt taatttatga agcgttacgt
     5521 caacaagatt ttccaggatt gaattaaaat ttactcattt tcattataaa aatgatgctt
     5581 aagtgctata tttagagtat aagcatgtta aagttaaatg gtttgatata ttgaaagtta
     5641 gggtataata attattaata tcgccgtttt tatgtaaaat gcaaactaag aggagtgtag
     5701 aaatcatggc aatgaacttt aaagtctttg acaatagtca acttgtagca gaatatgctg
     5761 ctgatattat tagaaagcaa tttaacaata atcctactac aattgcaggt tttcatttag
     5821 atacagatca agcgccagta ctagatgaat taaagaaaaa tgtagaaaaa catgctgttg
     5881 attttagcca aataaatatt ttagattatg acgataaaaa atcatatttc gaagcgttag
     5941 gtgtaccagc aggtcaagtt tatccaattg cttatgaaaa agatgcaatc gaattaatcg
     6001 ctgataagat taaaactaaa gaaaataaag ggaaattaac attacaagtt gtttctatcg
     6061 atgaacaagg taagttaaat gttagtattc gtcaaggact aatggaagca agagaaattt
     6121 tcttagtagt gactggtgct aataaacgag atgtagttga aaaattatat caagaaaatg
     6181 gtaaaacaag ctttgaacca gccgatttaa aagcacatag aatggtaaat gttattcttg
     6241 ataaagaagc ggctgcaggt ttacccgaag atgttaaagc ttactttacg tcacgctttg
     6301 cttaattaaa aatctgtgag gtattgatta tgtctaaaga caaagatcca aaattaaatt
     6361 atcatgaaga agaaaacagt atggtaactg attttgaaga tttaaaagaa ttaggtaaag
     6421 aaatggaaca aatttctgat caaaatgatc aagaaaagaa ttctgaagaa gacagtcagt
     6481 aaagtattat aattgtgtaa gatacagcta tttatcatgt aaacaatgaa tagtatagaa
     6541 cctatatcaa ataggattga taatcttgaa tatgaagtta ggtagcattt tgttacctag
     6601 cttttttatt atattcatag aagctgactt tccgctagcc tctgtgtttg tggtccgcca
     6661 acttgcacgt tattgtaagc tgactttccg ccagcttctg agtttgaggc ccgcagtcaa
     6721 ctactgccaa tataactttg taaagcataa aacattgatt tgtgtagcag acatag
//
LOCUS       DAPIDM010000135         6537 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000135 DAPIDM010000000
VERSION     DAPIDM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.135"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            128..1726
                     /locus_tag="RJE42_002251"
     CDS             128..1726
                     /locus_tag="RJE42_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV5877648.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            2103..3581
                     /gene="mqo"
                     /locus_tag="RJE42_002252"
     CDS             2103..3581
                     /gene="mqo"
                     /locus_tag="RJE42_002252"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV5877649.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKNVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVEIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(3646..4101)
                     /locus_tag="RJE42_002253"
     CDS             complement(3646..4101)
                     /locus_tag="RJE42_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDV5877650.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINYLIIFTIIFIVVYTLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(4098..4541)
                     /locus_tag="RJE42_002254"
     CDS             complement(4098..4541)
                     /locus_tag="RJE42_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5877651.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIKKLLLEPNGLIRMYLKDAHYTYSSRRFLKSIKERLSI"
     gene            complement(4703..6076)
                     /gene="hssS"
                     /locus_tag="RJE42_002255"
     CDS             complement(4703..6076)
                     /gene="hssS"
                     /locus_tag="RJE42_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDV5877652.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTNDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSNNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNHSH"
     gene            complement(6069..>6537)
                     /gene="hssR"
                     /locus_tag="RJE42_002256"
     CDS             complement(6069..>6537)
                     /gene="hssR"
                     /locus_tag="RJE42_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDV5877653.1"
                     /translation="DYDIPVIMLTARDALSDKERAFISGTDDYVTKPFEVKELIFRIR
                     AVLRRYNIHSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFMLAARPKQIFTR
                     EQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGYKVENHV"
BASE COUNT         2149 a          961 c         1168 g         2259 t
ORIGIN      
        1 taattgtgaa aaaaatcaca ttaatctcac tgttctgtaa aatgttatcg ataacaaaat
       61 gctatagcca gagcatgatt ttaatgaagc aattacacta ctactttatg gaaagaagag
      121 tcagcctatg ttagtaaata cgtttaaccc atttgataat ttattattat cgtctttaat
      181 tgcagcaata ccgattgtac tgtttttact ttgtttaact gtttttaaaa tgaaaggtat
      241 ttatgcagcc attacaacac ttgttgtaac attactaatt gcaataccat ttttcaaatt
      301 gccagttggt atcgcttctg gtgcagtagt cgaaggtttc ttccaaggca tcattccgat
      361 tggctatatc gttatgatgg cggtattgtt atataaaatt actgttgaat ctggacaatt
      421 tttaacaatt caagatagta ttacaaatat ttcacaagac caacgtattc aagttttact
      481 tattggattt gcattcaacg catttttaga aggtgcagca ggatttggtg taccaattgc
      541 aatttgtgca cttttattaa cacaattagg gtttaatcca ttaaaagctg cgatgttatg
      601 tttagtcgca aatgcagcgt ctggtgcttt tggtgcgatt ggtatccctg taggtgttgt
      661 agaaacgttg aaattacctg gagatgtttc agtattaggt gtttctcaat cagcaacttt
      721 aacattggca atcataaatt tcattattcc attcttactt atctttatta ttgatggttt
      781 tagaggtgtt aaagaaacat taccagcaat tttagtagtt tcaatcactt atacacttac
      841 tcaaggatta ttaactgttt tcagcggacc tgaattagca gatattattc caccgttatt
      901 aacaatgtta gcattagcag tattctctaa aaaattccaa ccaaaacaca tttatcgtgt
      961 taataaagat gaagaaattg aacctgcaaa agcacattct gcaaaagcag tattacatgc
     1021 atggagtccg ttcattgtat taacagtcat tgtaatgatt tggagtgcgc cattctttaa
     1081 aaacttattc ttaccaaatg gtgcattatc atcattagta tttaaattca acttacctgg
     1141 aacagtcagc gaagttacgc ataaaccatt agtattgact ttaaatatta ttggacaaac
     1201 aggtacagct attttactaa ctattattat tacaatttta atgtctaaaa aggttaactt
     1261 taaagatgca ggtagattat tcggcgttac atttaaagaa ttatggttac cagttcttac
     1321 aatttgtttc atcttagcaa tttctaaaat cacaacttat ggtggtttaa gtgcagcaat
     1381 gggccaaggt attgctaaag caggtaatgt cttcccagtt ctatcaccaa tcttaggttg
     1441 gataggtgtg tttatgacag gatcagttgt aaataacaac tcattatttg caccaattca
     1501 agcttctgtt gcacaacaaa ttggaacaag tggttcactt cttgtatctg ctaatacagt
     1561 tggtggtgta gcggcaaaac taatttcacc acaatcaatt gcaattgcaa ctgcagcagt
     1621 aaaacaagtt ggtaaggaat cagaattatt aaaaatgaca ttgaaataca gtgtatgttt
     1681 actaatattc atctgtattt ggactttcat cttgtcatta ttataaataa acgtatttca
     1741 aaatataaat atacagaggg tgagatgttt tctaacatct catctttttt taatggatca
     1801 ttgatgaaag aagtttgaca ttataataat ggtagcgctt tatgttaaaa tgaagagtga
     1861 gtaatcagca atcaaattaa attggttgat agctgttaag gtttatggtt ttgtctttgt
     1921 gctatcgcac ataaagtata taattaaagt agtttcgtta ttataaaata ttaatataca
     1981 tagtagatag taatagagca tcaccatggg aacctgttga gacactgatt caaagtggta
     2041 ttaatatgtc gtatttctcg aacgttccat tattcatttt aaaaaggggg actgtatttg
     2101 ttatgacaac acaacatagc aaaacagatg tcatcttaat tggtggcggt attatgagtg
     2161 caacattagg aacattactt aaagaattat cacctgagaa aaatattaaa gtgtttgaaa
     2221 aattagcaca acctggcgaa gagagttcaa atgtatggaa taatgctggt acagggcatt
     2281 cagcactttg cgagttgaac tatacaaaag aaggtaagga tggcacagtt gattgtagta
     2341 aagcaattaa gataaatgag cagtaccaaa tttcaaagca gttttgggca tatttagtta
     2401 aaacaggaca attagataac ccagatcgct ttattcaagc ggtgccacac atgagttttg
     2461 tcattggcga agataacgta gcttttataa aaagtcgtgt tgcaacgtta aagaaaaatg
     2521 ttttattcga aaaaatgaaa ttatcgcaag atgaagaaga aatgaaatct tgggtaccgt
     2581 taatgattga agggcgtaag tctgatgaac caattgcttt aacttatgat gaaactggta
     2641 cagatgttaa ctttggtgcg ttaactgcaa agttatttga taatttagaa caacgtggtg
     2701 tggaaattca atataagcaa aatgtattag acatcaagaa acagaaatct ggagcatggc
     2761 tagttaaagt taaagattta gaaactaacg aaacgacaac atatgaatct gattttgtat
     2821 ttattggtgc tggcggtgcg agtttaccat tactccaaaa gactggaatt aaacaatcaa
     2881 aacatattgg tggtttcccg gtaagtggat tattcctgcg ctgtacaaat caagaagtta
     2941 ttgatcgtca tcatgctaaa gtgtacggaa aagcagcagt gggtgcgcca ccaatgtcag
     3001 tgccacactt agatacacgt tttgtagacg gcaagcgttc attgttattt ggtccatttg
     3061 caggtttctc acctaaattt ttaaaaacag gttcacatat ggatttaatt aaatcggtta
     3121 aaccaaataa tatcgtgacg atgttatctg caggtatcaa agaaatgagt cttacgaagt
     3181 atttagtgtc acaattgatg ttatctaatg atgagcgtat ggatgattta agagtctttt
     3241 tcccaaatgc taaaaatgaa gattgggaag tgattacagc agggcaacgt gtccaagtaa
     3301 tcaaggatac ggaggattct aaaggtaact tacaatttgg taccgaagtc attacgtcag
     3361 atgatggcac attagctgca ttacttggtg catcacctgg tgcgtcaaca gctgtagata
     3421 ttatgtttga tgttttacag cgatgctatc gtgatgaatt caaaggatgg gaaccaaaga
     3481 ttaaagaaat ggtgccgtca tttggttatc gcttaacaga tcatgaggat ttatatcaca
     3541 aaattaatga agaagtaact aagtatttac aagttaaata ataaacgaaa cggtaatgtc
     3601 ttttaaatgt gatagacatt accgtttttt agttgttcat aaaaatcatt ttaattgttt
     3661 cagttgctcg ttaatagtgt ctacgtagtt cttgttttta aagaattgaa taatccaaat
     3721 caatgtgtaa accacaataa agataattgt aaatatgatt aggtaattta ttgttagtgg
     3781 aaaccaaccg gcaagtattg ctaaaggtaa gaatccgaca tacgttgtta tgaaatgcac
     3841 tatagttgct ttagtaatgc cccaatctgt gtatttaaag ataaaatctc caaggaaaaa
     3901 gacgacgcct atgagtaacc ataaaataat agaaatcaac attacggtag tttctgtgaa
     3961 atgcgtataa tacaatatgc caatagttga ttgtgggttc agtggataat atttgccgtc
     4021 tgcaaataac atactaaaga acagtgaaag ggacaaacca atgattaagc taataaataa
     4081 tgagtttttc aaatttttca tattgataag cgctccttta tagattttaa aaaacgtcta
     4141 gaagaatagg tgtagtgtgc atctttaaga tacatacgta taagtccatt tggctctaat
     4201 aataattttt taatgtaata cttgttgacg atttctgatt tggaaatgcg aatgaaatgt
     4261 tgtggtaatt gtttttctag ttcataaagt cgtaatttta gtttgaactt ttgattgctt
     4321 gtaatggctg ttacattttt attaataact tggaatgtta taacatctga tacattaatg
     4381 atatggattt ctttgtctat gtatccgact aatgtatgtg atttgtctaa atcattgact
     4441 gcattaataa tactttgaac gtgatcattc attttaggtg catgtatatc aatataagat
     4501 tccgtctcat ttgcattgat aaataaattg agtttcatca taggttaatg cctccttcaa
     4561 aattattaaa ccataaacga ccatcgatat atttaaaatg tgttgaatgg tagaaattaa
     4621 atgttaagtg gctagaaagc actaatcaat ataaaagata cctcctgaaa taaaaacaga
     4681 aatttttttc aggaggtaga gattaatgtg aatgatttgg cagtgtaata gtaaaggtgg
     4741 ttacatactc gttactttgt gtgaattgga ttgtaccatg atgcaattca atgatggatt
     4801 ttgtaattgc gagacctaaa ccattactat tatcatgttt gctcacttta tagaaacgtt
     4861 caaataaacg cgcttcagct tgtggactaa ttggtgaacc atcattactt attgtgaaaa
     4921 tgatgttgtt gttactatgt tgcaaagcga tgtcaattgc tccaccaaca tctgtatatt
     4981 taatagcatt tattaataaa ttactcaatg cttgatgtaa caaacgttga tttcctagga
     5041 aatttattga ttctaggtca gctaaaatga ttaacgactt ttcatcagca gcaaattgtt
     5101 catgtcgaat gatatcttta atgagttgat cgacttgaat cttatcatcg aataataaat
     5161 gttggtgatt atctaattcg gataagagca acaattcagt tgttaatccg ctcagctgcg
     5221 ttgtaatggt ataaatatca tttatatatt gttgtcgtaa tgttttatca gaagtttgtt
     5281 gtaactcgct taataaatga tgaatatgtg ttaatggtgt tttgatttca tgagatacat
     5341 tttgtacaaa atgctgcctc atttgatcaa cttgtcccaa tgactctctc atcttattaa
     5401 agtgatactg taatgttcca atttcatctt tgcgtgtttg tttgataggt gtttcaaaat
     5461 caccatcaat taagcgttcg gtcgctagct ttaacttttt tactggacga atgattgaat
     5521 aggttgatgc gataactaat gaaatcgaga taaataataa caacattaac aagacggcta
     5581 agaatgttct aaactcgcta aatgtttctc caatatctgg acgcatgaat actgctattg
     5641 aaccgtcgtt tgtcttaaag ttaataccaa cagtattgtc tgttacattg tcgaaaaatc
     5701 cagttacgaa taatgcgaat ggtttatctt taataccatg gtaatcttta ttatttaaca
     5761 cgttgttaat agcattttgt gataaagtat cttctctaaa aggttcacca taaaatgtct
     5821 tatgaccttt ttgatcaacg gtcattattt ggtaattcat ttgccctaaa tgtttgaaat
     5881 attgttgaat gtgcgttggt ttagctgatt gttcatattg tcttgcttct ttaagcgtct
     5941 tcatgatttt cgcgtcatta gatgctttta aattataatg atagtaaaca tttgttaata
     6001 caaaacttat taatgcacta aataaaataa ccgtaatgga ataaatcgca attctagcat
     6061 agagtgtttt aaacatgatt ctccacctta tagccttgtc ctcttactgt ttcaattgta
     6121 agtgtggcat ttaatttttt taatctttgg cgtagtcgct taatatgaac gtcaactgtt
     6181 cgttcatctc cttcataatc atagccccaa attttttcta ttatctgttc acgagtaaag
     6241 atttgcttag gacgtgctgc aagcataaat aataattgaa attccttgtt cggtaacgtc
     6301 atcgttttat tagatacttg gagttccaaa taggattggt ttagcgttaa gttgccaatg
     6361 gtcatttctg aatttgaatg gatattatat cgacgtaata ccgcacgaat tctaaaaata
     6421 agttccttaa cctcaaaggg cttggttaca taatcgtcag taccacttat aaacgcacgc
     6481 tctttgtcac taagtgcatc ccgtgctgtt aacataataa ctggtatatc ataatca
//
LOCUS       DAPIDM010000136         6491 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000136 DAPIDM010000000
VERSION     DAPIDM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.136"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            174..1340
                     /gene="aroC"
                     /locus_tag="RJE42_002257"
     CDS             174..1340
                     /gene="aroC"
                     /locus_tag="RJE42_002257"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV5877654.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISDEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLEQLDIEIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDGIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEVAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1366..2430
                     /locus_tag="RJE42_002258"
     CDS             1366..2430
                     /locus_tag="RJE42_002258"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV5877655.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIKYIGTYLNQFDQSFLLIDEYVNQ
                     YFANKFDDILSYKNVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYYKIPHGHAVMVGIIYQFIVANTLFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2440..3738
                     /gene="aroA"
                     /locus_tag="RJE42_002259"
     CDS             2440..3738
                     /gene="aroA"
                     /locus_tag="RJE42_002259"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV5877656.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFRHFNIPIEAEGLSITTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIERELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3745..4989
                     /locus_tag="RJE42_002260"
     CDS             3745..4989
                     /locus_tag="RJE42_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877657.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQGALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIETGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5003..5578
                     /locus_tag="RJE42_002261"
     CDS             5003..5578
                     /locus_tag="RJE42_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDV5877658.1"
                     /translation="MSETLNQIKESFIEYLLFRYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKHNIKLMNTNEIFDYIANHNCAFDIQIQFA
                     NVSKREQRLDDLIVAQLTESPNYQTYLHDLNSMSIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            5568..6155
                     /locus_tag="RJE42_002262"
     CDS             5568..6155
                     /locus_tag="RJE42_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDV5877659.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     QFKIFVPDSPTAILFLVISISLILIRKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            6227..>6491
                     /locus_tag="RJE42_002263"
     CDS             6227..>6491
                     /locus_tag="RJE42_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDV5877660.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGT"
BASE COUNT         2305 a          908 c         1233 g         2045 t
ORIGIN      
        1 ttataatgaa tcttagttcg tttaatatta agataattct gacatttaaa aagagatata
       61 aatcaatttc ttaattgagc ttgaaaacaa acatttatga atgcacaatg aatatgataa
      121 gattaacaac atattataat gttatcgtgg aagtatgaaa ggagcgagtg tgtatgagat
      181 acctaacatc aggagaatca catggacctc aattaacagt tattgttgaa ggtgtacctg
      241 caaatttaga aattaaggtt gaggacatta ataaagaaat gtttaagcgt caaggcggtt
      301 acggacgtgg acgtcgtatg caaattgaaa aagatacagt ggagattgtt tcgggtgtaa
      361 gaaatggtta tacattaggt agccctatta caatggttgt tactaacgat gattttacac
      421 attggcgaaa aattatgggt gctgcgccaa taagcgacga agaacgagaa aatatgaaac
      481 gtacaattac gaagccaaga ccgggacatg cagatttagt tggcggtatg aaatataatc
      541 atcgtgactt acgaaatgta ttggaacgtt catctgccag agaaacagca gcacgtgtag
      601 cggtcggtgc actatgcaaa gttttattag aacaattaga tatcgaaata tacagtcgtg
      661 ttgttgagat aggtggcatt aaagataaag atttttatga ttcagaaaca tttaaagcaa
      721 accttgatcg aaatgacgtc cgtgtaattg atgatggtat cgcacaagca atgcgcgata
      781 aaattgatga agcgaaaaac gatggtgatt caataggggg cgtagttcaa gttgtagttg
      841 aaaatatgcc tgttggtgta ggtagttatg tacattatga tcgtaaatta gatggtagaa
      901 tagcacaggg tgtcgttagt attaatgcat ttaaaggtgt aagttttgga gaaggattta
      961 aagcagctga aaagcctggt agcgaaattc aagacgaaat tctctacaat actgaattgg
     1021 gctattatcg tgggtcaaat cacttaggtg gtttagaagg cggtatgtca aatggaatgc
     1081 caataatcgt taatggtgta atgaaaccaa ttccaacgtt atataaacca ttaaattcag
     1141 tagacattaa tactaaagaa gactttaaag caacaattga acgttctgat agttgtgctg
     1201 ttcctgcagc aagtatcgtc tgcgaacatg tcgtagcatt tgaagttgca aaagcattat
     1261 tggaagaatt ccaatcaaat catattgagc aacttaaaca acaaattatt gagcgcagac
     1321 aattaaatat tgagttttaa caacaagaac aattgaggtg taatcatgaa attacaaaca
     1381 acataccctt caaacaatta tccaatatat gttgaacacg gtgcaatcaa gtatataggc
     1441 acatatttaa atcaatttga ccaaagtttc ttattaattg atgaatatgt aaatcaatat
     1501 tttgcgaata aatttgatga tattttatca tataaaaatg tgcataaagt cattattcca
     1561 gctggtgaaa agacgaaaac atttgagcaa tatcaagaaa cattagaata cattttgtcg
     1621 catcatgtaa cgcgaaatac agcgattata gctgttggtg gtggtgcaac aggagatttt
     1681 gcaggatttg tagcagcaac actattaaga ggtgtccatt ttatacaagt tcccacaacg
     1741 attttggcgc atgattctag tgttggcggt aaagtgggta ttaactcaaa gcaaggtaaa
     1801 aaccttatcg gtgcatttta tcgtccaact gctgtgattt atgatttaga ctttttaaag
     1861 acgttaccat ttgagcaaat attaagtggc tatgcagaag tttataagca tgcgttattg
     1921 aatggtgaat cagcgacgca agatatcgaa cagcacttta aagatagaga gatattacag
     1981 tcattaaatg gtatggataa atatattgct aaaggtattg aaacaaagtt ggatattgtt
     2041 gttgcagatg aaaaagaaca aggtgtacgt aaatttttaa atttaggtca tacatttggg
     2101 catgctgttg aatactatta taaaatacct catggtcatg cagtgatggt tggcattatc
     2161 tatcaattta tagttgcgaa tactttgttt gattctaagc atgatattaa tcattatatt
     2221 caatatttaa tacaactcgg ctatccttta gacatgataa ctgacttgga ttttgaaacg
     2281 ttataccaat atatgctaag tgataaaaag aatgataagc aaggtgtaca aatggtcttg
     2341 attagacaat ttggagatat cgttgtacaa catgttgatc aactaacatt acaacatgca
     2401 tgtgaacaat taaaaacata ttttaagtag gtgaatgaaa tggtaaatga acaaatcatt
     2461 gatatttcag gtccgttaaa gggcgaaata gaagtgccgg gcgataaatc aatgacacac
     2521 cgtgcaatca tgttggcgtc gctagctgaa ggtgtatcta ctatatataa gccactactt
     2581 ggcgaagatt gtcgtcgtac gatggatatt ttccgactgt taggtgtaga aatcaaagaa
     2641 gatgatgaaa aattagttgt gacttcccca ggatatcaat cttttaacac gccacatcaa
     2701 gttttgtata caggtaattc tggtacgaca acacgattat tggcaggttt gttaagtggt
     2761 ttaggtattg aaagtgtctt gtctggagat gtttccatag gtaaaaggcc gatggatcgt
     2821 gttttaagac cgttaaaaag tatgaatgca aatattgaag gaattgaaga caattacacc
     2881 ccattaatca ttaaaccgtc tgtaattaaa ggtattaatt acaagatgga agtggcaagt
     2941 gcacaagtga aaagtgccat cttattcgca agtctatttt ctaaggaacc gaccatcatt
     3001 aaggaattag atgtaagccg aaatcatact gagacgatgt tcagacattt taatattcca
     3061 attgaagcag aagggttatc aattactaca acccctgaag caattcgata cattaaacct
     3121 gcagattttc atgttcctgg cgatatttca tctgcagcgt tctttattgt tgcagcactt
     3181 atcacaccag gaagtgatgt aacaattcat aatgttggaa tcaatccaac acgttcaggt
     3241 attattgata ttgttgaaaa aatgggcggt aatattcaac ttttcaatca aacaactggt
     3301 gctgaaccta ctgcttctat tcgtattcaa tacacaccaa tgcttcaacc aataacaatc
     3361 gaaagagaat tagtgccaaa agcaattgat gaactgcctg taatagcatt actttgtaca
     3421 caagcagttg gcacgagtac aattaaagat gccgaggaat taaaagtaaa agaaacaaat
     3481 agaattgata caacggctga tatgttaaac ttgttagggt ttgaattaca accaactaat
     3541 gatggattga ttattcatcc gtcagaattt aaaacaaatg caacagtgga tagtttaacg
     3601 gatcatcgaa tagggatgat gcttgcagtt gcttctctac tttcaagcga acctgtcaaa
     3661 atcaaacaat ttgatgctgt aaatgtatca tttccaggat ttttaccaaa actaaagctt
     3721 ttagaaaatg agggataata taaaatggaa gatatctata aattaataga cgatatcaat
     3781 ctacaaaaac tagaaaattt agactctcgt gttaatgaag caataactac tgacaacgat
     3841 gacgcattat ttattctagg agagacactt tacaattttg gattaatgcc acaaggttta
     3901 gaagtattcc gcgtgttata tcacaaatat ccagacgaaa gtgaattgct gatttatttt
     3961 attgaaggtt taatgtctga aaatcaaact gacgaagcgt tagaatattt aagttatgtt
     4021 gaaccatcac ctgaaaagtt gatgttagaa gcagatttat atcaacaaat taatatgatg
     4081 gaagttgcta ttgataaatt acaaggagca cttgaactag agccaaatga tccaataatc
     4141 cattttgcgt tggctgaaat gttatattat gatggtcaat atttacgtgc tacctctgaa
     4201 tacgaaactg ttttagaaac tggtgaatat caagttaatg gtgtaaactt attctctcgt
     4261 atggcagatt gtagtttgca aagtggtaat tatagtgatg cgattcgctt atacgatgaa
     4321 attaatgaag atgaaatgac ttcagaagat tatctcaaaa aagccatttc ttacgataaa
     4381 aatgacatca ctcaagaagc aattaaaata atgactacat tactttctaa agaccctgat
     4441 tatattcaag gctacttgta tttacaatca ttatatgaaa atgaaaaaaa ttatccagat
     4501 gccattgaaa cgggtaaaga aggattacga ctaagtcaat tttataaaga gttaatgtat
     4561 acaactggat gtttggaaat agaacacggc gatgctaatg aaggtgtatt attgttaaaa
     4621 caagcattag aggttgataa tgcttaccaa gaacctttat tgattttaag cgatttatat
     4681 cgtaatgaag aagattatga agcaatcatt gaattattaa catatgtaga tgaagaagat
     4741 ttggatccta cattcatgtg gcatttagcg ttcgcctatg gtcaagaaga gcgagataaa
     4801 gaagctcaac atttctttga attagcatat ccaacgatga aaacaaatat tgattttatg
     4861 agtgattatt atttctattt aattgaaact ggtcaaaagg aacaagcaat tactatttta
     4921 aatcaattgt tggaactaga accaagtaat gaaaattggc atgacgaatt atcaagattg
     4981 caatcttaaa atggggtatt tgatgtcaga gacactcaat caaattaaag aaagttttat
     5041 tgaatattta ctttttcgat atcgatttaa gtcgcgtatt gcagtttggg tgcttaacta
     5101 tataaaagtg aatgaggcga aattggcaaa cattcatttt gtagatacaa aaattaatca
     5161 tcatgaaaca ttagaaattg cagaagtggg aagtcacgct tctgcaattc aatttactaa
     5221 gcataatata aagttaatga atacaaatga aatatttgat tatatcgcta atcataactg
     5281 tgcttttgat attcaaattc agttcgcaaa cgtgtcaaag cgtgagcaaa ggctagatga
     5341 tttaatagtt gctcaactaa cggaatcgcc caactatcaa acttatcttc atgatttaaa
     5401 tagtatgtct atagatcggc ataaacatgc gttattaata gattacttgc tacataatat
     5461 tgatttaagt ttacagatga atgaaaagca acgtttttat caactaacgc aaattttaaa
     5521 tacactcaag ctagtaaata aacataatca atttgaggac ttagcagatg acgattaata
     5581 cattttggca atatacactt tatcaacgtt cttggttaat gatgctactc atttgtaata
     5641 tattagggat gatttatggt tatatatggt atggtgaaca attaagtcat acgccgtggc
     5701 agtttaagat ttttgttcca gatagcccaa ctgcaatatt attcttagtt atttctatct
     5761 cattgatatt aatccgaaaa caaaatagta ttatcgatgc actggcattt gtaacattat
     5821 tcaaatatgg gatttgggca gttattatga atatattatt tattattgaa caaggtgata
     5881 ttacagtaaa tggcctggta ttaatgtttt cccacagtat aatggctgtt caggcaattt
     5941 acttctatcc taggtttaaa cgttctatga ttggaatcag tgtagcgatg acttgggtat
     6001 ttttgaatga ttatattgac tattttcacc ttcaatttcc atattatgat tttataacta
     6061 cacatgtgtg gcaaattggt gtattatcat gttgtctgag tgtttttgga cttttgcttt
     6121 atattgaatt aaacaaatta ttaaagtgta aatgatttga taaaaacggc aaatgacagt
     6181 aaaatattta taaaggagag tgagtttatt gtcttcaatt tttactatga taatttattt
     6241 cgtaatatta atggtgcttc ctttatgggc acaacacaag gttaaatcta actatgagaa
     6301 atattcacaa gttagatcaa ctagtggtaa aacgggtcgt gaagttgcat tagaaatttt
     6361 acatgcaaac ggtatttatg atgtcgaagt tgttaaaggt gaaggattct taacagatca
     6421 ttacgatcct aaaaagaaag ttgtttcact atctcctgca aactatgaca gaccatcagt
     6481 tgctggaact g
//
LOCUS       DAPIDM010000137         6341 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000137 DAPIDM010000000
VERSION     DAPIDM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.137"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            85..846
                     /gene="sufC"
                     /locus_tag="RJE42_002264"
     CDS             85..846
                     /gene="sufC"
                     /locus_tag="RJE42_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV5877661.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            945..2252
                     /gene="sufD"
                     /locus_tag="RJE42_002265"
     CDS             945..2252
                     /gene="sufD"
                     /locus_tag="RJE42_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV5877662.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2358..3608
                     /locus_tag="RJE42_002266"
     CDS             2358..3608
                     /locus_tag="RJE42_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV5877663.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSTIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            3598..4062
                     /locus_tag="RJE42_002267"
     CDS             3598..4062
                     /locus_tag="RJE42_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDV5877664.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4213..5610
                     /gene="sufB"
                     /locus_tag="RJE42_002268"
     CDS             4213..5610
                     /gene="sufB"
                     /locus_tag="RJE42_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV5877665.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            5797..5936
                     /locus_tag="RJE42_002269"
                     /pseudo
     CDS             5797..5936
                     /locus_tag="RJE42_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5942..6256)
                     /locus_tag="RJE42_002270"
     CDS             complement(5942..6256)
                     /locus_tag="RJE42_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877666.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSNDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         2292 a          896 c         1222 g         1931 t
ORIGIN      
        1 attattacaa agtctttttg actacaaatt aaaattatta taaactagtt aagaaaactt
       61 tatattttac ggagggaata taaaatggca tcaacattag aaatcaaaga cctacatgtg
      121 tctattgagg ataaagaaat cttaaaaggt gttaacttga caattaacac tgatgaaata
      181 catgcgatta tgggaccaaa cgggacaggt aaatcaactt tatcatctgc aattatggga
      241 cacccaagct atgaagtaac taaaggagaa gtacttttag acggtgtaaa cattttagaa
      301 ttagaagttg atgaaagagc aaaagcagga ttattcttgg caatgcagta tccatcagaa
      361 attacaggtg ttacaaatgc tgatttcatg cgttcagcaa tcaatgcgaa acgtgaagaa
      421 ggacaagaaa tcaacttaat gcaatttatt aagaaattag ataaaaatat ggattttcta
      481 gacatagata aagacatggc acaacgttat ttaaatgaag gtttctcagg tggagagaag
      541 aaacgtaacg aaatcttaca attaatgatg ttagaaccta agtttgcaat cttagatgaa
      601 atcgattcag gattagacat cgatgcatta aaagttgtat ctaaaggtat taaccaaatg
      661 cgtggggaaa actttggtgc attaatgatc acacactatc aacgattatt aaattacatt
      721 actcctgata aagtacatgt aatgtatgct ggtaaagtcg ttaaatctgg tggtccagaa
      781 ttagcaaaac gtcttgaaga agaaggatat gaatgggtta aagaagagtt cggttcagct
      841 gaataatctt aattaataca gtatccatga gatgttcatc tatatatgat gaaaatgaac
      901 atttatacga aatagtaaat ttcatcaagt aggaggaaaa agttatgaca actgatattt
      961 tgaacatttc tgaagaacaa cttgttgatt attctaaagc ccacaatgaa ccttcttgga
     1021 tgacagaatt acgtaaaaaa gctttgaaat taacagaaac tttagaaatg ccaaaacctg
     1081 ataaaacaaa attaagaaaa tgggattttg attcttttaa acaacacgat gtaaaaggtg
     1141 atgtttatca atctttatca caattacctg agtcagtaag agaaattatt gacgtagatc
     1201 attctaaaaa cttagtaatt caacataata atacgattgc gtacacacaa gttgatgata
     1261 atgcatcgaa agatggcgtt atcgttgaag gtttagcaga cgctcttatg aaccatagtg
     1321 atttagtaca aaagtacttt atgaaagatg cggtaacagt agatgaacat cgtatcacag
     1381 cgctacacac ggcattagtt aatggtggcg tatttgttta tgttcctaaa aatgtagttg
     1441 tagaacatcc agtacaatac gttgtgttgc acgacgacga aaatgcaagc ttttataacc
     1501 atgttatcat cgttactgaa gaaagcgccg aagtcacata tgttgaaaat tacttatcaa
     1561 atgcatctgg tgaaggaaat caattaaata ttatttctga agtgattgct ggtgcaaatt
     1621 caaatatcac atatggctca gtagactata tggataaagg ctttacaggt catatcattc
     1681 gacgtggtat tactgaagcg gatgcctcaa ttaattgggc attaggttta atgaatgagg
     1741 gtagccaaat tattgataat acaacaaatt tatttggtga tcgttcaact agttcactta
     1801 aatcagtagt tgtaggtaca ggcgaacaaa aaattaatct aacatctaaa atcgtacaat
     1861 atggtaaaga aacagatggt tatatcctta aacatggtgt tatgaaagaa catgcatcat
     1921 ctgtatttaa tggtatcggc tacattaagc atggtggaac taaatcaatt gctaatcagg
     1981 aatcacgtgt attaatgtta tctgaacatg ctcgtggtga cgcgaatcct attttattaa
     2041 ttgatgaaga tgatgtacaa gctggtcatg ctgcatcagt aggtcgtgtt gatccagatc
     2101 aactttacta tttaatgagt cgtggtattt ctcaaagaga agcggaacgt cttgttatac
     2161 atggtttctt agatccagta gtacgtgaat tacctatcga agacgttaaa cgtcaattga
     2221 gagaagtaat tgaacgcaaa gtttctaaat aatattttga aaataaaagt ttgtaataga
     2281 tatagactgt cgatattggt ataagactaa accaacgtca gtatttaaat gattaggatt
     2341 tttatttaag aaaggtcgtg aatgaagtgg ccgaacactc atttgacgtt aatgaagtaa
     2401 tcaaggattt tccgatatta gatcaaaaag tcaatggcaa acgtttagca tatcttgatt
     2461 caacagcgac aagtcaaacg cctgtgcaag tgttaaatgt tttagaggat tactacaagc
     2521 gttataattc aaacgttcat cgtggtgttc atacattagg atcattggca actgatggtt
     2581 atgaaaatgc acgtgaaacc gttcgtcgtt ttattaatgc gaagtatttt gaagaaatca
     2641 ttttcacacg cggaacaact gcatcgatta accttgtagc acatagttat ggtgatgcaa
     2701 atgttgaaga gggcgatgaa attgttgtca ctgaaatgga acatcatgcc aatattgttc
     2761 cttggcaaca gttagcaaag cgtaaaaatg cgacattgaa atttatacca atgacagctg
     2821 acggtgaatt aaacatcgaa gatattaagc aaacgattaa tgataaaaca aagatcgttg
     2881 ctattgcaca tatttctaat gtactcggta caattaatga tgttaaaacc attgcagaaa
     2941 tagctcatca acatggtgca attatcagtg ttgatggggc gcaagcagca ccacatatga
     3001 aacttgatat gcaagaaatg aatgctgatt tttatagttt tagtggacat aaaatgcttg
     3061 gaccaacagg tattggcgta ttatttggta aacgtgagtt actacaaaaa atggaaccga
     3121 ttgagttcgg tggcgacatg attgattttg taagtaagta tgatgcaaca tgggctgatt
     3181 tacctactaa atttgaggcg ggtactccat taattgctca agcaattggg cttgcagaag
     3241 ctattcgcta tttagaacgc ataggttttg atgcaattca taaatatgaa caagaattaa
     3301 cgatatatgc ttatgagcaa atgtctacaa ttgaaggaat tgaaatttat ggcccgccaa
     3361 aggatcgtcg tgcaggtgta ataacgttta atttacaaga tgtacatcca cacgatgttg
     3421 ctacagccgt agatacagaa ggtgtagcgg ttagagctgg gcatcattgt gcgcaaccgt
     3481 taatgaaatg gttaaatgtg tcttcaacag ctagagcgag tttttatata tacaacacga
     3541 aagaagacat tgatcagtta ataaatgcct tgaaacaaac gaaggagttt ttctcttatg
     3601 aattttaata atctagatca attatataga tctgtcatta tggatcatta taaaaatcct
     3661 agaaataaag gtgtattaga taacgggtct atgacagtag atatgaataa cccgacatgc
     3721 ggtgaccgta tacgactaac atttgatata gaagacggca ttataaaaga tgctaagttt
     3781 gaaggtgaag gttgttcgat ttcaatggca agtgcatcga tgatgacaca agctgttaaa
     3841 ggtcattcac ttggagaagc aatgcaaatg agccaagaat ttacgaaaat gatgcttggt
     3901 gaagactatg tgattacaga agaaatggga gatattgaag cattgcaagg tgtatcacaa
     3961 ttcccagctc gtattaaatg tgccacatta gcttggaaag cattggaaaa aggtactgtt
     4021 gctaaagaag gtaaagcaga aggtacgact gaagaagaat agaatgctgt taatcataga
     4081 tgattttgat attagacata taatagtaaa aaaattttta aatgatgtca tgtcattgtt
     4141 ataatatggt ttacatcatg aattaaaaac ttacgcacgc cgttgtaaat atatttttaa
     4201 ggagtgattg aaatggctaa aaaagcacct gatgttgggg attataaata tggattccac
     4261 gacgatgatg tgtccatttt cagatcagaa cgtggtttaa ctgagaatat cgttagagaa
     4321 atttctaaca tgaaaaatga gccggaatgg atgttagatt tccgtcttaa atcattaaaa
     4381 ttgttttata aaatgccaat gcctcaatgg ggtggcgact tatcagaatt gaatttcgat
     4441 gacattactt actatgtaaa gccttcagaa caagctgaac gttcatggga tgaagtacct
     4501 gaagaaatta aaagaacttt cgataaatta ggaattcctg aagctgaaca aaaatattta
     4561 gctggtgttt ctgctcaata tgaatctgaa gttgtttacc ataatatgga aaaagaactt
     4621 gaagaaaaag gtattatctt taaagatact gatagtgctt tacaagaaaa tgaagaatta
     4681 ttcaaaaaat actttgcttc tgtagtacct gcagcagata acaaatttgc ggcgttaaac
     4741 tcagcagtat ggtcaggtgg ttcgttcatt tatgtaccta aaaatatcaa actagatacg
     4801 ccactacaag cttatttccg tattaactct gagaacatgg gtcaatttga acgtacatta
     4861 atcattgctg atgaaggtgc ttctgtacat tacgtagaag gttgtactgc accagtttat
     4921 acaactagtt ctttacactc tgctgttgtg gaaatcattg tgcataaaga tgcgcacgtt
     4981 cgttatacta cgattcaaaa ctgggcgaac aatgtataca atttagttac gaaacgtact
     5041 tttgtttatg aaaacggaaa tatggaatgg gtagatggta acttaggttc taagttaacg
     5101 atgaaatatc caaactgtgt tcttttaggt gaaggtgcaa aaggtagtac attatctatt
     5161 gcatttgctg gtaaaggaca agttcaagat gccggtgcta aaatgattca taaagcgcca
     5221 aatacatctt caacgattgt ttctaaatct atttctaaaa atggtggtaa agttatttat
     5281 cgcggtattg ttcattttgg acgtaaagca aaaggcgctc gttcaaatat tgaatgtgat
     5341 acattaatct tagataacga atcaacatca gatacaattc catataatga agtattcaac
     5401 gatcaaatat cattagaaca tgaagccaag gtttcaaaag tttctgaaga acaattattc
     5461 tatctaatga gtcgtggtat ttctgaagaa gaagcgacag aaatgattgt tatgggattc
     5521 atcgaaccat ttacaaaaga acttccaatg gaatacgcgg tcgaaatgaa ccgtttaatc
     5581 aagttcgaaa tggaaggtag tattggatag ctttaaaccg cgttgttaag ccattcttaa
     5641 ctgtcgaaaa cggttattga taccattttg atactgaata taacaaaaag ccacattact
     5701 gtggcttttt ttgttttata actaaatcgg attgatagat aagctttgta cttatttata
     5761 tcagtccgat tttttgattg gtgtaaaaaa taatcattga tggtagataa agcgacaaca
     5821 taaatacaac atgattgtgg cattagagtg ctggtcttta ttaaattaat tgaaaactac
     5881 atcaaatatc ctttaaagat aattcgataa tagttcgatt aagtttcgtt gtataagtga
     5941 gttaaaataa gaaaactatt aataaaatta agttcactac agatgttgct aatggaccgt
     6001 aagttttaaa gacatcatta cttttataac caacaatcgc atctaaaaat tgaactaaaa
     6061 tcattgcaat ggatatagtt atcaaaaata tagcactatg aatgactaaa gaaaaaatag
     6121 ctaataaaaa taaaggtaag cttcgactaa gtgcataata tgcatttata ttatggctag
     6181 atgcacatgc ttgaattgaa taacctaaac ttacactggc actaatgatt gtaaatattg
     6241 ctaaaacaaa atacatgtta atccttcttt ctatatttga atatctgtaa acaagtactt
     6301 gtctaaagat atttaaaaga tgattagaat aaatttatga g
//
LOCUS       DAPIDM010000138         6276 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000138 DAPIDM010000000
VERSION     DAPIDM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.138"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            132..527
                     /locus_tag="RJE42_002271"
     CDS             132..527
                     /locus_tag="RJE42_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877667.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPIIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAILYILTAIATVEATKTKYRN"
     gene            723..2108
                     /gene="fumC"
                     /locus_tag="RJE42_002272"
     CDS             723..2108
                     /gene="fumC"
                     /locus_tag="RJE42_002272"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV5877668.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAQYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2563..3384)
                     /locus_tag="RJE42_002273"
     CDS             complement(2563..3384)
                     /locus_tag="RJE42_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDV5877669.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3547..4659
                     /locus_tag="RJE42_002274"
     CDS             3547..4659
                     /locus_tag="RJE42_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDV5877670.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4681..5304
                     /locus_tag="RJE42_002275"
     CDS             4681..5304
                     /locus_tag="RJE42_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5877671.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPHIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5662..6126
                     /locus_tag="RJE42_002276"
     CDS             5662..6126
                     /locus_tag="RJE42_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5877672.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
BASE COUNT         2301 a          902 c         1083 g         1990 t
ORIGIN      
        1 ttaccccaga cattatcgaa agaagtcatc tatcattaat atttttaatg aaatcattaa
       61 accgcgtttc ttaatatgcc aagattatga tatgctattg aagaatgaaa ttagaggaga
      121 tttatatatt tatgttagga aaatactgga ttcaattatt gatagcaaca gttattataa
      181 gtttaatatc aattaaagct tttccattgg caattggggc attatattta cctattattt
      241 tcaaagtaat aaaattacaa ctcaatttat caaaaggatt aatcgatgat gttaatgcgc
      301 aaacattcat taaaagtaat caatcaggta ttgtaataag tgtaatttgt tgtttattaa
      361 taacaggaat attgtattat acacttgatg ggttttatgc gagtttaaca ggagtattag
      421 gtacgcttgt tgctctaaat ccatatacga caattgttag tgctatatta tatatactta
      481 cagctattgc tacggttgaa gctactaaaa caaagtatcg taattaaact tagttcaaca
      541 ttaactaaat aatgatagat aacacactaa tcgtatgacc gtatcactgt aataaagggt
      601 gggcacgtgc gattagtgta tttttatttc aaattatgaa aacgttttaa tcataaattg
      661 ctcaattaaa ttatttaatg atatactaca gttaaataaa cagtgataag gggagaaatt
      721 gaatgtcagt aagaattgaa catgatactt ttggagaaat agaagtacct gcagataaat
      781 attggggtgc tcaaacagaa agaagtaaac gtaatttccc agttggtaaa gagcgtatgc
      841 caatcgaagt agtttatggt tttgcacaac taaagcgtgc agcagcatta gctaattttg
      901 atttaggaaa attaagcgag gcaaagaaag atgccattgt atacgcatgt gatcaaattt
      961 tatcaggtga attagatgaa cacttcccac tagttgtatg gcaaacagga agcggtacac
     1021 aaagtaatat gaatgtgaac gaagtagtaa gttatgttgc taatatgtat ttaaaagatc
     1081 atcaaagtga tgaaagcatc cacccaaatg atgatgtaaa taaatcacaa agttcgaatg
     1141 atacattccc aactgctatg cacgttgcat tatatcaaga ggttgaaaca aaattagaac
     1201 ctgcattaaa acttttaaga aatactttga aagaaaaaga agataaattt gattcaatta
     1261 ttaaaattgg tcgtacacat ttacaagatg caacgccgat caaactagga caagagatta
     1321 gtggctggcg ttatatgctt gaccgttgcg aaacaatgtt atctgaatct aagaaacaca
     1381 ttttaaatct tgccatcggt ggtacagctg ttggtacagg tattaatgca catcctgagt
     1441 ttggagacaa agtggcacag tatatttctg aaaatacggg ttatccattt gtatcttctg
     1501 aaaataaatt ccatgcatta acagcacatg atgaagtagt acaactacac ggaacattga
     1561 aggcgctagc aggagactta atgaaaattg ctaatgatgt gagatggttg gcttcagggc
     1621 cacgagctgg tttggcagaa atttctatcc ctgaaaatga accaggttca tcaattatgc
     1681 ctggtaaagt taatcctaca caatgtgaaa tgttaacaat ggttgcagtc caagtaatgg
     1741 gtaatgatac agttgttggc ttcgcaagtt cacaaggtaa ctttgaattg aatgtttata
     1801 aaccagttat tatgcataat acactacaat caatttacct tttagctgac ggtatggaaa
     1861 catttaataa caattgtgca gtgggcattg aaccaatcga agagaatatt gataattatt
     1921 taaatcaatc attaatgtta gttactgcat taaatccaca tattggttat gaaaaagcag
     1981 ctcaaattgc taagaaagcc cataaagaag gtttaacttt aaaagaatct gcaattcaaa
     2041 ctggatatgt tacagaagaa caatttgaag catggattaa accagaagat atggtagatc
     2101 ctcattaatt aaatattaat ataaaataaa gtttgggcat tacaggtaga tgtagtgttc
     2161 aagctttatt tttttgtcta tgtcaaattg aacttataag tccgatttga tatggatcca
     2221 tgaaaggaaa aacctttcat ggatcccatg ttattataga acctattttt tatatgatga
     2281 attgttgtac atatattgac caaacaatat gtgaaaaaat cttatgatgt tatgtattgt
     2341 ccatagatac acgtaacgca gttgaaaaag actacaagtc attatccaag ggtgctataa
     2401 atccaaatac attcaaaata taaaatccta agcgtcgtaa aaaacataaa aaaaccgaac
     2461 atctgaaaaa ttaaaaattt tccatctatc cgatttaagt tgatacttga tttagttaca
     2521 atgcggtatt gcatgtttaa atatcatgca aaactaatca aattagtcat ccaaagaaac
     2581 ggaaatgact tccttagtta agggatgcac aaactctatt ttgtaacttt caagttctaa
     2641 ttgtcttaac gttgaatcac cgtataaagg atcaccaata actgggtgac caatttcagc
     2701 taaatgcaca cgaatttgat gagtacgtcc agtatccaac ttgatttcaa gttggcacac
     2761 agtttctttt atcatttttg aagttaaaat gtgtgtaatt gcacgctgtc cagtaggaga
     2821 cacgcgtcgt ttattcgaat ggaatttatc tttaccaatt ggcatatcaa tagtttgtgg
     2881 ttttaaaggt aaaagtgcct tgacatttgc tttgtatatc cgcgtaatat cattgtcttc
     2941 taacatgcga tcaagaattt ttttcattaa aggattttta gcaacaatta ataatcctac
     3001 tgtttcctgg tccagtcgat gaattggttc gacataatcg ctatcaaccg tgtaaatcac
     3061 atgattcatt aaagtattgc tttcttttaa atcattaggg tgggtcttaa cacctttagg
     3121 tttcattacg attgccatat catcgtcttc gtatttaatt tgtgcataac gataacttgg
     3181 aacataatta cttttttcat cgatggttgg aacaaataca tcgtcgccag tatgcacttt
     3241 atccattaat cgcgcaggtt tatcattaat agtaatatct ttagacatat ttaaatgatg
     3301 taaatctttt ttaggtacct taagttgttg gaaaatatct cgtaaactta agtcattaaa
     3361 gttttctggt attttaaatt tcataaaatc ctcctatagt ctttaatatc atatcacaaa
     3421 tcagttctcc gaaatataac tattaagtaa tcaaggccat tttatcatga tcacgtctaa
     3481 gatttaaaaa tataataact caatgtataa tagttaatac acgtaaaaat gaattggagc
     3541 gatttgatgg aacaaaggac gcgactagct ttattaaaag agattgccga atttttaaac
     3601 gaagaaactg aaatgtatag catgacccaa ggtgcgttaa aatatttaat tgagggcagt
     3661 aatttcacga caggctggat atttttcatc aatagcgtag gtgaacatga acttgtatca
     3721 catgtggctt taccacaatc tttgacagca gaccactgtc actatattaa agatgggtct
     3781 tgttggtgtg tgaaagcatt taatcaacgt aggctaatga aagcgtcgaa tatcgtcaat
     3841 tgttctcgta ttaacttagc ctctaaagca tttcctagcc aaaatgacaa tattacacat
     3901 catgcaacgg taccacttaa atcgggtcaa gaacaattcg gcattttaaa tgtagcgtca
     3961 ccaaatactg aaatatatag tgatgaagat ttagagttgc tagagtctgt cgcatttcaa
     4021 ttagggtcag ccatcaaacg tatttattta acagaccgtg aaaaagaagc ggcaaaaatt
     4081 aatgaacgta atcgtttagc tagagattta catgattcag taaatcaaat gttattttcc
     4141 gtcaaactta cagcacatgc tgcgtatggc atgtcaaacg aatccatcgc taaacaagca
     4201 ttcaagacga ttgaggaaac gagtcaaaat gccgttaatg aaatgcgtgc attgatttgg
     4261 caacttaagc cagttggact tgagcaaggc ttaattcatg ctttgacagc atatagtaaa
     4321 ttaatgcata tacaattaaa tgtaaatgta gaaggtttaa tcgatttatc taatgaaatt
     4381 gaagaaaaca tatacagagc attacaagag tgtattaata atgttaagaa acatgctgat
     4441 acgaataaaa tggatcttac attaaaacaa atgaacgata tactatatat tgacgtgatt
     4501 gattatggtc aaggatttga gatcgataat gttcagatag cttcatcaca cggtataaat
     4561 aatataaaac aaagagttaa attattaaga ggtaaagtga cctttcattc tcagccaaca
     4621 aagggtacac aaatacaatt tacaattcct ataaaatagc ttgatttaag gagataaccc
     4681 atgaacaaag taatattagt agatgaccat tatattgtgc gacaaggatt gcgattttta
     4741 ttatccacga ttgaaaacat agaagtttta caagactttg cagatggaga aacattttta
     4801 gaatatttaa aagagcatga gcaccctgat attgtgctat tggatttagt aatgcctggc
     4861 atgaatggta ttgaaattac ggaatatatt aaggcacatt atccgcatat taaagttttg
     4921 gtattaacaa gttatgttga tgatgaacat gtaatttcag caatcaataa aggtgctgat
     4981 ggttatgaaa tgaaagacgt tgagcctcag caattaattg aaactattag acgagttatg
     5041 aacggtgaaa aaatgataca tcctaaggca caagatgtat tcgaaacagt tagccaaaaa
     5101 ccacactaca cgaataagtt gtcaaagaga gaaattgaag tgttacgtga aatggttaaa
     5161 ggtaaaacaa ataaagagat tgcagaaact ttatttgtat ctgaaaaaac aattaaaaca
     5221 catgtcagtc atatatttag taaattacaa gttagcgatc gtacacaagc agcaatttat
     5281 gcaatggaaa ataagttgat ttaaagttgc attgatgtag cttcgtcaaa aaatcgacaa
     5341 tttttaatcg atttatttag atttttttca cagcgatagc attaattata cagttttctt
     5401 tgtaaatttg atatcataat aaaagtgtaa gttttatcaa atgtagacat aatgcataaa
     5461 atttaacttc gtttactttt gtttcattat gaagtaaact taacttttgt tgacttgatc
     5521 aaaatttaat acaaatgtga aattgactct tttcatttta ttaaaaatat attaagatgt
     5581 aatctgtgtt tcaagaaatg tgtattattg caatttcttg tgtccggata aaattcccca
     5641 aacagaaagg tagataacag aatggataga cagagtttta cagatttaat tcaaacaaaa
     5701 tttaaaatgg ttcgtataga ggctggttat acgcaagata ctatggcgca aacaattgga
     5761 ctttctaaaa agactttagt acaaatcgaa aaagagagag tattaccaaa ctggacaact
     5821 tgtatttcaa tttgtgcatt attcagagac tctgaagtat taaacagtac atttggctgt
     5881 gatccattag aaatcgttca aacaatttca agaaatcact gtgcatatcc aaaccatgca
     5941 ccaacaagtg atatttattg gaataacatt gagactcgta atgggtacat tttacaaagt
     6001 aacaaagtaa gcaatattta tcgtgtatta aatccagata accaaccaat tttcggtact
     6061 tctaaaatga gagaagctga aacatacttt aacagaaatg ctaaagaaga attagtacat
     6121 atctaggcct atatactttt aataaataat aaaagcgatg taacacacct gtttcgagag
     6181 gtgaggcatc gctttttgta tgtaaactaa taataaattg ggtagaaagt gaaatgatta
     6241 atgaattaaa aaataaaaat agtggtataa tgaata
//
LOCUS       DAPIDM010000139         6271 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000139 DAPIDM010000000
VERSION     DAPIDM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.139"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..2626)
                     /gene="alaS"
                     /locus_tag="RJE42_002277"
     CDS             complement(<1..2626)
                     /gene="alaS"
                     /locus_tag="RJE42_002277"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV5877673.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSEKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKN"
     gene            complement(2969..5446)
                     /locus_tag="RJE42_002278"
     CDS             complement(2969..5446)
                     /locus_tag="RJE42_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDV5877674.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEDMLENTATNDTVASQTTIDDQVEAPMSSNDSEEVTSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(5448..6116)
                     /locus_tag="RJE42_002279"
     CDS             complement(5448..6116)
                     /locus_tag="RJE42_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV5877675.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDTEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
BASE COUNT         1877 a         1227 c          865 g         2302 t
ORIGIN      
        1 gatttttaat gtaatcttta ataaagctta atgattttga gatattttca ggttgtgtac
       61 cgccaccttg agccatatct ggacgaccgc cacctttacc accaacgatt ggtgccattt
      121 gtttgataag atcaccggct ttaacattat ttgttaaaga tttagggaca gttgcaacca
      181 tcgatacttt atcatcaaca ttacttgcaa gaatgataat tgtatcttgt agtttagatt
      241 taaaatcgtc cattgtcgag cgaattgctt tcgcatttgg tacatccact tcagtaacca
      301 atactttata gccattgatt tcttcaactt gatcttcaat attacccatt ttaagtgatg
      361 tgatttcttt gtcacgttgc tctaattgtt ttaataatgc tttttcttca tcttgcaatt
      421 gtgttaactt atcgactact tgatcatcag atttcacttt cagctgtgat ttcatcgtat
      481 taaatttctc ttgaatatct tctaaatata agaaagctgc tttacctgtt aatgcttcaa
      541 tacgacgcac accagctcct gtacctgact cacttactat tttgaataag ccaatttcag
      601 aagtattgcg gacatgaata ccaccacata attcaattga aaatggtgcc atatttacta
      661 cacgcacaac atcaccatat ttttcaccga ataatgccat tgcgcccatt tctttagctg
      721 aagcaatatc catttcttga atgttaacgt caataccttt ccaaatttct tcatttacta
      781 agcgttcaac ttgatcaatt tcatcattag tcattggacc aaaatgagag aaatcaaaac
      841 gtaaacgatc tgcttctact agtgaaccag cttggttaac atgatcaccc agtactgatt
      901 tcaacgctgc atgtaataaa tgtgttgcac tatggttctt ttgaatgtca cgtcgatcat
      961 tttggttcac ttcagctgac actgtagcgc caacatttac ttggccaaat tgtactactc
     1021 ctttatgcaa gttttgacca tttggtgctt tggttacttc actaacagca atttcaaaat
     1081 tgtcattata tacaatacct gtatccgcaa cttgtccacc actgactgca taaaatggtg
     1141 tttccgttaa catgaagtat actgtttcac ccgcttcaac ttgtgaaact tcttcaccat
     1201 tatatatcaa gtgtgttagt gtcgtttgag ctgtcgcagt atcataacca acaaaagtac
     1261 ttgcagatgt aatatttttc aatacttcac tttgaacttg cattgattga gaattttgac
     1321 gtgcttgacg agcgcgatca cgttgttgtt gcatttctga ctcgaatgtt gtcatatcaa
     1381 ctttcaatcc tgcttgaact gctatttctt cagttaattc aattgggaac ccgtacgtat
     1441 catacaattt aaatgcatct ttcccattaa tttcatttgt tgtcgcttta gcttttttaa
     1501 ttaattcatt taaaatcgct aaaccatctt ctaatgtttc atggaatcgt tcttcttcag
     1561 actttataac acgcttaatg aaatctgctt tttccttaac atttggataa tatggttcca
     1621 taatgtctgc aacaatatca acaagtttgt acataaatgg ctcattgatt cctaacgttt
     1681 gactaaaacg aacggcacga cgtaacaatc gacgtaatac atatcctcta ccttcattgg
     1741 caggtaatgc accatcagaa attgcaaatg caatcgtacg aatgtggtca gcaattactt
     1801 taaatgccac atcttgttcg ttgtttacta aatattgttt acctgatact ttttcgattt
     1861 cattcattat aggcataaat aaatctgttt catagttagt acgtacattt tgagaaactg
     1921 aggccatacg ctcaagcccc atgccggtat caatattttt attaggtaat ggtgtgtaac
     1981 tatgatcttt attatgattg aattcactaa atactaagtt ccatacttca agatagcgtt
     2041 cattttctcc acctggatac atttcttccg caggatcatc ttgtccatat gcttctccac
     2101 gatcatagaa gatttcagtg ttcggtcctg atgggccttc accaatatcc cagaagttac
     2161 cttcaatgcg aataatacga ctttcttcaa gcccaatatc tttatgccaa atgttgtatg
     2221 cttccatatc ttccggatga atcgtaacgt acaatttatc tggctccata cccatccatt
     2281 tttcactcgt taaaaattcc caagcaaatt caatcgcttc ttgtttaaag taatcaccaa
     2341 ttgagaagtt acctaacatt tcaaagaatg tatggtgacg cgctgtgaaa ccaacatttt
     2401 caatatcatt tgtacgaata gctttttgag agtttacaat tcttggcttt ttaggtgttt
     2461 cacgtccatc aaaatatttc tttaatgttg ctacacctga attaatccat aataatgtat
     2521 catcatcaat tggcactaat ggtgcagaag gttcaaccat atgtcctttt tcaacaaaga
     2581 aatctagata tttttgtcta atttcactcg cttttaactt tttcatcatt tacacatcct
     2641 atttactgtt tttaaattac cattccataa aaattgatga cacagatagt cgatttgaaa
     2701 aactagtata aatcaatatc actttttatt attaaaaaat aaaaaacgcc catcctcaaa
     2761 agggacgaac gttatcgcgg taccacccta gttataaatg caattcaata catttatcac
     2821 tttaaatcga ctatacagtt gtgcataaag tagcgttcac taatgtttgt tgtacttttc
     2881 accaaccagt acatctctga taaacaaatc attaactact catctttata cgaatttaat
     2941 tctattttag ttacatttac gcttgttgtc aacgttctat aaagtcatat ggcgtgattt
     3001 ctcccatatt aatcattggg tcaattttaa acattgtagc ttccgttaat acatttgtat
     3061 ctgtttttgt tgaatcagac gtaacttctt cactatcatt cgatgacatt ggcgcttcta
     3121 cttgatcatc tattgtcgtt tgtgaagcta ctgtatcatt agttgctgtg ttttccagca
     3181 tgtcttcatc ttctgaatta aaataatttt tcaacaatgt acataattgt gttaaacgcg
     3241 cttgaccatt tgttttcaat ccaatatcaa atgcttctgg atcaccaagt aaaactaaac
     3301 tcgttttcgc tctagttaaa ccagtatata atatcggtct ttgtaacatt ctaaaatact
     3361 gtttaacaat aggcatgata acaataggaa attctgaacc ttgcgattta tggattgatg
     3421 tacaatatgc atgtgttaat tccatcatat cttgtttcgt aaatgtaatt tcattacctt
     3481 caaaatccac aacaagcaca tctttattaa gggcattttc tttcgcccaa aaaataccaa
     3541 caataactcc tatgtcacca ttaaatatgt tatcatttgg cctattaaca agttgtaata
     3601 ctttgtcacc ttttctaaag actacatcac caaactcaat ttcacgtgtg tctttctttt
     3661 tagggtttaa aatatcttgt aaaacttgat ttaaacgttt aataccagca tttcctttat
     3721 acattggtgc aagcacttga atatcagcca tagtataccc tttattaaca gcactagtaa
     3781 ctaccttctc aacaactgtt ggtatttggt ttgcctgaca gttaataaaa cttctatcat
     3841 gaaaacgctg tgtaatatca attttctgac ccaacttcat tcgatgtgct aattctataa
     3901 tgcttgaacc atcttgttga cgatatactt cagtcagatt aactcgtggt atagctttcg
     3961 attcaattaa atctttaaat acttgaccag gacctacaga aggcaattgg tcctcatcac
     4021 ctacaaatat caattgtgca tctaaaggaa ctgcacttaa aaattggtgg aacaaccaag
     4081 tatctaccat agacatctca tcaatgatta taagtcgtgc gtttatttca ttttctaata
     4141 tatcctctgg ctttgtgtct tgattccaac ctattaaacg atgaatcgtc atagcttcta
     4201 atccagttga ctcttgtagt ctcttggacg ctcttcctgt tggcgctgct aatacaactg
     4261 gataatcatc attgacataa tcatcataat ctaatgataa gccatgaatc tcagcatata
     4321 attctacaat gcctttaatt actgtcgtct ttcctgttcc cggtccaccg gttaatagca
     4381 tcaccttaga attgatagcc gtttgcaaag cttctttttg tgaagctgca tagttcactt
     4441 gattcgcatc ttctatttca ccaatatgca tttgtaaatc tgactgttca atttctgtaa
     4501 gtttatttgt atgcgtcttt attctgaata agttttgaac acttttgatt tcagaataat
     4561 acaaacttgg aattgcaact tgttcattgt caataattag tcgtttttcc tcatttaagt
     4621 attgcaacat ttcgtctaat ttttcaggtt cgatgacctc ttcatcttga taatttaata
     4681 catcaaccgt taaatctata acaacattga taggcaaata tgtatgtccc tgtttaatac
     4741 attcttcttc taacgtatag agcaacgcag cttttaatcg ttcattatcg ttataagcga
     4801 taccaatatt tctagcaagt tgatctgctt tattaaaacc aataccttta atatcataaa
     4861 tcaattgata tggatttcga tctaaaatag tcagtgtatc accgagataa aactgataaa
     4921 ttgccattga aagtttagga ccaaacccta aatcatgtaa acgaatcatt attttttcag
     4981 attcttgatt tgctgaaatt tgttctgcaa tttgtttctg tttcttttta gataatcccg
     5041 aaactttttc tagaactgaa tggtcatcta atatatcatt tatcgcattg tcacctaatg
     5101 tattaacaat attttgagct gtctttttac ctacaccttt aaacaaatca cttgataaat
     5161 aacttataat tgcttctttc gtttgtggca tttctttttc aaaagtttct gcttttaatt
     5221 gtttaccata acgtggatga tcaacaacct gccctttaaa tgtgtagaca tcgccttcaa
     5281 caatattcgg aagaaaccct acaacagttg gcattgtatc aaagtcttca tttgtttcaa
     5341 tagtatctac tttaagcact gtataaaaat tatcactgtt ttgaaacaat atcgcttcaa
     5401 cagtaccttt gatcattgaa taatcaaata gtgtagggtc tgacatgtta ctcctcctct
     5461 ttcattttag tgaacgtttt cagcgcatgc tgacttaata agtgtttagg gtcgatagtc
     5521 acagcttctt taaaatgagt tatggcttca tcaatatctt catttttcat aaataacgct
     5581 aaacctaaat tgtatcttgc atcaacatga tttttatcaa tcgtcaatac atgtttaagt
     5641 tgagttatgg cttcattaaa catttctaat tgacataata caagaccata ttgaaattga
     5701 acttctgtat ctttgtcttt atctagttcc gcagcagtca ttaaatacgg caatgccagc
     5761 ttaaatgatt ctaactgatt aaacgccata ccgatcatat aattacaatc aacttgttca
     5821 atctctgttt gtaatgcttg ttgatataat ttaatagctt cttgataacg ttgctgatta
     5881 taatatacat ttgctagatt ataaaatacg acgccattct tcggatctat tgttaaagct
     5941 ttttggaaaa aacgctctgc cttttcaatc tcattcgcat cagcaagtac gataccagca
     6001 ttaatataat tttcaataat tgtaggatct tcttcgatat ttccgaacaa tgcttgtaac
     6061 gcttcttcta tttttccatt ttgtatgtat tgataaattg tttgttgatc tatcatttac
     6121 gaacctcatt tctcatcaat tataacatct tgataaattg tatgtctcga atcacttaac
     6181 aacgaattaa atataatcta atatcatctt cattcatgaa aaagcgggaa tggaatagaa
     6241 atgctaaaga accattaacg ttttattatg t
//
LOCUS       DAPIDM010000140         6233 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000140 DAPIDM010000000
VERSION     DAPIDM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.140"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..1055)
                     /locus_tag="RJE42_002280"
     CDS             complement(<1..1055)
                     /locus_tag="RJE42_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDV5877676.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRFQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYT"
     gene            1330..1953
                     /gene="gmk"
                     /locus_tag="RJE42_002281"
     CDS             1330..1953
                     /gene="gmk"
                     /locus_tag="RJE42_002281"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV5877677.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            1953..2171
                     /locus_tag="RJE42_002282"
     CDS             1953..2171
                     /locus_tag="RJE42_002282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV5877678.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            2387..3586
                     /gene="coaBC"
                     /locus_tag="RJE42_002283"
     CDS             2387..3586
                     /gene="coaBC"
                     /locus_tag="RJE42_002283"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV5877679.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETQKFIAPAMNVHMYENKRTQQNINTLKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            3586..5994
                     /gene="priA"
                     /locus_tag="RJE42_002284"
     CDS             3586..5994
                     /gene="priA"
                     /locus_tag="RJE42_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV5877680.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKED
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQFKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSDLSNGERYDEWQKIRNGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2214 a          901 c         1169 g         1949 t
ORIGIN      
        1 gtataataat tcaatgctgt cacttcttta tcaccttgct taattcgata tatattagca
       61 gtgatcaatt caccatataa ctgttcagta tctttatttt tagactgctc atattcttca
      121 atcaacttcg ctaatttatt ttgatatttg tgcaactgct gttgaacaaa tcgaactaaa
      181 tcattcgcac gttgtttaac gcgttcccgt tcgccacgcg catcataaaa acgatcaagt
      241 aaatcattta atgaatcgta cgtaactgta tcatcattaa attgatttaa ctttataaaa
      301 tagaaatcct ctttacctgt ttcatgattt ttatgaaaaa taggagtagg tggtaacttg
      361 gtttctgcca ttacttcatc gaatgcttct ggtaaagttg atgaagtcat aaattgacga
      421 cgattaacga tttcattcgt aattaaaggg ctaaatcctt caaactgatt caataattgt
      481 ttagcaatat tacctgcgtt aaaatcgata tattttaaca cctctgcacc tgtaatatca
      541 tacggattca ttttgtgctg agtaggtggt gcttcataat taaaacctgg cattactgta
      601 cgatagtgat tcgtatttgg tgttaagtgt ttaaatcctt caattatttt gcgattttca
      661 tctactaaaa ttaagttact atgtttaccc ataatctcaa ggatgacagt gcggtaaata
      721 gtatcgccaa tttcatcttt actctttata tcgatttcaa tacgacgatc attaccaatt
      781 tgcttaatcg attcgataat accaccttct aagtgttttc taaaaacacg cgcaaacatg
      841 ggtggattaa atggattatc atatttttta gtagtcaatt ggaatcttga aaagtttgga
      901 tggattgaca ataacaattg atggttttgt ctattttgac gtacaaccat tagtatcgtg
      961 tcattatcag gttgattgat tttgtgaacg cgtcctgttg ttaaaaattg tagagactca
     1021 accattttct ttgtaaataa gccatcataa gccataattt atcttccacc ttcttcatat
     1081 cattcgtttc attttaacat gattgtaact tttcggtata gttattgaat catacattat
     1141 gccacacatt taacattatc taaaagttcg caattcaatt tatttaatta aaattaatcg
     1201 tgcaatataa cgatattgtt agacctaata gaaattatgg catgcaattt caaatatgct
     1261 atacaatata aagaacaatg tgatatcata tttaaataat agaagattag cttagagagg
     1321 tcgtaaggca tggataatga aaaaggattg ttaatcgttt tatcaggacc atctggagta
     1381 ggtaaaggta ctgttagaaa acgaatattt gaagatccaa gtacatcata taagtattct
     1441 atttcaatga caacacgtca aatgcgtgaa ggtgaagttg atggcgtaga ttactttttt
     1501 aaaactaggg atgcgtttga agctttaatt aaagatgacc aatttataga atatgctgaa
     1561 tatgtaggca actattatgg tacaccagtt caatatgtta aagatacaat ggacgaaggt
     1621 catgatgtat ttttagaaat tgaagtagaa ggtgcaaagc aagttagaaa gaaatttcca
     1681 gatgcgttat ttattttctt agcacctcca agtttagatc acttgagaga gcgattagta
     1741 ggtagaggaa cagaatctga tgagaaaata caaagtcgta ttaacgaagc acgtaaagaa
     1801 gtcgaaatga tgaatttata cgattacgtt gtagttaatg atgaagtaga acttgcgaag
     1861 aatagaattc aatgtattgt agaagctgag cacttaaaaa gagagcgcgt agaagctaag
     1921 tatagaaaaa tgattttgga ggctaaaaaa taatgttaaa tccaccatta aaccaattaa
     1981 cgtcacaaat taaatcaaag tatttaattg caacaactgc agcgaaaaga gcgcgtgaaa
     2041 ttgatgaaca acctgaaact gaattattaa gtgaatatca ttcatttaaa ccagttggta
     2101 gagcgttaga agaaattgct gacggtaaaa ttcgccctgt tatttcaagc gattattatg
     2161 gtaaagaata gtttcgacca ttaaaaatat gtgaagcaag acgtacatca aatacgtcta
     2221 ggcatatcca aatcaaacgc acctatgtag gtgtgttttt ttagtttatc caatgattga
     2281 tgttataata atactaaatt tgtatctata aaaaagtaat gagcatttgt gcgcatatga
     2341 tgatgtaaag cgtaaatgtc atagtgatat ttaagggaga ttgaatatga agaaaatatt
     2401 gttagccgtt acaggtggca ttgcggcata taaagcgatt gatttgacaa gtaagttaac
     2461 acaatctggg tatgaagttc gcgttatgtt aacgaatcac gcgcaaaaat ttgtgacacc
     2521 attagcattt caagcaataa gtcgaaatgc tgtttataca gatactttta tagaagaaaa
     2581 tccttcagaa atacagcata ttgcattagg tgattgggca gatgcaatca ttgttgcacc
     2641 tgcaacggca aatacaattg caaaattgag tgtaggtatt gctgatgatt tggtgacatc
     2701 aacgttgcta gcaacagaga cacagaaatt tattgcgcct gctatgaatg tgcatatgta
     2761 tgaaaataaa cgcacgcagc aaaatattaa tactttaaaa gaagatgggt atcattttat
     2821 cgaaccagga agcggatttc tagcatgtgg ttatgttgct aaaggacgta tggaagaacc
     2881 gcttcaaatc gtttctgtta ttgatgctca ttttcaaaat agtaatcgtt tagctaatag
     2941 ttcatttcaa gataaacgcg cattggttac agctggacca actattgaag ttatcgatcc
     3001 agtcagattt gtatccaatc gttcttctgg aaaaatgggt tatgcaatag ctgaagcatt
     3061 gcgaaatcga ggagctatcg tgacgttagt tgctggtcct acaacactag aagatccaaa
     3121 agatattgaa gttattcatg ttcaaagtgc tgaagaaatg tttgaacaag tgacaagccg
     3181 atttgacgaa caagatattg ttgtaaaagc agcagccgta tctgactata caccggttga
     3241 tgtattagaa cataagatga aaaagcagga tggtgatttg tcagtatctt ttaaacgtac
     3301 taaagacatt cttaaatatt taggtgaaca taaaacatca cagtatttaa taggctttgc
     3361 agcagagact gaagatattg aaaattatgc acaacaaaaa ttacgcaaga aaaatgcaga
     3421 tgtgattatt tcaaataatg ttggggatat gtctatcgga tttagttctg atgataatga
     3481 attgacaatg cattttaaaa ataatgaaaa agtaaatatc aagaaaggaa aaaaagtagt
     3541 attagctgca caaattttag atgaactaga aactaggtgg caataatgat agcgaaagtc
     3601 atagtcgatg tcgcgtcgaa gagcgttgac tataaatttg attatataat tcccgaacaa
     3661 ctcgaatctg tcatccaacc tggtgtgcgt gtaattgtac cttttggacc aagaacgatt
     3721 caaggttatg taatggaagt aacagcagaa cctgatgcac aacttgacgt ttcgaagtta
     3781 aaaaaaatca tagaagtgaa agatatacaa ccagaattaa catcagaatt aatagcttta
     3841 agtgagtgga tgggttcaac tcatgtcatt aaacgtattt ctatgttaga agtgatgctt
     3901 ccgagtgcta ttaaagcgaa gtataaaaaa gcatttaaga tgaaagatga catagaggta
     3961 ccttcagctt tattacaaaa atttgataag catggttact attattataa agatgcgcaa
     4021 aaaaataatg atattcaatt acttatgaag ttgttaaaag aggatatcgt tgaagaaaaa
     4081 acgattctca cacaaaatat aactaaaaaa actaagcgtg ctgttcgtgt cattgaaggg
     4141 tatcatcctg atgaagtatt agctaaattg gagaaagtta ttaaacaata cgatttgtat
     4201 gcttacttgt ctgaagaaca acataaaaca atatttttaa ctgatattga ggatatgggc
     4261 ttttcaaaat ccagtttaga tggacttatc aaaaaaggtt atgttgaaaa atatgacgcg
     4321 gttgttgaaa gagacccatt taaagatcgt gttttcgaac aagaatcaaa acagcaatta
     4381 acagaagacc aatttaaagc atatgaagcg attaaagcta aaattgtaag ccaagagcaa
     4441 gaaacatttt tacttcatgg tgtgacggga tcaggtaaaa cagaagtata tttacaaacg
     4501 atagaagatg ttttaagcca aggaaaacag gcgatgatgt tagttcctga aatcgctcta
     4561 acaccgcaaa tggttttacg cttcaaacgt cgatttggtg atgacgttgc tgtattacat
     4621 tctgacttat ctaatgggga acgttatgat gagtggcaaa aaataaggaa tggtcgtgcg
     4681 agagtaagtg ttggtgcaag gtcaagtgtg ttcgcacctt tcaaaaattt aggattaatc
     4741 attattgatg aagaacatga atctacatat aaacaagaag attatccgag atatcacgct
     4801 agagaaattg cccaatggcg aagtgaatat catcactgcc cagtcatttt aggaagtgca
     4861 acaccatgtc ttgaaagtta tgcacgagct gaaaaaggtg tttatcattt gctatcatta
     4921 ccaaacagag tgaaccaaca agctttacct gaaattgata tcgtagacat gcgtgaagaa
     4981 ttgagtgaag gtaatcggtc aatgttttca aaagatttac gtgaagccat acaattaaga
     5041 ttagatcgac aggaacaagt tgttttattt ttaaatcgac gtggttatgc atcatttatg
     5101 ttatgtcggg attgtggata tgtaccgcaa tgcccaaact gtgatatttc attaacgtat
     5161 cataaaacga cagacttatt aaaatgtcac tattgtggtt accaagagac gccaccgaat
     5221 caatgtccaa attgtgagag tgaacacatt cgacaagtag gtactggtac tcagaaagtt
     5281 gaagaactat tgcaacaaga atttgaagat gcgcgcataa ttaggatgga tgtagataca
     5341 acctcaaaga aaggtgcaca tgaaaagtta ttgactgaat tcgaaaaagg taacggtgac
     5401 attttactag gtactcagat gattgcgaaa ggattagatt atccaaatat tactttagtt
     5461 ggtgtgctga atgcagatac aatgttaaat ttacctgatt ttcgggcgag cgaacgtact
     5521 tatcaactat taacgcaagt ggctggtaga gctggtcgtc atgaaaaggc aggtcaagtc
     5581 atcattcaaa cgtataatcc agatcattat tcaatattgg atgttcaaaa aaatgattat
     5641 ttaacatttt atcgtcagga aatggaatat cgtaaattag gaaagtatcc accgtattat
     5701 tatttgatta atttcacaat ctcacataaa gaaatgaaga aggttatgga agcatcgcaa
     5761 catgttcata aaattttatt acagcattta acagaaaaag cgcttgtact aggtccatct
     5821 ccggcagcac ttgcgagaat caacaatgaa tttagattcc aaattttagt gaaatataaa
     5881 agtgaacctg gattattaca agccattcaa tttttagatg actattacca tgaaaaattt
     5941 ataaaagaaa aattagcatt gaagattgat attgatccac agatgatgat gtaatttttt
     6001 gactaaaaca ctttaatatc aatgtttcct aagagtatga aaggtcttgt ttttacttta
     6061 agtacagaaa aagggcagaa tatacaactg ttaaccattt aaattagcag tttatattct
     6121 gtctttttat atggctttat aacttacgtg attttgattt gattagggat ttattagtat
     6181 tttcatttac gaattctgat tttaatgtaa gtgccatact taatgataaa caa
//
LOCUS       DAPIDM010000141         6227 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000141 DAPIDM010000000
VERSION     DAPIDM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.141"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..485
                     /locus_tag="RJE42_002285"
     CDS             <1..485
                     /locus_tag="RJE42_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDV5877681.1"
                     /translation="ESFLAIQSSMFANFNASNSNLVTFSAVLLVIILVITIFLLPYLD
                     VLLLGRAEAINLGISYEKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKT
                     YEHKYILIATICLSWISLFSAQWIVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ
                     "
     gene            482..1243
                     /locus_tag="RJE42_002286"
     CDS             482..1243
                     /locus_tag="RJE42_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877682.1"
                     /translation="MIQVGNLTKTINNQMILDDISIEIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHEIVNDALDLLQLQDIRKRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVAMKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIRNQRICLYFKD"
     gene            1362..2390
                     /locus_tag="RJE42_002287"
     CDS             1362..2390
                     /locus_tag="RJE42_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV5877683.1"
                     /translation="MKKTVLYLVVAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLISSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKKFNKS
                     VMYLLVNEGELSTYGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYINKENPDV
                     ILAMDRGQAVSGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     DELEKVVK"
     gene            complement(2707..3021)
                     /locus_tag="RJE42_002288"
     CDS             complement(2707..3021)
                     /locus_tag="RJE42_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877684.1"
                     /translation="MPIVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(3039..3962)
                     /locus_tag="RJE42_002289"
     CDS             complement(3039..3962)
                     /locus_tag="RJE42_002289"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV5877685.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKFTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(4089..4607)
                     /locus_tag="RJE42_002290"
     CDS             complement(4089..4607)
                     /locus_tag="RJE42_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDV5877686.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDRLLSTNDEIALQFNTKKHQILQHSFTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            4727..5605
                     /locus_tag="RJE42_002291"
     CDS             4727..5605
                     /locus_tag="RJE42_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDV5877687.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            5759..6079
                     /gene="ytxJ"
                     /locus_tag="RJE42_002292"
     CDS             5759..6079
                     /gene="ytxJ"
                     /locus_tag="RJE42_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDV5877688.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
BASE COUNT         2318 a          849 c         1058 g         2002 t
ORIGIN      
        1 ctgagtcatt tttagcaata caaagtagta tgtttgctaa ttttaatgct tctaattcga
       61 atttagttac tttttcagca gtattactag taatcatatt agtcattaca atttttctat
      121 tgccatattt agatgtattg cttttaggtc gtgctgaagc aattaatctg gggatatcgt
      181 atgaaaaatt aacgcggatt ctacttgtaa tagtttctgt cttagtctct gtgtcaactg
      241 cattagtagg accaattaca tttttaggct tattgactgt aaatctagcg catgaactaa
      301 tgaagacgta tgaacataaa tatatattaa tcgcgacaat ttgcttgagt tggattagtt
      361 tatttagtgc gcaatggata gttgaaaatg tgtttgaggc tacgacagaa atgagtatac
      421 ttattgattt aattggtgga agttatttca tttatctatt agttagaagg agaaatgcgc
      481 aatgattcaa gttgggaatt taactaaaac tataaataat caaatgatat tggacgatat
      541 tagcatagaa attgaaaaag gtaagttgac ttctttaatt ggacctaatg gagcagggaa
      601 gagtacgtta ctttcagcga tatgtcgctt aattcggttt gatgaagggg aagtaaaaat
      661 agatggacag cttatgtctg attataaaaa caatgatttg tcgaaaaaga tatcgatttt
      721 aaagcaaaca aaccatacag aaatgaacat tactgtagaa caattagtta atttcggacg
      781 atttccatat tctaaaggac gtttaacgaa agaagaccat gaaattgtta atgatgcttt
      841 agatttgttg caattacaag atattagaaa gcgtaatata aaatcattgt ctggtggaca
      901 acgtcaacgt gcctatatag cgatgaccat tgcacaagat actgaatata ttcttttaga
      961 tgaaccgtta aataatttag atatgaagca tgctgttcaa attatgcaaa cgttaaaaat
     1021 gttagcgcat aaaatgaata aagcgattgt cattgtgtta catgatatta actttgcgtc
     1081 ctgttattca gatcagattg tagcaatgaa aaacggacaa ctagttaaat cagatttgaa
     1141 agataatgtc attcaaagta gtgttttaag tgatttatat gacatggata ttcaaattga
     1201 acatataaga aatcaaagga tttgtttata ttttaaggat tgataatttg gagacacttt
     1261 aaaggggtga tgcaccaatt aaagaagggt taaacgtaaa gcatttattt atatttcaca
     1321 tcaagcacac agattaagcc aaaagaggag aatattatat tatgaagaaa acagtcttat
     1381 atttagtagt agcagtaatg tttttattag cggcatgcgg taacaattct gataaagaac
     1441 aatcaaaatc agaaactaaa ggttctaaag atacagtaaa aattgaaaac aactataaaa
     1501 tgcgtggcga gaaaaaagat ggtagcgatg ctaaaaaagt taaagaaact gttgaagtac
     1561 caaagaaccc taaaaatgca gttgtattag attatggtgc attagatgta atgaaagaaa
     1621 tgggcttatc agacaaagta aaagcattac ctaaagggga aggcggcaag tcattaccga
     1681 atttcttaga gtcatttaaa gatgacaagt atacaaatgt tggtaacttg aaagaagtta
     1741 atttcgataa aattgcagca acaaaaccgg aagtaatctt tatctctggc cgtacagcaa
     1801 atcaaaagaa tttagatgaa tttaaaaaag ctgcaccaaa agcaaaaatt gtttacgttg
     1861 gtgcagatga aaagaattta attagttcaa tgaaacaaaa cactgaaaat atcggtaaaa
     1921 tttacgataa agaagataaa gctaaagaat taaataaaga tttagataac aaaattgctt
     1981 caatgaaaga taaaactaag aagttcaaca aatcagttat gtatttatta gttaatgaag
     2041 gtgaattatc tacttatgga cctaaaggtc gtttcggtgg attagtttac gatacattag
     2101 gtttcaacgc ggttgataaa aaagtaagta acagtaacca cggacaaaat gtctctaatg
     2161 aatacattaa taaagaaaat ccagatgtta ttttagcaat ggatagagga caggctgtaa
     2221 gtggtaaatc aactgcgaaa caagcattaa ataatcctgt attaaaaaat gttaaagcaa
     2281 ttaaagaaga caaagtttat aatttagacc ctaaattatg gtattttgca gctggatcaa
     2341 ctacaactac aattaaacaa attgatgaac ttgaaaaagt tgtaaaataa ttttaaaaga
     2401 ggggaacaat ggttaaaggt cttaatcatt gttcccctct ttactttaaa aaaggaaatc
     2461 tgggacgtca atcaatgtcc tagactctaa aatgttctgt tgtttgtcgt tggttgaatg
     2521 aacatgtact tgtaacaagt tcatttcaat accagtgggc tccaaacata gagaaatttg
     2581 atttccaatt tctactgaca atgcaagttg acggagccca aacatagaga atatcaaaaa
     2641 gaaattctac agaagtggtg ctttatcatg tctatcccac tccctataat tttttggcta
     2701 tgttgtttaa atttcgaaat aaatatgata gtgatatttg cagcgattgt taaatcgaga
     2761 ttggcaatta ggacaacgct caaccatcat atattcattg attgttaatt catgtttgca
     2821 tacaccgcat aagattgctt tttcgttaaa tgaaggctca gaccaacgct taatggcgtg
     2881 cttttcaaac tcattatggc acttatagca tggatagtat ttattacaac atttaaattt
     2941 aattgcaata atatcttctt cggtaaaata atggcgacag cgtgtttcag tatcgattaa
     3001 tgaaccataa actattggca tagacaaagc tccttaactt acgattcctt tggatgttca
     3061 ccaataatgc gaacttcacg atttaattca atgccaaatt tttctttgac ggtcttttgt
     3121 acgtaatgaa taagattttc ataatctgtt gcagttccat tgtctacatt taccataaaa
     3181 ccagcgtgtt tagttgaaac ttcaacaccg ccaatacggt gaccttgcaa attagaatct
     3241 tgtatcaatt tacctgcaaa atgaccaggc ggtctctgga atacactacc acatgaagga
     3301 tactctaaag gttgtttaga ttctctacgt tctgttaaat catccatttt agcttgtatt
     3361 tcagtcattt taccaggagc taaagtaaat gcagcttcta atacaactaa gtgttctttt
     3421 tgaataatgc tattacgata atctaattct aattcttttg ttgtaaattt aattaacgag
     3481 ccttgttcgt ttacgcaaag cgcatagtct atacaatctt taacttcgcc accataagcg
     3541 ccagcattca tatacactgc accaccaatt gaacctggaa taccacatgc aaattcaagg
     3601 ccagtaagtg cataatcacg agcaacacgt gagacatcaa taattgcagc gccgctaccg
     3661 gctataattg catcatcaga tacttcgata tgatctagtg ataataaact aattacaatg
     3721 ccacgaatac caccttcacg gataataata tttgagccat tgcctaaata tgtaacagga
     3781 atctcatttt gataggcata tttaacaact gcttgtactt cttcattttt agtaggggta
     3841 atataaaagt cggcattacc acctgtctta gtataagtgt atcgttttaa aggttcatca
     3901 actttaattt tttcatttgg gataagttgt tgtaaagctt gatagatgtc tttatttatc
     3961 acttctcagt acatcctttc tcatgtcttt aatatcatat agtattatac caattttaaa
     4021 attcatttgc gaaaattgaa aagaaagtat tagaattagt ataattataa aatacggcac
     4081 tattgtcgtt ataagtattt tttacatagt ttttcaaagt attgttgctt ttgcatctca
     4141 tattgtctaa ttgtaaagct atgttgcaat atttgatgct ttttagtatt gaattgtaaa
     4201 gcgatctcat cattagtcga taagagacga tcaagtgcaa gataagattc aaatgtttgg
     4261 gtattcattt gaatgatatg tagacgcacc tgttgtttta gttcatgaaa attgttaaac
     4321 ttcgccatca taaccttttt attatattta tgatgcaaac gataaaaccc tacataattt
     4381 aagcgttttt catctaagga tgtaatatca tgcaaatttt ctacacctac taaaatatct
     4441 aaaattggct ctgttgaata tttaaaatga tgcgtaccgc caatatgttt tgtatatttt
     4501 actgggctgt ctaagaggtt gaataataat gattcaattt cagtgtattg tgattgaaaa
     4561 caattagtta aatcactatt aatgaatggt tgaacatttg aatacatgat aaactccttt
     4621 gatattgaaa attaatttaa tcacgataaa gtctggaata ctataacata attcattttc
     4681 ataataaaca tgtttttgta taatgaatct gttaaggagt gcaatcatga aaaaaattgt
     4741 tattatcgct gttttagcga ttttatttgt agtaataagt gcttgtggta ataaagaaaa
     4801 agaggcacaa catcaattta ctaagcaatt taaagatgtt gagcaaaaac aaaaagaatt
     4861 acaacatgtc atggataata tacatttgaa agaaattgat catctaagta aaactgatac
     4921 aactgataaa aatagtaaag aatttaaggc actacaggaa gatgttaaaa accatctcat
     4981 acctaaattt gaagcatatt ataagtcagc aaaaaatttg cctgatgata caatgaaagt
     5041 taagaaatta aaaaaagaat atatgacgct tgcaaatgag aagaaggatg caatatatca
     5101 attaaaaaaa ttcataggtt tatgtaatca atctatcaag tataacgaag acattttaga
     5161 ttatacgaaa caatttgaaa aaaatagata caaagttgaa tcagaaatta aattagctga
     5221 taataaaagt gaagcaacta atcttacgac aaaattagaa cataataata aagcgttaag
     5281 agatactgcg aaaaagaacc tagatgatag taaagaaaat gaagtaaaag gcgcgattaa
     5341 aaatcacatt atgccaatga ttgaaaaaca aattaccgat attaaccaaa ctaatattag
     5401 tgataagcat gttaataatg caaggaaaaa cgcaatagaa atgtattaca gtctgcagaa
     5461 ctattataat acacgtattg aaacaataaa ggttagtgag aagttatcaa aagtcgatgt
     5521 agataagttg ccgaaaaagg gtatagatat aactcacggc gataaagcct ttgaaaaaaa
     5581 gcttgaaaaa ttagaagaaa aataactata atcatttttt aaagttaaaa attttgaatt
     5641 tatggttaac atgtcaactt actatgtgta taatggtaaa cattgatatt aactatatgt
     5701 ataaaaatgt cacgcagatg ctatttaaat gtgataaata tttttagagg tgaatagagt
     5761 ggctataaag ctaagttcaa ttgaccaatt tgaacaggtt attgaggaaa ataaatatgt
     5821 ttttgtatta aaacatagtg aaacttgtcc aatatcggca aatgcgtacg atcaatttaa
     5881 taaattttta tatgaacgcg atatggacgg ttattatttg attgtccaac aagaacgcga
     5941 tttgtcagat tatattgcta aaaaaacgaa cgttaaacat gaatcacctc aagcatttta
     6001 ttttgtaaat ggtgaaatgg tttggaatcg agaccacggt gatattaatg tgtcgtcttt
     6061 agcacaagca gaagaataat gaaactatag ggttggaaca ttttgcctta cactactaga
     6121 cgtgaatagc acaacttaaa ttcgtgtgaa tcagagtagt ttggctataa tgatgttctg
     6181 accttttatt ttatgtaacc tttagaagca gttaagttag tactttt
//
LOCUS       DAPIDM010000142         6206 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000142 DAPIDM010000000
VERSION     DAPIDM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.142"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..615
                     /locus_tag="RJE42_002293"
     CDS             <1..615
                     /locus_tag="RJE42_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDV5877689.1"
                     /translation="LKEPKEYRKEWATWNLSSLPILPNYYEYKVSYDKREQFNFIKQL
                     FNDSSIRTIVNGCDSDREGSNIFYSSYYMTGAKNKEIKRLWINSLEVDEIRKGFNNLQ
                     DNKKDLLLYYEAKTRQISDWLVGMNGSRLFTLLLQQKGFNDSLSIGRVQSSTVYLIYQ
                     RQKEIEQFVSTPFYEIEGSFTAKNGMYKGKAKIKSETLKLQLML"
     gene            594..959
                     /locus_tag="RJE42_002294"
                     /pseudo
     CDS             594..959
                     /locus_tag="RJE42_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(892..1506)
                     /locus_tag="RJE42_002295"
     CDS             complement(892..1506)
                     /locus_tag="RJE42_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877690.1"
                     /translation="MKDETNNTGIRMFKRYAAITTSAKILSHVLATDIDIAKIRDYFI
                     DYHGHTVSERSLADKAIEVITQFVAQNRGKFSDDTALKNMIENHGLIALKDDYIEVKI
                     IASVFKDMLIENHFQDVNNVVNALKDKGFIESDRDRITTKRTVKDDNGKKQSLVFYHL
                     KLDPEYASIFGLTKDAEPIKAEINLDNKKMLESWKKQKLLIFLM"
     gene            1593..1868
                     /locus_tag="RJE42_002296"
     CDS             1593..1868
                     /locus_tag="RJE42_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV5877691.1"
                     /translation="MIDKLAKGAIDAETFRKQSQSLLQQSKPTLSINEQQIQRSFENV
                     IQQHFTLSMLYRYIDEIHISKNKSLVGIYFKNEPLNIVNQATQSSIA"
     gene            complement(1980..2339)
                     /locus_tag="RJE42_002297"
     CDS             complement(1980..2339)
                     /locus_tag="RJE42_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877692.1"
                     /translation="MDDRALASALGIGVGGGLAWIMEGFQSGHGFIYSLCAGLIIGLL
                     FFEMIPESIELGGWVILLGGIAIGLVLFQSIHLLMDKITIITNSHQKDIFVRSGVLLI
                     ISIAIHNFPVGNIPLFV"
     gene            2546..2872
                     /locus_tag="RJE42_002298"
     CDS             2546..2872
                     /locus_tag="RJE42_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV5877693.1"
                     /translation="MEEKKELEEVNNKDLDDETLFVVSQTFKALGDPTRIRILHLLFY
                     KEYSVNGIAETLHLRQSTVSHQLRFLKNLRLVKFRREGTTLFYSHDDEHTMNMLKQAI
                     DHACHH"
     gene            3202..5127
                     /locus_tag="RJE42_002299"
     CDS             3202..5127
                     /locus_tag="RJE42_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDV5877694.1"
                     /translation="MKNIQEQQAHESHSHDHSHDHDHGKMPIISYFIGLVLAIIGLFL
                     SDANLLIQNILFSIATITAGYHVIILEGIGETVENTKLKGKFTPNSHILMGLAAIGAS
                     LIGSFWEGTLLILIFSGAHFLEDYAEGKSKREITKLLEMNPTTAKLILPDGNTKIVDV
                     SELKVGDQLQVLNGDQVPIDGIILSGTTSIDESSINGESIPKEKSKGDEVFGSTINGT
                     GTFTMEVTKENKDTVFSKILQLVSQNQDNQTKAASIIQKFEPKYVNIVLIAIPLVMLL
                     APFLFDWTWSQSVYRGLVLLVAASPCALAAATVSVTLSTTSNLAKKGVLSKGSTYLSQ
                     LADIDAIAFDKTGTLTNGEPKVTNYYFTHSVNEENIIDIIVALEKESNHPLANAILEK
                     FEVKNKIDIEVTNQIGKGLTGDYNGKNYRIGKPTSFESASEEYTQFNHDWASEGKTVV
                     YVAENEEVIGIIALMDIPNEHAKETINYFKKLGIHTTLITGDSEMTGKAVGEQLGIDE
                     VIANVMPEDKSRIIEEQKEKFGVTAMVGDGVNDAPALVNADVGIAMGGGTDVAVEVSD
                     LVLMQNNLSKLVQSHKISSNMGRVIRQNIIFSMAVVAFLVVISLLGLTDITISVIVHE
                     GSTLVVILNGLRLLRSK"
BASE COUNT         2231 a          969 c         1101 g         1905 t
ORIGIN      
        1 ttgaaagaac caaaagaata tcgaaaagag tgggcaactt ggaatttgtc ctctttacca
       61 attctgccaa actactatga gtataaggtg tcttacgata agagagagca gttcaacttt
      121 attaaacagc tattcaatga ctctagcatt agaacaattg taaacgggtg tgattcggac
      181 cgcgaggggt ccaatatttt ttattcatcc tactacatga ctggagcaaa aaataaggaa
      241 attaaacgat tatggattaa ttcactagag gttgatgaaa taaggaaagg attcaataat
      301 cttcaagata acaaaaaaga cttattactt tattacgaag caaagacacg tcaaataagt
      361 gattggcttg ttggaatgaa cggaagccgg ttatttaccc tcctcttaca acaaaagggg
      421 tttaatgaca gtctctcgat tggacgggta cagtcttcca cagtatacct tatttatcaa
      481 cgtcaaaaag aaatagaaca atttgtttct acacctttct atgaaattga aggaagcttt
      541 accgccaaaa atggcatgta taaaggaaaa gcaaaaatta aaagtgaaac tctaaaattg
      601 cagctgatgc tctaactcgt gctaattaca agtgtgaata caatataaaa gataaaatct
      661 tcgaaagaaa atctggtaag ggttatacag agccacatca tttaataccg attagtaaat
      721 ataaagattt cgattataaa aattgtagtt tagatacaat ggagaacatc gtttcacttt
      781 gtagtcattg tcataattta cttcattatg gtaaattcga agataaaaaa cctattcttg
      841 aaaaactatt taatgaaaga aaaaaagcgt taagtgctgt tggcttagat attacattag
      901 aaaaattaga agcttctgtt ttttccatga ttcaagcatc tttttgttat ccaagttaat
      961 ttcagcttta ataggttcag catctttagt tagaccaaaa atagacgcat attctggatc
     1021 tagctttaaa tggtaaaaga caagtgactg ttttttgcca ttgtcatctt tgactgttcg
     1081 ttttgtcgta attcgatcac ggtcagattc gataaatccc ttatccttta gtgcattaac
     1141 aacattattg acatcttgga agtgattctc tatcaacata tctttaaata cagatgcaat
     1201 gattttgact tcgatataat catcttttaa ggcaattagt ccatggttct caatcatatt
     1261 ctttaacgct gtatcatcag aaaacttacc acggttttgt gctacaaatt gcgtaatgac
     1321 ttcgatcgct ttatctgcca gtgaacgttc agagactgta tgaccatgat aatcaataaa
     1381 gtagtctctt attttagcga tatcaatatc tgtagctaaa acatgactaa gaatcttggc
     1441 cgaagtagtg atagccgcat agcgcttaaa catacgaata ccagtattat ttgtttcatc
     1501 cttcaattta tctttaaacc aatgatgtaa ttcaaaaacg gatcaaaaaa caaagggccc
     1561 tagaaactag gagcacactt acacaagaac aattgataga taaattagct aaaggagcca
     1621 tcgatgcaga aacgttcaga aaacaatctc aatcgttact tcaacaatca aaaccaacac
     1681 tatcaataaa tgagcaacaa attcaaaggt cttttgaaaa tgtaattcaa caacacttca
     1741 cgttaagcat gttataccga tatattgatg aaattcatat ttctaaaaac aaaagccttg
     1801 tgggaatcta tttcaaaaat gaaccgctaa acattgtaaa ccaagctacg caatcatcga
     1861 ttgcttaatt aatgaaagaa tgaaaaatat gaaagaaatg aaaagaaaat ctgtaggatg
     1921 ttatgttaga gtttcaacaa tttctcaaga cattgataaa tttagtatta atggtcaaat
     1981 tacacaaata aaggaatatt gccaacaggg aaattatgaa ttgctatact gataattaaa
     2041 agtacccccg atcgaacgaa aatatctttt tgatgactat tagtgataat tgtaatttta
     2101 tccattaata gatgaatgga ttggaataat actaagccta ttgctattcc accgagtaaa
     2161 attacccacc cgcctaattc aatactttcc ggaatcattt caaaaaataa taagccaatt
     2221 ataaggcctg cacataatga ataaataaag ccatgaccac tttgaaaacc ttccattatc
     2281 caagcgagtc caccgccaac accaattcca agtgcggaag ctaacgcccg atcatccata
     2341 ctgcactcat cccactattc ttaaaaaggc tttgtttatt ttactctaaa aaatcaaaac
     2401 aaaatatatg aattcatatt aaatattatt tgactcttta ttcagatggt tatatcatat
     2461 atatgaacat atattaatat atgtagataa atagtgatta ttatatataa ggttttgaat
     2521 tgaaacctaa agtgagggaa ggataatgga agaaaaaaag gaattagagg aagtaaataa
     2581 taaggactta gatgatgaaa cattatttgt cgtatcgcaa acatttaaag cgttaggtga
     2641 tcctacgaga atccggattc tccatttgct cttttataag gagtattcgg taaacggtat
     2701 tgctgaaacg ctacatctta gacaatcaac agtttcccat caattgcggt tcttgaaaaa
     2761 tttacggtta gtaaaattcc gaagggaagg cacaacattg ttttattccc atgatgatga
     2821 acatactatg aatatgctaa aacaggcgat cgatcacgcc tgtcatcact agtattccgt
     2881 aaaccagtaa taagtgttaa taataattca cgtcattaat attgttatat gatcatataa
     2941 caatattata ggcatatgaa aagggggttt tgcaatggat atggaaaaca aaaaaacaga
     3001 atggaaagcg ttgtatgata tctcgaagga aagtgagatg ggtgtagcag agagggttag
     3061 tgaatacggt tgataaattc ataggatgta ttattggata caaaaagttt ggatgtggtc
     3121 aattaacgtc ctggtttagt aaagcttact gcgaatatat tggaacccga tatatcatat
     3181 ttatttagaa aggtgataga aatgaaaaat attcaagagc aacaagcaca cgaaagtcat
     3241 agccacgatc atagtcatga tcatgatcac ggaaaaatgc caattatttc atattttatt
     3301 ggcttagtgt tggctataat tgggcttttt ttaagtgatg caaatttatt aatacaaaac
     3361 atcttatttt caattgccac aatcacagcc ggctaccatg taattattct cgaaggaatt
     3421 ggagagacag ttgaaaatac taaattaaag ggaaaattca ctcctaattc tcatattcta
     3481 atgggattag ctgcaatcgg ggcttctctg atagggagtt tttgggaagg aacccttttg
     3541 atacttattt tttccggcgc tcattttctt gaagattacg ctgaaggaaa aagtaaaaga
     3601 gaaattacta agctactcga aatgaaccca acgacagcta aattaatcct acctgatgga
     3661 aacacaaaaa ttgttgatgt cagtgaatta aaagttggag atcaactcca agtgctgaac
     3721 ggtgatcaag ttccaattga tgggattatt ttatccggta ctacctcaat tgatgaatct
     3781 tctattaatg gagaaagtat accgaaagag aagtctaagg gtgacgaagt ttttggaagt
     3841 acgattaatg gaacaggtac ttttactatg gaagtcacta aggaaaacaa ggatactgta
     3901 ttctctaaaa ttttacaatt agttagtcaa aaccaagata atcaaacaaa agctgccagt
     3961 atcattcaaa aattcgagcc taaatatgtt aatatagttt taatcgcaat accattagta
     4021 atgttacttg ctccttttct atttgattgg acatggtcgc aaagtgttta caggggatta
     4081 gtgcttttag tcgcagcttc accgtgtgct ttggcagcag ctactgtatc tgtaacattg
     4141 tctacaacat ctaacctagc taaaaaaggc gtgctttcaa aaggaagtac ttacctatca
     4201 caattagcgg atatagatgc aattgccttc gataaaacag gaacccttac gaacggagaa
     4261 cctaaagtaa caaattacta tttcactcat tctgtgaacg aagaaaatat tattgatatt
     4321 atagtcgccc ttgaaaagga atccaatcac ccactcgcta atgctatttt agaaaaattt
     4381 gaagttaaaa ataaaataga catcgaagtt actaatcaaa ttggaaaagg tctgacagga
     4441 gattataatg gaaaaaatta tcgtattggt aagcctactt cttttgaaag tgcttctgaa
     4501 gagtataccc agttcaatca tgattgggca tcagaaggaa agacggttgt atacgtagca
     4561 gaaaatgaag aagttattgg gattatagct ctaatggata ttccgaatga gcatgctaaa
     4621 gaaacaatta attactttaa gaaacttggt atccacacga ctttaattac tggtgattcg
     4681 gaaatgacgg gaaaagctgt aggcgaacaa ttgggaatag acgaagttat cgctaatgta
     4741 atgcctgaag ataaatccag aattatagaa gaacaaaaag aaaaatttgg agttactgcc
     4801 atggttggag atggtgtgaa cgatgcaccg gcccttgtta atgctgatgt tggtatagct
     4861 atggggggcg gtactgatgt ggcagtagaa gtatctgatt tggttttaat gcagaacaat
     4921 ttatctaaat tagtacagtc tcataaaatt tcctcaaata tgggtcgtgt tattaggcaa
     4981 aatattattt tttcaatggc agttgttgcc tttttagttg tcattagttt gttaggatta
     5041 actgatatta caatcagtgt aattgttcat gaaggaagta ctttagttgt tatactaaat
     5101 ggacttcgat tattaagatc taaataatga acgaatcgat tgacatgaat gaactttgaa
     5161 gtgtggattc tacaatgttc ccataacatt ggacactaaa aaacagagca atctaataaa
     5221 gatgttatga gtaaaaacaa tgccttgcat accgttttta tcatacgggc ggtcaaataa
     5281 gcaattaaag agcatgggaa gtataaaatc ataaacagtg acttaaggca gccagtttac
     5341 attcaaaagt taaattgact gtattaaaag tttcgaaacg attaatatcc gtatgggggc
     5401 aagcgtcgag ccaaagataa tgccaggaca gagcgtcttt ttccgttctt ttaagtggga
     5461 aaggttttac cttctctatg ccaagacagt cccagagcta aaagggccag ttgctctttt
     5521 tgggcgtttg tgaacggtaa tcgcaggaag gggattttcc caaccgcacc tggttttcca
     5581 tcacaataaa gttggcggca tttgttctga tattcttggg aatacttaca ctatgaatgg
     5641 ccgtattaag tgatacagga gctgctatta ttgtaatact gaatgctctc cgccttttga
     5701 gggtaaaaga ataaaagtaa ggataactag gtaaagctgt tcaatcaaaa attgaacagc
     5761 ttatttttca tcaaaatcaa aaacgtttat tataatacct acacttgttc tgttaaatgt
     5821 accgattttt taaccttatt gtatcagtaa tatcttgaaa cgaagtaagc gactaaaatt
     5881 tctcttttat catacattta taaaaataca cttttaagaa cggtttgaaa atttttgaaa
     5941 tagatcaaaa taatcttctg gttaaaaaac ctgtagatat gatttctctc ttaaattttt
     6001 gtttgattag attagaccct aataagacgc attcaatatg tctacacgtg aatttagtct
     6061 ttgaaaatgt aaggaccatt attattataa aaaccccagt ataaaacgat acgctgaata
     6121 cagcaataaa taaaaaaagg catgtactgt gtaaaatgtt tgttaaatta tataaatatt
     6181 ttttaaatag atatttattc aataat
//
LOCUS       DAPIDM010000143         6205 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000143 DAPIDM010000000
VERSION     DAPIDM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.143"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..991)
                     /locus_tag="RJE42_002300"
     CDS             complement(<1..991)
                     /locus_tag="RJE42_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV5877695.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGEALQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQTGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLF"
     gene            1576..2631
                     /locus_tag="RJE42_002301"
     CDS             1576..2631
                     /locus_tag="RJE42_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDV5877696.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSVLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            2631..3527
                     /gene="murQ"
                     /locus_tag="RJE42_002302"
     CDS             2631..3527
                     /gene="murQ"
                     /locus_tag="RJE42_002302"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV5877697.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEYDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            3539..4993
                     /locus_tag="RJE42_002303"
     CDS             3539..4993
                     /locus_tag="RJE42_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV5877698.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDQIPHNHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            4996..5871
                     /locus_tag="RJE42_002304"
     CDS             4996..5871
                     /locus_tag="RJE42_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV5877699.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
BASE COUNT         2069 a          974 c         1258 g         1904 t
ORIGIN      
        1 cgaataagaa tgagaattct aaaggttctg taataccagt taagaatgat gttaaagcag
       61 cagaacccat taaaccggct actacttttt tgttttcagg tttagctgtg tgataaattg
      121 ctaaagctgc tgcaggtaaa ccgaacatca taacagggaa ttcaccttgc atgaatttac
      181 cagctgtcaa atgtgcacct tcacgaattt gttcgataaa gatacgttgg tcaccgtgaa
      241 taatttcacc agctgcattt ttccatgaac caaactcgaa ccagaacggt gcgtggaaaa
      301 tgtgatgtag accgaatgga attaataaac gcttaataaa accaaataag aatacggcaa
      361 caccagtatt tgagtctaat aatcctgtac tgaatgcatt taatcctgtt tggattgttg
      421 gccagattaa tgccattggg aatgctaaaa taaatgatgt tgtagccatc ataataggta
      481 cgaaacgctt accagcgaag aaacctaaat aagatggtaa gttaatgtta tagaacttgt
      541 tataacacca agctgccagg gccccgatta taataccgcc gaacacacct gtttgtaatg
      601 ttgggatacc taaaatgcta gcgtaaccac tcgctggatc accaatgttc ttaggtgtta
      661 cttgtaaaaa gtcacccatt gttttgttca tgattatgta accgacgaat gctgcgatag
      721 ctgctacacc atcaccgcca gctaatccga ttgcgacacc taatgcgaaa atcataggca
      781 agttatcaaa aatgatacca ccagcacctg tcattaattt agcgacagtt tgtacgccac
      841 cattttgtat aaacggcaag tagtgttgta atgcttcgcc ttgcatagct gtaccgatag
      901 ctaataacag acccgctgct ggtaaaatcg caacaggtaa cattagtgcc ttaccaatac
      961 gttgcaattg accgaaaagt ttcttcctca cttgtccaac ctccaaagtt gtatttatta
     1021 tttaagattc aataaaaaag acacgagcaa atagactgtg ttggtaacca tcacagctta
     1081 atttaactca tgcctaatct tacttagtaa cacgttggtg tatgtaatta atgtaaaaga
     1141 agcaattgac taagtagcat ataaaactga tacgttagtt tatctagctt accatcacat
     1201 cttattgaat cttatttgtt tgcgactcct attttagcat ggtgaagtat gcgttttcaa
     1261 tacaattatt aaattcaatt tcaaatctct taattgttca cagattatta ttaaaacata
     1321 atataagata atcctgaata aaatgtataa aagttgttgt aataatccag caatagcaac
     1381 tgttttcgag cgttaaatga taagaaaaat atgtcgtttt aaaaaattaa cttgtgacag
     1441 aaatatgtaa gattattaca atgaacaggc ttcaactaat taatattgta atgtgttttc
     1501 tcttatagta aaatgtaagc gattacacaa ctcaactata ttttcctaat aattatattg
     1561 ttaaggaggg ctactttgac aggcttttca gtgtatttag gacaaccttt agatgaagcg
     1621 tatattaagc gaatgattaa acaaggttac caaatgattt ttacatctgt acaaatacca
     1681 gaagaagatg acgagacaaa atatcattat ttcacaaaac tactcaattt attaaaacat
     1741 gaacaagtga cttacctcat agatgctaat ccatctatat taacaccatc tttttatgag
     1801 catcttcgac aatatgatgc acaatttatg attcgtatcg atcatagtac atcaattgag
     1861 gcaatcgaag cgataatggc acagggttta aagtgctgtt tgaatgcaag tattatttcc
     1921 cgggaattgt taacaagctt acatcaacaa ttgaatgatt ttacattact ttcattttgt
     1981 cataactatt atccaagacc agatacggga ttatctgttg acttggtcaa taagaaaaat
     2041 gaactcattt atcaatttaa tccaaaggca caaatatatg gttttattgt agggagtgtt
     2101 ttgcgaggtc ctttgcataa aggcttgcca acaattgaag caacgagaca tagtcatccc
     2161 gtcgttgcag ctaaattatt acaagaaact ggtgtatctg aagtgttagt tggagactca
     2221 ttgattgaaa tgaggcaggc aaaacaactt atagattttt gcaagcatag gcatttcacg
     2281 ttatgtattg aagaagtgtt tgatacgaca gtgacttacc ttttcgatat gtgtcataaa
     2341 gtacgtccgg ataatccgga gaatgtcatt cgttcggaaa cgtcaagaca aatatgtccg
     2401 cattcgattc aaccacagtt tacgacgcaa cgacgcattg gttcagtaac cgttgataat
     2461 ttgaataacg gacgttatca aggtgaaatg caaattgtga gacaaacgct tagtgcacat
     2521 gacaatgtga atgttgttgc acaaattatt aaagaagact taccactgtt aagttgtatc
     2581 gagccgaatg atacatttga ttttcaaaaa actagggagt gtaagaagtg atggaaaata
     2641 gtacgaccga agcgcgtaat gaagcgacga tgcatcttga tgaaatgact gtggaagagg
     2701 ctttaattac gatgaataaa gaagatcagc aagtcccgtt agcagttcga aaggcaatac
     2761 cacaattgac aaaagtaatt aaaaaaacaa ttgcacagta taaaaagggt ggacgattga
     2821 tctatatcgg tgcaggtaca agtggaagat tgggtgtctt agatgcagcg gagtgtgtac
     2881 ctacattcaa tactgaccct catgaaatta taggtattat tgctggtgga caacatgcta
     2941 tgacaatggc tgtagaaggt gcggaagatc acaaaaaatt agcggaagaa gatttgaaaa
     3001 atatagattt aacatcaaaa gatgtcgtta taggaattgc cgcgagtggc aaaacgccat
     3061 atgttatagg cggtttaaca tttgctaaca caattggtgc tacaacagta tctatttcat
     3121 gcaatgaaca tgcagttata agtgaaattg cgcagtatcc agtagaagtt aaagttggtc
     3181 cagaagtatt aactggttca acgcgtttaa agtctggtac agcacaaaaa ttaattttaa
     3241 atatgatttc aaccatcaca atggttggtg tcggaaaagt ttacgataac ctcatgattg
     3301 atgttaaagc aaccaatcaa aaactgatcg accgttcagt gcgtattatt caagaaatat
     3361 gtgctatcac atatgatgaa gcaatggcgt tatatcaggt atctgagtat gatgtgaaag
     3421 ttgcgacagt tatgggtatg tgtggcattt ctaaggaaga agcaacaaga cggttattaa
     3481 acaatggtga cattgttaaa cgagcaatca gagatagaca accttaggag ggatttaaat
     3541 gaccaaagaa caacaacttg cagaacgaat tattgctgca gtaggtggta tggataatat
     3601 agatagtgtc atgaactgta tgacacgtgt gcgtattaaa gtattagatg aaaataaagt
     3661 agatgaccaa gaactaaggc acattgatgg tgtcatgggt gttatacacg atgaacgcat
     3721 tcaagttgtg gttggacctg gtacagtcaa taaagtggct aatcatatgg cggaattaag
     3781 tggtgttaaa ctaggtgacc aaataccaca caatcacaat gatagtgaaa aaatggacta
     3841 taaatcatat gcagctgata aagcaaaggc gaataaagaa gcacataaag caaaacaaaa
     3901 gaatggtaag ttgaataaag tattgaaatc aattgccaat atctttatac cattgattcc
     3961 tgcatttatt ggagctggat taattggtgg tattgcagca gtactgagta acttaatggt
     4021 ggcaggctat atttcaggtg cttggattac gcaacttata acagtattta atgtcattaa
     4081 agacggtatg ttagcatact tagctatttt cactggtatt aatgcggcta aagaatttgg
     4141 tgcgacacca gggcttggtg gcgtgattgg tggtacaacg ttattaacgg gtattgctgg
     4201 taaaaatatt ttaatgaatg tcttcactgg agaaccattg cagcctggac aaggtggaat
     4261 tattggcgtt atttttgccg tttggatttt aagtattgtt gaaaagagat tgcataaaat
     4321 tgtgccaaat gcgattgata ttattgtaac gccgactatt gcattgttga ttgtaggact
     4381 attaactatc tttatcttta tgccattagc aggttttgtt tcagacagtt tagtttcagt
     4441 agttaacgga attattagta ttggtggcgt atttagtgga tttatcatcg gtgcaagctt
     4501 cctaccatta gttatgttag ggcttcatca tatttttacg ccaattcata tagaaatgat
     4561 taaccaatct ggtgctactt acttattgcc aattgcagcg atggctggtg ctggacaagt
     4621 aggtgccgca ttagcacttt gggtaagatg taaacgcaac acaacattac gtaatacttt
     4681 aaaaggtgca ttgccagttg gtttcctagg tatcggagaa ccattaatct atggtgtgac
     4741 tttgccatta ggtcgacctt tcttgactgc ttgtattggc ggtggtattg gtggcgctgt
     4801 aataggtgga attggacata ttggtgccaa agcaataggc ccaagtggtg tgtcactctt
     4861 accattaatc tcagataata tgtatttagg ttatattgca ggattacttg ctgcgtatgc
     4921 tggtggattc gtttgtacat atttatttgg aacgacaaag gcgatgcgac agactgattt
     4981 gttgggtgat taatgatgac aaatatttta tatcgcattg ataagcagtt gagtgatttt
     5041 acgaagacag aaaagataat cgctgattac attttaaaga atccacataa aatcattgat
     5101 atgaccgtga atgatttggc agatgttacg aatgttagta cagcatcaat tgttagattc
     5161 agtcggaaaa tgacacacca aggttttcaa gagctaaaga ttgcgatatc tcgatactta
     5221 cccgaagata ttgcaaccaa tccacatttg gaattgattg aaaatgaatc tgtagaaact
     5281 ttgaaaaata aaatgattgc tagagcaacg aatacgatgc gatttgtagc tactaatatt
     5341 atggatgcgc aaattgatgc aatttgtgat gtgttgaaaa atgccaggac aatattttta
     5401 tttggatttg gcgcatcgag tttgactatt ggtgatcttt ttcaaaagtt atctcgtatt
     5461 ggcttaaatg tcaggttatt acatgaaacg catttacttg tgtcaacatt tgcgacacat
     5521 gatgatagag attgcatgat ttttgtgacg aatcaaggta gtcatagtga attgcagtca
     5581 attgcacagg tggccacaca ttacagtatt cccatcataa ctatatctag tacagctaat
     5641 aatccagtgg ctcaaattgc agactatgca ttgatttatg gcagaactga tgaaaatgaa
     5701 atgcgtatgg cggctacaac gtcactattt gcacagttat tcacggtaga tatattgtac
     5761 tatcgatttg tagcattaaa ttatcatgcg attctagatt gtataaccca atcgaaaatg
     5821 gcacttgata attacaggaa gcatcttgcg acgatagatt ttaaacatta gatattgtgt
     5881 gcgttctaac ataaagttag ttgcaacgta aacaattgaa gtttttgatg tgattcagtt
     5941 gcttgatgaa aaattgttgc tacaagtttg taaaactggc atagaggcta ttttgaaaat
     6001 gataaaggcg tgacaaatta aagcaaagtt cagatttgag ctttgctttt ttcatagatg
     6061 gaaataaaat gctgttatta aggggggaat taatttggaa cttaacgaga tgattaattt
     6121 tgtaaaagaa aacaataaga taatggttac tttatcgcta acaattttca cgtctgtaat
     6181 atttcctgag ttaaaaagtt ggtat
//
LOCUS       DAPIDM010000144         6190 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000144 DAPIDM010000000
VERSION     DAPIDM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.144"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            139..1332
                     /gene="fmtA"
                     /locus_tag="RJE42_002305"
     CDS             139..1332
                     /gene="fmtA"
                     /locus_tag="RJE42_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDV5877700.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIGLVFHRLQTKTNSIEPIHKETK
                     LSDNAKYLVDRNKGKGEPSKLKEVYNSKDPKYKKIDKYLQNSLFNGSVAVYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDSVSKYIPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVRAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHSAFYDEKSFRKYFAKGYSYNSTGLSFLKP
                     NVLEQYYGAGNIYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1676..1954)
                     /gene="qoxD"
                     /locus_tag="RJE42_002306"
     CDS             complement(1676..1954)
                     /gene="qoxD"
                     /locus_tag="RJE42_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDV5877701.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(1963..2568)
                     /gene="qoxC"
                     /locus_tag="RJE42_002307"
     CDS             complement(1963..2568)
                     /gene="qoxC"
                     /locus_tag="RJE42_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDV5877702.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2558..4546)
                     /gene="qoxB"
                     /locus_tag="RJE42_002308"
     CDS             complement(2558..4546)
                     /gene="qoxB"
                     /locus_tag="RJE42_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDV5877703.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4546..5646)
                     /gene="qoxA"
                     /locus_tag="RJE42_002309"
     CDS             complement(4546..5646)
                     /gene="qoxA"
                     /locus_tag="RJE42_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDV5877704.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            5951..6070
                     /locus_tag="RJE42_002310"
     CDS             5951..6070
                     /locus_tag="RJE42_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877705.1"
                     /translation="MERKRKHFNEEFAKSNLSLNNEKWKFVCSKSKHSFKKSS"
BASE COUNT         2239 a          965 c         1063 g         1923 t
ORIGIN      
        1 agaacttata tgaagaaaat attactaagt attataacgt ataagtgggt aacagataaa
       61 attgtaagtg atattataac gaagttaagg ttattgtttt aattatttaa tttgaataca
      121 atgagaggct attagcgaat gaaatttaat aaagtgaaat tggttataca tgcgtgcgtg
      181 cttttgttca tcattatctc tataggatta gtttttcatc gattacaaac gaaaacgaac
      241 tctatagaac ctatacataa ggaaacaaaa ttatcggata atgcaaaata tttggttgat
      301 cgtaataaag gcaagggtga gccatcgaaa ttaaaggaag tatataatag taaagatcct
      361 aagtataaga agattgacaa gtatttacaa aattcattat ttaatggttc agtagcggta
      421 tatgaaaatg gcaaattgaa aatgagtaag ggctatggtt atcaagattt tgaaaaagga
      481 attaaaaata caccaaatac gatgtttcta attggttcag ctcaaaaatt ttcaacagga
      541 ttactgttaa aacagttaga agaagaacat aaaattaata ttaatgattc agtaagcaaa
      601 tatattccat ggtttaaaac atctaagcct attcctttaa aagacttgat gctgcatcaa
      661 agtggattat ataaatataa atcttcaaaa gactataaga acttagatca agcagtgaga
      721 gcaattcaaa aacgtggcat tgatcctaag aaatacaaaa agcatatgta taatgatgga
      781 aattatttag tgctagctaa agttatcgaa gaagttaccg gcaaatcata tgccgagaat
      841 tactatacta aaataggtga tccattaaaa cttcagcatt cggcatttta tgatgaaaaa
      901 tcatttagaa aatattttgc aaaaggttat tcttataata gtacaggact ttctttcttg
      961 aagccaaatg ttttagaaca atattatggt gcaggtaata tatacatgac accaacagat
     1021 atgggtaaat taattactca gatacaacaa tataaattat ttagtcctaa aataaccaat
     1081 ccattattac atgagtttgg tacgaaacag tatccagatg aatatcgata tggtttctat
     1141 gttaagccaa cattgaatag acttaacggg ggattctttg gacaagtctt tactgtttac
     1201 tataatgata agtatgtagt tgtacttgca ttaaatgtaa aaggaaacaa tgaagttcga
     1261 atcaaacata tttataatga tattttaaaa caaaataaac cttataatac gaagggtgtt
     1321 attgttcaat aattaatata gaagatataa cttgtatatg gcattaaggc attgacctta
     1381 tttgactagt atacgagtta tatcttcttt tttatagagg tgaaaagttt aaaggataag
     1441 attgaagtag tagggtgata aatttgcgtt aagttaaaaa atatataaac aattgatgtg
     1501 aatgtcaata taaaaatgta cataaatgat tgtataagaa tgtattaatt gaatggataa
     1561 taatgattgt cttattagat atagaagggg aattaaccat ttaaaaattg aaggataata
     1621 aaaaccagct aacaaaagtg ttagctggtt aaactttaca ttattaaaat gttgtttata
     1681 agtgtgaaga gtgaccgcct tgcataaccc aatatgttcc gacaacgaaa caaagtgtaa
     1741 ttacaagagc aaagataact ttgaatgttt gtaaacgtcc atctttacct tcagttaaat
     1801 gcatgaacat taataattga agtcctgctt ggacgaatgc aaagccaaag ataattgtca
     1861 acttcgcgtg gaatgttaat gacgtgtata gtgttacgta aactgctaaa agcgttaata
     1921 cgatagatgc gataaatcct acagtatgtt tcattattgt actcatccgc tatacaccat
     1981 ccctatcata tatacggcag taaagatgaa aacccaaaca acatctaaga agtgccagta
     2041 taaacttact ataaataatt ttggcgcatt atatttgtct aatccgcgtc gttggatttg
     2101 gattaataaa caaatggccc aaacgatacc tagcgatacg tgacaaccat gcgttcctaa
     2161 taggataaag aaactagacc agtaagaacc aattgttggg ttaacgcctt ctgatgcata
     2221 gtgtgcgaat tcataaattt cgaatccaac aaagactaaa cctaaaagta acgtaatgat
     2281 catccaaaac atcattaact tttgtttttc ttggcgcatg tagtaaatag caataccaca
     2341 tgtgtaagaa ctgaataata atgcaaacgt cattattaaa acaagaggca attcaaataa
     2401 ctcagtagtc attttacctg cataatcgcc accatgttgc aaagttaata gtgtcgcaaa
     2461 tagggtaccg aataacgcaa attcggctgt aatgaaaatc caaaagccaa gcttatttaa
     2521 ttcgccttca tgtgtgcgtg aatcaatagt gtttgtatca tgactcatga cttacagcct
     2581 ccctttcttt aatacgcgct tctcttaatc tagcttcagt ttcagcaact tcagcagcag
     2641 ggatatgata tccgtgatcg atttggaaac tgcgataaat catagtacca aaaataccga
     2701 ataaacaaat taatgctgga attacagttt cgaaaattaa gaagaaaccg ccgatagtca
     2761 taaagatacc aatccagaat ccaacaggag tattgtttgg catatgaatg tctttgtaat
     2821 tatggttgtc taaataatga cgaccatgtt ctttcatatc aacaaatgtg tcgtagtcat
     2881 tccaatctgg tgtaatggca aagttgtatt taggtggaat tgctgatgca gtagtccact
     2941 ctagagtacg accaaggcca tcccagttat ctccagttgc ttcacgtgga gatttgaagt
     3001 gactgtatac gatactaaca acaaggaata agaatccgat tgccattaat aatgcaccga
     3061 tagttgaaat taagtttaat aagaaccaac catctgatgg catataagtg tataaacgac
     3121 gtggcatacc atctaatcca agaatgaatt gtggtaagaa acaaacgtta aatccgatca
     3181 tgaagaacca gaagcaccat ttgtttaatg tttcgtttaa tttgtaaccc atcatctttg
     3241 gataccagaa gattaaacca gctaagcagg caaatacaac accagtaacc aatgtatagt
     3301 ggaagtgagc tactaagaag tacgtattgt gatattgata gtcagcagat gccattgcta
     3361 acattacacc cgtaacacca cctaaaagga agttagggat aaatgctaat gagaataaca
     3421 ttggtgactc aaatgtaata cgtcctttat ataatgttaa taaccagtta aacaatttca
     3481 caccagttgg aataccaatc agcattgttg aaattgagaa gaatgagttg attaacgcac
     3541 cattacccat tgtgaagaaa tggtgaaccc aaactaagaa actaaggaac gcgataccgg
     3601 cagttgccca taccatactt tgatgtccga ataaacgctt acgagcgaat gtcggaataa
     3661 tttctgagta aataccaaat gctggaagga taacgatata aacttcaggg tgcccccata
     3721 cccagaagaa gttagcccaa agcattggca taccaccatg tgcaactgtg aagaatgctg
     3781 tgtcaaatat tctatcagtt gtcattaatg ctaacgctac tgttaaagga gggaaagcaa
     3841 gaataacaat taatgttgta ataaatgttg ttactgtaaa cattggcatt tgcataaact
     3901 tcatagttgg tgttttacat cttaaaattg ttacaaagaa gttgatacct gtagctaagg
     3961 taccaagccc tgaaatttgt atagctatta agtaatagtt aacacccgga ccaggactga
     4021 attcacctgc tagtggcgca tagtttgtcc aaccagctgc tggtgaacca ccaataataa
     4081 atgacaggtt gaataaaatc atacctgcaa agaatagcca gaaacttacg ttgtttaata
     4141 ctgggaatgc aacatcacgt gctccaattt gtaatggaac aacgatattc cataaaccaa
     4201 agataaatgg cattgccatg aagataatca tgattacacc atgtgtacta aaaatttcgt
     4261 tatagtggtt agattctaaa aatttgttat caggtactgt taattgcgca cgaataagta
     4321 acgcatcaat accaccacgg acgaacatta atacggcaca gattaaatac ataataccga
     4381 ttttcttatg gtctacagat gtgaaccatt ctttgtaaag atatttccat aatttaaagt
     4441 aagtaattac tgcgattaaa ccaataacta agaatggggc accaatttgt gccattgtaa
     4501 tcatccagtt acctttaact agtaattgat cccatggaaa attcattaat gtccacctcc
     4561 atgatcatca ttgtcttgat cttgcgcatc ttttgaaatt ttcttcattt ctttcgcatt
     4621 tttcgattca tctttcttga actcattgtt atatggttcg tcatttccaa gaatcatcaa
     4681 tttcatacca tgtcgtttat agttcgcatt tgtaatttga gctttacgag caggtattaa
     4741 tggtttgtct gatacatctt taaacatatt ttcttcacta gtgaagtttg gatctttcaa
     4801 ttcgaaattg aaacgtttat atgcatagaa gatgtattct ggatcggctg ctggatcaac
     4861 aaacgccata tgtgtaccat taaattctaa agctttatta ggtgtgcttg gtaataattg
     4921 tttatcaaat gtatcttgat ctaacgtttt cttaccttta acttctttca cccatttgtc
     4981 gtagtctttt tgactaacgg catttacttt aaatgtttga cgcgagaatc cttcaccatt
     5041 gaagttagag ttacgacctc tgaacgtacc agtttgagat gcttctaacg tccaattcat
     5101 tgtcatgcca gtcatggcat atttttgacc acctaattgt ggaatccaga aacttgtcat
     5161 tgtatccata gcttgaagct taaatacaac aggacgatct ttagggattg ttaatgtatt
     5221 aacagtctct atatgttcat ctggataagc aaagaaccat ttgtatcctg cacttactgc
     5281 atatacaacc attggatctt tctcactctt cggtggtttt tcgtaatcgt ataaagtttt
     5341 aactgtagga atagctaaag cagcaacgat tatgataggt attacaaacc atattgtttc
     5401 aatgatggca ttatggtgca tcttaccaga ttcggcattc ttattataac tatacttgta
     5461 aataaaaatg gcgaacatgc caagtacaac gaaacaaata acaagcatga agacgattga
     5521 ataaagaatc aagaacttct gactacttgc tactggccct tttgcgttga aaatttctat
     5581 atttgaacaa ccactaagta aaattagtgt gccaaataat agaagcaaag acttaaattt
     5641 tgacactttt ttgacctcct aatactacaa atgtagggct taacattaat tttaagttat
     5701 tacacaatat ttacaagggc ttatgggaaa aaaattaata aaattgtatc aaaaatgttg
     5761 ataaatcaag gtatgacgtg ggttcacaca tttgttaaaa ttatgtgtac attttgtgac
     5821 taatagcgtt tttgaaaaaa ttgcttcata tttaaaagga aagttgagaa tagctttcaa
     5881 ttaaaggtta attgaatgaa aataaaaaaa gtttgaaatt gtctaataaa ttgaaaatga
     5941 tggctagttc attgaaagga agcgcaaaca ttttaatgag gaatttgcca aaagtaattt
     6001 atcattaaac aatgaaaagt ggaaatttgt ttgttcaaaa tcaaaacatt cttttaaaaa
     6061 gagcagttaa gttttaataa tttgataggt agtcctttgg tatacagaaa aattacaata
     6121 tttgggttaa agttgcagat gaagttggaa taacaaataa aatggtagca aaacatgtac
     6181 gtttgctacc
//
LOCUS       DAPIDM010000145         5950 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000145 DAPIDM010000000
VERSION     DAPIDM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.145"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..750
                     /locus_tag="RJE42_002311"
     CDS             <1..750
                     /locus_tag="RJE42_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5877706.1"
                     /translation="KLTLGICLVLLIILIVGYVIMTKTNGRNAQIKEAFNKTLNVYPT
                     KNLEDFYDKEGFRDQEFDKRDKGTWIINSGMNIQLKGGALKSRAMVLYINRNTRTAKG
                     YFLISETTEDKKGYVHNKDKKYPVKMEHNQIIPTKPIRDDKLKKEIENFKFFVQYGDF
                     KDYKDGDISYNPNVPSYSANYQLNNDDYNVQQLRKRYDISTKRAPELKLRGSGDLKGS
                     SVGSKELEFNFIRNKEENVYFTDSINFKPTE"
     gene            998..1783
                     /locus_tag="RJE42_002312"
     CDS             998..1783
                     /locus_tag="RJE42_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5877707.1"
                     /translation="MIHSKKLKLCLCLIILSVFIGGCGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMIIETNGRNMESRGMVLYINRNT
                     RTTKGNFVIREITEDSKGYSDSKDKKYPVKMENNRIVPMKPIHDEKLRKEIEDFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLLK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTPSEDNNL"
     gene            1780..3135
                     /locus_tag="RJE42_002313"
     CDS             1780..3135
                     /locus_tag="RJE42_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877708.1"
                     /translation="MSQTEYQIKPGNIKANSEETSTVSKISYEIENANNSGLKKGKIN
                     EQIEGLKEEGKFPKNLKYLKSYTDPNTGTTTTAFKNKDTGKVTLGMTGTNLHFEEMGN
                     VLKHPFNHSDQDMKDMKEMLKDFGADANIGLGAVTDKDPHFKDTQDFIKDIKKEYEID
                     TITGHSLGGRDAIILGVSNDIKNIVVYNPAPLSITDFRIIALNNMTYSAIGDIEIKEM
                     LKNYNGHIVRFVSDKDELDGFVSQFMYDTAGEKIVLNNGEGHDMGAFLRKYAQAKILA
                     ELRKVKGYEDANNKSFKSVKDNTKSKLGKVEELRVNWLQANGGALTSSQLKLLESVSA
                     LIIAEGLSQLVKEESNQLEKMYDNMKDKFQENWKNSQEAGNKIGTKLSHDGVLTALRN
                     GDAYESKFETEPLTKIEQKLKELNDINSDCHNYVSKIKDSVNAIVGNDQLLASQIAGV
                     I"
     gene            3135..3461
                     /locus_tag="RJE42_002314"
     CDS             3135..3461
                     /locus_tag="RJE42_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877709.1"
                     /translation="MFTEYKKLSDLETAYDEERRKLNDKLEQLQEIKHQIKLDCEYSY
                     DCFLYLKNKMDYSQESNVKMTHIINEFNDEMTQRIKNEEMKIERSKDELKREYLKEIE
                     KMGGRE"
     gene            3527..3757
                     /locus_tag="RJE42_002315"
     CDS             3527..3757
                     /locus_tag="RJE42_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877710.1"
                     /translation="MRQITAVIAPEHNRIHHDHKNKLKNDEELLINQMSSHFKKFKGE
                     FDNVAQGDWVKKAKNELDDISKKLKNIQRTEV"
     gene            4101..4292
                     /locus_tag="RJE42_002316"
     CDS             4101..4292
                     /locus_tag="RJE42_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877711.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(4431..4616)
                     /locus_tag="RJE42_002317"
     CDS             complement(4431..4616)
                     /locus_tag="RJE42_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877712.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYLNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(4552..4833)
                     /locus_tag="RJE42_002318"
     CDS             complement(4552..4833)
                     /locus_tag="RJE42_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV5877713.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            5122..5817
                     /locus_tag="RJE42_002319"
     CDS             5122..5817
                     /locus_tag="RJE42_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV5877714.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGVKVILAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT         2326 a          769 c         1093 g         1762 t
ORIGIN      
        1 aaattaacgc tcggtatatg tttggtatta ctcattatat tgattgtagg ttatgtcatt
       61 atgacaaaaa caaatggtcg aaacgctcaa attaaagaag catttaataa aacattaaat
      121 gtgtatccaa ccaagaatct tgaagacttt tacgataagg aaggttttcg cgatcaagaa
      181 tttgataaaa gagataaagg gacttggatt attaattctg gaatgaacat ccaattaaaa
      241 ggaggagcct taaaatccag agcgatggta ctttacatca atcgtaacac tagaacagct
      301 aagggatatt ttttaataag tgagacaaca gaagacaaaa aaggatacgt ccataataaa
      361 gataaaaaat accctgtcaa gatggaacat aatcaaatta ttccaacgaa gcccataagg
      421 gatgataagt taaaaaaaga gattgaaaac tttaagttct ttgtacaata tggggatttt
      481 aaagattata aagatgggga tatttcatac aatcctaacg taccaagtta ttccgcaaac
      541 tatcaattga ataatgatga ttataatgtc caacaattaa gaaaacgata tgatatatca
      601 accaaaaggg cgccggaatt aaaattgaga ggatctggcg atttaaaagg ctcatccgta
      661 ggttctaaag aacttgaatt taactttata agaaataaag aagaaaatgt ttattttacg
      721 gatagtatta atttcaaacc tactgaatag ggttaattga agtttggagt taaattttca
      781 taatgctcta attaggtaaa agataataaa gataattcca ggaattaatt gtaaatcata
      841 caaactttat cttttgattt aattgtttta taattcttat aagaaggttt gaaatacaaa
      901 atatttataa ttaaattcat ttgttaaaaa caatttttcg ttttagttgt ataaattaaa
      961 gattgagtca gtattaaaaa tataaggtgg agaaatgatg attcattcaa aaaagttaaa
     1021 attgtgttta tgcttaataa tactatctgt ttttataggg ggttgtggaa tgaaaaaaga
     1081 agaaagtagt aaagataaac aaattaaaga aaatttcaac aaaacattga gtttatatcc
     1141 aaccaaaaat cttgaagact tttatgataa agaaggcttt cgtgacgaag agtttgaaaa
     1201 aggagataaa ggcacttgga taattcactc taaaatgata attgagacaa atggtaggaa
     1261 tatggaatct agaggaatgg ttctctatat aaatcgtaat actagaacaa cgaaaggtaa
     1321 ttttgttatt agagaaataa ccgaggatag taaagggtat tcagacagta aagataaaaa
     1381 atatccggtg aaaatggaaa ataatagaat cgttccaatg aaaccaatac atgatgagaa
     1441 gttaagaaaa gaaattgaag actttaagtt ttttgtacaa tatggcaact ttaaagatat
     1501 taatgattat aaagatggtg acatttcata taatccgaat gtgccaagtt attcagcaaa
     1561 gtatcaatta agtaatgatg actacaatgt taagcaactt agaaagaggt atgatatacc
     1621 tactaagaaa gctcctaaac tattgttgaa gggcgatggc gatttaaaag gttcatcagt
     1681 aggatcaaaa aatctagaat ttacctttgt agaaaacaaa gaagaaaaca tctattttac
     1741 agattccgta caatatactc caagtgagga taacaattta tgagtcaaac tgaatatcaa
     1801 ataaaaccgg gcaatataaa agctaactct gaagaaacaa gtactgtatc taaaattagt
     1861 tatgaaatag aaaacgctaa taacagtggt ttaaaaaagg gtaaaatcaa tgaacaaata
     1921 gagggtctta aagaggaagg taaatttcct aaaaacttga aatacttaaa aagttatact
     1981 gatccaaaca ctggtacaac tacgactgcg ttcaaaaata aagacactgg caaagtaact
     2041 ttaggcatga ctggtacaaa tttacatttt gaagaaatgg gaaatgtctt gaaacatccg
     2101 tttaatcact ccgatcaaga tatgaaagat atgaaggaaa tgttgaaaga tttcggagca
     2161 gacgccaaca ttggtctagg tgctgtaacg gataaagatc cacattttaa agatacgcaa
     2221 gactttatta aagatatcaa aaaagaatat gaaattgaca caataactgg gcactcactt
     2281 ggcggtaggg atgcaattat tttaggcgtt agtaacgata tcaaaaatat tgttgtatat
     2341 aatcctgctc cattatcaat tacagatttt cgtataattg ctctgaataa tatgacgtac
     2401 agtgcgattg gcgacataga aattaaggaa atgttgaaaa actataacgg acatatagta
     2461 aggtttgttt ctgataaaga tgagttagat ggttttgtta gtcagtttat gtatgataca
     2521 gctggtgaga aaatagtttt gaacaatggc gaaggccatg atatgggagc ctttttaagg
     2581 aaatatgcac aagctaaaat tttagcagaa ttaagaaaag tcaaaggata cgaagacgca
     2641 aataataagt catttaaatc tgtaaaagac aatactaaat caaaattagg gaaagttgaa
     2701 gaacttagag tgaattggct tcaagcaaat ggtggagcat taacctcgtc tcaactaaaa
     2761 ttgttggaat ctgtttcagc attaataata gcagagggat taagtcaatt agttaaagag
     2821 gaaagtaacc aactggagaa gatgtacgat aatatgaagg acaaatttca agagaattgg
     2881 aagaattccc aagaagctgg aaataaaata ggaacaaaac tttcgcatga cggagtacta
     2941 acagcattaa gaaatggtga tgcgtatgaa agcaagtttg agactgaacc tttaaccaaa
     3001 atcgaacaaa agttaaaaga attgaatgac ataaatagtg attgtcacaa ctacgtctct
     3061 aaaattaaag atagtgtaaa tgcaattgta ggtaatgacc aattattagc gagtcagata
     3121 gcaggtgtaa tataatgttt actgaatata agaaattaag tgatttggaa actgcatatg
     3181 atgaagaaag aagaaaattg aacgataaat tagagcaact tcaggaaatt aagcatcaga
     3241 taaagttgga ttgtgaatat tcatacgatt gctttttata tctgaaaaac aaaatggatt
     3301 atagtcaaga atcaaacgtt aaaatgacgc atattataaa cgagtttaat gatgaaatga
     3361 cccagcgaat taaaaatgaa gagatgaaaa tagaacgttc taaagatgaa cttaaaagag
     3421 aatatttaaa agaaattgaa aaaatgggag gtagagagta aaggtgtggt atgaatcagt
     3481 tgttgaaagg ttaccaagcg ggccgagtat taatgctgtt ttgcaaatga gacagattac
     3541 tgcagttatt gcgcccgagc ataacagaat tcatcatgac cataaaaata aattgaaaaa
     3601 tgatgaagaa cttttaataa atcaaatgag tagtcatttc aaaaaattta aaggtgaatt
     3661 tgataatgta gctcaaggag attgggttaa aaaggcgaag aatgaactgg atgatattag
     3721 taagaaatta aaaaatattc aaagaacgga agtttaatat tctagccgat tcctgaagtt
     3781 aagacagcat gcgttcattc atgccattat gcaccgcaac aaaaaagctt ctaatgtgat
     3841 atgggaatct caaattccgt atcatgttag aagcttttaa tgtctaaaga acacctacat
     3901 tgtatcatat tttcttactt attaaacttt tatataatta actatttctt aattttccaa
     3961 aatagtgata aatttgtgaa atacatcaca aaccccttta tttatttgga aattcatgta
     4021 atattagact tgtaagaagt taataaatag agagacgaga gagtttatat aaatactata
     4081 taaacattgg agtgatgatt atgagaaaag agattgaagc gcttattttc tcagacgtaa
     4141 gtagctatga tatttacgtg aacactggtg taaatcaagg attaattggt gacatcaaag
     4201 atggttacct aactattgat tctatgcctt acattgatgc tgagcgtttg tatcactttg
     4261 ctatggaacg taaatcgtta gtcactaact agttcttatt gccaatgatt actaccccta
     4321 gtcggcggca attgaagtgt gattgatgta acttgccctc gttggtgagc aattgagggc
     4381 agaccccttt aattaagtaa accctaactc cccacaaatc tggaacgata ctaaaagcca
     4441 cgtcctatat tggatgtggc tttagtcatg cttatattat ttttaaaacg attacctaca
     4501 agatttaaat ataaaattct atcatgtctg ctatatataa acgaaatgat attagaatcc
     4561 ttcattttca acttgtttaa aattacgttt aatgccgtta taagaatata caacattata
     4621 taattttact tgggcgtcat ggacacttga gaagggcatt cttacatcgt ccatattttt
     4681 aacttcgtgc cgaaatgctt tttcagcttt ttgtctttct gcaagcgaca tacttagcca
     4741 ttcttctttg ctaaataaat cttcgaatcg aaaatcaaat gcggttcttt tactttctgc
     4801 tcttaatgct aaaacacgct catgatatgt catgttttat tcctccctct aatcattcaa
     4861 tcgaatgata aatcacagta ttaactttag tactaatata atgtttttac aataaagaag
     4921 tgacgatttt tcaacatttt taaaaataaa aaatataagt aacgattaat tgtatgtttc
     4981 gaatggcttg taatgtaatt tatctatgaa aatgaagcga gttattatga ccgttatatg
     5041 aattgtgata cgctttttaa aagtaatatg tatttatgat ttaccctgaa aaaataaaaa
     5101 gaatcaaagg agtcgatcat tatgacagta ttaacagata aagtagcagt agttacaggt
     5161 gcaggtagtg gtattggaga agcaatcgca acattactac atgaagaggg tgtgaaagtc
     5221 atcttagccg gtagaaataa agataaacta caaaatgtgg cgaatcaatt agctcaagat
     5281 agtgtgaagg tagtgccaac agatgtaacg aaaaaagaag aagtcgatga attgataaaa
     5341 attgcacaac aaacattcgg tggtttggat attgtcatca atagtgcggg gcaaatgttg
     5401 tcgtctaaga ttactgatta tcaagtagat gagtgggata gcatgattga tgtgaatatc
     5461 aaaggcactt tatacacggc acaggctgca ttaccaacta tgttagaaca atcaagtggc
     5521 catcttatta acattgcatc tatttctggc tttgaagtaa cgaaaagcag tacgatttat
     5581 agtgcgacga aagcagcagt tcacactatt actcaaggat tagaaaaaga gttggcaaag
     5641 acaggcgtta aagtaacaag catttcacca ggaatggtag atacagccat aactgccgca
     5701 tacaatccaa cagaccgtaa aaaacttgaa ccacaagata ttgcagaagc agtattatat
     5761 gcattaacac aaccaaagca tgttaacgtg aatgaaatta cagtgcgtcc tgtataaaaa
     5821 tgtgtttgag gtgtgggaga tagacattta atgtaatagc tataaatatg aattattgtg
     5881 cgattgaaat gatatataga tactttaaat caatagaatt agtgtgttct tttatttaac
     5941 aattatataa
//
LOCUS       DAPIDM010000146         5823 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000146 DAPIDM010000000
VERSION     DAPIDM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.146"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..139)
                     /locus_tag="RJE42_002320"
     CDS             complement(<1..139)
                     /locus_tag="RJE42_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyrate permease"
                     /protein_id="HDV5877715.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TY"
     gene            487..1608
                     /locus_tag="RJE42_002321"
     CDS             487..1608
                     /locus_tag="RJE42_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDV5877716.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            1657..2925
                     /gene="icd"
                     /locus_tag="RJE42_002322"
     CDS             1657..2925
                     /gene="icd"
                     /locus_tag="RJE42_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV5877717.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVEKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVEKEGRDAANVAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            3315..4016
                     /locus_tag="RJE42_002323"
     CDS             3315..4016
                     /locus_tag="RJE42_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5877718.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKE"
     gene            4019..5680
                     /locus_tag="RJE42_002324"
     CDS             4019..5680
                     /locus_tag="RJE42_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDV5877719.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDKKVFSSGKLKPIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKMQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNITHNIEQIGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2097 a          776 c         1122 g         1828 t
ORIGIN      
        1 tatatgttag taatatagaa gggccagtta atgcaatcgt ttgaccagcg cctaagaata
       61 gccctgtacc aattgcgcca ccaatagcaa ttaattgaat gtggcgattg ctcagttccc
      121 tttgtaattt ttcagccata atacatctcc cttaaatata gatatgttta ttatgcactt
      181 atattaggat atatacaatt attttcggta aaaatgtgta aaattccatg ttaatatact
      241 ttggatttta taatcatata taataaccaa ttgaaaattt tattctatgc taaaagttgg
      301 tgatgaatat aatcttgaaa aagctttaat ggtgctattt gtggccattc tgtgacattt
      361 acatagattt acaaaataat tgttgcacat ataatgccag tttttatatt tcacaaacga
      421 aatgcgttta gctataatat tagttgaaag ccatttcata aagaaacagt aaaggggaaa
      481 tttatcatgg cagaattaca aagaggttta gaaggggtta tcgcagcgga gactaaaata
      541 agttcaatta ttgaaagtca attgacttat gccggctatg atattgatga tctagctgaa
      601 aatgcgcaat ttgaagaagt tattttccta ttatggaact atagattgcc aaatgaagaa
      661 gagcttgctc atttaaaggg gaaattaaat caatacatga cattaaatcc tcgtgtgtat
      721 acacattttg aggagtatgt tacagatcac gtgcatccaa tgacagcatt acgtacgtca
      781 ttatcatata ttgcacattt cgatcctgat gctgaaaatg aatcagatga aaatcgttat
      841 gaaagagcaa tgcgtataca ggctaaagta gcatcattag ttacagcgtt tgctcgagta
      901 agacaagata aagaaccact taagcctaat cctgacttaa gttatgcagc aaacttccta
      961 tatatgttac gtggggaatt accaacagat atagaagtgg aagccttcaa taaagcgctt
     1021 attttacacg ctgatcatga gttgaacgca tctgcattta cagcacgttg tgcggtatct
     1081 tcattgtcag atatgtactc aggtattgta gcagctgtag gttctctgaa agggccatta
     1141 catggtggtg caaatgaaca agttatgacg atgttatctg agattgggtc aattgaaaat
     1201 gttgatgctt acttagatga aaaatttgct aataaagata aagtaatggg cttcggtcat
     1261 cgtgtatata aagatggtga tcctagagcg aaatatttaa gagaaatgag ccgtcaaatt
     1321 acgaaagacg ctggtcgtga agaattattt gaaatgtcag tgaaaatgga aaaacgcatg
     1381 gcagaagaaa aaggattaat tccaaatgtt gatttttata gtgcgagtgt ttatcactgt
     1441 atggaaatac ctcatgactt attcacgcca atctttgctg taagtcgttc tgcaggatgg
     1501 attgctcata ttttagaaca atataaagat aatagaatta tgcgtcctag agcaaaatat
     1561 attggcgaaa cgaatcgtaa gtatattccg cttgaagaaa gaaaataatc aatacaaatt
     1621 aaaaatgaag atgtaaaatt tggaggtaaa ataactatga ctgcagaaaa aattactcaa
     1681 ggaactgaag gattaaacgt acctaatgaa ccaattatac catttattat cggtgatgga
     1741 attggaccgg atatttggaa ggcagcaagc cgagttatag atgctgctgt tgagaaagct
     1801 tataatggcg aaaaacgcat tgaatggaaa gaagtgctag ctggccaaaa agcatttgat
     1861 acaactggcg aatggttacc tcaagaaaca cttgatacaa ttaaagaata tttaattgct
     1921 gttaaaggac ctttaacaac accaattggt ggtggtatta gatcattaaa tgtagcttta
     1981 cgccaagaat tagatttatt tacttgttta agaccggtac gttggtttaa aggagtacca
     2041 tcacctgtta aacgtccaca agatgttgat atggttattt tccgtgaaaa tactgaagac
     2101 atttatgctg gtattgaatt taaagaaggt acaacagaag ttgaaaaggt aattgatttc
     2161 ttacaaaacg aaatgggtgc gacaaatatt cgattcccag aaacttcagg tattggtatt
     2221 aagccagttt ctaaagaagg tactgagcga ttagttagag cagcaattca atatgctatc
     2281 gataataacc gtaaatcagt tactttagtt cataaaggta atattatgaa atttacagaa
     2341 ggctcattta agcagtgggg ttacgattta gcattaactg aatttggtga tcaagtattt
     2401 acttggcaac aatatgacga aattgttgaa aaagaaggca gagatgctgc taatgttgca
     2461 caagaaaaag ctgaaaaaga aggcaagatt atcattaaag attctattgc tgacattttc
     2521 ttacaacaaa ttttaactcg tccagctgag catgatgttg tagcaactat gaacttgaat
     2581 ggtgactata tttcagatgc attagctgca caagttggtg gtattggtat tgcgccaggt
     2641 gcaaacatta attatgaaac aggtcatgct atttttgaag caacacatgg tacagctcca
     2701 aaatatgcag gtttaaataa agtgaatcca tcttcagtaa ttttaagttc tgtattaatg
     2761 ttagaacatt taggatggca agaagcggca gataagatta cagattcaat tgaagataca
     2821 attgcttcaa aagttgttac ttatgacttt gcccgtttaa tggatggtgc tgaagaagtt
     2881 tctacatcag catttgcaga tgaattgatt aaaaatttaa aataagcaaa ataaacttag
     2941 gtgagtctag caactttaat tgctaggttc acctttttaa atgaattgaa gttggcagca
     3001 ggatagaaat gataaagagt cacgaatgat tgattatgta gtgtttctta cacattaact
     3061 aaagcgaatg tgtatataag cttaggaata aatgagtaaa actcatgcat aggtagtggg
     3121 atagaaatga tattttcaca aaatttattt cgttgtccca gagtaagtca tgtaaattat
     3181 agaaggaaat ataagaagta ttttatttat gtgtaaagta atgtaaaaaa tgtgtaaaga
     3241 taatgtgtag gatgttattt ttataaatag ttcggttata atgtaaatat aattataagt
     3301 tagggaggca taccatgtcg caaaaagtgt tggtagtaga tgacgaacat tcaatcgtaa
     3361 cattacttaa atataactta gaaacagctg gttatgaagt tgttgttgca tttgatggtg
     3421 atgaggcttt agagaaggta gaaaatgaac agccagattt aattatttta gatgttatgc
     3481 tacctaaaaa agatggcatt gacgtatgta agactgtaag aacaaataaa aatttagtcc
     3541 ctatattaat gttaacggcg aaaaatgatg aatttgatcg ggtattaggt ttagaattag
     3601 gtgccgatga ctatatgact aagccttttt cacctagaga agttgttgcc cgtgtgaaag
     3661 ccattttaag acgttctcaa tttgtaaaag aagtagaaga gattgacgat gatattacta
     3721 tcggatcgat acgtattaga cccgattatt ttgaagtata taagcataat gagctattgg
     3781 agctaacacc gaaagaattt gaactgttgc tttatttgat agaaagacaa ggcagagtca
     3841 ttacgagaga gcatatgtta aatacggtat ggaattatga atttgcagga gactcaagaa
     3901 ttgttgatgt acatatcagt catttaagag ataaattaga agataatcct aagaagccac
     3961 aacttattaa aactgtgcgt ggcttaggct ataaattgga aagacctaaa gaataatgat
     4021 gaagtttcac caccgcttaa tgttattgat aagcagtata ttaattatca gttttgttac
     4081 attaggcatt attattcacg cttccatcac atatgtttta tcagaaaaac acgaacaagc
     4141 tctagcaaca gatgcaagat catatattta tttagtccaa gataatgaga ttgataaagt
     4201 taaagaaatt gtaaaacagc aaaatataga tttattgata actaaaaatg ataaaaaggt
     4261 gtttagtagt ggcaaactga aacctatccc aaatagtgat agctatattc aaaaaaggga
     4321 tgtttttatt tttaataaga aggttaacgg ttatcatgta tggattaaag gttacaataa
     4381 cgatattaca gaaatgcatt ggacgttgtg gaaatatctt atattgacat gtttagttgt
     4441 acttatctgt ctgtattttg caagccgtag ttttaagcgt acattaatcc gtcccataca
     4501 agaagttact tatgcaacac aattgttggc aaatggttat tatcacatac gtgtacctga
     4561 aagtaatgtt gtagaaacta aagcgctttt tgtgtctacc aatgatctag cacgacgcct
     4621 tcaaaagctt aataatgagc aaaaaatgca atccaatcgt ttaaaaacaa cgattgaaaa
     4681 tattccaagt gctattttaa tgatagatcg aaatggaaaa attgttgttg caaataaagc
     4741 gtattatgaa cagttcaaca tcacacataa tatagaacaa ataggctatc acggctatgt
     4801 taatactgaa attgagcaat taattttaga aagttttaaa gttgaaaaac ctatttatga
     4861 acaattggaa gttgcaataa accaagtcca tgccaagtac tttgatattt cttgtgttcc
     4921 tattttaact agatctcaaa aaagtttgca agggatttta gttgtgatgc atgacataac
     4981 aaatctgaaa caacttgaaa atctaagacg tgaatttgta gcaaatgttt cacatgaatt
     5041 aaaaacacct attacttcaa ttaaaggatt tgctgaaaca cttattgatg gtgcaaaaaa
     5101 tgatgcggaa tcattagaca tgtttttgaa cataatttta aaagaatcaa atcgaataga
     5161 atctttagta acagatctgt tagatttatc gcatattgag caacataccg agttagatac
     5221 agactatatg aatttatcag atttaacgcg gcgtattatt gataatatga tgactcaagc
     5281 caatcaaaaa aatatttcca ttcatactga tattgaaaag gatgtcattg ttaaagcaca
     5341 agaaagtaaa attgctcaag ttatcacgaa tttgttaacg aatgcaatta actattctta
     5401 tgaagatgga gatattaacg ttcgtgtgta tcgagatgac tttcgtgtca ttttcgaagt
     5461 acaagatttt ggtataggta ttaaattaga agaccaacaa cgtatttttg aaagatttta
     5521 tagagtagat aaagcacgaa gtagagattc tggtgggaca ggtcttggat tatcaattac
     5581 aaagcatata gtagaggccc accaaggtaa tattgaagtg aatagtcaag ttggcaaagg
     5641 gtcaacgttc aaagttattc taaaagatta taaagaataa aaattaaagt ggtaacagcg
     5701 cgtgtatttt tcacgaagtt gttgctgctt ttttatttcg ttcaaatctc tattggtatt
     5761 aaattagagt taatgaagtg gaatatattc ggttgtcaat tttgtcattt gcatatgtcg
     5821 ttt
//
LOCUS       DAPIDM010000147         5716 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000147 DAPIDM010000000
VERSION     DAPIDM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.147"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(114..1466)
                     /gene="tet(38)"
                     /locus_tag="RJE42_002325"
     CDS             complement(114..1466)
                     /gene="tet(38)"
                     /locus_tag="RJE42_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDV5877720.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(1473..2180)
                     /gene="deoD"
                     /locus_tag="RJE42_002326"
     CDS             complement(1473..2180)
                     /gene="deoD"
                     /locus_tag="RJE42_002326"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV5877721.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2416..3171
                     /locus_tag="RJE42_002327"
     CDS             2416..3171
                     /locus_tag="RJE42_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5877722.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNPSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(3362..4087)
                     /gene="sasD"
                     /locus_tag="RJE42_002328"
     CDS             complement(3362..4087)
                     /gene="sasD"
                     /locus_tag="RJE42_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDV5877723.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMLQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(4456..5055)
                     /locus_tag="RJE42_002329"
     CDS             complement(4456..5055)
                     /locus_tag="RJE42_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDV5877724.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            complement(5323..>5716)
                     /locus_tag="RJE42_002330"
     CDS             complement(5323..>5716)
                     /locus_tag="RJE42_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDV5877725.1"
                     /translation="LIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITE
                     FFLLIWRFIDITKIKVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIG
                     IVVYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         1904 a         1001 c          861 g         1950 t
ORIGIN      
        1 taaagtgtga tctatatatt tctcaaattt catacttact actcctcgtg ttatataatc
       61 tctttattta attttactat aaatacgaat atatctcgcg aatttatatc tatttatttt
      121 tcagattgtg tccaacgatt taagatgacg aataaaacta atccgacaat actcattaaa
      181 cctaaaataa ttaaaataat accgaaatgt gaacttaatg catcattgcc tgggaaattt
      241 aatgctttaa aatcgattag agccgcagca atcgcaatac ctacagatac cgccacatta
      301 ataattaaat tataaaaacc aatagccaca ccagtcatat taagatctat tgttttaatg
      361 gcttcgttaa gtaaaggtgc atacattaaa gcaaagctac ctgcaaagaa tatcatagaa
      421 atgacgaaga ttgaaatgtg attacctact gcaaatgcag gtaaaatcaa gctcagtgcg
      481 attaaaataa ttgctgtgat aatcgcttgt tttgaattaa gatattcgcc gattttacca
      541 cttagtgcac caacgatgac tgctacaata taacccggta ctaatagcag tgatgttgtg
      601 tctagctgaa gatgataaat ttgttccatt atgaatggga acgtaaaaat ataacccaat
      661 tggatggcat acattacaaa tactataaat aaaaatgaag cataacgttt attttggaaa
      721 aatgatttgt ttactaatgg acgttgcgca tttttaatat atagcgcaaa aacgataatc
      781 gcaattaaag caccaatcat atataaccaa ttaaagttcg taataaacag catgactgtt
      841 gtagcgatag ttgccactag aattaagcca acaaaatcta aatgagcttt atttgtatta
      901 ttttcttttg gtaataattt aaataggaat ggcaacgtaa atactacgat taatgcaatt
      961 aaaaacatat ttgtccagtg taaatacgta gaaataaatc cacctgataa tgtaccgata
     1021 actaatgaca aggaataact gctcgtactt aagccaaggt aagtcttctg gtcctcttta
     1081 gaaagatact ttgcaacata tatcacatat aatgtctctg cagcagctaa acctgcagtt
     1141 tgaataatac gtccaactaa aagtaatgcg aattgatgtt ggaaaatgta accaataatt
     1201 gatccgataa tgattaatat cacgccataa ataaatagtg tgcgtatact aatggcatca
     1261 gataatgaag cgtatactac agcgccaata ccaattacta aaccggctaa tgtcgcttgc
     1321 caacttactg tcgttacaga aatgtttaag tcatcagcaa tggctacaga aatcaattta
     1381 aatgagttgt ctataaccaa actgaataca aataagaata ataaaatagg tactgctttc
     1441 tttattttag aatattcaac attcataatc atctacacca atgacagtgc aatttcaatc
     1501 atatctgtaa atgcacgttc cctttcctca ggtgttgttg acgtttcatg aattaaatga
     1561 tcgctcactg tgaacacacc taatgcttcg acaccagcgt aaattgcatt catgtataat
     1621 gcagctgatt ccatttctac acctaaaata cccatacgca tccaacgttc actcgctgtt
     1681 gtatccgcgt tatagaaaat atcacttgat aacacattac ctacatgatg acgtacacct
     1741 ttgtcacgtg ctgtttcaac agctttttct aataactgat aagaagcaat tggcgcaaaa
     1801 tgacctggta attgatattg ttgaacgtaa tttgaatcag tagaggcacc ttgtgcaata
     1861 atcacatcat ataaatcaat gttttcttgc atcgcgccac aagaaccaac gcgaattaat
     1921 tttttacaac caaatgtatg aattaattca taagagtaaa tgccaataga tggcataccc
     1981 attcctgaac ccatgacaga aactttttta cctttatatg ttccggtaaa accaaacata
     2041 tttcgcactg tattgaactg ttccacatcg tccaaatatg tttctgcaat gaacttagct
     2101 cttaacggat ctcctggcaa tagaaccgtt tctgcaattt cgacgtcatt cattggttta
     2161 atgtgtggtg ttgatttcat aaaatattcc tcccaatatt tgtatagtaa tttgtctatt
     2221 tgtctaacca tgtatataat aatatttttt agaagcgctt tcaagtatta cattttgaca
     2281 atattaagaa cattttacag ttatctctac tgatcttgca taaaaccaat gttcaagttg
     2341 tttctatgtt taatttttat aatttttcca attcgagtaa aaattattgt atgatgagag
     2401 tgattaagta actttatgga ggttgtctac gtgacgcaaa cacaaccaaa atttttaacg
     2461 atttataata cgttatataa agaaattcaa ataggaaaat atccgagtgg ccatgcatta
     2521 cctactgaaa aggaactttg tgcgcgcttt gatgtcagtc gtatgacttt gcgccaagct
     2581 ataaaattgt tagccgaaga tggcatcgta gagagtacaa gagggaaggg acactttgtt
     2641 attccacaaa caaatgccca acacgcttca agtatggatc aatttaagca tccacttgat
     2701 caaatattgc tagctcgtgt atctatgtca tcaattaatt atcgtgttga tttagaaagt
     2761 gaatatacaa atcatttgtt tgcaaaccat ccttctgccg tcattgcaat ggaacgctat
     2821 tatcaaaaaa aggacaatcc ttctaagcaa gctgatgcat tttgttttac ctttataccg
     2881 ttaaatgtca ttgacacatt taaagtgaat acacaaaacg aagacgacat gaagacgttc
     2941 gttgagcaga cgatttatag caatgcatat caatcagatt taaaaatgtc tattaccaaa
     3001 gcgccccact tcaaaaatca tagtcacgtg tttgatggtg atacacattg ttggctgatt
     3061 attgaaacat tgtacgcaca aacgccctat ccgattatga ttaataagtg gtatattcct
     3121 caggaaatat ctgaattaac actgacacgt ataagacaat cggattatta aaattattaa
     3181 aatataagaa attttgtgat gactacttag gaagtgggca agaaatggaa aagcttgtta
     3241 caagcgcaat ttcattcagt caactactgc caatataaca ttagcgagcc taggacattg
     3301 atttatgtcc taggctcgct cagataatca cacgattttt ctattattaa tcgtattatg
     3361 tttatttgtt tgttgaacgt cgtctaaatg ctaagaataa cccagatact aatagtagtg
     3421 cggcaatact tgcagtaaga atacttgatg tcatactttc accagcagct ggtaaaactt
     3481 ttttgttgct ttggttcatt tgatgatttg atgaatgcat atttgctttt ggactcatca
     3541 tttgatggtt tgatgattgc atatgacctt gtagcatgtg tggctgttgg ttcatgtgtg
     3601 tgttctgatt catatggcct tgttgactca tatgtcctgt tttattttgc accatttcac
     3661 taccaccatg attaacacca tttgcatcta cattcgtttg ataaaaattg ccatcttttg
     3721 ttaaaggtaa cgcatggcca ttcatatctc taacagcatg tggtttaaat acccctacat
     3781 tagttgtaat tatcacattg tatttatcag caatattttg cgtcgcatca tacaatttca
     3841 tattatagtc ttgcatatta ctgccagcgc ttaattgggg taaagctttc tctaattcag
     3901 tatattgtgc ttgcgtcatt ccttgtggta ctactccgcc ataagggcga tgattttgta
     3961 cattttggct ttgtttattc gacacattca ttgaagttgt gtcagcatgc gcattttgaa
     4021 aaggcatact tgagaatact aaagtgcttg ttacaattaa agaacctact gttgctaatt
     4081 ttttcattgt cttttcccct tatattacaa tttgattaca tttatattat catatgatta
     4141 caaaagaaat gcaacaaaat ttttgaatca ttacaatttt ttataaaaat ttcactttag
     4201 attcacaata attacttatt ttgtcaattt atttaatgac aatatgttga ttaatcaata
     4261 gtgttgtctg atgtatatag catttaggtc atcgttatag tcaacaataa taaggtattt
     4321 cgagttgaaa tttatcttat tattttgcca cttttacgcg ctatcccatt acacaaaaac
     4381 gaataagtaa agatattaag tacaaaaaag gtgaaacacc ttgtagatgc tccacctcaa
     4441 ttatattaag ttatattatt ttgctgcttg gtataattca tcaacttttt tccagttaac
     4501 aatattccaa aatgcagtca tatagtctgg acgtttattt tgatatttca gatagtaggc
     4561 atgctcccaa acatcaaata gtaagattgg tgttttgcct tctgttaatg gattatcttg
     4621 gtttggcgtt gtcacaattt ctaattttcc atcattaaca actaaccaag tccaacctga
     4681 tccaaataat gttgttgctt tattggcaaa ttcattttta aattcatcta aagtgcccca
     4741 ctgcgcttta atgtcgtcta ttacgccacc tttttcttca gaattaggtg atagtatttc
     4801 ccagaataat gaatggttaa aatgaccacc gccattatta cggactgaca tcctcatcgc
     4861 ttccggtacc ttgtctaagt tagcaatcat atccgctagt gattgatgct ctaactctgt
     4921 tccttcaact gttgcgttta atttcgtcac gtacgtattg tgatgtttgt cgtgatgaaa
     4981 ctccattgtt ctttgatcta tatatggttc caatgcatca tatgcatatg gtaaatttgg
     5041 taatttaaat gccataagta tattcctcct tttatgaata tacttttata ataattaatt
     5101 tcgggtgtgt tttgcaataa ttattattta tttgttatat ccaattatat aaaatttaaa
     5161 tattcgaaat tgatataata tttaatattg aatgcaaaaa ggcatttaac tccttttgtt
     5221 tcccgctttt aaaaagaatt aaaaaagaca acttccattt ttcaataaga aattgccttt
     5281 tcttcttcat cttaaatttc taaagtatag cgttaccgat acttaaattg ttttatgtcg
     5341 aagatgcctc aatatttgcc atacgtattg atttttcata gtcatcatta ataaaagata
     5401 gactactata ccaatcgcaa ttaatagcag cgtagcgtac attgtaggag gcaaataatg
     5461 attgaccaca ccaagcacaa taaacatcat aacggcagca atgacacatt gaatcgtact
     5521 tacaatattc aacttcactt tgattttagt aatatcaata aatcgccaaa tgagcaagaa
     5581 aaactctgta attaaacgcg caatagcagc accgtaaatt ccataaaaat atatcaaaac
     5641 aagacataat actaaattca ttaccgcacc aatagtaatt gacgcattat ataatcttat
     5701 tttattcact attaat
//
LOCUS       DAPIDM010000148         5689 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000148 DAPIDM010000000
VERSION     DAPIDM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.148"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            91..237
                     /locus_tag="RJE42_002331"
     CDS             91..237
                     /locus_tag="RJE42_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877726.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            227..751
                     /locus_tag="RJE42_002332"
     CDS             227..751
                     /locus_tag="RJE42_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDV5877727.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      738..835
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            910..2001
                     /gene="gcvT"
                     /locus_tag="RJE42_002333"
     CDS             910..2001
                     /gene="gcvT"
                     /locus_tag="RJE42_002333"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV5877728.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2021..3367
                     /gene="gcvPA"
                     /locus_tag="RJE42_002334"
     CDS             2021..3367
                     /gene="gcvPA"
                     /locus_tag="RJE42_002334"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDV5877729.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AESEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3360..4832
                     /gene="gcvPB"
                     /locus_tag="RJE42_002335"
     CDS             3360..4832
                     /gene="gcvPB"
                     /locus_tag="RJE42_002335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDV5877730.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPISDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEYFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5196..5582)
                     /locus_tag="RJE42_002336"
     CDS             complement(5196..5582)
                     /locus_tag="RJE42_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV5877731.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
BASE COUNT         2033 a          766 c         1056 g         1834 t
ORIGIN      
        1 agaatttaaa tgagtactat taaaaaggat gtgaaataat ttattaattt tgatttatat
       61 aaagaaaaag gatatgttac tccgttctta ttgatgctaa cagcaattta tttttccatt
      121 ttcacttttt atatcagtca atatagttta aaattaaaga cattatataa tattgaaact
      181 gactataact ataagattgt tttgttattg aaagaaggga aaacgcatga atcatgataa
      241 atcaccaata attttaattg gtttcatggg taccggtaaa tctacgattg gtaaatacgt
      301 tgcagatgag caaaatttat catttattga tatagattca tatatcgaag aaaagtataa
      361 gttaacaata ccagaaatat ttagtaaaca tggtgaacaa tatttcagga atttagagtt
      421 cacatgtttg caagaatgta ttaacactgc agatataatt gctactggtg gtgggattat
      481 tgaaagtgaa gaggcattta attttttgaa aaatcaaaaa aacattattt ggttagattg
      541 taatattgat attatatata gtcgaatcaa tgatgaccca catcgaccta atgcaaataa
      601 taagacaatc aagcagttaa atgacttgta ttgctcgcgg attttaagat ataatgaaat
      661 cgcattcaag aaatttgata gtcatttgct atcaatttca gaaatatatt atgaattgct
      721 aaatttaata aaagcgagtg atcagtatta gagagaatag agcgttaaga ctctatcgcc
      781 gaaggtgcaa gtaatttatt acgaaactct caggcaaaag gataatactg taacgcgttc
      841 ctgaattggt gatttataaa cagggtagcg attgctatcc tgtttttata attttaaggg
      901 ggtatttcaa tgtcaagtga tttaaaacaa acacctttat atcaaaatta tgttgataga
      961 ggtgcaaaaa ttgtggaatt cggaggatgg gcgatgcctg ttcaattttc aagtattaaa
     1021 gaggagcata atgctgttcg atacgaaatt ggcctgtttg atgttagtca tatgggtgaa
     1081 attgaagtaa caggtaaaga tgctagtcag tttgtgcaat atttattatc aaatgatact
     1141 gataatttaa ctacttcaaa agcattatat actgctttat gtaatgaaga aggcggtatt
     1201 attgatgatt tagtaatata taaattagct gacgacaatt atttattagt cgttaatgct
     1261 gctaatactg aaaaagattt taattggatt ttaaaacaca aagagaaatt tgatgttgaa
     1321 gtacaaaatg tatcaaacca atatggtcaa ttagcaatac aaggaccaaa agctagagat
     1381 ttaattaatc aattagttga tgaagatgta actgaaatga aaatgtttga atttaaacag
     1441 ggtgtcaaat tatttggagc aaatgtcatt ttatctcagt caggttacac aggtgaagat
     1501 gggtttgaaa tttattgtaa tatcgatgat actgaaaaaa tttgggatgg tttattagag
     1561 tataatgtta tgccatgtgg gttaggcgct cgtgatacat taagattaga ggctggattg
     1621 ccattacatg gacaagattt aactgaatca attacaccat atgaaggtgg tatcgctttt
     1681 gcaagtaaac cattaattga tgctgatttt attggtaaat ctgtattaaa agatcaaaaa
     1741 gaaaatggtg caccaagaag aacagtggga ttagaattac ttgaaaaagg aattgcaaga
     1801 actggttatg aagttatgga tttagatgga aatattattg gagaagtaac ttcaggaaca
     1861 cagtctccat catcaggaaa atcaattgca cttgcaatga taaaaagaga tgagtttgaa
     1921 atgggtagag agttgcttgt tcaagttcgt aagcgtcaat taaaagcgaa aattgttaag
     1981 aaaaatcaaa ttgataaata attaaaaagg ggtgtgcatt gtgagtcatc gttatatacc
     2041 tttaactgaa aaagacaagc aagaaatgtt acaaacaatt ggtgcaaaat ctataggaga
     2101 attattcggt gatgtaccaa gtgacatttt attaaataga gatttaaata ttgctgaaag
     2161 cgaagcggaa acaacgttac ttagaagatt aaatcgcatt gcaagcaaga atatcactaa
     2221 agaaacgcat acatcatttt tgggtgctgg tgtttatgat cattacgcgc catcagttgt
     2281 ggatgctatg atttcaagat cagaatttta cacagcgtat acgccatatc aaccggaaat
     2341 ttctcaaggt gaattacaag ccatttttga gttccaaact ttaatttgtg aattaacaga
     2401 tatggatgta gcaaattctt ctatgtatga tgggatgact agctttgctg aagcatgtat
     2461 tttagcattt agtcaaacaa agaaaaataa aattgtagtc tcaaaaggtt tgcattatca
     2521 agcattacaa gtacttcata cttatgctaa gacgcgtaag gaattcgaag tagtggaaat
     2581 cgatttagat ggcactgtta cagatttgaa aaaattagaa gcagcagtgg atgatgaaac
     2641 agcagctgtt gcagtacaat atccaaactt ctacggttca attgaagatc ttgaaaagat
     2701 tcatagcttt attgaagata aaaaagcatt atttatcgta tatgcaaatc cattagcatt
     2761 gggattactt acacctccag gttcatttgg tgcagatatt gttgtaggtg atactcagcc
     2821 attcggaatt cctgctcaat tcggtgggcc gcattgtgga tattttgcta cgacgaaaaa
     2881 gttaatgcgt aaagtgccag gtagattagt cggtcaaaca caagatgatg aaggtaatcg
     2941 tggctttgta ttaacactac aagcacgtga acaacatatt cgacgcgata aagcgacatc
     3001 taatatttgt tctaaccaag cattaaatgc cctagcgtca tcaattgcaa tgtcagcact
     3061 tggtaaacaa ggtatctatg atattgcagt tcaaaatata gaacatgcta actatgctaa
     3121 acaacaattt attaaaaaag gttttgaagt attagatggt acatctttca atgagtttgt
     3181 agtcaaattt gacaaaccaa ttcaacaagt taacgaggaa ttagtgaagt acaatattat
     3241 cggtggtttt gatttaggtg ttgtttcaga cgactttaaa aatcacatgt taatagctgt
     3301 aactgaatta agaacaaagg atgaaatcga tacatttgta gaaaaggctg gtgagttaaa
     3361 tgactagtaa atcaagtcca ttaatttttg aaagatctcg tgaaggcaga tatgcatatt
     3421 cattaccaat aagtgatatt aaaacaaatt ctgttgagtc attgttagat gataaattta
     3481 ttcgtaaaaa caaagcagag tttcctgaag ttgctgaact agacttagta cgtcattata
     3541 ctgaactttc aaataaaaat ttcggtgtag ataacggatt ttatccattg ggttcatgta
     3601 cgatgaaata taaccctaaa attaatgaaa aggttgctag aataccagga ttcagtgaat
     3661 cacatccatt acaagatgaa gaccaagttc aaggttcatt agaaattatt tatagtttgc
     3721 aagaagaatt aaaagaaatt actggtatgg atgaggtaac attacaacct gctgctggtg
     3781 cacatggtga atggactgca ttgatgatat ttaaagctta tcatgagaat aatggtgaag
     3841 gtcatcgtga tgaagtcatt gtgccagatt ctgcgcatgg tacgaatcca gcttcagctt
     3901 catttgcagg atttaaatca gttactgtaa aatcaaacga acgtggcgaa gttgatattg
     3961 atgacttgaa acgtgttgta aatgaaaata cagcagctat tatgttaact aatccaaaca
     4021 ctttaggtat tttcgaaaaa aacattatgg aaatccgtga aatcgtccat aatgctggtg
     4081 gtctattata ttatgatggt gcgaatttaa acgctattat ggacaaagtt cgcccaggag
     4141 atatgggatt tgatgctgtt catttaaact tgcataaaac ctttactggt ccacatggtg
     4201 gcggcggtcc tggttcaggt ccagtcggtg tagtaaaaga actagcaagt tacttaccaa
     4261 agccaatggt tattaaagat ggcgacaaat ttaaatatga taatgatatt aaaaattcta
     4321 tcggacgtgt aaaaccattt tatggtaact ttggtattta cttaagagct tatacgtata
     4381 ttcgaactat gggagcaact ggacttaaag aggtttctga agcagcggtt cttaatgcga
     4441 attatattaa agcacgttta tctgaatact ttgaaatacc ttataaacaa tattgtaaac
     4501 atgagtttgt gttaagtggt gtgcgtcaaa aagaatttgg tgtacgtact ttagacatgg
     4561 ctaagcgact attagatttc ggtgtacatc caccaacaat ttacttccca ttaaatgttg
     4621 aagaaggtat gatgattgaa ccaactgaga cagagtctaa agaaacgctt gattatttta
     4681 tcgatacatt aattagtatt gctgaagaag ctaaaaacga tcctgataaa gtgctagaag
     4741 caccacatac aactgtgatt gatcgattag acgaagctac agctgctcgt aaaccaatat
     4801 taaaatttga aaatcttaaa caggaaaaat aaggtattaa acacatattc ctggaattat
     4861 tattctaact ttgtatgaag atttaaggat aatggtttca aaatcaatta aaaaagacaa
     4921 tttctatttg aacaagaaaa ctaaaccgaa gtaataactc ttagggtttg gtattattct
     4981 ttcatagaaa ttgtcttttc actttttaga ttgcggtaat tgaatcgtat tgaaaatgag
     5041 ttgaagtttc tttattatgc tgaaactaag tttaatgatc tgagaagcta gagaaatata
     5101 gtaacagact ttgttaaaaa attaaaatct gagtattgtt attgatgatc aattaacact
     5161 cagattttat taaatcgtat attaaattta caaaactatt ttttagactt tatttttcca
     5221 gtccattttt tatagccgcc ttttaacata tagatatctg tatatccatt ctttttcaaa
     5281 atacgagcgg ctctatagct agcaatccca ttggcatcac ataagtatac cggttggtct
     5341 tttcttaatc cttggaatcg ttgcctgaac attgtcatag gaatattgcg agacccatta
     5401 atgtgaccgt agtcatagtc aactttctct ctaacatcga tgacttgagc ttttctaatc
     5461 ccattatgga attcattttg atctaattct ttaacagctc gcttgttaag aatttgttga
     5521 acaatcatat aagcaataat tgctataact aaaattattg cgatgtacaa actagcactc
     5581 atcttgcatc ctccctaatt aaacgataat ataattataa gactgttagg tcaaattatc
     5641 aaaatatttc actaaagaat tgcaattatt attcatttag ttaaattga
//
LOCUS       DAPIDM010000149         5684 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000149 DAPIDM010000000
VERSION     DAPIDM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.149"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..711
                     /locus_tag="RJE42_002337"
     CDS             <1..711
                     /locus_tag="RJE42_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877732.1"
                     /translation="QDAQKKVLYEDIFIEENKDNLKFNRINEICSNDLNVLGYIISYG
                     LTESEAFSAENVLINFLNLTNKTTLTNMINGHGSKAYLVEDLENEFGYDSINLENINT
                     NELILAVKIRDAFLLDKDESKEYPINESKRDRNNLKSRTLGSWIIGKDKIHKIKYIIG
                     INTGANNAVVSAYEVSFEQAESIETNNGRMRYAFIGLSERDATLKKLNLYKKALPDLR
                     FGSGSATAYINNGTMKVD"
     gene            819..2293
                     /locus_tag="RJE42_002338"
                     /pseudo
     CDS             819..2293
                     /locus_tag="RJE42_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2519..3619
                     /locus_tag="RJE42_002339"
     CDS             2519..3619
                     /locus_tag="RJE42_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877733.1"
                     /translation="METRDKLMSLTQSDKTQQWLMDKSSNQDDIQQLQQQFSQQLDQQ
                     YNALLADEKAKLDQYVEVHQGLESLKEEIESEPITLNIDKLPDIKATMLERAKNDEHS
                     DKIEKLFDRLEQALNGTNRLYTQLSLIGTRTHRITTKNFNLQGLPKVVQHTILPSKFK
                     KVYTVDFKSFEPSVAAYMTQDSKLIDLLNQKDGLYDALLSELGLSDELRVFVKRAFIG
                     SFLFGGNFKNPKFKLNQYVSEVQWLDAVSQFTKVIELKKHVEKSNSMPMPYSIEHDMS
                     AFQGSSIMAIYVQTVASYIFKHILLKVYKAQCDQKTFKIIVPIHDAIMIECEDEEIAQ
                     NVGQLMKDTANQLFNGEFAHVTVEEIGGVDHE"
     gene            3612..3983
                     /locus_tag="RJE42_002340"
     CDS             3612..3983
                     /locus_tag="RJE42_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877734.1"
                     /translation="MNNDRGQSLHIPSSTPIKENNIYVATLHSVIQTDFSGEIKHQFT
                     YEIEVNNQIVYANRNIPTKPSANQLSIHDWLKRHSNYSASHENYEPYIDQKHLILLGQ
                     YNGNYYVQDVASLDAFGGVLS"
     gene            3980..5623
                     /locus_tag="RJE42_002341"
     CDS             3980..5623
                     /locus_tag="RJE42_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV5877735.1"
                     /translation="MNHILQMLSKLLSVAKEAIDRQGLIAILTIPVNNNDEIEETAQG
                     ETVYNELIDQLRLNIPKDTDYQPNIYSYFGIKKNPNDTVLMEMMIKVFHIKRFNSELF
                     IFKVNGWQKINGDELQGLISKMIQVLLVDYKPSLSTLKNVVDGLQKSTDVEELVENER
                     YIGCGENMFDLNTFQVVKNSIDIFPKTRLNLSLSTNDVITDKIPPYFKQYMLQLANYD
                     DDLQYFLFQHTAVLLTADTKYRRGLILYGGAKNGKSVYIELVKSFFYSKDIVSKPLNE
                     LEGRFDKESLIDKSLMASHEIGQSRIQEKIVNDFKKLLSVESMHVDRKGKTQVEVILD
                     LKLIFSTNAILNFPPEHAKALERRINIIPCEYYVEKADTSLIDKLQSEKKEIFLYLMY
                     VYQQIVKADIEYLENSRVTEITHDWLNFGYEFVSSRSVSNANQKACINLLRKLIEIKS
                     GSRIKVSELNKVINEEIKVSSQVIKQLIQANFDTQTKLYNGYDYCIDLGWKEANKKEI
                     HDISEKDNIISLDKNENITDDEALDEENLDFDWEDFDDE"
BASE COUNT         2273 a          740 c          992 g         1679 t
ORIGIN      
        1 caagatgcgc agaaaaaagt attgtacgaa gatatattta tagaagaaaa taaagataac
       61 ttgaaattta atagaattaa cgaaatttgt agcaatgatt tgaatgtttt aggatatata
      121 atcagttatg gattaaccga atctgaagca ttttctgcag aaaatgttct tataaacttt
      181 cttaacctaa caaataaaac tacattaaca aatatgatta acggtcatgg atctaaggca
      241 tacttagttg aagatttaga aaatgaattt ggctacgatt caattaatct tgaaaacata
      301 aatacgaatg aattaatttt agcagtgaaa atcagagatg catttctatt agataaggat
      361 gaaagtaaag agtatcctat taatgaaagt aaacgtgata gaaataacct taaatcgcgt
      421 acattaggta gttggataat aggaaaagat aaaatacata aaataaaata tattattggt
      481 atcaatacag gtgctaataa tgcagttgtc tcagcttatg aagtatcatt tgaacaagcg
      541 gagagtatcg aaacaaataa tggtagaatg agatatgcgt ttattgggct ttcagaaaga
      601 gatgctactc taaaaaaatt gaatttatac aaaaaagcac taccagattt aagatttggt
      661 agtggtagcg ctacagcata tataaataat gggacaatga aagttgatta aatgaataaa
      721 aacatccaat tccttaaatt agcctttgaa aattttatac tcgtatatat aagtgaaaaa
      781 cttaaaagaa ttagatgtta attaaaggag agaatgctat gccatcagat gatattgtaa
      841 aaaaactata ctcaaaagag gatattcgat tagaattagg acttactccg cataagttta
      901 ataaaaaaat ggaaactatt gctaagcttt ttaaaattga tatgaaaatt tttcacaatt
      961 acaaaggcca agataaaaat aatcagtata catttaatgg tgtcgcaaaa gaattaatca
     1021 aagtgttgct aaaaagtgtt gattattacc cagtggatat caattccaaa aaatttaaac
     1081 aaaatggaaa atctaaaaaa gaaatgattg aaaatataga taactctagt tacatgaaat
     1141 atatttatca gttaatgaaa tctatcaatg aaattcaata caaaaggtta attgctgata
     1201 tacatatgaa agatgtgtac cagaatacaa aagcatggtt aaataatggt gaatcaatta
     1261 ataaaaaaga acaagagcta taccaatata tgacaatttt accattacat aaaagagttg
     1321 aactgcaaaa tgaggtatta aaatctatag acgaaacaat ttttcaattt ctcgcaaaag
     1381 aacatagaaa taatcaaatt gaagaaaata atgaattaga agcatataca aaagcaataa
     1441 aagaaggtag aaatcctaaa aatgattatg aattaaatca tctcctatat aaaaaaatca
     1501 attaccctta gatagtctaa tagaagatat atgggactat gaggaaacag agtatactga
     1561 gcttgattgg ttaattgcag atatgctaaa acgttcacaa agagctgata gtaattttat
     1621 cgaaagttta gagaagaaaa acaaattgag aaaaaatatt cataaagatt ccattaaaag
     1681 tatatctaag ttgattgatc atgaattagt taaaaataat agaaagtggg acgtagcaga
     1741 attttataaa aatcaacaat tttggaataa tagtacatta agacgtagct ctcaaaattt
     1801 tggaatttta tattacaaaa agaataggtt acatacaatg aatcaaagta atattattaa
     1861 gaaaataaaa attatcgaag ctaatataga gagtgcaaat aaacagccga gctatttaga
     1921 tttcttttta caagctaagt ttgatttaga gaggttagaa agtgaattaa tgaaatatag
     1981 cattaatccg aaatatttta ataagataaa tagtgattac gtaagatatg ctattgaaga
     2041 tgtacataat gcaattatta aaattgacag atatataagt aacgataaag tgaagttaaa
     2101 ctattcaaat gtccaaatgg tagtgagaaa cgaaataaca gaacaaggaa gctactttgt
     2161 aacccaggca ttgaatacac tgtcaaaggt agaagagtct ggctacagct ttgataattt
     2221 catggtagga cctttcgtag aagactttaa aaaagcaatt aaaaatcgta gatttggttc
     2281 taaaaaagat taagattgtt ttttagagtt aaacataaga ataaatttaa ataaaaaaat
     2341 aaacccaaca aaccttgtta tatcaatgtt tgttgggttt atttttttgg tgatttttta
     2401 ataactcaat tacatcttag gataatatcg ctaaattata cattgtcgag gccaagacaa
     2461 ataaaaaatt aaagcaaaag ttagcgcgca actttctgct ataaaggagc aaatttatat
     2521 ggagacaaga gacaaactga tgtcattgac tcaaagtgac aaaacacagc aatggctaat
     2581 ggacaagtca tctaaccaag atgacattca acaattgcag caacaattca gtcagcagct
     2641 tgatcaacaa tataatgcac ttttagctga tgaaaaagct aagttagacc aatacgtgga
     2701 agtacatcaa ggattggaat cattaaagga agagattgaa tcagaaccta ttacgcttaa
     2761 tatcgataaa ttacccgata tcaaagcaac aatgcttgaa agagccaaga atgatgaaca
     2821 ttctgataaa atcgaaaagc tatttgatag gttagaacag gcattaaatg gtacgaatcg
     2881 attatatacg caattatcgt tgattggtac acgaacacat cgaattacaa ctaaaaattt
     2941 taatcttcaa ggcttaccta aagtagtcca acatacgatt ttaccttcaa aatttaagaa
     3001 ggtgtataca gtcgatttta aatcgtttga accatcagtt gcagcgtaca tgactcaaga
     3061 ttcaaaactg attgacttgt taaatcagaa agacggactg tatgacgcat tgctaagtga
     3121 attaggctta tcagatgagc tacgtgtatt tgttaaacgt gcatttattg gttcgtttct
     3181 atttggaggt aacttcaaaa atcctaaatt caagctgaat caatatgtaa gtgaagtaca
     3241 atggttggat gcggtcagcc aatttacaaa agtcattgaa cttaagaagc acgttgaaaa
     3301 gagtaattcc atgcctatgc cttatagtat tgagcatgat atgagcgcat ttcaaggtag
     3361 cagtattatg gcaatctacg tacaaactgt agcgagttat attttcaagc acattctgct
     3421 aaaagtgtac aaagcacagt gcgatcaaaa aacgttcaag attatagtac ctatacacga
     3481 tgcgattatg attgaatgtg aagatgaaga aattgcacaa aatgtgggtc agttaatgaa
     3541 agatacggct aaccagttgt tcaatggtga atttgcacat gtgacagtgg aagaaatagg
     3601 aggcgtagac catgaataat gatagaggac aaagcctaca catcccaagt agtacaccaa
     3661 tcaaagaaaa taatatatat gtagctacgt tacattctgt gatccaaaca gatttctcag
     3721 gtgaaataaa gcaccaattc acgtatgaaa ttgaagtgaa caatcagatt gtatatgcga
     3781 atcgtaatat tccaacaaaa ccgagcgcta atcagttgtc aattcatgat tggctgaaac
     3841 gtcatagcaa ctatagcgca agtcatgaaa actatgagcc ttatattgat cagaaacatt
     3901 taattctatt aggtcaatat aacggtaact attatgtaca agatgtagca tcgttagatg
     3961 cgtttggagg cgtattatca tgaatcatat attacaaatg ttatctaagc tattaagtgt
     4021 ggccaaggag gcaatcgacc gtcaaggtct gattgctatc ctaactattc ctgttaataa
     4081 taacgatgaa atagaagaaa cggctcaagg tgaaaccgtg tataacgaac ttatcgatca
     4141 gttacgactt aatatcccaa aagatacgga ttatcaacct aacatctata gttattttgg
     4201 tattaagaag aatcctaatg acaccgtact catggaaatg atgataaagg tttttcatat
     4261 caaacgcttt aattcagaac tgtttatttt caaagttaac gggtggcaaa agataaatgg
     4321 agatgaatta caagggttga tatctaaaat gatacaagta ttgcttgtag attataagcc
     4381 ttcactaagc actctaaaaa atgtcgtaga tggattgcaa aaatcaacag atgtagaaga
     4441 acttgttgag aatgagcgct atattggttg tggtgaaaat atgttcgatc ttaatacgtt
     4501 tcaagtcgtt aaaaattcaa tcgatatctt tccaaaaaca cgattgaatt tatcattaag
     4561 tacaaatgat gtaattactg ataagatacc gccttatttt aagcaatata tgttacaact
     4621 tgcgaattat gacgatgatt tacaatactt tcttttccaa catacagcag tattacttac
     4681 agctgatact aaataccgta ggggtctcat attatatggt ggagctaaga atggtaaatc
     4741 tgtatatatt gaactagtta aatcattttt ctatagtaaa gatattgtgt ctaagccact
     4801 taatgagctt gaaggtcgtt ttgacaaaga aagtttaatt gacaaaagtc taatggcaag
     4861 tcatgaaatt gggcaatcta ggattcaaga aaagatcgta aatgacttca aaaagttatt
     4921 atctgtagaa tcaatgcatg ttgatcgtaa aggaaaaact caagtggaag tcattttgga
     4981 tttgaaactt atttttagta caaatgcgat acttaatttt cctcctgaac atgcgaaagc
     5041 tttggagcgt cgaattaata ttattccatg tgagtattat gttgaaaaag cggacacttc
     5101 attaattgat aagctccaga gtgagaagaa agaaatcttt ctttacttga tgtatgtgta
     5161 tcaacagatt gtaaaagcag atatcgagta tcttgaaaat agccgtgtca ctgaaattac
     5221 tcacgattgg ttaaattttg gatatgaatt tgtttctagc aggtccgtaa gtaatgcaaa
     5281 tcagaaagca tgtattaatt tactcagaaa acttatagaa atcaaatcag gatcacgaat
     5341 caaagtatcc gagttaaata aagttattaa tgaagaaata aaggtaagtt ctcaggttat
     5401 taaacagtta attcaagcaa actttgatac tcaaaccaaa ctatacaatg gctacgatta
     5461 ttgtattgat ttaggttgga aagaagccaa taaaaaagag attcatgata tttcggaaaa
     5521 agataatatt atttcattag ataaaaatga aaatataaca gacgatgagg cattagatga
     5581 agagaatttg gactttgatt gggaggactt tgacgatgaa taatgaacaa attgaagcat
     5641 ttgtagaagt gcttgtacct atcatagaag aacgtatcaa taaa
//
LOCUS       DAPIDM010000150         5638 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000150 DAPIDM010000000
VERSION     DAPIDM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.150"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            10..1974
                     /locus_tag="RJE42_002342"
     CDS             10..1974
                     /locus_tag="RJE42_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDV5877736.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKAKATSITPFNAKY
                     NIHYLSDDELLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESFKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IISQILLTTSVLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDYRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILVIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYRYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            1977..2297
                     /locus_tag="RJE42_002343"
     CDS             1977..2297
                     /locus_tag="RJE42_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDV5877737.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            2294..2935
                     /locus_tag="RJE42_002344"
     CDS             2294..2935
                     /locus_tag="RJE42_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV5877738.1"
                     /translation="MIELKDLTIQKGNTHILNKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVYENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPETGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASL"
     gene            complement(3023..3313)
                     /locus_tag="RJE42_002345"
     CDS             complement(3023..3313)
                     /locus_tag="RJE42_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877739.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYAA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILLFFSLPLLLS"
     gene            3403..3591
                     /locus_tag="RJE42_002346"
     CDS             3403..3591
                     /locus_tag="RJE42_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HDV5877740.1"
                     /translation="MEDNKNGCLIDNHDINDMADKILKLVNNDVLAEEFGSKARENII
                     EKYSTESILEKWLNLFNS"
     gene            complement(3680..4036)
                     /locus_tag="RJE42_002347"
     CDS             complement(3680..4036)
                     /locus_tag="RJE42_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV5877741.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            4527..5486
                     /locus_tag="RJE42_002348"
     CDS             4527..5486
                     /locus_tag="RJE42_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV5877742.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTDMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGTYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTAHVGERKGGPYIYLNNEELANINPKVMILATNGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLQSRGIIASESMAEDLEKIAEKAK"
     gene            complement(5535..>5638)
                     /locus_tag="RJE42_002349"
     CDS             complement(5535..>5638)
                     /locus_tag="RJE42_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877743.1"
                     /translation="KRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHK"
BASE COUNT         2222 a          684 c          923 g         1809 t
ORIGIN      
        1 aggtacaaca tgaaatggtt caaattaata cttgatgtta caatcttcat tctaattgct
       61 atattgttat ttgtttatac atataaagaa aacgaagaaa tattgcctga tactaaatac
      121 cctatagcgg taactgactg gaataaaaaa tatagtaaaa atgagattta taaacgtata
      181 aatcaattcg caaaaaatga gaacgtagca atctataaat caacttcaaa ttatacaaac
      241 aaaaacgtag ataaagatat atatgtattt aataaagcaa aagcaacatc tatcactcct
      301 tttaatgcta aatataacat tcattattta agtgacgacg aattactaaa aaaggatatc
      361 aaagggagtt attttgtaaa agacaaaaat tttgacgtgt ctaaattcat aaatttttta
      421 aaagaatatg gtgttactgc tgaatcattc aaaatagatc atatgatgat tgccgttggt
      481 gtcattaaac aaatgaatat agtagttccg ttatcctcac ttttaatcgt ttattttatt
      541 tattatattt tcgaaaaaaa cattaatttc aaagcgtatg cgattaagta tttaaatggt
      601 tttacattaa gaaaaataat ttttgaaaat ttttcgaaaa aatgcactta ttgggtaacg
      661 ttaatcataa gtcaaattct tttaactaca agtgtacttt ggatattaaa ttacacgggt
      721 aatttagatt tattcatatt aagattagtt ctgctttcat gtcttttcat tttaacgatt
      781 agtgttatta atttatggac tttcttaatg ttactaaatc taaatattgc caatatgatt
      841 aaaggtaagc agcactttaa aacaattcgt tttattaata cagtttgtaa aagtattctc
      901 ttagtactaa tagctagtgt aatgatagaa aatactagtg ttattaaaga tttgaataaa
      961 ataaaggaaa ctgagaaata ttggaatgta ttagatgatt attacacgat tgaatttgca
     1021 ccttatcacg aaacgaaaca aagtttgatt gataatatgc tgcgatcaga acaattagta
     1081 aaggctagtg aagcagaaaa taatgcgatt ttattcaaac caaagggtga ctccgttgac
     1141 aatgacaact tttcgcctga tgaggggaat gtaatattag taaataatca gttctggtcg
     1201 atttatcata agcagtttca acctgatatt ccgataaaaa atcaaaaaaa taatgtcgaa
     1261 gtaattattc cacaaaagtt tcatgcaatg cgtaatgaaa tcaatcaagc atatcattca
     1321 tggtttgaat ttgtacaaaa taaaaataat aaagagaata agttatctat acagtttatc
     1381 aacaaaaatg attatcgaat tttttcattt gatgcacgag atagtcgcca tttgtcattt
     1441 atagaggcgc caatcattgt gaatgttcag gcatcagatt tatcgaatga tttttattat
     1501 gccatgatca gtcaaggcgg gtatttattc aaaaattatg acgcgctagt aaaaaatatt
     1561 gaaaagtatc atcttgatgg ggaaatcagt ggaataacca attataaaga tagcgtaatg
     1621 gaaatgtatc atgaaaacaa tttgaaatta actgtactca acttttcaca aatcattatc
     1681 gcaatcattt tagtaattat tattttattt gatgtgaaat attattttga acagcatcga
     1741 aaattactcg taatcaaaaa gctatatggt tattcaacat taagagccaa ttaccgatac
     1801 ttattaataa ataatatagt tgttattttt attggaatat tgacgaatgt aattttacat
     1861 tctcactata taatgatgtt atttgcaacg attcttgtcg ttcaaatatt attgcagatt
     1921 tgcagcctat actatcatgg ccgacgtttt aatgaagtta tcaaggagtt ttaactatga
     1981 aatttatcat tgcaatatta ttaggattaa tattatccat tactattgct tttacaatca
     2041 tacatcatcc tatacttgat cgttttaatc ctttcttaaa aacggagtac agttatgcca
     2101 aagtgccaaa aggtacgcaa caatatgtga atattacggc ttatagtgaa agaggggaaa
     2161 agcttgatta taaattaaca tttaatggat tttcacctag tagaacgtat gtcgaaataa
     2221 agcataaagg gcaatatgtc atatcgatca catatgttga aaaagaggat acaccaaaag
     2281 aggtaagaca agaatgatag aattaaaaga tttaaccata caaaaaggta atacacatat
     2341 tttgaataaa cttaatttga aatttcaatg tggaaaatca tatgcactta ttggtaaaag
     2401 tggatgcggt aaatctacat tattaaatac tattgctgga cttgaaaaaa cagggaaaca
     2461 atatgtctat tttaatggtc aattagaaca atttaaatct aatttttaca gagataaatt
     2521 agggtattta tttcaaaatt atggattaat cgataatctg acagtatatg aaaatttaga
     2581 tattggatta gcatataaaa aaataagtaa gaaagaaaaa gaacaattaa agatacgtta
     2641 tatagaacag tttggtctgt caaacagttt aaaaagaaaa gttcatacgc taagtggagg
     2701 tgaacaacaa cgtgtcgctt taattagaat gatgttaaaa gatccgattg ttatgttagc
     2761 tgatgaacca actggtgcgt tagatcctga aacaggacag atgattattc aatcattatt
     2821 tgatttggtc gatgaaaata aagtgctgat tttagcaaca catgatatgg ctattgcaaa
     2881 tcaatgtgat gaaataatag atttagaaca gtatagaaaa gtagcatctt tgtgatatcc
     2941 aaaagataaa attaaaagcc aatcaatggg aaatgttgac ttcaacgctc cgtttgattg
     3001 gcttttataa tgtgttacta agttatgaaa gtaacaacgg caaactgaaa aaaagcaata
     3061 taataatatt taatacgagt agtaataaat gtaattgatt ttttgtttta atgtagcaaa
     3121 aaacaatgac taaagatagc agcaatatca tgattgcacc tgggaagaac cataatgctg
     3181 aagctgcata ccaaatcatt ggtaacgcca taataataaa agatataaga caagaaagga
     3241 taccaataat attataagtt ctagtcaaaa ttacacctcc aattttcatt ttaaaatgac
     3301 atgcataaaa caatcatttt aaatacatca tacaataaaa ataaattgta tgatagatga
     3361 atttgaaatt aaattgaatt ggcttgatta aaaagtgatt ttatagaaga taataaaaat
     3421 ggttgtttaa tagataacca tgatattaac gacatggctg ataaaatact gaagcttgtt
     3481 aataatgatg tattagcaga ggagtttggt tcgaaagcga gagaaaacat tatagaaaaa
     3541 tattcaacgg aatcaatatt agaaaaatgg ttaaatcttt tcaatagcta aatgaaaaaa
     3601 tcctaggtat tcatgagtac ctaggatttt aatacatgat atttacaaaa aaagagagga
     3661 tagtcctctc aaataatagt taataaaatt gtaaccattt cttccagctg taactactaa
     3721 tacaagctaa gttcattacg atgtgtatga tttcaaattc ataaccactt acatatcctc
     3781 ttgtaagttt aagtgtgaag attgagccca acataacaca aatataaaat acattaacat
     3841 atggcgttaa aaagccgata ataagtgcta aacctccaac aacttcgcca attgacatca
     3901 atactgcgat cgtaggtggt aaacctaaag aacctaagaa atgaattgtt ccatctaaat
     3961 ctacaaattt caaagtaccg tgtactaaga atgatgttgc tagcatccat ctaattaaaa
     4021 gcatacctaa tttcatcatg tcacctccaa catacatttt tttcttaaaa catagtccaa
     4081 attatatttg taaagatgtg gaaagtaaag attatttata aaaataatta ttaaatattt
     4141 attaaatgtt ctattgtatt gaacaattgt tgtagaaaga tgtttaagtc atcagttact
     4201 ttttgtattt ataaataaag attagtacag catagtgcaa agtttatagg taaatgaatt
     4261 aattatggca tcaaaatata cgtatcttga acactgttgt ggaggcttag tagaagtgag
     4321 aacaaacaga atgctacttt aagcgatatg gttaatatta attagtgcgt cattttcaaa
     4381 attagaggtc atttgctgta taggattaga aattttgata atcaactatg ataagaaaaa
     4441 ataatgacca tttaaatata ttataagttt attaaatgat aaaataataa tatagttctc
     4501 tataaaaaat gtttggaggt aaatgtgtga ataggaatat cgttaaacta gttgtgttta
     4561 tgctaatctt agttgtagca gtagcgggtt gtggtcaaaa agatactgaa gagaaaactg
     4621 atatgacgac aataaaagat gaattaggaa ctgaaaaaat taagaaaaat ccgaaacgtg
     4681 ttgttgtatt agaatatagt tttgctgatt atttagcagc attagatatg aaacctgttg
     4741 gtattgcaga tgatggcagt actaaaaata taacaaagtc agtaagggat aaaattggaa
     4801 catatgaatc ggttgggtct agaccacaac cgaatatgga agtgataagt aaattaaaac
     4861 cggatttgat tattgcagat gtcagcagac ataagaaaat caaatcagaa ttgagcaaaa
     4921 ttgcgccgac aattatgtta gtcagtggta cgggagatta caatgcaaat attgaagcat
     4981 ttaaaacagt cgctaaagca gttggcaaag agaaagaagg cgaaaagcgt ctggaaaagc
     5041 atgataaaat attagcggag attagaaaga aaattgaaca gagtacgtta aaatctgcat
     5101 ttgcatttgg tatctcaaga gcaggtatgt ttattaataa tgaagataca tttatgggac
     5161 aattcttaat taaaatgggt attcaacctg aagtcacaaa agacaaaact gcgcatgttg
     5221 gtgaacgcaa gggtggccct tatatttatt taaataatga agagcttgcc aatatcaatc
     5281 caaaagttat gattttagct acgaatggaa aaacagataa aaatagaacg aaattcattg
     5341 atcctgcagt ttggaaatca ttaaaagctg tgaaagataa taaagtttat gacgttgacc
     5401 gaaataagtg gttgcaatca agaggtatta tcgcaagtga aagtatggca gaagatttag
     5461 agaaaattgc tgaaaaagca aaataataaa atatagcgtc gctcgtttat caaatgtgag
     5521 agacgctata ttttttattt atgtttatta tctgtcggat gtgatgattt acctgaaagt
     5581 ttatttcgaa taaatttaat tacataaccg acaaggattg ttttaacaat tcttttta
//
LOCUS       DAPIDM010000151         5596 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000151 DAPIDM010000000
VERSION     DAPIDM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.151"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            251..649
                     /gene="nrdI"
                     /locus_tag="RJE42_002350"
     CDS             251..649
                     /gene="nrdI"
                     /locus_tag="RJE42_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV5877744.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            612..2717
                     /gene="nrdE"
                     /locus_tag="RJE42_002351"
     CDS             612..2717
                     /gene="nrdE"
                     /locus_tag="RJE42_002351"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV5877745.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2837..3808
                     /gene="nrdF"
                     /locus_tag="RJE42_002352"
     CDS             2837..3808
                     /gene="nrdF"
                     /locus_tag="RJE42_002352"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV5877746.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4178..5149
                     /locus_tag="RJE42_002353"
     CDS             4178..5149
                     /locus_tag="RJE42_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877747.1"
                     /translation="MKYLLKGKILLLLLILLTIISLFIGVSELSIKDIFHLSDSQQNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSISGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5136..>5596
                     /locus_tag="RJE42_002354"
     CDS             5136..>5596
                     /locus_tag="RJE42_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDV5877748.1"
                     /translation="MRNNNKKIMLLIAVTFLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQ"
BASE COUNT         1996 a          785 c          963 g         1852 t
ORIGIN      
        1 aaaatgtact gagcaaatgg aagatatcta atgatctaaa cactacatat agtgattttt
       61 atacattcaa cccatataag ctactatttt ctcaaatata aatctatgca attggtttac
      121 atttgagaaa ataagtagct tcattatagt taatacaatg ctgagataac catagtagcg
      181 acgttgttaa agcatttttt aattgtaatg actacttaat ttaaaagggt tgaagaaaga
      241 aggtgatcca atgaaaataa tatatttttc atttactgga aatgtccgtc gtttcattaa
      301 gagaacagaa cttgaaaata cgcttgagat tacagcagaa aattgtatgg aaccagttca
      361 tgaaccgttt attatcgtta ctggcactat tggatttgga gaagtaccag aacccgttca
      421 atctttttta gaagttaatc atcaatacat cagaggtgtg gcagctagcg gtaatcgaaa
      481 ttggggacta aatttcgcaa aagcgggtcg cacgatatca gaagagtata atgtcccttt
      541 attaatgaag tttgagttac atggaaaaaa caaagacgtt attgaattta agaacaaggt
      601 gggtaatttt aatgaaaacc atggaagaga aaaagtacaa tcatattgaa ttaaataatg
      661 aggtcactaa acgaagagaa gatggattct ttagtttaga aaaagaccaa gaagctttag
      721 tagcttattt agaagaagta aaagataaaa caatcttctt cgacactgaa atcgagcgtt
      781 tacgttattt agtagacaac gatttttatt tcaatgtgtt tgatatttat agtgaagcgg
      841 atctaattga aatcactgat tatgcaaaat caatcccgtt taattttgca agttatatgt
      901 cagctagtaa gtttttcaaa gattacgctt tgaaaacaaa tgataaaagt caatatttag
      961 aagactataa tcaacacgtt gcaattgttg ctttatacct agcaaatggt aataaagcac
     1021 aagctaaaca atttatttca gctatggttg aacaaagata tcaaccagcg acaccaacat
     1081 ttttaaacgc aggtcgtgcg cgtcgtggtg agctagtgtc atgtttctta ttagaagtgg
     1141 atgacagctt aaattcaatt aactttattg attcaactgc aaaacaatta agtaaaattg
     1201 gtggcggcgt agcaattaac ttatcaaaat tgcgtgcacg cggtgaagcg attaaaggaa
     1261 ttaaaggcgt agcgaaaggt gttttaccta ttgctaagtc acttgaaggt ggctttagct
     1321 atgcagatca actaggtcaa cgccctggtg ctggtgctgt gtacttaaat atcttccatt
     1381 atgatgtaga agaattttta gatactaaaa aagtaaatgc ggatgaagat ttacgtttat
     1441 ctacaatatc aactggttta attgttccat ctaaattctt cgatttagct aaagaaggta
     1501 aagactttta tatgtttgca cctcatacag ttaaagaaga atacggtgtt acattagacg
     1561 atattgattt agaaaaatat tatgatgata tggttgcaaa tccaaatgtt gagaaaaaga
     1621 aaaagaatgc acgtgaaatg ttgaatttaa ttgcgcaaac acaattacaa tcaggctatc
     1681 catatttaat gtttaaagat aatgctaata gagtgcatcc gaattcaaat attggacaaa
     1741 ttaagatgag taacttatgt actgaaattt tccaattaca agaaacttca attattaacg
     1801 actatggtat tgaagacgaa attaaacgtg atatttcttg taacttaggt tcattaaaca
     1861 ttgtaaatgt aatggaaagc ggaaaattca gagattcagt tcactctggt atggacgcat
     1921 taactgttgt aagtgatgta gcaaacattc aaaatgcacc aggagttaga aaagctaaca
     1981 gtgaattaca ttcagttggt cttggtgtga tgaatttaca tggttactta gcaaaaaata
     2041 aaattggtta tgagtcagaa gaagcaaaag attttgcaaa tatcttcttt atgatgatga
     2101 atttctactc aatcgaacgt tcaatggaaa tcgctaaaga acgtggtatc aaatatcaag
     2161 actttgaaaa gtctgattat gctaatggca aatatttcga gttctataca actcaagaat
     2221 ttgaacctca attcgaaaaa gtacgtgaat tatttgacgg tatggctatt cctacttctg
     2281 aggattggaa gaaactacaa caagatgttg aacaatatgg tttatatcat gcatatagat
     2341 tagctattgc tccaacacaa agtatttctt atgttcaaaa tgctacaagt tctgtaatgc
     2401 caatcgttga ccaaattgaa cgtcgtactt atggtaatgc ggaaacattt taccctatgc
     2461 cattcttatc acctcaaaca atgtggtact acaaatctgc attcaatact gatcagatga
     2521 aattaatcga tttaattgcg acaattcaaa cgcatattga ccaaggtatc tcaacgatac
     2581 tttatgttaa ttctgaaatt tctacacgtg agttagcaag attatatgta tatgcgcact
     2641 ataaaggatt aaaatcactt tactatacta gaaataaatt attaagtgta gaagaatgta
     2701 caagttgttc tatctaataa gtaaacgtaa aaaatgacaa acaactaatc atctcgttta
     2761 aaataacaga tgattagtct gccatgtcta tatttgtcaa ttattgagag taacattaca
     2821 ggaggaaatt atattcatga tagctgttaa ttggaacaca caagaagata tgacgaatat
     2881 gttttggaga caaaatatat ctcaaatgtg ggttgaaaca gaatttaaag tatcaaaaga
     2941 cattgcaagt tggaagactt tgtcagaagc tgaacaagac acatttaaaa aagcattagc
     3001 tggtttaaca ggcttagata cacatcaagc agatgatggt atgcctttag tcatgctaca
     3061 tacgactgac ttaagaaaaa aagcagttta ttcatttatg gcgatgatgg agcaaataca
     3121 cgcgaaaagt tattcgcata ttttcacaac gctattacca tctagtgaaa caaactattt
     3181 attagatgaa tgggttttag aagagccaca tttaaaatat aaatcagata aaattgttgc
     3241 taattaccac aaactttggg gtaaagaagc ttccatatat gaccaatata tggccagagt
     3301 tacgagtgta tttttagaaa cattcttatt cttctcaggt ttctattatc cgctatatct
     3361 agctggtcaa ggaaaaatga cgacatcagg tgaaatcatt cgtaaaattc ttttagatga
     3421 atctattcat ggcgtattta ccggtttaga tgcacagcat ttacgaaatg aactttctga
     3481 aagtgagaag caaaaagcag accaagaaat gtataaattg ctaaatgact tgtatttaaa
     3541 tgaagagtca tacacaaaaa tgttatacga tgatcttgga atcactgaag atgtgttaaa
     3601 ctatgttaaa tataatggaa ataaagcact ttcaaactta ggctttgaac cttattttga
     3661 ggaacgtgaa tttaacccaa tcattgagaa tgccttagat acaacaacta aaaaccatga
     3721 cttcttctca gtaaaaggtg atggttatgt attagcatta aacgtagaag cattacaaga
     3781 tgatgacttt gtatttgaca acaaataata attaaaatta aaaagacctt cacatgaaaa
     3841 ggcaaagcga tgacattcgc ttccgacatg ttgaaggtct tttttctatg cttaaaaacg
     3901 tatacatatt ttgaaaaagt aaaaatgaca tttaatgatg tcaatgagtc tagaaagtat
     3961 acaagatatt gcattacaaa agggtgtgct attttactat atacatctaa attacacagt
     4021 tacaaaaatt gatttctatt ttaattgcaa atatcaataa aattgacaat aaattatttg
     4081 aaaggctatt gaaatgagtg acaaaaaacg ataccattaa tgagaaatgt tatcaatgat
     4141 aataattatc attaaattaa agggagaaaa ttttgtaatg aagtatttat taaagggaaa
     4201 aattttgctt ctattactta tattgctaac aatcatttcg ttgtttatag gtgtcagtga
     4261 actgtctatt aaagatatct ttcatttatc agactcacag caaaatatat tgttctcaag
     4321 ccgaataccg agaacgatga gtattttaat tgctggaagt tcattagctt tagctggcct
     4381 aatcatgcaa caaatgatgc aaaataagtt tgttagtcca actacagctg ggacgatgga
     4441 gtgggcaaaa ttaggtatct taattgcctt attattcttt ccaactggtc atattttatt
     4501 aaaactagta tttgctgtta tttgcagcat cagcggaaca tttttatttg ttaaaattat
     4561 tgattttatc aaagtgaaag atgtcatctt tgtaccacta ttagggatta tgatgggtgg
     4621 cattgtcgct agttttacaa cgtttatctc attacgtaca aacgcagttc aaagcattgg
     4681 taactggctt aacgggaact ttgccattat cacaagtgga cgttatgaaa tcttgtattt
     4741 aagtattcct cttttagcat tgacatatct ttttgcaaat catttcacaa ttgtaggaat
     4801 gggtaaagac tttaccaata atttaggttt gagttatgaa aaattaatca atattgctct
     4861 atttattact gccacaatta cggcattagt cgtcgtgaca gttggaactt tacccttctt
     4921 aggtttagtt attccaaata ttatttcgat ttatagaggc gatcatttaa aaaatgcaat
     4981 tcctcatacg atgatgttag gtgccatctt tgtattgttt tctgatatag ttggcagaat
     5041 tgtagtttat ccatatgaaa ttaatattgg tttaacaata ggtgtatttg gaacaatcat
     5101 tttccttatc ttacttatga aaggtaggaa aaattatgcg caacaataat aaaaaaataa
     5161 tgcttttaat tgcagtcacg ttcttaatta gtatgctgta cttatttgtt ggtattgatt
     5221 ttgaaatatt tgaatatcaa ttttcaagtc gtttaagaaa gttcatatta attattttag
     5281 taggtgctgc cattgcaaca tccgttgtta ttttccaagc gattacaaat aatcgtttat
     5341 tgacaccatc aataatggga ttagatgcag tttatttatt tatcaaagta ttgcccgtct
     5401 ttttatttgg aattcaatcg gtatgggtta ctaatgtata tttgaacttt atattaacac
     5461 ttataacgat ggtattattc gcactaatcc tattccaagg tatctttaaa attggacatt
     5521 tttcaattta ctttatctta cttataggtg tccttttagg aacatttttt agaagtatta
     5581 caggttttat tcaatt
//
LOCUS       DAPIDM010000152         5595 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000152 DAPIDM010000000
VERSION     DAPIDM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.152"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            252..1292
                     /locus_tag="RJE42_002355"
     CDS             252..1292
                     /locus_tag="RJE42_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HDV5877749.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRCLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1306..2373
                     /locus_tag="RJE42_002356"
     CDS             1306..2373
                     /locus_tag="RJE42_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDV5877750.1"
                     /translation="MWNKNRLTQMLSIEFPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSNQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2716..3564
                     /locus_tag="RJE42_002357"
     CDS             2716..3564
                     /locus_tag="RJE42_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV5877751.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQSFPMCVEFRHQSWFSDAFKEQTLSFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3577..4404
                     /locus_tag="RJE42_002358"
     CDS             3577..4404
                     /locus_tag="RJE42_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV5877752.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4431..>5595
                     /locus_tag="RJE42_002359"
     CDS             4431..>5595
                     /locus_tag="RJE42_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV5877753.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLDSIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVKTSFEEEGKTLMSNPVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLN"
BASE COUNT         1996 a          834 c         1048 g         1717 t
ORIGIN      
        1 tcattataac tctgtcataa actgaatttg ttgaaatttt tcattatgta aatttattcg
       61 taacaatcag gtcgaactat agcatcattt tactaatgaa tgcattaaag taactatgat
      121 taaaaatgca tattaattat cattattaag tctattatat ataatgaatt ttaactggtt
      181 tattaaacga gaacgtcggg aattaggtaa ctacaataaa aataagatat gacaataagg
      241 agactacacg cgtgatcatt gccataatta tattgatatt tatttcgttt ttcttttcag
      301 gaagcgagac ggcattaacg gctgccaata aaacaaaatt taaaactgaa gctgacaaag
      361 gtgataaaaa agcaaaaggc attgtaaagt tacttgaaaa accaagtgag tttattacaa
      421 cgattctaat tgggaataat gtcgcgaata ttttattacc aacacttgtt acaattatgg
      481 ctttacgttg ggggattagc gttgggattg catcagctgt tttaacagtt gttatcattt
      541 tgatttccga agtgattccc aagtctgtcg ctgcaacatt tccagataaa ataacaaggc
      601 ttgtatatcc aattattaat atttgtgtaa ttgtgttccg tcctatcaca ttacttttaa
      661 ataagttgac ggacagtatt aatcgatgtt tatctaaggg ccaacctcaa gaacatcaat
      721 tttcaaaaga agaatttaaa acaatgttag caattgctgg acatgaaggt gctttaaatg
      781 aaattgagac gagtaggttg gaaggtgtca ttaattttga aaatttaaaa gtaaaagatg
      841 ttgatacaac acctagaatt aatgtgacgg catttgcttc aaatgcgaca tacgaagaag
      901 tttatgaaac ggttatgaat aagccataca ctaggtatcc agtgtacgag ggagatattg
      961 ataacattat tggagtgttt cattctaaat atctgttggc ttggagtaat aaaaaagaag
     1021 atcaaattac aaactattca gctaagccat tatttgtgaa tgaacacaat aaagctgaat
     1081 gggtattacg taagatgact atttctagaa aacatttagc aattgtgttg gacgaatttg
     1141 gtggtactga agcgatagtg tcacatgaag acttaatcga agaattatta ggtatggaaa
     1201 ttgaagatga gatggataaa aaggaaaaag aaaaactttc tcaacagcaa attcaatttc
     1261 aacaacggaa aaatcgcaac gtatctatat aaggagcaaa cagctatgtg gaataagaat
     1321 cgacttactc aaatgttaag tattgaattt ccaattatac aagcaggtat ggcaggaagt
     1381 acgacaccga aattagttgc atcagtaagt aacagtggtg gattaggcac aataggcgca
     1441 ggttacttta atacgcagca attagaagac gaaatagatt atgtacgcca attaacgtca
     1501 aattcttttg gtgtaaacgt ctttgtacca agtcaacaat catataccag taatcaaatt
     1561 gaaaatatga atgcatggct aaaaccttat cgacgcgcat tacatttaga agagccggtt
     1621 gtaaaaatta ccgaagaaca acaatttaag tgtcatattg atacgataat taaaaagcaa
     1681 gtgcctgtat gttgttttac ttttggaatt ccaaatgaat ctattataaa aagattgaaa
     1741 gaagcaaaca ttaagctaat aggtacagca acaagtgttg atgaggctat tgcgaatgaa
     1801 aaagcaggta tggatgctat cgttgctcaa ggtagtgaag caggtggaca tcgtggttca
     1861 tttttaaaac ctaaaaatca attacctatg gttggaacaa tatctttagt gccacaaatt
     1921 gtagatgtcg tttcaattcc ggtcattgcc gctggtggaa ttatggacgg tagaggagtt
     1981 ttggcaagta ttgtcttagg tgcagaaggg gtacaaatgg gcaccgcatt tttaacatca
     2041 caagatagta atgcatcaga actactgcga gatgcaatta taaatagtaa agaaacagat
     2101 acagtcatta caaaagcgtt tagtggaaag cttgcacgcg gtatcaacaa taggtttatc
     2161 gaagaaatgt cccaatacga aggcgatatc ccagattatc caatacaaaa tgagctaaca
     2221 agtagcataa gaaaagccgc agcaaacatc ggcgacaaag agttaacaca tatgtggagt
     2281 ggacaaagcc cgcgactagc aacaacgcat cccgccaaca ccatcatgtc caatataatc
     2341 aatcaaatta atcaaatcat gcaatataaa taatcgaccg caatccacaa aagcacaagc
     2401 acccccaaac attattttag tgcttgccat ttttgtggat tgcgtttcta ttttaccaat
     2461 ctaatcaaac gaaattatcg agctgaaggt caccgaaatg attataatca aacgaaatta
     2521 tcgagctaaa gttcactgaa atgtttatga tcaaacgaaa ttatcgagct aaagttcact
     2581 gaaatgttta tgatcaaacg aaattatcga gctaaagttc gctgaaatga ttatgatcaa
     2641 acgaaattat caaactaaag ttcgctgaaa tgattatgat aaaagttata tggtatgatg
     2701 acattggtga tatatatgat aaacatcgga ttaacaggtt ggggtgatca ctattcatta
     2761 tatgaagatt tagaacgcca aaccgataaa cttaaaacat atgctggaca ttttccggtt
     2821 gtcgaattag atgcgacata ctatgcgata caaccggaaa gaaatatatt gaaatggata
     2881 aaagaaacgc ctgatacatt tgaatttgtg gtcaaaattc atcaagcact cacattgcat
     2941 gcagactaca aaacatttgc agatacaagg caagaactat ttgatcaatt taagaatatg
     3001 ttagagccct tacatacaca gaaaaaatta gcaatggtat tggttcaatt tcctccatgg
     3061 tttgactgca atgcacaaaa tatcaaatat attttgtatg taagacagca attacaatca
     3121 tttccaatgt gtgtagaatt taggcatcaa tcatggttta gtgatgcatt taaagaacaa
     3181 acattgtcat ttttaacaga gcatcaaatc attcatgcag tagttgatga accacaagtg
     3241 aaagatggca gtgtaccttt agtcaatcga atcacaaatg aaattgcgtt tgtacgttat
     3301 catggacgta atcattacgg ttggactaag aaagatatgt cagatcaaga atggcgagat
     3361 gtacgctatt tatatgatta taatgagcaa gaattaatag acttggcaca aaaggcacaa
     3421 atattagcac aaaaagctaa gaaagtttac gtcatattta acaataattc tggtggtcat
     3481 gcagcaaata atgccaaaac atatcagcga ttattgaata tagaatatga agggttagca
     3541 ccacaacaat taaaattatt ttaagaggcg acgactatgt tattaacaat tacattatta
     3601 gttttaatcg gaggtttgtc agcgattata gggtcaatcg taggcattgg aggcggtatt
     3661 attatcgttc caacaatggt ttacctcggt gttgaacatg gattactaca taatattaca
     3721 acacaagtag cgatagggac gtcttcagtc attctaattg tgacaggact ttcttcatca
     3781 cttggatatc taaaaacaaa acaagttgat attaaaaatg gctctatctt tttatttgga
     3841 ttattaccag gttcattgct tgggtccttt attagtagat atttaacatt tgagtcattt
     3901 aatttatatt ttggtatctt tttaattttc gtagccattt tattaatggt aagaaataag
     3961 attaaaccgt ttaaaatttt cgataaaccc aagtatgaaa agacttatgt agatgctaaa
     4021 ggtaaaacat atcattatag tgtaccgcct ttattcgctt ttattacaac gtttttaatt
     4081 ggtatattga caggtttatt tggtattgga ggtggcgcac tgatgacgcc actaatgctt
     4141 attgtattta gatttccacc tcatgtagct gttggaacaa gtatgatgat gattttcttt
     4201 tcaagtgtca tgagttctat agggcacatt gctcaaggtc acgtagcttg gggttatgca
     4261 atcattttaa ttatatctag ttattttggt gcgaaaatcg gtgtcaaagt gaatcaatca
     4321 attaagtcag atacggtagt aacattattg agaacagtaa tgttgttaat gggtatatat
     4381 ttaattattc gtgcgttgat ttaatacaac tttaaaagga ggacgtcaat ttgaggctta
     4441 caatttatca tacgaacgat attcatagtc atttacatga atatgaacgc attaaagcat
     4501 atatggcaga acataggcca cgacttaatc atccttcttt atatgttgat ctaggtgatc
     4561 atgtagattt atccgcacct ataacagaag caactttagg taaaaagaat gtagcattac
     4621 taaatgaagc aaaatgtgat gttgcaacaa tcggtaataa tgaagggatg accatttcac
     4681 acgaagcttt aaatcacctt tacgacgaag caaaatttat agtgacatgt agcaatgtta
     4741 tagatgaatc aggtcattta ccaaataata tcgtttcttc ttatattaag gatatagacg
     4801 gtgtgaaaat actattcgtt gcagcgacag caccttttac cccattttat cgtgcactag
     4861 attggattgt taccgatcca cttgattcta taaaagaaga aattgaactt caacgaggta
     4921 aatttgatgt attaatcgtg ctaagtcatt gtggcatttt cttcgatgaa acattatgcc
     4981 aagaattgcc tgaaattgat gtcatttttg gtagtcatac gcatcattat tttgaacatg
     5041 gtgaaattaa taatggtgta ctgatggcgg cagctggaaa gtatggtaat tatcttggag
     5101 aggttaattt aacttttgag gcacataaag tagtacataa aactgcaaag attattcctt
     5161 tagaaacatt acctgaagtt aaaacttcat ttgaagaaga aggaaaaacg ctaatgtcta
     5221 atccggtaat tcaacatcca gtagtgctta agcgtagtat gaaccacata actgaagctg
     5281 catacttatt agctcaaagt gtttgtgagt atacacatgc acaatgtgcc atcatcaatg
     5341 ccggcttact cgttaaagat attgtaaaag atgaagtgac agaatatgac attcatcaaa
     5401 tgttaccgca tccgattaat atggtaaggg ttagactttc tggtgtgaaa ttaaaagaga
     5461 ttatagctaa aagtaataaa caagaatata tgtatgaaca tgcacaaggt ttgggtttca
     5521 gagggaatat atttggagga tatattcttt ataatttagg atacattcat tctacagggc
     5581 gttactatct gaatg
//
LOCUS       DAPIDM010000153         5582 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000153 DAPIDM010000000
VERSION     DAPIDM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.153"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..359)
                     /locus_tag="RJE42_002360"
     CDS             complement(<1..359)
                     /locus_tag="RJE42_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDV5877754.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESSKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDA"
     gene            complement(604..2355)
                     /gene="srrB"
                     /locus_tag="RJE42_002361"
     CDS             complement(604..2355)
                     /gene="srrB"
                     /locus_tag="RJE42_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDV5877755.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSSASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEIQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(2336..3061)
                     /gene="srrA"
                     /locus_tag="RJE42_002362"
     CDS             complement(2336..3061)
                     /gene="srrA"
                     /locus_tag="RJE42_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDV5877756.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(3194..3931)
                     /locus_tag="RJE42_002363"
     CDS             complement(3194..3931)
                     /locus_tag="RJE42_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDV5877757.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3924..4466)
                     /gene="scpB"
                     /locus_tag="RJE42_002364"
     CDS             complement(3924..4466)
                     /gene="scpB"
                     /locus_tag="RJE42_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDV5877758.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDISKDQLVELIENYSS
                     HGLIIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISSIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(4459..5190)
                     /locus_tag="RJE42_002365"
     CDS             complement(4459..5190)
                     /locus_tag="RJE42_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDV5877759.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            5282..>5582
                     /locus_tag="RJE42_002366"
     CDS             5282..>5582
                     /locus_tag="RJE42_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDV5877760.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKL"
BASE COUNT         1698 a         1049 c          757 g         2078 t
ORIGIN      
        1 gcatctttgt acaaaggtag acttgcatta aatacaatgc catgatcttc acaatgcaca
       61 taaacttcta caccatcatc tttacctaca acatttgtag ctttaacttt tagtccaaag
      121 ttatctttaa agaattgttc acctactttt tcaaattctt tacgatgctt cttcgcaaat
      181 tcaatcgcat ctttttctgc aggcggttgg aagccttggc ctacatattt tgaagcttcc
      241 atttcttcag gtactgattt tgtttctgta ttcgtgtctt tacttgattc attttccatc
      301 gtggaacatc cccctaaaat taatgtcgta gctaaaactg atccaatgaa ttttttcata
      361 tgacacctcg aataatttaa ttattacaat atatctatat tttaccctat ttttataatc
      421 aaataaataa ctttatcaaa ctttatattt ttgtataaat tagtaatata tttaatagtt
      481 gatattcgca aaaaccctaa gtcctaatcc acatacaaaa ttccccacat caatcaaaaa
      541 acgcatgcta taaattagag tctctaatca ataacatgcg ttctgaaata actatattca
      601 gttttattcc ggctttggta gtttgataat aaacgttgtg ccttttccta attcgctttt
      661 aacatcaatg gaaccaccat gctcttcgat aatcatttta caaatgaaca aacctaaacc
      721 ggtaccttgt ttacctcgcg ttctcgctgc atcaacttta taaaaacgat caaatacttg
      781 ttgtaaatgt tctggtgcaa tgcctgtgcc tgtatcttta atgtataaaa tatcttcgct
      841 ttcattttca tcacaagtaa ttgcaatttc atcaccaggt ttcgtataac gtgatgcatt
      901 atcaattaag ttcgttagta cttggtccat tcgatccata tcataactcc aaacacgctt
      961 cttacaataa ttaaaagtca tatttagacc taaatcatca gcttgttggc gatacttaat
     1021 tttcatctta tctagtaacg ctgcaatagg ctgaatttct ttatttacgg ataacccttc
     1081 agcatccatg cgagcgacat ttaacaattc attaactaaa cgatttaaac gtttcgattc
     1141 atcaaggaca atggcaagcg attcttttat ttcatccggt tctgtaacaa taccatctac
     1201 aattgattca gtatagcctt gaagtaatga tatcggtgta cgtaattcat gtgatacatt
     1261 agcaatgaaa tctttcttca tttgatctag attgtgctca ttagtcatat cacgaactgt
     1321 cacaacgacg ccacttttac ctccctgttc aatcttgtcg atatagcttg tggtcacaac
     1381 aaagaatcgt gcattcattt ctaaatcacg catttcagtt tgttttgatt taaaagtatc
     1441 ttcaatttgt cttaataaga aagctttggc atcttcatca atattgtcca taatatcatt
     1501 cgccatctta ttcgataaga taatttgtcg actctcatta atacctagga caccttctac
     1561 catagagtta attaagctgt ctctaatatt tttagatgtg gataatgcgt cgacatgctc
     1621 ttcgatttct gtactcatct gattaaatgc ctgcgataat tgaccaattt catctttcgt
     1681 tgtgacagaa ggtttataag agtaatcccc ttcagataca cgtgtagctt ggtctcttaa
     1741 gcgtcttaaa ggttttgtaa tccttgacga taagaaaaac gcaaagactg ttgtaattgt
     1801 taagaaaata acagccgtaa ttatagtgat aatcgtaata gcattatttg tatcttcgat
     1861 tgatttcaag tctttatata taaagactcc actatatttg ctatgactat tcttctgtgc
     1921 ttttgttgga tagcctaaca aaatatatgt ttgagatgag cccttttctt taatcgttac
     1981 atttcgagta acagatttac ctttatcaaa cacatcgtca aaatggtcgt tgttgactac
     2041 ttcattcaac atttgctttt taatattaga aagtgaagct gaagattgac gatgtttatt
     2101 atttataatc attaacccac cagggttttc aattaatgtt tgactatatt tgattgcttc
     2161 ttctttattg tgcgattgtt cgaccagtga actaattcgt ctagcatctt ctcttatggc
     2221 attttcggtt tcttgtgtga aatagtattg cataaaagta attaacgcaa tacttaataa
     2281 aattaaaact gtcgtcacta ttaaaataat agttaaccac agtttaatta cgacactatt
     2341 tagccggctc atcattagat ttaacctcaa atttataccc aacgccccag actgtttgaa
     2401 tcatatgcgc agcttcgcta gacacacgat tcaacttttc tctaagtcgt ttaacatgag
     2461 tatcaactgt tcttaaatca ccatagaatt cataatgcca aacttctttt aataattgtt
     2521 cacggtcaaa tactttattt ggtgttttag ctaaatatat taataattcg tactctttag
     2581 gagtcaaatt aacttcttga ttatcagcaa gtacgcgatg tgcatcatta tctatttcta
     2641 aatgtttaaa ttcaatcaca tcacgtgcgt gaggttcgct ttgttctaca gttgtagatt
     2701 gcgttcttct tagaagtgct ttaactctta agactacttc tcttggtgaa aatggtttga
     2761 cgatataatc atctgcacca gattcaaaac cttcaacacg gtttgtttct tcacctttag
     2821 cagtcaacat aataatcggt gtttgtttat gttcacgcaa tttagttgcc acctggatac
     2881 catccatttc aggcaacatt aaatctagta gtatgcaagc ataattattc tccattgcaa
     2941 gttcataagc ctcttggcca ttacttgctt catggatttc aaaagattct ctttctaaat
     3001 acattttaag taatcttctg attctatcct catcatctac gataagtatt tcgttcgaca
     3061 tacaggtcat acctcccaca catgcttttc tttacaaaag tattatatca caggaaagcg
     3121 catacagtta ttaaaaacct aaaattttgt gaaaaaattc tcaaactttg tgataaatac
     3181 atattttatt taattatttc ccatgttctg ctaaatgacg catcactttt acttcatgag
     3241 gcgtcaatac acgtccttca ccagcattca aaccgacaac atttaaaggc ccatattcaa
     3301 tacgagacag tttcgtcact tgatgaccaa aatgttcaaa cattcttctg acttgacgat
     3361 tacgaccttc tgttattgta atttcaacca atgttgtgtt tttatcttta tcttgtttct
     3421 taactttcac ttcagccggt tgcgtcatac catcttctaa ttcaatacct ttttctagcg
     3481 ctttcacttc ttctctcatt aagtaacctt ttaattttgc tacatatttc ttcttaattt
     3541 gatatcttgg atgtgtcatt aaattagtaa attcaccatc atttgtaagt aataacaatc
     3601 cagaagtatc atagtctaga cgaccaacag gataaatacg tgcttcgata tctttaaagt
     3661 aatctgtaac cactgtacgt cctctatcat cagatacact tgttatcact tgagttggtt
     3721 tatggaataa aatgtaaatt ttgtcttcta gttctatttt aataccttca acttcaatcg
     3781 tatctgaagg cttcactttt gttcctaatt cagtgacagt tgtaccattc actttcactt
     3841 ttccttcaga aattaaagtt tctgccttac gtcttgatgt ataaccactg ttcgctatac
     3901 gcttttgtaa tctctctaat tctttagtca ttattttctc ctttttgatt gactagattg
     3961 ctgaaaaaag catccatttc ttcatcgtct tcttcagttg ttggcaaatc ttctatactt
     4021 gaaataccaa atacatttaa aaataaatca gtagtgatta attgttggct acgctgttca
     4081 ttgaccactt tagcctcaac tagtcctttg gcaatcaatg ttttaactgc accatctgaa
     4141 ttgatactac gaattaattc aatatcactt cttgataatg gttggttata agcaataatt
     4201 gatagtactt ccattgctgc ttgtgataat ttcatttgtg acttttgttc aattaattgt
     4261 tcaatgtacg ttgccgcttc ttttttagtc gttaaaacat acgtcgttcc aaatcgttgt
     4321 attattaatc catgtgatga ataattttca attaattcta cgagttggtc tttcgatata
     4381 tctaaaattt ctaatagctg tttttcatct aaaccttcat cgccagctgt aaataatagc
     4441 gactctaata taccgtgatt atccaaaatg gtagttcact cctctaataa tgttaatatc
     4501 ttcaaaatta cgttgttgct caatattaat tattcctgcc tttgacatct ctaaaatagc
     4561 taaaaagtga gtgactactt gttcaattgg ctcagaaaac gtaaacagac taaagaagtt
     4621 aaaatgatct ttatctttca atctcgatgt cacttgttct gtagcttgtt gaatggtaaa
     4681 tgtctctttt cggatttcaa cagatttagg tgtatttaac tcaactctat ttttaactct
     4741 ttgataagct acaattaatt cagttaagtc aatcgtatga tttggatccc aagattcatc
     4801 tgtttccaaa tgagataaat ctgtcggtct ttttgtaaaa taaaaatctc tttcttcttt
     4861 catgtcattt aaaatagcag tatattcttt ataattttga tattctatta aacgcccaac
     4921 taaatcttca cgtgggtcat catcaacatc catatctgat gttgattgtg gtaaaagcat
     4981 cttactttta atcattaaga gttctgacgc taatactagg tattcacttg caatattaat
     5041 ttcaagctgt ttcattgcat gaacgtactg catatactgc tctgttaatg cttgcatagg
     5101 aatatcataa atatctattt caaatttttg gataagatgc agtaataaat ctaatggtcc
     5161 attgaaagca tctaatttaa cttcatacat aatatctacc tcgtattgcg tcatatgtgc
     5221 ataattctct aaaatattat agcatgaaaa taacttactt ttaaattggg gtgataacaa
     5281 aatgcaacag gcactgataa atttctatta tcaatttcat actaagcaac attatttcct
     5341 atgtcatgac attttagaag atgcatggaa agctgaaaat aactacagta agcaagatgc
     5401 agttgttagt ttaatcttgt ttgcaactgc ttgttaccat tatcgccgca ataatttaaa
     5461 aggcgcctat aagtctttta ataaatctaa agaaataata caaaatgcta aagatagaga
     5521 tgcgttatac cttaatttaa atgattatca gttgttaatt gagcaacaga tagcaaagtt
     5581 ga
//
LOCUS       DAPIDM010000154         5410 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000154 DAPIDM010000000
VERSION     DAPIDM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.154"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(191..460)
                     /gene="rpsO"
                     /locus_tag="RJE42_002367"
     CDS             complement(191..460)
                     /gene="rpsO"
                     /locus_tag="RJE42_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV5877761.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            complement(575..1546)
                     /gene="ribF"
                     /locus_tag="RJE42_002368"
     CDS             complement(575..1546)
                     /gene="ribF"
                     /locus_tag="RJE42_002368"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDV5877762.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            complement(1561..2478)
                     /gene="truB"
                     /locus_tag="RJE42_002369"
     CDS             complement(1561..2478)
                     /gene="truB"
                     /locus_tag="RJE42_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV5877763.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQMGDTLEMKGVHSADFNNDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            complement(2648..2998)
                     /gene="rbfA"
                     /locus_tag="RJE42_002370"
     CDS             complement(2648..2998)
                     /gene="rbfA"
                     /locus_tag="RJE42_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV5877764.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            complement(3384..>5410)
                     /gene="infB"
                     /locus_tag="RJE42_002371"
     CDS             complement(3384..>5410)
                     /gene="infB"
                     /locus_tag="RJE42_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV5877765.1"
                     /translation="SNHMQALEDDQIKALDKKFKKEQKNDNKQSTQNNHQKSNNQNQN
                     KGQQKDNKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPKEIPSKVTYQEGIT
                     VGEFADKLNVESSEIIKKLFLLGIVANINQSLNQETIELIADDYGVEVEEEVVINEED
                     LSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAY
                     QIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEAINHAKEA
                     EVPIIVAVNKIDKPTSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGIDDLLEM
                     IGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSASLLVQNGTLNVGDAIVVGNTYGR
                     IRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASIV
                     QQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRII
                     HTAVGAINESDVTLANASNGIIIGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIE
                     SAMKGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKITRNAGVRIIRDGIVQ
                     YEGELDTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR"
BASE COUNT         1622 a         1023 c          722 g         2043 t
ORIGIN      
        1 catactttat ttttaatatc ttgattatta ctctaggtgt acttatatca tctttatctt
       61 attgtaacct tattattcac tcaatattag cttaacatat aataaatgtt aatatttgta
      121 aaaagctttt ttaatataaa ttaaggttgg aacataaata tgccccaacc tcaaagactt
      181 tatattaaga ttaacgacgg ataccaagtg atttaattaa ttcacggtaa cgttgaatat
      241 ctttactacg taagtagttt aataaatgtc tacgacgacc taccattttt aataatccac
      301 gacgtgaatg gtggtctttt ttgtgtgtac gtaagtgttc gtttactgcg ttgatttctg
      361 cagtaagtac agcgatttgt acttctggtg aaccagtatc agtttcgtgt acacggtatt
      421 ctttaatgat ttcgttttta cgttcttgtg aaattgccat aatcaatttc ctccttaaaa
      481 tgtttgaata ctccttaatc cgagttaggc gttggagatt cgacctacta agaaaaaggt
      541 gtggtataga ttgtttatat aactatacgc aactctaaat attataagct acttcatcac
      601 caaaatcaac tgctaataaa tatttagcac gcgatttatc atcgttcatt tgtttaacta
      661 atgggtcgat accatcaaat ttaatctcag gacgtaagaa atgatgccaa ttcacggtca
      721 ctcgttcacc ataaatatta tcctcaaagt caaagatatt cacttcgatg acaacttctg
      781 ctttgttagg atcatgaaat gttggcttta cacctatgtt agctacccct cgatataatt
      841 tattttcagt gccgatttca atactaacgg cataaacacc tttacgaggt aacaaataat
      901 catcactagg ttgaatgtta gctgttggga agccaatagt tcttcccctt ttttcacctt
      961 gcactacagt gcctttaata gaatatatat agcctaaagc atcattcgct ttttgcaact
     1021 caccattgat taaatcttga cgaatagaag ttgtagaaat tttttcattt tcaatttctt
     1081 gtttactcac aattgtcgta ttaaacgcat catattcttg aagaacagtc atattacctt
     1141 ttccaaattt accaaaagta aaatcaaaac cagcaatgac ttcttttaca ttatttttaa
     1201 ttatataatt ttcaacaaaa tcttcaacgc tcacattagc aaacctagat gaaaaattaa
     1261 ccactataca ataatcaata tcatgttggc taattttttc gattttatct gaaagtggtg
     1321 ttaaatacgt tgttcgtttt cttttaggat tcaacacgac agacggatgc ggatcaaatg
     1381 tcatcaccgc tttttttaaa ctgcgtgcct cagctatttc gtttaatata tcaaagactt
     1441 tgtcatgacc tttatgcatg ccatcgaaaa atccgaatgc cattgcaaca tcctctgtaa
     1501 tatactgttt agattgtata ggatgtgtca cttctatgac tttcataaat tctatctcct
     1561 ttaattaaag acttttttag gtttaatttc tgattctttt gtagggtgta ccatataaat
     1621 tgctaatact ttttctgaat catcatcaat aaatacaatt tggtctttaa ttttattatc
     1681 aaattcattt ttattaaatt tctgcccatt taaaatacgt ttttttatgt gcgaatcttt
     1741 aattttaatg cttggcaaac cctttaatcc atattctaaa ggaaacaatt tattttgcaa
     1801 tgaatcctgc tcatgaagtt cttttatttg ttctaatgta aggctatctt tcaacacaaa
     1861 tccaccagac tcgattcgcg ttaatttcga catatgtgcc ggaaagccta atttcacacc
     1921 aatatcagtt gctagtgttc taatatatgt acctttacca cagatgacgc gtattttaaa
     1981 atgacactca ttttctttaa aatctaattc agatatacgc ccaatgtctt taatattaac
     2041 tttacgcttt ggtctttcaa ctgtttcatt attacgcgca tattcatata attttttacc
     2101 attcaccttg actgatgaat acattggcgg aatttgttca atgatacctt taaaactttc
     2161 taacaatcgg tcaatatcgt cattgttgaa atcagctgaa tgtacaccct tcatttccaa
     2221 tgtatcaccc atttgatctt cagttgttgt acttctgcct atcgataccg ttgcctcata
     2281 agctttgccc atatccataa cataatcact aactctcgtt gcattaccta tacataccgg
     2341 taacacgcct gcaacttcag gatcaagcgt accagtgtgc cctatttttt tagtctttaa
     2401 aattttacgc aatttgaata cgacgtcatg acttgttaaa ccacgttctt tatatactgg
     2461 taatataccg ttatacatgt tgtcaccttc ttttttgaaa atatatttta atagcgtatt
     2521 aactgaattt attcataaaa aaactggaaa atgttaacta tttctacaca tctatgtatt
     2581 aactgtttaa tgttaatata ccgatattaa gaaattatca aacattttcc agatacctaa
     2641 cactaaatta tctatcttgt ttgtgtaaat cttgaatcat tcgttcaatt ttattaccat
     2701 attcgattga ttgatcatat tcatacatta attccggcat aatacgtaat cgcattctag
     2761 aacctaattc agacttaatg aagccttttg ctttatcaag tgctttaaat gtattttcta
     2821 cttctttatc gttacctaat acagttaaaa atactttagc ctgcgataaa tcatttgtta
     2881 aaacaacatc tgtaattgta ataaaaccaa ctcgaggatc tttgacttta ttgttgatga
     2941 tatccattaa ttccttcttc atttgttcac caacacgctc tgctctcata ctgctcattc
     3001 ttgtcacctc tttatcttta agttcaattg ttatttgcat tttttagtgt aacaatgctt
     3061 aaacaattat tttagctcac acgttatttt aataaattaa gcctataaaa tcaaataaag
     3121 tttgccgtaa gataggcatt tcaacagaaa attttccaat aatagtcgac atgatatttt
     3181 gtcattaaaa atcattctag gccaccacaa aattttatac aaaaacatgt ctatataacg
     3241 taacttttaa taggtaatct attctaataa aaaaccaaaa gctagtatca aatatgctcc
     3301 ctataaagta gacacttaaa aagtgaaata tttataggga gcatatcaac cttaactata
     3361 cttatagctt gtaatttatt taattaacgc ttaatttcaa ccatttcaaa tgcttcgata
     3421 acatcgcctt ctttaaggtc attgtagttt tcaattgtaa taccacattc ataacctttt
     3481 gcaacttcct tagcatcatc tttgaaacgt ttaagtgtat ctaattcgcc ttcatattga
     3541 acaataccat cacgaataat acgtacacca gcatttcgcg taattttacc ttcagtaaca
     3601 taacatccag caatagtacc aactttagaa actttgaatg tttgacgaac ttcagcttgc
     3661 ccgataactt gttcttcaaa ttctggatca agtaagcctt tcatcgctga ttcaatttct
     3721 tcgataacat tataaataac tctgtgtaaa cgcatatcaa cattttcagc ttctgcagca
     3781 cgttttgcac cactgtctgg acgaacattg aaaccaatga taataccatt tgaggcatta
     3841 gcaagtgtca cgtctgactc gttaatcgca ccaaccgctg tatgaatgat acgaacattt
     3901 acgccttcaa catcaatttt cattaatgat gcagctaaag cttcaacaga accttgaaca
     3961 tcacctttaa taataacgtt taaatctttc atttcacctt gtttcatttg ttcaaacagg
     4021 ttatctaatg aaacattttt actttcttga cgttgttgta caatgctagc ttcgtgtctt
     4081 gattcaccaa tacgacgagc ttgtttttca tcactaaata caacaaagcg atccccagct
     4141 tgtggcacat cattaatacc tgtaatttca acaggcgttg atggaccagc cgttttgatt
     4201 ctttgaccta agtcattaac cattgcacgg atacggccgt aagtattacc cactacaatc
     4261 gcatcaccaa catttaatgt accgttttgt actaataatg atgcagaagg accacgtgat
     4321 ttatctaatt cagcttctat aactgtacca acagcacggt ttttaggatt tgctttaagt
     4381 tcttgaactt ctgcaactaa tcctatcatt tctaataaat cgtcgatacc atcaccactt
     4441 aatgcagaaa gtggaacgaa aattgtttcg ccgccccaat cttcaggaat taaaccatat
     4501 tcagttaatt cttgcataac tcgatcagga tttgaagttg gtttatcaat tttatttact
     4561 gcaacaataa ttggtacttc tgcttcttta gcatggttaa ttgcttcaat tgtttgtggc
     4621 ataacaccat cgtcagctgc tactactaaa attgtaatat ctgttacttg tgcaccacgc
     4681 gcacgcatcg ttgtaaatgc agcatgtcca ggtgtatcta agaaagtgat ttttttgcca
     4741 tcgttttcaa tttgatatgc accaatatgt tgagtgattc cgcctgcttc acctgctgta
     4801 acttttgtat gacgaattga atctaataaa gtagttttac catggtcaac atgtcccata
     4861 attgttacaa ctgctggtct ctcaattgct tctggatcat ctttttcgtc ttcgaagtag
     4921 attgataaat cttcttcatt aatcacaact tcttcttcaa cctcaacgcc ataatcatcg
     4981 gcaattaatt cgattgtttc ttgatttaat gattgattga tattagcaac aataccaagt
     5041 aagaataatt ttttgataat ttctgatgat tcaacattta atttatccgc aaattcgcct
     5101 actgtaatac cttcttgata tgtcactttt gatggtattt cttttggagc agctggttga
     5161 ttttgtggtt tattattctt gttatttttc ttattatttc tattattctt tttattgcct
     5221 ttgttgcctt tattattttg ttgttgattc tttttgttat ctttttgttg ccctttattt
     5281 tggttttgat tgtttgattt ttggtgatta ttttgagtgc tttgtttatt gtcgttcttt
     5341 tgttcttttt tgaacttttt atctaatgct ttaatttggt catcttccaa agcttgcata
     5401 tgatttgaaa
//
LOCUS       DAPIDM010000155         5385 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000155 DAPIDM010000000
VERSION     DAPIDM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.155"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(214..2973)
                     /gene="clfA"
                     /locus_tag="RJE42_002372"
     CDS             complement(214..2973)
                     /gene="clfA"
                     /locus_tag="RJE42_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDV5877766.1"
                     /translation="MNMKKQEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSM
                     TQTENTRNESKSNDPSSVNAAPKTDNTNVSDSNTTTNTNSDETNVAQNPAQQETTQSA
                     STNATTEETPVTGEVTTTATNQANTPATTQSSNTNAEESVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEVTPSNNESAPQSTDASNKDVVNQTVNTSASRMRAFSL
                     AAADAPAVGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVQGDTFKIT
                     VPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDTKNDVKATLTVP
                     AYIDPENVTKTGNVTLTTGIGNTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTY
                     RQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVNP
                     ENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTLY
                     GYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGSD
                     SGSDSGSDSNSDSGSDSGSDSTSDSDSDSASDSDSASDSDSASDSDSTSDSDSTSDSD
                     STSDSDSTSDSDSVSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSESESDSDSDSDSESDSDSDSDSESESDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDNDSDSDSDSD
                     SASDSDSGSDSDSSSDSDSDSASDSDSDSDSTSDTGSDNDSDSESDSNSDSESGSNNN
                     VVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKE
                     NKDKK"
     gene            complement(3237..3767)
                     /locus_tag="RJE42_002373"
     CDS             complement(3237..3767)
                     /locus_tag="RJE42_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV5877767.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPKYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVKSL
                     AGKTGTINYTSAFEKI"
     gene            complement(3906..4427)
                     /locus_tag="RJE42_002374"
     CDS             complement(3906..4427)
                     /locus_tag="RJE42_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877768.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREGGQLNIMNSATDEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     NYNNKEIYDLQFI"
     gene            complement(4435..4590)
                     /locus_tag="RJE42_002375"
     CDS             complement(4435..4590)
                     /locus_tag="RJE42_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877769.1"
                     /translation="MMVKKLLKTIKQNFLYEQSNITFKMFLFGYKRLSKMIFQFEINH
                     INFLREG"
     gene            5036..>5385
                     /locus_tag="RJE42_002376"
     CDS             5036..>5385
                     /locus_tag="RJE42_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDV5877770.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINIT"
BASE COUNT         1641 a          984 c          792 g         1968 t
ORIGIN      
        1 ttctccttaa ttttccctaa ttaacaacct accttacacc ctattttttc gccaagccaa
       61 attatataac atctaccttt ttaaaataat taactgttga tgaattaatt attaattata
      121 ttcaaacaat atatttggaa aatccaagta aaaaagccac cttttaaggt ggcttcttca
      181 tgaatcatat gattaattta atatcattat tacttatttc ttatctttat tttctttttt
      241 tcttctgaaa agtagtaatg aacctaatga tgctaataat ccccaaatta gtgacgtatt
      301 cgcttcatct tcagaacctg tatctggtaa tggctcttta ctatctttag cctcattttt
      361 attagaagca ttagtaccat tttttggtga attaggcgga actacattat tgttagaacc
      421 tgactcagaa tcgctatttg aatcactttc tgagtcggag tcgttgtctg atcctgtgtc
      481 actcgttgaa tccgagtcac tatctgaatc agaggcactg tctgagtcgg aatcacttga
      541 tgaatcggag tcactacctg agtcggaatc actcgctgaa tccgaatcgc tgtctgagtc
      601 tgagtcatta tctgagtctg aatcactgtc tgagtctgag tcactgtctg aatcggaatc
      661 gctatctgag tctgaggcac tgtctgagtc ggaatcgcta tctgagtctg agtcactgtc
      721 tgagtctgag tcactgtctg aatcggaatc gctgtctgaa tcggagtcac tatctgaatc
      781 tgagtcactg tcggaatctg agtcgctttc tgattctgag tcgctgtctg agtcgctatc
      841 cgattctgaa tcgctgtctg aatctgaatc gctttctgat tctgagtcac tgtctgagtc
      901 ggaatcgcta tctgaatccg agtcactgtc ggaatctgaa tcgctatctg aatctgaatc
      961 gctgtctgag tctgagtcac tatctgaatc tgaatcgctg tctgagtctg agtctgagtc
     1021 actatctgaa tctgaatcgc ttgctgaatc tgaatcactc actgaatctg aatcactcgt
     1081 tgaatctgaa tcactcgttg aatctgaatc actcgttgaa tctgaatcac tcgttgaatc
     1141 tgaatcactc gctgaatctg aatcgctcgc tgaatctgaa tcgctcgctg aatctgaatc
     1201 actatctgat gtagaatcac tacccgaatc tgaaccgcta tctgaattag aatcgctgcc
     1261 agaatctgaa ccactatctg aacctgggtc agaatctgaa tcctctggaa ttggttcaat
     1321 ttcacccggc tcatcaggtt gttcaggaac cacaggttta tcgataccgt caccagaacc
     1381 tgatccgtta ttaaatgcta cttcgttgtc ccatgacata gagcgccaaa ttatatttga
     1441 gttatatcca tataaagttg aacgtaaagc taaatcacct ttgctattcg gatcaatatg
     1501 accattaaca actacaatat acggtgttgt aatttgatca tcaggcgtat taaactctac
     1561 tttatattga tttggatttg ggaatgtaat atttacacta ttagtgacat cctcaaagtt
     1621 ttctggattc acaaagtaac tttcagataa atcagctgca ttatctactt tatatacttt
     1681 aatacttgta ttttgctgat ctattaatgc attactatcc gtatttggtt ttaaattacc
     1741 tgttaaaacc ggtgcaataa cgttatctcc acttggattg acataaattg tctgacgata
     1801 cgtattattt gttttatcga tttggtcaat tgtaccttta atagataagt tataaaactt
     1861 accatatttt tcataatcta ctaatactgt tttgtttgct gttgtatttc ctatgccagt
     1921 tgtcaatgtc acattacctg ttttcgttac attttctgga tcaatgtatg caggcacagt
     1981 tagtgttgct ttaacatcat ttttagtatc aacatagtct gtaaatgtat aaataacatt
     2041 accatcacta tcgattacac catttgccaa tacttgatct ccggccataa ttggtggcac
     2101 tttagcagtt gaagttacac catttaagtt taattctttg ggcacagtta ttttgaatgt
     2161 gtcaccttga accgcagaat ttggtactga gaaaccataa ttcagtttga cataacctgc
     2221 ttggtgcgga tacacagtcg taccagagtc aataccaact gtcacattcg tcaactgatt
     2281 cgtaatatct gtgccaacag ccggtgcatc tgcagccgct aaactaaatg ctctcattct
     2341 agacgcactt gtattaaccg tttgattaac tacatcttta ttacttgcat ctgtactctg
     2401 tggagctgat tcattgtttg aaggtgttac ttcagttgaa gtgtcttgcg ttgttgaaac
     2461 attttccgca tttgtagaat tttgaggtga atttacagat gatactgtat tagtatcatt
     2521 agaagtcgtt tcattacttg tttgattcac tgattcttcc gcatttgtat tgcttgattg
     2581 agttgttgcc ggtgtattag cttgattcgt tgccgtagta gtaacttcac cagttaccgg
     2641 tgtttcttct gtagttgcat ttgttgatgc tgattgtgtc gtttcctgtt gtgctggatt
     2701 ttgcgctaca ttcgtttcgt cactattagt gtttgtcgtt gtattagaat cactcacgtt
     2761 tgtgttgtct gttttaggtg cagcattaac gctacttgga tcattacttt tgctctcatt
     2821 tctcgtattt tccgtttgtg tcatactatt ttcacttgca tctgcttctt tactgctgag
     2881 tagtccaaaa ccgattaacg tacctacaag cactgaagcc acgccaatcg attttttacg
     2941 aattgcgtgt ttttcttgtt tcttcatatt cattttattc cctcttttta aaaagtcatt
     3001 ttatattaac tgtataccct tttaatatat atttaatctc tattaatgca attatacacc
     3061 agaattgcat aatggcaatt aatttgtttt agaattttat taactacaat aaatatttac
     3121 aacaaaaagc ttttcatttt ctctgttttg aaaattgcaa acgacaacgc acgatttcat
     3181 tgcaaacaat ttgtattctt aatatgaact ctgcctaatg taatcctaac ttgaaatcat
     3241 attttttcaa aagcagatgt gtaatttatg gtacctgttt ttcccgctaa acttttcact
     3301 tttaattctt taattaaaaa cgcttcatct ggtataccat catatggtgg ataaatgtcg
     3361 tacatacttg cgcgttgata tcctagattg gcataatact tcggccatcc taatacactg
     3421 ataaaattat atccttttaa tattgcttct cgttctaatg cttgaatcaa tcgcttccca
     3481 atacctttat tttgatgatg aatatcaaca gatacaggtg ctaacactaa tccaatttcc
     3541 cgttgtgcct cgttatcaag ataaacttca cttagtaaac cgtgccctac aacttcattt
     3601 tgaagaacag ctactaattc taagttattg tcatacgtat cacttagacg aatttggtct
     3661 actagttctg attcattacc ataaccatgt tcactatttt caaatgccgt tctaattaac
     3721 tggtcaactt gagcaaagtc atgttgatgt atttgtctaa tttgcatctt tgcccctcct
     3781 tttagttcta tcttatagaa aaatgcatta caacataaag taatatttta tagtaatgac
     3841 gccattattt cattaaaatt gaatgatatt tttcgcaata aacaactaca ctcttaaatc
     3901 aatttttaga taaactgcaa atcataaatt tctttattat tataattatt aaaacggtcg
     3961 atttgacctt ttaatataac ttcctgttgt ttgcgactta cattatgata ataagtcata
     4021 cgtacattca aatcagcttc ataaccatta ttctcaatcg ttctttctaa ttcttcgtac
     4081 acatttttat taattggtaa catgtaccct tctttactag gtaaaatatt cacgtatttt
     4141 ttctgtatga ctttatcatt ccccaataaa acctctattg caattttttt agcagcacca
     4201 ccacctaaat tatatagttt aatataatct ttatgatgat gctcatctgt agccgaattc
     4261 attatattaa gttgaccccc ctccctttct aataaaattt ggttaaaacc taaagctggc
     4321 agaaagctaa ttttcatttg ataaagttga actgaaactg atacaaaata aaataaagcc
     4381 ataataaatg ttccaataga tcctatagct gaaataatat taatcattaa gatatcaccc
     4441 ttcccttaag aaatttatat gattaatttc aaactgaaaa atcattttac ttaatcgttt
     4501 atatccaaaa agaaacattt taaacgttat attcgactgc tcatataaaa agttttgctt
     4561 tatagtctta agtaattttt taaccatcaa gtacgtttat tattatggca atctatttgc
     4621 tttgactaaa gaatacaata acgcttttaa gagctcccat ctttgagcat tatttccaat
     4681 ttaataactc atgttcttca atacatattc atctcataca aataagttga atttttttat
     4741 ttttataaac gtaatttctt aacaaatcaa aattttacat ttaaatcttt cgatatatta
     4801 caagtttttt aaaaaagtaa tatatttcta tcaataaaag taatataata ttagtaatta
     4861 tagttccatt aatttattgc aacaatacat agaatcatta ttacaagaat tatttaatat
     4921 catcttagaa tctcacaaaa tatcaacttt gtttaattac tcaaaacgat aagcaatttt
     4981 atatcgttac gagtttttaa aaataaattt ttgcgaaata aaggagacat gttaaatgaa
     5041 aaaagtaatg gggatattat tagcaagtac acttatctta ggtgcttgtg gacatcatca
     5101 agatagtgca aaaaaagaga gcactagtca caaaaagaaa gaaaatgaca atgaagaatt
     5161 aaatgaagaa cttaaagaat ttaaaagcaa aaaaaatatg gatataaaaa ttaaaggcga
     5221 tactattgtt agtgacaaat ttgaagctaa aataaaagaa ccgtttatca tcaatgaaaa
     5281 agatgagaaa aagaaatata tcgcttttaa aatggaaatt actgctaaaa aagacgataa
     5341 agatttaaat ccatcttcta tttctcatga ctatattaat atcac
//
LOCUS       DAPIDM010000156         5352 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000156 DAPIDM010000000
VERSION     DAPIDM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.156"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            10..1356
                     /locus_tag="RJE42_002377"
     CDS             10..1356
                     /locus_tag="RJE42_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV5877771.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKADV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAITGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            1366..2256
                     /locus_tag="RJE42_002378"
     CDS             1366..2256
                     /locus_tag="RJE42_002378"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV5877772.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            2382..3167
                     /locus_tag="RJE42_002379"
     CDS             2382..3167
                     /locus_tag="RJE42_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDV5877773.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            3209..4072
                     /locus_tag="RJE42_002380"
     CDS             3209..4072
                     /locus_tag="RJE42_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDV5877774.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            4059..4469
                     /locus_tag="RJE42_002381"
     CDS             4059..4469
                     /locus_tag="RJE42_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDV5877775.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            4745..5344
                     /locus_tag="RJE42_002382"
     CDS             4745..5344
                     /locus_tag="RJE42_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDV5877776.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
BASE COUNT         1949 a          716 c          980 g         1707 t
ORIGIN      
        1 ggccaaatca tggcaaaaca tccattcgaa caatttaatc tagaatctag tttaattgac
       61 gctgtgaaag accttaattt tgaaaaacca actgaaattc agaatcgaat tattccaaga
      121 atactaaaga gaacaaattt aattggtcaa tctcaaacgg gtacaggaaa atctcatgca
      181 tttttattac cattaatgca gttaattgat agtgaaataa aagaaccaca agcaatcgtg
      241 gttgcaccaa caagagaact tgcacaacaa ctatacgatg cagcgaacca tttaagccaa
      301 tttaaagctg acgtttcagt taaagttttt attggtggta cagatataga gaaagataga
      361 caacgttgta atgcacaacc acaattgatt ataggcaccc ctactagaat taatgactta
      421 gctataacgg gacatttaca tgtgcactta gcatcatatt tagttattga tgaagcggat
      481 cttatgattg acttaggatt aattgaagat gtagattaca tcgctgcaag attggaagat
      541 aatgcaaata ttgcggtgtt tagtgctaca attccacaac agttacaacc atttttaaat
      601 aaatatttaa gtcatccaga atatgtagct gtcgacagta aaaaacaaaa taaaaagaac
      661 atcgaattct ttttaatacc tactaaaggt gcagctaaag ttgaaaagac tttaaattta
      721 attgatatac taaatccata cttatgtatt attttctgta atagtagaga taatgcaaat
      781 gatttagcac gttcactaaa tgaagctggt attaaagttg gtatgattca tggtggttta
      841 acgccacgtg aacgtaaaca acaaatgaaa cgtatacgta atttagaatt ccaatacgtt
      901 attgccagcg atttagcatc tcgtggtatt gatattgaag gtgttagtca tgtcatcaat
      961 tttgatgtgc caaatgatat tgacttcttt acacatagag tcggccgaac aggccgtggg
     1021 aattataaag gtgtagcaat tacgctttat agtcctgatg aagaacacaa tatttcatta
     1081 atagaagatc gcggttttgt tttcaatact gttgatatta aagatggtga gttaaaagaa
     1141 gttaaagcgc acaatcagcg tcaagcaaga atgcgcaaag atgaccattt aactaatcaa
     1201 gtgaagaaca aagttcgaag taaaattaaa aacaaagtta aaccaggtta taagaagaaa
     1261 tttaaacaag aagttgaaaa aatgaaacgt caagagcgta aacaatttag taagcagcaa
     1321 aatagacaaa aacgtaagca aaacaaaaaa ggttaggtgt gttttatgtt attaggatca
     1381 catgtttcaa tgagtggtaa aaagatgtta gaaggttctg ctatagaagc gcatgaatat
     1441 ggtgaaacaa catttatgat ttatactggt gcacctcaaa acacgcgccg taaaagtatt
     1501 gaagatttaa atataactaa aggtcatgaa gtgatggaaa aatatggctt atctaatatt
     1561 gttgttcatg caccatacat cattaatatt gcaaatacaa ccaaaccaga aacgtttaat
     1621 cttggtgtag atttcttgca acaagaaatt gaaagaacgc aagctatagg cgctaaagat
     1681 attgtattac atccaggtgc acatgttgga gctggtgttg atgccggaat taataagatt
     1741 attgaaggat taaatgaagt tttaacgaat gacaataatg tacgtattgc gcttgaaacg
     1801 atggcaggta aaggtacaga gattggtcgc tcgtttgaag agttagcacg tattattgat
     1861 ggagttcata ataacgaacg tttatcagta tgttttgata catgtcatac gcatgatgct
     1921 ggatataatg tgaaagagga ctttgatggc gtattaaatg aatttgataa aatcattgga
     1981 gtcgacagaa ttaaagttgt tcatgtcaat gattctaaaa atgaccgtgg cgctcaaaaa
     2041 gaccgacatg aaaatattgg cttcggttac attggttttg atgcattaaa ttacattgtg
     2101 catcatgata gttttaaaga tattccgaaa atcttagaaa caccttatgt tggtgaagat
     2161 aagaaaaata aaaaaccgcc atataaactt gaaattgaaa tgttaaaaca acagcaattt
     2221 gacccagaat taaaaaataa ggttatgcaa caataagatg ataatgctct cttcaagaaa
     2281 tgcgataaat aaaacttgaa gggggcatat ctctttattt tgtctaattt tgagtcgtaa
     2341 acattactgt ttacatatgt taccaggagt tgtatagttg aatgcgaggt gagaatacga
     2401 tgacaccagt ctttgaattg aaaaatgtca attactacta tgatcataaa aaagtgttag
     2461 aaaatataaa cattaaaata aataaaggtg aatttttagc aattgttgga ccaaatggtg
     2521 ctggtaaatc aacattattg aagttgattc taggattatt acctttacaa agtggtgaga
     2581 tttttgttga aggtattgat tttaaaaata agaaaacatc aattaaatta agctatgtat
     2641 cacaaaaagc aaatgccttt aattcaggtt tcccagcaag tgttaaagaa gttgttttaa
     2701 gcggattaac aaagacaaaa cgtcttttcc aaacatttaa tagcaaagat aatgaaaaag
     2761 tgattaaagt actagaaaga ctgaatataa gtgatttaat tcataaaaat atagcagaat
     2821 tatcaggtgg tcaacaacaa cgtgtaatga ttgctcgagc attgatttca gaacctgcag
     2881 tattagtact tgatgaaccg acgaatggta ttgatgcaaa acatgtaagt gaattttata
     2941 atactttaga tcaattaaaa caagaaggta tcactattat cttagttact catgatattg
     3001 gtgttgtagc agatactgct actgaagtag catgtttaaa taagcatttg catttccatg
     3061 gtacaactga tgagtttaaa tcacttgatg aagttgaaat ttcaaaaatt tatggacatc
     3121 ctgtacgttt tgtcgatcat cagcataatc gagaatgttg taattaataa agatttgagt
     3181 ttatttaaaa tggaaaggcg tgtcacaatt gatagatgca ttattgaatt ttgattttat
     3241 gaggtactct ttaattagtg gtatcttgat aggttttatt gcgcctctaa tcggtgcttt
     3301 tatcgttgtt agacgactat cacttatagc tgatgctcta agtcatgtaa ctttaggtgg
     3361 tatatccttc ggtatgtttt tacttactat tatgccaaca ctagtattta ttaatccaat
     3421 gtggtttgga atcttattcg caatagtagg tgcgcttcta attgaaaaat taagaacgtc
     3481 atacactgct taccaagaaa ttgctattcc aattataatg agtgctggta tcgccttgag
     3541 tgcaatcttc atttcattag ctgatgggtt caatcaagaa attgttggat tgctatttgg
     3601 ctcaataagc gcagtgaata taagtgattt aacaacgatt attgtcattg ccatcatagt
     3661 tgttctgttt attacattgt tttataaaga attatttata ttatcatttg atgaggaata
     3721 tagtaaagtc attggcattc ctaaatggat tcaattttta tttattgtta ttgtagcgat
     3781 ggttatttct gcctcaatgc gtgttgtagg gatactgtta gtaagtgcgt taatcacgtt
     3841 accaattgca ataagtatgc gaataacaaa aagttttaag caattgatat tactaagtgt
     3901 atttttaggt gaattatcag taatattagg cttagtccta gctttctata tggacatttc
     3961 tccgggtggt gtaattgtag ttctactagt tattttactt atgattacaa tggcttatca
     4021 gaaaatgcga atgaagttta aaaagggagc taatatcaat gaatacaaat gatgctatta
     4081 aaattttaaa agagaacggt ttaaaatata cagataaacg taaagatatg ttagatattt
     4141 ttgtcgaaga agataagtat ataaacgcaa agtatataca acaagttatg gatgaaaatt
     4201 atcctggaat ttcattcgac acaatatata gaaacctgca cttatttaaa gatttaggga
     4261 ttattgaaaa tacagaactt gatggtgaaa tgaagtttag aatagcatgt acaaaccatc
     4321 atcatcatca ttttatctgt gaaaagtgtg gagatacaaa ggtaatagat tattgtccaa
     4381 tagatcagat aaaattatca ctacctggtg ttaatattca caaacacaaa cttgaagttt
     4441 atggtgtatg tgagtcttgc caagattaat ataaagaaat gagatttatg cacatttggt
     4501 cagatgtatg cataaatctc attttttaaa ttatagaaga taaaggttta tttcatactc
     4561 cgttcgtatg attttatggc aaatctgtta actttttata tacaaaatgt ttaattattt
     4621 ttgatttgag ttatattaaa tgagtagaga aaataataag tagtagaatt taagcaaatt
     4681 ctttgttgaa ccagtcactg cttgttatta tttacttaca gacattttag gaggatgatt
     4741 atttatggct tttgaattac caaaattacc atacgcattt gatgcattag aaccacattt
     4801 tgacaaagaa actatggaaa ttcaccatga cagacatcat aacacttatg ttacgaaatt
     4861 aaatgctgca gtagaaggta cagatttaga atctaaatct attgaagaaa ttgttgctaa
     4921 tttagacagt gtaccagcta acatccaaac tgctgtacgt aataatggcg gtggacattt
     4981 aaaccattca ttattctggg agttactttc accaaactca gaagaaaaag gtactgtagt
     5041 agaaaaaatt aaagaacaat ggggttcttt agaagaattt aaaaaagaat ttgctgacaa
     5101 agcagctgca cgctttggtt caggttgggc ttggttagtt gtaaacaatg gccagttaga
     5161 aattgtgact acaccaaacc aagataatcc attaactgag ggtaaaacac ctattttagg
     5221 tttagacgta tgggaacacg cttattacct aaaatatcaa aacaaacgcc ctgactacat
     5281 tggcgcattt tggaatgtag ttaactggga aaaagttgac gaattatata atgcaacaaa
     5341 ataaagtatg at
//
LOCUS       DAPIDM010000157         5234 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000157 DAPIDM010000000
VERSION     DAPIDM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.157"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(48..1403)
                     /gene="brnQ"
                     /locus_tag="RJE42_002383"
     CDS             complement(48..1403)
                     /gene="brnQ"
                     /locus_tag="RJE42_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDV5877777.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(1682..2239)
                     /locus_tag="RJE42_002384"
     CDS             complement(1682..2239)
                     /locus_tag="RJE42_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDV5877778.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIVFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRYDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(2306..3946)
                     /locus_tag="RJE42_002385"
     CDS             complement(2306..3946)
                     /locus_tag="RJE42_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDV5877779.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLASKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLKQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGENVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            4138..4239
                     /locus_tag="RJE42_002386"
     CDS             4138..4239
                     /locus_tag="RJE42_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877780.1"
                     /translation="MMLQNTITMKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(4218..>5234)
                     /locus_tag="RJE42_002387"
     CDS             complement(4218..>5234)
                     /locus_tag="RJE42_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV5877781.1"
                     /translation="GLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVG
                     LVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLD
                     ACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQ
                     VVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGD
                     GIAIKPSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLH
                     VEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQL
                     FEAK"
BASE COUNT         1710 a          887 c          928 g         1709 t
ORIGIN      
        1 gaaaaacgcc acaaaagcat acgttcaagt atacttgtgg cgttatatta tgcttgtgga
       61 attttacgat gcttaatttt ataaataatg aagccgataa tgaaaccaat caaactgaga
      121 acaacccagc ccataccaat gtctgataat ggtaaatatt tttggctgaa attaatcaaa
      181 gtttgtgaga atgatgtgct tgaaatgaac tctggactag cttttaatcc atctactaat
      241 gcagcaatca ttgtaaagaa aatggtacat tgataaataa tttttgaatg atggaatttg
      301 ctgctaaata atgttagtac aatcaaggca attgctaatg gatataagaa cattaacact
      361 gggactgagt acataataat tttagttaag ccaacattcg cgaataagaa tgagataaag
      421 ctcacaactg ttgcaatcgc taggtaattc attttaggga aaaggtgttc aaatgtttct
      481 gaaaatgccg taattaaacc gatagctgtt tttaaacaag caaccataac gatgagtgat
      541 aacaagacga taccgtagtt acctaagtag tattgcgtaa tttgcgctaa ggcaatacca
      601 ccattttcac taagtttgaa atgaccaata cttaatgtac ccataattgc tagtagggta
      661 taaatgatcc ccatcataat gatgctgata gtaccagact taattgtttc tttagcgata
      721 tcagttggat tttcgatacc taacttttta atcgttgcaa caatgatgat accaaatgcc
      781 aatgacgcta gcgcatctaa ggtattgtat ccatctaaaa agccgttaaa taaggcattt
      841 gattgatatt gtttactaat aggtgcatca gatatgccac ctaatggatg gataaaagca
      901 aataataaaa taattgctaa taatactaag aataccggat ttaaaaattt accgatatat
      961 tctaaaattc ttgatggctt tctcgcaaaa aaccatgcaa tcacaaagaa gacgaagcta
     1021 aaaataaata aatataaagt gatttgcttt ggtgataaaa atggcgaaaa tgcaatttca
     1081 aatgatgtag ttgccagtct aggtaaggcg aaaaatggtc cgataactag atataaggca
     1141 atcgtgaaaa tgtaagcata tgttttatta acacgcgatg caatttcaaa taaaccagat
     1201 gtctttgaaa tgccaatagc aatgatacct aaaaacggta agccaattgc tgtaattaaa
     1261 aatcctaagt tagcgataaa aacgttagaa ccagcagctt gacccaagtg tattgggaag
     1321 ataagattgc cggcaccaaa gaataaacca aataacatag aacctataaa catgttttct
     1381 ttaaatgtta gtttcttctt catatatgta tttgttctcc tttttagaac ttataaaaat
     1441 ctacttaatt aagataatat caaaaattaa acctaaagtg atacataatt ttgcaataaa
     1501 tactaaattt tttgaaaatt ccggaataaa tactgttttt tatgtagtag gggcgtaagt
     1561 taagaacgat ttaacagata taggctaagt ttatagatag gaggttagac aacccgatgt
     1621 attaatactt tgtgaaataa agatcccccc ttcaacatga tcgaaggggg gaagtatatg
     1681 tttagttcaa tatatttaga aagtcgtttg tagttattgt ttgtcccatc aatgggaata
     1741 cattatctat tggaaattga tgtagcgttt cgttttgtgc actcatcatg tcagtaacaa
     1801 aaaactgatt gtagtttaat tgataggcat ctcgcgctgt cgtatctacg ccaatatgcg
     1861 ttgcgacacc accaagaaca atcgtatcaa ttcctcgacg tcgcaattgt aagtccaaat
     1921 ctgttcctac aaatgcacta aaatgtcgtt tgtctatgac aaaatcgtca tatctcttgt
     1981 ctaataaatg atggaaacga ctgtagtcgt cgccttcttt tggtggtaat gagatcattg
     2041 catttggttg caatacatct ttaccatcat agaaattcac acgaacgaat acgataaagc
     2101 cattgttttt tctaaaaaca tctattaatt tattagcgtt ttgaacgaca ttttcagctg
     2161 tatgtggcgc ataatccatc ttaagtatac cttcttgcag gtcgattaac actaaagcgg
     2221 ttttattaaa attaatcatc ttatatcaga gtccttttca tttaataata tatctgttaa
     2281 agcttatacc ctgtataaga tgaaattatt tattttgttg cgaaaaagct ttagcgatat
     2341 cgatcagttt ctttggtgca tcttcgacag acattttgac ttcgacaaaa tgcatcacat
     2401 cgggatgacc attaattgca ttaaacgtgt cttgtaaatc ttttgatgat tcaacgtcat
     2461 gaatttcaac attttcacca ccaaatacag ctggtaaagc tttataatcc cacatgtgaa
     2521 tttcattata aggttcatac atgccgtgaa taagtcgttc taccgtatag ccgtcattat
     2581 taatcacaaa taataccggt ttaatatgct gtctaatcat agttgaaata gcttgaacag
     2641 ttagttgcaa tgagccatca ccaattaata ataagttacg acgatctttg tctgctaatt
     2701 gtgaacctaa tgttgcaggt aatgtatagc cgatagaacc ccataacggt tgccctataa
     2761 aagtattgtt tttgtataat gctaaatcat aagcaccaaa gaatgatgta ccttgatcag
     2821 caataatgac atcatttggt tttaagaaat tttgcatcat tttaaaataa gtttgttgtt
     2881 ttaatggttc tgtgccaaca gtataatcgg gtgatgttgg acgatgatac gcagggaacg
     2941 ttgcgttatt cgtatatgaa atattggata actgttttaa caatgatggc agagatattt
     3001 catcatttgt aacatcgtca attttgatat tgtgatgatt taacataacg acatcatcga
     3061 tattgaattg gtatgaaaaa cctgctgttg ctgaatctgt taatttggct ccaatattta
     3121 aaattaaatc gctgttgtcc acataatctc gtattttatc ttcggcaatt ttcccatcgt
     3181 aaatccccat ataatatgga ttttcctcat taaaagcacc ttttcctaat gaaagttgtg
     3241 ctactggtat ctgtgtttga tttacaaaat cttctaattc ttgatggagg tgaaaactgt
     3301 taatttcatg tccagtaatg atgataggct gcttcgcttg atgcaattta gatgctaata
     3361 actctatata tgttgacgca tcagtatctt tggctgccgt tacttcgaat ggtgtcggta
     3421 tttcaatttc agagattgcg acatcgattg gtaaatgtaa atgaactggg cgtctttcgg
     3481 cgattgctgt attaattaaa cgtggtattt cggttgttgc attttcaggt gtgatataac
     3541 cttgtgcaac ggttatatgt gcaaacattt ttcggtagtc gtcaaatgta ccttcaccaa
     3601 gtgagtgatg tacatattta ccggcttgtt caacagcacg tgtcggcgca cctgtaatcg
     3661 caatgacagg tatgcgttca gcatacgaac ctgcgatacc gttgacggca cttaattcgc
     3721 caacaccaaa tgtagtaact aatgcagcga gtccattaag acgggcataa ccgtccgctg
     3781 cgtaacttgc gtttaattca tttgtatttc ctacccaatc tacattggga ttactgataa
     3841 tatcgtctag aaaagcgaga ttaaaatcac caggaacacc aaaaatttta tcgacgcctg
     3901 ctcgatgaat agcgtcaatt aagtaagctc caatgcgttg tttcataaaa aaaccacctt
     3961 ttctctatat ttacacttaa aatgatagcc tcgaatatag tgtaaggtca aggaagttga
     4021 ctttgttgtg aaaacgctaa caatagtgag caattaatat tttttaggaa atgagtatga
     4081 agggggattt aattgatgat tttaaaattc aaaatttaag gggagagcga actatcaatg
     4141 atgctgcaaa acacaataac aatgaaaaaa caggcgcgtc accttatgaa ggtatacgca
     4201 cctgtttata gtaagcatta tttagcttca aataattgat cgccaaatga aatgttgcca
     4261 tgttcacctt gtttaaaatc aaggtttgta atgtttcctt gtgtaacgat aataggagta
     4321 atatcactct ttgcatgatt gcggatgtag tctaaatcaa agttgattaa taaatcacct
     4381 tgtttaactt cttgaccttc ctcaacatgt aaagtaaagc cttctccgtt taatttaaca
     4441 gtgtctaaac cgatgtggat taatagttct aaaccactat ctgatacaag accaattgca
     4501 tgttttgttg ggaaaatcat ttgtacttta ccgttgaatg gtgcacgaac ttcaccttgt
     4561 gaaggtttga tagcgatacc gtcacccatc attttttcgc tgaacacttg atcaggcact
     4621 tctgataatg gtgttacttc accagttaat ggtgcatgca cgatatggct caattcgctt
     4681 gttgcagatt tatcttctgc aacaacaaca gtttcgtctt tatcgtcttc catagtagta
     4741 gggttttcta ctacttgacc attcataatc tgttgcattt catgtttgat ttggtcagat
     4801 tttggaccaa aaattgcttg catattattg ccgacttcta atacaccaga tgcgcctaaa
     4861 tctttcaaac caggaacatc aactttagat ttgtcgttaa cttcaacacg tagacgtgtg
     4921 atacaagcgt ctaaatgttt aatgtttgct ttgccaccca tagcttctaa tactgcatat
     4981 ggtaattcag ttgctgaagc agtagccgct tgtgattgtt tatcttcacg acctggtgtt
     5041 ttgtatttta attttacaat taggaatcgg aatacgaagt agtaaatgac tgcgtataca
     5101 agacctacag gaatgactaa ccaccattgt gtcttattag gtagtatacc gagtaagaag
     5161 tagtcgatga aaccacctga gaatgtataa cctagatgaa gatctaataa gtacaatgtt
     5221 aagaatgata aacc
//
LOCUS       DAPIDM010000158         5069 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000158 DAPIDM010000000
VERSION     DAPIDM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.159"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..377
                     /locus_tag="RJE42_002388"
     CDS             <1..377
                     /locus_tag="RJE42_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV5877782.1"
                     /translation="GEDIVECITNFADDQDLTFTSVSGIGACDDVILKFFNLTTKQYE
                     EKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSGHLAKAIVSATAEIILT
                     VTDLDIQRSFNDAVGLNLLDPQ"
     gene            564..1181
                     /locus_tag="RJE42_002389"
     CDS             564..1181
                     /locus_tag="RJE42_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDV5877783.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLGEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVFVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            1178..1462
                     /locus_tag="RJE42_002390"
     CDS             1178..1462
                     /locus_tag="RJE42_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877784.1"
                     /translation="MTINIIVLIVSIIVFQLMIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(1617..2990)
                     /locus_tag="RJE42_002391"
     CDS             complement(1617..2990)
                     /locus_tag="RJE42_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV5877785.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DPKALYIKTYLPIFNQIDAKYLHDTHRNESELFKQGIELGRHYPKQIVDHQEKRSQVL
                     ATFKALD"
     gene            complement(3076..4629)
                     /locus_tag="RJE42_002392"
     CDS             complement(3076..4629)
                     /locus_tag="RJE42_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDV5877786.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFTILIVILLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         1701 a          893 c          732 g         1743 t
ORIGIN      
        1 aaggcgaaga tatcgttgaa tgtataacaa actttgcaga tgaccaagac ttaacattta
       61 catctgtcag cggcattggt gcatgtgatg acgtcatttt aaaatttttc aatttaacaa
      121 cgaaacaata cgaagaaaaa catattactg agccattgga attgacgagc ttattaggta
      181 atatttctcg tctagacaat ggtcattttg cacatttgca tgctactttc ggtactcaat
      241 catatgaaac ttttagcgga catctcgcta aagcaattgt ttctgcaact gctgaaatca
      301 ttcttactgt aactgatttg gacattcaac gttcattcaa cgatgcagtc ggtttaaatt
      361 tacttgatcc tcaatagcac tttacttttt agttgaatca aattttatta caagttatca
      421 taagtgctta atcataaatg cattctcgtt tattaatata cttcaacgag ttaaattaaa
      481 tttttaatta ggacttgaat gcgtttataa ctatcacatt tgaatgaata acttttttca
      541 ttagaaagta gtgtttatta ttgatgcaat ctcgatacat cgctagaatt tactttttaa
      601 tattatttat cgtctccctt tttgagacta aaatttttca atttatgact ttaaatttgt
      661 ttttagcata tatacctttt gagcttagtc tattattacg attattcaaa ccgaataaaa
      721 agtatgaatg gccactcttt atcatatata gttttatatt tgtgttactt ttaccaaata
      781 cattttatat ggtaactgat ttaatccatt tgaaccaatt tcaattcaac ttttacgcag
      841 gactaaattt aggtgagtgg aaatacttta cctatttact gcttggtgtc ttcttagcgg
      901 tttatgtgat gattttaatc tattttgaaa tactaacatt gacttctcac cgttggctta
      961 atagattaat tgttttcgta ctaatgtttt taaatggatt cggaatttat attggtcgtt
     1021 tcttacgctt acattccgtt tatttcttta atgaaccctt acatgttcta aatcacattc
     1081 tagaaagtct aacgcttaaa actgctatgt ttgtatgttt tatggttgtg atgcaagcag
     1141 caattctatt atttggaaaa ggagtgcgtt tgaacaagtg acaataaata taatcgtttt
     1201 aatagtatct atcattgtct ttcaattgat gattggacac atatggcatg atattggttt
     1261 aagttattta agaagtatct tactcatgat gctaccattt ggactaggtg tatttataca
     1321 acaagtgtcg tattacgaac gacaatatcc taaatggcaa gtgcctcaaa acataaaagt
     1381 tagattgaaa tatatttatc tcgctacatt tttagagtat gtcgtactat acctcacttt
     1441 atttacggac atactaagat aacaccaaat taataagaaa actaaataca acttatatat
     1501 ttgagcaaag ccaatttcta tcacatcaat aatagaaatt ggcttattta tattgcttaa
     1561 gaactttata ttctgctcca gattttactc atgcctgatc tatcaattga gccgaattag
     1621 tctagcgctt taaatgtcgc taaaacttga gatctctttt cttgatgatc cactatttgt
     1681 tttggataat gacgacctaa ctcaatccct tgtttaaata attcagattc atttcgatgt
     1741 gtatcatgca aatatttagc atcaatttga ttaaaaatcg gaagatatgt tttgatatac
     1801 aaagctttag gatcaaagcg ttcactctgt cttattggat taaacattct aaaatacggc
     1861 actgcatccg tacctgtaga agcagaccat tgccatccat gaatatttga tgctgcatca
     1921 tagtcaataa ggtgctttct aaagaatttt tctccccatg tccaatctat aaataaatct
     1981 ttggttaaaa attgcgacac aaccattctc attcgattat gcataaaacc agtttgtgtc
     2041 aattccatta ttgctgcatc aatgattgga aatcctgttt gcccttcgca ccatgcatta
     2101 aaatccgctt cattttgcga ccattttatc tgtcgatatt taggtttgaa agcttggtat
     2161 gaggtttctg gatactgtgt cattaacaca taataaaatt ctctaaaaat gagttcacgt
     2221 ataaatgctt catagtttgc ctcatcttca tcataacgcg ccaataaatc attaaaaatt
     2281 tcaataatat ctaataatcc atatgctaaa gcaacactta gctgacttgt taacacttca
     2341 ggtaaatagt ctcttccgct ttcgtaattc tgtatatctt gatctaaaaa atgttgccat
     2401 ttattctgtt caattatttg tgaggatcca aatgctatgt catcaaacac taaatcatca
     2461 ggtgatgcaa tgacgaagtc ttcgaatgat tttaaatcat agtgatatac gtctctatgc
     2521 ctcaagtaag gtcgccattt tttatagaaa ctagtaaaaa ctttgtatgg ctccccttgt
     2581 tgattcattg ttttactcgc tttaaagtaa tgattcccac gtatcaaagt aacggcaatg
     2641 cgtgcttcat tgaataaaga gcgttgatgt aaaatatcat attcttcaag gtgataactc
     2701 ataatatcac ctgcaatcac tacttcagag atattgtttt ctttacaaaa tgaaatgata
     2761 tcgtcataag acatgacata aggtgtgagc ttttctgcgt gcaacgattg gtaaaagcgc
     2821 tgcaaagtac cgcgataaaa ctgtgctttc aaatcagaag ctgaagacat gtcctcttcc
     2881 ggaatgataa aataacatgc atcaatgtct tctttttgtt gataaatata ttcaaataat
     2941 ggattgtgtt ccattcgaaa cattcgattt aataacacag ctattgccat aaacaaaaca
     3001 cccttcaatt atctttttat tttattaagc tttttgtcac tttaaaataa cctagattct
     3061 tatttatatt ttaaattaaa gcaaacctaa ccatttccaa taagtcatac taaaaactat
     3121 cactagaata taccctacaa ttgtcaatgg tatacctgcc ttcaagaaat ctttaaccgt
     3181 aaaagtacca gtgccataag ccaacatatt ttgaggtgca ctaacaggta ataagaaacc
     3241 aaaactaata acaaattgtt gaattaaaac aaatcctata gactggtctc ctaagtgtaa
     3301 cgtagaggtt agcgaaataa aaacaggtat taacgctgat gataaacttg ttgcactcgc
     3361 aaagcccaaa tgaatcaata tattaaaaag cgtgataagt gcaattgtcg cgataatagg
     3421 taaatgtttt aaacctaaaa caccaaaagt ttgatcactt aaccattgag ctgcacctgt
     3481 tttcaaaaga acgttaccta gtgaaatacc tacaccaaac acgataattg ttccccatgg
     3541 tattttattt tcaacatgtt tccatgtcat gacaccaatt ttcggcatta acataacacc
     3601 taaagcaata atagtaatgg atgcagagtc aatcggatgt aatacttttt cagttgacca
     3661 aaacagtaat aataacatcg atataacaat taaacgccat tcacgtgcgc taacggggcc
     3721 aagtttatgc aattcttctt ttattaaatc tttgccaccc tctattgtat taatttctgg
     3781 aggcatcact ttaatcatga tgaaatataa agctatagac ataactatgg accaaggcgc
     3841 tgcatataag aaccactcgc cccatgaaac atcaaatcct aattgatggt ttataaaatt
     3901 aatcgctacg atattttgtg ctgccgccgt tttgatacca atattccaaa ttgacacagc
     3961 ttgtactgaa gtaattatta ataaagacgc taacttgcta tctttagaaa ctttaaatgc
     4021 cgcaatcata cctagcaaga ttggtacaac tgcccctgct ctagctgttg cagaaggaac
     4081 gaaaaatgca agtacaattg aaacaatgat tgctccaata actatatttc tagttttatt
     4141 accaacaatt gataacacta aaagagctag tcttttatgc aaattcgttt cttgcatagc
     4201 agcagccaaa aataatgcgg ctgcaacgag agctacagct gaagttgcaa atccactaaa
     4261 cgctaatgat aatgcatgat tagttcctag aaggtcactt ccagctaaaa cagcactgcc
     4321 acttttcgga ttacctagct tctcccctaa attttgaacc gggctaaaac ccaaaagtaa
     4381 tatcattaag ccaataatta aagttgctga caccggatat gatacagctt ccgttaccca
     4441 cataataact gcaaaagcta aaatagctag tactgcctta gccattattg gcagacttga
     4501 aggtgctggg agtaatagta taactataag tattgtaaaa ctcataacaa tccaaaatgg
     4561 cttgtattgc ttttttgtct tttctttctt ttgttgttca tgttccacta catgtttttc
     4621 ttctgacata acagtcaccc ctttgtttta tcttaattta attataacaa ttttttaaaa
     4681 catacatttt taatttatgc aaaatacaca attttatcga actattttgt aaataatatt
     4741 gctaggtgat gagttatctc ggttaattac aaaacgatat ctaagtttaa atattagatc
     4801 ataaaaaaac tatctactga atatcctcat agcatcattt ttctatgcaa aagttaaact
     4861 tagttagata aaatgcaaat gagattattg tagatagtct ctttttaata tttaaaaatg
     4921 attaatgcca taaacataaa taattccaaa gaatgccgcg ccactaagca ctgttaatat
     4981 aagacttagt aacaatgtac gtttataatg tttaataaat gctatgaaca aaatgactac
     5041 attatacgca aagaaaagtc cgaataatg
//
LOCUS       DAPIDM010000159         5040 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000159 DAPIDM010000000
VERSION     DAPIDM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.160"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            55..2043
                     /gene="tkt"
                     /locus_tag="RJE42_002393"
     CDS             55..2043
                     /gene="tkt"
                     /locus_tag="RJE42_002393"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV5877787.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESIIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2321..2563
                     /locus_tag="RJE42_002394"
     CDS             2321..2563
                     /locus_tag="RJE42_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDV5877788.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2550..2648
                     /locus_tag="RJE42_002395"
     CDS             2550..2648
                     /locus_tag="RJE42_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877789.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            2742..3209
                     /locus_tag="RJE42_002396"
     CDS             2742..3209
                     /locus_tag="RJE42_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDV5877790.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLVIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3333..4454
                     /locus_tag="RJE42_002397"
     CDS             3333..4454
                     /locus_tag="RJE42_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDV5877791.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDTFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFENNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEGAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFSYNEENNAIEIGE
                     KDDMSIIEMFYNHITDKELSDIQSKKIKNILENELRKED"
     gene            4458..>5040
                     /locus_tag="RJE42_002398"
     CDS             4458..>5040
                     /locus_tag="RJE42_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV5877792.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNNRIYKVHRQGPFIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIEILGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFNSEKFEAIREILKEEVKKE"
BASE COUNT         1836 a          677 c          953 g         1574 t
ORIGIN      
        1 tttaaaataa gttataatgt atttgataaa attgaagaag ggaagataca caagatgttt
       61 aatgaaaaag atcaattagc tgttgatacg ctacgtgcac taagtattga cacaatcgaa
      121 aaagcgaatt ctggtcatcc aggattacct atgggagctg ccccaatggc ttacactttg
      181 tggacacgtc atctgaattt taatccacaa tctaaagatt acttcaatag agaccgtttc
      241 gtattatctg cagggcatgg ttcagcacta ttgtatagct tgttacatgt ttctggtagt
      301 ttagaattag aagaattaaa gcaatttaga caatggggtt ctaaaacacc aggtcatcct
      361 gaatacagac atacagatgg tgtagaagtt actactggac cacttggaca aggttttgct
      421 atgtcagttg gattagcttt agcagaagat cacctagcag ggaaatttaa taaagaagga
      481 tataatgttg tagatcatta cacatatgta ttagcttctg acggtgattt aatggaaggt
      541 atttcgcatg aagcagcttc atttgctgga cataataaat taagtaaatt agttgtttta
      601 tacgattcaa atgatatttc attagatggc gaattaaaca aagctttttc tgaaaacaca
      661 aaagctcgtt ttgaagcata tggttggaat tacttactag ttaaagatgg taatgattta
      721 gaagaaattg ataaagcgat tactacagct aaatctcaag aaggaccaac aattattgaa
      781 gttaaaacaa caatcggatt tggttcaccg aataaagcag gaactaatgg tgttcatggg
      841 gcacctttag gtgaagatga aagaaaatta acattcgaaa attacggttt agatcctgaa
      901 aaacgtttta atgtttcaga agaagtatac gaaattttcc aaaatactat gttaaaacgt
      961 gctaatgaag atgaatctca atggaattca ttattagaaa aatatgcgga aacatatcct
     1021 gaattagcag aagaatttaa attagcgatt agtggtaaat tgcctaaaaa ttataaggat
     1081 gaattaccac gttttgaact tggtcataat ggtgcatctc gtgctgattc tggtactgtt
     1141 attcaagcaa tcagtaaaac tgtcccttca ttctttggtg gatcagcaga ccttgctggt
     1201 tcaaacaaat ccaatgtaaa tgatgcaaca gattatagtt ctgaaacacc tgaaggtaaa
     1261 aatgtgtggt ttggtgtacg tgaatttgct atgggtgctg ctgtaaatgg tatggctgca
     1321 catggaggtt tacatccata tggtgcaaca ttcttcgtat ttagtgatta tttaaaacca
     1381 gcgttacgtt tatcatcaat tatgggatta aatgctactt tcatcttcac acatgattca
     1441 attgcagttg gtgaagatgg tcctactcat gaaccaattg aacaattagc tggactaaga
     1501 gccattccaa atatgaatgt tatccgtcct gcagatggta atgaaacaag agtagcatgg
     1561 gaagttgcct tagaatctga atctacacct acttcattag tattgacacg tcaaaactta
     1621 ccggttttag atgtaccaga agatgtagtt gaagaaggcg ttagaaaagg tgcctataca
     1681 gtttatggct ctgaagagac accagaattc ctactattag cttcaggttc agaagttagt
     1741 cttgcagttg aagctgctaa agatcttgaa aaacaaggta aatcagtgcg tgttgtttca
     1801 atgcctaact ggaatgcatt tgaacaacaa tctgaagaat ataaagaatc aattattcca
     1861 tcaagcgtaa caaaacgtgt tgcgattgaa atggcttcac cgcttggatg gcataaatat
     1921 gtaggtactg caggtaaagt tattgctatt gacggctttg gcgcaagtgc acctggcgat
     1981 ctagtagttg aaaaatatgg ctttacaaaa gaaaatatct taaaccaagt tatgagctta
     2041 taagaataat ttataaagcg agtatgttta gaagtctagg atgcataatc ttaggcttct
     2101 tttaaagtgt tgaaatttag agtatagcac tcaaactaca tcataagtga taagttttga
     2161 aaatatacta tttcagatta atctttaaaa tctctgttat aacagcatga tttttgatat
     2221 tatttttagt atcgatatta aaatacttga ataaactagt tcttgaaata atgtgatgaa
     2281 tttagtaaaa ttcagtagga taaagaaagc gggtgaaaca atggcaactt ggttagcaat
     2341 tatttttata gtagcagcat taattttagg tttaattgga ggtttccttt tagctagaaa
     2401 atatatgatg gactacttga agaaaaaccc accaatcaac gaagaaatgc ttcgtatgat
     2461 gatgatgcaa atgggtcaaa aaccttctca gaagaaaatt aatcaaatga tgacgatgat
     2521 gaataaaaat atggatcaaa atatgaagag tgcgaaaaag taaattcgca attgatagag
     2581 gctattttcc agatatggaa atggcctctt tttataatca aattaataag aataaatatg
     2641 tttattaaaa ttaaagttaa caaaatgacg aatagactga gaaatgctat aattcatttt
     2701 gtatgattta cagagagttt atttaacgag aaggtgtctg cgtgctctat ttaatatttt
     2761 caatcattgt agctttattt atgggaacta tagttatagt tattcgtatg aaagctcaaa
     2821 attatccggt aaatgagaaa aaaatagttt tgccaccgtt ttttatggcg actggtgcat
     2881 tgatgtacgt cgttccatat tttagactaa caggatcgga aatgctagaa gcttttataa
     2941 ttggtttgct tttttccaca gttctaattt ggacttctcg atttgaagtc aaaggtacag
     3001 aaatttatat gaaacgatct aaagcatttc cagttatttt gatttcatta cttgtcattc
     3061 gtactgtgat gaaaatattc attagtaatg aaatagatcc tggagaatta ggcggcatgt
     3121 tctttttatt agcattctgt atgattgttc cttggagagc agcaatgcta tataaataca
     3181 aaaaactaaa gaaaacatta atcaattaat tactttcaaa accacttgtg atcgacttct
     3241 aaatcagtca atgagtggtt ttaattttac ttgaaaaggt gaaatgatta tattaaaata
     3301 aaagcgaaca aatgttctat atggaggcag taatgaaaat tatacataca gcagactggc
     3361 acttagggaa aatattaaat ggcaaacagc ttttagaaga tcaagcgtat attttagata
     3421 cgttcgtaga aaaaatgaaa gaagaagaac ctgatatcat tgtgatagct ggagatttat
     3481 atgacacaac atatccaagt aaagatgcaa tcatgttatt agaacaagcg attggaaagc
     3541 tgaatttaga actgcgtata ccaataatta tgattagcgg aaatcacgat ggtaaagaga
     3601 gattaaacta tggggcgagt tggtttgaaa ataaccagtt atttataaga acagatttta
     3661 catcgattaa ttcaccaata gaaataaatg gggttaattt ttatacactc ccttatgcta
     3721 ctgtgagcga aatgaaacac tattttgaag atgacaccat tgaaacgcat caacaaggaa
     3781 ttacgcgctg tattgaaaca atagcaccgg aaattgatga aggtgccgtc aatattttaa
     3841 ttagtcatct gactgttcaa ggtggaaaga catctgattc tgaaagacca ttaactattg
     3901 gaacggttga atcagttcag aaaggtgttt ttgatatatt tgattatgtc atgctaggtc
     3961 acttgcatca tccatttagt atagaagacg acaaaattaa atatagtggc tccttattgc
     4021 agtattcatt ttcggaagcg ggtcaagcaa aagggtatag acgtgtaaca attaatgatg
     4081 gcattattaa cgatgtattt attcctctta agccgcttag acaattggaa attatctcgg
     4141 gcgaatataa tgatgttatt aatgaaaaag ttcatgtgaa aaataaagat aattatttac
     4201 attttaaact taaaaatatg tctcatatta ctgatccaat gatgagttta aaacaaattt
     4261 atcctaatac tttagcgctg acgaatgaaa cttttagtta caatgaagaa aataatgcta
     4321 tagaaatagg tgaaaaagat gacatgtcaa tcatcgaaat gttttataac catataactg
     4381 ataaagaatt atcggatatc caatctaaaa agataaaaaa tattttagaa aacgaattga
     4441 gaaaggagga ttaatgaatg aaaccattac atttaaagtt gaataatttc ggcccctttt
     4501 taaaagaaga aattgatttt tctaaaattg ataataatga attgttttta ataagtggta
     4561 agactggatc gggtaaaaca atgatttttg atgcaatgac ttatgccttg tttggtaaag
     4621 catcaactga acaaagagaa gaaaatgatt tgagaagtca tttcgctgat ggtaaacagc
     4681 cgatgtcagt aacgtttgaa tttcaattaa ataatcgaat ttataaagtg catagacaag
     4741 gcccttttat caaagaaggt aatacaacaa aaacaaacgc taaatttgat gtatttgaga
     4801 tggtggatgg caagtatgaa attagagaaa gtaaagtaat ttcaggtacc caattcatta
     4861 ttgaaatatt aggagtaaat gcagatcaat tccgacaatt gtttattttg cctcaaggtg
     4921 aattcaaacg ctttttaata tcaaacagtc gtgaaaagca agggatatta agaacactgt
     4981 ttaacagtga aaaatttgaa gctatacgag aaatattaaa agaagaagta aaaaaagaaa
//
LOCUS       DAPIDM010000160         5039 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000160 DAPIDM010000000
VERSION     DAPIDM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.161"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(151..651)
                     /locus_tag="RJE42_002399"
     CDS             complement(151..651)
                     /locus_tag="RJE42_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDV5877793.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            792..1910
                     /gene="ald"
                     /locus_tag="RJE42_002400"
     CDS             792..1910
                     /gene="ald"
                     /locus_tag="RJE42_002400"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDV5877794.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDNPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            2034..2147
                     /locus_tag="RJE42_002401"
                     /pseudo
     CDS             2034..2147
                     /locus_tag="RJE42_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2226..3281)
                     /locus_tag="RJE42_002402"
     CDS             complement(2226..3281)
                     /locus_tag="RJE42_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDV5877795.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEINKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSQEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            3456..4145
                     /locus_tag="RJE42_002403"
     CDS             3456..4145
                     /locus_tag="RJE42_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV5877796.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(4651..>5039)
                     /locus_tag="RJE42_002404"
     CDS             complement(4651..>5039)
                     /locus_tag="RJE42_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDV5877797.1"
                     /translation="DTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQASIKAGVHL
                     NKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEIVVLSNRKAE
                     DKKKFVLGSVSHKVAKRATIPVLIVK"
BASE COUNT         1569 a          811 c          897 g         1762 t
ORIGIN      
        1 ttttcacgaa tatcttaaaa aaattaagta gtcgaagaag attatgacaa cattattaag
       61 tgagttttat ttataaacaa taaaaaacca agtgcagaaa gttgcacttg gttcataatg
      121 atcaagtagt tatctaatga tcatctatat ttaattttga tatttttcac gtaattgagt
      181 tgttgcaact tgtggttgga agtctgctgg taactcttca gtacgaacaa ctaacacgtc
      241 gcatggtgca tgacgaacga tagattctga tacagaacca acaataaatc tttccacggc
      301 atttaagcct gatgtaccac tcataatcaa gtctgcatta atttcatgtg caagcttttt
      361 aggaatgata gatttaggag agccaaactc tagacgcgtt tctacatctt taacaccagc
      421 gttagtagct acttctttat aaccatttaa taattcttct gcaaaatgct tagatttttc
      481 agtaaattga gcatcataaa cttcataaga agaatatgtt cttgaatcaa ttacattcac
      541 aattgttaac ttcgcatcgt tacgttttgc aacaccaact gctctgttaa atgcccattc
      601 cgcttcatgt gaaccgtcaa ctgcgattaa aatatttttg taagtaatca tagtaagtgc
      661 ctccttgttc tttcttaatt atattttacc acaattttcg gaaaagtcag tgtaataacc
      721 tttacaattt tgtggatttt tgattgcgct ttcaaaatgt tcgcgttaag atgattatgg
      781 aggtggatgg tatgaaaatt ggtataccaa gggagattaa aaataatgaa aatcgtgttg
      841 gtttatcacc aagtggtgta cacgctttag tagaaagtgg gcatactgtt ttagtggaaa
      901 caaatgcggg ttcaggatcg ttctttgaag atgtagatta caaagaagca ggtgctgaga
      961 ttgttgctga acaagcaaaa gtttgggatg tggatatggt tattaaagtt aaagaaccac
     1021 ttgaatctga atatccatat tttaaagaag gactagtatt atttacttat cttcatttag
     1081 caaatgaaga aaaattaaca caagctttga tagatagaaa agtaattagt attgcatatg
     1141 agactgtgca gttaccagac cgatctttac cattgttatc accaatgagt gaggtagcag
     1201 gaagaatgtc agctcaagtt ggcgcagagt tcctacaaaa actcaatggt ggtatgggaa
     1261 ttctacttgg tggcgttcca ggagtaccta aaggtaaagt aactattatc ggtggtggtc
     1321 aagcaggaac aaatgcagct aaaattgcac taggactagg tgcagatgtt acgattttag
     1381 atgttaatcc aaagcgttta caacaattag atgatttatt cggtggacgt gtacatacaa
     1441 ttatgtcaaa tccgttgaat attgagttgt atgttaaaca aagtgattta gtaataggtg
     1501 cagttttaat tccaggtgct aaagcgccaa gacttgtaac agaagacatg attaaacaaa
     1561 tgaaaaatgg gtcagttatt attgacattg ctattgatca aggcggtatt tttgaaacaa
     1621 ctgataaaat tacgacacat gataatccta catatatcaa acatggtgtg gttcattatg
     1681 cagttgcaaa tatgccgggt gcagtaccgc gtacttcgac gttagcttta aataatgcta
     1741 cgctacctta tgcgctcatg ctagctaata aagggtatag agaagcattt aaatcaaatc
     1801 aaccattatc attaggttta aatacttaca aaggtcatgt aaccaataaa ggcgttgcag
     1861 aggcatttga aatggaatat aaatctgtag aagaagcatt acaattataa tttgaattta
     1921 atgaaaaggg aatggattac agcgtactaa agaatgacgt tatcatctcc ttggttatta
     1981 aaattaaata atgataaatt aaagctagaa acaatgcgaa gaaatcatcg aacatgaatg
     2041 atattgatct gattcattaa atgttactga tgaacatacc tgcatatgtg tttctagctt
     2101 gtttgttaca tattgcattt agacttggac aaaaatttat gtcctaggcg ctacaatgtt
     2161 atattggcag tgtttgactg aatgagaata cgcttgtaac aagcttttca tttttgtccc
     2221 attccttatt tttcataatg tgttaatact tcgtatcctt cattagtgac aagtatgtca
     2281 tcttcaattc ttacacctgc aacacctggt acataaatac ctggttcgat tgtaataacc
     2341 atgccagctt ctaacaaatt agaattagta cttgaaacat cttgatattc atgttcctgt
     2401 aatcctagac catgacctaa gcgatgaggg aaatattcac cataaccttt ttctgaaata
     2461 atgtttctag cgatatgatc gatatctttt aaaggtattc caggtttaat tgcttggatt
     2521 gcagatgttt ctgcttctaa tacaatatta taaatttctt gtgcttcttg gctaggttca
     2581 ccaaatttaa tggtacgtgt catatcgcta caataatgct cataaattac acctaaatca
     2641 aatagtacat actcattgct ttttaatctg cgatctcctg gtgtgccatg aggtgatgcg
     2701 gcatgatctc caaataaaac catcgtatca aaactcattt cattgacgcc atattgtttg
     2761 attgtttgct caatatggtt gactacttca cgttcagtca caccttcttt taaataagaa
     2821 acacctattt cgatacactt atctgctaac tcagcagctt tacgtatttt gttaatttca
     2881 tcttcagatt taatatttct caattgtttg attgttaaat caacatctcc gaatgaattg
     2941 acattgaaac cagagattaa ttgtttttgg cgtgctactg ttaagtgctc gctttcaatt
     3001 agtaatttat tgattgtttg aggataaagt gaaaaaggat tttcagtgtc taaatatcca
     3061 acgatttcac ctgtgaaagg tgatgctttg acttcttcga cttccatttt tggacaaaat
     3121 agtacttgtt taccatcttt cttaatcaat aatgcaaata atctttcatg gggttcgcta
     3181 cggtatccag taaaataata tacattcaac ggtgttgtaa tccatgctgc atcagcttgt
     3241 tgattgttca gttcgtctat tatttttgat atttttgtca ttttataaac actcctcaac
     3301 ataatattac ttacatttta tgctaattat tattatattt caaaatcaag ctaatacatt
     3361 aattagattt tcaattttta aaatttgaga atttgttata attaagaggc aaaagggtac
     3421 taaatttata tagcaattga taggaggatg ctcaaatgaa gttatcattt cacggtcaat
     3481 caacgattta tttagaaggg aacaacaaaa aggttatcgt agatccgttt atttcaaata
     3541 accctaagtg tgatttgaat attgaaacag tgcaggtgga ctatatcgtt ttaacgcacg
     3601 gtcattttga tcattttggt gatgttgtgg aactagcaaa gaaaacagga gcaacagtaa
     3661 ttggaagtgc agaaatggct gactatcttt cttcatatca cggtgttgaa aatgttcatg
     3721 gtatgaatat tggtggtaaa gcaaactttg attttggaag cgttaaattt gttcaagcat
     3781 ttcatagttc aagttttaca catgaaaatg gtattccagt ttatttaggt atgccaatgg
     3841 gtatcgtgtt tgaagttgaa ggaaaaacaa tttatcatac tggtgataca ggtctgttta
     3901 gtgacatgtc acttattgcg aaacgtcatc cagttgatgt atgttttgta ccaattggag
     3961 ataattttac gatgggtatt gatgatgcaa gttatgctat taatgaattt attaaaccca
     4021 aaatttctgt accgattcat tatgatacat tcccattaat cgaacaagat ccacaacaat
     4081 ttaaagatgc tgttaatgta ggggacgtgc aaattttaaa accaggtgaa tctgttcagt
     4141 tttaaataga atatagattt tacactaaaa acacctcgct aatctatgtg gaagttatcg
     4201 tctgcatatt tttagtgggt gtttttatat tctactgata ttgtaagctg actttttcgt
     4261 cagcttctgt gttggggccc accccaactt gcacattata gtaagctgac tttttcgtca
     4321 gcttccatgt tgggacccca gcccaacttg cattgtctgt agaatttctt ttcgaaattc
     4381 tccatgttgg gaccccaccc caacttgcac attaatgtaa gctgactttt ccgtcagctt
     4441 ctatgttggg ggccccatag tcaactcctg ctgggttatg atattgatta tgtttttggt
     4501 tcaaaatggt tttgtttgat acaagttgtt ggttaaatag tgaacaatat aaaaaaacca
     4561 ggaccacagt cataatcatt tcttgtgttt actcagtcgt ttgagactgc atcatgattg
     4621 aaagattatt tgtgattctg gataaaaatt ttatttaacg attaatacag ggatagtcgc
     4681 acgttttgct actttgtggc tgacacttcc aagtacaaat ttctttttgt cttctgcttt
     4741 acggttactt aaaacaacaa tttcgtattt accgctatta gcatgtttta atagttcttc
     4801 ttttgcatta ccacgaacaa ttatttggtc ataatcaata ccataatctt ctaaagcttt
     4861 gcgtgttttt tctaatcgct tgcttcgttc ttcagtaagt ttgtttaaat gaacacctgc
     4921 tttaattgat gcttgagcat cttgttcgct gattgcgttt aaaactgtta cgactgtacc
     4981 ttcgccagct aatttagaca cttcttttag tgctttttca ttttttaact gagtgtcta
//
LOCUS       DAPIDM010000161         4970 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000161 DAPIDM010000000
VERSION     DAPIDM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.162"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(11..1087)
                     /locus_tag="RJE42_002405"
     CDS             complement(11..1087)
                     /locus_tag="RJE42_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDV5877798.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPAFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            1174..1485
                     /locus_tag="RJE42_002406"
     CDS             1174..1485
                     /locus_tag="RJE42_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDV5877799.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            complement(1610..2452)
                     /locus_tag="RJE42_002407"
     CDS             complement(1610..2452)
                     /locus_tag="RJE42_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV5877800.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHITADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGESIDAYILRETL
                     VDNN"
     gene            complement(2976..3620)
                     /gene="trmB"
                     /locus_tag="RJE42_002408"
     CDS             complement(2976..3620)
                     /gene="trmB"
                     /locus_tag="RJE42_002408"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV5877801.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKNGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(3635..4426)
                     /locus_tag="RJE42_002409"
     CDS             complement(3635..4426)
                     /locus_tag="RJE42_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDV5877802.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT         1591 a          930 c          658 g         1791 t
ORIGIN      
        1 tattacccga ttatttatat tgtaatgttt ctatttgatt gttatctaaa ttacaaatgg
       61 cttctgaaag taaagatcta gctgcaaaat agtctcttat atcaaatact gagtctgtac
      121 tatgaatata tcgtgcacat acaccaataa ctgcagtcgg aataccaata ttagctttat
      181 gaatttctcc accatctgtt ccacctggtg acatatagta ttgatgttca atgtcatgtg
      241 cttctactaa ctttaataaa tagtccctaa atgcaggctt taaaatcatt gtaccgtctt
      301 ttatgcgaat taacgtacct ttaccaagtt caccggataa tggttggtta cctttaacgt
      361 cattggcagg tgaacaatcg acaacaaatg caacgtctgg gtctatcatc tctgcagatg
      421 ctttcgcacc tcgtaatcca acctcttctt gaacatttgc gccaacatac aagtctacat
      481 ctaattctat atcttttaat aattctagta tttcaattgc caagacacaa ccataacgat
      541 tatcccatgc tttagcacta tatcgatgtt cagataactg tgtgaatggc gtgtgaggta
      601 caattgtatc tcctatatct attccgcgct cacgtacttc atcttcattt tgagcaccta
      661 tatctaatgt taaatcttta atttccggtg caccttcact accagtacga aaatgtttag
      721 gtatattaga aacaacaccg ataattttat cgccatttct atttttaatt actaagcgtt
      781 gtccttgcca aatatcattt gcaacaccac ctaaatttgt gaattgaatc attccatttt
      841 tagtgatatt tgtaatcatg aatccaattt catccatatg tgctgcaatc attacacgtt
      901 ttgcatttgg atttttagat tttttaacac caaaaaaacc acccatacga ttttcaataa
      961 attcatctac gtacggcgcc atttgctgag tcatataatt ttttacttct tcttcaaaac
     1021 ctggtgcccc atgaagctca gttaaagttt gaattcgttg taatgttact tttttattta
     1081 tgttcataaa agttctcact ccttataagt tattatatca tcttcaatat ggtaaactaa
     1141 tgatatgaat ttgaaatcaa gggagcataa atgatgacta aactgaaata tataattcca
     1201 acaataattg cagtagctat tgtaattatt tctaccattt caatcatcca atttattaat
     1261 cgtaaacgct ataatcccgt taaagtactt aatgaagtaa aatcatattt tatgaatgtc
     1321 aaaggctcgt atatcgttta cgaaccattc gttcatcctg aaactgataa ataccgttta
     1381 gtttatcaag gtggaattac aactattaaa aatggtcaaa atattcatta tgatttttat
     1441 gcagatgcat atactggtga agtcattaac attgtagagc gttaatacga attttatcca
     1501 tatttgattt ggatcgtaaa acttaaaaat gagccacacc ctaattagta agataggatg
     1561 tggctcattt ttttattctt gaaaatttgt ttagtaatac atctttattt tagttattat
     1621 caactaatgt ttcacgtaaa atatatgcat ctatactttc gccatcatcg ctgtatttta
     1681 cagcaaagta gttttcatca tgataaaaca agaaccaata ttgttgctgg ataaaatatg
     1741 gtatcatgcg ttccttttca cgaattgatt gcataggata atcatcatat gctgttaccc
     1801 atagaggatt tttatgtgca gtagtcggga atatatcacc catatgaact gctttatctc
     1861 cttgactttc aatcgtaatg attgtgtggc caaagctatg acctccacta tgttgcatct
     1921 tgatacccgg aaccggttca aaatgttttt cgaataaaat caacttgtta ctataatcgc
     1981 ctttattctt atcccagtaa gttgatttac ttcttatatt aggtgcaata aactcatgcc
     2041 actcatcttg ttgcacaaca tgaatcgcat tttcaaaaat tgcatgtccc gcttgatcag
     2101 tcaaaccggc agcatgatca aaatgcatat gtgtcattag cacataatca atatcctttg
     2161 gcgttaaatt ataatttgcc aaatcagcag ttatatgact ttcttcatct acaccaaaat
     2221 tacgtaattg cttttcagat aatttaccat taccaatacc cgcatctata atcaaattat
     2281 attgagccgt ttgaatcaaa attggatgtg tcggtaaatt gatttgattt cgttcatttg
     2341 cattgtattg ctttgaccac aaaggcttcg gaacaacacc aaacattgca ccgccatcca
     2401 tttttgtatt gccgccattt agataatgaa tagatatatc cccgattttc ataacatcac
     2461 ctattctttc tgtattcgtt attacaatta gcatgtgttt attacatcat atcactattc
     2521 atgaatgcga acaaaaacga aacgccccac actaatacac ttccaactac ttataaaatt
     2581 taatgcaatc acattatatg taaatgcatc ataaccatcg gactgtaatt ttaaattaat
     2641 aattaaaatt tccaagtaaa atgcaagatt tattctttta aatcaaaggt gtttagtata
     2701 acattattca taggcgcaat tgtctccttt agtttaggtt tggtcacttt aaattataga
     2761 ggcaatagcg cttttttgta ttaaaaaaca gaatgttcca cctttccacc ataaaaggtt
     2821 aagaaccttt ttatattcaa aatcaaaaaa tcggacatat gaaaattttt tcatcgtccg
     2881 atttattata gaaacaatag ccttttctac agtatcattt attatagtac gaaaaaacac
     2941 ttatgattgt atgtgtacat accctcataa gtgctttatt tttgtgaatg gaatttcgct
     3001 tccatacgat aaatacgtga ccctttatcc gaaaatttct tttcatattc tgttaaaata
     3061 ttactgccat cgtcttcttg atgtaaattt agatttattt ttgtaaaata cattccaaat
     3121 tgagacatac tttctaaact gtaggcaaat agtcctctgt tatcagtttt aaagtgtaaa
     3181 tctccttcat catttaagat ttgttgatac aacgctaaaa acgtatgata cgttaaacgt
     3241 cgttttgcat gacgattttt tggccatgga tctgaaaagt tcaaataaat acgagaaact
     3301 tcgccgtttt taaaatattc atttaattca atggcatcat tacaaataat ctttaaattt
     3361 gttaaaccca tctctttaac tttatctaat actttataaa cgatactttt ctcacgttcc
     3421 attgaaatat agttaatatg aggattttga gcagctaatg ttgtaataaa ctgccccata
     3481 cccgaaccaa tttcaatgtg tatcggttgc gttttatcaa accattcagt cattttccct
     3541 gcatgttgac cgtccatgtc aaccaattca ggatgatctt ttaaataatc ctcagcccat
     3601 ggtttgtatc gaactctcat attttattct cctcttaaat aaacatgtta ctattcataa
     3661 cttcatttag gaatttaagc caagtgttca tatccttata tcttttttgc tcttcatacc
     3721 attgaacaag acctatagat tgaattaccg tataccattt catacgttta tttaaattca
     3781 agctctcttg aactccatat gtttcaagcc attcagacca ttgttgttgt ggaacatagt
     3841 tgtaaagcag cattccgata tcaattgccg ggtctgcaat cattgcacct tcccaatcga
     3901 ctaaaaatag ttcatctcga tcggataata accaattatt atgattcaca tcaccatgta
     3961 caacagtgaa aaaacgcgaa tctaaactcg gtatatgctc ttctaaatag gttaatgatt
     4021 ttctcacaat atgatgtgtt aaaacttctc ttgataaaga ggcattaatt ttattaagca
     4081 taatctcagg agtaataggt tccatttcca tacgctttaa catactcaat aaaggtctag
     4141 aattgtgtat cttctttaat aaatgtgcaa ctcttgtttg cttcatttca tttgaagata
     4201 gttcacggcc atttttccaa tgttgtgctg taacaacctc gcctgtttct atgcgtttcg
     4261 tccatactaa tttgggcaca ataccttctg ctgataatgc cgcaataaat ggatttgaat
     4321 ttcgttttaa aaacaacttt tgtccatctt gttcagccat atatgcttca ccagatgcac
     4381 cacctgcaga atcaagtgtc caccctaatt gataaaactg ctccaactcg tccacctcac
     4441 tttcaattag aaaatggctc tagaaatagg tttttcaaga gccatatatt ctaatttata
     4501 acaccatgct ggtacaaatt ttatgtccag ataattattt taaatcctca accaatgcct
     4561 acattacacg actaaattta aatcgtaatg tctgtcattg acaccataca ttctatagtc
     4621 acttacttga cataaaatgt taccgtgtct aaaactacat gtttttgtaa cttttgtagg
     4681 cgataaactc tagttttcaa aataattgct atcccatttt catggttagc ataaatttat
     4741 gaactgtaac atttacgtac ttagtaaaat atgatgcaca tcatatttgt aactcataga
     4801 aaattttata atttttatca ttatatttca actgaaaatg agaaacaaaa tggcactttt
     4861 tactaatatg tgttttctaa acaacacttt taagcttcgt tttaaattat aacataattc
     4921 acttacgaaa gttgataaat ttaagtaatt taatctaaaa atatgatgaa
//
LOCUS       DAPIDM010000162         4807 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000162 DAPIDM010000000
VERSION     DAPIDM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.164"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(30..299)
                     /gene="rpsN"
                     /locus_tag="RJE42_002410"
     CDS             complement(30..299)
                     /gene="rpsN"
                     /locus_tag="RJE42_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV5877803.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            complement(753..902)
                     /gene="rpmG"
                     /locus_tag="RJE42_002411"
     CDS             complement(753..902)
                     /gene="rpmG"
                     /locus_tag="RJE42_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV5877804.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(993..2510)
                     /locus_tag="RJE42_002412"
     CDS             complement(993..2510)
                     /locus_tag="RJE42_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDV5877805.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADTEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2714..4168
                     /locus_tag="RJE42_002413"
     CDS             2714..4168
                     /locus_tag="RJE42_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV5877806.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMASIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPKFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVIATLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYGNQK"
     gene            4308..4412
                     /locus_tag="RJE42_002414"
     CDS             4308..4412
                     /locus_tag="RJE42_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877807.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            4387..4701
                     /locus_tag="RJE42_002415"
     CDS             4387..4701
                     /locus_tag="RJE42_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877808.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
BASE COUNT         1501 a          845 c          710 g         1751 t
ORIGIN      
        1 gtaaatgggc taaagtacga aatttgattt taccaacttg attttttaac acctggaatt
       61 tgtcctttgt gcgcatgttc tctaaacgca atacgagaca tttcaaattt acgtaataca
      121 cctctaggtc ttccagttac tttacatctt ctagttaaac gtgtaggtga tgaatctctt
      181 ggtaattttc ttaacgcttc gtaatcacct tttgctttta actctttacg taattcgtaa
      241 tatttattta ctaactcttc tctttttctc tcttttgcta ttttagattt cttagccata
      301 tatagttacc tcacttttta tcaaatcgta attattacga tttatataat aacacaatcc
      361 tcacataatt caacagtaaa gtttcatttc tttgttaaat cattttttaa accatcaaat
      421 ttaacatcaa agtgtattgt cgaacattta taaaacatat ccacaattac ttcttactta
      481 ttaaattccg ctttgaaatt ttaaattcta ctatattaaa tactctttca agcattcgct
      541 aacagagtca ctttataaaa tcagtaatct atttccttct atcaatagtc tcaattttta
      601 ataaagtaac aactaataca aacatatatt gttctatcaa tgaaactcaa ttcattaatc
      661 actcttcaca aaaatttcat aaaaaagcga gctcccccat ataataggta agcccgcttt
      721 attttcaaat cgtcgtattt gaatgataag aattacttag tttcacgatg taacgtatat
      781 ttgtttaatc ttgggcaata ttttttcatt tcaatacgct caggattatt acgtttattt
      841 ttagtagtga tatagttacg atcgccacat tctgtgcatg ctaatgttac gtttacgcgc
      901 acgttaaaca ccctctcttg ttaggaatct ttacgattta ttatgatatc ataaactgct
      961 caactacgca atatagaaac tacatatcaa atttattttt caaagttttc atatgtttca
     1021 tcattttcag tttcaagatc aatagaattt atatcaatac ctaatgcttt tgcaacacct
     1081 ttaccatatt ctgtgtcagc tttgtaacaa tgacgaatat gacgtcgttt aacatcatcc
     1141 gttacgcctt ccattgcatt tgctgtattt gtaaaaattc tttctttagc gtcctctgat
     1201 tgtaatctaa acaatttacc tggttgttca aaataattat catcatcttg acgttgatta
     1261 tattcatagc cgtatccatc agttgggaat ggtggctttt tatattcagg ttgagaatca
     1321 aatttaccat ggttatttgg ataataatgt gttcctccac cttggttatt gtcaactaca
     1381 cgcatttgac catctctact aaaaggacaa atattttcaa taccaacacc tttaggttgg
     1441 tttacaggaa tctgccaatg attaactcct aatcgatatc tttgcgcatc gccatatgag
     1501 aataaacgcc cttgcagcat tttgtctgga gaaaaatcta atcctggaat aatattagtt
     1561 ggtgcaaacg cagcttgttc aacatccata aagtaattat ctggatttct atttaattca
     1621 aactctccaa cctcaattag aggatactca tcgtgatacc atacttttgt taaatcaaat
     1681 ggattatctt tatggttttt agcttgttcc tcagtcatta cttgaatata catcgtccat
     1741 tttggataat cacctttttc aatggcttcg aataaatcgc gttgagatga atcgcgatct
     1801 gttgctataa tttcagcagc ttcttcatca gttaagtttt caataccttg ttgcgttcta
     1861 aaatggaatt taacccaaac acgttcacca gaatcattat acatagagta tgtgtgtgaa
     1921 ccgaacccat gcatgtgacg taaatcttta ggaatccctc tatctgacat taagatcgtt
     1981 acttggtgca atgcttctgg aagccccgtc cagaaatccc agttattttg tgcatctctc
     2041 atatttgttc taggatctcg tttcaccgcg cgatttaaac taacaaatag ctttggatct
     2101 ctaaagaaga atactggtgt gttattccct actaaatccc agtttccttc ttctgtgtag
     2161 aactttaacg caaatcctcg aatgtcacgc tccgcatcag cagcaccacg ctctcctgct
     2221 acagtagaga aacgggcaaa catttcggtt tgcttaccta tttcagagaa tattttagca
     2281 ttcgtatatt ttgttatatc tttagttact gtaaatgtcc caaatgcacc agaacctttg
     2341 gcatgcattc gacgttctgg tattacttct ctatcaaatt gagacatttg ctctaaaaag
     2401 taaatatctt gcattaaaag aggtccccta ggccctgctg tcatactatt ttctcgatct
     2461 gatactggat gcccaaaaac accagttaac tttttgtctt gttgtgacat agtcatccct
     2521 ccacaattta taataattat aatttaatac atattataat ttaaccccat cagaattaca
     2581 attaaacatg ctcaagatga ttaaaaaatc agaaaatttt aattttttcg ctttacagta
     2641 ctaaatagtt attgaatagt acacttaaaa agcttacaat aatgggtata tttaaataga
     2701 tgggagacta aatatggaag ataataaaat gaaccgtagt cttaactcaa gacacatttc
     2761 catgattgct ataggtggtg caattgggac tggtctattt gtagctactg gtaatatcat
     2821 ttctcaagct ggtcctggag gcgctatact cgcttatctt gttattggtg tcatgctata
     2881 tttcttaatg gcatcaattg gagagttggc aacattttat ccagtatcag gttcattcag
     2941 ctcttattca acacgcttta ttgactcatc tctaggcttt accatgggat ggttgtattg
     3001 ggcattgtgg tcattagtta caagtgttga tgtcatagta gcgtcaaatg tgctttattt
     3061 ctgggacaca tttaaatttt tccaccccat tacttggagc ttaatcttta ttacaatttt
     3121 actattatta aacatttttt ctgtaaaatc atttggagaa actgagtttt ggttatcatt
     3181 aattaaagtg ttaacaatca tcgtattcgt tatttttggc tttttaatga ttttcggtat
     3241 cttaggtggt catgcatatg gatttgaaaa ctatacaaaa ggccaagcac cgtttgttgg
     3301 tggtatctct ggtttcttag gcgtattatt agtcgctgga ttttcggttg gtggtacaga
     3361 agtagtagca gtaactgctg gtgaatcaga tgaccctaaa aagtctatgc ctaaggcaat
     3421 taaacaagta ttttggcgta ttcttttatt ctatgtctta tcaattgcag taattggtgc
     3481 aattattccg tacacagatc catcattatt aagagcaagt agttcaataa gtcaaagccc
     3541 atttacaatt gtattcgata gagtaggcat agcctttgca gcatcagtaa tcaatgcggt
     3601 tattttaact tcgctattat ccgctgcaaa ttcaggtgtt tatacaacag gcagaatgtt
     3661 gtattcctta agttcagaca aaaaagcacc taaattttta agtaaattaa acaagacaac
     3721 aaaattacca ttaagagcat tattaactac atatgcagtc gttgttattg ttattattta
     3781 tgcaaacttt aattcaaatg ccgtttttaa tttacttgaa attattggtt caatgattat
     3841 agttgtttgg ggatcaagca tttggtcaca aatacgattg cgacaagcta ttaaaaaaca
     3901 aggtcaagat cctaataagg tcctaccata taaagcacct ttttatccat taggaccaat
     3961 cattgtcatc gctacgctat tattcttgct atttggtggt tcagttgaat atattttaaa
     4021 agatcaatgg ttaaatgctt ttaaaaactt tttaccttta atcattctag cgttgattta
     4081 ctttattcat aaaatcattc acaaaacaaa atttgtaaag ctagaaacaa ttaatttaaa
     4141 accacacgat tatggcaatc aaaaataaat aaattcccac acggcagatg ctttagatta
     4201 tctatagcat ttgccgcttt tttaatggtg taaagtagat atactttaat atctttttct
     4261 agtataaagt gtattaacta gtattctgtt tatattttat tatgaccatg ttaccattct
     4321 ttattttaga tattttgcat atcgtgtata atatagataa caataacttt tcaggggtga
     4381 aattcaatga ttggacaaac aaatttattt gatgatgtac taaagcaaga tgagagattt
     4441 gttattgtag ttcaagcatt agaagaaaag aacggacaat tattaaagag aactttaagg
     4501 gaatatcccg gtttaaacca taaacaaatg aatgatttat ttacgcactt aaaggaatta
     4561 ttttccgaag aatcatttgc tgaaaaccaa tcagcgttca gtattacagt ttatacaaat
     4621 ttagattata ctgctgacca aatatatgct catgtaaaac gtttcagagg gaagcatgac
     4681 tggacacaaa cagctaaata aaatcaagat gcttgcagtg attttaacaa ttaatgttag
     4741 attgtcactg cattttttaa tacattcgtt attattcaga atgattcaaa tttatttatc
     4801 tacctca
//
LOCUS       DAPIDM010000163         4780 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000163 DAPIDM010000000
VERSION     DAPIDM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.165"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            1445..2416
                     /locus_tag="RJE42_002416"
     CDS             1445..2416
                     /locus_tag="RJE42_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDV5877809.1"
                     /translation="MSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTD
                     NNDIDEIIAWRGEQAELEQKRDTYKKRYHEFEMEIARLESLTKNKELLDSDKLKDEYE
                     LKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKN
                     NKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHS
                     NKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETA
                     LDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(2487..2849)
                     /gene="mscL"
                     /locus_tag="RJE42_002417"
     CDS             complement(2487..2849)
                     /gene="mscL"
                     /locus_tag="RJE42_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV5877810.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFVFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            3053..4699
                     /locus_tag="RJE42_002418"
     CDS             3053..4699
                     /locus_tag="RJE42_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDV5877811.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         1781 a          640 c          823 g         1536 t
ORIGIN      
        1 tgacctttta tggcaagaaa ttgaatcatt tgatgatgac aaaataaaag gcttattaga
       61 ggttgccact caacagatag acaaagtgat tgaaaatata ccacttttac aagctaggtc
      121 gaaagaaata ctagcatttg taaatgaaag taaagaaact gctattaaag aatatgaaat
      181 aatagaaaag aaaacactag aaaataatat attaaaagat aatattaatc aactcaacat
      241 aaataaaatt gatttcgttc aattgaaaga gcaacaacct gaaatagagg aaattgaagc
      301 taagttaaag ttgttacaag atattacaaa cctattgaat tatattgaaa atagagaaaa
      361 aattgaaact aaaattgcta atagcaaaaa agatatttct gaaaccaata ataaaatatt
      421 gaatcttgtt tgtgataagc gaaacataga caaagagaaa aaaatgttag aagaaaatgg
      481 agatttaatt gaaagcaaaa tctcttttat tgataaaact agagtattat ttaacgatat
      541 taataagtat caacaaagtt atctcaatat tgaacgcttg agaacggagg gtgaacaatt
      601 aggtgatgaa ttaaataatc taattaaggg tttagaaaag gtcgaagatt caataggtaa
      661 taatgaaagt gattacgaga aaattatcga actaaataat gcgataacta acataaataa
      721 tgaaattaat gtaattaaag aaaatgaaaa agctaaagct gaattagata aactattagg
      781 tagtaagcaa gagttagaga atcaaattaa tgaagaaaaa actatattga agaatctcga
      841 aataaaatta gatcgatatg ataaatcaaa attggactta aatgataaag aaagctttat
      901 aagtgaaatt aaatctgctg taaagattgg agatcaatgt ccgatatgtg gtaatgaaat
      961 tcaggattta gggcatcata ttgattttga cagtattgct aaacgtcaaa atgaaattaa
     1021 agaaattgaa gcaaatatcc acactatgaa atcgaatatt gctgtacata attctgaaat
     1081 taaatttgtt aatgaaaaaa tatcgaatat taatattaaa acgcaaagtg atctctcact
     1141 cgaagtgttg aataagcgtc tgctagaaaa tgaaaatgca ttgaataatc aaagagagct
     1201 taataaattt atagaacaaa tgaaagaaga aaaagataat ttaacgttgc aaattcataa
     1261 taaacaattg cgtctaaata aaaatgaatc tgagttgaaa ttatgtcgag atctcatcac
     1321 tgaatttgaa actctctcaa aatataataa tatcactaat tttgaggtgg attataagaa
     1381 gtatattcaa gatgtgaatc aacatcaaga acactcaaag gagattgaag ataagttaat
     1441 acaattgtct caaagaaagt taattgagca aaataatcta aatcactatg aaaatcaact
     1501 agaaacttac aataatgact tagaattgaa tgaacagtct attgaaatgg aaatgtctag
     1561 gctgaactta actgataaca atgatataga tgaaataata gcctggagag gcgagcaagc
     1621 ggaattagaa cagaaaaggg atacttataa aaaacgttat catgaatttg aaatggaaat
     1681 agctaggtta gaatcattaa ccaagaataa agagttattg gactctgata aattaaaaga
     1741 tgaatatgaa ttgaaaaaag gaaagatgaa tacactgata gatgaatact ctgctgttca
     1801 ttatcaatgt caaaataata ttaataaaac acaatctata gtttcgcata ttaattactt
     1861 aaatcaagaa ttaaaggatc aacaagaaat atttcaattg gctgagattg tcagtggtaa
     1921 gaacaacaaa aatcttacat tggaaaactt tgtcttaatt tactatttag atcaaattat
     1981 tgctcaagca aatctgagat tagcaacaat gtcagataat cgatatcaac taattaggag
     2041 agaagcggtt tctcatggtc ttagtggcct agaaattgat gtatttgatt tgcattcaaa
     2101 taagtctaga catattagct ctttatcagg tggagaaact ttccaatcgt cgcttgcatt
     2161 agctttaggg ttaagcgaaa ttgtacagca gcaatcagga ggtatttcac tagaatcaat
     2221 atttattgat gaaggattcg gtacattaga tcaagaaacg cttgaaacag cgttagacac
     2281 tttattaaat cttaaatcaa ctggtagaat ggttgggatt atttcacatg tgagcgaatt
     2341 gaaaaataga atacctttag ttttagaagt gaaatcagat caatatcaga gttcaacaag
     2401 attcaaaaga aattaaaaca taaaaaatag agatggtact ttggaatgta tagaacaacc
     2461 ttagcccatc tctttttatt taaaaattat tttttctcac gtaataaatc tctgatttca
     2521 gttaataaca caacattttc ttccacaact gcttcttctt cggcttcttc tttcttcatt
     2581 aatgtatttg caatcttaac aaagacgaat aaagcaaacg cgatgataat gaagtcgata
     2641 acagattgga taaataaacc gtatttaata ccccagaatg accattcttt agcaaaatca
     2701 actgatccga aaattttacc aattaatggc ataatgatat tttctactaa tgaagatata
     2761 atcttgttga aagctgcacc catcacaaca gcaattgcta aatctaagac gttacctttt
     2821 aaggcgaact ctttgaattc ttttaacatt acactcaacc tctctttttt aaatgtaacc
     2881 tcaattatac aacaaataaa aaaattagaa aatagattat ttaagaaaat ttgattatcg
     2941 caaagacctt tgttatccta taaaggtgcg tgagttattg aataaaatgt ttagataata
     3001 tttttattct actacataaa attaaatata gaattgaagg gagtaattaa ttatgaattc
     3061 ttcttcacca gagaatccaa atggaaagaa gtattcacca gtcttcatct atagtgcaat
     3121 tgttgttgct atagtcgtat tacttggtgc atttttacct gaacaattca actatgttac
     3181 caataatatt aaaatgtgga ttacagaaaa gttaggttgg tattatctta ttcttactac
     3241 gattatcgtg ttcttctgta tattccttat ttttagtcct attggaaaac ttaaactagg
     3301 taaaccaaat gacaaacctg agtttaatac tatttcatgg tttgctatgt tgtttagtgc
     3361 tggtatgggg ataggtttgg tgttttatgg tgcagctgaa ccgatggcgc actttgctac
     3421 gccacctacg gcagatccca aaactactga agcttatact gaagccctac gttcaacatt
     3481 tttccattgg ggattccatg cttgggctgt ttatggtgtt gttgcgttag cgttggcata
     3541 ttcgcaattc cgtaaaggtg aaccaggttt attatctaga actttacgtc ctcttttagg
     3601 tgataaagta gaaggtccta ttgggatttt tattgacgtt ttatctgtat ttgcgacaat
     3661 cgttggggta gctgtttcgt taggtatggg tgctctacaa attaatggtg gtttacatta
     3721 cttgttcaat gttccaaaca atacgtttgt acaagcgatt atcatcattg ttgttactat
     3781 cttatttata gcaagtgcat ggtctggatt aagtaaaggt attcaatact taagtaactt
     3841 gaacattggt ttaggtacta ttttaatggt agctgcttta attgttggac caactgttct
     3901 tattttaaat atgttaacta gctctacggg tagtttacta aacacattct tgtttaatag
     3961 ttttgataca gctgctttaa atcctcaaaa gcgtgaatgg atgtcttcat ggacacttta
     4021 ttattggggt tggtggttaa gttggagccc attcgttgga gtgtttattg cacgagtttc
     4081 aaaaggacga tcaattagag agttcatttc tggtgtcttg ctagttccag caattgttag
     4141 ttttgtatgg tttagtgtct ttggtgtatt aggcatcgag acaggtaaga aacacaaaga
     4201 aatttttgat atgactcctg aaacacagct atttggagtg tttaatcatg tgccatttgg
     4261 cattgtttta tcgttgattg cattattatt aattgcatca ttctttatta catctgctga
     4321 ctcagcaaca tttgtattag gaatgcaaac aacatttggt tcattaaatc catctagtat
     4381 ggtaaaagtt gtttggggaa tttcacaggc cttaatagca tttgtacttt tattagctgg
     4441 tggcggtaac ggcgctgaag ctttaaatgc gattcaaagt gctgcaatta taagtgcatt
     4501 cccattctcc tttgtcgtca tactcatgat ggtaagtttc tacaaggatg cgaaccagga
     4561 acgtaaattc ctaggtttaa cattgactcc gaataaacac cgcttacaag aatatatcaa
     4621 gagtcaacaa gaagattatg aatctgacat tcttgaaaag cgtcagtcac gtagaaatat
     4681 agagaaaaaa gataactaat aaaatttagt caagtatttt aataacaagt agtttgtcat
     4741 tctagtagct agaacagata tactacttgt ttatttttgt
//
LOCUS       DAPIDM010000164         4760 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000164 DAPIDM010000000
VERSION     DAPIDM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.166"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            132..803
                     /locus_tag="RJE42_002419"
                     /pseudo
     CDS             132..803
                     /locus_tag="RJE42_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            1205..1885
                     /locus_tag="RJE42_002420"
     CDS             1205..1885
                     /locus_tag="RJE42_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HDV5877812.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLK
                     GYYSGTGFNFQNVSGYRENDKMNLFSGSQLNVISLLGSDKERFKNSDYSGLDVFVVRE
                     GSGRQADNNSIGGITKTNKNNYKDFVNNVGLEITKPTGHNTATRQVETYSINKEEISL
                     KELDFKLRKHLIDKHDLYKTEPKDSKIRVTMKNGDFYTFELNKKLQTHRMGDVIDGRN
                     IEKIEVNL"
     gene            1907..3394
                     /locus_tag="RJE42_002421"
     CDS             1907..3394
                     /locus_tag="RJE42_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDV5877813.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASEANEKPSTNQE
                     SKVVSQTEQNSKETKTVESNKNFVKLDTIKPGAQKVTGTTLPNHYVLLTVDGKSADSV
                     ENGGLGFVEANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGEDEEDEEVEEASTDKAG
                     AEEESTEAKATYTTPRYENAYKVPEKQLDKKNGHHQVLIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDDLELKK
                     DNEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVNKVKVLKDVKEDLH
                     VDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGESQLFPDLQVDEKG
                     EFSFDVDHAGFRLQNGETLNFTVVDPITGKLLSEKFVSKNIDIYESPEEKADREFDER
                     MENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(3504..3812)
                     /gene="spn"
                     /locus_tag="RJE42_002422"
     CDS             complement(3504..3812)
                     /gene="spn"
                     /locus_tag="RJE42_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDV5877814.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYSNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            4179..>4760
                     /locus_tag="RJE42_002423"
     CDS             4179..>4760
                     /locus_tag="RJE42_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5877815.1"
                     /translation="MGYLKRFTLYISILILIVVIAGCGKGNEIKEDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPGEIDDEGMVLYLNR
                     NTKKATGYYFVNKIYDDISKNQNEKKYRVELKNNKIILLDNVEDEKLKQKIENFKFFS
                     QYADFKDLKNYQDGSITTNENVPSYEAEYKLNNS"
BASE COUNT         1908 a          554 c          848 g         1450 t
ORIGIN      
        1 agttttattt taaatcttca aaatggtata gatttatggc attaaacggt gattcaggtg
       61 ctcgagcaga taggttttct attaaagata ggacatttat ggaaatgcca cttcatatcc
      121 catgtatgga tgaacaaata aaaatcggtc agttcttcag caaacttgac cgacaaattg
      181 aattagaaga acaaaaactt gaattacttc aacaacagaa aaaaggctat atgcagaaaa
      241 tcttctcgca agaattgcga tttaaagatg agaatggtaa agattatccg gagtgggaag
      301 aaactactat aaaagaaatt gctcaaatta acactggaaa gaaagataca aaagatgcca
      361 ttactaatgg gagttatgat ttttacgtta gatctccgat agtttataaa attaatactt
      421 ttagttatga aggagaggct attttaactg taggagatgg agttggcgta ggtaaagttt
      481 tccactatgt aaatgggaaa tttgattatc atcaaagagt atacaaaata tctgacttta
      541 agaattatta tggattattg ttattttatt atttttcaca aaacttttta aaagaaacaa
      601 agaaatatag tgcgaagaca tcagttgatt cagttagaaa agacatgatt gctaatatga
      661 aagtaccgcg tcctatttat atagaacaaa aaaaaatcgg tcaattcatt aaaagagtag
      721 acaacaaaac aaaaattcag aaacaagtga ttgaattact taaacaacgc aaaaagtcat
      781 tacttcaaaa gatgtttatt taagtctact ggaccttgtt ttaaaagtaa taatggtgtt
      841 tttcttatac tcttaacaat tggtttcaat taccaaaatt ataaaatcaa agtaaatcaa
      901 atacgaacat taaatttagg cactgtgata gcacagtgtc ttttttgtgt cgaaattgtg
      961 tacagaataa gtggtcaaat aaatattaag ttgagataaa gtgttattcg taaataaaag
     1021 agagtggatc gataggaatt gaatgatatt agttaactat ttattaaatt acttaataat
     1081 gattaatttt tagttaaagt aagtttaatg tgaagtacga ccgttgctca ttataatgaa
     1141 tgaggattgt tcgtattgcg taatagaata aatcaaatag actaaaaatt gggagcatag
     1201 aattatgaaa ttaaaaaata ttgctaaagc aagtttagca ctagggattt taacaacagg
     1261 gatgattaca actactgctc agccagtaaa agcaagtaca ttagaggtta gatcacaagc
     1321 tactcaagac ttaaagggtt attatagtgg aactggattt aattttcaaa atgtgagtgg
     1381 ttatagagaa aacgacaaaa tgaatctatt ttcagggtca caacttaatg taatatcact
     1441 acttggttca gataaagaaa gatttaaaaa tagtgattat agtggattag atgtatttgt
     1501 cgtacgtgaa ggatctggta gacaagcgga taataattct attggtggaa ttactaagac
     1561 gaataaaaac aattataaag atttcgttaa taatgtaggt ctagaaataa ctaaaccaac
     1621 aggacataat acagcaacaa gacaagtgga aacttatagt attaataaag aagaaatttc
     1681 attaaaagaa cttgatttta aattaagaaa gcatttaatt gataaacatg atctttataa
     1741 gacagagcct aaagacagta aaattagagt tactatgaaa aatggtgatt tttatacttt
     1801 tgaattgaat aaaaagttac aaactcatcg tatgggtgat gtgattgatg gtagaaatat
     1861 agagaaaatt gaagtgaatt tataataata ttcgagggag tatattatga gggaaaattt
     1921 taagttacgt aaaatgaaag tcgggttagt atctgttgca attacaatgt tatatattat
     1981 gacaaacgga caagcagaag catcagaggc taatgagaag ccaagtacaa atcaagaatc
     2041 aaaagttgtt tcacagactg aacaaaattc aaaagaaaca aaaacagtag aatctaataa
     2101 gaactttgtt aaattagata ctattaaacc tggagctcaa aaggtaacgg gaactacttt
     2161 accaaatcac tatgttttat taacagttga tgggaaaagt gcggattcag tagaaaatgg
     2221 cggtttgggt tttgttgaag caaatgacaa aggagaattt gagtatcctt taaataatcg
     2281 taaaattgtt cataatcaag aaattgaggt ttcgtcgtca agccctgatt taggtgaaga
     2341 tgaagaagat gaagaggtgg aagaagcttc aactgataaa gctggtgctg aggaagaaag
     2401 tacagaagct aaagctacat atactactcc tcgatatgaa aatgcctaca aggtgcctga
     2461 aaagcaatta gataagaaaa atggacatca tcaagttttg atcgaaccaa ttactgaagg
     2521 atcaggcatt attaaaggtc atacgtctgt aaaaggtaaa gttgctttat ctattaataa
     2581 taaattcatt aattttgaaa caaatgctaa tggtggtcca aataaagaag aagcgaaatc
     2641 tggatcagaa ggtatctgga tgcctatcga tgacaaaggt tactttaact ttgacttcaa
     2701 aacaaaacgt ttcgatgatt tagagttaaa gaaagataat gaaatctcat taacatttgc
     2761 acctgatgat gaagatgaag cattgaagtc attaattttc aaaactaaag taactagctt
     2821 agaagatatt gataaagcag aaactaaata tgaccatact aaagtcaaca aagtaaaagt
     2881 cttgaaagat gttaaagaag atttgcatgt agatgaaatt tatggaagct tatatcatac
     2941 agaaaaaggt aaaggtattc ttgataaaga aggcactaaa gtaattaaag gaaagactaa
     3001 attcgcaaat gcagttgtga aggtagactc tgaactaggt gaatcacaat tattccctga
     3061 tttacaagta gatgaaaaag gtgaatttag ctttgatgta gatcatgctg gatttagatt
     3121 acaaaatgga gaaacactaa acttcacagt agttgatcct attacaggta aattgttaag
     3181 tgaaaaattt gtttctaaaa acatagatat ttatgaatct cctgaagaaa aagcagatcg
     3241 tgaatttgat gaaagaatgg aaaacacacc tgcatatcat aaattacatg gcgataaaat
     3301 tgtcggctat gatactaatg gattcccgat tacttggttt tatccattag gtgaaaagaa
     3361 agttgaacgt aaggcaccaa aattagaaaa ataattaatt aaataaaaca gcttaatgat
     3421 gtaatgaaat tagtgagtta atcactgact tcaatgtcat tgagctgttt ttttatgtgc
     3481 tttgttataa agcattattg aatttatttt acgtgttcat attttgaaac atcaaagccg
     3541 tcttgcttag ctttgttgat aatgtctttg attgaatgta gtcctttatc ggcgaagtat
     3601 gatcttaagt tgtcttttgt agcttggtca gcattcttat ctaataacac atcgatataa
     3661 cttaattcat gttctaagaa gtttgcatca tcatgtagta cgagtccatt ttgagaataa
     3721 actttcgcat ctgcttgatt actatatcca acaacgccag ttgctaatac acctaccatt
     3781 gccgtagcta ctaaaacctt tttaaatttc atatctatca ctcctctaaa aattgtaact
     3841 ccatcataac actgaatatt gagaaaatta cgtttattaa gtctatttaa tgatttttaa
     3901 taaatagtta aagtgacaaa tattgtttaa atgcaattaa tctttagtgc gatgattatg
     3961 gattttccct actaaaacct tgtttacaaa gagggtttaa atcaaatgaa acaataataa
     4021 aaaataatgc aacataataa taagtacaaa tttaattaag aaattaaatt gattgtatat
     4081 gtatattttg gtaacgtaaa agagaaatat acaaaataat taattattta tatgaaaaga
     4141 gaatataaat gaagtataaa acagagagac gtgaagcgat gggatattta aaaaggttta
     4201 cattgtacat aagtatcttg attttaatag ttgttatagc aggttgtggc aaaggtaatg
     4261 aaataaaaga agattcaaaa gaaacacaaa tcaaaaagag ctttgcgaaa acattagata
     4321 tgtatccaat taagaatctc gaggacctat atgataaaga aggatatcga gatggcgaat
     4381 ttaaaaaggg agataagggg acgtggactt tactcacaag tttttcaaaa agtaacaaac
     4441 cgggtgaaat agatgacgaa ggcatggttt tatatctaaa tagaaatacc aaaaaggcaa
     4501 caggttatta ttttgtaaat aaaatttatg atgatattag caaaaatcag aatgagaaaa
     4561 aataccgtgt tgaacttaaa aataataaga ttattctttt ggataatgta gaagacgaaa
     4621 aacttaaaca aaaaattgaa aattttaaat tttttagtca gtatgccgat tttaaagatt
     4681 taaaaaatta tcaagatgga agtataacaa ctaatgaaaa tgtaccgagt tatgaagcag
     4741 aatacaaatt gaataatagt
//
LOCUS       DAPIDM010000165         4753 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000165 DAPIDM010000000
VERSION     DAPIDM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.167"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            149..787
                     /locus_tag="RJE42_002424"
     CDS             149..787
                     /locus_tag="RJE42_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877816.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVNFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(1034..2965)
                     /locus_tag="RJE42_002425"
     CDS             complement(1034..2965)
                     /locus_tag="RJE42_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDV5877817.1"
                     /translation="MTTTFIISYIVLALIIVGVINLFLIRSRRKAKRQQKEQQFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNKDNQNQVSLNKQTEGSEQDQELFNDNK
                     DDEAFAAKNPNSEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLKFP
                     TFTLTTVINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSVIALILCLFLQSN
                     KKAKATAQKIDADNSINHE"
     gene            complement(2978..3625)
                     /locus_tag="RJE42_002426"
     CDS             complement(2978..3625)
                     /locus_tag="RJE42_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV5877818.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            3746..4300
                     /locus_tag="RJE42_002427"
     CDS             3746..4300
                     /locus_tag="RJE42_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV5877819.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKRNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQNQ"
     gene            complement(4424..4621)
                     /locus_tag="RJE42_002428"
                     /pseudo
     CDS             complement(4424..4621)
                     /locus_tag="RJE42_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1611 a          823 c          680 g         1639 t
ORIGIN      
        1 ttgccaaaaa aagtatataa tgtagaagat attttctttt tcactttcaa atttaagact
       61 acaattgaac agtgattttt catcattata acagacaact agacatattg ataagtaaag
      121 aaaagaactt tatacggagg tgccttgtat gacaaatcca aatcaacgat tagaaccatt
      181 tgatgagaca tttcaacaac cgaatattca tcgtggtaag cgatatggta agaaaaaacg
      241 ttcattggta agcatgatta ttcaaatcat tgttgttata ttaaccgcaa tcgctggata
      301 cagcatgtgg aaacaaccca tttttaatat tgtcttcacc aaagaatctg taaattttga
      361 tggcattaaa aattttaacg agacaattgg aaaaatgcat caattaaata ttaatttagg
      421 taatatcaat gatttacaac aaggaatcga tcgcttaatt ttagtcttta acggtttctt
      481 tattttatgt atcgttagcc tagtcataac aatactgaca attgtattta atcgcacagt
      541 tctaaaagta gtaaatattt tagtgttaac aattatgtta gtcattacat tgtatttcag
      601 ttatattatt aaagcactcg cacaagaaat tgcagatgct ttaaaaaaat attatttaaa
      661 tgtaccacca gatcaagtga ttacagaagc tgacgctatt cataacggca tcattttact
      721 gggctgtagc attggtttac ttgttataag cttattcttc cgtaatcgca tgcctcgatt
      781 aaaataatgt tatatagtag ggattgggac agaaatgtta ttttcacaaa attcatttcg
      841 ttgacccact tcctgctttt tctttattaa tgacctacta catcaatgcc aaattttaat
      901 ataatttctc taacattcta caaatcaaca aataaaaaaa gtatcgataa ccatatattg
      961 gcaccgatac tttaaaaatc atgtccccac aacataattt ttgattagtc acacttcaat
     1021 taatttattt aatttattca tgattgatac tattatctgc atctatcttt tgagccgtag
     1081 cttttgcttt tttgttgctt tgtaaaaata agcataaaat caaggcgatg acgctaaata
     1141 tcgttgcaac tataaatgca tcattaatcc cttcaactgt tgctagttta tttacaaatt
     1201 gtaataacac tttcattgcg ccttcttgac caccatattg cgatgctaat tcacgcatat
     1261 gatcttgtac aacaggattc gttttatcta actcttcccc aaaagctgat aaatgttgtg
     1321 tagtttgcgt tgtcattaca gtaactaaga ttgctgttcc tatagaacca gctagttgac
     1381 gcatcgtatt taagaaggca ttaccatggg atgccagtcg tcccggcaac gcattaatag
     1441 ctgctgttac cattggcatc ataataaatg ccataccaaa tgaacgtagt acatagatgc
     1501 ccatgattgt catatatggc gtatccatat tcaatttagt taattcccat gttgcataag
     1561 tcatgacagc aataccaaaa atggctaaag gttttaaacc aatcgtgtct aacaacttac
     1621 cagcaaatgg tcctagtaga cccataatta gagaaccagg taataataac aatccagaat
     1681 ctaatgctga gaaaccacgt aaattttgta agtaaattgg taataaaatc ataccaccat
     1741 acaagctcaa cattacaacc atattaatta ctgttgttag tgtaaatgtt ggaaatttca
     1801 atacttctaa attcaacatt ggcgccttca ttcttaattc tcttattaca aatagaataa
     1861 taaagataat accaatcgca aacattgttt ctatctcaac agaaccccag cctttgttac
     1921 ccgcttctga aaagccgtat aacaaagcac caaaaccaat cgtactaaaa atgatgcctg
     1981 ggatatcagc tttagggttt gttgtatatt gatataactt aaaccataca aaaccaacta
     2041 agattgcaat aattccgata atgaacatac cgtaaaacat cacattccaa tggtaatttt
     2101 ggacaatata acctgataat gttggaccga ttgcaggtgc taaaatcatt gcgataccca
     2161 ttgtacccat ggcagcacca cgtttttcag gtggataaat tgtaataata acaattgaac
     2221 ctaatggcat tagtacacct gcaccaatgg cttgtaatac acgtccaacc atcatgattg
     2281 ggaaattcat tgaaatcgca catattaatg aaccaattgt aaagagtact aacgcaacta
     2341 aaaataattt tcgatatgaa tatttattaa atagatacgc cgtaattggt attaaaatac
     2401 cgtttactaa catgaatccc gtcatcaacc attgccctgt tgacgcagaa atattaaatt
     2461 ccgtattaat ttttggcaaa gcaacattta ataatgtttg gtttaaaatc gcaataaaca
     2521 taccgaataa taatgccgct aatattttac cgcgtgaaac accttcacca aaaataaagt
     2581 ttttatgttc ttttttaatt ttctcattta ctttatattc ttctgagtta ggatttttag
     2641 cagcaaatgc ttcatcatcc ttattatcat tgaataattc ttgatcttgt tcagacccct
     2701 ctgtctgttt gtttagacta acttgattct gattgtcttt attgtcaata cgtgcctctt
     2761 cacgcgccgt atgttgtgac gattgatcag ttgttctgtc taaatcactt gctttaaatt
     2821 tagattgatt tgattgacgt gtcgcaaact gttgttcctt ttgttggcgt ttagcttttc
     2881 ttctagatct tattaaaaat aaattgatca caccaacaat aatgagcgct aaaacaatgt
     2941 agcttataat gaaggtcgta gtcatttaat gaccccctta atttttatgg atttttactt
     3001 cagcgttcat tccaggaaca acttgtttag acggttctga atctaacgta attttaacag
     3061 gtataacttg agatacttta gtatagttac cgtcactatt tgatgatggc attaatgaaa
     3121 agcttgcagc agtagctttt ccaatactat caactttacc tttaatagaa gctttttgac
     3181 cgtcaatagt cacatcaaca tctttaccaa cttcaacatc tttaatatct ttttcgtcaa
     3241 tatttgcagt tacatataaa tcatctaaat tgtaagcata agcaattgga ttgcctgctt
     3301 gtaccataga accttccatg ccatctaatt tagcaattgt acctttttgt ggcattttaa
     3361 gttccatatc tttagtttcg ccatcttgac cttgaacagt aactgttgct actttatcac
     3421 ctttatcaag tttatcacct tgcttaacat ttagtgattt gatttgccca gaagctggac
     3481 ttgctatttt aatttgatcg ccatttactt tagcattatc agttgttata taacttgtcg
     3541 ttttattcca gaaataaaaa ccagcaatcc caattgctaa cagtacaaca accgtaatga
     3601 cattaattaa tatcatcttt ttcatgaaac atttcctcct atttgctttt aaaattcact
     3661 ttatttatta tagagacatg ataactaaaa aaacaccaac aatttttata agagtgttgg
     3721 ttaaaattca caaaggtgta acaatatgaa acgacaagcc aaaatagaaa ttcaaaacgc
     3781 acttgttgat ttgatggctg aatatccttt tcaagaaata tccactaaaa tgatttgcgc
     3841 gtattgtaat attaatcgtt ctacttttta tgattattac aaagataaat ttgatttatt
     3901 ggataccatc aactcaaagc ataaagaaaa attccaattt cttctaagcg cgctacatca
     3961 taatttcgaa aatattaaac aggataagct taaactttat aaattcttca ttattattgc
     4021 taagtatatc aagcgcaatg agcagttttt caaagacatt ttagttacct atccaatgaa
     4081 aacattattc atagactata taaatttagc gagagattac tatcaacaaa ttatgaatga
     4141 ctatagtact acagtaacca ataaacagct ttatgttaca tatatcatag gcggtcaagc
     4201 tggggtattt attaattggc tttcgaatgg ttgtaatgaa tcacctgaag aagttgctga
     4261 cattttattg gctaatacga ttaaactaca aaaccaataa tacggtttca ttctaataaa
     4321 gaaatgctat aaaacaacta gataaagtta aacccaatgg atagatatta gtacatatgt
     4381 aacggtgcta tcaatataaa aatgcgcttg ggacattaag ctcatatgca tataagatga
     4441 tttttaacat catttttgga tgataggatg ttgcgccacg atgatgtcag aattcatcta
     4501 attcgctatc aggtatcgtt tcaacaattt cattaacata tcgcgaaata tcattttgag
     4561 gaattctaac agaagtttct attggtagtg taagttgggt catattatga tttttataca
     4621 taaggcacct cgttaattta gtttagtggt atttattaaa ttatacgaag ggacccaaca
     4681 cagaaaattc attttattaa attttacatt tatgtgcaag ttgggcaaag tgtcttattt
     4741 ttttaaagta ttt
//
LOCUS       DAPIDM010000166         4683 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000166 DAPIDM010000000
VERSION     DAPIDM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.168"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..389)
                     /locus_tag="RJE42_002429"
     CDS             complement(<1..389)
                     /locus_tag="RJE42_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDV5877820.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVVVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLV"
     gene            complement(682..1971)
                     /locus_tag="RJE42_002430"
     CDS             complement(682..1971)
                     /locus_tag="RJE42_002430"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV5877821.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHSNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            2052..2507
                     /gene="bcp"
                     /locus_tag="RJE42_002431"
     CDS             2052..2507
                     /gene="bcp"
                     /locus_tag="RJE42_002431"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV5877822.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2513..3463
                     /locus_tag="RJE42_002432"
     CDS             2513..3463
                     /locus_tag="RJE42_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV5877823.1"
                     /translation="MKVVGLNRMREVETELQQRFSDVDFKFYKKASEIPESDLANLDI
                     LVGYDGGINEAFLRRCTNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIVINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKETLLIEVLKSRVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            3560..4006
                     /gene="perR"
                     /locus_tag="RJE42_002433"
     CDS             3560..4006
                     /gene="perR"
                     /locus_tag="RJE42_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDV5877824.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT         1756 a          685 c          748 g         1494 t
ORIGIN      
        1 acaagtacaa acctgtaaaa ggcagtaata tagaaagaac cggtatgttg tcccataatg
       61 attaatgctg ttacactcgc caaagtagca acatgtgcta aattaaattt aaataaaata
      121 gcaataagta ctacgacggt aacacccata atgataaaat tatcactaaa aattgttacc
      181 attgtaacag aaagtatggc acctataatg ttgcctaatg cttgatcaga aactgtttta
      241 aatgaacgat aaatactagg ttgcattgca acaacagcac tgacaccagc taaggctttc
      301 agaccaacat catccggtag taaagaagcg atagacatag ctaaaataat ggctatacca
      361 gttttaaaaa tccgagctcc tagtctcaaa aataatgacg ccccttttaa gtttattgaa
      421 tatctaatat tcgtattcat tactgttata cacttactag ttacaaaatt caagcttatt
      481 tatagttgtt aaaataaatc atacataata ctgatagcga tgtaaaactt tagtcagaga
      541 ttaaaagagt ataaatttgt aaaataaaaa ctcacatagg gacatatcaa tttaaacgtt
      601 aattgttaac gatataaaat gaatctacta tgtgagcatt tgttttattt taattcaatt
      661 taaaatatac ttccttaaaa gttatttcat ttgactaaaa gcatagtctg cagcttttaa
      721 agtttgttta atatcttctt ctgtatgttc agttgttaag aaccaagctt caaatttaga
      781 aggtgctaaa ttaatacctt gatttaacat caatttgaaa aatttaccga acgcttcgcc
      841 gtcagaatgt tcaacttgat cataatgagt gactttttca tctgtaaagt acaatgttaa
      901 agatccgtaa atacgattaa ttgtagctgt gatattatgt ttttcgatta atttaagtaa
      961 accttcttct agttgttgac ctaagctgtc taatttttca taaacaccgt cttgttctag
     1021 tacttcgagt aatgcaatac ctgctttcat agataacggg ttaccagcca ttgtaccagc
     1081 ttgatatgca ggtcctagag gtgctacttg ttccataata tcttgacgtc caccatagcc
     1141 tccaattggt aaaccaccgc caacaatttt accaaatgca gttaaatcag ggataacacc
     1201 taataaatct tgagcggcac cgtaatggaa acggaatgca gtaattactt catcataaat
     1261 cactagtgtc ccgttgctat gtgaaatttc attaacctct tccaaaaatc caggttgagg
     1321 cattaccatt ccaaagttac caacaattgg ttctactaat actgcggcaa tttcatcgcc
     1381 ccaaaattca attgcttctt tatatgcgtt aatatcgttg aaaggaacag taatgacttc
     1441 acgtgctacg ctttctggaa caccagctga atctggagaa cccagctgag atgggccact
     1501 acctgctgca accaatacta aatcagaatg gccatgataa gatccagcaa attttataat
     1561 tttatttctt ttagtatatg cacgtgcaac acgaattgtt gtcatgactg cttctgttcc
     1621 agaatttaca aagcgaattt tctcaagaga tggaattgca tcccgtaatt ttttgctgaa
     1681 ttcaatttct agttcagtcg gtgtaccaaa taatacacct ttagcagctt gttcttgaat
     1741 tgctttagta atatgaggat gtgcatgccc cgtaataatt ggaccgtatg cttgaaggta
     1801 atcaataaat ttattgccat cgacatcata taaatatgca ccgtgtcctt ctttcataac
     1861 aacaggtgca ccgccgccta cagctttata agaacgagaa ggggaattga caccgcctag
     1921 aatatattcg tttgaaagtt gttgtaaacg ttcactttca ctaaaattca tttatatcaa
     1981 cctcttttaa tttaatattt tcatctaata tcgtatcata aaattattat aatgaagaaa
     2041 aaggtgatta tatgttgcaa aaaggagaac aatttccaat atttaaatta gaaaatcaag
     2101 acggaactgt cattacaaat gatacattaa aaggtaaaaa ggcgattatt tatttttatc
     2161 ctagagataa tacacctact tgtaccacag aagcttgtga ctttagagac aatttagaaa
     2221 tgttcaatga tttagatgtt gcagtatatg gtataagcgg tgattcaaag aaaaaacacc
     2281 aaaattttat tgaaaaacac ggattgaatt tcgatttatt agtagatgaa gattttaaat
     2341 tagctaaaga aactggcgta tatcagttaa aaaaatcatt tggcaaagaa agtatgggta
     2401 ttgtaagaac gacttttata atagatgaac aaggtaaagt attagatgtt atcgagaagg
     2461 ttaaagttaa aacacaaata gaagaactta aaaacatttt ggggtgacat acatgaaagt
     2521 tgttgggttg aatcgtatgc gtgaagttga aactgaatta caacaacgct tttcagatgt
     2581 agattttaaa ttttataaaa aagcatcaga aatacctgag agcgacttgg ctaatttaga
     2641 tatattagtt ggttatgatg gcggtatcaa tgaggcattt ttacgacgtt gtacaaattt
     2701 aaaatggatc gcatggtttg caacgggtgt aaatacatta ccgttagatt atattgcaga
     2761 tcacggcata cttttaacta atggaaaagg tgttcaagct aaacaattat ctgaatacat
     2821 tttggctttc attttagatg attataaaaa gatgaaacta tcatatgata accaacgaca
     2881 acatatatat gattcgaaaa taactggtaa acgcctatca ggacaaacag ttttattttt
     2941 aggtacaggt gcaattgcta ctagaactgc gaagttagca aaggctttta atatgaattt
     3001 aattggtctg agcaagtcag gtcagaacaa agatgagttt gatgagatat atactatcga
     3061 atcacttgaa agcacattac caaatgctga cattgttata aatgctttac cagaaacgca
     3121 agaaacgatt catttactaa agaaaaaaca ttttgaatta atgaaagatg aagcactttt
     3181 tataaatata ggacgaggta gcatagttaa agaaacgctc ttaatagaag tattaaaaag
     3241 tagagttatt cgacatgcat atttagatgt gtttgaaaat gaacctttga aacctaatca
     3301 tgaattatat gaattggata atgtaactat aacagcgcat ataactggta atgattatga
     3361 agcaaagtat gacttattag atatttttaa aaacaatcta gttaattttc tcaataagaa
     3421 tggtctaatt gagaatgaag ttgatgctaa aaaaggctat taaatgaaat catcatgtaa
     3481 atattgacac gcgcgcaata ctacagttat atttatagta agtaataata attattatat
     3541 aagaaagatg gtgatataga tgagtgttga aatagaatca attgaacatg aactagaaga
     3601 atcaattgca tcattgcgac aagcaggcgt aagaattaca cctcaaagac aagcaatatt
     3661 acgttattta atttcttcac atactcatcc aacagctgat gaaatttatc aagcactttc
     3721 acctgatttt ccaaatataa gtgttgcgac aatatataat aacttaagag tgtttaaaga
     3781 tattggaatt gtaaaagaat taacatatgg agactcatca agtcgattcg actttaatac
     3841 acataatcat tatcatatta tatgtgaaca atgtggtaag atcgttgatt ttcaatatcc
     3901 acagttaaat gaaattgaaa gattagctca gcatatgact gactttgacg taacacatca
     3961 tcgaatggaa atttatggag tttgtaaaga atgccaagat aaataattta actttggtag
     4021 tatgacaaat taaaaaggcg ttagcttact tcatataagt aagcgtaata tttaagaagt
     4081 taaacgacat gaaagttgtt taacttcttt tttatgctca ttctgaaaaa tatttaaata
     4141 gaagaaaaga atattctaac tacggaagtg gtattaacta ataactagta tcctttataa
     4201 ataaacataa aaatatttaa acgaaggtct actaaataac tatccataaa gtgaagagaa
     4261 gtttgtttgt aaaactgaag taaaaagcgt aaggaattac actttaaata catatagtac
     4321 ttacgaatag aaaataatcc cttcaacagt aatttttaaa caaaaataag tgttgtttta
     4381 tgttgactct actagaataa cttgatatta tataaaagtc gtcaaacggc actaatattt
     4441 aaaaaacaaa tgttttaagt tgttgattta aaatattaat aaagtgtaaa tttgactatt
     4501 gaaattcgaa caaatacata ataaaataat atttgttgaa ttaattgagt taggaaattt
     4561 attttttaga aataaaataa ttaaaaataa ttcttgactt acaaaaactt gcgagttata
     4621 attaaatctt gtaagtgaca aacgaacatt gaaaactgaa tgacaatatg tcaacgttaa
     4681 ttc
//
LOCUS       DAPIDM010000167         4567 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000167 DAPIDM010000000
VERSION     DAPIDM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.169"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            208..1323
                     /locus_tag="RJE42_002434"
     CDS             208..1323
                     /locus_tag="RJE42_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV5877825.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     NKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSERQKIIRRNFK
                     CVLKHDFDGESLFRVQSLIYRDADFDIQLVYKLINLK"
     gene            1383..2309
                     /gene="pstC"
                     /locus_tag="RJE42_002435"
     CDS             1383..2309
                     /gene="pstC"
                     /locus_tag="RJE42_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDV5877826.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2311..3228
                     /gene="pstA"
                     /locus_tag="RJE42_002436"
     CDS             2311..3228
                     /gene="pstA"
                     /locus_tag="RJE42_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV5877827.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPTSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3275..4126
                     /locus_tag="RJE42_002437"
     CDS             3275..4126
                     /locus_tag="RJE42_002437"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDV5877828.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPTVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKENYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4133..>4567
                     /gene="phoU"
                     /locus_tag="RJE42_002438"
     CDS             4133..>4567
                     /gene="phoU"
                     /locus_tag="RJE42_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV5877829.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYNINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNAAS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTV"
BASE COUNT         1664 a          687 c          810 g         1406 t
ORIGIN      
        1 ttaaatttcg ataatttttc aggaagcaat ttaattttac taatgaagca atatttttta
       61 gattaacaaa aattaatatt tacattttct taacaaattt ttatgtaaca tttacagttt
      121 ctaaaaatga ggttaataat tcaaggttaa gataaagatg taatcaatac aaatactatt
      181 tgttgttcat acagggagga tatttcaatg aaaaaatggc aatttgttgg tactacagct
      241 ttaggtgcaa cactattatt aggtgcttgt ggtggcggta atggtggcag tggtaatagt
      301 gatttaaaag gggaagctaa aggtgatggc tcatcaacag tagcaccaat tgtggagaaa
      361 ttaaatgaaa aatgggctca agatcactcg gatgctaaaa tctcagcagg acaagctggt
      421 acaggtgctg gtttccaaaa attcattgca ggagatatcg acttcgctga tgctagtaga
      481 ccaattaaag atgaagagaa gcaaaaatta caaaataaga atatcaaata caaagaattc
      541 aaaattgcgc aagatggtgt aacggttgct gtaaataaag aaaatgattt tgtagatgaa
      601 ttagacaaac agcaattaaa agcaatttat tctggaaaag ctaaaacatg gaaagatgtt
      661 aatagtaaat ggccagataa aaaaataaat gctgtatcac caaactcaag tcatggtact
      721 tatgacttct ttgaaaatga agtaatgaat aaagaagata ttaaagcaga aaaaaatgct
      781 gatacaaatg ctatcgtttc ttctgtaacg aaaaacaaag agggaatcgg atactttgga
      841 tataacttct acgtacaaaa taaagataaa ttaaaagaag ttaaaatcaa agatgaaaat
      901 ggtaaagcaa cagagcctac gaaaaaaaca attcaagata actcttatgc attaagtaga
      961 ccattattca tttatgtaaa tgaaaaagca ttaaaagata ataaagtaat gtcagaattt
     1021 atcaaattcg tcttagaaga taaaggtaaa gcagctgaag aagctggata tgtagcagca
     1081 ccagagaaaa catacaaatc acaactagat gatttaaaag catttattga taaaaatcaa
     1141 aaatcagacg acaagaaatc tgatgataaa aagtctgaaa gacaaaaaat aataagacgc
     1201 aatttcaaat gtgtcttgaa acatgatttt gatggtgaat cattatttag agtacaaagc
     1261 ttgatttatc gagacgctga ttttgacatt cagttagtct acaagcttat caacttaaaa
     1321 tagtggttca tcattatttt acaaatctaa ttattttggg agtaatagaa agaggtttga
     1381 ttatgacttc atctactaat gttaaagctt taattgaaaa aaataataat aaaaaaggga
     1441 agcataatga caaaattata ccagttattt tagccgcaat ttcagcgatt tccattttaa
     1501 caacactagg tatattaatc acattgcttt tagaaaccat cacttttttc accagaattc
     1561 caataactga atttctattt tctactactt ggaatcctac cggttcagac cctaagtttg
     1621 gtatctgggc attgataata gggactttaa aaatcacagt tattgcgact atatttgcag
     1681 ttccagtcgg gcttggtgca gcaatttatt taagtgaata tgcaagtgac cgtgcacgac
     1741 gcataattaa accgatatta gaaattttag caggaatacc aacaattgtg tttggtttct
     1801 ttgcactaac ctttgttaca ccagtattaa gatctttcat accaggtctt ggagaattta
     1861 atgctataag tcccggctta gttgtcggta ttatgattgt ccctctcatc acaagtttga
     1921 gtgaggatgc aatggcatct gtaccaaata aaattcgaga aggtgcctat ggacttggag
     1981 caactaaatt agaagtagca actaaagtcg tacttcctgc agcaacatca ggtattgtag
     2041 cttcaatcgt tctcgcgatt tcaagagcaa ttggagaaac gatgattgta tcattagcgg
     2101 cagggagttc gccaacagct tcattaagtt taacaagttc aattcaaaca atgactggat
     2161 atattgttga gatagcgaca ggtgatgcaa catttggatc aaatatttat tacagtattt
     2221 atgctgtagg attcacatta tttatcttta ccttaatcat gaatttactt tctcaatgga
     2281 tttctaagcg ttttagggag gagtattaat atggaaacga cagataataa tagacaatca
     2341 cttgtcgatc aacaacttgt ccaaaaacat ttatcatcca gaacggttaa aaataaagtg
     2401 ttcaaactca tatttttagc atgtacatta ttaggacttg tcgtacttat tgcgttgtta
     2461 actcaaacat tgattaaagg ggtaagtcat ttaaatttac agttttttac taatttttct
     2521 tcttcaacac catctatggc tggcgttaaa ggcgcgttaa tcggttcact ttggttaatg
     2581 ttaagtatca ttccattatc aatcatccta ggaataggta cagctatata cttagaagaa
     2641 tatgcgaaaa acaacaaatt tactcagttt gttaaaatca gtatttccaa tttagctggt
     2701 gtaccatcag ttgtatttgg gctattaggt tacactttgt tcgttggtgg tgccgggatt
     2761 gaagccttga aaatgggtaa cagtatattg gcagcagcgc taacaatgac cttactgata
     2821 ttaccaatta ttattgtttc aagtcaggaa gcaattagag ctgtacctac ctcagtacgc
     2881 gaagcttctt acggcttagg tgctaataaa tggcaaacga taagacgagt tgtcttacca
     2941 gcagcgttac ctggtatttt aactggattc attttgtctc tttcaagagc actgggagaa
     3001 acagcaccac ttgtgcttat cggtatacca actatattat tggcaacacc tagaagtata
     3061 ttggatcaat tttcagcatt acctatccaa atatttactt gggcgaaaat gcctcaagaa
     3121 gaattccaga atgttgcatc ggcaggcatt atcgttttac tagttatctt aatcttaatg
     3181 aatggcgttg cgattatttt acgtaacaaa tttagtaaaa aattctaatt taaacaatca
     3241 atctcattta tctattaaaa agggagtttt aaatatggcg caaacacttg cacaaactaa
     3301 acaaatatct caaagtcata cgtttgatgt ctcacaaagt catcataaaa caccagatga
     3361 tacaaactca cattctgtta tatattcaac acaaaattta gacttatggt atggcgaaaa
     3421 tcatgcattg caaaatatta atttagatat ttatgaaaac caaatcactg ccattatagg
     3481 tccatctggt tgtggtaaat caacatatat taaaactttg aatcgaatgg ttgagttagt
     3541 acccacagtc aaaacagctg gtaaaatatt atatcgcgat caagacattt ttgatcaaaa
     3601 atattctaaa gaacaattac gtacaaatgt gggcatggtc tttcaacaac ctaatccatt
     3661 tccaaaatca atatacgata atattactta cgggccaaag attcacggta ttaaaaataa
     3721 aaaagttctt gatgaaatcg ttgagaaatc attacgtggc gctgcaattt gggatgaatt
     3781 aaaggatagg ttgcacacaa atgcatatag tttatccggt gggcaacaac agcgtgtttg
     3841 tatcgcgcgt tgtttagcaa ttgaacctga agtcatttta atggatgaac ccacatcagc
     3901 attagatcca atctcaacat taagagtaga agagttggtt caagaattaa aagaaaacta
     3961 tacgattatt atggttacac ataatatgca acaagctgct cgtgtatcag ataaaactgc
     4021 atttttctta aatggatatg tcaatgaata cgatgataca gataaaattt tctctaatcc
     4081 atctaataaa aagacagaag attatatttc aggaaggttt ggttgatata taatggcaat
     4141 aattagacaa cgatatcagg agcaacttga tgatttaata aaagaattac gtcggttagg
     4201 tgcaaatgtc tatgtgagta ttgaaaatgg tataaaatca ttaagtattg acgatagagg
     4261 ctttgcacga caaacagtta aaaacgataa acatatcaat caactaaatt ataatattaa
     4321 tgagcgagtt atcatgttaa ttacaaagca acagcccatt gcgagtgatt tgcgtatgat
     4381 gatttcttca ttaaaaatcg cctccgattt agagcgtatc ggtgataatg cagcgagtat
     4441 tgccaatatt cgattgcgta cgaagattac agatgattat gtgttaaccc gtttaaagac
     4501 aatgggtaaa ctagctatgt taatgttaaa ggatttagat caagcattta aaaagaaaga
     4561 taccgta
//
LOCUS       DAPIDM010000168         4493 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000168 DAPIDM010000000
VERSION     DAPIDM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.170"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..260)
                     /locus_tag="RJE42_002439"
     CDS             complement(<1..260)
                     /locus_tag="RJE42_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDV5877830.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVERQYEGFFVSKVSILDEPY"
     gene            564..4004
                     /locus_tag="RJE42_002440"
     CDS             564..4004
                     /locus_tag="RJE42_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDV5877831.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKCKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQLATDNARDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYRDESYITLKSQSQLLDEINAVVEYQDMSYSTIEEFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVNLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMVTEFETNKADITRRQQRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKKLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     REQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4024..>4493
                     /locus_tag="RJE42_002441"
     CDS             4024..>4493
                     /locus_tag="RJE42_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDV5877832.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFTAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKG"
BASE COUNT         1740 a          648 c          732 g         1373 t
ORIGIN      
        1 ttatatggtt catcaagtat gctaacttta cttacaaaga atccttcata ttgtcgttca
       61 acttgatgta ctgcatcatt taatgtttga tgcgttttga catcactgtc accaaaaaac
      121 tcattcttat aaatattatt ttcaacttct ggaaagaaca aataaccaat gcccgaaatg
      181 gttaaagtga ttaacagtgg agcaataaat attgctgcgt agaaatgtaa tctttgtaat
      241 ggattaaatg tatttttcat atttccctcc caattggcta ttatacggtg tcaattctgt
      301 gacgtgtgtg aacaaactgt gacaatattt attttctaga aaaatttaac gttgatttgt
      361 gatttttaga aaaatgaact tttaagttgt aatgtttgaa gaaaattgat tattcgtatg
      421 ttttatcaag cagctatgat aaaatttaaa cataatacaa tgcgagccat tcaacgacct
      481 ttgtttaaat ggacatcgat attgtattaa ttcgttttaa caagcaagca tttctatgtg
      541 aacgaaccaa aggggaaagt aacatgatta ataaagaaca attagatctt ttatataaat
      601 taaaaaaaga agttgaaaag tcgcgaaatg aagcactttt acatacaatt aaccaagtaa
      661 ttaagaaagt atatttgcag caatatacat gttcgttcgt tggacatttt tctgcaggta
      721 aatcgacact gataaattta ttaattgaac aagatatctt accaagttca cctgtaccaa
      781 cgacaagtaa tactgctatt gtgtcagttt cagacaatca cgatattatt gctaatttgc
      841 cgaatcaaac gtatgccaaa ttatctaatt atgatgaagt aagggaaatg aatcgccaaa
      901 atgtcgacgt tgaatctgta gaaattaatt ttcaatcagc taaatttgaa aatgggttta
      961 cgttgcaaga tacaccaggt gttgattcaa atgttgcatc acatcagtca ataacagaac
     1021 aatatatgta tacaagtaat atgatatttt atacggttga ctataaccac gttcaatctg
     1081 aacttaactt taagtttatg aagcatataa atgatgttgg aatacctgtt gtgtttatca
     1141 ttaatcaaat tgacaagcat caagacgatg aattgtcatt ctctacgttt aaatcacgag
     1201 ttgaaaaatc aattgcagat tggggcatta aattagaacg caccttttat gtatctaaat
     1261 ttgatcaccc tgaaaatgaa cttgaagtat tatcaagtta tctaatttca ttagatcaac
     1321 atagagagac aatagaggat tatacatcaa gaacggttga atatattacc gaagctcagc
     1381 tagattacat tcagtctgaa atccaggaag tactagaaga tttaggtatc gaagaagcag
     1441 agtttgaaca agcattttta aatagtcaac aacatcaagc aattagtgaa gaggcacaac
     1501 ttttaaataa tccagatgaa ttaatggcat ttttaaagaa taagtgtaaa aatattttag
     1561 aaaatgcata cattatgccg cataatatga gagaaatgtt acgaagttat ttggaaagta
     1621 tgtctcaaga ctttaatgtt ggcggatttt ttaataaaaa gaagaaaaag ctacaaattc
     1681 aacaacagcg attattaaca gcgacagatg cgttacaaga acatgttaat caacaaattc
     1741 gtcaaccaat gcgagaagac atgtcatttg ttacgcgttt tatcaataaa aaagaagctt
     1801 cagataaagt attaaatcag cattatgacg ttaagccgga aatgattgaa ggtttatatc
     1861 aaccacaaac atcaatcagc aatacttatg tacttacatt ttcagacgaa gtggttaaag
     1921 ccattaagaa atatgttgaa caacaatcaa caccaatttt taaagaaata atagaaaatg
     1981 tgcaggcaga tgaattacca acagaagaaa gtgatgattt aaaagaatat caacgttata
     2041 cagaattaaa tgagctgcgt cagtcattga cgactaaaaa ttatcgtcac tactatattc
     2101 atttagatga atctctagat aaattaatag gtcgacaaga gacaacatat caactggcta
     2161 ctgataatgc tcgggataat cgtgataatc agcagctaaa tcaaaataca gctacaacaa
     2221 atatgtctat agatattcaa aaagcgcttg atataatttc ggatgtgcct ttgttcaagc
     2281 gtacaaagca agatatccac gaaacattaa cacgtataga taataaatta ataaaaatag
     2341 gtgtatttgg aacatttagt gctggtaaaa gtagtttgat aaatgcatta ttaggcgagc
     2401 atattttagt cagttctcca aatcctacta cagcagctac tacagaaata tcttatagag
     2461 acgagagtta tataacgcta aaatcacaat cgcaattatt agatgaaatt aatgcagtag
     2521 ttgaatacca agatatgtct tattcgacta tagaagaatt tattaattca gatttagaaa
     2581 agttaaaatc acatttaaat aaaaatcaac tcgcttttat tcaggcagtt gaaaaacatt
     2641 ataaattgta tgtcaatatg ttggaaaatg gagaaaaaca tgccattaat caacaggaat
     2701 tgaaaaagtg gagtgcagaa gatgaatacg caacatttgt taaaacagta cacattgcat
     2761 taatgcatga ttggttaaaa ggtaaaataa ttgttgattc attagggcta cactcaaata
     2821 accaaaggca tacaaatgaa accgagcaaa ttttaacttc ttcagactta atcttgtatg
     2881 taagttattt taatcattcg tttactgata atgacaaggc gtttatagaa catatgaaag
     2941 atatgaacca gttgaatgaa aaccaagcat ttaaaatggt aattaatgct gctgatttag
     3001 cagaaagtca agatgatctt gaagcggttg aaacatatgt atcagatgca ttaagacaag
     3061 taaacttaca atcagacatt tttgctgtat caagtcgaaa tgcattgcaa gctgaagata
     3121 agggcattga tcaattaaaa caaagcatac aacaatttgt tgatgttgaa tctaaatcaa
     3181 ttttagaaca acaaatgatt catcagcttc aacaaatgga tcgttcttat gtagagatgg
     3241 ttacagaatt tgaaacaaat aaagctgata ttacacgtcg ccaacaaagg ttaacagtct
     3301 ataaagataa acagcgttta caacatcaat taattgatgc gacgctacaa catacagaca
     3361 acgaagttga agaacaagtt tatcatttaa atgcacgttt aaaattacaa ctacttgacg
     3421 atgttaaatc agtgtttaac tctcaaatga cgcaaaatag tgattttaat gaagaaaaga
     3481 aagtgtctac gagagtatac ttagatcaaa ttcatcaacg attgttttta gagcaatctt
     3541 taattacaga acgtataaaa aaatacttta ataagaaact tactgagcaa attgcaccaa
     3601 tcgttcaaca attagcagat ttacatgtca ttgttaatcc tcagtttaac tttgaatcag
     3661 ctaatataga gcaaccatta ttgcacatcg atttcaacga tatgctaaat gcattgccta
     3721 aacaattaac aaaacgtaaa attttgaatc caaatgggca aagagatata catgaatcaa
     3781 tttgtcaaag tacgttagga ttattacaac cacaaatggg attattgagg gaacaacttg
     3841 aattatatgt aaagcaaatg gctgtagaag ctgaatcgca atttgaaagt tttgaagcta
     3901 atattcaaac acaaataaac gatttattag catttgattt agatacaaca cttatcaatc
     3961 aattgaaaga taaacatcaa caactgaaaa ctattttata ttaagaaaga aggaacgttt
     4021 taaatgccta ataaaatatt acttgtagat ggtatggcgc tattatttag acatttctat
     4081 gctacaagtc ttcataaaca atttatgtac aattcacaag gtgtacctac aaatggaata
     4141 caaggatttg tacgtcatat ctttacggca atacatgaaa tacgccctac acatgtagct
     4201 gtatgttggg atatgggaca atcaactttt agaaatgata tgtttgatgg ttataagcaa
     4261 aatcgttctg caccaccaga agaattgata ccacaatttg attatgttaa agaaatttca
     4321 gagcaatttg gctttgtaaa tattggcgtt aaaaactatg aagcggatga tgttataggt
     4381 acattagcac aacaatattc aactgataac gatgtctata ttattacggg cgacaaagat
     4441 ttactgcaat gtattaatga caatgttgaa gtttggctaa ttaaaaaagg ttt
//
LOCUS       DAPIDM010000169         4466 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000169 DAPIDM010000000
VERSION     DAPIDM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.171"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(136..705)
                     /locus_tag="RJE42_002442"
     CDS             complement(136..705)
                     /locus_tag="RJE42_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDV5877833.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRHGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            915..1133
                     /locus_tag="RJE42_002443"
     CDS             915..1133
                     /locus_tag="RJE42_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDV5877834.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1214..2200)
                     /locus_tag="RJE42_002444"
     CDS             complement(1214..2200)
                     /locus_tag="RJE42_002444"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDV5877835.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNAPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2399..2575
                     /locus_tag="RJE42_002445"
     CDS             2399..2575
                     /locus_tag="RJE42_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDV5877836.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2590..3192
                     /locus_tag="RJE42_002446"
     CDS             2590..3192
                     /locus_tag="RJE42_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDV5877837.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            3462..3569
                     /locus_tag="RJE42_002447"
     CDS             3462..3569
                     /locus_tag="RJE42_002447"
                     /inference="COORDINATES: protein motif:HMM:NF028245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDV5877838.1"
                     /translation="MISIANALHLMLSFGMFIVTFIGVVVAIINLNNKK"
     gene            4204..>4466
                     /locus_tag="RJE42_002448"
     CDS             4204..>4466
                     /locus_tag="RJE42_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDV5877839.1"
                     /translation="MKNKIFGAILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVW
                     SYYYHGHKGHGATAIGKYRSFSGYTRAGVKAKASATKNNWWVN"
BASE COUNT         1511 a          619 c          680 g         1656 t
ORIGIN      
        1 tgtatgtgtt tgaatatcaa gcacaaatat tattataaca agattaagtt aatttgcaat
       61 agatacctta aaaaaatatg cagaacattt acactatatt tataaaacat tccgcatatt
      121 ttaaaaataa acaatttatt tatcttcaaa taaagaatag cgtgtcaatg cttcaaacac
      181 attcaatgtg gctttattat aatctattgg ttggtcggga ttttttgata tcatgcgaga
      241 ttgtttatct accatgtaat agtaaaaaat ggaattgtta tattgatgcc ataaactgtg
      301 gtatgaaaca tgaagaatga ttgattcatt attaataaat gtcactttac atttacgatt
      361 ttttaattct ttgatacttt cgatataatg catatttaac caaatatttt cattttgtct
      421 gtcagaatgt gttgggaaaa aataagttgg aaataatggt gttagtaaaa taggtggttt
      481 actagaaatg ccagtaatgc ggtttgtctc tgctttcttg ccaagataag tattaccata
      541 aaatttgcaa gagcgttcaa tgattttctg gactttaaaa ggattttgaa tacgcgtttt
      601 gtcaaaccga attatttcac taccatgtct ttgatcgtca tcatcaaatg ctggtcgaat
      661 aaccatgtct cctttgcgta tcacataaat attttgagaa tacataagca aaacctcgct
      721 ttattaagtt ttaaaccatt aatataaaaa tacaattaat gatataaaga tattttataa
      781 taaaattctg agttgtcaag ttattatttt ggtgaaaatg tacaataatt tttaactaat
      841 agtcagaata ttcattgatt tcaaaattag aaaaatgtat acttattata aattaaagaa
      901 aggtagtggt ttctatgaaa tacaatacta atgttaaaca tacaacttta gaagcgtttg
      961 ttacaaccgt caatgatttg ggtattgaat taattatcaa tgaagcactt cgagaggtaa
     1021 gaaaacgaca gctcatagaa cttatagatg acgcactcgt caataaagat gaagcagcat
     1081 ttaatcaata tacggcagaa tacaaaaatt tggaggcatt tctcggtgaa taacattgat
     1141 tcgaattcca taacgcaatc ttaaaaggac tattcttaac atttcataca tgtaagaata
     1201 gtcctttaat cgtttatgac attaatctaa ttaattcatg tctatctatg tcaccgaaat
     1261 agtgatataa atcatattct gatagtgctt cttctatgtg ctcaaagtcg tgtagacagc
     1321 caactaatgc attttcaaga tcagtgacat ctccgacacc aaagaaatca ccgaaaattt
     1381 ttgcatgttc gattttacct cgtttaacat caaacttgat ttgtacaaat cctttttcaa
     1441 atttttcctc gcgttcaaag ttatatttag ggtttctacc ataattccat tcccaagttc
     1501 tatatttgtc gttacttaac ttttcaatat tttcccaatc ttcatccgtt aatttatatt
     1561 cttctacttc agtttctcca aagatagttt tcaagatgat ttttttaaat tcttcaattt
     1621 ctaaaggggc atttaaaaat tcttgaatgt ttgctactcg tttacgaaca gatttaatac
     1681 cttttgattt aatcttagct ggattaactt ttagtgcatt ctgaacttcg tctaaatcac
     1741 tatttaacat caacgtacca tgactaaaca ttctattttt aactttaacc atggcatttc
     1801 ctgagatttt cgcttgtcca acttgaatat cgttacgacc agttaattca gcatttacgc
     1861 ctaaagattg taatgcttga acaattggtt cagtgaattt ttggaaattg tggaaactgt
     1921 taccatcatc atctgttata aaactaaagt ttaaatttcc agtatcatga taaacagcgc
     1981 caccaccaga aattcttcta actacatcga tgttatgagc atctatatat gtctgattta
     2041 cttcttctat cgtattttga ttctttccaa caatgataga tggtctattt atgtaaaata
     2101 aaaagtaact ttcttctgct ggtaaatttt ttaaaacata ttcttccatt gctaagttta
     2161 aagttggatc tgtaatatta ttattactaa tgaatttcat tacaatctct cccttatatc
     2221 tatatatatt ctctacttac ttatgcctta actttgctga aaatgccaat tatatactca
     2281 caaatcgttc taaatatcgg cttaaagtta cattttatta tttttgattg ttaatagttt
     2341 atttgttaag tgaattttag tacaatataa tttagttatg atttaaggag gacttaaaat
     2401 gactgttgca gaagtgggta acattgttga gtttatggat ggattaagag gtcgtgttga
     2461 aaaaatcaac gataactctg ttattgttga cttaacaatt atggaaaatt ttaatgacct
     2521 tgatttaccg gaaaaaactg ttatcaatca taaacgatat aagattgttg aataagaagg
     2581 taagttataa tgaataaaat ctcgaaggct ttaacttggt ttattataag tttcattata
     2641 tttcatctca tattatttat tatgtggggc gaacaccaag aatactggta tttatataca
     2701 ggtataatgc taattgctgg tatcagttat gtgttttatc aaagagatat tgaatctaag
     2761 cggttgctta catcaattgg tgttggtatt attacggcaa ttattttaat tatgcttcaa
     2821 cttttattct cacttataac ttctaattta agttatagtt cattaattaa agaattagca
     2881 agaacaggtg tcaattggaa gtggcaaatg ttagtaactt tactttttgt cattccatgt
     2941 cacgagttat atatgagaac tgttttacaa aaagaattaa cacatttttc aataccgaaa
     3001 tgggtagcta tattaataac tgcaatttgt tctagttcat tatttattta tttagataat
     3061 tggtggatag taacatttat atttgttgca caagtgatat tatcattaag ttatgagtat
     3121 actcgacgta tagcaacaac atccgttgca caaattgtag ctattatatt gttattaatt
     3181 tttaatgctt aaataagctt ttcttagacg tctgtgttct aagaaaagct tttttgcttt
     3241 agaatagcaa tacgaagatt cgaggcatgc ttcattatag tgtacacgtt tttaacggca
     3301 agctgttatt attgagatta ggttttagtc agtttttatt actaaatatt ttataaaaat
     3361 ggtataatat ctttcgagca agttgaattg atggtggcta tctgagtaaa gggggtggtg
     3421 cctatggcat tactgaacac ttttgaaagg aaaagcctat tgtgatatct attgcaaatg
     3481 cattacattt aatgttaagt ttcggtatgt ttatcgtcac tttcattggt gtagtagtcg
     3541 caataattaa tttaaacaat aaaaaataac catcatttca aaactttgac aacgaatgat
     3601 ggttaaagca aatctcattt agtcaccgtc tttttaacgg gctcattagg cgacatgtta
     3661 ctgcatgtcg tctttttaca tttttatagt aacatatctt ttatttaatt gtctatgttt
     3721 attagatgtg taacaaaatt ttgaagtatt tttcgtatat tgcctgctaa acatgtcaga
     3781 tttgaaagga aaatcatact gtggtagaaa tcaattatga ttatatggta gtacttataa
     3841 ataagtgatt tctattgaat atgtaatgaa gttcatatat taataaaact ttcattggta
     3901 tagtagtcgc aataattaat ttaagcaata aaaaataacc atcatttcca aactttgaca
     3961 aagaataatg gttaaagcaa atctcattta gtcaccgtct tttaacgggt tcaataggcg
     4021 acatgttcct gcatatcgtc tatttatgca ctgcgcaatt taataataat acaattatac
     4081 tttgatgtcc atttaacata aaatactgga aatgtagaaa ggtaaattca tcttgaaata
     4141 ttcagaaaat tctgtataat ttaaataaag agatatagat taaatataaa gaggtgtttt
     4201 taaatgaaga acaaaatttt tggcgctata ttatatttat ctttagcact tggattatca
     4261 acagcagctt atgcatctac agaatacgca gaaggaggca cttggagtca cggtgtcggc
     4321 agtaagtatg tttggtcgta ttattatcat ggtcataaag gacatggtgc aacagctatt
     4381 ggaaaataca gatcatttag tggttataca agagctggtg taaaagcaaa agcatcagct
     4441 actaaaaata attggtgggt caatag
//
LOCUS       DAPIDM010000170         4394 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000170 DAPIDM010000000
VERSION     DAPIDM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.172"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..656
                     /locus_tag="RJE42_002449"
     CDS             <1..656
                     /locus_tag="RJE42_002449"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDV5877840.1"
                     /translation="SYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQNE
                     DEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTE
                     IKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDD
                     CEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS"
     gene            643..2316
                     /gene="menD"
                     /locus_tag="RJE42_002450"
     CDS             643..2316
                     /gene="menD"
                     /locus_tag="RJE42_002450"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV5877841.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASAIRHILNKKKGL
                     IIVGDMQHQEVEQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLMG
                     DTTVNRVNWLEKWQSLEKKGRQEIENYLEQATDESAFVGELIKKTFEEDALFISNSMP
                     IRDIDNLLLNKNIDIYANRGANGIDGIVSTALGMAVHKRVTLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATVYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            2303..3106
                     /gene="menH"
                     /locus_tag="RJE42_002451"
     CDS             2303..3106
                     /gene="menH"
                     /locus_tag="RJE42_002451"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV5877842.1"
                     /translation="MTHYNYYEANVETNQVLVLLHGFLSDSRTYHNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDQSITMLGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQQ
                     GLPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            3099..3920
                     /gene="menB"
                     /locus_tag="RJE42_002452"
     CDS             3099..3920
                     /gene="menB"
                     /locus_tag="RJE42_002452"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV5877843.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(4158..>4394)
                     /gene="sspC"
                     /locus_tag="RJE42_002453"
     CDS             complement(4158..>4394)
                     /gene="sspC"
                     /locus_tag="RJE42_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDV5877844.1"
                     /translation="SNHQLQKSICIRHGDDTSHNQYHILFIDTAHQRIKFSSFDNEEI
                     IYILDYDDTQHILMQTSSKQGIGTSRPIVYERLV"
BASE COUNT         1537 a          633 c          857 g         1367 t
ORIGIN      
        1 acagttatat atttttgtta gaatcacaag attctatatt cttttcacaa acacctgaac
       61 aattaataaa ggtcaataat aaaatactat cgactaaagc tgtagcaggt acaattaaac
      121 gttcacaaaa tgaggacgaa gatacaaaaa atgttgaagc atttttaaaa gataataaaa
      181 acttaatcga acatcgattt gttgttgaca gtattttaca tgatattaaa ccttatatca
      241 ctgaattaca ttatgataag acgcctaaaa ttctaaaaaa tgatcattta tatcacttgt
      301 atactgaaat taaggcaccg cttaaagatg attcatatat aagtttaatt gatcatttac
      361 atccaacacc tgcattaggt ggatatccaa aagaatttgc aatggatttt attgagcaga
      421 aagaatttgg tacacgagga ttatatggtg cgccggttgg ctatatagat atatatgatg
      481 attgtgaatt tattgttgca attcgttcga tgcttattaa gaaagcacaa gcaactttat
      541 ttgctgggtg tggcattgtt aaagattctg atccagatag tgaattggca gaaacgaacc
      601 ttaagttcac acctatgatg aatgcattag gagtcgatat gaatgggaaa tcataaagca
      661 gctttaacga agcaagtttt tacatttgca tctgagttat atgcgtacgg cgtaagggaa
      721 gtagttatca gtccgggatc acgctcaacg ccacttgcac ttgcatttga agcacatcca
      781 aatattaaaa catggataca ccccgatgag cgaagtgcag cgttttttgc agttgggtta
      841 attaaaggca gtgaaagacc tgtcgctata ttatgtacgt caggtacagc agcagcgaat
      901 tatacgcctg caattgctga aagccaaatt agtagaattc cattaatcgt tttaacaagt
      961 gaccgtccgc atgaattaag aagtgtaggc gcaccacaag cgattaatca agtaaatatg
     1021 tttaataatt atgtaagtta tgagttcgat atgcctattg cggatgatag taaagagacc
     1081 attgatgcaa tttattatca aatgcaaatt gctagtcaat atttatatgg accacataaa
     1141 gggccgattc attttaactt gccatttaga gatccgttaa cacctgattt gaatgcaaca
     1201 gaattgttaa cttctgagat gaagatttta ccccactatc aaaaaagtat agatgcatcg
     1261 gcaataagac acattttaaa taagaaaaaa ggtttaatta ttgtagggga tatgcagcac
     1321 caagaagttg agcaaattct gacgtattca acgatatatg atttgcctat tttagctgat
     1381 cctttaagtc atttaagaaa atttgatcat ccgaatgtta tctgtacata tgatttgttg
     1441 tttagaagcg gtttagactt aaaagtcgat ttcgtaattc gtgttgggaa accagtgatt
     1501 tctaaaaagt taaatcaatg gttaaagaaa actgatgcat ttcaaatatt agtgcaaaat
     1561 aatgataaga ttgatgtctt tccaatagca ccagatattt cttatgaaat atctgcgaat
     1621 gatttcttta ggtcattaat gggagacacg acagttaatc gtgtcaattg gctagaaaaa
     1681 tggcaaagct tagagaaaaa agggcgccaa gagattgaaa attatttgga gcaagctaca
     1741 gatgagagtg cattcgttgg tgaattgatt aagaagacgt ttgaagagga tgctttgttt
     1801 attagtaata gtatgccaat cagagatatt gataatttgt tattaaacaa aaatatagac
     1861 atctatgcaa atcgcggcgc aaatggtatt gatggtatcg tatcaacagc gcttggtatg
     1921 gctgtgcata aacgagttac attattgata ggtgatttgt cattttatca tgatatgaat
     1981 ggactattaa tgtcaaaatt aaataatatt cagatgaata ttgtattatt gaacaacgat
     2041 ggtggcggta ttttttcata tttaccacaa aaagaaagtg caaccgtcta ttttgaacgg
     2101 ttgtttggca caccgacggg attggatttc gaatatacag ctaagttata tcaattcgat
     2161 tttaaacgtt ttaacagtgt ttcagaattt aaaaatgcca cattgttatc tgaaacttcg
     2221 acgatttatg aattgataac gaatcgcgaa gataacttta aacagcatca aattttatat
     2281 cagaaattga gtgaaatgat tcatgacaca ttataattac tatgaagcaa acgttgagac
     2341 caatcaagtt ttagtattac tgcatggttt tcttagcgac agtcgtactt atcataatca
     2401 catcgataaa tataccgata tctgtcatgt catcactata gacttaccag gacatggcga
     2461 agatcagtct tcaatggatg aaacgtggaa ttttgattat attacgacgt tgttagatcg
     2521 aattttagat aaatataaag atcaatcgat aacaatgcta ggttactcca tgggtgggcg
     2581 tgttgcatta tattatgcaa ttaatggtca catccctata tctaatttga tattagaaag
     2641 tacgtcacca ggtattaaag aagaagcaaa tcaactagaa cgtcgtcttg ttgatgatgc
     2701 acgtgctaaa gtattagaca tagcaggtat tgaattattt gttaatgatt gggaaaagtt
     2761 gccattattt caatcgcaac aaggtttacc agttgaaata caacatcaaa taagacaaca
     2821 acgattgtct caatcgccac agaaaatggc caaagcatta agagattatg gtacaggtca
     2881 aatgccaaac ttatggccgc gcctgaaaga aattaaagta ccaacattaa tattagctgg
     2941 agaatatgat gaaaaatttg tacagattgc gaaaaaaatg gcaaatttaa ttcctaatag
     3001 taaatgtaaa ttaatttctg ctacaggtca tacaattcat gtggaagata gtgatgaatt
     3061 tgatacaatg atattaggat ttttaaagga ggagcaaaat gactaacaga caatgggaaa
     3121 cacttagaga atatgatgaa atcaaatatg aattttacga agggattgct aaggtaacaa
     3181 taaatcgccc tgaagtacgc aatgcgttta cacctaaaac agttgctgaa atgattgacg
     3241 cattttcacg tgcacgtgat gatcaaaacg tttcagttat cgtattaact ggtgaaggtg
     3301 atttagcatt ctgttctggt ggtgaccaga agaaacgtgg acatggtggt tatgtaggtg
     3361 aagaccaaat ccctcgctta aatgtattag atttacagcg tttaattcgt attattccaa
     3421 aaccggttat cgcgatggta aaaggttatg ctgtaggtgg cggtaatgta ctaaatgttg
     3481 tttgtgactt aacgattgct gctgataatg ctatttttgg acaaactggt cctaaagtag
     3541 gttcatttga tgcgggttat ggttcaggat atttagcacg tatcgttgga cataagaaag
     3601 cacgtgaaat ttggtactta tgtcgtcaat acaatgcaca agaagcttta gatatgggtc
     3661 tagtaaatac agtggtacct ttagaaaaag ttgaagatga aactgtacaa tggtgtaaag
     3721 agattatgaa acactcacca acagctttac gattccttaa agcagctatg aatgctgaca
     3781 cagatggttt agctggttta caacaaatgg ctggggatgc aacattgctt tattacacaa
     3841 ctgatgaagc gaaagaaggc cgtgatgcgt ttaaagaaaa acgtgatcct gacttcgatc
     3901 aattccctaa attcccataa gttattttga atatgatatt aagtcacttg cgtcgtatat
     3961 tagcgacgat aggtggcttt tttattttat aagaatttaa aagggcattt taattaatta
     4021 tcataaagat aagatagttt gagaaaatta atagagaaag aataaatgtt aaagattatt
     4081 attacaatga aaaaacgagc tggtgagaaa ttgaattctc gccaacccgc tttatgaagt
     4141 gaaaccattt aaattagtta tactaagcgc tcataaacga ttgggcgcga agtaccaata
     4201 ccttgtttgg atgacgtttg catgaggata tgctgtgtat catcataatc aagaatataa
     4261 atgatttctt cattatcaaa agatgaaaat ttaatgcgtt gatgtgccgt atctataaaa
     4321 agaatatgat attgattgtg acttgtatca tcgccatgac gtatacaaat tgatttttgt
     4381 aattgatgat tact
//
LOCUS       DAPIDM010000171         4373 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000171 DAPIDM010000000
VERSION     DAPIDM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.173"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            39..1676
                     /locus_tag="RJE42_002454"
     CDS             39..1676
                     /locus_tag="RJE42_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDV5877845.1"
                     /translation="MDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRG
                     KFGLGEGRLNKFTIEKLALGLSRYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANH
                     QITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDAS
                     ELVSRYIEDVGDPLQIDISFSKHNSSYIKPLPKSVAENYIKHIQNMIGYIPKSDLQVV
                     FTSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELA
                     NKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMI
                     QSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPF
                     VRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKK
                     IESIMTHFRSHPPQEIQGLKVKAIEDYLTSEVHQLDKDTTSQINSPKSNVIRVLFDEG
                     FIALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            1960..2226
                     /locus_tag="RJE42_002455"
     CDS             1960..2226
                     /locus_tag="RJE42_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877846.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            2370..2678
                     /locus_tag="RJE42_002456"
     CDS             2370..2678
                     /locus_tag="RJE42_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877847.1"
                     /translation="MTVNIVVSIVFCISMVVLSIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFALCISLSAVFLMSAILCFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(2804..3670)
                     /gene="galU"
                     /locus_tag="RJE42_002457"
     CDS             complement(2804..3670)
                     /gene="galU"
                     /locus_tag="RJE42_002457"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV5877848.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(3850..>4373)
                     /locus_tag="RJE42_002458"
     CDS             complement(3850..>4373)
                     /locus_tag="RJE42_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HDV5877849.1"
                     /translation="NYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPEVPSE
                     PETPTPPTPEVPSEPGEPTPPTPEVPSEPETPVPPTPEVPSEPGKPVPPAKEEPKKPS
                     KPVEQGKVVTPVIEINEKVKAVAPTKQKQSKKSELPETGGEESTNKGILFGGLFSILG
                     LALLRRNKKNHKA"
BASE COUNT         1354 a          757 c          706 g         1556 t
ORIGIN      
        1 taagtctaac tgagattttg aaaggatgtt tagcaacaat ggataaagaa ttatggatag
       61 aaagagctaa tgatagttta gttaaacatt tttatgagca gcaatctgac attgaacagc
      121 gagaaggttt tgaaagtaaa ttaacgtttg gtactgcggg tatacgcgga aaattcggtc
      181 ttggtgaagg tcgacttaat aagtttacta ttgaaaaatt ggcattaggt ttatcgcgtt
      241 atttaaatgc acaaacaaac aatccaacaa tagtcattca ttatgatatt agacatcttt
      301 caactgaatt cgcccaaatt attgctaatg tattagcaaa ccatcaaata acagtttatt
      361 tacctgatac atataaaacg acaccggaat tatcgttcgc ggtgcgcaat cttaatacta
      421 ctgctggcat tatgattaca gcaagtcata atccgaaaga ctataacggc attaaagtat
      481 atggttctga tggcgcgcaa ctatcaacag atgcatcaga acttgtaagt cgctatattg
      541 aagacgttgg cgatccatta cagattgata tatctttttc aaaacacaat tcatcgtata
      601 tcaaaccgtt acctaaatct gtagcagaaa attacattaa acacattcaa aatatgattg
      661 gctatattcc taaatctgat ttacaagttg tgttcactag tttgcatggt acaagtgttc
      721 caattgtacc tgagttatta aaatctttga atttcaatca atttaatctt gtagaagcac
      781 aatgtaaacc tgatccgaat tttagctcag ttcaaagtgc taatcctgaa gatcatcgcg
      841 cttttgatca agcagtcgaa cttgcaaata aaagtcatgc agacttatta atatcaactg
      901 accctgatgc ggatcgtctt ggcattgctg aacgtgatgc acatggtcat atcacatatt
      961 ttaatggcaa ccaaattggt gcgttgttac ttaattatcg tattcaacaa acgtctcaat
     1021 tacgtcatcg cttaatgatt caatctattg taagtagtga gttaacaaag tcattagcac
     1081 gctataataa tgttgaatac aaagaggtgc ttacgggctt taaatttatt gctcaggaaa
     1141 taagacaact tgatgatcat caaaatatga ttttcgcctt tgaagagagt tacggttttc
     1201 tatcagagcc ttttgtacgt gataaagatg ccgtacaaat tgtgccactc attataaaat
     1261 acgcttctga gttaaaatta tatggcaaaa cattgaaaga tgaattagaa cagatttatc
     1321 aaactgttgg tagacatgag gatacattat tctcgcatac gctagaagga ttagaaggta
     1381 agaaaaaaat tgaatcaatt atgacacatt tccgttcaca tccaccgcaa gagattcaag
     1441 gactgaaagt gaaagcaatt gaagattatt taactagcga ggttcatcag ttggataaag
     1501 atacaacgtc gcagattaat tctcctaaat caaatgttat tcgtgtctta tttgacgaag
     1561 gatttatcgc tttacgtcct tctggtactg aacctaaaat taaattatat gtgtcgttaa
     1621 aatgtccgaa ttttgatgat gtagctcaaa aaattaatgc gatgattttt agctaattaa
     1681 aaattttata catcgaaata ttacatttca ttttatactt atcagccctt ttgaaactag
     1741 aatccttatg ttttcgcgtt ttgaaggggc taatttgtaa taatttttca aaagtatatt
     1801 agaaaattaa ttttcatgta tttttcatat taagcggtaa taatataata attaataccg
     1861 cgacctattt aattgttttc gcgatgaaaa aggtttagaa ttacaaaaat aactatttta
     1921 aatttagtta caaatatatt ttagaaagga tgtgaaataa tgaaaggctc taaacaaata
     1981 cttttgatta tgggcattat atctcttatt gttttattta tttttacact attcatcatg
     2041 gcgcaatatg caaaacatta tgaacaaaaa tccgacagtt ccaacgcaca tactttaaat
     2101 tcatcatcag ccataattga gcaacatact atgtcgaatt tagcgtctct agatttatac
     2161 gctcctgttc gtaacattac ttctagtcgt gatattcatc ccatttattt catgacaaaa
     2221 gattaaaact tcctttttca taaccccatt cattcaacta ataaattaaa aaatgataaa
     2281 agcgtactgt tttttagaaa catacatata taagaaacac atccattact aaaagacagt
     2341 acattatatt ctatcgggaa ggtgcaacaa tgaccgtcaa catcgtagtc tctattgtgt
     2401 tttgtatcag tatggttgta ttaagcattt atgtcgcaat aactaaagat ttcacactaa
     2461 tttcttatat aaatcaaacg accattgcag ataaacacaa gaaccaaatt gcatatatat
     2521 ttgcgttatg tatcagtttg agtgcagtat ttttaatgtc tgcaattcta tgctttgaat
     2581 atgattttat tgcattggca ttcttatttt taacgatagc attattactt atcgcattat
     2641 tctacgtttg cttttataaa attacaaaat acccataaat ggttgcgtta aattggtagt
     2701 aatttttcgg tgcgctgtca gtaagcaatt gtttgcgaaa aaaaatacaa ccttgtacga
     2761 atacaaagtt gcattaatat agaatcattt atgcttttta atgttataaa cctaattctt
     2821 taataaatcg tgttaattct tctcgcatac tgtcatcttt caatgcatat tctatggtag
     2881 ttttaacaaa gcctaatttt tctccaacgt cataacgttc gccttcgaag tcatatgcat
     2941 acacttggtt atcattattc atacgctcaa tcgcatctgt taactgaatt tcgttacctg
     3001 cgccttcttt ttgcgttttt aaataatcga aaatttcagg cgttaataca tatcgcccca
     3061 taatagctag gtttgatggt gccgtacctt gtgctggctt ttcaacaaac tttttcactt
     3121 catactgacg tccattttta gttaatggat caataattcc ataacgatga gtatctgctt
     3181 ccggaacttc ttggacacct ataactgagt gccccgtttc ttcataaacg tcaatcaact
     3241 gtttcactgc tggcacttca gattcaacaa tatcgtcacc taataatacc gcaaatggtt
     3301 cattgccgat aaattgacgc gcagaactaa tcgcatgccc caaacctttt tgttctttct
     3361 gccttacata aaaaatattc gcaagttccg ttgaatactg aactttttct agtaattcag
     3421 atttaccttt ttcttttaaa accatttcta attctttttg actatcaaaa tgatcttcaa
     3481 tcgcgcgttt gtggcgacct gtcactataa taatatcttc aattccagct cttgcagctt
     3541 cttcaacgat atattgtatt gtgggtttat ctaagatagg aagcatttcc tttggcatcg
     3601 ctttagttgc tggtaaaaat ctagtcccta aaccagcagc gggaatgatt gcctttttta
     3661 tttttttcaa agttaatgtg ctccttttcc taagtattaa atctatgtat caacgtcatt
     3721 ttaacactaa ttagaacgcc ttcatagtgt cattgagtat gtaattattt cttgggaaat
     3781 ttgatttaat tttaaaaaac aggcttactt catataattt atgaaataaa cctgtcaatt
     3841 ttagattgat tatgctttgt gattcttttt atttctgcgt aataatgcta aacctagaat
     3901 actaaataat ccgccgaaca gtataccttt gtttgttgat tcttctccac ctgtttcagg
     3961 tagttcagat ttcttagatt gtttttgttt agttggtgcc actgctttaa ccttttcatt
     4021 gatttcaata acaggtgtta ctactttacc ttgttccact ggtttagaag gttttttagg
     4081 ttcttcttta gcaggtggta ctggtttacc aggttcagat ggtacctctg gtgttggtgg
     4141 tactggtgtt tccggctcac ttggtacttc cggtgttggt ggcgttggtt cgcctggctc
     4201 actcggtact tctggtgttg gtggtgttgg tgtttccggc tcacttggta cctctggtgt
     4261 tggcggcgtt gtatcttctt caatcgtttg ttgaccttca ttttgaccac ttacttttgg
     4321 aagtgtatct tcttcaaaat caacactgtt gtgtccacca aattgataat tag
//
LOCUS       DAPIDM010000172         4347 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000172 DAPIDM010000000
VERSION     DAPIDM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.174"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..192)
                     /locus_tag="RJE42_002459"
     CDS             complement(<1..192)
                     /locus_tag="RJE42_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDV5877850.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLY"
     gene            534..794
                     /locus_tag="RJE42_002460"
     CDS             534..794
                     /locus_tag="RJE42_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877851.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            822..1010
                     /locus_tag="RJE42_002461"
     CDS             822..1010
                     /locus_tag="RJE42_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877852.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            1198..1767
                     /locus_tag="RJE42_002462"
     CDS             1198..1767
                     /locus_tag="RJE42_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877853.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKTY"
     gene            1855..2139
                     /locus_tag="RJE42_002463"
     CDS             1855..2139
                     /locus_tag="RJE42_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877854.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKNAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            2200..2418
                     /locus_tag="RJE42_002464"
     CDS             2200..2418
                     /locus_tag="RJE42_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877855.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEIDLEN
                     GNISNIDYSLLVSVDLKALEISIFDAVN"
     gene            complement(2915..3115)
                     /locus_tag="RJE42_002465"
     CDS             complement(2915..3115)
                     /locus_tag="RJE42_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV5877856.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(3472..4158)
                     /locus_tag="RJE42_002466"
     CDS             complement(3472..4158)
                     /locus_tag="RJE42_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV5877857.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKEQYAKRFFFFATSCL
                     VLTLVVASSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT         1474 a          663 c          565 g         1645 t
ORIGIN      
        1 atataagcta atagctctat ttgtagtttc aattaattga tgatctgtgt aattagacat
       61 cacatatgct ctaattactt ggtgtctttc ttctaatcga gggaatgctt tatcttcact
      121 aacgtgttcg gccaatatac caaatggtgt gttatcattt tgaaaaccca tgatgaaatt
      181 gtaaaacgtc ataagacttg cccccttcat ttattaaacg ctatttaaag tgttattatt
      241 aatcttatca gaaaactagt atttgtaaat gtgcaaaagt aattttgcac atttttttgt
      301 tatctgattc taaaaatatt aacgaattat tcaactttaa gggtttttag catgtaaata
      361 ttcttttcat attagtacta tacaccccta tatatgctcg cttgtttgaa ttaataaact
      421 ttagcattct acaaataata ttttaaaaaa taactatact ttttatttac ctattggaaa
      481 atagtgttat catatttatt aagaagctat ttttatacta gtaggtgtca gtcatgaata
      541 acattttgtt aaatgctatc aatatagtta ttactaccac ttttgttatc tttaatattt
      601 taatcacata taataaagat ttagatgatt tatgttggct cctgcctggt attatcattt
      661 gtggtgtgat actcatagta tcctttacca ttgcaatgat aactaaaaac tggttaagtg
      721 aaatattatt ttttataaat atcgtactcg ttctctatta catttatcct attttttata
      781 gttttatagg ttaatattac ggtatacaag gagggtttca catgtcttta cattttgcaa
      841 ttctgttttg gctagcatta attttcttag ttgccgctac gtttatactc gtattaatga
      901 aaaaaactgg caaagaatct aaaaaagagt cctatttaag tttcactgtc attctctata
      961 tttttggatt cgctatatta atatacacat ttatatttgg tgtgctataa atgaaattca
     1021 ttttatatcc agtctgaaac gtctcaatga tgtttcagac tgttttttat tcttaataga
     1081 catttcacac tctcatactt taatatttaa gatatgtttt agtcttgcaa gcttattaaa
     1141 gattacaatt ctaatttaag actatttaca tatatttctt caacaaggag cttttaagtg
     1201 aactatatta aacgaacgat aattttactt atattattcg ttgtcgtatc ccctataaac
     1261 tcaccaaaaa caattgcaga taataagtat tcagaaattc aagatgacaa attccaatta
     1321 caacctggtg atattattgt tacaaaaggc cctgtcatgt ggggattttt tggtcattgt
     1381 agtatcgcga ttgatgataa aacgatttta caaattgagg ggcctggcga caaaccaact
     1441 acacaatcct tcgaatcttt taaatataat tatgcaagtg gcaaaaatga ttggatgaaa
     1501 gtttatcgtt gtagctatcc tggtgcaggt aaaaaagcgg ctgaatgggt taagaaaaac
     1561 tatgaaaata caaatcatcg ttatctagtt acattaaatt tgaacagcaa aaaatttaca
     1621 tattgtacta aaattatcta tcaagcttat aaatttggtg taagtgaaaa atcagttaaa
     1681 agttatggac tacatattat ttcaccctat gcaattaaag ataattttat agatccatat
     1741 aaactaaggc ttgttaaaac ttattaaaac tgtttcatca aaaagttaaa ttagtttgtg
     1801 ttttgcaata aattgggtat agattacaaa tgatatttca ggaggcccaa tattttgaat
     1861 gaactaacta aagaacaaaa gtatacaata gctaaatttt acaaattata tattgaacgt
     1921 tcgaataatg gagaaacaga aacagtcgct aacttttttg gcgatgctaa aaatgcacgt
     1981 gaaaactatt tttgcgatcg tgattatcaa gacttcttaa ctaactgcca aattttaatt
     2041 caaaacaaat acttaactgg tgaagttttg gacgataaca tttacaatat ttctatttta
     2101 aacaagacat ttattgaatt tgaacaaaat tttggttgaa catggatgtc tcaactataa
     2161 aatttctaac cctattttaa aacaggagtg aaaatataca tgagtgaatg gcatattaat
     2221 aatcaatcta ctaaaccttt tttgatacaa caagcggaaa agcaattgtc tattgttaaa
     2281 ccgttgccta atggtagctt ccaaatactc acagaaatag atttggagaa tggcaacatt
     2341 agtaacatcg attattctct acttgtttct gtagacctca aagcattaga aataagtata
     2401 tttgacgctg taaattaaac gcaatatttt tatttaatta ttttaatgtt ccacttttta
     2461 aatgaaaaca agattactga attattatga gattttaaaa acaataaata ttctcaataa
     2521 ttaatcagtc ttaataaaaa attaacaaaa agttgattag ttaaattatt aattaagtag
     2581 taagatatta tgcgatgtgg tatcctatta ttgttaaaca ttttgagtgt ttaacattac
     2641 ttcttgttat cgccatatac agactttaat tctcgtcatt attaaagttc tgtattggcg
     2701 ctttttttat attcaaaatc aaaagtcgga cagatgaaaa gtaaaaatct tttccatcag
     2761 ttcgatttat tttagaacca ttatatattg ttagatctcc acctaaattt attggacaaa
     2821 aaaataacag ctagtgcttt taccacacca actgctatcg atttaaagca taaaaaaaca
     2881 gccctataat aaggactgtt tgaataagtt aaaattacat tttaactacg tttgcagctt
     2941 gctcgccacg gtcgccttca acgatgtcga attcaacttt ttggccttct tctaatgatt
     3001 tgtatccatc ttcagcgatt gctgagaagt gtacgaatac gtcgctacca tcttctcttt
     3061 cgatgaaacc aaaacctttt tctgcattaa accatttaac tgtaccgtta ttcatattga
     3121 atacctccgt cgtgcttttg cactaaatat tttgtaacaa attcataaat gaaaaggaga
     3181 atattctata taaataactc acatttttct tcacgctctt tattacttaa ctttcattat
     3241 acacttttaa atatagaagt aaagcattat tttattttct gtgataatac acattgaaat
     3301 atatagtaat attccatttt aatactaaat ttaaaatttt tattttctaa tattaaatac
     3361 acttgatttt gatactattt acttttttaa ttctgaattg ataataaatc ataacgtgat
     3421 ctaaaaattg taaaagtgcc actagcagca gtgacacttt tacaatgagc attattgacc
     3481 tgaatcagcg ttgtcttcgc tccaaatatt taatttctct tttttcgctt gtgcttcact
     3541 ttttcttaaa agttgttcat gtgtattgtt aggtttataa acataagcaa ctttagccaa
     3601 gccttgacga actaaagctt cgtttaccat ttttccatca gcataaatat acgctaagcc
     3661 acgtccatat ttatcagttc tttgaccttt gtcaaactcg acttcaattt tctttgcatt
     3721 ttctaccatt tttttcgtaa atgcacttgc ttcaggacca tatttctcta cacctttttt
     3781 aggatgcttt gtttcaggtg tatcaaccaa taatagtctg aatgtcattg gttgaccttt
     3841 gtacattaat ttaacagtat caccatcaat cgctttaatt aatgtcgcag gttctttatg
     3901 taatttttta gttgaagttg cactatatac tgttggatct tctgaaccac ttctatttac
     3961 gccattatct gtttgtgatg catttgctga gctacttaga cttgaagcta caactaaagt
     4021 taacactaag caactagtag cgaaaaagaa aaacctcttt gcgtattgtt ctttcgaaac
     4081 attactgata gccatcccta taagtaatat tgaaacaatt gccatacata tgccagcact
     4141 taataagtat tctgtcataa ctaacacctc tttcttttta gttaatttta atattaaacg
     4201 ttaattaatt ataattctac atttttacaa ttaattaata aaaatttaat aattataata
     4261 aaataactat acttactttt atgtattttc tatattttag gtgtcagaac ttaaatattt
     4321 agtcgtattt gtaaaattaa aaagcct
//
LOCUS       DAPIDM010000173         4257 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000173 DAPIDM010000000
VERSION     DAPIDM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.175"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..156)
                     /locus_tag="RJE42_002467"
     CDS             complement(<1..156)
                     /locus_tag="RJE42_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877858.1"
                     /translation="MLYTKPLIGDRTPAKFAPSQMDESDAFTESVKKKYPDSEIHPIG
                     HSLGAFLA"
     gene            complement(226..531)
                     /locus_tag="RJE42_002468"
     CDS             complement(226..531)
                     /locus_tag="RJE42_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877859.1"
                     /translation="MKYTDKIYHKVSDFSYSKLKKDNTYKILGQDYKVIEKRDDTQNG
                     LRAYVFAPVIKGKVDTSQLLIGYAGTDPFSLNDIRTDLQLPIHHNTNNLKINNHYKN"
     gene            574..1017
                     /locus_tag="RJE42_002469"
     CDS             574..1017
                     /locus_tag="RJE42_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877860.1"
                     /translation="MERKKIIFIISLIIALLISTGYLINKNKKDHYIEIQEKRIDLYF
                     KYNLNNYHSMKVTSFKKTPMGGYIVDGYVNHNKNYDFKVLISATDNHQFEDSIGYDDK
                     TFGKLFKEKDHKNELKSTDIIKKEHLDKSEYEAEPPLFFFSGPIE"
     gene            1226..1603
                     /locus_tag="RJE42_002470"
     CDS             1226..1603
                     /locus_tag="RJE42_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5877861.1"
                     /translation="MNKKFLMIVFIIIILILVGIFLKIKYDEKKYYDKQKERITIYMK
                     HNVKGYKNISFTNFKENSMDGYSISGYINNDKKLSFTAGIRSIDDFQFDTDISYTDEL
                     GRKLIKNPKSVSEIKKEQNTSNK"
     gene            complement(1729..4152)
                     /locus_tag="RJE42_002471"
     CDS             complement(1729..4152)
                     /locus_tag="RJE42_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDV5877862.1"
                     /translation="MTYKMKKWQKLSTITLLMAGVITLNNGEFRNVDKHQIAVADTNV
                     QTPDYEKLKKTWLDVNYGYDQYDENNQDMKKKFDAKEKEAKKLLEDMKTDTNRTYLWD
                     SAKDLDKKSADMTKTYRNIEKIAEAMRHKNTSLKTDENKLKITDAIKWLHHNVYGKDP
                     DKKVTDLTTNRKEKDSSKKNNSLNWWDYEIGTPRALTNTLLLMDDMLTKDEMKNYSKP
                     ISTYAPSSDKILSSVGESEDAKGGNLVDISKVKLLESVIEEDETMMKNSIDSFNKVFT
                     YVQDSATGKARNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKESPFKHTQDK
                     ATLSNWIDEGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDTMDDST
                     KAKYKQIVKTSVNSDSSYNQNDYLNSYSDIDKMKKLMDDSTISKNDLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINNENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMTCLPGTTTLNDMPSTNTKNDKSFVGGTKLNNKYASIGMDFENQDKTLTAKKSY
                     FILNDKIVFIGTGIKSTDSSKNPVTSVENRKAVGYKLFKGDIEITTSDVNAQETHSVF
                     LESNDTKKNIGYHFLDKPKITVKKESHTGKWSEINKSQKTDDKKDEYYEVTQTHNTSD
                     SKYAYVLYPGLSKSDFKSKNNNVSIVKQDEDFHVIKDNDGVFAGVNYSDSTKSFDING
                     TIVELKEKGMFVIKKKDNNTYECSFYNPTSTNSTSNKESKISVTGYTITNQSVSNFKE
                     SDIHFELTK"
BASE COUNT         1392 a          627 c          612 g         1626 t
ORIGIN      
        1 agctaaaaat gcacctaaac tatgtcctat tgggtgtatc tcactatctg gatatttctt
       61 ttttactgat tcagtaaaag catcactttc gtccatttga gacggtgcaa atttagcagg
      121 tgttcgatca ccgataaggg gtttagtata taacacagaa gaaaaactaa aaaggtcatt
      181 tttatctttt attgtattgt attttttgtg aatagttgag tttttttaat ttttataatg
      241 attattaatt tttagattat ttgtgttatg atgaatggga agttgtaaat cagtacgaat
      301 atcattaaga ctaaatgggt cagtgccagc ataacctatt aaaagttgac tggtatcaac
      361 ttttcctttg attactggag cgaaaacata tgctctcaat ccattttggg tgtcgtcgcg
      421 tttttcgatg actttgtaat cttggcctag aattttataa gtgttatctt tttttaactt
      481 tgaataagaa aagtcagata ctttatgata gattttgtct gtatatttca taagattacc
      541 tctttaacat taaatttagt gaggatgaaa aaaatggaaa gaaaaaaaat aatatttata
      601 atttccttaa ttatagcact cttaataagt actggctatt taatcaataa aaacaaaaaa
      661 gaccattaca ttgaaataca agaaaagaga atagatttat attttaaata taaccttaat
      721 aattatcatt caatgaaagt tactagtttt aaaaagacgc ctatgggtgg ctatattgtg
      781 gatggatatg ttaatcacaa caaaaattat gattttaaag ttctcatctc tgcaactgac
      841 aatcatcaat ttgaagatag cataggatac gatgataaga catttggtaa attatttaaa
      901 gaaaaagacc ataaaaatga attaaaatct acagatatta taaaaaaaga acacttggat
      961 aaaagtgaat atgaagccga gccaccgtta tttttctttt caggtccaat tgaataaatg
     1021 ttatgaatag aatggataaa aatataatta tttaaatttt aaagtaaatc aaatcatgat
     1081 ttaagattat aagaaataaa aatttactaa tgttaaaacc aacagtcatt tgggttgcta
     1141 cgattttaca atcatcgtta aactcagtat gcttcattaa tttaaaatgt tcgtcaataa
     1201 atccaatgaa aaggagattg attttttgaa taaaaaattt cttatgattg tatttataat
     1261 tataatattg attctagtcg gaatcttctt gaagataaaa tatgacgaaa agaaatatta
     1321 tgataagcaa aaagaaagaa taacgattta tatgaagcac aatgtgaaag gttataaaaa
     1381 tataagcttc actaatttta aagaaaactc aatggatggt tattctatta gtggttatat
     1441 aaataatgat aaaaagttat catttacagc tggtataaga tctattgatg attttcaatt
     1501 tgataccgat atttcttata cagatgaatt gggtagaaaa cttattaaaa atcctaagtc
     1561 agtttctgaa ataaagaaag agcaaaatac gtccaataaa taattgttca tactgtggtg
     1621 aaaccaaaat atataaatca atagatgacc ttaactatga tataaaattt tgcaatattt
     1681 ataaaacctc gacactataa aaatgttata gtgccgaggt tttattgctt atttagttaa
     1741 ttcaaagtgt atgtcagatt ctttaaaatt tgagacactt tgattagtta tagtgtaacc
     1801 tgtaactgaa atttttgatt ctttatttga agtggaattt gtggatgtcg gattataaaa
     1861 actacattca tatgtgttat tatctttctt tttaattaca aacatacctt tttcttttaa
     1921 ttcaacaata gttccgttta tatcaaaaga tttagtacta tcactataat taaccccagc
     1981 aaatacgcca tcattatctt ttatcacatg aaaatcttca tcttgtttaa caatacttac
     2041 attattattc ttcgatttaa aatcactttt tgataaacca ggatacaaaa catatgcata
     2101 tttactgtca gatgtattat gtgtttgagt tacttcataa tactcatctt ttttgtcatc
     2161 tgttttttga cttttattaa tttcactcca cttaccagta tgactttctt tttttacagt
     2221 tatttttggc ttgtctaaga aatgataacc aatgttcttt tttgtatcgt tggactctaa
     2281 aaagactgaa tgggtttcct gagcattaac atctgaagtg gtaatttcaa tatcaccttt
     2341 aaataattta tacccaactg ctttgcgatt ttcaacactt gtaactggat tctttgatga
     2401 atcagtactt ttaatgccag ttcctatgaa gacaatttta tcgtttaata tgaaatatga
     2461 ttttttggca gttaaagttt tgtcctgatt ttcaaaatcc ataccgatgc ttgcgtattt
     2521 attatttaat tttgtcccgc caacaaagga tttatcattc ttagtattag tagatggcat
     2581 atcatttaaa gtagtagtgc ctggaagaca agtcatatcg gctgttgccc aaaagttatc
     2641 acgatagtgt ttgacatcgc tgttatataa ataagacatg cctgcaccgg tgtgccaacc
     2701 ttttaagttt tcattattga tactttcgta acgtgcgacg tttttcgatg tcatacttaa
     2761 tccaaatgca aagtcaaggt ctttattgtg ataggtgaca cgatccatgt cattatatat
     2821 tttaagttgt tgtgttaaat catttttaga aatagtgcta tcatccatta actttttcat
     2881 tttatctatg tctgaatatg aatttaaata atcattttgg ttgtaacttg aatcagaatt
     2941 aacagaagtt ttgacgattt gcttatattt agcttttgtt gaatcatcca tggtatcact
     3001 caatctcaac aatgatttca ttacagttgc tgacgctgta tgactcgttt cattttcacg
     3061 actgatagct ctacctcttg ataaatccat catttcacct ttatagatta atggcataaa
     3121 accttcgtca atccaattgc ttaatgtagc tttatcttgt gtatgtttaa aaggagattc
     3181 ttttatcatc ggcatcattt gagaaatacc ctctaatagt acaacaccat aagcgccagt
     3241 gtaagggaca tcttgatgat caatgtaaga gccatcttta tagaatccat tgcgagcttt
     3301 accagtggca gaatcttgaa cataagtgaa cactttatta aatgaatcta ttgaattttt
     3361 catcatagtt tcatcttctt caataacact ttctaaaagt tttactttag aaatgtctac
     3421 taaatttcca cctttagcat cttctgattc accaacagaa gataaaattt tgtcactgga
     3481 tggtgcatat gtactaatag gttttgaata atttttcatt tcatctttag tgagcatatc
     3541 gtccattaga agtagtgtgt ttgttaatgc tcttggcgta ccaatttcat aatcccacca
     3601 attcaatgag ttattctttt tactagaatc tttttcttta cgattggtgg ttaaatctgt
     3661 gactttctta tctggatcct taccatatac gttatgatgt aaccacttaa ttgcatctgt
     3721 tattttcaac ttgttttcat ctgtttttaa tgaagtattt ttatgtcgca ttgcttctgc
     3781 gattttctcg atattacgat aggtttttgt catgtctgcg gactttttat ccaaatcttt
     3841 tgcactatcc cacaaatatg ttctatttgt atcagttttc atatcttcaa gtaacttttt
     3901 agcttctttt tctttagcat caaacttctt cttcatatct tgattattct catcatactg
     3961 atcataaccg tagttaacgt cgagccacgt cttcttcaat ttttcataat ctggcgtttg
     4021 aacattcgta tcagccacag caatttgatg tttatcaaca tttctaaatt caccattatt
     4081 taaagtaatc actccggcca ttaataacgt aatggtggat aatttttgcc atttcttcat
     4141 tttatatgtc attgacatgt ctcctttttt tgcgtgtatg caattgatat tatgattaat
     4201 taatgattca actttccaaa ggattgggaa attcgttgat gcatggaaac catcagt
//
LOCUS       DAPIDM010000174         4256 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000174 DAPIDM010000000
VERSION     DAPIDM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.176"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(25..624)
                     /locus_tag="RJE42_002472"
     CDS             complement(25..624)
                     /locus_tag="RJE42_002472"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV5877863.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(971..1225)
                     /locus_tag="RJE42_002473"
     CDS             complement(971..1225)
                     /locus_tag="RJE42_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV5877864.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(1343..2659)
                     /gene="rho"
                     /locus_tag="RJE42_002474"
     CDS             complement(1343..2659)
                     /gene="rho"
                     /locus_tag="RJE42_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV5877865.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKKSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(2906..>4256)
                     /locus_tag="RJE42_002475"
     CDS             complement(2906..>4256)
                     /locus_tag="RJE42_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDV5877866.1"
                     /translation="PATEEVIGKVAKGNKADVDKAVEAADNVYLEFRHTSVKERQALL
                     DKIVKEYENRKDDIVQAITDELGAPLSLSERVHYQMGLNHFVAARDALDNYEFEERRG
                     DDLVVKEAIGVSGLITPWNFPTNQTSLKLAAAFAAGSPVVLKPSEETPFAAVILAEIF
                     DKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRMMSFTGSGPTGSKIMEKAAKDFKKVSL
                     ELGGKSPYIVLDDVDIKEVAKATTGKVVNNTGQVCTAGTRVLVPNKIKDAFLAELKEQ
                     FSQVRVGNPREDGTQVGPIISKKQFDQVQNYINKGIEEGAELFYGGPGKPEGLEKGYF
                     ARPTIFINVDNQMTIAQEEIFGPVMSVITYNDLDEAIQIANDTKYGLAGYVIGKDKET
                     LHKVARSIEAGTVEINEAGRKPDLPFGGYKQSGLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT         1255 a          851 c          629 g         1521 t
ORIGIN      
        1 tacaatatct aattgaccaa acacttataa ttcctcctta ttattatcac taggtgctac
       61 gatatggtgc gcgcgacaac gtggctcata actttcattg gcacctacta agataatcgg
      121 atcatcgatt ttagctggtt taccatttat taatcgttgc gttctactag atgaagaacc
      181 acaaacagca caaactgctt gaagtttcgt tacttgttca ctgacagcca tcaatttagg
      241 cattggttcg aacggttcgc ccctaaaatc catatctaat ccagcaacaa taacacggtg
      301 tccatctgct gatagttttt ctactatact tacaatttca tcgtcaaaaa attgcacttc
      361 gtctattcct ataacatcaa cattagttaa gtcgtgcgtc ataatttcac ttgctttaga
      421 aatattaatc gcttcaatgg cattaccatt atgagagacc actttttctt tatgatatcg
      481 atcatcaatc gccggtttaa atacaacgac tttttgttta gcgtatatac cccttcttaa
      541 acgtcttata agttcttcgg atttaccgct aaacatactt cctgtaatac attctatcca
      601 accggaatgg taagtttcat acattgagag ttccaccttt ttcaaaacat aatcgcttta
      661 ttatatcata tttcaaatat tcataaatgt ctttttcata attatatcga tattgtacat
      721 gaacaattat tttataaatt tgtcatgcat atttatatac agctattcta atttggtgct
      781 tcttattcgt gcgatgtttc tactcaatat aaactttaat agagggggaa tttatcatta
      841 tgtattaaca acatggtatt gagtcacttc tttgaacaca gtcgtaagta aataccctta
      901 ctacgctaat aaataaaaaa agctgtctac aattccatta taggatgcag acagcttaac
      961 gattactata ttagttgttt gatttaagac caaacttttt gttgaaacgt tccacacgtc
     1021 catccgcagc agcgaatttt tgacgtccag tgtagaatgg atgtgaatca gatgaaatat
     1081 ctaaacgaat aactgggtat tcttttccat cttcccactc catcatttct gaagatgttt
     1141 ttgtagaacc gcttaagaat ttgaagtttg tagtagtatc taaaaagatt acttggtgat
     1201 actcaggatg aattccttgt ttcattattt tcagctcctt tgccctgaac catctggaac
     1261 agagttattt gtgagttttt acccaatact gtgtaattat aaaggtaatt aattcaaaac
     1321 gcaagcccct atataatgtt tattaaatta taggtcgacc tgttttagta ctttcttctg
     1381 cagacttttg taactgttta aagaagtctt cgttattctt agatttttta agtttacgaa
     1441 taaatctttc agtgaagtcc gttgaatctg taaatagatt tctcaattgc cataatgtat
     1501 ctaattccga tttactaatc aacaattctt ctttacgtgt tgagcttctg ccaatatcaa
     1561 tagcagggaa aatacggcgt tcagataatt tgcgatctaa atgtaattcc atatttcctg
     1621 tccctttaaa ctcttcgtaa atcatgtcgt ccatacgtga acccgtatca actaatgccg
     1681 ttgctagaat agttaaactt ccacccgctt caatgtttct cgcagcacca aagaatgctt
     1741 ttggtttgtg taacgaagct ggatccaagc cacctgacaa cgtacgacca cttggtggaa
     1801 taaccaagtt ataagcgcgt gctaatcttg ttatagagtc cattaaaata atgacatctt
     1861 caccaatttc tactaagcgt tttgcacgtt caagtaataa ttcagctact ttaacatgat
     1921 gttctggtgg ttcgtcaaat gtagaatgca caacttcagc agcctctact gaacgttcta
     1981 aatccgttac ctcttcagga cgttcgccaa ctagtaaaat aaataatttt gcatctggtt
     2041 tgttcgtact gattgcattc gctatttctt ttaataacga tgttttacct gctttaggtg
     2101 gcgccactat taaaccacgt tgacctaaac caatcggtgt tactaaatcc atgatgcgcg
     2161 ttgaataatt ttgtgtttct gtctctaatt taatacgctc atctggataa agtggtgtca
     2221 aagcttggaa atgcggtcgt ttcttcactt cttctgcgtt atggtcattg acaaagtcaa
     2281 cttgtaataa gccataatat ttttcgttat ctttaggttt tctaactttc ccagttactt
     2341 tatccccacg tttaatttca aaacgacgaa tttggctagc agatatataa atatcttttt
     2401 cccctttaga atagttcact gttcttaaaa aaccataacc atctggttgt atatcatcta
     2461 agataccttc catataatag ttaccatctt tttccatttg tgcttccata atagctagaa
     2521 caagttcttt tttatttaat ttactatagt tcgtcaactt aagagtttta gctttttgag
     2581 tgagttcctt ggtagtatag ttcttgtaca attcgtggaa tgattcatac tgaggagatg
     2641 tacgttctct ttcaggcata tagtttacac ccatttcatt attttttagt tagtaagtaa
     2701 tagataggca tgacaatgca taaataactg tgctagcaca atcccattat gctaatcccc
     2761 aaccttttaa atacatgaat aactatagca aatttttatt ttaattacaa aatctattta
     2821 taaaaattgc aaaattaaaa cagtacacaa aacttaatta gtcatgtgca ctgttttatt
     2881 gcgattttta attcaaaaat caaatttatt taaaatagcc agctatagat ttcacttcta
     2941 agaactcttc aataccataa tcgccccatt cacgacctaa accagattgt ttatagccac
     3001 caaatggtaa gtctggcttt ctacctgctt cgtttatttc tactgtacct gcttcaatag
     3061 aacgagctac tttatgcaat gtttctttgt ccttaccaat aacatatcct gccaaaccat
     3121 attttgtatc atttgcaatt tgaattgctt catctaaatc gttataagtg ataactgaca
     3181 ttactggccc aaaaatttct tcttgtgcta tcgtcatttg attatctaca ttaataaaaa
     3241 ttgtcggacg tgcaaagtat cctttttcaa gtccttctgg tttaccagga ccaccataaa
     3301 ataattcagc accctcttca atacctttat taatataatt ttgtacttga tcaaattgtt
     3361 ttttactaat gatagggcct acttgtgtac catcttctct tggattcccg acacgcactt
     3421 ggctaaattg ttcttttaat tcagctaaga atgcatcttt aattttgtta ggcactaaaa
     3481 cacgtgtacc agctgtacat acttgaccag tattattaac aactttgcct gttgttgctt
     3541 tagccacttc tttaatatct acgtcatcta gaacgatgta tggtgatttg ccaccaagct
     3601 ctaatgatac ctttttaaaa tctttagcgg ctttttccat aattttagaa ccagtagggc
     3661 ctgatcctgt aaatgacatc atgcgtactt taggatgttc agataaagga tttccaacgc
     3721 cagcaccatc accattaaca aggttaaata cacctttagg aacaccgact ttatcaaaaa
     3781 tctcagctaa aataacagct gcaaatggtg tttcttcaga tggtttaagt acaactggac
     3841 taccagccgc aaatgctgct gctaatttta atgatgtttg gtttgtaggg aagttccacg
     3901 gtgtaattaa gccagataca ccgattgctt ctttaacaac taaatcatct ccgcggcgtt
     3961 cttcaaattc gtagttatct aatgcgtctc tcgctgcaac aaaatggttt agtcccattt
     4021 gataatggac acgctcagat aatgataaag gagcacctaa ctcatccgta atagcttgta
     4081 caatatcgtc ttttctgttt tcatattctt ttacaatttt atctaataat gcttgtcttt
     4141 ctttcacaga tgtatgacgg aactctaaat aaacattgtc tgccgcctcg acggctttat
     4201 caacatcagc tttattacct ttagcaactt tcccgattac ttcttcggtt gctgga
//
LOCUS       DAPIDM010000175         4238 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000175 DAPIDM010000000
VERSION     DAPIDM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.177"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            55..276
                     /locus_tag="RJE42_002476"
     CDS             55..276
                     /locus_tag="RJE42_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877867.1"
                     /translation="MEFKKQFPDYLNYKNEEYLMNVVTEELLREIAQRLPRVYIQQKQ
                     QNRYLNLDETIKNACDVVSIYTNNKKSGI"
     gene            344..799
                     /locus_tag="RJE42_002477"
     CDS             344..799
                     /locus_tag="RJE42_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877868.1"
                     /translation="MDATSELAKNDNVFEDYNFGYKLTNESKYNWLCINPNVGEVVNF
                     EELIITTSELCKQTAEHIKQAQEQLNRVNELRARKDAIRDCLSAMEALMTYLTGTTDI
                     SAADKAMRDDEETWGQGFIIKEGITMWNMFHNKYKDIRHGNNFDISLIS"
     gene            944..2782
                     /locus_tag="RJE42_002478"
     CDS             944..2782
                     /locus_tag="RJE42_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDV5877869.1"
                     /translation="MLPFFTNPYPDELLYSAIARYHFYSGNLDCKDTLEELFDSRSVI
                     PSMEIGSHLTNLVEKLGPQYSVEYLLANHTIYPYYASFLSKQRQMEILEDVKNNGQAL
                     YTRLGIVAGGICRKDGLYYCDMCAKSDSDKYREPYIHREHQLQGISYCAHHEIPLREY
                     PVTGDSRIEYIRFELRYMNLSDLYEVEPYEEIVIQLAKQAYKLLKIPLHSLTKVEIDS
                     MYKALLRKKNLITAANRVRQQDLYQMIKIDLSEEHLTYYESNFNEENEYNWVKVLTRN
                     STRHVNPLRHLLILLYLKQDVGDLIVDNGPFRKGPYPCLNRAAKHYKKLVIPSVKVTR
                     DFKTKAPIGTFCCSCGFVYARKGPDNNVEDQYRIGRIKAFGHVWRKLLKKLLNTGMSI
                     RAIAKELGVDPKTVKKYAEEPTLKEAKAIKPDRLETYRNDLSEIMCTQPQLTRTEIRN
                     QLCKQYMYLYRYAKEWLMANLPIQRKYTEPNRLVDWNARDREYVQRIKALHQELLTVE
                     KPVRITNSLIWKRLGITANIEGYSAKLPSTTTLLNEIIETVKAFQIRRCCKVIDQMLE
                     ENEVIKLWKVQRIAAIKSHHFHEIKKELSKYIQKSQGVKRDEPQSI"
     gene            2872..3093
                     /locus_tag="RJE42_002479"
     CDS             2872..3093
                     /locus_tag="RJE42_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877870.1"
                     /translation="MHILERKGELEKILIDWGMLPILAIQHMYIDGDLRHSLPPDNLE
                     EVDLTIFPQLVRYTERMDCTRNERSGYVK"
     gene            3385..3705
                     /locus_tag="RJE42_002480"
     CDS             3385..3705
                     /locus_tag="RJE42_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877871.1"
                     /translation="MKFEWLFKQPISIKAVVKTGGIILRVNEVKKKEIPFDVISFSHP
                     ELSKYEKSNEPKKYTMHELKLSTEEKEKIIDETVEGATEDFELAEMNKQVHEYAESPS
                     IVKI"
     gene            3883..>4238
                     /locus_tag="RJE42_002481"
     CDS             3883..>4238
                     /locus_tag="RJE42_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877872.1"
                     /translation="MQELEDYKEVQLIIIQMSSLPIGDGKRVFSYLEDGVTPRQYALA
                     TVSLFNGNEFKILEVERENCALSMLILSSTGLVNWNPLIDSLLLNLVNSSGTWVKESL
                     EILERSNVIIQKAKHS"
BASE COUNT         1602 a          530 c          837 g         1269 t
ORIGIN      
        1 aaaaatccaa tggtagaatt tttcgtaact ttggaatgag gagtagaatt tttgatggaa
       61 tttaaaaaac aatttcctga ttatttaaat tataaaaatg aagagtattt aatgaatgtt
      121 gtaacagagg aattacttag agaaattgct caaagactac caagggtata tattcagcaa
      181 aaacagcaaa acaggtattt aaatttagat gaaactatta aaaatgcttg cgatgtagta
      241 tcgatttaca ctaataataa aaaatcgggg atatgatttt attattagag attttgaaga
      301 gcaagttgta gattttaaaa aaataggtaa attccataaa tttatggatg ccacgagtga
      361 attggcgaag aatgataatg ttttcgaaga ttataatttt ggttacaaat taacaaatga
      421 atctaaatat aactggttat gtattaatcc taatgttggt gaagtagtga attttgaaga
      481 attgataatt actacttcag agctttgtaa acaaacagca gaacatatta aacaagcaca
      541 ggaacaactg aatagagtga atgaacttag agctagaaaa gatgctatac gtgattgttt
      601 atcggctatg gaagctttaa tgacttattt gacaggaacg acggatattt cagcagctga
      661 taaagctatg agagatgatg aagagacatg gggacaaggt tttattatta aagagggcat
      721 tactatgtgg aacatgtttc ataataaata taaagatatt aggcatggga acaactttga
      781 tatcagttta attagttagg aagaaactat ttattttatc gacaaacttc ttgtttatgt
      841 aaagtatctt tctagtaaag caccaagtac tgatattatt aagaaaagta aagaagagtt
      901 ttagtttaat tagaaatttt tattgaatat aagaggtaat taaatgctac ctttcttcac
      961 gaatccatat ccagatgaat tattgtattc agcaattgca cgttatcatt tttatagtgg
     1021 gaatcttgat tgtaaagata cattagaaga gttatttgat agtcgttctg tgattccgag
     1081 tatggagatt ggtagtcatc ttactaattt agtagagaaa ttaggacctc aatattcggt
     1141 agagtattta ttagctaacc atacgattta tccttactat gcttcatttt tatctaaaca
     1201 acgacagatg gagattttag aagatgtcaa aaataatggg caggctttat atacaaggct
     1261 tggtattgta gctggaggca tttgtcgtaa agatgggcta tattactgtg atatgtgtgc
     1321 taaatctgat tcagataagt atagagagcc gtatattcac agagaacatc aattacaagg
     1381 aataagttat tgtgcacacc atgaaattcc gttgagggag tatccggtaa caggcgatag
     1441 tcgcatcgaa tacattcgct ttgaattacg ctacatgaat ctatcagatt tatacgaagt
     1501 ggagccatat gaagaaatag tcattcaatt agcaaagcag gcatataaac tattaaaaat
     1561 accgttacat tcattaacca aagtagaaat agattcgatg tataaggcat tacttagaaa
     1621 gaaaaattta attacagccg ccaatcgggt gagacaacaa gatttatatc aaatgataaa
     1681 aatcgactta tcagaagagc atttaacgta ctatgagagc aattttaatg aagaaaatga
     1741 atataattgg gtaaaagtat taactcgtaa ttcaacgagg catgtgaacc cattacgcca
     1801 tttattaata ttgttatatt tgaagcaaga tgtaggagat ttgattgtag ataacggtcc
     1861 atttagaaaa ggtccttacc cgtgtttgaa tagagctgcg aaacattaca aaaagttagt
     1921 tataccatct gtaaaagtta cgagggattt taaaacaaaa gctccaatcg gtacattttg
     1981 ttgttcttgt ggctttgtgt atgcgcgtaa agggccagat aataatgtgg aagatcaata
     2041 tagaattggt agaataaaag cttttggtca tgtatggcgt aaactactaa aaaaattgtt
     2101 gaatacagga atgagtattc gagctattgc taaagagcta ggggtagatc ctaaaactgt
     2161 taagaaatat gcagaagaac caactctaaa agaggctaag gcaattaaac cagatagatt
     2221 agaaacctat cgtaatgatt tgtccgaaat aatgtgtact caaccgcaat taactcgaac
     2281 tgaaataagg aatcaactat gtaaacagta tatgtatttg tatagatatg ctaaagagtg
     2341 gctaatggct aacttaccaa tccagcgaaa gtatacagaa ccgaatagac ttgtggattg
     2401 gaatgcgcga gaccgagaat atgttcaacg tataaaggcg ttacatcagg aattattaac
     2461 tgttgagaag cctgttcgaa taacaaattc tttaatttgg aagcgattag gtattacagc
     2521 aaatatagaa ggttattcag ctaagttgcc aagtacgaca actttattga acgaaataat
     2581 agaaaccgtt aaagcatttc aaattagacg ttgttgtaag gtgattgacc agatgctaga
     2641 agaaaatgaa gtgataaagc tttggaaagt acaacggata gcagcgataa agtcgcatca
     2701 ctttcatgaa ataaaaaagg aattatctaa atacattcaa aaaagccagg gtgtgaaaag
     2761 agatgaacca caaagtatat aaattgaaaa actggccatt tgctaaagga gaacaagctc
     2821 aattaacttg gattagttct ccatttaggc gtgagaagaa aatcatgatt tatgcatatt
     2881 ttagagcgaa agggagaact ggaaaaaatc ttaatagatt ggggcatgtt acctatatta
     2941 gctattcaac atatgtatat agatggtgat ttacgtcata gtttgccacc tgacaattta
     3001 gaagaagttg atttgacgat tttcccgcaa ttagtacgtt atacagagag aatggactgt
     3061 acaaggaacg aacgatcagg atatgtcaag tagttttata gtgaagttta ataataaatc
     3121 gtatacattg ccgctaattg aagtagttcg tagtatctta acgccctatc gctttttatt
     3181 atatcgctta tttgaaatga actcttttcc acagtatttt attgaaaact atacagaagg
     3241 acaaattcaa ttgagctttt cttctcaata caatctgaaa tacacgaaaa aagcttttta
     3301 tatcagctag tctggctatt aggtaatcag gatctaagaa aaacgtttga aaatattgca
     3361 tatacatttt taaatacggg tgaattgaag tttgagtggt tatttaaaca acctatttca
     3421 attaaagcag ttgtaaaaac cggagggata attttaagag taaatgaagt gaagaagaag
     3481 gaaataccat ttgacgttat atcattttca catcccgaat tatcaaaata tgaaaaatct
     3541 aacgaaccta agaaatatac gatgcatgaa ttaaaattat ctactgaaga aaaggaaaag
     3601 ataatagatg aaacggtaga aggtgcaaca gaggattttg aattagctga aatgaataaa
     3661 caagttcatg agtatgccga atctccaagc atagtgaaaa tttaaggtaa agcaataaaa
     3721 caaagaacat gtgaagatga aagtacgaaa agatatacat ttaaagatac aggaattcgt
     3781 tcagcatcag atacagcggg taatcgttta gcgagaggga ttgaaaataa agctttacag
     3841 aaagttcagg cagaaggcaa attaggtgag tttgtacatg tattgcaaga actagaggat
     3901 tataaagaag tacaattaat tattatacag atgagttcat tgccaatagg agatgggaaa
     3961 agagtattta gctatttaga agatggtgta acacctcgtc aatatgcact agcgacagtt
     4021 agtctattta acggcaatga gtttaaaatt ttagaagtag aaagagaaaa ttgtgcactt
     4081 tccatgttga ttttatcttc gacgggattg gttaattgga atccattgat tgatagcttg
     4141 ctgttgaatt tagttaatag tagtgggaca tgggtgaaag aatcgcttga aattttagag
     4201 agaagtaatg taatcataca aaaagcaaag catagtaa
//
LOCUS       DAPIDM010000176         4179 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000176 DAPIDM010000000
VERSION     DAPIDM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.178"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..248
                     /locus_tag="RJE42_002482"
     CDS             <1..248
                     /locus_tag="RJE42_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5877873.1"
                     /translation="THSVVQRAIKTLNKHAYFIKNTFDYYNLSNGPLEGINNKIKLIK
                     RTSFGYGSYNHLRNRILLCSKLYAPKSKKEVKQCSVA"
     gene            complement(245..565)
                     /locus_tag="RJE42_002483"
     CDS             complement(245..565)
                     /locus_tag="RJE42_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877874.1"
                     /translation="MIWRCGVMGGIARGIDAMPLQFYDMALGRCNVYYAVVTLLFHTV
                     TCICELLWKCCDIYCYTDESLCQDDIAMMNSNSVINHFLHAEISLILFGSMSNWTDAF
                     NIGS"
     gene            complement(845..1492)
                     /locus_tag="RJE42_002484"
     CDS             complement(845..1492)
                     /locus_tag="RJE42_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDV5877875.1"
                     /translation="MQQSDIISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYAELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLTLEGQIVRDADRLDAIGAIGVARTFQFAGHFGGPMWTEQMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMIYLKQFFTEWNYHD"
     gene            complement(1520..3004)
                     /gene="cls"
                     /locus_tag="RJE42_002485"
     CDS             complement(1520..3004)
                     /gene="cls"
                     /locus_tag="RJE42_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV5877876.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYANRSLWIKFKEGISQLLSPIL"
     gene            3170..3463
                     /gene="csoR"
                     /locus_tag="RJE42_002486"
     CDS             3170..3463
                     /gene="csoR"
                     /locus_tag="RJE42_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDV5877877.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            3475..3684
                     /locus_tag="RJE42_002487"
     CDS             3475..3684
                     /locus_tag="RJE42_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDV5877878.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            3812..3949
                     /locus_tag="RJE42_002488"
     CDS             3812..3949
                     /locus_tag="RJE42_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877879.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
BASE COUNT         1497 a          712 c          680 g         1290 t
ORIGIN      
        1 aaacgcattc agttgtccaa cgagcaatta aaacacttaa caaacatgca tactttataa
       61 aaaatacatt tgattattac aatttatcaa atggtccttt agaaggtata aataacaaaa
      121 taaaattaat taaacgtaca tcttttggtt atggaagtta caatcatttg cgcaatcgaa
      181 tattattatg ttcaaaactt tacgctccaa aaagtaaaaa ggaagttaag caatgttcag
      241 ttgcctaact tccgatattg aacgcatcag tccaatttga catagagcca aataatatta
      301 acgagatttc agcatgtaaa aagtggttaa taacagagtt gctattcatc attgcaatgt
      361 catcttgaca caatgattca tcagtgtagc agtatatgtc gcaacatttc cacagcaatt
      421 cacaaataca tgtaacagtg tgaaataata acgtcacaac ggcataatac acattgcaac
      481 gtcccaacgc catatcatag aattgtaacg gcatggcatc aataccacgg gctataccac
      541 ccataacacc gcaacgccat atcatagcac tacatcaaga cgcatcgtag taccagcgca
      601 tcaaagtagc atggcatgac accaagcttc cacaacagca catcaaagta acatggcacc
      661 aacaccaagc tcccacaaca gcacatcaaa gtagcatggc acgacaccaa gcttccacaa
      721 cagcacatca tagcactaca tcaagacgca tcgtggtacc agcacatcaa agtaacatgt
      781 catgacacca agttcccgca acaatacgtc attacatcgc atcatcatac tacaacttca
      841 atgtctagtc gtgataattc cattccgtaa aaaactgttt caagtagatc atcataaagt
      901 cgtgacgttc tttagcaatc atcttcgccg tatctgtatg cattaaagat tctaacttaa
      961 gtaatttttc aaagaaatgt ttaattgcag atggtggcaa ctgttcaact aaatcatcat
     1021 taatcttatc tagtgacatt tgttctgtcc acataggtcc accaaagtgt cctgcaaatt
     1081 gaaatgttcg tgcaacacct atagcgccta tagcatcaag acgatctgca tccctgacaa
     1141 tttgaccttc taaagttaaa gtgacatgat cgtttttacc attgcgatag ctcatattat
     1201 taataataaa taaaatgtgc tcttgatctt cggttgatag tgataaagaa gataaaaagg
     1261 acttcaattc agcatattgt ttgttagcat ctacaacttt ttcgtcaacg gtatcatgaa
     1321 gcaaacatgc gagttcaatg actaaagtat cattaacacc ttcattttca gcgattgatt
     1381 tagctaaagc agtgacacga tatacatgag caatatcatg gcctgtataa tcattttgat
     1441 gaatagattc catatatttt ttggctgcac taatgatgtc tgattgttgc attaaagtta
     1501 cactccccat atttctattt tataagatag gtgacaataa ttgtgaaata ccttctttaa
     1561 atttaatcca aagacttcga ttagcataac gtgcttttgt taattcagaa gatactgcta
     1621 aatcatctat aaaagcttgt tttaattttt tggcaatttg ttggtcataa ataaaggcgt
     1681 tgacttcgaa attcaatgtg aaactgcgat ggtccatatt agctgttcca acacttgcaa
     1741 tttcatcatc tataacaagt gtttttgagt gtaaaaagcc attgtcatag tgaaatactt
     1801 taacaccggc atctaataag gatgctgcat tttttaaagt agcccaaaaa acaaacggat
     1861 ggtcaggttt attaggaatc atgatattga catcgacgcc gcctaatgcc gcaattttaa
     1921 tagaatctaa aaaggcttga tcaggtatga aatagggaga ttgaatataa atcgattttt
     1981 tagcagatga aatcattttc aaataaccgt atttaatctg ttcccattct tcgtcaggac
     2041 cactagaagc aatttgaaca ccaattgttc caccagaatt tacatctggg aaataacgat
     2101 catcatagga gatgtggtca cgtgtggctt gtgaattcca atctagaata aatcgtaatt
     2161 gcaatgcatt cactgcatcc ccgacaattc gtaaatgcgt atctcgccaa tagccgaatt
     2221 tttttgattt acctaagtac tcatcgccaa cattaaaacc accaacatat ccaatttgcc
     2281 catctattac aacaattttt cgatggtttc gattgttcat acgcaagtta attaaaggta
     2341 attttgatgg gaaaaatgct tcagcatgtc cacctttatt gcgaaacggg cgtaagcctt
     2401 ttttacgcag tccacgagaa cccatgtcat cataaagaat tttaacttct acaccttgtt
     2461 ccgctttttt accaagttca tttaaaatgg tacgacctaa ttcatcattt tgaataatat
     2521 agtactgaaa atgaatataa tcagtagcat tacggatgtc ttgtattaag tcatcaaatt
     2581 tttcttggcc gtctgtgtat atttttaaat cgttgtctgt tgttaaaaat gctgcgttat
     2641 tatataacaa catttgaatc atttctttaa attttacaat ttgataattg gaatttgaaa
     2701 agttttcatt ttttaaagca gctaattgct catcaacgat taactctaat ccttttttat
     2761 cttccttatc aattttgaaa atttggtcac gttgaatttg tcgtcctaat agtaagtata
     2821 aaatgaagcc gaataaaggc aagaaaacta agactagtaa ccaagcccag atagaattgg
     2881 cagaacgtct ttccatgaaa ataatggtaa aggcgaataa taagtttaaa ataaatgcac
     2941 caataaatat tgaatttaaa ataatattag aatgcttgag tgcaatggat aataactcta
     3001 tcatttgaaa cctcccatcg aaaatcttaa acgagtagta tatcttatta attgtatcat
     3061 gaagcaattt aaatatcatg acaatttagt ttgaaaaaca cactttgtgt tgacgttgtg
     3121 ttttatggga tatagtatag taaggaatgt aaatgaagga gtgaatgcta tgactgaaca
     3181 agataatgca catcattctg aacaaataaa aacgaatctt aaatcacgtt taaatcgaat
     3241 tgaaggacaa gtgagagcga ttaatcgcat gattgaagaa gatgtctatt gtgatgatgt
     3301 ccttacgcaa ataagagcga cacgttcggc gttaaacagt gttgcgataa agttattaga
     3361 acaacatatg aaaagttgta ttatgaataa agttaatcaa ggagctcagg aagaggcaat
     3421 ggaagagtta ttagtgactt ttcaaaaatt gattaaagac taaaggagtt taagatgata
     3481 catcaaaata cgatttacac agcgggaatt gaaacagaag aacaagtaag tcaattgaca
     3541 gaacgcattt caaatatgat aggtgttcat caagtgaata ttaatataat tgatggtcaa
     3601 gtaacggtat cgtatgagac accagcaaat ttgaatagta ttgaaaaaga aatttatgat
     3661 gaaggataca aaattgtatt ttagggtata atgtagagtg cgctatagat tttaattttg
     3721 aaaataaatt aaaaattttg taaatgatgt agtaaaggta tgtcgaatag ggtaagataa
     3781 tattgaaagc gatccaaacg aggtgataat aatgaaacgt cctgaaaaga ttcaaaatgt
     3841 agtcaaacta ttgtcatcat taggtgtgaa tattaaaaaa actaaatctc gtttagacat
     3901 tattaatact ttgccagcat ctaataaagt aagtcacgaa ttaaaataat tgactgtgat
     3961 tgtgaatcac acaccgttaa agctatcgtc ggactattcg atgatagctt ttttgactgg
     4021 atttactaaa atgaaagcaa gtactatata acaaaatccc tctttaaagt gtacatttta
     4081 caatgtgtac ttcaaagagg gattgttatt atcgagtaat atctatatat gacattttaa
     4141 atcgacgaca taaatagcct atataatgtc atcttgaaa
//
LOCUS       DAPIDM010000177         4133 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000177 DAPIDM010000000
VERSION     DAPIDM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.179"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..192
                     /locus_tag="RJE42_002489"
     CDS             <1..192
                     /locus_tag="RJE42_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877880.1"
                     /translation="EDYSNKQYDIAKELKIGHVSLYRNLGNFDLTKTNELMYSDGVHP
                     NKIGGYAISNVIYDRLLRI"
     gene            248..685
                     /locus_tag="RJE42_002490"
     CDS             248..685
                     /locus_tag="RJE42_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDV5877881.1"
                     /translation="MDINWKLRFKNKAVLTGLIGALLLFIKQVTDLFGFDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVADSSIAQTYQTPRDSSKEEQQVTWKTSQDTSLT
                     PELSTKAPKEYDTSQPFTDASNEIGFDVNEYHHGGGNNASKID"
     gene            666..2111
                     /locus_tag="RJE42_002491"
     CDS             666..2111
                     /locus_tag="RJE42_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV5877882.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQYNKDGWYGFQCFDYGNAGWQALY
                     GCLLKGVGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGGNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQVGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKQLQLKDYFIKQIRTYMDGKIPVSTVSSDSSASSNTVKPVGSAWKRNSY
                     GTYYMEEKATFTNGSEPITVRTVGPFISCPEAYQFQPGGYCNYDEVMLQDGHVWIGYD
                     WQGARYYLPIRTWNGSAPPNYGLGNLWGTIS"
     gene            2715..2909
                     /locus_tag="RJE42_002492"
     CDS             2715..2909
                     /locus_tag="RJE42_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV5877883.1"
                     /translation="MLIETVKKLINSNLSAYEIGKKTGVDPATIRRIRRGERTVEKLS
                     FDSAEKLYNYQRSLENEEDK"
     gene            2924..3124
                     /locus_tag="RJE42_002493"
     CDS             2924..3124
                     /locus_tag="RJE42_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877884.1"
                     /translation="MFVTKEEFKNLNVKEVFESGKNFIKITDGRHAIYWVNDRYVVLD
                     HKKGDLYPQKAYPKYIKRKLAS"
     gene            3661..>4133
                     /locus_tag="RJE42_002494"
     CDS             3661..>4133
                     /locus_tag="RJE42_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877885.1"
                     /translation="MYKYLRKISVATIASFVLVGPVYSTVESGKANAETAKAVDKEMK
                     QREKIGNALLEEIDNSKEDFKDPSVTEKLKGQVRDYKNGVAERGKATITAKAGAKAIR
                     ATVNKIGEKAWDKLVAKIENTTGTKLVMFHYQSINKFCDILTGFEGNLAEGIATG"
BASE COUNT         1490 a          646 c          838 g         1159 t
ORIGIN      
        1 gaagattaca gtaataaaca gtatgacata gccaaagaat taaaaatagg tcatgtgagc
       61 ctatacagaa acttaggtaa tttcgattta actaaaacga atgaattaat gtattccgac
      121 ggtgtgcatc ctaacaaaat aggtgggtac gcaatttcaa atgtaatata cgacagatta
      181 ttaagaatct aatttaaagc taacctttcg aggttggctt tttattttgg ataaaaggag
      241 caaacaaatg gatattaact ggaaattgag atttaaaaat aaagcagtat taacgggatt
      301 gattggggca ttattgctat ttatcaagca agttacagat ttattcggat tcgatttatc
      361 aactcaatta aatcaagcta gcgcgattat aggcgctatc ctcacgctac ttacagggat
      421 tggtgttatt actgacccaa cgtcaaaagg tgttgctgat tcatctatag cacagacata
      481 tcaaacgcct agagatagta gcaaagaaga acaacaagtc acttggaaaa cttcacaaga
      541 tactagctta acgccggaat taagtacaaa agcaccgaaa gagtacgaca catcacaacc
      601 atttacagac gcctctaatg aaatcggttt tgacgtgaac gagtatcatc atggaggtgg
      661 caacaatgca agcaaaattg actaaaaaag agtttataga gtggttgaaa acttctgagg
      721 gaaaacaata taacaaagat ggttggtatg gatttcaatg ttttgattac ggtaatgcag
      781 gttggcaggc tttatatggt tgtcttttaa aaggtgtagg tgcaaaagat attccattcg
      841 ccaacaattt tgacggacta gctactgtat accaaaatac accggacttc ttagcacaac
      901 ctggcgacat ggttgtattc ggtagtaact acggtgctgg atatggtcac gttgcatggg
      961 ttatcgaagc aactttagat tatatcattg tatatgagca gaattggcta ggcggtggct
     1021 ggactgacgg aattgaacaa cccggttggg gttgggaaaa agttacaaga cgacaacacg
     1081 cttacgactt ccctatgtgg tttatccgtc ctaacttcaa aagcgaaaca gctccacgat
     1141 cagttcaatc tcctacacaa gcacctaaaa aggaaacagc taagccacaa cctaaagcgg
     1201 tagaacttaa aatcatcaaa gatgtagtta aaggttacga cctacctaaa cgtggcggta
     1261 atcctaaagg tattgtcatt cataatgacg caggaagcaa aggagcgaca gcagaagctt
     1321 atcgcaacgg attagttaac gcacctttat cgagattaga agcaggtatt gcgcatagtt
     1381 atgtatcagg taacacagtg tggcaagcct tagatgaatc acaagtaggt tggcataccg
     1441 ctaatcaagt tgggaataaa tattattacg gtattgaagt gtgtcaatca atgggcgcag
     1501 ataatgctac gttcttaaaa aatgaacagg caactttcca agaatgcgct agattgttga
     1561 aaaaatgggg cttaccagca aacaggaaca caatcagatt gcacaacgaa ttcacttcaa
     1621 catcatgtcc acacagaagc tcagtattgc acactggttt tgacccagta actcgcggtc
     1681 tattgccgga agataaacaa ttacagttaa aagactattt tatcaagcaa attagaacgt
     1741 atatggacgg aaaaatacca gtttcaactg tctcaagcga ttcaagcgct tcaagtaata
     1801 cagttaagcc agtaggcagt gcgtggaagc gtaacagcta cggcacttac tacatggaag
     1861 agaaagcaac gttcacaaat ggtagtgaac ctataactgt aagaacagta ggaccattta
     1921 tatcatgtcc agaagcttat caattccaac caggtggata ctgtaattat gatgaggtaa
     1981 tgttacaaga cgggcatgtg tggattggtt acgattggca aggcgcacgc tactacttgc
     2041 ctattagaac gtggaatggt tccgcgccac caaattatgg actaggtaat ttatggggaa
     2101 caatcagtta atatgttata attaatatcc accacatcat atggcaggta cttcggtact
     2161 tgcctatttt ttatgttata atgtaattac agattaattg tgagttccta cataattgtg
     2221 ggggctcgcc tacagcacag actgtctata gtaaagttgc cgaagagatt ctaaacgtat
     2281 ttgtaagtac gtggtcttta ctagttaacc gtatctttat tgatgcggtt atttttttat
     2341 gcaaaaaata cgaaaaaagt ttataaaaag tgttgactaa agggcaactg tcctgtataa
     2401 taagtagtgt agtaagggag gtgacaacat ggacaggcta gaaagagaag aaaaaaagac
     2461 aaatatcgca aaagccaaaa gcgaaatttt ccgaaactac actttaggct ttgcaacact
     2521 tgtctcaata ataaaaatgt tttactagag gggggttatc ccctccctca tattatatta
     2581 cgaggtgatg aaaatgacaa acttaactaa aacgagaata aaaatctttt taaattatct
     2641 aatcggaatt ttagctttaa tatttgcttt ctatataatc ttaaaataaa actttagcct
     2701 gtccatgaaa aattatgtta atagaaacag taaaaaaatt aataaactca aatttatcag
     2761 cgtatgaaat cggcaaaaaa accggcgtag acccagcaac tataagaaga atcagaagag
     2821 gcgaaagaac ggttgaaaaa ttaagttttg attctgcaga aaaactttat aactatcaaa
     2881 gatcacttga aaacgaagaa gataaataaa aggagctaac gaaatgtttg ttacaaaaga
     2941 agaatttaaa aatttgaatg taaaagaagt atttgaatca ggtaaaaact ttataaaaat
     3001 cacagatgga agacatgcaa tatattgggt aaatgataga tacgtagtac ttgaccataa
     3061 aaaaggcgat ttgtacccgc aaaaagcata cccaaaatat atcaaaagaa aattagcaag
     3121 ctaaataaat agataaccac gtcaattaag acgtggttat tttttaggtt tgcgcgtgtc
     3181 aaatacgtgt caatttagtt ctatttcttt agttttcttt ctaaagttaa tcgctcataa
     3241 accgcatagt tataggtttt tcagctatat accaagataa gatttatccc gccgtctcca
     3301 tatttgtagc ctacagcctt tgtggttgtg ggctttttat tttagagtgc acaaaaagcg
     3361 cacagaaaat gcacatgata caaaaagaca ccaccaaatt tagttggtag tgccttttaa
     3421 gtatttaact gtgaatcgtc tacacttaat tggacaaatt ctatattgaa attttcggga
     3481 taatcgtaat tgcgaaactg ttatatgtgt taaatatgat gtgaagtggc taatatagac
     3541 tgccagaata tggaaatgaa tcttgatgtc caaaagtgat tgaaagataa ttattatatg
     3601 atatgctgaa aaagggtgtt tattaaggga ttgtataagt tctaataagg agtttttaca
     3661 atgtacaaat acttaagaaa aatttccgtt gctactattg cgtcttttgt tttggtgggg
     3721 cctgtttatt ctacggtaga atcaggtaaa gctaatgctg aaactgctaa agcggttgat
     3781 aaggaaatga agcaaagaga aaaaattgga aatgctttgt tagaagagat agataatagt
     3841 aaagaagatt ttaaagaccc aagtgtaact gaaaaattaa aagggcaagt tagagattat
     3901 aaaaatggtg ttgcagagag aggtaaagct actataacgg caaaagctgg agctaaagct
     3961 atccgagcta ctgtaaataa gattggagag aaagcttggg ataaattagt tgctaaaata
     4021 gaaaatacta ctggcacaaa gctagttatg ttccattatc aaagtattaa taagttttgc
     4081 gatattttaa caggttttga gggtaattta gctgagggta tagctacagg att
//
LOCUS       DAPIDM010000178         4059 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000178 DAPIDM010000000
VERSION     DAPIDM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.180"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(441..1559)
                     /gene="mnmA"
                     /locus_tag="RJE42_002495"
     CDS             complement(441..1559)
                     /gene="mnmA"
                     /locus_tag="RJE42_002495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV5877886.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(1560..2702)
                     /locus_tag="RJE42_002496"
     CDS             complement(1560..2702)
                     /locus_tag="RJE42_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV5877887.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            3013..4026
                     /locus_tag="RJE42_002497"
     CDS             3013..4026
                     /locus_tag="RJE42_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV5877888.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRLQPVQPPTHELQNILTPREYTMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQLNEK
                     NTL"
BASE COUNT         1252 a          798 c          607 g         1402 t
ORIGIN      
        1 tttttacaaa attaatttcg tttaccccaa ctcgcattgc ctgtagaatt tctttacgaa
       61 attctctatg tttgggcccc gtccccaact ctcattgcct gttgaatttc tttacgaaat
      121 tctctgtgtt gatgtcccgc caacttccac attattgaaa tctgattttc cgtcagcttt
      181 tatgttgggc cccgtcccca acttccattg cctgttaaaa ttctttaaga aattctctgt
      241 gttgggcccc gccaactcgc acattattga aatctgactt tccctcagct tctgtgttgg
      301 gggcccgcca atataacatt gtagagccta ggacattgtg atgtcccaga ctctatcatc
      361 atgaattatt ctcatcaaaa aatgtctttc gtcatttcca acgctgaaac ttcaaataag
      421 taatttattg ttgtcattgt ttaaacaaca taatttaatt gaccttcatt tttgaacaca
      481 tcgtcaattg ttgctccacc aagacacaca tcaccttgat aaaaaacaac tgcttgtcca
      541 ggtgtgattg ctcttactgg ctcagcaaaa gtaacacgta gtgcatggtc gttttcacgt
      601 ttcacaaaaa ctttcgtatc tttttggcga tatctaaatt tagctgtaca ttcaaaacct
      661 tgatctaagt cattatcttc tggatttaca aatgaatagt ctgaagcaat taagtaatca
      721 ctgtataatg catcgtgatg gaatccttgt tctacatata aaacattatc ttttagattt
      781 ttaccgacaa caaaccaagg atctccatct ccacctatac ctaaaccatg tctttgtcct
      841 attgtgtaat acatcaaccc actatgttta cccattttct taccatcaag tgtaatcata
      901 tcacccggtt gcgcaggtaa atattgagat aaaaatgttt taaagttttt ttcgccgata
      961 aaacaaatgc ctgtagaatc ttttttctta gcagtaacaa gtccttgttc ttcagcaatt
     1021 cgacgcactt cactcttttc gatgtcacca attgggaaca tcacttttga aagttgttgt
     1081 tgagataatt gattcaagaa gtatgtttga tctttattat tatctacacc acgtaacatt
     1141 tcaacatgac catcttcatg acgatgtatg cgtgcataat gtcctgttgc tacataatct
     1201 gcacctaaat tcatcgcatg gtctaaaaag gctttaaact taatttcttt attacacata
     1261 acgtctgggt ttggagtacg acctttttta tattcatcta agaaatacgt aaagacttta
     1321 tcccaatatt ctttttcaaa attaacagcg taatatggaa tgccaatttg attacacact
     1381 tcaataacat cgttgtaatc ttcagttgca gtacatacac cattttcgtc agtgtcatcc
     1441 cagtttttca taaatatgcc aatgacatca taaccttgtt cttttaagac gtgggctgtt
     1501 acagaactat ctacaccgcc tgacatacca acgacaacac gtatatcttt atttgacaat
     1561 tatgactcct ccttaaattt aaaatatatt ttatgaattt cagctacaat tgcattaatt
     1621 tcattttcag tagtcaattc gttaaaacta aatcgaatcg aatgatttga tcgctcttca
     1681 tcttcgaaca ttgcatctaa aacatgcgac ggttgtgtag agcctgctgt acatgcagat
     1741 ccagacgaca catagatttg tgccatatcc aacaatgtta acatcgtttc aacttcaaca
     1801 aacggaaaat atagatttac aatatggcct gtagcatccg tcattgaacc atttaattca
     1861 aatggaatcg ctctttcttg taatttaact aaaaattgtt cttttaaatt cattaaatga
     1921 atattgttat cgtctcgatt cttttctgct aattgtaatg ctttagccat cccgacaatt
     1981 tgtgcaagat tttcagtacc tgcacggcgt ttcaattctt gttcaccgcc aagttgagga
     2041 taatctaatg tgacatggtc tttaactaat aatgcaccga caccttttgg tccgccaaac
     2101 ttatgagcag taatactcat tgcgtcgatc tcaaattcgt caaacttaac atcaagatgt
     2161 ccaattgctt gaaccgcatc aacatggaaa tatgcatttg tctcagcaat aatatcttga
     2221 atatcataaa tttgttgcac tgtgccaact tcattattta caaacataat agatactaaa
     2281 atcgtcttat ctgtaattgt ttcttcaagt tgatctaaat caatagcacc tgtatcatca
     2341 acatctagat atgttacatc aaaaccttct cgctctaatt gttcaaaaac atgtaacaca
     2401 gaatgatgtt caatcttcga tgtgataatg tgattaccca attgttcatt tgcttttact
     2461 atgcctttaa ttgccgtatt attcgattct gttgcgccac tcgtaaatat aatttcatgt
     2521 gtatctgcac caagtaattg tgcaatttga cgtcttgact catctaaata tttacgcgca
     2581 tctcttccct tagcatgtat tgatgatgga ttaccataat gcgaattgta aatcgtcatc
     2641 atcgcatcta ctacttcagg ttttactggt gtggtcgcag cataatctgc ataaatttcc
     2701 atgtttggac actcctcaca attttatcaa tgttccaata atagcacctt acatactatt
     2761 tttctacttt tctgtttaac tttatttata atgtttttaa ttatatttta ccattttcta
     2821 cacatgcttt tcgataggct ttttaagttt atcgctttat tcttgtcttt tttataaatt
     2881 ttagtatttg cagatatttt ttcatttgta aaatgtaacg tactattatt ttggttatga
     2941 gcaatttaat atttatctgg ttattcgatt ggtatacttc ttatatcaaa aaaaaggaag
     3001 gacgatataa atatggcgat taaatattca gcattaaacc ttgtccctat tcgagaaggt
     3061 gaagatgaac gaacagcaat taatgatatg gttaagctcg cacaacattt agacgaatta
     3121 tcatatgaaa gatattggat tgctgaacac cataacgctc ccaatctagt aagttcagca
     3181 actgctttat taattcaaca tacgttagaa catacgaaac acatacgtgt aggttctgga
     3241 ggaatcatgt tacctaatca tgctccatta atcgttgcgg aacaatttgg cacgatggca
     3301 acattatttc caaatcgtgt cgatttagga ttaggacgtg cacctggaac agatatgatg
     3361 accgcaagtg cattaagacg agatcaacat gatggtgttt ataaatttcc ggaagaagtt
     3421 tcattattac aacaatattt cggccccgct caccaacaag catatgttcg tgcttatcca
     3481 gcagtaggta aaaatgtgcc tttatatatt cttggttctt caacagattc tgcacattta
     3541 gctgctcgca aagggcttcc atatgtgttc gctggacatt ttgcacctca acaaatgaaa
     3601 gaagctatcg aaatctacaa aacgttattt gaaccttctg atgtattaga cgaaccttat
     3661 gttattgtat gtttaaatac aatcgttgct gaaaatgatg acgaagcaca atatttagct
     3721 tcatctatgg cacaagtaat ggttagtatc actcgtggca gattgcagcc cgttcaaccg
     3781 ccaacacatg aactacaaaa tatattaacg ccaagagaat atacgatggc tatggaaaga
     3841 cagaaaatat cattaatagg ttcagaaaat actgttcaac aaaaaattca agattttatg
     3901 gaaacttatg gtgaagtcaa cgaaattatg gctataagtt atatttatga taaagatatg
     3961 caattagact cttatcgtcg attcaaaaat gttataaatc agttaaatga aaaaaacact
     4021 ttataatgtg ataaataaac taagtgaaag tatgtatcc
//
LOCUS       DAPIDM010000179         4037 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000179 DAPIDM010000000
VERSION     DAPIDM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.181"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            12..473
                     /gene="tsaE"
                     /locus_tag="RJE42_002498"
     CDS             12..473
                     /gene="tsaE"
                     /locus_tag="RJE42_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV5877889.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENSRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            454..1116
                     /gene="tsaB"
                     /locus_tag="RJE42_002499"
     CDS             454..1116
                     /gene="tsaB"
                     /locus_tag="RJE42_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV5877890.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQKLDAIIVAEGPGSYTGLRIGVTVAKTLAYALNVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDDLQGDVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1089..1553
                     /gene="rimI"
                     /locus_tag="RJE42_002500"
     CDS             1089..1553
                     /gene="rimI"
                     /locus_tag="RJE42_002500"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV5877891.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1546..2571
                     /gene="tsaD"
                     /locus_tag="RJE42_002501"
     CDS             1546..2571
                     /gene="tsaD"
                     /locus_tag="RJE42_002501"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV5877892.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLTFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2888..>4037)
                     /locus_tag="RJE42_002502"
     CDS             complement(2888..>4037)
                     /locus_tag="RJE42_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDV5877893.1"
                     /translation="KRYNILFLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSAT
                     LKRTYEDDLKSIFYASNVLKQGYTISKIKHAPQPEVNFKQFRTARHLTSVLAVVNDED
                     IGAMVIKLVKLIFMLDYVLFHSIQKSYTTHMNELKNCFDYIAELDNHYSLAMYRRTLE
                     CYTEPQIDDSNDGIVFSELTHPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAIN
                     IILASAIQTVTAGKFVYQPGIVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKI
                     YCFIDEIFKGTNTTERIAASESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFN
                     EVIENNNIHFDYKIKPGKANTRNAIELLKITSFPAKIYERAKDNVSNG"
BASE COUNT         1373 a          587 c          776 g         1301 t
ORIGIN      
        1 gagaatcaac attgataaag ataaataatt tagatgaaat gaatcaattt gctatgtttt
       61 tagttgagca attgaaaagt ggtgatttga ttttacttaa cggagattta ggagcaggta
      121 aaacaacgtt aacgcaattt ataggaaaag ctcttggtgt aagacgtacg attaattccc
      181 cgacatttaa catcattaaa tcatataggg gtaaaaattt aaaattgcat catatggatt
      241 gttatcgctt agaagattct gatgaagatt tagggtttga tgaatttttc gaagatcagg
      301 caattactgt tattgaatgg agtcaattta taaaagattt actcccagcg acgcatttat
      361 ctattaacat ttcaacaata tctgaaaatt caagacaaat tgagttgttc gcgcaaggag
      421 aacattacga acaaattaag gaggcaatta tccatgaatt cgctgctcat tgatacatcg
      481 aatcaaccat tatcagtagc attgatgcaa aacgatgaag ttttagctga aataactacg
      541 gattctaaac aaaatcattc agtgcaatta atgcctgcga ttagtcagct atttgagcag
      601 agcaaaatag ctaaacagaa attagatgca attattgttg ctgaaggtcc tggttcatat
      661 actggcttac gtatcggtgt tactgttgct aaaacattag cgtatgcgtt aaatgttaaa
      721 ttatatggtg tttcatcatt aaaggcattg gcagcaacaa tcgatcatac tgataaatta
      781 ttggttcctg tgtttgatgc aagacgacaa gcagtttata caggcatatt ccagtggcaa
      841 aatggtcaat tagtgactat tttagaagat caatatatgt cgatagaagc attgcgagct
      901 tttttagaag atttaaacca accatttatt tatattggga aagatacagt aaaattacaa
      961 gatgatttac aaggtgatgt gattgcgcag ctaccaaatg catcagtcat gtatcaattg
     1021 atagataaac cttcagacgt acatacattt actcctaaat accataaatt agctgaggcg
     1081 gaacgaaatt ggatcaacag tcaaaagaac aattaaacat tagagagatg accaaagaag
     1141 atgtgccaca agtctttgat attgagcgtc ggagtttcaa tgatagttct tggacaattg
     1201 acgcatttta tcatgaaata gagcaaaata actttgcgaa atattttgtc ttagaatttg
     1261 aacaacaaat tattggttat ttaggtttat ggattgttat cgatcaagct caaattacga
     1321 cagttgcaat cgatgataat tatagaggat atggtttagg tcaaatgctt ttaaaatacg
     1381 gaataaacta tgcaagtcac acttgtgatg tgatgagttt agaagtaaga gttaataata
     1441 aagtggcaca acatgtatat gaaaatttag gatttcaata tggtggtaaa cgtaaaaatt
     1501 attatggtga aggtgaggac gcaatggtta tgtgggtgaa tttaaatgac taaagatata
     1561 ttaatactag ctgttgaaac aagttgtgat gaaacaagcg ttagtgttat aaaaaatggt
     1621 agagatattt tatcaaatac agttttaagt cagattgaaa gtcataaacg atttggcggt
     1681 gtcgttcccg aagtggcaag tagacatcac gttgaaggta taacaacaac aataaacgag
     1741 gctctagtgg atgccgacgt atcaatggaa gatattgatg ccatagcggt tacagaaggc
     1801 cctggactaa ttggtgcgtt actaataggt gttaatgcag ccaaagcatt ggcatttgct
     1861 tacgataagc cacttattcc tgttcatcat attgcaggac acatatatgc taatcacata
     1921 gaagagccat taacattccc gttaattgca cttattgtct caggtggaca tactgaatta
     1981 gtttatatga aagatcattt aacatttgaa gtcattggtg aaacacgaga tgacgcagta
     2041 ggtgaggctt atgataaagt ggcacgaaca attggtttaa attatccagg tggtccacaa
     2101 gttgatcgtt tggcagctga aggtgaagat acttattcat tccctcgtgt ttggttggat
     2161 aaagatagtt atgactttag ttttagtggg ttgaaaagtg ccgtaatcaa tcaacttcac
     2221 aatcaacgac aaaaaaatat tccaatcatt gaagctaacg tagcaacgag ctttcaaaac
     2281 agtgttgtag aggtgcttac gtttaaagct attcaagctt gtaaagaata tggtgttcag
     2341 cgattaattg ttgctggtgg cgtggcgagt aataaaggat tacgtcaatc tttagcggat
     2401 caatgcaaag tcaatgacat tcaattaact atcccaagtc ctaaattatg cacagataat
     2461 gctgcaatga taggtgttgc cggccactat ttgtatcagc aaggtcgatt tgctgattta
     2521 gcattaaatg ggcacagcaa tatagattta gaagagtatt ctgcagaata aaataaacgt
     2581 cagttattaa catgattacg atgaacatta ttgaagctgt tcatagtctt ctgtcatgtg
     2641 atagctgacg tttttatatt gtaattaggg gagtgagaca gaaaagataa agaaccacta
     2701 atgatttatt atgttggggc ccacacccca acttccattg cctgtagaaa ttggaaatcc
     2761 aattcctctt tgttggggcc cctgactagt attgaaaaaa cttgttacaa gcgcattttc
     2821 atttagtcaa agactgtcaa tatagcattg tagcgacttg atcattgatt gatgttcaag
     2881 attcatgcta accattggaa acattatctt ttgctcgttc atatattttt gctggaaatg
     2941 aagtgatttt taataattcg atggcattac gtgtatttgc tttgccaggc ttaattttgt
     3001 aatcaaaatg tatgttatta ttttctatta cctcattgaa atgataattt tcataacgtt
     3061 gttttaagag ttcagctaac tcaatatcat gtgttgctgc aataactcta aagttagatt
     3121 tttcatgtaa aaatgatagt actgattctg aagcggcaat tcgttcagtt gtgttggtac
     3181 ctttaaaaat ttcatcgata aagcagtata ttttttgatt atcaggaatt tcaacaatac
     3241 gcttaataga ttttaattca gccataaaat aactatcgcc tgataacaca tcatcggcat
     3301 ttgccattga cgtgaacaca attccaggtt gatatacaaa tttgcctgcg gtaactgttt
     3361 gtatagccga tgcaagaatg atgtttattg ctatggattt cataaatgtt gatttacccg
     3421 aagcattaga tccagttaac agtatatttt gtgatagtga gaaatcattg gctactgcat
     3481 ctgctatgag tggatgtgtt aactcggaga atactatgcc atcatttgaa tcatcaattt
     3541 gtggttcagt atagcattct agcgttctac gatacattgc taatgaataa tgattatcta
     3601 attcagctat gtaatcgaaa caatttttta gctcattcat atgtgttgta tagctttttt
     3661 gaattgaatg gaataaaaca taatcaagca tgaaaataag tttaactagt ttaattacca
     3721 tagctccaat atcttcatca ttaactacag ctaaaacact ggttaaatga cgtgctgttc
     3781 taaattgctt aaaattgact tcaggttgtg gagcatgttt aatttttgag atagtatagc
     3841 cttgttttaa aacattggac gcataaaaga tagactttaa gtcatcctcg tatgtacgct
     3901 ttaaagttgc agataaaatg atattaaata ggcaactcat aagaaataat aaaataccaa
     3961 cttgtgaatt aataaatata actgcaaatg agataactgg taaaaacggg catagaaaca
     4021 atatattata acgtttt
//
LOCUS       DAPIDM010000180         4009 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000180 DAPIDM010000000
VERSION     DAPIDM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.182"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(289..738)
                     /gene="ndk"
                     /locus_tag="RJE42_002503"
     CDS             complement(289..738)
                     /gene="ndk"
                     /locus_tag="RJE42_002503"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV5877894.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            complement(830..1789)
                     /locus_tag="RJE42_002504"
     CDS             complement(830..1789)
                     /locus_tag="RJE42_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDV5877895.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            complement(1791..2516)
                     /locus_tag="RJE42_002505"
     CDS             complement(1791..2516)
                     /locus_tag="RJE42_002505"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDV5877896.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVEPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            complement(2519..3091)
                     /locus_tag="RJE42_002506"
     CDS             complement(2519..3091)
                     /locus_tag="RJE42_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDV5877897.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            complement(3522..3794)
                     /locus_tag="RJE42_002507"
     CDS             complement(3522..3794)
                     /locus_tag="RJE42_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDV5877898.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1254 a          729 c          525 g         1501 t
ORIGIN      
        1 tctaaaaata tagcaccata tagtaattcc attaatttta agaacattaa aatccccaaa
       61 ataatcgaaa taatattctt cattttaaca accccttctc tatgtttagt ttcttttgat
      121 aaagaatatt tctctctttt taatacgtgt taccaaaaaa actataaaat ttttcaagta
      181 atttactaca acataaaaaa cgggccttgt tatgtataat cataatgact aaccacataa
      241 caaaaccctt tttcctttta taaatcagaa agatttatag cttatatttt attcgtataa
      301 ccaagcatca cgtggtgaag catagctagt aatttcattt tcattaaacc ataaattaat
      361 ttcacgttca gcagactcta atgaatctga accgtgaatg atatttctac caacagttaa
      421 acctaaatca cctctaattg atcctggtga agcttctgaa ggattggtgc tgccaataat
      481 atgtctagat acattaactg catcttcacc ttcaactacc attgcgaaca ctggtgctga
      541 tgtaataaat gaaattaaat cattataaaa tggtttacct tggtgttcac cataatgtgt
      601 ttcagcaagt tccattggta cttgcattaa tttaccaccg acaagtttta gtccttttct
      661 ttcgattctt gaaattactt caccaattag atttctttgt actgcatctg gtttaatcat
      721 taaaaatgta cgttccactt tatgtatccc cctgtgttta ataggtttat gtatattact
      781 attatatatt aacaggttgg taaagcgctt tcaatacttt tcataaatac tatgtgtttc
      841 ttgaacccat ttttttcgtc aaacttaaaa gtagtgattt cggatgtcca tctggtaact
      901 cagaaatcaa atctaaagct ttacttaaat acttcgaact tactgcctta gcctcatcga
      961 tgctgtcaga ttttctaatg atttggatac attcttcaaa ttctttgcgt tcactatcac
     1021 gacgtaattg ttcgattttc aatttgaagt ctggattttt acgcatttct aataaaatcg
     1081 gtaacgtaat atgaccatta agcaaatcac ttccgaccgg cttacctaat ttcttttcgg
     1141 tacttgtgaa gtctaataca tcatcaatga tttggaagct catacctata tagtgaccaa
     1201 tcattttcaa ttttcgtaca gtctctttat cagattgaga agtaattgca ccaacttcag
     1261 ttgataattg aattaacagt gctgttttgc gattgatacg tcgtaaataa ttgataattg
     1321 tctgttgact gttaaattgg tcttgaaatt ggaaaagttc ccctctacaa acatcaacga
     1381 tagattcaga tattaattga tgtacacgat tatctttaac ggccattaag tgttcaagtc
     1441 ctaatgccaa taaaaaattc ccagttaaaa tagcagttgt ctgatcccat ttctttgata
     1501 tggttaactt gcctcgacgc ttgtcgcttt tatcaataac gtcatcatga acaagtgttg
     1561 ccatatgaat taactctaat gcgactgcaa cttgatacgt ttgttcagac gtttgttcat
     1621 ctttgccaaa ttggctactc agaattacaa atgctgggcg tactctttta ccaccagaag
     1681 acaataagtg taatgatgcc tgttctaata cagaatcttt actttttatt gccttttcaa
     1741 gtcgttgttc cactttctta atttcattgt tcatgtttaa ctttgccacg ttaatcacct
     1801 ttggtattat ctttttcttt atagccaagg tgcattgcag caacgccccc tgtaaaacta
     1861 cgtactctta catttatgaa acccgcttct tcaaacatgc gcttcaactc ttcttttcca
     1921 ggaaaattaa acgtagattg ctgtaaccat tcatattctt cttttgattt tgcaaataat
     1981 tttccaaaaa taggcataac aaatttaaag tataatgcat acatttgttt aaagactggc
     2041 aaagttggtt ggctcgtttc aagacatacc accataccac ctggtttaag tactctattc
     2101 atttctttta acgcgactaa atagtctggc acattcctta atccaaaccc aattgttaca
     2161 taatcaaaag aattgtcttc aaacggcaat tccattgcat caccatgaac aagtttaaca
     2221 ttttccattg aagcagtttt ttcttttcct acttctaaca tattctcact aaagtcaata
     2281 ccagtaactt ctcctgttgg ttctacagct ttgcttaatg cgattgtcca atcaccagta
     2341 ccacaacaaa catctaatgc tttcgtacct tttctaacac ccatgtcttt catgacgcgt
     2401 tttctccata ctttatgctg ctcaaaacta ataatattat ttaatctgtc atattttttt
     2461 gaaatatttt gaaaaacgcg atgtacttgc tctttatttg ctttattgtc agccatgctt
     2521 aattacctct actttttaaa taactttttt ggatatcgtg taagtaatgc tttacttcac
     2581 tttgattata tttcttgaag tatgatggat agtaatcaga catatcttca aataaataat
     2641 tatatatttc cgactcatca atattgatac cgaaatgaga taacgtaata tatgggaaaa
     2701 gtgtttcaat ttttactatt gcttgagaaa tttcataatc atttaaagct tgatgatgta
     2761 atgaagattt caattcatta atttcaacaa ttgctttact aatttcattt tgaaatgata
     2821 aatcattgat ttctgctagt agcgtataaa aatgtgcact aattaaatct ccaatcaaaa
     2881 tggaatgctt agacaaatga ttatatgtaa tgtcatcaag gtgtctcatt gatgtgtcaa
     2941 ttgtgaggca tgctactttg gcaacatctg gaatatcata tgaatcgagt aacttaccta
     3001 accgatgatt aatattaatg gattcatatt ctgatacgcc ctttaatctt tcttctattt
     3061 gtctttccaa tttgctaaca gttgtttcca tgtctacacc tcacgttatt atcactattc
     3121 atattaacat tatatgtaag aaattaaaat cttttgaagc attaagatta cttatcattt
     3181 ttaaatttca atttaaacta acagtaattt atgtagcttt tgtaattctc ataataaccc
     3241 ttacttcatt tacattattc atcattttaa ataacgtaaa atactatagt agacatggtt
     3301 accttttaag taaattcaac gctaacaatt aatctcatta ttcatatgta tattttgtag
     3361 ttgctatttt tatcaaaact atgatgtcat gtaatatttt gaacgcttaa acaatgaatg
     3421 ttccaaagat taaaaactta gaagaacatt acaatttatg tacaggttgt aagtattaaa
     3481 agcaaaataa aaagccctta ttcagggctt tttaagtaaa attattttac agcatctttt
     3541 aatgctttac cagctttgaa tgctggaact ttacttgctg ggatatcaat ttctttacca
     3601 gtttgagggt tacgaccttt acgtgcagca cgttcacgta cctcaaagtt accgaaacca
     3661 attaattgta ctttttcacc tttagcaagt gagttttgga ttgattcgaa tacagcatct
     3721 actgctgaac cagcttcttt tttagttaaa tcagcttgct ctgcaactgc attgattaaa
     3781 tctgttttgt tcattagaca ttcacctcct gaggtttgat aatgatatgt ttatatcatt
     3841 atggtaaaac tttaacacaa gcattacata aatcgcaatc atttatgtgg caaaaacgtt
     3901 gaatttaagc catttctatt cattttacgt aattattagc tgtatatgtg taataataca
     3961 cattttattc agatttttta tcgcgctcca ttaaatcttt tacgcattc
//
LOCUS       DAPIDM010000181         3975 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000181 DAPIDM010000000
VERSION     DAPIDM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.183"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..2603)
                     /gene="smc"
                     /locus_tag="RJE42_002508"
     CDS             complement(<1..2603)
                     /gene="smc"
                     /locus_tag="RJE42_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDV5877899.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGQ
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLIELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLNDVKEKIAF
                     FNSDEVMGEQA"
     gene            complement(2750..3481)
                     /locus_tag="RJE42_002509"
     CDS             complement(2750..3481)
                     /locus_tag="RJE42_002509"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV5877900.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(3597..3830)
                     /locus_tag="RJE42_002510"
     CDS             complement(3597..3830)
                     /locus_tag="RJE42_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV5877901.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
BASE COUNT         1129 a          748 c          520 g         1578 t
ORIGIN      
        1 aaagcttgtt cgcccatcac ttcatccgaa ttaaagaatg ctattttttc tttaacatca
       61 tttaattgat gtttggtttg ttgactttga ttatttaatc gatctattgt ctgttgttgt
      121 gttttaatac gctctttaac cacagcaaga tcagattgtt tctgatgtaa cgtttgttga
      181 gttttagtaa cgctttcctt accttcttta gaaagtttag tgtagcgttc aatttcatct
      241 tctagtcgtt ttaaagatgc tttaatactt tctaaatgag tttctttttc actcaaagtt
      301 tgtcgacttt tgtcactcgt ataaccatca tttttttcaa attcaaattc ttcatgatca
      361 ttttttattt gtgtttcttg tgtagttaat ctatcgagct ccatttcaaa atgatgcact
      421 tgctctttaa gtgtattatg cttttgactt ttttcaaaat acagttcact taattgatca
      481 ctttttatct tcaactcttt aaattgttgt tcaaatgatt ctgtttgacg caagtaatct
      541 tctaattggt gtctcattgt tgtcaactcg tctttttgag acagaatact ttttgactta
      601 cgagcgccac caccagtcat agaaccacca ggatttacaa tatcaccttc caaagtaaca
      661 atacgagttc gatatttaat cgcacgtgcc aattcatttg catgctttaa atgatcaacg
      721 ataatcgtat tacctaataa attcccgata atattttgat attctggtgc tactttcact
      781 gcttccgaag caatactaat aaatccgttt gcctctttag caatagactt aatatcagtc
      841 gctaccactc tactctgtat aacatttaat ggtaaaaacg tcgcacgacc taaattacgt
      901 tcttttaaaa attgaatagc ctggcgtcca tctttttctg aatctacaat gacatgttgt
      961 aatgaagcac ctaatgctgt ttcaattgcc tgagttaatt tagatggcac atcaataatt
     1021 tccgcaactg caccatgaat accctttaat tctttatttt tagctttcaa aatatgtttg
     1081 acgccattga aaaaataagt atattcttcc tcttgcgttg ccaaactatc aatacgtgtt
     1141 ttcatttttt cggtatatcg atatgcttga tacaatttct cttcgtattc attttgtgct
     1201 tttttagtat cagtgaggtc tttttctata tttttaattt ctttatctac agcagaaagt
     1261 tctttattgg tctgttggta ttctttttta gtcgttttta tttgaccctg aatatctttc
     1321 aattgctcaa agacttcaac taatcgagaa tctagtcttg attttttagc ctcattctct
     1381 tctatagtat gctttaaaaa acgaatatca ttgttaacat ctgattgctc tgacattaat
     1441 gtatagtatt cgtttttaat ttcttccaat ttttcatcgt gtgcttcgtc tgaaacatat
     1501 agttgttctt ctaattcacg aatgacagcg ttgagttctt tttgtttact tttcagagac
     1561 ttataagtat cttgagcttc agaaatctca tttgatatat tttctaaaag ctcaattaaa
     1621 ttttcttgtt cttcttcata tcgtgcattt gtttcagatt gattcttctt acgttcttct
     1681 aaaacattta attgtcccgt atatttttca aaagcttccg tagcttttac taattgataa
     1741 ttaagcgatt caacatcatt atcaagttga tgacgttgac ctttatattg ttgaatttgt
     1801 tggcttaaac gttgcttatc agcttcttta tttgcttgtt gaccttgtaa atcatttaaa
     1861 cgttgatcta attgtctatt gtcatttgta tattgatcaa tatcgtgaac tgtaactaca
     1921 atgtcactat gtttcatttg atgtgaaagt gtcttatatt ctttagctat agctgcctcc
     1981 tcttttagag gttctacacg accttccaaa tcatataaaa tgtcttctac tctcgttaaa
     2041 ttatcttctg tttggtcaag tttatttaat gattcagctt tacgtttttt atatttaagt
     2101 acaccagcag attcttcaat aatttgacgt ctatcaattg gtttagcatt tagtatttca
     2161 tcaactctac cttgcgagat aatgctatat gcttctttcc ccaatccaga atctaaaaat
     2221 aaatcggcaa tatcttttaa tcttgcacgg tcattattta tgtagtactc actttcacca
     2281 cttcgataca atcttcttgt tacaataact tcgttttcat caacactgag ctttttagaa
     2341 tgattatcta atcttaactg tacttcagca taattttgag ctttgcgatg ttctgcacct
     2401 gagaagataa tatcttccat ttttgagcca cgtaatgatt tagccgattg ttcacccaac
     2461 acccatttaa tagcatctgt aatattactt ttaccgcttc catttggacc aacaattgca
     2521 gttacacctt tatcgaattg aacattggtt tgatctgcaa aagacttaaa tccaatggca
     2581 tctattgatt ttaaataaac catcctaaac tccttatcac aattttaaag aacatacgta
     2641 tacgatattt tagtatttgt atattaaatt tcgaggttgt tccataaaat attaaaaatt
     2701 cttccataaa tcctaaatcg aacacctata tgtcgtatca taaaaatttc tatttaattt
     2761 gctttaattg cttataggca ctttcagcag cacgttgttc tgattctttt ttcgttttcc
     2821 ctttaccttc agctattgct tccccttgca gaataacttc tgaagtgaat agacgatgat
     2881 gtgccggtcc ttcttctttt attaaattat aggttacatc acctttattt tgctggtgca
     2941 catattcttg gaattgtgtt ttaaaatcta ccacgcctaa taactcattt tgttctacat
     3001 gtgggaaaat gactttctca gcaaatttcc aaactatatc tagtccttga tccaaataca
     3061 atgccccaat aaatgcttcg aatgcatctg atattaatga tggtcttgta cgtccccctg
     3121 ttttctcttc acctttacca agtaaaatca tttcgttcaa tccaatttta ttcgcaaata
     3181 ttacaagtga gggctcacat acaatagtgg cacgcatttt tgttaaattc ccttctggca
     3241 agttgggatg tttatcaaat aaatatcgtg aaaccgtcaa ttctaatacc gcatcaccca
     3301 aaaactctaa acgctcatta tggtctaaac gattcatatt aaaatcatta ataaaactcg
     3361 aatgcgaaaa tgcttgttgg tataaatcaa tattttgata agtaaagcct aactctgtca
     3421 ttttagtatc aaagcgcttt ctaaaacgat taactatctc acttttcttt tgtttagaca
     3481 ttattttatc tcctttatat gttgctcttg aatcatcttt aattttacgt taaaactatt
     3541 gataattaaa gaaaaaaact gagtcgacaa tactgacgac ccagatgtaa gatttattat
     3601 ttttcaagac tgttaataaa tttaacagca tcaccaacag tgttgatttt ttcagcttct
     3661 tcatcaggaa tttcagtacc aaactcgtct tctaattcca ttactaattc agcgatatca
     3721 agtgagtcag cgcctaaatc atctttgaaa gatgcatctt cagttacttt atcagcgtct
     3781 acacctaaac ggtcaacgat gatatctttt actttatcga aattttccac gtcgattcac
     3841 ctcctttaaa aatgcctata catagactat tatattttcc cattttaaat tctaaaaaac
     3901 aagtagaaat tttagaaaca gttatcaatt tattgattac tgtttcttat gcatgagctt
     3961 tagctcaggt gttcc
//
LOCUS       DAPIDM010000182         3885 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000182 DAPIDM010000000
VERSION     DAPIDM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.184"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..342
                     /locus_tag="RJE42_002511"
     CDS             <1..342
                     /locus_tag="RJE42_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDV5877902.1"
                     /translation="YSSRSLKEYRKYYEPLIRKNDKEFKEGMERARKEVNYAANTDAV
                     ATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFYDDNR
                     PIEYGVHSKDE"
     gene            335..2497
                     /locus_tag="RJE42_002512"
     CDS             335..2497
                     /locus_tag="RJE42_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877903.1"
                     /translation="MNKINDRDLTELSSYWVYQNIDIKKEFKVNGKRFKQVDSYNDDK
                     NSNLNGAADIKIYELLDDKSKPTGQQTIIYQGTSNEAINPNNPLKSSGFGDDWLQNAK
                     LMNNDNESTDYLKQTDQLSNQYKKKLEDADRLSNSDFLKKYRMESSNFKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDPAMVPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGRGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKKQLSEGFKDKINLFQLEEMERTLISKINSL
                     EEVADETIESISAVKHLLPDFALDALKERINELFKGIKSFIEKVYDSIDNEILEIFKN
                     IDHDFKDGVSEEMMKHLKVVKQNIQQIKNQNDIYGRQIADIRSIMKQQDATILDGNYQ
                     INYSCENMVQGLALPSNYLGRKMKILKDHIDDGIKKIADYVQGIYDEYASKIVDVIKY
                     LINTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKTKLEELDNTLGKWEPFLND
                     LKAVSPILDNHLDDIVKNMKPLIVQMIFEPSHYDDMFILNTQAHARLDQMAQQFEVVC
                     NGLNENEGQAIQTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            2588..3517
                     /locus_tag="RJE42_002513"
     CDS             2588..3517
                     /locus_tag="RJE42_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDV5877904.1"
                     /translation="MFKKAKIILIVTLLLSGCSAMENESSKDTNIETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKTTNVVGSGDGVEVYVH
                     CDDHDIVFNASIPFDKSIIESDSSLRSEDKSGDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKDNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYELLIRKNDK
                     EFKEGMERARKEVNYAADTDAVTTLFSTKKNFTKDNTVDDVIELSDKLYNFKNKPEKS
                     TITIQIGKPTINTKKAFYDDNHPIEYGVYRKDE"
     gene            3572..>3885
                     /locus_tag="RJE42_002514"
     CDS             3572..>3885
                     /locus_tag="RJE42_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDV5877905.1"
                     /translation="MFKRIKLILIATLVLSGCSTMEDKTNKETKSVPEEMEASKYVGQ
                     GFQPSAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIV"
BASE COUNT         1623 a          456 c          708 g         1098 t
ORIGIN      
        1 tactcttcta gaagtttaaa agaatatcga aagtattatg aaccactgat tcgaaaaaat
       61 gataaagaat ttaaagaagg aatggaacga gcaagaaaag aagtgaatta cgctgcaaat
      121 acagatgctg ttgctacact tttttctact aagaaaaact ttactaaaga caatacagta
      181 gatgatgtaa tcgaactaag tgacaaatta tataatttaa aaaataaacc agataaatct
      241 acaatcacaa tacaaatagg gaaacccact attaatacta agaaagcctt ttatgatgat
      301 aatcgtccaa tagaatatgg ggtgcacagt aaagatgaat aaaattaatg atagggattt
      361 aacagaatta agtagttact gggtttatca aaatattgat ataaaaaaag aatttaaagt
      421 taatggaaaa aggtttaaac aagtagacag ttataatgat gataagaata gtaatttgaa
      481 tggtgctgct gatattaaaa tatatgagtt attagatgat aaaagtaaac caactggtca
      541 acagacaata atttatcaag gaacatctaa tgaggcaatt aatccaaata atccattaaa
      601 atcatcgggg tttggagatg attggctcca aaatgctaaa ttaatgaata atgataatga
      661 aagcacagat tatttaaagc aaacagatca attatcaaat caatataaaa aaaagttaga
      721 agatgcagat agattatcaa atagtgattt tttaaaaaaa tatagaatgg aatcaagtaa
      781 cttcaaaaac aaaaccattg tggcggatgg cggtaattcg gaaggcggtg caggagcaaa
      841 atatcaagga gcgaaacatc caaatgaaaa agttgttgct actgacccag caatggtacc
      901 ttatgctgct tggcagaaat ttgctagacc acgctttgat aatatgatta gttttaatag
      961 taccaacgat ctattaacat ggttacaaga tccattcatc aaagatatgc cgggaaaacg
     1021 cgttaacatt agtgatggtg tgcccaggtt agatgcttta atagacagcc atgtaggtta
     1081 taaaaggaag ttaaatagaa aagataacac atacgatact gtaccactaa tcaaaattaa
     1141 gtcggtaaaa gatacagaaa ttaaaaatgg aaaaaaagta aaaaagacta ttaacataac
     1201 attagatatg gatgggcgaa ttccaataaa tgtttggaca ggagattcga ttgcacgttc
     1261 tggaagagga actttaatta aacttaattt agaaaatctt gatgcgttga gtaaactgat
     1321 tactggtgaa actagtggta tgttagcaga atgcgtaatc tttttaaatg aaagttttaa
     1381 catctcagaa aatgaaaata aaaattttgc agatagaaag aaacaattat cagaaggatt
     1441 taaggataag attaacttat ttcaattaga agaaatggaa agaactttaa ttagtaaaat
     1501 aaactcactt gaagaagttg cagatgaaac aatagaaagt attagtgctg ttaaacactt
     1561 attacctgat tttgcattgg atgcattaaa agaaagaatt aatgagttgt ttaaaggtat
     1621 aaaatctttt atagaaaaag tgtatgatag tatagataat gaaattttag aaattttcaa
     1681 aaatattgat catgacttca aggatggagt atctgaagaa atgatgaaac atttgaaagt
     1741 agtaaaacag aatattcagc aaataaaaaa tcaaaatgat atttatggta ggcaaattgc
     1801 agatattaga agtattatga agcaacaaga tgcaacaatt ttagatggaa attatcaaat
     1861 taattatagc tgtgaaaata tggtgcaggg tctagcttta ccttctaatt atttaggaag
     1921 aaaaatgaaa atattaaaag accatatcga tgatggtatt aaaaaaatag cagactatgt
     1981 tcaaggtata tatgatgaat atgcatcgaa aattgtcgat gtaataaaat atttgattaa
     2041 tacaattccc aaaatacgta agaatttaag acatgcaatt gaaatgttaa atgtaaaaaa
     2101 gaaagaattt ttgtccctga ttcctaatgt aacttgtaat tatattaaaa ctaaattaga
     2161 agaattagat aatactttag gcaaatggga gccttttctt aatgatttaa aagcagtgtc
     2221 accaatttta gataaccatt tagatgatat tgttaagaac atgaagcctt tgattgtaca
     2281 aatgatattt gaaccatcac attatgacga tatgtttatt ttaaacacac aagctcatgc
     2341 gcgtcttgat caaatggcac aacagtttga agttgtttgt aatggcttga acgaaaatga
     2401 aggacaagca attcaaacga tggatcaatc cgcctctcta atacggtcaa acttaattca
     2461 agttaaagaa caattagaaa aactagctgt atactaatta atttattaaa tgctacttgt
     2521 tttcattgag aataagtagc tttttcaaac ataaaagttt tacaaacaca taaatgggtg
     2581 atgagctatg tttaaaaagg cgaaaatcat attaatagta acactattgc tatcaggatg
     2641 ttccgcgatg gaaaatgaat caagtaaaga cacgaatata gaaacaaaat cagtaccaga
     2701 agaaatggaa gcttccaaat atgtaggtca aggcttccaa ccacctgcag aaaaagacgc
     2761 gattgaattt gcgaagaagc ataaagataa aattgctaaa cgtggcgaac aattttttat
     2821 ggataacttc ggtctaaaag ttaaaactac gaatgttgta ggtagtggcg acggcgtaga
     2881 agtatacgtg cattgtgatg accacgacat cgtatttaat gcgagtattc catttgataa
     2941 atcgataatt gagagtgata gctcattaag aagtgaggac aaaagtggtg atatgagtat
     3001 gatggtgggt acagtgttga gtggctttga atatcgagcg caaaaagaaa agtatgataa
     3061 cttatataaa tttttcaaag ataatgaaga gaaataccaa tatacaggct ttacaaaaga
     3121 agcaattaac aagacgcaaa atgttggata taaaaatgaa tatttttata ttacatactc
     3181 ttctagaagt ttaaaagaat atcgaaagta ttatgaacta ttgattcgaa aaaatgataa
     3241 agaatttaaa gaaggaatgg aacgagccag aaaagaagtg aattatgctg ctgatacaga
     3301 cgcagtaacg acattgttta gtacaaagaa aaattttact aaagacaata cagtagatga
     3361 tgtgattgaa ctaagtgata aattatataa ttttaaaaat aaaccagaaa aatctacaat
     3421 tacaattcaa atagggaagc ctactattaa tactaaaaaa gccttttatg atgataatca
     3481 tccaatagaa tatggggtgt atcgtaaaga tgaataaaat taaaaaaatg cttttaatat
     3541 tctaaacaaa cacataaatg ggtgatgagc tatgtttaaa aggattaaac taatcttaat
     3601 agcaacacta gtactatcag gatgttccac gatggaagat aaaacaaata aagaaacaaa
     3661 atctgtacca gaagaaatgg aggcttcaaa atatgtagga caaggctttc aaccatctgc
     3721 agaaaaagat gcgattgaat ttgcgaagaa gcatcgtaaa gaatttgaaa aagtaggtga
     3781 acaattcttt aaagataact ttggactgaa ggtaaaggct accaatgttg taggtaaaga
     3841 tgatggtgta gaagtttatg tgcattgtga agatcatggc attgt
//
LOCUS       DAPIDM010000183         3865 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000183 DAPIDM010000000
VERSION     DAPIDM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.185"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(129..1634)
                     /locus_tag="RJE42_002515"
     CDS             complement(129..1634)
                     /locus_tag="RJE42_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDV5877906.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVILAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(1974..2474)
                     /gene="queF"
                     /locus_tag="RJE42_002516"
     CDS             complement(1974..2474)
                     /gene="queF"
                     /locus_tag="RJE42_002516"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV5877907.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEQMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(2494..3360)
                     /locus_tag="RJE42_002517"
     CDS             complement(2494..3360)
                     /locus_tag="RJE42_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV5877908.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGKLSSQPLLLTRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQTQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKVIKK"
BASE COUNT         1393 a          600 c          607 g         1265 t
ORIGIN      
        1 attgctgtat ataacgagca tattaagttt ttatttatac tgatatcttg catttaatta
       61 atagaaacca ataaaaaaac agtaagccat ttaaatgact tactgttttt tgaattaggc
      121 caacaatatt aacgaatacc tttcattgct ttaataatta aaggtgagaa tgctaataca
      181 attgttgtaa caataattgc aacaacacct aagaagataa agtaatttgt ttgacctagt
      241 ggttctatta acttaactaa tgtaccattg attgcttgtg cagaagcgtt agttaagtac
      301 caaatactca tcatttgagc attaaatgct ttaggtgcta acttaacagc agcactatta
      361 cccgttggtg ataagcaaag ttcaccgata acacagataa tgtacgataa aataacccag
      421 ttaactgaga agtttgatga acctgatgca taacctacaa taccaattag tatgtatgaa
      481 gcacctgcta agaacgtacc aattgcaaat ttcactggca ggctaggttg tttagttcca
      541 agcttttgcc ataaaagtga aataattgga gctaataata aaataaataa tgggttaatt
      601 gattggaaga tcgcttcacc aaagtttgtt ttccaaccaa ataagtttaa tttcatatct
      661 gaatgttcaa ttccataaat gtttaataca ttagaccctt gttcttgaat agcccagaaa
      721 accattccaa gaataaataa tggaataaat gctttaacac gagaacgttc agtatcagtg
      781 acatctttac ttctaataat taaagtgaag taaatgattg gtaatgcaat acctaatact
      841 aaaacagtat tactaactaa gttaaatgat aatgagttag ttaatgcacc aataacgata
      901 attaatacaa ttgctaagat aacacttccg ataataagac catacttttt cttttcagct
      961 ggtgtcaatg gattagtagg tttcatacca acgctaccta agtttttgcg gttgaaaagt
     1021 acataccata ctaaacctaa tgccatacca acagctgcaa tcaagaatcc gccgtgaaag
     1081 tttttaacat taacaaagtg ttgcaaaata ataggtgata ataatgcacc catattaact
     1141 gacatataga aaataacaaa acctgcatcc atacgtctat cattttcagg atataaacgg
     1201 ccaacgatat ttgaaatgtt tggcttcatt aaacctgaac caataatgat gaagaacatt
     1261 gatgtgaata agccgattaa tgcaaatggt aagcttaaac aaatatgtcc gataataata
     1321 aagactgcac ctaataaagt agcgcctcta gtacctgtaa ttctgtcagc aatccatccg
     1381 cctggtattg atgtcatata gattaatgaa ccataaactg acataattga catagctgtt
     1441 gttttatcaa ttccaaggcc attatctgtt acggcaaagt acatgtagaa aatgagtagg
     1501 gcacgcatgc cataataact aaacctttcc cagaactcta caaagaagag tacgcctagc
     1561 cctcgaggat gcccgaaaaa tcctgtttga ggtatgtctt gaatttgatt tccatgggag
     1621 ttttgttgtg tcatttatac atcccatcct ttcttcccct aatacaatag tttaagtaaa
     1681 tttgattgtg ccttatcaac taaatatttc acaaatcaca aaaaaataaa aatattgaga
     1741 atattctgtt attaagcggg cacaattaat tagagataat ataaaaatat acttattgac
     1801 gttaaagtgc aataggtttc tataaaaatg ttgtttaatt cgtgtactaa tattcttttg
     1861 gtgtactttg gtataaaatg ttattttaat gtaacctatt atgtatgcta aatgaaaaac
     1921 acaccaataa tgtaggaaaa cctaattatt ggtgtgttta ttaaatgata catttaacga
     1981 ttatctattt tttcgggata taagtcatga ttcattaaac gatgttcagc catttgttcg
     2041 tatttagaat ttggacgtcc atagtttgta taagggtcaa tagaaattcc accacgtggt
     2101 gtgaacttgc cccaaacttc aatataatgt ggatccataa gctctatcaa gtcattcata
     2161 attatattca tacaatcttc atggaaatca ccgtgatttc tgaaactaaa taagtataat
     2221 ttcaaagatt ttgattcaac cattttaaca tttggaatat atgaaatata gatagttgca
     2281 aaatctggtt gcccagtaat cggacataat gatgtgaatt ctggacagtt gaattttacg
     2341 aaatagtcac gaccttgatg cttattatca aacgattcta atacatcagg acgatagtca
     2401 aaattgtacg tattgtcttg atttcctaat aaagttatat cttgtaattc atcttgttga
     2461 cggccatgtg ccataaaaag cgctccttta aatttatttt tttattactt tggcgtctcg
     2521 gcgtgctttt tcaaacatgt aataacttgc accgataata acgacgtaac ctaatgttgc
     2581 atagaaatct ggagattctc caaatagaat aaatccaagt attgctgtga aaattataga
     2641 tgcatatgtg aaaatagaaa tatctttcgc agcagcaaaa ctatatgcca atgtaacgcc
     2701 aatttgaccc acagcggcag ctaagccagc ccctaatagg taaagtattt gcgtttgact
     2761 cattggttca tatgtatatg cagtaaatgg tattaatacg atgactgaaa ataatgagaa
     2821 gtaaaataca atggtgtatg gtgcttctct agtactaagt gcgcgtacac aagtatatgc
     2881 cgatgcagca aaaatacctg aaaatagtcc agctagtgat ggaatcatag atgatgaaaa
     2941 ttcaggtttc actattaaaa gcatacctaa aatagcaatt atcattgctg ttatttgata
     3001 cttccttacc ttttcatgta agaaaacaat acttaataaa atcgtccaga aaggattgag
     3061 tttcattagt gaatcagcat cgctaagtac catatggtca atggcgtaaa tatttaataa
     3121 tacacctatg agtccaagtg ttgatcgtgt taataataat ggttggcttg agagtttacc
     3181 aaacattggt tgatgatatt tatatataaa aaataatgga ataaacattg ctactaagtt
     3241 tcgtgctaat gatttttgaa aaacaggaag atcacctgca agtctgaaaa acactgacat
     3301 aaaactgaaa ccaatagccg aaattaaaat ggcaatgata ccttttactt taggattcaa
     3361 ttttatcgcc tcttttatat aaaattaacg tatttatatt agcataaaac aacatgttgt
     3421 gcataaatag ttgaaattta ctataaaaag actataatag attttaccga acaaatgttc
     3481 tgtgtttatt tgtcggaata atagggcatt acacttttat gaatgtttgt gttattacat
     3541 aaaacaaata tcaattcagt atcaagctaa taagcttttt cttgatttct gttgatacaa
     3601 ttgagattga cacagatttt aaaaaatcaa gtgatatcta ctgaaaaaat ttttttaatt
     3661 tgttcaagtt tttctaatta agtattggtg cctagttgga acgttttacg aacattcgat
     3721 tagaaaatga cactttaaat catagtgtgt cttatgtata atgaaacaca taatatagtg
     3781 ttggttaaac gaaaaagaca caatatcttg tgttttgtat gcaaatgcat tatttatgaa
     3841 gaaattacat ttaaaagtaa tttaa
//
LOCUS       DAPIDM010000184         3762 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000184 DAPIDM010000000
VERSION     DAPIDM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.186"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(451..1272)
                     /locus_tag="RJE42_002518"
     CDS             complement(451..1272)
                     /locus_tag="RJE42_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV5877909.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGSKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGNVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(1290..1985)
                     /locus_tag="RJE42_002519"
     CDS             complement(1290..1985)
                     /locus_tag="RJE42_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV5877910.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFTFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAASFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(1978..3003)
                     /locus_tag="RJE42_002520"
     CDS             complement(1978..3003)
                     /locus_tag="RJE42_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDV5877911.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(3092..3195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3253..3549)
                     /locus_tag="RJE42_002521"
     CDS             complement(3253..3549)
                     /locus_tag="RJE42_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV5877912.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFQSVTDLLENLK"
     gene            complement(3542..>3762)
                     /locus_tag="RJE42_002522"
     CDS             complement(3542..>3762)
                     /locus_tag="RJE42_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topiosmerase"
                     /protein_id="HDV5877913.1"
                     /translation="ADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHV
                     LSKHGFTCKKETPFLPNINNERLVLVNE"
BASE COUNT         1158 a          716 c          532 g         1356 t
ORIGIN      
        1 gtcccacccc aacttccaca ttattgtttg ctggctttcc gcctgctact atgttggggc
       61 cccgcaatta ttgtatactg gctttccatc agcttctatg ttgggcccaa ccccaacttg
      121 cactgtttat aaaatttctt ttcgaaatcc tctgtgttgg ggacccgcaa ttattgtaaa
      181 aaggttgtta caatcgtatt ttcattcaat caacaactgc ttatataaca ttgtaaatct
      241 atggacattg attgaaatca cagaagcata taggccttca ctttatatat aatgacttga
      301 taatcattta tatatagtta ttcatctctt atcatattaa atcttccaca tttcatattg
      361 attctaattt aaaaaactcc catcaaagtt tgcatttttt caatgtctac tttgatggga
      421 gcatatcagt caatgagctc taactaaaaa ttatttagca ggaataactg caccgttgta
      481 tttttcatta atgaagtctt gaatatcttt agattgtaat acttcaatta atgctttgat
      541 tttcttatca tcttgatgtc cttctttaac agcaattaag tttgcataag gattatcttt
      601 cgcactttct acagcaatag aatctttttt aggatttagt ttttgttcga ttgcaaagtt
      661 tgaattaatg ataacagcat ccgcgtcttc attttgataa attttaggta agaattctgc
      721 tgattgttta ttattaaact taatatcttt tttattctct gtaatatcac taaacttagc
      781 atcttcaatt tttacgcctt ttttgatctt aattaaacct gcatcaacga agaattttaa
      841 gaagcgtcct tgttcagctg gattattaga cacataaact gttgcacctt ttggtaattc
      901 ttttaaactt ttatactttt tagagtatac agccataggt tctaagtgaa cattaccggc
      961 acttacgatt ttgtaacctt tatccttttt ctctgtgttt aaatatggtg tatgttggaa
     1021 atagtttgcg tcaatttcac ctttgtctag taatttatta ggcgtagtgt aatcgttaat
     1081 tgttttaata tctagttcat aacctttttt ctctaataat ggttttgctt tttctaaaat
     1141 ttcagcatgt ggtgctggtg aagcacctac tgttattttc ttgtcgtcac tgccactttt
     1201 gcttccatta ccgcaagctg ctaatacaac tgcaaatgtt aatactaaaa taagaccaaa
     1261 taattttttc ataaaatgaa acccccaatt tatcgtttat caagtttatt tgtaagccaa
     1321 tccccaatga attggattat aaatacaata attaaaataa aaactgttga tactaaaatg
     1381 acatcatttt gatttcgagt gaaacctgtt aagtatgcta aatttcctaa accaccggca
     1441 ccaattacac ctgcaactgc cgttgaacca actaaagcga ttgctgtaac tgtaatgcca
     1501 gacactagcg ctggcatggc ttcaggtaag aggactttac gaattactgt ccaagtatta
     1561 gcccccattg accaggccgc ttcgatgaca cctttatcaa tttctttaaa agcaatttct
     1621 acgagccttg cataaaacga cgctgcgcca atgatcaagg ctggtaacgc acctgtcgga
     1681 ccacttatcg ttccaagtat caaacttgta aatggtatta ataataaaat taaaataata
     1741 aatggtatcg ctctaaataa gttaacaatg aaagaaacga tagaataaaa taaccttgca
     1801 ccgatagact tacctttagc agacaagaat aataacacac ctaaaataag accgagtata
     1861 aatgtaaata tagttgagac gactgtcatg tatagtgttt cgactattgc agtccaaact
     1921 tctggccact gaatattagg cattgtaatc atttcattta taatttcact aaatgattta
     1981 cccatgtctt aacacctcca ttttaacttg tcgctcaatt aactcttttt cgaattttcc
     2041 gaaatctaca cttgaaatat atggaatatg cagaactaaa aagccgactg ttccattttt
     2101 tgtattttta atatttgctt ctaaaatatt aattttaata tcataggcag ttgatagact
     2161 cgatacaata ggctcggttg ttgttgaacc agcgaaaact aatctaacga tataagcatc
     2221 tttttctaat ggctctaatt ctgttaaaga tgtttcgaaa tcatcgttta aatcttcttt
     2281 cacaaatcgt tttgtcacag tgtgttgcgg attttcaaaa acctgtgtca ccggtccatg
     2341 ttctatcact ttaccacttt ccataactgc aacttcatca caaatacgac gaatgacatg
     2401 catttcatgc gtaattagta caattgttaa attttgttgt tctctaattt ttagtagtag
     2461 atctaaaatt tcatctgttg tttgcggatc aagtgcactt gttgcctcat cacaaagcaa
     2521 gacagttgga tcattagcta acgctcgtgc aatcccaaca cgttgctttt gtccacctga
     2581 taactctgat ggataagcct tttctctacc ttttaaaccg acgagttcga caagttctaa
     2641 tgctttttgc tttgctctcc ttctagggac acctgcaatt tcaagcggaa acataatatt
     2701 ttttaacaca gtccttgacc ataacaaatt aaaatgttgg aaaatcatac ttactttttg
     2761 tctttttgct cttaatccat ttttggacaa ttggcctata tggtctccat ctataataac
     2821 ttcacctgat gtaggcgctt ctagatgatt aaacattcga atcaaagtac tttttcctgc
     2881 tccagaaaaa ccaatgacgc cataaatcga tcctgctcga atcgataaat taacgtgatc
     2941 tacagcaagg acttctttat ttttagtccg atattcttta acaacttctt ttaattcaat
     3001 cacgttgatt cctccctgtg ttgcttaata aaataaaaaa tgctttctca atatcgatag
     3061 aaaaattgag aaagcaatag tagtattgtt tttctcatct tcaaaagtta aaactttatg
     3121 tgaattggca ccatttctat ataagacggt tgccgggctt cgtagggcac atccctccac
     3181 cactctcgat aagagtttac gcatcattta atttgtatta atcctaacac cttagtaaaa
     3241 tttcgtcaat aactatttta aattttctaa caaatcagtc accgattgaa atgcataaat
     3301 tcgttttact tctttatctt tattcatcaa caataaaatc ggcgtagaca tgatttgcat
     3361 atctttacaa aactgaggat aaaagtttaa atctattttc aataatggca actgcaatat
     3421 ttcattagca atgtctaaca ttctttctga aaccttacaa gtaccacaag ttggtgtata
     3481 accaaagatt aaatgtttgt cttcctcata aaatgtagtt acatctttga tgtctaatga
     3541 attattcatt tactaaaact aacctttcat tatttatatt cggtaagaaa ggtgtttctt
     3601 tcttacaagt aaagccatgt tttgaaagta catgcgccaa atattgtttg gggcaattcg
     3661 caacttggca gtaagtttta tcaataaata tatgttcact ttcactcaaa taacgtttaa
     3721 accaatttct aattcgatct ccttcgtcat cagaatcggc ta
//
LOCUS       DAPIDM010000185         3696 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000185 DAPIDM010000000
VERSION     DAPIDM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.187"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            56..283
                     /locus_tag="RJE42_002523"
     CDS             56..283
                     /locus_tag="RJE42_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877914.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(600..1547)
                     /locus_tag="RJE42_002524"
     CDS             complement(600..1547)
                     /locus_tag="RJE42_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDV5877915.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMLKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINNLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLVNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     VFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPVIWKG
                     LLFIEMLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            1795..1983
                     /locus_tag="RJE42_002525"
     CDS             1795..1983
                     /locus_tag="RJE42_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV5877916.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANGIFSNEN"
     gene            complement(2455..2528)
                     /locus_tag="RJE42_002526"
     tRNA            complement(2455..2528)
                     /locus_tag="RJE42_002526"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Arg,seq:tct)
     gene            2892..3026
                     /locus_tag="RJE42_002527"
     CDS             2892..3026
                     /locus_tag="RJE42_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDV5877917.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            3083..3217
                     /locus_tag="RJE42_002528"
     CDS             3083..3217
                     /locus_tag="RJE42_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDV5877918.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            3341..>3696
                     /locus_tag="RJE42_002529"
     CDS             3341..>3696
                     /locus_tag="RJE42_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HDV5877919.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFYYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKR"
BASE COUNT         1373 a          478 c          651 g         1194 t
ORIGIN      
        1 tgtaacacat gatacatatg gttgtgaaaa tagcatatat aaaggatggc tataaatgac
       61 tcatttgaca aaggttttag atacactaac tggaatatgc gtagtattat tatttagtaa
      121 atattttgtg gcgtatgcaa atatggtatt tgattggaat ttaagatggt atttgctaga
      181 aaacatacca catttgccaa ttatattatt tattctgatg tttattttcg gagttccttc
      241 tgaaatgata aaagataggc aaaggaaaaa taacggcgtt taatttatcg atattttggg
      301 gtgataaaac ttgtcaacta ttacaaagac taaaataaaa atctttatta attattttat
      361 tggtatatta gctttggcgt ttgctgctta tatattattt aaataaagaa tagagtgaaa
      421 cgtgtttatg aattagacgt tctagtatag ttactacagc tttagtcaga aggtatcatt
      481 gttgttcata cattagatca tattaaatca aagaggtatt gatacacacc aaaaaaggag
      541 gcaagattac ctgcctcttt tttagttatt aaatatgcgt gtaattcttg gtattagtgt
      601 tactgagacg cattacgaag aaatttatct tgattttcct tttttaaaaa gaagtgaaga
      661 tatccaataa agactctaag tactattaca gttgctaata acatttcgat aaacaaaagg
      721 cctttccaaa tgactggaaa cataagtaca ggcaaactgt tctttaaagc agttgcagag
      781 attactaacg ggaatgtgaa agctgaaaat acgggcgaaa aaggttcttt tagtaattta
      841 ggcaattgta taatgatata aaaataaaat atttgagcta atagtaaaaa tacaattacg
      901 ataaaagcat ttgccttagg aaaggctata acatatgctg cagcgactaa agaaaatggt
      961 gcacaaattg tcgatgtgtt cggtttgatt gacgtctgca atggaaatgc ttttaatcgc
     1021 ttgaaaacta taggtaagac aatacaagta gctacaaagc catatattac tgttaattga
     1081 cctatgaaaa aatatccgct aacgggtgcc gtcaatcctg cgatggcaat accaataaaa
     1141 agtacagtcc acgaaggata aacattcaca agtgaaaaat cttttaaata tttaattgaa
     1201 aaaataatca tatgtgtcat aattcccaca aggcataaaa tccaaatagg cgttattaaa
     1261 ttattgataa atgttatatt actaaaaaat gtatttaaat aagtagtacc taaaaagcca
     1321 gacatgaaaa atgttgtgaa cactgatgaa actagagggc tatttaattg ttctttcaca
     1381 ttattaaaat atttgagcat agtacataaa aggtgaatcc aaatcaagaa agcaaagatt
     1441 ccgcaaacag cgtttaaagt aagagataaa tcttttaata gattacccag gcctaataat
     1501 cctaagacta gtcctgacgt tactagaggt gctttttgaa gtctcatgat ttacaacctt
     1561 ccttatgtga ttttcttcac taattatatc atgatagcta tgtcctatta ataagaagag
     1621 tgtgtactat tacgttttta gagtatgtat attggattga tagacacata attgacattt
     1681 aaatctcaat attaatgata tttgaggtat gctttgctac ctaaatatgg aatatgtgga
     1741 aaagtattta tttaatttaa atgatgattg aagatttaca agggggtgta caaattgaga
     1801 aatcaaattc aaaaactatt agacagtgat ttgagcagtt tacatatatc gaaacaaaca
     1861 ggagtgccac aaagcacaat acacagaatg agaaaaaatg aaagatcatt agacaatatg
     1921 tcattgaaaa acgctgaact actttataaa tttgccaatg gtatatttag taatgaaaat
     1981 taagaagaat aattagtgag ttaataatta gaaaaccacg tctcgatggg cgtggttatt
     2041 ttttagatgt atataattat aattatagga tttcgggtcc ctgaaaagtc cctaaaattt
     2101 tgaatagtat caaaatctat aggaataata taataataaa aatctctacg catgcaaatg
     2161 gtgagtgtta aaaattttgg tgtagttttg gtgtagttca ggtgcagtat agatgaaatt
     2221 tgagcgtaaa attacttaat taaaaatgtg taaacatgta tcaaccgcgt gtcaattgtg
     2281 tgtcaaaaaa ttctataacg aataagcatg catgttttaa ttactgtatt ttcggggctt
     2341 atgcggggga cgttttggtg acgcatcata ctatattact gaaattcaaa acaaaaagag
     2401 ccccgtaatc acggaactct tttgtttggt aatgcgtata aaaataccta tgaacgtcct
     2461 gggagggatt cgaacccccg accgatggct tagaaggcca ttgctctatc cagctgagct
     2521 accaggacac gtttaacaac acaagaatta ttatatctaa atgaacttaa attagcaata
     2581 ccttgtaaat aaaaaatgtt tatatttttc actattatag agctatttat ctaaaaaggt
     2641 tcaataagac ttaaatacga attcaggcaa cttaattgtg ttaaatacag ttttgaatgc
     2701 ctaactgtat ttcttttctc tttaaaatac agttaagtac attataagat gttgtgcgga
     2761 taaacaaact aattgcatca aatttatttt aaaataacaa caacaaaacg ttaagcgaat
     2821 aacatttcag tgatttaaaa gctacgcacg tttttgttat cttcaaattt aaattttaag
     2881 gagtgttttc aatggaaggt ttatttaacg caattaaaga taccgtaact gcagcaatta
     2941 ataatgacgg cgcaaaatta ggcacaagca ttgtgagcat cgttgaaaat ggcgtaggtt
     3001 tactaggtaa attattcgga ttctaatttc aatatgttat gtaagtaata agtattattt
     3061 caaaggtgag ggagagattt aaatgactgg actagcagaa gcaatcgcaa atactgtgca
     3121 agctgcacaa caacatgata gtgtgaaatt aggcacaagt atcgtagaca tcgttgctaa
     3181 cggtgtaggt ttactaggta agttattcgg attctaatat aataactaat attctttaaa
     3241 ataaactggg tgagcatact ttaatgttat gcactcagtt tattttattt gcagaaattt
     3301 gagcctctgt taagatttag atacatagac aatataggag atggggaaat tgggatataa
     3361 aaatattttg atagactttg atgatacaat tgttgatttt tatgatgcag aagaatgggc
     3421 attttactat atggcgaatg tttttaatca taaagcaaca aaggatgatt ttttaacatt
     3481 taaaaaaatc aatcaccaac attgggaagc ttttcaacaa aataaattaa cgaagtctga
     3541 agtattatca gaacgatttg tgaattactt caaacatcat caaatggaag ttgatgggca
     3601 tcgtgcagat gtgttattta gaaatggatt agcagaagct aaagttaaat actttgatca
     3661 aacattagaa acaattgtcg agttatcgaa aagaca
//
LOCUS       DAPIDM010000186         3538 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000186 DAPIDM010000000
VERSION     DAPIDM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.188"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            134..775
                     /locus_tag="RJE42_002530"
     CDS             134..775
                     /locus_tag="RJE42_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDV5877920.1"
                     /translation="MEIMLCESKVINKNPKYRIIKYESEYLMIDLASNRIVFFFPFIN
                     WLIPKTYIKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLGIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
     gene            822..1451
                     /locus_tag="RJE42_002531"
                     /pseudo
     CDS             822..1451
                     /locus_tag="RJE42_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            1905..2534
                     /locus_tag="RJE42_002532"
     CDS             1905..2534
                     /locus_tag="RJE42_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDV5877921.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYVHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            2658..3299
                     /locus_tag="RJE42_002533"
     CDS             2658..3299
                     /locus_tag="RJE42_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDV5877922.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLISTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTTKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLLFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKSNGIEKF"
     gene            3336..>3538
                     /locus_tag="RJE42_002534"
     CDS             3336..>3538
                     /locus_tag="RJE42_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDV5877923.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWFI
                     PKKYVKISREEFESLNIVKPVKN"
BASE COUNT         1290 a          359 c          556 g         1333 t
ORIGIN      
        1 taaatggtgg taataaattg agattaatga tatgcaaatg acaatgaata aaatttttaa
       61 tgatagtaag ttgataatat tttaatgtct tttgaagtat cgaaaaatgt attacagata
      121 aaatcaaatg gaagtggaga taatgctttg cgaatctaaa gttattaata aaaatcctaa
      181 atatcgaata atcaaatacg aaagtgaata cttaatgatt gatttagcaa gtaataggat
      241 tgttttcttc tttccattta ttaactggct cataccgaaa acatatatca aaatcactaa
      301 gaatgattat gaaaaattaa atattgtcaa accagttaaa aataaatcga taggatggac
      361 tatattcgcg ggtattgtgt tacttggtgg tacagtaaga agaaatactt atttatttga
      421 ttttcaatta gaagaactaa ttgtttggag cagctgtttc attgggtttt tagggattat
      481 atttttttat tgttatctaa ataagaaatt aacattaaat atttataatg aaagtaaaaa
      541 taatgaactt aaattaagat tattaccctc ctttaaaaat atttgtttca caatttttta
      601 ttacctattt actggtttca tgtcttatgg ggcattttac ttgttggtgt ttgaaaatgt
      661 acaaaattta atcttatatg tttcttggct tttcatgact atgctattta tgtttatgaa
      721 catgcattca attatagata aaaaagtaca tatattctta aagtctaata aatagttaca
      781 aatttagtta gttttcaatt gtaaattagg ggtggtaaac agtgctttgt gaatctaaac
      841 aaatttataa aaatcctaaa tatcgagtta ttagatataa taatcaatat ttcatggtcg
      901 atttagtaag tacttggatt acttattttt ttcctatgat taattggttt ttgcccaaaa
      961 agtacgcaaa aattagcgaa aatgaatttg aaaggttaaa tattgtcgaa cctgttaaaa
     1021 acaatgtttt ttggccggtt gcaggaagtt cagttctatt cggaattata ttgagaaagt
     1081 acggtaattt ctttaatgtt cagtttgaaa aacaactagc aatcactgta ttttttatca
     1141 tgttaatagg gatgttaatt ttttatttct atctaaataa aaaattaaca ttaaaaattt
     1201 ttaataccaa cgtggttaat aagaatagag ttgtattaat accgactttc aaacaagggt
     1261 tgttattagt ttttgcgtac atttttttag gaagcgcttc aatatttact ttaaccattc
     1321 ttttgacgac agagtcataa aatataataa tatttttaac ttgggttatt atcacgatgt
     1381 ttttcttttt agtgaatatg gcttcgatag gtaatacaaa tattcatgtt attttaagaa
     1441 taaacgagta gtaaaaatga tcgaaattgg tagtatcgca tatttaaacg gtggttcaaa
     1501 aaaatataat catatcttaa atcaggaaaa tagatagtaa tgtttgaata taccaagtga
     1561 aaatgcccat tgtgattgat aaaggaaaaa actgttttaa tttaaaaatg aaatatagag
     1621 cgtgttattt tgtatgatag ctcttataac attggcgttt tttaatccaa taggatggac
     1681 ggttttagct ggttttgttg cggaatctgt ttagacaatt cttataaatt ggacttttca
     1741 tactaataaa tttggattaa gagataaaac taattggata agacattaaa tagacgctgt
     1801 ttaaaaatac cacagaaaaa gctgtagata gtgttggaac gatagttggt gaaggtacaa
     1861 aaagtattgg ggatcattta aaactaatga aacgggagtg gtaaatgttg ctttgcgatg
     1921 ttagagtcat ttataaaaat ccgaaataca aagtcattca acataacggt gaatacttat
     1981 tagtcgattt agtaagcact tggttcgtgt actttttccc tttcattaat tggttcattc
     2041 caaaaaagta cgcgataatt agcgaagaag aatttgaaaa tttaaatgtt gttaaaccaa
     2101 ataaaaataa tgttttctgg tcagttatag gaagttcggt tttgtttgga gttactttaa
     2161 ggaaatacgt acatgttttt gatgttcaat tagataagct agttgtaatg atattgtgtg
     2221 ctctcgcttt aatttgtgtt atagtttttt attttaactt aaatagaaag cttaagttaa
     2281 aagtgtttga tacaaatatt gaaaaaaata agagagttat attaatacca acgtttaaac
     2341 ttggctgttt tttagttttc ggatatattt tcgctggaag tttttcaata ttttcactaa
     2401 ttgcccttat gacaatcgaa cctcaaaata taataatatt tatttattgg attatgatga
     2461 caatgctttt ctttttgtta aatatgactt cgataggtaa tgaaaaagtt cgcgttataa
     2521 tgaaaaataa ttgattacat ttaaaatatt ctaaatgttg tcgacacaat ccttttaaga
     2581 cgctagtaga atttaaatga cttctaatgt atatgaaagt gtatcaatat aaaaccaatt
     2641 gaaaagaagt ggagacattg ctttgtgaaa ctgaaaatat taataagaat cccaaatata
     2701 gaattatcaa atacaaagat gaatatttga tgattgattt aataagtaca tggttagcac
     2761 tctttttccc aatgattaat tggctgattc caaaaaagta cgtcaaaatc agcgaaaaag
     2821 attttgaaac tttaaacatt gtgaagacaa ctaaaatcaa ttctttttgg ccagtggcag
     2881 gaagtacggt cttatttggt gttatgttaa gaaggtattc ccatttattt atcgttaaat
     2941 atgaatatag tatagtaatt ttaatttgtt gcatcataat actaggtatt tttctgttat
     3001 ttttatattt aaatcaaaag ttaaagttac aaatctataa tgaaaacaaa aataaaagca
     3061 ataagataat catatttccc actttaaaga gtctttgttt atcaatagtt ttatatattt
     3121 atttaggtgg tggttcattc tttactattt atatgttatt gacgattgaa gttcaaaata
     3181 taatattatt tataactttg tttgtaatat ttttgttttt cttattttta aatatgtgct
     3241 cactatatga caataaagtt catgtattat ttaaatcaaa tggaattgaa aagttttaat
     3301 aggaataata aacaaaggaa tggtagtgat aagtattgct atgtgaatct aaagttatca
     3361 acaaaaatcc taagtataga gttattaaat atggtgatga atatttaatg attgatttag
     3421 taagtacctg gttaacttta tttcttccta tgattaattg gtttattccc aaaaagtacg
     3481 tgaaaattag tagagaagaa tttgaaagtt taaatattgt caaacctgtt aaaaataa
//
LOCUS       DAPIDM010000187         3401 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000187 DAPIDM010000000
VERSION     DAPIDM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.189"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            383..1342
                     /gene="hyl"
                     /locus_tag="RJE42_002535"
     CDS             383..1342
                     /gene="hyl"
                     /locus_tag="RJE42_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDV5877924.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKILVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLSDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDSGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
     gene            1795..2028
                     /locus_tag="RJE42_002536"
     CDS             1795..2028
                     /locus_tag="RJE42_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877925.1"
                     /translation="MVFKDWEINVVYSGKHEVVTNENSLFVIDEDYDVAIAINYLDNK
                     LKVSHVNYGSEFTIDASNKVLELMIHNPNIDEN"
     gene            2638..2823
                     /locus_tag="RJE42_002537"
     CDS             2638..2823
                     /locus_tag="RJE42_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877926.1"
                     /translation="MNKHINKKTTKHLEDYKKVQQRNSEETNRGCLKSAAMLIVLFLF
                     IIFGIVACSTHVNFFFQ"
     gene            complement(3071..>3401)
                     /gene="scc"
                     /locus_tag="RJE42_002538"
     CDS             complement(3071..>3401)
                     /gene="scc"
                     /locus_tag="RJE42_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complement inhibitor SCIN-C"
                     /protein_id="HDV5877927.1"
                     /translation="VAGTLAVLLSTTAVSTLDGNKADASSKKDYIIQSEFHDKRIAEE
                     LKSLLDQSYVNDLAAGSLNPYYKRMIMMNQYRAKAALKSNNFAKMAEAKVGLENIYKE
                     IDEIINR"
BASE COUNT         1257 a          488 c          480 g         1176 t
ORIGIN      
        1 ttaacagtta attaattgat ttaattctaa gatatttgta tttttatatt gtaatcgatt
       61 acattttaaa gttaaataaa cacccagttt taaagaaatt aatcaataaa attagctttg
      121 tcttttcctt ggttcataaa gagttcattc aactttgact aagcctcgaa attgaaatgc
      181 ttcctttcaa atattaatta aaagttaaaa acatattctt aaaatattca ctcatcatca
      241 ctcaatcatt taacagttgc tacattaaat atactaatat aaacgatgat aaatatttga
      301 tatgtctcaa ctgcattatt ctaaattgac atattgatta ttgtttcctc aaatcgtttt
      361 aaaaaataga aggatgatga aaatgaaaac acgtatagtc agctcagtaa caacaacact
      421 attgctaggt tctatattaa tgaatcctgt cgctaatgcc gcagattctg atattaatat
      481 taaaaccggt actacagata taggaagcaa tactacagta aaaacaggtg atttagtcac
      541 ttatgataaa gaaaatggca tgcacaaaaa agtattttat agttttatcg atgataaaaa
      601 tcacaataaa aaaatattag tcatcagaac gaaaggtacc attgctggtc agtatagagt
      661 ttatagcgaa gaaggcgcta ataaaagcgg tttagcctgg ccttcagcat ttaaggtaca
      721 gttgcaacta tctgataatg aagtagcaca aatttctgat tactacccta gaaattcaat
      781 tgatactaaa gaatatatga gtacattaac ttatggattc aacggtaacg tgactggtga
      841 tgattctgga aaaattggcg gtttgattgg tgcaaatgtt tcaattggtc atacgctcaa
      901 atatgttcaa ccagatttca aaacaatttt agaaagtcca actgataaaa aagtaggttg
      961 gaaagttata tttaacaata tggtgaatca aaattgggga ccatatgaca gagattcttg
     1021 gaacccagta tacggcaatc aacttttcat gaaaacaaga aacggttcaa tgaaagcagc
     1081 agataacttc cttgatccta acaaagcaag ttctctatta tcttcagggt tttcaccaga
     1141 cttcgctaca gttattacta tggatagaaa agcatccaaa caacaaacaa atatagatgt
     1201 aatatacgaa cgagttcgtg atgactacca attgcattgg acttcaacaa attggaaagg
     1261 taccaatact aaagataaat ggacagatcg ttcttcagaa agatataaaa tcgattggga
     1321 aaaagaagaa atgacaaatt aatggttatg tacactcaaa tagtcacata ataatattag
     1381 ccgataacat cagaaatgat gtgcgtcggc tattttagtt gcagtataaa attaaacatc
     1441 atgaatagta atatacctat tatgcttttc agtatatatt cacggctaaa atcatgatta
     1501 cggtactgat ttcttaactt caaacatttg aaattcatgt tgtcaaaccc tttaattaaa
     1561 agttttgaaa ttgttcaaaa cttattgaac acattatgtg ttaatatatt tgcagaatta
     1621 ttttaaaatc gttttaaaac tatactgaaa taagaagata aaaagtataa taagtacata
     1681 taattgaaaa taatttctag aaatctattt atctctaatc tagatattgt aagaagttgt
     1741 atgaataagt taaacttaga ttgaatcagt ttaaatgttc aaggaggatg acaaatggtt
     1801 tttaaagatt gggaaattaa tgttgtttac tcagggaaac atgaagtagt tacaaacgaa
     1861 aatagtttat ttgttattga tgaagattat gatgtcgcaa tcgcaatcaa ttatttagat
     1921 aataaattaa aagttagtca tgttaactat ggctctgaat ttacaattga cgcatctaac
     1981 aaagtacttg agttgatgat tcataatcca aatattgacg agaattaaat attaaaaaac
     2041 atttagaaac ataaaagcat actatatata gtcttttaaa agtataacga acgaaaatta
     2101 gaaattcgga tattcattac actattaaaa tgcaattttt caaagcattt aaaaacaaca
     2161 gttaaggtgg tgaccaaaaa ttaacattgt gtataacatc attgtatata tagcatgcat
     2221 gattaacttc ggactgatta tcgtcccatt gctaactacc aatgacgcag attttgtttg
     2281 gacttcttcg tcattaatat atatgatcct tttaacacta cttatattaa ttgtttatat
     2341 taaaactaaa gatagtttac agctaacaat atttatactt agtgtggcat taatccttct
     2401 atactgtgca cctttactgt tcatttatgt attctaatgt ttcattaata ttttaatacg
     2461 tcagtttaaa aacaaacaaa ttggaaaata aacgaaacaa gttatcatct aatttaacat
     2521 gatactcact taaaatttga aacaacgagc agcctaactt aagctttata tctttatttc
     2581 aagttatgtt ttcaactatt ttaatgcaat ttacatttag attaaggagt aaacatcatg
     2641 aataaacata taaataaaaa gaccacaaag catcttgaag attataaaaa ggttcaacaa
     2701 agaaattcgg aggaaacaaa tagaggttgt ttaaaaagcg ccgccatgct tattgtctta
     2761 tttctattca ttatatttgg catcgtggca tgttcgacac acgttaactt tttcttccaa
     2821 taatatactc attgagtaaa aatcagaacg tttcatacta cgtttaaatt tatctatggg
     2881 agcactataa atatttttta gattgtacaa agcaattttg tgcataatat ttctaactat
     2941 tatatttata tttgtctccc ctattttttg tatacattgc tcatacataa atattaaatt
     3001 gcagaatttt taactgtaca aataaaaaac aggcgaagac aatggtatta ttgtctcagc
     3061 ctgtttttta ttatctattt ataatttcat caatttcttt gtaaatgttt tctaatccaa
     3121 ctttagcttc agccattttt gcgaaattat tactttttag tgctgctttt gctctatatt
     3181 ggttcatcat aatcatacgt ttgtagtatg ggtttaagct tcctgcagct aaatcattta
     3241 cataagattg atcaagtaat gatttcaatt cttcagcaat tcgtttatca tgaaactcac
     3301 tttgaattat ataatctttc ttactacttg catctgcttt gttcccatct aacgttgata
     3361 ctgctgttgt tgataatagt actgctaatg ttcctgctac t
//
LOCUS       DAPIDM010000188         3376 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000188 DAPIDM010000000
VERSION     DAPIDM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.190"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..1010
                     /gene="prfA"
                     /locus_tag="RJE42_002539"
     CDS             <1..1010
                     /gene="prfA"
                     /locus_tag="RJE42_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV5877928.1"
                     /translation="VVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSE
                     TDDKEEVEMLKEESSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAI
                     FAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKEISFSVSGNGAYSKLKFENGAHRV
                     QRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDSA
                     VRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGD
                     RSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGE
                     L"
     gene            997..1833
                     /gene="prmC"
                     /locus_tag="RJE42_002540"
     CDS             997..1833
                     /gene="prmC"
                     /locus_tag="RJE42_002540"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV5877929.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            1917..2963
                     /locus_tag="RJE42_002541"
     CDS             1917..2963
                     /locus_tag="RJE42_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV5877930.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINELKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKDQLKDFTYTLDARVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITDILPNSI
                     THADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVTF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            2960..>3376
                     /locus_tag="RJE42_002542"
     CDS             2960..>3376
                     /locus_tag="RJE42_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDV5877931.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDIVEEHHLAKTTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEVGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
BASE COUNT         1216 a          495 c          672 g          993 t
ORIGIN      
        1 atgttgtaaa tgattcagat aaattacgta aatattctaa agagcaagct gatttacaaa
       61 aaactgtaga tgtttatcgt aactataaag ctaaaaaaga agaattagct gatattgaag
      121 aaatgttaag tgaaactgat gataaagaag aagtagaaat gttaaaagag gaaagtagtg
      181 gtattaaagc tgaacttcca aatcttgaag aagagcttaa aatattattg attcctaaag
      241 atcctaatga tgacaaagac gttattgtag aaataagagc agcagctggt ggtgatgagg
      301 ctgcgatttt tgctggtgat ttaatgcgta tgtattcaaa gtatgctgaa tcacaaggat
      361 tcaaaactga aatagtagaa gcgtctgaaa gtgaccatgg tggttacaaa gaaattagtt
      421 tctcagtttc tggtaatggc gcgtatagta aattgaaatt tgaaaatggt gcgcaccgcg
      481 ttcaacgtgt gcctgaaaca gaatcaggtg gacgtattca tacttcaaca gctacagtag
      541 cagttttacc agaagttgaa gatgtagaaa ttgaaattag aaatgaagat ttaaaaatcg
      601 acacgtatcg ttcaagtggt gcaggtggtc agcacgtaaa cacaactgac tctgcagtac
      661 gtattaccca tttaccaact ggtgtcattg caacatcttc tgagaagtct caaattcaaa
      721 accgtgaaaa agcaatgaaa gtgttaaaag cacgtttata cgatatgaaa gttcaagaag
      781 aacaacaaaa gtatgcgtca caacgtaaat cagcagtcgg tactggtgat cgttcagaac
      841 gtattcgaac ttataattat ccacaaagcc gtgtaacaga ccaccgtata ggtctaacgc
      901 ttcaaaaatt agggcaaatt atggaaggcc atttagaaga aattatagat gcactgactt
      961 tatcagagca gacagataaa ttgaaagaac ttaataatgg tgaattataa agaaaagtta
     1021 gatgaagcaa ttcatttaac tcaacaaaaa gggtttgaac aaacacgagc tgaatggtta
     1081 atgttagatg tatttcaatg gacgcgtacg gactttgtag tccacatgca tgatgatatg
     1141 ccgaaagcga tgattatgaa gttcgactta gcattacaac gtatgttatt aggagagcct
     1201 atacagtata tagttggctt tgcctcattt tatggtagaa catttgatgt aaactcaaat
     1261 tgtttgatac caagacctga aactgaagaa gtaatgttgc atttcttaca acagttagaa
     1321 gatgatgcaa caatcgtaga tatcggaacg ggtagtggtg tacttgcaat tactttgaaa
     1381 tgtgaaaagc cggatttaaa tgttattgct actgatattt cacttgaggc aatgaatatg
     1441 gctcgtaata atgctgagaa gcatcaatca caaatacaat ttttgacagg ggatgcatta
     1501 aagcccttaa ttaaagaagg tatcaagttg aacggcttga tatctaatcc accatatata
     1561 gatgaaaaag atatggttac gatgtctcca acggttacga gattcgaacc acatcaggca
     1621 ttgtttgcag ataaccatgg atatgctatt tatgaatcaa tcattgaaga tttacctcac
     1681 gttatggaaa aaggcagccc agttgttttt gaaattggtt acaatcaagg tgaggcactt
     1741 aaatcaataa ttttaaataa atttcctgac aaaaaaatcg acattattaa agatataaat
     1801 ggccacgatc gaatcgtctc atttaaatgg taattagaag ttatgccttt gctatgatta
     1861 gttaagtgca tagcttttta ctttgtatta tgataaataa gaaaggcgtg attaagttgg
     1921 atactaaaat ttgggatgtt agagaatata atgaagattt acagcaatat cctaaaataa
     1981 atgaattaaa agacattgtt ttaaatggtg gtttaatagg tttaccaact gaaacagttt
     2041 atggacttgc agcaaatgcg acagatgaag aagctgtagc taaaatatat gaagctaaag
     2101 gccgtccatc tgataatccg cttattgttc atatacacag taaagatcaa ttaaaagatt
     2161 ttacatatac tttagatgca cgcgtagaaa agttaatgca ggcattctgg ccgggcccta
     2221 tttcgtttat attgccgtta aagccaggct atctatgtcg aaaagtttct ggagggttat
     2281 catcagttgc tgttagaatg ccaagtcatt ctgtaggtag acaattatta caaatcataa
     2341 atgaacctct agctgctcca agtgctaatt taagtggtag accttcacca acaactttca
     2401 atcatgtata tcaagatttg aatggccgta tagatggtat cgttcaagct gaacaaagtg
     2461 aagaaggatt agaaagtacg gttttagatt gcacatcttt tccttataaa attgcaagac
     2521 ctggttctat aacagcagca atgattacag atatacttcc gaatagtatc actcatgctg
     2581 attataatga tactgaacag ccaattgcac caggtatgaa gtataagcat tactcaccca
     2641 atacaccact tacaattatt acagacattg agagcaaaat tggaaatgac ggtaaagatt
     2701 ggtcttctat agcttttatt gtgccgagta ataaggtgac gtttatacca agtgaagcgc
     2761 aatttattca attatgtcag gatgacaatg atgttaaaca agcaagtcat aatctttatg
     2821 atgtgttaca ttcacttgat gaaaatgaaa atatttcagc ggcgtatata tacggctttg
     2881 agctgaatga taatacagaa gcaattatga atcgcatgtt aaaagctgca ggtaatcaca
     2941 ttattaaagg gtgtgaacta tgaagatttt attcgtttgt acaggtaaca catgtcgtag
     3001 cccattagcg gaaagtattg caaaagaggt tatgccaaat catcaatttg aatcaagagg
     3061 tatattcgct gtgaacaatc aaggtgtttc gaattatgtt gaagacatag ttgaagaaca
     3121 tcatttagct aaaacgacct tatcgcaaca atttactgaa gcagatttga aagcagatat
     3181 tattttgacg atgtcgtatt cgcacaaaga attaatagag gcacactttg gtttgcaaaa
     3241 tcatgttttc acattgcatg aatatgtaaa agaagtagga gaagttatag atccatacgg
     3301 cggaacaaaa gaaatgtatg tacataccta tgaagaactt gtaagtttga ttttaaaatt
     3361 aaaagatatt atttgc
//
LOCUS       DAPIDM010000189         3336 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000189 DAPIDM010000000
VERSION     DAPIDM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.191"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..404
                     /locus_tag="RJE42_002543"
     CDS             <1..404
                     /locus_tag="RJE42_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877932.1"
                     /translation="HTKTVDLSNLEINDIKLNEKFDKKGYEVNRKIKFDRFKFYNSKD
                     HPDLTVKVREKDNIVKGIILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDF
                     SSVKYIDRDNHLKLNLLYQDLEIKRIEFFSK"
     gene            464..1021
                     /locus_tag="RJE42_002544"
     CDS             464..1021
                     /locus_tag="RJE42_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDV5877933.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEE
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            1018..1860
                     /locus_tag="RJE42_002545"
     CDS             1018..1860
                     /locus_tag="RJE42_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDV5877934.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLDISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSPTYISKSNIKNSNIKVVIGMLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFINGKVGKSDSVLEFYTIDGN
                     IKVE"
     gene            complement(1917..2957)
                     /locus_tag="RJE42_002546"
     CDS             complement(1917..2957)
                     /locus_tag="RJE42_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDV5877935.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVKEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
BASE COUNT         1162 a          428 c          581 g         1165 t
ORIGIN      
        1 gtcatacgaa aactgtggat ttgtctaatc ttgaaataaa cgatattaaa ttaaatgaga
       61 agtttgataa aaaaggctat gaagtaaata gaaaaattaa gtttgatcgt tttaaatttt
      121 ataacagtaa agatcaccct gaccttaccg ttaaagtgag agaaaaggat aacatcgtta
      181 aggggataat attagtaaga gatgaaaaga tacatactaa ttttgatgga ggaattggtt
      241 cgccgataaa taacgcgatt gaaaatcttg gattcggata taaaagaaca aaagttggca
      301 atgatttctc atcggtaaag tatattgata gagataacca tttaaaatta aacttacttt
      361 atcaagattt agaaattaaa cgtattgaat tttttagtaa atagctttag gtcttaaagt
      421 tataaaaaac gaatgaataa ttttattggg atgagtgacc tatatgaaca agattactta
      481 tttaaatgaa ttagaaacag cacttgatgc gctacctagg catttaagag atcaaaaaat
      541 gtatgaatat gaacgttatt tttatgagca agaacttaat ggcgttgatg aagaggaaat
      601 tttaaagaag ttaaaagacc cacaagatgt tgcagctgaa acaaaagcta gaagtgttat
      661 tgattatgct gaatctaaac caacatttga aaatatttca agagctgttg ctgcttcatt
      721 aagtttaggc attctatcta tttttgtcat ccttatacca gtatctatag ttggattatt
      781 tgtattagca ttatttttaa tatcactttt gctgctgttt tgtccaatta ttttattagc
      841 atcagcaata tccagaggaa ttgtagactc aattagtaat gtattttttg ccatatcata
      901 ttcaggatta ggattagtat ttatcattgt catatttaag attttagaat acatttatcg
      961 tttaatctta aaatatttac tttggtatat taaaactgtc aaaggaagcg ttagaaaatg
     1021 aagaaattct tttttattgg gcttttagtg tttgttgtct tttttacagc agcaaccatt
     1081 atttggttca gctatgataa aaacaaatat ggtactaaac aatatgataa aacattcaaa
     1141 gacgatgctt ttgacaatgt atctataaat ttggatagta cagaacttcg tataaagcgg
     1201 gggaatcaat ttagagttaa atatgatggt gacaatgata tattaattaa tatagtagat
     1261 aagacgttga agattagtga taaaaggtct aagacaagag gatatgcaat tgatatgaat
     1321 ccttttcatg agaataagaa aacgttaacg attgaaatgc ctgataaaat gattaaacgt
     1381 ttagatatat cgtctggagc aggaagtgtt agaatcagtg atgttgattt agagaacaca
     1441 agtattcaaa gcattaacgg tgaagtagtt attaaaaact caaatcttga cgcactagat
     1501 tcaaagacaa ataatagtcc tacttatatt agtaaaagta acattaaaaa tagtaatatt
     1561 aaagttgtta ttggtatgct acaaatcgat aagagtcaaa ttaaacaatc catattttta
     1621 aacgatcatg gtgacattga atttaaaaac atgccatcaa aagtagatgc aaaagcttct
     1681 actaaacaag gagatattcg ttttaagtat gatagtaaac ctgaagatac tatactgaag
     1741 ctaaatccgg gaacgggtga tagcgtagtt aaaaataaaa cgtttattaa tggtaaagtt
     1801 gggaaaagcg atagtgtttt agaattttat acgattgatg gtaacatcaa agttgaataa
     1861 ataaaggatg taagcaccga tattaggaag cataatttct ctaatatcgg tgttatttat
     1921 ttgttggcaa aagttaagtc ggtatctata ttgccagtaa agtgagtgat attaaggtct
     1981 tgaccatcta accatgattt gaaatctatt atttctggtg gcgcattttc tcccaatgta
     2041 aaatatgcag ttaatgtttc agggtgatac attgatgtat ggatggtgcc agaccagctt
     2101 ttgaatagtt tactgtaaat ttcatactga ggattgttga ataacttaaa tgctgtagtc
     2161 atatctaaat tatcattagt ttgtgaaatg gtacgcgcta gtctttcttt agattctttt
     2221 gtataattgc gattttcatg tgttaatatt tcaaaatgat ttgtacatat attatcataa
     2281 cgaacatcta ttgatctcgg cgtcacttca acaattgcat ggttcaatga tttgtccatc
     2341 agtatgtagc taaatgagct tctgtgtggt atttctttca ataattggat tgcttctttt
     2401 acatttcggc aattttcaag aattagacga ccaatcatat aacatacaaa accatttgct
     2461 ggtttcttcc ggtgcataaa gttatagccc atagttaatc ctgactcatt cataccatcc
     2521 attcttccag ttacccttga tacaggacca atttgagcta aaccgctatc tgtaggttga
     2581 tatagtaagt agcgaccatc ataagttgca gggtggtaat cgtaatttct aaccatgaag
     2641 tctttgcctt gaaagaccgt gcaaccactt tcttttaaat cggtaaaacg ataatgtcca
     2701 aagtttaaaa taatttggcg tgttggcatt ttgagtatac tttgtagtcc cattaattct
     2761 tcccatattt gaggtgcgta agtttggaat atttgataag tttcatttac atctatatcg
     2821 aaacgtggga cacgtttttt ccattctttt tctcgatttt ttagaagagg tgtttgttga
     2881 agccatttac cagttttaac acctaactcg aaatgtgaac ctctaaaagt cataatatct
     2941 gatgtcactt gttgcatatc atcggcccct ttctttttag ttgtaatata ttgtaaataa
     3001 atagtaatcg tatgtatatt gaatgtcatg ttaaataaag ttatatttta ctaaatgaaa
     3061 tataaaattg tttgaggtga tttctcggtg tataagactt atcaatcagt taaaacatat
     3121 ttttatagat ggtggggata ttaagttaaa aacttaaaat catcttatca taaataacaa
     3181 tcttaagtta gcattcacga taatagtcat tgttaacatt agcatataag gtcatgtcac
     3241 gttgaaacag aggttcctcg gcatttttga aaataatcat tagcttgctc gctatataat
     3301 ttgatgaata tatttcgtaa aagtgggtat ttattt
//
LOCUS       DAPIDM010000190         3290 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000190 DAPIDM010000000
VERSION     DAPIDM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.192"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(56..202)
                     /locus_tag="RJE42_002547"
     CDS             complement(56..202)
                     /locus_tag="RJE42_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877936.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVENVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(242..424)
                     /locus_tag="RJE42_002548"
     CDS             complement(242..424)
                     /locus_tag="RJE42_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV5877937.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(524..946)
                     /locus_tag="RJE42_002549"
     CDS             complement(524..946)
                     /locus_tag="RJE42_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDV5877938.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            1031..2305
                     /locus_tag="RJE42_002550"
     CDS             1031..2305
                     /locus_tag="RJE42_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDV5877939.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(2466..3239)
                     /locus_tag="RJE42_002551"
     CDS             complement(2466..3239)
                     /locus_tag="RJE42_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDV5877940.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTYTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
BASE COUNT         1054 a          603 c          479 g         1154 t
ORIGIN      
        1 tatttgatgg ggcgctttca aaatattgaa aatcatatcc aaaatttaaa gaaatttatt
       61 tctctttatc ttcattttct tttttctctt cgttattaga tcctgtatat tcatttatct
      121 tatcttttac attttcaact tgttcattat cgctattttt aaatttttct actgcgtctt
      181 tagctttatc cataaaactc atattaatcg ctcctcttat atttgattag tttaattgaa
      241 cttatttttt aagtttatca attgcatcag ttattttgtt tttagcattt tcaacaactt
      301 cttttgcttt accagtcgct ttatcttgct gaccttcttt ttctaattct ttgttatcag
      361 taacgttacc tactgtttct ttaacattgc ctttaaattg atcgaactta ctttcgtctg
      421 ccatagtgaa acctccttag atgtatatat ttatatacca caaaggaggt tcgctaaaca
      481 ttataatatg aagtttttat gttatagtat agtatttata cgattaaata taaaacatgt
      541 atccgtctaa atcttcactt gtatctacat attccgctaa atatttcaat gttgtattat
      601 ctaaaacatc tctcactgca tctctcatgc gaatccatag ttgtttttgc gcaggtggtt
      661 ctgattcaat actttcaaca aatgtaattg gaccttctaa cagtcttata atatcccctg
      721 ctgagatttc ttccgctggc actcttaatt ggtatccacc tttagcaccg cgtacacttc
      781 gaattaaccc cgcatttctt aaaggaccta caagctgttc taaatataaa tcactcaaat
      841 tattttcttc agcaattgac tttaatgata tacatccttg cccctctttt ttagcaagag
      901 aaatcatcaa tgtaagtcca tatctccctt tagtagaaat tttcattgta taacctcact
      961 taattcgaat attgatattc ccattttagc attttttgag ttaagatagt ataagaaagg
     1021 tgtgacaaat gtgagtacag aaccattagc gtcgagaatg cgcccaaaaa atatagatga
     1081 aatcatttcc caacaacact tagttggacc aagaggcatt atcagaagaa tggttgacac
     1141 aaaaaaatta acttcaatga ttttttatgg tccacctggt ataggcaaaa caagtattgc
     1201 caaagcaatt tcgggcagta cacaatataa atttagacaa ttgaatgctg taactaacac
     1261 taaaaaagat atgcaacttg ttgttgaaga agctaaaatg tctggtcaag ttatcttgtt
     1321 attagatgaa atccatcgac tagataaagc caaacaagat tttttattac ctcatttaga
     1381 aaatggcaaa atcgtcttaa tcggtgctac aacttcaaat ccttatcatg ctatcaatcc
     1441 agcgattcgt tcaagagcgc aaattttcga gttatatcct ttaaatgacg aagatgttcg
     1501 ccaagcgtta actcgtgcaa tagaagatga agagaatggt ttgaaaacat atcaacccaa
     1561 aattgatgaa gatgctatga cctacttttc tacacaaagt caaggtgatg ttcgtagtgc
     1621 gctaaatgca ttggaattag ctgtattaag cgcagataat gacaaggacg gttatcgaca
     1681 tgttacattg caagatgcta aagactgctt acaaaaaggt gcatttgtaa gtgataagga
     1741 tggtgacatg cattacgatg ttatgagcgc tttccaaaaa tctatccgtg gtagcgacgt
     1801 caatgccgct ttacattatt tagcacgatt aattgaagct ggagatttac ctacaatagt
     1861 tcgacgatta cttgtaatta gctatgagga tataggctta gcctcaccta atgctggtca
     1921 gagaacactt gctgctattg aatcagcaga acgtctaggt ttaccagaag ctagaatccc
     1981 actaagccaa gcagtaatcg aactatgctt atcacctaag tcaaattcag caatgagtgc
     2041 cattgatagt gcattgtccg atattagaaa cggtcatgtg ggccaaattc caaaccattt
     2101 aaaagatgga cattatcaag gtgctaaaga tctaggccga tctattggtt acaaatatcc
     2161 acaccaatat gttaatggct atgtttcaca gcaatattta cctgataaac ttaaaaacaa
     2221 aatttattat gaaccaaaaa cgacatctaa aagtgaacaa caactcaaag aaatatataa
     2281 caatttactt aaacaaaggc cataaaaata agattaaatc gattaccttt gatattttag
     2341 ctataatcaa aacaaaactg actagtacac gaggcatatt ctactatgct tgtgaactag
     2401 tcagttttag ttttatggta tggttacact tgccttacgt tgaaaattat gactttcaat
     2461 tttaattatt gccctttgtc tttaatgcga cgaactggaa tatcttttaa aacgtcattc
     2521 accacataac ttgcacaaat taatccaaca acacttggca caaaggcatt tgaagaaggt
     2581 ggcatttgtc cttttcgatt gatagcattt ttatctccaa ctatatcttt tacatcttct
     2641 cttatgacaa ttggactttc atctgaaaat acaactggaa ttcctttacg aattcctagt
     2701 tttttcaatt tttgacgaat aattttggcc attggatcgg tatatgtttt agagatatct
     2761 gaaattgtaa aacgtgttgg atctgtttta tttgcagcac ccatactgga aatcacttta
     2821 atccctcggt caagacactc tttcattaag tgtactttgt acattattgt atcacttgca
     2881 tctacaaaat aatctatatc gtaattatcg aaaatttctt catatgtctc ttctgtataa
     2941 aacatatgta aaggcgtgac tttacaatct ggattaatta atttaatacg ttcttccatc
     3001 aaagaaactt tactttgtcc taccgttgta gttaaagcgt gtaattgtct gtttacattt
     3061 gtaatatcaa catcatcttt atctattaat ataatatgac caatattcgt tcttgctaat
     3121 gcttccgcag caaatgaacc aacacctcca acgccaagta tgacaacagt ttgttgcttc
     3181 aataaatcta aaccttgttg tccaatcgct agttcatttc ttgaaaattg atgtttcatt
     3241 attttacctc tttcactgat ttatacataa gtacatagta acttaaaatt
//
LOCUS       DAPIDM010000191         3253 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000191 DAPIDM010000000
VERSION     DAPIDM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.193"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(193..1128)
                     /gene="trxB"
                     /locus_tag="RJE42_002552"
     CDS             complement(193..1128)
                     /gene="trxB"
                     /locus_tag="RJE42_002552"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV5877941.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIETKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1195..2634)
                     /locus_tag="RJE42_002553"
     CDS             complement(1195..2634)
                     /locus_tag="RJE42_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV5877942.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVIENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            complement(2642..3127)
                     /locus_tag="RJE42_002554"
     CDS             complement(2642..3127)
                     /locus_tag="RJE42_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV5877943.1"
                     /translation="MRKFLSKTYHHTNPLWHVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            complement(3135..>3253)
                     /locus_tag="RJE42_002555"
     CDS             complement(3135..>3253)
                     /locus_tag="RJE42_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV5877944.1"
                     /translation="SILLILISISLIVYRRIKYNPPLYSKVGALPWPTKKVK"
BASE COUNT         1065 a          611 c          415 g         1162 t
ORIGIN      
        1 aaagttataa gtttaatata acatattaaa aaactagaac tcattaaaac gcacaaatga
       61 tacgtactaa gttctagttt tctatataat ttgctttaat ttaaaattaa gacattattt
      121 taacaatgtg ataacactat taaaataaat atccaaataa tttacagctc aacatcttaa
      181 ttcgacttcg aattaagctt gatcgtttaa atgttcaata tattccgctg cactttgcgc
      241 tgcaatacta ccatcgccag tagcagtgac aatttggcgt aaacctttgt cgcgaacatc
      301 tcctgctgca aaaatacctg gtactgatgt tgtcatatca tcttttgtct caatataacc
      361 aacatcattt gtaataccta agtctttaaa tggcgctgtt aatggtttca taccaatata
      421 gatgaataca ccatcagcct cgtgtgtttc ttctgaacca tcttttgtag acgttaatgt
      481 cacagaaccc actttgccgt ctttttcatt aattgatttc aaagtatgac tccaaataaa
      541 gtcgatttta tcatttttga atgctctatc ttgcaaaata cgctgtgcac gtaactcatc
      601 acgacggtga acgattgtta ctttgtcagc aaatttagtt aagaatgttc cctcttctac
      661 tgctgaatca ccaccaccga taacgaatag gcgcttgttt ttaaagaatg caccatcaca
      721 tactgcacaa taacttacac cgcgtccacc aagttcttgt tcacccggaa caccaatttt
      781 cttgtattct gcacctgtag caataatgac cgctttagct gttaattctt tgttaccaaa
      841 gttaatcact ttatattcgc ctttatcttc tacagattta atatctccat attgataaac
      901 tgcaccaaac tttttagcgt gttcaaacat ttttgtagat aaatctggac ctgtaatcat
      961 ttcgaaacca gggaagttct ctacttcttc tgtattagcc atttgaccgc ctggaatacc
     1021 tctttcaatc ataactgttt ttaaattagc acgtgatgcg tatactgcag cagtcatacc
     1081 agctggacct gcaccgataa ttgctatatc aaaatctatt tcagtcattt tattaacgcc
     1141 tcctcattat taatcattat gcgcattata taataaatct aacttttcat aaatctatat
     1201 gctcaagaga aattcaatca ttttgttcag tttatattgt gttatgccta accatgttgt
     1261 aatttgcttc tttgtaacgt ttcgaggttg atttttaaaa tacaaataaa taaacgcacc
     1321 gatatatggc tcaacatcag ttaaatctac tttttcagca attatgagtt caccttgatt
     1381 aatccatgca accattaaat cattttcaat tacaaataat tcattatggt aaagcgttaa
     1441 caagccgcga tgaatgaagt ctaatttatt aagcgttaaa ccttgaacta aatacgttaa
     1501 atacaatttc tcataattat ttagattttc cagaacctgc caaatacttt ccgtcatcac
     1561 aatttctttg ccatttaatt gatccaataa aaaaatacca tataaacgat aatgactgtc
     1621 atcactttga agtaatggca aaatatgttg gtcaaaaact tctttgctat tttcaattac
     1681 ccaaggcgca tgtccaattt ccactttaga aatttgcttc aatttatccc agtagtaatg
     1741 actttcgtct tcttcaccta aataataata attatatgct aaagcattgt acatttgaat
     1801 tgataaaaat ttcccttttt tatataaagg gtacaacaat tgttgtgatg cacgatactg
     1861 ctttaaataa cttaaaacaa tacctaattt aaaactttca tcgtcattca taggtacaac
     1921 tttatttaat atttttaaat acttttggta ttgttcattt tccttagtgt tataaagtag
     1981 caaagtataa tggcataatg catatatatc tgtactatcc tcttgtaata gtgcttcgta
     2041 caatgcttta gccgtatcat attcgccgag atataaataa cacattgcca gtaaattacg
     2101 tacgacacga tgatcttgaa cttcttgtgg ttgatgtaag acataattct ttgcatcttt
     2161 taatcgacct tgagaaaaaa gatattggaa tagtatttga actacaaaca attgagcttc
     2221 aagttcaatt tgttcttcac tttgatattt cacatcaaac atttgatcta attcatctct
     2281 ataatcttgg tcgtcgctaa caataacata attaatacca aacaagaatg ccttgtttgg
     2341 ttcgttaact tcaatgttga gctgactcaa ttcataaaag ctatcttcta gatgcctatt
     2401 ataaataata ttgtcataaa ataaatgttc tgctttttta gcaataccaa gttgcactag
     2461 acattgtgca taatcaattt gaatttccaa atcatcaggt gacaattcca acactttttc
     2521 aaagtattca gcagctcgtt tatattctct ttgtttaaac ttctgattag ccattttacg
     2581 ataaaatgca tcatcaaaag tcattggaat tacattatta ttcttttgcg ccataaaatt
     2641 gtcacctcct aatcattttt atatacatag ggttgccata tgcaaatcca ttatccggta
     2701 tatcttttga aacaaccgta ccagctgcaa cttttacatt gtcaccaatc gttataccgg
     2761 gtaaaatggt agcatttgca ccaatcaaag tgttagatcc tatcgtcact ggtccataac
     2821 gaaattcatc aactaatgct tcatgcgtca aaattgttac attgtaacca ataacactgt
     2881 tactaccaat cgtaatcagt tctggataaa aaatatctaa cattacttta taagctatcg
     2941 acgattgatt accgatatta atatttaaaa gttgtttata tatatgtctt ttcagtacca
     3001 tacttggaat aaatttcgaa aattcaatga tgattacatt cttaaaaact ttcgaaaatt
     3061 taacaagacg gtatacatgc cataaagggt ttgtatgatg atatgttttt gataaaaatt
     3121 ttctcaaaat atcactactt cacttttttt gttggccatg gaagcgcccc aactttgcta
     3181 tacaacggtg gattatactt aatccttcta tatacaatta aacttatact tattaaaatt
     3241 aaaagaattg ata
//
LOCUS       DAPIDM010000192         3116 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000192 DAPIDM010000000
VERSION     DAPIDM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.194"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            464..2128
                     /gene="alsS"
                     /locus_tag="RJE42_002556"
     CDS             464..2128
                     /gene="alsS"
                     /locus_tag="RJE42_002556"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDV5877945.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPSLGVPSEHDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2165..2869
                     /gene="budA"
                     /locus_tag="RJE42_002557"
     CDS             2165..2869
                     /gene="budA"
                     /locus_tag="RJE42_002557"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDV5877946.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEM
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
BASE COUNT         1129 a          458 c          585 g          944 t
ORIGIN      
        1 ttataaaaac aatcgctaat ataagtatga aatgtataag tcacagttag ttctaagtct
       61 tgctgtggct tttttatcga aatgacaatt tcatgttgct ataaaacagc attttcagcc
      121 cgccataaaa cgacaatttc aaaccgtcat tgactaaaga cctcatcctc aaatatgcta
      181 acaatcctcc acacaaatca atccaacatc cctcataatc acaacgcaca aaatctactc
      241 atgcattttt ggaatactta gtattacaaa taacgatttt tattcatcta acaaaggtta
      301 tataatgtac tgaaggcaat ttttatgtat tgcaaatcta atcgtacatg taaaattttg
      361 ataaacatca ttaattttgc gtaactatca ttagattaca aatcacaatg taattacatg
      421 taatacacat ctatacatca catttgaagg gaaatgaata taaatgactg ataaaaagta
      481 cactgcagcc gatatggtta ttgatacttt aaaaaataat ggggtagaat atgtttttgg
      541 tattccgggt gcaaagatag actatctatt taatgcttta attgatgatg gtcctgaact
      601 tattgtcact cgtcatgaac aaaatgccgc aatgatggcg caaggtattg gaagattaac
      661 aggcaagccg ggtgtagtac ttgttacaag tggccctggt gtaagtaatt taactaccgg
      721 actattaaca gctacatctg aaggggatcc tgtattagcg ttaggtggcc aagtgaaacg
      781 taatgattta ttacgattaa cgcatcaaag tattgataat gctgcgctat taaaatactc
      841 atcaaaatac agtgaagaag tacaagatcc tgaatcatta tcagaagtta tgacaaatgc
      901 aattcgtatt gctacttcag ggaaaaatgg cgcaagtttt attagtattc cgcaagacgt
      961 gatttcttca ccagttgaat ctaaagcgat atcactttgc caaaaaccaa gtttaggggt
     1021 accgagtgaa catgatatta atgatgtcat tgaagcgatt aaaaatgcat catttcctgt
     1081 tttattagct ggaatgagaa gttctagtgc agaagaaaca aatgccattc gcaagctagt
     1141 agagcgtacc aatttacccg ttgtagaaac attccaaggt gcgggtgtga ttagtcgtga
     1201 attagaaaat catttctttg ggcgagtggg tttattccgt aatcaagttg gtgatgaatt
     1261 attacgtaaa agtgatttag ttgttacaat cggttatgat cctatagaat atgaggctag
     1321 taactggaat aaagaattag atacgcaaat aatcaatatt gacgaagttc aagctgaaat
     1381 tactaattat atgcaaccga aaaaagagtt gattggtaat attgctaaaa cgattgaaat
     1441 gatttctgac aaagtagatg aaccatttat aaatcaacaa catttggatg aattagaaca
     1501 attaagaaca catattgatg aagaaactgg tatcaaagcg acgcatgaag aaggtattct
     1561 acatccagtc gaaattatag aatctatgca aaaagtatta acagatgata ctacagtaac
     1621 agttgatgtt ggaagtcact atatttggat ggcacgtaat ttcagaagtt acaatccaag
     1681 acatttatta tttagtaacg gtatgcaaac gcttggtgta gcattaccgt gggcaatttc
     1741 agctgcgctt gtacgcccta atacgcaagt tgtatccgtt gctggcgatg gaggcttttt
     1801 attttcatca caagatttag aaacagccgt acgtaaaaat ctaaacatta ttcaacttat
     1861 ttggaacgat ggaaaatata acatggttga attccaagaa gaaatgaaat ataaacgttc
     1921 atcaggtgtc gacttcggtc ctgtagattt cgtaaaatat gctgaatcat ttggcgcaaa
     1981 aggtttacga gttactaatc aagaagaatt agaagcggca attaaagagg gctatgaaac
     2041 agatggtcca gtattaattg atatacctgt aaattacaaa gataatatca aactttcaac
     2101 aaatatgtta cctgacgtat ttaactaaaa taaagataaa tgttaaagag gagtgggaaa
     2161 ttttatgact aatgttttat accaacatgg tacattaggc acattaatgg caggattatt
     2221 aaaaggaact gcatcaataa atgaattatt gcaacatggt gatttaggta tcgctacact
     2281 aacaggttca aatggtgagg tcatcttttt agatggaaaa gcctatcatg caaatgaaca
     2341 taaagaattt gtggaattaa aaggtgatga gttaacacca tatgcaactg taactaaatt
     2401 cgtagcagat acaagctatg aaacgaaaga taaatcttct gaagcagttt ttgcagaaat
     2461 taaggaaatg atgttgagtg aaaatttatt ttctgcagta aaaatttcag gcttgtttaa
     2521 aaaaatgcat gtacgtatga tgccggcaca agagccgcct tatacacgtt taattgactc
     2581 agctagaaga caacctgaac aaactgaaac gtatgtcaaa ggttcagtcg ttggtttctt
     2641 tacaccagaa ttattccatg gtatcggatc agcaggattc catgtacact ttgcaaatga
     2701 tgatcgtaac tttggtggac atgtcttaga ttttgaagta gaagatgtta aagtagaaat
     2761 ccaaaatata gaaacatttg aacagcattt tccaattcaa gatgaagatt tcataaatgc
     2821 aaatattgac tataaagata ttgcagacga aattagagaa gctgaataat gaatgcggaa
     2881 atgtaatgat ggattataaa aattgaagca ttgacttcat aatgttcgat ttggaaattt
     2941 agcaatgata aaaggttgat taatacggtt aagcatgatt acaatagagc atacgtctaa
     3001 gtaataaaat aaatcccttc actattaacg gtagtgaggg gatttatttg tgttctagct
     3061 atttgagatt tgctgttatg ttagataata aattgtggta gacattaata tagaat
//
LOCUS       DAPIDM010000193         3114 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000193 DAPIDM010000000
VERSION     DAPIDM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.195"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            219..809
                     /locus_tag="RJE42_002558"
     CDS             219..809
                     /locus_tag="RJE42_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV5877947.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            1026..2372
                     /locus_tag="RJE42_002559"
     CDS             1026..2372
                     /locus_tag="RJE42_002559"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV5877948.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WVSMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAMQQKESKVKEISARRIFSFE"
     gene            2394..>3114
                     /gene="ccsA"
                     /locus_tag="RJE42_002560"
     CDS             2394..>3114
                     /gene="ccsA"
                     /locus_tag="RJE42_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDV5877949.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITLLYGSYILLRIN"
BASE COUNT         1145 a          410 c          548 g         1011 t
ORIGIN      
        1 aaactgaacc agaactatac gacgcagaag gtaatttaat taataatagt aaaacatcag
       61 cttaatcatt gatgtgttat aaaagtgaaa agctccaatc aaagttgacg ctcaaatagt
      121 ttaactttga tcggagcttt tttataatgt ataccgaaag gttcaaatat tatataatgg
      181 taaagttgaa agagaatata aaaaagtgag gatataaaat gaaagttaat cctaataata
      241 tagaattaat cattagtgca gtaaaggaag aacaatatcc agaaacagaa ttgtctgaag
      301 ttgcacttag cggtcgatct aatgtaggta agtctacatt tatcaatagt atgattggca
      361 gaaaaaatat ggcacgtaca tcacagcaac ccggcaaaac gcaaacgtta aatttttata
      421 atatagatga acaacttatt tttgtggatg ttccaggata tggatacgct aaagtaagta
      481 aaacacaacg tgaaaaattt gggaaaatga ttgaggaata tataactaag agagagaatt
      541 tgcaattagt tattcaatta gttgatttaa gacatgatcc aacacaagat gatatcttaa
      601 tgtacaatta tttgaaacat tttgatattc ctactttagt tatatgcact aaagaagaca
      661 aaattccaaa aggtaaagtt caaaagcata ttaaaaatat taagacacaa ttagatatgg
      721 atccagacga tacaatagta agttattcat caattcaaaa taataaacaa caacaaatat
      781 ggaatttaat tgaaccgtat atttcatagt ctttgtacga caaaacttat acaaaaattt
      841 taaaaataat gtaagcacga aacttttaat tagtacacaa ttgataacat ttttcaacgt
      901 tcatcatttt gtcaaaaact taaaagtaaa ttagaaagat tataatttat ttaagcatcg
      961 tacttaattg gattttaaat tatgttataa tatttgtatt gttagtatat atgggggcgt
     1021 ttcaaatgca ttttattgca attagtataa atcatcgcac agctgatgtt gcactaagag
     1081 agcaagttgc ttttagagat gatgccttac gaattgccca tgaagattta tatgaaacta
     1141 aatctatttt agaaaatgtc atattatcaa catgtaatcg aactgaagta tatgctgttg
     1201 ttgatcaaat tcacacaggt cgttactata ttcaacgatt tctagctcgt gcatttggat
     1261 ttgaagtaga tgatattaaa gcaatgtcag aagtaaaagt gggggacgaa gcagtagaac
     1321 atttattgcg tgtcacttct ggtttagatt caatcgtact tggagaaact caaattttag
     1381 gtcaaataag agatgcattt ttcttagcgc aaagcacagg tacgacagga acaattttta
     1441 atcatctatt taaacaggca attacttttg caaaaagagc acataatgaa acagatatag
     1501 ctgataatgc tgtaagtgtg tcttatgctg cggtcgagtt ggcgaaaaaa gtatttggca
     1561 aattgaaaag taagcaagct atcattattg gtgcagggga aatgagtgaa ttatcactat
     1621 taaatcttct tggttctgga attactgata ttacagtagt aaatagaaca attgaaaatg
     1681 ctatgaaatt agcagcaaag catcaagtga aatatgatga actatcatca ttaccaaatt
     1741 tacttgaaag tgcagatatt gtgattagtt caacgagtgc acaatcttat atcattacaa
     1801 atgaaatgat agaaagaatt gcagaaaata gaaagcaaga ttcactagta ttgattgata
     1861 ttgcagttcc tcgagatatt gaacctggta taagtgccat cacaaacatc tttaattatg
     1921 atgttgatga cttaaaaggt ttagttgatg caaacttacg tgagcgacaa ttagcggctg
     1981 caacaatttc ggaacaaatt cctgcagaaa tacatgcaca caatgagtgg gttagtatgt
     2041 tgggtgttgt tccagtgatt agagctttac gtgaaaaagc tatggcaatt caagcggaaa
     2101 cgatggacag tattgatcgt aaattgccag ggttaagcga aagagaacgt aaaattatat
     2161 ctaaacatac aaaaagtatc atcaatcaaa tgttgaaaga tcctattaaa caggccaaag
     2221 aattaagtag tgataagaaa agtaatgaaa aattagagct atttcaaaat atatttgata
     2281 tcgaagctga atgtcctcat gagcaagcga tgcaacaaaa agaaagcaag gtcaaggaaa
     2341 tttcagcgcg acgtatcttt agttttgaat aagcatatta atatggtgat aatatgcaag
     2401 aaaacctgtt tattcgattc aatgaaatta tattattaat atacttaatc agtatcattt
     2461 gctattttta tgattttgtc caaaaaagtc ataagattag aagtttaggc atatatttat
     2521 tggggattgt ttgggtttta caaacaatct ctttatctat ttttattata caaactagac
     2581 atattccatt agggtctatt tcagatgtat tctatacttt aagttggctg attatttcaa
     2641 tctctttaat tcttaacctt atcaaagttt tgaatttctc cgtgtttttc ttgaatttaa
     2701 tagggttaac acttttaggg atgaatacat ttcaaccgac acattatact aataaggttc
     2761 agaaaattgc tgttgtagac gagttactgt tagtccatat cggtctagct gtgttgagtt
     2821 atgtcttctt tgccttagct tttgtcaatg cattactgta tatcatgcaa taccgtaatt
     2881 tgaaggaaaa gcgttttgat cagaaatact ttagaatagg tagcgttgct acacttgaat
     2941 caattgtgtt ttactcaacg ttaagtgggt ggataattct catatttagt attattttag
     3001 gaacccagtg gggtgtaatt tcagttggtg aacgtatatt cattgacccg aaggtaatac
     3061 tctcttcaat tattacattg ttatatggaa gttatatttt acttagaatc aaca
//
LOCUS       DAPIDM010000194         3064 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000194 DAPIDM010000000
VERSION     DAPIDM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.198"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(200..676)
                     /gene="folK"
                     /locus_tag="RJE42_002561"
     CDS             complement(200..676)
                     /gene="folK"
                     /locus_tag="RJE42_002561"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV5877950.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(673..1038)
                     /gene="folB"
                     /locus_tag="RJE42_002562"
     CDS             complement(673..1038)
                     /gene="folB"
                     /locus_tag="RJE42_002562"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDV5877951.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNLVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(1016..1819)
                     /gene="folP"
                     /locus_tag="RJE42_002563"
     CDS             complement(1016..1819)
                     /gene="folP"
                     /locus_tag="RJE42_002563"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV5877952.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMITVEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHRMFQVVAKYDAEIVLMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEIMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(2035..2967)
                     /gene="cysK"
                     /locus_tag="RJE42_002564"
     CDS             complement(2035..2967)
                     /gene="cysK"
                     /locus_tag="RJE42_002564"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV5877953.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
BASE COUNT          938 a          657 c          401 g         1068 t
ORIGIN      
        1 caacaaaagc ccaaataaat tacagcaatt catcaaatat tgcttacttt gattttatga
       61 aataacttaa ttctaacaca tactaaatca tcatatacta attcgaaatc aaatgcattt
      121 agagataatc aaaatgcgga aacatctcca ataatcaatc atctattccc aataaattat
      181 gaatgttctc aacaatacat tatttatatc tctttacact gtcatcgaca aaaactaaat
      241 ctttcacttt caatttcgaa cgtggttcta tgacatttgc tgctatatca tttaatggga
      301 ttaaaacaaa tgcacgttca ttcattctcg gatgtggcac cgacagtttt ggcaaatcta
      361 tcatttcttc tccatacaac aaaatatcca catctaaagt tctaggaccc catcgttcct
      421 ttctaacacg gtgtaaacat tcttctgtct tcaaacaaca ttccaacagt tgtaatactg
      481 tgagtgttgt ttgaatttca acacacaaat ttaaaaagtt aggttgctca gtatacccaa
      541 ctggtgctgt ttcataaatc ggagaaatat tagatacgtt aataccatca tattcattca
      601 aaatctttat agcatcgttt aactggcttt ctctatcacc aatattacta cctaatccta
      661 agtatgcttg aatcatttat tctccctcac aatttcgata cctactccat cataatgacc
      721 cggaatcggt gggttttctt tagtgattct cactttcgtt tccattacaa gattatattg
      781 tgaatttata cgatttgcaa tacgttcagc tagatgttca agtaaattaa cggccttacc
      841 ttccataatt gatttaacct cttcgaacac ttcaccataa tgaactgtat caataacatt
      901 atcagtacga ccagcttcag ctaagtctac tttcaaagtt acatccactt tgaaaatttg
      961 tcctatttca ttttcagctg ataaagcacc atgatatcca taaaagcgca tacctttaag
     1021 aaagattgtg tcttgcattt tcattctcct ttaaaaaatc tataccttta gctaatttag
     1081 cattcaactc gacattatga acgcgtactg ctctaacgcc tttcataata ccatatgcag
     1141 tcgtagctgc agttacttca tctctttcaa ccggtgttgt atcataaccc atcatctctt
     1201 tagtgaaacg tttccggctt gtcgctaata aaactggata ttctgttgca acaagttcat
     1261 ccagtcttgc cataatttcg gcttcttcat ttctagtttt agcgaaacct atacctggat
     1321 ctagccaaat tttatttgaa ggtataccag ctattttagc ctgatgtgct tgtgctaaca
     1381 aagatgttaa catttcttcg acaaccggtt catcacgatt accattgcca ttatgcatta
     1441 aaacaatttc cgcatcgtat ttggctacaa cttgaaacat gcgatgatca taaaggcctg
     1501 cccattgatc attaataata tcaacaccta gctttaaaca agcttcagca acttcactac
     1561 gaaaagtatc aaccgaaatt tttacatcaa aaccgacaat cgcttcaaca actggtaata
     1621 ctctattcaa ctcttcttcc acagtaatca tttcatggcc gggtctcgtt gaaacacctc
     1681 caacatctat aatgtcagca ccttcatcca tcatcgcttt cactctagtt actgctgatt
     1741 caacattatt aaattttcca ccatctgaga atgaatcagg tgtgacgttt aatatgccca
     1801 taatttttgt tttagtcatg ttaaccaccc tttaattatg tattatttaa ctctaataaa
     1861 atttacttct gcgatttact agttattagt atatacgttt attatcttat cattcactgg
     1921 atgtttttat cagaaaaatg tttgatttaa cgctcatatt tttaaaatta tacatggtag
     1981 aaaaaaagag caagttatct caaaaagata actcactctt ttaaatgacg ttaattagtc
     2041 atcgaatgaa tataaaggtg ttgataagta gcgttcacca ttactcggca atactgttac
     2101 tactgtttta ccttttccta attcttttgc tttttgaatg gcagcataaa tcgcagcacc
     2161 tgatgaaata cctgctaaaa taccttcctc tttagcaact cgacgagaca tttccatcgc
     2221 tgtatcattt cctactttaa taatactgtc atagatttct gtattcaaag tacctggaat
     2281 aaatccagca cctaaacctt gtaatttatg tggacctggc tcaccaccgc tcaatactgg
     2341 agaagcctca ggctctatag caacaatttc gatgttagga tattcttttt tcagaacttt
     2401 acctacacca gataacgtac cgccagtacc aacaccagct aggaacgcat cgatagtttt
     2461 cccttcaaat tgttgtaata actcaggacc tgtagttaac tcatgaactt cagggttcgc
     2521 agggttttca aattgttgtg gctcgaagta accatgttct tcttttaatt ctttagcttt
     2581 tttaattgca cctttcatcg cttctgatcc aggcgttaaa actaattccg caccgtatgc
     2641 ttttaataaa ttacgacgct cttggctcat tgtttcaggc atagtaaata ctgctttata
     2701 tcctttagca gcacatacaa atgctaaacc gatacccgta ttaccactag ttggttctac
     2761 aattgtatcg ccaggtttaa ttttgccttc tcgctctgct ttttcaatca ttgctaaagc
     2821 aattctatcc tttacagaac cacctggatt ttgatattcc aattttacat aaacatctgc
     2881 tgcattgtca tctactacat ttctcaattt gactaccggt gtaccgccaa taatttgagt
     2941 aatattatct actggttttt gtgccattaa gaacactcct ttttagttag ttgttgaaat
     3001 cttagtttat ttatcggata tagaattatt ttatcaaata aattctagaa agcaaacaaa
     3061 atta
//
LOCUS       DAPIDM010000195         3025 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000195 DAPIDM010000000
VERSION     DAPIDM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.199"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(63..248)
                     /locus_tag="RJE42_002565"
     CDS             complement(63..248)
                     /locus_tag="RJE42_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877954.1"
                     /translation="MLSNKRVEKEATCKHECFSKKLLTLNCKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(337..1401)
                     /locus_tag="RJE42_002566"
     CDS             complement(337..1401)
                     /locus_tag="RJE42_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDV5877955.1"
                     /translation="MLTVDQVKELIGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     VAMAQLGGAPQLDLQMAVVNVLKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTEHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQAE
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1549..2991)
                     /gene="lmrS"
                     /locus_tag="RJE42_002567"
     CDS             complement(1549..2991)
                     /gene="lmrS"
                     /locus_tag="RJE42_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDV5877956.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLVGSIVAAMAPNFG
                     VLMVARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFIFGLISISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGAMIISSIIIYLFIRRQFNISNALLNLSVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFSSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
BASE COUNT          987 a          594 c          412 g         1032 t
ORIGIN      
        1 ttttattata cttcatttat ttcttgaagt caataagttt ttgaatgatt actttattta
       61 ttctatattg tttttatagt tatttcaatg gtaagtttta cacttttgaa attcttcttt
      121 aaaaacaact gcgtcgtttt tgacgacttt atcatattat caactttgca atttaaagtc
      181 aataactttt ttgaaaaaca ttcgtgtttg caagtcgctt ctttttcaac gcgtttattg
      241 cttaacacaa gaacttattt taccagcatt ccaaaacaaa tcaacataaa aacgtacaaa
      301 ataaaagtaa ttttgtacgt ttagcatatt ttatacctat ttatttgtgg cagctattac
      361 tttttgtgct atagagctat aaattttacc taatctgtct tcagcttgat aaattgaagg
      421 tgcaaagtct tttggattcc aagacggttg ctctaatggt aattcaccaa gtaattgagt
      481 attgagttca tcagctaact tagtaccgcc acctttgcca aagacatact ctttattccc
      541 cgtctcttta ctttcaaaat aactcatatt ttctataaca ccaagaatag aatgttctgt
      601 atgctttgcc atcgcacctg cacgcgctgc aacaaatgct gctgtaggat gaggtgtcgt
      661 tacaataatt tccttacttg aaggtaacat cgtatgaaca tctaaagcta catctcctgt
      721 tccaggtgga agatcgagta ttaaatattc aatgtctccc catttaactt ctgtaaagaa
      781 attcgtcaac attttaccta acattggccc tctccatata actggcgcat tttcttccac
      841 aaaaaaggcc attgatataa ctttaacgcc atgacgttca actggaatta cttccttccc
      901 tttaattcca ggcttttcat caatccccat catatctggt acactaaatc catatatatc
      961 ggcatctact aatccgactt ttttaccttc acgggctaag gcaacggcta aatttactgc
     1021 aacagtagat ttaccgacgc cacctttacc tgaagcaata gcaataaatt caactggatt
     1081 attttgagat aataagcctt ctatcgtttt aggtttattt tcttcttttg gtttaaattg
     1141 atttactttt tcttctggta acgtttcaaa tcgtataccc acagtttttg caccattttc
     1201 ttttaaaaca ttaacaactg ccatctgtaa atctaattgc ggtgcaccac ctaattgtgc
     1261 cattgccact ttaacactta catgttcttt ttcttcctta atagaaactt ctacaatacc
     1321 ttctgtttct tttaaaggta catctataat aggatcttta atttctccta ttagttcttt
     1381 aacttgatct accgttaaca aagcaaatcc cccttaatca atttaaaata tctacatcat
     1441 agcaaataac aatttttgct tagtctatca ataaaagtat aaaacttttc attaaataaa
     1501 aaaagaagtt agcctattat tttcaataga ccaactacta taattatttt aaaatttcct
     1561 tctattactt tctatttctt taggttctct tataaaaagc gctatcacat aagctgccat
     1621 aacaagcaca gctacaaaag caaatgagat atccacccct atagcaattg attgctgttt
     1681 aatttcagtt gcagttaaat gcgatggcga tgtagatgag aatatttttg cgccaaaact
     1741 catcaaagta accattaatg ctgttcctaa tgagccagcc attacacgac caaagttcat
     1801 aatagccgtg ccatgtgaga tttcttcatt tctcaaagaa ttaattcctg tagtatttat
     1861 cggcatcatg agtaaagaaa ctgaaaacat tctaattgca tatacgacta ttaaatacgt
     1921 ataagatgtg tctgtatgca agaaacacaa cataatagta gtaattgtta aaatcgtaaa
     1981 accagtataa atcagtggtc taggaccata cttatcataa aatttacctg taaaaaccga
     2041 cataatacca tttatgattg cacctggcat gataacaagt ccagacaata atgcagatag
     2101 agataagcta ttttgaacat agagcggtat taataacgct ggaccaacca ttgacatcat
     2161 tatgatcatt gagctaatcg tacataatgc aaatgttcta tttttaaaaa cacttaaatt
     2221 taaaagtgca ttgcttatat tgaattgtct acgaataaat aagtagatga taatagaact
     2281 aattatcatt gcaccaatga ctatcgggct agtaaatcct aggtcgcctg cactactgaa
     2341 tgcatataac attaatccaa atccaatcgt tgaatacatt acagagcgct tatctagctt
     2401 agtatatttc acttcattat agcttgaaat tgaaattaaa ccgaagatga atgcgactaa
     2461 agcaattcct acaattataa taaatggtac tcgccaactc gcttgatcaa tgattaatcc
     2521 agtaacagta ggcccgattg ctggtgcaaa ttgaataact aaaccagcta atcccattgc
     2581 aaatcctcta tgttctttac tgaataacgt aaacaatgtg aattgcatta aaggcatgag
     2641 tacgcctgcc cccatagctt ggatgacacg cgctaccatt aatacgccaa aattgggtgc
     2701 catggctgca acaattgatc ctactaaaaa tgtccccatc gctgctaagt ataaaggtct
     2761 agttttaatc ctgtccatta agtatgctgt tagaggtatc atgacgccat taacaagcat
     2821 aaacccagta actagccatt gcgatgtact ttcattaata tttaattctc tcattatact
     2881 aggtaacgct gtatttaata atgtttgatt taaaatagct acaaacgcac taatcaacat
     2941 aacagcaact atgaagtttc gtctccgggt tgttaattca actttagcca tcataattcc
     3001 tcctttgtaa caaatttacg tttat
//
LOCUS       DAPIDM010000196         2979 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000196 DAPIDM010000000
VERSION     DAPIDM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.201"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            229..1470
                     /locus_tag="RJE42_002568"
     CDS             229..1470
                     /locus_tag="RJE42_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDV5877957.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLN
                     YDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQ
                     FFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT
                     AFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIK
                     QKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQK
                     QIKAAQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKP
                     LKENIEIALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELY
                     SEQEQQQEQQKNQKRDRGMHL"
     gene            1997..2951
                     /locus_tag="RJE42_002569"
                     /pseudo
     CDS             1997..2951
                     /locus_tag="RJE42_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT         1213 a          383 c          545 g          838 t
ORIGIN      
        1 gacgattatt gtatttaaac gttctgaaaa gcagtttgaa tagttatatt atattttggt
       61 ttagaactat gagtggctag cattttgcca ctcatttttt gcgttagcaa aaacaggttt
      121 aagcctcgca gagcacacgt attaacgact tattaaaaat aagtctagtg tattagactt
      181 aaactattaa atacacatga aacctttgtg cttaggagtg atttttatat gtcttattcc
      241 attgttagag tttcaaaagt taaatctgga acaaatacaa cgggcataca aaaacatgtt
      301 caaagagaaa ataataatta tgaaaatgaa gatatagacc atagtaaaac ttacttaaat
      361 tatgatttgg taaatgctaa taaacagaat tttaataact tgattgatga aaaaatcgaa
      421 cagaattata caggcaaaag aaaaattaga acagacgcga ttaaacacat tgatggttta
      481 attacatcag acaatgattt ctttgataat caaacgccag aagatacaaa gcagtttttt
      541 gaatatgcta aagagttttt agaacaagaa tacggtaaag ataatttatt atatgcaaca
      601 gttcacatgg acgaaaaaac accacatatg cattatggcg ttgttccaat aactgatgat
      661 ggtcgtttaa gtgctaaaga agttgtaggt aataaaaaag ctttaacagc gtttcaagat
      721 agatttaatg agcatgttaa acaacgagga tatgatttag aacgtgggca atcaagacaa
      781 gtaacaaatg ctaaacatga gcaaataagt cagtataaac aaaaaacaga atatcataag
      841 caagaatatg aacgtgagag ccaaaaaaca gaccatataa agcaaaagaa cgataaatta
      901 atgcaagagt accaaaaatc gttaaatacg cttaaaaagc ctataaatgt tccgtatgag
      961 caagaaactg aaaaagtagg tggtttattt agcaaagaaa tacaagaaac tggaaatgtt
     1021 gtaataagcc aaaaagattt caatgaattt cagaaacaga taaaagctgc tcaagatatt
     1081 tcggaagatt acgagtatat aaagtctggt agagccttag atgataaaga taaggaaata
     1141 cgagagaaag atgatttatt aaataaagca gttgagcgta ttgaaaacgc agacgataat
     1201 tttaaccaac tttacgaaaa tgcaaagcca cttaaagaga atatagaaat agcgttaaag
     1261 cttttaaaaa tcttactaaa agagttagaa cgagttttag gaagaaatac ctttgcggaa
     1321 agagttaata agttaacaga agatgaacca aaactaaatg gtttagcagg aaacttagat
     1381 aaaaaaatga atccagaatt atattcagaa caggaacagc aacaagaaca acaaaagaat
     1441 caaaaacgag atagaggtat gcacttatag aacatgcatt tatgccgaga aaacttattg
     1501 gttggaatgg gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat
     1561 taatcccaag aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag
     1621 gaaattggaa taaagcaata aaaggagttg aagaaatgaa attcagagaa gcctttgaga
     1681 attttataac aagtaagtat gtacttggtg ttttagtagt tttaactgtt taccagataa
     1741 tacaaatgct taaataaaaa aagacttgat ctgattagac caagtctttt gatagtgtta
     1801 tattaataac aaaataaaaa ggagtcgctc acgccctgac caaagtttgt gaacgacatc
     1861 attcaaagaa aaaaacactg agttgttttt ataatcttgt atatttagat attaaacgat
     1921 atttaaatat acatcaagat atatatttgg gtgagcgatt ccttaaacga aattgagatt
     1981 aaggagtcga ttttttatgt ataaaaacaa tcatgcaaat cattcaaatc atttggaaaa
     2041 tcacgattta gacaattttt ctaaaaccgg ctactctaat agccggtaat agccggttgg
     2101 acgcacatac tgtgtgcata tctgatccaa aattaagttt tgatgcaatg acgatcggtg
     2161 gaaatctcaa ccgagacaac gctcaagccc tttctaaatt tatgagtgta gagccccaaa
     2221 taagactttg ggatattctt caaacaaagt ttaaagctaa agcacttcaa gaaaaagttt
     2281 atattgaata tgacaaagtg aaagcagata gttgggatag acgtaatatg cgtattgaat
     2341 ttaatccaaa caaacttaca cgagatgaaa tgatttggtt aaaacaaaat ataataagct
     2401 acatggaaga tgacggtttt acaagattag atttagcctt tgattttgaa gatgatttga
     2461 gtgactacta tgcaatgtct gataaagcag ttaagaaaac tattttttat ggtcgtaatg
     2521 gtaagccaga aacaaaatat tttggcgtga gagatagtaa tagatttatt agaatttata
     2581 ataaaaagca agaacgtaaa gataatgcag atgctgaagt tatgtctgaa catttatggc
     2641 gtgtagaaat cgaacttaaa agagatatgg tggattactg gaatgattgc tttagtgatt
     2701 tacatatctt gcaaccagat tggaaaacta tccaacgcac tgcggataga gcaatagttt
     2761 ttatgttatt gagtgatgaa gaagaatggg gaaagcttca cagaaattct agaacaaaat
     2821 ataagaattt gataaaagaa atttcgccag tcgatttaac ggacttaatg aaatcgactt
     2881 taaaagcgaa cgaaaaacaa ttgcaaaaac aaatcgattt ttggcaacat gaatttaaat
     2941 tttggaaata gtgtacatat taatattact gaacaaaaa
//
LOCUS       DAPIDM010000197         2856 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000197 DAPIDM010000000
VERSION     DAPIDM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.202"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..783)
                     /locus_tag="RJE42_002570"
     CDS             complement(<1..783)
                     /locus_tag="RJE42_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV5877958.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLS"
     gene            1125..2693
                     /locus_tag="RJE42_002571"
     CDS             1125..2693
                     /locus_tag="RJE42_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDV5877959.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSVFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGSGYNAVYQPDTKKLPNAIFWL
                     KTYPERLKQARAKK"
BASE COUNT          941 a          390 c          529 g          996 t
ORIGIN      
        1 tgataaccat gaacctaaga aaggaattaa gtttgcgaca ccagcaaata gtactaataa
       61 aggaatatat ggtaagtcaa taattgaata accgatatat aagaatatac ctaaaataac
      121 actgacagtt acttgacctt gaatgtaaga ttttaatgta aagtttaaat cagttaataa
      181 atctacgaaa aatactttac gttcaccttt gaaaaattta gcaacagctg ggataaattt
      241 ttcatggtct tttaacatat aaattaagaa gaatggaacc ataatcaata agaagatggt
      301 tgaaattaat gatgtaatgt actgtaatga attagataaa atattagtaa cgccatcacc
      361 cattgattta accatatttg taattctact tgttacatct tcaggtaatt tatccatttg
      421 agctagtgcg aatttaataa tttgctctgc ttctttttgt agggcaggtg tctgactaat
      481 taaattgttg atatttgaaa tgatgattgg tgcaacaaac gcaacaatta agccgataat
      541 agcaaacaaa gctaacatga ttgttgttat actagcccat cttggaaaac cgactttttc
      601 aagtaagttt tgaaaaggta gacagatata aaataaaaaa ccactaatta aaaatggaag
      661 aaatacagaa ccgatgattg tagctattgg agtaaatact tcgtgcactt ccataaatag
      721 tttgatgaga atgaacagca taatcagagc aatgccagtt cggaaccaaa ccttgtttaa
      781 cataaacgat gttcctccta tttttctaaa cttagttatt atttagtata tcctaaatag
      841 cagtgcttaa aaaggttaat attttttaat tatacttaaa ttaaccattc actttctatt
      901 ttaagatatt tactttcaat agtttgtgag acttttcaat gactcgtaag tctgcaaatg
      961 gatcaaacag taccaattta tatgaataac ttattttaat attaaacgtg tttaaataat
     1021 gaaatgatca tattattatt tataagaata ttctgaaaaa tatattgaag tgtctcgtgc
     1081 ttaatcgtat aatattttta tgtttaaaca atagggggaa tcttatgatt gaaaaattag
     1141 taaccttttt aaatgaggtt gtttggagta agccattagt ttatggtttg ctaattactg
     1201 gtgtgctatt tacattgcgt atgcgatttt ttcaagttag acattttaaa gaaatgattc
     1261 gattaatgtt tcaaggagag aagtctccta atggtatttc aagttttcaa gcgatagcca
     1321 tgtctttagc aggcagggtt ggtacaggta atattgtcgg tgtatctact gcaatattta
     1381 taggaggacc tggtgcagta ttttggatgt ggattactgc gtttttaggt gcaagtagtg
     1441 cttttattga atctacactt ggtcaaatat tcaagagagt tgaaaataat gaataccgtg
     1501 gtggaccagc gtattatata gaatatggta ttggtggtaa atttggtaaa atttacggaa
     1561 ttatctttgc tattgttacg attatctcag taggtctatt gcttcctggt gtgcaatcta
     1621 acgctatagc aagttctatg cacaatgcga ttcatgttcc acaatggtta atgggtggta
     1681 ttgttgtagt tattttggga ttaattattt ttggtggtgt acgtagtatt gccaatgttg
     1741 caacagccgt tgtaccattt atggcaatta tttacatact aatggctgtc attatcattt
     1801 gtatcaatat acaagaagtg ccagcgttat ttgcattaat tttcaaatca gcatttggat
     1861 tacaatctgc ttttggtggt atcgttggcg caatgataga gattggtgtt aaacgtggat
     1921 tatactcaaa tgaggctggt caaggtacag gtccacacgc agcagcggca gcagaagtat
     1981 cacatccaag taaacaaggt ctagtacaag cattttcagt ttatattgac acattatttg
     2041 tatgtactgc aactgctctg attatactta tttctggtac atataatgtg actgatggta
     2101 cggttaatgc gaatggcaca ccgcatttaa ttaaagacgg tgggatttat gttgaaaatg
     2161 caacaggtaa agattattca ggtactgcga tgtatgcaca agccggcatt gataaagcat
     2221 tccatggcag tggttatcaa tttgatccta cattctctgg cgtaggttcg tactttattg
     2281 catttgcttt attcttcttt gcatttacta caattttgtc gtactactac attacagaaa
     2341 caaatgttgc ttatttaacg cgtaatcaaa ataatcaagt ttcatcggta tttattaata
     2401 ttgcacgtgt gattatttta ttcgctacat tttacggtgc agttaaaaca gctgatgtag
     2461 catgggcatt cggtgattta ggtgtaggtc taatggcttg gttaaatatc attgcgattt
     2521 ggattttaca taagcctgcc gtaaatgctt taaaagatta tgaaattcaa aagaaacgat
     2581 taggtagcgg ttataatgca gtttatcaac ctgatacgaa gaaattacca aatgctattt
     2641 tttggttgaa gacgtatcca gaacgtttaa aacaagcacg tgccaaaaag taatctactt
     2701 ttgtttatag tatatgtagt aatcatttga taaaaaagaa aagtattgag aattttaggt
     2761 gctcagaaat ttgaatttta aaatatagtg tctcttggta caataataat acaattacta
     2821 gggggcactt ttttatgttg gaatttaaag ctggaa
//
LOCUS       DAPIDM010000198         2829 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000198 DAPIDM010000000
VERSION     DAPIDM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.203"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..2555
                     /gene="adhE"
                     /locus_tag="RJE42_002572"
                     /gene_synonym="adhC"
     CDS             <1..2555
                     /gene="adhE"
                     /locus_tag="RJE42_002572"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV5877960.1"
                     /translation="MIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAK
                     LAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICG
                     VTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKRAAEVVLEAAMKAGAPKDII
                     QWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTA
                     HIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLE
                     NAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLIIAEISGVGSDYPLSREKLSP
                     VLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRMKACRVLVNTPS
                     AVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQ
                     IYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQRTEQPQIKIFSEVEP
                     NPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWMFFEHPETSFFGAKQKFLDI
                     GKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVAIID
                     PQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKG
                     DKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNA
                     KDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVESLAKAVYELGQSVGIEMN
                     LKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ"
BASE COUNT         1040 a          449 c          540 g          800 t
ORIGIN      
        1 ttatgattga tgctctagct gacaaaggga aaaaagcatt agaagcatta tctaaaaagt
       61 cacaagaaga aattgatcat attgttcatc aaatgagttt agcagctgtt gatcaacata
      121 tggtgctagc aaaattagca catgaagaaa ctggaagagg tatatacgaa gataaagcga
      181 ttaaaaattt atacgcttct gaatatatat ggaattcaat aaaagacaat aagacagtag
      241 ggattattgg tgaagataaa gaaaaaggat taacgtatgt agcggaacca attggtgtta
      301 tttgtggtgt tacgccaaca acaaatccta cgtcgacaac tatttttaaa gcgatgattg
      361 caattaagac aggaaatcca atcatttttg cattccatcc aagtgcacaa gaatcgtcga
      421 agcgtgcagc agaagttgta ttagaagcgg caatgaaggc aggtgcacct aaagatatta
      481 ttcaatggat tgaagtgcct tctatcgaag caacaaaaca attaatgaat cacaaaggta
      541 ttgcattagt tctagcaaca ggtggttcgg gcatggttaa gtctgcatat tcaactggta
      601 aaccggcatt aggtgtggga ccaggtaacg tgccgtctta cattgaaaaa acagcacaca
      661 ttaagcgtgc agtaaatgat atcattggat caaaaacatt tgataatggt atgatttgtg
      721 cttctgaaca agttgtagtc attgataaag aaatttataa agacgttact aatgaattta
      781 aagcacacca agcatatttt gttaaaaaag atgaattaca acgcttagaa aatgcaatta
      841 tgaatgaaca aaaaacaggt attaagcctg atattgtcgg taaatctgca gttgaaatag
      901 ctgaattagc aggtatacct gtccccgaaa atacaaaact tatcatagct gaaattagcg
      961 gtgtaggttc agactatccg ttatctcgtg aaaaattatc tccagtatta gccttagtaa
     1021 aagcccaatc tacaaaacaa gcatttcaaa tttgtgaaga cacactacat tttggtggat
     1081 taggacacac agccgttatc catacagaag atgaaacatt acaaaaagat tttggactaa
     1141 gaatgaaagc ttgtcgtgta cttgtaaata caccatcagc ggttggaggt attggtgata
     1201 tgtataacga attgattccg tctttaacat taggttgtgg ttcatacggt agaaactcaa
     1261 tttcacataa tgttagtgcg acagatttat taaacattaa aacaattgct aaacgacgta
     1321 ataatactca aattttcaag gtgcctgctc aaatttattt tgaagaaaat gcaatcatga
     1381 gtctaacaac aatggacaag attgaaaaag tgatgattgt ctgtgaccct ggtatggtcg
     1441 aattcggtta tacaaaaaca gttgagaatg tattaagaca aagaacggaa cagcctcaaa
     1501 ttaaaatatt tagcgaagtc gaaccgaacc catcaacgaa tacagtatat aaaggtctgg
     1561 aaatgatggt tgatttccaa ccggatacaa tcattgcact tggtggtggt tcagcgatgg
     1621 atgctgcaaa agcaatgtgg atgttctttg aacaccctga gacatcattc ttcggtgcta
     1681 aacaaaagtt cctagacatc ggtaaacgta cttataaaat aggcatgcct gaaaatgcga
     1741 cgttcatttg tatccctacg acatcaggta caggttcaga agtaacacca tttgcagtta
     1801 tcacagatag tgaaacaaat gtaaaatatc cgttggctga ttttgcttta acacctgacg
     1861 ttgcaattat tgaccctcaa tttgtgatga gtgtgccaaa aagcgttaca gcagatacag
     1921 gaatggatgt actaacgcat gcaatggaat catatgtatc tgtaatggct tcagattaca
     1981 caagaggttt gagtctacaa gcgattaaat tgacgttcga atatttaaaa tcatctgttg
     2041 aaaaaggtga taaagtttca agagagaaaa tgcataacgc atcaactttg gctggtatgg
     2101 catttgcaaa tgcattctta ggcattgcac actcaattgc gcataaaatt ggtggcgaat
     2161 atggtattcc gcatggtaga gcgaatgcga tattactacc gcatattatc cgttataatg
     2221 ccaaagaccc gcaaaaacat gcattattcc ctaaatatga gttcttcaga gcagatacag
     2281 attatgcaga tattgccaaa ttcttaggat taaaaggtaa tacgacagaa gcactcgtag
     2341 aatcattagc taaagctgtc tacgaattag gtcaatcagt cggaattgaa atgaatttga
     2401 aatcacaagg tgtgtctgaa gaagaattaa atgagtcaat tgatagaatg gcagagcttg
     2461 catttgaaga tcaatgtaca actgctaatc ctaaagaagc actaatcagt gaaatcaaag
     2521 atatcattca aacatcatat gattataagc aataatctat ctgataataa tcatctaact
     2581 cacctgaaat tacaaaagta aaaaatgcca cataaacttt aagtcgataa tcattatacg
     2641 gttatcggct tttatttatt gccaaatctt cagagagata caacctagac aaacattttt
     2701 taaataaaga aaatattaag attggtacac ttttcgcaaa ctattaccac tttagagtat
     2761 aattattttt aatttcatat aaataaaagg gcgaaaataa tgcggtttaa aagtaattaa
     2821 ttgtttaaa
//
LOCUS       DAPIDM010000199         2761 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000199 DAPIDM010000000
VERSION     DAPIDM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.204"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(23..238)
                     /locus_tag="RJE42_002573"
     CDS             complement(23..238)
                     /locus_tag="RJE42_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDV5877961.1"
                     /translation="MKVNKAIYVNVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADRDGYIIFPKN"
     gene            403..954
                     /locus_tag="RJE42_002574"
     CDS             403..954
                     /locus_tag="RJE42_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV5877962.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CIDATYRTFYQHVLVYEIENKVAGCIISYSGENELKYEKAWEQLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(1005..1943)
                     /locus_tag="RJE42_002575"
     CDS             complement(1005..1943)
                     /locus_tag="RJE42_002575"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV5877963.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            2094..2171
                     /locus_tag="RJE42_002576"
     CDS             2094..2171
                     /locus_tag="RJE42_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877964.1"
                     /translation="MKKDEERSEMDGYGRIRGRYCQRDI"
BASE COUNT         1049 a          399 c          483 g          830 t
ORIGIN      
        1 ttttaaacgt cattgtcaca atttaatttt tagggaatat aatataaccg tctctgtctg
       61 cttttttagt aaaaatgaca aaaatggcat gtattattga gatgatggta gggatacctg
      121 tccagaaaaa taataagtga aaaagacctt gtccaaattt atcagcataa aatttatgaa
      181 tacctaaacc tccaagaaat aatgcaacat taacataaat ggctttattg actttcattt
      241 gtaaatcctc cttaattata attctactta aattcgttgt gaaaaccaat ctttctaact
      301 ttagaatttt caaactttct aaaattataa gtatatcttt ttaaaataag ctagaatttc
      361 tatataataa atgttaataa cgtaaaaggg aatgatgaca tagtgatacg tcaagcacgt
      421 ccagaggacc gatttgatat tgcgaagtta gtttatatgg tttgggatga tatggaatta
      481 gaattggtaa agcatctacc taaagacatg gtattagatg caattgaaaa aagctgtatt
      541 gatgcaacat atagaacttt ttatcagcat gttttagttt atgaaataga aaataaagta
      601 gcaggttgta ttattagcta tagcggtgaa aatgaattga aatatgaaaa agcatgggaa
      661 caacttgact tgccagaaga aataaaacaa tatggcacgc cattacctgt aaaagaagct
      721 aaagacgatg agtattatat agaaacaatt gcgacatttg cagcatatag aggtagaggc
      781 atcgcgacaa agttattaac gtcattactt gaatcaaata cacatgttaa atggagtttg
      841 aattgcgata ttaataatga agcagcatta aagttatata aaaaagtagg ctttatatct
      901 gatggacaga ttgaattata caagcacatg tatcatcatt taattgttaa ataaaaaact
      961 cgacagttcg atgtaagtcg attgccgagt agtatcattt tatattaaat gcctgcaaat
     1021 aatgcactaa tataaatacc taatgcatat aataaaccga aaaatgtatt tgttttacca
     1081 gcagcagcca ttgctggcat cattgtaggc ggtgtatcat tcttcttgaa acgtctgata
     1141 actttaacag gcattgggaa tgataacaac gcaagtaagt aaaataatga gccaccaggt
     1201 ttaataatga tcgtaagtac aataaaggca taagcgataa agtacatgat tgccataaat
     1261 gttaaagaag catttttacc taatagaatg ggtaaagttt tacgaccact tgctttatct
     1321 ttgacacggt cgcgaatatt gttagccata ttaattaaac cgatagtgat tactataggt
     1381 acacttaacc aaattacata gctttgaata ttgccagttt gaataaagaa tgcaataacg
     1441 ataataaaca tacccataaa tacgcctgag aataattcac cgaaaggcgt ccatgaaata
     1501 gggaaagggc cacctgtata taggtaacca acagccatac atactaatcc aactggtaat
     1561 aaccaaaatg aagagttagc agctaaaaac aaacctaata ttgctgctaa gatgtaaaat
     1621 gcaatggcta atcgtagcac aagctctggg ctcataccgt tgcgtacaat tgcaccacca
     1681 atgcctacag attcatgatc atcaaggcct tttttataat catagtattc gttaaacata
     1741 ttagttgccg cttgaataag taagcatgct agtaacatgg caatgaatag gctgatttta
     1801 atatgatctt cgctaccaag aaaatatatt ttagatgctg ctgtaccaac taaaacgggt
     1861 actacggaag cagttaatgt atgaggacgc attaaatgcc aatatttctt aactgtagaa
     1921 tattgctgat attgattact cattgaatac aaaacctctt taaatcatat tttttataac
     1981 aaagatattt taatagaaag tagcataaag gtcaattgac gtgaaaaatt gcaagataag
     2041 tttaagcaat tcttgtaaca caattatcca aaaatgatac aatataataa tgagtgaaaa
     2101 aagacgaaga aagaagtgaa atggatggct acgggcgtat tagaggacga tattgtcaaa
     2161 gagatatatg acagctcaaa ggaatgggtt tcagttgaag tgaaattatc acagtcacta
     2221 gacccgagca cattatttca tctcactgac aatgaggcag gagatcgctt ttatatgcgt
     2281 ttgaatgata atcgaacgtc atattttggc tacaaagcaa ttcaattatt caaaaataat
     2341 tctaaaaata aacaatctat ttttaaagac tgggaaaaat taaaacataa catcacattt
     2401 atacatccgc aatctgagaa acatcatctt cgagttgttg gagggttcca attttcaagt
     2461 cataaatcag atgatgaatg gcgagagttt ggactaaatc attttgtatt acccgaagtt
     2521 ttaatttcaa ctgataataa tgggacattt ttaacttata cagttaaaag ggaaagtttt
     2581 actgttgagg cattgaacga tttaatggat ttgttcaaca atacatcgga tatatatgtg
     2641 gacgagcaaa ttggggaaat tactagaaat gaagatattt ataaaggtga ctggcgtcaa
     2701 cttgtagtag aagcaataga atctattaat aatgaagaaa aaattgtact agcacgtaga
     2761 c
//
LOCUS       DAPIDM010000200         2745 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000200 DAPIDM010000000
VERSION     DAPIDM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.205"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            373..813
                     /locus_tag="RJE42_002577"
     CDS             373..813
                     /locus_tag="RJE42_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDV5877965.1"
                     /translation="MNTKYFLAVGAVASVLILGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNSETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1154..1627
                     /locus_tag="RJE42_002578"
     CDS             1154..1627
                     /locus_tag="RJE42_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV5877966.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIIVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1602..2288
                     /locus_tag="RJE42_002579"
     CDS             1602..2288
                     /locus_tag="RJE42_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV5877967.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2288..>2745
                     /gene="saeS"
                     /locus_tag="RJE42_002580"
     CDS             2288..>2745
                     /gene="saeS"
                     /locus_tag="RJE42_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDV5877968.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGI"
BASE COUNT         1009 a          415 c          411 g          910 t
ORIGIN      
        1 tgatggtttt aacaccatat tggtacttgt atttaatcgt ctatcttatt ttcttgttgt
       61 ttttattttg gcaaaaggtt tataaatttt aataccaaaa ctaaaaaaca cttctgatat
      121 tcttaattcg ctatatcaga agtgtttttt agcgataagt agttcagaca aatattttct
      181 tacttaaaaa aaccgccctc ctcttatttt gactcctatt tatttaaatc agacaattat
      241 tttcattttc aaattattct ttcttcaata ttagttaagc gatatttaaa cgaagttaag
      301 aattagttaa tggcatatta tttgccttca ttttaaactt aacttatcaa attgaagaaa
      361 tgaggagtta gcatgaatac aaaatatttt ttagcagttg gtgctgttgc ctctgtatta
      421 attttaggcg cttgtggtaa ttctaattca caagatcaag gtaacaaaac tgaacaaaaa
      481 acaaagtcag aagatagcaa tgttaaaact gataaaacaa aacacctaac tggtacattc
      541 agttctaaaa acagtgaaac tgttgaaggt aaagctgaga ttaaaaatgg taaattaatg
      601 cttactaact acaaatcatc aaaaggtcca gatttatacg tctatctaac aaaaaatggc
      661 gacattaaaa acggtaaaga aatcgcaatg gttgactacg ataaagaaaa acaaacattt
      721 gatcttaaaa atgtagattt aagcaaatat gatgaagtaa caatctattg taaaaaagct
      781 cacgtcatct tcggcggcgc taaattaaaa taatcaattt ctgagttaaa cttttattta
      841 caacatacta ttactataca ttacaaattt taaaaatata cattacactc attactcaat
      901 ggaagcgtat gatttcacag cccccctagc ttgtagaaat catactttcc ttttttcaat
      961 atatatacaa ctattaaatt ccataagatt gtagagcaca taagtaaatt tttttagagc
     1021 ttgaggtttg tttagcttaa gcagcccatg agctcaaaca cttcctgttc acataacact
     1081 acaaatcgca ttatgttgct taatcttatg tttatataaa ttacacacaa taaatagaaa
     1141 gaatgtgaac atcatgaata aattattgct actcgttaca tttatcattc gtgtgggttc
     1201 aggtattgtt atgttaatgc aaggctacga aaaattaacg ggcggattta cgctgaaagg
     1261 tctagtacca gtcatagcta acaatactga ttcaccagag tggtataagt ggtttttcgc
     1321 aaatatagtt gcacatacga cgtcattatt tgatattatt gttccacttg gagagattgc
     1381 aattggatta ggtttaattt ttggagtttt tgcatatgct gctagtttct ttggagcctt
     1441 tgttatgata aattatatct tagcagatat gatatttacg tatcctcttc aattaacttt
     1501 ctttatcctt ttactaatga gtcactcatt gttaaaacag atttcactta aagaaatcat
     1561 taattacttt agaggtcgta agaacagagg tgaaaaaata gatgacccac ttactgatcg
     1621 tggatgatga acaagacatt gtagacattt gtcaaaccta ttttgaatat gaaggttaca
     1681 aagtaacaac gacaactagc ggtaaagaag caatttcttt actatcaaat gatattgata
     1741 tcatggtact tgatatcatg atgccagaag ttaatggtta cgacattgtc aaagaaatga
     1801 aaaggcaaaa attagatatc ccctttatct atttaactgc caaaacacaa gaacatgata
     1861 ccatttacgc cttaacttta ggtgcagatg actatgtcaa aaaaccattt agtccaaggg
     1921 aactcgtttt acgtattaat aatttactta caagaatgaa gaaataccat catcaaccag
     1981 ttgaacaact gtcgtttgat gaattaacac ttattaactt aagtaaagtt gtgactgtaa
     2041 atggtcacga agtccctatg cgtattaagg aatttgagtt attgtggtat ttagcttcta
     2101 gagaaaatga agttatttct aaatcagaat tacttgaaaa agtttgggga tatgactatt
     2161 acgaagatgc taataccgtg aatgtccata tacaccgtat tagagaaaaa ttagaaaaag
     2221 agagctttac aacatatacc atcacaactg tatggggatt aggatataaa tttgaaagga
     2281 gccgataatg gtgttatcaa ttagaagtca aatcattatt ggcgtcgtat cgagtatact
     2341 attaacttca actattttag caattgcata tattttaatg tggtttaacg gccatatgac
     2401 actaactttg accttaacga caataattac aagctgttta accttattaa tatgtagtat
     2461 ttttattaat ccacttatac aaaaaattaa gcagtttaat ataaaaacta agcaatttgc
     2521 taacggaaat tacgcaagca atgataaaac gtttaattca ccaaaagaaa tttatgaatt
     2581 aaatcaatct tttaataaaa tggcttctga aattacgcaa caaatgaatc aaattaaatc
     2641 cgaacaacaa gaaaaaacag aactgattca aaacttagcc catgatttaa aaacaccttt
     2701 agcaagcatt atttcatatt ctgaaggact acgtgatggt ataat
//
LOCUS       DAPIDM010000201         2726 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000201 DAPIDM010000000
VERSION     DAPIDM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.206"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..552
                     /locus_tag="RJE42_002581"
     CDS             <1..552
                     /locus_tag="RJE42_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDV5877969.1"
                     /translation="AIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFF
                     KENEKKYQYTGFTNEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDKEFK
                     EGMQRARKELNYTANTDAVSTLFSTKKNFTKDNTVDDVIELSNKIKDEPNMPQKSQVT
                     IQLGKPSINTKKPFYDDINPIEG"
     gene            1007..1939
                     /locus_tag="RJE42_002582"
     CDS             1007..1939
                     /locus_tag="RJE42_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDV5877970.1"
                     /translation="MLKKAKLTLIATVLLSGCSIMENESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKFAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGTVLSGFEYRAHKEELDNLTEVLK
                     EYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDKSFRD
                     GMKNSKKQLKDKSRPYIVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPRDLNVSL
                     QISNKYINTKRPNYSKKDVIEVGVFNHEKTNTND"
     regulatory      2147..2281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2343..>2726
                     /locus_tag="RJE42_002583"
     CDS             2343..>2726
                     /locus_tag="RJE42_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV5877971.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIISTIVMTIVLSILNYFVLL"
BASE COUNT         1076 a          361 c          480 g          809 t
ORIGIN      
        1 gccatccatc aaaaaggttc aatgcgtagt aatgacaacg gtgatgatat gagtatgatg
       61 gtgggtacag tgctgagtgg ctttgaatat cgagcgcaaa aagaaaagta tgataattta
      121 tataaatttt tcaaagaaaa tgaaaagaag tatcaatata caggttttac aaacgaggca
      181 attaacaaga cacaaaacgt cgggtatcaa aatgaatact tttatattac atatttatca
      241 agaaacttaa aagaatatcg taaatattat gaaccattga ttcataaaaa tgataaagaa
      301 tttaaagaag gtatgcaacg agctagaaaa gagctaaatt atactgcaaa tactgatgca
      361 gtttctacac tcttttctac taagaaaaac tttactaaag ataatacagt agatgatgtg
      421 attgaattga gtaataaaat aaaagatgaa cctaatatgc ctcaaaaatc tcaagttact
      481 attcaattag gaaaacctag tataaatact aaaaagccgt tttatgacga tataaatcca
      541 attgaaggat aagtgaatat aaagtgaata aaataaatga tggagatata gcagaattga
      601 acggttattg ggtttataaa gatataaatg aatgatagat tttaaagtaa atggaaaacg
      661 gttcaaacaa atagaaaatc ataatatgaa aggtacacct taatatagaa atatacgagt
      721 ttcttgatga tcaggggaat ctaactggtc aacagataat aaactattaa ggaacttcaa
      781 atgaggcagt aaatcctaat aacccattga aatctttagg taatggagat gattggcttc
      841 aaaatgctaa atcaacggat aattaaataa aaatcagaca ttgtttaacg caagtagatg
      901 tatttgaaga tttatacaga gagaaactat aaggcgcaaa taaattaaga gagtataatt
      961 atttgcaaaa tgaaagtttt aaaagcgcat aaatgggtga tgagatatgc ttaaaaaagc
     1021 aaaattaacc ttaatagcaa cagttctgct atcaggatgt tccataatgg aaaacgaatc
     1081 gaataaagaa acaaaatcag taccagaaga aatggaagct tccaaatatg taggacaagg
     1141 cttccaacca cctgcagaaa aagatgcgat tgaatttgcg aagaagcata aagataaatt
     1201 tgctaagcga ggcgaacaat tttttatgga taacttcggt ctaaaagtta aagctacaaa
     1261 tgttataggt agtggcgacg gtgtagaagt attcgtgcat tgtgatgacc acgacatcgt
     1321 atttaatgca agtattccat tcgataaatc aattattgac agtgatagtt cattaagaag
     1381 taaggataaa ggcgatgata tgagtacttt agttggtaca gtgttgagtg gctttgaata
     1441 tcgagcacat aaggaagagt tggataattt aacagaagtt ttaaaagaat ataaaagtaa
     1501 atataaatat actggttata ctgaaaatgc aattatgaaa acgcagaata gtggttttag
     1561 aaatgagtat tattatttga ctgcaattcc atatacttta gatgaatata aaaagtattt
     1621 tcaaccctta atcaaagagg atgataagtc ttttagggac ggtatgaaaa actcaaaaaa
     1681 acaacttaaa gataaatctc gtccatatat tgttacaact ttatttagta ctaaagataa
     1741 ttttactaaa gataatacaa tagacgagat gattgatttt tctgaagtac taaaaaagaa
     1801 aaagaatatt cctcgtgatt tgaatgtttc tcttcaaatt tctaataaat atataaatac
     1861 aaaaagacct aactatagta agaaggatgt aatagaagta ggtgtattta atcatgaaaa
     1921 aactaacacg aatgattaaa ctaataacaa taaaaaggta ggttcaaaaa agccgtccca
     1981 aaatccatct caaaaaacaa tcaagcaaac aacattccaa acattgctcg caaatcacca
     2041 atgtatcact ctccaattac gtaactatga tctaattaaa gcacagttat tgaggttttg
     2101 tgatatatag tataaaatta atgagaatta aatttaataa tgtaaaattc atcttcgggg
     2161 tcgggtgtaa ttcccaaccg gcagtaaata aagcctgcga cctgctagta tgtatcatat
     2221 tagtggctga tctagtgaga ttctagagcc gacagtatag tctggatggg agaagatgga
     2281 ggttttttgt tgtgcaataa tcctcctatt cttacgagat gaatggaagg agaaaattga
     2341 atatgcaaca aaataaacgt cttatcacaa taagtatgtt gagcgcgatt gcgtttgtgt
     2401 taacttttat caagtttcct ataccatttt tgccaccata cttaacttta gattttagtg
     2461 atgtaccgtc actactagct acatttacgt ttggaccagt tgccggtatc atagttgcac
     2521 tggttaaaaa tttattgaac tacttattta gtatgggcga tccagttgga ccatttgcta
     2581 actttttagc aggcgcaagt ttcttattaa ctgcttacgc catctataaa aataaacgtt
     2641 caacaaaatc tttgattact ggattaatca tttcaacaat cgttatgact atcgtgttga
     2701 gtatcttgaa ctatttcgtt ctatta
//
LOCUS       DAPIDM010000202         2655 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000202 DAPIDM010000000
VERSION     DAPIDM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.207"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..595
                     /locus_tag="RJE42_002584"
     CDS             <1..595
                     /locus_tag="RJE42_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDV5877972.1"
                     /translation="FKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKK
                     HIHQQNRKKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFH
                     EQILASLSKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAA
                     IEEYRHHLEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(685..777)
                     /locus_tag="RJE42_002585"
                     /pseudo
     CDS             complement(685..777)
                     /locus_tag="RJE42_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(786..2522)
                     /locus_tag="RJE42_002586"
     CDS             complement(786..2522)
                     /locus_tag="RJE42_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDV5877973.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPVEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFLFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLIRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHHELIAKQ
                     GAYEHLYSIQNL"
BASE COUNT          932 a          474 c          361 g          888 t
ORIGIN      
        1 gtttaaatta gtattaaatg atacgacgga gtttaataat attaaacaag atagtcataa
       61 tttaaaacaa cgtgtcgaaa agttagagaa aatttatgat tactatagcg aagaacgtcc
      121 tattacaaaa aaacacattc accaacagaa tagaaagaaa atacttttca gagaagtagt
      181 tcagacgact agacaagctt atgaagtatt aaacaaattg tcgcgttatc aaaacgactt
      241 atatttactt aataacaatt tcctattaca aatcaaatta gatttagatt cattaactgc
      301 ttttcatgag caaattttag ctagcctttc taaaaaagca cgttataatg tgacacatgt
      361 tgattatgag ttagataatc cgcagaaaaa agatttattg tctacatttc agcatgagtt
      421 aataaatcac ccttatcaaa cggaatattc gtttgccaat gttatgcaaa ttgttgcagc
      481 catcgaagag tatcgacacc atttagagca tttagaccgc ataagaatta gctttttcac
      541 atatcataga tctgatgcag acattgaaat tgttgaagaa gactttgatt tataacatta
      601 aaacaatttc tgtaattaat accacattga gataaagtaa tattgaatct cgaaattaaa
      661 tttaaaaaag cagtaagatt attttcaatg aagaaaatct cttactgctt tttctatgtt
      721 ttgaccccac catcctgtta caaatcttcc ctacaaaatt gtactgaagt caaacaacta
      781 atactttata aattttgaat gctatataaa tgctcgtaag caccttgttt tgcaatcaat
      841 tcatgatgcg tacctgtttc aacaatatgt ccattttcaa ttacgacaat tttgtcagca
      901 tgtgtaatag tggacaagcg atgcgctacg ataagtgtcg ttcgatcttt actcaacaca
      961 tctaatgctt cttgaataat ggattcactt tctaaatcaa gtgcacttgt tgcttcatcc
     1021 aggataagaa ttggcggatt atttaaaaat attctagcaa tcgataacct ttgtttttga
     1081 ccacctgata atttaacacc tcgttcacct acttcagtgt catatccctg tggcaagttc
     1141 atgataaagt catgtgcatt agccattttc gccgcttcaa ctacttcttc atctgttgct
     1201 gttggacgac caagtaaaat attttcctta actgtatcag agaataaaat attatcctgt
     1261 tgcaccaatc ctatttgatt tcttaaactt cccgttaaaa aatctttaat gttgtgacca
     1321 tctattaaaa tttgcccaga agttacatca taaaatctcg gtattaagtt aattaatgtt
     1381 gatttaccac caccactcat acctacgaaa gcaactgttt ctcctttttc aatactcaaa
     1441 ttaatatctt ttaaaattgg agcttcgtta tcgttatatt gaaaactaac atgatcaata
     1501 tcaatacgac cttgtttaat ttcaataggt tgagcaccaa caccattttt aatgtcataa
     1561 tcttcgtcaa ttaattggaa tacacgatcc attgaagcaa aactttgcgt taaagttgta
     1621 aatgatgcga ctaaacgacg taaaggcccg aacaataact ccaagtatcc aacaaatgct
     1681 gcaagtgtac ctactgtgat tgatccagaa atagcaagat atgcaccaac accgatgaca
     1741 ataattggtc caatatctgt aactgtatta attgcggcaa aggaataggc attccatctt
     1801 gtatgtttca acgcacgtat taaaaaatta gtgttctttt tatcaaagtt tttcgcttca
     1861 ttgtcttcaa tcgcaaaact tttaacgact gaaatacctt gaacacgttc atgcaagaat
     1921 ccttgaacct cagctaatgc ttgagatctt tcacgcgtca attttcttaa tcttccaaag
     1981 aaaacgtaca ccgttaaaat gtaaaatagg aagataaaca gtgctgctaa agtcaatttc
     2041 acatctaaaa agaacattat ggatagtgca ataataattg ttatacaatc taaccaaata
     2101 ttcattaacc ctgttaaaat gaaatctttt gtttgttcaa catcattaat tactctagat
     2161 attacttgac ctacttgatt attagcataa aatctcgcac ttaaagcttg taaatggttg
     2221 tataactttt tacgtatatc atacaatatt ttattacttg tccattgcgc caaatattga
     2281 cgtataaatt caactggtgg tctcactatt acaaaaataa ataatgcgat accaatggca
     2341 atagttaaat gatgaacttt ttcatcagtc gttaatgcgt ggttattaat cacgccatct
     2401 attgcatatt taattaaaag tggtataagc attggtatac caaacttaat tatcccaaca
     2461 ataatcgttg caaaaatacg atatttgtat ggcttaacaa attgcaaata tcgtttaatc
     2521 atacaatccc ccctaatcta ttgccctatc ctattcataa gcataaaaat gaatagaggt
     2581 tggatacata atttgtagat gtaaattctt cttacaattt acatttttaa aattaagata
     2641 tctcaacctc tgtca
//
LOCUS       DAPIDM010000203         2651 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000203 DAPIDM010000000
VERSION     DAPIDM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.208"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..562
                     /locus_tag="RJE42_002587"
     CDS             <1..562
                     /locus_tag="RJE42_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877974.1"
                     /translation="LVLMAFFIKNSSKKSAENKIVYLIQTFIKETENKLAYSELELSV
                     IFDYINNGDNNIFKSTNLNSKNLLSKLKGIAWDLFHIRTSEDQIALRNTNSKEVFLHS
                     IFTADKGLSNVINNYSISRMLAHEEKLYVYRDNNIFKKLSKDKATKIQELLEKDRNSR
                     ISNVREAKYNIQENIDIYEKYINEMI"
     gene            816..>2651
                     /locus_tag="RJE42_002588"
     CDS             816..>2651
                     /locus_tag="RJE42_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV5877975.1"
                     /translation="MIKKISLQNIATYRNYVEIKPKKINFIYGSNGSGKTTISNLIGR
                     FNKSDDCVIETKKNSNSSILVYNKKFVEKNFSQSDIGLKGIFTLGENSINLQDNLNEL
                     RRKIQVNEENIYIKEKTIRGFEEEIEERTNIIKDKIWNHQKEISKDFPEAFVGYKNNK
                     QKFLNQCIKVYEENILEKSEEILTNSLEKLKENYNISFSDDIKLYEVFNVNDTEKILE
                     IEKTNLIEKVIVGGNDSLIGQFIQSLKNSDWVKQGLDYLDYSNHTCPFCNQSISEKLE
                     QEIKDYFNEEYEQDLKKIKEIKQVYSSCFEKIESNMLFILRKSIPYLNTKLLSKEFEV
                     LEKQYEVNQKQLKEKLRNPSQKLKIKSLVPKIENINEIISSLNESIETNNNIIRNKER
                     EQNKFKKNLWIYIVNNMKQDLKEYVNFKNGKFKAIANIRRQISGLKNENKKTNKEIGE
                     IETNITSVEPTVKNINEILNKFDFKGFKLQENSNAKGTYLIVREDGSNANETMSEGEY
                     NFITFLYFYYLVYGSHESTSLTSNKIVIIDDPISSLDSNVLFIVSTLVKNLINDCRNN
                     KNGIQQIFNLTHNIYFHKEITFLGSRERFSPNEVMYGIIRKKDNIS"
BASE COUNT         1192 a          231 c          399 g          829 t
ORIGIN      
        1 attagtttta atggcatttt ttataaaaaa tagcagtaaa aaatcagcag aaaataaaat
       61 tgtttacttg attcaaactt ttattaaaga aactgaaaat aaattagctt actcagagtt
      121 agagttaagt gttatttttg attacataaa taatggtgat aataatatat ttaaatctac
      181 caatttgaat agtaaaaatt tattgagtaa attaaaagga atagcttggg atttatttca
      241 tattagaacc tcggaagatc aaatcgctct aagaaatacc aatagcaaag aagttttttt
      301 acattcaatt tttacagcag ataaaggctt gagtaatgtg ataaataact attcaataag
      361 taggatgcta gcacatgaag aaaaattata tgtatataga gataataata tatttaaaaa
      421 attatcaaaa gataaagcta ctaaaataca agaattgtta gaaaaagata gaaattctag
      481 aatttctaat gtaagagaag caaagtacaa tatacaagaa aatattgata tatatgaaaa
      541 atatattaat gaaatgattt aaaaatagaa ttattagagt cgattataga gaagataaag
      601 tattaagcca ttataacgat ttgttacttt agtaatatca ttgataaggg gaggcttatc
      661 ataaatgatg aggttaaata ctaaaaataa tattaaatac attagtaaca tctcagacat
      721 aattttgatt attagaaata aatttaaagc ccacagttag cagttgataa atatagtgtc
      781 tttagtaatt aaaataaggg attggggtat ttagaatgat taaaaaaatt tcacttcaaa
      841 acattgcgac ttatagaaat tatgtagaaa taaaacctaa aaaaataaat tttatttatg
      901 gtagtaatgg cagtggtaaa actactattt cgaatttgat aggaagattt aataagtccg
      961 acgattgtgt tattgaaact aaaaaaaata gtaactcaag tattttggtt tataacaaaa
     1021 aatttgttga aaaaaacttt agtcagtctg atatcggtct aaagggaatt ttcactttag
     1081 gagaaaattc cataaattta caagataatt taaatgaatt aagaagaaaa atacaggtta
     1141 atgaagaaaa tatatatatt aaagaaaaaa ctataagagg ctttgaagaa gaaattgaag
     1201 aaagaacaaa tataattaaa gataaaattt ggaatcatca aaaagagatt agtaaagact
     1261 ttccagaagc ctttgttggc tataagaata acaaacaaaa attcttaaat cagtgtataa
     1321 aagtttatga agaaaatatt ttagaaaaat cagaggaaat cttaacaaat agtttagaaa
     1381 aattaaaaga aaattataat ataagttttt ctgatgatat taaattatac gaagtgttta
     1441 atgttaatga tacggaaaag attctggaaa tagaaaaaac gaatttgata gaaaaagtta
     1501 ttgttggagg caatgatagt ctaatcggac aatttataca gtcacttaaa aatagtgatt
     1561 gggttaaaca aggtttggat tatttggact actctaatca tacttgccca ttttgtaatc
     1621 aaagtatttc tgaaaaacta gaacaagaaa taaaagacta ctttaacgaa gagtatgaac
     1681 aagatttaaa aaaaataaaa gaaattaaac aagtatatag cagttgtttt gaaaagattg
     1741 aaagcaatat gttgtttata ttgaggaagt caatacccta tcttaataca aaattactta
     1801 gtaaagagtt tgaagtactt gagaaacaat atgaagttaa tcaaaaacaa ttgaaagaaa
     1861 aactgagaaa tccatcacag aaattgaaaa taaaaagtct ggttcctaaa attgaaaata
     1921 ttaatgaaat aattagctct ctaaatgaat cgatcgaaac taataataat attattagaa
     1981 ataaagagag agaacaaaat aaatttaaga aaaacctatg gatatatatt gtaaataata
     2041 tgaagcaaga tttaaaagag tatgttaatt ttaaaaatgg aaaatttaaa gctattgcta
     2101 atattcgacg acaaatttct ggtttaaaaa atgaaaataa aaaaactaat aaggaaatag
     2161 gagaaataga aacaaatatt actagcgtag aacctactgt gaaaaacatt aatgaaattt
     2221 taaataaatt tgatttcaaa ggatttaaac ttcaagagaa tagtaatgca aaagggacat
     2281 acctcattgt aagagaagac ggatcaaatg ccaatgaaac aatgagcgaa ggtgaatata
     2341 actttattac atttttatat ttttattact tagtttatgg tagtcacgag agtacaagtt
     2401 taacttcaaa taaaatcgtt attatagatg atccaatttc tagcttagat agtaatgtat
     2461 tatttattgt tagtacttta gttaagaatt tgataaatga ctgtagaaat aataagaatg
     2521 gaatacaaca aatattcaat cttactcata atatttactt tcataaagaa atcacttttt
     2581 taggaagtag agaaaggttt tcgcctaatg aagtaatgta tggaattatt agaaaaaagg
     2641 ataatataag t
//
LOCUS       DAPIDM010000204         2629 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000204 DAPIDM010000000
VERSION     DAPIDM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.209"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(193..276)
                     /locus_tag="RJE42_002589"
     tRNA            complement(193..276)
                     /locus_tag="RJE42_002589"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(240..242),aa:Leu,seq:caa)
     gene            complement(315..389)
                     /locus_tag="RJE42_002590"
     tRNA            complement(315..389)
                     /locus_tag="RJE42_002590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Gly,seq:tcc)
     gene            complement(493..567)
                     /locus_tag="RJE42_002591"
     tRNA            complement(493..567)
                     /locus_tag="RJE42_002591"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Gly,seq:tcc)
     gene            complement(575..648)
                     /locus_tag="RJE42_002592"
     tRNA            complement(575..648)
                     /locus_tag="RJE42_002592"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Cys,seq:gca)
     gene            complement(654..725)
                     /locus_tag="RJE42_002593"
     tRNA            complement(654..725)
                     /locus_tag="RJE42_002593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Gln,seq:ttg)
     gene            complement(737..809)
                     /locus_tag="RJE42_002594"
     tRNA            complement(737..809)
                     /locus_tag="RJE42_002594"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:His,seq:gtg)
     gene            complement(812..885)
                     /locus_tag="RJE42_002595"
     tRNA            complement(812..885)
                     /locus_tag="RJE42_002595"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(851..853),aa:Trp,seq:cca)
     gene            complement(901..981)
                     /locus_tag="RJE42_002596"
     tRNA            complement(901..981)
                     /locus_tag="RJE42_002596"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Tyr,seq:gta)
     gene            complement(987..1062)
                     /locus_tag="RJE42_002597"
     tRNA            complement(987..1062)
                     /locus_tag="RJE42_002597"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Thr,seq:tgt)
     gene            complement(1070..1142)
                     /locus_tag="RJE42_002598"
     tRNA            complement(1070..1142)
                     /locus_tag="RJE42_002598"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Phe,seq:gaa)
     gene            complement(1161..1236)
                     /locus_tag="RJE42_002599"
     tRNA            complement(1161..1236)
                     /locus_tag="RJE42_002599"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1201..1203),aa:Asp,seq:gtc)
     gene            complement(1246..1319)
                     /locus_tag="RJE42_002600"
     tRNA            complement(1246..1319)
                     /locus_tag="RJE42_002600"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1283..1285),aa:Met,seq:cat)
     gene            complement(1334..1426)
                     /locus_tag="RJE42_002601"
     tRNA            complement(1334..1426)
                     /locus_tag="RJE42_002601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1388..1390),aa:Ser,seq:tga)
     gene            complement(1465..1540)
                     /locus_tag="RJE42_002602"
     tRNA            complement(1465..1540)
                     /locus_tag="RJE42_002602"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Asp,seq:gtc)
     gene            complement(1551..1640)
                     /locus_tag="RJE42_002603"
     tRNA            complement(1551..1640)
                     /locus_tag="RJE42_002603"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1602..1604),aa:Ser,seq:tga)
     gene            complement(1650..1723)
                     /locus_tag="RJE42_002604"
     tRNA            complement(1650..1723)
                     /locus_tag="RJE42_002604"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1687..1689),aa:Ile,seq:cat)
     gene            complement(1752..1825)
                     /locus_tag="RJE42_002605"
     tRNA            complement(1752..1825)
                     /locus_tag="RJE42_002605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1789..1791),aa:Met,seq:cat)
     gene            complement(1847..1922)
                     /locus_tag="RJE42_002606"
     tRNA            complement(1847..1922)
                     /locus_tag="RJE42_002606"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1887..1889),aa:Ala,seq:tgc)
     gene            complement(1940..2013)
                     /locus_tag="RJE42_002607"
     tRNA            complement(1940..2013)
                     /locus_tag="RJE42_002607"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1977..1979),aa:Pro,seq:tgg)
     gene            complement(2023..2096)
                     /locus_tag="RJE42_002608"
     tRNA            complement(2023..2096)
                     /locus_tag="RJE42_002608"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2060..2062),aa:Arg,seq:acg)
     gene            complement(2115..2203)
                     /locus_tag="RJE42_002609"
     tRNA            complement(2115..2203)
                     /locus_tag="RJE42_002609"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2167..2169),aa:Leu,seq:taa)
     gene            complement(2214..2288)
                     /locus_tag="RJE42_002610"
     tRNA            complement(2214..2288)
                     /locus_tag="RJE42_002610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2254..2256),aa:Gly,seq:gcc)
     gene            complement(2292..2373)
                     /locus_tag="RJE42_002611"
     tRNA            complement(2292..2373)
                     /locus_tag="RJE42_002611"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2337..2339),aa:Leu,seq:tag)
     gene            complement(2377..2452)
                     /locus_tag="RJE42_002612"
     tRNA            complement(2377..2452)
                     /locus_tag="RJE42_002612"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2417..2419),aa:Lys,seq:ttt)
     gene            complement(2458..2533)
                     /locus_tag="RJE42_002613"
     tRNA            complement(2458..2533)
                     /locus_tag="RJE42_002613"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2498..2500),aa:Thr,seq:tgt)
     gene            complement(2550..2625)
                     /locus_tag="RJE42_002614"
     tRNA            complement(2550..2625)
                     /locus_tag="RJE42_002614"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2590..2592),aa:Val,seq:tac)
BASE COUNT          740 a          666 c          612 g          611 t
ORIGIN      
        1 caaaaaaaga agttaaacaa tgtatagttg cttaacttcc tatattaaac tcatcattac
       61 aatttgacat agagcctatt aaagcgtgtg ccatttgagt gccactttat ttgtattgtt
      121 taaagagaaa taaaaagaaa ccttgtttta caaggtttct agtacgttat gttatgtaaa
      181 taacagttaa tataccggtg gtcggggtcg aaccgacact ccacaagtgg aacgggattt
      241 tgagtcccgc gcgtctgcca attccgccac accggctaaa tggtaaacaa aaaacttccc
      301 tttggaagca attatggagc ggaagatagg atttacacct atacctcgtt ccgggaagga
      361 acgtgttcta aaagttgaac tactcccgca aatattaaat tatggagcgg aagataggat
      421 ttacacctat acctcattcc aggaaggaat gtattctaag agttgaaata ctcccgcatt
      481 attattaaat tatggagcgg aagataggat ttgcacctat acctcgttcc gggaaggaac
      541 gtgttctaaa agttgaacta ctcccgcata aacctggagg cggcaaccgg atttgaaccg
      601 gtgataaagg ttttgcagac ctctgcctta ccacttggct atgccgccaa taactgggct
      661 agctggattc gaaccaacga gtgacggagt caaagtccgt tgccttaccg cttggctata
      721 gcccattaat aataagggcg gctgaagggg atcgaaccct cgaatgtcgg aaccacaatc
      781 cgatgtgtta accacttcac cacagccgcc atggcagggg cagtaggaat cgaacccaca
      841 ccaaaggttt tggagacctc tattctaccg ttgaactatg cccctattaa aaataataaa
      901 tggagggggg cagattcgaa ctgccgaacc cgaaggagcg gatttacagt ccgccgcgtt
      961 tagccacttc gctacccctc cataaatggt gccggccaga ggacttgaac ccccaaccta
     1021 ctgattacaa gtcagttgct ctaccaattg agctaggccg gctaagaaat ggttcaggac
     1081 agagtcgaac tgccgacaca tggagcttca atccattgct ctaccaactg agctactgaa
     1141 ccataataaa aatgtaatga tggcggtctc gacgggaatc gaacccgcga tctcctgcgt
     1201 gacaggcagg cgtgttaacc gctacactac gagacctata aaatattgcg ggaggcggat
     1261 ttgaaccacc gaccttcggg ttatgagccc gacgagctac cgaactgctc catcccgcga
     1321 taataaaaaa taatggcgga ggaagaggga ttcgaacccc cgcggcccgt taaggccctg
     1381 tcggttttca agaccgatcc cttcagccgg acttgggtat tcctccatta ttataggtaa
     1441 atcgctatta attataaaat taaatggcgg tctcgacggg aatcgaaccc gcgatctcct
     1501 gcgtgacagg caggcgtgtt aaccgctaca ctacgagacc attagtaaaa cggaggaaga
     1561 gggattcgaa cccccgcgag ccgttaagcc cctgtcggtt ttcaagaccg atcccttcag
     1621 ccggacttgg gtattcctcc aaaattatat ggaccttgca ggactcgaac ctgcgaccga
     1681 acggttatga gccgttagct ctaaccaact gagctaaagg tcctaaatat aattttacaa
     1741 ctaataaata gtggcggtgg aggggatcga acccccgacc tcacgggtat gaaccgtacg
     1801 ctctagccag ctgagctaca ccgccttata tagtttgtaa ataatatggt ggagactagc
     1861 gggatcgaac cgctgacctc ctgcgtgcaa agcaggcgct ctcccagctg agctaagccc
     1921 ccataataat tacagtatat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
     1981 accaagtgct ctaccaagct gagctacttc ccgtataatt aacgcgcccg ataggagtcg
     2041 aacccataac ctcttgatcc gtagtcaaac gctctatcca attgagctac gggcgcatat
     2101 atttttattg aaaatggtgc cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc
     2161 aggattttaa gtcctgtgcg tctgccagtt ccgccacccc ggcactataa aaatggagca
     2221 gaagacggga ttcgaacccg cgaccccaac cttggcaagg ttgtattcta ccgctgaact
     2281 acttctgcat atgcgggtga agggagtcga acccccacgc cgtaaggcgc tagatcctaa
     2341 gtctagtgcg tctgccaatt ccgccacacc cgcaaatggt gagccataga ggattcgaac
     2401 ctctgaccct ctgattaaaa gtcagatgct ctaccaactg agctaatggc tcttccatgg
     2461 tgccggccag aggacttgaa cccccaacct actgattaca agtcagttgc tctaccaatt
     2521 gagctaggcc ggcaatatgt aagaataaat ggtggagaat gacgggttcg aaccgccgac
     2581 cctctgcttg taaggcagat gctctcccag ctgagctaat tctccgatt
//
LOCUS       DAPIDM010000205         2616 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000205 DAPIDM010000000
VERSION     DAPIDM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.210"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..464
                     /locus_tag="RJE42_002615"
     CDS             <1..464
                     /locus_tag="RJE42_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDV5877976.1"
                     /translation="AQKYKKIYVRFLVIINVLTTITVVFALCNLYYFAAVQNHYDLFN
                     YWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHFGLLLTALSSFIYIEKI
                     IEYILIPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYIFELNTEDDD"
     gene            594..691
                     /locus_tag="RJE42_002616"
                     /pseudo
     CDS             594..691
                     /locus_tag="RJE42_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668829.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
     gene            827..1045
                     /locus_tag="RJE42_002617"
     CDS             827..1045
                     /locus_tag="RJE42_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877977.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLKIAIIVTIIVFVLNLLAYLGLR"
     gene            1153..1640
                     /locus_tag="RJE42_002618"
                     /pseudo
     CDS             1153..1640
                     /locus_tag="RJE42_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
     gene            2263..>2616
                     /locus_tag="RJE42_002619"
     CDS             2263..>2616
                     /locus_tag="RJE42_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDV5877978.1"
                     /translation="MKRILVVFLMLAIVLAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDEN"
BASE COUNT         1020 a          247 c          441 g          908 t
ORIGIN      
        1 gtgcacagaa atataaaaaa atatatgttc gatttttagt tattataaat gtgttaacta
       61 ctatcactgt agtatttgca ctgtgtaacc tttattattt tgcagctgta caaaaccatt
      121 atgatttatt caattattgg ttaatgggta cgatttcaat cataattagc tatttattat
      181 tagtaattgg cggaatgttt acgttgttaa aattacctaa agtaacaaaa cgttggggtg
      241 gtaaaactaa aacacatttc ggtttattat taactgcgtt gagctcattt atatatattg
      301 aaaaaattat cgaatatata ttgattccta atgtcgtaga atccaagttt ataattattg
      361 taagcatgtt ggttatcgct ggagcacaat ttgtggcatt tcaatttatt atgcaataca
      421 gtagattcta tatttttgaa ttaaacactg aagatgatga ctaaatgatt ttgtaaatta
      481 ctattggagt atacttatat aaaaaatcaa cttcaacgta tatcaccata ttattttata
      541 ctcaatgatg tgaaagaatt tgagtggatt gatattaatg ggatttgatt tttagaatta
      601 gaaaaggaac gcttgctttt gttgtaataa ggccaatagt tttcagttgt cggttattat
      661 tctgaatatg attttatcaa tattattata attgtattac atatagttgt cggtaaattt
      721 ttatttacga aatggtaaaa aagtatatac tattaatatc ttttttacta taattataaa
      781 tagttaaata aattgaaaga caaaataatg agtaggagtt aatgagatgg aaaaccaaaa
      841 acaaggcaat ggcttaaaaa ttgcaacatg ggtatttatt gtattaacag tagttacacc
      901 gctatttggt attggaagta ttgtttgtag tattaattac aaaaaatacg atgctgaaaa
      961 aggttcgaag ttattgaaaa ttgcaattat cgtaacaata attgtttttg ttttaaattt
     1021 attagcatat ttaggtttaa gataagtaaa atgaatttga aaaagcatta agcgacattt
     1081 gtttgttgtt tagtgctttt tatttaacgg aaatgaaact aatatctatt ttgaaaatat
     1141 aggggcgata acatgacttt cgaagagaaa ttaagtgaaa tgtataacga gattgcgaat
     1201 gagatcagtg ggatgatacc agtagaatgg gagcaagtat ttacaatagc ctatgtaact
     1261 gatcaagctg gagaagtcat ttttaattat actaaactgg tagtgatgaa ttaaattatt
     1321 attcagatat acctaaagat tgcaatgtct caaaagatat ttttaagaat tcatggttta
     1381 aagtttatcg aatgtttgat gagttaagag aaacttttaa aaaagagggt cttgaaccat
     1441 ggacatcatg cgaattcgat tttacaagag agggtaaatt aaacgtatct tttgattata
     1501 tagattggat taaattaggt tttggtccat caggaaagga aaactactat atgtataaaa
     1561 agtttggtgt tttaccagaa acggaatatg aaattaataa agttaaagaa gtagagaagt
     1621 atgttaaaga gcaagagtag cagacatgtt ataaaaggct gtgtaaaacc aacctcgtta
     1681 tacatttgac tcaaagaaga aggtaaaaga taagattatt tgcaacttaa aaagtcaatt
     1741 agtttatcgg tatccataca tcatggataa atgagttcaa ctaattaaca aattacgata
     1801 taaatttaaa attgtcatta attgaaaagg ttattgtagt gtattttata aatttgatgt
     1861 acatcgactt attttttaaa acttatatca aaaaaatatg agaaaagtat atactattaa
     1921 aatgtttttt actataatta taaatagtta aatatttggt tatgaaataa atgtgtggta
     1981 ggcaattttt agagatacat agcaatgtaa aggtatcaaa tagttattgc atcatttaat
     2041 atgatggcat aatcttcagt aaatagttgt aatttaacat tgaaatagat gtttatatta
     2101 aacagacttt aatatcataa ttaatcaaaa tcttgcgtca aacatttaaa cgaattttaa
     2161 gtttataaca attggttaaa cattgttcaa acattacaat gataaagcat attatcagta
     2221 ttgtagtgtg tggaaaatga cagccatctg aggagaaaaa tgatgaaaag aatattggta
     2281 gtatttttaa tgttagcaat tgtattggca ggttgttcta ataaaggtga aaagtatcaa
     2341 aaagatattg ataaagtgta caaagaacag aatcaaatga ataaaattgc ctcgaaagta
     2401 caaaacacta ttaaaacaga cattaaacaa gaagacagta atacacatgt ttataaagat
     2461 ggtaaagtca ttgttattgg tattcaattg tataaagatc gtgaaaaaat gtattatttc
     2521 gcatatgaaa taaaagatgg taaggcagaa atcaatagag aaatagatcc aattaagtat
     2581 atgaaagacc ataaagcaga ttatgaagat gaaaat
//
LOCUS       DAPIDM010000206         2600 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000206 DAPIDM010000000
VERSION     DAPIDM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.212"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..447
                     /locus_tag="RJE42_002620"
     CDS             <1..447
                     /locus_tag="RJE42_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV5877979.1"
                     /translation="NPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLA
                     LEEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQ
                     ADRLGAKFTIVIGDQELENNKIDVKNMTTGESETIELDALVEYFKK"
     gene            463..2229
                     /gene="aspS"
                     /locus_tag="RJE42_002621"
     CDS             463..2229
                     /gene="aspS"
                     /locus_tag="RJE42_002621"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV5877980.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINDAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            2307..2497
                     /gene="ssrS"
                     /locus_tag="RJE42_002622"
     ncRNA           2307..2497
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RJE42_002622"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          895 a          377 c          536 g          792 t
ORIGIN      
        1 aaccctaact atgatggtgc cattacaact ctttgtggtg gtggccgtta taatggttta
       61 ttagaattgc tagatggtcc aagtgaaaca ggtattggtt ttgcgctaag tatagagcga
      121 ttattgcttg cacttgaaga agaaggtatc gaattagata ttgaagaaaa cttagattta
      181 ttcattgtta caatgggtga tcaagcagat cgatatgctg tgaagttatt aaatcatttg
      241 agacataatg gtattaaagc agataaagac tacttacagc gtaaaattaa aggacaaatg
      301 aaacaagcag accgtttagg tgccaagttt acaatcgtta ttggtgatca agaattagaa
      361 aataataaaa tcgatgttaa aaatatgaca actggtgaat ctgaaacaat agaattagac
      421 gcattagtcg aatattttaa gaagtagaga gggcgttaaa atatgagtaa aagaacaact
      481 tattgtggat tagttactga ggcattttta ggacaagaaa ttacattaaa aggatgggtt
      541 aacaatcgtc gtgacctagg tggattgatt ttcgttgatt taagagatag agaaggaatt
      601 gtacaagtcg tgtttaatcc tgcattttca gaagaggcat tgaaaattgc tgaaacagta
      661 cgttctgaat atgttgtaga agttcaaggt acagttacga agcgtgaccc tgaaacagtt
      721 aaccctaaaa ttaaaactgg ccaagttgaa gtacaagtta caaatattaa agtgattaat
      781 aaatctgaga caccaccatt ttctataaat gaagaaaatg ttaacgttga tgaaaatatt
      841 cgattaaaat accgttattt agatttacgt cgtcaagagt tagcgcaaac atttaaaatg
      901 agacatcaaa ttactcgttc tattcgtcaa tatttggatg atgaagggtt ctttgacatc
      961 gaaacaccag tactaacgaa gtcaacacct gagggtgcac gtgactattt agtaccatct
     1021 cgtgttcatg atggtgaatt ttatgcatta ccacaatcgc cacaattatt taagcaatta
     1081 ttgatgatta gcggatttga caaatactac caaatcgtaa aatgcttccg tgacgaagat
     1141 ttacgtgcag atcgtcaacc tgaattcaca caagtcgata ttgaaatgag ttttgtagat
     1201 caagaagatg tgatgcaaat gggtgaagaa atgcttaaaa aagtggttaa agaagttaaa
     1261 ggcgttgaaa ttaatgacgc gttcccacgc atgacatata aagaagcgat gcgtcgctat
     1321 ggttctgata aaccagatac gcgttttgaa atggaattaa ttgacgtttc tcaattagga
     1381 cgtgatatgg actttaaagt atttaaagat actgttgaaa atgatggtga aattaaagca
     1441 attgtcgcta aaggtgcagc tgaacaatat acacgtaaag atatggatgc tttaacagaa
     1501 tttgtaaaca tctatggtgc taaaggatta gcgtgggtta aagttgtgga agatggtttg
     1561 acaggtccaa ttggacgttt ctttgaaaca gaaaatgttg aaacattact tacattaact
     1621 ggtgctgaag ctggtgactt agtaatgttt gttgcagaca aaccaaatgt cgttgcacaa
     1681 agtttaggtg cattacgtgt caaattagct aaagaattag gtttaatcga tgaaacaaaa
     1741 ttaaacttct tatgggtgac agattggcca ttattagaat atgatgaaga tgcgaaacgt
     1801 tacgttgcag cacatcatcc gtttacatct ccaaaagaag ctgatattgc taagcttggc
     1861 actgcgccag aagaagctga ggcaaatgct tatgacatag tattaaatgg ttatgaatta
     1921 ggtggcggtt caatcagaat tcatgacggt gagttacaag aaaaaatgtt cgaagttctt
     1981 ggatttacta aagaacaagc acaggaacaa ttcggcttct tactagatgc atttaaatat
     2041 ggtgcaccac ctcatggcgg tattgcatta ggattagacc gattagtcat gttattgact
     2101 aatagaacaa acttgagaga tacaatcgca ttccctaaaa cagcatctgc tacatgttta
     2161 ttaacgaatg cgcctggtga agtttctgat aaacaattag aagaactttc cttgcgaatt
     2221 cgtcactaaa ttttaaaaaa tcaataaact ttggtttgtt ataatttcat taattttaat
     2281 tatgtggtaa tataacatca taagatagtc atctgagttg ttcgtaagtt tgctttttaa
     2341 tttgggccta acactctttg atcaagggag cccaatgggt tttcttgcag cgtacacgcc
     2401 ttatatgaag gacgtacaaa acaagaaaca gggcacccac ctgtatataa caggccgaat
     2461 gatcaagcta ttataactac ggcaatacgg actctatcga tacgcaagac tttgtcttgc
     2521 gtatttttat gcttttcttt aagtttttaa taaagaatta gttgggttta atagcgttta
     2581 ataattgttt tctcgttgaa
//
LOCUS       DAPIDM010000207         2589 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000207 DAPIDM010000000
VERSION     DAPIDM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.213"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..169
                     /locus_tag="RJE42_002623"
     CDS             <1..169
                     /locus_tag="RJE42_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1806 family protein"
                     /protein_id="HDV5877981.1"
                     /translation="EPYRIGLKLSNGGWVYVQGLTHFEINEHDEFLIAGFNYEGQLAA
                     ALQISERPFNL"
     gene            183..848
                     /gene="bshB2"
                     /locus_tag="RJE42_002624"
     CDS             183..848
                     /gene="bshB2"
                     /locus_tag="RJE42_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDV5877982.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQQGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPTIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            861..1739
                     /locus_tag="RJE42_002625"
     CDS             861..1739
                     /locus_tag="RJE42_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDV5877983.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            2021..>2589
                     /locus_tag="RJE42_002626"
     CDS             2021..>2589
                     /locus_tag="RJE42_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HDV5877984.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIV"
BASE COUNT          921 a          401 c          457 g          810 t
ORIGIN      
        1 agaaccttat cgcattggtc ttaaactatc aaatggtggt tgggtttatg tacaaggtct
       61 aacgcatttt gagatcaatg agcatgatga atttttaatt gcaggtttta attacgaagg
      121 tcaactcgct gcagcacttc aaattagtga gcgaccattt aatttataga ggaggcatca
      181 acatgactga cgaaagacat gtattagtaa tatttccaca tcctgatgat gaaaccttct
      241 catctgcagg cacattagca agttatatcc aacaaggcat acctgtaaca tatgcatgtt
      301 taaccctagg acaaatgggc agaaatttag gtaatccccc atttgcaaca cgcgaatcat
      361 taccaactat tcgtgagcgt gaactagaag aagcttgtaa agttattggt attactgatt
      421 taagaaaaat gggtttacgt gacaaaactg tcgaatttga accctatgaa catatagatg
      481 gtatgattaa atctttaatc gatgatacaa atccatcatt gattatttca ttttaccctg
      541 gatatgcagt acaccctgat cacgaagcaa cagcagacgc tgtaattcga acggttgaac
      601 gcatgcctaa agaagaacgc cctcgtttaa ctttagttgc attcagtaat gatgcaaccg
      661 aagcacttgg agaacctgat atccaaaatg atattacaga ttttaaagaa cttaaaatta
      721 aggcatttga agcacatgct tctcaaacag gtccattttt aaaacaatta gcaagtcctg
      781 aaatcgatgg taaacaacac agctttttaa cagtcgaacc atattggaca tatcacttta
      841 aatcttaacg gaggaaaaaa atgactgaat ttgatttatc gacacgagaa ggtcgttgga
      901 aacactttgg ttccgtagat cctattgaag gcacgaaacc tactactaag aacgaaatga
      961 cagatttaca aagtacacat aaagatttct tatttgaaat agaagaagtt ggtattaaaa
     1021 acttagtgta ccctgtactt gttgaccagt atcaaacagc tggcacattt agtttttcaa
     1081 caagtttaac aaaagacgaa aaaggtatta atatgagccg tattattgaa agtgttgaaa
     1141 agcactatga taatggtatt gaacttgaat ttaatacact ttatcaggtg ttacgcactt
     1201 tacaaacgaa tatgaaacaa aatgcggcag gcgttgatgt ttctgggaaa tggttctttg
     1261 atcgctatag tccaaccaca aatattaaag cagttggtaa tgccgatgtc acttatggat
     1321 tagcaattga cggtgataaa gttacacgta aagaacttac tattgaagca actgtcacaa
     1381 cgctatgccc ttgttcaaaa gaaataagtg aatattcagc gcacaaccaa cgtggtgttg
     1441 tcactgttaa aacatatatt aataaagatc aagacatagt tgatgactat aaaaacaaaa
     1501 ttttagatgc aatggaagct aatgcaagtt caattctata ccctatttta aaacgtcctg
     1561 atgaaaaacg tgtgacagag cgtgcatatg aaaatccacg ttttgtcgaa gacttaattc
     1621 gtttaattgc cgcggactta gttgaatttg attggttaga cggtttcgat atcgaatgtc
     1681 gtaatgaaga gtctatccat caacatgatg ctttcgctaa attaaagtat cgcaaataat
     1741 atcatatgat taattcaaac gcgtaacagt agttgcgcgt ttttactata cctcctagag
     1801 ttgtataact cttttataaa tctattcatt tgatgtgcct gagtcataaa tcaaattcct
     1861 aagctctaca atgatatatt ggtcgtagtt gactgaataa ctactaccat tataaatatc
     1921 aaaacttgtt gttttataaa taaacacatc ttccagttaa aagaagtaat taaatattat
     1981 gtgatataat tataaaaaat agatataagt ggtgttgtaa atgaattatt ttgtaggtaa
     2041 tagtttaggt gttaatttaa ctgggataga gaaagcaatt attaatcgtt taaatttatt
     2101 taaagaaatg ggaagaccag cacaatgtgt atttctgtca tggaatagat atttatatag
     2161 aaatgcccaa aattatatca ctagttctga ctatattaat atgtatgatt tcttccaaga
     2221 agcaacatat ttagaacgca atgaaccatt tgactggttg tcatattgga ctgatgaatg
     2281 tcattatact ttaaaacatg ttgaaaatag tcatgacttt agaatatatg atcaagagcg
     2341 tttcttaatg tatgcccatt ttcaagatcc taaatatcgt atattagatt atgtgaatca
     2401 ttttgatagt caaagacgaa aagtaaaacg tgatttttat gatgtgcgtg gctttttaag
     2461 ttgtagtagg attcttgttg ataaacaaca aacattatgt gaatttttct ataatccaga
     2521 aggcgacaca aaattagaaa agtatttttc ttacaaagat ggcaaaccag aagtacaaaa
     2581 aattattgt
//
LOCUS       DAPIDM010000208         2556 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000208 DAPIDM010000000
VERSION     DAPIDM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.215"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            84..305
                     /locus_tag="RJE42_002627"
     CDS             84..305
                     /locus_tag="RJE42_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDV5877985.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            315..719
                     /locus_tag="RJE42_002628"
     CDS             315..719
                     /locus_tag="RJE42_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDV5877986.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRPITYIADFSLWKDGKLVEVLDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            724..909
                     /locus_tag="RJE42_002629"
     CDS             724..909
                     /locus_tag="RJE42_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDV5877987.1"
                     /translation="MQQQAYINATIDIRIPTEVEYKHFGDVDNEKDALADYLYNNPNE
                     ILEYDNLKIRNVNIEVE"
     gene            910..1215
                     /locus_tag="RJE42_002630"
     CDS             910..1215
                     /locus_tag="RJE42_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877988.1"
                     /translation="MARITKETKTVSDGYSREDRETTLNYDYENQEWIAYSSVPTHIT
                     RMTKLYGDDVEVLERLESGTAVLVRAKLPKSAIGFRKLMSEERRQELSERAKRAFGH"
     gene            1343..1699
                     /locus_tag="RJE42_002631"
     CDS             1343..1699
                     /locus_tag="RJE42_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877989.1"
                     /translation="MSIVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LHAPYGMRLAEYKEIVLSKIMERESKEREMARQRRKEAELRRKKPHLFNVPQKHPRGR
                     YACYLMENDIFVKVKK"
     gene            1703..1945
                     /locus_tag="RJE42_002632"
     CDS             1703..1945
                     /locus_tag="RJE42_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877990.1"
                     /translation="MTDNALKEYLRRFFGSKRYLYQDNERVAHIHVVNDTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQHDLEYEEQKQLTLF"
     gene            1959..2165
                     /locus_tag="RJE42_002633"
     CDS             1959..2165
                     /locus_tag="RJE42_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877991.1"
                     /translation="MKIKIEKEMNLPELIQWAWDNPKLSGNKRFYSNDVERNCFVTFH
                     VDSILCNVTGYVSINDKFTVQEEI"
     gene            2168..>2556
                     /locus_tag="RJE42_002634"
     CDS             2168..>2556
                     /locus_tag="RJE42_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877992.1"
                     /translation="MKIKVKKEMRLDELIKWAWDNPGLATGRNFYPQTKSDIDYKCFS
                     LYDGRNCIIKGFVSADDTFEVEFEEDITEETKVDRLIELFEIQEGDYNSTLYENTSIK
                     ECLYGRCVPTKAFYILNDDMTMTLIWK"
BASE COUNT         1007 a          352 c          548 g          649 t
ORIGIN      
        1 cctttcgatg attacgacag agttgaagag aaaattatga atgaaatcaa ttggaagaaa
       61 acgcacatta aggagtgtta aaaatgccga aagaaaaata ttacttatac cgagaagatg
      121 gcacggaaga tattaaggtc atcaagtata aagacaacgt aaatgaagtt tattcgctca
      181 caggagccca tttcagcgac gaaaagaaaa ttatgactga tagtgaccta aaacgattta
      241 aaggcgctca cggacttcta tatgagcaag agctaggttt acaagcaacg atatttgata
      301 tttagaggtg gacgatgagt aaatacaacg ctaagaaagt tgagtacaaa ggaattgtat
      361 ttgatagcaa agtagagtgt gaatattacc aatatttaga aagtaatatg aatggcacta
      421 actatgatcg tatcgaaata caaccgaaat tcgaactaca acctaaattt gggaaacaaa
      481 gaccgattac gtatatagct gatttctctt tgtggaagga tggcaaactg gtcgaagttt
      541 tagatgttaa aggtaaggcg actgaagttg ccaacatcaa agcgaagata ttcagatatc
      601 agtatagaga tgtgaattta acgtggatat gtaaagcacc taagtacaca ggcaaaacat
      661 ggattactta cgaggaatta attaaagcaa gacgagaacg caaaagagaa atgaagtgat
      721 ctaatgcaac aacaagcata tataaacgca acgattgata taagaatacc tacagaagtt
      781 gaatataagc attttggtga tgtggataac gaaaaagatg cgctggcaga ttacttatat
      841 aacaatccta acgaaatact agagtatgac aatttaaaaa ttagaaacgt aaatatagag
      901 gtggaataaa tggcaagaat taccaaagaa acaaaaactg taagcgacgg ttattcaaga
      961 gaagaccgag aaacgacatt gaactatgat tacgaaaatc aagaatggat tgcttactca
     1021 tcggtaccga cacatattac tagaatgaca aagttgtacg gcgatgatgt agaggtattg
     1081 gaacgattag aatctgggac tgcggtattg gttagggcga aactacctaa aagcgcaata
     1141 ggttttagaa aattaatgtc tgaagagcga cgacaagaat tatctgagag agcaaaaaga
     1201 gcttttggtc attagtgctc gtgaatatag ggcgaaaaac gaccaaaaag acacactaat
     1261 actttttagg ataaataaca tccggagaaa aaaacatgag ctttaaaaat tttaacacag
     1321 gataaataca gaggtggaat aaatgagtat cgtaaagatt aacggtaaac catataaatt
     1381 taccgaacat gaaaatgaat tgataaaaaa gaatggttta actccaggaa tggttgcaaa
     1441 aagagtacga ggtggctggg cgttgttaga agccttacat gcaccttatg gtatgcgctt
     1501 agctgagtat aaagaaattg tgttatccaa aatcatggag cgagagagca aagaacgtga
     1561 aatggctagg caacgacgta aagaggctga gctaagaaga aagaagccac atttgtttaa
     1621 tgtaccacag aaacatccaa gaggacgtta tgcgtgctac ctgatggaaa acgacatatt
     1681 cgtgaaagtt aagaagtaga tcatgacaga taacgcactc aaagaatact taagacgatt
     1741 tttcggctct aagagatatc tgtatcagga taacgagcga gtggcacata tccatgtagt
     1801 aaatgacact tattactttc atgggcatat cgtaccaggt tggcaaggcg tgaaaaagac
     1861 atttgataca gccgaagagc ttgaaacata tataaagcaa catgatttgg aatatgagga
     1921 acagaagcaa ctaactttat tttaaaaggg cggaaacaat gaaaatcaaa attgaaaaag
     1981 aaatgaattt acctgaactt atccaatggg cttgggataa ccccaagtta tcaggtaata
     2041 aaagattcta ttcaaatgat gttgagcgca actgttttgt gacttttcat gttgatagca
     2101 tcttatgtaa tgtgactgga tatgtatcaa ttaacgataa atttactgtt caagaggaga
     2161 tataacaatg aaaatcaaag ttaaaaaaga aatgagatta gatgaattaa ttaaatgggc
     2221 atgggataac cctggattag caacaggaag aaatttttat ccacaaacca agagtgatat
     2281 tgattataag tgcttctctc tttatgacgg aagaaattgt atcataaaag gttttgtatc
     2341 agctgatgat acttttgaag tcgaatttga agaagatatt acagaagaga ctaaggttga
     2401 taggttgatt gaattattcg agattcaaga aggagactat aactctacac tatatgagaa
     2461 cactagtata aaagaatgtt tatatggcag atgtgtgcct actaaagcat tctatatctt
     2521 aaacgatgac atgacgatga cattgatttg gaaaga
//
LOCUS       DAPIDM010000209         2403 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000209 DAPIDM010000000
VERSION     DAPIDM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.216"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            105..1280
                     /locus_tag="RJE42_002635"
     CDS             105..1280
                     /locus_tag="RJE42_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDV5877993.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1258..>2403
                     /locus_tag="RJE42_002636"
     CDS             1258..>2403
                     /locus_tag="RJE42_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV5877994.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAV"
BASE COUNT          790 a          377 c          407 g          829 t
ORIGIN      
        1 aaaatgctat aaatgtaacg actttgcgtc tttatttctt ttataatgcc gtaaaataag
       61 ttacacttaa ataaaatttt caaactaacg gagggtggct attaatggtt actcaaaata
      121 aaaagatatt gattattact ggctctttcg gtaacggtca tatgcaagtt acacagagta
      181 tcgttaatca acttaatgat atgaatctag accatttaag cgtcattgag cacgatttat
      241 ttatggaagc tcacccaatt ttgacttcta tttgtaaaaa atggtatatc aatagcttta
      301 aatattttag aaatatgtac aaagggtttt attacagccg cccagataaa ctagacaaat
      361 gtttctacaa atactatgga cttaataaat taattaattt attgataaaa gaaaagccgg
      421 atttaatatt attaacgttt cctacaccag ttatgtcggt actaactgag caatttaaca
      481 ttaatattcc agttgctaca gtgatgacag actatcgctt acataaaaac tggattacgc
      541 cgtattcaac aagatattat gtggcaacaa aagaaacgaa acaagacttc atagacgtag
      601 gtattgatcc ttcaacagtt aaagtgacag gtattcctat tgataacaaa tttgaaacac
      661 ctattaatca aaagcagtgg ttaatagaca acaacttaga tccagataag caaactattt
      721 taatgtcagc tggtgcattt ggtgtatcta aaggttttga cacgatgatc actgatatat
      781 tagcgaaaag tgcaaatgca caagtagtta tgatttgtgg taagagcaaa gagctaaagc
      841 gttctttaac agctaagttt aaatctaacg agaatgtatt gattctaggt tataccaaac
      901 acatgaatga atggatggca tcaagtcaac ttatgattac gaaacctggt ggtatcacaa
      961 taactgaagg tttcgcccgt tgtattccaa tgattttcct aaatcctgca cctggtcaag
     1021 agcttgaaaa tgccctttac tttgaagaaa aaggctttgg taaaatcgct gatactccag
     1081 aagaagcaat aaaaattgta gcaagtttaa cgaacggtaa tgagcaatta actaacatga
     1141 ttagtacaat ggaacaagat aaaattaagt atgctacaca gacaatatgt agagacttac
     1201 ttgatttaat cggtcattca tcacaaccac aagagattta tggaaaggtt cctttatatg
     1261 caagattctt cgttaaataa ttacgctaat cataaaaatt tcattttaat gcttattatc
     1321 ttatttttaa tggaatttgc gagaggcatg tatatcctaa gctatattaa tttcttgcca
     1381 acagtaacat ccattgctgt agcaataacg tctctagcat tttcaataca ttttattgct
     1441 gatgcatcta caaactttgt tataggcttt ttattgaaaa aattcggcac aaaaatcgtt
     1501 ttaacgactg gatttatttt agcatttaca agtttatttt tagttatttg gtttccagca
     1561 tcaccttttg taatcatatt tagtgctatg atgctcggta ttgcagttag tcctatttgg
     1621 gtcattatgt tatccagtgt tgaagaagat aaacgtggca aacaaatggg ctatgtttat
     1681 ttttcatggt tgttaggcct attggtaggt atggttttta tgaatctact tatcaaagtg
     1741 cacccaacac gctttgcctt catgatgtct ttagtcgttt taattgcttg gatattatat
     1801 tattttgtcg atgtaaagtt aacaaattat aatactcgtc cagttaaagc tcaattgaga
     1861 caaattgttg atgtgacaaa gcgccattta ttgttgttcc ctggtattct tttacaaggt
     1921 gccgcaattg ctgccctagt tcctatatta ccaacatatg ctactaaggt tattaacgta
     1981 agcacaattg aatatactgt tgcaatcatt attggtggta tcggctgtgc agtttcgatg
     2041 ctatttttat cgaaactgat agataaccgt agcagaaact ttatgtatgg tgtcattcta
     2101 agcggattta ttttatacat gatattaatt tttactctat ctatgattgt taatattcac
     2161 atcgtgtgga ttatcgcttt agctatcggt ctaatgtatg gcatcttatt accagcatgg
     2221 aataccttta tggcacgatt tatcaaatca gatgaacaag aagaaacttg gggtgttttt
     2281 aatagtatcc aaggattcgg ctcaatgatt gggccattat ttggcggatt aattacacaa
     2341 tttaccaaca atttaaacaa tactttttat ttctcggcat taatcttttt agtcttagct
     2401 gta
//
LOCUS       DAPIDM010000210         2352 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000210 DAPIDM010000000
VERSION     DAPIDM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.217"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..342
                     /gene="rnpB"
                     /locus_tag="RJE42_002637"
     ncRNA           <1..342
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RJE42_002637"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            551..1696
                     /locus_tag="RJE42_002638"
     CDS             551..1696
                     /locus_tag="RJE42_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDV5877995.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIKVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVNRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            1780..2112
                     /locus_tag="RJE42_002639"
     CDS             1780..2112
                     /locus_tag="RJE42_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDV5877996.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT          854 a          324 c          466 g          708 t
ORIGIN      
        1 atgctcacac agtctgagat gattgtagtg ttcgtgcttg atgaaacaat aaatcaaggc
       61 attaatttga cggcaatgaa atatcctaag tctttcgata tggatagagt aatttgaaag
      121 tgccacagtg acgtagcttt tatagaaata taaaaggtgg aacgcggtaa acccctcgag
      181 tgagcaatcc aaatttggta ggagcacttg tttaacggaa ttcaacgtat aaacgagaca
      241 cacttcgcga aatgaagtgg tgtagacaga tggttatcac ctgagtacca gtgtgactag
      301 tgcacgtgat gagtacgatg gaacagaaca tggcttatag aaatatcact actagtttag
      361 ctctcctaga tgatggagag cttttttcat gaaaagaaca cttaaaatta acgccttgtc
      421 ttgatataat gacactgcct tgttttaaaa taataagcga atgcgttaac gtatcagcga
      481 ttaaatttgg tggaaatgta taaaaaacac aagctaagaa taaaatacct gtataaaagg
      541 agaatcatat atgtttcaat tacttgcagt ttgtccgatg ggattagaag ctgttgttgc
      601 tagagaaatt caagaattag gctatgaaac aaatgttgaa aatggtcgta tattttttga
      661 aggagacgca agtgcaattg taaaagcaaa tttatggttg cgcacagcag accgaatcaa
      721 aattgttgtt ggacgtttta acgcaacaac gtttgacgaa ttattcgaac aaaccaaagc
      781 gctcccttgg gaatctatta ttgataaaga aggtaacttc ccagttcaag gtagaagcgt
      841 taaatcaaca ctacatagtg tgccagattg tcaggcgata actaagaaag ctattgttga
      901 acgattacgt cgcgcatata acgaaaaagg ttggttaaat gaatcaggtg ccaaataccc
      961 tgttgaagtt gccattttaa aagataatgt attattgact atcgacacat caggttctgg
     1021 tttgaacaga cgtggttata gattagcaca aggtgaagca ccaattaaag aaacgttggc
     1081 agcaagttta atccgtcttg caaactggaa aggtgataca cctttaattg atccattttg
     1141 cggttcgggt acaatagcta tagaagcttg tttaattgct caaaatatcg cacctggttt
     1201 taatcgcgag ttcgtatcag agcaatggaa catcatgcca gcaaatattt atgatgatta
     1261 ccgtgatgaa gccgataaga tggctgatta tgataaagaa atcaaagtat atgcttctga
     1321 tatcgatcca gaaatggtag agattgctaa gcgtaacgct gaagaagttg ggttgtctga
     1381 tattattaaa tttagtgtaa aagatgtcaa tacattaaca attgatacag aagaaccagt
     1441 ggcgttaatt ggaaatcctc catatggtga acgtattggt gatcgtgaag aagttgaaga
     1501 aatgtaccgt tatattggta aactaatgaa acaacatcca tttttatcta catacatttt
     1561 aacaagtaat aaagaatttg aatatttagt aaatcgtaaa gcaacgaagc gacgtaagtt
     1621 atttaatgga tatatagaat gtacttatta tcaatactgg ggtaaaaaga cagaacgtaa
     1681 aactattgaa aactaaagtg tttctaatgc gtgactaaaa ttagtaataa ttaagttctc
     1741 atgataatag gtatttttga aaaatggagg agtctataaa tgggtaaaaa aatgggtcta
     1801 ggtttatcta tagcattggt tgttattggt attgccgttg tatgtttaat gattttttct
     1861 agtcaaaaaa cgacttattt tggttatatg aatagtaata caaatgcaga aaaagttgtc
     1921 agtgaaaaag atggattagt caaacataat atcaaagtag aaccatcaaa tgattttaaa
     1981 ccgaaaaaag gagactttgt aaaattagtt tctaaagatg atgggaagac attttataaa
     2041 caagagattg ttaaacatga tgacgtccca cacggtttaa tgatgaaaat tcacgacatg
     2101 catatgaatt aataaaaagc atctataacg taatttggaa gaagtaaagt aatcttctta
     2161 tacgttttag atgctttttt tatcttcaaa ttcaaaaaat cggacaaatg aaaagtaaaa
     2221 aacttatcca tcagtacgat ttattataga acccgtttta agtaattaga gaatcgagaa
     2281 tcatttttta aatttcgtca gttcaaaact ttaagtgcat tatagtttag cacgacgatc
     2341 tttaatatat aa
//
LOCUS       DAPIDM010000211         2352 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000211 DAPIDM010000000
VERSION     DAPIDM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.218"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..491)
                     /locus_tag="RJE42_002640"
     CDS             complement(<1..491)
                     /locus_tag="RJE42_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5877997.1"
                     /translation="MSIPINLPTNSTMINELCTLQSRTINIKGEVLITEIYDDYFFKN
                     DEWHITAFNKFKQFQDSIKNYRDKRKNVFFRIKSKNLNLEFKYLFLKLIVKEDWSLSN
                     LFNTGAVKLNKIAKFFNEVYPNLNSLLDCDINTLEKHWFNWLTENNIPIKRRSSTIVF
                     GDY"
     gene            complement(494..1606)
                     /locus_tag="RJE42_002641"
     CDS             complement(494..1606)
                     /locus_tag="RJE42_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV5877998.1"
                     /translation="MKIVEVKSKNGTNFMILDGNNEPIVDAVRYLKYLDSVKKSLNTK
                     KTYAYALKNFFVYLESKKICYKEVSFDNFVDFIRWMKTPFEYENVLSYHRKEKSISPK
                     TINLTMTVVSNFYDYLYRSKKLDVNFYDFMHMESKYSKKYKSFMHHINKDYRTLKNIL
                     KVKEPKKKIEVLTNAEVKKLLEEANNIRDKFLIQLLYETGLRIGEVLSLRIDDIKFDF
                     RKGHQIVLKNRFNDNGTYLKTGERKIFISQSLIDLYDDYVYEIIDELSICSDYLFVKI
                     KGRNVGEAMNYSDIYSLFKRLKHKTSINVHPHLFRHTHATVFYNETKDIKQVQERLGH
                     SNIQTTINLYVHPTEEDIREDWNKVKHQFQVFNKGE"
     gene            1791..2096
                     /locus_tag="RJE42_002642"
                     /pseudo
     CDS             1791..2096
                     /locus_tag="RJE42_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
     gene            complement(2153..2326)
                     /locus_tag="RJE42_002643"
     CDS             complement(2153..2326)
                     /locus_tag="RJE42_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5877999.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT          810 a          374 c          238 g          930 t
ORIGIN      
        1 tcataatcac caaaaactat tgttgatgat cttcttttta ttggtatatt attttcagtc
       61 agccaattaa accagtgctt ttctaatgta tttatatcac aatcaagtaa agaatttaaa
      121 tttggataaa cttcattaaa gaacttagca atcttattaa gctttactgc tccagtatta
      181 aataaattag ataaggacca atcctcttta actatcagtt tcaaaaaaag gtatttaaat
      241 tctaaattca aatttttact tttaatccta aagaaaacat tttttctttt gtctctatag
      301 ttcttgatac tatcttgaaa ttgcttgaat ttgttaaaag ctgtaatgtg ccactcatca
      361 tttttaaaaa agtaatcatc atatatttca gttattaaaa cctctccttt tatattgatg
      421 gttctacttt gtaatgtaca taattcatta atcatagtac tgtttgtagg taaattaatt
      481 ggtattgaca tagtcattcc cctttattaa aaacttgaaa ttgatgcttt actttattcc
      541 aatcttcacg tatatcttct tcagtgggat gaacatataa attaatagtc gtttgtatat
      601 tactatgacc aagtctttct tgaacttgtt ttatatcctt agtttcatta taaaaaacag
      661 ttgcatgtgt atggcgaaat aaatgaggat gaacatttat agatgtttta tgttttagcc
      721 ttttaaataa tgaatatata tcactataat tcatagcttc tcctacattt ctccccttta
      781 tttttacaaa aagataatca gaacatattg aaagttcgtc aattatttcg tagacataat
      841 catcgtataa atcaattaac gattgggaaa taaatatttt tctttcccca gttttcaaat
      901 aagtaccatt atcattaaat ctatttttca aaactatttg atggcctttt ctaaagtcaa
      961 atttaatatc atcaatacgt aatgataata cctcacctat acgtaatccg gtttcatata
     1021 gtaattgtat taagaattta tctctaatat tattagcttc ctctaataat ttcttaacct
     1081 ccgcattagt taacacttct atttttttct ttggttcttt aactttcaaa atatttttca
     1141 acgttctata gtccttattt atgtgatgca tgaaactttt atatttttta gagtatttac
     1201 tttccatatg cataaaatca tagaaattaa catctaattt tttactccta tagagataat
     1261 cataaaaatt agatactaca gtcatagtca gattaattgt cttaggacta atgctttttt
     1321 cttttcggtg ataagagagg acattctcat attcaaaagg tgttttcatc catcttataa
     1381 aatcaacaaa gttatcaaaa ctaacttctt tatagcatat ctttttactt tctaagtaaa
     1441 caaaaaaatt ttttagtgca taggcatagg ttttcttggt atttaaactt ttcttaacac
     1501 tatccagata cttcaaatat cttactgcat ctactatagg ttcattatta ccatctaaaa
     1561 tcataaaatt ggtaccattc ttagatttta cttctactat tttcataata atctcatttc
     1621 taattttaat agtataagta tacactattc aaattctttt tacatcatac atattaaact
     1681 tattacacgt aataaaaaaa agactattta atatttttac tttgttcatt ttttcttaaa
     1741 atatgattca ttttaaacaa aatagtctat attcttcaac atatatataa ccttttgcaa
     1801 cattacccga acaattcgaa acgattcaac aatacattat cgaccaaaac aaaattacac
     1861 gtcctgtatt atcagatgat caattggctg agctaaacat tcggctacac gaggcgctac
     1921 aatatgccca acctgtagaa gttaaatttt ataataatgg ctttgtagat tcagtacgct
     1981 taaccattta tcgtattgat gctattaatt atgaaattga tggttatgtt tacaatcagc
     2041 aacaacgtca aaaaatttct atctttgata tcttagatat tgtcttgtta ccttaatgct
     2101 ttgaacataa ttaaaggcaa gaacaatcaa tattgtcctt gcctaaatgt tattagttat
     2161 ttttactttc gatatcagaa ataatttctt tagtttttct ttcaatatct tcaaaatctt
     2221 ttttaaagat ataagcaaat gctactacac caacaccagc aactaatgtt gtaatagcaa
     2281 ctaagctaaa tacgaattta aacacacatt taataaaatt ccacatctca atttcacctc
     2341 tgttaaatta aa
//
LOCUS       DAPIDM010000212         2333 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000212 DAPIDM010000000
VERSION     DAPIDM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.219"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..744
                     /gene="queA"
                     /locus_tag="RJE42_002644"
     CDS             <1..744
                     /gene="queA"
                     /locus_tag="RJE42_002644"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV5878000.1"
                     /translation="LLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGIL
                     QERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKG
                     VNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGT
                     TSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFS
                     NRENVLNAYKTAVNLEYRFFSFGDAMLII"
     gene            767..1906
                     /gene="tgt"
                     /locus_tag="RJE42_002645"
     CDS             767..1906
                     /gene="tgt"
                     /locus_tag="RJE42_002645"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV5878001.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            1925..2185
                     /gene="yajC"
                     /locus_tag="RJE42_002646"
     CDS             1925..2185
                     /gene="yajC"
                     /locus_tag="RJE42_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV5878002.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
BASE COUNT          830 a          335 c          452 g          716 t
ORIGIN      
        1 ttactgaaac cagctaagcg tattaaagtt ggtaataaat tgaattttgg taatggcaaa
       61 attatagctg aatgcataaa agaaatggac caaggtggac gtatcatgcg tttacattat
      121 gaaggtattt tacaagaaag attagatgaa ttaggggaaa tgccactgcc accatacatc
      181 aaagaacgtt tagatgatcc agatcgttat caaacagttt acgctaaaga aagtggttca
      241 gcggcagcac caacagctgg attacatttt actgatgagt tattaactga aattaaaaat
      301 aaaggtgtta atatcgcatt tgttacatta catgttggat taggtacgtt tagaccggtg
      361 agcgtcgacg atgtgaatga ccacgaaatg catagtgaat attatcaaat gacacaagaa
      421 acagctgatt tattaaatga tactaagtcc aaaggacatc gcattatatc agttggtaca
      481 acttcaacac gtacacttga aacaattcga cgcgatcatg ataaatttgt tgaaacgagt
      541 ggctggacta atatatttat ttatccagga tttgatttta aagctattga tggccagatt
      601 actaattttc atttaccaaa atcaacatta gttatgctag tatcagcgtt tagtaatcgt
      661 gaaaatgttc tgaatgctta taaaacggca gtaaatttag aatatagatt ctttagtttt
      721 ggcgatgcaa tgttaattat ttaaaaagaa tgtgaggatt ttgaatatgc ctgcagtaac
      781 atacgaacac attaaaactt gtaaacaatc aggtgcgcgt ttaggtatcg tgcacacacc
      841 acacggttca tttgaaacac ctatgtttat gccagttggt actaaagcaa ccgttaaaac
      901 aatgagtcca gaagagttaa gacaaattga agcaaaaatc attttgggca acacatatca
      961 tttgtggtta caacccggaa atgatattat caaacacgct gggggattac ataaattcat
     1021 gaattgggat ggtccgattc taacagattc aggcggtttc caagtgttta gtttaagtaa
     1081 tttacgtaaa attacagaag aaggcgtgga atttagacat catactaatg ggtctaaatt
     1141 atttttgagt cctgagaaat caatgcaaat tcaaaatgat ttaggttctg atattatgat
     1201 ggcatttgat gaatgtccac cgatgcctgc tgaatatgat tatgtaaaaa aatctattga
     1261 acgtacaaca cgttgggcga aaagatgtct agatgcacac caaagacctg aagatcaagc
     1321 attgttcggc attatacaag gtggcgaata tgaagattta agagaacaaa gtgcaaagga
     1381 tttagtagaa ttagattttc ctggttatgc aatcggcggt ttgtcagttg gtgaacctaa
     1441 accggttatg tataaaatgg ttgaacatac agagcagttt atgcctaaag ataaaccaag
     1501 atatttaatg ggtgtaggat ctccagatgc gttaatcgaa tgtagtattc gcggcatgga
     1561 tatgtttgat tgtgtcttac cgacacgtat tgccagaaat ggtacttgta tgacatcgca
     1621 aggtcgttta gttattaaaa atgcaaaatt tgcagatgat ttaagaccgt tagatgagaa
     1681 ttgtgactgt tatacatgtc aaaactactc aagagcgtat atacgtcatt taatcaaggc
     1741 agaggaaact tttggtattc gtcttactac tattcataat ttacattttc tgctaaaatt
     1801 aatggaagat ataagacaag ccattcgaga agatcgtctt ttagatttca aagaagaatt
     1861 cttcgagcaa tatggattaa atgttgagaa cccaaaaaac ttttaagcaa gaggagcgta
     1921 taaaatgcaa ttttcattac taatatatat agtcgtaatt tttgcggtta tgtatttctt
     1981 gatgatcaga ccacaacaaa aacgtgcgaa acagcatcgt gagttgatta ataacattca
     2041 atctggtcaa agaattacaa ctattggtgg tattaaaggt actgttaaag cagtagatga
     2101 aacaactgtt gttattacag ttaatggtca tggtactgaa ttaactttcg aaaaacctgc
     2161 tattaaacaa gttgaccctt cataacaaga agtggtatta aaaaggatga aacgaatagc
     2221 gtttcatcct ttttaagtta taagtagaat ttagaatagc ttatttttat tccaaaaaaa
     2281 gaaattgtga tacataataa ttgttaaatt taaattaaat atatctatga cca
//
LOCUS       DAPIDM010000213         2332 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000213 DAPIDM010000000
VERSION     DAPIDM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.220"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..188)
                     /locus_tag="RJE42_002647"
     CDS             complement(<1..188)
                     /locus_tag="RJE42_002647"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HDV5878003.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIG"
     gene            502..1212
                     /gene="deoD"
                     /locus_tag="RJE42_002648"
     CDS             502..1212
                     /gene="deoD"
                     /locus_tag="RJE42_002648"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV5878004.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1333..1776)
                     /locus_tag="RJE42_002649"
     CDS             complement(1333..1776)
                     /locus_tag="RJE42_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDV5878005.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLEKAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(1976..>2332)
                     /locus_tag="RJE42_002650"
     CDS             complement(1976..>2332)
                     /locus_tag="RJE42_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HDV5878006.1"
                     /translation="LIQHDLPRNYQFATLKGEVGQQFFKQQPEAANKNSVILQKGDRI
                     YFESQAIIQLITALPNRTKLLGVGLWLVPKPVRNFGYRMFANNRNRMWKTTWHQPNDY
                     EKSFFLDDNAKVKLTD"
BASE COUNT          736 a          396 c          348 g          852 t
ORIGIN      
        1 aatcctatta ccgtacaaac gagcacctct gaatcagcta gtcgctctgc tgcatattta
       61 acatgcgttg gattcacaca tacagattta aaattgtatg ctttcgcttc atcgatgatt
      121 tgatcgattt gcgtacgtgt tgactcaggc ttcagcaaag tgtgatcaat caattttgca
      181 ctattcattt tctacctcct cctttatgat tgattataaa aatacggttg taaattagtt
      241 gattgagcgt caggttcatt taaatatcag gttagatgtc cgcttttatg taaccgcata
      301 catatactgt tacattaatt catttcccat aaacaaacaa tacaattgaa catgatatct
      361 tcattatgaa cgatgacttg ccaacaagct aatcaggatt atatttttat aattctttaa
      421 ttctatagta caaaaattcg caaaaaaggg aaacaaatgt tatcttaaaa ttattaatga
      481 atattaagga gaagataaca aatgacaaaa ggtacaccac atattcaacc aaatggagta
      541 aaaattgcta aaacagtatt aatgcctggc gatccgctac gcgcaaaata tattgctgat
      601 aattttttag aaaatgttga acaatttaac gatgtacgta acatgtttgg ttacactggt
      661 acatataaag gtaaagaagt ttctgtaatg ggttctggta tgggtattcc aagtattggt
      721 atttactcat atgagttata caacttcttt gatgtagata caatcattcg tatcggttct
      781 tgtggcgcat tacaagaaaa tgttaactta tacgatgtta ttattgcaca agctgcatca
      841 actaattcaa attatgtaga tcaatacaat attccaggtc atttcgcgcc tatcgctgac
      901 tttgagttag taactaaagc taaaaatgtc gctgaccaaa tcggtgctac tacacacgta
      961 ggtaacgtat tatcttctga tacattttac aatgccgatc caacattcaa tgatgcttgg
     1021 aaaaaaatgg gtattttagg tatcgaaatg gaatccgctg gtttatattt aaatgcgatt
     1081 catgctggta aaaaagcact tggtattttc acagtaagtg atcatatttt acgtgacgaa
     1141 gctactacac ctgaagaacg tcaaaattca tttacacaaa tgatggaaat cgctttagaa
     1201 atcgcagaat aacttattta aattgacttt aattgctctt taacaatgtg attaaactca
     1261 aaaagccaac acattctggg cgtatcccca tttatgtgtt ggcttttatt tatattatta
     1321 cttatctgta gattagctta agtaagattt aaacatccaa ttatgtttat ctactgatgt
     1381 ttgcatacct ataaacatat cttctgatac atcatcgcca gcattaccag caatttcaat
     1441 cgctttttct aattgttttg agatattagt gaaatcttga gataattctt caaccatttg
     1501 ttctgcagag taacctttcg cagcttcttt aacaattgat tgctctaagc attcagttaa
     1561 tgtacctaca gggtttcctc ctaccgctaa aattctttca gctaattcgt ctacatattg
     1621 acttgcttca ttatataatt cttcaaattt aacgtgtaat gagaagaagt taggcccttt
     1681 cacataccag tggaaattat gtagctttgt ataagctact gtccagtttg ctacttgttg
     1741 attcaattct tttacaacat cttgttgatt actcatattt aatacactcc ttaaaattgt
     1801 ctacgtcttg cttgtttata ttaattatta tattataatt attctaatct gtaaagtgtt
     1861 tttactctct tttttataat tattctaatt taattataag gtgtttcgct ttttaaatgc
     1921 ctaaatttta atccatcatc tagtatgtct attatcaatt agtaactatg ttttatcaat
     1981 cagtaagttt tactttcgca ttatcatcta aaaagaatga tttttcataa tcatttggtt
     2041 ggtgccaagt tgttttccac attctatttc tattattagc aaacattcga taaccgaaat
     2101 tacggactgg tttaggtaca agccataaac ctacgcccaa taatttagtt cgattaggta
     2161 gtgctgtgat tagttgaata atcgcctgcg attcaaaata tatacgatca cctttctgta
     2221 aaatcacact atttttattt gccgcttctg gttgttgttt aaaaaattgt tgtccaactt
     2281 caccttttaa cgtggcaaat tgataatttc ttggtaggtc atgttgaatt aa
//
LOCUS       DAPIDM010000214         2323 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000214 DAPIDM010000000
VERSION     DAPIDM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.221"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            2..130
                     /locus_tag="RJE42_002651"
     CDS             2..130
                     /locus_tag="RJE42_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878007.1"
                     /translation="MIKTMSIENMRTYQQRVYNLWIKGVDDVIYCGYQVGKQKIND"
     gene            complement(275..2203)
                     /locus_tag="RJE42_002652"
     CDS             complement(275..2203)
                     /locus_tag="RJE42_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDV5878008.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGYITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT          683 a          376 c          349 g          915 t
ORIGIN      
        1 tttgataaaa acaatgtcaa tagagaacat gcgtacttat caacaaagag tgtataactt
       61 gtggataaaa ggtgttgatg acgttattta ctgtggatat caggtgggaa aacagaaaat
      121 aaatgattga ttagtgttaa tttacttgtg gataactagg ataactctgt ggatagctat
      181 aatatcagcg tttttattgt gaataaaaaa atttaaaatt tgtaaaaaaa taactctaac
      241 tatagcgatt ttacagttag agttatgtat atttttatag tttttgttgt aattcttccc
      301 atttagacat agcgtgttct aatttttgtt cgctatcttg tttttggata gctaattcgt
      361 ttgctttttg tggattgtta taaacttctg gttgggttaa ttgctcgtct atttgtaaga
      421 tagtagtctc taaagtttct atttcatttt cacactcgga tatttgtcgt tcaagtttgc
      481 gttgttcacg tctttgttct ttttggttat gatacgacga ttgttccatg gacttttcat
      541 gtgcttgtac gttaactgac tgttcttctt ggtgcgcttt caacgcttcc gcttcctcaa
      601 ctttttcaat gtaatattga taatctccta aatacatctt tccgccatca attgttaaat
      661 caaatacctt gtttgctaat tggttaataa agtaacgatc atgggaaaca aatagtattg
      721 ttccttcaaa atgttggagt gcttgctcta acatttcttt tgaatctata tcgaggtggt
      781 tggtaggttc atctaaaata agtacattgt cacgttgcaa cataagtagt gctagttgta
      841 aacgtgcttt ttcaccacca gataaatcat taattatctt tttaacatcg tcttgtacaa
      901 ataagaaacg tccaagtact gcacgaatat ctttttcatt cattaacgga tattgatccc
      961 acacataatc taaaattgtt ttactagatt taaattctgc ttgcttttga tcataataac
     1021 caatttgtaa atttgcgcca aaagtaatat agccattaag cgctttttgt tgatttgcaa
     1081 tagttttaat taaggtcgat tttccaatac catttggccc aatgattgct atatgatcgc
     1141 ctttagagac ctctatactg ataggtttgg taattgcagt ttgataaccg atttctaaat
     1201 tttttacatg catgacgtca ttacctgtat tccggtcaaa gccaaattga atatttgcac
     1261 ttttggcatc taacattggt ttatcaatgc gttccatttt ttctaaaatc ttacgtctac
     1321 tttttgccat tccgcttgtt gaagcacggg taatattttt ctcaacaaaa gtttctaatc
     1381 gttttatttc tgcttgttga ctttcatatt cttgcattcg tttttgataa tataaatccc
     1441 gttgctgtat aaattcctcg taattaccaa catagcgttt gacatcaccc aaagccacat
     1501 cataaatttg agtaactatt ttatctaaaa agtaacgatc atggctgatg atgacgattg
     1561 cacctttaaa ataacgtaga taatcttcaa gccactttgt cgtttctaaa tctaagtggt
     1621 tagtaggttc atctaaaagt aataaatcag gttcatttaa tagcatttga gctaaagata
     1681 aacgtgtttt ttgaccaccg ctaaaatcat tgataggttt attgaaatct tcttcactaa
     1741 aatttaaccc gtggagtact gttttaattt tactttcata ttgatatcct tctaattgtt
     1801 caaattgatt cgataaagat tcataacggg acatatgtgt tttatatgta tcactgtcgt
     1861 aatcatttgc atgttttgat aaccaatctg tttcttcttt gattaagctt tccattcgtt
     1921 taatatgttc aaatggtttt gacatttctt caaaaactgt tgcattagaa tttaaagtca
     1981 tttgttgagt taaataacct agttttaagt ttttaatttt tgacacattt ccagaatcat
     2041 agttttctac gccagctata attttcatca atgttgattt accggcgcca tttcttccta
     2101 cgataccaat tcgttcacca gtttttactt caaaatcaac atcagtaaat atatcttcac
     2161 catcgaacga ttttgatata tgattgagtt gtaaaagtat cattcgatct tcacctttcg
     2221 tctttcgcaa attttattaa cttatgataa aagtcgtaat tattacatta ataaccattt
     2281 tacagtataa aacgccgtct tgaaactaag atatttattt tta
//
LOCUS       DAPIDM010000215         2297 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000215 DAPIDM010000000
VERSION     DAPIDM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.222"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            84..305
                     /locus_tag="RJE42_002653"
     CDS             84..305
                     /locus_tag="RJE42_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDV5878009.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            315..722
                     /locus_tag="RJE42_002654"
     CDS             315..722
                     /locus_tag="RJE42_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV5878010.1"
                     /translation="MEIEIKFNETFEAPMGSPRPRFRNTGRFVHTYMPTSYTKHKAYI
                     QGQMPKLNLEHALKIELDFYFPLLKSWSKKKKSEMVGQYKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGLEPKIIMRVEEVI"
     gene            722..907
                     /locus_tag="RJE42_002655"
     CDS             722..907
                     /locus_tag="RJE42_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDV5878011.1"
                     /translation="MQQQAYINATIDIRIPTEVDYQHFDDVDNEKEALADYLYNNPDE
                     LLEYDNLKIRNVNVEVE"
     gene            908..1525
                     /locus_tag="RJE42_002656"
     CDS             908..1525
                     /locus_tag="RJE42_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878012.1"
                     /translation="MAGIKTKVRIDGKLMTLIDVSDKYDIKVSTLITRYDRGARGKDL
                     IQNVVKPKKVKVDGKMMTVSELVKKYNLSKGLLNYRIAKGLTGDALIAPPQEKLPSKY
                     TEYENEQMKKKGLTPEIVRNRVAKGWEMSEAIDAPFGMKLNDYREIQITKALEREREM
                     ARQRRKEAELRRKKPHLFDVPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            1525..1779
                     /locus_tag="RJE42_002657"
     CDS             1525..1779
                     /locus_tag="RJE42_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDV5878013.1"
                     /translation="MSIISNRKVDMNEMQDNVKRPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWE"
     gene            1779..2027
                     /locus_tag="RJE42_002658"
     CDS             1779..2027
                     /locus_tag="RJE42_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878014.1"
                     /translation="MTMTDSGRKEYLKHFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQGVKKTFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            2042..2248
                     /locus_tag="RJE42_002659"
     CDS             2042..2248
                     /locus_tag="RJE42_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878015.1"
                     /translation="MKIKIEKEVNLPELIQWAWDNPKLSGNKRLYSNDVERNCCVTFD
                     VDSILCNVTGYVSINDKFTIQEEI"
BASE COUNT          898 a          349 c          496 g          554 t
ORIGIN      
        1 ccttttgatg attatgacaa agttgaagag aagatcataa atgaaatcaa ctggaagaaa
       61 acgcacatta aggagtgtta aagatgccga aagaaaaata ttacttatac cgagaagatg
      121 gcacagaaga tattaaggtc atcaagtata aagacaacgt aaatgaagtt tattcgctca
      181 caggagccca tttcagcgac gaaaagaaaa ttatgactga tagtgaccta aaacgattca
      241 aaggcgctca cgggctttta tatgagcaag aactaggatt gcaagcaacg atatttgata
      301 tttagaggtg gcacatggaa atagaaatta aatttaacga aacgtttgag gcgcctatgg
      361 gctcgcctcg tccacgcttt cgtaatacag gtagatttgt tcatacttac atgccaacgt
      421 cttacacaaa gcataaagcg tatatacaag ggcaaatgcc taaattgaat ctagagcatg
      481 cgctaaaaat cgaattagac ttttactttc cattgcttaa atcatggtcg aagaaaaaga
      541 aaagcgaaat ggttggacag tataaagtga ctaagccgga tatcgataac ttaattaaaa
      601 cagtattaga tgcttgtaat ggtcatgtat ggaaagatga caaccaaatt acagaaataa
      661 ctagctcaaa gcgttatgga ctagaaccaa aaataatcat gagagttgag gaagtgatct
      721 aatgcaacaa caagcatata taaacgcaac gattgatata aggataccta cagaagtaga
      781 ttatcagcat tttgatgatg tggataacga aaaagaagcg ctggcagatt acttatataa
      841 caatcctgac gaactactag agtatgacaa tttaaaaatt agaaatgtaa atgtagaggt
      901 ggaataaatg gcgggcataa aaacgaaagt gagaatagac ggtaagttga tgacgcttat
      961 tgatgtatcg gataagtacg atatcaaagt atcgacattg attactaggt acgacagagg
     1021 ggcgaggggg aaagatttaa tacaaaatgt agtaaagcct aagaaagtaa aggttgacgg
     1081 caaaatgatg actgttagcg aattagttaa aaagtacaac ctaagcaaag gactacttaa
     1141 ttacagaata gcaaaagggc taacgggcga tgcgcttatt gcgccaccac aagaaaaact
     1201 cccttctaaa tacactgaat atgaaaatga gcagatgaaa aagaaaggac tcacgccaga
     1261 aatagttaga aacagagtag caaagggttg ggagatgtca gaagcaattg atgcaccttt
     1321 cggcatgaag ctaaacgact atagagaaat acaaataaca aaagctttgg agcgagagcg
     1381 tgaaatggct aggcaacgac gtaaagaagc tgaactaaga agaaagaagc cacatttatt
     1441 tgatgtacca caaaaacatt cacgtgatcc gtactggttc gatgtcactt ataaccaaat
     1501 gttcaagaaa tggagtgaag cataatgagc atcatcagta acagaaaagt agatatgaat
     1561 gaaatgcaag acaacgttaa gcgaccagcg cactacacat acggcgatat tgaaattata
     1621 gattttatcg aacaagttac ggcacagtac ccaccacaat tagcattcgc aataggtaat
     1681 gcaattaaat acttgtctag agcaccgtta aagaatggtc atgaggattt agcaaaggcg
     1741 aagttttacg tcgatagagt atttgacttg tgggagtgat gaccatgaca gatagcggac
     1801 gtaaagaata cttaaaacat tttttcggct ctaagagata tctgtatcag gataacgaac
     1861 gagtggcaca tatccatgta gtaaatggca cttattactt tcacggacat atcgtaccag
     1921 gttggcaagg tgtgaaaaag acatttgata cagcggaaga gcttgaaaca tatataaagc
     1981 aacatggttt ggaatacgag gagcagaagc aactaacttt attttagagg agatggaaat
     2041 gatgaaaatc aaaattgaaa aagaagtgaa cttgcctgaa cttatccaat gggcttggga
     2101 taaccccaag ttatcaggta ataaaagact ctattcaaac gatgttgaac gcaactgttg
     2161 tgtgactttt gatgttgata gcatcttatg taatgtgact ggatacgtat caattaacga
     2221 taaattcact attcaagagg agatataaca atgaaaatca aagtaaaaaa agaaatgaga
     2281 ctagatgaat taattaa
//
LOCUS       DAPIDM010000216         2166 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000216 DAPIDM010000000
VERSION     DAPIDM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.223"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(1..1997)
                     /locus_tag="RJE42_002660"
                     /pseudo
     CDS             complement(1..1997)
                     /locus_tag="RJE42_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
BASE COUNT          549 a          435 c          322 g          860 t
ORIGIN      
        1 tcaatcacgc cataattacc gtgaccattt tcagttccta aaccagaatg agaaatatga
       61 tgattgtttt cagtaatttc ctcgattggc ccttgtgctt gaccatgttc ttcaggtaat
      121 tcatccacta attcaatcag attactttca gttgtatatt ctttcgtatc ttcaactgtt
      181 gtatggtcgc tcactgcgcc agttacaata ccttttgtag actcttcgtc aaattcaact
      241 aagttagact cagttgttac ttggccacca cctgggtttg tatcttcttc atattcaaca
      301 acatcagcat gatgttttga attttcatgt gtcgattctt caaagtctac atgaatagaa
      361 tcttcttcag tttcaatggt accttctgca tgaccttctg caccttcaac agctgtatga
      421 tattcaaaat caattggctt agaatcgtca ctttcttcga ctgtaccagt caattcatgc
      481 ttctccattg gcggctctga tttaaattca agttcgatag gagtactatg ttctataata
      541 ggttcctttg gtttatcttt accgtcgccc tgagcgttat tagagtaaaa tgcaacgcca
      601 ttgttccaag ttagttgaac tggataatat gaataataat aattatgata accggataac
      661 ttcgtttgga agtttaaatc actagcagac gagttatagt caccttcata cttaattaca
      721 taatttttag ttttatccaa ttcactaaaa tctaaagtat atccaccatt tccagtaagt
      781 gttaaactta gtttatcagt tacatcttca aactttgttt catccagaac atctccataa
      841 acactttcag ctaattcatc cgaaccaatg tgtttatata cttagactgt aggattctta
      901 gcattttgtt tatatccaga tgttacttga ccagttactg tgacagaagt taacgactgg
      961 ttgttaggct tcacgtatgc aaaatggcta aatctacctt cttgcttatt caaagtatga
     1021 attcgaccat taactgttac tccccagtta tcttttacac cgtctaagta ttttatgtcg
     1081 aattgtttag atatcttctt ctctcctaag gtcacttcaa tattttggtt acctcttttt
     1141 gtcactgttg ttgggtcgat aaataggttt aaatctaact cagcttttaa atctttttta
     1201 ccttgtacat attctttaaa tgtataccta attttccttt cttcattcac ttcacctact
     1261 gccataactt gaccatcttt actttttata tctggcactt tacgaagagg tgagatacca
     1321 tgcgtttcta cattattact taatgtgaaa tcaaagtagt cccccggttt aataccatcc
     1381 tcgaatttcc atttatattt caaggttact ctttctgcat tatgaggatt aacaacattt
     1441 gtctctttat tgttatgccc tactatttca ctaccttctt ctactttcac tttacttgtt
     1501 acatctgtac ctgtcacctt agcttcttcc actacttctt tctctgaagc tgctgtaacg
     1561 tcagctgatc ttttcattct tggtttaatt tctgagacgt tacttggttg agttgtgtca
     1621 acttgagttc ccactattcc cttatcagca gctttttccg atggcaaatc aactcgcgaa
     1681 gtttctgctt ttggtgcttg cacagttgtt ggtgcttctt ctgttgttac ttgtgttgat
     1741 tgtgatggtt gctcagttga tgtcgcgctg tatgattgtg tttcatctat tgtattaacg
     1801 ttatttgtag ttgtttgtgt ttcgcttgct ttactttcag tagctgaatt cccactttcc
     1861 tctactgtag tattgttttg ttccgatgct gcggcttctt tttcttgtcc cattccaaca
     1921 acgatcattg ttcctaagaa tactgatgcc gctcccaatt tgtgttttct tatgccgtat
     1981 ctaagattgc ttttcactat aatattctcc cttaaatgta aaattcattt attttttaac
     2041 tcaataaatg caattctata ttgttcggtt tttaaaagca atgaaaaaaa gcgagttaat
     2101 aaaaagttaa gattgttgtt aactttatgt ataatgagtt tttattattt ggaactcaca
     2161 tatata
//
LOCUS       DAPIDM010000217         2151 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000217 DAPIDM010000000
VERSION     DAPIDM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.224"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..779)
                     /locus_tag="RJE42_002661"
     CDS             complement(<1..779)
                     /locus_tag="RJE42_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV5878016.1"
                     /translation="MTNLNNQLKEFKNTIKVERSKLLPSPFDMVYKNFEKLGYHKQNE
                     QFFYDNASIIIDELRNQSWIEFKKIEKEFSKKLYSHLLDQDSENYKNMTVKEGIEKFT
                     SKHTDEIYALQLSNTQSRRSRAGKEFESIIELVLMGANIEFVTQGSIGSGVFESSELA
                     KLVDCVVPGAAEYNLNKRNTSLISTKTSLRERWQEVGDEMSRTKAKEMYLVTLDESIS
                     EKTLKLIEKNNIIIVTTKSVIDKNYLNSSNVISFEDMLNEL"
     gene            875..2068
                     /locus_tag="RJE42_002662"
     CDS             875..2068
                     /locus_tag="RJE42_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDV5878017.1"
                     /translation="MYNNIITKNESDNSIPLNYGDFIKEKRNKLNMTQKELADSIGLP
                     KFGDRTIRRWENYETSPSYLEYKTIMNFPDTPPYLNPNIYDYKMIDLFAGIGGTRLGF
                     QLHGNVKCVFSSEWDKFSAKTYKANFGETPKGDITQIIADEIPTHDILVAGFPCQAFS
                     QAGLKKGFNDTRGTLFFDIARILKAKRPKCFLLENVKNLRSHDKGKTFDIIMNTLLEL
                     DYDVSSSILRARDFGCPQNRERIYIVGFDKQQIKNAENFSFPEPLPIETSLGSILESN
                     VNDKYTISDRLWYGHQRRKREHKAKGNGFGYSIFNETSPYTNTLSARYYKDGSEILIE
                     QNKKNPRKLTPREAARLQGFPENYIIPVSDTQAYKQFGNSVSVPVVHAIASNIFQVLQ
                     QNKNN"
BASE COUNT          735 a          348 c          299 g          769 t
ORIGIN      
        1 tgtaattcat ttaacatatc ttcaaaacta ataacatttg aactatttag ataatttttg
       61 tcaataacag actttgtagt tacgataatt atattatttt tttctatcag ctttaatgtt
      121 ttttcactta tactttcatc taaggttacc aaatacattt ctttagcttt agtcctagac
      181 atctcatcgc cgacttcttg ccatctttct ctcaaactgg tctttgtgct aattaaactg
      241 gtatttcttt tatttaagtt atattctgca gctccaggca ctacacaatc aactaatttt
      301 gctagttctg aagattcaaa aactccgctc ccaatcgaac cttgagttac aaattcaatg
      361 ttggctccca ttaacaccaa ttctattatt gattcaaatt cttttccagc tctggatctt
      421 ctactttgtg tattagataa ttgtaaagca tatatttcgt cagtatgttt agatgtgaat
      481 ttttcaatcc cttctttaac agtcatgttt ttataatttt cactatcttg atctaataga
      541 tgagaataaa gctttttcga aaactctttc tctatttttt taaattcaat ccagctctga
      601 tttctaagtt catcaataat tatactggca ttatcgtaga aaaattgctc attctgtttg
      661 tgatatccta atttttcaaa gtttttatat accatatcaa atggacttgg aagtaattta
      721 gatctctcaa cctttatagt atttttaaat tcttttaatt ggttattcaa attagtcatc
      781 ccttcatttc atttgactta tactcttttt aaatataaaa tgtgtcaatg aaaggacatt
      841 acgtcctatc atcatcacaa aggaagcgtt ttaaatgtat aataatatta taacaaaaaa
      901 tgaaagcgat aatagtatcc ctttaaatta tggtgatttt attaaagaaa aacgcaataa
      961 actcaacatg actcaaaagg aattagcaga ttctataggt ttacctaaat ttggcgatag
     1021 aacaattcgc agatgggaaa attatgaaac atcaccttca tatctagaat ataaaacaat
     1081 tatgaacttc ccagataccc caccttattt aaatcctaat atttatgatt ataaaatgat
     1141 tgatcttttt gctggtattg gaggaacaag attaggattt caattacatg gcaatgttaa
     1201 atgtgttttt agtagtgagt gggataaatt ttctgcaaaa acttacaaag caaattttgg
     1261 tgaaactccc aaaggtgata taactcaaat cattgcagat gaaataccta cccatgatat
     1321 attagttgct ggatttcctt gtcaagcttt ttcccaagca ggtttaaaaa aagggtttaa
     1381 cgacactaga ggcactcttt tctttgatat tgcacgaatt ttaaaggcaa agagaccaaa
     1441 atgtttttta cttgaaaacg taaaaaattt acgctcccat gataaaggaa aaaccttcga
     1501 tattataatg aatactttgt tagagttgga ttacgatgta tcatcatcaa tacttagagc
     1561 gagagatttt ggctgtccac aaaatagaga acgaatttat atagttggtt ttgataagca
     1621 acagattaaa aatgccgaaa atttttcatt ccctgaacca ttaccaatag aaacttcttt
     1681 aggtagtata cttgaaagca atgtaaatga taagtataca atatcagata gactttggta
     1741 tggtcatcaa agaagaaaaa gagaacataa agctaaaggt aatggatttg gatattcaat
     1801 atttaatgag acaagccctt atacaaacac cttatctgca agatattaca aagatggtag
     1861 tgaaattttg attgagcaaa ataaaaaaaa tcccagaaaa ttaacacctc gagaagctgc
     1921 acgactacaa ggatttcctg aaaactatat aattcctgta agcgatactc aagcttataa
     1981 acagtttggc aattctgttt ctgtacccgt tgtacacgct atagcttcta atattttcca
     2041 agtcttacaa caaaataaga ataattaatg ctttgctttt aaataagttt atttagccta
     2101 tcacaatgat acctgctttc cgatgttctt tttgtgatag gctgtgtttt t
//
LOCUS       DAPIDM010000218         2146 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000218 DAPIDM010000000
VERSION     DAPIDM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.225"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(1058..1522)
                     /locus_tag="RJE42_002663"
     CDS             complement(1058..1522)
                     /locus_tag="RJE42_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878018.1"
                     /translation="MKRLLGLLLASTLVLVACGNDENKEESKKEVKSKEKKIEKEKEN
                     KSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQLPQNSVPQQNAQVQQNKKQKV
                     DLNNMPPTDFSTEGMSEQAQKQIEELTMQKDYHGLSQREYNDRVSEIINNDN"
BASE COUNT          667 a          308 c          287 g          884 t
ORIGIN      
        1 ttttaagcgc tatttttatt ttcgactcaa acccttgata tatctgcatt tgatctatta
       61 tcctcatttt ttacacttct tatttattta tatccattta aaatttttta gccactatgt
      121 gactaatttt tgatgattaa tcttaatttt agtcacaaga ttttgaagtt tagtcacaaa
      181 agaaatcatt aagatttttt tctataaatt aagtttcatt ttcttcaaat tctttaatca
      241 aatgcgaata tgttttaagc gttgtatgta tatctgcatg acctaatcgt tttgatatgt
      301 aatgtatagg tgttccttaa agaaaatcga atattatata ttgaaaaatt aagataaaaa
      361 agttataata taatcaatcg gcatctacgt gcggatttta aagaaaagcg tagcataacc
      421 aggagttagt caatttttaa taaattcgac taaattatta ataaaggcta aagtaattag
      481 tttactttag cctttattgc atttgtaata tatagaactg cgaacagggg actactatta
      541 atttatgtga ttaaagtttc aatgatattt gatatattag accatttcaa aatcgcaagg
      601 ttctcatgtt ctgctacagt ttgcgctctt tggaagatat atatcatata ctattactaa
      661 catgataggt tataaattat ttctggatgg actggtaggt ataatatgtc gatatatata
      721 agctttatta gttattttgt tgaaatctaa gttgaattta atattttaaa aaagtgcaaa
      781 tcaatgatca ttattatgaa attctttagg tgtgtaactt aatattagaa tccgccgatt
      841 tatgatgtct tatcaacaat aaaaaaactt ttgattaatt tagtgtgtac tataaatttt
      901 acttaagttg cttcgcgttt agttaaaaaa gttcctttta taacgtttac ttttatggtc
      961 aaagtcataa taacgtttat ctttatcaaa tctactgata aacttattat tactgaaatt
     1021 tgtcaaaaaa taagggtagg agggctaccc tgtggaatta attatcatta tttataattt
     1081 cagaaactct atcattgtat tctctttgtg acagaccatg atagtctttt tgcattgtaa
     1141 gctcttcaat ttgtttttga gcctgctcag acataccctc tgtagaaaaa tcagtgggag
     1201 gcatattatt taaatcaact ttttgctttt tgttttgttg aacttgggca ttttgctgag
     1261 gaacactatt ttgtggtaac tgttgttgcg gttgttgaac cgattgctct tgtgattggg
     1321 gttgttcaac ggtttgatta tctggttgtt gttgtgttgc aacttctttt tccttgtctt
     1381 ttttcgattt attttccttt tccttctcaa ttttcttttc ttttgattta acttcttttt
     1441 tagattcttc cttattctca tcatttccgc atgcgactag cactaacgta ctagctaata
     1501 ataaacctaa taatctcttc attttcattt ctcctatgtt tatattatat taaatcacca
     1561 tataggcgcg attaattata gatatgatag ctataaataa ctttacctat cactttgatt
     1621 tcatcaatag aattttaatc gtaagtatta gattttaaat tcacttgaat aatctatgtg
     1681 tttatatgtc attttgtttc agagcgtttt acacattcaa ctgtatattt gcgaccttta
     1741 cgcaatacga gcatatcatt gctattaaat ctatcatcac tagaattaat ataataatga
     1801 actacaatgt gagaaccggt aacaaggatt ttattcatat ctttctggtt ctagaccctt
     1861 ctctcttaat gcttgatctc tactttcttg tgctctttaa tctccttcag atttatagaa
     1921 acgattatac tctctgtgtc tgtttctcac actacatatc tatctataat agaattgttt
     1981 atatctgagt gagattaaac actataactt tctataactg agtaattatt tttactgcac
     2041 tttagttata agtaaatcta tatttcaaaa attaaacaag ttctgaaatc atagtattgt
     2101 ccaattaagt tcagacaata cattcaatta attataaaat aaacaa
//
LOCUS       DAPIDM010000219         2140 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000219 DAPIDM010000000
VERSION     DAPIDM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.226"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..825
                     /locus_tag="RJE42_002664"
     CDS             <1..825
                     /locus_tag="RJE42_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV5878019.1"
                     /translation="DGTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWH
                     EGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLN
                     QRTYLWVELGLQTIHQSTSDLINRAHDMQTYYDGVAKLRKHNINVCTHIINGLPGEDY
                     DMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEV
                     IPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            822..1385
                     /locus_tag="RJE42_002665"
     CDS             822..1385
                     /locus_tag="RJE42_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV5878020.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNYVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAIKALLSLMSTEGIIVLVIYHGHSEGQLEKQALLDYLSNLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1504..1905)
                     /locus_tag="RJE42_002666"
     CDS             complement(1504..1905)
                     /locus_tag="RJE42_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV5878021.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
BASE COUNT          751 a          362 c          345 g          682 t
ORIGIN      
        1 gatggcactg tagcacatgg tggatgtaca ttttgttctg ctgcaggtag cggagacttt
       61 gcaggtaatc gtgcagattc aatcgcagta caatttaaag aaattaagga aaagatgcat
      121 gagaaatggc acgaaggaaa atatattgct tattttcagg catttacaaa tacacatgca
      181 ccagttgaag tattaaaaga aaaattcgaa cctgtactta aagaaccggg tgttgtggga
      241 ttatctattg gtacgcgtcc tgactgtcta ccagacgatg ttgtcgaata tttagcagat
      301 ttgaatcaac gaacatactt atgggttgaa ttaggactac aaacaatcca tcaatcaaca
      361 tctgatttaa tcaatcgtgc ccatgatatg caaacgtatt atgatggtgt ggcaaaatta
      421 cgtaagcata atatcaatgt atgtacacac atcattaatg gcttacctgg cgaagactat
      481 gacatgatga tggctactgc caaagaagtt gcacaaatgg atgtacaagg tattaaaatt
      541 catttacttc atttgttaaa aggtacaccg atggtaaaac aatacgataa aggtttatta
      601 acttttatga ctcaagaaga atacacaaac ctagttgtgg accaattaga agtgattccc
      661 cctgaaatga tcgttcaccg aattaccggt gatggtccaa tagatatcat ggtcggtcca
      721 atgtggagtg ttaataaatg ggaagtatta aatggcatcg atgctgaatt agcacgtaga
      781 aattcttatc aaggcttgcg ttacaagtct aaggtgaagc aatgaaatta gaacgtatac
      841 tccctttttc aaaaacactt attaaacaac atataacacc agaaagtatt gttgtagacg
      901 caacttgcgg taacggcaat gacactttat ttttagccga acaagtacca gaaggacatg
      961 tttatggttt cgacattcaa gatttagctt tggaaaatac acgtgataaa gttaaggatt
     1021 tcaattatgt ttctttaata aaagatggac atgaaaatat tgaacatcat ataaatgatg
     1081 cacataaagg tcatattgat gcagccatct ttaacctagg ttatttgcct aaaggtgata
     1141 aatctatcgt gacaaagcct gacacgacaa tccaagctat taaagcgtta ttatctttaa
     1201 tgtcaacgga aggtattatc gtattagtta tctatcatgg tcatagtgaa ggccaacttg
     1261 aaaagcaagc tttgcttgat tacttgagta atttggatca aaagcatgcg caagttttgc
     1321 agtatcaatt tttaaatcaa cgaaatcatg ctccattcat ttgtgccata gaaaaaattt
     1381 cttaagtatt tgcttcacta ttctatttat aatttagatt gagtagtcaa catttgaaaa
     1441 attaatacca acaacaaaaa gaggttttca ttaagttgaa aacctctttt tatgctatta
     1501 aacttacaca gcaataattg cgtttaaact attttgcatt gctgttgctc tacttgcaat
     1561 cgcatcactg atgaaattca acaactctac tttctcttgt tgtaactttt cattgaaatg
     1621 aataataact gttctttcat cgtcaacagg acgctctttg taaatccatt ctaattcaac
     1681 taaattatta tacgttctag tacgcttata cggtttaact tcaacaaatc tgtccatttc
     1741 tttaagcgtc atagaacctt tttgccataa agttagtaaa attaaaattt cttctctaga
     1801 cattttgtac tcgctttcaa tctcgctgaa aattgagtta atgtcaccca aaagtgtttc
     1861 taattgcaaa atcccaaata cagtatcgtt atttactttt ttcatgctat acatctccca
     1921 attaataatc acatatttag ctaccataca agtattaaca aacatcccaa caatcccaaa
     1981 acttatatgt gctaacttat gcataaaact acaagtgtaa ataaacttgc tttctattca
     2041 attgtctaag ttgaataaaa ttaaataagt ataaaaacca ataatcacta gagttaatat
     2101 ttagaaataa gataatagta cataaaacaa atcactctat
//
LOCUS       DAPIDM010000220         2103 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000220 DAPIDM010000000
VERSION     DAPIDM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.227"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            263..1189
                     /gene="emp"
                     /locus_tag="RJE42_002667"
     CDS             263..1189
                     /gene="emp"
                     /locus_tag="RJE42_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDV5878022.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKNFVASEDKLN
                     KIADPSAASKIVDKNFAVPESKLGNIVPEYKEINNRVNVTTNNPASKQVDKQIVVKGP
                     EVNRFITQNKVNHHFITTQTHYKKVITSYKSTHVHNHVDHATSSIHHHFTIKPTEAPR
                     YTHPSQSQSLIINHHFAVPGYHGHKVVTPGQASIRIHHFCAVPQINSFKVIPSYGHNS
                     HRMHVPSFQNNTTATHQNAKVNKTYNYKYFYTYKVVKGVKKHFSFSKSHGCKIVKPAL
                     NIKNVNYQYAVPSNSPTHVVPEFKGILPAPRV"
     gene            1523..2005
                     /locus_tag="RJE42_002668"
     CDS             1523..2005
                     /locus_tag="RJE42_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878023.1"
                     /translation="MKRKVLVLTMGVLCATQLWQTNNAKALVHESGVNDTKQFTEVTS
                     EEKVITVEHAYINIFKSNSNSNLMEFNILTIGGKSGAMVGYSEIDSSHFTDRDKRAIR
                     RDHVKEAQSLINDYKDTQSYEDLAKATAKVSTLSQSHQNYLNKQIDKVNNKIEKTEKR
                     "
BASE COUNT          842 a          329 c          304 g          628 t
ORIGIN      
        1 tcaatgatgc aatctagtat agtccacatt ctaaacaggt gtagactatt acttttttca
       61 ctttatatta cgaaaaaatt attatgctta actatcaata tcaataatta attttaagct
      121 gaaaaacaat aaaaatgtta agataacgtt tacttcaagt taattattat actgaaaatt
      181 ctggtatata atgctgttag tgaatataac aggaaaatta aattggttat aatattgagt
      241 ctatataaag gagaaataac agatgaaaaa gaaattatta gttttaacta tgagcacgct
      301 atttgctaca caaattatga attcaaatca cgctaaagca tcagtgacag agagtgttga
      361 caaaaacttt gtagcttctg aagataaatt gaataagatt gcagatccat cggcagctag
      421 taaaattgta gataaaaact ttgccgtacc agaatcaaaa ttaggaaaca ttgtaccaga
      481 gtataaagaa atcaataatc gagtgaatgt aacaacaaac aatccagctt caaaacaagt
      541 tgacaagcaa attgttgtta aaggcccaga ggtgaataga tttattacgc aaaacaaagt
      601 aaaccaccac ttcatcacta cgcaaacaca ctacaagaaa gttattactt catacaaatc
      661 aacgcatgta cataatcatg tagaccatgc aacatcttct atccatcatc actttactat
      721 taaaccaaca gaagcaccta gatatacaca cccatctcaa tctcaatcgt taattataaa
      781 tcatcatttt gcagttcctg gataccatgg tcataaagtt gtaacaccag gacaagctag
      841 tattagaatt catcactttt gtgctgtacc tcaaataaat agttttaagg tcattccatc
      901 atatggtcac aattcacatc gtatgcatgt accaagtttc caaaataaca caacagcaac
      961 acatcaaaat gcaaaagtaa ataaaactta taactataaa tatttttata cttataaagt
     1021 agtcaaaggt gtaaaaaaac atttctcatt ttcaaaatca catggttgta aaattgttaa
     1081 accagcatta aacatcaaaa atgtaaatta tcaatatgct gttccaagta atagccctac
     1141 acacgttgtt cctgagttta agggcatctt accagcacca cgagtataaa aattgacatt
     1201 aagtttacga gatatgataa atacctatta ttttaaatat agtctgcaat ctatgaggtt
     1261 gtaggcaatg tttttgctgt ttaacgatca acacccatca acaaattata ccgtttttct
     1321 actttaaaag ttggaagtaa cataatctta aataaatata ttattaatta agataaatat
     1381 aagactcaag attattgtta atagtttgtt catcgcaagt taattattgt ttctaaaata
     1441 ttggtatata attttcaatg gcgaagaaaa cagggtgaaa aagtcggttt ttaaatcaaa
     1501 gcaaataagg gagcttaaac aattgaaaag gaaagtacta gtattaacaa tgggcgtact
     1561 ttgtgcgaca caattatggc aaacgaataa tgcaaaagct ttagtgcatg agagtggcgt
     1621 taatgatact aagcaattta ctgaagtaac atcggaagaa aaagttataa cagttgaaca
     1681 tgcttatatt aatattttta aatctaatag caattcaaac ctgatggagt tcaacatatt
     1741 aacaataggc ggtaaatcag gagctatggt tggttatagt gaaattgact catcacattt
     1801 cacagaccgt gacaaacgcg ctattagacg tgatcatgtt aaagaagcac aaagcttaat
     1861 taacgattat aaagatacac aaagctatga agatctcgct aaggcaactg caaaagtaag
     1921 tacacttagt cagtctcacc aaaattattt aaataaacaa attgataaag tgaataataa
     1981 gatagagaaa actgaaaagc gctaatccaa agtaaattat aagttataca tctcgttttt
     2041 aaatgacaat ttatccccgt aaatataata aataatcttt tcacattccg catagatata
     2101 gag
//
LOCUS       DAPIDM010000221         2087 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000221 DAPIDM010000000
VERSION     DAPIDM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.228"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            159..251
                     /locus_tag="RJE42_002669"
     CDS             159..251
                     /locus_tag="RJE42_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878024.1"
                     /translation="MDNDKVLETSKEMYVEQKCLIFYKTLKEYV"
     gene            304..1791
                     /gene="lysS"
                     /locus_tag="RJE42_002670"
     CDS             304..1791
                     /gene="lysS"
                     /locus_tag="RJE42_002670"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV5878025.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
BASE COUNT          751 a          259 c          422 g          655 t
ORIGIN      
        1 gttgtattaa tgataatcgt attttaaatg aataagtaat tcataaacga aaacagaaat
       61 tgctttatta catttacaac ctcttgtaaa attaaaaaag tgataaaatt gatgttatga
      121 atataagaaa acatgccaca tcataacttt aaggtgcaat ggataatgat aaagtattag
      181 aaacatcgaa agagatgtat gttgagcaaa aatgtctgat attttataaa actttaaagg
      241 aatatgtttg agtgtaccag ttggaatact aaaggattac aacaagttaa aggagagaaa
      301 gttatgtcag aagaaatgaa tgaccaaatg ttggttcgac gtcaaaaatt acaagaatta
      361 tatgatcttg gtatagaccc gtttggttct aaatttgacc gttcaggttt atctagtgat
      421 ttgaaagaag agtgggacca gtattctaaa gaagaattgg tagaaaaaga agcggatagt
      481 catgtcgcta tagctggacg attaatgact aagcgtggta aaggtaaagc aggatttgca
      541 cacgttcagg acttagctgg acaaattcaa atatacgttc gtaaagatca agttggcgat
      601 gacgaatttg atttatggaa aaatgctgat ttaggcgata tcgttggtgt tgaaggtgta
      661 atgttcaaaa caaatactgg cgaattatcg gttaaagcga agaaattcac gctactaact
      721 aaatcattgc gaccattacc ggataaattc cacggtttac aggatatcga acagagatat
      781 cgtcaaagat atttagattt aattacgaac gaagatagca ctcgtacatt tattaatcgt
      841 agtaaaatca ttcaagaaat gcgtaattat ttaaataata aaggtttctt ggaagtagaa
      901 acacctatga tgcaccaaat tgctggtggc gcagctgcta gaccatttgt aacacatcat
      961 aatgcattag atgcaacgtt atacatgcgt attgctattg agttgcattt aaaacgttta
     1021 attgtcggtg gacttgaaaa agtatatgaa attggtagag tattccgtaa tgaaggtgta
     1081 tcaactagac ataatcctga attcacaatg attgaattat atgaagcata tgcagattat
     1141 catgacatta tggatttaac agaatctatg gtgagacata ttgccaatga agtattaggt
     1201 tctgcaaaag tacaatacaa tggggaaacg attgatttag aatctgcttg gactcgtttg
     1261 catattgttg atgctgtaaa agaagctact ggtgtagatt tttatgaagt taaaagtgat
     1321 gaagaagcta aagctttagc taaggaacat ggtattgaaa ttaaagatac aatgaaatat
     1381 ggtcatattt taaatgaatt ctttgagcaa aaagttgaag aaacacttat tcagccaacg
     1441 tttatctatg gtcatccgac tgaaatttca cctttagcga agaaaaatcc tgaagatcct
     1501 agatttactg atcgtttcga attgttcatt gtaggtagag agcatgcaaa tgcatttact
     1561 gaattaaatg atcctattga tcaaaaaggt cgctttgaag cgcaacttgt tgaaaaagcg
     1621 caaggtaatg atgaagcgca tgaaatggat gaagattaca ttgaagcgtt agaatatggc
     1681 atgcctccga caggtggtct tggtatcggt attgacagat tagttatgtt attaactgac
     1741 tctccatcga tcagagacgt attattattc ccttatatga gacaaaaata aatgacgttg
     1801 attgttagta agagctctcg tgtatacaac atgtgtatgc gagggttttc ttaattatgg
     1861 taattagttc gtgtttgaat gtttttgata gtaatagtta acgataatgg tgctattttt
     1921 gactgttaaa caaggtagtt agctgttaga tgaaaatgac gatacgtata tatattagtt
     1981 ctgatgagaa atgttaatga tagacattga gatatcttat aacaaaaatt gctatattag
     2041 tataatttat cttaatcagc tataaaagta ctttaaaatt gtataga
//
LOCUS       DAPIDM010000222         2055 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000222 DAPIDM010000000
VERSION     DAPIDM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.229"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(192..395)
                     /locus_tag="RJE42_002671"
                     /pseudo
     CDS             complement(192..395)
                     /locus_tag="RJE42_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(593..718)
                     /locus_tag="RJE42_002672"
                     /pseudo
     CDS             complement(593..718)
                     /locus_tag="RJE42_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(961..1404)
                     /locus_tag="RJE42_002673"
     CDS             complement(961..1404)
                     /locus_tag="RJE42_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5878026.1"
                     /translation="MKKKYILKVVVVIIIGLVVVACVTHKHRKDYYIETQEKRIDLYF
                     KYNLNNYSSMKVTSFKKNPMGGYFIKGFVNNKNNYKFDAVIFSDTNKQFKGDLGYKED
                     KIGELFREKDAKDRLNVDEIIEKEHLDKSDYVAEPPLFFFSGRLE"
     gene            complement(1404..1847)
                     /locus_tag="RJE42_002674"
     CDS             complement(1404..1847)
                     /locus_tag="RJE42_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDV5878027.1"
                     /translation="MKKRYVFVVVVAILIGLIVIAYAHNKQIKAHYIKTQEKRIDLYF
                     KHNLNNYKNMKVTDFHKTPMGGYFIVGYINDDKKYKFQASIDSGSNNQYQKDIGYHED
                     KLGKLFKEKDPKYKLSVDEIIEKEHLDKSEYEADPPLFFFSGRLE"
BASE COUNT          633 a          357 c          222 g          843 t
ORIGIN      
        1 tgcttgagaa tggtctaact tatcttcagc atgagctaca tctgaattat aagataatga
       61 aattggacta acaaataata caccagcagc taataataaa gatttttaat tcgtttttca
      121 ttagtttctt tttgatgatt tttgtattta gctttcgtat aaacagaaac tagattatgt
      181 tttatcatga tacgacctat ctttcgtctt gatacagtga gacccttatc atttaaatta
      241 ttttcaattc gtcttgtacc aaagaccttt ctattagaat taaaaatatt tatgacggca
      301 cgttctatgt ttgaatcatc tttagtgatt ttattatctt ttctttttat agaatcataa
      361 taagtacttc ttagtatttt taggacttta cacattgctg gtactgaata ttgatgtgca
      421 ttcttttgaa tgacttctat ttttgtccca taatcagcgc tgcttgcttt aaaatatcgt
      481 tctccatttt taaatgttga acttctttac gtaatttaat cagctctttt tcttcatctg
      541 ataagttatc atggtgattg aatgtacccg tgttttgatg ttgctttatc cattttccta
      601 acgccgaagg tgttaaatca tactcgcgta taatttcatt tctaggctta ccattttcat
      661 ataatctaac catttgtaac ttaaactctg aactaaatga tcttctttct cttgtcataa
      721 taaaatcgcc tactttctta aattaacaat atctattctc atagaatttg tccaattaag
      781 tgtagacgat tcaaactttc atagagctat aatgattaag gttatactta aaatataaat
      841 ctattctctt ttcttgcgtc tcaatataat gatctttttt atgcttatgg atcacacatg
      901 ccacgactag caaacctatt ataattacta caactattat taaaatatat ttctttttca
      961 tcactctaat ctccccgaaa agaagaataa tggtggttcc gctacataat cacttttatc
     1021 caaatgttct ttttcaatta tttcatcaac atttaatctg tctttagcat ctttttctct
     1081 aaataattca cctattttat cctctttata acctaaatct cctttaaatt gtttattagt
     1141 atcagaaaat atcacagcat caaatttata attattttta ttattgacaa agccctttat
     1201 aaaataacca cccattggat tctttttaaa acttgtaact ttcatagaac tataattatt
     1261 aaggttatac ttaaaataca aatctatacg cttttcttgc gtctcaatgt aataatcttt
     1321 tctatgttta tgagtcacac atgccacgac taccaaacct attataatta ctacaactac
     1381 ttttaagata tatttctttt tcatcactct aatctccccg aaaagaagaa taacggtgga
     1441 tcggcttcat attcactttt gtctaagtgt tctttttcaa ttatttcatc gactgagagt
     1501 ttatatttag ggtctttttc tttgaataat ttgcctaatt tatcttcatg gtaaccaata
     1561 tctttctgat attgattatt gcttcccgaa tctatacttg cttggaattt atatttttta
     1621 tcatcattta tataacctac aatgaaatac ccacccatcg gagttttatg gaaatctgtc
     1681 actttcatat ttttataatt gttaagatta tgcttaaaat acaaatcgat tcgtttttct
     1741 tgtgtcttaa tgtaatgagc ctttatttgt ttattatgcg cataagcaat aacaattaga
     1801 cctattaata ttgcaacaac taccacaaat acatatctct ttttcatttt aaccccctag
     1861 tgtagtattt atttcttata gattttaaat ttcaaaaaaa caatttactt tgacatgcta
     1921 tcttttttca atgttccgta tacgccctca agaaaagttg tttcaaaata cttttaataa
     1981 ttactcgctt tacatcgtaa taaatacata aagtcgcgca gttttgcatt tcgcattata
     2041 tataaaatag caatc
//
LOCUS       DAPIDM010000223         1969 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000223 DAPIDM010000000
VERSION     DAPIDM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.230"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            137..1810
                     /locus_tag="RJE42_002675"
     CDS             137..1810
                     /locus_tag="RJE42_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDV5878028.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNWPDSWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVLVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDETMLPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNK"
BASE COUNT          697 a          329 c          298 g          645 t
ORIGIN      
        1 ttatttttca ttttaaatct gttcatcaaa atgaattgta cttaatgtaa tgaagtaact
       61 tatactattc atgaatttta cataagaccg tgctaaaata aggaagatac tacgataaga
      121 aatggaagtt atagcaatga ttaaacagat aaatgtttct aatatgcaaa agtttgaatc
      181 tcaattaatg aaagctcagt ctgaaggtta tacacatgtc gttccatatg ccaatgagat
      241 aatgatttat caatctatgt tagatgcggt tcaattatat cctaagtcta tcgtggttga
      301 ttatactgtg gatggacaat acaaaaatga ttgtcactat ttcggtcaat catcgatcaa
      361 cattgccgat tgggctcaaa acaataatta ttatcctaac ctgatatatg cgattcaaca
      421 gactcttgat ttaatacatt attactctgt agaaacgatt ttcgatttag ctttattgac
      481 acttctgaaa ggtgatttat caatcgatgg tcatgtcgtt tttgatttta aagccccttt
      541 agcaacaagt gcttcaatat gggaaactat taaaactatt gaagattttg atatgatgtc
      601 tcagttttat ttgaataaaa tggcttatat tgatcatcat cctatacctt ttcgcaacct
      661 ttttatcgag gattcagaac aacttaattg gccagatagt tggttgtatt ctaccaagtt
      721 tatgctacct aaatggttat ataaaatagc aaagcaacgt gctgataaca aacaattaca
      781 aaacttcggc ttgtatacga agcaaccaaa cgttttaaaa gatcacatcg tttttatcgg
      841 tgatcatcat caatatatag gtaattcaaa atatttattt acttacttcg ttaaacataa
      901 tccaatgact gcctgttatt ttgttactga cgatcgtcgc ggaccacatt tcatatcacc
      961 taagtctgaa aaagctgacg aattaattaa cagcgcacgc gtagttttag ttgaaaatga
     1021 tatacctgaa acgttacaac caaacggtac tttaattcaa ttacatcaag gcacaccaat
     1081 aatgcaatta tttttagata gtaaagagcc tattaaaaat atagaaacac ctttttatcg
     1141 tgctaaaagg tataatcgct ggttgcaatt cgattatgtt atacattctg ctgatgatat
     1201 tagccacttt tatcaaacgg cttttcctag tcaccaagca aatgtattgg cttatggtaa
     1261 tcccaaacac caatatttac tacaaaagcg taacgaaagt actactcaac aacagtataa
     1321 gaaatcgttt aaaataaatg atcaaaaacc agttttatta tacgcaccta ttggattagt
     1381 gagtgcgcaa caattaccac tatcagatgc tttattcaaa gcctatcatg ttgtggttca
     1441 gggtgttgat gaaacgatgt taccagaaga ggcgcttgtt gcaccaaagt atcttagtgc
     1501 tcaagactta attctaatgt ctgatgtcgt tatcactgat tactcaaata taatatttga
     1561 tgcgatggct atagacaaaa cagtagcatt atacactcca aatcacagtc aatatattga
     1621 gtctcaaggt gttaacgaag atatttggcg tcatctatca aaaatttggt atacagaccg
     1681 acaattatta attaataatt taatttccca ggcaatacca gtgattaaat atccacaaat
     1741 acaacataaa gaacaaccat tagaatctat ttcacaactt atattgtcta aaatgacatc
     1801 taataaatag tgtttaaatc cctcagtggc tgtgaatgag gtttaaaagt actatagaac
     1861 gtaaaccttt ttacttgacg gattgtaata ttaagtgcaa cacctataaa aattcataaa
     1921 aaatgcccat aattggcact ttgttgtaag atgttaataa ccacaaaaa
//
LOCUS       DAPIDM010000224         1905 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000224 DAPIDM010000000
VERSION     DAPIDM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.231"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(107..1015)
                     /locus_tag="RJE42_002676"
     CDS             complement(107..1015)
                     /locus_tag="RJE42_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV5878029.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWNMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVIQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(1118..>1905)
                     /locus_tag="RJE42_002677"
     CDS             complement(1118..>1905)
                     /locus_tag="RJE42_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV5878030.1"
                     /translation="GTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGD
                     VNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLM
                     EDQLKLSDMIIINKIDLVTDDSLEKIDKQLSIICASIPTYKTTYGQVSLEELDLPGKD
                     REISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRDQPNAIYE
                     FQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT          631 a          351 c          270 g          653 t
ORIGIN      
        1 aattacatac ggaataacta taaatttacg ttcaaatctt aattgttcga aacgtttatc
       61 ttcgtttgtc atgatatgct cctttatgat ttcaaagtaa ataagtttac ggaatatctt
      121 gtccgataat agcggtgtaa atgtcaaacc acgactgatc atctaaatta agttgcagtc
      181 cttcgattgc ttgatcaata cgctttaact gacttgttcc aagtatcggc atgatacgat
      241 gcggtatttt tactaaccat gctatcatca cagctgtgtc actcacacca tatttgtcag
      301 ctattgattg aataactttc ataatacgtt gcgctttaat atcttccttg tcgaatattt
      361 taccgcctgc aaaaggactc caagccatga tttgaacatg gttttgatac attgaatcca
      421 ttgttccatc ttgtaaacta tcaacgtgat atggcgataa ttctaattga ttgatgctaa
      481 tatgtagtct ttctttcata atatattgat ttaacaattg gtattgtgaa tgattaaaat
      541 tcgacacccc gaatgacttc aacttacctt gtttaacaag tttagttaat gcatcagcaa
      601 cttgttctgg atccatcaat ggtgaaggac gatgaatgag tagactatct aaataatcta
      661 cattcaaatt gattaatgac tgttcaacag atttcacgat gtgcttacta ctcaaatcat
      721 aacgatgtcc atttgtaaaa tcaaattgct tagaaggcaa aatgatacca catttcgtaa
      781 caatttgaat tttatttctt aattcgggtg ataaatccaa agcattacca aacagtgatt
      841 cacattgata atccccataa atatcagcat gatccatcgt tgtaatccca cgttcaacta
      901 attcatttat aaaataattt aactctttcg cagtcatatt ccattcattt gcacgccaaa
      961 aaccttgtac aagcctagaa aaatgaacat agtgattaat cattatttgt tccatatctc
     1021 atccactcca tccctcaatg tcacaaaata ttcacaacaa aagtattaag aaattaatgt
     1081 gctatttccg ttaaattgaa acaacacatt ttgtgattta cgtaaattgt agcatatcca
     1141 attgattacg tatgtgattt gtatctaaag tttcaccaat aataacaatc gttaatggta
     1201 attgcatgcc aattattcca tagtctggta aaccatatgc atattgaaat tcataaattg
     1261 catttggttg atctctaaat gatacataac ctttcaaacg tagaacagat tccggtaatt
     1321 tcattataaa ttgataaaac aattgacgat caatcggacc tgtaaacgtg taagtcatac
     1381 ttttaatccc atgatgatgg tgatgatgag acgatatctc tctgtcttta ccaggtaagt
     1441 ccaattcttc caacgaaact tgtccatagg ttgttttata agttggaata cttgcacaaa
     1501 ttatacttaa ttgcttatca attttctcaa gactgtcatc agttacaaga tcaattttat
     1561 taataataat catgtcactt agtttcaact gatcttccat cagcgaaact gtattttcgg
     1621 tatattgatg acgttccaaa aatcgagtcg catctaatac accataaata attggctttt
     1681 caaagaaatt aacgatttgc ggatcttgac atgcaactag taattctaaa ggatgcgcaa
     1741 tgcctgtcgc ttctatgatg acatgattaa cgtccccttt taaagcaatg gcttttagtt
     1801 catagacaag ttcttgttta agatcgcaac aaacacaacc attaatcaat gaatggactt
     1861 caatttcatt tgaaatacta ttgctatcaa tatcaaaagt accga
//
LOCUS       DAPIDM010000225         1896 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000225 DAPIDM010000000
VERSION     DAPIDM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.232"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..126
                     /locus_tag="RJE42_002678"
     CDS             <1..126
                     /locus_tag="RJE42_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDV5878031.1"
                     /translation="QGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTENYTG"
     gene            complement(505..1128)
                     /gene="lexA"
                     /locus_tag="RJE42_002679"
     CDS             complement(505..1128)
                     /gene="lexA"
                     /locus_tag="RJE42_002679"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDV5878032.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            1271..1504
                     /locus_tag="RJE42_002680"
     CDS             1271..1504
                     /locus_tag="RJE42_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878033.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNDSEQRDGKVFALIN"
     gene            1640..1879
                     /locus_tag="RJE42_002681"
     CDS             1640..1879
                     /locus_tag="RJE42_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDV5878034.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
BASE COUNT          696 a          274 c          306 g          620 t
ORIGIN      
        1 caaggattaa tgaattctat tggtcacaga aaaaatattt taaatgatac gtttaaaata
       61 ttaggtgtag gtgttgattt taatgatgaa aaacaacctt tttggacaga aaattatact
      121 ggttaatgtt tttggcatga gattaaaggg taatgtttgt ctgtaaggaa gtagataaac
      181 atgatgacaa cagataaacc aaaagatgcg gatatacttg aacgcgtcaa agatatactt
      241 aataaaaaga aagaaaaaaa taataatggc tcgctcctgt aaattattac gggggcgagt
      301 tttaatttgt gtaacatttt gcaggaatct gtaattcaaa atttatatta aagaatgaga
      361 ttatctaaca attcgcaatt tctgtttgaa ctgaatttag attagaataa ataaaacctc
      421 ttgaaaacga aactttttca agaggtccaa aatttataaa aatgagggct taacaattac
      481 gataatatat aacggtttaa aatattacat ttcgcgatac aaaccaatta ctttcccaat
      541 tacagcaaca ttgtcgaggt aaattggctc cattgtacta ttttcaggtt gtaatcgata
      601 acgatttttt tctttataga agcgtttgac agttgcctca tcttcctcag tcatagcaac
      661 aataatgtct ccattttctg ctatggtttg actgcgaaca attactttgt ctccgtctaa
      721 tataccagcc tcaatcatac tgtcgcctac gacgtttaat atgaatatgt cgctattgtg
      781 tgtcgatgtt aagtgttcag gtaatggaaa atattcttca atattttcta ctgcggtaat
      841 aggaacacct gctgtgactt taccaataac tggcacatga atcgtttctt ccatattaat
      901 attatcattt gtttgatcac ttacaatttc tatagcacgt ggtttcgttg gatctcttct
      961 tatatagcct ttttcttcaa gacgtgaaag gtgaccatga acagttgaac tggatgctaa
     1021 gccaactgct tcaccaattt cgcgaacact aggcggataa cctttcattt gaacaacttg
     1081 tttaatatag ttatatattt cgctttgtcg ttttgttaat tctctcatat ataggcactc
     1141 cctaaattaa tttaagtaca gtatatcatt aattcacaac caaaacaaac atttgttcgt
     1201 taaaatattg acacagaaca taagttctga taaattacta atacaaacaa atgttctagg
     1261 agtgaaaatg atgttttaca ataaatataa aaacgtatca acatatatca tcatattttt
     1321 agtttcaagt gcagcctttg caatattctt gttaagtgcg aacattagtg ctcactcgga
     1381 acaagtgtac gaaatgactg accatcaaat taagaacaat acgataaata aagcatacga
     1441 acataaagac cctacaaacg atagcgaaca aagagatggg aaagtgttcg ctttaataaa
     1501 ttgatacatt gtcacaacgt tattttgcct atttttgcga aatagcgttt tttattactt
     1561 ttttgctgat cttaaatttg ttatattttg ttaaagtatt ataatgattg aataaacaaa
     1621 ttgaaggtag gttttttaat tgagtaattc tgatttgaat atcgaaagaa ttaatgagtt
     1681 agctaaaaag aaaaaagaag taggattaac tcaagaagaa gcaaaggagc aaacagcgtt
     1741 aagaaaagct tatcttgaga gttttagaaa agggtttaaa caacaaattg aaaatactaa
     1801 agtaattgat ccagaaggta atgatgtaac acctgaaaaa attaaagaga tacaacaaaa
     1861 aagagataat aaaaattaaa acacaaatct gtaaag
//
LOCUS       DAPIDM010000226         1895 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000226 DAPIDM010000000
VERSION     DAPIDM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.233"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            234..1028
                     /gene="tagH"
                     /locus_tag="RJE42_002682"
     CDS             234..1028
                     /gene="tagH"
                     /locus_tag="RJE42_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDV5878035.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(1088..1852)
                     /locus_tag="RJE42_002683"
     CDS             complement(1088..1852)
                     /locus_tag="RJE42_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDV5878036.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIEL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
BASE COUNT          633 a          313 c          299 g          650 t
ORIGIN      
        1 agttctttaa aatattagtt tattacaaat acattatagt atacaatcat gtaagttgaa
       61 ataagtttag tttttaaata tcattgttat cattgatgat taacattttg tgtcaaaaca
      121 ccaactctga taataacaaa atcttctata cactttacaa caggttttaa aatttaacaa
      181 ctgttgagta gtatattata atctagataa atgtgaataa ggaaggtcta caaatgaacg
      241 tttcggtaaa cattaaaaat gtaacaaaag aatatcgtat ttatcgtaca aataaagaac
      301 gtatgaaaga tgcgctcatt cccaaacata aaaacaaaac atttttcgct ttagatgaca
      361 ttagtttaaa agcatatgaa ggtgacgtca tagggcttgt tggcatcaat ggttccggca
      421 aatcaacgtt gagcaatatc attggcggtt ctttgtcgcc tacagttgga aaagtggatc
      481 gtaatggtga agtcagcgtt atcgcaatta gtgctggctt gagtggacaa cttacaggga
      541 ttgaaaatat cgaatttaaa atgttatgta tgggctttaa gcgtaaagaa attaaagcga
      601 tgacacctaa gattattgaa tttagtgaac ttggtgagtt tatttatcaa ccagttaaaa
      661 agtattcaag tggtatgcgt gcaaaacttg gtttttcaat taatatcaca gttaatccag
      721 atatcttagt cattgacgaa gctttatctg taggtgacca aacttttgca caaaaatgtt
      781 tagataaaat ttacgagttt aaagagcaaa acaaaaccat ctttttcgtt agtcataact
      841 taggccaagt gagacaattt tgtactaaga ttgcttggat tgaaggcgga aagttaaaag
      901 attacggtga acttgatgat gtattaccta aatatgaagc tttcctaaac gattttaaaa
      961 agaaatccaa agccgaacaa aaagaattta gaaacaaact cgatgagtcc cgcttcgtta
     1021 ttaaataaac taaaaaaccg agaatctcca tttaaggatt tcctcggttt tatttttgtc
     1081 atcatgatta tttcgccttt tttatttttc tttttgcttt ggctatttta tacataaata
     1141 taggaatact ctttaatcta ccaatacgtt tccaatctat taatgctcta tatatccatt
     1201 caatgtttaa ttttctaaag atataaggcg ctctcttttt agccccagca aatacttcaa
     1261 gagaaccacc tacgcccatc atcactgtag attcaaattg attttcatgt gtcataatcc
     1321 attcttcttg tttagggaat cccataccta caaatatgta atcaggttta aacagttcaa
     1381 ttcgtttcac taccgtctca tcttctaaat caatataacc gtgatgatgc gcaaacgata
     1441 tgtttggata tctttgttgc aatgcatatt gtgccgcttc tacaacttca ttagttgccc
     1501 ctagcaaaaa tactttttga tgatttacat gagcaatttt caaacattca tccatcaact
     1561 caataccagg tatacgatgc gctagaggtt gctttaaacg atgcgaagct ttgactactc
     1621 ctgtcccatc agcaacaata tagctcgctt gatttattaa ctctaaatac gcttgatgtg
     1681 tcgtcgcgta attcactatt tcagggttgg ctgttactat aaaaagatta ttcgttgatt
     1741 gatttgcaaa aaaggtttta atattttcaa ccatttgcaa cattgttgta ttatcaaaat
     1801 cgacacctaa aatgtcaact ttggctgtat tggatctttc ttcaacagtc ataacaggaa
     1861 tctcctatct tatttcatta ttatgttagt gtaat
//
LOCUS       DAPIDM010000227         1826 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000227 DAPIDM010000000
VERSION     DAPIDM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.235"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            216..1340
                     /locus_tag="RJE42_002684"
     CDS             216..1340
                     /locus_tag="RJE42_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV5878037.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSIDQLERLLKAIRDTFTITGEYSFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIISISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALEGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSRRFKKKFDQSIESVFG
                     QTINNLKEKKLIVEKNDAIALTNRGKVIGNEVFEAFLIND"
     gene            1440..>1826
                     /locus_tag="RJE42_002685"
     CDS             1440..>1826
                     /locus_tag="RJE42_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HDV5878038.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPN"
BASE COUNT          676 a          245 c          331 g          574 t
ORIGIN      
        1 acctctggct aatgtgtaag agccactaca taataaatca ttagtggttc tttattattt
       61 ctatctcact ccctcttaac atttataaat attaaaatga attgatatat tagacaatta
      121 ttgattaaac gttaaagtta aaagtaagaa taattgcaga tagtccaaca ggatatagcc
      181 gattggataa acagtctgag aagcggggca ttaaaatgac ggtacaaagt gcatatatac
      241 atattccatt ttgtgtaaga atatgtacat attgtgattt caataaatat tttatacaga
      301 atcaacctgt agatgagtac ttagatgcac taataacaga aatgtctaca gcaaaatata
      361 ggaacttaaa gactatgtat gtaggtggcg gcacaccaac ggccctttct attgatcagt
      421 tggaaagatt acttaaagca atacgtgata cgtttacaat cacaggtgaa tattcatttg
      481 aagcaaatcc tgatgagtta actaaagaga aagtccaact attagagaaa tatggagtga
      541 acagaatttc aatgggcgtt caaacattca agccggagtt attgtctgtt ttaggtagaa
      601 cgcacaatac tgaagatatt tacacgtcgg tgttaaatgc taaaaacgca ggtattatat
      661 caatcagttt agatttaatg tatcatttac cgaaacagac gattgaagat tttgaacaaa
      721 gtttagatct agctttagat atggatattc aacatatttc gagttacggc ttaatacttg
      781 aacctaaaac ccaattttat aatatgtata gaaaaggttt gctcaaactt ccaaatgagg
      841 atttaggtgc tgacatgtat cagttgctga tgtctaagat agaacaatct cctttccatc
      901 aatacgaaat atctaatttt gcattagaag gccatgaatc agaacataat aaggtttatt
      961 ggtttaatga ggaatattat ggatttggag caggtgcaag tggttatgta gatggtgttc
     1021 gttatacgaa tatcaatcca gtgaatcatt atatcaaagc tataaataaa gaaagtaaag
     1081 caattttagt atcaaataaa ccttctttga ctgagagaat ggaagaagaa atgtttcttg
     1141 ggttgcgttt aaatgaaggt gtgagtagta gaaggttcaa aaagaagttt gaccaatcta
     1201 ttgaaagtgt ctttggtcaa acaataaata atttaaaaga gaagaaatta attgtagaaa
     1261 agaacgatgc gattgcactt acaaatcgag ggaaagtcat tggtaatgag gtttttgaag
     1321 ctttcctaat aaatgattaa aaaaattgaa atatcgagtc tttaacattg acttactttg
     1381 accaatttga taaattataa ttagcacttg agataagtga gtgctaatga ggtgaaaaca
     1441 tgattacaga taggcaattg agtatattaa acgcaattgt tgaggattat gttgattttg
     1501 gacaacccgt tggttctaaa acactaattg agcgacataa cttgaatgtt agtcctgcta
     1561 caattagaaa tgagatgaaa cagcttgaag atttaaacta tatcgaaaag acacatagtt
     1621 cttcagggcg ttcgccatca caattaggtt ttaggtatta tgtcaatcgt ttacttgaac
     1681 aaacatctca tcaaaaaaca aataaattaa gacgattaaa tcaattgtta gttgagaatc
     1741 aatatgatgt atcatcagca ttgacatatt ttgcagatga attatcaaat atatctcaat
     1801 atacaacttt agttgttcat cctaat
//
LOCUS       DAPIDM010000228         1740 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000228 DAPIDM010000000
VERSION     DAPIDM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.236"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..173
                     /locus_tag="RJE42_002686"
     CDS             <1..173
                     /locus_tag="RJE42_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV5878039.1"
                     /translation="EITTDKQVKLVRYKIDESLIPQSLKKDWGSFFMPKFQFEIYGQN
                     DYFIDQITTFTT"
     gene            279..617
                     /locus_tag="RJE42_002687"
     CDS             279..617
                     /locus_tag="RJE42_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878040.1"
                     /translation="MTLEQQLKHYITNLFNLPRDEVWHCESIEEIADDILPNQYVRLG
                     PLSNKTLQTNTYYSDTLHESNIYPFILYYQKQLIAIGYIDENHDMDFLYLHNTIMPLL
                     DQRYLLTGGQ"
     gene            713..1024
                     /locus_tag="RJE42_002688"
     CDS             713..1024
                     /locus_tag="RJE42_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878041.1"
                     /translation="MNRYITRGIANNLPNILQHQLWQLVSEREQEQTKDNTSVDYFHI
                     FQFNKHRNQLYIKHKQERPEYAKIHKANYSKAININKVYIIREDDVDLSYYVMLLPEE
                     Y"
     gene            1040..1546
                     /locus_tag="RJE42_002689"
     CDS             1040..1546
                     /locus_tag="RJE42_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDV5878042.1"
                     /translation="METIKSTLKTEAIFSDDKQHRYLLKKTWNSEKQSITIITMYPHY
                     DGILNIDLTTQLIMNKVSEMDAFGSINFVNLYSNITTPINLKHLENAYDKHTDIQIMK
                     AVKESDEVILAWGAYAKKPGVEARVNEVLEMLKPHKKKVKRLMNPETNEIMHPLNSKA
                     RQKWILKV"
BASE COUNT          685 a          316 c          251 g          488 t
ORIGIN      
        1 ttgaaattac gacagataaa caagtaaaac ttgtaaggta taaaattgac gaaagtctta
       61 tccctcaatc tttgaaaaaa gattgggggt ctttttttat gcccaaattc caatttgaaa
      121 tatatggtca aaatgattat ttcatcgacc aaattaccac ttttaccact tagttattag
      181 tgacaaaagt gagcgaaatg aaataaaaat caaatatata ttatcaaaat gatgtatcac
      241 atgcatacat caatcaaata cattaggagg tcataaccat gacactagaa caacaactca
      301 agcactatat aaccaactta ttcaatctgc caagggacga agtgtggcac tgcgaatcta
      361 tcgaggaaat cgctgatgat atcttaccca atcaatatgt aagacttggc ccactcagta
      421 ataaaacact tcagactaat acctactact ctgacacact tcatgaaagt aatatctatc
      481 ctttcattct ctactatcag aaacaactca tagccatcgg ttatatcgac gaaaatcacg
      541 atatggattt cttataccta cacaacacta tcatgcctct tttggatcaa cgatacttac
      601 taacaggagg acaataaaat gcataaatac atcaaaatta cacaattagt cattacaata
      661 ctaagtgaaa tcatcatttg gatgaaagag tcagaacgaa aggaagtctc ttatgaatag
      721 atatatcacc cggggtatcg ccaacaactt acctaatatc ttacaacacc aattatggca
      781 actcgtatct gagcgagaac aagaacaaac caaagataat acttcagtag attattttca
      841 tatattccag ttcaataagc atcgcaatca attatatatc aaacacaaac aagaacgacc
      901 tgaatatgcg aaaatccata aagctaatta ttcaaaagca atcaatatca ataaggtcta
      961 cattattcga gaagatgatg tagacctttc ttattatgtc atgttattac ctgaagaata
     1021 ctagaggagt gaaaattata tggaaacaat caaaagtaca ttaaaaacag aagccatatt
     1081 cagtgatgac aaacaacatc gctatctact taagaaaaca tggaatagtg aaaaacaatc
     1141 aatcacaatc attacaatgt atccgcatta tgatggcatt ctcaatattg acctaacgac
     1201 ccaactcatc atgaacaaag tttcagaaat ggatgcattt ggttcaatca attttgtgaa
     1261 tctatactct aatattacaa cccctatcaa tctcaaacat ttagaaaatg cgtatgataa
     1321 gcatacagat attcaaatta tgaaggcagt gaaagagtca gatgaagtga tattagcttg
     1381 gggcgcttac gctaaaaagc ccggtgttga agcacgtgtt aatgaagtat tagagatgtt
     1441 gaaaccacat aaaaagaaag taaaacgact catgaatcca gaaaccaatg aaatcatgca
     1501 tccccttaat tcgaaagcac gtcaaaaatg gatattaaaa gtatagcaac taaaatgttt
     1561 attcactatc catgaaaata attgaatttc atatatatct tttaatctac aatatgatta
     1621 aaagatatat tatttggttc tgttgcaaag taaaaaaata tagctaacca ctaatttatc
     1681 atgtcagtgt tcgcttaact tgctagcatg atgctaattt catggcatgg cgaaaatccg
//
LOCUS       DAPIDM010000229         1731 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000229 DAPIDM010000000
VERSION     DAPIDM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.237"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            101..>1731
                     /gene="cna"
                     /locus_tag="RJE42_002690"
     CDS             101..>1731
                     /gene="cna"
                     /locus_tag="RJE42_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HDV5878043.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEAFAARDISSTNVTDLTVS
                     PSKIEDGGKTTVKMTFDDKNGKIQNGDTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKSYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSDYYSGPNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSLNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIKTDANGIANIKALPSGDYILKE
                     IEAPAPYIFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     EDATPEGYTKKENGLVVTNTEKPIETTSISGEKVW"
BASE COUNT          720 a          221 c          332 g          458 t
ORIGIN      
        1 tatgaatttt tgataaattt cattgaataa gaactaaatt agtttataat ttattattag
       61 tatcctgtgg atatgacata gagtataagg aggggttttt atgaacaaaa atgtgttgaa
      121 gtttatggtc tttataatgt tattaaatat catcacacct ttatttaata aaaatgaagc
      181 atttgcagca cgagatattt catcaacgaa tgttacagat ttaactgtat caccgtctaa
      241 gatagaagat ggtggtaaaa cgacagtaaa aatgacgttc gacgataaaa atggaaaaat
      301 acaaaatggt gacacgatta aagtggcatg gccgacaagc ggtacagtaa agatagaggg
      361 ttatagtaaa acagtaccat taactgttaa aggtgaacag gtgggtcaag cagttattac
      421 accagacggt gcaacaatta cattcaatga taaagtagaa aaattaagtg atgtttcggg
      481 atttgcagaa tttgaagtac aaggaagaaa tttaacgcaa acaaatactt cagatgacaa
      541 agtagctacg ataacatctg ggaataaatc aacgaatgtt acggttcata aaagtgaagc
      601 gggaacaagt agtgttttct attataaaac gggagatatg ctaccagaag atacgacaca
      661 tgtacgatgg tttttaaata ttaacaatga aaaaagttat gtatcgaaag atattactat
      721 aaaggatcag attcaaggtg gacagcagtt agatttaagc acattaaaca ttaatgtgac
      781 aggtacacat agcgattatt atagtggacc aaatgcaatt actgattttg aaaaagcctt
      841 tccaggttct aaaataactg ttgataatac gaagaacaca attgatgtaa caattccaca
      901 aggctatggg tcacttaata gtttttcaat taactacaaa accaaaatta cgaatgaaca
      961 gcaaaaagag tttgttaata attcacaagc ttggtatcaa gagcatggta aggaagaagt
     1021 gaacggaaaa tcatttaatc atactgtgca caatattaat gctaatgccg gtattgaagg
     1081 tactgtaaaa ggtgaattaa aagttttaaa acaggataaa gataccaaag ctcctatagc
     1141 taatgtaaaa tttaaacttt ctaaaaaaga tggatcagtt gtaaaggaca atcaaaaaga
     1201 aattgagatt aaaacagatg caaacggtat tgctaatatt aaagcgttgc ctagcggaga
     1261 ctatatttta aaagaaatag aggcgccagc accgtatata tttgataagg ataaagaata
     1321 tccgtttact atgaaagata cagataatca gggatatttt acgactattg aaaatgcaaa
     1381 agagatagaa aaaacaaaag atgtttctgc tcaaaaggtt tgggaaggca ctcaaaaagt
     1441 gaaaccaacg atttatttca agttgtacaa acaagatgac aatcaaaaca caacaccagt
     1501 agacaaagca gagattaaaa aattagaaga tggaacgaca aaagtgacat ggtctaatct
     1561 tccagaaaat gacaaaaatg gcaagactat taaatatttg gttaaagaag taaatgctca
     1621 aggtgaagat gcaacaccag aaggatatac taaaaaagaa aatggtttag tggttactaa
     1681 tactgaaaaa ccaatcgaaa caacatcaat tagtggtgaa aaagtatggg a
//
LOCUS       DAPIDM010000230         1681 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000230 DAPIDM010000000
VERSION     DAPIDM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.238"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..464)
                     /locus_tag="RJE42_002691"
     CDS             complement(<1..464)
                     /locus_tag="RJE42_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDV5878044.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTRNLNDTVASMIIEFVEWI
                     NTLEFFKNWKKQPGKPLDTQLDEIADYLAFSLQLTLTIVDEEDLEETTEVMVDLIENE
                     VTLPKLHSVYFVHVMHTLTEQFVKGIDNSIVQVLIMPFLYANTYYSIDQLIDA"
     gene            complement(457..705)
                     /locus_tag="RJE42_002692"
     CDS             complement(457..705)
                     /locus_tag="RJE42_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDV5878045.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIYLDEAVGIMTGQVVYKYEEEQENN"
     gene            complement(698..1063)
                     /locus_tag="RJE42_002693"
     CDS             complement(698..1063)
                     /locus_tag="RJE42_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV5878046.1"
                     /translation="MNYETGFQLGVMDARLKKMRKQRDEYKKQRDELIVDIAKLRERN
                     EELENMWRTVKNELLGRYEFYRFRLNELQIESRANKAVAINMGAKINASAILYRMDKL
                     DGTNEFYEFLGQMEDDTNE"
     gene            complement(1060..1497)
                     /locus_tag="RJE42_002694"
     CDS             complement(1060..1497)
                     /locus_tag="RJE42_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878047.1"
                     /translation="MMPKYRVWDTETKKMCEVVALDLHNSEVSYSTKENEYGKVIKEF
                     IKTEKMADVELMQSIGINLWGRELYEGDILKVVSTKLWGIERDKTYIYLDATGVVTRN
                     AIGTIIGDVHLLRVFEAEEVREMPTIEYLGNKFENPELLEVPE"
BASE COUNT          447 a          374 c          231 g          629 t
ORIGIN      
        1 gcgtcaatga gttggtcgat agaatagtaa gtattggcgt acaaaaacgg cattattaaa
       61 acttgtacaa tgctattatc aatacctttt acaaattgtt ctgttagtgt atgcattaca
      121 tgaacaaaat aaactgaatg tagtttaggt aaagtaactt cattttcaat caaatcaacc
      181 ataacctcag tagtttcttc caaatcttct tcatcaacaa tagtcaaagt taattgcaaa
      241 ctgaaagcta agtaatcagc aatctcatct aattgtgtat ctagtggctt acctggttgt
      301 ttcttccaat ttttaaaaaa ctcaagtgtg ttaatccact ctacaaattc aataatcata
      361 ctagctactg tgtcatttaa atttctagtt ggtattctat cgtcgaactc cttttgtatt
      421 tgtaataact cttgtaactg atcaattgtt aatgtgttag ttattttcct gttcctcctc
      481 atatttatag acaacttgac ccgtcataat ccctactgct tcatcaagat aaatatcttc
      541 tttgagtgca tcttgcatag cattaggtaa accctcaagt atttcatcaa acgcttgtgc
      601 tttcttatac acgtcctcaa tctcttttag taatccctct gtgtcattgc cgttatacgc
      661 actagcactt ataacggact gttctatttg ttcacggtta ttcattagtg tcatcctcca
      721 tttgtcctaa aaattcgtag aactcatttg ttccgtctaa tttgtccatt cggtacaata
      781 tagcacttgc gttgatttta gctcccatgt ttatagctac tgccttgttc gctctactct
      841 caatctgtag ttcgttaagt ctaaaacggt aaaattcgta tcttccaagc aattcatttt
      901 tgactgtgcg ccacatgttc tccagctctt cgttacgctc tcttaactta gctatatcca
      961 cgataagctc atcgcgttgc ttcttgtact catcacgttg ttttctcatc ttcttcaacc
     1021 tagcgtccat tacacctagt tggaaccctg tttcatagtt cattctggca cctccagtaa
     1081 ctccggattt tcaaacttat tgcccaagta ttcaatagtt ggcatttcac gaacttcttc
     1141 agcctcaaaa actctcaata gatgtacgtc gccaattata gtgccaatag cgtttcgagt
     1201 gactacgcct gtagcatcta aataaatgta tgttttatcc cgttcgatgc cccacagttt
     1261 cgttgatacg acttttaata tatcgccctc gtataattct cttccccaca gatttatacc
     1321 aattgactgc ataagttcta catctgccat tttctcagtc tttataaact cctttataac
     1381 cttgccgtat tcattttctt tagttgaata actaacttcg ctattgtgaa gatctaacgc
     1441 cacaacctca cacatctttt ttgtttcggt gtcccatact cgatatttcg gcatcattct
     1501 actacctcca ctttttcgac ctctatgctt gcagttttga atgggagttt tttacgagtc
     1561 agttttaatg ccatattctt agcttcttcc tcatttatac tttgcacaaa ataatgcttt
     1621 tttattttgt aatcacattt agatgctaag aacttgatac aaagacttac tttataggtt
     1681 t
//
LOCUS       DAPIDM010000231         1672 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000231 DAPIDM010000000
VERSION     DAPIDM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.239"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..818
                     /locus_tag="RJE42_002695"
     CDS             <1..818
                     /locus_tag="RJE42_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV5878048.1"
                     /translation="TYPKVENMQNNDIKKIFTIHTNHFMSPYSYGAEIKPEFRNMLLN
                     LKELDTLVVLTKEQKDDIIKQFGDYNNIKVIPNAISFEENLTQNIREKNSIIVLQRFV
                     AMKNITHIISAINIVRKKVKDVKLHIYGTGTQKENYTKLIKKLKLQDNVFIHDYAFDI
                     RALYTKASLSVLTSDYEGLPMSLLEAMSYGVPVISYPINYGPKSIIQNNINGIITKKK
                     DNINELAKKIIHVLKKETLISQFSENARTTIKTNFSYEAVSKKWIELIDEKSQ"
     gene            complement(1051..>1672)
                     /locus_tag="RJE42_002696"
     CDS             complement(1051..>1672)
                     /locus_tag="RJE42_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDV5878049.1"
                     /translation="TFKPLHIFSDLSSKMFTWNNGEYIESETYKKKVLSEPFLFEDIE
                     DILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHSNDRRILEALVRYANDQNYTA
                     RFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDSGNDEAYL
                     SYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
BASE COUNT          636 a          263 c          216 g          557 t
ORIGIN      
        1 gtacttatcc taaagtagag aacatgcaga ataatgatat caaaaaaata tttactatac
       61 atacgaatca tttcatgtcc ccttactcct atggcgctga gattaaacca gagttcagaa
      121 acatgttact aaaccttaaa gagttagata ctttagttgt attaactaaa gaacaaaaag
      181 atgacattat aaaacaattt ggtgattaca ataatataaa ggttattcct aatgctatat
      241 cttttgaaga aaatttaaca caaaatattc gagaaaaaaa ttctattatt gtattacaaa
      301 gatttgtagc tatgaaaaat atcacacata tcatttccgc tataaatatt gtaagaaaaa
      361 aagtaaagga tgttaagtta cacatatacg gtaccggtac gcaaaaagaa aattacacaa
      421 aacttattaa aaaacttaaa ttgcaagata atgtgttcat ccatgattac gcatttgaca
      481 tacgtgctct ttatacaaaa gcctctctaa gtgtactgac atcagattat gaaggtctcc
      541 caatgagttt actagaggcc atgagttacg gtgttccagt tatttcatat cctattaatt
      601 atggtcctaa atctataatc caaaataata taaatggaat aataacaaaa aagaaggata
      661 atattaatga attggcaaaa aaaataattc atgtattaaa aaaagaaaca ttgatatctc
      721 agttttctga aaatgccaga acaacaataa agacaaactt ttcttacgaa gcagtatcca
      781 aaaaatggat tgaattaatc gatgaaaaaa gccaatagcg atcaaagccc tctatatctt
      841 caatagtatc aatattaaat gtttatttaa tttacaaagt gttcaaatat attgattaaa
      901 ctatgaatcg tctacactta gttagacaaa tcaacataca aataaatttt ttaaaactaa
      961 agtgtagtcg attcaccata ttatctaatc attcctcaaa ttttaaatat taatatttgc
     1021 acttaaaaag tatacatatc actcaaacaa tcaataatca tacttacctt caacaaattc
     1081 tttaacatgc agtgttatcc cttgataata tggatatttt gttaatctaa atttctgctt
     1141 taatgcttca tctctactat tcgacatcaa ataggcatgt tcaagatagc ttaaatatgc
     1201 ttcatcattg ccgctatctc caaaacctaa tattgacttt actggtatat tgtatttttt
     1261 catcagaaat tgagtagcat ataattttcc agcatttgac ggtgtgaagt caatatcata
     1321 cgcattttca ggatcaccag ctaatgggtt gcttcgacta aatctcgctg tatagttttg
     1381 gtcatttgcg tatctaacaa gtgcttctag gatacgccta tcattgctgt gattacctgt
     1441 tgaatgaaaa taaaaattat aatgtgtttc attaccttca aatgctcttt gtggaataaa
     1501 ttcgacatta tattgcgcag aaatatgacg taatatatct tcaatatctt caaataaaaa
     1561 tggttcgctc aagactttct ttttatatgt ttctgattca atatattcgc cattattcca
     1621 agtgaacatt ttagaactca aatcagaaaa gatatgtaat ggtttaaacg tc
//
LOCUS       DAPIDM010000232         1663 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000232 DAPIDM010000000
VERSION     DAPIDM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.240"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..341
                     /locus_tag="RJE42_002697"
     CDS             <1..341
                     /locus_tag="RJE42_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878050.1"
                     /translation="KFKAWDKDKKVMSIIDEIDFNSGYILISTGYKSFNEVKLLQYTG
                     FKDVHGVEIYEGDIVQDCYSREVSFIEFKEGAFYITFSNVTELLSENDDIIEIVGNIF
                     ENEMLLEVMR"
     gene            338..646
                     /locus_tag="RJE42_002698"
     CDS             338..646
                     /locus_tag="RJE42_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878051.1"
                     /translation="MTFTLSDEQYKNLCTNSNKLLDKLHKALKDREEYKKQRDELIGD
                     IAKLRDCNKELEKKASAWDRYCKSVEKDLINEFGNDDERVKFGMELNNKIFMEDDTNE
                     "
     gene            639..890
                     /locus_tag="RJE42_002699"
     CDS             639..890
                     /locus_tag="RJE42_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDV5878052.1"
                     /translation="MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQENEY"
     gene            880..1053
                     /locus_tag="RJE42_002700"
     CDS             880..1053
                     /locus_tag="RJE42_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878053.1"
                     /translation="MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIID
                     FITKPIYLIKGDE"
     gene            1054..1215
                     /locus_tag="RJE42_002701"
     CDS             1054..1215
                     /locus_tag="RJE42_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878054.1"
                     /translation="MLEIIDQRDALLQEKCLNDDWWYELDYWLNKRKSENEQIDIDRV
                     LKFIEELKR"
     gene            1230..>1663
                     /locus_tag="RJE42_002702"
     CDS             1230..>1663
                     /locus_tag="RJE42_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDV5878055.1"
                     /translation="MNNLTVDQLQELLQIQKEFDDRIPTLNLGDSKIAYVVEFFEWFN
                     TLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQQEVTNEKLEYGLSTLRKDGYLY
                     NESQSVWDFMSDVSNVGLEPLSAVIIPLDIAYNLYSIDQLIDA"
BASE COUNT          634 a          188 c          368 g          473 t
ORIGIN      
        1 tgaaatttaa agcttgggat aaagataaaa aagttatgag tattattgac gaaatcgatt
       61 ttaatagtgg gtacattttg atttcaacag gttataaaag tttcaatgaa gtaaaactat
      121 tacaatacac aggatttaaa gatgtgcacg gtgtggagat ttatgaaggg gatattgttc
      181 aagattgtta ttcgagagaa gtaagtttta tcgagtttaa agaaggagcc ttttatataa
      241 cttttagcaa tgtaactgaa ttactaagtg aaaatgacga tattattgaa attgttggaa
      301 atatttttga aaatgagatg ctattggagg ttatgagatg acgttcacct tatcagatga
      361 acaatataaa aatctttgta ctaactctaa caagttatta gataaacttc acaaagcatt
      421 aaaagatcgt gaagagtaca agaagcaacg agatgagctt attggggata tagcgaagtt
      481 acgagattgt aacaaagaac tggagaagaa agcaagcgca tgggataggt attgcaagag
      541 cgttgaaaaa gatttaataa acgaattcgg taacgatgat gaaagagtta aattcggaat
      601 ggaattaaac aataaaattt ttatggagga tgacacaaat gaataatcgc gaaaaaatcg
      661 aacagtccgt tattagtgct agtgcgtata acggtaatga cacagagggg ttgctaaaag
      721 agattgagga cgtgtataag aaagcacaag cgtttgatga aatacttgag ggaatgacaa
      781 atgctattca acattcagtt aaagaaggta ttgaacttga tgaagcagta gggattatga
      841 cggggcaagt ggtctataaa tatgaggagg agcaggaaaa tgagtattag tgtaggagat
      901 aaagtatata accatgaaac aaacgaaagt ctagagattg tgcaattggt cggagatatt
      961 agagatacac attataaact gtctgatgat tcagttatta gcattataga ttttattact
     1021 aaaccaattt atctaattaa gggggacgag taaatgcttg aaatcatcga ccaacgtgat
     1081 gcattgctac aagaaaagtg tttaaacgac gactggtggt acgagttaga ttattggttg
     1141 aataaacgca agtcagaaaa tgaacagatt gatattgata gagtgcttaa atttattgag
     1201 gaattaaaac gataggagat aacgaataaa tgaataattt aacagtagat caattacaag
     1261 agttattaca aatacaaaag gagttcgacg atagaatacc gacgctgaac ttaggagata
     1321 gcaagattgc atatgtagtt gaattctttg aatggtttaa tacattggaa acgtttaaga
     1381 actggaagaa gaaaccaggt aagccgttag acgtacagtt agatgaatta gctgacatgt
     1441 tggcgtttgg attgagtatt gcaaatcaac aagaggtaac gaacgaaaaa ttagaatatg
     1501 gattaagcac tcttagaaaa gatgggtatc tttacaatga atctcaatcc gtttgggact
     1561 ttatgtcaga tgtatcaaac gttggtttag aacctttaag tgcagttatt ataccactag
     1621 atattgctta caacttatat tctatcgacc aactcattga cgc
//
LOCUS       DAPIDM010000233         1653 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000233 DAPIDM010000000
VERSION     DAPIDM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.241"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            195..389
                     /locus_tag="RJE42_002703"
     CDS             195..389
                     /locus_tag="RJE42_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV5878056.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(623..1474)
                     /locus_tag="RJE42_002704"
     CDS             complement(623..1474)
                     /locus_tag="RJE42_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDV5878057.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEFVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          663 a          249 c          249 g          492 t
ORIGIN      
        1 accaatacat aataaagcat aagtgattct ttatcatttc tctcccactc cctcaagtta
       61 atgatgaatc tatttcgcta gttataaaat gacgtcaaat caatatcaaa aattatttaa
      121 gtaaaatgtt tagataattt ttcagtgggt aagtattata tataacattt aattattccg
      181 aggaggcatt tattatggca gacgaaagta aatttgaaca agcaaaaggt aatgttaaag
      241 aaacagtagg taatgttact gataataaaa atttagaaaa cgaaggtaaa gaagataaag
      301 cttctggtaa agcgaaagaa ttcgttgaaa atgcaaaaga aaaagcaact gattttattg
      361 ataaagtaaa aggtaacaaa ggcgagtaat tttacttttt attcctaata cactgcacat
      421 gccacttagt cggtataacc atcggctagg tggttttttg tattaaaaaa gtggacacca
      481 aaatttatta ataattattt taatgttaga aaaaaactaa ataaaaactc gctaatgata
      541 tccaataata tgtatacaaa acgagacata tattgcatat gattaacgag atactgaata
      601 tattttatca cccctaaaat gattattcat tttcagcggt aattcgacct aaagtcaaac
      661 ttacaataaa accgatgata aatactacta atgaaacgaa ccacatcacg atattagttg
      721 gtaaacctgg aaatactgca aagagggagc caacaacaaa accaatgatt aatgcaaaag
      781 tcattagttt atgatgtgtt aggaaatact ggataatttt acttgaaata atgaatccag
      841 caagcacgcc aaatccgact gcaagtaata taggaagacc ggcaaagtta agtttaacaa
      901 attcagatat tgctagcatg accgtaccat agacgccaaa tactaataac ataaatgacc
      961 ctgaaatacc tgggagtaac atagcactag atgcacacat acctgcaata aagtatttaa
     1021 taataagacc agttgataga gtaagtgttt ctccagcatg tttatcgcca ttattcatta
     1081 atgtaataac aattaagata gcgataccag ctataaccat catgtaatgt ttagttgtaa
     1141 atgacgtttt atagttagaa attttcaata aatatggaac gataccaatg attaatccac
     1201 caaagaaaaa catagttgga atatggtgtt ggcttaataa ataattaaaa agattactta
     1261 gtgatcccat tgccagtaac attccaatta taatagggat taaaaatgta aaacttggcc
     1321 aaaaacgtcg tgagaatatg ccactaattg aagcgataaa ttgattgtat atacctaaca
     1381 ataatgcaat agtcccaccg ctaacaccag gtaccaagtc actcgttccc atagcaaaac
     1441 cttttagaat attaatccat ttaaattgtt gcatgaataa ctcctttcaa acgattggaa
     1501 taaaatcata aatagcatca taccatatta caaatgtcct agtgaaatga taacatattt
     1561 taaattcata aaatccattg agaaattatg tgcacttatt atcatttata tttttaaaga
     1621 gagcggatta gagttgtact ttagagttat taa
//
LOCUS       DAPIDM010000234         1649 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000234 DAPIDM010000000
VERSION     DAPIDM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.242"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            227..1591
                     /gene="radA"
                     /locus_tag="RJE42_002705"
     CDS             227..1591
                     /gene="radA"
                     /locus_tag="RJE42_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV5878058.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
BASE COUNT          590 a          230 c          350 g          479 t
ORIGIN      
        1 agagaaaaga gtttactaag aaattattaa gattttatct ttgaaagata ccgactatca
       61 acatgatgta agtttatttt ataatattca taaaaaataa atatgataaa acagtttgcg
      121 ttagtagtca tgttaaaata caaacaaatg taactcacat ttaatttgtc ataatgggaa
      181 tgtgcgttta aaatagattt gttcaaagga aagtggaggt gcaattttgg ccaagaaaaa
      241 agtgattttt gaatgtatgg cttgtggtta tcaatctcct aaatggatgg gaaaatgtcc
      301 taattgtggc gcttggaatc aaatggagga aattgttgaa aaagcagcca atcctaaaca
      361 tggagtgaaa accaaggaat tagcaggtaa agtacaaaaa ttaaatagta ttaaacatga
      421 aacaacgcca agagtgttaa cagattcagc agaattcaac cgtgtattag gtggaggtat
      481 tgtgagcgga tcgttagtac ttattggtgg ggatccaggt attggtaagt caacgttact
      541 tttacaaatt tgtgcatcgt tatctcaaaa gaaaaaagta ctatatatta ctggagaaga
      601 atcgcttagt cagactaaat tacgtgcaga gcgattagat gaagattcaa gtgaattgca
      661 agtgttagct gaaacagatc ttgaagttat ttatcaaaca gtaaaagaag aacaacctga
      721 tttactagta gtggattcga ttcaaacaat atatcatcct gaaatcagct ctgcaccagg
      781 ttctgtttca caagttcgtg aaagcacaca aagtttaatg aatattgcta aacaaatgaa
      841 cattgcaact tttatagtgg gtcatgtaac gaaagaaggt caaattgctg gcccaagatt
      901 gctagaacac atggttgata ctgtgcttta ttttgaaggc gatgaacacc acgcatatcg
      961 aattttgcga gctgttaaaa accgttttgg ttcaacgaat gaaatgggaa tcttcgaaat
     1021 gaagcaaagt ggattaaaag gtgtaaataa tccatctgaa atgtttttag aagaacgttc
     1081 aacaaatgtt ccaggttcaa caattgttgc aaccatggag ggaaccagac cacttttaat
     1141 agaagttcaa gcgctggtaa ctccaacgac ttttaacaat ccgagacgaa tggcaacagg
     1201 gattgatcat aatcgattaa gtttgttgat ggctgttttg gaaaagaaag aaaattatct
     1261 attacaacaa caagatgctt atatcaaagt agctggcggt gtaaagttaa cggagccagc
     1321 agttgatcta agtgtaattg tagcaactgc gtctagcttt aaagataaag ctgtcgacgg
     1381 attagattgc tatattggag aagttggttt aacgggtgag gtacgtcgtg tatctcggat
     1441 agaacaacgc gtgcaagagg ctgcaaaact aggtttcaaa cgtgtaatta ttcctaaaaa
     1501 taatataggc ggatggacat atcctgaagg tatacaagta ataggtgtaa ctactgtaca
     1561 tgaagcattg tcgtttgctc ttcattcata aaacatcaag aaaggaggac attgtgtgaa
     1621 tatcgttaaa ctaatggtta ttattattt
//
LOCUS       DAPIDM010000235         1558 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000235 DAPIDM010000000
VERSION     DAPIDM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.243"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(1..95)
                     /locus_tag="RJE42_002706"
                     /pseudo
     CDS             complement(1..95)
                     /locus_tag="RJE42_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            145..1558
                     /locus_tag="RJE42_002707"
                     /pseudo
     CDS             145..1558
                     /locus_tag="RJE42_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078365.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          566 a          232 c          295 g          465 t
ORIGIN      
        1 gcacgattta ataattgaac aatatggaat cgatcgataa ctattttagc gttcggaaac
       61 atctctttaa tcaatgtcat aaagccttca tgcattgagg cgctcccacc tattttgagt
      121 gaagatgatg tgatggatcg atttatggtg gcatcacgaa taacaaatca agataagcag
      181 agtgcgacaa acattcgcta tcatgtgctt aaacgtataa aaaaatttat tcagccatta
      241 cctattcatt tcgaagtaaa acgtcgatta tctacgttga ttcgtagagg ttacttagcc
      301 agaaaccctt tggataaaac atttttggaa cgtatttgag ttttacacca attacgagaa
      361 gatgaagaag atgctcataa atatatagat gaacgcttaa attacattcg ttcgacagca
      421 gatagcttat ctattattgg catttcagat atcggaaaaa caacagcaat tgaacgctta
      481 ttgttaatgt atccacaagt aattaagcat tagaagtatg aagaacaacc atttaatcgt
      541 acacaaattg tctggctgaa aattgattgc ccgtatgacg gcagcctttc cacactatgt
      601 aaaatcttct ttaaagcgat tgatgaatta ttaggaacgc gttatttaga gaaatttggc
      661 tacttaaacc gtgtaacatc aactatgttg ttacatagga cttcattagc taatatgtat
      721 ggtgttggcg tacttgtaat tgatgagatt caacatttat tacatgcaaa aaatgacaaa
      781 gaagatatgc ttaatttctt tgtaaccttg tctaatacgg ttggtgttcc aacagttttg
      841 attggtacat ctaaggctca acaattattt aaaggaaact tccgtcaggc tcgtcgtgca
      901 gccagtgaag gttcgattat ttgggatcgc atgaatgagg atagtgaaga atgggagttc
      961 tttttagaaa cattatggga attacaatgt cttaaaactt actcagagct tacacaggac
     1021 gttaaaaagg ctttctataa tgactgtcag gggattactt cagtcgctgt taatctattt
     1081 atattggctc aagaaagagc actatatgat gaatcgaacg aagatgaaaa attgacgata
     1141 agagttttaa agaaaacggc aaaagaagat atgaaaatca ttcagccaac gttaaatgct
     1201 attcgaaaaa atgattttag agcaatgtat aagtatgaag atattatgat taatttagat
     1261 gaattaatgc aaagtcataa gcaaaatact gagtatgaag gtccaattaa agaggccatg
     1321 aaagagcgaa aaaatacttt aacatacaag cgccaagagt tgattgaagg tttgagtgaa
     1381 gaagttgcag caatcggcat ttttgatgct ttaactccaa gtcacattaa agaaactaca
     1441 aaaaaattag tagaaaattc atcgatagaa acggattata acctactgaa aacacaactt
     1501 attcctaatt taattgagtt gaataagaag cttaatgaga aaaaagtgaa ggtagtac
//
LOCUS       DAPIDM010000236         1484 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000236 DAPIDM010000000
VERSION     DAPIDM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.244"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            12..1310
                     /locus_tag="RJE42_002708"
     CDS             12..1310
                     /locus_tag="RJE42_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDV5878059.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVIDFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANQ
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
BASE COUNT          546 a          219 c          297 g          422 t
ORIGIN      
        1 agaggtaaag aatgacaaaa catgaacaaa ttttagatta tattgaatcg ttatctatag
       61 gctctaaaat ttcggtgcgt aaaatcgcta aatttttaaa cgtatctgaa gggacagcat
      121 atcgtgcaat taaagatgct gataaaatgg gcatggtcgc aacgatagat cgtgttggta
      181 cggttcgaat cgaaaaacgt aatcgtaatg aaattgaaca tttaactttt aatgaaattg
      241 tcaatattat tgatggccaa gtgttaggtg gcaacaaagg tatcactaag atggtatcta
      301 aatttgcaat tggagcaatg gaactcaaag acattttaaa gtatattggt ccaaagacat
      361 tattgattgt cggaaatcgc gaagatgtac agattgaagc tttaaaaaga ggaacggcta
      421 tattaattac tggtggattt aagccatcta ataaagttat tgattttgcg aatgaacatg
      481 atttacctgt tctatcatca agttatgata cattcctagt tgcaaatatt atcaacaaag
      541 cactttttaa tcagaaaata cgaaaagata tcttaatagt tcaagatata atgacgccat
      601 tagatgattt atctgtgctg tttgatacga tgaaaatagc agattataag cgtatggcga
      661 atcaaactgg ccatacaaga tttccggtag tcaatgagtc ttataaatta gtaggtattg
      721 taacaagtcg agagatgatt aatacgaagg acgacgacga aatagataaa gtgatgacgc
      781 gaaatcctat ttatgtaaat gctatgagta cagtagcaag ttgtgcgcat atgatgattt
      841 gggaaggtat tgaattgatt cctgtcgttt cgagtaataa aaagacagtg ggtgtgatta
      901 atagacaaga tgttttgaag tctatgcaac ttttaggaag acaacctcaa atgggtgaaa
      961 caattaacga tcaaatcgcc aaatatataa caatgaatca agatggaatt acagtagaag
     1021 tttcaccact gttaatcaat cactacggaa cagtaagtaa agcagcattt gtaagtatta
     1081 tagaagaaac aatacaatat gaaatgcgta aatttaaaaa aggtaatgtt atgatagaga
     1141 acttaaatat cgtgtatatt aaaacagtac ctattgaatc acatataaca gtgcgctttg
     1201 gcatactaga cgtcggaaga aacttcgcca aaatagaagt gaacatgcat agtcaaaacg
     1261 acaaagtagc gagcgctctc gtgatatgcc aaatgtttga cgaagtatag gaacttagca
     1321 gagaaaatag gcataagata gatttcacac aaatgttgga gcccaacttg cattgtctgt
     1381 agaaattgga aatccaattt ctctatgttg gggccccagc ccaacttgca cattaaagta
     1441 agctgacatt acgtcacctt ctatgttggg cccccacccc aact
//
LOCUS       DAPIDM010000237         1483 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000237 DAPIDM010000000
VERSION     DAPIDM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.245"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            45..>1483
                     /locus_tag="RJE42_002709"
     CDS             45..>1483
                     /locus_tag="RJE42_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV5878060.1"
                     /translation="MKGKIALYSRVSTSEQSEHGYSIHEQEQVLIKEVVKNFPGYDYE
                     TYTDSGISGKNIEGRPAMKRLLQDVKDNKIEMVLSWKLNRISRSMRDVFNIIHEFKEH
                     DVGYKSISENIDTSNASGEVLVTMFGLIGSIERQTLISNVKLSMNAKARSGEAITGRV
                     LGYKLSLNPLTQKNDLVIDENEANIVREIFDLYLNHNKGLKAITTVLNQKGYRTINQK
                     PFSVYGVKYILNNPVYKGYVRFNNHQNWAVQRRSGKSDKNDVILVKGKHEAIISEEVF
                     DKVHEKLASKSFKPGRPIGGDFYLRGLIKCPECGNNMVCRRTYYKTKKSKERTIKRYY
                     ICSLFNRSGSSACHSNSINAEVVERVINVHLNRILSQPDIIKQIASNVIEELKQKHSN
                     QTEIKYDIDSLEKQKAKLKTQQERLLELFLDDQMDSEMLKAKQSQMNQQLEVLDQQIK
                     EAQQANQSQDEIPNFDKLKGRLILMITRF"
BASE COUNT          554 a          221 c          293 g          415 t
ORIGIN      
        1 accgatagtc tgtagcaata atctaataaa aggagcggta tgatatgaag ggtaaaattg
       61 cactttattc acgcgttagt acgtcagagc agtcggagca tgggtactca atccatgagc
      121 aggaacaagt actcatcaaa gaagttgtga aaaatttccc aggttatgac tatgagacat
      181 atactgactc aggcatttca ggtaaaaata ttgaaggtcg tccggcaatg aaacgtctat
      241 tacaagatgt taaggataat aaaatcgaaa tggtgttaag ttggaaattg aatcgtatct
      301 cacgatcaat gagagacgtg tttaatatta ttcatgaatt caaagaacat gatgtagggt
      361 ataaatcgat ttctgagaat attgatacat ccaatgcttc tggagaagta ctcgttacaa
      421 tgtttgggtt aataggatct atagaacgcc agactttgat ttcgaatgtg aaactttcta
      481 tgaatgctaa ggcaaggagc ggagaggcaa tcaccggtcg tgttttaggc tacaaattat
      541 cacttaatcc acttacacag aaaaatgatt tggttatcga tgaaaatgaa gctaatattg
      601 tacgtgaaat tttcgattta tatttgaatc ataataaagg cctcaaagcc attacaaccg
      661 tacttaatca aaaggggtat cgtactatta atcaaaagcc attttcagtg tatggtgtta
      721 aatacatttt gaataatcca gtctataaag gctatgtcag attcaataac catcaaaact
      781 gggctgtaca gcgaagaagt ggcaaaagtg ataaaaatga tgtgatattg gtcaaaggta
      841 aacatgaagc cattataagt gaagaggtat ttgataaagt tcatgaaaaa ttagcttcta
      901 aaagttttaa accgggcaga cctattggtg gagatttcta cttacgtggc cttattaaat
      961 gcccagaatg cggaaataat atggtatgtc gacggacgta ttataaaacg aaaaagtcca
     1021 aagaacggac aatcaagcgt tattacattt gttccttatt caatcgttca gggagttctg
     1081 catgtcacag taattccatc aatgctgaag tcgtcgagcg tgtaattaat gttcatttga
     1141 atcgtattct gtctcaacca gatattatca agcagattgc gtcaaatgtg atagaagaac
     1201 tgaaacaaaa gcatagtaac caaacagaaa ttaaatatga cattgatagt ttagaaaaac
     1261 aaaaagctaa gcttaaaaca caacaagaac gattgttaga attgttctta gatgatcaga
     1321 tggatagcga aatgttaaaa gctaaacaaa gtcaaatgaa tcaacagtta gaagtattag
     1381 atcaacaaat taaagaagcg caacaagcaa atcaatcaca ggatgaaata cctaattttg
     1441 ataaattaaa aggacgactc attttgatga taacacgatt cag
//
LOCUS       DAPIDM010000238         1476 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000238 DAPIDM010000000
VERSION     DAPIDM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.246"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            105..1031
                     /gene="hemC"
                     /locus_tag="RJE42_002710"
     CDS             105..1031
                     /gene="hemC"
                     /locus_tag="RJE42_002710"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV5878061.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDDELLSLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            1053..>1476
                     /locus_tag="RJE42_002711"
     CDS             1053..>1476
                     /locus_tag="RJE42_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV5878062.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLSAALSKDNEVVKIDLYTSVP"
BASE COUNT          541 a          202 c          274 g          459 t
ORIGIN      
        1 aacattattt tattttgctt aaatatgatt aatttatttt ttgcaacaca ttttgtgaat
       61 tgagaaaata atctagagca atccgttcct aggaggaatt gaacatgcgt aaattagtcg
      121 ttggctccag aagaagtaaa ttagctttaa cacaaagcca gcaatttatt gataaattaa
      181 aagctgtaga gccaaatcta gaaattgaaa ttaaagaaat tgtcacgaaa ggcgatcgta
      241 tagtagataa acaattgtct aaagtcggag gcaaaggctt atttgttaaa gaaatacaac
      301 atgaactttt tgaaaaaaat atcgatatgg caatacactc gcttaaagac gtaccaagtg
      361 taattccgga aggtttaaca ttaggttgta tccctgatag agaattacct tttgatgcgt
      421 atatttctaa aacacataca ccactatctc aattgccaga aggtagtatt attggtacta
      481 gttcattacg tcgtggtgca caaatattat ctaagtatcc taatttagag attaaatgga
      541 ttagaggtaa tatagataca cgattagaaa agttacaaac tgaagattat gatgcgatta
      601 ttttagctgc agctggttta agaagaatgg gctggtcaga tgatatcgta acatcttatc
      661 ttgatagaga tacattgtta cctgcaatcg gacaaggtgc tttagggata gaatgtcgta
      721 gtgacgatga tgaactatta tcattattaa gcaaagtaca taatgatgag gttgcaaaat
      781 gtgtgactgc tgaacgaacg tttttagcag aaatggatgg tagttgtcag gtgccaatcg
      841 caggatatgc tacaatctca gatcaaaaag aaatcgaatt tacaggttta attatgaccc
      901 cagatggtaa agaacgattt gaatatacaa tgaacggaac agatccggtt gagttaggca
      961 aaacagtgag taacaaatta aaagagcaag gtgcttatga aattataaaa cgcttaaatg
     1021 aacaacatta aataatggag tgacgtttaa atatgaagcc agttgtagtt atgacacaaa
     1081 caaatgacat gcaaagtgat ttagtatcaa ttattcataa accctttatt gatataaagc
     1141 cactaaattt tgatattcat ttgttaaatc aacgctatga ctggcttatt ttttcatcta
     1201 aaaatgctgt gaaattcttt tataaatatc taaaaggaat taatgttgat aacattgctg
     1261 tgataggaag taagacagca caatattgtg aatcacttgg cattcgagtt gattttatgc
     1321 caaatgactt ttctcaagaa ggatttttaa aatcatttaa tcaaactaac caaaaaatac
     1381 ttttgccttc gagtgaattg gcgagaccat tgttatcagc agcgttatct aaagataatg
     1441 aagttgttaa aatagattta tatacttcag tgccta
//
LOCUS       DAPIDM010000239         1471 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000239 DAPIDM010000000
VERSION     DAPIDM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.247"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(170..607)
                     /gene="mnhG2"
                     /locus_tag="RJE42_002712"
     CDS             complement(170..607)
                     /gene="mnhG2"
                     /locus_tag="RJE42_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDV5878063.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            complement(582..884)
                     /gene="mnhF2"
                     /locus_tag="RJE42_002713"
     CDS             complement(582..884)
                     /gene="mnhF2"
                     /locus_tag="RJE42_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDV5878064.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGYVFNGNNKRNL"
     gene            complement(881..1363)
                     /gene="mnhE2"
                     /locus_tag="RJE42_002714"
     CDS             complement(881..1363)
                     /gene="mnhE2"
                     /locus_tag="RJE42_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDV5878065.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
BASE COUNT          540 a          248 c          203 g          480 t
ORIGIN      
        1 ctaattgttt gtataaataa acaacttgct ttcacataac aacattaact tataataaaa
       61 aaatgagcac cttaaaatcg actaaccaat ttcaaagtac tcttttaatg attaattttg
      121 aaaacaaatc ttcaaaagca ttgttatgct taacaattta ggcaacactt caatcgtttt
      181 gataccattt cttacgatgc tcttctcgtt ttttagcacg taattgtaat gcttctgtag
      241 agttttgttc atttgaactt aataatattg atgcatgtgt gtgatcatca ttttttcgat
      301 acatataagc gccgttgcga taagcagcgc gagcgactaa gtgcatgccg acgggtgaag
      361 ttaaattaat aaaaacaagt gacagtaata aacgcacact gaaaaatcct gtattcacaa
      421 taaaataaat taatacaccg attaaagtta ataacacgga taaagtcgaa ctttttgttg
      481 cagcatgact tcttaagaaa acatcttgga attttacgat acctattgca ctaataagag
      541 caataaaact acctaacaac aacatcacag ctgcaataag actaaagatt tcttttgtta
      601 tttccattaa acacataccc cccaccaata aatcttgaaa tcgatacaga gcttacgaaa
      661 gatataatgg caatgagcat gattgaatct aggaaagaaa cggtgcccat tagtacactt
      721 aagacaccca caatagacat gactacagca cttgttgtat caaatgtaac gacacgatct
      781 gctgttgtag gtcccttgat taatctaaat aaacagatga ttaatgcaat tccaaaaata
      841 atgagtgaac taataatcat aatatgtgtg attgtttgta tcatcgcgac acctccaata
      901 ttaagtcttc ataatgctta atacttctta acaaactatc tttttctttt tctgacacgt
      961 cgatactatg aataaaaaac tttttagagt cttgggaaat tcgtattact gtagaccctg
     1021 gagttataat aattaaaatt gttaaaaatg ttattgccca gtcacttgtt agtcttgttt
     1081 catatgaaag taatccaggg ttcatatctt ttgttttaaa aagaatataa ttaatcgtgc
     1141 taatgctaga tgttattaat tggtataaat aaacacctaa aaatttaata gctacccaaa
     1201 tttttctaac ataaaaatca tcgctgaaaa acctgtgcaa tatataaatg acaattaaac
     1261 caattagata tccagaaaag aaagtcgaga atttaaaatg atcttcatct tgaaataaaa
     1321 cccataagaa tgcaatgata atatttaaaa caatttgatt catttattcc tctcctttca
     1381 aatgtggatt aacaagcttt tgatataatt gatcgttcgt attaagctct gttgcatcat
     1441 tagttatttt taacagcaca ggcgctgcaa t
//
LOCUS       DAPIDM010000240         1446 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000240 DAPIDM010000000
VERSION     DAPIDM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.248"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>1446
                     /locus_tag="RJE42_002715"
     CDS             <1..>1446
                     /locus_tag="RJE42_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV5878066.1"
                     /translation="NLIKFIDKREEWEKDKWDIRNLEKYGLSYNKTLTGNYLNFEKIE
                     SIKMRELAKKYLKNRLITGDIAFATARFYIRVLTRFFQNISKNKETRNSLNELDRCHI
                     EAYIEFLFEYAANKHLQSTKNFVREELKTIRRFLNDIITQNYAIAPYQDIRFLIYPQD
                     LPKHEKKNSSQIDYIPDFVLEQLFEHINDLHKDLIPVVWIAFKTGLRISDVLTLQNNC
                     LAKVNGKYSIITDIAKTFVKGHRIPIDNKLADIIAVLIADSKSKSTKDNNPNNYIFAI
                     YKGKRKGMPFTQHMVRAHLNHLSKTKNIIDEQGEIFHFKTHQFRHTYAVKLLNGGADI
                     LTIQELLAHSSPEMTLRYAKLLDDTKRKAFESVIDQGAFSFDVDGKIKNIQHSSELSE
                     KALNSLWQEHKLNAMDNPYGTCHARLSGDCPYMEAPPCLTCNSGKPCKDLAIGFSDLD
                     VEKYELHIKSTVKSIELAKNNNRQDMVEKHI"
BASE COUNT          592 a          175 c          232 g          447 t
ORIGIN      
        1 taatctaatt aagttcattg ataagcgaga agaatgggaa aaagataaat gggatattag
       61 aaatttagaa aaatatggat tgagttataa taaaacacta actggcaatt atttaaactt
      121 tgaaaaaatt gagtcaatta aaatgcgaga attagcaaaa aaatatttaa aaaatcgttt
      181 gataactggt gatatagcat ttgctacagc aaggttttat ataagagttt taactaggtt
      241 ttttcaaaat atatctaaaa ataaagaaac aaggaatagt ttaaatgaac tagatagatg
      301 tcatatcgaa gcatatatag aatttttgtt tgaatatgca gctaataaac atttgcaaag
      361 tactaagaat tttgtgagag aagaattaaa aacaattaga aggttcttaa acgatattat
      421 aactcaaaac tatgctatag caccgtacca agatatacga tttttaattt atcctcaaga
      481 cttacctaaa cacgaaaaga aaaatagtag ccaaattgat tatatcccag attttgtatt
      541 ggaacagctt tttgagcata taaatgattt gcataaagat ttaatacctg tagtttggat
      601 agcttttaaa acaggattaa gaatttctga tgtattgaca ttacaaaata attgccttgc
      661 aaaagttaat ggaaagtatt caataatcac agatattgct aaaacttttg ttaaaggaca
      721 tagaatacca attgataata aattagctga tataatagca gttttaatag ccgattctaa
      781 aagtaaaagt acaaaagata ataatcctaa taattatatt tttgctattt ataaaggaaa
      841 aagaaaggga atgcccttta cacaacatat ggtcagagca cacttaaatc atttatcaaa
      901 gactaaaaat attattgatg aacaaggaga aatcttccat ttcaaaactc atcaatttag
      961 acatacctat gcagtaaaat tgttaaatgg tggagcagat atattaacga tacaagaatt
     1021 attagcacac tcctcaccag aaatgactct aagatatgct aagttacttg atgatacaaa
     1081 gagaaaggct tttgaatcag ttattgatca aggagctttt agttttgatg ttgacggtaa
     1141 aattaaaaat atacagcata gtagtgaact atctgaaaaa gcattgaatt ctttatggca
     1201 agaacataaa ttaaacgcaa tggacaatcc ttatggcact tgtcatgcta gattgagtgg
     1261 tgattgtcca tacatggaag ctccgccatg cctgacttgt aattctggga aaccatgtaa
     1321 ggatttagct attggatttt ctgatttaga tgttgaaaaa tatgaattac atatcaaatc
     1381 aacggtcaaa tctatagaat tagctaaaaa taataataga caagatatgg ttgaaaagca
     1441 tataaa
//
LOCUS       DAPIDM010000241         1438 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000241 DAPIDM010000000
VERSION     DAPIDM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.249"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>1438
                     /locus_tag="RJE42_002716"
     rRNA            <1..>1438
                     /locus_tag="RJE42_002716"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          422 a          277 c          416 g          323 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctgg aaagatgaat caaagaaggt
      361 aataatcctg tagtcgaaaa tgttgtctct cttgagtgga tcctgagtac gacggagcac
      421 gtgaaattcc gtcggaatct gggaggacca tctcctaagg ctaaatactc tctagtgacc
      481 gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg agtgaaatag
      541 aacctgaaac cgtgtgctta caagtagtca gagcccgtta atgggtgatg gcgtgccttt
      601 tgtagaatga accggcgagt tacgatttga tgcaaggtta agcagtaaat gtggagccgt
      661 agcgaaagcg agtctgaata gggcgtttag tatttggtcg tagacccgaa accaggtgat
      721 ctacccttgg tcaggttgaa gttcaggtaa cactgaatgg aggaccgaac cgacttacgt
      781 tgaaaagtga gcggatgaac tgagggtagc ggagaaattc caatcgaacc tggagatagc
      841 tggttctctc cgaaatagct ttagggctag cctcaagtga tgattattgg aggtagagca
      901 ctgtttggac gaggggcccc tctcgggtta ccgaattcag acaaactccg aatgccaatt
      961 aatttaactt gggagtcaga acatgggtga taaggtccgt gttcgaaagg gaaacagccc
     1021 agaccaccag ctaaggtccc aaaatatatg ttaagtggaa aaggatgtgg cgttgcccag
     1081 acaactagga tgttggctta gaagcagcca tcatttaaag agtgcgtaat agctcactag
     1141 tcgagtgaca ctgcgccgaa aatgtaccgg ggctaaacat attaccgaag ctgtggattg
     1201 tcctttggac aatggtagga gagcgttcta agggcgttga agcatgatcg taaggacatg
     1261 tggagcgctt agaagtgaga atgccggtgt gagtagcgaa agacgggtga gaatcccgtc
     1321 caccgattga ctaaggtttc cagaggaagg ctcgtccgct ctgggttagt cgggtcctaa
     1381 gctgaggccg acaggcgtag gcgatggata acaggttgat attcctgtac cacctata
//
LOCUS       DAPIDM010000242         1425 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000242 DAPIDM010000000
VERSION     DAPIDM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.250"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            265..>1425
                     /locus_tag="RJE42_002717"
     CDS             265..>1425
                     /locus_tag="RJE42_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDV5878067.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANFVAEIGSGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKDDKLYGRG
                     TTDMKGGLMALVIALIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LMIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYAE
                     LKKNDTKHELDVAPMFKSLIGKDISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINNVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVDKDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKD"
BASE COUNT          515 a          218 c          252 g          440 t
ORIGIN      
        1 gtaaatcgca ttatttagta gttaatcacc atggatctat atgttataca tgcttaaact
       61 aaattttttt catacaatcc gactggacga ttcacaatga aaaaaacact tactatcagt
      121 gattattaga ctactgatac aaggtgcata ttttaagatg taatcatgaa catagcacgt
      181 cactttgtta cttaaaatta atgtttaaat acaataatta tgtttatata aatatatata
      241 ctactttgaa aaggtgtgag cttaatgaca acttttagtg aaaaagaaaa aattcaatta
      301 ctagcagata ttgttgaact acaaactgaa aataataatg aaatagacgt ttgtaattat
      361 ttaaaagatt tattcgacaa gtacgatatt aaatctgaaa ttttgaaagt taatgaacac
      421 cgcgccaatt tcgttgcaga aatcggtagt ggctctccca tacttgcatt gagcggtcat
      481 atggatgttg ttgatgcagg aaatcaagat aattggactt atcccccttt tcaactcaca
      541 gaaaaagatg acaaattata tggtcgaggc acaacagata tgaaaggcgg tctgatggct
      601 ttggtcatcg cactcattga attaaaagaa caaaaccaat tacctcaagg aaccattaga
      661 ttactcgcta ctgctggtga agagaaagaa caagaaggcg ccaaattatt agcagataaa
      721 ggctatttag atgatgttga tggtctaatg attgctgaac caactggatc tggaatttat
      781 tatgcacata aagggtctat gtcatgtaaa gtaactgcaa ctggtaaagc tgtccatagc
      841 tcagttccat ttattggtga caatgcaatt gatacactgc ttgaatttta taatcaattt
      901 aaagaaaaat atgcagagct taaaaaaaat gatactaaac atgagttaga tgttgctccc
      961 atgttcaaat cattgatagg aaaagatatt tctgaagagg atgcaaatta tgcatctggt
     1021 cttacagctg tatgttcgat tataaatggc ggtaaacaat ttaactctgt cccagatgaa
     1081 gcttcacttg aatttaacgt aagaccagtt cctgagtatg ataacgactt tatagaatcg
     1141 tttttccaaa atatcattaa taatgtggat agcaataagc tttcactcga tattccaagt
     1201 aaccatcgac ctgtaacaag cgataaaaat agcaaattaa ttactacgat taaagatgta
     1261 gcttctagtt atgtagacaa agacgatata tttgtttcgg cgcttgtagg tgcaacggat
     1321 gcttccagct tcttaggaga taataaggac aatgttgatt tagccatttt tggaccaggt
     1381 aatccattaa tggcacatca aatcgatgaa tatattgaaa aagat
//
LOCUS       DAPIDM010000243         1400 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000243 DAPIDM010000000
VERSION     DAPIDM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.251"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            194..1164
                     /locus_tag="RJE42_002718"
                     /pseudo
     CDS             194..1164
                     /locus_tag="RJE42_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          555 a          179 c          231 g          435 t
ORIGIN      
        1 taatggctat aaactatttt atatctatct atgttttaac cacttaaatt attagacaaa
       61 acaattttct taaattatca attaaagtat ttaaaaatat tgatggttaa tgtaaaatat
      121 caatataaat aagttttata aatttatgta tgtttatttg attcaaacaa aataacttaa
      181 gaggagaaag tttatgaaaa agacactggg atgtttactt ttaattatgc ttttagtcgt
      241 agcaggttgt tcttttggtg ggaatcataa attatcatca aagaaatcag aagaatcaaa
      301 acaagaaact gtaaaaaaag aatcggaaga agagaaagac ccaaaagttg cacactataa
      361 aaaaataagg aaaaaatagc gaaagttaaa gcagtacctg ttttagaaaa gatagaacca
      421 tttataaccg aaaagacatt tatgaatagc acaggtatgc aagggtggaa agattataag
      481 tctttaatga atcaagtgaa gttagcagat tataatttta caaaagagtc taaaggtgct
      541 tcaatggaag atgttgataa gttttttaaa aataaaaaag gtgtaaaacg taaagaagtt
      601 actacttatg atggtttgaa acaagttaac tattggtatg tagataaaag tggtaaaaaa
      661 attggtggtt cagacacacc tgttttttat gctgaaattt taacgaaata taaagatgga
      721 aagttgattt atgcatcagt tgaacctgga tcatattcat attcaaataa gaatgcggta
      781 aatctagata aagttgaaga acttgatgat ttaagtatgt tctctaatct gaaagatcct
      841 aaaccagtac catatagtgt tgctcaaatg gaaatatcaa gcgtcccagt tacaagtgta
      901 tctttcgtaa caaagggagg gaatcataaa gatactaatc cagagaaaga acaagttgat
      961 atgccacaac tagcttattt aactgtttca ccacagctat accatgataa agagcatcca
     1021 gatccgcata taattggatt agttgccttg ccatacttaa atgcatctag agattttggg
     1081 aacgcgcatt actctgtttt aaataatctt tcaaaagaaa tgaaagagaa attagcgagt
     1141 cgttcattgg accttaataa atagtgtact acattaaata atcgcaataa taatcccgat
     1201 aaacaatcag cattactact tatcatatag agttcgtaat aactataact ctatgattcg
     1261 caaataataa atgattgtca tcgggattta tttttatcaa tttataaagt gacattacct
     1321 tgttcatcag caggtttgaa aacattaatc actgcactaa taattgctaa aatgtgtggg
     1381 atacctgtcc aacagaatat
//
LOCUS       DAPIDM010000244         1390 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000244 DAPIDM010000000
VERSION     DAPIDM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.252"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(28..>1390)
                     /locus_tag="RJE42_002719"
     rRNA            complement(28..>1390)
                     /locus_tag="RJE42_002719"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          308 a          401 c          308 g          373 t
ORIGIN      
        1 aagaagatgt tccgaatata tccttagaaa ggaggtgatc cagccgcacc ttccgatacg
       61 gctaccttgt tacgacttca ccccaatcat ttgtcccacc ttcgacggct agctcctaaa
      121 aggttactcc accggcttcg ggtgttacaa actctcgtgg tgtgacgggc ggtgtgtaca
      181 agacccggga acgtattcac cgtagcatgc tgatctacga ttactagcga ttccagcttc
      241 atgtagtcga gttgcagact acaatccgaa ctgagaacaa ctttatggga tttgcttgac
      301 ctcgcggttt cgctgccctt tgtattgtcc attgtagcac gtgtgtagcc caaatcataa
      361 ggggcatgat gatttgacgt catccccacc ttcctccggt ttgtcaccgg cagtcaactt
      421 agagtgccca acttaatgat ggcaactaag cttaagggtt gcgctcgttg cgggacttaa
      481 cccaacatct cacgacacga gctgacgaca accatgcacc acctgtcact ttgtcccccg
      541 aaggggaagg ctctatctct agagttgtca aaggatgtca agatttggta aggttcttcg
      601 cgttgcttcg aattaaacca catgctccac cgcttgtgcg ggtccccgtc aattcctttg
      661 agtttcaacc ttgcggtcgt actccccagg cggagtgctt aatgcgttag ctgcagcact
      721 aaggggcgga aaccccctaa cacttagcac tcatcgttta cggcgtggac taccagggta
      781 tctaatcctg tttgatcccc acgctttcgc acatcagcgt cagttacaga ccagaaagtc
      841 gccttcgcca ctggtgttcc tccatatctc tgcgcatttc accgctacac atggaattcc
      901 actttcctct tctgcactca agttttccag tttccaatga ccctccacgg ttgagccgtg
      961 ggctttcaca tcagacttaa aaaaccgcct acgcgcgctt tacgcccaat aattccggat
     1021 aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt ggctttctga
     1081 ttaggtaccg tcaagatgtg cacagttact tacacatatg ttcttcccta ataacagagt
     1141 tttacgatcc gaagaccttc atcactcacg cggcgttgct ccgtcaggct ttcgcccatt
     1201 gcggaagatt ccctactgct gcctcccgta ggagtctgga ccgtgtctca gttccagtgt
     1261 ggccgatcac cctctcaggt cggctatgca tcgttgcctt ggtaagccgt taccttacca
     1321 actagctaat gcagcgcgga tccatctata agtgacagca agaccgtctt tcacttttga
     1381 accatgcggt
//
LOCUS       DAPIDM010000245         1360 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000245 DAPIDM010000000
VERSION     DAPIDM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.253"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            475..702
                     /locus_tag="RJE42_002720"
     CDS             475..702
                     /locus_tag="RJE42_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878068.1"
                     /translation="MFTKITNQFKEIYNTIEKLLNDKSISNYRINQDTGVSYGGISEL
                     RSGKRKVNNLTLETAEKLYNYQKQLEIMIED"
     gene            1003..1209
                     /locus_tag="RJE42_002721"
     CDS             1003..1209
                     /locus_tag="RJE42_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878069.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGNTKI
                     KDAKFKTIIKLYEYQKLSENNSEC"
BASE COUNT          594 a          137 c          195 g          434 t
ORIGIN      
        1 atatgtataa atggaaatgt ttattacatt acataaaaac ctttaggtgc tcttagatgg
       61 atgttaatca taaaacaaag tgattttgaa aaatataggt tattataatt tagaattgga
      121 aattataaat cttgtcattg gatatgggaa ttcaaacata tcacttacaa caatttgtgg
      181 aatgttaaat aactataaag aggaaaggag aaaaaatgat aagaatggtt aaagaaaaag
      241 aacgaatagc aaaattaaat ttcgaatagc tgtgacgagc ataataattc aagtgcttaa
      301 ttttatcaaa tcgttctttt aaaaaggtaa agaacaaaag acctttacca cattaaatag
      361 ggaggtggta tttatgacaa gagaattaag gaaaaaatta actctctatc ttaacattgc
      421 aactttaata ttatttatta ttaatttaac aagaaaaaaa taaaatattc tttaattttt
      481 acaaaaatta cgaaccagtt taaagaaatt tataatacaa tagaaaaatt actaaatgat
      541 aaatcaatat ctaattatag aattaatcaa gacactggtg tttcttatgg tggtataagt
      601 gaattaagaa gcgggaaaag aaaagtgaat aatttaactt tagaaacagc ggaaaaactc
      661 tataattacc aaaaacaatt agaaataatg attgaagatt aaaataaggc catatctttt
      721 gaaatatcat ggagtgagcc ttttaaatca tctaatttta aagtatatta aatatggata
      781 gcttaatgtg aatataaata aaaaattatc aaataatata atataatata gaatagattg
      841 agacaaggaa ttttatcaat atataataaa aaacgccaat actattaggc attagtttat
      901 gaattaacta gtttggcaac tacaaagaaa caatgtattg ctaaacgtag ttactgcatt
      961 aggataatta taattataaa tagaaagaga gcgtgattaa aaatgaatga gattgaaact
     1021 attataagtg aaatagaaaa gttattaact aacaatacac catatagtat ttcaaaaaac
     1081 tcaggtgtac cacgtcaaac agttactgat ttaaaggtag gtaatactaa aataaaggat
     1141 gccaaattta aaacaataat caagttatac gagtatcaaa agttgtcaga aaataattca
     1201 gagtgctaat aaatatacct tgtttgatta ggtggacaag aaattaaatt tctaatgatt
     1261 tcatggttgt ctgtccatgc tttgtccacc tcaataaaat tatctctagg tttttaaaat
     1321 tgtacatgtt taaacaatca aatgtatgca tttttatatt
//
LOCUS       DAPIDM010000246         1334 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000246 DAPIDM010000000
VERSION     DAPIDM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.254"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            237..989
                     /gene="sarS"
                     /locus_tag="RJE42_002722"
     CDS             237..989
                     /gene="sarS"
                     /locus_tag="RJE42_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDV5878070.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMNDAQQDHAEQLLAQVNQLLADKNHLHLVFE"
BASE COUNT          522 a          186 c          145 g          481 t
ORIGIN      
        1 aatactttat atacaaataa caccgactca cattctataa tatcaatcaa tattcttcaa
       61 tttttcaaat atcgataact atttcttatt taaatatagt gtttgataat gtcatttatt
      121 caaaaacaca aattttaata aaaatatcat attattttta attgtaaatt atggattatt
      181 tttcttatta tattgtgtta tattaaaata aagtgcatat acaaggagat aaaacaatga
      241 aatataataa ccatgacaaa attagagatt ttataatcat tgaagcatat atgtttcgtt
      301 ttaagaaaaa agtcaagcct gaagtcgata tgactataaa agaatttata ttactgactt
      361 atttatttca tcagcaagaa aacacacttc catttaagaa gattgtttca gatttatgtt
      421 ataaacaatc ggatttagta cagcatataa aagtacttgt gaaacattca tatattagta
      481 aagttcgtag taaaattgat gagcgtaata cttacatttc aatatctgaa gaacaacgag
      541 agaaaattgc agaacgtgtt acattgtttg atcaaatcat taaacaattt aatcttgcag
      601 atcaaagtga atcacagatg ataccaaaag atagtaaaga atttctaaac ttgatgatgt
      661 atacaatgta tttcaagaat attatcaaaa agcatctaac attaagtttt gtagaattca
      721 caattctagc tattatcact tctcaaaata aaaacatcgt tcttcttaaa gatttaattg
      781 aaacaatcca ccataaatac cctcaaactg ttagagctct caataattta aaaaagcaag
      841 gctatctaat aaaagaacgc tcaactgaag atgaaagaaa aattttaatt catatgaatg
      901 acgcgcagca agaccatgct gaacaattat tagctcaagt gaatcaatta ttggcagata
      961 aaaatcattt acatcttgtt tttgaataat atctctatta cgcaagtgtg ctgtattcta
     1021 aagtgcactt gtgttttcta ttttttaata aaacctcagc acattatgaa caactttcta
     1081 ttttctatat ctcttaaaac catttccgaa attaaacctc agcacattca aaattccatt
     1141 ttattcttaa aaatattttt taactcatat gtaataaacc gctttcatta taaaaaatat
     1201 ctatatattt tatctgtttt tattaatcgg aatagcgtga ttttgcggtt ttaagccttt
     1261 tacttcctga ataaatcttt gaacaaaatt tttattttat aagttgtaaa acttaccttt
     1321 aaatttaatt ataa
//
LOCUS       DAPIDM010000247         1309 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000247 DAPIDM010000000
VERSION     DAPIDM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.255"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..231
                     /locus_tag="RJE42_002723"
     CDS             <1..231
                     /locus_tag="RJE42_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HDV5878071.1"
                     /translation="ILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPSSI
                     EDLIADGFIKEAQKTCKSGETITISNGEAVAN"
     gene            209..655
                     /locus_tag="RJE42_002724"
     CDS             209..655
                     /locus_tag="RJE42_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDV5878072.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISLITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            642..941
                     /locus_tag="RJE42_002725"
     CDS             642..941
                     /locus_tag="RJE42_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDV5878073.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            859..>1309
                     /locus_tag="RJE42_002726"
     CDS             859..>1309
                     /locus_tag="RJE42_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV5878074.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDILEDFKGVDRNDIEIRQQRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITI"
BASE COUNT          542 a          149 c          209 g          409 t
ORIGIN      
        1 attttaatca ttccaaatat tgctaaacaa actgctcaca tacaatcaac aggttgtaat
       61 gcacaggtaa aaatggttaa tagtcaaatt gaagcgtatg cattgaaaca taatagaaat
      121 ccatcgtcta ttgaagactt aattgcagat ggttttataa aagaagcaca aaagacatgt
      181 aaatcaggag agacaataac aattagtaat ggagaagcag ttgcaaatta gaaagcagtc
      241 agcatttact atgattgaaa tgcttgtggt aatgatgtta atcagtatat ttttactttt
      301 gacaatgaca tctaaaggat taagcaatct tagagtaata gatgatgagg caaatatcat
      361 ttctcttatt actgaattga attatattaa gtcgcaagct atagcaaatc aaggatatat
      421 caatgttaga ttttatgaaa acagtgacac aattaaagta atagagaata ataaaatacg
      481 atttctaaaa ttaaaagtag gcaaaataat taatgttgca aaagttgata ttattgcctt
      541 tgataaaaaa gggaatatca ataaatttgg tagcataaca attgacaata acaattcaat
      601 ttatagaata atattccata ttgaaaaagg aagaattcgt tatgaaaagc tataagtgta
      661 aaggatcatt cttaatagat agtatggctg gatttttgct tattggattg attacattac
      721 tattgatacc aatgatgaat caaatgcaag cgagtataaa ccataaacta caaacaattg
      781 atgcttctaa agtaattttg acgactgtat ctaaaattaa taaagaagaa cttaagaagg
      841 gggtaactat agggaaatat gatattaagc aaagtgacca acaaatttgt gctatttcaa
      901 aaaataccac ttcttatcaa aagacatgta tacagtatta atgtcaaagc tttttcgctt
      961 attgaaatgt tagtagcgat gatggttata agcataactt tactaattgt tccagactta
     1021 attagactta gcaaaacttt tctaattgaa agtagggaat taacaactgt agatttcgaa
     1081 tttttctcaa gagatattct agaggatttt aaaggtgtag atagaaacga tattgaaatt
     1141 aggcaacagc gtatcatttt acataaaggt gaagaaatga tcgaatacaa attaataaat
     1201 aataaaatta ttaaagttgt aaatgacaga ggaaatataa cattgattaa taatgttaca
     1261 gcatttactg caaatatcta ctataaatct attattaaaa taacgataa
//
LOCUS       DAPIDM010000248         1301 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000248 DAPIDM010000000
VERSION     DAPIDM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.256"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..975)
                     /locus_tag="RJE42_002727"
     CDS             complement(<1..975)
                     /locus_tag="RJE42_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDV5878075.1"
                     /translation="MNGDGKMTIDKKKFIEKYVKALESNTAAIFAGAGLSCGAGYVNW
                     KTLLNEAAEELELNIDKEEHDLVGLAQHYINKKRSRGNLNTVIMEQFSTRAELTENHK
                     LLAKLPIDTYWTTNYDCMIEKALDSEGRIVDVKRNQNQLTVSVADKDATVYKMHGDID
                     SIDKIILTRDDYEKYNLTHPKFREILEGDLLSKTFLFIGFSFTDPNISYILSRIRLVL
                     EDNTRPHYCILKEVEEKEYDNTEEFMYAKIKQQLHIEDLSRFSIETLLVKDYIEITEI
                     LESIYKRYRRKTIFISGSATEYLPFNSETGKYFLHQLSKKLVENDFKLV"
     gene            complement(1035..>1301)
                     /locus_tag="RJE42_002728"
     CDS             complement(1035..>1301)
                     /locus_tag="RJE42_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDV5878076.1"
                     /translation="YDALASHDTAEHVLGDDTLKIIAHELTQSIKENMSIDWNLCESA
                     RAKMRVIVRRLLKKYGYPPEISKQAVETVIEQAELMSEQLAMEL"
BASE COUNT          391 a          228 c          152 g          530 t
ORIGIN      
        1 aactaattta aaatcatttt caactaattt tttacttagt tgatgtaaaa aatatttccc
       61 tgtttctgaa ttaaaaggta aatattctgt agcacttcct gatataaaaa ttgtttttct
      121 tctatatctt ttataaatac tttctaatat ttctgtgatt tcaatataat ctttaactaa
      181 taaagtttct attgaaaatc ggcttaaatc ttctatatga agttgttgtt ttatctttgc
      241 atacataaat tcttctgtat tatcatattc cttttcttct acttctttta aaatacaata
      301 atgtggtcta gtattgtctt ctaacactaa tcttattctc gataagatat aactaatatt
      361 tggatctgta aagctaaaac ctatgaatag aaaagttttt gaaagtaaat ctccttccaa
      421 tatttcacga aactttggat gagttagatt atatttttca taatcatctc tagttaaaat
      481 aatcttgtct atactgtcta tatccccatg cattttataa actgtagcat ccttatcggc
      541 tactgaaaca gttaattgat tttgatttct tttcacatct acaattctgc cctcagaatc
      601 aagagctttt tcaatcatac aatcataatt agttgtccaa tatgtatcaa tgggaagttt
      661 tgctaaaagc ttatgatttt ctgttaattc tgctcttgtt gaaaattgct ccattattac
      721 tgtatttaga tttccacgac ttcttttctt attaatataa tgttgtgcta atcctacaag
      781 atcatgttct tctttatcta tatttaattc taattcttct gctgcttcat taagcaatgt
      841 cttccaatta acataacctg ctccacaaga taatcctgct ccagcaaaaa tagccgctgt
      901 attactttct aaagctttta catatttttc aatgaatttt ttcttatcta tagtcatttt
      961 gccatctcca ttcataaaaa attatataaa agcacaataa agagaatttc taaattgtgc
     1021 tttaaatcta gttcttataa ttccatcgcc agctgctctg acatcagctc tgcttgttca
     1081 atgacggttt ctactgcttg tttactgatt tctggtggat atccgtactt tttcaatagg
     1141 cgtcttacga taactcgcat ttttgcacgt gctgattcgc ataagttcca atcaatactc
     1201 atattttctt taatagactg agttagttca tgagcaatga tttttaatgt atcatcacct
     1261 agaacatgct cagctgtatc atgtgaagct aaggcatcgt a
//
LOCUS       DAPIDM010000249         1219 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000249 DAPIDM010000000
VERSION     DAPIDM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.257"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            203..>1219
                     /locus_tag="RJE42_002729"
     CDS             203..>1219
                     /locus_tag="RJE42_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDV5878077.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSNDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQNMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVFVFNKEISN
                     KHIKL"
BASE COUNT          403 a          178 c          178 g          460 t
ORIGIN      
        1 attgtctgtt gaaattggga atccaatttc tctttgttgg gccccacccc aacttacatt
       61 gtttgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga attgaaaagt
      121 aatacacatc agtaacattt ctattttcat ttatgatatt atctaattat taatttataa
      181 tataaaggag tatgcaatta aaatgaacaa agtccacaaa cctttatatt tttatttaat
      241 gctattcttc tctacaacta tcataggtgc tcttttattg tatttacctt tcactggtaa
      301 aaagccaata tcatttttag atgccctatt tatagcttca agtgcattta cagttactgg
      361 cttgtcccca gttgatatag gatcacagtt taatatactt ggtgaaatag taatactatt
      421 attaattcaa ataggtggtc tgggtatcgt gaccgtaacc ctattgacac tagtattttt
      481 aaatagaaag atatcaatga aaaatagatt cttgattatg gttacatgga atattgacga
      541 acctggtggt gttattaagc taattaaaca cttggctatt tatagtttag tcactgaatt
      601 aattggtatg atttgtttgt gtttatcttt tataccaaaa tttggtatag gcaaaggatt
      661 atttttaagc ttattcacat cagtatcagc ttttaataat gctggatttg ccctttttaa
      721 gaataactta atagattatt ctaatgatcc aattgtcatt atcacaatct caatactcat
      781 aatatttgga ggtattggac attttgtcgt aatagacttt attaattgta aaaaattgag
      841 taaattatct ttacattcta aattagtctt aactacaact agtatcctaa taattatagg
      901 agctattaca ttctttttat tagaacagtt taatactatg caaaatatgg gactagttga
      961 aaaaatcgga aattcttttt tccaatcagt aacaacacga acagcgggtt ttaacagtat
     1021 tgatatagca agcattaaca aatctaccgc cttaatgtta atgctactaa tgtttattgg
     1081 tggtgcccct ctcagtgcag ctggaggaat taaaataact acttttgcag tagcgtttat
     1141 ttttgtacta aattatatac gtaaagaaaa taatgttttc gtattcaata aagaaatatc
     1201 taacaaacat ataaaacta
//
LOCUS       DAPIDM010000250         1150 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000250 DAPIDM010000000
VERSION     DAPIDM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.258"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..1094
                     /locus_tag="RJE42_002730"
     CDS             <1..1094
                     /locus_tag="RJE42_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDV5878078.1"
                     /translation="EFEKNVENIESKHLDLKRFDEDKDYEARVKLNELENKVLMLGQA
                     FPDKVDARESLYNKLDMIVGLDNDEIEERHPQNERLLKERVEDLETIIDEFFNDIGEN
                     RPENIAPLTNNEHKNKEMITKLKSDTEAAKSDESKRSKRSKRSLNTQNHKSKINEVTA
                     EQKAEYERKAEERKEKFLAKNKDNPVVSLIDEEDDNENENDKQLVVSAPTNKPTTLPT
                     YTETTTQVPMPTVERQTQQQIVYKAPKPLAGLNGESHDFTTTHQSPTTSNHTHNHLIE
                     IEETSALPGRKTGSLVGLSQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDR
                     LNAQQKVNTLSAGHQKRFNKQINKVYNGK"
BASE COUNT          511 a          162 c          200 g          277 t
ORIGIN      
        1 aagaattcga aaaaaatgta gaaaatattg aaagtaagca tcttgattta aaaaggtttg
       61 atgaagacaa agattatgaa gcaagagtta aattaaatga attagaaaat aaagttttaa
      121 tgttaggtca agcttttcct gataaagtag atgctagaga aagtctttat aataaattgg
      181 atatgattgt tggtttagat aatgatgaaa ttgaagaaag acatccgcaa aatgaaaggt
      241 tattaaaaga acgagttgaa gatttagaaa caattatcga tgaattcttt aatgacatag
      301 gtgaaaatag accggagaat atagcaccat taactaacaa tgagcataaa aataaggaaa
      361 tgattacaaa gctaaaatct gacactgaag cagcaaaaag tgatgaatca aaaagaagca
      421 aaagaagtaa aagaagttta aatactcaaa atcataaatc taaaataaac gaagtaactg
      481 cagaacaaaa agcagaatac gaaagaaaag ctgaagaaag aaaagaaaag tttttagcta
      541 agaacaaaga taatccagtg gtttcactaa tcgatgagga agacgacaac gaaaacgaaa
      601 acgacaagca acttgtggtt tctgcaccaa caaataaacc aacaacactg cctacataca
      661 ctgaaacaac gacacaggta ccaatgccta cagttgagcg tcaaactcag caacaaatcg
      721 tttacaaagc accaaaacca ttagctggat taaatggtga aagtcatgat ttcacaacaa
      781 cgcatcaatc accaactact tcaaatcaca cgcataatca tcttattgaa attgaagaaa
      841 catctgcttt acctggtaga aagacgggtt cattggttgg tttgagtcaa attgattctt
      901 cacatttaac tgaacgtgag aagcgcgtga ttaagcgtga acacgtgaga gaagctcaaa
      961 agttagttga taattataaa gatacacata gttataaaga ccgattaaat gcacaacaaa
     1021 aagtaaatac tttaagtgca ggtcatcaaa aacgttttaa taaacaaatt aataaagtat
     1081 ataatggcaa ataattaatg catggctgca aaacaaataa tgagtttgcc gtaaaaataa
     1141 caacatttta
//
LOCUS       DAPIDM010000251         1117 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000251 DAPIDM010000000
VERSION     DAPIDM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.259"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>1117
                     /gene="tet"
                     /locus_tag="RJE42_002731"
     CDS             <1..>1117
                     /gene="tet"
                     /locus_tag="RJE42_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HDV5878079.1"
                     /translation="IGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILI
                     FGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHY
                     IHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNW
                     TFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPY
                     MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLT
                     IAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSE
                     GTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVA"
BASE COUNT          330 a          125 c          200 g          462 t
ORIGIN      
        1 gataggaaca gcagtatatg gaaaattatc tgattatata aatataaaaa aattgttaat
       61 tattggtatt agtttgagct gtcttggttc attgattgct tttattggtc acaatcactt
      121 ttttattttg atttttggta ggttagtaca aggagtagga tctgctgcat tcccttcact
      181 gattatggtg gttgtagcta gaaatattac aagaaaaaaa caaggcaaag cctttggttt
      241 tataggatca attgtagctt taggtgaagg gttaggtcct tcaatagggg gaataatagc
      301 acattatatt cattggtctt acctacttat acttcctatg attacaatag taactatacc
      361 ttttcttatt aaagtaatgg tacctggtaa atcaacaaaa aatacattag atatcgtagg
      421 tattgtttta atgtctataa gtattatatg ttttatgtta tttacgacaa attataattg
      481 gactttttta atactcttca caatcttttt tgtgattttt attaaacata tttcaagagt
      541 ttctaaccct tttattaatc ctaaactagg gaaaaacatt ccgtttatgc ttggtttgtt
      601 ttctggtggg ctaatatttt ctatagtagc tggttttata tcaatggtgc cttatatgat
      661 gaaaactatt tatcatgtaa atgtagcgac aataggtaat agtgttattt ttcctggaac
      721 catgagtgtt attgtttttg gttattttgg tggtttttta gtggatagaa aaggatcatt
      781 atttgttttt attttaggat cattgtctat ctctataagt tttttaacta ttgcattttt
      841 tgttgagttt agtatgtggt tgactacttt tatgtttata tttgttatgg gcggattatc
      901 ttttactaaa acagttatat caaaaatagt atcaagtagt ctttctgaag aagaagttgc
      961 ttctggaatg agtttgctaa atttcacaag ttttttatca gagggaacag gtatagcaat
     1021 tgtaggaggt ttattgtcac tacaattgat taatcgtaaa ctagttctgg aatttataaa
     1081 ttattcttct ggagtgtata gtaatattct tgtagcc
//
LOCUS       DAPIDM010000252         1113 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000252 DAPIDM010000000
VERSION     DAPIDM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.260"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..160
                     /locus_tag="RJE42_002732"
     CDS             <1..160
                     /locus_tag="RJE42_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDV5878080.1"
                     /translation="TKKAPILKLHIDGDIKGSSVGYKKIEYKFSKVKDQETTLRDYLN
                     FGPSDEDS"
     gene            208..990
                     /locus_tag="RJE42_002733"
     CDS             208..990
                     /locus_tag="RJE42_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5878081.1"
                     /translation="MGYLKRFALYISILVLIVMVAGCGKGNETKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFAKSNKPGEIDDEGMVLFLNR
                     NTKKATGYYYMNKAHDEFNEKDQQKKYHVELKNNKIVLLDNVEDPNLKNKIENFKFFS
                     QYADFRDLKNYKNGNISSTDNVPSYDAEYKMSNTDKNVKKLREVYPITTKKSPVLKLH
                     IDGDIKGSSVGYKNIEYNFSKVKDQETAVRDFVNFGPSDGDS"
BASE COUNT          483 a          109 c          212 g          309 t
ORIGIN      
        1 aacgaaaaag gctccaatat taaagttgca tatagatggt gatataaaag gaagttcagt
       61 tggatacaaa aaaatagaat ataaattttc aaaagttaaa gatcaagaga caacattaag
      121 agattatttg aattttggac cgtctgatga agatagctaa agataattag atgtagaaat
      181 aattctaaaa cagagagacg tgaaacgatg ggatatttaa aaaggtttgc attgtacata
      241 agtatcttgg ttttaatagt tatggtagca ggttgtggca aaggtaatga aacaaaagaa
      301 gattcaaaag aagaacaaat caaaaagagc tttgcgaaaa cgttagatat gtatccaatc
      361 aaaaatcttg aagatttata tgacaaagaa ggatatcgag atggcgaatt taaaaaaggc
      421 gataagggta catggacttt actcacaagt tttgctaaaa gtaacaaacc aggcgagata
      481 gatgacgaag gtatggtttt attccttaat agaaatacaa agaaggcaac aggatattat
      541 tatatgaata aagctcatga tgaatttaat gaaaaagatc aacaaaaaaa atatcatgtt
      601 gaacttaaaa ataataaaat agttcttttg gataatgtag aagatccaaa tctcaaaaat
      661 aaaatagaga attttaaatt ctttagtcag tatgctgatt ttagagatct taaaaattat
      721 aaaaatggaa atatatcaag tacagataat gtgccaagct acgatgcaga atataaaatg
      781 agtaatacgg ataaaaatgt aaaaaaactt agagaggttt atccgattac aaccaaaaag
      841 tcgccagtat taaaattaca tatagatggc gacataaaag gaagttcagt tggttataaa
      901 aatatagaat ataatttttc aaaagtgaaa gatcaagaaa cagctgtaag agattttgtg
      961 aattttggac catctgatgg agatagctaa agtttattaa gtgcagaaat aattctaaaa
     1021 cagagagacg tgaaacgatg gaatatttaa aaaggattgg actgtacata agtattttta
     1081 ttttaatagc tgttatagca ggttgtggca aag
//
LOCUS       DAPIDM010000253         1070 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000253 DAPIDM010000000
VERSION     DAPIDM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.261"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            35..220
                     /locus_tag="RJE42_002734"
     CDS             35..220
                     /locus_tag="RJE42_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878082.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            731..>1070
                     /locus_tag="RJE42_002735"
     CDS             731..>1070
                     /locus_tag="RJE42_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878083.1"
                     /translation="MFKVNYSILSYYPDIYLHSNLAVGVSFEIIGESYHKNEIKFITQ
                     RRKISSFDDEIDNLVLINMFLDGLKCEFEQTNQSIKNYKKKFVNNFYFENIEYKMFDS
                     LKEVNNFIEKT"
BASE COUNT          438 a          108 c          164 g          360 t
ORIGIN      
        1 tagtaaacat tgtacagata tggagatgaa aaaaatgtca gaatacaaga aaaagataat
       61 tgaattaatt gaaagtaatt taacaggata tgaaatttct aaaaaaactg gagtttctca
      121 atatgtactt tcacaattaa gacagggcaa acgcgaagta gataatctaa ccctgaatac
      181 aacagaaaaa ttatatgaat atgccaataa agttttgtaa tataactact gtataaatta
      241 atcaagctat gtttatttga ttaaactatt aataaaaatc atatggtgaa tagatattat
      301 aataattaaa atacaaaaat agtagattcc aatttgtgaa aaaggagtat gtcgtttcat
      361 ttagaattta ttgcacgaaa ttagcatcga ataagggaaa ataagtgtta agttttaaat
      421 gataaaaaag attggaatgg atcgtcttga aatgctccct tcaaagtttt catttttcaa
      481 tgtctacttc gaagggggca tttccattaa attgttatag ctatttatat ttgtataatg
      541 aacatataag gttaagtagg tgcgagtgaa ggaaataaaa aagctcaaat gtactaaatt
      601 tttaatctta ataaatctct actttataaa gattgaatgg acattcgagc gttaatcagt
      661 caggagggaa tttcactcct acaatgtaat aataatactt gcttcaccac tatacaagga
      721 gtgagttgtt atgttcaaag tgaattattc gattttaagt tattatccag atatttatct
      781 ccatagtaat ttagctgtag gtgtgtcatt tgaaattata ggagaaagtt atcataagaa
      841 tgaaattaaa tttataaccc aaagaagaaa aatttcatca tttgatgatg aaatagacaa
      901 tttagtactt attaatatgt ttttggatgg acttaaatgt gaattcgaac aaactaatca
      961 atcaataaaa aattataaga aaaagtttgt taataatttt tactttgaga atattgaata
     1021 taaaatgttt gactcattaa aagaagttaa taattttatt gaaaaaacat
//
LOCUS       DAPIDM010000254         1070 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000254 DAPIDM010000000
VERSION     DAPIDM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.262"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..364
                     /locus_tag="RJE42_002736"
     CDS             <1..364
                     /locus_tag="RJE42_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878084.1"
                     /translation="DELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVETGKDTAT
                     LYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKGIYLKLKT
                     KKNAAWEYVLIGEKWGYQ"
     gene            375..551
                     /locus_tag="RJE42_002737"
     CDS             375..551
                     /locus_tag="RJE42_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878085.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
BASE COUNT          392 a          116 c          194 g          368 t
ORIGIN      
        1 tgatgaactt gagaaaaaag gttataaact ggaagatata tttacaaaag aagaaataaa
       61 aaaatataaa gctgaagacc aattgagagc gggtaaaact caatatgtag aaacaggtaa
      121 agatactgca acattatatc tttcttctgc atatacaaaa acaatagctg ctttaggtgc
      181 gggggctatt agcgttattt ctgcactaac aggaggttta gttggcgcag gtgttggtgg
      241 tttccttgga tctatagctg catcgaatat tgatactagt aagggaatat atttaaaatt
      301 aaaaactaaa aagaatgcag cctgggaata cgttctgata ggagaaaaat ggggatatca
      361 gtaagggtga ttttatggat agattaaaat attcacttaa agttggaatt ttagcattat
      421 tattattttg tactttaaat tatttagttc caatgcaaag caatgctttt tcaataatta
      481 tatattcggc aatttttgct gtgttactta tgcttttagt ttatatattt ttaggaattt
      541 taaagaaata acatgaaaca aattagcatt ggctatgaaa aatctatgag gatagaattt
      601 ttcatagcca ttttttaaaa gagcaagaag taaaaaattg gaaaaccgtc aatcaaataa
      661 ttgaaaaaga acacttggac aaaatgaata tgaagccgag ccaccgttgt tcttcttttc
      721 gggatcgttg gattagattc ggttgtacag tataatggaa aatattataa taaatttata
      781 aaaaaaggta ttttattttg aaatcatgtc cctccgttac atttagtagt ggaggagttt
      841 tattttaata atgtttacac ctatattttt aaatttatta aatgatgtag gttcaatttt
      901 ttagttaggg aggggaatca attgaaagtt attgagaaag ttcattatga agatttttta
      961 gaaccaaaaa tagttcaatt aattcagtca ttagttaagg acaaaatgga tcaacttttt
     1021 attacttctg aacattttgc tattgacata gacaaggtat tatttcaact
//
LOCUS       DAPIDM010000255         1049 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000255 DAPIDM010000000
VERSION     DAPIDM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.263"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..501)
                     /locus_tag="RJE42_002738"
     CDS             complement(<1..501)
                     /locus_tag="RJE42_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HDV5878086.1"
                     /translation="MHVPKYFQVDDFKEIEKFIQSNSFGMIVTVDQGKPIATHVPLVL
                     DKKGDDYYISGHLAKTNPQCNTLKDNQNVLIVYQGAHSYVSSAWYEKENVPTWNYQAV
                     HLYGESSILSEEETIDDLKSLLVKYERHREDPVLWDNISENTKMQAKGIVGFKVKIHK
                     IEAAYKM"
     gene            complement(635..1036)
                     /gene="msa"
                     /locus_tag="RJE42_002739"
     CDS             complement(635..1036)
                     /gene="msa"
                     /locus_tag="RJE42_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDV5878087.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVVPIMISGI
                     LFFKTNLDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEHIDVLITSKD
                     FGFDEILFYTLYLLLILIILYYLKKQVKTKS"
BASE COUNT          382 a          170 c          113 g          384 t
ORIGIN      
        1 cattttgtat gcagcctcta tcttgtgtat cttgacttta aatcctacaa tacctttggc
       61 ctgcattttg gtattttcag aaatattgtc ccataatact ggatcttcac gatgcctttc
      121 atactttacc aataggcttt tcaagtcatc tattgtttct tcctcactta atatacttga
      181 ttcaccatat aaatgaactg cttgataatt ccaagtaggt acattttctt tttcatacca
      241 cgctgaagaa acgtaagaat gtgcaccttg atatacgatt aatacatttt gattatcttt
      301 taatgtatta cattgaggat ttgttttagc tagatgccca ctaatataat agtcgtcccc
      361 ttttttatct aaaactaatg gtacatgcgt agcaataggc tttccttgat ccacagttac
      421 aatcattcca aaagaattac tttgaataaa cttttcaatt tctttaaaat catctacttg
      481 aaaatacttc ggcacatgca tatgatctcc ccctttgaat aacaatacat ttgaatgtat
      541 tttatcatta aaaaatctaa ggataaataa gttagagtaa tgtcatagta ttactcgttt
      601 ataaaataaa ctatgaaaca aaaataatta agctttaact tttagtttta acttgctttt
      661 ttaaataata taaaatgata aggattaata acaaatataa tgtataaaac agaatttcat
      721 caaatccaaa atctttactt gtaattaata catctatatg ttctaattcc atcatctctg
      781 ctttaatata attattaaac tttggcagag tcattaaatg tacattataa atataataat
      841 aaaagtctat gaatatgatg ttaaaaaata tgtatgtctt atctaaatta gttttaaaga
      901 ataatatccc agaaatcatt atcggaacca ctaatacaat catcattgct gctaaaatat
      961 taatatttaa accaattgca gataatacac cgaaaactaa taaattagca actaaactaa
     1021 ggataagata tttcataaaa tcacctcgt
//
LOCUS       DAPIDM010000256         1042 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000256 DAPIDM010000000
VERSION     DAPIDM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.264"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            317..>1042
                     /locus_tag="RJE42_002740"
     CDS             317..>1042
                     /locus_tag="RJE42_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDV5878088.1"
                     /translation="MIHSRKLRLCLYLVLLAVFIGACGMKKEESSKDKQIKENFTKTL
                     NLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIVDSEMVIELKDKKMESRSMVLYINRNT
                     RTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNSNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIAHKNLEFTFIENKE"
BASE COUNT          441 a          103 c          184 g          314 t
ORIGIN      
        1 taatagaaaa atatgatgga aatatgtttg ttagaaacta ttatgaaaat atgttgagta
       61 atataaatgt gtagttttag ataatagtat tgtttgtttt ttacctattc acataattat
      121 tattaaaatt aaatattaaa ataaaaaagc ttctgataag attttaggat tgaaaaacct
      181 atatcaaatt aggagctttt aatttatgag agtatcttaa tttataaaaa aattaaagta
      241 catagcataa atctgatatt atattataag caaaaataac ctgaagtgat aaatcactca
      301 agggggatgt gaaatgatga ttcattcaag aaagttgagg ctttgcctgt atttagtatt
      361 attagctgtt tttatagggg cttgcggaat gaaaaaagaa gaaagtagta aagataaaca
      421 aattaaagaa aacttcacca aaacgttgaa cctgtatcca actaaaaatc ttgaagactt
      481 ttatgataag gaaggatttc gagatcaaga gtttgaaaag ggcgacaaag gcacttggat
      541 tgttgattct gagatggtaa ttgagttgaa ggataaaaaa atggaatcca gaagtatggt
      601 gctctatata aatcgaaata ctagaacaac gaaaggtaat tttattgtta gagaattgtg
      661 ggaagacagt aaagggtatg cccaaagtaa agatacaaaa tatccagtaa aaatggaaca
      721 taataaaatt atcccaacca aaccgatagc tgatgataaa ttaagaaaag aaatcgaaaa
      781 ctttaagttt tttgtacaat acggtgactt taaagatatt aatgattata aagatggtga
      841 catttcatat aattcgaatg tgccaagtta ttcagcaaag tatcaattaa gtaatgatga
      901 ctacaatgtg aagcaactta gaaagaggta tgatatacca actaagaaag cacctaaatt
      961 attaataaaa ggcgatggtg atttaaaagg atcatctata gcacacaaaa atttagagtt
     1021 tacatttata gaaaataaag aa
//
LOCUS       DAPIDM010000257         1038 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000257 DAPIDM010000000
VERSION     DAPIDM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.265"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            183..297
                     /gene="rrf"
                     /locus_tag="RJE42_002741"
     rRNA            183..297
                     /gene="rrf"
                     /locus_tag="RJE42_002741"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..385
                     /locus_tag="RJE42_002742"
     tRNA            310..385
                     /locus_tag="RJE42_002742"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Val,seq:tac)
     gene            402..477
                     /locus_tag="RJE42_002743"
     tRNA            402..477
                     /locus_tag="RJE42_002743"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:435..437,aa:Thr,seq:tgt)
     gene            484..556
                     /locus_tag="RJE42_002744"
     tRNA            484..556
                     /locus_tag="RJE42_002744"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:517..519,aa:Lys,seq:ttt)
     gene            590..664
                     /locus_tag="RJE42_002745"
     tRNA            590..664
                     /locus_tag="RJE42_002745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Gly,seq:gcc)
     gene            672..760
                     /locus_tag="RJE42_002746"
     tRNA            672..760
                     /locus_tag="RJE42_002746"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:706..708,aa:Leu,seq:taa)
     gene            766..842
                     /locus_tag="RJE42_002747"
     tRNA            766..842
                     /locus_tag="RJE42_002747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:800..802,aa:Arg,seq:acg)
     gene            863..936
                     /locus_tag="RJE42_002748"
     tRNA            863..936
                     /locus_tag="RJE42_002748"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Pro,seq:tgg)
     gene            960..1035
                     /locus_tag="RJE42_002749"
     tRNA            960..1035
                     /locus_tag="RJE42_002749"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:993..995,aa:Ala,seq:tgc)
BASE COUNT          281 a          220 c          252 g          285 t
ORIGIN      
        1 tgaagaataa aaaagttgat taaaagtgtt gactttatca attgaatgaa gtaatatata
       61 aaagtcgtca aaaacagacg aaacacacta aaagctgatg tgacaaagtt cacaacaaag
      121 tgtaaaatta actattgcac cttattaatt aagcgtgtat cataaataag taagttattt
      181 tgtctggtga ctatagcaag gaggtcacac ctgttcccat gccgaacaca gaagttaagc
      241 tccttagcgt cgatggtagt cgaacttacg ttccgctaga gtagaacgtt gccaggcaaa
      301 tgacaaatcg gagaattagc tcagctggga gagcatctgc cttacaagca gagggtcggc
      361 ggttcgaacc cgtcattctc caccatttat tcttacatat agccggccta gctcaattgg
      421 tagagcaact gacttgtaat cagtaggttg ggggttcaag tcctctggcc ggcaccatct
      481 tttgagccat tagctcagct ggtagagcat ctgactttta atcagagggt cagaggttcg
      541 aatcctctat ggctcattac gatttaattt ttatatttag caaaataatg cagaagtagt
      601 tcagcggtag aatacaacct tgccaaggtt ggggtcgcgg gttcgaatcc cgtcttctgc
      661 tccattattt tgccggggtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggtg
      721 agagatcacc gtaccggttc gattccggtc ctcggcacca ttttagcgcc cgtagctcaa
      781 ttggatagag cgtttgacta cggatcaaga ggttatgggt tcgactccta tcgggcgcgc
      841 catttttaaa ttaattgaat aacgggaagt agctcagctt ggtagagcac ttggtttggg
      901 accaaggggt cgcaggttcg aatcctgtct tcccgattac ttcttaaatt ccattttatg
      961 ggggcttagc tcagctggga gagcgcctgc tttgcacgca ggaggtcagc ggttcgatcc
     1021 cgctagtctc caccattt
//
LOCUS       DAPIDM010000258          992 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000258 DAPIDM010000000
VERSION     DAPIDM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.266"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>992
                     /locus_tag="RJE42_002750"
     CDS             <1..>992
                     /locus_tag="RJE42_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDV5878089.1"
                     /translation="IIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTN
                     AIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILL
                     CYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFI
                     DGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDI
                     DTMLLKLAKQYHANIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLT
                     KMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIE"
BASE COUNT          347 a          136 c          167 g          342 t
ORIGIN      
        1 attattcctg aaattgcaaa tgatcttgga ttacaaaact ccagcttttt aaaaaatcac
       61 tatgtagatg gcattatcgg tagtattttt atgttcttaa tttttggtgt atttattaga
      121 cgagttacta acgctataaa aggtttagaa cattttatta tgcgtagaag tgctgttgaa
      181 atactattcg caacaatagg tttaataatc ggattactta tttctgttat ggtgtcgttt
      241 atattggaat caattggcaa ctctattttt aatcatttca ttcctgtcat aattacgata
      301 ttactatgtt atttcggttt ccaatttggc cttaaaaaac gagatgaaat gttaatgttt
      361 ttacctgaga atatagcgcg ttccatgtca caacatacta aaagtgctac accaaaaatt
      421 atcgacacaa gcgcaattat tgatggtcgt attttagaag tcattcgttg cggttttatc
      481 gatggcaata ttttaattcc acaaggtgtt attaatgaat tacaaattgt tgcagattca
      541 aatgacagtg ttaaacgtga aaagggtaaa agaggcttag atattttaaa tgaattgtat
      601 gatttagatt atcctacaaa agtcatacat ccaactaaaa cacatagtga tattgataca
      661 atgctattaa aactagcaaa acaatatcac gcaaatatta taacgacaga ctttaatttg
      721 aataaagttt gtcatgtaca tggtatcaaa gcattaaatg ttaatgattt atcagaagca
      781 atcaaaccta atgtacatca aggtgatcaa cttcatattt tactgacaaa aatgggtaaa
      841 gagcctggtc aagcagtagg atatctagat gatggtacga tggtagttgt tgataatgct
      901 aaaaatctta ttggcagtca tgtcaattta gaagtagtca gcttattgca aacatcttca
      961 ggaagaattg tttttgctaa aaaaatcgaa ga
//
LOCUS       DAPIDM010000259          974 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000259 DAPIDM010000000
VERSION     DAPIDM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.267"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>974
                     /gene="femB"
                     /locus_tag="RJE42_002751"
     CDS             <1..>974
                     /gene="femB"
                     /locus_tag="RJE42_002751"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDV5878090.1"
                     /translation="FSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKR"
BASE COUNT          342 a          129 c          187 g          316 t
ORIGIN      
        1 ttttctctaa aattcctact atgggaagtt atgtttacta ttcgaatcgt ggtccagtaa
       61 tggatttttc agatttagga ttagttgatt attatttaaa agagttagat aaatatttac
      121 agcaacatca atgtttatat gttaaattag atccgtattg gttatatcat ctatatgata
      181 aagatatcgt gccatttgaa ggtcgcgaga aaaatgatgc cttagtaaac ttgtttaaat
      241 cgcatggtta cgagcatcat ggctttacaa ctgagtatga tacatcaagc caagtacgat
      301 ggatgggcgt attaaacctt gaaggtaaaa cacctgaaac attgaaaaag acatttgata
      361 gtcaacgtaa acgtaatatt aataaagcga taaactatgg tgttaaagtc agattccttg
      421 aacgtgatga gttcaatctt ttcctagatt tatatcgcga aactgaagag cgtgctggat
      481 ttgtttcaaa aacagatgat tatttttata actttattga cacatatgga gataaagtat
      541 tagtaccatt agcatatatt gaccttgatg aatatgtgtt aaagttgcaa caggaattga
      601 atgacaaaga aaatcgtcgt gatcaaatga tggcgaaaga aaacaaatca gataaacaaa
      661 tgaagaaaat tgcagaatta gataagcaaa ttgatcatga tcagcatgaa ttattgaatg
      721 caagtgaatt gagcaaaacg gacggcccaa ttctaaacct tgcttctggc gtttattttg
      781 caaatgcata tgaagtgaat tatttctctg ggggttcatc agaaaagtat aatcaattta
      841 tgggaccata catgatgcat tggtttatga ttaactattg cttcgataat ggttatgatc
      901 gttataattt ctatggttta tcaggtgatt tcacagaaaa cagtgaagat tatggcgtat
      961 accgctttaa acgt
//
LOCUS       DAPIDM010000260          943 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000260 DAPIDM010000000
VERSION     DAPIDM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.268"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            88..294
                     /locus_tag="RJE42_002752"
     CDS             88..294
                     /locus_tag="RJE42_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDV5878091.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            291..494
                     /locus_tag="RJE42_002753"
     CDS             291..494
                     /locus_tag="RJE42_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878092.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            487..723
                     /locus_tag="RJE42_002754"
     CDS             487..723
                     /locus_tag="RJE42_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878093.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKTPEEKAKIENDFIKDINDKDS
                     EFYSPMMANMNEHELRAMLRMMPSLIDTGDDNDD"
     gene            713..>943
                     /locus_tag="RJE42_002755"
     CDS             713..>943
                     /locus_tag="RJE42_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878094.1"
                     /translation="MMIKKLKNMDWFDIFIAGILRLFGVIALMFVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIE"
BASE COUNT          386 a          116 c          192 g          249 t
ORIGIN      
        1 aatcatgaaa gacaagatgg aacagcagac gcaggaaaag gatacgtgta aagacatctt
       61 agatcgagtc aaggaggttt tggggaagtg acacaatact tagtcacaac attcaaagat
      121 tcaacaggac gtaaacatac acacataact aaagctaaga gtaatcaaag gtttacagtt
      181 gttgaggcag agagtaaaga agaagcgaaa gagaagtacg agaaacaagt taaaagggat
      241 gcagttatta aagtgggtca gttgtttgaa aatataaggg agtgtgggaa atgacggagg
      301 ttaaaattaa aactatttca gatagagttt attacacaac aacagatcta gcttctgacg
      361 attatattaa tcttgttatg aatctagtga ttgaggattt tcttccggtc aaagatgtgt
      421 tcaacaatga agtatgggtt aaaagagatg agattgaatc atttacattt attaaggagg
      481 caaacgatga ttaacatacc taaaatgaaa ttcccgaaaa agtacactga aataatcaaa
      541 aaatataaaa ataaaacacc tgaagaaaaa gctaagattg aaaatgattt tattaaagat
      601 attaatgata aagacagtga attttacagt cctatgatgg ctaatatgaa tgaacatgaa
      661 ctaagggcta tgttaagaat gatgcctagt ttaattgata ctggagatga caatgatgat
      721 taaaaaactt aaaaatatgg attggttcga tatctttatt gctggaatac tgcgattatt
      781 cggcgtaatc gcactgatgt ttgttgtcat atcgcctatc tatacagtgg ctagttacca
      841 aaacaaagaa gtacatcaag ggacaattac agataaatat aacaagagac aagataaaga
      901 agacaagttc tatattgtat tagacaacaa acaagtcatt gaa
//
LOCUS       DAPIDM010000261          919 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000261 DAPIDM010000000
VERSION     DAPIDM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.269"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            34..432
                     /locus_tag="RJE42_002756"
     CDS             34..432
                     /locus_tag="RJE42_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878095.1"
                     /translation="MLPFSQDSSGDLDLKEVWKKNRLEMISECGMALFLFGNKEKDGK
                     IVLADGLEEEYKIAERQELVRLPINVTGYKTKNLSEQYNEEINIQFKEKILKMYNEIN
                     EYKCDFSNKQSIDELVQKIVNLVIEIKKTK"
     gene            526..>919
                     /locus_tag="RJE42_002757"
     CDS             526..>919
                     /locus_tag="RJE42_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDV5878096.1"
                     /translation="MKYPIRQVAEVLPEKYTRYILLKEFQRLFPYQWNIIVERQQTYK
                     EKAQHLYKVKKIKNRYNTKSAEEYFFSIPQVKYILSAGRMKKHKENYNASEIKIKKAA
                     LEKSRKNKNWKIEERLIKAKRYTQKVDPE"
BASE COUNT          428 a           88 c          156 g          247 t
ORIGIN      
        1 aataagaagt ctaaactaca gaatcattta aatatattgc cattttctca agattcaagt
       61 ggcgatttag acttaaaaga agtatggaaa aaaaatcgct tggaaatgat ttctgagtgt
      121 ggaatggcat tatttttatt tggaaataaa gaaaaggatg gaaaaattgt tttagctgat
      181 ggattagaag aagaatataa aatagcagaa agacaagaat tggttagact ccctataaat
      241 gtgacaggtt ataaaacaaa gaatctttca gaacaatata acgaagagat aaatatacaa
      301 tttaaagaga aaatattaaa aatgtataac gaaataaatg aatacaaatg tgatttttca
      361 aataaacaaa gtattgatga attagttcaa aaaatagtaa atttagtaat tgaaataaaa
      421 aaaacaaaat aattcatggg gttttaatgc tttagaaaga taggtgagta atttgggaaa
      481 gtaaagagaa aacatgatca cgaagaagtt tattttaata aaagcttgaa gtaccctata
      541 cgacaagtag cagaagtttt gccagagaaa tatacaaggt atattttatt aaaagaattt
      601 cagcgattat ttccatacca gtggaatata attgtggaaa gacaacaaac ttacaaagaa
      661 aaagcgcaac acctatataa agttaaaaaa atcaaaaata gatataatac gaaaagtgca
      721 gaagaatatt ttttctcgat tcctcaagta aaatacattc tttcagcagg aagaatgaaa
      781 aaacataaag agaattataa tgcttcagag ataaaaataa aaaaagcagc tttagaaaag
      841 agtaggaaga ataagaattg gaaaattgaa gaacgtttaa taaaagctaa gagatatact
      901 caaaaagttg atccagaat
//
LOCUS       DAPIDM010000262          914 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000262 DAPIDM010000000
VERSION     DAPIDM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.270"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..893
                     /locus_tag="RJE42_002758"
     CDS             <1..893
                     /locus_tag="RJE42_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV5878097.1"
                     /translation="NETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIPVVAV
                     LHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPVINI
                     PVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIRRLVSYVPKIELHMYGF
                     GSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALLESLAH
                     GVPVISYDIKYGPGELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAK
                     AQQYSKASLIKQWDQFVRLI"
BASE COUNT          317 a          145 c          149 g          303 t
ORIGIN      
        1 ataatgaaac ggaattaggc gcatttttca ttaaacaact atatcaacat ggggatttat
       61 ttttcagtga ccgtaatgtg tatacagcac ctattttcaa cttaacacct gagtcaatcc
      121 cagtagtagc tgtgcttcac agtacacaca ttaaaaatat tgatgcatta gattcatcgc
      181 catttaaaaa tgtctacaaa gcgatgtttg aaaatttatc tcgatatcgt gcaattatcg
      241 tttctactga gcagcaaaaa ttagatgttg aaaaacgtat taaccatact attccagtta
      301 ttaatattcc tgtcggctat agtgaaacaa ttgatacacc agtccaaaca ttagatcagc
      361 gcagtgttaa attaatttcc gttgctcgtt attcacctga aaaacaattg catcaacaaa
      421 ttgaattaat tagacgctta gtatcttatg tcccaaaaat agagcttcat atgtatggtt
      481 ttggttctga aagtaaaaaa ttaaatgaat tgatacaaaa atatggattg gaaaatcatg
      541 tttacttacg cggcttttta agcaatctag accaagaata tagcgatgcc tatttgagtt
      601 taataacgag taacatggaa ggtttctcgt tagcgctact tgaatcttta gcacatggtg
      661 ttccagttat tagttatgat attaaatatg gtcctggtga attaatcact tcagatttca
      721 atggttattt aattactaaa aatgatgaag atgccctatt tgataaagta aaatatgtga
      781 ttgatcatcc tgaagtacaa caacgactgt caaaaggtag tttggcaaaa gcgcaacaat
      841 atagtaaagc gtcgttaatt aaacaatggg accaatttgt tcgtctaata taaatgtact
      901 ttacctttat tttt
//
LOCUS       DAPIDM010000263          876 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000263 DAPIDM010000000
VERSION     DAPIDM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.271"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>876
                     /locus_tag="RJE42_002759"
     CDS             <1..>876
                     /locus_tag="RJE42_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDV5878098.1"
                     /translation="MAGNQRYVFLDHNNQFVADQNAKHTYSNVVRSIHSSLFTENTII
                     NYLVELTACWTIFMILSGIYLLIKKHLISNKSKALHWQKWHAMIGIIIAIPVFVLVLT
                     GLPWSGFMGSKIAGMMDTNSDLGQGELAINPPKSDLNELPWATRKNKQPASSEKGSSG
                     HHGKSAMPQTKLDYQISIDKVVEQAQKSGIKKPFSIVYPSDKHGTFIVSNTSNSGVTG
                     LDVSPYKEQTLYFDQYSGKKLGTIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLF
                     VCIALLVAIGMGFVS"
BASE COUNT          283 a          136 c          165 g          292 t
ORIGIN      
        1 tatggcagga aatcagcgtt acgtctttct agatcataac aatcaatttg ttgcagatca
       61 aaatgcgaaa catacgtatt ctaatgtggt gcgaagtata catagttctt tgtttactga
      121 aaatactatt attaattatt tagttgagtt aaccgcatgt tggacgatat tcatgatttt
      181 atctggtatt tatttactca ttaagaagca tttaatttct aacaaaagta aggcacttca
      241 ttggcaaaag tggcacgcaa tgattggaat tatcattgca attccagtat ttgtattagt
      301 tttaactgga ttgccatggt ctggttttat gggcagtaaa attgccggta tgatggacac
      361 aaacagtgat cttggtcaag gtgaattagc gattaatcca cctaaatcag atttgaatga
      421 gttaccttgg gctacacgta aaaataaaca gccagcttca tccgaaaaag gttcaagtgg
      481 tcatcatggt aaatcagcaa tgcctcaaac caaattagat tatcaaatat ctattgataa
      541 ggtcgttgaa caggcgcaaa aatctggtat taaaaagccg ttttcaatcg tatatccaag
      601 tgataaacat ggtaccttta ttgtatcaaa tactagtaat tcaggtgtta ctgggctaga
      661 tgtatcacct tacaaggaac aaacacttta tttcgatcaa tatagcggta aaaaactagg
      721 tacgattaaa tatgatgact acggtattat tgctaaatgg tttacatggg gcattccgct
      781 tcacgaaggt catttattcg gtattttaaa caaaatcatt aatttatttg tatgtatcgc
      841 tttattagta gccattggca tggggtttgt ctcttg
//
LOCUS       DAPIDM010000264          864 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000264 DAPIDM010000000
VERSION     DAPIDM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.272"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>864
                     /gene="guaC"
                     /locus_tag="RJE42_002760"
     CDS             <1..>864
                     /gene="guaC"
                     /locus_tag="RJE42_002760"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV5878099.1"
                     /translation="ECDTTIQFGPKKFKLPVVPANMQTVMNEKLAKWFAENDYFYIMH
                     RFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIEKLAQEKLIPEYITIDIAHGHSD
                     SVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAGADATKVGIGPGRVCITKIKTGF
                     GTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKSIRFGASMVMIGSLFAAHEESPG
                     ETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVEHKGSLMDTLKEMQQDLQSSISY
                     AGGKDLKSLRTV"
BASE COUNT          304 a          120 c          180 g          260 t
ORIGIN      
        1 tgaatgtgat acaactatcc aatttggtcc gaaaaaattc aagctacctg tagttcctgc
       61 aaatatgcaa acagttatga atgagaaatt agcgaaatgg tttgctgaaa atgattactt
      121 ttatatcatg catcgttttg atgaagaagc aagaatacct tttataaaac atatgcaaaa
      181 ttcaggctta tttgcatcta tttcagttgg tgtaaagaaa gcggaatttg attttataga
      241 aaaattagct caagaaaagt taatccccga atatattaca atagatattg cgcatggtca
      301 ctcagattca gtgataaaca tgattaaaca tattaaaacc catatacctg atagttttgt
      361 tattgctggt aatgttggta cgccagaagg tgttagagaa ttagaaaatg ctggtgctga
      421 tgctaccaaa gtcggtatag gtcctggtag agtttgtatt acaaagatta aaacaggttt
      481 tggtactggt ggttggcagt tagcggcatt aaacatatgt agtaaagcag ctcgtaaacc
      541 tttgattgcc gatggtggta taagaacgca tggcgacatc gctaagtcaa ttagatttgg
      601 tgcatcaatg gtcatgattg gttcattatt tgcggcacac gaagaatcac ctggtgaaac
      661 tgtagaactt gatggtaaac agtataaaga atattttggt agtgcatctg aatttcaaaa
      721 aggcgaacat aaaaatgtag aaggtaaaaa aatgtttgta gaacataagg gttcattaat
      781 ggatacctta aaagaaatgc aacaagattt acaaagctca atttcatatg ccggtggaaa
      841 agacttgaaa tcattacgta ctgt
//
LOCUS       DAPIDM010000265          837 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000265 DAPIDM010000000
VERSION     DAPIDM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.273"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>837
                     /locus_tag="RJE42_002761"
     rRNA            <1..>837
                     /locus_tag="RJE42_002761"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          227 a          185 c          248 g          177 t
ORIGIN      
        1 aagacattgt gtcttcgagt cgttgatttc acactgccga gaaaagcctc tagatagaaa
       61 ataggtgccc gtaccgcaaa ccgacacagg tagtcaagat gagaattcta aggtgagcga
      121 gcgaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga aggggtgctc
      181 tttagggtta acgcccagaa gagccgcagt gaataggccc aagcgactgt ttatcaaaaa
      241 cacaggtctc tgctaaaccg taaggtgatg tataggggct gacgcctgcc cggtgctgga
      301 aggttaagag gagtggttag cttctgcgaa gctacgaatc gaagccccag taaacggcgg
      361 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac
      421 gaaaggcgta acgatttggg cactgtctca acgagagact cggtgaaatc atagtacctg
      481 tgaagatgca ggttacccgc gacaggacgg aaagaccccg tggagcttta ctgtagcctg
      541 atattgaaat tcggcacagc ttgtacagga taggtaggag cctttgaaac gtgagcgcta
      601 gcttacgtgg aggcgctggt gggatactac cctagctgtg ttggctttct aacccgcacc
      661 acttatcgtg gtgggagaca gtgtcaggcg ggcagtttga ctggggcggt cgcctcctaa
      721 aaggtaacgg aggcgctcaa aggttccctc agaatggttg gaaatcattc atagagtgta
      781 aaggcataag ggagcttgac tgcgagacct acaagtcgag cagggtcgaa agacgga
//
LOCUS       DAPIDM010000266          832 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000266 DAPIDM010000000
VERSION     DAPIDM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.274"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(286..396)
                     /locus_tag="RJE42_002762"
     CDS             complement(286..396)
                     /locus_tag="RJE42_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878100.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
BASE COUNT          301 a          134 c           63 g          334 t
ORIGIN      
        1 tagctatcat cacttcataa tagttaaatt tcatattttc aatttttgtt tctacatatt
       61 aacaactttt catcatcaat tactttgtca aagacaaacg atttgtcttg tataccttac
      121 gccttttact aaacttcaat gtttaacctt ttatgtattt ttattagatt agtgtctttc
      181 aatattcaaa ctactaactc cagtagtcta ttaaatttga gatatagaga tatctttaga
      241 ctttaatttt tccggagtca tatagacttc tcaaatacta tacatttatt tcataatttt
      301 gaaccctaat attactaaaa taattataaa cgtccctatt aaaaattcta ttttctcttt
      361 tttatttgtt tttcgcactt taatagtcat cttcatttta ctaatggtga gaaagtaata
      421 ttttattcaa agaatatgac ttcgtattaa atcaaacctc acattcttat tagtttgcaa
      481 attcaatagt cctatcagaa ttaaaatcaa aacattcaaa ctattttatc gtttttactt
      541 cctcgaccat tttcacctcc ttataatcta tataaattat gtttaccaca cgattcttta
      601 cactcgatga attaattttg ttaagaaaca tttaggcatg taactttgta ataaaaacat
      661 tttcaaataa atgtaccaaa aactttcatg aaataaagta tacttttcaa atctattaat
      721 tttagaatca taaaaccaaa tgctatatta caatataatt atcacaaatc atcatatatg
      781 ccaattacac aatttaatat aaaaaattgt caatgaaaaa ttatgcacat aa
//
LOCUS       DAPIDM010000267          810 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000267 DAPIDM010000000
VERSION     DAPIDM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.275"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>810
                     /locus_tag="RJE42_002763"
     CDS             <1..>810
                     /locus_tag="RJE42_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSau1 family transposase"
                     /protein_id="HDV5878101.1"
                     /translation="SRNNLNQSYQAETAQKNYETKRKLCGRPTRFTPELGNIIKYYLK
                     CHWSPEQIVGRLLQNQICFKTIYRWINSNMINFELISCLRQKGKRQKPKETRGKFNIG
                     RPISQRPKEVKKRNTFGHWEADTIVSSRGKSKGCIATFAERKSRYYYCVLMPDRSSNS
                     METAINNLIKHLPKGAVKTITVDRGKEFSCYQNIENQFNINVYFADPYSAWQRGTNEN
                     TNGLLREFFPKKTDLAKVNQEQLNYALDSINYRPRKCLNWKFPYEVLCDELL"
BASE COUNT          297 a          138 c          134 g          241 t
ORIGIN      
        1 agtaggaata atttaaatca gtcgtatcaa gctgaaacag ctcaaaaaaa ttatgaaacc
       61 aaaagaaaac tttgtggtcg tcctactcga tttactccag aacttggtaa tatcattaaa
      121 tattatttaa aatgccactg gtctcctgaa caaatcgtag gtcgtctatt acaaaatcaa
      181 atctgtttta aaaccatcta tcgatggatt aactctaata tgattaattt cgaacttatt
      241 tcatgccttc gtcaaaaagg aaaaagacag aaacctaaag aaactagagg taagtttaat
      301 attggccgtc ctatttcaca acgtcctaag gaagtaaaga aacgtaatac attcggtcat
      361 tgggaagctg acacaattgt atccagcaga ggcaaaagta aagggtgcat tgctaccttc
      421 gcagaaagaa aatcacgtta ttattattgt gtacttatgc ctgatcgttc ctctaattct
      481 atggagacgg caattaataa ccttatcaaa catctaccta aaggtgccgt taaaactatt
      541 actgtcgata gagggaaaga gttctcttgt tatcaaaata ttgaaaacca atttaatatt
      601 aatgtctact tcgctgatcc atattccgca tggcaaagag gtacaaatga aaataccaat
      661 ggtttactaa gagaattttt ccctaagaaa acagacttag ctaaagtaaa tcaagagcaa
      721 cttaactatg cattagactc aattaattat cgaccaagaa agtgtttaaa ttggaagttt
      781 ccttatgaag ttttatgtga tgaactgttg
//
LOCUS       DAPIDM010000268          808 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000268 DAPIDM010000000
VERSION     DAPIDM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.276"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..348
                     /locus_tag="RJE42_002764"
     CDS             <1..348
                     /locus_tag="RJE42_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDV5878102.1"
                     /translation="RDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFIYELRGDGNINF
                     NVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEYKKEQEREKEKE
                     KQKDDDHSSLDEV"
BASE COUNT          350 a          107 c          114 g          237 t
ORIGIN      
        1 agagatggtt atcttttaag tgataaaaaa tataaagatt ggacagaaca taaccaagat
       61 caaattaaaa aaggcaaaac tgcacaagcc atgtttatct atgagttaag aggtgatgga
      121 aatattaatt ttaatgtcca taaatactca gaagataaaa cagttgattc taaatcattc
      181 aaatttagta aacttaaaac cgaagatttt tctcatagag cggaaacaag agaagaagta
      241 gaaaagaaag aaaaagaatt tgaagaagag tacaaaaaag aacaagaacg agagaaagaa
      301 aaagaaaagc aaaaagatga cgaccacagt agtttagatg aagtataaat ttatgaatgc
      361 tcgcatttag cgttataatc tttgtaatta ttattgtcat tgtaaattag cttacttaca
      421 ctcataatga cacactaatt taattgttaa atataacaaa aaagagtagc tcgcctactc
      481 ttttttgttg ttttgaaatg gtcatttttg aaatgactcg atcataattt cacgaatacc
      541 atgcaacatt tttcgtgaac tcatttttca aacaatactt aatttgttac tatttcactt
      601 ctttttatta ttagatgaaa acaaaaatgc tccaaaaaag agtaagggaa aaataacaaa
      661 caaacgagca tcaaatgtat ttttcccaag aattacaggt aaagatatat aaataataaa
      721 agttattatt aaaaacaaca aataaacttt tttattggta ctcatcacaa acacacacct
      781 ataatacaat agcaatgaaa gctcttgc
//
LOCUS       DAPIDM010000269          791 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000269 DAPIDM010000000
VERSION     DAPIDM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.277"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(75..584)
                     /locus_tag="RJE42_002765"
     CDS             complement(75..584)
                     /locus_tag="RJE42_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDV5878103.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT          266 a          142 c           91 g          292 t
ORIGIN      
        1 ttattacatt caataaaaat gaccattttc ttattaattc aaacatacta cgattaacaa
       61 tactattatg ttacttaagc taatttaaac gtttcaataa ctttccattt atcttcgtca
      121 tcgtcaaaac gtaacaaagt taattcatcg ataatttctt tatggtctac cccagctaat
      181 gctacttgac caaaaatatc ttcgaattct tggctagata gtccttgtgc tattgtaaag
      241 tgtggcacaa aaacatgttc agcttctcca tagaaatctt ctccattaaa gcgattaaac
      301 aattcttcta agtcgtccgt ctttgcaact ttaaagtaaa tcacattgtt cgttggtttg
      361 aagctagaag cttttgtagc atgaacttct actgctggta taccattaat acgagctcta
      421 acctgttcaa tgacagaatc taaatcacca tcttcaattt caaatggtgc tttaattgtc
      481 acatgtggtt taattcgtga atactgttta tcatatcttt tacggtaaga atccaccgct
      541 tcttgaaatg actttgatgg aattaatgct aatcctaaaa tcatctgaat tcctcctttg
      601 tttaacctat tgctattata tcaaatttct ttgaaaaccg ttacattaaa aataattcat
      661 tatacatctt ttgcaatttc gacaaattta aattaaatta aacagtaaag tcttttataa
      721 attacattat agactagact atatgaagac aattcatact aaatctacta atgtaaatta
      781 ttcataaagt t
//
LOCUS       DAPIDM010000270          786 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000270 DAPIDM010000000
VERSION     DAPIDM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.278"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT          284 a           93 c          107 g          302 t
ORIGIN      
        1 attagtataa atttatcatt cattctacac tattttaatg gaaatagcat gaaatgttca
       61 tcaatatata atagctattg tcatatacgt atcaagaaaa agattttctg actcgttgac
      121 cttgttcttt tttgttctaa aataaaacgt gaaaataaaa aattatctgc tttttatttg
      181 ttcgactagc aactattgtt ttttattcat ttacatcgat atgagttttt ggatatttta
      241 attcatttat taataatttt acgtgctcgt cgtcaagact atttaagtca agtttagcca
      301 tgactgttcc tcctttaacg ttgtataggc attaattttt aatactttct tatttgaaaa
      361 aagcaaaaaa agataggtga gatgcatata caacaaaaag tgtatattat caatttagca
      421 tctcgcaata taattttata caatgtataa taagatttaa agatgcagct catcaacatt
      481 ttcataaggg atttttaaaa ttaagtattg aaaaataaaa tatttatatt ataatataaa
      541 taagtcaacg accatgcgtg gacagtataa cgaagtacat gcgaatgtag tagccgttta
      601 ctatgagact aggcattata attgaatatc atagtataaa ttgccatgag agtagaagct
      661 tcagtttatt aagacttcga aatcatggtt ttttattttt aggatgaact ttttgttcat
      721 agtaaatata tatatatgtt tttaattcgg ctaaatcata acatttaaat ttttagatga
      781 agcaaa
//
LOCUS       DAPIDM010000271          740 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000271 DAPIDM010000000
VERSION     DAPIDM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.279"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            225..650
                     /locus_tag="RJE42_002766"
     CDS             225..650
                     /locus_tag="RJE42_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDV5878104.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT          306 a          109 c          103 g          222 t
ORIGIN      
        1 ataagatcga ctatatgctt ttcatgctta ttatatacaa ctaatgaatc cctataaact
       61 aaaaacattg cttaccactt ttcaaaatac acgagtcata cccctcgtta aaaactatta
      121 ctaaaaatca aaaatataaa aaaatcaaaa agtatgtttt aaactttatg aaacgtgtta
      181 tataaatagt gtaatatacc acaactttaa aaggagattg agatatgaaa ctaaaatcat
      241 ttgttactgc cactttagca ttgggattat tatcaacggt cggagctgca ttacctagtc
      301 acgaagcatc tgcagatagt aataacggct ataaagaaat gacaatggat ggttatcaca
      361 ctgttcctta cacgatttca gtagatggta ttactgcatt acatcgaact tactttgttt
      421 ttccagaaaa taaaaaggta ctttatcaag aaattgacag taaagtaaaa aatgaattag
      481 cttctcaacg tggtgttaca acagaaaaaa ttaataatgc acaaacagca acttatacac
      541 ttactttgaa tgatggtaat aaaaaagtag tgaatctaaa gaaaaatgac gacgcgaaaa
      601 attcaattga tccaagtaca atcaaacaaa tacaaattgt agttaaataa taattttaaa
      661 atgaaaatag ttatacaagg caaacgctga aaaattgcgt ttgtcttttt agtatgaaat
      721 gaataatcga acttgtaatt
//
LOCUS       DAPIDM010000272          728 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000272 DAPIDM010000000
VERSION     DAPIDM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.280"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..570
                     /locus_tag="RJE42_002767"
     CDS             <1..570
                     /locus_tag="RJE42_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDV5878105.1"
                     /translation="HNVAAHSFKVTKIAQYLATVEEYHGRKINWKSLYEKALNHDFAE
                     VFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQYRDVYKQRLQEGKDDSLE
                     GQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETIIQFDHLASVQDFINNIIP
                     EMLTENFIPRTELRETTMNILNKRKEENE"
     gene            567..>728
                     /locus_tag="RJE42_002768"
     CDS             567..>728
                     /locus_tag="RJE42_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2198 family protein"
                     /protein_id="HDV5878106.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFID"
BASE COUNT          281 a           89 c          127 g          231 t
ORIGIN      
        1 cacaatgttg cagcgcactc ctttaaagta actaaaattg ctcaatatct agcaacagtt
       61 gaagaatatc atggacgaaa gattaattgg aaaagcttat atgaaaaagc attaaatcat
      121 gatttcgctg aagtgtttac tggtgatata aaaacacctg ttaaatatgc gagtagtgaa
      181 ttaaaaaaat tattttcgca agttgaagaa gaaatggtag agacctttat tgaagaagaa
      241 attccattac aatatagaga tgtttataag caacgactgc aagaaggtaa agatgattca
      301 ttagaaggcc aaatactttc agttgctgat aaaattgatt tgctttatga aacatttgga
      361 gaaatacaaa aacgtaatcc cgaagaatta tttttcgaaa tttatgaaat gagtctagaa
      421 acaattattc aatttgacca tttagcatct gtacaagatt ttattaataa tatcattcca
      481 gaaatgttga ctgaaaactt tatacctaga acagaattaa gggaaacaac catgaacatt
      541 ttaaataaaa gaaaagagga aaatgaatga tatggtattt tagtgcagca ttctttccat
      601 gtgtcttggt agtattgttt agtgtaataa caagaagtaa atgggtcggt actattctga
      661 cattaatttt aattggtgcc tcaatctata aagagtattt ccataacgag tggattattt
      721 ttattgat
//
LOCUS       DAPIDM010000273          721 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000273 DAPIDM010000000
VERSION     DAPIDM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.281"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>721
                     /locus_tag="RJE42_002769"
     CDS             <1..>721
                     /locus_tag="RJE42_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISSau3 family transposase"
                     /protein_id="HDV5878107.1"
                     /translation="VEANANRYTFVWKKSIQNHESKLNENSKALYRDLVEEKIIPEIK
                     EDGDSDLTIEEIDLIGSHLDKEIEDLNHSIQNKDCTQIRKQTRKKRTEIKKFKKKFED
                     YSERKSKYEEQKSILKDRNSFSKTDHDATFMRMKEDHMKNGQLKPGYNLQIATNSQFV
                     LSYDLFQNPTDTRTLIPFLTMIQNTFGYLPEYIVADAGYGSEQNYMAIIDDFNKTPLI
                     TYGMFIKDKTRKFKSDIFNTQ"
BASE COUNT          309 a           89 c          119 g          204 t
ORIGIN      
        1 aagtagaagc taatgccaat aggtatacat ttgtgtggaa gaaaagtatt caaaatcacg
       61 aatcgaaatt gaacgaaaat tcaaaagcat tatatcgtga cttagttgaa gaaaaaataa
      121 taccagagat aaaagaagat ggagatagcg atttaacaat agaagaaata gatttaattg
      181 gtagtcattt agataaagaa atcgaagatt taaatcattc tattcagaac aaagattgta
      241 ctcaaattag aaaacagacc cgtaaaaaaa gaactgagat taagaagttc aaaaagaaat
      301 ttgaagatta ttccgaaaga aaaagtaaat atgaagaaca aaaatcgatt cttaaagata
      361 gaaatagctt ttctaaaact gatcatgatg caacttttat gagaatgaag gaagaccata
      421 tgaaaaatgg ccaacttaag ccaggataca atttacaaat agcgacaaat tctcaatttg
      481 ttttatctta tgacctattt caaaacccga cagatactag aactttaatt ccatttttaa
      541 caatgattca aaataccttc ggttatttac cggagtatat tgtagctgat gcaggttatg
      601 gtagtgagca aaactatatg gctattatag atgattttaa taaaacgcca cttattacgt
      661 atggtatgtt tattaaggat aaaacgagaa agtttaaaag tgacattttt aacactcaaa
      721 a
//
LOCUS       DAPIDM010000274          715 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000274 DAPIDM010000000
VERSION     DAPIDM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.282"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..450
                     /locus_tag="RJE42_002770"
     CDS             <1..450
                     /locus_tag="RJE42_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5878108.1"
                     /translation="DEFICPNNKRIGFKRYAYRNDRYGFKRDFKLYECDDCSACSLRQ
                     QCMKPNSKSNKKIMKNYNWEYFKAQINQKLSEPETKKIYSQRKIDVEPVFGFMKAILG
                     FTRMSVRGINKVKRELGFVLMALNIRKIVARRAGVFMSKRRLYVKLD"
     gene            complement(469..715)
                     /locus_tag="RJE42_002771"
                     /pseudo
     CDS             complement(469..715)
                     /locus_tag="RJE42_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          256 a           90 c          127 g          242 t
ORIGIN      
        1 gatgaattta tatgtcctaa taacaaaaga ataggtttta aaagatatgc ataccgtaat
       61 gatagatatg gttttaaacg tgacttcaaa ctatatgaat gcgatgactg ttcagcatgt
      121 tctttgaggc aacaatgcat gaagccaaat tcgaaatcca ataagaaaat catgaagaat
      181 tataattggg aatactttaa agcccaaatt aatcaaaagc tttctgaacc agaaacgaaa
      241 aaaatctata gtcaaagaaa aattgatgta gagcctgttt ttggatttat gaaggctatt
      301 ttgggtttca ctcgaatgtc agttcgagga ataaataaag ttaaacgaga gctaggtttt
      361 gtattaatgg cacttaatat aaggaaaata gtagctcgac gagctggtgt ttttatgtca
      421 aaaagaagac tctatgtcaa attggactga tgcgttcaat atcggaagtt aggcaactaa
      481 acattgctta acttcctttt actttttgga gcgtaaagtt ttgaacataa taatattcga
      541 ttgcgcaaat gattgtaatt tccataacta aaagatgtac gtttaattaa ttttattttg
      601 ttatttatac cttctaaagg accatttgat aaattgtaat aatcaaatgt attttttata
      661 aagtatgcat gtttgttaag tgtcttaatt gctcgttgga caactaaatg cgttt
//
LOCUS       DAPIDM010000275          694 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000275 DAPIDM010000000
VERSION     DAPIDM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.283"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(158..>694)
                     /locus_tag="RJE42_002772"
     CDS             complement(158..>694)
                     /locus_tag="RJE42_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HDV5878109.1"
                     /translation="KPNYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPPTP
                     EVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPGEPTPPTPEVPAEPGKPVPPAKEE
                     PKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKAQSKKSELPETGGEESTNKGMLFGGL
                     FSILGLALLRRNKKNHKA"
BASE COUNT          149 a          132 c          141 g          272 t
ORIGIN      
        1 attctaactt tatctctcag ttcgttatca atcatcagac ataaacagat gaagcaatca
       61 gaaaacactc taattttcta ttagaaattt gatttaatat aaaaaaacag gcttacttca
      121 tataatttat gaaataaacc cgtcaatttt agattgatta tgctttgtga ttctttttat
      181 ttctgcgtaa taatgctaaa cctagaatgc tgaataatcc gccgaacaac atacctttgt
      241 ttgttgattc ttctccacct gtttcaggta gttcagattt cttagattgt gcttttttag
      301 ttggtgccac tgctttaacc ttttcattga tttcaatgac aggtgttact accttacctt
      361 gttccactgg cttagaaggt tttttaggtt cttctttggc tggtggtact ggtttaccag
      421 gttcagctgg tacttctggt gtcggtggcg ttggttcgcc tggctcactc ggcacttctg
      481 gtgtcggtgg tgttggtgtt tccggctcac tcggtacttc tggtgtcggt ggtgttggtg
      541 tttccggctc acttggtact tctggtgtcg gtggtgttgg cggcgttgta tcttcttcaa
      601 tcgtttgttg accttcattt tgaccactta cttttggaag tgtatcttct tcaaaatcaa
      661 cactgttgtg tccaccaaat tgataattag gttt
//
LOCUS       DAPIDM010000276          689 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000276 DAPIDM010000000
VERSION     DAPIDM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.284"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>689
                     /locus_tag="RJE42_002773"
     CDS             <1..>689
                     /locus_tag="RJE42_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDV5878110.1"
                     /translation="IGKGIGFNKKEGMVLNDQTNTIEKIYKLESEQQKAHYKSLVEIA
                     DDNVLQVIIDSLNFISNTAMNVDSKQLVVSLTDHIIFAYKRLKQNQVISNPFVMETMQ
                     LYSDAYHIAKQVIDQLNAALDVHFPEDEIGFIALHIASNTEDLSMHEMTLINNVIKKG
                     IDIIESDLVTTVDKESLQYQRFIRHVQFLIRRLRRKEYIHAQDDFVSMIKNHYPICYN
                     TAYKILTMIQK"
BASE COUNT          271 a           84 c          110 g          224 t
ORIGIN      
        1 taatcggtaa aggtattggt tttaacaaaa aagagggaat ggtgttaaac gaccaaacta
       61 atacaataga gaaaatttat aaattagaga gtgagcaaca aaaagcacat tataaaagtt
      121 tagttgaaat cgctgatgat aatgtattac aagtaattat tgattcgtta aattttattt
      181 ctaatactgc gatgaatgtt gattcaaagc aacttgtagt ttcattaacg gatcatatta
      241 tatttgcata taaacgctta aaacaaaatc aagttattag caatccattt gttatggaaa
      301 ctatgcagtt atatagtgat gcatatcata ttgctaaaca ggtgattgat cagttaaatg
      361 cagcattaga tgtacatttt cctgaagatg agataggatt tattgcatta catattgcat
      421 ctaacactga agatttatct atgcatgaga tgaccttgat caataatgtt attaaaaaag
      481 gtatagatat cattgaatca gaccttgtga caactgttga taaggaatca ttacaatacc
      541 aacgttttat aagacatgta caatttttaa ttcgccgatt aagaagaaaa gaatatatac
      601 atgcacaaga tgattttgtg tcgatgataa aaaatcacta tccgatttgc tataatacag
      661 catataaaat tttaactatg atacaaaaa
//
LOCUS       DAPIDM010000277          678 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000277 DAPIDM010000000
VERSION     DAPIDM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.285"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT          142 a          160 c          106 g          270 t
ORIGIN      
        1 agaaaagcag tcataaattg atttctgatt gaaatcatct tatgactgct ttttattatg
       61 ctttacatta ttgaggttgt acgctttgta taccatcgta tatttctttg agcctaaaat
      121 cttctttgcc tttacttttt tactttgcta ctttagacat aaaaaaagag accttgcggt
      181 ctcaacttgc ctggcaacgt tctactctag cggaacgtaa gttggctacc ttcgtcgcta
      241 agaacctttc ttgacttgtg acaatcgctt gcttctttcc tctccttcgg ctctcgctta
      301 ctcatttagc tctactaaac tcgttgcgct cttttctcgt ttcgtcagat tcaaacattt
      361 tcacttcgtc aagccatttt tctttgtgtt tgctttttta ctttgctact ttagacgtaa
      421 aaaaaagaga ccttgcggtc tcaacttgcc tggcaacgtt ctactctagc ggaacgtaag
      481 ttggctacct tcgtcgctaa gaacctttct tgacttgtga caatcgcttg cttctttcct
      541 cttcttcggc tctcgcttac tcatttagct ctactaaact cgttgcgctc ttttctcgtt
      601 tcgtcagatt caaacgtttt cacttcgcca agccattttt ctttgtgttt gctttttatt
      661 ttgacgtttt agacataa
//
LOCUS       DAPIDM010000278          624 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000278 DAPIDM010000000
VERSION     DAPIDM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.286"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..532
                     /locus_tag="RJE42_002774"
     CDS             <1..532
                     /locus_tag="RJE42_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDV5878111.1"
                     /translation="QDFDEIVDYCRDRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLP
                     MKRVDDIKAYIQGDVPKVMGVDYVANITEARIDLNGVFNDNVDATTSKPFFLEFMAKD
                     VSKGNAIKALCHKLGYSVDQVIAFGDSMNDKSMFEVAGLAIAMGNASDELKQYADEIT
                     LDHNENGIPHALKKLL"
BASE COUNT          210 a           76 c          134 g          204 t
ORIGIN      
        1 gcaggatttc gatgaaattg tagattattg tcgagataga ggctttttcg ttcttacata
       61 tcatgatggt caaattattt acgacagcga acatgaatat atgaatattg aagcggaatt
      121 aactggttta ccgatgaaac gtgttgatga tatcaaagca tatattcaag gcgatgtacc
      181 caaggtcatg ggagttgatt atgtagcgaa tattactgaa gctagaattg atttaaatgg
      241 cgtgttcaat gataatgtag atgctacgac aagtaagcca ttcttcttag aatttatggc
      301 taaagatgtt tcaaaaggta atgcaattaa agcgttatgt cacaaattgg gatattcggt
      361 tgatcaagtc attgcttttg gtgatagtat gaatgataaa tcaatgtttg aagtcgcagg
      421 tctagctatt gctatgggga atgcatcaga tgaacttaag caatatgcag atgaaattac
      481 gttggaccat aatgaaaatg gtattccaca tgcgctcaaa aaattgttat aaattttaaa
      541 ataagcctaa gcacaggata tttgaataag atatcttgtg ttaaggcttt ttatttatgt
      601 gaaaatgcgt tagctataat atgg
//
LOCUS       DAPIDM010000279          620 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000279 DAPIDM010000000
VERSION     DAPIDM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.287"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..>620)
                     /locus_tag="RJE42_002775"
     CDS             complement(<1..>620)
                     /locus_tag="RJE42_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV5878112.1"
                     /translation="LSIYLPESYNQLVKYNVILCFDGLDFLRFGRIQRTYESLIKEAR
                     IDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQSVGKEILPFIDSTFSTLKVGNARLL
                     VGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEKVLDKLNQCANKEQLTIWHVIGLNE
                     KDFTLPTNGKRADFLTPNRELAEQIKKYNITYYYDEFEGGHQWKDW"
BASE COUNT          201 a          122 c           86 g          211 t
ORIGIN      
        1 ttccaatctt tccattggtg accaccttca aattcatcgt aataataagt tatattatat
       61 ttcttaattt gttcagctaa ttctctattc ggtgttaaga aatcagcacg cttaccattt
      121 gttggtaaag tgaaatcttt ttcattcaga ccaatgacat gccaaattgt caattgttct
      181 ttattagcac attgatttag cttatctaat actttttcat ctgaatgtgg acttaacatt
      241 gctacacgac taaaaatcgt tggataggtc aacgccgtta ataaggcaat actacccgct
      301 aaactatccc ctactaataa ccttgcatta cctactttca gtgtagaaaa cgtcgaatca
      361 ataaatggca atatttcttt accgactgat tgaatagtta aatgagaacg acttccttgt
      421 ggatgaaatt cctctctacg cttatcaacg tcttcataat ggaatccgac aataatcgca
      481 tcatcaatac gcgcttcttt gattaacgat tcatatgtac gttgtattct cccgaaacgt
      541 aaaaaatcta atccgtcaaa gcaaagaatg acattatatt taacaagttg attataagat
      601 tctggcaaat aaatacttag
//
LOCUS       DAPIDM010000280          613 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000280 DAPIDM010000000
VERSION     DAPIDM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.288"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>613
                     /gene="spa"
                     /locus_tag="RJE42_002776"
     CDS             <1..>613
                     /gene="spa"
                     /locus_tag="RJE42_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDV5878113.1"
                     /translation="SGGVTPAANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKD
                     DPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEEQRNGFIQS
                     LKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQS
                     LKDDPSQSANLLAEAKKLNESQAPKADNKFNKEQQNAFYEILH"
BASE COUNT          244 a          128 c           95 g          146 t
ORIGIN      
        1 tatctggtgg cgtaacacct gctgcaaatg ctgcgcaaca cgatgaagct caacaaaatg
       61 ctttttatca agtgttaaat atgcctaact taaacgctga tcaacgtaat ggttttatcc
      121 aaagccttaa agatgatcca agccaaagtg ctaacgtttt aggtgaagct caaaaactta
      181 atgactctca agctccaaaa gctgatgcgc aacaaaataa cttcaacaaa gatcaacaaa
      241 gcgccttcta tgaaattttg aacatgccta acttaaacga agagcaacgc aatggtttca
      301 ttcaaagtct taaagacgat ccaagccaaa gcactaacgt tttaggtgaa gctaaaaaat
      361 taaacgaatc tcaagcaccg aaagctgaca acaatttcaa caaagaacaa caaaatgctt
      421 tctatgaaat cttgaacatg cctaacttga acgaagaaca acgcaatggt ttcatccaaa
      481 gcttaaaaga tgacccaagt caaagtgcta accttttagc agaagctaaa aagttaaatg
      541 aatctcaagc accgaaagct gataacaaat tcaacaaaga acaacaaaat gctttctatg
      601 aaatcttaca ttt
//
LOCUS       DAPIDM010000281          580 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000281 DAPIDM010000000
VERSION     DAPIDM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.289"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            1..495
                     /locus_tag="RJE42_002777"
                     /pseudo
     CDS             1..495
                     /locus_tag="RJE42_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          231 a           68 c           98 g          183 t
ORIGIN      
        1 atgttaaaaa ttatcttata tgcatacact caatctgtat tttctggtcg tagaatagag
       61 aaattacttc atgacagtat tcgaatgatg tggttagctc aaaatcaaac accttcttat
      121 aaaactatta atcgttttag agtaaatcct aatactgatg cattaattga atctttattt
      181 atccagtttc atagtcaatg tttaaagcaa aatcttattg atgataattc aatttttatc
      241 gatggtacaa aagtagaagc tagtgccaat aggtatacat ttgtgtggaa gaaaagtatt
      301 caaaatcacg aattgaaatt gaatgaaaat tcaaaagcat tatatcgtga cttagttgaa
      361 gaaaaaataa tactagagat taaagaagat ggagatagcg atttaacaac agaagaaatt
      421 gatttagttg gtagtcattt agataaagaa atcgaagatt taaatcattc tattgagaac
      481 gaaaactgta ctcaaagagg aataaataaa gttaaacgag agctaggttt tgtattaatg
      541 gcacttaata taaggaaaat agcagctcaa cgagctgtac
//
LOCUS       DAPIDM010000282          568 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000282 DAPIDM010000000
VERSION     DAPIDM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.290"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            49..384
                     /locus_tag="RJE42_002778"
     CDS             49..384
                     /locus_tag="RJE42_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV5878114.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGEALAEALHPIEAWAQSYV
                     DLTDQRTAK"
BASE COUNT          201 a           74 c          107 g          186 t
ORIGIN      
        1 aaagggtgtt cgtttacatt ctaaatttat tgatgagagg tgaagatgat ggaagtatgt
       61 ccgtatctcg aagaaacttt taaaatactt ggtagaagtt ggaatggatt aatcattaat
      121 tatctctcaa gatgtaatga ctgttcagca cacttttccg atatgaaaag agatttgaaa
      181 acaataacgc cacgtgcttt aagtcttaag ttgtcagagc ttgcacaatg ggagttagtt
      241 gagaagcaaa tcatttctac gagtccagta caaattattt atgtactaac tgaaaaaggt
      301 gaagcgttag cagaggcttt acatccaatt gaagcatggg cgcaatcata tgtcgattta
      361 acagatcaac gtactgctaa ataattacat gacgtttaaa catagcattg attataacta
      421 tttctaagtc ttcgcattat tcgcgatgat gtgggaatag ttatttttat ttaaaaatat
      481 aaaaaattag atgcagcaaa attttaaagc attttatttt gaacatatta aaaaggagcg
      541 tatcataatg gaatgtaatg tttatatc
//
LOCUS       DAPIDM010000283          559 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000283 DAPIDM010000000
VERSION     DAPIDM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.291"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(188..>559)
                     /locus_tag="RJE42_002779"
     CDS             complement(188..>559)
                     /locus_tag="RJE42_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDV5878115.1"
                     /translation="EEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQS
                     VENVVENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNH
                     ILSICNEHELHVSGKYISSHF"
BASE COUNT          176 a           96 c           72 g          215 t
ORIGIN      
        1 taatttattt ggatgaaata taattcttta aactctattg attaatcaaa tatgtatcta
       61 tcaaaattcg gcttatttat tcactctgac atatccaaat acgcaaaaag actatttcta
      121 ccttgtatcg atagaaatag tctttttata cttaattggt ttaatttttc gtaaaaaatt
      181 atactcttta aaaatgggat gaaatatatt ttcctgaaac atgtaattca tgttcgttac
      241 aaattgataa aatatggtta agtgtcgttg caaatgacat tttctcaaaa agcgcttcac
      301 tatcaattgg tacttgtgtg tgaatttcag ccagtcgctt cgataaatat agttcatcta
      361 aattgtcatt aatcttatta cgttgcccag ctgataatgc atcaatgttt tcaaccacat
      421 tttcaacgct ttgatattgc tgaattaact taatagccgt tttctcacca attcctttaa
      481 cacctgcata tccatctgct gtatcaccca taaacgcttt aatatcgatt agttgttgtg
      541 gttcaagggc atattcttc
//
LOCUS       DAPIDM010000284          525 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000284 DAPIDM010000000
VERSION     DAPIDM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.292"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..233
                     /locus_tag="RJE42_002780"
     CDS             <1..233
                     /locus_tag="RJE42_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV5878116.1"
                     /translation="DMNLEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHE
                     SYDETMSTLIAEKLRDPNYLISCSDNFVVKNR"
     gene            complement(415..503)
                     /locus_tag="RJE42_002781"
     tRNA            complement(415..503)
                     /locus_tag="RJE42_002781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Ser,seq:gct)
BASE COUNT          196 a           77 c           93 g          159 t
ORIGIN      
        1 ttgatatgaa tctagaacct gggcaacgag taatgttaga tcaaattgat ttgactaaga
       61 gttatagagt tacaatgact gactattgtt atcgtaacta caagaattat ttaaaaaatg
      121 ctattataca tgaatcatac gatgaaacaa tgagtacatt aattgcagag aagttaagag
      181 atccgaatta tcttatttca tgtagtgata attttgtagt taaaaacagg tgaatattgc
      241 tatgtaataa attaattata agtttaacaa gtataaaaac ctttcttaga gaagattatc
      301 taggaaaggt ttttagaatt ataaaattga tataaaagaa cagtgaaatt ctcattcatt
      361 atatatataa gtaaacaaaa aagcttaaat ggtatatacc atttaagcaa gtaacggaaa
      421 cggagggatt cgaaccctcg cgccgctttc gcgacctaca cccttagcag gggcgcctct
      481 tcagccaact tgagtacgtt tccaatggct ccacaggtag gactc
//
LOCUS       DAPIDM010000285          521 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000285 DAPIDM010000000
VERSION     DAPIDM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.293"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT          138 a          112 c           68 g          203 t
ORIGIN      
        1 gtctattgta taataattaa tttccttttt gtatttcgat ttaaaagata ttagacataa
       61 aacctaaaaa cagcagtaag atgatttatg attaaaaact atcttactgc tgttcacttt
      121 ttataatact tctgaatgtc ttcgcttata cttctagtca cagatttaaa caatcataag
      181 tgcacattat taaattatca atttcacact tcgccaagcc atctttcttt gtgttaacct
      241 ttatttcaac gttttagaca taaaaaaaag agaccttacg gtctcaatgc ggctcatcgc
      301 atccattttt ttgcctggca acgttctact ctagcggaac gtaagttggc taccatcgtc
      361 gctaagaacc tttcttgact tgtgacaatc gcttgcttct ttcctctcct tcggctctcg
      421 cttactcatt tagctctact aaactcgttg cgctcttttc tcgttttgtc agattcaaac
      481 gttttcactt cgtcaagcca tttttctttg tgttaatttt t
//
LOCUS       DAPIDM010000286          477 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000286 DAPIDM010000000
VERSION     DAPIDM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.294"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..>477)
                     /locus_tag="RJE42_002782"
     CDS             complement(<1..>477)
                     /locus_tag="RJE42_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV5878117.1"
                     /translation="FDFMFQPSINQQEILTLKSMHFLEDSINICFLGNSGVGKTHLAI
                     SLGIEACKQNIKTRFYTFKELIDLLTVSDSKGIINKTLKQLSRIELLIIDEIGYTPIT
                     KEQADLFYQLMSLRYEWKSTIITTNIPFSSWGESFSNKIVSAAIIDRLVHHSKVFK"
BASE COUNT          156 a           70 c           67 g          184 t
ORIGIN      
        1 ttttaaatac ttttgaatgg tgaactaacc gatcaattat agctgctgat acaatcttat
       61 tactaaatga ctctccccaa cttgaaaatg gaatattagt cgtaattatt gttgatttcc
      121 attcatacct tagtgacatt aactggtaga ataaatcagc ttgttctttg gtgatgggag
      181 tataaccaat ttcatcaatg ataagtaact ctattctgct taattgtttt aatgttttat
      241 taatgattcc tttggaatct gagacagtta ataagtctat taattctttg aaagtataga
      301 atctagtctt tatattttgc ttacatgcct ctattcctaa tgagattgct aggtgcgttt
      361 taccaacacc actattacct aagaaacaaa tatttatact atcttctaaa aaatgcatag
      421 attttaaagt gagtatttct tgttgattta tactaggttg aaacatgaaa tcaaaat
//
LOCUS       DAPIDM010000287          467 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000287 DAPIDM010000000
VERSION     DAPIDM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.295"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(84..>467)
                     /locus_tag="RJE42_002783"
     CDS             complement(84..>467)
                     /locus_tag="RJE42_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDV5878118.1"
                     /translation="QDHQGYWFLRFRAALVPVANLGSSLSYMIIMLGFILTAIGSAFG
                     STALWIGAGLMSLAVLFSIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAA
                     MTYVAATAVAVAELVRIILIARSSD"
BASE COUNT          163 a           97 c           70 g          137 t
ORIGIN      
        1 agcacaaaaa aactagcaat cacacaaaat gtgattagct agttaataag tgtctaattt
       61 aagttaattg ttaatctata agattaatca cttgaacgcg caatcaaaat aatacgtaca
      121 agctctgcta cagcgacagc agttgctgca acataagtca ttgctgctgc agataatact
      181 ttacgcgcat gtttgtattc cttttcattt acaatgttca atgccgtaat ttgtttcatc
      241 gctcttgaac tcgcatcaaa ctcaactggt aacgtaacaa ttgagaataa taccgctaat
      301 gacattaaac cagcaccaat ccataaagca gttgaaccaa atgcactacc tatcgctgtt
      361 aagataaaac ctaacatgat gatcatataa cttaatgaac tccctaggtt tgcaacaggt
      421 actaatgctg ctctgaatct taagaaccaa tatccttggt gatcttg
//
LOCUS       DAPIDM010000288          460 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000288 DAPIDM010000000
VERSION     DAPIDM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.296"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            14..>460
                     /locus_tag="RJE42_002784"
     CDS             14..>460
                     /locus_tag="RJE42_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDV5878119.1"
                     /translation="MSTNQTFLIFVIAIIILTSVIGIVGRYMSRQRLLKSMVTLWQTI
                     SPLESFIRPNSHFDYEYKLYKDKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPD"
BASE COUNT          163 a           67 c           69 g          161 t
ORIGIN      
        1 taaggagata gttatgagca caaatcaaac atttttaata tttgttatag caattattat
       61 acttacatcg gtaataggaa ttgttggacg atacatgagt cgtcaacgtc tattaaaatc
      121 tatggtaaca ttatggcaaa cgatttctcc attagagtct tttatcagac cgaactcaca
      181 tttcgactat gagtataagc tctacaagga taaatttgaa tcacattctt tagttgatga
      241 taaaacttgg tccgatttaa atatgaatgc tatctttcat aagatgaatt ataatttaac
      301 agctattggc gaaatgaaac tatatgcctg tttacgtgga atgctttcaa ttacaaacaa
      361 atcattactt agtttattta atgataatgc tgaatttaga aaaaacgtaa catatcattt
      421 agctttgatt ggtaaaactg tttatccaac atttccagac
//
LOCUS       DAPIDM010000289          458 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000289 DAPIDM010000000
VERSION     DAPIDM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.297"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            12..87
                     /locus_tag="RJE42_002785"
     tRNA            12..87
                     /locus_tag="RJE42_002785"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Asp,seq:gtc)
     gene            104..176
                     /locus_tag="RJE42_002786"
     tRNA            104..176
                     /locus_tag="RJE42_002786"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Phe,seq:gaa)
     gene            190..265
                     /locus_tag="RJE42_002787"
     tRNA            190..265
                     /locus_tag="RJE42_002787"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:223..225,aa:His,seq:gtg)
     gene            281..354
                     /locus_tag="RJE42_002788"
     tRNA            281..354
                     /locus_tag="RJE42_002788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:Gly,seq:tcc)
     gene            369..443
                     /locus_tag="RJE42_002789"
     tRNA            369..443
                     /locus_tag="RJE42_002789"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Asn,seq:gtt)
BASE COUNT           95 a          111 c          114 g          138 t
ORIGIN      
        1 ttaatttaat aggtctcgta gtgtagcggt taacacgcct gcctgtcacg caggagatcg
       61 cgggttcgat tcccgtcgag accgccatta ttattaccat tacggttcag tagctcagtt
      121 ggtagagcaa tggattgaag ctccatgtgt cggcagttcg actctgtcct gaaccattct
      181 taattcatgg cggttgtggt gaagtggtta acacatcgga ttgtggttcc gacattcgag
      241 ggttcgatcc ccttcagccg ccccataatc gtttacatta gcgggtgtag tttaatggca
      301 aaacctcagc cttccaagct gatgttgtgg gttcgattcc catcacccgc tccattattt
      361 tctattattc cacagtagct cagtggtaga gctatcggct gttaaccgat cggtcgtagg
      421 ttcgagtcct acctgtggag ccatggctcc ttggtcaa
//
LOCUS       DAPIDM010000290          443 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000290 DAPIDM010000000
VERSION     DAPIDM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.298"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            155..396
                     /locus_tag="RJE42_002790"
                     /pseudo
     CDS             155..396
                     /locus_tag="RJE42_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          173 a           70 c           57 g          143 t
ORIGIN      
        1 aaatacttaa aaaataagac actttgccca acttgcacat aaatgtaaaa ttcaataaaa
       61 tgaattttct gtgttgggtc ccgtcgtata atttaataaa taccactaaa ctaaattaac
      121 gaggtgcctt atgtataaaa attataacgt gacccaactt aagccaggat acaatttaca
      181 aatagcgaca aattctcaat ttgttttatc ctatgaccta tttcaaaacc caacagatac
      241 tagaacatta attccatttt taacaatgat tcaaaatacc ttcgttattt accagagtat
      301 attgtagttg atggaggtta tggtagtgag caaaactata tggctattat agatgatttt
      361 aataaaacgc cacttattac atatggcatg tttatttaag ataaaactag aaagtttaaa
      421 agtgacattt tcaacactca aaa
//
LOCUS       DAPIDM010000291          414 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000291 DAPIDM010000000
VERSION     DAPIDM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.300"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..349)
                     /locus_tag="RJE42_002791"
     CDS             complement(<1..349)
                     /locus_tag="RJE42_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDV5878120.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLI"
BASE COUNT          148 a           61 c           66 g          139 t
ORIGIN      
        1 ctatcaataa cataataggt ggcataaaat aaccaccaat cgttgtaagc cattgaccag
       61 ctagatgtct agattgtgta attgcgaatc cttgttgtaa tgtctgttgt cgctctcgtg
      121 gacttgttac aatgactaaa tcttttgcac ggccaccagc gagtttatta aacagtacat
      181 gaccaaattc atgtgttaaa acagggatat agtttaaaat gacatctaaa tagttcaaaa
      241 caggcttatg tctatattga tgaatagcaa tataacaagc tgcaacaata acgataatgt
      301 atatattaag ttgaattgtc gtattaaaaa agtttgataa ataattcatt gttaacctca
      361 tataagatat taatttaaag tttgcttatc acttattata aatgatattg gcat
//
LOCUS       DAPIDM010000292          404 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000292 DAPIDM010000000
VERSION     DAPIDM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.301"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>404
                     /locus_tag="RJE42_002792"
     CDS             <1..>404
                     /locus_tag="RJE42_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDV5878121.1"
                     /translation="QRLGEPETEIVKGRVRYEQNNKEYDVFHKNHIYTTVFYDKHRRN
                     NVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQNFDLVNAERKQHQLSTLKYSKQNS
                     ETARKHSKDMAKNHYFDHTNLKGQSPFDRLKK"
BASE COUNT          167 a           52 c           69 g          116 t
ORIGIN      
        1 ggcaaagatt aggtgaacca gaaacagaga ttgttaaagg tagagtgcgt tacgaacaaa
       61 ataataaaga atatgatgtt ttccataaaa atcacattta tacgacggta ttttatgata
      121 agcatcgacg taataatgta acagctgttt tacaagtaag tgatgctatg gaaaatagat
      181 taaaagaaca atatggagca ccttcgaaat cgcttgcaga tagttttgaa ctacaaaatt
      241 ttgatttagt taatgctgaa agaaaacaac atcaattatc tacattgaag tattctaaac
      301 agaattctga aactgctcgt aagcatagta aagatatggc aaaaaatcat tattttgatc
      361 atacaaattt aaaaggtcaa tcaccatttg atcgattgaa aaaa
//
LOCUS       DAPIDM010000293          378 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000293 DAPIDM010000000
VERSION     DAPIDM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.302"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..318
                     /locus_tag="RJE42_002793"
     CDS             <1..318
                     /locus_tag="RJE42_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HDV5878122.1"
                     /translation="PKFNTTPKYIKFRHDYNIVEYNDGTFEYGARPQFNKPAAKTEAT
                     IKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYNVKKMILQERIDQVLKQG
                     LVR"
BASE COUNT          163 a           51 c           56 g          108 t
ORIGIN      
        1 ccaaaattta atacaacacc taaatatatt aagtttagac atgattataa catcgtggaa
       61 tataatgacg gtacattcga atatggtgca cgtccacaat ttaataaacc agcagcgaaa
      121 actgaggcaa ctattaaaaa agaacaaaaa ttgattcaag cccaaaatct tgtgagagaa
      181 tttgaaaaaa cacatactgt gagcgcacat agaaaagcac aaaaggcagt caacttagta
      241 tcatttgaat acaatgttaa gaaaatgata ttgcaagagc gaattgatca agtattaaag
      301 caaggattag tgagataata cttctgtcat tattataagt tcaaaataat ttaatattat
      361 attatttttt attaataa
//
LOCUS       DAPIDM010000294          378 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000294 DAPIDM010000000
VERSION     DAPIDM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.303"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..308)
                     /locus_tag="RJE42_002794"
     CDS             complement(<1..308)
                     /locus_tag="RJE42_002794"
                     /inference="COORDINATES: protein motif:HMM:NF024260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV5878123.1"
                     /translation="MNAAKSYSLSRSSEYVMDPLSEVLSLLNTRHSVFAGLKAGGDWA
                     IDFPPPDGIKFNAIVQGGCWLTVEDVAQPIWLESGDCFLLSRKRAFSLRSELSLPAIP
                     S"
BASE COUNT           89 a          106 c          116 g           67 t
ORIGIN      
        1 gaaggaatgg ctggcagcga aagctcgctg cgaagtgaaa acgcacgttt gcgcgacagc
       61 aggaagcaat ccccgctttc cagccagatg ggctgcgcca cgtcttccac ggtcagccag
      121 cagcctccct ggacgatggc attgaacttg atgccgtcgg gtggggggaa gtcgatggcc
      181 cagtcaccgc ccgctttcaa tcccgcgaag acagagtgcc gggtattcag gagggaaagc
      241 acttccgata gcggatccat gacgtattca gacgatctcg acaatgaata ggactttgct
      301 gcattcacag tccgaagtcc agcgtcgaca atgcgggcca ggggttctcc agaatgcagg
      361 ccaggctcaa tcggggca
//
LOCUS       DAPIDM010000295          365 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000295 DAPIDM010000000
VERSION     DAPIDM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.304"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            15..212
                     /locus_tag="RJE42_002795"
     CDS             15..212
                     /locus_tag="RJE42_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878124.1"
                     /translation="MNCYDEIYNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT          153 a           35 c           61 g          116 t
ORIGIN      
        1 gcctcatcta atttatgaat tgctatgatg aaatatacaa tacaatcaaa aaattgatag
       61 aaaacaaaga gatatcgagt tatcaaatta ataaagatac tgggataagt tacggtaata
      121 ttaatgctat gcgccgtgga gaaagaagaa tagaaaattt aagcttaaag aatgcaaaga
      181 tcttatatga atatgcgaaa aaggtattat aatataagca ttgagttcaa taggcataca
      241 ataactgccc aaactagtga ctgagtgggt gtttttatag tattaaaagt tttctaaaag
      301 tgaaagaaat catgtttagt gatttactta tgtttttcaa aatagtataa taattttata
      361 taata
//
LOCUS       DAPIDM010000296          353 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000296 DAPIDM010000000
VERSION     DAPIDM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.305"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..246
                     /locus_tag="RJE42_002796"
     CDS             <1..246
                     /locus_tag="RJE42_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV5878125.1"
                     /translation="YFNIIGGMDYEKCINKFDGIDKILCKSLISGINDGSHFISDDFV
                     ITFDEASMRNYKRVFKLVFEKLGHIQHFEMMSKNKIK"
BASE COUNT          145 a           27 c           58 g          123 t
ORIGIN      
        1 tattttaata ttataggggg catggattat gaaaaatgta ttaataaatt tgacggtata
       61 gacaaaatat tatgtaaatc acttatttca ggaataaatg acggatcaca ttttataagc
      121 gatgattttg ttattacctt tgatgaagca agtatgagaa attataaaag agtctttaag
      181 ttagtctttg aaaaacttgg acatatccaa cactttgaaa tgatgagtaa gaataagatt
      241 aaataattta agtaggtagt tggttaatga aatatgaaaa attcgactaa taaatgaatg
      301 attaaagtat attcattgga ctttaatatg aaaatataca attaatgtac tta
//
LOCUS       DAPIDM010000297          351 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000297 DAPIDM010000000
VERSION     DAPIDM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.306"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT          132 a           56 c           31 g          132 t
ORIGIN      
        1 tagtaacaat atattctccc attatcatca cctatttttc tattattaag attatatatc
       61 ggaaatgtct aacttaaaag tataataatt taaatacttg tgttctatta cacgcctatc
      121 ctatatgata tatcttaatt taatttcgat gcctctcaaa gtggaataac tttaataaaa
      181 aaatctgact cccatatttg caaataatgc tatttattta tatattatct gaattaatac
      241 tcaatacaaa ctaaaacgta ctattaaatt gtgcaaagct aaaataaatt tatattcatc
      301 tatccaacaa tatgtcttat caatggtata gtctttgcac accaatgaag g
//
LOCUS       DAPIDM010000298          330 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000298 DAPIDM010000000
VERSION     DAPIDM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.307"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..>330)
                     /locus_tag="RJE42_002797"
     CDS             complement(<1..>330)
                     /locus_tag="RJE42_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDV5878126.1"
                     /translation="VFNNLFYQFSKDANFEPIACRPYRPQTKGSVESLAKFVEQRLRP
                     YDYEFYDAVELIELVDDLCHELNHLEISQVTEQRPIDVFNYEEKEHLNFFNAKLLDTY
                     IENECIR"
BASE COUNT          111 a           52 c           51 g          116 t
ORIGIN      
        1 ttcttataca ctcattttcg atgtaagtat ctagtaactt ggcattaaaa aaatttaaat
       61 gttctttttc ttcataattg aaaacgtcga taggtcgttg ttctgttact tgtgaaattt
      121 ctaagtgatt caattcgtga cataaatcgt ctactagctc aataagttct acagcatcat
      181 aaaattcata atcatagggt cttaagcgct gttcaacaaa tttagctaat gattcaacag
      241 acccttttgt ttgagggcga tagggtctac aagcaatagg ttcaaagttg gcatccttac
      301 taaattgata aaataaatta ttaaaaacga
//
LOCUS       DAPIDM010000299          324 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000299 DAPIDM010000000
VERSION     DAPIDM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.308"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..248
                     /locus_tag="RJE42_002798"
     CDS             <1..248
                     /locus_tag="RJE42_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDV5878127.1"
                     /translation="KYSVPTKYIGEEVQVIFNNSTDELLIYYDGELIRRHNLSERKFN
                     YIVEDMSEILKSDVFKHKDDKEILTYIENSLLLYDEI"
BASE COUNT          138 a           37 c           50 g           99 t
ORIGIN      
        1 gtaaatactc ggtacctact aaatacattg gtgaagaagt acaagtgata ttcaacaatt
       61 ctactgatga attgctgatt tattatgatg gtgaattaat aagacggcat aatctatccg
      121 aaagaaaatt taattatatc gttgaagata tgagtgaaat attaaaatca gatgtattta
      181 aacataagga tgacaaagaa attcttacgt atatagaaaa ttcattatta ctgtatgacg
      241 aaatttagga ggactttcga tgaatacgaa ccatcaaaaa ttactcaata actttgaaat
      301 attaaaacta aaaaaattca agga
//
LOCUS       DAPIDM010000300          316 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000300 DAPIDM010000000
VERSION     DAPIDM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.310"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..201)
                     /locus_tag="RJE42_002799"
     CDS             complement(<1..201)
                     /locus_tag="RJE42_002799"
                     /inference="COORDINATES: protein motif:HMM:NF016262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HDV5878128.1"
                     /translation="MHIPPEDSLIEYPSDFPIKVMGKQHPDFAQTLTEVVLRFDPGFD
                     AATVEMRPSKGGNYMGLTFTVRA"
BASE COUNT           55 a           90 c          117 g           54 t
ORIGIN      
        1 ggcgcgcacc gtgaaggtca ggcccatgta gttgccgccc ttgctgggcc gcatttcgac
       61 ggtggcggca tcaaagcccg ggtcgaagcg cagcacgact tcggtcagcg tctgcgcaaa
      121 atcgggatgc tgcttgccca tgaccttgat gggaaagtcg ctggggtact cgatgaggga
      181 gtcttcgggc ggaatatgca tgatggatgg cctggagcgt caattcatct atatgggggc
      241 aggcatcgcc caggcaaggc ccgcccgtca gggcgggcga cgcggggggc tggtcacagc
      301 gcccccgcag ggccgg
//
LOCUS       DAPIDM010000301          312 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000301 DAPIDM010000000
VERSION     DAPIDM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.311"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT           66 a           82 c           82 g           82 t
ORIGIN      
        1 gttgatggaa gtctctcatt tcctcgtacg agacgtctcg tccagtggaa acgtgaccag
       61 cttctcgcgc ctgtttaact gtctcgtagt accgctctgg ctcggccaag attaggtcga
      121 agatgcgctc actgacgcta gctaaaaatt ggcgtacatt ttcccgttgg gcagggtgcc
      181 gtacggcgaa caacgcagcc aaattcatta cgtaattcca gttctcgtcc gagggtatgg
      241 aacggctgcg ttcgatctct cggagagctt tgcacgctag gccttcgaat tgtcccaaag
      301 cattgtctaa gg
//
LOCUS       DAPIDM010000302          311 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000302 DAPIDM010000000
VERSION     DAPIDM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.312"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>311
                     /locus_tag="RJE42_002800"
     CDS             <1..>311
                     /locus_tag="RJE42_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV5878129.1"
                     /translation="YEGKYTILREYCKNKKQNETKKATIRVETNPGIAAQVDWKEDMV
                     MHDKFGRTYQFNIFLYVLHYSKMKYITLTWDRKQDTLFECLKDAFEYTEGVPKEIWFD
                     N"
BASE COUNT          126 a           46 c           53 g           86 t
ORIGIN      
        1 gttatgaagg taaatatact attctacgag aatactgtaa aaataaaaaa caaaatgaga
       61 ctaaaaaagc aactatacgc gtagaaacta atcccggtat agctgctcaa gtagactgga
      121 aagaagatat ggtaatgcat gataagtttg gcagaactta tcaattcaac atctttcttt
      181 acgttctaca ctattcaaaa atgaagtata tcacattaac ttgggataga aaacaagata
      241 ccttatttga atgtttaaaa gacgcttttg aatacaccga aggggttcca aaagaaatct
      301 ggttcgataa t
//
LOCUS       DAPIDM010000303          310 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000303 DAPIDM010000000
VERSION     DAPIDM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.313"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(14..118)
                     /locus_tag="RJE42_002801"
     CDS             complement(14..118)
                     /locus_tag="RJE42_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878130.1"
                     /translation="MNGLNSVIGAMIIIDKNATTVTMNFLIYITYYKY"
BASE COUNT          130 a           41 c           45 g           94 t
ORIGIN      
        1 acggtgatag agattaatat ttatagtagg tgatgtaaat taaaaaattc atagtaactg
       61 ttgttgcgtt tttatcaatt attatcattg cgccaataac agaatttaaa ccattcattc
      121 atttacaaaa tgaagtaaga caatatattg acattcacat caataaagaa acaatttctg
      181 cagaaaataa attggatacg ccgaagaaac aacaatttgc ctttaataac attcagatga
      241 atatgtcaaa gtcagatgtt gagaaaacat tgaataaacc caaaagagtt acatttaatg
      301 aatatggcac
//
LOCUS       DAPIDM010000304          295 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000304 DAPIDM010000000
VERSION     DAPIDM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.314"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>295
                     /locus_tag="RJE42_002802"
     CDS             <1..>295
                     /locus_tag="RJE42_002802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV5878131.1"
                     /translation="QQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPEKPNKPIY
                     PEKPKDKTPPTKPDHSNKVKPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTG"
BASE COUNT          139 a           61 c           40 g           55 t
ORIGIN      
        1 caacaagtaa aatacacagt cgaggaatta acaaaggtca aaggttatac aacacatgtg
       61 gataacaatg atatgggcaa cttgattgtg acgaataaat atacgccaga aaaaccgaat
      121 aaaccaatct atcctgaaaa accaaaagac aaaacaccac caactaaacc tgatcattct
      181 aataaagtta aaccaactcc cccagataag ccatcaaaag tggataagga tgatcaacct
      241 aaagataata aaaccaaacc tgaaaatcct ctaaaagaat taccaaaaac tggta
//
LOCUS       DAPIDM010000305          293 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000305 DAPIDM010000000
VERSION     DAPIDM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.315"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>293
                     /locus_tag="RJE42_002803"
     CDS             <1..>293
                     /locus_tag="RJE42_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDV5878132.1"
                     /translation="LCVSQIWESNRASAVVSGEKNPYVSESLKLNDNKSKSLSLEKYK
                     ESLRSIMCTKEINKNDGYDEPEYKEALDTYRKKLFAELDALNKFLDEERKIAS"
BASE COUNT          122 a           30 c           61 g           80 t
ORIGIN      
        1 ttatgtgtat cacaaatttg ggaaagcaat cgtgcgagtg cagtggtttc tggggagaag
       61 aatccatatg tatctgagtc tttgaaacta aatgataaca aaagtaaaag cttatcatta
      121 gaaaagtata aagagagtct tagaagtata atgtgtacaa aagaaatcaa caaaaatgat
      181 ggatatgatg agcctgaata taaagaagca ctggatactt acagaaaaaa attatttgct
      241 gagttagatg cacttaataa atttttagat gaagagagaa aaatagccag tta
//
LOCUS       DAPIDM010000306          287 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000306 DAPIDM010000000
VERSION     DAPIDM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.316"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>287
                     /locus_tag="RJE42_002804"
     CDS             <1..>287
                     /locus_tag="RJE42_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HDV5878133.1"
                     /translation="VDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDS
                     VPQIHGQNNGNQSFEEDTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHNEI"
BASE COUNT          113 a           42 c           62 g           70 t
ORIGIN      
        1 gttgatatta agagtgaatt aggttatgaa ggtggccaaa atagcggtaa ccagtcattc
       61 gaggaagaca cagaagaaga taaacctaaa tatgaacaag gtggtaatat cgtagatatc
      121 gatttcgaca gtgtacctca aattcatggt caaaataatg gtaaccagtc attcgaagaa
      181 gatacagaag aagacaagcc taagtatgaa caaggtggta acatcattga tatcgacttc
      241 gacagtgtgc cacaaattca tggattcaat aagcataatg aaattat
//
LOCUS       DAPIDM010000307          280 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000307 DAPIDM010000000
VERSION     DAPIDM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.317"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..>280
                     /locus_tag="RJE42_002805"
     CDS             <1..>280
                     /locus_tag="RJE42_002805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878134.1"
                     /translation="PALKWQPTDATSLTLLAEYQEDRTAYVYGLPAQGTVLPNINGKI
                     PRNRYTGEPGFDKFVMKRYSVGYLFEHAFTDQLKLRNSVRYFHADSTYF"
BASE COUNT           61 a          103 c           70 g           46 t
ORIGIN      
        1 cccggcgctc aagtggcagc ccaccgatgc cacctccctg acgctgctgg ccgaatacca
       61 ggaagaccgc acggcctatg tctatggcct gccggcccag ggcacggtgc tgccaaacat
      121 caatggcaag atcccccgca accgctacac cggcgaaccc ggcttcgaca aattcgtcat
      181 gaagcgctat tcggtcggct acctgttcga acacgcgttc acggaccagc tcaagctgcg
      241 caacagcgtg cgctacttcc acgcagacag cacctatttc
//
LOCUS       DAPIDM010000308          276 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000308 DAPIDM010000000
VERSION     DAPIDM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.318"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            91..173
                     /locus_tag="RJE42_002806"
     tRNA            91..173
                     /locus_tag="RJE42_002806"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Leu,seq:gag)
     gene            195..268
                     /locus_tag="RJE42_002807"
     tRNA            195..268
                     /locus_tag="RJE42_002807"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Gly,seq:tcc)
BASE COUNT           87 a           53 c           60 g           76 t
ORIGIN      
        1 aaaacgatta aaagttattg acaaatattt ttaatcataa tataataaat aacgttctga
       61 aaaacgaaga tgttttaaat gcctatatcc gcggtcgtgg cggaatggca gacgcgctag
      121 gttgagggcc tagtgggaga aatcccgtgg aggttcaaat cctctcggcc gcatcaaaat
      181 tcttaattaa ataagcgggt gtagtttaat ggcaaaacct cagccttcca agctgatgtt
      241 gtgggttcga ttcccatcac ccgctccata gataat
//
LOCUS       DAPIDM010000309          263 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000309 DAPIDM010000000
VERSION     DAPIDM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.denovo.319"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
BASE COUNT          114 a           40 c           21 g           88 t
ORIGIN      
        1 tttgcaatat caggtaaaga aacatttaaa accatttcat ttaatacact aaaaaatgaa
       61 agaatacaaa gccaaaataa aacgctataa aacaaacctt taaatttttt atataaacta
      121 aacaatttta cctctttact tgaggtgact aaagtttata ggggtaaatt attctttcac
      181 ctcatataaa ttccccaaat ttttaaatag acacttcata aaaaaatcct ccgaaattag
      241 aatagtttaa atatatcatt ttt
//
LOCUS       DAPIDM010000310         5090 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000310 DAPIDM010000000
VERSION     DAPIDM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.158"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            21..1391
                     /locus_tag="RJE42_002808"
     CDS             21..1391
                     /locus_tag="RJE42_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878135.1"
                     /translation="MDNKIEKILEDINKLQGYKVTYQYATAINIKDNNMNPWFEKIEE
                     ILLNVKGVIRILSPDNPPVNYDYWILPGRYKRHTKEYHEKLEKFLEKFATNANINDGL
                     FDQNNIHGYKYKKYIFTPHIEITHENTLNLFFLTLTIFNNGTFVIEIIEDLESINFGS
                     DLYSTFSCVKDKIYPLINQEKNNRQRQYQLYGNRHCKNIRDVSRQINRIIKINSKDIF
                     EYKVSKNMGFEVYYLANENEFTDDPFYEDVKIQEWLVNAPIFSFEGKSKHFRQDGYSD
                     NYINFLNKASVFVVWMDEQEEDVLEKCSQMALYFNSSANFFFLETMLNELQLYGYQQY
                     NLNGKKEALYFRQWILNFKRSIAIIYRTELLPSLYLYLYLKNNNDFKSPQYIEELNKE
                     ELSLIELEQSYKEEERSEKLNKLLLIISLLSIFQVVEIFFSDKNNIFNISMILIMILL
                     EVFRKR"
     gene            complement(1434..1844)
                     /locus_tag="RJE42_002809"
     CDS             complement(1434..1844)
                     /locus_tag="RJE42_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDV5878136.1"
                     /translation="MIPNPNAPDEYKYETDYRKIPRKYLNPKIPQGRGKIKWQAFATL
                     PQQFEILEQIIKDQNKIEKPLLTHDSLDNLDQIFQIKIQNDELCTISYWEDGNISKYT
                     GKILKKDEISNTFSFSDTNNNIYNLNNANVCSIT"
     gene            complement(2158..3003)
                     /gene="blaZ"
                     /locus_tag="RJE42_002810"
     CDS             complement(2158..3003)
                     /gene="blaZ"
                     /locus_tag="RJE42_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDV5878137.1"
                     /translation="MKKLILLIAIALVLSACNSTSSHAKELNNLEKKYNANIGVYALD
                     TKSGKEVKFNADKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMKYSDNTANNKIINEIGGIKKIKKRLKKLGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMLNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKNQSEPIILVIFTNKDNKSDKPNDKLISETAKN
                     VINKF"
     gene            3110..4867
                     /gene="blaR1"
                     /locus_tag="RJE42_002811"
     CDS             3110..4867
                     /gene="blaR1"
                     /locus_tag="RJE42_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDV5878138.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSFFKFNNLNNQEPTVESNSHNLNPNINTTKPVHEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLNSLLYLKYLKKQSLYLNEKEKDKINKILFNHQYKRNIV
                     IRKAESIHSPITFWYGKYIILIPSLYFKSINDKKLKYIILHEYAHAKNRDTLHLIIFH
                     IFSIAMSYNPLIQIVKRKMIHDNEVEADRFVLNNINKNEFKSYAEAIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKRRLINIKEGNLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHNHYPFDSWNKDQDLNTAIQNSVNWYFERISNQLS
                     KNYTSDQLKQLNYGNKNLGSYKAYWLEDSLKISNLEQVIVLKNMMEQNNHFSKNEKKQ
                     LSSSLLIRKNENYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFSTHLSDEKASGE
                     NAKFINEKILKEMGVLNGQ"
     gene            4857..>5090
                     /locus_tag="RJE42_002812"
     CDS             4857..>5090
                     /locus_tag="RJE42_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDV5878139.1"
                     /translation="MANKQVEISMAEWDVMNIIWNKKSVSANEIVVEIQKNKEVSDKT
                     IRTLITRLYKKEIIKRYKYNNIYFYSSIIKEDDI"
BASE COUNT         1929 a          574 c          688 g         1899 t
ORIGIN      
        1 gtatgaggat atttaaaata atggacaata aaattgaaaa gattctagaa gatattaata
       61 aattacaagg ctataaagta acatatcaat atgctactgc tataaatata aaagataata
      121 acatgaaccc ttggtttgaa aaaattgagg aaattctatt gaatgtaaaa ggagttatac
      181 gcattttatc tcctgacaac cccccggtaa attatgatta ttggatttta cctggaagat
      241 ataaaagaca tacaaaagaa tatcacgaaa aactagaaaa atttttagaa aaatttgcta
      301 ctaatgcaaa tattaatgat ggattgtttg atcaaaataa tatacatgga tacaaatata
      361 aaaaatatat attcactcca catattgaaa ttactcatga gaacactttg aatttatttt
      421 ttttaacctt aactatattt aacaatggta catttgttat agaaatcatt gaagacttag
      481 agagtataaa ctttggaagc gacttgtata gtactttctc atgtgtaaaa gataagatat
      541 atcctctaat taatcaagaa aaaaataata gacaaagaca gtatcaattg tatggtaata
      601 gacattgtaa aaatattaga gatgtttcta gacaaattaa tagaattata aaaattaata
      661 gtaaggatat ttttgaatat aaagtatcta aaaatatggg ttttgaagtt tattatttag
      721 ctaatgaaaa tgaatttact gatgatcctt tctatgagga cgtgaagatt caagagtggt
      781 tagtaaatgc gcctattttt tcatttgaag gaaaaagtaa acattttagg caagatggtt
      841 atagcgataa ttatataaac ttcttgaata aagcatctgt ttttgtagtt tggatggatg
      901 agcaagaaga agatgtttta gaaaaatgtt ctcaaatggc actttatttc aatagttctg
      961 ctaatttctt tttcttagaa actatgttaa atgaattaca attgtatggg tatcaacaat
     1021 ataatttaaa tggaaaaaaa gaagccctat attttagaca atggatacta aatttcaaga
     1081 gatctattgc aattatttat agaacagagc ttttacctag tttatattta tatttgtatc
     1141 taaagaataa taatgatttt aaatctccac aatatattga agaattaaac aaagaagaat
     1201 tatcactaat tgaattggag cagtcatata aggaagaaga aagaagcgaa aaattaaata
     1261 aacttttgtt aataataagt cttttatcaa ttttccaagt agtagaaatt tttttcagtg
     1321 ataaaaataa tatatttaat atcagtatga tacttataat gattttgcta gaagtatttc
     1381 gaaaaaggta attataagaa cactatttaa actttttaaa agtttaaata gtgttaagtg
     1441 atactacaaa cgttagcatt atttaaatta tatatattat tgttagtatc agaaaaggaa
     1501 aatgtattac taatttcatc cttttttaaa atttttcctg tatattttga aatgttaccg
     1561 tcttcccaat atgagattgt acataactca tcattttgaa tttttatttg aaaaatctga
     1621 tctaaattat ctaatgaatc atgtgttagc aatggttttt ctattttatt ttgatccttt
     1681 ataatttgtt ctaaaatttc aaactgttgc gggagagtcg caaaagcttg ccatttgatt
     1741 tttccacgcc cttgagggat tttagggttt aaatattttc ttggtatttt tctataatca
     1801 gtttcatatt tatattcatc tggcgcatta ggatttggaa tcatgtttac cacctcatca
     1861 tcattgtaga acgtatgttc ttttttggca aggattatat aattttttaa tgtgattaat
     1921 taattaattt aaaataagtt attttctata tggaaaagtt gagtaagatt atcgagcatt
     1981 tataattata gtaactggaa ttatgatgtt tggtatgtta atagtatttt ctcttttaaa
     2041 aaatgtgaac ataaaaaaat agaacatata taattataaa taatatatat gttcttcgat
     2101 attatgaagt tttatgaaag atagaattat atcagttttt gtagtacatt tagactatta
     2161 aaatttgttt attacattct tggcggtttc acttatcaat ttatcattag gtttatcact
     2221 tttattgtct ttattcgtaa aaatgactaa aataataggt tcagattggt tcttaggata
     2281 aacaaaagct acatcatttc tagaagcata tgttattgct tgaccacttt tatcagcaac
     2341 cttatagtct tttggaacac catctttaat taaagtgtct ccgtttttat tatttaacat
     2401 taaatcaagt aagaaatttt tatttttttt gcttaatttt ccatttgcga taagtttatt
     2461 taaagtcttg ccgaaagcag caggcgttga agtatctttt ttgctctttg gtgaatagta
     2521 atttaattct atttcatatc taactggatt tgttacttta tctcccaatt tttttaaacg
     2581 ttttttaatt tttttgattc caccgatttc gtttataatt ttattgtttg ctgtattatc
     2641 actatacttc attgaagcct caataagttc ttttaaagtg atatcttttc ctacatattt
     2701 ttctaaaata ggagaataag caactatatc atctttgtta atatgtactt ttttatttaa
     2761 cttattataa ggtacttgtt ctaacaaaat agcactattt atcgcttttg aagttgaagc
     2821 ataggcaaat ctcttatcgg cattaaattt tacttcctta ccacttttag tatctaatgc
     2881 atagacacca atattagcat tatatttctt ttctaaatta tttaactctt tagcatgtga
     2941 actggttgaa ttacatgcac ttaaaactaa agcaattgca attaaaagta ttaacttttt
     3001 caaaatatac cctccaatat tacagttgta atttctataa atcaataata ttacaattgt
     3061 aatattcgcg tcaatagttt attataaata tttgaaagaa ggtgttgaaa tggctaagtt
     3121 attaataatg agcatagtaa gcttttgttt tatatttcta ttgttattat tttttaggta
     3181 catattaaaa cgctatttta attatatgtt aaattataaa gtttggtatc taactcttct
     3241 tgcaggatta attcctttca tccctattaa attctctttt tttaaattta ataatttgaa
     3301 taatcaagag cccacagttg aaagtaattc acacaatttg aatcctaaca taaataccac
     3361 taaacctgtt catgagttta caacagacat ccataagttt aattgggatt caatagataa
     3421 tatctgcaca gttatttgga tagttttagt tattatttta agttttaaat ttttaaattc
     3481 tttattatat cttaagtatt taaagaaaca gtcactttat ctaaatgaaa aagaaaaaga
     3541 taaaataaac aagatacttt tcaatcatca atataaaaga aatattgtga ttcgaaaagc
     3601 agaatctatt cattctccaa taactttttg gtatggaaaa tatattattt tgattcccag
     3661 tttatatttt aaaagtataa atgacaaaaa actaaaatat ataatattgc acgagtatgc
     3721 tcatgctaaa aacagagata ctttgcattt aattatcttt catatttttt ctattgctat
     3781 gagttataat ccactaatac aaattgtaaa aagaaagatg attcatgaca atgaagtaga
     3841 agccgataga tttgtcctta ataatattaa caaaaatgaa tttaagtctt atgcggaagc
     3901 tattatggat tccgtattaa aaacctcatt tttcaacaaa aatatattaa gccattcatt
     3961 taatggtaaa aaatcattac tcaaaagaag attaattaat ataaaagaag gcaatctgaa
     4021 gaagcagtca aaattaattc taatttttat ttgtattttt acttttttta tcatgataat
     4081 tcaaagccag tttttaatgg ggcaatcctt aactgattat aattataaaa agcccttaca
     4141 aagtgattac caaattttag atgaaagtaa aaattttggt tcaaatagtg gatctttcgt
     4201 tatgtacagc atgaagaaag acaaatacta tatttataat gaaaaagaaa gtagaaaaag
     4261 atattctcct gattcaactt acaaaattta tttagcactg tttggacttg accgtcatat
     4321 tataagtgat aaaaattcac gtatgtcatg gaatcataat cattatcctt tcgattcttg
     4381 gaataaggat caagatttaa atacggcaat acaaaactca gttaattggt acttcgaacg
     4441 tattagtaat caattatcaa agaactatac ttctgatcaa ctcaagcaat taaattatgg
     4501 taataaaaat ttgggaagtt ataaagccta ttggttggaa gatagtttga aaatatctaa
     4561 tcttgaacaa gtaatagttt taaaaaatat gatggaacaa aataaccatt ttagtaaaaa
     4621 tgaaaagaaa caattatctt cttcgttatt gattagaaaa aatgaaaatt atgaattgta
     4681 tgggaaaaca ggtacaggta tagtaaacgg gaaatataat aatgggtggt ttgtaggtta
     4741 cgtaattaca aatcatgata agtattattt ttctacacat ttatcagatg aaaaagcctc
     4801 tggtgaaaat gctaaattca taaatgaaaa aatattaaaa gaaatgggtg ttttaaatgg
     4861 ccaataagca agttgaaata tctatggctg aatgggatgt tatgaatata atatggaata
     4921 aaaaatcagt atcagctaat gaaattgtag ttgagattca aaaaaataaa gaagttagcg
     4981 ataagacgat tagaacatta attacaagac tatataaaaa agagattata aaacgctata
     5041 aatataataa tatttatttt tactcatcaa ttattaaaga agatgatatt
//
LOCUS       DAPIDM010000311         4922 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.163, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000311 DAPIDM010000000
VERSION     DAPIDM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.163"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            16..972
                     /locus_tag="RJE42_002813"
     CDS             16..972
                     /locus_tag="RJE42_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDV5878140.1"
                     /translation="MVFSLLLFVAILFSDEQDSGISNIHYGGVNVSAEVLAHKPMVEK
                     YAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSLSTEESIKQGVKYFSE
                     LLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQSFSKEYSGGEKVSYPN
                     PIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDEALKYEGWRYVYGGAS
                     PTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSY
                     ITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            969..1901
                     /locus_tag="RJE42_002814"
     CDS             969..1901
                     /locus_tag="RJE42_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV5878141.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            2146..2262
                     /locus_tag="RJE42_002815"
     CDS             2146..2262
                     /locus_tag="RJE42_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDV5878142.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2278..4197
                     /gene="tet(M)"
                     /locus_tag="RJE42_002816"
     CDS             2278..4197
                     /gene="tet(M)"
                     /locus_tag="RJE42_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDV5878143.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4316..4483
                     /locus_tag="RJE42_002817"
     CDS             4316..4483
                     /locus_tag="RJE42_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDV5878144.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4543..4896)
                     /locus_tag="RJE42_002818"
     CDS             complement(4543..4896)
                     /locus_tag="RJE42_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV5878145.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         1626 a          818 c          996 g         1482 t
ORIGIN      
        1 tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat
       61 gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg
      121 ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc
      181 aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag
      241 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
      301 ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa
      361 tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga
      421 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa
      481 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc
      541 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
      601 acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt
      661 ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa
      721 gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca
      781 ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct
      841 tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca
      901 atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga
      961 atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa
     1021 aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg
     1081 tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta
     1141 cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata
     1201 ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca tgggagcaat
     1261 ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc
     1321 aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct gtaagaggat
     1381 ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc tacagtgtag
     1441 accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat atagtgagtg
     1501 tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt accaacatac
     1561 ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt gattccatta
     1621 caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag
     1681 ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca
     1741 tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc acggtatcgc
     1801 tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg
     1861 tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt
     1921 acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc tttacaaata
     1981 tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag
     2041 gtattcttaa ataaactgtc aatttgatag tgggaacaaa taattggatg tcctttttta
     2101 ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat
     2161 ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataaa
     2221 agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg
     2281 aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa
     2341 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
     2401 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
     2461 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
     2521 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
     2581 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
     2641 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
     2701 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
     2761 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
     2821 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
     2881 caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca
     2941 aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt ttattcatca
     3001 acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa
     3061 agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
     3121 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
     3181 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
     3241 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
     3301 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
     3361 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
     3421 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     3481 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
     3541 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
     3601 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
     3661 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata
     3721 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
     3781 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     3841 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     3901 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     3961 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     4021 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     4081 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     4141 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     4201 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     4261 agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg
     4321 tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat taaaaaaatt
     4381 ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc agttcaaagt
     4441 aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga ttaatacaat
     4501 ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt cgatgtttct
     4561 tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt ctgtaaagct
     4621 atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa
     4681 gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa cttgtatgct
     4741 ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt caatcaatgc
     4801 tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg ctaaaccaaa
     4861 ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct atacatttta
     4921 tt
//
LOCUS       DAPIDM010000312         3096 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000312 DAPIDM010000000
VERSION     DAPIDM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.196"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            46..2055
                     /gene="mecA"
                     /locus_tag="RJE42_002819"
     CDS             46..2055
                     /gene="mecA"
                     /locus_tag="RJE42_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDV5878146.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLRDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2101..2529)
                     /locus_tag="RJE42_002820"
     CDS             complement(2101..2529)
                     /locus_tag="RJE42_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDV5878147.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2626..>3096)
                     /locus_tag="RJE42_002821"
     CDS             complement(2626..>3096)
                     /locus_tag="RJE42_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDV5878148.1"
                     /translation="TFDDVVKIANEYNMNLNVELKGITGPNGLALSKSMVKQVEEQLT
                     NLNQNQEVLISSFNVVLVKLAEEIMPQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTE
                     DAKLTKAKVKMVKEAGYELNVWTVNKPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1161 a          460 c          494 g          981 t
ORIGIN      
        1 tacatttgta atatattaca aatgtagtct tatataagga ggatattgat gaaaaagata
       61 aaaattgttc cacttatttt aatagttgta gttgtcgggt ttggtatata tttttatgct
      121 tcaaaagata aagaaattaa taatactatt gatgcaattg aagataaaaa tttcaaacaa
      181 gtttataaag atagcagtta tatttctaaa agcgataatg gtgaagtaga aatgactgaa
      241 cgtccgataa aaatatataa tagtttaggc gttaaagata taaacattca ggatcgtaaa
      301 ataaaaaaag tatctaaaaa taaaaaacga gtagatgctc aatataaaat taaaacaaac
      361 tacggtaaca ttgatcgcaa cgttcaattt aattttgtta aagaagatgg tatgtggaag
      421 ttagattggg atcatagcgt cattattcca ggaatgcaga aagaccaaag catacatatt
      481 gaaaatttaa aatcagaacg tggtaaaatt ttagaccgaa acaatgtgga attggccaat
      541 acaggaacag catatgagat aggcatcgtt ccaaagaatg tatctaaaaa agattataaa
      601 gcaatcgcta aagaactaag tatttctgaa gactatatca aacaacaaat ggatcaaaat
      661 tgggtacaag atgatacctt cgttccactt aaaaccgtta aaaaaatgga tgaatattta
      721 agagatttcg caaaaaaatt tcatcttaca actaatgaaa cagaaagtcg taactatcct
      781 ctaggaaaag cgacttcaca tctattaggt tatgttggtc ccattaactc tgaagaatta
      841 aaacaaaaag aatataaagg ctataaagat gatgcagtta ttggtaaaaa gggactcgaa
      901 aaactttacg ataaaaagct ccaacatgaa gatggctatc gtgtcacaat cgttgacgat
      961 aatagcaata caatcgcaca tacattaata gagaaaaaga aaaaagatgg caaagatatt
     1021 caactaacta ttgatgctaa agttcaaaag agtatttata acaacatgaa aaatgattat
     1081 ggctcaggta ctgctatcca ccctcaaaca ggtgaattat tagcacttgt aagcacacct
     1141 tcatatgacg tctatccatt tatgtatggc atgagtaacg aagaatataa taaattaacc
     1201 gaagataaaa aagaacctct gctcaacaag ttccagatta caacttcacc aggttcaact
     1261 caaaaaatat taacagcaat gattgggtta aataacaaaa cattagacga taaaacaagt
     1321 tataaaatcg atggtaaagg ttggcaaaaa gataaatctt ggggtggtta caacgttaca
     1381 agatatgaag tggtaaatgg taatatcgac ttaaaacaag caatagaatc atcagataac
     1441 attttctttg ctagagtagc actcgaatta ggcagtaaga aatttgaaaa aggcatgaaa
     1501 aaactaggtg ttggtgaaga tataccaagt gattatccat tttataatgc tcaaatttca
     1561 aacaaaaatt tagataatga aatattatta gctgattcag gttacggaca aggtgaaata
     1621 ctgattaacc cagtacagat cctttcaatc tatagcgcat tagaaaataa tggcaatatt
     1681 aacgcacctc acttattaaa agacacgaaa aacaaagttt ggaagaaaaa tattatttcc
     1741 aaagaaaata tcaatctatt aactgatggt atgcaacaag tcgtaaataa aacacataaa
     1801 gaagatattt atagatctta tgcaaactta attggcaaat ccggtactgc agaactcaaa
     1861 atgaaacaag gagaaactgg cagacaaatt gggtggttta tatcatatga taaagataat
     1921 ccaaacatga tgatggctat taatgttaaa gatgtacaag ataaaggaat ggctagctac
     1981 aatgccaaaa tctcaggtaa agtgtatgat gagctatatg agaacggtaa taaaaaatac
     2041 gatatagatg aataacaaaa cagtgaagca atccgtaacg atggttgctt cactgtttta
     2101 ttatgaatta ttaataagtg ctgttacttc tcccttaaat acaatttctt cattttcatt
     2161 gtatgttgaa agtgacactg taacgagtcc attttctttt tttatggatt tcttatttgt
     2221 aatttcagcg ataacgtaca atgtattacc tgggtataca ggtttaataa atttaacgtt
     2281 attcatttgt gttcctgcta caacttcttc tccgtattta ccttcttcta cccataattt
     2341 aaatgatatt gaaagtgtat gcatgccaga tgcaatgata cctttaaatc tactttgttc
     2401 tgctttttct ttatctatat gcatatattg aggatcaaaa gttgttgcaa attggataat
     2461 ttcttcttct gtaatatgaa ggctttttgt tttgaatgtt tctcctacta taaaatcatc
     2521 gtatttcata tatgtctctc tttcttattc aaattaattt tttagtatgt aacatgttaa
     2581 aggtaagtct accgtcactg aaacgtaaga ctcacctcta actttctatt gagacaaatg
     2641 caccatttta tctgcattgt ctgtaaagat accatcaact ccccaattag caagttggtt
     2701 tgcacgtgct ggtttgttta cagtccatac gttcaattca taacccgctt cttttaccat
     2761 ttttactttt gctttagtaa gtttggcatc ttcagtgttt actattttag cattacagta
     2821 atctaaaagt gttctccagt cttcacgaaa cgaagttgta tggaatataa ctgctctgtt
     2881 atattgtggc atgatttctt ctgcaagttt aacaagcaca acattaaagc ttgaaatgag
     2941 cacttcttga ttctgattta agtttgttaa ttgttcttcc acttgcttaa ccatactttt
     3001 agaaagtgct agtccattcg gtccagtaat accttttaat tctacattta aattcatatt
     3061 atattcattt gctattttta ctacatcatc gaaagt
//
LOCUS       DAPIDM010000313         3095 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000313 DAPIDM010000000
VERSION     DAPIDM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.197"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            46..2054
                     /gene="mecA"
                     /locus_tag="RJE42_002822"
                     /pseudo
     CDS             46..2054
                     /gene="mecA"
                     /locus_tag="RJE42_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
     gene            complement(2100..2528)
                     /locus_tag="RJE42_002823"
     CDS             complement(2100..2528)
                     /locus_tag="RJE42_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDV5878149.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2625..>3095)
                     /locus_tag="RJE42_002824"
     CDS             complement(2625..>3095)
                     /locus_tag="RJE42_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDV5878150.1"
                     /translation="TFDDVVKIANEYNMNLNVELKGITGPNGLALSKSMVKQVEEQLT
                     NLNQNQEVLISSFNVVLVKLAEEIMPQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTE
                     DAKLTKAKVKMVKEAGYELNVWTVNKPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1160 a          460 c          494 g          981 t
ORIGIN      
        1 tacatttgta atatattaca aatgtagtct tatataagga ggatattgat gaaaaagata
       61 aaaattgttc cacttatttt aatagttgta gttgtcgggt ttggtatata tttttatgct
      121 tcaaaagata aagaaattaa taatactatt gatgcaattg aagataaaaa tttcaaacaa
      181 gtttataaag atagcagtta tatttctaaa agcgataatg gtgaagtaga aatgactgaa
      241 cgtccgataa aaatatataa tagtttaggc gttaaagata taaacattca ggatcgtaaa
      301 ataaaaaaag tatctaaaaa taaaaaacga gtagatgctc aatataaaat taaaacaaac
      361 tacggtaaca ttgatcgcaa cgttcaattt aattttgtta aagaagatgg tatgtggaag
      421 ttagattggg atcatagcgt cattattcca ggaatgcaga aagaccaaag catacatatt
      481 gaaaatttaa aatcagaacg tggtaaaatt ttagaccgaa acaatgtgga attggccaat
      541 acaggaacag catatgagat aggcatcgtt ccaaagaatg tatctaaaaa agattataaa
      601 gcaatcgcta aagaactaag tatttctgaa gactatatca aacaacaaat ggatcaaaat
      661 tgggtacaag atgatacctt cgttccactt aaaaccgtta aaaaaatgga tgaatattta
      721 agagatttcg caaaaaaatt tcatcttaca actaatgaaa cagaaagtcg taactatcct
      781 ctaggaaaag cgacttcaca tctattaggt tatgttggtc ccattaactc tgaagaatta
      841 aaacaaaaag aatataaagg ctataaagat gatgcagtta ttggtaaaaa gggactcgaa
      901 aactttacga taaaaagctc caacatgaag atggctatcg tgtcacaatc gttgacgata
      961 atagcaatac aatcgcacat acattaatag agaaaaagaa aaaagatggc aaagatattc
     1021 aactaactat tgatgctaaa gttcaaaaga gtatttataa caacatgaaa aatgattatg
     1081 gctcaggtac tgctatccac cctcaaacag gtgaattatt agcacttgta agcacacctt
     1141 catatgacgt ctatccattt atgtatggca tgagtaacga agaatataat aaattaaccg
     1201 aagataaaaa agaacctctg ctcaacaagt tccagattac aacttcacca ggttcaactc
     1261 aaaaaatatt aacagcaatg attgggttaa ataacaaaac attagacgat aaaacaagtt
     1321 ataaaatcga tggtaaaggt tggcaaaaag ataaatcttg gggtggttac aacgttacaa
     1381 gatatgaagt ggtaaatggt aatatcgact taaaacaagc aatagaatca tcagataaca
     1441 ttttctttgc tagagtagca ctcgaattag gcagtaagaa atttgaaaaa ggcatgaaaa
     1501 aactaggtgt tggtgaagat ataccaagtg attatccatt ttataatgct caaatttcaa
     1561 acaaaaattt agataatgaa atattattag ctgattcagg ttacggacaa ggtgaaatac
     1621 tgattaaccc agtacagatc ctttcaatct atagcgcatt agaaaataat ggcaatatta
     1681 acgcacctca cttattaaaa gacacgaaaa acaaagtttg gaagaaaaat attatttcca
     1741 aagaaaatat caatctatta actgatggta tgcaacaagt cgtaaataaa acacataaag
     1801 aagatattta tagatcttat gcaaacttaa ttggcaaatc cggtactgca gaactcaaaa
     1861 tgaaacaagg agaaactggc agacaaattg ggtggtttat atcatatgat aaagataatc
     1921 caaacatgat gatggctatt aatgttaaag atgtacaaga taaaggaatg gctagctaca
     1981 atgccaaaat ctcaggtaaa gtgtatgatg agctatatga gaacggtaat aaaaaatacg
     2041 atatagatga ataacaaaac agtgaagcaa tccgtaacga tggttgcttc actgttttat
     2101 tatgaattat taataagtgc tgttacttct cccttaaata caatttcttc attttcattg
     2161 tatgttgaaa gtgacactgt aacgagtcca ttttcttttt ttatggattt cttatttgta
     2221 atttcagcga taacgtacaa tgtattacct gggtatacag gtttaataaa tttaacgtta
     2281 ttcatttgtg ttcctgctac aacttcttct ccgtatttac cttcttctac ccataattta
     2341 aatgatattg aaagtgtatg catgccagat gcaatgatac ctttaaatct actttgttct
     2401 gctttttctt tatctatatg catatattga ggatcaaaag ttgttgcaaa ttggataatt
     2461 tcttcttctg taatatgaag gctttttgtt ttgaatgttt ctcctactat aaaatcatcg
     2521 tatttcatat atgtctctct ttcttattca aattaatttt ttagtatgta acatgttaaa
     2581 ggtaagtcta ccgtcactga aacgtaagac tcacctctaa ctttctattg agacaaatgc
     2641 accattttat ctgcattgtc tgtaaagata ccatcaactc cccaattagc aagttggttt
     2701 gcacgtgctg gtttgtttac agtccatacg ttcaattcat aacccgcttc ttttaccatt
     2761 tttacttttg ctttagtaag tttggcatct tcagtgttta ctattttagc attacagtaa
     2821 tctaaaagtg ttctccagtc ttcacgaaac gaagttgtat ggaatataac tgctctgtta
     2881 tattgtggca tgatttcttc tgcaagttta acaagcacaa cattaaagct tgaaatgagc
     2941 acttcttgat tctgatttaa gtttgttaat tgttcttcca cttgcttaac catactttta
     3001 gaaagtgcta gtccattcgg tccagtaata ccttttaatt ctacatttaa attcatatta
     3061 tattcatttg ctatttttac tacatcatcg aaagt
//
LOCUS       DAPIDM010000314         2985 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.200, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000314 DAPIDM010000000
VERSION     DAPIDM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.200"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            67..1023
                     /locus_tag="RJE42_002825"
     CDS             67..1023
                     /locus_tag="RJE42_002825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV5878151.1"
                     /translation="MLNSFDAAYHSLCEEVLELGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1223..1702
                     /locus_tag="RJE42_002826"
     CDS             1223..1702
                     /locus_tag="RJE42_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDV5878152.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEDVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1717..2556
                     /gene="fakB2"
                     /locus_tag="RJE42_002827"
     CDS             1717..2556
                     /gene="fakB2"
                     /locus_tag="RJE42_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDV5878153.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2642..>2985
                     /gene="msrA"
                     /locus_tag="RJE42_002828"
     CDS             2642..>2985
                     /gene="msrA"
                     /locus_tag="RJE42_002828"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV5878154.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKK"
BASE COUNT         1082 a          444 c          529 g          930 t
ORIGIN      
        1 ataaagttaa attatataga actaaatata catactataa ataataaaaa tgaggtgtta
       61 tcgcatatgt tgaattcatt tgatgcagca tatcacagtc tttgtgaaga agttttagaa
      121 ctagggaata cacgaaatga tcgcacaaat acaggtacga tttcgaaatt tggtcatcaa
      181 cttcgctttg acttatctaa aggatttcca ctattaacga caaagaaagt ttcttttaaa
      241 ttagtagcaa ccgaattatt atggttcatt aaaggagata caaatatcca atacttatta
      301 aagtataata ataatatttg gaacgaatgg gcttttgaaa attatatcaa atcagacgag
      361 tataaaggtc cagatatgac agatttcggg catcgtgcat tgagtgatcc tgaatttaac
      421 gaacaatata aagaacaaat gaaacaattt aagcaacgta ttcttgaaga tgatacattt
      481 gcgaagcaat tcggtgattt aggaaatgtt tatggtaaac agtggcgaga ttgggttgat
      541 aaagatggta atcattttga tcaacttaaa acagtaattg agcaaattaa gcataatcca
      601 gattcaagac gacacatcgt atctgcatgg aatccaacag aaattgacac aatggctctt
      661 ccgccttgtc ataccatgtt ccagttttat gtccaagatg gtaagttaag ttgccagtta
      721 taccaacgta gcgcagatat ctttttaggt gtgccattta atatcgcaag ctacgcttta
      781 ttgacacacc ttattgccaa agaatgtggc cttgaagtag gagaatttgt gcatactttt
      841 ggagatgcac atatttattc aaatcatatt gatgcgattc aaacacaatt agcacgtgaa
      901 agcttcaatc ctccaacatt aaaaattaac agtgataagt ctattttcga cataaattat
      961 gaagatttgg aaattgttga ctatgaatca catccagcaa taaaagctcc aatagcagtg
     1021 tagtcgttgc atagttagct aaccatatag acatcaaaat gacatcaaag tattttcaag
     1081 tgcaaaaaag tacttttttg tgttaaacgt tttcataaat tatgcaaaat cattatttct
     1141 atcacacttt atgataaaaa ttgtgttaaa ttaaagataa cttagtaata aaaaatgaaa
     1201 tgatagaaga aggaggataa ttatgacttt atccattcta gttgcacatg acttgcaacg
     1261 agtaattggt tttgaaaatc aattaccttg gcatctacca aatgatttga agcatgttaa
     1321 aaaattatca actggtcata ctttagtaat gggtcgtaag acatttgaat cgattggtaa
     1381 accactaccg aatcgtcgaa atgttgtact tacttcagat acaagtttca acgtagagga
     1441 cgttgatgta attcattcta ttgaagatat ttatcaacta ccgggccatg tttttatatt
     1501 tggagggcaa acattatttg aagaaatgat tgataaagtg gacgacatgt atattactgt
     1561 tattgaaggt aaatttcgtg gtgatacgtt ctttccacct tatacatttg aagactggga
     1621 agttgcctct tcagttgaag gtaaactaga tgagaaaaat acaattccac atacctttct
     1681 acatttaatt cgtaaaaaat aagggggaaa acgaccatga caaaacagat tatagtaaca
     1741 gactcaacat ccgatttatc taaagaatat ttagaagcaa acaacataca tgtaattcct
     1801 ttaagtttaa ctattgaagg agcttcatac gttgaccaag tagatattac atcagaagaa
     1861 tttattaatc atattgaaaa tgatgaagat gtaaagacaa gtcagccagc cataggtgaa
     1921 tttatatctg cttatgaaga actaggaaaa gatggctctg aaatcataag tattcatctt
     1981 tcttcaggac taagtggtac atttaacact gcttaccaag caagtcaaat ggtagatgct
     2041 aatgtaactg ttattgattc aaaatctatt tcttttggtt tagggtatca aatacaacac
     2101 ctagtagagc ttgtaaaaga aggtgtctca acttctgaaa tagttaaaaa gttaaatcat
     2161 ttaagagaaa acattaaatt atttgtagtt atagggcaat tgaatcaatt aattaaaggt
     2221 ggcagaatta gtaaaacaaa aggtttgatt ggtaatctta tgaaaattaa accaattggt
     2281 acactagatg atggtcgctt agagcttgtg cacaatgcga gaactcaaaa ttctagtatc
     2341 caatacttga aaaaggaaat tgctgaattt ataggagatc atgaaatcaa atccgttggt
     2401 gtcgcacatg ctaacgtcat tgaatatgtt gataaattga agaaagtttt taatgaagct
     2461 tttcatgtga ataattacga tataaatgta actacaccag ttatttctgc acatactggt
     2521 caaggtgcga ttggcctcgt agtccttaag aagtaaattt aatcttttca gtgttaatta
     2581 cttccatttc aatcctttat agactaaatt tataattaga tagatagagg aggtaattca
     2641 tatgacaaaa gaatatgcaa cattagcagg aggatgtttc tggtgcatgg ttaaaccatt
     2701 tacatcatat ccaggcatca agtcagtcgt atctggttat agtggcggtc atgttgataa
     2761 cccaacttat gaacaggtat gtacgaatca aaccggccat gtcgaagcag tacaaattac
     2821 gtttgatcca gaggttactt cctttgaaaa tatattagac atatatttca aaacatttga
     2881 cccaaccgat gatcaagggc aatttttcga tagaggcgaa agctatcaac cagtcatttt
     2941 ctatcatgat gagcatcaga aaaaggctgc tgagtttaaa aagca
//
LOCUS       DAPIDM010000315         2605 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000315 DAPIDM010000000
VERSION     DAPIDM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.211"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            complement(<1..149)
                     /locus_tag="RJE42_002829"
     CDS             complement(<1..149)
                     /locus_tag="RJE42_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV5878155.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSR"
     gene            385..1092
                     /gene="deoD"
                     /locus_tag="RJE42_002830"
     CDS             385..1092
                     /gene="deoD"
                     /locus_tag="RJE42_002830"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV5878156.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            1099..2451
                     /gene="tet(38)"
                     /locus_tag="RJE42_002831"
     CDS             1099..2451
                     /gene="tet(38)"
                     /locus_tag="RJE42_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDV5878157.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            2532..>2605
                     /locus_tag="RJE42_002832"
     CDS             2532..>2605
                     /locus_tag="RJE42_002832"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HDV5878158.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQI"
BASE COUNT          841 a          393 c          460 g          911 t
ORIGIN      
        1 atacgactga catcaaagcg cgcacaaagt tccttttcag taggtaatgc atggccactc
       61 ggatattttc ctatttgaat ttctttatat aacgtattat aaatcgttaa aaattttggt
      121 tgtgtttgcg tcacgtagac aacctccata aagttactta atcactctca tcatacaata
      181 atttttactc gaattggaaa aattataaaa attaaacata gaaacaactt gaacattggt
      241 tttatgcaag atcagtagag ataactgtaa aatgttctta atattgtcaa aatgtaatac
      301 ttgaaagcgc ttctaaaaaa tattattata tacatggtta gacaaataga caaattacta
      361 tacaaatatt gggaggaata ttttatgaaa tcaacaccac acattaaacc aatgaatgac
      421 gtcgaaattg cagaaacggt tctattgcca ggagatccgt taagagctaa gttcattgca
      481 gaaacatatt tggacgatgt ggaacagttc aatacagtgc gaaatatgtt tggttttacc
      541 ggaacatata aaggtaaaaa agtttctgtc atgggttcag gaatgggtat gccatctatt
      601 ggcatttact cttatgaatt aattcataca tttggttgta aaaaattaat tcgcgttggt
      661 tcttgtggcg cgatgcaaga aaacattgat ttatatgatg tgattattgc acaaggtgcc
      721 tctactgatt caaattacgt tcaacaatat caattaccag gtcattttgc gccaattgct
      781 tcttatcagt tattagaaaa agctgttgaa acagcacgtg acaaaggtgt acgtcatcat
      841 gtaggtaatg tgttatcaag tgatattttc tataacgcgg atacaacagc gagtgaacgt
      901 tggatgcgta tgggtatttt aggtgtagaa atggaatcag ctgcattata catgaatgca
      961 atttacgctg gtgtcgaagc attaggtgtg ttcacagtga gcgatcattt aattcatgaa
     1021 acgtcaacaa cacctgagga aagggaacgt gcatttacag atatgattga aattgcactg
     1081 tcattggtgt agatgattat gaatgttgaa tattctaaaa taaagaaagc agtacctatt
     1141 ttattattct tatttgtatt cagtttggtt atagacaact catttaaatt gatttctgta
     1201 gccattgctg atgacttaaa catttctgta acgacagtaa gttggcaagc gacattagcc
     1261 ggtttagtaa ttggtattgg cgctgtagta tacgcttcat tatctgatgc cattagtata
     1321 cgcacactat ttatttatgg cgtgatatta atcattatcg gatcaattat tggttacatt
     1381 ttccaacatc aattcgcatt acttttagtt ggacgtatta ttcaaactgc aggtttagct
     1441 gctgcagaga cattatatgt gatatatgtt gcaaagtatc tttctaaaga ggaccagaag
     1501 acttaccttg gcttaagtac gagcagttat tccttgtcat tagttatcgg tacattatca
     1561 ggtggattta tttctacgta tttacactgg acaaatatgt ttttaattgc attaatcgta
     1621 gtatttacgt tgccattcct atttaaatta ttaccaaaag aaaataatac aaataaagct
     1681 catttagatt ttgttggctt aattctagtg gcaactatcg ctacaacagt catgctgttt
     1741 attacgaact ttaattggtt atatatgatt ggtgctttaa ttgcgattat cgtttttgcg
     1801 ctatatatta aaaatgcgca acgtccatta gtaaacaaat catttttcca aaataaacgt
     1861 tatgcttcat ttttatttat agtatttgta atgtatgcca tccaattggg ttatattttt
     1921 acgttcccat tcataatgga acaaatttat catcttcagc tagacacaac atcactgcta
     1981 ttagtaccgg gttatattgt agcagtcatc gttggtgcac taagtggtaa aatcggcgaa
     2041 tatcttaatt caaaacaagc gattatcaca gcaattattt taatcgcact gagcttgatt
     2101 ttacctgcat ttgcagtagg taatcacatt tcaatcttcg tcatttctat gatattcttt
     2161 gcaggtagct ttgctttaat gtatgcacct ttacttaacg aagccattaa aacaatagat
     2221 cttaatatga ctggtgtggc tattggtttt tataatttaa ttattaatgt ggcggtatct
     2281 gtaggtattg cgattgctgc ggctctaatc gattttaaag cattaaattt cccaggcaat
     2341 gatgcattaa gttcacattt cggtattatt ttaattattt taggtttaat gagtattgtc
     2401 ggattagttt tattcgtcat cttaaatcgt tggacacaat ctgaaaaata aatagatata
     2461 aattcgcgag atatattcgt atttatagta aaattaaata aagagattat ataacacgag
     2521 gagtagtaag tatgaaattt gagaaatata tagatcacac tttattgaag cctgagtcaa
     2581 cacgtacgca aatcgatcaa atcat
//
LOCUS       DAPIDM010000316         2570 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000316 DAPIDM010000000
VERSION     DAPIDM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.214"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..899
                     /locus_tag="RJE42_002833"
     CDS             <1..899
                     /locus_tag="RJE42_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDV5878159.1"
                     /translation="KSLLKRRLINIKEGNLKKQSKLILIFICIFTFFIMIIQSQFLMG
                     QSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDS
                     TYKIYLALFGLDRHIISDKNSRMSWNHNHYPFDSWNKDQDLNTAIQNSVNWYFERISN
                     QLSKNYTSDQLKQLNYGNKNLGSYKAYWLEDSLKISNLEQVIVLKNMMEQNNHFSKNE
                     KKQLSSSLLIRKNENYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFSTHLSDEKA
                     SGENAKFINEKILKEMGVLNGQ"
     gene            889..1269
                     /gene="blaI"
                     /locus_tag="RJE42_002834"
     CDS             889..1269
                     /gene="blaI"
                     /locus_tag="RJE42_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDV5878160.1"
                     /translation="MANKQVEISMAEWDVMNIIWNKKSVSANEIVVEIQKNKEVSDKT
                     IRTLITRLYKKEIIKRYKYNNIYFYSSIIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(1321..1458)
                     /locus_tag="RJE42_002835"
     CDS             complement(1321..1458)
                     /locus_tag="RJE42_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV5878161.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFVICTNLIKNL
                     I"
     gene            1729..>2570
                     /locus_tag="RJE42_002836"
     CDS             1729..>2570
                     /locus_tag="RJE42_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HDV5878162.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTDTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVT"
BASE COUNT         1015 a          295 c          429 g          831 t
ORIGIN      
        1 aaaaatcatt actcaaaaga agattaatta atataaaaga aggcaatctg aagaagcagt
       61 caaaattaat tctaattttt atttgtattt ttactttttt tatcatgata attcaaagcc
      121 agtttttaat ggggcaatcc ttaactgatt ataattataa aaagccctta caaagtgatt
      181 accaaatttt agatgaaagt aaaaattttg gttcaaatag tggatctttc gttatgtaca
      241 gcatgaagaa agacaaatac tatatttata atgaaaaaga aagtagaaaa agatattctc
      301 ctgattcaac ttacaaaatt tatttagcac tgtttggact tgaccgtcat attataagtg
      361 ataaaaattc acgtatgtca tggaatcata atcattatcc tttcgattct tggaataagg
      421 atcaagattt aaatacggca atacaaaact cagttaattg gtacttcgaa cgtattagta
      481 atcaattatc aaagaactat acttctgatc aactcaagca attaaattat ggtaataaaa
      541 atttgggaag ttataaagcc tattggttgg aagatagttt gaaaatatct aatcttgaac
      601 aagtaatagt tttaaaaaat atgatggaac aaaataacca ttttagtaaa aatgaaaaga
      661 aacaattatc ttcttcgtta ttgattagaa aaaatgaaaa ttatgaattg tatgggaaaa
      721 caggtacagg tatagtaaac gggaaatata ataatgggtg gtttgtaggt tacgtaatta
      781 caaatcatga taagtattat ttttctacac atttatcaga tgaaaaagcc tctggtgaaa
      841 atgctaaatt cataaatgaa aaaatattaa aagaaatggg tgttttaaat ggccaataag
      901 caagttgaaa tatctatggc tgaatgggat gttatgaata taatatggaa taaaaaatca
      961 gtatcagcta atgaaattgt agttgagatt caaaaaaata aagaagttag cgataagacg
     1021 attagaacat taattacaag actatataaa aaagagatta taaaacgcta taaatataat
     1081 aatatttatt tttactcatc aattattaaa gaagatgata ttaaaatgaa aactgctaaa
     1141 acctttctta ataaattgta tggaggggat atgaaaagtt tagtgcttaa ttttgcgaaa
     1201 aatgaagaat taaataacaa agaaattgaa gaattgcgag acattttaaa tgatattagt
     1261 aaaaaataaa tagatatagg gttcaaacat aatacatata taaattatgt ttgaacccta
     1321 ttaaattaga ttttttatga gattagtaca tataacaaaa ggagcccatt taatcatgcc
     1381 tcttcccata ggtatattgt tatctagtaa ttctcttgga acgttacgat aatcagtttc
     1441 ttcttcataa cttgtcatcc ttaattcacc ccaatctgat aattacatta tacgaacatg
     1501 tgttctattt tgcaacaaaa attttgtgga agcataaacg cgttaataat taatgctcgg
     1561 gtaagtaaaa aagagggatt aattaaaatc gaataatgac atatcaccag caaatagttc
     1621 ttttaaagta gttaaacagt tttagcttta agggaatgat aaatgattgt taattctagc
     1681 taaaatttaa taaaatgaaa ataagactaa catggagggg taaaagtaat gacaaatgga
     1741 tatattggtt cttacactaa aaagaatggt aaagggattt atcgttttga attaaacgaa
     1801 aatcagtcac gtattgattt attagaaaca ggatttgaat tagaagcgtc tacatatttg
     1861 gtgcgtaata acgaagtttt atatggaatc aacaaagaag gagaacaatg cggtgttgcg
     1921 agtttgaaaa ttgatgacaa tggtgaattg catttattaa ataaatgttt gtcttcaaaa
     1981 gctggtacag gttgttatgt atcgatttca gaagataaac gatatttatt tgaagcggta
     2041 tatggtgctg gcatcatacg tatgtatgaa ttaaatacgg acacaggtga aattatacgt
     2101 ttaattcaag aacttgcgca tgattttcca acaggtacac atgaaagaca agatcatcca
     2161 cacgcacatt atattaatca aactccagat ggtaagtacg ttgcagtaac agatttaggt
     2221 gctgatcgta tcgttactta taaatttgat gacaacggtt ttgaatttta taaagaatct
     2281 ttatttaaag atagtgatgg gacaagacat attgaatttc atgataatgg aaaatttgct
     2341 tatgtcgtac acgaattatc aaatactgtg agcgttgcag aatataatga cggtaaattt
     2401 gaagagctcg agcgtcattt aacaattcct gaaaactttg atggagatac taaacttgca
     2461 gcagtgcgtt tatctcatga tcaacaattc ttatatgtat ctaatagagg gcatgatagc
     2521 attgcaattt ttaaagttct tgataatggt caacacttag aactagtaac
//
LOCUS       DAPIDM010000317         1877 bp    DNA     linear   BCT 12-SEP-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain 21CS0666
            SAMEA113401739-rid19406903.guided.234, whole genome shotgun
            sequence.
ACCESSION   DAPIDM010000317 DAPIDM010000000
VERSION     DAPIDM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113401739
            Sequence Read Archive: ERR11316191
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001881853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001881853.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/12/2023 12:00:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 36 of 76
            Pseudo Genes (incomplete)         :: 23 of 76
            Pseudo Genes (internal stop)      :: 29 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113401739-rid19406903.guided.234"
                     /strain="21CS0666"
                     /host="Bos taurus"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="FLI"
     gene            <1..1223
                     /gene="tet"
                     /locus_tag="RJE42_002837"
     CDS             <1..1223
                     /gene="tet"
                     /locus_tag="RJE42_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HDV5878163.1"
                     /translation="VNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAF
                     IGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLG
                     PSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIIC
                     FMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSI
                     VAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILG
                     SLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMS
                     LLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCL
                     LTIIVFKRSEKQFE"
     gene            1409..>1877
                     /locus_tag="RJE42_002838"
     CDS             1409..>1877
                     /locus_tag="RJE42_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDV5878164.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLN
                     YDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQ
                     FFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNK"
BASE COUNT          619 a          222 c          326 g          710 t
ORIGIN      
        1 gggtaaacac tgcatatatg ttaacttttt cgataggaac agcagtatat ggaaaattat
       61 ctgattatat aaatataaaa aaattgttaa ttattggtat tagtttgagc tgtcttggtt
      121 cattgattgc ttttattggt cacaatcact tttttatttt gatttttggt aggttagtac
      181 aaggagtagg atctgctgca ttcccttcac tgattatggt ggttgtagct agaaatatta
      241 caagaaaaaa acaaggcaaa gcctttggtt ttataggatc aattgtagct ttaggtgaag
      301 ggttaggtcc ttcaataggg ggaataatag cacattatat tcattggtct tacctactta
      361 tacttcctat gattacaata gtaactatac cttttcttat taaagtaatg gtacctggta
      421 aatcaacaaa aaatacatta gatatcgtag gtattgtttt aatgtctata agtattatat
      481 gttttatgtt atttacgaca aattataatt ggactttttt aatactcttc acaatctttt
      541 ttgtgatttt tattaaacat atttcaagag tttctaaccc ttttattaat cctaaactag
      601 ggaaaaacat tccgtttatg cttggtttgt tttctggtgg gctaatattt tctatagtag
      661 ctggttttat atcaatggtg ccttatatga tgaaaactat ttatcatgta aatgtagcga
      721 caataggtaa tagtgttatt tttcctggaa ccatgagtgt tattgttttt ggttattttg
      781 gtggtttttt agtggataga aaaggatcat tatttgtttt tattttagga tcattgtcta
      841 tctctataag ttttttaact attgcatttt ttgttgagtt tagtatgtgg ttgactactt
      901 ttatgtttat atttgttatg ggcggattat cttttactaa aacagttata tcaaaaatag
      961 tatcaagtag tctttctgaa gaagaagttg cttctggaat gagtttgcta aatttcacaa
     1021 gttttttatc agagggaaca ggtatagcaa ttgtaggagg tttattgtca ctacaattga
     1081 ttaatcgtaa actagttctg gaatttataa attattcttc tggagtgtat agtaatattc
     1141 ttgtagccat ggctatcctt attattttat gttgtctttt gacgattatt gtatttaaac
     1201 gttctgaaaa gcagtttgaa tagttatatt atattttggt ttagaactat gagtggctag
     1261 cattttgcca ctcatttttt gcgttagcaa aaacaggttt aagcctcgca gagcacacgt
     1321 attaacgact tattaaaaat aagtctagtg tattagactt aaactattaa atacacatga
     1381 aacctttgtg cttaggagtg atttttatat gtcttattcc attgttagag tttcaaaagt
     1441 taaatctgga acaaatacaa cgggcataca aaaacatgtt caaagagaaa ataataatta
     1501 tgaaaatgaa gatatagacc atagtaaaac ttacttaaat tatgatttgg taaatgctaa
     1561 taaacagaat tttaataact tgattgatga aaaaatcgaa cagaattata caggcaaaag
     1621 aaaaattaga acagacgcga ttaaacacat tgatggttta attacatcag acaatgattt
     1681 ctttgataat caaacgccag aagatacaaa gcagtttttt gaatatgcta aagagttttt
     1741 agaacaagaa tacggtaaag ataatttatt atatgcaaca gttcacatgg acgaaaaaac
     1801 accacatatg cattatggcg ttgttccaat aactgatgat ggtcgtttaa gtgctaaaga
     1861 agttgtaggt aataaaa
//
